show result file:crispr_blast.txt
Bacterium Spacer_Info Hit_ID Hit_Def Identity E-value Mismatch Detail_Info
NC_017545.1 Listeria monocytogenes J0161, complete genome 2.3|569024|NC_017545|36 NC_009812.1 Listeria phage B025, complete genome 97% 2e-10 1
NC_017545.1 Listeria monocytogenes J0161, complete genome 2.3|569024|NC_017545|36 NC_024387.1 Listeria phage LP-101, complete genome 94% 1e-08 2
NC_017545.1 Listeria monocytogenes J0161, complete genome 2.9|569414|NC_017545|36 NC_003216.1 Listeria phage A118, complete genome 100% 4e-12 0
NC_017545.1 Listeria monocytogenes J0161, complete genome 2.13|569671|NC_017545|34 NC_003216.1 Listeria phage A118, complete genome 100% 5e-11 0
NC_017545.1 Listeria monocytogenes J0161, complete genome 2.14|569734|NC_017545|36 NC_024384.1 Listeria phage LP-030-3, complete genome 100% 2e-10 0
NC_017545.1 Listeria monocytogenes J0161, complete genome 2.16|569864|NC_017545|36 NC_028929.1 Listeria phage vB_LmoS_293, complete genome 94% 1e-08 2
NC_017545.1 Listeria monocytogenes J0161, complete genome 2.25|570449|NC_017545|36 NC_028929.1 Listeria phage vB_LmoS_293, complete genome 100% 4e-12 0
NC_017545.1 Listeria monocytogenes J0161, complete genome 2.25|570449|NC_017545|36 NC_003216.1 Listeria phage A118, complete genome 97% 2e-10 1
NC_017545.1 Listeria monocytogenes J0161, complete genome 2.25|570449|NC_017545|36 NC_009810.1 Listeria phage A500, complete genome 94% 1e-08 2
NC_017545.1 Listeria monocytogenes J0161, complete genome 2.27|570577|NC_017545|36 NC_021539.2 Listeria phage LP-030-2, complete genome 97% 2e-10 1
NC_017545.1 Listeria monocytogenes J0161, complete genome 2.27|570577|NC_017545|36 NC_003291.2 Bacteriophage PSA complete genome 97% 2e-10 1
NC_017545.1 Listeria monocytogenes J0161, complete genome 4.4|2732333|NC_017545|30 NC_003216.1 Listeria phage A118, complete genome 100% 9e-09 0
NC_017545.1 Listeria monocytogenes J0161, complete genome 4.7|2732531|NC_017545|30 NC_009814.1 Listeria phage P35, complete genome 97% 5e-07 1
NC_017545.1 Listeria monocytogenes J0161, complete genome 4.8|2732597|NC_017545|30 NC_009810.1 Listeria phage A500, complete genome 97% 5e-07 1
NC_017545.1 Listeria monocytogenes J0161, complete genome 4.10|2732729|NC_017545|30 NC_024387.1 Listeria phage LP-101, complete genome 100% 3e-08 0
NC_017545.1 Listeria monocytogenes J0161, complete genome 4.11|2732795|NC_017545|30 NC_025440.1 Listeria phage WIL-1, complete genome 100% 9e-09 0
NC_017545.1 Listeria monocytogenes J0161, complete genome 4.11|2732795|NC_017545|30 NC_024787.1 Listeria phage LMTA-148, complete genome 100% 9e-09 0
NC_017545.1 Listeria monocytogenes J0161, complete genome 4.11|2732795|NC_017545|30 NC_024383.1 Listeria phage LP-083-2, complete genome 100% 9e-09 0
NC_017545.1 Listeria monocytogenes J0161, complete genome 4.11|2732795|NC_017545|30 NC_024360.1 Listeria phage LMSP-25, complete genome 100% 9e-09 0
NC_017545.1 Listeria monocytogenes J0161, complete genome 4.11|2732795|NC_017545|30 NC_024364.1 Listeria phage List-36, complete genome 100% 9e-09 0
NC_017545.1 Listeria monocytogenes J0161, complete genome 4.11|2732795|NC_017545|30 NC_024359.1 Listeria phage LP-048, complete genome 100% 9e-09 0
NC_017545.1 Listeria monocytogenes J0161, complete genome 4.11|2732795|NC_017545|30 NC_020871.1 Listeria phage vB_LmoM_AG20, complete genome 100% 9e-09 0
NC_017545.1 Listeria monocytogenes J0161, complete genome 4.11|2732795|NC_017545|30 NC_021781.2 Listeria phage LP-125, complete genome 97% 5e-07 1
NC_017545.1 Listeria monocytogenes J0161, complete genome 4.11|2732795|NC_017545|30 NC_009811.2 Listeria phage A511, complete genome 97% 5e-07 1
NC_017545.1 Listeria monocytogenes J0161, complete genome 4.12|2732861|NC_017545|30 NC_021781.2 Listeria phage LP-125, complete genome 100% 1e-07 0
NC_017545.1 Listeria monocytogenes J0161, complete genome 4.13|2732927|NC_017545|30 NC_028929.1 Listeria phage vB_LmoS_293, complete genome 100% 9e-09 0
NC_017545.1 Listeria monocytogenes J0161, complete genome 4.13|2732927|NC_017545|30 NC_009810.1 Listeria phage A500, complete genome 93% 0.001 2
NC_017545.1 Listeria monocytogenes J0161, complete genome 4.14|2732993|NC_017545|30 NC_028871.1 Listeria phage vB_LmoS_188, complete genome 100% 9e-09 0
NC_017545.1 Listeria monocytogenes J0161, complete genome 4.14|2732993|NC_017545|30 NC_028929.1 Listeria phage vB_LmoS_293, complete genome 100% 3e-08 0
NC_017545.1 Listeria monocytogenes J0161, complete genome 4.18|2733257|NC_017545|30 NC_003216.1 Listeria phage A118, complete genome 97% 5e-07 1
NC_017545.1 Listeria monocytogenes J0161, complete genome 2.3|569024|NC_017545.1|36 NC_009812.1 Listeria phage B025, complete genome 97% 2e-10 1
NC_017545.1 Listeria monocytogenes J0161, complete genome 2.3|569024|NC_017545.1|36 NC_024387.1 Listeria phage LP-101, complete genome 94% 1e-08 2
NC_017545.1 Listeria monocytogenes J0161, complete genome 2.9|569414|NC_017545.1|36 NC_003216.1 Listeria phage A118, complete genome 100% 4e-12 0
NC_017545.1 Listeria monocytogenes J0161, complete genome 2.13|569671|NC_017545.1|34 NC_003216.1 Listeria phage A118, complete genome 100% 5e-11 0
NC_017545.1 Listeria monocytogenes J0161, complete genome 2.14|569734|NC_017545.1|36 NC_024384.1 Listeria phage LP-030-3, complete genome 100% 2e-10 0
NC_017545.1 Listeria monocytogenes J0161, complete genome 2.16|569864|NC_017545.1|36 NC_028929.1 Listeria phage vB_LmoS_293, complete genome 94% 1e-08 2
NC_017545.1 Listeria monocytogenes J0161, complete genome 2.25|570449|NC_017545.1|36 NC_028929.1 Listeria phage vB_LmoS_293, complete genome 100% 4e-12 0
NC_017545.1 Listeria monocytogenes J0161, complete genome 2.25|570449|NC_017545.1|36 NC_003216.1 Listeria phage A118, complete genome 97% 2e-10 1
NC_017545.1 Listeria monocytogenes J0161, complete genome 2.25|570449|NC_017545.1|36 NC_009810.1 Listeria phage A500, complete genome 94% 1e-08 2
NC_017545.1 Listeria monocytogenes J0161, complete genome 2.27|570577|NC_017545.1|36 NC_021539.2 Listeria phage LP-030-2, complete genome 97% 2e-10 1
NC_017545.1 Listeria monocytogenes J0161, complete genome 2.27|570577|NC_017545.1|36 NC_003291.2 Bacteriophage PSA complete genome 97% 2e-10 1
NC_017545.1 Listeria monocytogenes J0161, complete genome 3.3|2732333|NC_017545.1|30 NC_003216.1 Listeria phage A118, complete genome 100% 9e-09 0
NC_017545.1 Listeria monocytogenes J0161, complete genome 3.6|2732531|NC_017545.1|30 NC_009814.1 Listeria phage P35, complete genome 97% 5e-07 1
NC_017545.1 Listeria monocytogenes J0161, complete genome 3.7|2732597|NC_017545.1|30 NC_009810.1 Listeria phage A500, complete genome 97% 5e-07 1
NC_017545.1 Listeria monocytogenes J0161, complete genome 3.9|2732729|NC_017545.1|30 NC_024387.1 Listeria phage LP-101, complete genome 100% 3e-08 0
NC_017545.1 Listeria monocytogenes J0161, complete genome 3.10|2732795|NC_017545.1|30 NC_025440.1 Listeria phage WIL-1, complete genome 100% 9e-09 0
NC_017545.1 Listeria monocytogenes J0161, complete genome 3.10|2732795|NC_017545.1|30 NC_024787.1 Listeria phage LMTA-148, complete genome 100% 9e-09 0
NC_017545.1 Listeria monocytogenes J0161, complete genome 3.10|2732795|NC_017545.1|30 NC_024383.1 Listeria phage LP-083-2, complete genome 100% 9e-09 0
NC_017545.1 Listeria monocytogenes J0161, complete genome 3.10|2732795|NC_017545.1|30 NC_024360.1 Listeria phage LMSP-25, complete genome 100% 9e-09 0
NC_017545.1 Listeria monocytogenes J0161, complete genome 3.10|2732795|NC_017545.1|30 NC_024364.1 Listeria phage List-36, complete genome 100% 9e-09 0
NC_017545.1 Listeria monocytogenes J0161, complete genome 3.10|2732795|NC_017545.1|30 NC_024359.1 Listeria phage LP-048, complete genome 100% 9e-09 0
NC_017545.1 Listeria monocytogenes J0161, complete genome 3.10|2732795|NC_017545.1|30 NC_020871.1 Listeria phage vB_LmoM_AG20, complete genome 100% 9e-09 0
NC_017545.1 Listeria monocytogenes J0161, complete genome 3.10|2732795|NC_017545.1|30 NC_021781.2 Listeria phage LP-125, complete genome 97% 5e-07 1
NC_017545.1 Listeria monocytogenes J0161, complete genome 3.10|2732795|NC_017545.1|30 NC_009811.2 Listeria phage A511, complete genome 97% 5e-07 1
NC_017545.1 Listeria monocytogenes J0161, complete genome 3.11|2732861|NC_017545.1|30 NC_021781.2 Listeria phage LP-125, complete genome 100% 1e-07 0
NC_017545.1 Listeria monocytogenes J0161, complete genome 3.12|2732927|NC_017545.1|30 NC_028929.1 Listeria phage vB_LmoS_293, complete genome 100% 9e-09 0
NC_017545.1 Listeria monocytogenes J0161, complete genome 3.12|2732927|NC_017545.1|30 NC_009810.1 Listeria phage A500, complete genome 93% 0.001 2
NC_017545.1 Listeria monocytogenes J0161, complete genome 3.13|2732993|NC_017545.1|30 NC_028871.1 Listeria phage vB_LmoS_188, complete genome 100% 9e-09 0
NC_017545.1 Listeria monocytogenes J0161, complete genome 3.13|2732993|NC_017545.1|30 NC_028929.1 Listeria phage vB_LmoS_293, complete genome 100% 3e-08 0
NC_017545.1 Listeria monocytogenes J0161, complete genome 3.17|2733257|NC_017545.1|30 NC_003216.1 Listeria phage A118, complete genome 97% 5e-07 1

Annotation:

spacer -> phage genome databese:
A viral reference database has been built for CRISPRs anlysis, it includes 10230 complete phage genomes from ncbi refSeq database. Putative protospacer targets will be identified by a BLASTN search of the spacer input against the viral reference database(default:coverage>=0.9), phage_ncbi_refseq_def_info.txt

phage genome -> spacer database:
Two spacer reference database has been built for CRISPRs anlysis, one only contains the complete bacteria genomes from NCBI, and the other includes the complete bacteria genomes and WGS bacteria genomes from NCBI.The best matching bacteria, which is obtained by aligning phage sequence gathered through user input to the spacer reference database(default:coverage>=0.9), is selected as the host of the predicted phage,all-bacteria-spacer.spc.

bacteria genome -> spacer -> phage genome:
Identify the spacer sequence from the bacteria sequence gathered from user input.
Determine whether the phage could infect the bacteria or not by aligning the identified spacer sequence to the phage sequence(default:coverage>0.9).