CRISPRimmunity

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Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Target MGE spacer number	Prophage number	Anti-CRISPR protein number
NZ_MWUW01000036	Staphylococcus delphini strain 214092504301-1 NODE_208_length_396_cov_100.32_ID_415, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_MWUW01000041	Staphylococcus delphini strain 214092504301-1 NODE_213_length_241_cov_204.868_ID_425, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_MWUW01000044	Staphylococcus delphini strain 214092504301-1 NODE_216_length_217_cov_97.4444_ID_431, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_MWUW01000013	Staphylococcus delphini strain 214092504301-1 NODE_13_length_48571_cov_102.604_ID_25, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_MWUW01000007	Staphylococcus delphini strain 214092504301-1 NODE_7_length_215749_cov_105.287_ID_13, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_MWUW01000010	Staphylococcus delphini strain 214092504301-1 NODE_10_length_110105_cov_103.717_ID_19, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_MWUW01000006	Staphylococcus delphini strain 214092504301-1 NODE_6_length_229728_cov_85.8656_ID_11, whole genome shotgun sequence	0 crisprs	DinG	0	0	0
NZ_MWUW01000005	Staphylococcus delphini strain 214092504301-1 NODE_5_length_229939_cov_98.2809_ID_9, whole genome shotgun sequence	0 crisprs	NA	0	2	0
NZ_MWUW01000030	Staphylococcus delphini strain 214092504301-1 NODE_32_length_997_cov_275.409_ID_63, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_MWUW01000001	Staphylococcus delphini strain 214092504301-1 NODE_1_length_303951_cov_90.7534_ID_1, whole genome shotgun sequence	0 crisprs	cas3	0	3	0
NZ_MWUW01000037	Staphylococcus delphini strain 214092504301-1 NODE_209_length_374_cov_680.854_ID_417, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_MWUW01000033	Staphylococcus delphini strain 214092504301-1 NODE_47_length_571_cov_201.786_ID_93, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_MWUW01000040	Staphylococcus delphini strain 214092504301-1 NODE_212_length_348_cov_80.8914_ID_423, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_MWUW01000028	Staphylococcus delphini strain 214092504301-1 NODE_29_length_1626_cov_95.0634_ID_57, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_MWUW01000025	Staphylococcus delphini strain 214092504301-1 NODE_26_length_2952_cov_486.327_ID_51, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_MWUW01000038	Staphylococcus delphini strain 214092504301-1 NODE_210_length_366_cov_69.2636_ID_419, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_MWUW01000008	Staphylococcus delphini strain 214092504301-1 NODE_8_length_153907_cov_97.2157_ID_15, whole genome shotgun sequence	1 crisprs	cas2,cas9,WYL	8	0	0
NZ_MWUW01000004	Staphylococcus delphini strain 214092504301-1 NODE_4_length_232048_cov_90.8293_ID_7, whole genome shotgun sequence	0 crisprs	cas3,DEDDh,csa3	0	1	0
NZ_MWUW01000018	Staphylococcus delphini strain 214092504301-1 NODE_18_length_30468_cov_109.523_ID_35, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_MWUW01000042	Staphylococcus delphini strain 214092504301-1 NODE_214_length_224_cov_78.732_ID_427, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_MWUW01000002	Staphylococcus delphini strain 214092504301-1 NODE_2_length_274319_cov_103.811_ID_3, whole genome shotgun sequence	0 crisprs	NA	0	1	2
NZ_MWUW01000031	Staphylococcus delphini strain 214092504301-1 NODE_37_length_717_cov_209.414_ID_73, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_MWUW01000034	Staphylococcus delphini strain 214092504301-1 NODE_153_length_454_cov_71.5872_ID_305, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_MWUW01000017	Staphylococcus delphini strain 214092504301-1 NODE_17_length_40317_cov_99.8221_ID_33, whole genome shotgun sequence	0 crisprs	cas3	0	0	0
NZ_MWUW01000026	Staphylococcus delphini strain 214092504301-1 NODE_27_length_1946_cov_844.179_ID_53, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_MWUW01000015	Staphylococcus delphini strain 214092504301-1 NODE_15_length_41013_cov_105.598_ID_29, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_MWUW01000021	Staphylococcus delphini strain 214092504301-1 NODE_21_length_8803_cov_134.004_ID_41, whole genome shotgun sequence	0 crisprs	NA	0	1	0
NZ_MWUW01000045	Staphylococcus delphini strain 214092504301-1 NODE_217_length_206_cov_177.646_ID_433, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_MWUW01000043	Staphylococcus delphini strain 214092504301-1 NODE_215_length_224_cov_200.371_ID_429, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_MWUW01000029	Staphylococcus delphini strain 214092504301-1 NODE_30_length_1290_cov_546.854_ID_59, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_MWUW01000019	Staphylococcus delphini strain 214092504301-1 NODE_19_length_21297_cov_94.1899_ID_37, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_MWUW01000039	Staphylococcus delphini strain 214092504301-1 NODE_211_length_366_cov_85.4393_ID_421, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_MWUW01000023	Staphylococcus delphini strain 214092504301-1 NODE_23_length_7459_cov_94.6605_ID_45, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_MWUW01000011	Staphylococcus delphini strain 214092504301-1 NODE_11_length_97191_cov_96.0004_ID_21, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_MWUW01000012	Staphylococcus delphini strain 214092504301-1 NODE_12_length_49095_cov_100.014_ID_23, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_MWUW01000032	Staphylococcus delphini strain 214092504301-1 NODE_45_length_584_cov_169.969_ID_89, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_MWUW01000024	Staphylococcus delphini strain 214092504301-1 NODE_24_length_6624_cov_58.7791_ID_47, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_MWUW01000035	Staphylococcus delphini strain 214092504301-1 NODE_207_length_399_cov_119.235_ID_413, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_MWUW01000016	Staphylococcus delphini strain 214092504301-1 NODE_16_length_40917_cov_101.664_ID_31, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_MWUW01000022	Staphylococcus delphini strain 214092504301-1 NODE_22_length_8086_cov_106.613_ID_43, whole genome shotgun sequence	0 crisprs	NA	0	1	0
NZ_MWUW01000009	Staphylococcus delphini strain 214092504301-1 NODE_9_length_118492_cov_94.8603_ID_17, whole genome shotgun sequence	0 crisprs	DEDDh	0	0	0
NZ_MWUW01000014	Staphylococcus delphini strain 214092504301-1 NODE_14_length_44146_cov_103.235_ID_27, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_MWUW01000020	Staphylococcus delphini strain 214092504301-1 NODE_20_length_13675_cov_117.309_ID_39, whole genome shotgun sequence	0 crisprs	NA	0	1	0
NZ_MWUW01000003	Staphylococcus delphini strain 214092504301-1 NODE_3_length_236951_cov_101.282_ID_5, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_MWUW01000027	Staphylococcus delphini strain 214092504301-1 NODE_28_length_1649_cov_469.9_ID_55, whole genome shotgun sequence	0 crisprs	NA	0	0	0

Results visualization

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CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

131733 : 140153

Synechococcus_phage(33.33%)

The bacterium proteins that are colored denote the protein is present at specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin' and 'tRNA')

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_096542962.1\|131733_132216_+	5-(carboxyamino)imidazole ribonucleotide mutase	A0A2P0VNU7	Tetraselmis_virus	5.2e-21	43.3
WP_096542960.1\|132202_133336_+	5-(carboxyamino)imidazole ribonucleotide synthase	NA	NA	NA	NA
WP_096542958.1\|133332_134034_+	phosphoribosylaminoimidazolesuccinocarboxamide synthase	G8EYA2	Synechococcus_phage	2.3e-49	44.2
WP_014614277.1\|134036_134297_+	phosphoribosylformylglycinamidine synthase subunit PurS	NA	NA	NA	NA
WP_096542956.1\|134298_134970_+	phosphoribosylformylglycinamidine synthase I	NA	NA	NA	NA
WP_096542954.1\|134962_137152_+	phosphoribosylformylglycinamidine synthase subunit PurL	A6N228	Microbacterium_phage	2.5e-139	40.4
WP_096542952.1\|137130_138552_+	amidophosphoribosyltransferase	A0A0M3SGR2	Mollivirus	7.1e-50	33.4
WP_096542950.1\|138555_139587_+	phosphoribosylformylglycinamidine cyclo-ligase	Q58MH8	Prochlorococcus_phage	1.5e-62	42.4
WP_096542948.1\|139586_140153_+	phosphoribosylglycinamide formyltransferase	M4QRX9	Synechococcus_phage	1.5e-27	37.8

DBSCAN-SWA_2

221497 : 229939

Staphylococcus_phage(62.5%)

integrase

attL 218953:218967|attR 228603:228617

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
218953:218967	attL	NA	NA	NA	NA
WP_096543109.1\|221497_222520_-\|integrase	site-specific integrase	A0A1Q1PVY8	Staphylococcus_phage	1.0e-114	62.3
WP_096543108.1\|222952_223639_-	Ltp family lipoprotein	A0A2H4J5B8	uncultured_Caudovirales_phage	3.2e-24	79.1
WP_096543107.1\|223696_224149_-	ImmA/IrrE family metallo-endopeptidase	A0A2I6PE92	Staphylococcus_phage	2.1e-56	66.2
WP_096543106.1\|224168_224492_-	helix-turn-helix transcriptional regulator	A0A2H4JC44	uncultured_Caudovirales_phage	2.7e-37	76.1
WP_096543105.1\|224683_224929_+	helix-turn-helix transcriptional regulator	A0A1J0MFJ4	Staphylococcus_phage	1.4e-35	93.5
WP_096543104.1\|224945_225830_+	DUF3102 domain-containing protein	A0A2H4J4L1	uncultured_Caudovirales_phage	2.2e-70	66.0
WP_096588975.1\|225842_226337_+	ORF6C domain-containing protein	A0A2H4J854	uncultured_Caudovirales_phage	1.2e-52	76.5
WP_096540481.1\|226371_226638_+	DUF2829 domain-containing protein	A0A2H4J8T7	uncultured_Caudovirales_phage	8.9e-15	54.9
WP_096540483.1\|226621_226939_-	hypothetical protein	NA	NA	NA	NA
WP_096540485.1\|227110_227254_+	hypothetical protein	Q4ZC31	Staphylococcus_virus	2.4e-14	93.6
WP_096540487.1\|227323_227554_-	hypothetical protein	NA	NA	NA	NA
WP_096540489.1\|227621_227849_+	DUF771 domain-containing protein	A0A1Q1PW00	Staphylococcus_phage	2.6e-23	71.0
WP_173672453.1\|227863_228040_+	hypothetical protein	A0A223LGE0	Staphylococcus_phage	2.9e-14	75.9
WP_096540491.1\|228118_228379_+	DUF1108 family protein	A0A1J0MFI6	Staphylococcus_phage	7.3e-38	91.9
WP_096540493.1\|228390_228654_+	hypothetical protein	A0A1J0MFA5	Staphylococcus_phage	2.5e-38	92.0
228603:228617	attR	NA	NA	NA	NA
WP_060830236.1\|228637_229123_+	siphovirus Gp157 family protein	A0A223LGF3	Staphylococcus_phage	5.0e-80	98.1
WP_015728804.1\|229123_229792_+	ERF family protein	A0A1J0MFT8	Staphylococcus_phage	1.0e-120	100.0
WP_142302270.1\|229791_229939_+	single-stranded DNA-binding protein	A0A1J0MFL8	Staphylococcus_phage	1.1e-17	91.8

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

2. NZ_MWUW01000008

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CRISPR-Cas detection and classification

Crispr_ID: NZ_MWUW01000008_1

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

NZ_MWUW01000008_1

25538-26022

TypeII

Consensus_repeat	Method
TTCTAAATAAACATAGTGCTAAAAC	CRT
GTTTTACTTCATTCTAAATAAACATAGTGCTAAAAC	CRISPRCasFinder
GTTTTACTTCATTCTAAATAAACATAGTGCTAAAAC	PILER-CR

7 spacers

cas2,cas9

The CRISPR arrays of NZ_MWUW01000008_1

>merge|NZ_MWUW01000008|1|25538-26022|CRT,CRISPRCasFinder,PILER-CR
TGAGGTGAAAGCATAGTGCGAGACGTAAATGTAGTTCGTATTTGATTTTTAAAAGGTTTGTTATACTTACTAAATAAACATAGTGCTAAAACGTATATACTGACTTCTTGCCTATCGGTACAGTTTACTTCATTCTAAATAAACATAGTGCTAAAACGTTACTTAATAAAATGTGCCACGATTTTAGTTTTACTTCATTCTAAATAAACATAGTGCTAAAACAAACATCCCTTTTGGAAATACGTATTTAAGTTTTACTTCTTTCTAAATAAACATAGTGCTAAAACTCAACTTGCGTCCCTCGTCACTTGTTTCGGTTTTACTTCATTCTAAATAAACATAGTGCTAAAACACTATGTCAGACCTCAAAGGAAAGATTCTTGTTTTACTTCATTCTAAATAAACATAGTGCTAAAACTCCGCTTGGTACTTAAAGCGAATCCACCACAGTTTTACTTCTTTCTAAATAAACATAGTGCTAAAAC

>NZ_MWUW01000008|1|1|25538-26022|CRT
TGAGGTGAAAGCATAGTGCGAGACG	TAAATGTAGTTCGTATTTGATTTTTAAAAGGTTTGTTATACT
TACTAAATAAACATAGTGCTAAAAC	GTATATACTGACTTCTTGCCTATCGGTACAGTTTACTTCA
TTCTAAATAAACATAGTGCTAAAAC	GTTACTTAATAAAATGTGCCACGATTTTAGTTTTACTTCA
TTCTAAATAAACATAGTGCTAAAAC	AAACATCCCTTTTGGAAATACGTATTTAAGTTTTACTTCT
TTCTAAATAAACATAGTGCTAAAAC	TCAACTTGCGTCCCTCGTCACTTGTTTCGGTTTTACTTCA
TTCTAAATAAACATAGTGCTAAAAC	ACTATGTCAGACCTCAAAGGAAAGATTCTTGTTTTACTTCA
TTCTAAATAAACATAGTGCTAAAAC	TCCGCTTGGTACTTAAAGCGAATCCACCACAGTTTTACTTCT
TTCTAAATAAACATAGTGCTAAAAC

>NZ_MWUW01000008|1|1|25594-26022|CRISPRCasFinder
TTTGTTATACTTACTAAATAAACATAGTGCTAAAAC	GTATATACTGACTTCTTGCCTATCGGTAC
AGTTTACTTCATTCTAAATAAACATAGTGCTAAAAC	GTTACTTAATAAAATGTGCCACGATTTTA
GTTTTACTTCATTCTAAATAAACATAGTGCTAAAAC	AAACATCCCTTTTGGAAATACGTATTTAA
GTTTTACTTCTTTCTAAATAAACATAGTGCTAAAAC	TCAACTTGCGTCCCTCGTCACTTGTTTCG
GTTTTACTTCATTCTAAATAAACATAGTGCTAAAAC	ACTATGTCAGACCTCAAAGGAAAGATTCTT
GTTTTACTTCATTCTAAATAAACATAGTGCTAAAAC	TCCGCTTGGTACTTAAAGCGAATCCACCACA
GTTTTACTTCTTTCTAAATAAACATAGTGCTAAAAC

>NZ_MWUW01000008|1|1|25660-26022|PILER-CR
GTTTACTTCATTCTAAATAAACATAGTGCTAAAACG	TTACTTAATAAAATGTGCCACGATTTTAG
TTTTACTTCATTCTAAATAAACATAGTGCTAAAACA	AACATCCCTTTTGGAAATACGTATTTAAG
TTTTACTTCTTTCTAAATAAACATAGTGCTAAAACT	CAACTTGCGTCCCTCGTCACTTGTTTCGG
TTTTACTTCATTCTAAATAAACATAGTGCTAAAACA	CTATGTCAGACCTCAAAGGAAAGATTCTTG
TTTTACTTCATTCTAAATAAACATAGTGCTAAAACT	CCGCTTGGTACTTAAAGCGAATCCACCACAG
TTTTACTTCTTTCTAAATAAACATAGTGCTAAAAC

Protein	Signature genes	Signature genes Name	Protein_function
NZ_MWUW01000008.1\|WP_142302274.1\|32868_33036_-\|sce7725-family-protein	unknown	unknown	unknown
NZ_MWUW01000008.1\|WP_096543248.1\|24403_25414_-\|zinc-binding-alcohol-dehydrogenase-family-protein	unknown	unknown	gnl\|CDD\|176214
NZ_MWUW01000008.1\|WP_096543277.1\|36807_37674_-\|energy-coupling-factor-transporter-ATPase	unknown	unknown	gnl\|CDD\|184205
NZ_MWUW01000008.1\|WP_096543244.1\|19991_21137_+\|MFS-transporter	unknown	unknown	gnl\|CDD\|225371
NZ_MWUW01000008.1\|WP_096543280.1\|33010_33883_-\|sce7726-family-protein	unknown	unknown	unknown
NZ_MWUW01000008.1\|WP_019165072.1\|21752_21998_-\|DUF2273-domain-containing-protein	unknown	unknown	gnl\|CDD\|227834
NZ_MWUW01000008.1\|WP_096543245.1\|21207_21699_-\|Asp23/Gls24-family-envelope-stress-response-protein	unknown	unknown	gnl\|CDD\|377129
NZ_MWUW01000008.1\|WP_096543242.1\|16627_17812_-\|MFS-transporter	unknown	unknown	gnl\|CDD\|340863
NZ_MWUW01000008.1\|WP_096543247.1\|22832_24347_-\|BCCT-family-transporter	unknown	unknown	gnl\|CDD\|376718
NZ_MWUW01000008.1\|WP_096543249.1\|27056_27401_-\|CRISPR-associated-endonuclease-Cas2	cas2	COG3512_cas2_CAS-II-A:CAS-II-B:CAS-II-C	gnl\|CDD\|226043
NZ_MWUW01000008.1\|WP_173672491.1\|22010_22553_-\|alkaline-shock-response-membrane-anchor-protein-AmaP	unknown	unknown	gnl\|CDD\|380217
NZ_MWUW01000008.1\|WP_096543278.1\|36014_36818_-\|energy-coupling-factor-transporter-transmembrane-protein-EcfT	unknown	unknown	gnl\|CDD\|223692
NZ_MWUW01000008.1\|WP_096589032.1\|28294_32299_-\|type-II-CRISPR-RNA-guided-endonuclease-Cas9	cas9	mkCas0193_cas9_CAS-II-A:CAS-II-B:CAS-II-C	gnl\|CDD\|273840
NZ_MWUW01000008.1\|WP_096543281.1\|32551_32857_-\|hypothetical-protein	unknown	unknown	gnl\|CDD\|225181
NZ_MWUW01000008.1\|WP_096543241.1\|14880_16644_-\|siderophore-synthetase	unknown	unknown	gnl\|CDD\|226715
NZ_MWUW01000008.1\|WP_096543279.1\|35195_35999_-\|tRNA-pseudouridine(38-40)-synthase-TruA	unknown	unknown	gnl\|CDD\|234577
NZ_MWUW01000008.1\|WP_096589030.1\|17912_19871_+\|sialic-acid-synthase	unknown	unknown	gnl\|CDD\|226715
NZ_MWUW01000008.1\|WP_096543240.1\|13800_14877_-\|alanine-racemase	unknown	unknown	gnl\|CDD\|143490
NZ_MWUW01000008.1\|WP_014613243.1\|34137_34536_-\|30S-ribosomal-protein-S9	unknown	unknown	gnl\|CDD\|178888
NZ_MWUW01000008.1\|WP_014613242.1\|34549_34987_-\|50S-ribosomal-protein-L13	unknown	unknown	gnl\|CDD\|181703

Protein	Function_ID	Function_description	E-value
NZ_MWUW01000008.1\|WP_096543248.1\|24403_25414_-\|zinc-binding-alcohol-dehydrogenase-family-protein	gnl\|CDD\|176214	cd08252, AL_MDR, Arginate lyase and other MDR family members. This group contains a structure identified as an arginate lyase. Other members are identified quinone reductases, alginate lyases, and other proteins related to the zinc-dependent dehydrogenases/reductases. QOR catalyzes the conversion of a quinone and NAD(P)H to a hydroquinone and NAD(P+. Quinones are cyclic diones derived from aromatic compounds. Membrane bound QOR acts in the respiratory chains of bacteria and mitochondria, while soluble QOR acts to protect from toxic quinones (e.g. DT-diaphorase) or as a soluble eye-lens protein in some vertebrates (e.g. zeta-crystalin). QOR reduces quinones through a semi-quinone intermediate via a NAD(P)H-dependent single electron transfer. QOR is a member of the medium chain dehydrogenase/reductase family, but lacks the zinc-binding sites of the prototypical alcohol dehydrogenases of this group. Alcohol dehydrogenase in the liver converts ethanol and NAD+ to acetaldehyde and NADH, while in yeast and some other microorganisms ADH catalyzes the conversion acetaldehyde to ethanol in alcoholic fermentation. ADH is a member of the medium chain alcohol dehydrogenase family (MDR), which has a NAD(P)(H)-binding domain in a Rossmann fold of a beta-alpha form. The NAD(H)-binding region is comprised of 2 structurally similar halves, each of which contacts a mononucleotide. The N-terminal catalytic domain has a distant homology to GroES. These proteins typically form dimers (typically higher plants, mammals) or tetramers (yeast, bacteria), and have 2 tightly bound zinc atoms per subunit, a catalytic zinc at the active site and a structural zinc in a lobe of the catalytic domain. NAD(H) binding occurs in the cleft between the catalytic and coenzyme-binding domains at the active site, and coenzyme binding induces a conformational closing of this cleft. Coenzyme binding typically precedes and contributes to substrate binding. In human ADH catalysis, the zinc ion helps coordinate the alcohol, followed by deprotonation of a histidine, the ribose of NAD, a serine, then the alcohol, which allows the transfer of a hydride to NAD+, creating NADH and a zinc-bound aldehyde or ketone. In yeast and some bacteria, the active site zinc binds an aldehyde, polarizing it, and leading to the reverse reaction.	1.80341e-155
NZ_MWUW01000008.1\|WP_096543277.1\|36807_37674_-\|energy-coupling-factor-transporter-ATPase	gnl\|CDD\|184205	PRK13646, cbiO, energy-coupling factor transporter ATPase.	1.38599e-152
NZ_MWUW01000008.1\|WP_096543244.1\|19991_21137_+\|MFS-transporter	gnl\|CDD\|225371	COG2814, AraJ, Arabinose efflux permease [Carbohydrate transport and metabolism].	8.16521e-73
NZ_MWUW01000008.1\|WP_019165072.1\|21752_21998_-\|DUF2273-domain-containing-protein	gnl\|CDD\|227834	COG5547, COG5547, Small integral membrane protein [Function unknown].	3.95841e-17
NZ_MWUW01000008.1\|WP_096543245.1\|21207_21699_-\|Asp23/Gls24-family-envelope-stress-response-protein	gnl\|CDD\|377129	pfam03780, Asp23, Asp23 family, cell envelope-related function. The alkaline shock protein Asp23 was identified as an alkaline shock protein that was expressed in a sigmaB-dependent manner in Staphylococcus aureus. Following an alkaline shock Asp23 accumulates in the soluble protein fraction of the S. aureus cell. Asp23 is one of the most abundant proteins in the cytosolic protein fraction of stationary S. aureus cells, with a copy-number of >25000 per cell. A second Asp23-family protein, AmaP, which is encoded within the asp23-operon, is required to localize Asp23 to the cell membrane. The overall function for the family is thus a cell envelope-related one in Gram-positive bacteria.	1.17688e-34
NZ_MWUW01000008.1\|WP_096543242.1\|16627_17812_-\|MFS-transporter	gnl\|CDD\|340863	cd06173, MFS_MefA_like, Macrolide efflux protein A and similar proteins of the Major Facilitator Superfamily of transporters. This family is composed of Streptococcus pyogenes macrolide efflux protein A (MefA) and similar transporters, many of which remain uncharacterized. Some members may be multidrug resistance (MDR) transporters, which are drug/H+ antiporters (DHAs) that mediate the efflux of a variety of drugs and toxic compounds, conferring resistance to these compounds. MefA confers resistance to 14-membered macrolides including erythromycin and to 15-membered macrolides. It functions as an efflux pump to regulate intracellular macrolide levels. The MefA-like family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.	1.94639e-54
NZ_MWUW01000008.1\|WP_096543247.1\|22832_24347_-\|BCCT-family-transporter	gnl\|CDD\|376718	pfam02028, BCCT, BCCT, betaine/carnitine/choline family transporter.	0
NZ_MWUW01000008.1\|WP_096543249.1\|27056_27401_-\|CRISPR-associated-endonuclease-Cas2	gnl\|CDD\|226043	COG3512, COG3512, CRISPR-associated protein, Cas2 homolog [Defense mechanisms].	7.95108e-29
NZ_MWUW01000008.1\|WP_173672491.1\|22010_22553_-\|alkaline-shock-response-membrane-anchor-protein-AmaP	gnl\|CDD\|380217	NF033218, anchor_AmaP, alkaline shock response membrane anchor protein AmaP. The founding member of this family, AmaP (Asp23 membrane anchoring protein), is related to Asp23 through part of its length, but includes a highly hydrophobic N-terminal region that should make it an integral membrane protein. Asp23 (alkaline shock protein of 23 kDa), described in PMID:7864904, is a cytosolic protein in Staphylococcus aureus, strongly induced by a pH shift from 7 to 10, and also recruited to the membrane. AmaP appears to be the partner protein with an integral membrane segment and the ability to anchor Asp23 to the membrane. This model was built to identify full-length homologs of AmaP, while excluding Asp23. Some but not all members of this family score above the cutoffs of Pfam model version PF03780.11, but full-length homologs of Asp23 score considerably higher. Asp23 family members previously were known as DUF322.	6.91232e-34
NZ_MWUW01000008.1\|WP_096543278.1\|36014_36818_-\|energy-coupling-factor-transporter-transmembrane-protein-EcfT	gnl\|CDD\|223692	COG0619, CbiQ, ABC-type cobalt transport system, permease component CbiQ and related transporters [Inorganic ion transport and metabolism].	5.57598e-43
NZ_MWUW01000008.1\|WP_096589032.1\|28294_32299_-\|type-II-CRISPR-RNA-guided-endonuclease-Cas9	gnl\|CDD\|273840	TIGR01865, conserved_hypothetical_protein, CRISPR subtype II/NMENI RNA-guided endonuclease Cas9/Csn1. CRISPR loci appear to be mobile elements with a wide host range. This model represents a protein found only in CRISPR-containing species, near other CRISPR-associated proteins (cas), as part of the NMENI subtype of CRISPR/Cas locus. The species range so far for this protein is animal pathogens and commensals only.	2.33265e-104
NZ_MWUW01000008.1\|WP_096543281.1\|32551_32857_-\|hypothetical-protein	gnl\|CDD\|225181	COG2272, PnbA, Carboxylesterase type B [Lipid metabolism].	0.00776549
NZ_MWUW01000008.1\|WP_096543241.1\|14880_16644_-\|siderophore-synthetase	gnl\|CDD\|226715	COG4264, RhbC, Siderophore synthetase component [Secondary metabolites biosynthesis, transport, and catabolism].	1.53127e-81
NZ_MWUW01000008.1\|WP_096543279.1\|35195_35999_-\|tRNA-pseudouridine(38-40)-synthase-TruA	gnl\|CDD\|234577	PRK00021, truA, tRNA pseudouridine(38-40) synthase TruA.	2.01205e-120
NZ_MWUW01000008.1\|WP_096589030.1\|17912_19871_+\|sialic-acid-synthase	gnl\|CDD\|226715	COG4264, RhbC, Siderophore synthetase component [Secondary metabolites biosynthesis, transport, and catabolism].	8.05495e-90
NZ_MWUW01000008.1\|WP_096543240.1\|13800_14877_-\|alanine-racemase	gnl\|CDD\|143490	cd06815, PLPDE_III_AR_like_1, Type III Pyridoxal 5-phosphate (PLP)-Dependent Enzyme Alanine Racemase-like 1. This subfamily is composed of uncharacterized bacterial proteins with similarity to bacterial alanine racemases (AR), which are fold type III PLP-dependent enzymes containing an N-terminal PLP-binding TIM-barrel domain and a C-terminal beta-sandwich domain. AR exists as homodimers with active sites that lie at the interface between the TIM barrel domain of one subunit and the beta-sandwich domain of the other subunit. It catalyzes the interconversion between L- and D-alanine, which is an essential component of the peptidoglycan layer of bacterial cell walls. Members of this subfamily may act as PLP-dependent enzymes.	2.12985e-118
NZ_MWUW01000008.1\|WP_014613243.1\|34137_34536_-\|30S-ribosomal-protein-S9	gnl\|CDD\|178888	PRK00132, rpsI, 30S ribosomal protein S9; Reviewed.	4.39067e-82
NZ_MWUW01000008.1\|WP_014613242.1\|34549_34987_-\|50S-ribosomal-protein-L13	gnl\|CDD\|181703	PRK09216, rplM, 50S ribosomal protein L13; Reviewed.	6.54958e-97

>NZ_MWUW01000008.1|WP_096543248.1|24403_25414_-|zinc-binding-alcohol-dehydrogenase-family-protein
MEAIIARQPFQLGEGQKFEKVSREVPTPGAHDILVKVHVTGVNPVDTKMRQAPLNGDTRVLGFDAAGVVEAVGDAVTDFKAGDRVYYSGSNQRDGSNQTYQLVNENYLAHMPKTLSFAEAAALPLTAITAYETLFDVFNISHNPQENVGKTLFIINGAGGVGSIATQIAKAYGLTVITTASRPETKAWSEKMGADVVLNHKEDLTKQFDDQNLARPNYIFCTYDTDHYYDVMIDLVQPRGHIATIVAFNHKQDLNALKQKSVTFTHEFMFARAIHGVDEWMYRKYLEDVTAKVDAGQYQTTLNAVLTGLTPENILKAHEMMEGQSHIGKLVIEIVE
>NZ_MWUW01000008.1|WP_096543247.1|22832_24347_-|BCCT-family-transporter
MKQTEKKKHVSLVFWISLLLCSLFVLVGAIFPKQMEQTTQLITTWIGQNFSWYYLLLLLAIFLICVYLLFSRYSQITLGEEGEEPEFSLKSWFAMLFSAGMGIGLVFWTTAEPISHAYTATPIHQAGTQAAIDDSLQFAFFHWGIHAWAVYAIVALAFAYFNFHKGYPGLVSATLTPLFGEKRMQGPLGKLLDILAIIATVTGVSATLGFGALQISEGLNFLFHIPANFTTQILIIIIATALFIWSTWSGLSKGIKVLSNINMILAFIVLVVVFIIGPTLYILNMFTNSLGNYIAHFFDMSLRLTINNPEKTQWIQNWTVFYWAWWISWAPFVGIFIARVSRGRTIKEFIVGVLFVPSFVCFIFFAVFGASAIYLQDTGIANIAGEAIETATFATLEHFPLGFVMSIVTLVVVMIFFVTSADSATYVLGMLSTNGDNYPGSWVKVSWGIMLAFFSLIMLLTGGTQAIQNLLIIAALPFSFVIIFMIWSLFKALAAERPRLSKRK
>NZ_MWUW01000008.1|WP_173672491.1|22010_22553_-|alkaline-shock-response-membrane-anchor-protein-AmaP
MTRIKNFILGLLIVVIVGFLLFMYVDYQPIKQYQNYFYQFNWFQPLLIGLAGLLILIGLILFFRAFKPTYRKPGLYKDYSDGHIYISRKSIENTVYDTLSKYDEIRQPNVVSKLYNKKKNSFIDITTDFIVPSESTHVPSLVEQVRLDVKESVERFSELHVKSVKVNVRDQKRDNGPRVV
>NZ_MWUW01000008.1|WP_019165072.1|21752_21998_-|DUF2273-domain-containing-protein
MSNENRNGQSERDSVEQFAKILQNYKWRIIGFFFFLIIAILFLTLGFWKTILIIVLCLIGIGVGYIKDRTQDFLNLLNRLS
>NZ_MWUW01000008.1|WP_096543245.1|21207_21699_-|Asp23/Gls24-family-envelope-stress-response-protein
MAVENNKAKESYNRETGVNENERQQREENQSQQFSNSLTFSDEVVEKIAGIAAREVRGILDMKGGFVDSISGSFSGGNNVTQGVSAEVGEKQAAIDLKVILEYGESAPKIFRKVTELIKEQVKHITGLDVVEVNMQVDDVMTRKEWQQKHEKNKDQNENKGLQ
>NZ_MWUW01000008.1|WP_096543244.1|19991_21137_+|MFS-transporter
MTIMRTLTFMLSIFIVGMVEMVVAGIMNLMSADLNISEAWIGQLVTIYAFTFALTGPILVKLTEKYSPKSVLLSAIAFFIIGNLMIALSPNFTILIIGRIISSAAAALIVVKILSTTVVLARPENRGKMLGIVYTGFSASNVFGVPIGTLIGDWAGWRFTFGLIILVGLIAGVLLTIYLPKEVKSSLQPTQNHTSRILDKGEVTKLLSITFILLTANSVAYIYINPLILSGGHNIQFVSLALLIIGIAGMLGTSMGGFFTDKLSFKTWLLVSGGAFVIVMLVLNQLWTASTILLSALFIWHIIQWSTNPAVQSGLIDQVEGDNSQVMSWNMSALNAGIGFGALLGGLIVSHIDLHATIYFAAGIGFIGFIIILMLKKKRVV
>NZ_MWUW01000008.1|WP_096589030.1|17912_19871_+|sialic-acid-synthase
MKTEQNHLSMIEKDFTKDEMENYQTVLQFDSNWATQFKRHVLVGRDKITARLMASLYRENLVGSYTHSTFVQAEQFAHSAIPSGELLMIHFTYGQLTLYARVIGHHAFNRVDVTGPFYWRTPDGEDRRVLHPNEVLDVIINKDTQYQGAAAEQFRDDMENSAAHMTLALSYQAQHPLKDASSLLDYIEAQDDPYLTSEQLVVEGHPLHPGAKLRKGMSAQETIDYSSEYQHAMPMQFILVHHDIARTMAQQGTYAEAVYHAFDGLKSVAQQAIGSHAPLEDYMPLIVHPWQYEHIIRTEYQHELDTKAIIPLDYAKSYYAGLSFRTLMPKKPDVSPHIKLSTNVHITGEIRTLSEQTTHNGPLMTNILTDISARDALFNTVSTSPVPEIAGAHFYQPTDSQPDEQERRSEQLGTLFRQNIYDMIDSDRLPVIPSSLVVTHPETARPLIIELVERFSQTNDSTTNMDVRAQWFKTYAASLIDYVVPLLVKYGIALEAHLQNTIAVFEPSTGAFSQMLIRDFEGLRIDAEQLAQMGYDTSHFHEKSRILTKSQTSVFNKAFYSTIQNHLGELVAALARFYDDAALENTLWSIVRQQIEQCFDKLEAADIDPERLAHMRDVFFNETIDYKCVTTMRLMDEAHEYTYVKVDNPLST
>NZ_MWUW01000008.1|WP_096543242.1|16627_17812_-|MFS-transporter
MAKWFFPSTFLLFLGNWIGQIALNWYAYQLNENPMDLALINFFRLAPIFILSLWAGSIADRYRRSTLIKITVTSSMVITGTLTACVLLMNDIPMLYLYIYACLRGCMSAIETPVRQAVLPDLSERLTVSKAVSYHSFLLNVCRSIGPAVAGFIIAAYDVPIAFVVQTICYALALVSSLPLKLAVVKPVKNKQFSLAVAMQYFKLHLQGRRIFMTSLLIMAMGYSYTTMLPILTDEVYPNSASVFGTAMTVSAIGGILATLTIPYVLQKFSTDNCYYLSSILFGIALLLLYPLGEIGLFLMIFFVGLFGQFARTTNRIYFQNDVEQESRGKILSVIMMDRGMIPLGAMLLSYFTELIGITTTFLVMGGATTVIAILGFLTNFKMNGGKTVHDTHA
>NZ_MWUW01000008.1|WP_096543241.1|14880_16644_-|siderophore-synthetase
MTLTHKLNADQNIQYRILIACMKEQLFPEKTQLQFKQNHVIIQYYQHVLAVQYETISTLYQLELSGPIVYRVGTADVTIETVEQLIEILGTHFGITFHDQLVEELLHSRLGLELSYQQLRQRERVMRHSLKFSRMPDTLNFIAWLNHMNGDEVFSVLGYTEGMVWEGHPSHPLTKTKLPLNAEEIQQYAPEFMKIIPLKMVLVRKNLLKSTTMTGEEDFVLRKMFPDKESQLRQWMQTYEGTLDDYRIMLVHPWQYANVITERFKTMIAQHDIIPTPFTVDSKATLSFRTMRLLHYPYHVKLPVNVQATSAVRTVSTVTTVDGPKLSYQLQDMLDIYPTLSVASEPYGAYIDVEADLARQFAMIVRHSPEVHTHDYTQLVTAVLTQVNPVDGQVVVDSLIEYLYGDINAETIEQFMRQYMEALIPPLIAYIQQYGIALEAHQQNTILQIDKENETLSFIVRDLGGSRIYPETLKQTVPDFEITNDSLVSDEIEAVIAKFQHAVIQNQLGTLIHHFHHVHQIDEATLYAIAAAEIEAAIDPTRPHADALKRMLFGDRMTVKALLNMRMEQKVKQYVQIDIKNPLEREL
>NZ_MWUW01000008.1|WP_096543240.1|13800_14877_-|alanine-racemase
MPQIHINLSKIKYNAMVLQHMLAEQGVRMIPVTKCMGGDTKVRQQFREMGFNTLAESRLTAMMSSEQQPTEHDMMIKGALPHEIHDVVRHSQISIQTELETIRALSDEAVAQGVKHHIYLMVDWKDGREGALTYEVVPLIKEIIRLKGVYLKGLSFNFMCFRQIPPTEEDIVYINYFIESVERDTGVKFKTISGGNSSMLTLAMYADLGRINELRIGEALFRGYETAYNMRLPYLFDNAVTLSGRILEIKPRLNLETRQSYMQALVDIGQLDTVISGIQPVDRQLKIVGHSSDTLMMNLGLADYYQIGDKIEFHLDYGALAHSMHMQHIPRSYDEDLGIEQLVSGFVDIKKNNFIKQY
>NZ_MWUW01000008.1|WP_096543249.1|27056_27401_-|CRISPR-associated-endonuclease-Cas2
MFFKEICVEVRYLRLILMFDLPVETSSERRAYRQFVKFLTNEGYVRLQYSVYCKLIFNSSTLNHQINKLKSIVPSNGAVQTLVVTENQFSNMKYLVGSPPKGQIGLSSNRVIEL
>NZ_MWUW01000008.1|WP_096589032.1|28294_32299_-|type-II-CRISPR-RNA-guided-endonuclease-Cas9
MAQKPYILSLDIGTGSVGYACMDKDFNILKYYDKDAIGVYLFDGAQTAQDRRQFRTARRRNNRRIKRLGLLQEILAPLVQNPNFYQFQRQFTWKNNNMDFKNKSLSEVLNFLGYAPKKYPTIYHLQKALLLKDEKFDPELIYVALYHLVKYRGHFLFNHLNVENLSDNDSMDDFVELIELYGSINHISLNLDYDKIKTIYEILQDNEMTKNDREKAIVKIEKALKHFSKMLLGLKFKEADLFSLADNAEELKEAKQSHSFTDNYEENLTSFLTVEQLELIERANKIYLALTLQDILNGNKSLAISKVAAYDKFKNELKLVKDIVYQADPTKAQFKKIFVSSKESLKQYEAIPNKENLSQLCLFDQYLIRPKDQYKKLMDELKKIIPQESPLYFEAQNDTLLKVLNTTDNASIPMQINLYEAETILRNQQKYHAEITDDMIEKVLSLIQFRIPYYVGPLVNDQTIAKFGWMSRKSNKPFKPWNFDEVVDRSKSATQFIRRMTNKCTYLMHEDVLPKNSLLYQEMELLNELNVIQVRLQTDPKSRKYRLTPQIKLFAIEHIFKKYKTVSHSKFLEIMLNSNHRETFMNHGEKLSIFGTQDDKKFVSKLSSYQDMTKIFGNIDDKRVQIEEIIQWITIFEDKKILVQKLKENYPELTSQQINQLKKLNYSGWGRLSERLLTHKYHDHSIIELLRSSDENFMEIITNDAYGFQNFIKEENQVQSNTIQYQDIANLATSPALKKGIWSTIKLVRELTSIFGEPEKIIMEFATEDQQKGKKQKSRKQLWDDNIKKNKLKSVDEYKYIIDVANKLNNEQLQQEKLWLYLSQNGKCMYSGQSIDLDALLSPNATKYYEVDHIIPRSFIKDDSIDNKVLVIKAMNQTKGDQVPLQFIQHSNEKIAYWKSLNKSGLISDNKLHKLMKPEFTAMDKEGFIQRQLVETRQISVHVRDFLIEEYPNSKVIPMKAKMVSEFRKKFEIPKIRQINDAHHAIDAYLNGVVYHGAQLAYPNVDLFDFNFKWEKVREKWKALGEFNTNQKARELFFFKKLEKMEVSQGERLISKIKLDMNHFKINYSKKLANTTQQFYNQMPESPKIAEIKYESNKSADAVYTSLKPHQTFVVAIKTATKKGKVKMQYQMIDYYVIDFYKFKNTDEKELALYLARRENKDEVLDAQIVYKLNKGDLLYLNNHPCYFVSSKEVINAKQFELTVEEQLSLYKVMHNKETSVEKLIKVYDLIAEKVIDDYHHYLDSELKVERVRTLFSESHQTHEDFIKALGELFKVVTASATRSEKIGSRKSGMGHRAFLGKGKDVKIAYTSISGLKTTKPKSLFKLAESRNEL
>NZ_MWUW01000008.1|WP_096543281.1|32551_32857_-|hypothetical-protein
MNAFKPHDHIAGELEKMRIAEFEQPTTALLQQFENQGSDVHFQIIDGGNDRPGAPHCFDLPLLFGNFEQWSNAPMLEGAEDQKLKAVSRQLQQHVAQWMRA
>NZ_MWUW01000008.1|WP_142302274.1|32868_33036_-|sce7725-family-protein
MYYPYLRGKQYELIAVRELIEKKLISKNVVPIIEPIKETATLRSTLETGIENNHG
>NZ_MWUW01000008.1|WP_096543280.1|33010_33883_-|sce7726-family-protein
MATINVLNKVFSKNVLRDLKREGESDLLNKAYYQIFNDDAPHLNNHKKIEQLYAVLKKNYRNEYFFKNTMLNKLLLGKHSLNTTVAMTELPIKRSKADFILINGKAIVYEIKTKLDALQRLNNQIMDYYTVFDHVEIIVDEAHLDKVKSRYLNTSVGISVLSKKNTISQKQKPSPNRQWLNHIAIYKVLRKEERKSIIKMFYGYSPEFDQFSEYQALLKMFQKIEIEPLYEQFIQLLKQRDTVKAYQNDFKNVPYELRFLTYFSQFRKNDYHNLNIALGGGNDVLSISKR
>NZ_MWUW01000008.1|WP_014613243.1|34137_34536_-|30S-ribosomal-protein-S9
MTLAQVEYRGTGRRKNSVARVRLVPGEGNIKVNDRDVREYLPFESLILDLNQPFDVTETKGNYDVLVNVHGGGFTGQAQAIRHGIARALLEADPEYRGSLKRAGLLTRDPRMKERKKPGLKKARRSPQFSKR
>NZ_MWUW01000008.1|WP_014613242.1|34549_34987_-|50S-ribosomal-protein-L13
MRQTFMANESNIERKWYVVDAEGQTLGRLSSEVAAILRGKNKVTYTPHVDTGDYVIIINASKIELTGNKENDKIYYRHSNHPGGLKSITAGELKRTNPERLLETSIKGMLPSSRLGEKQGKKLFVYGGAEHPHAAQQPENYELRG
>NZ_MWUW01000008.1|WP_096543279.1|35195_35999_-|tRNA-pseudouridine(38-40)-synthase-TruA
MQRVLVKISYNGAQFLGFQIQNQGRTVQGYFEKILKRMHQQHVRIHPSSRTDRGVHAKAQYFHFDTHLNITPERWQHAMNSALPDDIYVHDVSFIDQDFHCRYDCVGKRYRYSIYQADHRNPFWSQLKTFEPKALDIEAMQRAAKYFVGTHDFTTFCSQKTEVESKERTIYESRIEQTEDGIDFVITGSGFLYNMVRVIMAYLLEVGKGKRSADDVPNLLAQQDRNLVPHTAPAEGLYLEKIYLAPEQLTADFGDDIQIHYKKSTQI
>NZ_MWUW01000008.1|WP_096543278.1|36014_36818_-|energy-coupling-factor-transporter-transmembrane-protein-EcfT
MKDKLIIGRFLPLDTVIHRLDPRAKLIFVFLFIIIIFLSHDPLSYLWLALILLIITKLARIPLWFLIKGLLPVFFFLILTFMMHLFLTKGGTRLIEWGFITIDSNGIREGVLIMFRLGFIMIVSTILTLTTSPLNLTDAFDRLFKPLKYIKIPVAALSMMMSIALRFIPTLMEELDKIILSQKSRGSDISSGSLTARIKAFIPLLIPLFISAFQRAEELAIAMEVRGYDANAERTSYRILKWQLKDTLLIISLVPIAAVSLWLGHTF
>NZ_MWUW01000008.1|WP_096543277.1|36807_37674_-|energy-coupling-factor-transporter-ATPase
MIKFEEVSYTYQKNTPYEYQALNQVSTEFLPGRYYAIIGKTGSGKSTLIQHFNGLVKPTRGKVQVLDVTVNRKTKDKHLKSIRQRIGMVFQFPESQLFEETVEKEVLFGPRNYGLDLEEARGKALMLLGELGFDAEKVMQQSPFLLSGGQMRKIALAAILAMDPDILILDEPTAGLDPRSKAQVMALFKKLQLEEGKTIILVTHDMNDVAEFADEIKIMQGGTLIESTTPYDFFQRPDDVNALHLCLPDIVRLQRDVEAKLGIQFDKLSLTEASFVAMYEEWRQRHER

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Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity
NZ_MWUW01000008_1	1.12\|25890\|30\|NZ_MWUW01000008\|CRISPRCasFinder	25890-25919	30	NZ_KT373969	Staphylococcus pseudintermedius strain HR547/11 plasmid pKM01, complete sequence	29584-29613	0	1.0
NZ_MWUW01000008_1	1.17\|25891\|30\|NZ_MWUW01000008\|PILER-CR	25891-25920	30	NZ_KT373969	Staphylococcus pseudintermedius strain HR547/11 plasmid pKM01, complete sequence	29584-29613	0	1.0
NZ_MWUW01000008_1	1.9\|25695\|29\|NZ_MWUW01000008\|CRISPRCasFinder	25695-25723	29	MK075005	Staphylococcus phage phiSP119-2, partial genome	5963-5991	2	0.931
NZ_MWUW01000008_1	1.14\|25696\|29\|NZ_MWUW01000008\|PILER-CR	25696-25724	29	MK075005	Staphylococcus phage phiSP119-2, partial genome	5963-5991	2	0.931
NZ_MWUW01000008_1	1.13\|25956\|31\|NZ_MWUW01000008\|CRISPRCasFinder	25956-25986	31	MF417888	Uncultured Caudovirales phage clone 9S_3, partial genome	44874-44904	6	0.806
NZ_MWUW01000008_1	1.18\|25957\|31\|NZ_MWUW01000008\|PILER-CR	25957-25987	31	MF417888	Uncultured Caudovirales phage clone 9S_3, partial genome	44874-44904	6	0.806
NZ_MWUW01000008_1	1.3\|25695\|40\|NZ_MWUW01000008\|CRT	25695-25734	40	MK075005	Staphylococcus phage phiSP119-2, partial genome	5952-5991	7	0.825
NZ_MWUW01000008_1	1.6\|25890\|41\|NZ_MWUW01000008\|CRT	25890-25930	41	NZ_KT373969	Staphylococcus pseudintermedius strain HR547/11 plasmid pKM01, complete sequence	29584-29624	8	0.805

1. spacer 1.12|25890|30|NZ_MWUW01000008|CRISPRCasFinder matches to NZ_KT373969 (Staphylococcus pseudintermedius strain HR547/11 plasmid pKM01, complete sequence) position: , mismatch: 0, identity: 1.0

actatgtcagacctcaaaggaaagattctt	CRISPR spacer
actatgtcagacctcaaaggaaagattctt	Protospacer
******************************

2. spacer 1.17|25891|30|NZ_MWUW01000008|PILER-CR matches to NZ_KT373969 (Staphylococcus pseudintermedius strain HR547/11 plasmid pKM01, complete sequence) position: , mismatch: 0, identity: 1.0

actatgtcagacctcaaaggaaagattctt	CRISPR spacer
actatgtcagacctcaaaggaaagattctt	Protospacer
******************************

3. spacer 1.9|25695|29|NZ_MWUW01000008|CRISPRCasFinder matches to MK075005 (Staphylococcus phage phiSP119-2, partial genome) position: , mismatch: 2, identity: 0.931

gttacttaataaaatgtgccacgatttta	CRISPR spacer
attgcttaataaaatgtgccacgatttta	Protospacer
.**.*************************

4. spacer 1.14|25696|29|NZ_MWUW01000008|PILER-CR matches to MK075005 (Staphylococcus phage phiSP119-2, partial genome) position: , mismatch: 2, identity: 0.931

gttacttaataaaatgtgccacgatttta	CRISPR spacer
attgcttaataaaatgtgccacgatttta	Protospacer
.**.*************************

5. spacer 1.13|25956|31|NZ_MWUW01000008|CRISPRCasFinder matches to MF417888 (Uncultured Caudovirales phage clone 9S_3, partial genome) position: , mismatch: 6, identity: 0.806

tccgcttggtacttaaagcgaatccaccaca	CRISPR spacer
ggcgcttgatatttaaagcgaatccaccaat	Protospacer
  ******.**.*****************

6. spacer 1.18|25957|31|NZ_MWUW01000008|PILER-CR matches to MF417888 (Uncultured Caudovirales phage clone 9S_3, partial genome) position: , mismatch: 6, identity: 0.806

tccgcttggtacttaaagcgaatccaccaca	CRISPR spacer
ggcgcttgatatttaaagcgaatccaccaat	Protospacer
  ******.**.*****************

7. spacer 1.3|25695|40|NZ_MWUW01000008|CRT matches to MK075005 (Staphylococcus phage phiSP119-2, partial genome) position: , mismatch: 7, identity: 0.825

gttacttaataaaatgtgccacgattttagttttacttca--	CRISPR spacer
attgcttaataaaatgtgccacgatttta--tcaagttcatc	Protospacer
.**.*************************  *. * ****

8. spacer 1.6|25890|41|NZ_MWUW01000008|CRT matches to NZ_KT373969 (Staphylococcus pseudintermedius strain HR547/11 plasmid pKM01, complete sequence) position: , mismatch: 8, identity: 0.805

actatgtcagacctcaaaggaaagattcttgttttacttca	CRISPR spacer
actatgtcagacctcaaaggaaagattcttgaagaagatac	Protospacer
*******************************    *  *

Prophage detection

Region	Region Position	Protein_number	Hit_taxonomy	Key_proteins	Att_site	Prophage annotation

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

3. NZ_MWUW01000021

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

249 : 8803

Staphylococcus_phage(100.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_096589151.1\|249_669_-	transcriptional regulator	I1W651	Staphylococcus_phage	1.5e-48	71.9
WP_179841633.1\|668_827_-	hypothetical protein	A0A1J0MFM4	Staphylococcus_phage	1.5e-09	70.2
WP_096589153.1\|823_994_-	transcriptional regulator	A0A1J0MFF2	Staphylococcus_phage	1.1e-07	58.2
WP_096586317.1\|974_1139_-	DUF1381 domain-containing protein	A0A1J0MFI3	Staphylococcus_phage	1.3e-11	80.4
WP_051006009.1\|1135_1543_-	hypothetical protein	A0A0D3MVN6	Staphylococcus_phage	8.2e-52	75.2
WP_096589155.1\|1586_2126_-	dUTP pyrophosphatase	Q4ZD34	Staphylococcus_phage	1.6e-87	90.4
WP_019166762.1\|2118_2304_-	hypothetical protein	Q4ZD37	Staphylococcus_phage	1.2e-07	55.7
WP_019166763.1\|2296_2524_-	hypothetical protein	A0A1J0MFV6	Staphylococcus_phage	6.2e-09	44.0
WP_179841634.1\|2538_2700_-	hypothetical protein	A0A223LGT8	Staphylococcus_phage	1.5e-20	88.7
WP_179841635.1\|2716_2887_-	hypothetical protein	NA	NA	NA	NA
WP_096589177.1\|2887_3229_-	hypothetical protein	A0A1J0MFI0	Staphylococcus_phage	1.8e-44	77.0
WP_096586320.1\|3228_3627_-	hypothetical protein	A0A1J0MFF1	Staphylococcus_phage	1.8e-56	81.8
WP_096589156.1\|3626_3977_-	hypothetical protein	Q4ZD40	Staphylococcus_phage	9.5e-57	94.8
WP_096589157.1\|3973_4159_-	hypothetical protein	A0A1J0MFY5	Staphylococcus_phage	8.6e-25	95.1
WP_096589159.1\|4169_4415_-	hypothetical protein	A0A223LIA9	Staphylococcus_phage	8.2e-39	96.3
WP_096589161.1\|4415_4868_-	DUF3310 domain-containing protein	A0A1J0MFM3	Staphylococcus_phage	2.2e-61	74.5
WP_179841636.1\|5105_5267_-	hypothetical protein	NA	NA	NA	NA
WP_096589163.1\|5263_5584_-	hypothetical protein	A0A223LFZ4	Staphylococcus_phage	1.6e-47	89.6
WP_096589165.1\|5596_5815_-	hypothetical protein	A0A1J0MFH2	Staphylococcus_phage	3.9e-32	94.4
WP_096589167.1\|5807_6239_-	RusA family crossover junction endodeoxyribonuclease	A0A1J0MFP7	Staphylococcus_phage	3.0e-76	96.5
WP_096589169.1\|6239_6419_-	hypothetical protein	A0A1J0MFQ5	Staphylococcus_phage	5.1e-22	86.4
WP_096589171.1\|6412_7186_-	ATP-binding protein	A0A1J0MFK2	Staphylococcus_phage	9.9e-139	97.7
WP_096589173.1\|7195_7909_-	helix-turn-helix domain-containing protein	A0A1J0MGB2	Staphylococcus_phage	5.2e-102	86.1
WP_096589175.1\|7972_8176_+	hypothetical protein	NA	NA	NA	NA
WP_096589179.1\|8314_8803_-	hypothetical protein	A0A1J0MFX6	Staphylococcus_phage	1.8e-82	87.0

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

4. NZ_MWUW01000004

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

0 : 53179

Staphylococcus_phage(45.83%)

portal,tail,capsid,head,tRNA,terminase

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_142302265.1\|0_428_+\|terminase	terminase small subunit	Q4ZAI5	Staphylococcus_virus	2.7e-45	71.5
WP_096540533.1\|414_1680_+\|terminase	PBSX family phage terminase large subunit	Q4ZE37	Staphylococcus_virus	1.0e-185	73.1
WP_096540535.1\|1692_3165_+\|portal	phage portal protein	A0A1J0MFN1	Staphylococcus_phage	7.9e-161	59.2
WP_096540537.1\|3169_4135_+\|capsid	minor capsid protein	A0A1J0MFV1	Staphylococcus_phage	2.7e-101	57.6
WP_173672452.1\|4138_4306_+	hypothetical protein	NA	NA	NA	NA
WP_096540539.1\|4533_5118_+	DUF4355 domain-containing protein	A0A1J0MFB3	Staphylococcus_phage	1.2e-14	45.3
WP_096540541.1\|5137_6055_+\|capsid	capsid protein	Q4ZE33	Staphylococcus_virus	1.0e-121	72.3
WP_096540543.1\|6074_6335_+	hypothetical protein	A0A1W6JNX0	Staphylococcus_phage	5.7e-14	48.3
WP_096540545.1\|6338_6677_+\|head,tail	phage head-tail connector protein	A0A1W6JNR3	Staphylococcus_phage	7.6e-27	64.9
WP_096540547.1\|6673_6988_+\|head,tail	head-tail adaptor protein	A1KX32	Staphylococcus_virus	2.4e-14	40.8
WP_096540549.1\|6987_7332_+	HK97 gp10 family phage protein	A0A2H4J1L1	uncultured_Caudovirales_phage	4.4e-30	58.6
WP_096540551.1\|7343_7730_+	hypothetical protein	A0A1W6JNL2	Staphylococcus_phage	3.8e-30	50.4
WP_096588908.1\|7753_8554_+\|tail	phage major tail protein, TP901-1 family	A0A1W6JNU0	Staphylococcus_phage	2.6e-57	65.7
WP_096540555.1\|8623_9001_+\|tail	phage tail protein	A0A1W6JNK2	Staphylococcus_phage	6.7e-40	67.8
WP_096540952.1\|9045_9414_+	hypothetical protein	Q4ZE25	Staphylococcus_virus	1.5e-20	49.1
WP_096540557.1\|9395_13121_+\|tail	phage tail protein	A0A1W6JNM2	Staphylococcus_phage	3.7e-13	37.7
WP_096588910.1\|13131_14103_+\|tail	phage tail family protein	Q4ZE20	Staphylococcus_virus	4.9e-42	34.0
WP_096540561.1\|14114_16256_+	hypothetical protein	A0A2H4JH16	uncultured_Caudovirales_phage	2.5e-22	47.7
WP_096540563.1\|16271_18191_+\|tail	phage tail protein	A0A2P1JTV8	Anoxybacillus_phage	3.1e-64	38.0
WP_096540565.1\|18199_18472_+	hypothetical protein	A0A2H4J771	uncultured_Caudovirales_phage	5.0e-21	56.0
WP_096540567.1\|18541_18841_+	DUF2951 family protein	Q4ZD60	Staphylococcus_phage	5.3e-32	78.1
WP_096540568.1\|18980_19229_+	PTS mannose transporter subunit IID	Q4ZD59	Staphylococcus_phage	2.2e-36	91.5
WP_173672451.1\|19231_19381_+	hypothetical protein	NA	NA	NA	NA
WP_096540570.1\|19365_20214_+	SH3 domain-containing protein	A0A2H4IZS3	uncultured_Caudovirales_phage	1.5e-39	50.9
WP_096540955.1\|21053_21323_+	chorismate mutase	NA	NA	NA	NA
WP_170141200.1\|22242_22686_+	VOC family protein	NA	NA	NA	NA
WP_096540573.1\|22809_24114_-	potassium transporter KtrB	NA	NA	NA	NA
WP_103214038.1\|24496_24568_-	alpha-1/alpha-2 family phenol-soluble modulin	NA	NA	NA	NA
WP_096540577.1\|24999_25131_+	beta-class phenol-soluble modulin	NA	NA	NA	NA
WP_096540579.1\|25462_26149_+	YjjG family noncanonical pyrimidine nucleotidase	NA	NA	NA	NA
WP_019165670.1\|26348_26798_-	N-acetyltransferase	NA	NA	NA	NA
WP_096540581.1\|26929_28543_+	bacillithiol biosynthesis cysteine-adding enzyme BshC	NA	NA	NA	NA
WP_014614186.1\|28689_29121_+	division/cell wall cluster transcriptional repressor MraZ	NA	NA	NA	NA
WP_096540583.1\|29138_30074_+	16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH	NA	NA	NA	NA
WP_014614184.1\|30092_30479_+	cell division protein FtsL	NA	NA	NA	NA
WP_096540585.1\|30459_32613_+	penicillin-binding protein	NA	NA	NA	NA
WP_096540587.1\|32808_33774_+	phospho-N-acetylmuramoyl-pentapeptide- transferase	NA	NA	NA	NA
WP_096540589.1\|33774_35124_+	UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase	NA	NA	NA	NA
WP_096588914.1\|35139_36030_+	FtsQ-type POTRA domain-containing protein	NA	NA	NA	NA
WP_096540593.1\|36246_37647_+	cell division protein FtsA	NA	NA	NA	NA
WP_019165662.1\|37676_38849_+	cell division protein FtsZ	NA	NA	NA	NA
WP_173672450.1\|38995_39814_+	peptidoglycan editing factor PgeF	NA	NA	NA	NA
WP_096540597.1\|39814_40489_+	YggS family pyridoxal phosphate-dependent enzyme	NA	NA	NA	NA
WP_096540599.1\|40494_41055_+	cell division protein SepF	NA	NA	NA	NA
WP_014614173.1\|41066_41360_+	YggT family protein	NA	NA	NA	NA
WP_096540601.1\|41788_42670_-	GRP family sugar transporter	NA	NA	NA	NA
WP_096540603.1\|43027_43837_+	MurR/RpiR family transcriptional regulator	NA	NA	NA	NA
WP_096540605.1\|43947_44871_+	dihydrodipicolinate synthase family protein	NA	NA	NA	NA
WP_096540607.1\|44898_46440_+	sodium:solute symporter	A0A240F3J2	Aeromonas_phage	3.0e-30	26.3
WP_096540608.1\|46459_46915_+	YhcH/YjgK/YiaL family protein	NA	NA	NA	NA
WP_096540610.1\|46941_48078_+	Gfo/Idh/MocA family oxidoreductase	NA	NA	NA	NA
WP_096540612.1\|48846_49617_+	RNA-binding protein	NA	NA	NA	NA
WP_096540614.1\|49640_50231_+	DivIVA domain-containing protein	NA	NA	NA	NA
WP_096540616.1\|50431_53179_+\|tRNA	isoleucine--tRNA ligase	A0A1V0SJ93	Klosneuvirus	1.7e-87	27.4

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

5. NZ_MWUW01000001

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

59364 : 82927

Staphylococcus_phage(95.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_096542012.1\|59364_60768_+	o-succinylbenzoate--CoA ligase	A0A2H4PQM9	Staphylococcus_phage	8.3e-136	51.9
WP_096542014.1\|60770_61757_+	o-succinylbenzoate synthase	A0A2H4PQM0	Staphylococcus_phage	6.6e-79	48.2
WP_096542016.1\|61732_61984_-	membrane protein insertion efficiency factor YidD	A0A2H4PQM5	Staphylococcus_phage	3.5e-29	70.6
WP_096542018.1\|62038_62509_+	nucleoside triphosphatase YtkD	A0A2H4PQM4	Staphylococcus_phage	1.0e-37	52.6
WP_173672485.1\|62489_63266_+	prolyl oligopeptidase family serine peptidase	A0A2H4PQM6	Staphylococcus_phage	5.3e-84	60.8
WP_096542020.1\|63579_65253_+	hypothetical protein	NA	NA	NA	NA
WP_096542022.1\|65416_66997_-	phosphoenolpyruvate carboxykinase (ATP)	A0A2H4PQN1	Staphylococcus_phage	9.7e-242	76.2
WP_096542024.1\|67457_68657_+	methionine adenosyltransferase	A0A2H4PQS6	Staphylococcus_phage	1.4e-179	80.1
WP_096542026.1\|68744_69656_+	NERD domain-containing protein	A0A2H4PQQ8	Staphylococcus_phage	4.2e-88	68.3
WP_096542028.1\|69797_70634_+	aldo/keto reductase	A0A2H4PQR8	Staphylococcus_phage	1.6e-113	70.4
WP_096542030.1\|70791_71139_-	fluoride efflux transporter CrcB	NA	NA	NA	NA
WP_096542032.1\|71135_71501_-	fluoride efflux transporter CrcB	A0A2H4PQR0	Staphylococcus_phage	1.1e-26	51.7
WP_019165951.1\|71533_71836_-	hypothetical protein	NA	NA	NA	NA
WP_096588767.1\|71989_72700_+	transaldolase	M1PR54	Cyanophage	1.5e-21	39.5
WP_096542036.1\|72876_73326_+	hypothetical protein	A0A2H4PQT8	Staphylococcus_phage	2.9e-10	36.0
WP_096542038.1\|73328_73760_-	competence protein ComK	NA	NA	NA	NA
WP_164715592.1\|73762_74251_-	sigma-70 family RNA polymerase sigma factor	A0A2H4PQT5	Staphylococcus_phage	1.6e-17	35.4
WP_014613594.1\|74448_74673_+	hypothetical protein	NA	NA	NA	NA
WP_096542040.1\|74829_75693_+	glucosaminidase domain-containing protein	A0A1J0MFB0	Staphylococcus_phage	6.4e-38	41.2
WP_096542042.1\|76080_77649_+	FAD/NAD(P)-binding protein	A0A2H4PQX2	Staphylococcus_phage	2.6e-13	60.3
WP_096542044.1\|78103_79150_+	bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD	A0A2H4PQS8	Staphylococcus_phage	2.3e-106	54.9
WP_096542046.1\|79154_79784_+	riboflavin synthase	A0A2H4PQS5	Staphylococcus_phage	4.7e-62	58.6
WP_096542047.1\|79797_80979_+	3,4-dihydroxy-2-butanone-4-phosphate synthase	A0A2H4PQS2	Staphylococcus_phage	8.8e-155	67.4
WP_173672486.1\|80996_81461_+	6,7-dimethyl-8-ribityllumazine synthase	A0A2H4PQS3	Staphylococcus_phage	7.2e-52	63.6
WP_019166088.1\|81940_82927_-	proline dehydrogenase family protein	A0A2H4PQT6	Staphylococcus_phage	1.8e-121	66.1

DBSCAN-SWA_2

87049 : 94876

Staphylococcus_phage(100.0%)

tRNA

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_096542057.1\|87049_87613_-	class I SAM-dependent methyltransferase	A0A2H4PQV0	Staphylococcus_phage	1.3e-55	55.7
WP_096542059.1\|87609_88563_-	TIGR01212 family radical SAM protein	A0A2H4PQV5	Staphylococcus_phage	1.7e-63	79.9
WP_096542061.1\|88709_89891_+	MFS transporter	A0A2H4PQR6	Staphylococcus_phage	1.2e-132	61.0
WP_096542125.1\|90199_92614_+\|tRNA	leucine--tRNA ligase	A0A2H4PQS0	Staphylococcus_phage	0.0e+00	82.4
WP_096542063.1\|92640_92952_+	rhodanese-like domain-containing protein	A0A2H4PQR9	Staphylococcus_phage	1.9e-32	68.0
WP_096542065.1\|93619_94876_-	NAD(P)/FAD-dependent oxidoreductase	A0A2H4PQX1	Staphylococcus_phage	2.9e-31	69.5

DBSCAN-SWA_3

129112 : 138500

Staphylococcus_phage(75.0%)

capsid,portal

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_096542113.1\|129112_130708_-	phosphoglycerate dehydrogenase	A0A2H4PQU8	Staphylococcus_phage	2.7e-58	51.0
WP_096542115.1\|130697_131858_-	alanine--glyoxylate aminotransferase family protein	NA	NA	NA	NA
WP_096542117.1\|132018_132477_-	SACOL1771 family peroxiredoxin	NA	NA	NA	NA
WP_096588773.1\|132551_133298_+	glycerophosphodiester phosphodiesterase	A0A075KJS1	Lactobacillus_phage	1.7e-15	26.6
WP_096543358.1\|133846_134800_-	ornithine cyclodeaminase	NA	NA	NA	NA
WP_096543357.1\|135149_135344_+	LSM domain protein	A0A1J0MG79	Staphylococcus_phage	5.1e-12	56.2
WP_096543356.1\|135525_135720_+	LSM domain protein	A0A1J0MG79	Staphylococcus_phage	5.0e-15	62.5
WP_096543360.1\|135752_135881_+	hypothetical protein	A0A1J0MFV1	Staphylococcus_phage	8.1e-06	62.9
WP_096543355.1\|135859_136057_+	LSM domain protein	A0A1J0MG79	Staphylococcus_phage	1.9e-14	63.1
WP_096543354.1\|136393_136966_+\|portal	phage portal protein	A0A2P1JTZ3	Anoxybacillus_phage	2.1e-45	50.0
WP_096543353.1\|136949_138500_+\|capsid	minor capsid protein	A0A1J0MF61	Staphylococcus_phage	9.9e-106	56.1

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

6. NZ_MWUW01000022

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

270 : 8086

Staphylococcus_phage(94.12%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_096540528.1\|270_672_-	hypothetical protein	A0A0E3XBL2	Staphylococcus_phage	2.8e-60	83.5
WP_096540526.1\|671_842_-	transcriptional regulator	A0A1J0MFF2	Staphylococcus_phage	6.7e-08	60.0
WP_096540524.1\|857_1046_-	DUF1381 domain-containing protein	A0A223LFC9	Staphylococcus_phage	3.0e-25	98.4
WP_096540522.1\|1109_1649_-	dUTP pyrophosphatase	Q4ZD34	Staphylococcus_phage	3.6e-87	92.2
WP_019165890.1\|1700_2000_-	MazG-like family protein	I6SB84	Staphylococcus_virus	1.9e-45	88.9
WP_019165891.1\|1983_2142_-	hypothetical protein	NA	NA	NA	NA
WP_158224542.1\|2231_2399_-	hypothetical protein	NA	NA	NA	NA
WP_096540520.1\|2398_2644_-	hypothetical protein	A0A1J0MG61	Staphylococcus_phage	6.3e-23	71.1
WP_096540518.1\|2640_2826_-	hypothetical protein	A0A1J0MFY5	Staphylococcus_phage	3.3e-24	93.4
WP_096540516.1\|2829_3081_-	hypothetical protein	A0A223LIA9	Staphylococcus_phage	2.0e-40	97.6
WP_096540514.1\|3081_3534_-	DUF3310 domain-containing protein	A0A1J0MFM3	Staphylococcus_phage	1.7e-61	75.2
WP_096540513.1\|3702_4029_-	hypothetical protein	A0A223LFZ4	Staphylococcus_phage	1.5e-35	74.5
WP_096540949.1\|4041_4251_-	hypothetical protein	A0A1J0MFH2	Staphylococcus_phage	1.8e-26	82.6
WP_096540511.1\|4243_4672_-	RusA family crossover junction endodeoxyribonuclease	A0A223LGP3	Staphylococcus_phage	3.3e-67	89.1
WP_096540509.1\|4658_4877_-	hypothetical protein	A0A223LGV6	Staphylococcus_phage	9.5e-31	91.5
WP_096540507.1\|4873_6118_-	AAA family ATPase	A0A1J0MF36	Staphylococcus_phage	6.7e-230	98.1
WP_096540505.1\|6110_6458_-	hypothetical protein	A0A1J0MFD5	Staphylococcus_phage	3.0e-55	94.8
WP_096540503.1\|6462_7191_-	helix-turn-helix domain-containing protein	A0A1J0MG54	Staphylococcus_phage	6.5e-108	89.7
WP_096540501.1\|7255_7459_+	hypothetical protein	NA	NA	NA	NA
WP_096589181.1\|7597_8086_-	hypothetical protein	A0A1J0MFX6	Staphylococcus_phage	1.9e-87	92.6

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

7. NZ_MWUW01000002

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

0 : 52746

Staphylococcus_virus(53.12%)

tRNA,capsid,head,plate,terminase,integrase,holin,tail,portal

attL 24109:24123|attR 55298:55312

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_142302262.1\|0_419_+\|terminase	terminase small subunit	Q4ZAI5	Staphylococcus_virus	1.5e-53	75.4
WP_096588786.1\|402_1668_+\|terminase	PBSX family phage terminase large subunit	Q4ZC75	Staphylococcus_virus	4.2e-227	89.8
WP_096588788.1\|1668_3093_+\|portal	phage portal protein	Q4ZC74	Staphylococcus_virus	2.4e-231	82.3
WP_096588790.1\|3061_4009_+\|capsid	minor capsid protein	A0A2H4JC26	uncultured_Caudovirales_phage	6.0e-138	76.5
WP_096588792.1\|4114_4708_+	phage scaffolding protein	Q4ZBR6	Staphylococcus_phage	8.3e-45	76.0
WP_096588794.1\|4728_5571_+\|capsid	N4-gp56 family major capsid protein	Q4ZBR5	Staphylococcus_phage	7.2e-135	91.4
WP_096588796.1\|5587_5872_+	Rho termination factor N-terminal domain-containing protein	Q4ZCE7	Staphylococcus_virus	2.5e-23	60.9
WP_096588798.1\|5871_6168_+\|head,tail	phage head-tail adapter protein	Q4ZCE6	Staphylococcus_virus	6.4e-38	76.5
WP_096588800.1\|6169_6505_+\|head	phage head closure protein	Q4ZCE5	Staphylococcus_virus	1.8e-44	72.1
WP_096588802.1\|6491_6905_+	HK97 gp10 family phage protein	Q4ZCE4	Staphylococcus_virus	1.6e-71	92.0
WP_096588804.1\|6915_7338_+	DUF3168 domain-containing protein	Q4ZCE2	Staphylococcus_virus	7.4e-64	82.9
WP_096588806.1\|7342_7939_+\|tail	phage tail protein	Q4ZCE1	Staphylococcus_virus	1.4e-76	77.5
WP_096588808.1\|7998_8496_+	hypothetical protein	Q4ZCE0	Staphylococcus_virus	1.9e-71	80.6
WP_096588810.1\|8564_8849_+	hypothetical protein	Q4ZCD9	Staphylococcus_virus	1.8e-34	78.9
WP_096588812.1\|8848_12079_+\|terminase	terminase	Q4ZCD8	Staphylococcus_virus	1.3e-203	58.1
WP_096588814.1\|12096_13011_+\|tail	phage tail family protein	Q4ZCD7	Staphylococcus_virus	8.1e-132	72.7
WP_096588816.1\|13019_14522_+\|tail	phage tail protein	Q4ZCD5	Staphylococcus_virus	6.9e-205	70.2
WP_096588818.1\|14524_17182_+	peptidase G2	Q4ZCD4	Staphylococcus_virus	0.0e+00	74.7
WP_096588820.1\|17181_18681_+\|plate	BppU family phage baseplate upper protein	Q4ZCD3	Staphylococcus_virus	2.9e-102	45.1
WP_096588822.1\|18692_19103_+	hypothetical protein	A0A1W6JQ86	Staphylococcus_phage	3.5e-26	60.9
WP_096588824.1\|19104_19254_+	XkdX family protein	A0A1W6JQ64	Staphylococcus_phage	4.4e-11	72.1
WP_096588826.1\|19317_19662_+	hypothetical protein	NA	NA	NA	NA
WP_096588830.1\|20199_20826_+	hypothetical protein	A0A1W6JQ05	Staphylococcus_phage	7.4e-52	50.0
WP_096588832.1\|21055_22630_+	glucosaminidase domain-containing protein	Q4ZCC7	Staphylococcus_virus	3.0e-211	68.1
WP_179300050.1\|22968_23874_+	collagen-like protein	A0A2H4J3Z3	uncultured_Caudovirales_phage	3.9e-17	38.5
WP_096588836.1\|23921_24227_+\|holin	phage holin	A0A068A215	Staphylococcus_phage	5.4e-24	58.2
24109:24123	attL	NA	NA	NA	NA
WP_096588838.1\|24239_25628_+	N-acetylmuramoyl-L-alanine amidase	A0A1D6Z273	Staphylococcus_phage	4.5e-150	58.6
WP_096588840.1\|25844_26192_-	hypothetical protein	NA	NA	NA	NA
WP_096588842.1\|26321_26513_+	hypothetical protein	A0A2H4PQS9	Staphylococcus_phage	9.9e-16	67.7
WP_142302263.1\|26548_27061_+	hypothetical protein	A0A1W6JPV9	Staphylococcus_phage	4.1e-08	35.5
WP_096540346.1\|28195_28528_+	helix-turn-helix transcriptional regulator	NA	NA	NA	NA
WP_096540344.1\|28511_29117_+	DUF2812 domain-containing protein	NA	NA	NA	NA
WP_096588844.1\|29227_29962_-	trypsin-like peptidase domain-containing protein	NA	NA	NA	NA
WP_096540340.1\|30074_30809_-	trypsin-like peptidase domain-containing protein	NA	NA	NA	NA
WP_014614671.1\|31127_31463_+	DUF1450 domain-containing protein	NA	NA	NA	NA
WP_096540338.1\|32293_32773_+	GNAT family N-acetyltransferase	NA	NA	NA	NA
WP_019166271.1\|32795_34094_+	HD domain-containing protein	A0A1B1ISR1	uncultured_Mediterranean_phage	3.5e-27	32.7
WP_019166270.1\|34171_34687_+	YwhD family protein	NA	NA	NA	NA
WP_096540336.1\|34901_35312_+	DUF1934 family protein	NA	NA	NA	NA
WP_096540334.1\|35313_36972_+\|tRNA	arginine--tRNA ligase	A0A1V0SBV9	Catovirus	2.1e-13	20.4
WP_096540332.1\|37018_37690_-	twin-arginine translocase subunit TatC	A0A1B1IVR7	uncultured_Mediterranean_phage	6.6e-14	28.9
WP_172596508.1\|37718_37913_-	twin-arginine translocase TatA/TatE family subunit	NA	NA	NA	NA
WP_096540330.1\|38726_40439_-	FTR1 family protein	NA	NA	NA	NA
WP_096540328.1\|40419_41646_-	deferrochelatase/peroxidase EfeB	NA	NA	NA	NA
WP_096540325.1\|41642_42497_-	hypothetical protein	NA	NA	NA	NA
WP_096540324.1\|43013_43895_+	ABC transporter substrate-binding protein	NA	NA	NA	NA
WP_096540322.1\|43884_44835_+	iron ABC transporter permease	NA	NA	NA	NA
WP_096540320.1\|44891_45596_+	HAD family hydrolase	NA	NA	NA	NA
WP_096588846.1\|45595_46396_+	alpha/beta hydrolase	NA	NA	NA	NA
WP_096540316.1\|46487_46991_+	hypothetical protein	NA	NA	NA	NA
WP_096540314.1\|47079_47796_+	hypothetical protein	NA	NA	NA	NA
WP_014614657.1\|48005_48377_-	SarA family transcriptional regulator	NA	NA	NA	NA
WP_096540312.1\|49875_50805_-	DMT family transporter	NA	NA	NA	NA
WP_019166256.1\|51018_51288_+	hypothetical protein	NA	NA	NA	NA
WP_096540310.1\|51617_51833_-	DUF2922 domain-containing protein	NA	NA	NA	NA
WP_096540308.1\|51848_52052_-	DUF1659 domain-containing protein	NA	NA	NA	NA
WP_096540306.1\|52194_52746_+\|integrase	tyrosine-type recombinase/integrase	D2XR58	Bacillus_phage	1.7e-20	31.7
55298:55312	attR	NA	NA	NA	NA

Anti-CRISPR protein detection

Acr_ID: NZ_MWUW01000002.1|WP_096588840.1|25844_26192_-|hypothetical-protein

Click the colored protein region to show detailed information

Acr_ID: NZ_MWUW01000002.1|WP_142302263.1|26548_27061_+|hypothetical-protein

Click the colored protein region to show detailed information

Acr ID

Acr position

Acr size

Homology with known anti

Neighbor HTH/AcRanker

Neighbor Aca

In prophage

Protospacer in prophage

NZ_MWUW01000002.1|WP_096588840.1|25844_26192_-|hypothetical-protein

25844_26192_-

115 aa aa

AcrIIA19

hit_Aca_id	identity	query_coverage	hit_coverage
anti_CRISPR3638\|WP_100006909.1\|AcrIIA19\|II-A\|Verified\|Staphylococcus-pseudintermedius	0.8521739130434782	1	1.0

0-52746

NZ_MWUW01000002.1|WP_142302263.1|26548_27061_+|hypothetical-protein

26548_27061_+

170 aa aa

AcrIIA15

hit_Aca_id	identity	query_coverage	hit_coverage
anti_CRISPR3630\|WP_142302263.1\|AcrIIA15\|II-A\|Verified\|Staphylococcus-delphini	1.0	1	1.0

0-52746

8. NZ_MWUW01000020

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

7008 : 13675

Staphylococcus_phage(75.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_096589135.1\|7008_8394_-	recombinase family protein	Q4ZA74	Staphylococcus_virus	8.5e-149	56.8
WP_096589137.1\|8451_9270_-	DUF5067 domain-containing protein	A0A1W6JND4	Staphylococcus_phage	7.4e-52	59.6
WP_096589139.1\|9320_9995_-	LexA family transcriptional regulator	A0A1W6JPS3	Staphylococcus_phage	9.0e-120	94.6
WP_096589141.1\|10183_10423_+	helix-turn-helix transcriptional regulator	A0A2H4J3I3	uncultured_Caudovirales_phage	5.3e-35	96.2
WP_096589143.1\|10436_11213_+	Rha family transcriptional regulator	Q4ZC33	Staphylococcus_virus	7.6e-115	80.2
WP_096589145.1\|11224_11446_+	hypothetical protein	B5WZL5	Staphylococcus_phage	1.4e-13	56.7
WP_179841632.1\|11561_11738_+	hypothetical protein	A0A223LGE0	Staphylococcus_phage	2.6e-15	82.8
WP_096589147.1\|11819_12080_+	DUF1108 family protein	A0A223LGN1	Staphylococcus_phage	2.4e-36	86.0
WP_019168340.1\|12091_12346_+	hypothetical protein	A0A1J0MFA5	Staphylococcus_phage	6.3e-34	84.5
WP_096589149.1\|12338_12887_+	host-nuclease inhibitor Gam family protein	A0A0H3U480	Staphylococcus_phage	1.6e-50	55.3
WP_019168338.1\|12890_13535_+	ERF family protein	D2JGK1	Staphylococcus_phage	6.4e-107	93.3
WP_142302270.1\|13527_13675_+	single-stranded DNA-binding protein	A0A1J0MFL8	Staphylococcus_phage	1.1e-17	91.8

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage