Please upload a prokaryotic genome sequence file in FASTA or Multi-FASTA format or GenBank format (Just for sequences from the same genome if containing multiple contigs)

Upload a prokaryotic genome sequence file and set corresponding parameters

Predict CRISPR-Cas system using CRISPRCasFinder, CRT, PILER-CR

Predict novel class 2 effector protein

step1:screen orphan or unclear CRISPR arrays (spacer number >= 2, repeat length ranging from 18 to 45) in the whole genome

step2:retain those CRISPR arrays with repeats dissimilar with the collected repeats of known CRISPR type (identity < 0.9, coverage < 0.9)

step3:detect large proteins (protein size >= 500 aa) with unknown function within the 5 upstream and downstream proteins of the retained CRISPR arrays

step4:searching for homologs of the candidate proteins from step3 in the in-house protein database consisting of upstream and downstream proteins (protein size >= 500 aa) of CRISPR arrays (spacer number >= 2, repeat length ranging from 18 to 45)

step5:evaluate and validate candidates by filtering the proteins or homologs neighbored with the CRISPR arrays of known CRISPR type

Return results for candidate novel effector protein with CRISPR-Cas system annotation