CRISPRimmunity

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Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Target MGE spacer number	Prophage number	Anti-CRISPR protein number
NZ_SRYT01000040	Enterococcus faecalis strain NM58_B2-5 NODE_40_length_357_cov_3644.87, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_SRYT01000011	Enterococcus faecalis strain NM58_B2-5 NODE_11_length_66213_cov_779.568, whole genome shotgun sequence	0 crisprs	NA	0	1	0
NZ_SRYT01000029	Enterococcus faecalis strain NM58_B2-5 NODE_29_length_11223_cov_671.636, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_SRYT01000026	Enterococcus faecalis strain NM58_B2-5 NODE_26_length_18396_cov_711.691, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_SRYT01000014	Enterococcus faecalis strain NM58_B2-5 NODE_14_length_61463_cov_730.119, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_SRYT01000016	Enterococcus faecalis strain NM58_B2-5 NODE_16_length_47940_cov_752.659, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_SRYT01000019	Enterococcus faecalis strain NM58_B2-5 NODE_19_length_42535_cov_734.512, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_SRYT01000030	Enterococcus faecalis strain NM58_B2-5 NODE_30_length_2739_cov_5789.04, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_SRYT01000010	Enterococcus faecalis strain NM58_B2-5 NODE_10_length_68479_cov_739.851, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_SRYT01000006	Enterococcus faecalis strain NM58_B2-5 NODE_6_length_151733_cov_909.857, whole genome shotgun sequence	1 crisprs	NA	0	0	0
NZ_SRYT01000009	Enterococcus faecalis strain NM58_B2-5 NODE_9_length_69408_cov_790.384, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_SRYT01000033	Enterococcus faecalis strain NM58_B2-5 NODE_33_length_1251_cov_2253.23, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_SRYT01000024	Enterococcus faecalis strain NM58_B2-5 NODE_24_length_24747_cov_783.579, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_SRYT01000037	Enterococcus faecalis strain NM58_B2-5 NODE_37_length_745_cov_1691.67, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_SRYT01000023	Enterococcus faecalis strain NM58_B2-5 NODE_23_length_28630_cov_899.071, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_SRYT01000001	Enterococcus faecalis strain NM58_B2-5 NODE_1_length_786750_cov_691.081, whole genome shotgun sequence	2 crisprs	csn2,cas2,cas9,cas3,DinG,DEDDh,csa3	8	2	0
NZ_SRYT01000027	Enterococcus faecalis strain NM58_B2-5 NODE_27_length_15135_cov_673.009, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_SRYT01000003	Enterococcus faecalis strain NM58_B2-5 NODE_3_length_294056_cov_795.044, whole genome shotgun sequence	0 crisprs	NA	0	1	0
NZ_SRYT01000004	Enterococcus faecalis strain NM58_B2-5 NODE_4_length_176591_cov_742.995, whole genome shotgun sequence	0 crisprs	cas14j,cas3	0	1	0
NZ_SRYT01000036	Enterococcus faecalis strain NM58_B2-5 NODE_36_length_959_cov_1548.2, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_SRYT01000022	Enterococcus faecalis strain NM58_B2-5 NODE_22_length_35853_cov_965.83, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_SRYT01000035	Enterococcus faecalis strain NM58_B2-5 NODE_35_length_984_cov_1355.61, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_SRYT01000008	Enterococcus faecalis strain NM58_B2-5 NODE_8_length_110646_cov_820.327, whole genome shotgun sequence	0 crisprs	RT	0	0	0
NZ_SRYT01000005	Enterococcus faecalis strain NM58_B2-5 NODE_5_length_160076_cov_819.228, whole genome shotgun sequence	0 crisprs	cas3	0	0	0
NZ_SRYT01000007	Enterococcus faecalis strain NM58_B2-5 NODE_7_length_137438_cov_801.952, whole genome shotgun sequence	0 crisprs	csa3,WYL	0	0	0
NZ_SRYT01000018	Enterococcus faecalis strain NM58_B2-5 NODE_18_length_43883_cov_811.105, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_SRYT01000017	Enterococcus faecalis strain NM58_B2-5 NODE_17_length_47465_cov_837.108, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_SRYT01000002	Enterococcus faecalis strain NM58_B2-5 NODE_2_length_328556_cov_678.087, whole genome shotgun sequence	0 crisprs	cas3,DinG	0	1	0
NZ_SRYT01000041	Enterococcus faecalis strain NM58_B2-5 NODE_41_length_332_cov_617.942, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_SRYT01000012	Enterococcus faecalis strain NM58_B2-5 NODE_12_length_63219_cov_780.098, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_SRYT01000034	Enterococcus faecalis strain NM58_B2-5 NODE_34_length_1131_cov_464.891, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_SRYT01000032	Enterococcus faecalis strain NM58_B2-5 NODE_32_length_1313_cov_5141.42, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_SRYT01000015	Enterococcus faecalis strain NM58_B2-5 NODE_15_length_54908_cov_835.406, whole genome shotgun sequence	1 crisprs	NA	2	0	2
NZ_SRYT01000021	Enterococcus faecalis strain NM58_B2-5 NODE_21_length_36090_cov_917.867, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_SRYT01000020	Enterococcus faecalis strain NM58_B2-5 NODE_20_length_41806_cov_706.304, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_SRYT01000031	Enterococcus faecalis strain NM58_B2-5 NODE_31_length_1986_cov_1338.75, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_SRYT01000039	Enterococcus faecalis strain NM58_B2-5 NODE_39_length_526_cov_3364.51, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_SRYT01000013	Enterococcus faecalis strain NM58_B2-5 NODE_13_length_61789_cov_819.542, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_SRYT01000025	Enterococcus faecalis strain NM58_B2-5 NODE_25_length_18699_cov_592.131, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_SRYT01000038	Enterococcus faecalis strain NM58_B2-5 NODE_38_length_542_cov_5780.89, whole genome shotgun sequence	0 crisprs	NA	0	0	0
NZ_SRYT01000028	Enterococcus faecalis strain NM58_B2-5 NODE_28_length_11655_cov_904.538, whole genome shotgun sequence	0 crisprs	NA	0	0	0

Results visualization

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CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

39951 : 50865

Streptococcus_phage(50.0%)

The bacterium proteins that are colored denote the protein is present at specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin' and 'tRNA')

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_002356382.1\|39951_40719_-	ABC transporter ATP-binding protein	G9BWD6	Planktothrix_phage	4.4e-30	35.2
WP_002385686.1\|41124_43308_+	anaerobic ribonucleoside-triphosphate reductase	A0A0C5KKX3	Enterococcus_phage	3.1e-286	66.8
WP_002356379.1\|43340_43937_+	anaerobic ribonucleoside-triphosphate reductase activating protein	A0A0C5K925	Enterococcus_phage	6.0e-51	56.4
WP_002362328.1\|43981_45100_-	DNA polymerase IV	D0R0A3	Streptococcus_phage	4.2e-13	29.1
WP_002356377.1\|45225_45984_-	iron export ABC transporter permease subunit FetB	NA	NA	NA	NA
WP_002356375.1\|45967_46630_-	ATP-binding cassette domain-containing protein	G9BWD6	Planktothrix_phage	2.0e-15	32.3
WP_002359438.1\|46644_47517_-	16S rRNA (cytidine(1402)-2'-O)-methyltransferase	M1PLC5	Streptococcus_phage	1.1e-80	57.2
WP_002356372.1\|47629_47977_-	DNA replication initiation control protein YabA	NA	NA	NA	NA
WP_002356371.1\|47969_48809_-	stage 0 sporulation family protein	NA	NA	NA	NA
WP_002389791.1\|48925_49876_-	DNA polymerase III subunit delta'	M1NSC1	Streptococcus_phage	6.2e-34	33.0
WP_002356367.1\|49877_50207_-	hypothetical protein	NA	NA	NA	NA
WP_002356366.1\|50226_50865_-	dTMP kinase	M1PSC7	Streptococcus_phage	2.1e-54	53.8

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

2. NZ_SRYT01000015

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CRISPR-Cas detection and classification

Crispr_ID: NZ_SRYT01000015_1

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

NZ_SRYT01000015_1

5371-5502

Orphan

Consensus_repeat	Method
GTTGGTTTTTCCCACTTTCGAACA	CRISPRCasFinder

2 spacers

The CRISPR arrays of NZ_SRYT01000015_1

>merge|NZ_SRYT01000015|1|5371-5502|CRISPRCasFinder
GTTGGTTTTTCCCACTTTCGAACAAAGTACTGGTATTATTGGATTCTTCTGGACGTTGGTTTTTCCCACTTTCGAAAGAAACGCCGATTTTATCATGTTTATCCGAAGGTTGGTTTTTCCCACTTTCGGGAA

>NZ_SRYT01000015|1|1|5371-5502|CRISPRCasFinder
GTTGGTTTTTCCCACTTTCGAACA	AAGTACTGGTATTATTGGATTCTTCTGGAC
GTTGGTTTTTCCCACTTTCGAAAG	AAACGCCGATTTTATCATGTTTATCCGAAG
GTTGGTTTTTCCCACTTTCGGGAA

Protein	Signature genes	Signature genes Name	Protein_function
NZ_SRYT01000015.1\|WP_002360663.1\|6185_6542_-\|hypothetical-protein	unknown	unknown	unknown
NZ_SRYT01000015.1\|WP_002393766.1\|10573_10876_-\|hypothetical-protein	unknown	unknown	unknown
NZ_SRYT01000015.1\|WP_002360665.1\|7570_7867_-\|replication-control-protein-PrgN	unknown	unknown	unknown
NZ_SRYT01000015.1\|WP_002360664.1\|6534_7317_-\|ParA-family-protein	unknown	unknown	gnl\|CDD\|224113
NZ_SRYT01000015.1\|WP_087548826.1\|8814_10146_-\|Y-family-DNA-polymerase	unknown	unknown	gnl\|CDD\|176454
NZ_SRYT01000015.1\|WP_002399367.1\|10887_11097_-\|hypothetical-protein	unknown	unknown	unknown
NZ_SRYT01000015.1\|WP_002366016.1\|1957_3112_-\|TraB/GumN-family-protein	unknown	unknown	gnl\|CDD\|224828
NZ_SRYT01000015.1\|WP_010715978.1\|3122_4760_-\|peptide-pheromone-binding-protein-TraC	unknown	unknown	gnl\|CDD\|173869
NZ_SRYT01000015.1\|WP_002366018.1\|971_1925_+\|helix-turn-helix-transcriptional-regulator	unknown	unknown	gnl\|CDD\|197775
NZ_SRYT01000015.1\|WP_002399364.1\|8467_8818_-\|hypothetical-protein	unknown	unknown	unknown
NZ_SRYT01000015.1\|WP_153829630.1\|8106_8196_-\|type-I-toxin-antitoxin-system-Fst-family-toxin	unknown	unknown	unknown
NZ_SRYT01000015.1\|WP_025190469.1\|11375_11690_-\|hypothetical-protein	unknown	unknown	unknown
NZ_SRYT01000015.1\|WP_002414746.1\|11157_11382_-\|hypothetical-protein	unknown	unknown	unknown

Protein	Function_ID	Function_description	E-value
NZ_SRYT01000015.1\|WP_002366016.1\|1957_3112_-\|TraB/GumN-family-protein	gnl\|CDD\|224828	COG1916, COG1916, Uncharacterized homolog of PrgY (pheromone shutdown protein) [Function unknown].	2.7588e-92
NZ_SRYT01000015.1\|WP_010715978.1\|3122_4760_-\|peptide-pheromone-binding-protein-TraC	gnl\|CDD\|173869	cd08504, PBP2_OppA, The substrate-binding component of an ABC-type oligopetide import system contains the type 2 periplasmic binding fold. This family represents the periplasmic substrate-binding component of an ATP-binding cassette (ABC)-type oligopeptide transport system comprised of 5 subunits. The transport system OppABCDEF contains two homologous integral membrane proteins OppB and OppF that form the translocation pore; two homologous nucleotide-binding domains OppD and OppF that drive the transport process through binding and hydrolysis of ATP; and the substrate-binding protein or receptor OppA that determines the substrate specificity of the transport system. The dipeptide (DppA) and oligopeptide (OppA) binding proteins differ in several ways. The DppA binds dipeptides and some tripeptides and is involved in chemotaxis toward dipeptides, whereas the OppA binds peptides of a wide range of lengths (2-35 amino acid residues) and plays a role in recycling of cell wall peptides, which precludes any involvement in chemotaxis. Most of other periplasmic binding proteins are comprised of only two globular subdomains corresponding to domains I and III of the dipeptide/oligopeptide binding proteins. The structural topology of these domains is most similar to that of the type 2 periplasmic binding proteins (PBP2), which are responsible for the uptake of a variety of substrates such as phosphate, sulfate, polysaccharides, lysine/arginine/ornithine, and histidine. The PBP2 bind their ligand in the cleft between these domains in a manner resembling a Venus flytrap. After binding their specific ligand with high affinity, they can interact with a cognate membrane transport complex comprised of two integral membrane domains and two cytoplasmically located ATPase domains. This interaction triggers the ligand translocation across the cytoplasmic membrane energized by ATP hydrolysis. Besides transport proteins, the PBP2 superfamily includes the ligand-binding domains from ionotropic glutamate receptors, LysR-type transcriptional regulators, and unorthodox sensor proteins involved in signal transduction.	0
NZ_SRYT01000015.1\|WP_002360664.1\|6534_7317_-\|ParA-family-protein	gnl\|CDD\|224113	COG1192, Soj, ATPases involved in chromosome partitioning [Cell division and chromosome partitioning].	2.41703e-71
NZ_SRYT01000015.1\|WP_087548826.1\|8814_10146_-\|Y-family-DNA-polymerase	gnl\|CDD\|176454	cd01700, PolY_Pol_V_umuC, umuC subunit of DNA Polymerase V. umuC subunit of Pol V. Pol V is a bacterial translesion synthesis (TLS) polymerase that consists of the heterotrimer of one umuC and two umuD subunits. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Pol V, RecA, single stranded DNA-binding protein, beta sliding clamp, and gamma clamp loading complex are responsible for inducing the SOS response in bacteria to repair UV-induced DNA damage.	3.00216e-136
NZ_SRYT01000015.1\|WP_002366018.1\|971_1925_+\|helix-turn-helix-transcriptional-regulator	gnl\|CDD\|197775	smart00530, HTH_XRE, Helix-turn-helix XRE-family like proteins.	1.69683e-05

>NZ_SRYT01000015.1|WP_010715978.1|3122_4760_-|peptide-pheromone-binding-protein-TraC
MKKYKKFCFLGIGLLPLVLASCGTNTATKDSQDATEKKVEQVATLTAGTPVQSLDPATAVDQTSITLLANVMEGLYRLDEKNQPQPAIAAGQPKVSNNGKTYTIVIRDGAKWSDGTQITASDFVAAWQRVVDPKTASPNVELFSAIKNAKEIASGKQAKDTLAVKSIGEKTLEIELVEPTPYFTDLLSLTAYYPVQQKAIKEYGKDYGVSQKAIVTNGAFNLTNLEGVGTSDKWTISKNKEYWDQKDVSMDKINFQVVKEINTGINLYNDGQLDEAPLAGEYAKQYKKDKEYSTTLMANTMFLEMNQTGENKLLQNKNVRKAISYAIDRESLVKKLLDNGSVASVGVVPKEMAFNPVNKKDFANEKLVEFNKKQAEEYWDKAKKEIDLSKNTSLDLLVSDGEFEKKAGEFLQGQLQDSLEGLKVNVTPIPANVFMERLTKKDFTLSLSGWQADYADPISFLANFETNSPMNHGGYSNKNYDELLKDSSSKRWQELKKAEKLLINDMGVVPIFQVGTAKLEKSKIKNVLMHSIGAKYDYKKMRIEK
>NZ_SRYT01000015.1|WP_002366016.1|1957_3112_-|TraB/GumN-family-protein
MELVRRVFIKDKEIILVGTAHISSESAELVKNIIEEENPDVVCLEWDQSRYDKYLNPDEWSDTDIVQVIKQKRLTVLISSVIYKLIQKHLAKINDSVAGAEFFEAVSSAEKVGAKIALVDRDSQVTFKRFWRLIPLRKKALLPYAFGSVLEGAEESEEEMLKLLNSENFEPIFEQLQEAYPELWKVLLVERDQYLAQKISDQEAGKIVVVIGQAHLRGVEKNLKSNQQINIKELETLPPKLWSTKVLESIIPLIIIGLLIYSFFLGVDVGKDQIMRWGIWNGGLSALFTLLALAHPLTIVTSLVFAPLATLLPMVSVGVFSAIVEATLRKPKVRDFQTMDEDIKSLKKIYKNRVLRVFLVFVLASLGGMIGNFVGGLEMFRHLI
>NZ_SRYT01000015.1|WP_002366018.1|971_1925_+|helix-turn-helix-transcriptional-regulator
MFKIGSVLKQIRQELNYHQIDLYSGIMSKSVYIKVEADSRPISVEELSKFSERLGVNFFEILNRAGMNTKSVNETGKEKLLISKIFTNPDLFDKNFQRIEPKRLTSLQYFSIYLGYISIAHHYNIEVPTFNKTITSDLKHLYDKRTTFFGIDYEIVSNLLNVLPYEEVSSIIKPMYPIVDSFGKDYDLTIQTVLKNALTISIMNRNLKEAQYYINQFEHLKTIKNISINGYYDLEINYLKQIYQFLTDKNIDSYLNAVNIINIFKIIGKEDIHRSLVEELTKISAKEKFTPPKEVTMYYENYVAIENNPIPEIKEQS
>NZ_SRYT01000015.1|WP_002360663.1|6185_6542_-|hypothetical-protein
MSNNRGKSAAERLLGAGKEDTNPTKATTSNEKELKTTHEHIVMNKKNIEKLIKQQGNSKRNADSIRINPILANALKYWTSIGEPEKSKPDVIEETLLKSIPEEYLIKGYEMAKKQNKI
>NZ_SRYT01000015.1|WP_002360664.1|6534_7317_-|ParA-family-protein
MARIISVANQKGGVSKTTTSINLGAALHFTFNKSVLVIDMDPQGNATDNFGFDIDGTNVPTIYEVLKDEASITEAILNYKGIDVIPADIALSSAEREFTQVGSEHRLKRVLQPIEENYDYIIIDCPPSLGILTVNAFTVSDEIIIPVEAAYFSLKGLVKLNETIETVKEYTNERLIIRGVLFTKYNERFNISKEMNKTANQISDALNVNIFDTYIRRTITVDEAQAAGSDLISFNKTSTAEDDYIQFTKEFLEKVGDLNE
>NZ_SRYT01000015.1|WP_002360665.1|7570_7867_-|replication-control-protein-PrgN
MPRKTFVYPHPINAYIIKQLGITVEEFCELHAFSQGTVSSWITRNKKIETLPISFIYSLSLSASQTMDQVYSDLLKLQDDYLLHLERHRRTKKIIDEE
>NZ_SRYT01000015.1|WP_153829630.1|8106_8196_-|type-I-toxin-antitoxin-system-Fst-family-toxin
MSLVIAPIFVGLVLEMISRVLDEEDDNRK
>NZ_SRYT01000015.1|WP_002399364.1|8467_8818_-|hypothetical-protein
MKQNRKEFSSYFSRSIKQNKPLYLLLMSSETNPFPRPVIGTFRGYAEENKIIIGEDSYSIEEIDFVCMYRHSSRIKQEERVNSNPFQGLVNPSLAKKEQKKLDEYFSDWGFRNEGI
>NZ_SRYT01000015.1|WP_087548826.1|8814_10146_-|Y-family-DNA-polymerase
MNLTFDYTKEPSRDVLCIDVKSFYASVECVERGLDPLKTMLVVMSNSENSGGLVLAASPMAKKVLGISNVTRKNEVPDHPNLIIVSPRMKLYMKKNQEINNLYKRFVSDEDHSVFSVDESFLDVTASLTYFKCDTAYKLAKIIQRVIYNHMGLYVTIGIGENPLLAKLALDNEAKNAPGFVAEWRYEDVPKKVWPISPLTEFCGIGNRMAARLKKLGIRSIYDLAHIEPYMLKERFGIMGLQLYAHSWGIDRSFLGQKAAGRPTEKSFGNSQVLPKDYANKEQIKLVLKELSDQVASRLRMASCQTTCVSLFVGYSKGQTDKYGQNGWRRQMKVEPSNNTKVLTEHVLRLFEENYAPGVDVRNLGVSYGRLVWNKNLQLDLFSVPEEQIHETDMYFLIDKIRQKFGFKALIHASSLMEGATAISRASLVGGHAGGTVGLGTTK
>NZ_SRYT01000015.1|WP_002393766.1|10573_10876_-|hypothetical-protein
MPVNKNGLPFYLDRKLDVIRFGVDPDNHVKEQRPFKSLVKTENPNKKERFIEKESESLTQLGYKIVVDTVTGVNYLESPRGGITPLLDETGKVCIDKISK
>NZ_SRYT01000015.1|WP_002399367.1|10887_11097_-|hypothetical-protein
MNFGYLNFGTKDAVLVIAIFIILILTVLFRWSALEFVFSSGAVGIAFFAGNMKQDKRIADRQNKKSEKQ
>NZ_SRYT01000015.1|WP_002414746.1|11157_11382_-|hypothetical-protein
MVVRKTYDHWGIEINTWNKSNIVTFIDCDCGQLAKRELGKYNQFKCDSCNKEYKLYQGNYIAIDEKTNEVAQND
>NZ_SRYT01000015.1|WP_025190469.1|11375_11690_-|hypothetical-protein
MRNNSGVVIMENREKIIQLLKNPLVTGYGIEIMSNGRLYSANFQRYKNRVKKEKNPLIIFESMTAKVEQVFLELAEEVIRTNPKTKQEFKDMIKEYSYKEGNKW

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Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity
NZ_SRYT01000015_1	1.1\|5395\|30\|NZ_SRYT01000015\|CRISPRCasFinder	5395-5424	30	NZ_KY513281	Enterococcus faecalis strain 6742 plasmid p6742_2, complete sequence	5073-5102	0	1.0
NZ_SRYT01000015_1	1.1\|5395\|30\|NZ_SRYT01000015\|CRISPRCasFinder	5395-5424	30	NC_004671	Enterococcus faecalis V583 plasmid pTEF2, complete sequence	602-631	0	1.0
NZ_SRYT01000015_1	1.1\|5395\|30\|NZ_SRYT01000015\|CRISPRCasFinder	5395-5424	30	NZ_CP015885	Enterococcus faecalis strain sorialis plasmid Efsorialis-p2, complete sequence	515-544	0	1.0
NZ_SRYT01000015_1	1.1\|5395\|30\|NZ_SRYT01000015\|CRISPRCasFinder	5395-5424	30	NZ_CP040897	Enterococcus faecalis strain HA-1 plasmid punnamed	33709-33738	0	1.0
NZ_SRYT01000015_1	1.1\|5395\|30\|NZ_SRYT01000015\|CRISPRCasFinder	5395-5424	30	CP002494	Enterococcus faecalis 62 plasmid EF62pC, complete sequence	7643-7672	0	1.0
NZ_SRYT01000015_1	1.1\|5395\|30\|NZ_SRYT01000015\|CRISPRCasFinder	5395-5424	30	MH830362	Enterococcus faecalis plasmid p4, complete sequence	66171-66200	0	1.0
NZ_SRYT01000015_1	1.1\|5395\|30\|NZ_SRYT01000015\|CRISPRCasFinder	5395-5424	30	NZ_CP031028	Enterococcus faecalis strain TY1 isolate flatfish plasmid pDEF-1, complete sequence	12176-12205	0	1.0
NZ_SRYT01000015_1	1.1\|5395\|30\|NZ_SRYT01000015\|CRISPRCasFinder	5395-5424	30	NZ_CP046109	Enterococcus faecalis strain 133170041-3 plasmid pAD1, complete sequence	503-532	0	1.0
NZ_SRYT01000015_1	1.1\|5395\|30\|NZ_SRYT01000015\|CRISPRCasFinder	5395-5424	30	NZ_CP019514	Enterococcus faecalis strain CLB21560 plasmid pB, complete sequence	60906-60935	0	1.0
NZ_SRYT01000015_1	1.1\|5395\|30\|NZ_SRYT01000015\|CRISPRCasFinder	5395-5424	30	NZ_CP036248	Enterococcus faecalis R712 plasmid pR712_02, complete sequence	92754-92783	0	1.0
NZ_SRYT01000015_1	1.1\|5395\|30\|NZ_SRYT01000015\|CRISPRCasFinder	5395-5424	30	NZ_CP049777	Enterococcus faecalis strain ES-1 plasmid unnamed2, complete sequence	503-532	0	1.0
NZ_SRYT01000015_1	1.1\|5395\|30\|NZ_SRYT01000015\|CRISPRCasFinder	5395-5424	30	NZ_CP028837	Enterococcus faecalis strain FC plasmid unnamed2, complete sequence	5526-5555	0	1.0
NZ_SRYT01000015_1	1.1\|5395\|30\|NZ_SRYT01000015\|CRISPRCasFinder	5395-5424	30	NZ_CP030046	Enterococcus faecalis strain C54 plasmid pC54, complete sequence	43889-43918	0	1.0
NZ_SRYT01000015_1	1.1\|5395\|30\|NZ_SRYT01000015\|CRISPRCasFinder	5395-5424	30	NC_011642	Enterococcus faecalis plasmid pMG2200, complete sequence	83505-83534	0	1.0
NZ_SRYT01000015_1	1.1\|5395\|30\|NZ_SRYT01000015\|CRISPRCasFinder	5395-5424	30	LC499744	Enterococcus faecalis S7316 plasmid pS7316optrA DNA, complete sequence	9536-9565	0	1.0
NZ_SRYT01000015_1	1.1\|5395\|30\|NZ_SRYT01000015\|CRISPRCasFinder	5395-5424	30	NZ_CP028284	Enterococcus faecalis strain FDAARGOS_324 plasmid unnamed2, complete sequence	4944-4973	0	1.0
NZ_SRYT01000015_1	1.1\|5395\|30\|NZ_SRYT01000015\|CRISPRCasFinder	5395-5424	30	NZ_CP030043	Enterococcus faecalis strain C25 plasmid pC25-1, complete sequence	827-856	0	1.0
NZ_SRYT01000015_1	1.2\|5449\|30\|NZ_SRYT01000015\|CRISPRCasFinder	5449-5478	30	NC_004671	Enterococcus faecalis V583 plasmid pTEF2, complete sequence	548-577	0	1.0
NZ_SRYT01000015_1	1.2\|5449\|30\|NZ_SRYT01000015\|CRISPRCasFinder	5449-5478	30	NZ_CP015885	Enterococcus faecalis strain sorialis plasmid Efsorialis-p2, complete sequence	461-490	0	1.0
NZ_SRYT01000015_1	1.2\|5449\|30\|NZ_SRYT01000015\|CRISPRCasFinder	5449-5478	30	NZ_CP040897	Enterococcus faecalis strain HA-1 plasmid punnamed	33655-33684	0	1.0
NZ_SRYT01000015_1	1.2\|5449\|30\|NZ_SRYT01000015\|CRISPRCasFinder	5449-5478	30	NZ_CP046109	Enterococcus faecalis strain 133170041-3 plasmid pAD1, complete sequence	449-478	0	1.0
NZ_SRYT01000015_1	1.2\|5449\|30\|NZ_SRYT01000015\|CRISPRCasFinder	5449-5478	30	NZ_CP028837	Enterococcus faecalis strain FC plasmid unnamed2, complete sequence	5472-5501	0	1.0
NZ_SRYT01000015_1	1.2\|5449\|30\|NZ_SRYT01000015\|CRISPRCasFinder	5449-5478	30	NZ_CP030046	Enterococcus faecalis strain C54 plasmid pC54, complete sequence	43943-43972	0	1.0
NZ_SRYT01000015_1	1.2\|5449\|30\|NZ_SRYT01000015\|CRISPRCasFinder	5449-5478	30	NC_011642	Enterococcus faecalis plasmid pMG2200, complete sequence	83451-83480	0	1.0
NZ_SRYT01000015_1	1.2\|5449\|30\|NZ_SRYT01000015\|CRISPRCasFinder	5449-5478	30	NZ_CP028284	Enterococcus faecalis strain FDAARGOS_324 plasmid unnamed2, complete sequence	4998-5027	0	1.0
NZ_SRYT01000015_1	1.2\|5449\|30\|NZ_SRYT01000015\|CRISPRCasFinder	5449-5478	30	NZ_CP030043	Enterococcus faecalis strain C25 plasmid pC25-1, complete sequence	773-802	0	1.0
NZ_SRYT01000015_1	1.1\|5395\|30\|NZ_SRYT01000015\|CRISPRCasFinder	5395-5424	30	NC_013533	Enterococcus faecalis plasmid pBEE99, complete sequence	748-777	1	0.967
NZ_SRYT01000015_1	1.2\|5449\|30\|NZ_SRYT01000015\|CRISPRCasFinder	5449-5478	30	CP002494	Enterococcus faecalis 62 plasmid EF62pC, complete sequence	7697-7726	1	0.967
NZ_SRYT01000015_1	1.2\|5449\|30\|NZ_SRYT01000015\|CRISPRCasFinder	5449-5478	30	MH830362	Enterococcus faecalis plasmid p4, complete sequence	66225-66254	1	0.967
NZ_SRYT01000015_1	1.2\|5449\|30\|NZ_SRYT01000015\|CRISPRCasFinder	5449-5478	30	NZ_CP031028	Enterococcus faecalis strain TY1 isolate flatfish plasmid pDEF-1, complete sequence	12230-12259	1	0.967
NZ_SRYT01000015_1	1.2\|5449\|30\|NZ_SRYT01000015\|CRISPRCasFinder	5449-5478	30	NZ_CP019514	Enterococcus faecalis strain CLB21560 plasmid pB, complete sequence	60960-60989	1	0.967
NZ_SRYT01000015_1	1.2\|5449\|30\|NZ_SRYT01000015\|CRISPRCasFinder	5449-5478	30	NZ_CP036248	Enterococcus faecalis R712 plasmid pR712_02, complete sequence	92808-92837	1	0.967
NZ_SRYT01000015_1	1.2\|5449\|30\|NZ_SRYT01000015\|CRISPRCasFinder	5449-5478	30	NZ_CP049777	Enterococcus faecalis strain ES-1 plasmid unnamed2, complete sequence	449-478	1	0.967
NZ_SRYT01000015_1	1.2\|5449\|30\|NZ_SRYT01000015\|CRISPRCasFinder	5449-5478	30	NZ_KY513281	Enterococcus faecalis strain 6742 plasmid p6742_2, complete sequence	5019-5048	2	0.933
NZ_SRYT01000015_1	1.2\|5449\|30\|NZ_SRYT01000015\|CRISPRCasFinder	5449-5478	30	NC_013533	Enterococcus faecalis plasmid pBEE99, complete sequence	694-723	2	0.933
NZ_SRYT01000015_1	1.2\|5449\|30\|NZ_SRYT01000015\|CRISPRCasFinder	5449-5478	30	LC499744	Enterococcus faecalis S7316 plasmid pS7316optrA DNA, complete sequence	9482-9511	2	0.933
NZ_SRYT01000015_1	1.1\|5395\|30\|NZ_SRYT01000015\|CRISPRCasFinder	5395-5424	30	NC_023573	Staphylococcus phage phiSA012 DNA, complete genome	31963-31992	6	0.8
NZ_SRYT01000015_1	1.1\|5395\|30\|NZ_SRYT01000015\|CRISPRCasFinder	5395-5424	30	MT711976	Streptomyces phage Coruscant, complete genome	87401-87430	7	0.767
NZ_SRYT01000015_1	1.2\|5449\|30\|NZ_SRYT01000015\|CRISPRCasFinder	5449-5478	30	NZ_CP022523	Pseudoalteromonas sp. NC201 plasmid pNC201, complete sequence	586542-586571	7	0.767
NZ_SRYT01000015_1	1.1\|5395\|30\|NZ_SRYT01000015\|CRISPRCasFinder	5395-5424	30	JQ660954	Clostridium phage PhiS63, complete genome	22890-22919	9	0.7

1. spacer 1.1|5395|30|NZ_SRYT01000015|CRISPRCasFinder matches to NZ_KY513281 (Enterococcus faecalis strain 6742 plasmid p6742_2, complete sequence) position: , mismatch: 0, identity: 1.0

aagtactggtattattggattcttctggac	CRISPR spacer
aagtactggtattattggattcttctggac	Protospacer
******************************

2. spacer 1.1|5395|30|NZ_SRYT01000015|CRISPRCasFinder matches to NC_004671 (Enterococcus faecalis V583 plasmid pTEF2, complete sequence) position: , mismatch: 0, identity: 1.0

aagtactggtattattggattcttctggac	CRISPR spacer
aagtactggtattattggattcttctggac	Protospacer
******************************

3. spacer 1.1|5395|30|NZ_SRYT01000015|CRISPRCasFinder matches to NZ_CP015885 (Enterococcus faecalis strain sorialis plasmid Efsorialis-p2, complete sequence) position: , mismatch: 0, identity: 1.0

aagtactggtattattggattcttctggac	CRISPR spacer
aagtactggtattattggattcttctggac	Protospacer
******************************

4. spacer 1.1|5395|30|NZ_SRYT01000015|CRISPRCasFinder matches to NZ_CP040897 (Enterococcus faecalis strain HA-1 plasmid punnamed) position: , mismatch: 0, identity: 1.0

aagtactggtattattggattcttctggac	CRISPR spacer
aagtactggtattattggattcttctggac	Protospacer
******************************

5. spacer 1.1|5395|30|NZ_SRYT01000015|CRISPRCasFinder matches to CP002494 (Enterococcus faecalis 62 plasmid EF62pC, complete sequence) position: , mismatch: 0, identity: 1.0

aagtactggtattattggattcttctggac	CRISPR spacer
aagtactggtattattggattcttctggac	Protospacer
******************************

6. spacer 1.1|5395|30|NZ_SRYT01000015|CRISPRCasFinder matches to MH830362 (Enterococcus faecalis plasmid p4, complete sequence) position: , mismatch: 0, identity: 1.0

aagtactggtattattggattcttctggac	CRISPR spacer
aagtactggtattattggattcttctggac	Protospacer
******************************

7. spacer 1.1|5395|30|NZ_SRYT01000015|CRISPRCasFinder matches to NZ_CP031028 (Enterococcus faecalis strain TY1 isolate flatfish plasmid pDEF-1, complete sequence) position: , mismatch: 0, identity: 1.0

aagtactggtattattggattcttctggac	CRISPR spacer
aagtactggtattattggattcttctggac	Protospacer
******************************

8. spacer 1.1|5395|30|NZ_SRYT01000015|CRISPRCasFinder matches to NZ_CP046109 (Enterococcus faecalis strain 133170041-3 plasmid pAD1, complete sequence) position: , mismatch: 0, identity: 1.0

aagtactggtattattggattcttctggac	CRISPR spacer
aagtactggtattattggattcttctggac	Protospacer
******************************

9. spacer 1.1|5395|30|NZ_SRYT01000015|CRISPRCasFinder matches to NZ_CP019514 (Enterococcus faecalis strain CLB21560 plasmid pB, complete sequence) position: , mismatch: 0, identity: 1.0

aagtactggtattattggattcttctggac	CRISPR spacer
aagtactggtattattggattcttctggac	Protospacer
******************************

10. spacer 1.1|5395|30|NZ_SRYT01000015|CRISPRCasFinder matches to NZ_CP036248 (Enterococcus faecalis R712 plasmid pR712_02, complete sequence) position: , mismatch: 0, identity: 1.0

aagtactggtattattggattcttctggac	CRISPR spacer
aagtactggtattattggattcttctggac	Protospacer
******************************

11. spacer 1.1|5395|30|NZ_SRYT01000015|CRISPRCasFinder matches to NZ_CP049777 (Enterococcus faecalis strain ES-1 plasmid unnamed2, complete sequence) position: , mismatch: 0, identity: 1.0

aagtactggtattattggattcttctggac	CRISPR spacer
aagtactggtattattggattcttctggac	Protospacer
******************************

12. spacer 1.1|5395|30|NZ_SRYT01000015|CRISPRCasFinder matches to NZ_CP028837 (Enterococcus faecalis strain FC plasmid unnamed2, complete sequence) position: , mismatch: 0, identity: 1.0

aagtactggtattattggattcttctggac	CRISPR spacer
aagtactggtattattggattcttctggac	Protospacer
******************************

13. spacer 1.1|5395|30|NZ_SRYT01000015|CRISPRCasFinder matches to NZ_CP030046 (Enterococcus faecalis strain C54 plasmid pC54, complete sequence) position: , mismatch: 0, identity: 1.0

aagtactggtattattggattcttctggac	CRISPR spacer
aagtactggtattattggattcttctggac	Protospacer
******************************

14. spacer 1.1|5395|30|NZ_SRYT01000015|CRISPRCasFinder matches to NC_011642 (Enterococcus faecalis plasmid pMG2200, complete sequence) position: , mismatch: 0, identity: 1.0

aagtactggtattattggattcttctggac	CRISPR spacer
aagtactggtattattggattcttctggac	Protospacer
******************************

15. spacer 1.1|5395|30|NZ_SRYT01000015|CRISPRCasFinder matches to LC499744 (Enterococcus faecalis S7316 plasmid pS7316optrA DNA, complete sequence) position: , mismatch: 0, identity: 1.0

aagtactggtattattggattcttctggac	CRISPR spacer
aagtactggtattattggattcttctggac	Protospacer
******************************

16. spacer 1.1|5395|30|NZ_SRYT01000015|CRISPRCasFinder matches to NZ_CP028284 (Enterococcus faecalis strain FDAARGOS_324 plasmid unnamed2, complete sequence) position: , mismatch: 0, identity: 1.0

aagtactggtattattggattcttctggac	CRISPR spacer
aagtactggtattattggattcttctggac	Protospacer
******************************

17. spacer 1.1|5395|30|NZ_SRYT01000015|CRISPRCasFinder matches to NZ_CP030043 (Enterococcus faecalis strain C25 plasmid pC25-1, complete sequence) position: , mismatch: 0, identity: 1.0

aagtactggtattattggattcttctggac	CRISPR spacer
aagtactggtattattggattcttctggac	Protospacer
******************************

18. spacer 1.2|5449|30|NZ_SRYT01000015|CRISPRCasFinder matches to NC_004671 (Enterococcus faecalis V583 plasmid pTEF2, complete sequence) position: , mismatch: 0, identity: 1.0

aaacgccgattttatcatgtttatccgaag	CRISPR spacer
aaacgccgattttatcatgtttatccgaag	Protospacer
******************************

19. spacer 1.2|5449|30|NZ_SRYT01000015|CRISPRCasFinder matches to NZ_CP015885 (Enterococcus faecalis strain sorialis plasmid Efsorialis-p2, complete sequence) position: , mismatch: 0, identity: 1.0

aaacgccgattttatcatgtttatccgaag	CRISPR spacer
aaacgccgattttatcatgtttatccgaag	Protospacer
******************************

20. spacer 1.2|5449|30|NZ_SRYT01000015|CRISPRCasFinder matches to NZ_CP040897 (Enterococcus faecalis strain HA-1 plasmid punnamed) position: , mismatch: 0, identity: 1.0

aaacgccgattttatcatgtttatccgaag	CRISPR spacer
aaacgccgattttatcatgtttatccgaag	Protospacer
******************************

21. spacer 1.2|5449|30|NZ_SRYT01000015|CRISPRCasFinder matches to NZ_CP046109 (Enterococcus faecalis strain 133170041-3 plasmid pAD1, complete sequence) position: , mismatch: 0, identity: 1.0

aaacgccgattttatcatgtttatccgaag	CRISPR spacer
aaacgccgattttatcatgtttatccgaag	Protospacer
******************************

22. spacer 1.2|5449|30|NZ_SRYT01000015|CRISPRCasFinder matches to NZ_CP028837 (Enterococcus faecalis strain FC plasmid unnamed2, complete sequence) position: , mismatch: 0, identity: 1.0

aaacgccgattttatcatgtttatccgaag	CRISPR spacer
aaacgccgattttatcatgtttatccgaag	Protospacer
******************************

23. spacer 1.2|5449|30|NZ_SRYT01000015|CRISPRCasFinder matches to NZ_CP030046 (Enterococcus faecalis strain C54 plasmid pC54, complete sequence) position: , mismatch: 0, identity: 1.0

aaacgccgattttatcatgtttatccgaag	CRISPR spacer
aaacgccgattttatcatgtttatccgaag	Protospacer
******************************

24. spacer 1.2|5449|30|NZ_SRYT01000015|CRISPRCasFinder matches to NC_011642 (Enterococcus faecalis plasmid pMG2200, complete sequence) position: , mismatch: 0, identity: 1.0

aaacgccgattttatcatgtttatccgaag	CRISPR spacer
aaacgccgattttatcatgtttatccgaag	Protospacer
******************************

25. spacer 1.2|5449|30|NZ_SRYT01000015|CRISPRCasFinder matches to NZ_CP028284 (Enterococcus faecalis strain FDAARGOS_324 plasmid unnamed2, complete sequence) position: , mismatch: 0, identity: 1.0

aaacgccgattttatcatgtttatccgaag	CRISPR spacer
aaacgccgattttatcatgtttatccgaag	Protospacer
******************************

26. spacer 1.2|5449|30|NZ_SRYT01000015|CRISPRCasFinder matches to NZ_CP030043 (Enterococcus faecalis strain C25 plasmid pC25-1, complete sequence) position: , mismatch: 0, identity: 1.0

aaacgccgattttatcatgtttatccgaag	CRISPR spacer
aaacgccgattttatcatgtttatccgaag	Protospacer
******************************

27. spacer 1.1|5395|30|NZ_SRYT01000015|CRISPRCasFinder matches to NC_013533 (Enterococcus faecalis plasmid pBEE99, complete sequence) position: , mismatch: 1, identity: 0.967

aagtactggtattattggattcttctggac	CRISPR spacer
aagtcctggtattattggattcttctggac	Protospacer
**** *************************

28. spacer 1.2|5449|30|NZ_SRYT01000015|CRISPRCasFinder matches to CP002494 (Enterococcus faecalis 62 plasmid EF62pC, complete sequence) position: , mismatch: 1, identity: 0.967

aaacgccgattttatcatgtttatccgaag	CRISPR spacer
aaatgccgattttatcatgtttatccgaag	Protospacer
***.**************************

29. spacer 1.2|5449|30|NZ_SRYT01000015|CRISPRCasFinder matches to MH830362 (Enterococcus faecalis plasmid p4, complete sequence) position: , mismatch: 1, identity: 0.967

aaacgccgattttatcatgtttatccgaag	CRISPR spacer
aaacgctgattttatcatgtttatccgaag	Protospacer
******.***********************

30. spacer 1.2|5449|30|NZ_SRYT01000015|CRISPRCasFinder matches to NZ_CP031028 (Enterococcus faecalis strain TY1 isolate flatfish plasmid pDEF-1, complete sequence) position: , mismatch: 1, identity: 0.967

aaacgccgattttatcatgtttatccgaag	CRISPR spacer
aaatgccgattttatcatgtttatccgaag	Protospacer
***.**************************

31. spacer 1.2|5449|30|NZ_SRYT01000015|CRISPRCasFinder matches to NZ_CP019514 (Enterococcus faecalis strain CLB21560 plasmid pB, complete sequence) position: , mismatch: 1, identity: 0.967

aaacgccgattttatcatgtttatccgaag	CRISPR spacer
aaatgccgattttatcatgtttatccgaag	Protospacer
***.**************************

32. spacer 1.2|5449|30|NZ_SRYT01000015|CRISPRCasFinder matches to NZ_CP036248 (Enterococcus faecalis R712 plasmid pR712_02, complete sequence) position: , mismatch: 1, identity: 0.967

aaacgccgattttatcatgtttatccgaag	CRISPR spacer
aaatgccgattttatcatgtttatccgaag	Protospacer
***.**************************

33. spacer 1.2|5449|30|NZ_SRYT01000015|CRISPRCasFinder matches to NZ_CP049777 (Enterococcus faecalis strain ES-1 plasmid unnamed2, complete sequence) position: , mismatch: 1, identity: 0.967

aaacgccgattttatcatgtttatccgaag	CRISPR spacer
aaacgctgattttatcatgtttatccgaag	Protospacer
******.***********************

34. spacer 1.2|5449|30|NZ_SRYT01000015|CRISPRCasFinder matches to NZ_KY513281 (Enterococcus faecalis strain 6742 plasmid p6742_2, complete sequence) position: , mismatch: 2, identity: 0.933

aaacgccgattttatcatgtttatccgaag	CRISPR spacer
aaacgttgattttatcatgtttatccgaag	Protospacer
*****..***********************

35. spacer 1.2|5449|30|NZ_SRYT01000015|CRISPRCasFinder matches to NC_013533 (Enterococcus faecalis plasmid pBEE99, complete sequence) position: , mismatch: 2, identity: 0.933

aaacgccgattttatcatgtttatccgaag	CRISPR spacer
aaacgttgattttatcatgtttatccgaag	Protospacer
*****..***********************

36. spacer 1.2|5449|30|NZ_SRYT01000015|CRISPRCasFinder matches to LC499744 (Enterococcus faecalis S7316 plasmid pS7316optrA DNA, complete sequence) position: , mismatch: 2, identity: 0.933

aaacgccgattttatcatgtttatccgaag	CRISPR spacer
aaacgttgattttatcatgtttatccgaag	Protospacer
*****..***********************

37. spacer 1.1|5395|30|NZ_SRYT01000015|CRISPRCasFinder matches to NC_023573 (Staphylococcus phage phiSA012 DNA, complete genome) position: , mismatch: 6, identity: 0.8

aagtactggtattattggattcttctggac	CRISPR spacer
aagtactggtattaaaggattcttcaaaat	Protospacer
**************  ********* ..*.

38. spacer 1.1|5395|30|NZ_SRYT01000015|CRISPRCasFinder matches to MT711976 (Streptomyces phage Coruscant, complete genome) position: , mismatch: 7, identity: 0.767

aagtactggtattattggattcttctggac	CRISPR spacer
acagagtggttttattggcttcttctggat	Protospacer
* . * **** ******* **********.

39. spacer 1.2|5449|30|NZ_SRYT01000015|CRISPRCasFinder matches to NZ_CP022523 (Pseudoalteromonas sp. NC201 plasmid pNC201, complete sequence) position: , mismatch: 7, identity: 0.767

aaacgccgattttatcatgtttatccgaag	CRISPR spacer
aaacgccgtttttatcatttttaatggtgg	Protospacer
******** ********* **** . * .*

40. spacer 1.1|5395|30|NZ_SRYT01000015|CRISPRCasFinder matches to JQ660954 (Clostridium phage PhiS63, complete genome) position: , mismatch: 9, identity: 0.7

aagtactggtattattggattcttctggac	CRISPR spacer
ttgtactgatattattggtttcttcgtttt	Protospacer
  ******.********* ******    .

Prophage detection

Region	Region Position	Protein_number	Hit_taxonomy	Key_proteins	Att_site	Prophage annotation

Anti-CRISPR protein detection

Acr_ID: NZ_SRYT01000015.1|WP_002401839.1|13423_13753_-|hypothetical-protein

Click the colored protein region to show detailed information

Acr_ID: NZ_SRYT01000015.1|WP_025188019.1|13766_14381_-|hypothetical-protein

Click the colored protein region to show detailed information

Acr ID

Acr position

Acr size

Homology with known anti

Neighbor HTH/AcRanker

Neighbor Aca

In prophage

Protospacer in prophage

NZ_SRYT01000015.1|WP_002401839.1|13423_13753_-|hypothetical-protein

13423_13753_-

109 aa aa

AcrIIA17

hit_Aca_id	identity	query_coverage	hit_coverage
anti_CRISPR3633\|WP_002401839.1\|AcrIIA17\|II-A\|Verified\|Enterococcus-faecalis	1.0	1	1.0

HTH_49

Identity	Hit_domain_coverage
NA	NA

NZ_SRYT01000015.1|WP_025188019.1|13766_14381_-|hypothetical-protein

13766_14381_-

204 aa aa

AcrIIA16

hit_Aca_id	identity	query_coverage	hit_coverage
anti_CRISPR3632\|WP_025188019.1\|AcrIIA16\|II-A\|Verified\|Enterococcus-faecalis	1.0	1	1.0

HTH_49

Identity	Hit_domain_coverage
NA	NA

3. NZ_SRYT01000003

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

147234 : 153945

Enterococcus_phage(33.33%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_002355360.1\|147234_147459_+	redoxin NrdH	A0A0M7REK7	Lactobacillus_phage	6.4e-14	49.3
WP_002355359.1\|147460_147838_+	class Ib ribonucleoside-diphosphate reductase assembly flavoprotein NrdI	D9J0S1	Brochothrix_phage	1.4e-13	34.9
WP_002355358.1\|147818_149978_+	class 1b ribonucleoside-diphosphate reductase subunit alpha	A8E2R1	Enterococcus_phage	5.9e-266	63.3
WP_002355357.1\|150178_151144_+	class 1b ribonucleoside-diphosphate reductase subunit beta	A0A096XT60	Enterococcus_phage	2.1e-130	71.3
WP_002387692.1\|151183_152470_-	TPM domain-containing protein	NA	NA	NA	NA
WP_002386098.1\|152515_153085_-	LemA family protein	A0A1X9IGG1	Lactococcus_phage	3.1e-12	28.5
WP_002355354.1\|153243_153945_-	MgtC/SapB family protein	G3MA03	Bacillus_virus	1.9e-16	40.8

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

4. NZ_SRYT01000001

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Crispr_ID: NZ_SRYT01000001_1

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

NZ_SRYT01000001_1

269829-270326

TypeII

Consensus_repeat	Method
GTTTTACTGATAAGAAATTATTGAGAGTACAAAAAC	PILER-CR
GTTTTACTGATAAGAAATTATTGAGAGTACAAAAAC	CRISPRCasFinder
GTTTTACTGATAAGAAATTATTGAGAGTACAAAAAC	CRT

7 spacers

csn2,cas2,cas9,cas3

The CRISPR arrays of NZ_SRYT01000001_1

>merge|NZ_SRYT01000001|1|269829-270326|PILER-CR,CRISPRCasFinder,CRT
GTTTTACTGATAAGAAATTATTGAGAGTACAAAAACTAAGATGGCAGAAAACCCAAAGGACCGCAAGTTTTACTGATAAGAAATTATTGAGAGTACAAAAACATGAGACAGTACCGGCTCCTGCATTACCTAGTTTTACTGATAAGAAATTATTGAGAGTACAAAAACCAGAGTTTCAAGAGCTGCATGAATTATTCGGTTTTACTGATAAGAAATTATTGAGAGTACAAAAACCTTTCAAAAGAAAATAATTGATTCAATTGAGTTTTACTGATAAGAAATTATTGAGAGTACAAAAACTCGTCTGTGCGCCGAATAGATAAAGAGGTGGTTTTACTGATAAGAAATTATTGAGAGTACAAAAACCACTATTTGGGGAGACGAGCGTGACGAGCCGTTTTACTGATAAGAAATTATTGAGAGTACAAAAACTTACACCAAAGCGAAACATATTCAACTTTTGTTTTACTGATAAGAAATTATTGAGAGTACAAAAAC

>NZ_SRYT01000001|1|1|269829-270326|PILER-CR
GTTTTACTGATAAGAAATTATTGAGAGTACAAAAAC	TAAGATGGCAGAAAACCCAAAGGACCGCAA
GTTTTACTGATAAGAAATTATTGAGAGTACAAAAAC	ATGAGACAGTACCGGCTCCTGCATTACCTA
GTTTTACTGATAAGAAATTATTGAGAGTACAAAAAC	CAGAGTTTCAAGAGCTGCATGAATTATTCG
GTTTTACTGATAAGAAATTATTGAGAGTACAAAAAC	CTTTCAAAAGAAAATAATTGATTCAATTGA
GTTTTACTGATAAGAAATTATTGAGAGTACAAAAAC	TCGTCTGTGCGCCGAATAGATAAAGAGGTG
GTTTTACTGATAAGAAATTATTGAGAGTACAAAAAC	CACTATTTGGGGAGACGAGCGTGACGAGCC
GTTTTACTGATAAGAAATTATTGAGAGTACAAAAAC	TTACACCAAAGCGAAACATATTCAACTTTT
GTTTTACTGATAAGAAATTATTGAGAGTACAAAAAC

>NZ_SRYT01000001|1|1|269829-270326|CRISPRCasFinder
GTTTTACTGATAAGAAATTATTGAGAGTACAAAAAC	TAAGATGGCAGAAAACCCAAAGGACCGCAA
GTTTTACTGATAAGAAATTATTGAGAGTACAAAAAC	ATGAGACAGTACCGGCTCCTGCATTACCTA
GTTTTACTGATAAGAAATTATTGAGAGTACAAAAAC	CAGAGTTTCAAGAGCTGCATGAATTATTCG
GTTTTACTGATAAGAAATTATTGAGAGTACAAAAAC	CTTTCAAAAGAAAATAATTGATTCAATTGA
GTTTTACTGATAAGAAATTATTGAGAGTACAAAAAC	TCGTCTGTGCGCCGAATAGATAAAGAGGTG
GTTTTACTGATAAGAAATTATTGAGAGTACAAAAAC	CACTATTTGGGGAGACGAGCGTGACGAGCC
GTTTTACTGATAAGAAATTATTGAGAGTACAAAAAC	TTACACCAAAGCGAAACATATTCAACTTTT
GTTTTACTGATAAGAAATTATTGAGAGTACAAAAAC

>NZ_SRYT01000001|1|1|269829-270326|CRT
GTTTTACTGATAAGAAATTATTGAGAGTACAAAAAC	TAAGATGGCAGAAAACCCAAAGGACCGCAA
GTTTTACTGATAAGAAATTATTGAGAGTACAAAAAC	ATGAGACAGTACCGGCTCCTGCATTACCTA
GTTTTACTGATAAGAAATTATTGAGAGTACAAAAAC	CAGAGTTTCAAGAGCTGCATGAATTATTCG
GTTTTACTGATAAGAAATTATTGAGAGTACAAAAAC	CTTTCAAAAGAAAATAATTGATTCAATTGA
GTTTTACTGATAAGAAATTATTGAGAGTACAAAAAC	TCGTCTGTGCGCCGAATAGATAAAGAGGTG
GTTTTACTGATAAGAAATTATTGAGAGTACAAAAAC	CACTATTTGGGGAGACGAGCGTGACGAGCC
GTTTTACTGATAAGAAATTATTGAGAGTACAAAAAC	TTACACCAAAGCGAAACATATTCAACTTTT
GTTTTACTGATAAGAAATTATTGAGAGTACAAAAAC

Protein	Signature genes	Signature genes Name	Protein_function
NZ_SRYT01000001.1\|WP_002357304.1\|264486_265164_-\|phosphate-signaling-complex-protein-PhoU	unknown	unknown	gnl\|CDD\|273990
NZ_SRYT01000001.1\|WP_010822901.1\|260925_261888_+\|GIY-YIG-nuclease-family-protein	unknown	unknown	gnl\|CDD\|234792
NZ_SRYT01000001.1\|WP_111997025.1\|278476_279578_-\|peptide-chain-release-factor-2	unknown	unknown	gnl\|CDD\|234799
NZ_SRYT01000001.1\|WP_087548597.1\|283813_285130_-\|DEAD/DEAH-box-helicase-family-protein	cas3	cd09639_cas3_CAS-I	gnl\|CDD\|226583
NZ_SRYT01000001.1\|WP_168354140.1\|265178_266030_-\|phosphate-ABC-transporter-ATP-binding-protein	unknown	unknown	gnl\|CDD\|172743
NZ_SRYT01000001.1\|WP_002379511.1\|283137_283824_-\|ComF-family-protein	unknown	unknown	gnl\|CDD\|223970
NZ_SRYT01000001.1\|WP_002357300.1\|266773_267658_-\|phosphate-ABC-transporter-permease-PstA	unknown	unknown	gnl\|CDD\|223654
NZ_SRYT01000001.1\|WP_002357305.1\|262677_264279_-\|daptomycin-sensing-surface-protein-LiaX	unknown	unknown	gnl\|CDD\|226123
NZ_SRYT01000001.1\|WP_002357293.1\|282447_282999_-\|ribosome-associated-translation-inhibitor-RaiA	unknown	unknown	gnl\|CDD\|224461
NZ_SRYT01000001.1\|WP_002357306.1\|262279_262603_-\|PspC-domain-containing-protein	unknown	unknown	gnl\|CDD\|377200
NZ_SRYT01000001.1\|WP_002361935.1\|277684_278371_-\|cell-division-ATP-binding-protein-FtsE	unknown	unknown	gnl\|CDD\|225438
NZ_SRYT01000001.1\|WP_002379508.1\|270403_271462_-\|hypothetical-protein	csn2	cd12217_csn2_CAS-II-A	gnl\|CDD\|213410
NZ_SRYT01000001.1\|WP_002357302.1\|265947_266757_-\|phosphate-ABC-transporter-ATP-binding-protein	unknown	unknown	gnl\|CDD\|237646
NZ_SRYT01000001.1\|WP_025193493.1\|267657_268578_-\|phosphate-ABC-transporter-permease-subunit-PstC	unknown	unknown	gnl\|CDD\|223646
NZ_SRYT01000001.1\|WP_002381826.1\|279654_282192_-\|preprotein-translocase-subunit-SecA	unknown	unknown	gnl\|CDD\|237259
NZ_SRYT01000001.1\|WP_033591492.1\|271461_271770_-\|CRISPR-associated-endonuclease-Cas2	cas2	COG3512_cas2_CAS-II-A:CAS-II-B:CAS-II-C	gnl\|CDD\|226043
NZ_SRYT01000001.1\|WP_002357298.1\|268700_269555_-\|phosphate-ABC-transporter-substrate-binding-protein-PstS	unknown	unknown	gnl\|CDD\|273991
NZ_SRYT01000001.1\|WP_087548596.1\|272907_276360_-\|type-II-CRISPR-RNA-guided-endonuclease-Cas9	cas9	cd09643_cas9_CAS-II-A:CAS-II-B:CAS-II-C	gnl\|CDD\|187774
NZ_SRYT01000001.1\|WP_002364071.1\|276807_277692_-\|ABC-transporter-permease	unknown	unknown	gnl\|CDD\|225088
NZ_SRYT01000001.1\|WP_002357307.1\|261923_262280_-\|phage-holin-family-protein	unknown	unknown	gnl\|CDD\|377198

Protein	Function_ID	Function_description	E-value
NZ_SRYT01000001.1\|WP_002357304.1\|264486_265164_-\|phosphate-signaling-complex-protein-PhoU	gnl\|CDD\|273990	TIGR02135, Uncharacterized_protein, phosphate transport system regulatory protein PhoU. This model describes PhoU, a regulatory protein of unknown mechanism for high-affinity phosphate ABC transporter systems. The protein consists of two copies of the domain described by pfam01895. Deletion of PhoU activates constitutive expression of the phosphate ABC transporter and allows phosphate transport, but causes a growth defect and so likely has some second function. [Regulatory functions, Other, Transport and binding proteins, Anions].	6.09767e-88
NZ_SRYT01000001.1\|WP_010822901.1\|260925_261888_+\|GIY-YIG-nuclease-family-protein	gnl\|CDD\|234792	PRK00558, uvrC, excinuclease ABC subunit UvrC.	1.77076e-43
NZ_SRYT01000001.1\|WP_111997025.1\|278476_279578_-\|peptide-chain-release-factor-2	gnl\|CDD\|234799	PRK00578, prfB, peptide chain release factor 2; Validated.	0
NZ_SRYT01000001.1\|WP_087548597.1\|283813_285130_-\|DEAD/DEAH-box-helicase-family-protein	gnl\|CDD\|226583	COG4098, comFA, Superfamily II DNA/RNA helicase required for DNA uptake (late competence protein) [DNA replication, recombination, and repair].	4.30063e-158
NZ_SRYT01000001.1\|WP_168354140.1\|265178_266030_-\|phosphate-ABC-transporter-ATP-binding-protein	gnl\|CDD\|172743	PRK14255, PRK14255, phosphate ABC transporter ATP-binding protein; Provisional.	0
NZ_SRYT01000001.1\|WP_002379511.1\|283137_283824_-\|ComF-family-protein	gnl\|CDD\|223970	COG1040, ComFC, Predicted amidophosphoribosyltransferases [General function prediction only].	4.80637e-34
NZ_SRYT01000001.1\|WP_002357300.1\|266773_267658_-\|phosphate-ABC-transporter-permease-PstA	gnl\|CDD\|223654	COG0581, PstA, ABC-type phosphate transport system, permease component [Inorganic ion transport and metabolism].	1.01734e-103
NZ_SRYT01000001.1\|WP_002357305.1\|262677_264279_-\|daptomycin-sensing-surface-protein-LiaX	gnl\|CDD\|226123	COG3595, COG3595, Uncharacterized conserved protein [Function unknown].	6.33034e-14
NZ_SRYT01000001.1\|WP_002357293.1\|282447_282999_-\|ribosome-associated-translation-inhibitor-RaiA	gnl\|CDD\|224461	COG1544, COG1544, Ribosome-associated protein Y (PSrp-1) [Translation, ribosomal structure and biogenesis].	9.1271e-38
NZ_SRYT01000001.1\|WP_002357306.1\|262279_262603_-\|PspC-domain-containing-protein	gnl\|CDD\|377200	pfam04024, PspC, PspC domain. This family includes Phage shock protein C (PspC) that is thought to be a transcriptional regulator. The presumed domain is 60 amino acid residues in length.	1.24173e-20
NZ_SRYT01000001.1\|WP_002361935.1\|277684_278371_-\|cell-division-ATP-binding-protein-FtsE	gnl\|CDD\|225438	COG2884, FtsE, Predicted ATPase involved in cell division [Cell division and chromosome partitioning].	1.10484e-139
NZ_SRYT01000001.1\|WP_002379508.1\|270403_271462_-\|hypothetical-protein	gnl\|CDD\|213410	cd12217, Stu0660_Csn2, Stu0660-like CRISPR/Cas system-associated protein Csn2. Csn2 is a Nmeni subtype-specific Cas protein, which may function in the adaptation process which mediates the incorporation of foreign nucleic acids into the microbial host genome. Csn 2 may interact directly with double-stranded DNA. This family of Csn2 proteins includes Stu0660, the proteins are larger than other (canonical) Csn2 proteins as they have an additional alpha-helical C-terminal domain. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and associated Cas proteins comprise a system for heritable host defense by prokaryotic cells against phage and other foreign DNA. Csn2 has been predicted to be a functional analog of Cas4 based on anti-correlated phyletic patterns; also known as SPy1049 family.	6.00722e-114
NZ_SRYT01000001.1\|WP_002357302.1\|265947_266757_-\|phosphate-ABC-transporter-ATP-binding-protein	gnl\|CDD\|237646	PRK14237, PRK14237, phosphate transporter ATP-binding protein; Provisional.	0
NZ_SRYT01000001.1\|WP_025193493.1\|267657_268578_-\|phosphate-ABC-transporter-permease-subunit-PstC	gnl\|CDD\|223646	COG0573, PstC, ABC-type phosphate transport system, permease component [Inorganic ion transport and metabolism].	2.25121e-99
NZ_SRYT01000001.1\|WP_002381826.1\|279654_282192_-\|preprotein-translocase-subunit-SecA	gnl\|CDD\|237259	PRK12904, PRK12904, preprotein translocase subunit SecA; Reviewed.	0
NZ_SRYT01000001.1\|WP_033591492.1\|271461_271770_-\|CRISPR-associated-endonuclease-Cas2	gnl\|CDD\|226043	COG3512, COG3512, CRISPR-associated protein, Cas2 homolog [Defense mechanisms].	1.31394e-24
NZ_SRYT01000001.1\|WP_002357298.1\|268700_269555_-\|phosphate-ABC-transporter-substrate-binding-protein-PstS	gnl\|CDD\|273991	TIGR02136, ptsS_2, phosphate binding protein. Members of this family are phosphate-binding proteins. Most are found in phosphate ABC-transporter operons, but some are found in phosphate regulatory operons. This model separates members of the current family from the phosphate ABC transporter phosphate binding protein described by TIGRFAMs model TIGR00975. [Transport and binding proteins, Anions].	3.17732e-122
NZ_SRYT01000001.1\|WP_087548596.1\|272907_276360_-\|type-II-CRISPR-RNA-guided-endonuclease-Cas9	gnl\|CDD\|187774	cd09643, Csn1, CRISPR/Cas system-associated protein Cas9. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and associated Cas proteins comprise a system for heritable host defense by prokaryotic cells against phage and other foreign DNA; Very large protein containing McrA/HNH-nuclease related domain and a RuvC-like nuclease domain; signature gene for type II.	1.50785e-115
NZ_SRYT01000001.1\|WP_002364071.1\|276807_277692_-\|ABC-transporter-permease	gnl\|CDD\|225088	COG2177, FtsX, Cell division protein [Cell division and chromosome partitioning].	4.28311e-97
NZ_SRYT01000001.1\|WP_002357307.1\|261923_262280_-\|phage-holin-family-protein	gnl\|CDD\|377198	pfam04020, Phage_holin_4_2, Mycobacterial 4 TMS phage holin, superfamily IV. These proteins are predicted transmembrane proteins with probably four transmembrane spans. The 1.E.40 is represented by the mycobacterial 4 phage holin, but it also contains many cyanobacterial. proteobacterial and firmicute proteins. Holins are encoded within the genomes of Gram-positive and Gram-negative bacteria as well as in those of the bacteriophage of these organisms. The primary function of holins appears to be transport of murein hydrolases across the cytoplasmic membrane to the cell wall where these enzymes hydrolyze the cell wall polymer as a prelude to cell lysis. When chromosomally encoded the enzymes are therefore autolysins. Holins may also facilitate leakage of electrolytes and nutrients from the cell cytoplasm, thereby promoting cell death. Some may catalyze export of nucleases.	7.53845e-22

>NZ_SRYT01000001.1|WP_002357298.1|268700_269555_-|phosphate-ABC-transporter-substrate-binding-protein-PstS
MKKRLLLFIGLASILTLTGCAKWIDRGESITAVGSSALQPLVETASEEYQSQNPGRFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKKGIKAEDLIDHKVAVVGITPIVNKNVGVKDISMENLKKIFLGEVTNWKELGGKDQKIVILNRAAGSGTRATFEKWVLGDKTAIRAQEQDSSGMVRSIVSDTPGAISYTAFSYVTDEVATLSIDGVQPTDENVMNNKWIIWSYEHMYTRKNPSDLTKEFLDFMLSDDIQERVIGQLGYIPVSKMEIERDWQGNVIK
>NZ_SRYT01000001.1|WP_025193493.1|267657_268578_-|phosphate-ABC-transporter-permease-subunit-PstC
MEDVQKKLLTKSKRAKMEQRGKFISFLCIALIVAVVVSIFYFVASKGLATFFKDKINVLDFLFGTNWNPSDIGADGKPMVGALPMIAGSFIVTFLSAIVATPFAIGAAVFMTEISPKKGAKFLQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFGILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGILTAVVFGMARAFGEALAIQMVIGNAAVMPTSLTTPASTLTSILTMGIGNTIMGTVENNVLWSLALILLLMSLLFNIVIRIIGKRGALK
>NZ_SRYT01000001.1|WP_002357300.1|266773_267658_-|phosphate-ABC-transporter-permease-PstA
MNAKKADKIATGILYAVSGVIVLILAALLIYILARGIPHISWEFLTQPSRAYQVGGGIGIQLFNSLYLLLITMIISIPISLGAGIYLSEYAKKNWLTDLIRTSIEILSSLPSVVVGLFGFLIFVVQIGYGFSILSGALALTFFNLPLLTRNVEESLKAIHYTQREAGLALGLSRWETVKKVILPEALPGILTGVILSSGRIFGEAAALIYTAGQSAPALDFSNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGAAVSAGASAVLILVVLLFNFGARFIGNRLYKRMTSA
>NZ_SRYT01000001.1|WP_002357302.1|265947_266757_-|phosphate-ABC-transporter-ATP-binding-protein
MKEYNLNDTHLLQLDSQKDPIALHTEDLHVFYGDNEAIKGVDLQFEKNKITALIGPSGCGKSTYLRSLNRMNDGIANSRVTGKIMYKDVDVNTKEVDVYEMRKRIGMVFQRPNPFSKSIYENITFALKQHGEKDKKKLDEIVETSLKQAALWDQVKDNLNKSALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKDDYTIIIVTHNMQQAARISDYTAFFYMGKVIEYDHTRKIFTRPKIQATEDYVSGHFG
>NZ_SRYT01000001.1|WP_168354140.1|265178_266030_-|phosphate-ABC-transporter-ATP-binding-protein
MNTIIQEKSLHDQKFKQRKTTFQDILVRRSKMGKEIISSKDLHLYYGKKEALKGIDLTFNQGELTAMIGPSGCGKSTYLRCLNRMNDLIPDVTITGSVVYKGKDIYGPKTDNVELRKEIGMVFQQPNPFPFSVYENVIYGLRLKGVKDKQVLDEAVETSLKAAAVWEDVKDKLHKSALSLSGGQQQRVCIARVLAVEPDIILLDEPTSALDPVSSGKIENMLLTLKEKYTMIMVTHNMSQASRISDKTAFFLQGDLIEFNDTKKVFLNPKEKQTEDYISGKFG
>NZ_SRYT01000001.1|WP_002357304.1|264486_265164_-|phosphate-signaling-complex-protein-PhoU
MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVKAYINHDKKLAQEVIDRDIEINDMEVKLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSIAKSTIRLKGETRIPEIEKEISDMSDYVKKMVDNVLIAYVKTDQKDARMIAQMDDRTNEYFDSIYKHAVEAMKANPETVISGTDYLHVAQYLERIGDYVTNICEWIVYLATGKITELNSNRDEQF
>NZ_SRYT01000001.1|WP_002357305.1|262677_264279_-|daptomycin-sensing-surface-protein-LiaX
MKERERVLELVKKGILTSEEALILLENMATEKDEKQIEKAAEKVDTQNIGTTNKEDQVADLMDALEKGESEGPTVDSFEENKQDSAEKDRENLERILDELATKANRASAELDEVNAEIAGIKEEIKEVAEEIGTLDTKEELDALTEDEQVQRKDLHVLLAQLEEKLATQSTEKTALEEELKNIRKEQWKGQWNDTKEKVSSQFSEEWKDQATDTFNQVGGKVAEVGGQVGEFLKKTFNSFSDTMNDNVEWKDIKMKVPGVATTKFEHEFNYPNPQASLIDVKVANGTVVFKTWDQEDVKVEAKIKLYGKMAGDSPMEAFLERSDIDVDDETISFQVPNKRVKADLTFYLPKRTYDHVSVKLLNGNVLVEELTAKDVYTKSTNGTITFKKIDATMLEIEGVNGEIKVLEGTILDNIIETVNGDVSISAAPESLSVSLINGDIRITAKEKTLRRVEASSANGNIKLALPNDLGVEGQVKTNLGSINSRLTDIEVVREKKDRGNQQLHFRRVLEESMAQINASTTTGSIFLKDTDK
>NZ_SRYT01000001.1|WP_002357306.1|262279_262603_-|PspC-domain-containing-protein
MKRKLTKSPNNVVLTGSLAGIADWLGIDPTIIRVVYVLLSFFSAGFPGILLYIALAVLIPSGRTGSDRGYGHQNPYNRNVRNENPYAANKKQRKEAEKIDDDEWSDF
>NZ_SRYT01000001.1|WP_002357307.1|261923_262280_-|phage-holin-family-protein
MTYFQRLVVNTLTFISLSVVFPGMIHVRGIMPAIIAAFVLSILNMLVKPVLTILSLPFTLLTFGLFSFVVNAAILQMTSFFVGEMNFGFSSFWAAVLMAVIMSIVNTIVTDHNLSKYE
>NZ_SRYT01000001.1|WP_010822901.1|260925_261888_+|GIY-YIG-nuclease-family-protein
MKEYLKEKAHQLPLTPGVYLMKDATDTIIYVGKAKKLKNRVSSYFINSNQHSRKTMRLIKQIIDFDVLHTDTELDALLLECQLIQQYRPRYNRQMNAYEQYSYVSVAVNERQLEIKLLNIPTKENCFGPYSIRRKLNRLKIILDSIYDLVPTNYWHQTFQKESAIPTELFQQELFDFFHNQGREPIKRIAQQMQEVAEKQAFEKAAKLKEDWLFLTRFANQNQRISQANQRDWQLMWLPCQKKIKYYLIYQGLVVATRVVTKQTFQKYSPLELAKKIMPKKPPKTIEHYSKQQVDFLNILYGYISRHPECHLENLTIEKA
>NZ_SRYT01000001.1|WP_002379508.1|270403_271462_-|hypothetical-protein
MKQIRLEYQYQQFIEFSLEDYVFFYGGENQWRRKILRTLKRFAKQKALSDLEESVYGDNGIEIFLEEKKLKAKSIDFYFLEDNTSIFQQMNFIKGTLLHQELLYLQDDFDITNQMSNLNDQLLKLENLMNDKLNNYLTNIEPHFLEMNFQDILKYSLSLNYLENGQSLPLEMMDTRELLAEFIYLLQRSISRREQPVWLVITNPESFLETESIYYLFQQLKKLAQETKQLKFFIFSNRSLPLPYTEEDVEKTILLYDKYQQLPVFDVFRQSIERHYPDQLSWTNQQLIDAFYRVCHFVGDQYTKNYLLPKDMILLKLLKELLDDNSECVETSMEKLTVLEEQYFRERLLRKE
>NZ_SRYT01000001.1|WP_033591492.1|271461_271770_-|CRISPR-associated-endonuclease-Cas2
MRLMCLFDLPFETKQEQKEYRIFRKHLLENGFIMLQYSIYYRSLPNRSALKKYETILKRKVPRSGNVRLLYVSEIQFQDMLLLAGSRSHQEEVVGVHRLVVI
>NZ_SRYT01000001.1|WP_087548596.1|272907_276360_-|type-II-CRISPR-RNA-guided-endonuclease-Cas9
MYSIGLDLGISSVGWSVIDERTGNVIDLGVRLFSAKNSEKNLERRTNRGGRRLIRRKTNRLKDAKKILAAVGFYEDKSLKNSCPYQLRVKGLTEPLSKGEIYKVTLHILKKRGISYLDEDDTEAAKESQDYKEQVRENAQLLTKYTPGQIQLQRLKENNRVKTGINAQGNYQLNVFKVSAYANELATILKTQQAFYPNELTDDWIALFVQPGIAEEAGLIYRKRPYYHGPGNEANNSPYGRWSDFKKTGQPATNIFDKLIGKDFQGELRASGLSLSAQQYNLLNDLTNLKIDGEIPLSPEQKEYILAELMTKEFTRFGVNDVVKLLGVKKERLSGWRLDKKGKPEIHTLKGYRNWRKIFAESGIDLAMLPTETIDCLAKVLTLNTEREGIENTLAFELPELAESVKLLVLDRYKELSQSVSTQAWHRFSLKTLHLLIPELMNATSEQNTLLEQFQLKSDVRKRYSEYKKLPTKDVLTEIYNPTVNKTVSQAFKVIDALLVKYGKEQIRYITIEMPRDDNEEDEKKRIKELHAKNSQRKNDSQSYFMQKSGWSQEKFQTTIQKNRRFLAKLLYYYEQDGICAYTGLSISSELLVSDSTEIDHIIPISISLDDSINNKVLVLSKANQVKGQQTPYDAWMDGSFKKINGKFSNWDDYQKWVESRHFSHKKENNLLETRNIFDSEQVEKFLARNLNDTRYASRLVLNTLQSFFANQETKVRVVNGSFTHTLRKKWGADLDKTRETHHHHAVDATLCAVTPFVKVSRYHYAVKEETGEKVMREIDFETGEIVDEMSYREFKKSKKYERKTYQVKWPNFREQLKPVNLHPRIKFSHQVDRKANRKLSDATIYSVREKTEVKTLKSGKQKITTDEYTIGKIKDIYTVDGWEAFKKKQDKLLMKDLDEKTYERLLSIAETTPDFQEVEEKNGKVKRVKRSPFAVYCEENDIPAIRKYAKKNNGPLIRSLKYYDGKLNKHINITKDSQGRPVEKTKNGRKVTLQSLKPYRYDIYQDLETKAYYTVQLYYSDLRFVEGKYGITEKEYMKKVAEQTKGQVVRFCFSLQKNDGLEIEWKDSQRYDVRFYNFQSANSINFKGLEQEMMPAENQFKQKPYNNGAINLNIAKYGKEGKKLRKFNTDILGKKHYLYYEKEPKNIIK
>NZ_SRYT01000001.1|WP_002364071.1|276807_277692_-|ABC-transporter-permease
MIRTFFSHLFESIKSLKRNGWMTVASASAVTITLVLVGIFMGVIFNATKLADDIEKNVTVSVFVDIGTKQNEMKTLEKQLKGLDNVEDISYSNKDQQLKKIQEQMGDAWNLFEGDSNPLYDVYYVRAKTPEDTKDIAKQAAKFPSVFKADYGGVNSDKIFKIAQTVRTWGLAAAALLLFVAVFLISNTIRITILSRQKEIQIMRLVGAKNSFIRWPFFLEGAWIGLIGAIVPVIIMTLGYHQVYNMFNPQLLRSNYSLIRPEDFIWKVNLLMIATGMIIGSLGSVISMRRFLKI
>NZ_SRYT01000001.1|WP_002361935.1|277684_278371_-|cell-division-ATP-binding-protein-FtsE
MIEMKDVMKKYSNGTTAIRNISVEIDQGEFVYVVGPSGAGKSTFIRLMYREEKATKGNLTVAGHDLMKIKNKEVPYLRREIGIVFQDYKLLPKKTVYENVAYAMQVIGRRPREIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLIADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRDQVEGEYGYDD
>NZ_SRYT01000001.1|WP_111997025.1|278476_279578_-|peptide-chain-release-factor-2
MENPEIRTLLDEMTQKITSFRRSLDLDQLEEDIAEAENRMGEPGFWDNTETAQKLINETNSLKEKYQQFHQLSEELDELTVMFELQQEEYDAEIQEELEERIHLLQERLTTYELSLLLNEPYDHNNALIELHPGAGGTESQDWGSMLLRMYTRWAESHGFQVETLDYQAGDEAGIKSVTLLIKGYNAYGYLKSEKGVHRLVRISPFDSAKRRHTSFCSVDVMPELDDTIEIAINPDDLKIDTYRASGAGGQHINKTESAVRITHIPTGTVVASQAQRSQLKNREQAMSMLKAKLYQLEMEKKAQEAASLRGEQMEIGWGSQIRSYVFHPYSMVKDHRTNYETGNVQAVMDGDLDGFIDAYLKQRLS
>NZ_SRYT01000001.1|WP_002381826.1|279654_282192_-|preprotein-translocase-subunit-SecA
MANFLKKMIENDKKELRRLEKIADKIDAHASAMEQLSDEQLREKTDEFKARYQKGETLDELLPEAFAVVREAAKRVLGLFPYRVQLMGGIVLHDGNIPEMRTGEGKTLTATMPVYLNALSGEGVHVVTVNEYLATRDSNEMGELYNFLGLSVGLNINSKSSDEKREAYNCDITYSTNNELGFDYLRDNMVVYRSQMVQRPLNYAIVDEVDSILIDEARTPLIISGQAEKSTALYTRADNFVKRLKEDEDYKIDIQSKTIGLTEAGIEKAEQTFGLDNLYDIENTALTHHLDQALRANYIMLLDIDYVVQDNKVLIVDQFTGRIMDGRRYSDGLHQAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVIQIPTNRPIIRDDRPDLLYPTLESKFNAVVEDIKERYHKGQPVLVGTVAVETSELLSDKLNAAKIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVLELGGLAVIGTERHESRRIDNQLRGRAGRQGDPGVSQFYLSLEDDLMKRFGSERIKTFLERMNVQEEDAVIQSKMFTRQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYAQRQEVIMEENDLSDVLMGMVKRTIGRVVDSHTQLEKEEWNLDGIVDFAASTLVHEDTISKKDLENKSAEEIKDYLVARAQEVFEEKSQQLNGQEQLLEFEKVVILRVVDTKWTDHIDAMDQLRQSVGLRAYGQNNPLVEYQTEGYSMYNNMVGSIEYEVTRLFMKSEIRQNVQREQVAQGQAEHPETEQDAAAQSNTSAKRQPVRVDKKVGRNDLCPCGSGKKFKNCHGRNA
>NZ_SRYT01000001.1|WP_002357293.1|282447_282999_-|ribosome-associated-translation-inhibitor-RaiA
MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDSPEATVHVNLKVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRETGRNNTKAAVFLVGEETEETPSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFEDAETNGTSIVYRRKDGKYGLIETD
>NZ_SRYT01000001.1|WP_002379511.1|283137_283824_-|ComF-family-protein
MDCKNCASPIRLEITLKMLLRFKKWARPSLCQHCQVKFQKLPMTGTCFGCSRVSQEHYCFDCQRWQLLYPEYSFHNTALFHYDEGMQEWMERYKFQGDYRLRTCFNEEINFYFRQQSVDYIIPIPLSEKRMQKRGFNQVIGLLEAADVPYSPFLIRKEENVPQSKKTRKERMRLQQPFAIQKENQKKLKNCSVILVDDIYTTGRTLFHAAAVINDCYPKSLNTFTLAR
>NZ_SRYT01000001.1|WP_087548597.1|283813_285130_-|DEAD/DEAH-box-helicase-family-protein
MEDLHGRKIILEETEPSIQTKLVYLPTMLERSPLTIQCQRCGEVVSKKENRLATNVYYCHACIQLGRVTSCQKFCHLPERPNSPRTVFFEWSGQLTKGQQAISVELCETAKARENRLVWAVTGAGKTEMLFAVLHQTLQEGGRIALASPRVDVCLELFPRIQAVFPHEAIALLHGNSQEPYRYTKLVICTTHQLLKFHQAFDLLIVDEVDSFPFVNNEHLYYGVRNARKKRSSLVYLTATPTKELRKLLQQKKLAASILPARYHRRPLPVPRRFWVQSWQRISSKNIKIIAHHLKHLLVRNSVLLFCPSISLIHQLHPLIAERFSDKEVVAVYGSDELRLEKVQAMRQQKTDILITTTILERGVTFDAVSVIVYGANHRVFTSSTLVQIAGRVDRRQEFNYGEVLFLHDGETRDMKEAIRQIKQMNRLASKRGMLDGL

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Crispr_ID: NZ_SRYT01000001_2

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

NZ_SRYT01000001_2

527698-527982

Orphan

II-A,II-B:NA

Consensus_repeat	Method
AAACAACATGACTCTAAAAC	CRT
TTGTTTTGGTACCATTCCAAACAACATGACTCTAAAACT	CRISPRCasFinder

4 spacers

The CRISPR arrays of NZ_SRYT01000001_2

>merge|NZ_SRYT01000001|2|527698-527982|CRT,CRISPRCasFinder
TAACAACATGGCTATAAAACAGGGGAGAAAAAGCCAAATCAAAAAAGTTTGTTTTGGTACCATTCCAAACAACATGACTCTAAAACTATAGTCATTATGCTGAACAAGGGTTTGTTGTTTTGGTACCATTCTAAACAACATGACTCTAAAACTCATACGTGTTTGGTACTTCTTACCATGTTGTTTTGGCACCATTCTAAACAACATGACTCTAAAACAGGTAAGAAAAGATACCTATGCGGAAGTTTGTTTTGGTACCATTCCAAACAACATGACTCTAAAACC

>NZ_SRYT01000001|2|2|527698-527981|CRT
TAACAACATGGCTATAAAAC	AGGGGAGAAAAAGCCAAATCAAAAAAGTTTGTTTTGGTACCATTCC
AAACAACATGACTCTAAAAC	TATAGTCATTATGCTGAACAAGGGTTTGTTGTTTTGGTACCATTCT
AAACAACATGACTCTAAAAC	TCATACGTGTTTGGTACTTCTTACCATGTTGTTTTGGCACCATTCT
AAACAACATGACTCTAAAAC	AGGTAAGAAAAGATACCTATGCGGAAGTTTGTTTTGGTACCATTCC
AAACAACATGACTCTAAAAC

>NZ_SRYT01000001|2|2|527746-527982|CRISPRCasFinder
TTGTTTTGGTACCATTCCAAACAACATGACTCTAAAACT	ATAGTCATTATGCTGAACAAGGGTTTG
TTGTTTTGGTACCATTCTAAACAACATGACTCTAAAACT	CATACGTGTTTGGTACTTCTTACCATG
TTGTTTTGGCACCATTCTAAACAACATGACTCTAAAACA	GGTAAGAAAAGATACCTATGCGGAAGT
TTGTTTTGGTACCATTCCAAACAACATGACTCTAAAACC

Protein	Signature genes	Signature genes Name	Protein_function
NZ_SRYT01000001.1\|WP_002356983.1\|514450_515152_-\|GntR-family-transcriptional-regulator	unknown	unknown	gnl\|CDD\|225099
NZ_SRYT01000001.1\|WP_002379587.1\|537477_538452_-\|ribose-phosphate-diphosphokinase	unknown	unknown	gnl\|CDD\|235039
NZ_SRYT01000001.1\|WP_002379583.1\|528138_529002_-\|AraC-family-transcriptional-regulator	unknown	unknown	gnl\|CDD\|214874
NZ_SRYT01000001.1\|WP_002381711.1\|513583_514333_-\|ABC-transporter-ATP-binding-protein	unknown	unknown	gnl\|CDD\|224059
NZ_SRYT01000001.1\|WP_002378477.1\|523108_524842_-\|thiol-reductant-ABC-exporter-subunit-CydD	unknown	unknown	gnl\|CDD\|274323
NZ_SRYT01000001.1\|WP_002379584.1\|531761_532331_-\|TetR/AcrR-family-transcriptional-regulator	unknown	unknown	gnl\|CDD\|379115
NZ_SRYT01000001.1\|WP_002360015.1\|520285_521266_+\|polyprenyl-synthetase-family-protein	unknown	unknown	gnl\|CDD\|223220
NZ_SRYT01000001.1\|WP_002362074.1\|532995_534234_+\|multidrug-efflux-MFS-transporter	unknown	unknown	gnl\|CDD\|340949
NZ_SRYT01000001.1\|WP_087548645.1\|536175_537321_-\|cysteine-desulfurase	unknown	unknown	gnl\|CDD\|224029
NZ_SRYT01000001.1\|WP_002379580.1\|518018_519230_-\|NAD(P)/FAD-dependent-oxidoreductase	unknown	unknown	gnl\|CDD\|224172
NZ_SRYT01000001.1\|WP_002362072.1\|529086_531168_-\|DNA-topoisomerase-III	unknown	unknown	gnl\|CDD\|236078
NZ_SRYT01000001.1\|WP_002360016.1\|519258_520164_-\|prenyltransferase	unknown	unknown	gnl\|CDD\|235695
NZ_SRYT01000001.1\|WP_002356974.1\|525856_527275_-\|cytochrome-ubiquinol-oxidase-subunit-I	unknown	unknown	gnl\|CDD\|376587
NZ_SRYT01000001.1\|WP_002356966.1\|531298_531664_-\|DUF1033-family-protein	unknown	unknown	gnl\|CDD\|336361
NZ_SRYT01000001.1\|WP_002379582.1\|524846_525860_-\|cytochrome-d-ubiquinol-oxidase-subunit-II	unknown	unknown	gnl\|CDD\|376764
NZ_SRYT01000001.1\|WP_002379586.1\|532463_532979_+\|YbhB/YbcL-family-Raf-kinase-inhibitor-like-protein	unknown	unknown	gnl\|CDD\|176643
NZ_SRYT01000001.1\|WP_002379581.1\|521345_523112_-\|thiol-reductant-ABC-exporter-subunit-CydC	unknown	unknown	gnl\|CDD\|227320
NZ_SRYT01000001.1\|WP_002379579.1\|515281_517705_-\|DNA-translocase-FtsK	unknown	unknown	gnl\|CDD\|224588
NZ_SRYT01000001.1\|WP_002356959.1\|535763_536108_-\|DUF1831-domain-containing-protein	unknown	unknown	gnl\|CDD\|370163
NZ_SRYT01000001.1\|WP_002362075.1\|534413_535538_-\|tRNA-2-thiouridine(34)-synthase-MnmA	unknown	unknown	gnl\|CDD\|234664

Protein	Function_ID	Function_description	E-value
NZ_SRYT01000001.1\|WP_002356983.1\|514450_515152_-\|GntR-family-transcriptional-regulator	gnl\|CDD\|225099	COG2188, PhnF, Transcriptional regulators [Transcription].	5.36718e-38
NZ_SRYT01000001.1\|WP_002379587.1\|537477_538452_-\|ribose-phosphate-diphosphokinase	gnl\|CDD\|235039	PRK02458, PRK02458, ribose-phosphate pyrophosphokinase; Provisional.	0
NZ_SRYT01000001.1\|WP_002379583.1\|528138_529002_-\|AraC-family-transcriptional-regulator	gnl\|CDD\|214874	smart00871, AraC_E_bind, Bacterial transcription activator, effector binding domain. This domain is found in the probable effector binding domain of a number of different bacterial transcription activators.and is also present in some DNA gyrase inhibitors. The absence of a HTH motif in the DNA gyrase inhibitors is thought to indicate the fact that these do not bind DNA.	2.64372e-26
NZ_SRYT01000001.1\|WP_002381711.1\|513583_514333_-\|ABC-transporter-ATP-binding-protein	gnl\|CDD\|224059	COG1136, SalX, ABC-type antimicrobial peptide transport system, ATPase component [Defense mechanisms].	2.63593e-110
NZ_SRYT01000001.1\|WP_002378477.1\|523108_524842_-\|thiol-reductant-ABC-exporter-subunit-CydD	gnl\|CDD\|274323	TIGR02857, transport_ATP-binding_protein, thiol reductant ABC exporter, CydD subunit. The gene pair cydCD encodes an ABC-family transporter in which each gene contains an N-terminal membrane-spanning domain (pfam00664) and a C-terminal ATP-binding domain (pfam00005). In E. coli these genes were discovered as mutants which caused the terminal heme-copper oxidase complex cytochrome bd to fail to assemble. Recent work has shown that the transporter is involved in export of redox-active thiol compounds such as cysteine and glutathione. The linkage to assembly of the cytochrome bd complex is further supported by the conserved operon structure found outside the gammaproteobacteria (cydABCD) containing both the transporter and oxidase genes components. The genes used as the seed members for this model are all either found in the gammproteobacterial context or the CydABCD context. All members of this family scoring above trusted at the time of its creation were from genomes which encode a cytochrome bd complex. Unfortunately, the gene symbol nomenclature adopted based on this operon in B. subtilis assigns cydC to the third gene in the operon where this gene is actually homologous to the E. coli cydD gene. We have chosen to name all homologs in this family in accordance with the precedence of publication of the E. coli name, CydD.	0
NZ_SRYT01000001.1\|WP_002379584.1\|531761_532331_-\|TetR/AcrR-family-transcriptional-regulator	gnl\|CDD\|379115	pfam13305, WHG, WHG domain. This presumed domain is around 80 amino acids in length. It is found to the C-terminus of a DNA-binding helix-turn-helix domain. This domain may be involved in binding to an as yet unknown ligand that allows a transcriptional regulation response to that molecule. The domain is named WHG after three conserved residues near the C-terminus of the domain.	1.88451e-15
NZ_SRYT01000001.1\|WP_002360015.1\|520285_521266_+\|polyprenyl-synthetase-family-protein	gnl\|CDD\|223220	COG0142, IspA, Geranylgeranyl pyrophosphate synthase [Coenzyme metabolism].	2.52576e-80
NZ_SRYT01000001.1\|WP_002362074.1\|532995_534234_+\|multidrug-efflux-MFS-transporter	gnl\|CDD\|340949	cd17391, MFS_MdtG_MDR_like, Multidrug resistance protein MdtG and similar multidrug resistance (MDR) transporters of the Major Facilitator Superfamily. This subfamily is composed of Escherichia coli multidrug resistance protein MdtG, Streptococcus pneumoniae multidrug resistance efflux pump PmrA, and similar multidrug resistance (MDR) transporters from bacteria. MDR transporters are drug/H+ antiporters (DHA) that mediate the efflux of a variety of drugs and toxic compounds, and confer resistance to these compounds. MdtG confers resistance to fosfomycin and deoxycholate. PmrA serves as an efflux pump for various substrates and is associated with fluoroquinolone resistance. MdtG-like MDR transporters belong to the bacterial MdtG-like and eukaryotic solute carrier 18 (SLC18) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.	9.51661e-174
NZ_SRYT01000001.1\|WP_087548645.1\|536175_537321_-\|cysteine-desulfurase	gnl\|CDD\|224029	COG1104, NifS, Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes [Amino acid transport and metabolism].	3.36146e-178
NZ_SRYT01000001.1\|WP_002379580.1\|518018_519230_-\|NAD(P)/FAD-dependent-oxidoreductase	gnl\|CDD\|224172	COG1252, Ndh, NADH dehydrogenase, FAD-containing subunit [Energy production and conversion].	4.15123e-121
NZ_SRYT01000001.1\|WP_002362072.1\|529086_531168_-\|DNA-topoisomerase-III	gnl\|CDD\|236078	PRK07726, PRK07726, DNA topoisomerase 3.	0
NZ_SRYT01000001.1\|WP_002360016.1\|519258_520164_-\|prenyltransferase	gnl\|CDD\|235695	PRK06080, PRK06080, 1,4-dihydroxy-2-naphthoate octaprenyltransferase; Validated.	8.82053e-105
NZ_SRYT01000001.1\|WP_002356974.1\|525856_527275_-\|cytochrome-ubiquinol-oxidase-subunit-I	gnl\|CDD\|376587	pfam01654, Cyt_bd_oxida_I, Cytochrome bd terminal oxidase subunit I. This family are the alternative oxidases found in many bacteria which oxidize ubiquinol and reduce oxygen as part of the electron transport chain. This family is the subunit I of the oxidase E. coli has two copies of the oxidase, bo and bd', both of which are represented here In some nitrogen fixing bacteria, e.g. Klebsiella pneumoniae this oxidase is responsible for removing oxygen in microaerobic conditions, making the oxidase required for nitrogen fixation. This subunit binds a single b-haem, through ligands at His186 and Met393 (using SW:P11026 numbering). In addition His19 is a ligand for the haem b found in subunit II.	0
NZ_SRYT01000001.1\|WP_002356966.1\|531298_531664_-\|DUF1033-family-protein	gnl\|CDD\|336361	pfam06279, DUF1033, Protein of unknown function (DUF1033). This family consists of several hypothetical bacterial proteins. Many of the sequences in this family are annotated as putative DNA binding proteins but the function of this family is unknown.	4.62165e-64
NZ_SRYT01000001.1\|WP_002379582.1\|524846_525860_-\|cytochrome-d-ubiquinol-oxidase-subunit-II	gnl\|CDD\|376764	pfam02322, Cyt_bd_oxida_II, Cytochrome bd terminal oxidase subunit II. This family consists of cytochrome bd type terminal oxidases that catalyze quinol-dependent, Na+-independent oxygen uptake. Members of this family are integral membrane proteins and contain a protohaem IX centre B558. One member of the family, Klebsiella pneumoniae CydB, is implicated in having an important role in micro-aerobic nitrogen fixation in the enteric bacterium Klebsiella pneumoniae. The family forms an integral functional unit with subunit I, family Bac_Ubq_Cox, pfam01654.	1.4291e-102
NZ_SRYT01000001.1\|WP_002379586.1\|532463_532979_+\|YbhB/YbcL-family-Raf-kinase-inhibitor-like-protein	gnl\|CDD\|176643	cd00865, PEBP_bact_arch, PhosphatidylEthanolamine-Binding Protein (PEBP) domain present in bacteria and archaea. PhosphatidylEthanolamine-Binding Proteins (PEBPs) are represented in all three major phylogenetic divisions (eukaryotes, bacteria, archaea). The members in this subgroup are present in bacterial and archaea. Members here include Escherichia coli YBHB and YBCL which are thought to regulate protein phosphorylation as well as Sulfolobus solfataricus SsCEI which inhibits serine proteases alpha-chymotrypsin and elastase. Although their overall structures are similar, the members of the PEBP family have very different substrates and oligomerization states (monomer/dimer/tetramer). In a few of the bacterial members present here the dimerization interface is proposed to form the ligand binding site, unlike in other PEBP members.	2.51831e-30
NZ_SRYT01000001.1\|WP_002379581.1\|521345_523112_-\|thiol-reductant-ABC-exporter-subunit-CydC	gnl\|CDD\|227320	COG4987, CydC, ABC-type transport system involved in cytochrome bd biosynthesis, fused ATPase and permease components [Energy production and conversion / Posttranslational modification, protein turnover, chaperones].	0
NZ_SRYT01000001.1\|WP_002379579.1\|515281_517705_-\|DNA-translocase-FtsK	gnl\|CDD\|224588	COG1674, FtsK, DNA segregation ATPase FtsK/SpoIIIE and related proteins [Cell division and chromosome partitioning].	4.95347e-175
NZ_SRYT01000001.1\|WP_002356959.1\|535763_536108_-\|DUF1831-domain-containing-protein	gnl\|CDD\|370163	pfam08866, DUF1831, Putative amino acid metabolism. Solution of the structure of the Lactobacillus plantarum protein from this family has indicated a potential new fold with remote similarities to TBP-like (TATA-binding protein) structures. This similarity, in combination with genomic context analysis, leads us to propose an involvement in amino-acid metabolism. The potentially novel fold is an alpha + beta fold comprising two beta sheets packed against a single helix. The enzyme is present in the cytosol.	1.33245e-53
NZ_SRYT01000001.1\|WP_002362075.1\|534413_535538_-\|tRNA-2-thiouridine(34)-synthase-MnmA	gnl\|CDD\|234664	PRK00143, mnmA, tRNA-specific 2-thiouridylase MnmA; Reviewed.	0

>NZ_SRYT01000001.1|WP_002356974.1|525856_527275_-|cytochrome-ubiquinol-oxidase-subunit-I
MFDIVTLARIQFAMTTVFHFFFVPFSIGLALVVAIMETMYVVKKEERYRKMAKFWGNIFLLSFAVGVVTGIIQEFQFGMNWSDYSRFVGDIFGAPLAVEALLAFFLESTFLGLWIFTWDKMNPKVHVTFIWLVVFGSMMSAFWILVANSFMQHPVGYVINNGRAEMVDFVAVISNPKVWYEFSHVIMGAFTLGGMAVAGMAAFQLLKKRDISFHKASMRIGLWVTLFGSIGVLLAGDLQMKALIEGQPMKFAAMEGAYEDSDDPAAWTLIAWADEREHKQVFGIDIPYMLSILSYNKLSGSVDGMDTVNKRLVEKYGDDKNYYPPVNTLFWSFRIMAGFGALMLLVAALGLFFTRKKKPSLYEKKWMLWIVALCTFAPFLANTTGWLVTELGRYPWTVYGLFTIEQSVSPNVSVASLITSNVIYFLLFAGLGSVMVYLVILELRKGPDYEAKKLAKENEPALDPFDKGVFGE
>NZ_SRYT01000001.1|WP_002379582.1|524846_525860_-|cytochrome-d-ubiquinol-oxidase-subunit-II
MSTLQLLWFVLIGVLFSGFFFLEGFDFGVGMAVQTLAHNDDEKDQVVATIGPVWDGNEVWLLTAGGAMFASFPYWYASLFSGYYLILFTILFGLIIRGVSFEFRHNMPEGKRRRMWNWTLSIGSFLVPFFFGILFISLVQGMPLDANGNMHAQFTDYFNLFSIVGGVALTLLCYLHGMNYIALKTEGPIRERARNYAEILYGVLYIGLVVFAVLMYFKTDFYEKNFAVTLILTLAIVVLTVIANVGVFKRKEMLAFLASGLTLVVLVALLFSGLFPRVMIGSEGFDLLIKDATSTPYTLKIMTWISLSILPFVLAYTAWSYYIFRKRISQTAVPEGY
>NZ_SRYT01000001.1|WP_002378477.1|523108_524842_-|thiol-reductant-ABC-exporter-subunit-CydD
MIDKDILQMPKIKKILVLLAGFSFLQAVFIIGQAFFLSKAIVGLWSGGHLNQQLQSILLFFIFYLGRHVITYFRERMLDTFSYERSKELREQLLTKIFRLGPNVVQKNGTGNMVTMALEGISQTENYLQLILTKMMNMMIIPWIILAFVFTQDIRSGITLLIVFPIIIIFMIVLGYAAQSKADKQYAAFQMLSNHFIDSLRGIDTLKLFGLSKKYAGSIYHTSERFRKATMSTLKIAILSTFALDFFTTLSVAVVAVFLGLSLLNGTILLFPALVTLILAPEFFLPIRDFSSDYHATLDGKNSFQAIQAVLALPEAEQTDVLTLDDWHQESQLSVTALNFSYEDATQEAIQSLQFSVDGRKKIGIIGASGSGKSTLINLLSGFLLPTEETSQLAINGQTIPHFLQKDWQKQILYIPQAPYIFQDTLANNIRFYTPEATDEAIQQAIQLVGLDELVKDLPEGIHTLIGESGRMLSGGQAQRVALARAFVDQKRHVLLFDEPTAHLDIETEVEMKERMLPLMNNHLVFFATHRLHWMEEMDYILVMEKGQLVEQGTLAELIEKDGYYVQLMKQMRGGRQ
>NZ_SRYT01000001.1|WP_002379581.1|521345_523112_-|thiol-reductant-ABC-exporter-subunit-CydC
MMEKQPTTKDVWKNDQWVRPFLKRYKKTLYFALLLGFLTFFSAGALMFTSGYLISRAASLPENILLIYIPIVLTRAFGIGRPVFRYVERLTSHNWVLKMTSDLRLKLYNVLEKDAIFFKTKYRTGDILGLLSEDINHIQNLYLRTIFPTVIAWILYIFLVIALGFFSWWFALCMLLMLGVVVFLLPLVSVLVNGARQEKHKYAKNELYQTLTDNILGVSDWVFSQRGSEFVARYETDEANVRALDEKMKQFNRGRDFVLQLLFGVIAIAVLAWTSVRFPGNHGGAANWIGAFVLTVFPLIDAFAPLPAAAQETTIYKDSIRRFNELPEGEDDSTEAPVQPNGTSLSIEHLSFAYENQAKKVLNDLSLTIPEKQKLAILGRSGSGKSTLASLIRGDLRPTSGEILLGNIPTEAFGETMTEYIGVMHQAPYLFRTTILNNIRIGREEASEAEVWAVLEKVGLKEMVAQLPEGLQTMVDEAGLRFSGGERHRLALARILLQDTPIVLLDEPTTGLDPITEQQLLETFFEALKDKTVIWITHHLQGVTLMDQVIFIEDGQLEMSGTPEELLATNAHYQKLYRIDRGISSFEE
>NZ_SRYT01000001.1|WP_002360015.1|520285_521266_+|polyprenyl-synthetase-family-protein
MNTFWKSFPDVERSLTETCELIEQTLHTRNEDIEEALVTLARSGGKLLRPAFFFLFAQLGDEEKQEKQQLLKIAASLEILHMATLIHDDIIDDSPLRRGAVTIQSQYGKDVAVYTGDLLFTEFFTLIADAMNGSEFMKINAQGMKRLLLGELDQMSHRFDRRMSIPAYLRSVNGKTAELFSLSCLEGAYFGHSSKEVQRLAKRIGRHIGIAFQVYDDILDYTADTETLKKPALEDLSQGVYTLPLLFAYQAAPDVFSPYLDKGRAITLEEAAEVAALVNDYHGVTEAQAFAKKVTNKAITDIQQLPDGTAKETLLSLTELLLHRSF
>NZ_SRYT01000001.1|WP_002360016.1|519258_520164_-|prenyltransferase
MNREIFFELVELKAKTASVLPFLLGICFSWYQYGRLHVGYVLIFFIAMFIFNMAVDILDNYNDYHHATEVHDYKEKTNIIGRENLSLPLIRKMIFWMILVSALMGIGLSFIVGWPLLIMGLYCYLIGIFYSSGPKPLSSLPLGEVFSGFTMGFMITLICVYINTFEVFNWSAANLWGIFLVALPNTCYIANLMLANNICDLEEDENNKRYTLVHYLGKASSLKLFVGLNTIAMLAILLAVGLGLTPPTMLLMLLTLPFVRKQTQALLKEQVKSKTFVCAVKILAVGATAQVLFFAIGLWWL
>NZ_SRYT01000001.1|WP_002379580.1|518018_519230_-|NAD(P)/FAD-dependent-oxidoreductase
MNKKHVVILGAGYAGLKTLRELQKGAKDVEITLVDRNNYHYEATDLHEVAAGTQPAEKITYNIMDVVDEKMTTFIQGTVKTIDAATQTVALEDGQTINYDYLVVSLGFESESFGIPGVQEHALQMVDVKTALNVYEHIQEQMRQYKATQNEEFLKIVVCGAGFTGIELLGSLFENKPKFAEIAGVSADQIQIYCVEAVTRLLPMFNEKLANYGVQLLKDSAIHLLLGKPIKEIKPGAVVYQDNEAGDLAELSAKTIIWTTGVSGSHVVGDSGFEARRGRVMVQPDLTDANHSNVYIIGDCSAVMDTETNRPYPTTAQIALKMGAHAAKNIQAQLKGEATKPFSFKSQGSVCSVGNTRALGIVGKTDIKGYPASFMKKIIMNKALFETGGTKEMMAKGRFDLYH
>NZ_SRYT01000001.1|WP_002379579.1|515281_517705_-|DNA-translocase-FtsK
MAQKRSTNKKKSTTKKKTKKQQQQQERLNYMFLGLIFILFGVFGLFRLGFLGTLLANCLRLVVGNTFPFAAILLILYGLLVMIYGKDFPLKRGRPIFGAVLIYISVLLFFHAFMFRNVSGSQPDILGNTWEFLQSDLKANQVTQNVGGGMIGALLYQGTYFLVAQFGSYLIATLLLLAGIFLMSMWDFQQIVDHFQSIQDRLSHVSAKSQARQEEKEAKRAAKKEAKAAERQAKIEAAAQQKLQERERMEQAAAERLTKTPVETHQPMVEEPAAPTPVQIDSFQQQNQAMPVPPIAATKPQREQEEEAADEAGVLEFEISEEAEDRDYQLPPTDLLDTIQATDQSGEYEKIEKNIGVLEQTFKSFGVDAKVVKASLGPSVTKFEVQPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNSAISTVSFRDIVEAQPSHPDKLLEVPLGRDISGMVQTADLSKMPHLLIAGSTGSGKSVAINGIITGILMQAKPHEVKLMMIDPKMVELNVYNGIPHLLTPVVTNPRKAAQALQKVVQEMEFRYEKFAATGVRNITGYNQLIQQKTAEDGENRPILPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDTNGAEKLLGRGDMLFLPMGENKPIRIQGAFISDQEVERVVAFVTDQQEAEYQESMMPTDEPTTSGGGEAPQDELFEEAKNLVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVIGPSEGSKPRKVFLQAESEEAATETPEQ
>NZ_SRYT01000001.1|WP_002356983.1|514450_515152_-|GntR-family-transcriptional-regulator
MRRKSVLYLEVADQIKEDILSGKYPVGTFLPTETELEEMFNVSKITIRRAIEMLATEEFVEKKSGRGTTVLSNRPYNKLSKAGTFTEFLNESGQKVTKKVLQVENLTLSKEMPAYQFLGEEVVHLSRLYLIDEQPYIYFNYYLPAAMKDVSLTEYKEESLYRLMDRHAIEIYKFEDRFEVATLTEAEQKMLQTTETTGLKRIRRSLSPTGRCVEYSEAIYNTKIHPYVIEYEA
>NZ_SRYT01000001.1|WP_002381711.1|513583_514333_-|ABC-transporter-ATP-binding-protein
MLEVQNLKKVYGNEIKYEALKGINLTVQDGEFIGIMGPSGSGKSTLLNLLATIDSPTDGEILLNGKNPNNLNQEQIAKFRRTELGFVFQSFNLMPTLTVEENIILPLTLDGEKVSVMKRQLAELSERLGINHLLKKRIAEISGGQAQRVAVARAMIHHPQLLLADEPTGNLDTKSSKDVMGLLQQLNEEEAATILMVTHDPLAASYCKRIVFIKDGELIDEIVQNGNQKEFYDLIMVKLAEIEGVDNEF
>NZ_SRYT01000001.1|WP_002379583.1|528138_529002_-|AraC-family-transcriptional-regulator
MLANLNQAMDYIEEHLTEEVSFDELAKKTGISVYHFKRTFSFITGMSLAEYIKKRPLAEANLALLAGEKVTDVAFKYGYQSIEGFSRAFRDWSGQAPSEVMKTQIQKTFPKFSFYIDIKGGQSMDVKLIEKPAFQIVGVSQKVPLQYQGENQAIMELAQRITPQQRAEMHTFDDIYPHQVVNASFDFQEGRTTEGGEMTHMIGFATSQENTYEDLEQLSVPAHTWAVFPNEGPFPQTLQETWAKIFSEWLPSSGYQVVAAPEISFTQYQGPAEAKYSEIWLAVTATK
>NZ_SRYT01000001.1|WP_002362072.1|529086_531168_-|DNA-topoisomerase-III
MVAKQLIIAEKPSVAKDLSRVLGANQKNKNYYEGPNVIVTWALGHLLGLKMPEDLNKEWQTWQMETLPMIPKNLGIKPLPKTGHQLKAIKQLANRKDVSEAVIATDAGREGELVARWILEYVRFNKPVKRLWISSQTDKAIKDGFKKIRPAKDYDNLYYSALARAKADWLVGLNVTRALTVKYQDNLSAGRVQTPTLAMVRQQEKTIEQFKPQTYFTISLSVESEKAKMTQKNPYALKERQEAEQLVKELSKQKGLVTDIQEKVKTENAPLPYDLTEIQREANQRFQFSAKKTLSLVQSLYETHKIVSYPRTDSKYLTTDMKGTMKERLQAIADFSPEVKGYLKNGAVVKQQKVFQNAKVTDHHGLIPTEQRPRYEKLSNDEQKIYQMIVQRFLGLFAEPNQTKQTKVTVAFGKETFVFHQNKVVVAGWKTTAEQPLSTVQWQKGMTVAPNFTINKELTSPPKPLTEGTLLGKMEKHSLGTPATRAEIIEKLIKSELMERTNSGLSVSAKGKQLLDLVNPSLVTPELTEKWEKSLEAIASGQQKSQLFLKDIEEDTKKLVREIKQSEKKYQDFSITQKKCPDCGSNLREKNTKDGKIYVCTNQECSYRRRKDPKVSNHRCPQCHKKMVIIEGKNGRSFKCKFCSITEKIPDKKERKQKMTKHEERRLMKKYSQPDEPEESALAQALKAAMNQE
>NZ_SRYT01000001.1|WP_002356966.1|531298_531664_-|DUF1033-family-protein
MYQVITMYGDNEPWWFFDDWQEDIVQEKTFDNLTDAETYYVEVWQKLSQNYTYVNTKPNYLTAFWNDGEERWCEECDEDLQQYFGLALLENHHAVTFENKAEFTTISNHFGKTKFCKRKVI
>NZ_SRYT01000001.1|WP_002379584.1|531761_532331_-|TetR/AcrR-family-transcriptional-regulator
MAKKRNLTKAKILESARNLAEELGVQQLTFQNLAVDLGIKYPSLYNHFKNIAEVKNALVDLLIQELNDALRRALVGKSGAEAIRIYAETYQQFAFENSAVYELLISVPKTQNQQLIEGIHETNQIILQLLAFYPFNNEERLHKSRELRSLIHGYITLRFLGYFQREEATPEESYRRMIEDFIVSLEVAG
>NZ_SRYT01000001.1|WP_002379586.1|532463_532979_+|YbhB/YbcL-family-Raf-kinase-inhibitor-like-protein
MHITNRSHLADRYGKRTSAEFLLHERPILSFPISLAAIPKEARYLSWELIDYDTIPLIGFPFIHWLAANVPVTNEIPEDFSRTALKPTIQGKNSLASPLGKQMIPALATETLPELTERYLGLSPRSGVHTYTLYVYTTKNPLNLKEGFFANELHQALNDQLLAKAKFEFLY
>NZ_SRYT01000001.1|WP_002362074.1|532995_534234_+|multidrug-efflux-MFS-transporter
MYELIKSKIFRPRQPWEKNLLVLWFGTFMAGIGFSLVMPFMPLYINTLGTFTHQQLNFWSGITFSSTFLVTTIVSPWWGRLADRKGRKLMLLRASLGMAIVISLMGAVTSVYQLIGLRLLQGVFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTLLGPLLGGVTASIFGYRPTFFITGTILLLVFVLSLVFVHEEFVPIEKNQAASGKQILKKLEHPHVILGMFITTLIIQASNNSISPIISLYIQQLLGGHGNVTLISGVIASIPGIATLIAAPRFGRLGDRIGSERILTIGLILAIFVYLPMAFVQNVWQLAMLRFLVGISDACLLPAVQTLITRYSPSDAAGRIFSYNQSFQATGNVIGPMIGSSVSAAFGYRGVFISTSCLVLLNLLWVRRSTAELKKEKNDD
>NZ_SRYT01000001.1|WP_002362075.1|534413_535538_-|tRNA-2-thiouridine(34)-synthase-MnmA
MADNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDTDENGVCTATEDYKDVAKVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRLGRTPNPDVMCNKEIKFKAFLDYAMQLGADYVATGHYAQVETDENGVVHMLRGIDNNKDQTYFLSQLSQAQLAKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGVCFIGEKNFKEFLSNYLPAKKGNMVTEDGEIKGQHDGLMYYTIGQRQGLGIGGGGKTQEPWFVIGKDLTTNTLYVGQGFHHEKLYATHLEASEVHFTVDTPMPKEFDCTAKFRYRQADIPVHVSLSEDGTKATVTFKEPARAVTPGQAVVFYDGMECLGGGLIDRAYQDEKELQYV
>NZ_SRYT01000001.1|WP_002356959.1|535763_536108_-|DUF1831-domain-containing-protein
MAFTTTAAVEGSTVFYKVHPSAKRYTLKDNGFTETKSGNFQLIRSLDPTPQRNEGFKLKITITADLKELKMSITTANGLKPMNIFKNEQHEMSKEKYFFLMDGLISRGVLEKVE
>NZ_SRYT01000001.1|WP_087548645.1|536175_537321_-|cysteine-desulfurase
MEPIYLDHAATTPLHPTVIQAMTENMQTTFGNPSSIHQFGRKAHGLLEEVRQTIAESLQAKPHEIIFNSGGTEGDNTAILAVAFSRQKEGKHIITTAIEHPAVLRTMEYLETLGFEVTYLPVNENGQISMDQFKKSLREETILVSMMYGNNEIGNRLPIAEVGAILKNHLAIFHTDAVQAYGSEVILPHELGIDLLSISAHKINGPKGVGFLFKSDAIQLPPLLHGGEQEEKRRAGTENLAGIIGMGTAVSLLTSAEKQARKTAYQSFQTIILKALEEANIDFSINGEPTNRLAHVLNLHLKGIPSDLLLMHLDLRGIAISTGSACTAGTVDPSHVLTAMYGENSSAIKESIRISFGYGNTPEEIATFSEVLVAVIQQLKK
>NZ_SRYT01000001.1|WP_002379587.1|537477_538452_-|ribose-phosphate-diphosphokinase
MTENYQDDTLRIFSLNANRPLAEKIAASVGTELGKSTVRQFSDGEIQINIEESIRGDHVYIIQATNAPVNDHLMELLILIDALKRASAKTVNVILPYYGYARQDRTAKPREPITAKLVANMLVEAGATRLLTLDLHTVQVQGFFDIPVDNLFTMPLFAHYYRQQALVGEEIVIVSPKNSGVQRARSLSEYLDATLAIVDHEEIDGVRQEGYVIGNVAGKKCILVDDILNTGQTLATAAEVLMKNGAQEVYACASHGLLSEGAKATLENAPIKEISITDSVYTTADRQPATLNIISCAELMGEALLRIHENKPMSPLFRLEPKGE

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Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity
NZ_SRYT01000001_1	1.7\|270261\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270261-270290	30	MH375074	Enterococcus phage LY0323, complete genome	14191-14220	0	1.0
NZ_SRYT01000001_1	1.7\|270261\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270261-270290	30	MH355583	Enterococcus phage vB_EfaS_LM99, complete genome	25256-25285	0	1.0
NZ_SRYT01000001_1	1.7\|270261\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270261-270290	30	MK721199	Enterococcus phage vB_EfaS_Ef5.1, complete genome	25294-25323	0	1.0
NZ_SRYT01000001_1	1.7\|270261\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270261-270290	30	MK360024	Enterococcus phage vB_EfaS_Max, complete genome	26997-27026	0	1.0
NZ_SRYT01000001_1	1.7\|270261\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270261-270290	30	CAJDJX010000002	Enterococcus phage Q69 genome assembly, contig: phageQ69-genome, whole genome shotgun sequence	28839-28868	0	1.0
NZ_SRYT01000001_1	1.7\|270261\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270261-270290	30	MT857001	Enterococcus phage EFA1, complete genome	26140-26169	0	1.0
NZ_SRYT01000001_1	1.7\|270261\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270261-270290	30	KT932701	Enterococcus phage vB_EfaS_IME196, complete genome	25559-25588	0	1.0
NZ_SRYT01000001_1	1.7\|270261\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270261-270290	30	NC_042023	Enterococcus phage phiSHEF5, complete genome	15973-16002	0	1.0
NZ_SRYT01000001_1	1.7\|270261\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270261-270290	30	NC_042101	Enterococcus phage PMBT2, complete genome	26219-26248	0	1.0
NZ_SRYT01000001_1	1.7\|270261\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270261-270290	30	MH203383	Ecterococcus phage vB_EfaS_AL3, complete genome	27301-27330	0	1.0
NZ_SRYT01000001_1	1.7\|270261\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270261-270290	30	MK721200	Enterococcus phage vB_EfaS_Ef5.3, complete genome	25586-25615	0	1.0
NZ_SRYT01000001_1	1.7\|270261\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270261-270290	30	NC_042022	Enterococcus phage phiSHEF4, complete genome	19571-19600	0	1.0
NZ_SRYT01000001_1	1.7\|270261\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270261-270290	30	MT520979	Enterococcus phage vB_EfaS-271, complete genome	27170-27199	0	1.0
NZ_SRYT01000001_1	1.7\|270261\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270261-270290	30	MH193369	Enterococcus phage LY0322, complete genome	13774-13803	0	1.0
NZ_SRYT01000001_1	1.7\|270261\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270261-270290	30	NC_031260	Enterococcus phage Ec-ZZ2, complete genome	4183-4212	0	1.0
NZ_SRYT01000001_1	1.7\|270261\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270261-270290	30	MK721186	Enterococcus phage vB_EfaS_Ef5.2, complete genome	25777-25806	0	1.0
NZ_SRYT01000001_1	1.7\|270261\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270261-270290	30	NC_042021	Enterococcus phage phiSHEF2, complete genome	26175-26204	0	1.0
NZ_SRYT01000001_1	1.7\|270261\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270261-270290	30	JX193904	Enterococcus phage EfaCPT1, complete genome	24158-24187	0	1.0
NZ_SRYT01000001_1	1.7\|270261\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270261-270290	30	NC_042126	Enterococcus phage vB_EfaS_AL3, complete genome	27301-27330	0	1.0
NZ_SRYT01000001_1	1.7\|270261\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270261-270290	30	KX284704	Enterococcus phage SANTOR1, complete genome	23374-23403	0	1.0
NZ_SRYT01000001_1	1.7\|270261\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270261-270290	30	KJ127304	Enterococcus phage AUEF3, partial genome	16779-16808	0	1.0
NZ_SRYT01000001_2	2.1\|527718\|46\|NZ_SRYT01000001\|CRT	527718-527763	46	MF157411	Escherichia coli strain EC1000 plasmid pCR2, complete sequence	288-333	0	1.0
NZ_SRYT01000001_2	2.2\|527784\|46\|NZ_SRYT01000001\|CRT	527784-527829	46	MF157411	Escherichia coli strain EC1000 plasmid pCR2, complete sequence	222-267	0	1.0
NZ_SRYT01000001_2	2.5\|527785\|27\|NZ_SRYT01000001\|CRISPRCasFinder	527785-527811	27	MF157411	Escherichia coli strain EC1000 plasmid pCR2, complete sequence	240-266	0	1.0
NZ_SRYT01000001_1	1.7\|270261\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270261-270290	30	KF728385	Enterococcus phage IME_EF3, complete genome	27212-27241	1	0.967
NZ_SRYT01000001_1	1.7\|270261\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270261-270290	30	MK721190	Enterococcus phage vB_EfaS_Ef6.4, complete genome	25243-25272	1	0.967
NZ_SRYT01000001_1	1.7\|270261\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270261-270290	30	NC_042127	Enterococcus phage vB_EfaS_AL2, complete genome	14027-14056	1	0.967
NZ_SRYT01000001_1	1.7\|270261\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270261-270290	30	MG264739	UNVERIFIED: Enterococcus phage phiNASRA1, complete genome	2703-2732	1	0.967
NZ_SRYT01000001_1	1.7\|270261\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270261-270290	30	CAJDKF010000002	Enterococcus phage vB_EfaS_159 genome assembly, contig: phage159-genome, whole genome shotgun sequence	28650-28679	1	0.967
NZ_SRYT01000001_1	1.7\|270261\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270261-270290	30	KF733017	Enterococcus phage IME-EF4, complete genome	14831-14860	1	0.967
NZ_SRYT01000001_1	1.7\|270261\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270261-270290	30	MK721191	Enterococcus phage vB_EfaS_Ef5.4, complete genome	25476-25505	2	0.933
NZ_SRYT01000001_1	1.7\|270261\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270261-270290	30	MK721187	Enterococcus phage vB_EfaS_Ef6.1, complete genome	25547-25576	2	0.933
NZ_SRYT01000001_1	1.7\|270261\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270261-270290	30	MK982307	Enterococcus phage MSF2, complete genome	32076-32105	3	0.9
NZ_SRYT01000001_1	1.4\|270063\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270063-270092	30	LT615366	Enterococcus phage VPE25 genome assembly, chromosome: VPE25	28752-28781	6	0.8
NZ_SRYT01000001_1	1.4\|270063\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270063-270092	30	LT546029	Enterococcus phage VFW genome assembly, chromosome: VFW	28684-28713	6	0.8
NZ_SRYT01000001_1	1.4\|270063\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270063-270092	30	LT546030	Enterococcus phage VPE25 genome assembly, chromosome: I	28752-28781	6	0.8
NZ_SRYT01000001_1	1.4\|270063\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270063-270092	30	MT119361	Enterococus phage vipetofem, complete genome	56526-56555	6	0.8
NZ_SRYT01000001_1	1.6\|270195\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270195-270224	30	MG592415	Vibrio phage 1.031.O._10N.261.46.F8, partial genome	10873-10902	6	0.8
NZ_SRYT01000001_1	1.6\|270195\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270195-270224	30	NZ_CP054613	Paenibacillus cellulosilyticus strain KACC 14175 plasmid unnamed4, complete sequence	1590971-1591000	6	0.8
NZ_SRYT01000001_2	2.5\|527785\|27\|NZ_SRYT01000001\|CRISPRCasFinder	527785-527811	27	NC_018746	Pseudomonas putida ND6 plasmid pND6-2, complete sequence	111387-111413	6	0.778
NZ_SRYT01000001_1	1.4\|270063\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270063-270092	30	NZ_CP041427	Escherichia coli strain STEC388 plasmid pSTEC388_2, complete sequence	56283-56312	7	0.767
NZ_SRYT01000001_1	1.4\|270063\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270063-270092	30	NZ_CP011362	Salimicrobium jeotgali strain MJ3 plasmid pSJ52, complete sequence	1949-1978	7	0.767
NZ_SRYT01000001_1	1.4\|270063\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270063-270092	30	CP006767	Lactococcus lactis subsp. lactis KLDS 4.0325 plasmid unnamed1, complete sequence	408-437	7	0.767
NZ_SRYT01000001_1	1.4\|270063\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270063-270092	30	NZ_CP044979	Bacillus thuringiensis strain BT62 plasmid pBT62A, complete sequence	481058-481087	7	0.767
NZ_SRYT01000001_1	1.4\|270063\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270063-270092	30	NC_004922	Lactococcus lactis lactis BGMN1-5 plasmid pMN5, complete sequence	763-792	7	0.767
NZ_SRYT01000001_1	1.4\|270063\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270063-270092	30	NZ_CP038659	Citrobacter freundii strain 680 plasmid p680_1, complete sequence	363776-363805	7	0.767
NZ_SRYT01000001_1	1.4\|270063\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270063-270092	30	NZ_CP016589	Bacillus thuringiensis strain KNU-07 plasmid pBTKNU07-01, complete sequence	106851-106880	7	0.767
NZ_SRYT01000001_1	1.5\|270129\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270129-270158	30	MN794232	Vibrio phage VH1_2019, complete genome	117004-117033	7	0.767
NZ_SRYT01000001_1	1.5\|270129\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270129-270158	30	NC_005083	Vibrio phage KVP40, complete genome	116465-116494	7	0.767
NZ_SRYT01000001_1	1.5\|270129\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270129-270158	30	JN849462	Vibriophage phi-pp2, complete genome	116408-116437	7	0.767
NZ_SRYT01000001_1	1.5\|270129\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270129-270158	30	MT135024	Vibrio phage V05, complete genome	59483-59512	7	0.767
NZ_SRYT01000001_1	1.5\|270129\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270129-270158	30	AY283928	Bacteriophage KVP40, complete genome	116465-116494	7	0.767
NZ_SRYT01000001_1	1.5\|270129\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270129-270158	30	MT135026	Vibrio phage V09, complete genome	227856-227885	7	0.767
NZ_SRYT01000001_1	1.5\|270129\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270129-270158	30	MT135025	Vibrio phage V07, complete genome	164567-164596	7	0.767
NZ_SRYT01000001_1	1.3\|269997\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	269997-270026	30	MN176217	Escherichia phage vB_Eco4M-7, complete genome	54013-54042	8	0.733
NZ_SRYT01000001_1	1.3\|269997\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	269997-270026	30	MH051335	Escherichia phage vB_EcoM-Ro157lw, complete genome	47776-47805	8	0.733
NZ_SRYT01000001_1	1.3\|269997\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	269997-270026	30	JX128258	Escherichia phage ECML-117, complete genome	52688-52717	8	0.733
NZ_SRYT01000001_1	1.3\|269997\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	269997-270026	30	NC_048194	Escherichia phage vB_EcoM_WFH, complete genome	47826-47855	8	0.733
NZ_SRYT01000001_1	1.3\|269997\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	269997-270026	30	MT446405	UNVERIFIED: Escherichia virus TH32, complete genome	47325-47354	8	0.733
NZ_SRYT01000001_1	1.3\|269997\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	269997-270026	30	MT446408	UNVERIFIED: Escherichia virus TH35, complete genome	8122-8151	8	0.733
NZ_SRYT01000001_1	1.3\|269997\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	269997-270026	30	NC_048195	Escherichia phage vB_EcoM_WFC, complete genome	47764-47793	8	0.733
NZ_SRYT01000001_1	1.3\|269997\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	269997-270026	30	NC_048073	Escherichia phage FEC19, complete genome	22675-22704	8	0.733
NZ_SRYT01000001_1	1.3\|269997\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	269997-270026	30	MT684590	Streptomyces phage LilMartin, complete genome	63102-63131	8	0.733
NZ_SRYT01000001_1	1.3\|269997\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	269997-270026	30	MT897905	Streptomyces phage MulchMansion, complete genome	63102-63131	8	0.733
NZ_SRYT01000001_1	1.4\|270063\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270063-270092	30	MN693967	Marine virus AFVG_250M992, complete genome	40288-40317	8	0.733
NZ_SRYT01000001_1	1.4\|270063\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270063-270092	30	NZ_CP040783	Bacillus thuringiensis strain HM-311 plasmid p1, complete sequence	100498-100527	8	0.733
NZ_SRYT01000001_1	1.4\|270063\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270063-270092	30	NZ_CP014283	Bacillus thuringiensis strain Bt185 plasmid pBT1850636, complete sequence	303135-303164	8	0.733
NZ_SRYT01000001_1	1.4\|270063\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270063-270092	30	NZ_CP032614	Bacillus thuringiensis strain QZL38 plasmid p.1, complete sequence	476724-476753	8	0.733
NZ_SRYT01000001_1	1.4\|270063\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270063-270092	30	CP046512	Bacillus cereus strain JHU plasmid p1, complete sequence	475721-475750	8	0.733
NZ_SRYT01000001_1	1.4\|270063\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270063-270092	30	NZ_CP011154	Bacillus cereus strain CMCC P0011 plasmid pRML05, complete sequence	336359-336388	8	0.733
NZ_SRYT01000001_1	1.4\|270063\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270063-270092	30	NZ_CP011152	Bacillus cereus strain CMCC P0021 plasmid pRML04, complete sequence	210527-210556	8	0.733
NZ_SRYT01000001_1	1.4\|270063\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270063-270092	30	NZ_CP045607	Bacillus cereus strain SB1 plasmid p1, complete sequence	508466-508495	8	0.733
NZ_SRYT01000001_1	1.4\|270063\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270063-270092	30	NZ_CP050184	Bacillus thuringiensis strain HER1410 plasmid pLUSID1, complete sequence	294951-294980	8	0.733
NZ_SRYT01000001_1	1.4\|270063\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270063-270092	30	NC_015583	Novosphingobium sp. PP1Y plasmid Mpl, complete sequence	1124729-1124758	8	0.733
NZ_SRYT01000001_1	1.5\|270129\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270129-270158	30	KP671755	Vibrio phage ValKK3, complete genome	10086-10115	8	0.733
NZ_SRYT01000001_1	1.6\|270195\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270195-270224	30	NZ_CP049908	Hymenobacter sp. HDW8 plasmid p_unnamed1, complete sequence	280146-280175	8	0.733
NZ_SRYT01000001_1	1.4\|270063\|30\|NZ_SRYT01000001\|PILER-CR,CRISPRCasFinder,CRT	270063-270092	30	CP040041	Acinetobacter baumannii strain VB958 plasmid unnamed1, complete sequence	530179-530208	9	0.7

1. spacer 1.7|270261|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to MH375074 (Enterococcus phage LY0323, complete genome) position: , mismatch: 0, identity: 1.0

ttacaccaaagcgaaacatattcaactttt	CRISPR spacer
ttacaccaaagcgaaacatattcaactttt	Protospacer
******************************

2. spacer 1.7|270261|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to MH355583 (Enterococcus phage vB_EfaS_LM99, complete genome) position: , mismatch: 0, identity: 1.0

ttacaccaaagcgaaacatattcaactttt	CRISPR spacer
ttacaccaaagcgaaacatattcaactttt	Protospacer
******************************

3. spacer 1.7|270261|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to MK721199 (Enterococcus phage vB_EfaS_Ef5.1, complete genome) position: , mismatch: 0, identity: 1.0

ttacaccaaagcgaaacatattcaactttt	CRISPR spacer
ttacaccaaagcgaaacatattcaactttt	Protospacer
******************************

4. spacer 1.7|270261|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to MK360024 (Enterococcus phage vB_EfaS_Max, complete genome) position: , mismatch: 0, identity: 1.0

ttacaccaaagcgaaacatattcaactttt	CRISPR spacer
ttacaccaaagcgaaacatattcaactttt	Protospacer
******************************

5. spacer 1.7|270261|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to CAJDJX010000002 (Enterococcus phage Q69 genome assembly, contig: phageQ69-genome, whole genome shotgun sequence) position: , mismatch: 0, identity: 1.0

ttacaccaaagcgaaacatattcaactttt	CRISPR spacer
ttacaccaaagcgaaacatattcaactttt	Protospacer
******************************

6. spacer 1.7|270261|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to MT857001 (Enterococcus phage EFA1, complete genome) position: , mismatch: 0, identity: 1.0

ttacaccaaagcgaaacatattcaactttt	CRISPR spacer
ttacaccaaagcgaaacatattcaactttt	Protospacer
******************************

7. spacer 1.7|270261|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to KT932701 (Enterococcus phage vB_EfaS_IME196, complete genome) position: , mismatch: 0, identity: 1.0

ttacaccaaagcgaaacatattcaactttt	CRISPR spacer
ttacaccaaagcgaaacatattcaactttt	Protospacer
******************************

8. spacer 1.7|270261|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to NC_042023 (Enterococcus phage phiSHEF5, complete genome) position: , mismatch: 0, identity: 1.0

ttacaccaaagcgaaacatattcaactttt	CRISPR spacer
ttacaccaaagcgaaacatattcaactttt	Protospacer
******************************

9. spacer 1.7|270261|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to NC_042101 (Enterococcus phage PMBT2, complete genome) position: , mismatch: 0, identity: 1.0

ttacaccaaagcgaaacatattcaactttt	CRISPR spacer
ttacaccaaagcgaaacatattcaactttt	Protospacer
******************************

10. spacer 1.7|270261|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to MH203383 (Ecterococcus phage vB_EfaS_AL3, complete genome) position: , mismatch: 0, identity: 1.0

ttacaccaaagcgaaacatattcaactttt	CRISPR spacer
ttacaccaaagcgaaacatattcaactttt	Protospacer
******************************

11. spacer 1.7|270261|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to MK721200 (Enterococcus phage vB_EfaS_Ef5.3, complete genome) position: , mismatch: 0, identity: 1.0

ttacaccaaagcgaaacatattcaactttt	CRISPR spacer
ttacaccaaagcgaaacatattcaactttt	Protospacer
******************************

12. spacer 1.7|270261|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to NC_042022 (Enterococcus phage phiSHEF4, complete genome) position: , mismatch: 0, identity: 1.0

ttacaccaaagcgaaacatattcaactttt	CRISPR spacer
ttacaccaaagcgaaacatattcaactttt	Protospacer
******************************

13. spacer 1.7|270261|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to MT520979 (Enterococcus phage vB_EfaS-271, complete genome) position: , mismatch: 0, identity: 1.0

ttacaccaaagcgaaacatattcaactttt	CRISPR spacer
ttacaccaaagcgaaacatattcaactttt	Protospacer
******************************

14. spacer 1.7|270261|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to MH193369 (Enterococcus phage LY0322, complete genome) position: , mismatch: 0, identity: 1.0

ttacaccaaagcgaaacatattcaactttt	CRISPR spacer
ttacaccaaagcgaaacatattcaactttt	Protospacer
******************************

15. spacer 1.7|270261|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to NC_031260 (Enterococcus phage Ec-ZZ2, complete genome) position: , mismatch: 0, identity: 1.0

ttacaccaaagcgaaacatattcaactttt	CRISPR spacer
ttacaccaaagcgaaacatattcaactttt	Protospacer
******************************

16. spacer 1.7|270261|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to MK721186 (Enterococcus phage vB_EfaS_Ef5.2, complete genome) position: , mismatch: 0, identity: 1.0

ttacaccaaagcgaaacatattcaactttt	CRISPR spacer
ttacaccaaagcgaaacatattcaactttt	Protospacer
******************************

17. spacer 1.7|270261|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to NC_042021 (Enterococcus phage phiSHEF2, complete genome) position: , mismatch: 0, identity: 1.0

ttacaccaaagcgaaacatattcaactttt	CRISPR spacer
ttacaccaaagcgaaacatattcaactttt	Protospacer
******************************

18. spacer 1.7|270261|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to JX193904 (Enterococcus phage EfaCPT1, complete genome) position: , mismatch: 0, identity: 1.0

ttacaccaaagcgaaacatattcaactttt	CRISPR spacer
ttacaccaaagcgaaacatattcaactttt	Protospacer
******************************

19. spacer 1.7|270261|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to NC_042126 (Enterococcus phage vB_EfaS_AL3, complete genome) position: , mismatch: 0, identity: 1.0

ttacaccaaagcgaaacatattcaactttt	CRISPR spacer
ttacaccaaagcgaaacatattcaactttt	Protospacer
******************************

20. spacer 1.7|270261|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to KX284704 (Enterococcus phage SANTOR1, complete genome) position: , mismatch: 0, identity: 1.0

ttacaccaaagcgaaacatattcaactttt	CRISPR spacer
ttacaccaaagcgaaacatattcaactttt	Protospacer
******************************

21. spacer 1.7|270261|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to KJ127304 (Enterococcus phage AUEF3, partial genome) position: , mismatch: 0, identity: 1.0

ttacaccaaagcgaaacatattcaactttt	CRISPR spacer
ttacaccaaagcgaaacatattcaactttt	Protospacer
******************************

22. spacer 2.1|527718|46|NZ_SRYT01000001|CRT matches to MF157411 (Escherichia coli strain EC1000 plasmid pCR2, complete sequence) position: , mismatch: 0, identity: 1.0

aggggagaaaaagccaaatcaaaaaagtttgttttggtaccattcc	CRISPR spacer
aggggagaaaaagccaaatcaaaaaagtttgttttggtaccattcc	Protospacer
**********************************************

23. spacer 2.2|527784|46|NZ_SRYT01000001|CRT matches to MF157411 (Escherichia coli strain EC1000 plasmid pCR2, complete sequence) position: , mismatch: 0, identity: 1.0

tatagtcattatgctgaacaagggtttgttgttttggtaccattct	CRISPR spacer
tatagtcattatgctgaacaagggtttgttgttttggtaccattct	Protospacer
**********************************************

24. spacer 2.5|527785|27|NZ_SRYT01000001|CRISPRCasFinder matches to MF157411 (Escherichia coli strain EC1000 plasmid pCR2, complete sequence) position: , mismatch: 0, identity: 1.0

atagtcattatgctgaacaagggtttg	CRISPR spacer
atagtcattatgctgaacaagggtttg	Protospacer
***************************

25. spacer 1.7|270261|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to KF728385 (Enterococcus phage IME_EF3, complete genome) position: , mismatch: 1, identity: 0.967

ttacaccaaagcgaaacatattcaactttt	CRISPR spacer
ctacaccaaagcgaaacatattcaactttt	Protospacer
.*****************************

26. spacer 1.7|270261|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to MK721190 (Enterococcus phage vB_EfaS_Ef6.4, complete genome) position: , mismatch: 1, identity: 0.967

ttacaccaaagcgaaacatattcaactttt	CRISPR spacer
ttgcaccaaagcgaaacatattcaactttt	Protospacer
**.***************************

27. spacer 1.7|270261|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to NC_042127 (Enterococcus phage vB_EfaS_AL2, complete genome) position: , mismatch: 1, identity: 0.967

ttacaccaaagcgaaacatattcaactttt	CRISPR spacer
ttgcaccaaagcgaaacatattcaactttt	Protospacer
**.***************************

28. spacer 1.7|270261|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to MG264739 (UNVERIFIED: Enterococcus phage phiNASRA1, complete genome) position: , mismatch: 1, identity: 0.967

ttacaccaaagcgaaacatattcaactttt	CRISPR spacer
ttacaccaaagcgaaacgtattcaactttt	Protospacer
*****************.************

29. spacer 1.7|270261|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to CAJDKF010000002 (Enterococcus phage vB_EfaS_159 genome assembly, contig: phage159-genome, whole genome shotgun sequence) position: , mismatch: 1, identity: 0.967

ttacaccaaagcgaaacatattcaactttt	CRISPR spacer
ttgcaccaaagcgaaacatattcaactttt	Protospacer
**.***************************

30. spacer 1.7|270261|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to KF733017 (Enterococcus phage IME-EF4, complete genome) position: , mismatch: 1, identity: 0.967

ttacaccaaagcgaaacatattcaactttt	CRISPR spacer
ttgcaccaaagcgaaacatattcaactttt	Protospacer
**.***************************

31. spacer 1.7|270261|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to MK721191 (Enterococcus phage vB_EfaS_Ef5.4, complete genome) position: , mismatch: 2, identity: 0.933

ttacaccaaagcgaaacatattcaactttt	CRISPR spacer
ttacaccaaagcgaaacatattcaagcttt	Protospacer
************************* .***

32. spacer 1.7|270261|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to MK721187 (Enterococcus phage vB_EfaS_Ef6.1, complete genome) position: , mismatch: 2, identity: 0.933

ttacaccaaagcgaaacatattcaactttt	CRISPR spacer
ttacatcagagcgaaacatattcaactttt	Protospacer
*****.**.*********************

33. spacer 1.7|270261|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to MK982307 (Enterococcus phage MSF2, complete genome) position: , mismatch: 3, identity: 0.9

ttacaccaaagcgaaacatattcaactttt	CRISPR spacer
ttgcatcaaagcgaaacatattcaactttc	Protospacer
**.**.***********************.

34. spacer 1.4|270063|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to LT615366 (Enterococcus phage VPE25 genome assembly, chromosome: VPE25) position: , mismatch: 6, identity: 0.8

ctttcaaa----agaaaataattgattcaattga	CRISPR spacer
----taaagtggagaaaataattgatttaattga	Protospacer
    .***    ***************.******

35. spacer 1.4|270063|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to LT546029 (Enterococcus phage VFW genome assembly, chromosome: VFW) position: , mismatch: 6, identity: 0.8

ctttcaaa----agaaaataattgattcaattga	CRISPR spacer
----taaagtggagaaaataattgatttaattga	Protospacer
    .***    ***************.******

36. spacer 1.4|270063|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to LT546030 (Enterococcus phage VPE25 genome assembly, chromosome: I) position: , mismatch: 6, identity: 0.8

ctttcaaa----agaaaataattgattcaattga	CRISPR spacer
----taaagtggagaaaataattgatttaattga	Protospacer
    .***    ***************.******

37. spacer 1.4|270063|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to MT119361 (Enterococus phage vipetofem, complete genome) position: , mismatch: 6, identity: 0.8

ctttcaaa----agaaaataattgattcaattga	CRISPR spacer
----taaagtggagaaaataattgatttaattga	Protospacer
    .***    ***************.******

38. spacer 1.6|270195|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to MG592415 (Vibrio phage 1.031.O._10N.261.46.F8, partial genome) position: , mismatch: 6, identity: 0.8

cactatttggggagacgagcgtgacgagcc	CRISPR spacer
taatttttagggtgacgagcgtgacgagca	Protospacer
.* * ***.*** ****************

39. spacer 1.6|270195|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP054613 (Paenibacillus cellulosilyticus strain KACC 14175 plasmid unnamed4, complete sequence) position: , mismatch: 6, identity: 0.8

cactatttggggagacgagcgtgacgagcc	CRISPR spacer
catcagctggggagacaagcgtgccgagcc	Protospacer
**..* .*********.****** ******

40. spacer 2.5|527785|27|NZ_SRYT01000001|CRISPRCasFinder matches to NC_018746 (Pseudomonas putida ND6 plasmid pND6-2, complete sequence) position: , mismatch: 6, identity: 0.778

atagtcattatgctgaacaagggtttg	CRISPR spacer
gaggtcatgatgccgaacaagggtttc	Protospacer
. .***** ****.************

41. spacer 1.4|270063|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP041427 (Escherichia coli strain STEC388 plasmid pSTEC388_2, complete sequence) position: , mismatch: 7, identity: 0.767

ctttcaaaagaaaataattgattcaattga	CRISPR spacer
tccacaaaagaaaataatttatttaattca	Protospacer
... *************** ***.**** *

42. spacer 1.4|270063|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP011362 (Salimicrobium jeotgali strain MJ3 plasmid pSJ52, complete sequence) position: , mismatch: 7, identity: 0.767

ctttcaaaagaaaataattgattcaattga	CRISPR spacer
ggctgaaaagaaaatatttaattcaattgg	Protospacer
  .* *********** **.*********.

43. spacer 1.4|270063|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to CP006767 (Lactococcus lactis subsp. lactis KLDS 4.0325 plasmid unnamed1, complete sequence) position: , mismatch: 7, identity: 0.767

---ctttcaaaagaaaataattgattcaattga	CRISPR spacer
aaatttt---aagaaaattattgattcaattag	Protospacer
   .***   ******** ************..

44. spacer 1.4|270063|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP044979 (Bacillus thuringiensis strain BT62 plasmid pBT62A, complete sequence) position: , mismatch: 7, identity: 0.767

ctttcaaaagaaaataattgattcaattga	CRISPR spacer
ctttcaaaagaaaaaaattaattaacacgt	Protospacer
************** ****.*** *  .*

45. spacer 1.4|270063|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to NC_004922 (Lactococcus lactis lactis BGMN1-5 plasmid pMN5, complete sequence) position: , mismatch: 7, identity: 0.767

---ctttcaaaagaaaataattgattcaattga	CRISPR spacer
aaatttt---aagaaaattattgattcaattag	Protospacer
   .***   ******** ************..

46. spacer 1.4|270063|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP038659 (Citrobacter freundii strain 680 plasmid p680_1, complete sequence) position: , mismatch: 7, identity: 0.767

ctttcaaaagaaaataattgattcaattga	CRISPR spacer
atttcaaaagaaattaattgatgctgatta	Protospacer
 ************ ******** * . * *

47. spacer 1.4|270063|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP016589 (Bacillus thuringiensis strain KNU-07 plasmid pBTKNU07-01, complete sequence) position: , mismatch: 7, identity: 0.767

ctttcaaaagaaaataattgattcaattga	CRISPR spacer
ctttcaaaagaaaaaaattaattaacacgt	Protospacer
************** ****.*** *  .*

48. spacer 1.5|270129|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to MN794232 (Vibrio phage VH1_2019, complete genome) position: , mismatch: 7, identity: 0.767

tcgtctgtgcgccgaatagataaagaggtg	CRISPR spacer
tgaccggtgcgccgactagataaacaggta	Protospacer
* ..* ********* ******** ****.

49. spacer 1.5|270129|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to NC_005083 (Vibrio phage KVP40, complete genome) position: , mismatch: 7, identity: 0.767

tcgtctgtgcgccgaatagataaagaggtg	CRISPR spacer
tgcacggtgcgccgactagataaacaggta	Protospacer
*   * ********* ******** ****.

50. spacer 1.5|270129|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to JN849462 (Vibriophage phi-pp2, complete genome) position: , mismatch: 7, identity: 0.767

tcgtctgtgcgccgaatagataaagaggtg	CRISPR spacer
tgcacggtgcgccgactagataaacaggta	Protospacer
*   * ********* ******** ****.

51. spacer 1.5|270129|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to MT135024 (Vibrio phage V05, complete genome) position: , mismatch: 7, identity: 0.767

tcgtctgtgcgccgaatagataaagaggtg	CRISPR spacer
tgaccggtgcgccgactagataaacaggta	Protospacer
* ..* ********* ******** ****.

52. spacer 1.5|270129|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to AY283928 (Bacteriophage KVP40, complete genome) position: , mismatch: 7, identity: 0.767

tcgtctgtgcgccgaatagataaagaggtg	CRISPR spacer
tgcacggtgcgccgactagataaacaggta	Protospacer
*   * ********* ******** ****.

53. spacer 1.5|270129|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to MT135026 (Vibrio phage V09, complete genome) position: , mismatch: 7, identity: 0.767

tcgtctgtgcgccgaatagataaagaggtg	CRISPR spacer
tgaccggtgcgccgactagataaacaggta	Protospacer
* ..* ********* ******** ****.

54. spacer 1.5|270129|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to MT135025 (Vibrio phage V07, complete genome) position: , mismatch: 7, identity: 0.767

tcgtctgtgcgccgaatagataaagaggtg	CRISPR spacer
tgcacggtgcgccgactagataaacaggta	Protospacer
*   * ********* ******** ****.

55. spacer 1.3|269997|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to MN176217 (Escherichia phage vB_Eco4M-7, complete genome) position: , mismatch: 8, identity: 0.733

cagagtttcaagagctgcatgaattattcg	CRISPR spacer
ttaagcttcaagagctgtatgaattatatt	Protospacer
. .**.***********.********* .

56. spacer 1.3|269997|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to MH051335 (Escherichia phage vB_EcoM-Ro157lw, complete genome) position: , mismatch: 8, identity: 0.733

cagagtttcaagagctgcatgaattattcg	CRISPR spacer
ttaagcttcaagagctgtatgaattatatt	Protospacer
. .**.***********.********* .

57. spacer 1.3|269997|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to JX128258 (Escherichia phage ECML-117, complete genome) position: , mismatch: 8, identity: 0.733

cagagtttcaagagctgcatgaattattcg	CRISPR spacer
ttaagcttcaagagctgtatgaattatatt	Protospacer
. .**.***********.********* .

58. spacer 1.3|269997|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to NC_048194 (Escherichia phage vB_EcoM_WFH, complete genome) position: , mismatch: 8, identity: 0.733

cagagtttcaagagctgcatgaattattcg	CRISPR spacer
ttaagcttcaagagctgtatgaattatatt	Protospacer
. .**.***********.********* .

59. spacer 1.3|269997|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to MT446405 (UNVERIFIED: Escherichia virus TH32, complete genome) position: , mismatch: 8, identity: 0.733

cagagtttcaagagctgcatgaattattcg	CRISPR spacer
ttaagcttcaagagctgtatgaattatatt	Protospacer
. .**.***********.********* .

60. spacer 1.3|269997|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to MT446408 (UNVERIFIED: Escherichia virus TH35, complete genome) position: , mismatch: 8, identity: 0.733

cagagtttcaagagctgcatgaattattcg	CRISPR spacer
ttaagcttcaagagctgtatgaattatatt	Protospacer
. .**.***********.********* .

61. spacer 1.3|269997|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to NC_048195 (Escherichia phage vB_EcoM_WFC, complete genome) position: , mismatch: 8, identity: 0.733

cagagtttcaagagctgcatgaattattcg	CRISPR spacer
ttaagcttcaagagctgtatgaattatatt	Protospacer
. .**.***********.********* .

62. spacer 1.3|269997|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to NC_048073 (Escherichia phage FEC19, complete genome) position: , mismatch: 8, identity: 0.733

cagagtttcaagagctgcatgaattattcg	CRISPR spacer
ttaagcttcaagagctgtatgaattatatt	Protospacer
. .**.***********.********* .

63. spacer 1.3|269997|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to MT684590 (Streptomyces phage LilMartin, complete genome) position: , mismatch: 8, identity: 0.733

cagagtttcaagagctgcatgaattattcg	CRISPR spacer
gggagtttcaagagcttcatgatttcatga	Protospacer
 .************** ***** **  * .

64. spacer 1.3|269997|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to MT897905 (Streptomyces phage MulchMansion, complete genome) position: , mismatch: 8, identity: 0.733

cagagtttcaagagctgcatgaattattcg	CRISPR spacer
gggagtttcaagagcttcatgatttcatga	Protospacer
 .************** ***** **  * .

65. spacer 1.4|270063|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to MN693967 (Marine virus AFVG_250M992, complete genome) position: , mismatch: 8, identity: 0.733

ctttcaaaagaaaataattgattcaattga	CRISPR spacer
tttttaaaagaaaataatggattcacagct	Protospacer
.***.************* ******

66. spacer 1.4|270063|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP040783 (Bacillus thuringiensis strain HM-311 plasmid p1, complete sequence) position: , mismatch: 8, identity: 0.733

ctttcaaaagaaaataattgattcaattga	CRISPR spacer
ctttcaaaagaaaaaaattaattaccgcgt	Protospacer
************** ****.***    .*

67. spacer 1.4|270063|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP014283 (Bacillus thuringiensis strain Bt185 plasmid pBT1850636, complete sequence) position: , mismatch: 8, identity: 0.733

ctttcaaaagaaaataattgattcaattga	CRISPR spacer
ctttcaaaagaaaaaaattaattaccgcgt	Protospacer
************** ****.***    .*

68. spacer 1.4|270063|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP032614 (Bacillus thuringiensis strain QZL38 plasmid p.1, complete sequence) position: , mismatch: 8, identity: 0.733

ctttcaaaagaaaataattgattcaattga	CRISPR spacer
ctttcaaaagaaaaaaattaattaccgcgt	Protospacer
************** ****.***    .*

69. spacer 1.4|270063|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to CP046512 (Bacillus cereus strain JHU plasmid p1, complete sequence) position: , mismatch: 8, identity: 0.733

ctttcaaaagaaaataattgattcaattga	CRISPR spacer
ctttcaaaagaaaaaaattaattaccgcgt	Protospacer
************** ****.***    .*

70. spacer 1.4|270063|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP011154 (Bacillus cereus strain CMCC P0011 plasmid pRML05, complete sequence) position: , mismatch: 8, identity: 0.733

ctttcaaaagaaaataattgattcaattga	CRISPR spacer
ctttcaaaagaaaaaaattaattaccacgt	Protospacer
************** ****.***    .*

71. spacer 1.4|270063|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP011152 (Bacillus cereus strain CMCC P0021 plasmid pRML04, complete sequence) position: , mismatch: 8, identity: 0.733

ctttcaaaagaaaataattgattcaattga	CRISPR spacer
ctttcaaaagaaaaaaattaattaccacgt	Protospacer
************** ****.***    .*

72. spacer 1.4|270063|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP045607 (Bacillus cereus strain SB1 plasmid p1, complete sequence) position: , mismatch: 8, identity: 0.733

ctttcaaaagaaaataattgattcaattga	CRISPR spacer
ctttcaaaagaaaaaaattaattaccacgt	Protospacer
************** ****.***    .*

73. spacer 1.4|270063|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP050184 (Bacillus thuringiensis strain HER1410 plasmid pLUSID1, complete sequence) position: , mismatch: 8, identity: 0.733

ctttcaaaagaaaataattgattcaattga	CRISPR spacer
ctttcaaaagaaaaaaattaattaccgcgt	Protospacer
************** ****.***    .*

74. spacer 1.4|270063|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to NC_015583 (Novosphingobium sp. PP1Y plasmid Mpl, complete sequence) position: , mismatch: 8, identity: 0.733

ctttcaaaagaaaataattgattcaattga	CRISPR spacer
aacgaaaaagaaaataattgatttagttaa	Protospacer
  .  ******************.*.**.*

75. spacer 1.5|270129|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to KP671755 (Vibrio phage ValKK3, complete genome) position: , mismatch: 8, identity: 0.733

tcgtctgtgcgccgaatagataaagaggtg	CRISPR spacer
tgacaggtgcgccgactagataaacaggta	Protospacer
* ..  ********* ******** ****.

76. spacer 1.6|270195|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP049908 (Hymenobacter sp. HDW8 plasmid p_unnamed1, complete sequence) position: , mismatch: 8, identity: 0.733

cactatttggggagacgagcgtgacgagcc	CRISPR spacer
ccgactttggggcgacgagcctgacgagag	Protospacer
*    ******* ******* *******

77. spacer 1.4|270063|30|NZ_SRYT01000001|PILER-CR,CRISPRCasFinder,CRT matches to CP040041 (Acinetobacter baumannii strain VB958 plasmid unnamed1, complete sequence) position: , mismatch: 9, identity: 0.7

ctttcaaaagaaaataattgattcaattga	CRISPR spacer
taaagataagaaaataattgatttaattcc	Protospacer
.    * ****************.****

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

292571 : 301216

Synechococcus_phage(50.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_002381817.1\|292571_293144_-	phosphoribosylglycinamide formyltransferase	M4QRX9	Synechococcus_phage	5.4e-25	35.1
WP_002360140.1\|293140_294172_-	phosphoribosylformylglycinamidine cyclo-ligase	A0A0E3FGG1	Synechococcus_phage	8.2e-56	44.4
WP_002382407.1\|294173_295613_-	amidophosphoribosyltransferase	A0A0M3SGR2	Mollivirus	2.1e-49	33.0
WP_016633930.1\|295588_297808_-	phosphoribosylformylglycinamidine synthase subunit PurL	A6N228	Microbacterium_phage	1.3e-146	39.9
WP_002369271.1\|297804_298479_-	phosphoribosylformylglycinamidine synthase subunit PurQ	NA	NA	NA	NA
WP_002357271.1\|298479_298731_-	phosphoribosylformylglycinamidine synthase subunit PurS	NA	NA	NA	NA
WP_002369269.1\|298744_299458_-	phosphoribosylaminoimidazolesuccinocarboxamide synthase	A0A0E3ELR0	Synechococcus_phage	5.7e-48	43.0
WP_002379523.1\|299610_300735_-	5-(carboxyamino)imidazole ribonucleotide synthase	NA	NA	NA	NA
WP_002365766.1\|300727_301216_-	5-(carboxyamino)imidazole ribonucleotide mutase	A0A2P0VNU7	Tetraselmis_virus	6.4e-19	42.0

DBSCAN-SWA_2

482459 : 505993

Enterococcus_phage(54.55%)

capsid,terminase,portal,transposase,integrase

attL 485440:485454|attR 506692:506706

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_087548619.1\|482459_482942_-	fibronectin type III domain-containing protein	C9E2K1	Enterococcus_phage	1.9e-07	34.5
WP_157662384.1\|482956_483619_-	hypothetical protein	A0A2H4J709	uncultured_Caudovirales_phage	1.4e-08	27.2
WP_087548621.1\|484751_485339_-	DUF4355 domain-containing protein	NA	NA	NA	NA
WP_168354143.1\|485433_486135_-\|capsid	minor capsid protein	A0A090D822	Clostridium_phage	3.1e-22	30.6
485440:485454	attL	NA	NA	NA	NA
WP_135952840.1\|486205_487594_-\|portal	phage portal protein	A0A0M4S669	Bacillus_phage	2.1e-83	42.0
WP_087548624.1\|487606_489043_-\|terminase	phage terminase large subunit	A0A090EUA8	Clostridium_phage	6.3e-123	51.9
WP_087548625.1\|489020_489497_-\|transposase	transposase	D2IZL8	Enterococcus_phage	3.9e-61	82.1
WP_087548626.1\|489527_489758_-	hypothetical protein	NA	NA	NA	NA
WP_087548627.1\|490047_490263_-	hypothetical protein	NA	NA	NA	NA
WP_010815803.1\|491054_492101_-	hypothetical protein	NA	NA	NA	NA
WP_087548628.1\|492090_493908_-	SAM-dependent DNA methyltransferase	A0A2H4PQP4	Staphylococcus_phage	7.0e-10	26.6
WP_087548629.1\|494037_494454_-	autolysin	D2IZL7	Enterococcus_phage	7.8e-66	96.4
WP_168354144.1\|495219_495648_-	RusA family crossover junction endodeoxyribonuclease	D2IYU2	Enterococcus_phage	3.3e-67	90.8
WP_016632687.1\|495644_496598_-	phage replisome organizer protein	C9E2N2	Enterococcus_phage	6.8e-97	69.3
WP_010784721.1\|496601_497282_-	hypothetical protein	C9E2N1	Enterococcus_phage	3.6e-100	76.5
WP_087548631.1\|497278_498217_-	DUF1351 domain-containing protein	A0A0S2MYB3	Enterococcus_phage	2.9e-92	65.1
WP_002368205.1\|498201_498879_-	ERF family protein	A0A0S2MYH3	Enterococcus_phage	2.5e-114	92.9
WP_002368203.1\|498862_499117_-	hypothetical protein	A0A0S2MYD6	Enterococcus_phage	2.9e-23	74.4
WP_087548632.1\|499294_499633_-	hypothetical protein	NA	NA	NA	NA
WP_087548633.1\|499697_499880_-	hypothetical protein	D2IYT3	Enterococcus_phage	1.5e-18	78.9
WP_087548634.1\|499890_500076_-	hypothetical protein	D2IZW5	Enterococcus_phage	3.1e-22	95.0
WP_087548635.1\|500086_500800_-	phage regulatory protein	D2IYT0	Enterococcus_phage	1.2e-90	77.1
WP_002381719.1\|500838_501150_-	hypothetical protein	NA	NA	NA	NA
WP_087548636.1\|501165_501981_-	phage antirepressor KilAC domain-containing protein	R4IFK0	Staphylococcus_phage	8.7e-61	46.9
WP_087548637.1\|501992_502184_-	hypothetical protein	A0A1S5SA94	Streptococcus_phage	2.9e-07	43.5
WP_087548638.1\|502474_502843_+	helix-turn-helix transcriptional regulator	R4IH08	Staphylococcus_phage	2.0e-17	40.5
WP_157662385.1\|502832_503957_+	ImmA/IrrE family metallo-endopeptidase	A0A0F6N3H6	Staphylococcus_phage	6.9e-08	27.6
WP_087548640.1\|503972_504299_+	hypothetical protein	NA	NA	NA	NA
WP_087548641.1\|504460_504772_+	hypothetical protein	NA	NA	NA	NA
WP_002395797.1\|504844_505993_+\|integrase	site-specific integrase	A0A1C8E994	Bacillus_phage	2.9e-38	30.7
506692:506706	attR	NA	NA	NA	NA

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

5. NZ_SRYT01000002

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

108843 : 129253

Enterococcus_phage(37.5%)

tRNA,tail,holin

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_002361699.1\|108843_109440_-	N-acetyltransferase	A0A1P8CWJ6	Bacillus_phage	1.5e-22	37.1
WP_002363808.1\|109439_110390_-	riboflavin biosynthesis protein RibF	A0A1V0SD03	Indivirus	1.2e-05	28.7
WP_002379378.1\|110415_111336_-\|tRNA	tRNA pseudouridine(55) synthase TruB	NA	NA	NA	NA
WP_002382241.1\|111489_112614_-	N-acetylmuramoyl-L-alanine amidase	A0A139ZVY1	Enterococcus_phage	3.2e-77	44.3
WP_002357842.1\|112732_113128_-\|holin	phage holin family protein	F0PIJ6	Enterococcus_phage	2.8e-33	48.9
WP_002379376.1\|113151_114921_-	hypothetical protein	A0A097BYA8	Enterococcus_phage	1.5e-142	71.6
WP_002379375.1\|114953_116411_-	hypothetical protein	C9E2K6	Enterococcus_phage	5.5e-175	47.7
WP_002365553.1\|116423_117350_-\|tail	phage tail family protein	C9E2K5	Enterococcus_phage	9.0e-147	78.6
WP_087548577.1\|117351_120276_-	hypothetical protein	A0A1U9WR55	Streptococcus_virus	6.0e-19	30.2
WP_002382238.1\|120265_120559_-	hypothetical protein	NA	NA	NA	NA
WP_002357848.1\|120651_121011_-	hypothetical protein	A0A2P1JTV4	Anoxybacillus_phage	2.8e-11	36.0
WP_002365550.1\|121043_121556_-\|tail	phage major tail protein, TP901-1 family	W6LP72	Streptococcus_phage	8.8e-35	49.4
WP_002382237.1\|121568_121985_-	hypothetical protein	NA	NA	NA	NA
WP_002379371.1\|122206_122614_-	RinA family phage transcriptional activator	E9LUP5	Lactobacillus_phage	1.2e-15	37.7
WP_002360492.1\|122633_123026_-	hypothetical protein	NA	NA	NA	NA
WP_002379370.1\|123092_123935_-	ATP-binding protein	A0A0P0I3L9	Lactobacillus_phage	5.7e-31	31.0
WP_002382234.1\|123953_124709_-	DnaD domain protein	A0A0K0MX39	Streptococcus_phage	6.7e-31	56.0
WP_002360496.1\|125106_125589_+	helix-turn-helix domain-containing protein	R9QTP6	Staphylococcus_phage	2.6e-20	47.7
WP_002382232.1\|125623_126109_+	toxin	A0A097BY56	Enterococcus_phage	4.4e-60	70.5
WP_002382231.1\|126168_126399_-	hypothetical protein	NA	NA	NA	NA
WP_002360497.1\|126481_126832_-	30S ribosome-binding factor RbfA	NA	NA	NA	NA
WP_002357859.1\|126856_129253_-	translation initiation factor IF-2	A0A2H4UTS4	Bodo_saltans_virus	1.7e-19	24.1

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

6. NZ_SRYT01000006

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Crispr_ID: NZ_SRYT01000006_1

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

NZ_SRYT01000006_1

87240-87313

Orphan

Consensus_repeat	Method
GTTTTTCTGTTGCTCACCAACTAT	CRISPRCasFinder

1 spacers

The CRISPR arrays of NZ_SRYT01000006_1

>merge|NZ_SRYT01000006|1|87240-87313|CRISPRCasFinder
GTTTTTCTGTTGCTCACCAACTATTGGCTCTACGTCCAATTCTATATCGAGTTTTTCTGTTGCTCGCCAACTAT

>NZ_SRYT01000006|1|1|87240-87313|CRISPRCasFinder
GTTTTTCTGTTGCTCACCAACTAT	TGGCTCTACGTCCAATTCTATATCGA
GTTTTTCTGTTGCTCGCCAACTAT

Protein	Signature genes	Signature genes Name	Protein_function
NZ_SRYT01000006.1\|WP_002354864.1\|76939_77737_+\|TIGR00282-family-metallophosphoesterase	unknown	unknown	gnl\|CDD\|379099
NZ_SRYT01000006.1\|WP_033780130.1\|88299_91044_-\|glycoside-hydrolase-family-65-protein	unknown	unknown	gnl\|CDD\|224471
NZ_SRYT01000006.1\|WP_002385180.1\|85284_85818_-\|CPBP-family-intramembrane-metalloprotease	unknown	unknown	gnl\|CDD\|376806
NZ_SRYT01000006.1\|WP_002359020.1\|97362_98229_+\|helix-turn-helix-domain-containing-protein	unknown	unknown	gnl\|CDD\|224343
NZ_SRYT01000006.1\|WP_002354866.1\|77825_78197_+\|hypothetical-protein	unknown	unknown	gnl\|CDD\|226923
NZ_SRYT01000006.1\|WP_002378983.1\|94629_95898_+\|insulinase-family-protein	unknown	unknown	gnl\|CDD\|223685
NZ_SRYT01000006.1\|WP_087548688.1\|93214_93931_+\|WxL-domain-containing-protein	unknown	unknown	gnl\|CDD\|372694
NZ_SRYT01000006.1\|WP_002354877.1\|91305_92019_+\|trehalose-operon-repressor	unknown	unknown	gnl\|CDD\|274116
NZ_SRYT01000006.1\|WP_025187370.1\|92419_93118_+\|hypothetical-protein	unknown	unknown	gnl\|CDD\|372694
NZ_SRYT01000006.1\|WP_135952861.1\|82962_83517_+\|septum-formation-protein-Maf	unknown	unknown	gnl\|CDD\|238310
NZ_SRYT01000006.1\|WP_002367457.1\|87634_88285_-\|beta-phosphoglucomutase	unknown	unknown	gnl\|CDD\|213672
NZ_SRYT01000006.1\|WP_002381060.1\|84261_85233_+\|ribose-phosphate-diphosphokinase	unknown	unknown	gnl\|CDD\|234929
NZ_SRYT01000006.1\|WP_002354871.1\|83649_84087_+\|peptide-methionine-(R)-S-oxide-reductase-MsrB	unknown	unknown	gnl\|CDD\|234692
NZ_SRYT01000006.1\|WP_002378991.1\|80805_82938_+\|DNA-mismatch-repair-endonuclease-MutL	unknown	unknown	gnl\|CDD\|223400
NZ_SRYT01000006.1\|WP_002378992.1\|78197_80774_+\|DNA-mismatch-repair-protein-MutS	unknown	unknown	gnl\|CDD\|235444
NZ_SRYT01000006.1\|WP_002354863.1\|75223_76780_+\|ribonuclease-Y	unknown	unknown	gnl\|CDD\|237177
NZ_SRYT01000006.1\|WP_002359018.1\|93971_94421_-\|large-conductance-mechanosensitive-channel-protein-MscL	unknown	unknown	gnl\|CDD\|376605
NZ_SRYT01000006.1\|WP_002378981.1\|95890_97195_+\|insulinase-family-protein	unknown	unknown	gnl\|CDD\|223685
NZ_SRYT01000006.1\|WP_002359021.1\|98397_98610_+\|hypothetical-protein	unknown	unknown	unknown
NZ_SRYT01000006.1\|WP_002385181.1\|86579_87173_-\|hypothetical-protein	unknown	unknown	gnl\|CDD\|376232

Protein	Function_ID	Function_description	E-value
NZ_SRYT01000006.1\|WP_002354864.1\|76939_77737_+\|TIGR00282-family-metallophosphoesterase	gnl\|CDD\|379099	pfam13277, YmdB, YmdB-like protein. This family of putative phosphoesterases contains the B. subtilis protein YmdB.	2.68643e-155
NZ_SRYT01000006.1\|WP_033780130.1\|88299_91044_-\|glycoside-hydrolase-family-65-protein	gnl\|CDD\|224471	COG1554, ATH1, Trehalose and maltose hydrolases (possible phosphorylases) [Carbohydrate transport and metabolism].	1.50311e-171
NZ_SRYT01000006.1\|WP_002385180.1\|85284_85818_-\|CPBP-family-intramembrane-metalloprotease	gnl\|CDD\|376806	pfam02517, Abi, CAAX protease self-immunity. Members of this family are probably proteases (after a isoprenyl group is attached to the Cys residue in the C-terminal CAAX motif of a protein to attach it to the membrane, the AAX tripeptide being removed by one of the CAAX prenyl proteases). The family contains the CAAX prenyl protease. The proteins contain a highly conserved Glu-Glu motif at the amino end of the alignment. The alignment also contains two histidine residues that may be involved in zinc binding. While they are involved in membrane anchoring of proteins in eukaryotes, little is known about their function in prokaryotes. In some known bacteriocin loci, Abi genes have been found downstream of bacteriocin structural genes where they are probably involved in self-immunity. Investigation of the bacteriocin-like loci in the Gram positive bacteria locus from Lactobacillus sakei 23K confirmed that the bacteriocin-like genes (sak23Kalphabeta) exhibited antimicrobial activity when expressed in a heterologous host and that the associated Abi gene (sak23Ki) conferred immunity against the cognate bacteriocin. Interestingly, the immunity genes from three similar systems conferred a high degree of cross-immunity against each other's bacteriocins, suggesting the recognition of a common receptor. Site-directed mutagenesis demonstrated that the conserved motifs constituting the putative proteolytic active site of the Abi proteins are essential for the immunity function of Sak23Ki - thus a new concept in self-immunity.	1.58164e-07
NZ_SRYT01000006.1\|WP_002359020.1\|97362_98229_+\|helix-turn-helix-domain-containing-protein	gnl\|CDD\|224343	COG1426, COG1426, Predicted transcriptional regulator contains Xre-like HTH domain [Function unknown].	2.66641e-35
NZ_SRYT01000006.1\|WP_002354866.1\|77825_78197_+\|hypothetical-protein	gnl\|CDD\|226923	COG4550, COG4550, Predicted membrane protein [Function unknown].	8.72333e-15
NZ_SRYT01000006.1\|WP_002378983.1\|94629_95898_+\|insulinase-family-protein	gnl\|CDD\|223685	COG0612, PqqL, Predicted Zn-dependent peptidases [General function prediction only].	2.04695e-51
NZ_SRYT01000006.1\|WP_087548688.1\|93214_93931_+\|WxL-domain-containing-protein	gnl\|CDD\|372694	pfam13731, WxL, WxL domain surface cell wall-binding. The WxL motif appears in two or three copies in these bacterial proteins and confers a cell surface localization function. It seems likely that this region is the cell wall-binding domain of gram-positive bacteria, and may interact with the peptidoglycan.	8.64958e-25
NZ_SRYT01000006.1\|WP_002354877.1\|91305_92019_+\|trehalose-operon-repressor	gnl\|CDD\|274116	TIGR02404, Trehalose_operon_transcriptional_repressor, trehalose operon repressor, B. subtilis-type. This family consists of repressors of the GntR family typically associated with trehalose utilization operons. Trehalose is imported as trehalose-6-phosphate and then hydrolyzed by alpha,alpha-phosphotrehalase to glucose and glucose-6-P. This family includes repressors mostly from Gram-positive lineages and does not include the TreR from E. coli. [Regulatory functions, DNA interactions].	6.92052e-137
NZ_SRYT01000006.1\|WP_025187370.1\|92419_93118_+\|hypothetical-protein	gnl\|CDD\|372694	pfam13731, WxL, WxL domain surface cell wall-binding. The WxL motif appears in two or three copies in these bacterial proteins and confers a cell surface localization function. It seems likely that this region is the cell wall-binding domain of gram-positive bacteria, and may interact with the peptidoglycan.	4.98763e-09
NZ_SRYT01000006.1\|WP_135952861.1\|82962_83517_+\|septum-formation-protein-Maf	gnl\|CDD\|238310	cd00555, Maf, Nucleotide binding protein Maf. Maf has been implicated in inhibition of septum formation in eukaryotes, bacteria and archaea, but homologs in B.subtilis and S.cerevisiae are nonessential for cell division. Maf has been predicted to be a nucleotide- or nucleic acid-binding protein with structural similarity to the hypoxanthine/xanthine NTP pyrophosphatase Ham1 from Methanococcus jannaschii, RNase H from Escherichia coli, and some other nucleotide or RNA-binding proteins.	1.03845e-85
NZ_SRYT01000006.1\|WP_002367457.1\|87634_88285_-\|beta-phosphoglucomutase	gnl\|CDD\|213672	TIGR01990, bPGM, beta-phosphoglucomutase. This model represents the beta-phosphoglucomutase enzyme which catalyzes the interconverison of beta-D-glucose-1-phosphate and beta-D-glucose-6-phosphate. The 6-phosphate is capable of non-enzymatic anomerization (alpha <-> beta) while the 1-phosphate is not. A separate enzyme is responsible for the isomerization of the alpha anomers. Beta-D-glucose-1-phosphate results from the phosphorylysis of maltose (2.4.1.8), trehalose (2.4.1.64) or trehalose-6-phosphate (2.4.1.216). Alternatively, these reactions can be run in the synthetic direction to create the disaccharides. All sequenced genomes which contain a member of this family also appear to contain at least one putative maltose or trehalose phosphorylase. Three species, Lactococcus, Enterococcus and Neisseria appear to contain a pair of paralogous beta-PGM's. Beta-phosphoglucomutase is a member of the haloacid dehalogenase superfamily of hydrolase enzymes. These enzymes are characterized by a series of three catalytic motifs positioned within an alpha-beta (Rossman) fold. beta-PGM contains an inserted alpha helical domain in between the first and second conserved motifs and thus is a member of subfamily IA of the superfamily. The third catalytic motif comes in three variants, the third of which, containing a conserved DD or ED, is the only one found here as well as in several other related enzymes (TIGR01509). The enzyme from L. lactis has been extensively characterized including a remarkable crystal structure which traps the pentacoordinate transition state. [Energy metabolism, Biosynthesis and degradation of polysaccharides].	1.52041e-106
NZ_SRYT01000006.1\|WP_002381060.1\|84261_85233_+\|ribose-phosphate-diphosphokinase	gnl\|CDD\|234929	PRK01259, PRK01259, ribose-phosphate diphosphokinase.	0
NZ_SRYT01000006.1\|WP_002354871.1\|83649_84087_+\|peptide-methionine-(R)-S-oxide-reductase-MsrB	gnl\|CDD\|234692	PRK00222, PRK00222, peptide-methionine (R)-S-oxide reductase MsrB.	2.48715e-93
NZ_SRYT01000006.1\|WP_002378991.1\|80805_82938_+\|DNA-mismatch-repair-endonuclease-MutL	gnl\|CDD\|223400	COG0323, MutL, DNA mismatch repair enzyme (predicted ATPase) [DNA replication, recombination, and repair].	0
NZ_SRYT01000006.1\|WP_002378992.1\|78197_80774_+\|DNA-mismatch-repair-protein-MutS	gnl\|CDD\|235444	PRK05399, PRK05399, DNA mismatch repair protein MutS; Provisional.	0
NZ_SRYT01000006.1\|WP_002354863.1\|75223_76780_+\|ribonuclease-Y	gnl\|CDD\|237177	PRK12704, PRK12704, phosphodiesterase; Provisional.	0
NZ_SRYT01000006.1\|WP_002359018.1\|93971_94421_-\|large-conductance-mechanosensitive-channel-protein-MscL	gnl\|CDD\|376605	pfam01741, MscL, Large-conductance mechanosensitive channel, MscL.	2.66655e-49
NZ_SRYT01000006.1\|WP_002378981.1\|95890_97195_+\|insulinase-family-protein	gnl\|CDD\|223685	COG0612, PqqL, Predicted Zn-dependent peptidases [General function prediction only].	1.62226e-84
NZ_SRYT01000006.1\|WP_002385181.1\|86579_87173_-\|hypothetical-protein	gnl\|CDD\|376232	pfam18813, PBECR4, phage-Barnase-EndoU-ColicinE5/D-RelE like nuclease4. A predicted endoRNase of the Barnase-EndoU-ColicinE5/D-RelE like nuclease fold found in polyvalent proteins of phages and conjugative elements. The predicted active site contains a conserved histidine residue.	2.0486e-23

>NZ_SRYT01000006.1|WP_002385181.1|86579_87173_-|hypothetical-protein
MIEKKDFDTIRKAFLIYDTNFNNHMFTYTYQHRKTKKLLEMNVQFEAGNFMHLCGVDYYQIDKQGSCKFRFKATQFYRALKNNKVSLRGIKPKDDGTTGQKLQVIPLLEMLISPGVRICDGGKFYNLQYEKAIRSGKMIVALTCKENNKKYVPQSLLSLINQPRKSQSKSLTESHEVIKISKSELNSTSVIEVYDKF
>NZ_SRYT01000006.1|WP_002385180.1|85284_85818_-|CPBP-family-intramembrane-metalloprotease
MSLIAIKNNEKALIIFDSVLSLIIIIVLSVFYKKIVKKKKQAIFKSLLLAIGMFLFSIILNLLFSRIPTPENQHYINEVRNVAPVLMALHTRLFSPIIEELLTRGIFMNLFFIKNTSRSVLCKIFFSGLFFGLSHGLLPSISMLFYCGMGWVLASTYYFCNNNLTYPLAIHLLLNNL
>NZ_SRYT01000006.1|WP_002381060.1|84261_85233_+|ribose-phosphate-diphosphokinase
MSKHYFDPRLKIFSLNSNRPLAEKIADAVGVELGKCSVTQFSDGEIQVNIEESIRGAHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYARQDRKARAREPITAKLVANMIEKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIADYFIEHGIQGDDVVVVSPDHGGVTRARKLAEFLKAPIAIIDKRRPKANVAEVMNIIGHVEGKTCVLIDDMIDTAGTISLAANALKEAGAKDVYASCTHPVLSGPALQRIEDSAIERLVVTDSIYLSDDRKIAKIDEVSVGELIGDAIKRIHENKPVSPLFETKNK
>NZ_SRYT01000006.1|WP_002354871.1|83649_84087_+|peptide-methionine-(R)-S-oxide-reductase-MsrB
MTKPTEEELKQTLTDLQYAVTQENATERPFSGEYDDFYQDGIYVDIVSGEPLFSSLDKYDAGCGWPSFTKPIEKRGVKEKADFSHGMHRVEVRSQEADSHLGHVFTDGPLQEGGLRYCINAAALRFVPVADLEKEGYGEYLSLFK
>NZ_SRYT01000006.1|WP_135952861.1|82962_83517_+|septum-formation-protein-Maf
MQIILASQSPRRQELLKRVVPTFTIAPADIDETVGKDGLPAEYVAQMAAQKAAAIAEQSPEALVIGCDTIVALAGEILGKPTSREDGYRMLRLLSGKTHDVYTSVTLKQGEKERSATVHSTVTFYPLTDTEIHAYLDTAEYADKAGAYGIQGQGALLIEAIAGDYYAIMGLPIVKVARLLKEFN
>NZ_SRYT01000006.1|WP_002378991.1|80805_82938_+|DNA-mismatch-repair-endonuclease-MutL
MGKIQELSEQLANQIAAGEVVERPASVVKELVENALDAGSTQIDIFIEEAGLKTIQIIDNGEGIAKEDVLNAFKRHATSKIHTRDDLFRIRSLGFRGEALPSIASVSEMIVETATAEEEEGSYVILKGGKVEENRPAALRKGTKMTVSNLFYNTPARLKYVKTIQTELANIGDIVNRLALSHPKVAFRLVHDGHKMMSTTGNGDLKQTIAGIYGISTAKKMLKIEGKDLDFTLTGYVSLPEVTRASRNYLSTIINGRYIKNFALNKAIVAGYGSKLMVGRFPIAVLEIEMDPLLVDVNVHPTKQEVRLSKEKDLMALIEQAIQEVLSQEQLIPNAADNLRFKKKLPPEPKAEQMEIPLSTEQPAVKQRPGSLSYNPATGRFYAEHATDETPASSAPEAVNQTERAVDPMDDPFAPPVPESTSVAEETSAYETSSAAADSANHWETASNEQQVIAEETKITEVPDSEPSLRSSEAADEVFQEEMKLHPEFDANSAASQRELKQALNKLEEERPTERFPELEYFGQMHGTYLFAQSKDGLFIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPIVIDYPNSDALKIKEQKETLAEVGIHLEDFGQNSFIVRAHPTWYPAGEEESIVREMIDMLLTTGSVSVKKFREATAIMMSCKRSIKANHYLNEQQARVLLKDLALCENPFNCPHGRPVLIHFTNSDMERMFKRIQDPH
>NZ_SRYT01000006.1|WP_002378992.1|78197_80774_+|DNA-mismatch-repair-protein-MutS
MPQKTKNTPMMEQYLSIKAQYKDAFLFYRLGDFYELFYEDAINAAQILELTLTSRNRNADDPIPMCGVPYHAAQGYIDTLIEQGYKVAICEQVEDPKTTKGMVKREVVQLVTPGTVMNSKGLEAKDNNYLTAVLTDGNQFGFAYVDLSTGELKTAVLADEEGVLNEASALQTKEMVLGSGIPESLKENLSLRLNIIFSTQETVEENAEFSFLTNELINPLEIEITGKLLSYLSVTQKRSLSHIQKAVEYQPDHFLKMDHYSKFNLELSQSIRTGQKKGTLLWLLDETKTAMGGRLLKQWLDRPLIQERQIKARQEMVQSLLNAYFERLDLQAALTNVYDLERLAGRVAFGSVNGRDLIQLRTSLEQVPTIRQLIVGINQGEWDDLLVDLNPVEDLVALIATAINEEAPLQITEGKVIKDGYNDQLDEYRDAMRNGKQWLAELEAKERQETGIKNLKIGYNRVFGYFIEITKSNLANLEEGKYERKQTLANAERFITPELKELERLILEAEEKSVELEYQLFLAVREQVKTNIDRLQTLAKTISAVDVLQSFATISERYQYVRPTLRSNTKNLAIVEGRHPVVEKVLGHQEYIPNSIRMNPETDILLITGPNMSGKSTYMRQLALTVVMAQIGCFVPAESAEMPIFDQIFTRIGASDDLIAGQSTFMVEMMEANQALRHATPNSLILFDELGRGTATYDGMALAQAIIEYIHREVQAKTLFSTHYHELTVLDETLKGLKNIHVGAVEKDGEVVFLHKMMEGPADKSYGIHVAKIAGLPSPLLERAATILSALEAEETTIPISVHHEEVSEVHEETEQLSLFKEVSTEELSVIDTLKKMNLLEMTPLDALNMLHQLQKRI
>NZ_SRYT01000006.1|WP_002354866.1|77825_78197_+|hypothetical-protein
MEPLNLDAQSNKELEKLLHLIGQDEVIQRYQAIEEKVKKNKKLTELVEEIKAAQKDAVQFAHYGKPTAEKEAIQRADAKTKEFDEHPLVVAYREQLIEANDLVQHVTALIQYRVNEELEKEGN
>NZ_SRYT01000006.1|WP_002354864.1|76939_77737_+|TIGR00282-family-metallophosphoesterase
MRILFIGDVVGSLGREALATYVPKLKKKYRPQVTIANGENAASGRGITGKIYKKFLQDGVDVVTMGNHTWDNKDIFEFIDDAKKMVRPANFPEDVPGQGMVFVKVNQLELAVINLQARTFMVPLEDPFKKAAELVAIARKRTPLIFVDFHGETTSEKQAMSWFLDGQVSAVVGTHTHVQTNDARILPKGTAFLSDVGMTGPYDGILGMKREPIIEKFMTALPKRFEVVETGRTILSACILEIDDQTGQAKMIEPIQISEDRPFQE
>NZ_SRYT01000006.1|WP_002354863.1|75223_76780_+|ribonuclease-Y
MVLNILLAIVGLIVGLGLGFVIAKSRHDKAINGAKISASSILENARKESETLKKEALLEAKEENQKYRSEIESELKESRLELKSQENRLIQREQTLDRKDDSLEKREGSLEEKEEKLGARQQLIDEREKEVENLINQQHQELERIASLSKEEAKSIIMKSTEEELNHELTLMVKESEQRAKEESDRKAKNLLSLAIQRCAADSVSETTVSVVTLPNDEMKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGRIHPARIEEMVEKSRKEMDERIREYGEQAAFEVGAHTLHPDLIKILGRLRFRTSYGQNVLNHSIEVAKLAGVLAAELGEDVQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYKENSVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLENLENISNSFEGVDSSFAVQAGREVRVMVKPEEISDLESVRLVRDIRKKIEDDLDYPGHIKVTVIRETRAVDYAK
>NZ_SRYT01000006.1|WP_002367457.1|87634_88285_-|beta-phosphoglucomutase
MTIGFIFDLDGVITDTAKFHYQAWKALADSLGIPIDETFNETLKGISRMDSLDRILAHGHRENAFTPAEKEALAQQKNDHYVQLLEHLTTEDVLPGVVPLLQQAQARHIPCAVASASKNAPLILEKLGVRAYFATIVDPDSLSKGKPDPEIFLAAADSIGVLPQNAIGFEDAQSGIDGLKAAGIYAVGLSASQPLIGADMQVSEMTELSVDALLNR
>NZ_SRYT01000006.1|WP_033780130.1|88299_91044_-|glycoside-hydrolase-family-65-protein
MSIQVYEEAPNMTILELTFATNQLTTTFFNHKLEPLHQKTFPFEALTIEETVPELCQLILAEATPILGGIVADFPADFFSPEETLALVPQAVIQAFAEQLNTPLLTAAEQAAAPNLLEAFEEKRQYLAWHLDYYGYVPGKNEYAVESLLTVGNGFLGLRGTTPEMTISDDHYPATYIAGLYNTAASEVAGQVVENEDFVNAPDNQHIALKIGDATDWLTISPDTLQQLHRQLNLKTGLFVAEMILKDADNQQIKLTTKKIANMAQPNDYHLQYTFEPLNFSAPITLKTVTDGSVYNYNVARYRNLTAKHFQVTALSAQENKTVIEVCTNQSNLSVRETALITGDFFEKEAIMIQEEAEKIAQVVTVMAHQGTRYTLEKQVFVQASHAEQSWQVPFTPKDSFAAAAQESARAWQTLWQQANITVTGDLMSQKLLRIHSYHLLASASPFSNQAQALDVSITARGLHGEAYRGHIFWDEIFILPFYIQHYPDTARQLLLYRYHRLEKAKENAAASQYRGAMYPWQSGRDGRETTQKLHLNPLNGHWGEDHSILQRHVSLAIAYNAWLYWHSTQDHEFMKQYGGEMLLEIAQFWNSAATLDDATGRFFIDKVMGPDEFHEGYPDQAESGLKNNAYTNLMVVWLFEELTNILALFSEEEQAQLFAKTQTSSADLARMQQIQNSLEIEVNSDGIIAQYEGYFGLKEIDWTAMKEKYGNIYRMDRILKAEGESPDDYKVAKQADTLMLFYNLDKTRVDQILEDLGYQLPADYLEKNLLYYLKRTSHGSTLSRIVHAQLAEMAQFHELSWQLYQEALYSDYRDIQGGTTAEGIHTGVMAATIHVTLATYAGVDTRQNELSICPNLPEHWQALTFQFIHQGVTYQFSLTQTSVTITADRDTQLLVQGALIPLTAERPKEVHYQ
>NZ_SRYT01000006.1|WP_002354877.1|91305_92019_+|trehalose-operon-repressor
MNKFHDIFLELEQQIVQGDYQPGDLLPSENQLVETYNVSRETIRKALNLLINAGYIQKKQGKGSIVLNFKKFNFPISGVTSYKELQQTQHIESHTHVVSLEEIPVERNLAQLTGWTEGAPVWRLVRQREIEGEVDILDIDYLIKEIVPELPKHRAEDSIFDYLENDLGLAISYAQKEITVEPLTEMDKQLIGEVEGNHVVVVRSIMNLEDTRCFGYTESRHRLNKFKFVEFARRRKL
>NZ_SRYT01000006.1|WP_025187370.1|92419_93118_+|hypothetical-protein
MKKKMMASLLVGSAVVGASLAPLSAQATTTGNTPVQVEFGGGVLPDHEDNTGENVAPDPTKPNTDFDLLVIPRNFNFSPVKIGEDISAIKLASSLTKTIYVGDVRGTKEGWHVTGEITEMKNAEDSLSGEINFGLTPYYAQYDSTLQGYTATSTLNGVNIVDEPTAPIFSGTNMKIGGGAATLITASVGNGQGTWASGFNNVSLNVTTPMQQIKAGAYTGNITWNLVAGPSI
>NZ_SRYT01000006.1|WP_087548688.1|93214_93931_+|WxL-domain-containing-protein
MKKKMMASLLVGSAVVGASLAPLSAQATTTGNTPVTATFEGGTLPDGNGDANTVDPDPNGSNSNFDLLFIPREFNFGKLSISDDLTKPILNQRDPEAINGHTEMIGVGDVRGGKEGWHLTAQSNDLKLGTESLQGNIKVALITQLDLEYEATNNSYVALTTAVSAPEVRPQLAAPQNWTLDLDGEAQLMANAAAGQGQGLWQFSLFNTALNITTPANNIKAGAYTGSITWNLVAGPSV
>NZ_SRYT01000006.1|WP_002359018.1|93971_94421_-|large-conductance-mechanosensitive-channel-protein-MscL
MIKEFKEFIMRGSVLDLAVGVVIGSAFTAIVTQVVEGLITPLISLIFVLTTGKKSADDALGALVYKVEGVEFNIGSVISALITFLITAFVLFLIVKAANKMKNRGKKEEAAEEEVVPTSEDYLKEIRDLLAAQTPPAETVKTDSTVTEK
>NZ_SRYT01000006.1|WP_002378983.1|94629_95898_+|insulinase-family-protein
MSVQLVKGVNLHVIPTEKYKTVRLLVRFNTRLNHETITKRTLLSSLMETNSLNYPNQVKLSERLAELYGASFGIGVSKKGNQHWFNVSMNIVNDRYLQDSQVLAEAVDFLKEIIFAPNIQAGQFEAETFQREKENLKAYLESIVEDKQTYASLALQSVYFNQSEDQKIPSFGTVAALAEETAASLAAYYQKMLAEDQVDIFVLGDVNEAELVPLFKQLPFTPREEGKAAIFYNQPIRNVIEERTEREVLAQSKLNLAYNTDIYYGDSYYFALQVFNGIFGGFPHSKLFMNVREKEHLAYYASSSIDTFRGFMTVQTGIDGKNRNQVLRLISTELENIRLGKISELEIEQTKAMLKNQYILALDNAGAWLEKEYLNQLMPQTMLTAEEWIARINAVTISEIQEVAKRLELQAIFFLEGETEND
>NZ_SRYT01000006.1|WP_002378981.1|95890_97195_+|insulinase-family-protein
MIKKDYAQIKETLYTETLANGLKVYLLPKNDFQKTYGLFTTDYGSIDNTFVPIGQEEMIEVPDGIAHFLEHKMFEKEDGDVFQKFGQQGASANAFTSFTKTSYLFSTTDQVTQNLETLLDFVQSPYFTKETVEKEKGIIGQEIQMYLDDPNWRLFFGILGNLYPKHPLHIDIAGTVASIAEITAEDLYTCYNTFYHPSNMTLFVVGKMDPEALMAFIRENQAAKEFPAAQPIRRHFPEETAADIVKESAIEMAVTRAKVLVGLKGLDEVPTTGRDLMKYQVTVNLLLQLLFGNTSQNYLALYDEGLLDDSFSYDFNLDRSFHFADIGGDSEQPEVLAERIEAILLSATTSPELTEENLSLLKKKMIGKYFQSLNSLEYIANQFSQSLFGETTLFDKIEVIESIQLADVRQVAETFVREEGLSRFYMYPKGETEQ
>NZ_SRYT01000006.1|WP_002359020.1|97362_98229_+|helix-turn-helix-domain-containing-protein
MASVNIGETLKEARLQKNISIDELQQMTKIQKRYLEIIEQNDFESLPGTFYVRAFIRQYASAVGLDGNQLVDIYDGKEPAIVEEPKPVYEELEGSRKQLHDEEKQSSWLLRNLPAIAFSLIGLAIAVVVLYIMWQDQKAEPIIQTPATSASVEKPKESSQAPESSTQSSTPPSSSSEPEKKTAVAVVSDANNQVTATVKDANSPINITFKGTEGSCWIGVMVDGNYTYQHTLQAGETQTTALPANAANATLILGASNNVAIQVDGADLTLEKTPVLIRKDVTLGITYR
>NZ_SRYT01000006.1|WP_002359021.1|98397_98610_+|hypothetical-protein
MLKIVNKKFNLITIFSIALLVMGFLIVGLQLYFYFIYGSKFLDSITLIGMINLGLGYYQLFLKKGKNISE

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Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region	Region Position	Protein_number	Hit_taxonomy	Key_proteins	Att_site	Prophage annotation

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

7. NZ_SRYT01000004

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CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

78253 : 86167

Planktothrix_phage(33.33%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_002364714.1\|78253_79306_+	ABC transporter ATP-binding protein	G9BWD6	Planktothrix_phage	2.0e-17	30.1
WP_002355795.1\|79305_80262_+	ATP-binding cassette domain-containing protein	G9BWD6	Planktothrix_phage	8.5e-23	33.9
WP_002355796.1\|80302_81373_+	helix-turn-helix domain-containing protein	A0A288TXV8	Enterococcus_phage	7.6e-89	49.0
WP_002355797.1\|81772_82294_+	translation initiation factor IF-3	A0A2L0UZ54	Agrobacterium_phage	3.0e-14	33.3
WP_002355798.1\|82407_82608_+	50S ribosomal protein L35	NA	NA	NA	NA
WP_002355799.1\|82668_83028_+	50S ribosomal protein L20	NA	NA	NA	NA
WP_002385048.1\|83073_83454_-	hypothetical protein	NA	NA	NA	NA
WP_002361363.1\|83521_83881_+	DUF1304 domain-containing protein	NA	NA	NA	NA
WP_025187394.1\|83948_84443_+	GNAT family N-acetyltransferase	NA	NA	NA	NA
WP_002379186.1\|84460_84976_+	GrpB family protein	A0A2K5B2B6	Erysipelothrix_phage	1.0e-38	48.8
WP_002380126.1\|84991_86167_-	SulP family inorganic anion transporter	A0A2H4J153	uncultured_Caudovirales_phage	3.3e-85	47.7

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage