CRISPRimmunity

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Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Target MGE spacer number	Prophage number
CP024102	Bacillus cytotoxicus strain CH_25 plasmid pCh25_53, complete sequence	0 crisprs	NA	0	1
CP024103	Bacillus cytotoxicus strain CH_25 plasmid pCh25_67, complete sequence	0 crisprs	TnsE_C	0	1
CP024101	Bacillus cytotoxicus strain CH_25 chromosome, complete genome	3 crisprs	cas3,csa3,WYL,DinG,cas6,cas8b2,cas7,cas5,cas2,cas1,cas9,cas4,DEDDh,cas14j	4	10

Results visualization

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CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

15638 : 22255

Bacillus_phage(33.33%)

The bacterium proteins that are colored denote the protein is present at specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin' and 'tRNA')

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AWC34977.1\|15638_16757_-	hypothetical protein	A0A2H4J8G3	uncultured_Caudovirales_phage	3.6e-97	51.3
AWC34978.1\|17143_17449_+	hypothetical protein	NA	NA	NA	NA
AWC34979.1\|17513_18014_-	hypothetical protein	NA	NA	NA	NA
AWC34980.1\|18405_18828_-	hypothetical protein	NA	NA	NA	NA
AWC34981.1\|18870_19440_-	resolvase	A0A0A8WIK3	Clostridium_phage	4.8e-42	49.7
AWC34982.1\|19660_19945_+	CRISPR-associated protein Cas2	A0A0M3LS55	Mannheimia_phage	3.5e-09	38.9
AWC34983.1\|20018_20369_-	YolD-like family protein	A0A1Q1PW34	Staphylococcus_phage	3.0e-10	39.7
AWC34984.1\|20365_21631_-	DNA repair protein	O64031	Bacillus_phage	1.7e-103	47.4
AWC34985.1\|21820_22255_-	sporulation protein	F8WPS9	Bacillus_phage	4.1e-33	50.7

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

2. CP024103

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CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

10107 : 17361

Bacillus_phage(33.33%)

integrase

attL 7031:7046|attR 23239:23254

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
7031:7046	attL	NA	NA	NA	NA
AWC35029.1\|10107_10680_+	hypothetical protein	A0A0K2SUB9	Clostridium_phage	3.3e-14	44.4
AWC35030.1\|11156_11342_-	DNA-binding protein	A6M9A5	Geobacillus_virus	6.8e-14	64.9
AWC35031.1\|11380_11719_-	hypothetical protein	NA	NA	NA	NA
AWC35032.1\|12162_13119_-\|integrase	integrase	A0A1B1P793	Bacillus_phage	1.2e-37	34.3
AWC35033.1\|13235_13850_-	DNA recombinase	A0A1V0E035	Clostridioides_phage	7.1e-15	32.5
AWC35034.1\|15052_15259_+	hypothetical protein	NA	NA	NA	NA
AWC35035.1\|15488_16088_+	hypothetical protein	A0A1Z1LZN4	Bacillus_phage	5.1e-10	35.6
AWC35036.1\|16641_17361_+	DNA (cytosine-5-)-methyltransferase	A0A1I9KKE7	Lactobacillus_phage	7.4e-64	52.0
23239:23254	attR	NA	NA	NA	NA

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

3. CP024101

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CRISPR-Cas detection and classification

Crispr_ID: CP024101_1

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

CP024101_1

1577543-1577671

Orphan

Consensus_repeat	Method
AACTAGGGTGGTACCGCGATGTTTTAATCGTCCCTACG	CRISPRCasFinder

1 spacers

The CRISPR arrays of CP024101_1

>merge|CP024101|1|1577543-1577671|CRISPRCasFinder
AACTAGGGTGGTACCGCGATGTTTTAATCGTCCCTACGTATTTGCGTAGGGGCGTTTTTTATTGAAGGTCCTACTGTCGTTGTGTCTTCATAACTAGGGTGGTACCGCGATATTTTAATCGTCCCTACG

>CP024101|1|1|1577543-1577671|CRISPRCasFinder
AACTAGGGTGGTACCGCGATGTTTTAATCGTCCCTACG	TATTTGCGTAGGGGCGTTTTTTATTGAAGGTCCTACTGTCGTTGTGTCTTCAT
AACTAGGGTGGTACCGCGATATTTTAATCGTCCCTACG

Protein	Signature genes	Signature genes Name	Protein_function
CP024101.1\|AWC32396.1\|1566857_1567670_+\|UDP-galactose-lipid-carrier-transferase	unknown	unknown	gnl\|CDD\|274736
CP024101.1\|AWC32408.1\|1583305_1583404_+\|hypothetical-protein	unknown	unknown	gnl\|CDD\|378838
CP024101.1\|AWC32411.1\|1587707_1590248_+\|thiamine-biosynthesis-protein-ThiF	unknown	unknown	gnl\|CDD\|366360
CP024101.1\|AWC32394.1\|1564453_1565830_+\|N-acetylmuramoyl-L-alanine-amidase	unknown	unknown	gnl\|CDD\|226656
CP024101.1\|AWC34867.1\|1563697_1563886_-\|hypothetical-protein	unknown	unknown	unknown
CP024101.1\|AWC32406.1\|1580452_1580989_-\|peptide-methionine-(S)-S-oxide-reductase	unknown	unknown	gnl\|CDD\|376578
CP024101.1\|AWC32400.1\|1573288_1574497_+\|MFS-transporter	unknown	unknown	gnl\|CDD\|341031
CP024101.1\|AWC32395.1\|1565885_1566350_-\|hypothetical-protein	unknown	unknown	unknown
CP024101.1\|AWC32403.1\|1577774_1579112_+\|sodium-dependent-transporter	unknown	unknown	gnl\|CDD\|271367
CP024101.1\|AWC32397.1\|1568168_1569428_+\|GTPase-HflX	unknown	unknown	gnl\|CDD\|274455
CP024101.1\|AWC32401.1\|1574512_1575430_+\|cysteine-synthase-A	unknown	unknown	gnl\|CDD\|273465
CP024101.1\|AWC32402.1\|1575499_1577134_-\|polysaccharide-biosynthesis-protein	unknown	unknown	gnl\|CDD\|240529
CP024101.1\|AWC32398.1\|1569759_1571328_+\|Vga-family-ABC-F-type-ribosomal-protection-protein	unknown	unknown	gnl\|CDD\|223562
CP024101.1\|AWC32412.1\|1590406_1591621_+\|MFS-transporter	unknown	unknown	gnl\|CDD\|340949
CP024101.1\|AWC32399.1\|1571430_1573071_-\|peptide-ABC-transporter-substrate-binding-protein	unknown	unknown	gnl\|CDD\|173869
CP024101.1\|AWC32407.1\|1581907_1582807_+\|branched-chain-amino-acid-aminotransferase	unknown	unknown	gnl\|CDD\|183549
CP024101.1\|AWC32410.1\|1585445_1586708_+\|threonine-dehydratase	unknown	unknown	gnl\|CDD\|236318
CP024101.1\|AWC32409.1\|1583738_1585412_+\|dihydroxy-acid-dehydratase	unknown	unknown	gnl\|CDD\|234861
CP024101.1\|AWC32404.1\|1579383_1579650_+\|hypothetical-protein	unknown	unknown	gnl\|CDD\|337191
CP024101.1\|AWC32405.1\|1579789_1580428_+\|elongation-factor-G-binding-protein	unknown	unknown	gnl\|CDD\|319974

Protein	Function_ID	Function_description	E-value
CP024101.1\|AWC32396.1\|1566857_1567670_+\|UDP-galactose-lipid-carrier-transferase	gnl\|CDD\|274736	TIGR03708, poly_P_AMP_trns, polyphosphate:AMP phosphotransferase. Members of this protein family contain a domain duplication. The characterized member from Acinetobacter johnsonii is polyphosphate:AMP phosphotransferase (PAP), which can transfer the terminal phosphate from poly(P) to AMP, yielding ADP. In the opposite direction, this enzyme can synthesize poly(P). Each domain of this protein family is homologous to polyphosphate kinase, an enzyme that can run in the forward direction to extend a polyphosphate chain with a new terminal phosphate from ATP, or in reverse to make ATP (or GTP) from ADP (or GDP). [Central intermediary metabolism, Phosphorus compounds].	1.35596e-112
CP024101.1\|AWC32408.1\|1583305_1583404_+\|hypothetical-protein	gnl\|CDD\|378838	pfam12323, HTH_OrfB_IS605, Helix-turn-helix domain. This is the N terminal helix-turn-helix domain of Transposase_2 pfam01385.	0.000629588
CP024101.1\|AWC32411.1\|1587707_1590248_+\|thiamine-biosynthesis-protein-ThiF	gnl\|CDD\|366360	pfam00899, ThiF, ThiF family. This domain is found in ubiquitin activating E1 family and members of the bacterial ThiF/MoeB/HesA family. It is repeated in Ubiquitin-activating enzyme E1.	1.77744e-21
CP024101.1\|AWC32394.1\|1564453_1565830_+\|N-acetylmuramoyl-L-alanine-amidase	gnl\|CDD\|226656	COG4193, LytD, Beta- N-acetylglucosaminidase [Carbohydrate transport and metabolism].	1.27967e-24
CP024101.1\|AWC32406.1\|1580452_1580989_-\|peptide-methionine-(S)-S-oxide-reductase	gnl\|CDD\|376578	pfam01625, PMSR, Peptide methionine sulfoxide reductase. This enzyme repairs damaged proteins. Methionine sulfoxide in proteins is reduced to methionine.	4.36886e-93
CP024101.1\|AWC32400.1\|1573288_1574497_+\|MFS-transporter	gnl\|CDD\|341031	cd17478, MFS_FsR, Fosmidomycin resistance protein of the Major Facilitator Superfamily of transporters. Fosmidomycin resistance protein (FsR) confers resistance against fosmidomycin. It shows sequence similarity with the bacterial drug-export proteins that mediate resistance to tetracycline and chloramphenicol. This FsR family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.	1.4256e-102
CP024101.1\|AWC32403.1\|1577774_1579112_+\|sodium-dependent-transporter	gnl\|CDD\|271367	cd10336, SLC6sbd_Tyt1-Like, solute carrier 6 subfamily, Fusobacterium nucleatum Tyt1-like; solute-binding domain. SLC6 proteins (also called the sodium- and chloride-dependent neurotransmitter transporter family or Na+/Cl--dependent transporter family) include neurotransmitter transporters (NTTs): these are sodium- and chloride-dependent plasma membrane transporters for the monoamine neurotransmitters serotonin (5-hydroxytryptamine), dopamine, and norepinephrine, and the amino acid neurotransmitters GABA and glycine. These NTTs are widely expressed in the mammalian brain, involved in regulating neurotransmitter signaling and homeostasis, and the target of a range of therapeutic drugs for the treatment of psychiatric diseases. Bacterial members of the SLC6 family include the LeuT amino acid transporter. An arrangement of 12 transmembrane (TM) helices appears to be as a common topological motif for eukaryotic and some prokaryotic and archaeal NTTs. However, this subfamily which contains the majority of bacterial members and some archaeal members, appears to contain only 11 TMs; for example the functional Fusobacterium nucleatum tyrosine transporter Tyt1.	0
CP024101.1\|AWC32397.1\|1568168_1569428_+\|GTPase-HflX	gnl\|CDD\|274455	TIGR03156, GTP_HflX, GTP-binding protein HflX. This protein family is one of a number of homologous small, well-conserved GTP-binding proteins with pleiotropic effects. Bacterial members are designated HflX, following the naming convention in Escherichia coli where HflX is encoded immediately downstream of the RNA chaperone Hfq, and immediately upstream of HflKC, a membrane-associated protease pair with an important housekeeping function. Over large numbers of other bacterial genomes, the pairing with hfq is more significant than with hflK and hlfC. The gene from Homo sapiens in this family has been named PGPL (pseudoautosomal GTP-binding protein-like). [Unknown function, General].	0
CP024101.1\|AWC32401.1\|1574512_1575430_+\|cysteine-synthase-A	gnl\|CDD\|273465	TIGR01139, Cysteine_synthase, cysteine synthase A. This model distinguishes cysteine synthase A (CysK) from cysteine synthase B (CysM). CysM differs in having a broader specificity that also allows the use of thiosulfate to produce cysteine thiosulfonate. [Amino acid biosynthesis, Serine family].	1.17556e-161
CP024101.1\|AWC32402.1\|1575499_1577134_-\|polysaccharide-biosynthesis-protein	gnl\|CDD\|240529	cd13124, MATE_SpoVB_like, Stage V sporulation protein B, also known as Stage III sporulation protein F, and related proteins. The integral membrane protein SpoVB has been implicated in the biosynthesis of the peptidoglycan component of the spore cortex in Bacillus subtilis. This model represents a subfamily of the multidrug and toxic compound extrusion (MATE)-like proteins. Proteins from the MATE family are involved in exporting metabolites across the cell membrane and are often responsible for multidrug resistance (MDR).	2.49597e-129
CP024101.1\|AWC32398.1\|1569759_1571328_+\|Vga-family-ABC-F-type-ribosomal-protection-protein	gnl\|CDD\|223562	COG0488, Uup, ATPase components of ABC transporters with duplicated ATPase domains [General function prediction only].	3.36502e-113
CP024101.1\|AWC32412.1\|1590406_1591621_+\|MFS-transporter	gnl\|CDD\|340949	cd17391, MFS_MdtG_MDR_like, Multidrug resistance protein MdtG and similar multidrug resistance (MDR) transporters of the Major Facilitator Superfamily. This subfamily is composed of Escherichia coli multidrug resistance protein MdtG, Streptococcus pneumoniae multidrug resistance efflux pump PmrA, and similar multidrug resistance (MDR) transporters from bacteria. MDR transporters are drug/H+ antiporters (DHA) that mediate the efflux of a variety of drugs and toxic compounds, and confer resistance to these compounds. MdtG confers resistance to fosfomycin and deoxycholate. PmrA serves as an efflux pump for various substrates and is associated with fluoroquinolone resistance. MdtG-like MDR transporters belong to the bacterial MdtG-like and eukaryotic solute carrier 18 (SLC18) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.	2.55865e-172
CP024101.1\|AWC32399.1\|1571430_1573071_-\|peptide-ABC-transporter-substrate-binding-protein	gnl\|CDD\|173869	cd08504, PBP2_OppA, The substrate-binding component of an ABC-type oligopetide import system contains the type 2 periplasmic binding fold. This family represents the periplasmic substrate-binding component of an ATP-binding cassette (ABC)-type oligopeptide transport system comprised of 5 subunits. The transport system OppABCDEF contains two homologous integral membrane proteins OppB and OppF that form the translocation pore; two homologous nucleotide-binding domains OppD and OppF that drive the transport process through binding and hydrolysis of ATP; and the substrate-binding protein or receptor OppA that determines the substrate specificity of the transport system. The dipeptide (DppA) and oligopeptide (OppA) binding proteins differ in several ways. The DppA binds dipeptides and some tripeptides and is involved in chemotaxis toward dipeptides, whereas the OppA binds peptides of a wide range of lengths (2-35 amino acid residues) and plays a role in recycling of cell wall peptides, which precludes any involvement in chemotaxis. Most of other periplasmic binding proteins are comprised of only two globular subdomains corresponding to domains I and III of the dipeptide/oligopeptide binding proteins. The structural topology of these domains is most similar to that of the type 2 periplasmic binding proteins (PBP2), which are responsible for the uptake of a variety of substrates such as phosphate, sulfate, polysaccharides, lysine/arginine/ornithine, and histidine. The PBP2 bind their ligand in the cleft between these domains in a manner resembling a Venus flytrap. After binding their specific ligand with high affinity, they can interact with a cognate membrane transport complex comprised of two integral membrane domains and two cytoplasmically located ATPase domains. This interaction triggers the ligand translocation across the cytoplasmic membrane energized by ATP hydrolysis. Besides transport proteins, the PBP2 superfamily includes the ligand-binding domains from ionotropic glutamate receptors, LysR-type transcriptional regulators, and unorthodox sensor proteins involved in signal transduction.	0
CP024101.1\|AWC32407.1\|1581907_1582807_+\|branched-chain-amino-acid-aminotransferase	gnl\|CDD\|183549	PRK12479, PRK12479, branched-chain-amino-acid transaminase.	0
CP024101.1\|AWC32410.1\|1585445_1586708_+\|threonine-dehydratase	gnl\|CDD\|236318	PRK08639, PRK08639, threonine dehydratase; Validated.	0
CP024101.1\|AWC32409.1\|1583738_1585412_+\|dihydroxy-acid-dehydratase	gnl\|CDD\|234861	PRK00911, PRK00911, dihydroxy-acid dehydratase; Provisional.	0
CP024101.1\|AWC32404.1\|1579383_1579650_+\|hypothetical-protein	gnl\|CDD\|337191	pfam08765, Mor, Mor transcription activator family. Mor (Middle operon regulator) is a sequence specific DNA binding protein. It mediates transcription activation through its interactions with the C-terminal domains of the alpha and sigma subunits of bacterial RNA polymerase. The N terminal region of Mor is the dimerization region, and the C terminal contains a helix-turn-helix motif which binds DNA.	0.00419013
CP024101.1\|AWC32405.1\|1579789_1580428_+\|elongation-factor-G-binding-protein	gnl\|CDD\|319974	cd16342, FusC_FusB, Fusidic acid resistance protein (FusC/FusB). The fusidic acid resistance protein FusC (FusB) mediates resistance to the antibiotic fusidic acid. Its C-terminal domain, which contains a zinc-binding site, interacts with EF-G with high affinity, promoting the dissociation of stalled ribosome#EF-G#GDP complexes that form in the presence of fusidic acid, thus allowing the ribosomes to resume translation.	2.03194e-116

>CP024101.1|AWC32402.1|1575499_1577134_-|polysaccharide-biosynthesis-protein
MSTSKVLKGTALLSGATMISRILGFIYFFPFQLLVGTQGVALYGYAYSWYGILLSFSTAGIPIAVSKFVAKYNALGDYSTSKKLYNSSVKLMLLMGFSGFFILFIGAPYISQFIIRSKAPDPKFIADVTLTMRALSFALIIVPAMSVTRGYFQGFQHMKPSAVSQVVEQIARVVFILAGSFVVTKLLGGSVASSVAVATFGAAVGAIASVSILIMYWKKYNHLKPPKGELKSRASTLPLKSIYMELLRYAIPIVFVGIAIPLYTLVDQYTVADALRALGEPLTTANAVFAYIANYAQKLIMIPASLATGFSLTIIPAITKSFTSGKQEELQEQITKIFQVLLFFTIPAAFGLASIAYDTFRMVYVSHDIAVGGSQYLISYAPSAVLSAIFTVSAAILQGIDYQRKTMIAFSVGILVKLIVNTPLLYVLGGHGAVLGTILGYLVSDAIMLYCIVKFAKFDIKETIKTVCLIIIYSGAMSAIVMALKAMIGWFIPGQSYIESLLIVIICGVVGGFVYLLFVLTSGLASRILGNRIQRLPILGKFIK
>CP024101.1|AWC32401.1|1574512_1575430_+|cysteine-synthase-A
MKLCKNVTELIGDTPVVRLSKFVPKDAADVYVKLEMFNPSRSVKDRAAYHLIHVAEEQGFIQPGDTIIEPTSGNTGIGLAMNAAAKGYKAILIMPDNMSKERMNLLKAYGAEVILTPAEQRMPGAIAKAIELQKQIPNSFIPQQFENPANPNIHRYTTALEIYEQMEGKLDAFVATAGTGGTITGTGETLKEKLPNLHIVVVEPKGSPVLSGGVPGPHKLVGTSPGFIPKNLNTEIYNEIIQIADEEALSTMRNLAKQEGLLVGPSSGASVYAAIMIAKRLGAGKKVLCIAPDTGERYLSMGLFE
>CP024101.1|AWC32400.1|1573288_1574497_+|MFS-transporter
MQAISKENVVTTPTIYRILFAISFGHFLNDSMQAVVPALFPILEKTMNLSYMQVGWIAFALNMTSSVMQPVFGMYSDKKPSPFLLPLGMFSSMLGMIGLAFAPNFIIVIISVLFIGLGSAVFHPEGARVAYMASGTKRGLAQAIYQVGGNTGNSLAPIFTAVIFVPLGQKGSLGFTAFAAVGILLLMYVSKWYKNELVSGAVKSKKRAALEAENAIVSAHIKFVIVLLIFLTFVRSWYGAGIGNFYQFYLIEHYGLSIKNAQYFVFAFMISGVLGTFFGGPLADRFGKKNIIVFSMLGSAPLALLLPHVSLLWVVPLFLCIGFISSSSFSVIVVYAQELVPGKVGMVSGLIVGLAFGLGALGAVVLGKLADIYSLQFIMVLCSCLPLIGLTSWLLPSDKKSV
>CP024101.1|AWC32399.1|1571430_1573071_-|peptide-ABC-transporter-substrate-binding-protein
MKKKTKRVAALSLTLSLVLTACGQKQETTTNKDEKKVATKQILNIVETGEISTLDSSIATDGFSYAALNNVMEGLYMPGPGNKPVPGAAESYTLSEDGKTYIFKLRHSNWSNGDPVTAHDFEYAWKRAINPETASEYAHIMFDIKNAEKVNKKALPLDSFGVKALDDKTLEVQLEHPVPYFLGLMGFATFYPQNQKVVEAQGTNYGLEAANAVYNGPFVLNEWKHDTSYKMTKNPNYWDHKNVKLQEINVNIVKDTSAAVNLFESKQVDRITINSEFVDKYQNDPSLKKMARPSVTFFRLNQKNPLLANKNARKAISLSFDKKGITTTILNNGSIPANAFIPKGFVKGPDNKDFRSTSNVKVETNIKEAKKQWETAKKETNLQNVSLSIISTDESNDKKISEYLKGELEKNLPGLKITLNPLPVKAFLEREGNGDFEISLSTWGPDYPDPTTFLNMFVTDGPFNKMNYSNKQYDELMEKASSSLTTDLPARWKAFQDAEKILVEDDAAIAPIFQAGLIYLERPSVKGVVVRPFAGIYSYKWAYITE
>CP024101.1|AWC32398.1|1569759_1571328_+|Vga-family-ABC-F-type-ribosomal-protection-protein
MLLLEANRIKYYIQDRLLLDIDQLQIHQNARIGLVGRNGSGKTTLFQILAKEIVPEEGSIVQRVRCELLPQLKQTDTTKSGGEVTQEYIQKAVLKDPELLLADEPTTNLDTGHIEWLEKKLGNWKGAFVIISHDRAFLDALCTTIWELDEGKIKVYTGNYSEYVKQKEIERQQEQLAYEKYEKKKKQLEEAVKLKEKKAERATKTPKKEGKLEPKITKPYFAKKQKKLQKTATAMITRLEKLGKVEKVKELPPLKMDLPNAETFKNRIILRVNDRIGKVGKRVLWKKASFHIRGGDKLAIIGANGSGKTTLVKQIMNQEYGIRFSPAVKVGYFSQNLNILDPAKSIIENVQSSSKQNETLIRTVLARMHFFREDVYKLVEVLSGGERVKVALTKLFLSDINTLILDEPTNFLDMTAVEALESLLKEYEGSVIFVSHDRRFIENIATRILTIHNQELGLFEGTYQKYKRTQVQQAQERKKDTRLLLETKLSEVLSRLSIEPSEKLEREFEKLLQEKRELDASE
>CP024101.1|AWC32397.1|1568168_1569428_+|GTPase-HflX
MEEFVQRAILVGVNLGHESDFQYSMEELANLAEACDVEVVGQVTQNLQRVNPSHYIGTGKIGEVAAYVKEVDANIVIFNDELSPSQIRNLEKDLDCKVIDRTILILDIFAQRAKTKEAQLQVEVAHLQYMMPRLVGLRESLGRQSGGVGTKNKGVGEKKLELDRRKIEEQISVLNKELEALVVQRKTQRKQRKKNEIPVVALVGYTNAGKSTTMNAMLEIFNGTIEKQVFEKDMLFATLETSVRNIELPDNKSFLLTDTVGFVSKLPHHLVKAFRSTLEEVAEADLLIHVVDYSNPNYEQLIEITNQTLKQIGVENIPTIYAYNKSDMMDVEIPKTKKDRVYLSAKKQIGMEELVEMIRSHIYKEYTKCEMLIPYDQGQIVSYFNEYAHVICTSYENEGTKLSLECKISDFEKYKRFAI
>CP024101.1|AWC32396.1|1566857_1567670_+|UDP-galactose-lipid-carrier-transferase
MKKEWLANVDLTKKIDSKSKYNKKLKKYQRHLLALQQILKEEKVAVIMVMEGWDAAGKGGAIKRVTEHLDPRGFQVKPIAAPAPHEKRYHYLQRFWREIPRYGQIAIFDRSWYGRVLVERVEGFATQEEWARAYDEINDFEKVLTDDHYIIGKFFYHMSKDEQLKRFKERENNPLKRWKITDEDWRNREKWDEYVEAMEEMFEKTNQPNAKWHIIASNNKLYARVKTLKVMISLIEDYFLEHGIELPSYYYEIKAEQQEEFEIVQDVSMK
>CP024101.1|AWC32395.1|1565885_1566350_-|hypothetical-protein
MKRKIFFLLTLLCIVLPIQVSAFTFQELPVASKSKQWYIEIDKSYNSNKQAIQPKQGVYDTYSLFVKNIGKDVSNVSIEIVGNDPSAKTTFKPTVPHTNVSKSHTSFDYMVFPLYFTSHTFKVIMTWEEKEFTYNGHPESKSKKMKQTFIFTEE
>CP024101.1|AWC32394.1|1564453_1565830_+|N-acetylmuramoyl-L-alanine-amidase
MKKVISNVLAMTAALQVIMVPTTSFAAEKGFSDVPKSHWAYEAINDLANRNIIAGYDNGKFGLGDSVTREQVAALIYRALKPEAKAEYKNPYHDVSASTTMFPNEILALTEMGIFTGDENKNFRPKDSLTRAEMAMILQRAYHLKVKANHTFHDVDPNSWAKDAISALQSNGMAEGDGTGAFYPSKTVTREEYAQFLFNAEQSYLNLDLTLASNVTAEEIDNFLKKSRSDSPLIGHGQDFIAAQNEHGVNALYLAAHAILESGYGRSEIAYRKHNLFGLRAYDRDPFYHAKYLPTYRDSISYNANYVRERYLEKGAIYYNGPTLVGMNVKYASDPEWAGKIAGLMERIKPFDRNDYKNANRLPKNPHTLDVEALGNEIPYKDLGNREIAVQASGKYYKVPYPYDLKIKSIPDITQNEMGTLTNGSKVTVHREDPNGWVEFSMKDKQEKYWTLKSNLKM
>CP024101.1|AWC34867.1|1563697_1563886_-|hypothetical-protein
MPKSINFSAFIDWGCFRIETELSYIQTCIYEELSTKKRYHVYTDIPFLAIFLLAPFSVSLFL
>CP024101.1|AWC32403.1|1577774_1579112_+|sodium-dependent-transporter
MNSPQWTSKLGFVLAAAGSAIGLGAIWKFPYMAGIGGGGAFFLIFIGFTLLIGLPLLLAEFVIGRSTQKEAVDAYRQIAPKTLWPWLGKLGVVTCFILLSFYSVVGGWILLYLWNAITGRLWEGNGAYEATFGEIISNPYLAVGSQLLFILMTIFIVSKGVQNGVEKVNKYFMPALFVLFFILIIRALTLDGAGEGVRFFLQPDFSNVTSEIILYAMGQSFFSLSVGVAVMVTYSSYLPKEESLPRSAFSIVGLTLMITLLAGLAIFPVVFAFGMEPSQGPGLLFIVLPAIFSKMAFGKVFFVIFLLLFFFATVTSAISMLEISVASLTAKGNGRREKFAIIVGLLIFVVGIPSALSYGVLSDFKVFGKTVFDLADFAVSNVLMPLGVLLVSIFVPLKMKKDVLMRELGVSKHKGYTLFILWLFLLRYIAPVAIIIVFLNVIGVI
>CP024101.1|AWC32404.1|1579383_1579650_+|hypothetical-protein
MGYVKATAVLPESLIAEIQKYVQGETIYIPKQETEHYKWGTRSGGRKQIDMRNRKIKEAFENGIAIYQLAEEYFLSVETIKKIVYSKS
>CP024101.1|AWC32405.1|1579789_1580428_+|elongation-factor-G-binding-protein
MEAFIRSDQYNFIKSQTHILVHGHATANDRGVITALKSLAIEKVLHAFENLNSEQKELIETILAVENREDAEAFLLKLNPYVIPFQEVTPQALKKLFPKVKKLKLPEMEEVNMKEMSYLGWTDKGTGKRFIIAKKNNKLVGVQGTFQSINKKGICALCHGHEEVGMFLVEIKGDVPGTFVKKGNYICQDSIACNQNMTSLDKLHDFIERLKK
>CP024101.1|AWC32406.1|1580452_1580989_-|peptide-methionine-(S)-S-oxide-reductase
MSKKTFELATFAGGCFWCMVKPFDELPGIQKVVSGYTGGHISNPTYEQVKSGTSGHLEVVQMTFDPSIFPYQKLLDLYWSQIDPTDDGGQFFDRGPSYRTAIFYHNETQKELAEKSKQLLATSGIFNEPIVTEIRPAAPFYEAEEYHQHFYKKNPKKYAQERKESGREDFIKTNWQKK
>CP024101.1|AWC32407.1|1581907_1582807_+|branched-chain-amino-acid-aminotransferase
MKKQYIYMNGELVEKEKAVVSVYDHGFLYGDGVFEGIRSYGGNVFCLKEHVKRLYESAKSILLTIPMTVEEMEKAVVQTLQKNGYADAYIRLIVSRGKGDLGLDPRSCEKPSVIIIAEQLKLFPQEFYDNGLSVVSVASRRNMPDALDPRIKSMNYLNNVLVKIEAAQAGALEALMLNQQGYVCEGSGDNVFVVKDGKVVTPPSYLGALEGVTRNSVIELCDKLGIPCEERPFTRHDVYVADEVFLTGTAAELIPVVKVDAREIGDGKPGEVTKQLTEAFKRLTRERGVRVPGLAESLA
>CP024101.1|AWC32408.1|1583305_1583404_+|hypothetical-protein
MLVNKVYKFRIYPNKGQEILKQVVDVLQVNTS
>CP024101.1|AWC32409.1|1583738_1585412_+|dihydroxy-acid-dehydratase
MRSDMIKKGFDKAPHRSLLKATGLKDEDFHKPFVAICNSFIEIIPGHKHLNEFGRLVKEAVRAAGMVPFEFNTIGVDDGIAMGHIGMRYSLPSREIIADSVETVVNAHWFDGMICIPNCDKITPGMMMAAMRINIPTVFVSGGPMAAGKTSKGEVVDLSSVFEGVGAYQSGKISEEELKDIEDHGCPSCGSCSGMFTANSMNCLCEVLGLALPGNGSILATDSRREQLIQRAAESLKILMERDIKPRDIVTEEAIDDAFALDMAMGGSTNTVLHTLAIAQEAGLDYDMNRIDAVSRKVPHLCKVSPASNWHMEDIDRAGGMSAILKELSRKEGVLHFDRITVTGKTLRENIADAEIQDKEVIHSLENPHSQEGGLRILKGNLAKDGAVIKSGATEVKRFEGPCVIFNSQDEALAGIMLGKVKKGDVVVIRYEGPRGGPGMPEMLAPTSAIAGMGLGADVALLTDGRFSGASRGISVGHISPEAAAGGEIALLQQGDIVCIDVEERLLEVKVSDEELAKRRKEWKRPEPKVKTGWLGRYAQMVTSANTGAVLKMQNFD
>CP024101.1|AWC32410.1|1585445_1586708_+|threonine-dehydratase
MVHKVKERVKIEDILMAHNCLKDIVNKTPLQRDAVLSEKYKCEVYVKREDLQVIRSFKIRGAYNLIQSLSKEKLQNGVVCASAGNHAQGVAYTCNLLEIPANIFMPTTTPKQKVSQVKFFGGDVADIMLVGDTFDSSFQEAQRYCEENGMTFVHPFDDPHVIAGQGTVAVEILHDTEKTIDYVFTAIGGGGLASGIGTYVKGVSPNTKVIGVEPKGAASMKEAFLQNENVTLEMIDSFVDGAAVKKVGKLTFETCKDVLDDIVLVPEGKICTTILELYKKNAIVAEPAGALSIAALDFYKDEIKGKTVVCVLSGGNNDIDRMQEMKERSLIYEGIKHYFIIQFPQRSGALREFLDKGLGPEDDITRFEYIKKHNKENGPALVGVELKHKEDYEPLIARFKENHIQFLELNKNPVLFDLLT
>CP024101.1|AWC32411.1|1587707_1590248_+|thiamine-biosynthesis-protein-ThiF
MRLKTSLNFRGYPNRNEIVYYDGEERVIEVNEFEKLWSLLKILESPKEDVHTKIQEWKNNNDIEDEELHQILQFINENGMLYEKRCDKMDEEQLYNIRNFHYFSTHDSTIYADAIVQRIKKVKAVVIGAGTIGATLCMTLSKLGVGEIIVIDFDTVQLKNIRAQTIFQKEDTNKKKIHVIQEKLKKMDPYVKVQVYDMKIETIHDLLRVDLHDVHYIFGCFDESSLQLQKDIMNYCDKEKIQYYLMGYHNDFVKVFHVSNRNDGERLLEESFQNYHTEYVIRENRGTIIQSLAVSLIISRILFEDITKSSCTVPSGYHFDFITFQTSHNRQSISREPFVQSLQRIMPFDQEQLNRKIEFLFNIIDKKEKVTILPKVIEMDILSMHQVFDILFHIGQIASLQLEDHYNKFIELMNEIDKTEDPEHNEYEQYLQFIRSMKINYEDEVYTIFEIFEMIRNTKDYEEKKKMQSGIYEVLKQNGDTLLSFFVNSKKKYLALEIPNYYMEVFGVKEETLHILENELQKKFHTLLTKSLSMMFSNSFHEIGVDFLSYNEEEHSMITLDEAKHFIVTSLEKDGKHHFVHYIERMFEENFIQVYNNVEVNKTYYFPSMKESRIVFNYHNDMDSVFVLCHELGHAYFNQSYGHTFFDDSTQLVNEMMAYYFEIICIQSMLGNEEIKIEMKQEIARQYIKRIHQTVLSTYGVHLLEKSLVKHIEEHGTISLLDFLKIRDEYNQHSFFKGIKFKNEKYFYLNPLLKSSFMLEFGEHLLPPMAYLLAVSLYNDRSETSIPKDIRMQEAIYNGVYCTEEFLSYVAKDVPHDERMKQAIHTLLELFCKLESFTMKDEVYSN
>CP024101.1|AWC32412.1|1590406_1591621_+|MFS-transporter
MASWKRNLMICWLGCFTTAAGMSLVIPFLSFYIEKLGVTGTSNIAQWSGIAFGVTFLMGAIVSPLWGKLGDTHGRKLMLIRASLGMAIIMTLMGFVTNVYQLVALRFLMGAVSGFLSTAMTFIAAETPKEHSGWAISTISTGGVSGSLLGPMLGGYLSELIGMRYVFFVTGAFLFLSFIIVFFFLKEENRSVQPKKAQPKKVWTMVPAKHFIMSLFVVTFMIQLANMSIQPIITLYVKHLAGAHTAHIETIAGAVMSATGLAVILAAPRLGRLSDHIGPQKTLIVALFVAGIVFIPQAFVTSAWQLLILRFLLGIAQAGLLPSVQTLLKKHTPNHITGRIFGYNQSFQFLGNMIGPVLGGQVAAHAGFQYVFFSTAALLFIACIWVYFHNRHAEVTGKEGLKVS

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Crispr_ID: CP024101_2

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

CP024101_2

2037734-2038165

TypeII

Consensus_repeat	Method
GCCATAATTCCTCTTTAAATCTTGGTATGATATGAT	PILER-CR
GCCATAATTCCTCTTTAAATCTTGGTATGATATGAT	CRISPRCasFinder
GCCATAATTCCTCTTTAAATCTTGGTATGATATGAT	CRT

6 spacers

cas2,cas1,cas9

The CRISPR arrays of CP024101_2

>merge|CP024101|2|2037734-2038165|PILER-CR,CRISPRCasFinder,CRT
GCCATAATTCCTCTTTAAATCTTGGTATGATATGATCCACCTTTGAAACGCGTCTGGATCAACGAGGCCATAATTCCTCTTTAAATCTTGGTATGATATGATAAAGACCCTACACATAATGAATGGAAATATGCCATAATTCCTCTTTAAATCTTGGTATGATATGATTACAAAAACAAATGAAATTATCGATAGCCGGCCATAATTCCTCTTTAAATCTTGGTATGATATGATTAAACCACTTATGGAATCTGTAACAATAACGCCATAATTCCTCTTTAAATCTTGGTATGATATGATTAGCCAAATTTCAATGTCATCGGAAATAATGCCATAATTCCTCTTTAAATCTTGGTATGATATGATAAAGACGCGATTGATTTTGCTGAGTATAAAGCCATAATTCCTCTTTAAATCTTGGTATGATATGAT

>CP024101|2|1|2037734-2038165|PILER-CR
GCCATAATTCCTCTTTAAATCTTGGTATGATATGAT	CCACCTTTGAAACGCGTCTGGATCAACGAG
GCCATAATTCCTCTTTAAATCTTGGTATGATATGAT	AAAGACCCTACACATAATGAATGGAAATAT
GCCATAATTCCTCTTTAAATCTTGGTATGATATGAT	TACAAAAACAAATGAAATTATCGATAGCCG
GCCATAATTCCTCTTTAAATCTTGGTATGATATGAT	TAAACCACTTATGGAATCTGTAACAATAAC
GCCATAATTCCTCTTTAAATCTTGGTATGATATGAT	TAGCCAAATTTCAATGTCATCGGAAATAAT
GCCATAATTCCTCTTTAAATCTTGGTATGATATGAT	AAAGACGCGATTGATTTTGCTGAGTATAAA
GCCATAATTCCTCTTTAAATCTTGGTATGATATGAT

>CP024101|2|2|2037734-2038165|CRISPRCasFinder
GCCATAATTCCTCTTTAAATCTTGGTATGATATGAT	CCACCTTTGAAACGCGTCTGGATCAACGAG
GCCATAATTCCTCTTTAAATCTTGGTATGATATGAT	AAAGACCCTACACATAATGAATGGAAATAT
GCCATAATTCCTCTTTAAATCTTGGTATGATATGAT	TACAAAAACAAATGAAATTATCGATAGCCG
GCCATAATTCCTCTTTAAATCTTGGTATGATATGAT	TAAACCACTTATGGAATCTGTAACAATAAC
GCCATAATTCCTCTTTAAATCTTGGTATGATATGAT	TAGCCAAATTTCAATGTCATCGGAAATAAT
GCCATAATTCCTCTTTAAATCTTGGTATGATATGAT	AAAGACGCGATTGATTTTGCTGAGTATAAA
GCCATAATTCCTCTTTAAATCTTGGTATGATATGAT

>CP024101|2|1|2037734-2038165|CRT
GCCATAATTCCTCTTTAAATCTTGGTATGATATGAT	CCACCTTTGAAACGCGTCTGGATCAACGAG
GCCATAATTCCTCTTTAAATCTTGGTATGATATGAT	AAAGACCCTACACATAATGAATGGAAATAT
GCCATAATTCCTCTTTAAATCTTGGTATGATATGAT	TACAAAAACAAATGAAATTATCGATAGCCG
GCCATAATTCCTCTTTAAATCTTGGTATGATATGAT	TAAACCACTTATGGAATCTGTAACAATAAC
GCCATAATTCCTCTTTAAATCTTGGTATGATATGAT	TAGCCAAATTTCAATGTCATCGGAAATAAT
GCCATAATTCCTCTTTAAATCTTGGTATGATATGAT	AAAGACGCGATTGATTTTGCTGAGTATAAA
GCCATAATTCCTCTTTAAATCTTGGTATGATATGAT

Protein	Signature genes	Signature genes Name	Protein_function
CP024101.1\|AWC32796.1\|2032840_2033845_-\|hypothetical-protein	unknown	unknown	gnl\|CDD\|378848
CP024101.1\|AWC32800.1\|2038319_2038628_-\|CRISPR-associated-endonuclease-Cas2	cas2	COG3512_cas2_CAS-II-A:CAS-II-B:CAS-II-C	gnl\|CDD\|226043
CP024101.1\|AWC32790.1\|2027199_2028054_-\|Cof-type-HAD-IIB-family-hydrolase	unknown	unknown	gnl\|CDD\|319818
CP024101.1\|AWC32795.1\|2032275_2032686_-\|hypothetical-protein	unknown	unknown	unknown
CP024101.1\|AWC32792.1\|2029103_2029259_-\|DUF1540-domain-containing-protein	unknown	unknown	gnl\|CDD\|377869
CP024101.1\|AWC34885.1\|2048146_2048794_-\|Na+/myo-inositol-cotransporter	unknown	unknown	gnl\|CDD\|236699
CP024101.1\|AWC32805.1\|2044544_2046890_-\|translation-initiation-inhibitor	unknown	unknown	gnl\|CDD\|372436
CP024101.1\|AWC32801.1\|2038632_2039535_-\|type-II-CRISPR-associated-endonuclease-Cas1	cas1	cd09720_cas1_CAS-II-A:CAS-II-B:CAS-II-C	gnl\|CDD\|274694
CP024101.1\|AWC32797.1\|2033857_2034391_-\|hypothetical-protein	unknown	unknown	unknown
CP024101.1\|AWC32807.1\|2049332_2049833_-\|N-acetyltransferase	unknown	unknown	gnl\|CDD\|273701
CP024101.1\|AWC32791.1\|2028255_2028834_-\|cysteine-dioxygenase	unknown	unknown	gnl\|CDD\|380416
CP024101.1\|AWC32798.1\|2034466_2034769_-\|hypothetical-protein	unknown	unknown	unknown
CP024101.1\|AWC32799.1\|2037465_2037630_-\|hypothetical-protein	unknown	unknown	gnl\|CDD\|275209
CP024101.1\|AWC32804.1\|2044087_2044396_-\|hypothetical-protein	unknown	unknown	unknown
CP024101.1\|AWC32802.1\|2039524_2042758_-\|type-II-CRISPR-RNA-guided-endonuclease-Cas9	cas9	COG3513_cas9_CAS-II-A:CAS-II-B:CAS-II-C	gnl\|CDD\|226044
CP024101.1\|AWC34886.1\|2049080_2049248_-\|hypothetical-protein	unknown	unknown	unknown
CP024101.1\|AWC32794.1\|2030886_2032122_-\|cytochrome-P450	unknown	unknown	gnl\|CDD\|225035
CP024101.1\|AWC32793.1\|2029331_2030615_-\|MFS-transporter	unknown	unknown	gnl\|CDD\|340863
CP024101.1\|AWC32803.1\|2043069_2043831_-\|DUF3959-domain-containing-protein	unknown	unknown	gnl\|CDD\|372477
CP024101.1\|AWC32806.1\|2047144_2047828_-\|thiaminase-II	unknown	unknown	gnl\|CDD\|381699

Protein	Function_ID	Function_description	E-value
CP024101.1\|AWC32796.1\|2032840_2033845_-\|hypothetical-protein	gnl\|CDD\|378848	pfam12395, DUF3658, Protein of unknown function. This domain family is found in bacteria, and is approximately 110 amino acids in length. The family is found in association with pfam08874. There are two completely conserved residues (D and R) that may be functionally important.	3.20111e-35
CP024101.1\|AWC32790.1\|2027199_2028054_-\|Cof-type-HAD-IIB-family-hydrolase	gnl\|CDD\|319818	cd07516, HAD_Pase, phosphatase, similar to Escherichia coli Cof and Thermotoga maritima TM0651; belongs to the haloacid dehalogenase-like superfamily. Escherichia coli Cof is involved in the hydrolysis of HMP-PP (4-amino-2-methyl-5-hydroxymethylpyrimidine pyrophosphate, an intermediate in thiamin biosynthesis), Cof also has phosphatase activity against the coenzymes pyridoxal phosphate (PLP) and FMN. Thermotoga maritima TM0651 acts as a phosphatase with a phosphorylated carbohydrate molecule as a possible substrate. Escherichia coli YbhA is also a member of this family and catalyzes the dephosphorylation of PLP, YbhA can also hydrolyze erythrose-4-phosphate and fructose-1,6-bis-phosphate. Members of this family belong to the haloacid dehalogenase-like (HAD) hydrolases, a large superfamily of diverse enzymes that catalyze carbon or phosphoryl group transfer reactions on a range of substrates, using an active site aspartate in nucleophilic catalysis. Members of this superfamily include 2-L-haloalkanoic acid dehalogenase, azetidine hydrolase, phosphonoacetaldehyde hydrolase, phosphoserine phosphatase, phosphomannomutase, P-type ATPases and many others. HAD hydrolases are found in all three kingdoms of life, and most genomes are predicted to contain multiple HAD-like proteins. Members possess a highly conserved alpha/beta core domain, and many also possess a small cap domain, the fold and function of which is variable. HAD hydrolases are sometimes referred to as belonging to the DDDD superfamily of phosphohydrolases.	4.97119e-76
CP024101.1\|AWC32806.1\|2047144_2047828_-\|thiaminase-II	gnl\|CDD\|381699	cd19364, TenA_C_BsTenA-like, TenA_C proteins similar to Bacillus subtilis TenA. This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Bacillus subtilis TenA which has been shown to be a thiaminase II, catalyzing the hydrolysis of thiamine into HMP and 5-(2-hydroxyethyl)-4-methylthiazole (THZ), within thiamine metabolism. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.	2.93311e-111
CP024101.1\|AWC34885.1\|2048146_2048794_-\|Na+/myo-inositol-cotransporter	gnl\|CDD\|236699	PRK10484, PRK10484, putative transporter; Provisional.	5.42292e-97
CP024101.1\|AWC32805.1\|2044544_2046890_-\|translation-initiation-inhibitor	gnl\|CDD\|372436	pfam13032, DUF3893, Domain of unknown function (DUF3893). This presumed domain is functionally uncharacterized. This domain family is found in bacteria, and is typically between 123 and 144 amino acids in length. There is a single completely conserved residue E that may be functionally important.	2.06227e-27
CP024101.1\|AWC32801.1\|2038632_2039535_-\|type-II-CRISPR-associated-endonuclease-Cas1	gnl\|CDD\|274694	TIGR03639, cas1_NMENI, CRISPR-associated endonuclease Cas1, subtype II/NMENI. The CRISPR-associated protein Cas1 is virtually universal to CRISPR systems. CRISPR, an acronym for Clustered Regularly Interspaced Short Palindromic Repeats, is a prokaryotic immunity system for foreign DNA, mostly from phage. CRISPR systems belong to different subtypes, distinguished by both nature of the repeats, the makeup of the cohort of associated Cas proteins, and by molecular phylogeny within the more universal Cas proteins such as this one. This model is of type EXCEPTION and provides more specific information than the EQUIVALOG model TIGR00287. It describes the Cas1 variant of the NMENI subtype of CRISPR/Cas system.	7.00879e-101
CP024101.1\|AWC32802.1\|2039524_2042758_-\|type-II-CRISPR-RNA-guided-endonuclease-Cas9	gnl\|CDD\|226044	COG3513, COG3513, Predicted CRISPR-associated nuclease, contains McrA/HNH-nuclease and RuvC-like nuclease domain [Defense mechanisms].	0
CP024101.1\|AWC32807.1\|2049332_2049833_-\|N-acetyltransferase	gnl\|CDD\|273701	TIGR01575, rimI, ribosomal-protein-alanine acetyltransferase. Members of this model belong to the GCN5-related N-acetyltransferase (GNAT) superfamily. This model covers prokarotes and the archaea. The seed contains a characterized accession for Gram negative E. coli. An untraceable characterized accession (PIR\|S66013) for Gram positive B. subtilis scores well (205.0) in the full alignment. Characterized members are lacking in the archaea. Noise cutoff (72.4) was set to exclude M. loti paralog of rimI. Trusted cutoff (80.0) was set at next highest scoring member in the mini-database. [Protein synthesis, Ribosomal proteins: synthesis and modification].	8.0744e-08
CP024101.1\|AWC32792.1\|2029103_2029259_-\|DUF1540-domain-containing-protein	gnl\|CDD\|377869	pfam07561, DUF1540, Domain of Unknown Function (DUF1540). This family has four conserved cysteines, which is suggestive of a metal binding function.	5.52411e-11
CP024101.1\|AWC32800.1\|2038319_2038628_-\|CRISPR-associated-endonuclease-Cas2	gnl\|CDD\|226043	COG3512, COG3512, CRISPR-associated protein, Cas2 homolog [Defense mechanisms].	2.72822e-35
CP024101.1\|AWC32799.1\|2037465_2037630_-\|hypothetical-protein	gnl\|CDD\|275209	TIGR04416, hypothetical_protein, group II intron reverse transcriptase/maturase. Members of this protein family are multifunctional proteins encoded in most examples of bacterial group II introns. These group II introns are mobile selfish genetic elements, often with multiple highly identical copies per genome. Member proteins have an N-terminal reverse transcriptase (RNA-directed DNA polymerase) domain (pfam00078) followed by an RNA-binding maturase domain (pfam08388). Some members of this family may have an additional C-terminal DNA endonuclease domain that this model does not cover. A region of the group II intron ribozyme structure should be detectable nearby on the genome by Rfam model RF00029. [Mobile and extrachromosomal element functions, Other].	5.03974e-06
CP024101.1\|AWC32794.1\|2030886_2032122_-\|cytochrome-P450	gnl\|CDD\|225035	COG2124, CypX, Cytochrome P450 [Secondary metabolites biosynthesis, transport, and catabolism].	6.28682e-103
CP024101.1\|AWC32791.1\|2028255_2028834_-\|cysteine-dioxygenase	gnl\|CDD\|380416	cd10548, cupin_CDO, cysteine dioxygenase, cupin domain. This family contains cysteine dioxygenase (CDO; EC 1.13.11.20), which catalyzes the conversion of cysteine to cysteine sulfinic acid, the first step in the biosynthesis of essential oxidized cysteine metabolites such as sulfate, hypotaurine, and taurine. CDO also plays an important role in the regulation of intracellular cysteine levels in mammals; CDO expression is altered in cancer cells, and abnormal or deficient CDO activity has been linked to Parkinson's disease, Alzheimer's disease, and rheumatoid arthritis. CDO is an iron-dependent thiol dioxygenase that uses molecular oxygen to oxidize the sulfhydryl group of cysteine to generate cysteine sulfinic acid. The CDO active site contains an amino acid-derived cofactor. These enzymes are found in prokaryotes as well as eukaryotes and belong to the cupin superfamily with a conserved "jelly roll-like" beta-barrel fold capable of homodimerization.	3.59854e-25
CP024101.1\|AWC32793.1\|2029331_2030615_-\|MFS-transporter	gnl\|CDD\|340863	cd06173, MFS_MefA_like, Macrolide efflux protein A and similar proteins of the Major Facilitator Superfamily of transporters. This family is composed of Streptococcus pyogenes macrolide efflux protein A (MefA) and similar transporters, many of which remain uncharacterized. Some members may be multidrug resistance (MDR) transporters, which are drug/H+ antiporters (DHAs) that mediate the efflux of a variety of drugs and toxic compounds, conferring resistance to these compounds. MefA confers resistance to 14-membered macrolides including erythromycin and to 15-membered macrolides. It functions as an efflux pump to regulate intracellular macrolide levels. The MefA-like family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.	4.71525e-59
CP024101.1\|AWC32803.1\|2043069_2043831_-\|DUF3959-domain-containing-protein	gnl\|CDD\|372477	pfam13105, DUF3959, Protein of unknown function (DUF3959). This family of proteins is functionally uncharacterized. This family of proteins is found in bacteria. Proteins in this family are approximately 260 amino acids in length.	6.19952e-160

>CP024101.1|AWC32799.1|2037465_2037630_-|hypothetical-protein
MGQFSVTKVGQIWLTIYKYLIGWCGYNALSDTPSICKERDKWIRRRLRMVIWKQ
>CP024101.1|AWC32798.1|2034466_2034769_-|hypothetical-protein
MKKSKAERKRDLEIVNLYHKKLTEEELEKLSVKFIEWKEGKLPYCELTEHIHECHKKNQIIWTRFHNMNDEFLIFQAKKELNLFSERDMEKEQYKRWNTF
>CP024101.1|AWC32797.1|2033857_2034391_-|hypothetical-protein
MQLIQCTKKLADMMELKTEKVDQRESNNLHSWHANLFTINRKKCVIVMNNVTRYHFIIYGVTKKDLRNFEALFQKNLVTNLLSDGVEPEVIEHYVKSSSPMQYAATNNRSILSQMNDAVFMVDHYFYAELVEKQKPFINIEKLNQNLNKTVMLKIPKYPADMMRDALNQHLIMSKNE
>CP024101.1|AWC32796.1|2032840_2033845_-|hypothetical-protein
MRDQLRKIIDNMSEEEVKSLLRLVLLQLELVAEHRDEEMIHMVAEIPKQLIESQKPTLHIKDSEHVHIAFGDSSASCLKHTLKKEGFHQDYVISLSDIFSVGPIFHLDDRKGQVARQEWLSSNLSTGDFYFEEEYLPRFLSSLEEMRSISKDTSVTIWKADNAHEHVGLCFALAMLKDKKNIRIINASEAQKELLNKEYDICGTGELAPEDIAVILEQYKEKECLKEEARADLTKEWNALSTNTALLRIWKDGTVHSVVEDYFDEYIVRCAKEIGADKGFSKAAHVIGEVLGRVEQLVGDVFLEYRLQTLIKQNIFQAKGSLKAMRYYSVKLNR
>CP024101.1|AWC32795.1|2032275_2032686_-|hypothetical-protein
MYNYPNYPGIGYDQTMYSQYQGYGEEAMEPRESDYEEYRPDDAEDAPTSPPPSQAPSVPSTFASPVQSSNIRSWVCNCLGRWGYLRLHRPGPFGRDLWFFPTEVRRGGVSGYTWQAGRRRKVSYRYQQISNFMCFA
>CP024101.1|AWC32794.1|2030886_2032122_-|cytochrome-P450
MSVKNKVGRNIKDGIKLASPEFKEYAYEIYKESRKLQPILFVYKGELGTEWLITRYEDALSLLKDSRLKKNPENIFFQEKLKSLFSIENSDYLTKHMLNADPPDHNRLRALVQKTFTPKMISQLHNRIQHIADTLLDHVEQKRSFHFIRDFSFPLPIIVISEMLGIPKEDQAKFRIWSHAVIDSPEIPEEKIENEEKLSEFITYLQYLVDLKRKEPKEDLISRLIQAESEGGQLSAAELYSMIMLLIVAGHETTVNLMTNTVLALLENPDQLQLLKERPELIDSAIEEGLRYYSPVEVTTARWAAEPFHIHDKVIQKGDMVTISLASANRDETVFVNPDVFNITRENNRHIAFGHGGHFCLGAPLARLEAKIAIATLLKRMPRLRIKGKREDIKWEGNYLMRSLKELPLTF
>CP024101.1|AWC32793.1|2029331_2030615_-|MFS-transporter
MSVSAETNPKVGMSQVLKNRFVQGILISALCLQIGIWVRNFAVLLYVMEMTKGNAFAISMISVAEFAPIFIFSFIGGTFADRWKPRKTMIWCEALSSLSVFAVLLTLMFGTWKVVFFVTLISAILSQFSQPSGMKLFKQHLSAEEIQLAMSLYQTTFAIFMILGPILGTFVFQSFGIYISIVVTGVAFLLAAIVLLFLPKDVEMHAEKKQVSLLQEMADGIKYVLGKKALTLLGICFMAAGLGIGLIQPLGIFIVTEQLGLSKESLQWLLTVNGAGMIIGGALAMIFAKNVAPQKMLMIGLLAQAIGIGVIGFSTNLWITLTAQLFSGLALPCIQIGINTLIIQNSDTDFIGRVNGILSPLFTGSMVLTMSIAGSLKEMFSLSMLYEGTAVLFIVGLFVILPIYHLKPAVRAENQPAKEGSAIQQES
>CP024101.1|AWC32792.1|2029103_2029259_-|DUF1540-domain-containing-protein
MAQDVLCEVNNCKYWGNGNKCNADAIYVVSHKGKQASKTEETDCKTFTPEA
>CP024101.1|AWC32791.1|2028255_2028834_-|cysteine-dioxygenase
MLTCRKSELFQQFIHNVTASIENSKNEETIVCTIEKLLEKLLETKAWLPLEKQKANRNQYARHLLYKDPLDRFEVLALVWRDGQATPLHDHDGTWGVEGVFSGRIKVKNFLKIKQLEESIFQLKYLGHLYLGEGKTDKIIPPADCHILEIEPNEQVITIHIYGKKLEKFKVFIPTERKDIYRCKMKYIQYSS
>CP024101.1|AWC32790.1|2027199_2028054_-|Cof-type-HAD-IIB-family-hydrolase
MKLIAIDLDGTLLSKNKMISKENAEAIRKCQEEGHVVAICTGRSIVDIERLLQEVNLQCPIIAENGAVIYKDKTMLKRYAIQNEHALDIVEYLEEQGMYYQLYTDKGVYVPKYGEENIRKEMEVIRNLDINISEEEVENIAALYLEHTAFHSIESCKSIINKELYVHKFLPFSYDQQKLKRLKEKFQHNSELIISASYWHNLEINHCDAQKGNGLYTLAEYFNIPFENTVAIGDGLNDVSMMQKANVSIAMGNAVNELKAMCHYETLTNEEHGVAHALYHYVIA
>CP024101.1|AWC32800.1|2038319_2038628_-|CRISPR-associated-endonuclease-Cas2
MRILVFFDLPVMTKAERKAYTRFRKFLLKDGYTMLQFSVYTRVCNGEEGAQKHLRRLRQNIPNVNGAIRSLKVTEKQFESMDILLGVQTIDEKLGTNKTDFF
>CP024101.1|AWC32801.1|2038632_2039535_-|type-II-CRISPR-associated-endonuclease-Cas1
MAFRHIVIRNPSKLSTCDEQLLIKQEETVRIPLEDICTITIEDPAITITAALLSKCVEYHVELIVCDWKRLPAGILQPFHRHSRQKAVLDVQLGLGKPFKKRIWQQIVIRKLENQARCLELANKGEDAQKLYSISKSVESGDRTNREAYGAKVYFTALFGPNFYRREDDVRNISLNYGYSLVRSLTARALVRYGFNPSLGIFHDSQTNAFNLADDFMEVLRPFVDIIVALNVSDETEWDSKMREKLFSVLNIEAVWKEERQSITNGVEQMIKSYVSACRQNDASLLLLPELIALRQHTYE
>CP024101.1|AWC32802.1|2039524_2042758_-|type-II-CRISPR-RNA-guided-endonuclease-Cas9
MKYSIGLDIGIASVGWAVINEDIERIEDLGVRIFDVAEQPKTGASLSTPRRLARSTRKRLRRRRHRVERVRKFMIQKGLLTQVQMEQLYDWKDGDLDIWLVRVNALERKLSDREFARILVHFAKNRGFQSNRKSELQEEDNGAVLSAVQENKRLMEEKGYQTISEMLLYDEKFEGTKRNKGGEYTHVVARSDLKREIQLIFEKQRAYGHAFATMENEDKFLQIWSSQRPFATKDDIAKKIGNCTFEPKEKRAPKSTYTFERFRALDKLNRLRILSGTSPARSLTQEERDLILNSLFSKKEVKYKELRKLLKLNDEQRFNEIFYDPNEKIEKNENRTFLSLESQYKIRKILQKIEGKSLNDTYRPVDLDTIAYALTVFKDDQDIRDYLQNQYVASNGKKELNLANRKYNDELIEELLHLSFTKFGHLSLKALRNILPFMEQGIPYHQACEKAGYQFNKKVNREKQKLLPPVPAKDIVNPVVIRSLSQVRKVINGIIKRYGSPSSIYIELAREMGRGYKERKDLEKQYNKNRQMNEGAKKHILELRPEFGDPRGHDILKYKLWQEQDCRCAYSFKSIPIKQLFEIGYAEVDHIIPYSRSFDDSNNNKVLVLAAENQNKQNRTPYEWFGHDETKWQEFTSLVTTMKVSKKKKSLLLKKNFDEEQAEEFRSRHLNDTRYITRYIKTFIEDNLQFRIEEGKKTYVHTVNGAYTSLMRKRWGFNKNRQENALHHALDAVIVAVSLPFKHKVRAYFKRREEYAHNQLKREGEFFPEPWPGFIHELEIRMMQDSENIKLAMESLNLKTYDESFIKELKPIFVSRMPKRAMKGQIHEETLKRVRGRTDDGYIRTVKKVKLDQIPFDKNGDFPMFGKETDMKTYEAIKSRYLESGKDKKKAFNTPLYKPAKNPENAPIIRSIKIEEKANRVVMLDEKTAADNASIVRTEVFRHKETGNYFLTPVYVADVLAKKVPDRLITTKKPYSEWRKITDEYEFLFSLYPNDLIRVKMPKEKETKTHTGEKHVWQENFMYFKGVHSGSAGVRMIDHMNSFEDEIGVRKLVVFEKYQVDPLGRMNKVHGEKRHGI
>CP024101.1|AWC32803.1|2043069_2043831_-|DUF3959-domain-containing-protein
MKKLDVLLMLLSGLFPVAGIMKQIPLPLSLILGGLLFFTSFGSYYTKQTSSRVCSWLAYTIFITFLMGIWDQYVTMSSLLANAKIAACIALLPAILCFRTYGITFGLLALWGALLWDVKEVQSLAVLGRMMNLLTTEKMYLLLLIAGFLVGGLFANGFRRKNDKFKQETPNVLKQKKKRMRPTFHIRIPRLPKLRMKMLTFGRTPTQSKDKRPYESMREENIHAIQQEPQHEQQEPTTLGQTRMERRRNRYHV
>CP024101.1|AWC32804.1|2044087_2044396_-|hypothetical-protein
MFFIVYIFTNLPLSLIVDNIPKALKSLDLIQTSKGWIPFLFDAGKTYILIMTIDYFMESITISWQGVFLFAIIRGSIGLKIKKDDPEPPSYSEVTKSLKNNE
>CP024101.1|AWC32805.1|2044544_2046890_-|translation-initiation-inhibitor
METIQLLAFKDVIDPLFEQTVFYVQWPKHWSSLLLEHNRPYELIKKFKLLNKRLYIMFSDILFIEHDPYKLAEDSLWIICLKPLSSEQLEYICRQWYAYIHNWKPTEMPGEVLLEWQPSAISKLSLLHDKKTFYIWVPALISHLFCERALHLSIGNKQYEEVPFYPLREQNRCEAMSEPIQDKKTKEYFSYIYRFELITRGIENMFLLKVSIGKRRFYQTANQYANYMIMKKRKGTVLVSNCHNEKDKFVTLKIEDARFGVNWSKAYRELQNVFMLKKQLDLVKIMQSPKKYIKGKRIRALVIYDENIFKVPGTKIKRGISRREKEVLLQAFHQRFPHCTYIPLCKNVTINQNDELFPLYIREEMKEITLELWSFQRLTEIERILFEEGLILEKRANHSYILNSKPQIILNIVTYNPKIIKQHPYRMDYCHKYKEDRIQSVIRAISSIPIQSFQTSLSLIEVEKCDGREKIDPKQVIREAFARTNRISQFIERLDGEEIKKEHIVRAIFSLLEKIGFRKRSWESMHPSHTYVSLSIEEIYNNQFVPILSKINEGEIVYKLYGSHEWRDFTSILLNVSAHETFLPQPSKRNDIGIQFKKYITEELTAIAEEAKKENKQVYFLIDASMREYWIESLQNEHMDLHDLPYISEEFQSLSNVTAIRINNQYDVPNYIMPLEKECDETELYVDQSGLYYSVNIYPLNDTGWKEQRILEIVVLGAKQEERDQIAKIITYMCKMSIIPGERPFIPFPMQMVKLIKKYITDIDLWQSSDELDTDWLIENE
>CP024101.1|AWC32806.1|2047144_2047828_-|thiaminase-II
MSFSQLLRQEVAPIWQASFEHPFIKELGEGTLDLASFRYYVLQDSYYLSHFARVQTLGAAKAIELKTTARMAHHAQNTYEAELSLHENFAEKLGITEEEKASFIPAPTAYAYTSHMYRAAYEGHLGDIIASILPCYWLYYEIGERLKECKPEEPIYQAWISAYGSDWFRTLVEEQIMRLDQIAETVTESDRKRMKQHFIISSQYEYSFWEMAYTLEKWPVEEKIATR
>CP024101.1|AWC34885.1|2048146_2048794_-|Na+/myo-inositol-cotransporter
MAYPMLVIKILPIALSGFFAAVLFGAILSSFNGALNSSITLFTLDFYKPIFKKEASEKELVKVGRIFAITLGLIAVIVSPFILYAPSGLYNYLQEMFGFYNVPIIAAVVVGFFSKKVPASAPKIALVFHIILYALSKVFLGSINFLYILSVLFPACVIVMLVIGKLKPRETDFELFDANKVDLKPWKYAKVFSIFLIIFMLGAYVLFSSVGLAAQ
>CP024101.1|AWC34886.1|2049080_2049248_-|hypothetical-protein
MSIIALVSILLWGAVTHELSKPSKKQNNRKIITLTSLGCLSTLVIIISLIQNLSF
>CP024101.1|AWC32807.1|2049332_2049833_-|N-acetyltransferase
MSIHTENIKLVPYKEEYKEKIATFTLPSEQVQFTANPSDLLEKAKEDITKHVVVIVDQDVPVGIFALQTGERVTEYTNNPNALLLVSFSVDYEKQGKGYAKRGLRLLQEYVTAYFPEINEVVLAVNERNIPAQKVYLKVGFEDRGQRKMGPIGRQLILYLSIKKQE

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Crispr_ID: CP024101_3

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

CP024101_3

2165637-2166389

Unclear

I-A

Consensus_repeat	Method
CTTTATATCCCACTACGTTCAGATAAAAC	CRISPRCasFinder
CTTTATATCCCACTACGTTCAGATAAAAC	CRT
CTTTATATCCCACTACGTTCAGATAAAAC	PILER-CR

11 spacers

cas6,cas8b2,cas7,cas5,cas3,cas4,cas1,cas2

The CRISPR arrays of CP024101_3

>merge|CP024101|3|2165637-2166389|CRISPRCasFinder,CRT,PILER-CR
TTATCTTATCCACTACGTTCAGATAAAACTTTCTAACAATGTCCAAAAACCATTGAATACATTGGATTTATATCCCACTACGTTCAGATAAAACTAATTCATCAAATTCTTTTCCGCCTGCGCTAATTACCTTTATATCCCACTACGTTCAGATAAAACGTACTCATTAAACAAAAATAAATCTATAAAATCCGACTTTATATCCCACTACGTTCAGATAAAACAAAAGCGTGGTCGTGAAACTGGTAAAGAGGTAACGCTCTTTATATCCCACTACGTTCAGATAAAACCGTCGCATTGGTTCCGAATTGGACAAGTTCACCGCCTTTATATCCCACTACGTTCAGATAAAACCCGTTGATTATTTACTTGGATTAACTTCTGCAAGACGCTTTATATCCCACTACGTTCAGATAAAACCAAGTCGTGGACAGTCTACCAACCACGATACGACTTTCTTTATATCCCACTACGTTCAGATAAAACTACCACAAACCTGTCACGTATCATTTTCATCAGCAATGACTTTATATCCCACTACGTTCAGATAAAACTGAATCCGAGATTGGAATGGTGTTTTGTAATGTGTCAACTTTATATCCCACTACGTTCAGATAAAACTTATTTTCTCAAAGTGTGAAATAAACTCGCGAAAAATCTTTATATCCCACTACGTTCAGATAAAACCAAGTCGTGGACAGTCTACCCACAACGATTCGACTTTCTTTATATCCCACTACGTTCAGATAAAAC

>CP024101|3|3|2165637-2166389|CRISPRCasFinder
TTATCTTATCCACTACGTTCAGATAAAAC	TTTCTAACAATGTCCAAAAACCATTGAATACATTGG
ATTTATATCCCACTACGTTCAGATAAAAC	TAATTCATCAAATTCTTTTCCGCCTGCGCTAATTAC
CTTTATATCCCACTACGTTCAGATAAAAC	GTACTCATTAAACAAAAATAAATCTATAAAATCCGA
CTTTATATCCCACTACGTTCAGATAAAAC	AAAAGCGTGGTCGTGAAACTGGTAAAGAGGTAACGCT
CTTTATATCCCACTACGTTCAGATAAAAC	CGTCGCATTGGTTCCGAATTGGACAAGTTCACCGC
CTTTATATCCCACTACGTTCAGATAAAAC	CCGTTGATTATTTACTTGGATTAACTTCTGCAAGACG
CTTTATATCCCACTACGTTCAGATAAAAC	CAAGTCGTGGACAGTCTACCAACCACGATACGACTTT
CTTTATATCCCACTACGTTCAGATAAAAC	TACCACAAACCTGTCACGTATCATTTTCATCAGCAATGA
CTTTATATCCCACTACGTTCAGATAAAAC	TGAATCCGAGATTGGAATGGTGTTTTGTAATGTGTCAA
CTTTATATCCCACTACGTTCAGATAAAAC	TTATTTTCTCAAAGTGTGAAATAAACTCGCGAAAAAT
CTTTATATCCCACTACGTTCAGATAAAAC	CAAGTCGTGGACAGTCTACCCACAACGATTCGACTTT
CTTTATATCCCACTACGTTCAGATAAAAC

>CP024101|3|2|2165637-2166389|CRT
TTATCTTATCCACTACGTTCAGATAAAAC	TTTCTAACAATGTCCAAAAACCATTGAATACATTGG
ATTTATATCCCACTACGTTCAGATAAAAC	TAATTCATCAAATTCTTTTCCGCCTGCGCTAATTAC
CTTTATATCCCACTACGTTCAGATAAAAC	GTACTCATTAAACAAAAATAAATCTATAAAATCCGA
CTTTATATCCCACTACGTTCAGATAAAAC	AAAAGCGTGGTCGTGAAACTGGTAAAGAGGTAACGCT
CTTTATATCCCACTACGTTCAGATAAAAC	CGTCGCATTGGTTCCGAATTGGACAAGTTCACCGC
CTTTATATCCCACTACGTTCAGATAAAAC	CCGTTGATTATTTACTTGGATTAACTTCTGCAAGACG
CTTTATATCCCACTACGTTCAGATAAAAC	CAAGTCGTGGACAGTCTACCAACCACGATACGACTTT
CTTTATATCCCACTACGTTCAGATAAAAC	TACCACAAACCTGTCACGTATCATTTTCATCAGCAATGA
CTTTATATCCCACTACGTTCAGATAAAAC	TGAATCCGAGATTGGAATGGTGTTTTGTAATGTGTCAA
CTTTATATCCCACTACGTTCAGATAAAAC	TTATTTTCTCAAAGTGTGAAATAAACTCGCGAAAAAT
CTTTATATCCCACTACGTTCAGATAAAAC	CAAGTCGTGGACAGTCTACCCACAACGATTCGACTTT
CTTTATATCCCACTACGTTCAGATAAAAC

>CP024101|3|2|2165703-2166389|PILER-CR
TTTATATCCCACTACGTTCAGATAAAACT	AATTCATCAAATTCTTTTCCGCCTGCGCTAATTACC
TTTATATCCCACTACGTTCAGATAAAACG	TACTCATTAAACAAAAATAAATCTATAAAATCCGAC
TTTATATCCCACTACGTTCAGATAAAACA	AAAGCGTGGTCGTGAAACTGGTAAAGAGGTAACGCTC
TTTATATCCCACTACGTTCAGATAAAACC	GTCGCATTGGTTCCGAATTGGACAAGTTCACCGCC
TTTATATCCCACTACGTTCAGATAAAACC	CGTTGATTATTTACTTGGATTAACTTCTGCAAGACGC
TTTATATCCCACTACGTTCAGATAAAACC	AAGTCGTGGACAGTCTACCAACCACGATACGACTTTC
TTTATATCCCACTACGTTCAGATAAAACT	ACCACAAACCTGTCACGTATCATTTTCATCAGCAATGAC
TTTATATCCCACTACGTTCAGATAAAACT	GAATCCGAGATTGGAATGGTGTTTTGTAATGTGTCAAC
TTTATATCCCACTACGTTCAGATAAAACT	TATTTTCTCAAAGTGTGAAATAAACTCGCGAAAAATC
TTTATATCCCACTACGTTCAGATAAAACC	AAGTCGTGGACAGTCTACCCACAACGATTCGACTTTC
TTTATATCCCACTACGTTCAGATAAAAC

Protein	Signature genes	Signature genes Name	Protein_function
CP024101.1\|AWC32900.1\|2161309_2162203_-\|type-I-B-CRISPR-associated-protein-Cas7/Cst2/DevR	cas7	cd09687_cas7_CAS-I-B	gnl\|CDD\|187818
CP024101.1\|AWC32903.1\|2164856_2165108_-\|hypothetical-protein	unknown	unknown	unknown
CP024101.1\|AWC32895.1\|2156530_2156794_-\|CRISPR-associated-endonuclease-Cas2	cas2	cd09725_cas2_CAS-I:CAS-II:CAS-III:CAS-V	gnl\|CDD\|187856
CP024101.1\|AWC32898.1\|2158335_2160561_-\|CRISPR-associated-helicase/endonuclease-Cas3	cas3	COG1203_cas3_CAS-I	gnl\|CDD\|273707
CP024101.1\|AWC32901.1\|2162195_2163944_-\|type-I-B-CRISPR-associated-protein-Cas8b1/Cst1	cas8b2	cd09665_cas8b2_CAS-I-B	gnl\|CDD\|187796
CP024101.1\|AWC32894.1\|2154724_2155108_-\|VOC-family-protein	unknown	unknown	gnl\|CDD\|319924
CP024101.1\|AWC32897.1\|2157775_2158273_-\|CRISPR-associated-protein-Cas4	cas4	pfam01930_cas4_CAS-I	gnl\|CDD\|366863
CP024101.1\|AWC32912.1\|2172066_2172534_-\|NUDIX-domain-containing-protein	unknown	unknown	gnl\|CDD\|240044
CP024101.1\|AWC32904.1\|2166675_2167002_-\|antibiotic-biosynthesis-monooxygenase	unknown	unknown	gnl\|CDD\|225209
CP024101.1\|AWC32910.1\|2170077_2170401_-\|PadR-family-transcriptional-regulator	unknown	unknown	gnl\|CDD\|163261
CP024101.1\|AWC32908.1\|2169056_2169302_+\|DNA-alkylation-repair-protein	unknown	unknown	unknown
CP024101.1\|AWC32907.1\|2168120_2168687_-\|N-acetyltransferase	unknown	unknown	gnl\|CDD\|224584
CP024101.1\|AWC32906.1\|2167812_2168142_-\|hypothetical-protein	unknown	unknown	gnl\|CDD\|169051
CP024101.1\|AWC32896.1\|2156798_2157782_-\|type-I-B-CRISPR-associated-endonuclease-Cas1	cas1	cd09722_cas1_CAS-I-B	gnl\|CDD\|274695
CP024101.1\|AWC32899.1\|2160614_2161313_-\|type-I-B-CRISPR-associated-protein-Cas5	cas5	cd09658_cas5_CAS-I-B	gnl\|CDD\|187789
CP024101.1\|AWC32909.1\|2169539_2170076_-\|DUF2812-domain-containing-protein	unknown	unknown	gnl\|CDD\|378589
CP024101.1\|AWC32911.1\|2171002_2171986_-\|DUF4003-domain-containing-protein	unknown	unknown	gnl\|CDD\|379047
CP024101.1\|AWC32902.1\|2163943_2164645_-\|CRISPR-associated-endoribonuclease-Cas6	cas6	pfam01881_cas6_CAS-I:CAS-III	gnl\|CDD\|187783
CP024101.1\|AWC32905.1\|2167343_2167688_+\|hypothetical-protein	unknown	unknown	unknown
CP024101.1\|AWC32913.1\|2172892_2173138_-\|hypothetical-protein	unknown	unknown	gnl\|CDD\|365903

Protein	Function_ID	Function_description	E-value
CP024101.1\|AWC32900.1\|2161309_2162203_-\|type-I-B-CRISPR-associated-protein-Cas7/Cst2/DevR	gnl\|CDD\|187818	cd09687, Cas7_I-C, CRISPR/Cas system-associated RAMP superfamily protein Cas7. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and associated Cas proteins comprise a system for heritable host defense by prokaryotic cells against phage and other foreign DNA; Cas7 is a RAMP superfamily protein; Subunit of the Cascade complex; also known as Cst2/DevR family.	4.74396e-53
CP024101.1\|AWC32901.1\|2162195_2163944_-\|type-I-B-CRISPR-associated-protein-Cas8b1/Cst1	gnl\|CDD\|187796	cd09665, Cas8a1_I-A, CRISPR/Cas system-associated protein Cas8a1. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and associated Cas proteins comprise a system for heritable host defense by prokaryotic cells against phage and other foreign DNA; Large proteins, some contain Zn-finger domain; signature gene for I-A subtype; also known as CXXC_CXXC family.	3.46239e-10
CP024101.1\|AWC32895.1\|2156530_2156794_-\|CRISPR-associated-endonuclease-Cas2	gnl\|CDD\|187856	cd09725, Cas2_I_II_III, CRISPR/Cas system-associated protein Cas2. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and associated Cas proteins comprise a system for heritable host defense by prokaryotic cells against phage and other foreign DNA; Cas2 is present in majority of CRISPR/Cas systems along with Cas1; RNAse specific to U-rich regions; Possesses an RRM/ferredoxin fold.	2.02502e-27
CP024101.1\|AWC32898.1\|2158335_2160561_-\|CRISPR-associated-helicase/endonuclease-Cas3	gnl\|CDD\|273707	TIGR01587, CRISPR-associated_endonuclease/helicase_Cas3, CRISPR-associated helicase Cas3. This model represents the highly conserved core region of an alignment of Cas3, a protein found in association with CRISPR repeat elements in a broad range of bacteria and archaea. Cas3 appears to be a helicase, with regions found by pfam00270 (DEAD/DEAH box helicase) and pfam00271 (Helicase conserved C-terminal domain). Some but not all members have an N-terminal HD domain region (pfam01966) that is not included within this model.	1.52511e-63
CP024101.1\|AWC32894.1\|2154724_2155108_-\|VOC-family-protein	gnl\|CDD\|319924	cd07263, VOC_like, uncharacterized subfamily of vicinal oxygen chelate (VOC) family. The vicinal oxygen chelate (VOC) superfamily is composed of structurally related proteins with paired beta.alpha.beta.beta.beta motifs that provide a metal coordination environment with two or three open or readily accessible coordination sites to promote direct electrophilic participation of the metal ion in catalysis. VOC domain is found in a variety of structurally related metalloproteins, including the bleomycin resistance protein, glyoxalase I, and type I ring-cleaving dioxygenases. A bound metal ion is required for protein activities for the members of this superfamily. A variety of metal ions have been found in the catalytic centers of these proteins including Fe(II), Mn(II), Zn(II), Ni(II) and Mg(II). The protein superfamily contains members with or without domain swapping. The proteins of this family share three conserved metal binding amino acids with the type I extradiol dioxygenases, which shows no domain swapping.	1.51641e-44
CP024101.1\|AWC32897.1\|2157775_2158273_-\|CRISPR-associated-protein-Cas4	gnl\|CDD\|366863	pfam01930, Cas_Cas4, Domain of unknown function DUF83. This domain has no known function. The domain contains three conserved cysteines at its C-terminus.	7.16543e-58
CP024101.1\|AWC32912.1\|2172066_2172534_-\|NUDIX-domain-containing-protein	gnl\|CDD\|240044	cd04688, Nudix_Hydrolase_29, Members of the Nudix hydrolase superfamily catalyze the hydrolysis of NUcleoside DIphosphates linked to other moieties, X. Enzymes belonging to this superfamily require a divalent cation, such as Mg2+ or Mn2+, for their activity and contain a highly conserved 23-residue nudix motif (GX5EX7REUXEEXGU, where U = I, L or V), which functions as a metal binding and catalytic site. Substrates of nudix hydrolases include intact and oxidatively damaged nucleoside triphosphates, dinucleoside polyphosphates, nucleotide-sugars and dinucleotide enzymes. These substrates are metabolites or cell signaling molecules that require regulation during different stages of the cell cycle or during periods of stress. In general, the role of the nudix hydrolase is to sanitize the nucleotide pools and to maintain cell viability, thereby serving as surveillance & "house-cleaning" enzymes. Substrate specificity is used to define families within the superfamily. Differences in substrate specificity are determined by the N-terminal extension or by residues in variable loop regions. Mechanistically, substrate hydrolysis occurs by a nucleophilic substitution reaction, with variation in the numbers and roles of divalent cations required.	6.58437e-49
CP024101.1\|AWC32904.1\|2166675_2167002_-\|antibiotic-biosynthesis-monooxygenase	gnl\|CDD\|225209	COG2329, COG2329, Uncharacterized enzyme involved in biosynthesis of extracellular polysaccharides [General function prediction only].	5.76195e-19
CP024101.1\|AWC32910.1\|2170077_2170401_-\|PadR-family-transcriptional-regulator	gnl\|CDD\|163261	TIGR03433, padR_acidobact, transcriptional regulator, Acidobacterial, PadR-family. Members of this protein family are putative transcriptional regulators of the PadR family, as found in species of the Acidobacteria. This family of proteins has expanded greatly in this lineage, and where it regularly is found in the vicinity of a putative transporter protein [Regulatory functions, DNA interactions].	5.02854e-08
CP024101.1\|AWC32907.1\|2168120_2168687_-\|N-acetyltransferase	gnl\|CDD\|224584	COG1670, RimL, Acetyltransferases, including N-acetylases of ribosomal proteins [Translation, ribosomal structure and biogenesis].	9.21142e-46
CP024101.1\|AWC32906.1\|2167812_2168142_-\|hypothetical-protein	gnl\|CDD\|169051	PRK07667, PRK07667, uridine kinase; Provisional.	2.41618e-46
CP024101.1\|AWC32896.1\|2156798_2157782_-\|type-I-B-CRISPR-associated-endonuclease-Cas1	gnl\|CDD\|274695	TIGR03641, cas1_HMARI, CRISPR-associated endonuclease Cas1, subtype I-B/HMARI/TNEAP. The CRISPR-associated protein Cas1 is virtually universal to CRISPR systems. CRISPR, an acronym for Clustered Regularly Interspaced Short Palindromic Repeats, is prokaryotic immunity system for foreign DNA, mostly from phage. CRISPR systems belong to different subtypes, distinguished by both nature of the repeats, the makeup of the cohort of associated Cas proteins, and by molecular phylogeny within the more universal Cas proteins such as this one. This model is of type EXCEPTION and provides more specific information than the EQUIVALOG model TIGR00287. It describes Cas1 subgroup that includes Cas1 proteins of the related HMARI and TNEAP subtypes of CRISPR/Cas system.	5.83678e-169
CP024101.1\|AWC32899.1\|2160614_2161313_-\|type-I-B-CRISPR-associated-protein-Cas5	gnl\|CDD\|187789	cd09658, Cas5_I-B, CRISPR/Cas system-associated RAMP superfamily protein Cas5. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and associated Cas proteins comprise a system for heritable host defense by prokaryotic cells against phage and other foreign DNA; Cas5 is a RAMP superfamily protein; Subunit of the Cascade complex.	3.28572e-29
CP024101.1\|AWC32909.1\|2169539_2170076_-\|DUF2812-domain-containing-protein	gnl\|CDD\|378589	pfam11193, DUF2812, Protein of unknown function (DUF2812). This is a bacterial family of uncharacterized proteins, however some members of this family are annotated as membrane proteins.	5.25447e-33
CP024101.1\|AWC32911.1\|2171002_2171986_-\|DUF4003-domain-containing-protein	gnl\|CDD\|379047	pfam13170, DUF4003, Protein of unknown function (DUF4003). This family of proteins is functionally uncharacterized. This family of proteins is found in bacteria. Proteins in this family are typically between 327 and 345 amino acids in length.	9.46549e-103
CP024101.1\|AWC32902.1\|2163943_2164645_-\|CRISPR-associated-endoribonuclease-Cas6	gnl\|CDD\|187783	cd09652, Cas6-I-III, CRISPR/Cas system-associated RAMP superfamily protein Cas6. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and associated Cas proteins comprise a system for heritable host defense by prokaryotic cells against phage and other foreign DNA; Cas6 is an endoribonuclease that generates crRNAs, predicted subunit of Cascade complex; RAMP superfamily protein; Possesses double RRM/ferredoxin fold.	1.0641e-22
CP024101.1\|AWC32913.1\|2172892_2173138_-\|hypothetical-protein	gnl\|CDD\|365903	pfam00144, Beta-lactamase, Beta-lactamase. This family appears to be distantly related to pfam00905 and PF00768 D-alanyl-D-alanine carboxypeptidase.	0.00258085

>CP024101.1|AWC32903.1|2164856_2165108_-|hypothetical-protein
MAGTNRFLQVNVRFLEAFTYIVDGGRLFIIMKWTFSVPPFVASSFSVEVLVTNRVTLLTDLVFFHFFDASSVYIVHSISNDIR
>CP024101.1|AWC32902.1|2163943_2164645_-|CRISPR-associated-endoribonuclease-Cas6
MRLHIKLQTNRFPISYRMMMVSFIKEALKLSDAAYFQQIYQESKANRPFSYGVYIHDYKLNATEFHIKGYVGLTITSPDAQFMLHFYNGLMKMNDFRYKGFSLKRMKVQMVNSADINQNHVYFKTLSPLLIRDKHAKPIFIDDDKFEKELNYISDTVLKEFRGYGLKQPLIFTSKNMKKVVVKEKIHSDHDTLFFTGYHGIFSLQGAVEDLKILSEIGLGFRSSQGFGAVEVV
>CP024101.1|AWC32901.1|2162195_2163944_-|type-I-B-CRISPR-associated-protein-Cas8b1/Cst1
MIFVTEEIPLAAEEWTITQGLVGYYRILKHAGIKVKTTDEGILFNPEHLRNFEHYYFDYFLDRFSVAKRDEQNIRRTFLKFRAESELKGSKEAKRRMQAAKKSLDETIKKTAIEKVEKKFEDSSHKKRLVEYATLIRNQKTYNPEMDKWIEEYIQALSEPNINEKLTLNYFKATVLGTYFGQVSFLNVSHTSKDIEEQKEIFRKDYIESLLADLELQQMIARAEDSDEIMKYLEISTHEMAKKLKTKFKKMTIQQIKDYVSNEINCCSFFDSQWAFEQYSELHFSPLSMSASKALNFYWNANGTLTIPVSKLAKLILFCAPAGASIIGGKSLFVQREGRFEQVIYANETYEIGRSKDKAFDEIIFDLIAEQKTKAQHTGDSYLILEYSSEYNAKKTDLQYLHLTPGLCSFFKEKNCTKWFSRLNYVLRNQIVHAFLRYQDSKHIIHNALRKKLQEQKLANEIVYACLLRCYYLFFVKGEGKMNTDLKTSKNRIWSAFYEGVAIKNIVEEGKIRSVAYRLLNAIRSNDKKMFLDTVMRLYMSVEKPLPRVFMNILDESNIDFASIGDSFIAGLISKEEEKINE
>CP024101.1|AWC32900.1|2161309_2162203_-|type-I-B-CRISPR-associated-protein-Cas7/Cst2/DevR
MNNKALTLTIIFKASSLNYGEGTANISELKKFHRGNGEVYSFASRQAIRYDIVRLGNQLFNWNLNVVDKSKGVVQFKDDCTIADSVEMDLFGYMKTKAKAGADKRSAVVRLSHAVSLEPYRSDLEFLNNMGLASRIQENPNLATLEQHESLYTYTLTVDLSKVGVDGEIELPVDEKVNRVKQLIELTKYLNREIRGRQENLSPLFVIGGVYPIANPFFLGRISLQNFDSNLLNVKSLASVLGQTVLGHDVRTCTKIGLVDGVFANEEEISQLTDTVSVEEFYQHVMNEVEQFYKVLV
>CP024101.1|AWC32899.1|2160614_2161313_-|type-I-B-CRISPR-associated-protein-Cas5
MKVIRLKLFQETACYTKPFANKVAETYPLPPYSTVKGMIHQLLQANELLPLRFSIQGNYETKLIDYRKSYMVKKKSVSMPIIFDGLAMEVPEQEHMTSMPLYTHMLFNVELVIHIAGEEALLERIYQHFIELNQHLSLGRHEDLVRVDEVTFVELEECDEWYSKYAIYAPKYYYKTDFPAGIPYRLNWTYKIVNGIREWNKIPSLYVNGNNPHFEDGKLFHTDGEHIVLWNE
>CP024101.1|AWC32898.1|2158335_2160561_-|CRISPR-associated-helicase/endonuclease-Cas3
MFLAKSNPKETLREHTDELRSRYKKLKESYGHYFDERIWELLLYAVDYHDAGKAHEPFQLKIRRALGEGVKLQRNLEVPHNYLSPFLLPESIFSLPKKDYQALIQAIAYHHERDIEPNDTLIRQALWQDLWPKMDEINKHLNINGANLEKKFNRILAQLHRRIKYSDNKENYVDYVILKGLLHRIDHAASAHVDIEIDAKQDFKKLVDAGFQKIAEKKRVPVEEVYRDLQKHAHKHRKENLIIVAQTGMGKTEAALLWASDSKTFFTLPLRVSINALFHRVYEKMNYKNVGLLHSSSMDYLTENLEEDWEVIAEQSKHFSSKLLFTTIDQILKFPFLYRGYEKELATMAYSSVVIDEIQAYDPKIAAVLVRALEMIYLVGGKFLLMTATLPSIYLETLNESEIISSDGFRVDTFIDDCVIRHCIEVVEKPIVDDFEKIVDLGKQKKILVIANTVKRAMELYNKIKEHSDNVHVFHSMYIQKHRTLIEKELLLFDENKEATGIWITTQLVEASIDIDFDVLFTEAATLDSLCQRFGRCYRRRQYMMHQEPNVFIYAVDMSGVKYVYDEEILMKSLDILRPFNRKILLESQKIELVSQLYSRENLKGTEFLKTFDDALEQLQTFEDYTLSSKDAQKLLRDIQAELVIPRNIYDENEHLFEQLKIVNKEERARLYREIAKLTVSLRKNRVRGKTSRIPHERIQSNGKKYNLLPWIKILELQYDFDEKSKTGTGVLLDQELDNFI
>CP024101.1|AWC32897.1|2157775_2158273_-|CRISPR-associated-protein-Cas4
MKGTYIHYYFVCHRKLWLFVHQIQLEIESERVIEGKILHDSTYQRMEHRELDIDGVGKIDGIEGEYIREIKLTSKMQEADRWQLLYYLAELKKRGVTKKGLLSYKKEKRTEEVHLTEANQQELENIQKRIQAIILSDKPPKLEKKKYCSSCAYYDFCFSGEGEGC
>CP024101.1|AWC32896.1|2156798_2157782_-|type-I-B-CRISPR-associated-endonuclease-Cas1
MLRDFYIFSNGRMKRKDNTFYFINEEEKKRSLPIAQIDNLYVFGQVDFNTKLLNFLNQHQIHVHFFNYYGFYSGSFTPRESNLSGFTLVQQSAHYLDTDKRLILAKAFVSSAVFHMLRTLRGYKQITAEMLGEIIRLTPQISQASTIQQLMGVEGLIRQEYYSMFNYILPPEFSFYKRSKKPPQDEINALISFGNSLCYTTVLSEIYKTQLNPTISYLHEPSSKRYSLSLDIAEIFKPLIVDPLIFTLVNKKMISLKHFESLDEMVLLNEQGRKKFLQAWEGKLGQTVMHRRLKRKVSYKYFIRLECYKLIKHMIGDELYKPLKAWW
>CP024101.1|AWC32895.1|2156530_2156794_-|CRISPR-associated-endonuclease-Cas2
MFVIITYDIGEKRVGKACKKLKQYLTWTQNSVFEGELSRTQLLKCLAELDQIIISSEDSIYLYEVNNPRNIKKKVFGIEKSYEELFL
>CP024101.1|AWC32894.1|2154724_2155108_-|VOC-family-protein
MMIHKVGQIMLYVKDQDEAVQFWTEKMGFHLIAEEDNGKGFRWIEIAPTKEAETSIVLHNKEFIAKMQPELNLNTPSMLFFTENIEQLHKQLVDKKVEVGEIVNMPTGKAFNFADDEGNYFAIMEKS
>CP024101.1|AWC32904.1|2166675_2167002_-|antibiotic-biosynthesis-monooxygenase
MFIETKTLTVKEGTSDLVVQRFSGEGMIEKFEGFIDLNVLVKKVRRGDEEVVIMIRWESEEAWKNWETSEEHLAGHRANRGKPKPEHILNAEHAVYYVKATKSAYQQS
>CP024101.1|AWC32905.1|2167343_2167688_+|hypothetical-protein
MRKAILIFIVAIFIIGIVHIAVTPINYKSYTIEALWFASAGCTLIFLAFLNYVLISIKQRQTRFFVICHTANILCTILVAFILTIAFAPHIILLFVLLVSKTLLVIGFQFSSKS
>CP024101.1|AWC32906.1|2167812_2168142_-|hypothetical-protein
MSFNQVLKVIDENIGKRFVLGIDGLSRLGRTTLVKQLEQKLKQKGASFYIFHIDDHIVERNKRYHTKFEEWYEYYYLQWDIEWLRYHFFQQLKWKEQFRFLGASPKTPS
>CP024101.1|AWC32907.1|2168120_2168687_-|N-acetyltransferase
MGFPTLETERFILRELTLLDAENMFHYFQKESVMRYFGMDAIRNMEQVKRMIQMFSKKYKEGSQVRWGIELKGTNRFIGTCGFHFINLNHKRAEIGYELDDAYWGKGYATEAVQTIVTYAFQTMKLIRIGAVVYKENEASHKLLKKIGFQKEGLLRKYMIQNNVEYDTIIYSLLAEDWEKKSCLSIKY
>CP024101.1|AWC32908.1|2169056_2169302_+|DNA-alkylation-repair-protein
MEPMLCPNCKTNRTRFNILEQQVKPVKLNPQTGQIEEEYTNETLNVFHMSYQGPTYRVQCAVCGLVENPEQFIKPAQNKLF
>CP024101.1|AWC32909.1|2169539_2170076_-|DUF2812-domain-containing-protein
MEIKKTFKFFTAWNLEKEEAYLREMHQKGWAFQKYHFMYTFKKTEPKDVVYKADFNFDFQKSLKNQREYLEIYEMSGWKYVSSFTKWHYFCKEVVDKNELPDIYSETETRIEKLKDLLNHLVIIFLSILPAISISCLESPANKLSIIIKGFIGFVVCIHMYMFVRLILKIRKLKKEII
>CP024101.1|AWC32910.1|2170077_2170401_-|PadR-family-transcriptional-regulator
MDEKAKKYIPLTEATYYILLSFAKRIHGYGVMQMVEEMTDGEVKLGPGTLYGNITKLLTEKLIVEVDSTDRKKCYMLTPFGREVLELEFKRLQRSVQNGKQILREKM
>CP024101.1|AWC32911.1|2171002_2171986_-|DUF4003-domain-containing-protein
MITLQQKLEQYTSIYKQLKRELKWKTSDSRTGMMIAAMYTSSDKPFDLERFLQISHYIKSQVGMFSYLKSYHRFVVAATLDIHFTHYKTAFQQFLDLYERFIASGFSRSIFTYLAAATLLPDDREQYEARIQRSVKVYKRMKEDHFFLTSTSDYPLAVLLAGQQEEIGPLMDRVERLYQKLATSGLRKGNDLQFLSHILSLKKDIREDMLVARCTNIWNLFKQEKVKVKQMHYPAIGLLALLEDGEKEVSSIRTFMEKLQGDKLFRWQTDTNILIAIQLFVSQKGMEHQAANTGLQTMIEILIQAQQAAFIAAISASTAATSASSNS
>CP024101.1|AWC32912.1|2172066_2172534_-|NUDIX-domain-containing-protein
MDLTFQVNQSCFNYRVGAICKHDNKILMLQNEGEDFWYVPGGRVQLLETSEVAIKRELKEELGVDVAVKRPLWIVENFFTYDSRQFHEISLYYEVNLLTLPVKGADSFLFEEEGRKYKFQWIPLEQICEYNVKPEFLKDKMTQLPLHVEHIVKSQ
>CP024101.1|AWC32913.1|2172892_2173138_-|hypothetical-protein
MKGIFWLNLYMSLHVRMGETLYYGLGVRITNSHSEEKVIRHSGLTPNSHAKMFFIPKTDWGGVILTNKNHIFEEEALPQLK

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Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity
CP024101_2	2.2\|2037836\|30\|CP024101\|PILER-CR,CRISPRCasFinder,CRT	2037836-2037865	30	MN693012	Marine virus AFVG_117M3, complete genome	54240-54269	5	0.833
CP024101_2	2.1\|2037770\|30\|CP024101\|PILER-CR,CRISPRCasFinder,CRT	2037770-2037799	30	NZ_CP051687	Duganella sp. GN2-R2 plasmid unnamed2, complete sequence	5370-5399	7	0.767
CP024101_2	2.2\|2037836\|30\|CP024101\|PILER-CR,CRISPRCasFinder,CRT	2037836-2037865	30	CP002966	Emticicia oligotrophica DSM 17448 plasmid pEMTOL05, complete sequence	19362-19391	7	0.767
CP024101_2	2.3\|2037902\|30\|CP024101\|PILER-CR,CRISPRCasFinder,CRT	2037902-2037931	30	AP014136	Uncultured Mediterranean phage uvMED isolate uvMED-GF-C47-MedDCM-OCT-S34-C10, * SEQUENCING IN PROGRESS *	6621-6650	7	0.767
CP024101_2	2.5\|2038034\|30\|CP024101\|PILER-CR,CRISPRCasFinder,CRT	2038034-2038063	30	NZ_CP016619	Microvirga ossetica strain V5/3m plasmid unnamed2, complete sequence	891211-891240	7	0.767
CP024101_2	2.3\|2037902\|30\|CP024101\|PILER-CR,CRISPRCasFinder,CRT	2037902-2037931	30	NZ_CP042421	Leuconostoc lactis strain CBA3622 plasmid unnamed1, complete sequence	41642-41671	8	0.733
CP024101_2	2.2\|2037836\|30\|CP024101\|PILER-CR,CRISPRCasFinder,CRT	2037836-2037865	30	NZ_CP053818	Vibrio cholerae strain SA7G plasmid pSA7G1, complete sequence	3264-3293	9	0.7
CP024101_2	2.3\|2037902\|30\|CP024101\|PILER-CR,CRISPRCasFinder,CRT	2037902-2037931	30	NZ_CP016596	Bacillus cereus strain K8 plasmid pBCM301, complete sequence	119389-119418	9	0.7
CP024101_2	2.3\|2037902\|30\|CP024101\|PILER-CR,CRISPRCasFinder,CRT	2037902-2037931	30	NZ_CP007513	Bacillus bombysepticus plasmid pBb, complete genome	512619-512648	9	0.7
CP024101_2	2.3\|2037902\|30\|CP024101\|PILER-CR,CRISPRCasFinder,CRT	2037902-2037931	30	NZ_CP010112	Bacillus thuringiensis serovar indiana strain HD521 plasmid pBTHD521-6, complete sequence	11573-11602	9	0.7
CP024101_2	2.3\|2037902\|30\|CP024101\|PILER-CR,CRISPRCasFinder,CRT	2037902-2037931	30	MH616843	Inoviridae sp. isolate ctbd25, complete genome	1309-1338	9	0.7

1. spacer 2.2|2037836|30|CP024101|PILER-CR,CRISPRCasFinder,CRT matches to MN693012 (Marine virus AFVG_117M3, complete genome) position: , mismatch: 5, identity: 0.833

aaagaccctacacataatgaatggaaatat	CRISPR spacer
acaggtcctacacataatcaatggaaagat	Protospacer
* **..************ ******** **

2. spacer 2.1|2037770|30|CP024101|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP051687 (Duganella sp. GN2-R2 plasmid unnamed2, complete sequence) position: , mismatch: 7, identity: 0.767

ccacctt--tgaaacgcgtctggatcaacgag	CRISPR spacer
--gacttggcgaaacgcgtctggatccacgat	Protospacer
  . ***  .**************** ****

3. spacer 2.2|2037836|30|CP024101|PILER-CR,CRISPRCasFinder,CRT matches to CP002966 (Emticicia oligotrophica DSM 17448 plasmid pEMTOL05, complete sequence) position: , mismatch: 7, identity: 0.767

aaagaccctacacataatgaatggaaatat	CRISPR spacer
agggattgaacaaataatgaatggaaatat	Protospacer
*..**..  *** *****************

4. spacer 2.3|2037902|30|CP024101|PILER-CR,CRISPRCasFinder,CRT matches to AP014136 (Uncultured Mediterranean phage uvMED isolate uvMED-GF-C47-MedDCM-OCT-S34-C10, *** SEQUENCING IN PROGRESS ***) position: , mismatch: 7, identity: 0.767

tacaaaaacaaatgaaattatcgatagccg	CRISPR spacer
tacaaaagcaaatgaaattgtcgcttgtgc	Protospacer
*******.***********.*** * *.

5. spacer 2.5|2038034|30|CP024101|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP016619 (Microvirga ossetica strain V5/3m plasmid unnamed2, complete sequence) position: , mismatch: 7, identity: 0.767

tagccaaatttcaatgtcatcggaaataat	CRISPR spacer
aagccaaatttccatgtcatcggtgaggtt	Protospacer
 *********** ********** .* . *

6. spacer 2.3|2037902|30|CP024101|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP042421 (Leuconostoc lactis strain CBA3622 plasmid unnamed1, complete sequence) position: , mismatch: 8, identity: 0.733

tacaaaaacaaatgaaattatcgatagccg	CRISPR spacer
caaaaaaacaaatgaaaaaatcgataagtc	Protospacer
.* **************  *******. .

7. spacer 2.2|2037836|30|CP024101|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP053818 (Vibrio cholerae strain SA7G plasmid pSA7G1, complete sequence) position: , mismatch: 9, identity: 0.7

aaagaccctacacataatgaatggaaatat	CRISPR spacer
cgcgtaccgacacataatgaatggaaagtg	Protospacer
 . *  ** ******************

8. spacer 2.3|2037902|30|CP024101|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP016596 (Bacillus cereus strain K8 plasmid pBCM301, complete sequence) position: , mismatch: 9, identity: 0.7

tacaaaaacaaatgaaattatcgatagccg	CRISPR spacer
agaaaaaacacatgaaattatccataaagt	Protospacer
 . ******* *********** ***.

9. spacer 2.3|2037902|30|CP024101|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP007513 (Bacillus bombysepticus plasmid pBb, complete genome) position: , mismatch: 9, identity: 0.7

tacaaaaacaaatgaaattatcgatagccg	CRISPR spacer
agaaaaaacacatgaaattatccataaagt	Protospacer
 . ******* *********** ***.

10. spacer 2.3|2037902|30|CP024101|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP010112 (Bacillus thuringiensis serovar indiana strain HD521 plasmid pBTHD521-6, complete sequence) position: , mismatch: 9, identity: 0.7

tacaaaaacaaatgaaattatcgatagccg	CRISPR spacer
agaaaaaacacatgaaattatccataaagt	Protospacer
 . ******* *********** ***.

11. spacer 2.3|2037902|30|CP024101|PILER-CR,CRISPRCasFinder,CRT matches to MH616843 (Inoviridae sp. isolate ctbd25, complete genome) position: , mismatch: 9, identity: 0.7

tacaaaaacaaatgaaattatcgatagccg	CRISPR spacer
agcaaaaacaaatgaaagtatcggtcttaa	Protospacer
 .*************** *****.*  . .

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

269590 : 277568

uncultured_virus(33.33%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AWC31288.1\|269590_269875_+	co-chaperone GroES	A0A221S3C8	uncultured_virus	5.8e-20	52.7
AWC31289.1\|269909_271538_+	molecular chaperone GroEL	A0A219YK78	uncultured_virus	1.5e-157	56.9
AWC31290.1\|271964_273512_+	glutamine-hydrolyzing GMP synthase	A0A1V0SH76	Hokovirus	2.2e-20	30.6
AWC31291.1\|273885_275211_+	NCS2 family permease	A0A0R6PHV4	Moraxella_phage	4.4e-46	31.8
AWC31292.1\|275353_276055_+	DNA-binding response regulator	W8CYM9	Bacillus_phage	8.6e-41	38.7
AWC34824.1\|276080_277568_+	sensor histidine kinase	W8CYF6	Bacillus_phage	2.8e-33	30.4

DBSCAN-SWA_2

310544 : 318916

Synechococcus_phage(33.33%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AWC31303.1\|310544_311846_+	adenylosuccinate lyase	A0A1B3B081	Gordonia_phage	2.0e-19	31.6
AWC31304.1\|311940_312660_+	phosphoribosylaminoimidazolesuccinocarboxamide synthase	G8EYA2	Synechococcus_phage	9.8e-48	43.9
AWC31305.1\|312652_312907_+	phosphoribosylformylglycinamidine synthase subunit PurS	NA	NA	NA	NA
AWC31306.1\|312903_313587_+	phosphoribosylformylglycinamidine synthase subunit PurQ	NA	NA	NA	NA
AWC31307.1\|313570_315790_+	phosphoribosylformylglycinamidine synthase II	A6N228	Microbacterium_phage	2.0e-160	41.2
AWC31308.1\|315774_317190_+	amidophosphoribosyltransferase	A0A0M3SGR2	Mollivirus	9.5e-55	35.4
AWC31309.1\|317292_318336_+	phosphoribosylformylglycinamidine cyclo-ligase	Q58MH8	Prochlorococcus_phage	2.7e-70	48.0
AWC31310.1\|318328_318916_+	phosphoribosylglycinamide formyltransferase	M4QRX9	Synechococcus_phage	1.6e-24	34.2

DBSCAN-SWA_3

975821 : 1028274

Bacillus_phage(43.48%)

capsid,tail,portal,terminase,holin,protease,head

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AWC31825.1\|975821_976640_+\|protease	CPBP family intramembrane metalloprotease	NA	NA	NA	NA
AWC31826.1\|976676_977036_+	hypothetical protein	NA	NA	NA	NA
AWC34841.1\|977085_977385_+	hypothetical protein	NA	NA	NA	NA
AWC31827.1\|977567_978830_+	peptidase M48	NA	NA	NA	NA
AWC31828.1\|978967_980689_+	methyl-accepting chemotaxis protein	A0A2H4J162	uncultured_Caudovirales_phage	6.2e-16	30.6
AWC34842.1\|980911_981799_+	EamA/RhaT family transporter	NA	NA	NA	NA
AWC31829.1\|982271_982931_+	S-layer homology domain-containing protein	NA	NA	NA	NA
AWC31830.1\|983248_983893_+	S-layer protein	NA	NA	NA	NA
AWC31831.1\|985720_986998_+	isocitrate lyase	NA	NA	NA	NA
AWC31832.1\|987328_987532_+	cold-shock protein	Q9AZD3	Lactococcus_phage	1.0e-15	61.3
AWC31833.1\|988169_989603_-	recombinase family protein	A0A2P1JU08	Anoxybacillus_phage	7.0e-114	47.3
AWC31834.1\|989634_990072_-	ImmA/IrrE family metallo-endopeptidase	D6R412	Bacillus_phage	3.9e-31	50.4
AWC31835.1\|990094_990502_-	XRE family transcriptional regulator	A0A0M4QX29	Bacillus_phage	5.0e-25	47.1
AWC31836.1\|990779_990980_+	XRE family transcriptional regulator	A0A0C5AJQ3	Paenibacillus_phage	3.2e-09	50.9
AWC31837.1\|991326_991677_+	hypothetical protein	A0A2H4JBP9	uncultured_Caudovirales_phage	7.1e-44	77.4
AWC31838.1\|991766_992084_+	hypothetical protein	A0A2H4J830	uncultured_Caudovirales_phage	2.1e-15	48.8
AWC31839.1\|992031_992232_+	hypothetical protein	NA	NA	NA	NA
AWC31840.1\|992233_992425_+	hypothetical protein	NA	NA	NA	NA
AWC31841.1\|992430_993132_+	DNA-binding protein	A0A2H4JHI2	uncultured_Caudovirales_phage	3.6e-71	58.1
AWC31842.1\|993131_993608_+	DUF669 domain-containing protein	A0A1B2AQ07	Phage_Wrath	1.6e-30	49.4
AWC31843.1\|993712_996067_+	DNA primase	A0A1B2AQ05	Phage_Wrath	0.0e+00	83.2
AWC31844.1\|996332_996761_+	hypothetical protein	A0A2H4JFQ6	uncultured_Caudovirales_phage	3.4e-64	78.7
AWC31845.1\|996763_997303_+	nuclease	A0A0S2SXQ1	Bacillus_phage	8.6e-49	53.1
AWC31846.1\|997304_997589_+	hypothetical protein	A0A0H3V0M3	Geobacillus_virus	3.9e-16	52.3
AWC31847.1\|997669_998014_+	organic solvent tolerance protein OstA	A0A1B1P7N6	Bacillus_phage	2.5e-25	56.6
AWC31848.1\|998097_998298_+	hypothetical protein	A0A1L2JY31	Aeribacillus_phage	6.9e-12	53.0
AWC31849.1\|998400_999204_+	hypothetical protein	NA	NA	NA	NA
AWC31850.1\|999227_1000412_+	transcriptional regulator	A0A0A7RTT7	Clostridium_phage	1.6e-34	27.9
AWC31851.1\|1000513_1000909_+	DUF1492 domain-containing protein	A0A2H4JFW6	uncultured_Caudovirales_phage	2.2e-57	86.3
AWC31852.1\|1001086_1002001_+	hypothetical protein	C9E2Q0	Enterococcus_phage	5.6e-24	35.9
AWC31853.1\|1002062_1002593_-	pilus assembly protein HicB	A0A090DBV2	Clostridium_phage	7.5e-29	51.1
AWC34843.1\|1002688_1002859_-	type II toxin-antitoxin system HicA family toxin	NA	NA	NA	NA
AWC31854.1\|1003093_1003333_+	hydrolase	NA	NA	NA	NA
AWC31855.1\|1003346_1003643_+	HNH endonuclease	A0A0S2SXR6	Bacillus_phage	1.7e-27	64.0
AWC31856.1\|1003629_1003866_+	hypothetical protein	A0A0S2GM40	Bacillus_phage	1.4e-24	82.9
AWC31857.1\|1003980_1004367_+	hypothetical protein	A0A0S2SXN4	Bacillus_phage	4.4e-55	87.8
AWC31858.1\|1004363_1006082_+\|terminase	terminase large subunit	A0A0S2SXJ7	Bacillus_phage	2.0e-301	86.5
AWC31859.1\|1006104_1007274_+\|portal	phage portal protein	A0A1S5SFK2	Streptococcus_phage	2.5e-133	58.1
AWC31860.1\|1007266_1007839_+\|head,protease	HK97 family phage prohead protease	A0A0S2SXJ4	Bacillus_phage	1.0e-71	69.5
AWC31861.1\|1007831_1009004_+\|capsid	phage major capsid protein	A0A0S2SXJ6	Bacillus_phage	1.1e-154	74.6
AWC31862.1\|1009021_1009201_+	hypothetical protein	A0A0S2SXV2	Bacillus_phage	1.2e-12	64.3
AWC31863.1\|1009193_1009487_+	hypothetical protein	A0A0S2SXS0	Bacillus_phage	1.5e-23	57.3
AWC31864.1\|1009473_1009818_+	hypothetical protein	NA	NA	NA	NA
AWC31865.1\|1009810_1010167_+	hypothetical protein	D2XR21	Bacillus_phage	7.5e-33	59.0
AWC31866.1\|1010163_1010493_+	hypothetical protein	D2XR22	Bacillus_phage	1.4e-54	96.3
AWC31867.1\|1010493_1011087_+\|tail	phage tail protein	D2XR23	Bacillus_phage	1.5e-94	85.6
AWC31868.1\|1011093_1011450_+	hypothetical protein	A0A2H4JAG8	uncultured_Caudovirales_phage	2.0e-41	70.8
AWC31869.1\|1011680_1013144_+	hypothetical protein	A0A2H4JAI2	uncultured_Caudovirales_phage	1.0e-189	86.8
AWC31870.1\|1013362_1015543_+	hypothetical protein	A0A2H4JC82	uncultured_Caudovirales_phage	2.1e-29	70.4
AWC31871.1\|1015584_1017060_+\|tail	phage tail protein	D2XR27	Bacillus_phage	8.7e-285	95.1
AWC31872.1\|1017056_1021751_+	peptidase S74	D2XR28	Bacillus_phage	0.0e+00	64.9
AWC31873.1\|1021773_1022223_+	hypothetical protein	A0A2H4J6F8	uncultured_Caudovirales_phage	1.4e-76	94.6
AWC31874.1\|1022392_1022629_+	hypothetical protein	A0A2H4J382	uncultured_Caudovirales_phage	1.5e-18	96.2
AWC31875.1\|1022628_1022868_+\|holin	holin	A0A2H4J378	uncultured_Caudovirales_phage	1.7e-33	98.7
AWC31876.1\|1022864_1023929_+	N-acetylmuramoyl-L-alanine amidase	A0A1B1P878	Bacillus_phage	9.3e-196	94.4
AWC31877.1\|1023967_1024510_-	hypothetical protein	NA	NA	NA	NA
AWC31878.1\|1024645_1024891_+	hypothetical protein	NA	NA	NA	NA
AWC31879.1\|1024892_1025741_+	AraC family transcriptional regulator	A0A1B0RXG1	Streptococcus_phage	2.7e-20	38.6
AWC31880.1\|1026045_1026408_-	hypothetical protein	NA	NA	NA	NA
AWC31881.1\|1026469_1026802_-	hypothetical protein	A0A1B2AQ49	Phage_Wrath	4.1e-17	49.1
AWC31882.1\|1026880_1027129_-	hypothetical protein	A0A1B1P7Q5	Bacillus_phage	1.1e-38	97.6
AWC31883.1\|1027125_1027350_-	transcriptional regulator	A0A1B1P7Q3	Bacillus_phage	7.7e-36	100.0
AWC31884.1\|1027443_1028274_-	cytosolic protein	A0A2H4J9W9	uncultured_Caudovirales_phage	3.8e-120	89.1

DBSCAN-SWA_4

1098883 : 1164988

Escherichia_phage(18.18%)

coat,bacteriocin,integrase

attL 1138598:1138625|attR 1167610:1167637

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AWC31953.1\|1098883_1099459_-\|coat	spore coat protein	NA	NA	NA	NA
AWC31954.1\|1099580_1099940_+	DUF1360 domain-containing protein	NA	NA	NA	NA
AWC34847.1\|1100064_1100223_+	cytochrome C oxidase subunit III	NA	NA	NA	NA
AWC34848.1\|1100289_1101225_-	glycosyl transferase family 2	NA	NA	NA	NA
AWC31955.1\|1101247_1102003_-	class I SAM-dependent methyltransferase	NA	NA	NA	NA
AWC31956.1\|1102192_1103038_-	macrocin O-methyltransferase	A0A2R8FFV8	Cedratvirus	5.5e-58	55.2
AWC31957.1\|1103162_1104149_-	hypothetical protein	NA	NA	NA	NA
AWC34849.1\|1104282_1105380_+	beta 1,4 glucosyltransferase	A0A0N9QAD0	Chrysochromulina_ericina_virus	1.5e-10	25.2
AWC31958.1\|1105464_1106151_+	class I SAM-dependent methyltransferase	NA	NA	NA	NA
AWC31959.1\|1106147_1106825_+	streptomycin biosynthesis protein StrF	NA	NA	NA	NA
AWC31960.1\|1106844_1107525_+	streptomycin biosynthesis protein StrF	NA	NA	NA	NA
AWC31961.1\|1107601_1108339_+\|coat	spore coat protein	I7I009	Enterobacteria_phage	2.8e-50	44.7
AWC31962.1\|1108347_1108896_+	dTDP-4-dehydrorhamnose 3,5-epimerase	A0A218MN57	uncultured_virus	1.7e-31	43.2
AWC31963.1\|1108907_1109879_+	dTDP-glucose 4,6-dehydratase	A0A1D7XFE8	Escherichia_phage	8.5e-71	42.9
AWC31964.1\|1109890_1110742_+	dTDP-4-dehydrorhamnose reductase	A0A291LA50	Escherichia_phage	9.5e-42	40.2
AWC31965.1\|1110944_1111445_-	hypothetical protein	NA	NA	NA	NA
AWC31966.1\|1111879_1112353_-\|coat	spore coat protein	NA	NA	NA	NA
AWC31967.1\|1112519_1114574_-	ATP-dependent helicase	S5MMD7	Bacillus_phage	6.2e-79	31.9
AWC31968.1\|1115099_1115618_-	hypothetical protein	NA	NA	NA	NA
AWC31969.1\|1115710_1116442_-	esterase family protein	NA	NA	NA	NA
AWC31970.1\|1116662_1117574_+	EamA/RhaT family transporter	NA	NA	NA	NA
AWC31971.1\|1117794_1118184_-	hypothetical protein	A0A0E3TB73	Enterococcus_phage	6.3e-09	36.6
AWC31972.1\|1118355_1119822_-	sodium/proline symporter PutP	NA	NA	NA	NA
AWC31973.1\|1121654_1123148_+	lactate permease	NA	NA	NA	NA
AWC31974.1\|1123252_1123936_+	DUF2278 domain-containing protein	NA	NA	NA	NA
AWC31975.1\|1123982_1124816_-	hypothetical protein	NA	NA	NA	NA
AWC31976.1\|1124944_1125691_+	zinc ribbon domain-containing protein	NA	NA	NA	NA
AWC31977.1\|1125865_1127200_+	CoA-disulfide reductase	NA	NA	NA	NA
AWC31978.1\|1127328_1127739_+	DUF3908 domain-containing protein	NA	NA	NA	NA
AWC31979.1\|1127952_1129497_-	NADH oxidase	NA	NA	NA	NA
AWC31980.1\|1129557_1131510_-\|bacteriocin	bacteriocin biosynthesis protein SagD	NA	NA	NA	NA
AWC31981.1\|1131503_1133429_-\|bacteriocin	putative thiazole-containing bacteriocin maturation protein	NA	NA	NA	NA
AWC31982.1\|1133587_1134832_-	dihydrolipoyllysine-residue succinyltransferase	NA	NA	NA	NA
AWC31983.1\|1134952_1137829_-	2-oxoglutarate dehydrogenase subunit E1	NA	NA	NA	NA
1138598:1138625	attL	NA	NA	NA	NA
AWC31984.1\|1138796_1138979_+	DUF3976 domain-containing protein	NA	NA	NA	NA
AWC31985.1\|1139040_1139925_-	LysR family transcriptional regulator	NA	NA	NA	NA
AWC31986.1\|1140111_1141038_+	2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase	NA	NA	NA	NA
AWC31987.1\|1141107_1142631_+	5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase	NA	NA	NA	NA
AWC31988.1\|1142684_1144157_+	4-hydroxyphenylacetate 3-monooxygenase, oxygenase component	NA	NA	NA	NA
AWC31989.1\|1144176_1145160_+	3,4-dihydroxyphenylacetate 2,3-dioxygenase	NA	NA	NA	NA
AWC31990.1\|1145217_1146084_+	cytidyltransferase	NA	NA	NA	NA
AWC31991.1\|1146144_1146474_+	DUF485 domain-containing protein	NA	NA	NA	NA
AWC31992.1\|1146463_1148014_+	cation acetate symporter	NA	NA	NA	NA
AWC31993.1\|1148034_1148826_+	4-hydroxyphenylacetate isomerase	NA	NA	NA	NA
AWC31994.1\|1148822_1149581_+	2-hydroxyhepta-2,4-diene-1,7-dioate isomerase	NA	NA	NA	NA
AWC31995.1\|1149653_1150052_+	5-carboxymethyl-2-hydroxymuconate isomerase	NA	NA	NA	NA
AWC31996.1\|1150370_1151483_+	tetratricopeptide repeat-containing protein	A0A2H4J8G3	uncultured_Caudovirales_phage	2.4e-93	49.1
AWC31997.1\|1152133_1152502_+	NUDIX domain-containing protein	NA	NA	NA	NA
AWC31998.1\|1153325_1153532_+	hypothetical protein	NA	NA	NA	NA
AWC31999.1\|1154182_1154500_+	DUF3884 domain-containing protein	NA	NA	NA	NA
AWC32000.1\|1156557_1156965_+	hypothetical protein	NA	NA	NA	NA
AWC32001.1\|1159263_1160184_-	gfo/Idh/MocA family oxidoreductase	NA	NA	NA	NA
AWC32002.1\|1160265_1160643_-	hypothetical protein	NA	NA	NA	NA
AWC32003.1\|1160659_1160920_-	hypothetical protein	NA	NA	NA	NA
AWC32004.1\|1161055_1161310_+	hypothetical protein	NA	NA	NA	NA
AWC32005.1\|1161816_1162470_+	hypothetical protein	NA	NA	NA	NA
AWC32006.1\|1162667_1162943_+	hypothetical protein	NA	NA	NA	NA
AWC32007.1\|1163260_1164355_-	tetratricopeptide repeat-containing protein	A0A2H4J8G3	uncultured_Caudovirales_phage	1.2e-100	51.8
AWC32008.1\|1164616_1164988_+\|integrase	site-specific integrase	A0A1B0T6A8	Bacillus_phage	2.1e-25	54.4
1167610:1167637	attR	NA	NA	NA	NA

DBSCAN-SWA_5

1978402 : 1986535

Bacillus_phage(50.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AWC34884.1\|1978402_1979320_+	ABC transporter ATP-binding protein	A0A2H4PQG7	Staphylococcus_phage	7.6e-45	43.3
AWC32742.1\|1979316_1980069_+	ABC transporter permease	NA	NA	NA	NA
AWC32743.1\|1980081_1980813_+	multidrug ABC transporter permease	NA	NA	NA	NA
AWC32744.1\|1981245_1981959_+	DNA-binding response regulator	W8CYM9	Bacillus_phage	3.7e-39	41.5
AWC32745.1\|1981959_1983366_+	two-component sensor histidine kinase	A0A1V0SGX0	Hokovirus	3.2e-10	24.4
AWC32746.1\|1983722_1984061_-	DUF4064 domain-containing protein	A0A0S2MVJ2	Bacillus_phage	2.1e-40	80.4
AWC32747.1\|1984077_1984581_-	DUF4352 domain-containing protein	NA	NA	NA	NA
AWC32748.1\|1985243_1985522_+	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	A0A2I7SC16	Paenibacillus_phage	5.1e-13	61.9
AWC32749.1\|1985740_1986535_+	peptigoglycan-binding protein LysM	A0A141HRV8	Bacillus_phage	6.3e-32	40.7

DBSCAN-SWA_6

2278076 : 2344241

Bacillus_phage(42.86%)

holin,bacteriocin,transposase

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AWC33009.1\|2278076_2278694_+\|transposase	transposase	NA	NA	NA	NA
AWC34903.1\|2279192_2279936_-	hypothetical protein	A0A127AXI2	Bacillus_phage	1.7e-18	44.5
AWC33010.1\|2280396_2281626_-	MFS transporter	NA	NA	NA	NA
AWC33011.1\|2282371_2282608_-	hypothetical protein	NA	NA	NA	NA
AWC33012.1\|2282638_2283580_-\|transposase	transposase	NA	NA	NA	NA
AWC33013.1\|2285745_2286171_+	hypothetical protein	NA	NA	NA	NA
AWC33014.1\|2286145_2286439_+	hypothetical protein	NA	NA	NA	NA
AWC33015.1\|2286720_2287862_-\|transposase	IS3 family transposase	A0A1P8CWQ3	Bacillus_phage	5.1e-99	66.4
AWC33016.1\|2287897_2288515_-\|bacteriocin	bacteriocin ABC transporter ATP-binding protein	G9BWD6	Planktothrix_phage	2.9e-16	34.3
AWC33017.1\|2288519_2290688_-\|bacteriocin	bacteriocin-associated protein	NA	NA	NA	NA
AWC33018.1\|2290811_2291000_-	hypothetical protein	NA	NA	NA	NA
AWC33019.1\|2290984_2291254_-	hypothetical protein	NA	NA	NA	NA
AWC33020.1\|2292350_2292599_-	DUF4176 domain-containing protein	NA	NA	NA	NA
AWC34904.1\|2292599_2292761_-	hypothetical protein	NA	NA	NA	NA
AWC33021.1\|2292799_2292991_-	hypothetical protein	NA	NA	NA	NA
AWC33022.1\|2294680_2294956_-	TIGR04197 family type VII secretion effector	NA	NA	NA	NA
AWC33023.1\|2298247_2298994_-	ABC transporter ATP-binding protein	G9BWD6	Planktothrix_phage	5.0e-31	37.1
AWC33024.1\|2299363_2299687_-	hypothetical protein	NA	NA	NA	NA
AWC33025.1\|2299694_2300219_-	hypothetical protein	NA	NA	NA	NA
AWC33026.1\|2302351_2302735_-	hypothetical protein	NA	NA	NA	NA
AWC33027.1\|2302953_2303274_-	hypothetical protein	NA	NA	NA	NA
AWC33028.1\|2305123_2306104_+	ketoacyl-ACP synthase III	NA	NA	NA	NA
AWC33029.1\|2306166_2306415_-	hypothetical protein	A0A288WFY7	Bacillus_phage	3.9e-12	46.2
AWC33030.1\|2306687_2307737_-	oxidoreductase	NA	NA	NA	NA
AWC33031.1\|2308219_2308618_+	DUF2871 domain-containing protein	NA	NA	NA	NA
AWC33032.1\|2309516_2309777_-	DUF2642 domain-containing protein	NA	NA	NA	NA
AWC33033.1\|2309916_2310579_-	bacillithiol biosynthesis deacetylase BshB2	NA	NA	NA	NA
AWC33034.1\|2310594_2310945_-	DUF1806 domain-containing protein	NA	NA	NA	NA
AWC33035.1\|2311186_2312512_+	hypothetical protein	NA	NA	NA	NA
AWC33036.1\|2312851_2313379_-	N-acetyltransferase	A0A1P8CWJ6	Bacillus_phage	4.8e-60	66.3
AWC33037.1\|2313385_2315287_-	mannonate oxidoreductase	F2Y0V3	Organic_Lake_phycodnavirus	2.0e-15	25.4
AWC33038.1\|2316760_2318548_+	oligoendopeptidase F	NA	NA	NA	NA
AWC33039.1\|2318733_2319966_-	MFS transporter	NA	NA	NA	NA
AWC33040.1\|2320069_2320933_+	LysR family transcriptional regulator	NA	NA	NA	NA
AWC33041.1\|2321100_2322159_+	acyltransferase	NA	NA	NA	NA
AWC33042.1\|2322203_2323148_-	glycerophosphodiester phosphodiesterase	A0A076G4Q2	Staphylococcus_phage	2.4e-38	35.0
AWC33043.1\|2323622_2324165_-	N-acetyltransferase	NA	NA	NA	NA
AWC33044.1\|2324415_2324829_-	hypothetical protein	NA	NA	NA	NA
AWC33045.1\|2325407_2326256_-	chemotaxis protein	A0A2H4J162	uncultured_Caudovirales_phage	1.4e-08	47.9
AWC33046.1\|2327338_2328151_-	hypothetical protein	NA	NA	NA	NA
AWC33047.1\|2328632_2330012_+	chitin-binding protein	A0A0K1Y848	Apis_mellifera_filamentous_virus	1.4e-21	35.7
AWC33048.1\|2330165_2331836_-	peptidase M4 family protein	NA	NA	NA	NA
AWC33049.1\|2332571_2334047_+	SELO family protein	A0A075BSJ0	Microcystis_phage	1.2e-47	30.9
AWC33050.1\|2334162_2335548_-	2-hydroxy-acid oxidase	NA	NA	NA	NA
AWC33051.1\|2335972_2336650_-	methyltransferase	G3MA03	Bacillus_virus	8.1e-20	43.3
AWC33052.1\|2337465_2338377_-	hypothetical protein	NA	NA	NA	NA
AWC33053.1\|2338782_2339130_+	hypothetical protein	NA	NA	NA	NA
AWC33054.1\|2339271_2340339_+	hypothetical protein	NA	NA	NA	NA
AWC33055.1\|2341588_2341921_-	hypothetical protein	NA	NA	NA	NA
AWC33056.1\|2342092_2342296_+	XRE family transcriptional regulator	NA	NA	NA	NA
AWC33057.1\|2342375_2342960_+	hypothetical protein	NA	NA	NA	NA
AWC33058.1\|2343000_2343801_-	N-acetylmuramoyl-L-alanine amidase	D2XR33	Bacillus_phage	3.0e-122	74.9
AWC33059.1\|2343800_2344241_-\|holin	holin	A0A1B1P7S2	Bacillus_phage	8.3e-66	86.3

DBSCAN-SWA_7

2348769 : 2392476

Bacillus_phage(46.67%)

capsid,tail,integrase,portal

attL 2339816:2339875|attR 2392566:2393087

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
2339816:2339875	attL	NA	NA	NA	NA
AWC33061.1\|2348769_2350272_-\|tail	phage tail protein	A0A1B1P7W7	Bacillus_phage	7.2e-202	72.4
AWC33062.1\|2350286_2353940_-\|tail	phage tail tape measure protein	A6M961	Geobacillus_virus	2.4e-57	46.6
AWC33063.1\|2353986_2354304_-	hypothetical protein	NA	NA	NA	NA
AWC33064.1\|2354345_2354720_-	hypothetical protein	NA	NA	NA	NA
AWC33065.1\|2354777_2355308_-\|tail	phage major tail protein, TP901-1 family	NA	NA	NA	NA
AWC33066.1\|2355326_2355734_-	hypothetical protein	NA	NA	NA	NA
AWC33067.1\|2355736_2356096_-	hypothetical protein	NA	NA	NA	NA
AWC33068.1\|2356095_2356470_-	hypothetical protein	NA	NA	NA	NA
AWC33069.1\|2356473_2357280_-	hypothetical protein	NA	NA	NA	NA
AWC33070.1\|2357276_2357633_-	hypothetical protein	A0A142F1M2	Bacillus_phage	2.0e-09	34.5
AWC33071.1\|2357638_2357851_-	hypothetical protein	NA	NA	NA	NA
AWC33072.1\|2357850_2358876_-\|capsid	minor capsid protein E	A0A142F1M0	Bacillus_phage	5.4e-108	60.1
AWC33073.1\|2358939_2359326_-	hypothetical protein	A0A142F1L9	Bacillus_phage	3.2e-45	68.2
AWC33074.1\|2359348_2359900_-	hypothetical protein	NA	NA	NA	NA
AWC33075.1\|2359900_2361442_-\|portal	phage portal protein	A0A142F1L7	Bacillus_phage	8.3e-137	48.4
AWC33076.1\|2361457_2363161_-	hypothetical protein	A0A142F1L6	Bacillus_phage	2.5e-158	50.3
AWC33077.1\|2363160_2363634_-	hypothetical protein	NA	NA	NA	NA
AWC33078.1\|2364702_2365527_-	hypothetical protein	NA	NA	NA	NA
AWC33079.1\|2365922_2366489_-	hypothetical protein	A0A2H4J2L4	uncultured_Caudovirales_phage	9.7e-35	45.8
AWC33080.1\|2366500_2366878_-	hypothetical protein	A0A0N9RZI0	Paenibacillus_phage	2.0e-15	43.1
AWC33081.1\|2366882_2368460_-	DNA cytosine methyltransferase	Q02778	Bacillus_phage	8.3e-108	42.4
AWC33082.1\|2368456_2368687_-	hypothetical protein	NA	NA	NA	NA
AWC33083.1\|2368688_2369327_-	hypothetical protein	NA	NA	NA	NA
AWC33084.1\|2369338_2369572_-	glutaredoxin family protein	A0A1B1P8E0	Bacillus_phage	1.2e-23	64.5
AWC33085.1\|2369568_2370174_-	hypothetical protein	A0A2H4JAZ2	uncultured_Caudovirales_phage	7.7e-38	41.2
AWC33086.1\|2370234_2370861_-	hypothetical protein	J9Q953	Bacillus_phage	3.3e-36	44.9
AWC34905.1\|2370862_2371234_-	hypothetical protein	NA	NA	NA	NA
AWC33087.1\|2371661_2371970_-	transglycosylase	A0A0K2CZP5	Paenibacillus_phage	9.7e-05	35.7
AWC33088.1\|2371969_2372365_-	alpha/beta hydrolase	A0A2H4IZM2	uncultured_Caudovirales_phage	6.1e-44	68.9
AWC33089.1\|2372650_2373178_-	hypothetical protein	A0A0N9ST03	Paenibacillus_phage	3.2e-08	44.6
AWC33090.1\|2373174_2373399_-	hypothetical protein	U5Q038	Bacillus_phage	8.3e-22	70.6
AWC33091.1\|2373398_2374445_-	hypothetical protein	A0A0N9SHN6	Paenibacillus_phage	4.8e-88	58.6
AWC33092.1\|2374458_2374614_-	hypothetical protein	NA	NA	NA	NA
AWC34906.1\|2374606_2376790_-	DNA-directed DNA polymerase	A0A142F1Q9	Bacillus_phage	7.2e-219	54.6
AWC33093.1\|2376969_2378145_-	hypothetical protein	NA	NA	NA	NA
AWC33094.1\|2378137_2378545_-	hypothetical protein	NA	NA	NA	NA
AWC33095.1\|2378571_2379333_-	single-stranded DNA-binding protein	A0A0N9SJW5	Paenibacillus_phage	7.9e-56	49.2
AWC33096.1\|2379839_2380487_-	MarR family transcriptional regulator	NA	NA	NA	NA
AWC33097.1\|2380784_2382008_-	hypothetical protein	A0A288WGM1	Bacillus_phage	9.9e-101	47.8
AWC33098.1\|2382122_2382326_+	XRE family transcriptional regulator	NA	NA	NA	NA
AWC34907.1\|2382389_2382575_+	XRE family transcriptional regulator	NA	NA	NA	NA
AWC33099.1\|2382582_2383599_-	hypothetical protein	A0A2H4J6E6	uncultured_Caudovirales_phage	6.8e-71	43.2
AWC33100.1\|2383625_2384969_-	DNA helicase	A0A2H4IZL9	uncultured_Caudovirales_phage	2.6e-134	55.8
AWC33101.1\|2385065_2385350_-	hypothetical protein	A0A0H3V0M3	Geobacillus_virus	5.4e-10	44.0
AWC33102.1\|2385597_2386014_-	hypothetical protein	A0A0K2CNU6	Brevibacillus_phage	8.5e-12	57.1
AWC33103.1\|2386051_2386591_-	hypothetical protein	A0A1I9S5V4	Bacillus_phage	2.8e-71	76.7
AWC33104.1\|2386636_2386822_-	hypothetical protein	NA	NA	NA	NA
AWC33105.1\|2387188_2387953_-	DNA replication protein	A0A0N7GFF0	Paenibacillus_phage	3.7e-77	56.5
AWC33106.1\|2387952_2388627_-	DUF723 domain-containing protein	NA	NA	NA	NA
AWC34908.1\|2388642_2389029_-	hypothetical protein	A0A2H4J134	uncultured_Caudovirales_phage	3.2e-13	36.2
AWC33107.1\|2389034_2389367_-	hypothetical protein	NA	NA	NA	NA
AWC33108.1\|2389353_2389512_-	Fur-regulated basic protein FbpA	NA	NA	NA	NA
AWC33109.1\|2389593_2389938_-	hypothetical protein	NA	NA	NA	NA
AWC33110.1\|2390367_2390739_-	helix-turn-helix domain-containing protein	NA	NA	NA	NA
AWC33111.1\|2390735_2391137_-	helix-turn-helix domain-containing protein	A0A142F1P0	Bacillus_phage	2.3e-30	58.5
AWC33112.1\|2391345_2392476_+\|integrase	site-specific integrase	A0A2H4JCB7	uncultured_Caudovirales_phage	3.1e-64	36.5
2392566:2393087	attR	NA	NA	NA	NA

DBSCAN-SWA_8

2550547 : 2594896

Bacillus_phage(90.24%)

tail,portal,integrase,holin,terminase,tRNA,head

attL 2591126:2591140|attR 2597652:2597666

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AWC33248.1\|2550547_2551543_+\|tRNA	tryptophan--tRNA ligase	NA	NA	NA	NA
AWC33249.1\|2552206_2552866_+	hypothetical protein	NA	NA	NA	NA
AWC33250.1\|2553254_2553707_+	hypothetical protein	NA	NA	NA	NA
AWC33251.1\|2554921_2555134_+	hypothetical protein	NA	NA	NA	NA
AWC33252.1\|2556195_2556669_-	hypothetical protein	NA	NA	NA	NA
AWC33253.1\|2556801_2557299_+	exosporium protein D	NA	NA	NA	NA
AWC33254.1\|2559453_2560026_+	DUF4352 domain-containing protein	A0A1B1P898	Bacillus_phage	1.5e-54	84.9
AWC33255.1\|2560336_2560777_+	hypothetical protein	NA	NA	NA	NA
AWC33256.1\|2560851_2561673_-	N-acetylmuramoyl-L-alanine amidase	A0A0S2GLD4	Bacillus_phage	5.9e-142	85.3
AWC33257.1\|2561672_2562113_-\|holin	holin	A0A1B1P7S2	Bacillus_phage	8.3e-66	86.3
AWC33258.1\|2562147_2566641_-	hypothetical protein	A0A0M4RER0	Bacillus_phage	1.2e-265	45.7
AWC33259.1\|2566641_2568135_-\|tail	phage tail protein	A0A1B1P7W7	Bacillus_phage	2.4e-229	76.3
AWC33260.1\|2568147_2571075_-\|tail	phage tail tape measure protein	A0A0A7AR36	Bacillus_phage	2.9e-183	55.7
AWC33261.1\|2571080_2571347_-	hypothetical protein	A0A0M4S6X7	Bacillus_phage	8.3e-21	60.2
AWC33262.1\|2571382_2571820_-	hypothetical protein	A0A0M4QX42	Bacillus_phage	3.6e-13	32.7
AWC33263.1\|2571865_2572498_-	hypothetical protein	A0A0M4R5F9	Bacillus_phage	5.7e-76	79.1
AWC33264.1\|2572515_2572896_-	hypothetical protein	A0A0M4RD75	Bacillus_phage	1.8e-56	83.3
AWC33265.1\|2572892_2573309_-	hypothetical protein	A0A0M4S6Z2	Bacillus_phage	2.7e-58	76.8
AWC33266.1\|2573292_2573652_-	hypothetical protein	A0A0M4S6Z9	Bacillus_phage	5.7e-49	81.2
AWC33267.1\|2573633_2573972_-	hypothetical protein	W8CYS9	Bacillus_phage	2.9e-10	39.6
AWC33268.1\|2574008_2574851_-	hypothetical protein	A0A0M5M1L4	Bacillus_phage	5.2e-117	77.7
AWC33269.1\|2574864_2575455_-	DUF4355 domain-containing protein	A0A0M3ULE7	Bacillus_phage	4.6e-19	55.8
AWC33270.1\|2575524_2576550_-\|head	phage head morphogenesis protein	A0A0M5M7Z2	Bacillus_phage	5.1e-159	84.1
AWC33271.1\|2576536_2577892_-\|portal	phage portal protein	A0A0M4S669	Bacillus_phage	3.5e-200	85.6
AWC33272.1\|2577903_2579208_-\|terminase	PBSX family phage terminase large subunit	A0A0M4R5F3	Bacillus_phage	9.5e-251	95.2
AWC33273.1\|2579194_2579626_-\|terminase	terminase small subunit	A0A0M4RU19	Bacillus_phage	1.3e-55	74.8
AWC33274.1\|2580389_2580635_-	DNA-binding protein	A0A290FZJ4	Caldibacillus_phage	5.1e-17	57.5
AWC33275.1\|2581038_2581419_-	ArpU family transcriptional regulator	A0A0M4R397	Bacillus_phage	1.1e-53	84.7
AWC33276.1\|2581423_2581711_-	hypothetical protein	A6M999	Geobacillus_virus	2.9e-35	73.4
AWC34913.1\|2582090_2582342_-	XRE family transcriptional regulator	A0A0S2MV80	Bacillus_phage	1.3e-36	92.7
AWC33277.1\|2582593_2582878_-	hypothetical protein	I7J4K9	Bacillus_phage	4.3e-15	48.9
AWC33278.1\|2582978_2583188_+	hypothetical protein	NA	NA	NA	NA
AWC33279.1\|2583248_2583680_-	hypothetical protein	W8CYU3	Bacillus_phage	2.6e-19	39.9
AWC33280.1\|2583709_2583913_-	hypothetical protein	NA	NA	NA	NA
AWC33281.1\|2583934_2584468_-	hypothetical protein	U5PUK4	Bacillus_phage	8.8e-54	60.0
AWC33282.1\|2584510_2584717_-	hypothetical protein	NA	NA	NA	NA
AWC33283.1\|2584795_2584996_-	hypothetical protein	A0A1P8CWV4	Bacillus_phage	2.6e-06	54.2
AWC34914.1\|2585032_2585605_-	dUTPase	A0A0A7AQI4	Bacillus_phage	1.7e-82	78.1
AWC33284.1\|2585758_2586550_-	hypothetical protein	A0A0M4RU33	Bacillus_phage	3.8e-29	42.1
AWC33285.1\|2586551_2586731_-	hypothetical protein	NA	NA	NA	NA
AWC33286.1\|2586940_2588545_-	hypothetical protein	A0A0M4RD64	Bacillus_phage	1.6e-114	72.0
AWC33287.1\|2588545_2588815_-	hypothetical protein	A0A0M4S680	Bacillus_phage	5.3e-39	83.1
AWC33288.1\|2588828_2589500_-	DUF1071 domain-containing protein	A0A1B1P7F0	Bacillus_phage	8.0e-28	44.2
AWC33289.1\|2589496_2589979_-	ATPase	E5DV79	Deep-sea_thermophilic_phage	8.3e-35	48.1
AWC33290.1\|2590042_2590411_-	XRE family transcriptional regulator	A0A0M5M3T1	Bacillus_phage	2.9e-11	81.0
AWC33291.1\|2590433_2590700_-	hypothetical protein	A0A0M4S677	Bacillus_phage	2.3e-23	80.9
AWC33292.1\|2591019_2591208_-	hypothetical protein	NA	NA	NA	NA
2591126:2591140	attL	NA	NA	NA	NA
AWC33293.1\|2591242_2591518_-	group-specific protein	S5MC08	Brevibacillus_phage	3.6e-27	59.3
AWC33294.1\|2591544_2592381_-	hypothetical protein	A0A0M5M1L9	Bacillus_phage	4.8e-123	82.7
AWC33295.1\|2592377_2592566_-	transcriptional regulator	A0A1C8E998	Bacillus_phage	8.2e-23	86.9
AWC33296.1\|2592839_2593262_+	transcriptional regulator	A0A1C8E9A4	Bacillus_phage	3.0e-65	90.0
AWC34915.1\|2593276_2593702_+	toxin	A0A0M4RD70	Bacillus_phage	1.7e-71	91.5
AWC33297.1\|2593738_2594896_+\|integrase	site-specific integrase	A0A0S2SXP1	Bacillus_phage	1.2e-76	76.1
2597652:2597666	attR	NA	NA	NA	NA

DBSCAN-SWA_9

2778796 : 2853236

Bacillus_phage(78.72%)

tail,capsid,portal,integrase,protease,holin,terminase,tRNA,head

attL 2810339:2810356|attR 2859957:2859974

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AWC33455.1\|2778796_2781562_-\|tRNA	isoleucine--tRNA ligase	A0A2K9L9X8	Tupanvirus	2.8e-87	27.3
AWC33456.1\|2781906_2782413_-	septum formation initiator	NA	NA	NA	NA
AWC33457.1\|2782505_2783279_-	RNA-binding protein	NA	NA	NA	NA
AWC33458.1\|2783293_2783557_-	YggT family protein	NA	NA	NA	NA
AWC33459.1\|2783563_2784034_-	cell division protein SepF	NA	NA	NA	NA
AWC33460.1\|2784052_2784727_-	YggS family pyridoxal phosphate-dependent enzyme	NA	NA	NA	NA
AWC33461.1\|2784733_2785543_-	laccase domain-containing protein	NA	NA	NA	NA
AWC33462.1\|2785660_2785939_-	YlmC/YmxH family sporulation protein	NA	NA	NA	NA
AWC33463.1\|2786202_2786982_-	RNA polymerase sporulation sigma factor SigG	A0A0Y0AU18	Bacillus_phage	3.4e-46	43.4
AWC33464.1\|2787173_2787893_-	RNA polymerase sporulation sigma factor SigE	A0A2H4JC68	uncultured_Caudovirales_phage	2.5e-19	42.9
AWC33465.1\|2787912_2788839_-	sigma-E processing peptidase SpoIIGA	NA	NA	NA	NA
AWC33466.1\|2789054_2790209_-	cell division protein FtsZ	NA	NA	NA	NA
AWC33467.1\|2790248_2791553_-	cell division protein FtsA	NA	NA	NA	NA
AWC33468.1\|2791953_2792721_-	cell division protein DivIB	NA	NA	NA	NA
AWC34920.1\|2792818_2793724_-	UDP-N-acetylenolpyruvoylglucosamine reductase	NA	NA	NA	NA
AWC33469.1\|2793784_2794879_-	undecaprenyldiphospho-muramoylpentapeptide beta-N- acetylglucosaminyltransferase	NA	NA	NA	NA
AWC34921.1\|2795063_2796155_-	stage V sporulation protein E	NA	NA	NA	NA
AWC33470.1\|2796246_2797602_-	UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase	NA	NA	NA	NA
AWC33471.1\|2797602_2798577_-	phospho-N-acetylmuramoyl-pentapeptide- transferase	NA	NA	NA	NA
AWC33472.1\|2798599_2800075_-	UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2, 6-diaminopimelate ligase	NA	NA	NA	NA
AWC33473.1\|2800347_2802264_-	stage V sporulation protein D	NA	NA	NA	NA
AWC34922.1\|2802361_2804512_-	dihydropteridine reductase	NA	NA	NA	NA
AWC33474.1\|2804534_2804891_-	cell division protein FtsL	NA	NA	NA	NA
AWC33475.1\|2804906_2805839_-	ribosomal RNA small subunit methyltransferase H	NA	NA	NA	NA
AWC34923.1\|2806202_2807819_-	bacillithiol biosynthesis cysteine-adding enzyme BshC	NA	NA	NA	NA
AWC34924.1\|2807898_2808783_-	2-dehydropantoate 2-reductase	NA	NA	NA	NA
AWC33476.1\|2809084_2809558_+	GNAT family N-acetyltransferase	NA	NA	NA	NA
AWC33477.1\|2809653_2810175_-	RsfA family transcriptional regulator	G3MB11	Bacillus_virus	4.0e-11	47.8
2810339:2810356	attL	NA	NA	NA	NA
AWC33478.1\|2810434_2810608_-	50S ribosomal protein L32	NA	NA	NA	NA
AWC33479.1\|2810669_2811170_-	hypothetical protein	NA	NA	NA	NA
AWC33480.1\|2811451_2812072_-	hypothetical protein	Q2LIA9	Bacillus_phage	1.0e-85	76.8
AWC33481.1\|2812013_2813195_-	cell division protein FtsK	Q3HKZ7	Bacillus_phage	3.0e-195	84.5
AWC33482.1\|2813312_2813495_-	hypothetical protein	Q2I8E2	Bacillus_phage	2.2e-20	88.3
AWC33483.1\|2813491_2813800_-	hypothetical protein	A0A288WG08	Bacillus_phage	3.8e-41	78.4
AWC33484.1\|2813983_2814193_+	XRE family transcriptional regulator	Q2I8E4	Bacillus_phage	2.7e-22	79.4
AWC33485.1\|2814195_2814684_+	hypothetical protein	Q3HL01	Bacillus_phage	8.1e-46	61.7
AWC33486.1\|2814719_2815784_-	N-acetylmuramoyl-L-alanine amidase	A0A0A7AQV3	Bacillus_phage	2.3e-178	85.9
AWC33487.1\|2815780_2816020_-\|holin	holin	A0A2H4J378	uncultured_Caudovirales_phage	1.2e-29	87.3
AWC33488.1\|2816019_2816256_-	hypothetical protein	A0A2H4J382	uncultured_Caudovirales_phage	1.2e-07	78.2
AWC33489.1\|2816297_2820944_-	hypothetical protein	A0A1B1P7E6	Bacillus_phage	0.0e+00	62.3
AWC33490.1\|2820940_2822434_-\|tail	phage tail protein	A0A2H4JC38	uncultured_Caudovirales_phage	1.2e-254	83.7
AWC33491.1\|2822442_2826483_-\|tail	phage tail tape measure protein	H0USX3	Bacillus_phage	0.0e+00	75.2
AWC33492.1\|2826699_2827014_-	hypothetical protein	A0A288WFU6	Bacillus_phage	1.8e-35	70.2
AWC33493.1\|2827062_2827674_-\|tail	phage tail protein	A0A2H4J8F3	uncultured_Caudovirales_phage	8.4e-101	94.5
AWC33494.1\|2827674_2828034_-	DUF3168 domain-containing protein	A0A0S2GLE3	Bacillus_phage	1.4e-55	90.8
AWC33495.1\|2828030_2828465_-	hypothetical protein	A0A288WGB7	Bacillus_phage	3.7e-66	85.6
AWC33496.1\|2828457_2828781_-\|head,tail	head-tail adaptor protein	A0A288WG18	Bacillus_phage	8.0e-50	85.0
AWC33497.1\|2828777_2829068_-\|head,tail	phage gp6-like head-tail connector protein	A0A288WGQ6	Bacillus_phage	3.6e-41	88.5
AWC33498.1\|2829085_2830261_-\|capsid	phage major capsid protein	A0A288WG01	Bacillus_phage	2.5e-186	88.0
AWC34925.1\|2830302_2830875_-\|head,protease	HK97 family phage prohead protease	Q2I8F8	Bacillus_phage	1.3e-95	91.1
AWC33499.1\|2830864_2832175_-\|portal	phage portal protein	Q2LIC9	Bacillus_phage	6.4e-215	89.2
AWC33500.1\|2832190_2833888_-\|terminase	terminase large subunit	A0A288WFW5	Bacillus_phage	0.0e+00	92.0
AWC33501.1\|2833884_2834370_-\|terminase	phage terminase small subunit P27 family	A0A288WG26	Bacillus_phage	9.4e-79	96.3
AWC33502.1\|2834469_2834853_-	HNH endonuclease	Q2I8B2	Bacillus_phage	2.2e-62	90.6
AWC34926.1\|2835059_2835425_-	hypothetical protein	A0A288WG64	Bacillus_phage	1.2e-38	75.0
AWC33503.1\|2835680_2835935_-	hypothetical protein	A0A288WG25	Bacillus_phage	2.5e-30	73.8
AWC33504.1\|2835954_2836146_-	hypothetical protein	A0A288WFV5	Bacillus_phage	1.7e-23	88.9
AWC33505.1\|2836151_2836370_-	hypothetical protein	D2XR60	Bacillus_phage	3.3e-23	73.6
AWC33506.1\|2836664_2837042_-	ArpU family transcriptional regulator	Q9ZXB9	Bacillus_phage	1.0e-16	39.7
AWC34927.1\|2837099_2837420_-	hypothetical protein	NA	NA	NA	NA
AWC34928.1\|2837460_2837688_-	hypothetical protein	A0A288WFX6	Bacillus_phage	2.2e-22	68.0
AWC33507.1\|2837733_2838279_-	hypothetical protein	A0A1I9S5V4	Bacillus_phage	3.5e-66	78.0
AWC33508.1\|2838339_2838603_-	hypothetical protein	NA	NA	NA	NA
AWC33509.1\|2838641_2839370_-	RNA polymerase subunit sigma-28	A0A288WFV7	Bacillus_phage	6.4e-55	46.9
AWC33510.1\|2839362_2839761_-	hypothetical protein	NA	NA	NA	NA
AWC33511.1\|2839769_2840660_-	zinc-finger domain-containing protein	NA	NA	NA	NA
AWC33512.1\|2840730_2841441_-	2-methylcitrate dehydratase	NA	NA	NA	NA
AWC33513.1\|2841456_2841873_-	DUF1064 domain-containing protein	A0A2P1JTY5	Anoxybacillus_phage	1.2e-34	56.3
AWC33514.1\|2841865_2842360_-	deoxyuridine 5'-triphosphate nucleotidohydrolase	A0A1B1P8C0	Bacillus_phage	3.2e-58	70.6
AWC33515.1\|2842576_2843029_-	MFS transporter	A0A0A7AQW3	Bacillus_phage	3.3e-33	43.4
AWC33516.1\|2843043_2843526_-	hypothetical protein	NA	NA	NA	NA
AWC33517.1\|2843506_2843701_-	hypothetical protein	NA	NA	NA	NA
AWC34929.1\|2843706_2844399_-	DNA replication protein	A0A0S2GLK2	Bacillus_phage	7.9e-63	50.6
AWC33518.1\|2844469_2845435_-	DnaD domain protein	H0USU2	Bacillus_phage	9.7e-43	51.9
AWC33519.1\|2845609_2846416_-	recombinase RecT	S6AVW6	Thermus_phage	3.4e-97	66.9
AWC33520.1\|2846415_2846655_-	hypothetical protein	NA	NA	NA	NA
AWC33521.1\|2846669_2847605_-	hypothetical protein	S6C475	Thermus_phage	1.9e-99	59.5
AWC33522.1\|2847686_2847902_-	hypothetical protein	NA	NA	NA	NA
AWC34930.1\|2848077_2848371_-	hypothetical protein	A0A0S2GLB6	Bacillus_phage	1.7e-27	62.4
AWC33523.1\|2848378_2848699_-	hypothetical protein	NA	NA	NA	NA
AWC33524.1\|2848846_2849056_-	DNA-binding protein	A0A0U4IIS1	Bacillus_phage	4.4e-09	50.9
AWC34931.1\|2849178_2849388_-	XRE family transcriptional regulator	NA	NA	NA	NA
AWC33525.1\|2849553_2849892_+	XRE family transcriptional regulator	H0UST7	Bacillus_phage	1.3e-21	53.3
AWC33526.1\|2850173_2850308_-	hypothetical protein	NA	NA	NA	NA
AWC33527.1\|2850321_2851419_-	hypothetical protein	NA	NA	NA	NA
AWC33528.1\|2852105_2853236_+\|integrase	site-specific integrase	A0A0U4JNS1	Bacillus_phage	1.7e-62	37.4
2859957:2859974	attR	NA	NA	NA	NA

DBSCAN-SWA_10

3116650 : 3151951

Bacillus_phage(91.43%)

tail,portal,holin,terminase,head

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AWC33813.1\|3116650_3117223_+	DUF4352 domain-containing protein	A0A1B1P898	Bacillus_phage	1.5e-54	84.9
AWC33814.1\|3117533_3117974_+	hypothetical protein	NA	NA	NA	NA
AWC33815.1\|3118048_3118870_-	N-acetylmuramoyl-L-alanine amidase	A0A0S2GLD4	Bacillus_phage	5.9e-142	85.3
AWC33816.1\|3118869_3119310_-\|holin	holin	A0A1B1P7S2	Bacillus_phage	8.3e-66	86.3
AWC33817.1\|3119344_3123838_-	hypothetical protein	A0A0M4RER0	Bacillus_phage	9.5e-266	45.7
AWC33818.1\|3123838_3125332_-\|tail	phage tail protein	A0A1B1P7W7	Bacillus_phage	2.4e-229	76.3
AWC33819.1\|3125344_3128272_-\|tail	phage tail tape measure protein	A0A0A7AR36	Bacillus_phage	2.9e-183	55.7
AWC33820.1\|3128277_3128544_-	hypothetical protein	A0A0M4S6X7	Bacillus_phage	8.3e-21	60.2
AWC33821.1\|3128579_3129017_-	hypothetical protein	A0A0M4QX42	Bacillus_phage	3.6e-13	32.7
AWC33822.1\|3129062_3129695_-	hypothetical protein	A0A0M4R5F9	Bacillus_phage	5.7e-76	79.1
AWC33823.1\|3129712_3130093_-	hypothetical protein	A0A0M4RD75	Bacillus_phage	1.8e-56	83.3
AWC33824.1\|3130089_3130506_-	hypothetical protein	A0A0M4S6Z2	Bacillus_phage	2.7e-58	76.8
AWC33825.1\|3130489_3130849_-	hypothetical protein	A0A0M4S6Z9	Bacillus_phage	5.7e-49	81.2
AWC33826.1\|3130830_3131169_-	hypothetical protein	W8CYS9	Bacillus_phage	2.9e-10	39.6
AWC33827.1\|3131205_3132048_-	hypothetical protein	A0A0M5M1L4	Bacillus_phage	5.2e-117	77.7
AWC33828.1\|3132061_3132652_-	DUF4355 domain-containing protein	A0A0M3ULE7	Bacillus_phage	4.6e-19	55.8
AWC33829.1\|3132721_3133747_-\|head	phage head morphogenesis protein	A0A0M5M7Z2	Bacillus_phage	5.1e-159	84.1
AWC33830.1\|3133733_3135089_-\|portal	phage portal protein	A0A0M4S669	Bacillus_phage	3.5e-200	85.6
AWC33831.1\|3135100_3136405_-\|terminase	PBSX family phage terminase large subunit	A0A0M4R5F3	Bacillus_phage	9.5e-251	95.2
AWC33832.1\|3136391_3136823_-\|terminase	terminase small subunit	A0A0M4RU19	Bacillus_phage	1.3e-55	74.8
AWC33833.1\|3138233_3138614_-	ArpU family transcriptional regulator	A0A0M4R397	Bacillus_phage	1.1e-53	84.7
AWC33834.1\|3138618_3138906_-	hypothetical protein	A6M999	Geobacillus_virus	2.9e-35	73.4
AWC34942.1\|3139285_3139537_-	XRE family transcriptional regulator	A0A0S2MV80	Bacillus_phage	1.3e-36	92.7
AWC33835.1\|3139788_3140073_-	hypothetical protein	I7J4K9	Bacillus_phage	4.3e-15	48.9
AWC33836.1\|3140173_3140383_+	hypothetical protein	NA	NA	NA	NA
AWC33837.1\|3140443_3140875_-	hypothetical protein	W8CYU3	Bacillus_phage	2.6e-19	39.9
AWC33838.1\|3140904_3141108_-	hypothetical protein	NA	NA	NA	NA
AWC33839.1\|3141129_3141663_-	hypothetical protein	U5PUK4	Bacillus_phage	8.8e-54	60.0
AWC33840.1\|3141705_3141912_-	hypothetical protein	NA	NA	NA	NA
AWC34943.1\|3142226_3142799_-	dUTPase	A0A0A7AQI4	Bacillus_phage	1.7e-82	78.1
AWC33841.1\|3142951_3143575_-	hypothetical protein	A0A0M4RU33	Bacillus_phage	2.3e-29	42.1
AWC33842.1\|3144922_3145513_-	hypothetical protein	A0A0M4S6Y4	Bacillus_phage	3.2e-36	60.1
AWC33843.1\|3146670_3147153_-	ATPase	E5DV79	Deep-sea_thermophilic_phage	8.3e-35	48.1
AWC33844.1\|3147606_3147873_-	hypothetical protein	A0A0M4S677	Bacillus_phage	2.3e-23	80.9
AWC33845.1\|3148416_3149193_-	hypothetical protein	A0A288WFS9	Bacillus_phage	2.7e-59	62.9
AWC33846.1\|3149189_3149378_-	XRE family transcriptional regulator	A0A0M3ULF9	Bacillus_phage	1.1e-22	91.4
AWC33847.1\|3149650_3150073_+	XRE family transcriptional regulator	A0A0M4QX29	Bacillus_phage	2.0e-69	97.1
AWC34944.1\|3150087_3150513_+	ImmA/IrrE family metallo-endopeptidase	A0A0M4RD70	Bacillus_phage	7.5e-72	92.2
AWC33848.1\|3150526_3151951_+	recombinase family protein	A0A1L2JY12	Aeribacillus_phage	7.2e-103	42.4

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

Overview of predicted results

Overview of the results

Cas Category Instructions

Results visualization

1. CP024102

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection

2. CP024103

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection

3. CP024101

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Click the colored protein region to show detailed information

Click the colored protein region to show detailed information

Click the colored protein region to show detailed information

Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection