CRISPRimmunity

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Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Target MGE spacer number	Prophage number
CP026546	Cupriavidus metallidurans strain Ni-2 plasmid unnamed2	0 crisprs	NA	0	1
CP026547	Cupriavidus metallidurans strain Ni-2 plasmid unnamed3	1 crisprs	csa3	6	1
CP026544	Cupriavidus metallidurans strain Ni-2 chromosome, complete genome	0 crisprs	csa3,RT,DinG,DEDDh,cas3,PD-DExK,WYL	0	13
CP026545	Cupriavidus metallidurans strain Ni-2 plasmid unnamed1	0 crisprs	NA	0	0

Results visualization

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CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

96948 : 148177

Mycobacterium_phage(14.29%)

protease,integrase,transposase

attL 112796:112812|attR 130215:130231

The bacterium proteins that are colored denote the protein is present at specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin' and 'tRNA')

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AVA38086.1\|96948_98118_+\|protease	metalloprotease	NA	NA	NA	NA
AVA38087.1\|98182_98362_-	hypothetical protein	NA	NA	NA	NA
AVA38088.1\|98358_98631_-	hypothetical protein	NA	NA	NA	NA
AVA38089.1\|99385_100009_+	hypothetical protein	NA	NA	NA	NA
AVA38090.1\|101250_101499_+	hypothetical protein	NA	NA	NA	NA
AVA38091.1\|101495_101720_+	hypothetical protein	NA	NA	NA	NA
AVA38092.1\|101726_102125_+	hypothetical protein	NA	NA	NA	NA
AVA38093.1\|102365_102689_-	hypothetical protein	NA	NA	NA	NA
AVA38094.1\|102754_102982_-	hypothetical protein	NA	NA	NA	NA
AVA38095.1\|103059_103704_-	hypothetical protein	NA	NA	NA	NA
AVA38096.1\|103720_103912_-	hypothetical protein	NA	NA	NA	NA
AVA38097.1\|104045_104624_-	hypothetical protein	NA	NA	NA	NA
AVA38098.1\|104671_105400_-	hypothetical protein	NA	NA	NA	NA
AVA38099.1\|105661_106189_+	hypothetical protein	NA	NA	NA	NA
AVA38100.1\|106529_107063_-	hypothetical protein	NA	NA	NA	NA
AVA38101.1\|107186_107921_-	hypothetical protein	NA	NA	NA	NA
AVA38102.1\|107985_108927_-	hypothetical protein	NA	NA	NA	NA
AVA38103.1\|108899_109304_-	hypothetical protein	NA	NA	NA	NA
AVA38104.1\|109434_109671_-	hypothetical protein	NA	NA	NA	NA
AVA38105.1\|110119_110476_-	hypothetical protein	NA	NA	NA	NA
AVA38106.1\|110498_110777_-	hypothetical protein	NA	NA	NA	NA
AVA38107.1\|111036_112473_-	SAM-dependent methyltransferase	NA	NA	NA	NA
112796:112812	attL	NA	NA	NA	NA
AVA38108.1\|112914_113217_+	hypothetical protein	NA	NA	NA	NA
AVA38109.1\|113219_113699_+	DUF2726 domain-containing protein	NA	NA	NA	NA
AVA38110.1\|113722_114103_+	hypothetical protein	NA	NA	NA	NA
AVA38111.1\|114282_114588_+	hypothetical protein	NA	NA	NA	NA
AVA38112.1\|114636_115485_+	XRE family transcriptional regulator	NA	NA	NA	NA
AVA38113.1\|115991_116831_+	hypothetical protein	NA	NA	NA	NA
AVA38114.1\|116888_117614_-	hypothetical protein	NA	NA	NA	NA
AVA38115.1\|117830_118217_+	hypothetical protein	NA	NA	NA	NA
AVA38116.1\|118240_118498_+	hypothetical protein	NA	NA	NA	NA
AVA38117.1\|118516_118888_+	hypothetical protein	NA	NA	NA	NA
AVA38118.1\|119101_119473_-	hypothetical protein	NA	NA	NA	NA
AVA38119.1\|119608_119884_-	hypothetical protein	NA	NA	NA	NA
AVA38120.1\|120116_121373_+	site-specific DNA-methyltransferase	NA	NA	NA	NA
AVA38121.1\|121664_121952_+	DNA-binding protein	NA	NA	NA	NA
AVA38122.1\|122060_122447_+	hypothetical protein	NA	NA	NA	NA
AVA38123.1\|122620_123100_+	hypothetical protein	NA	NA	NA	NA
AVA38124.1\|123326_123890_+	hypothetical protein	NA	NA	NA	NA
AVA38125.1\|124060_125032_+	hypothetical protein	A0A1D8EVK5	Mycobacterium_phage	8.1e-13	29.3
AVA38126.1\|125434_126613_+\|integrase	site-specific integrase	NA	NA	NA	NA
AVA38127.1\|126609_128103_+	hypothetical protein	NA	NA	NA	NA
AVA38128.1\|128099_130028_+	hypothetical protein	NA	NA	NA	NA
AVA38129.1\|130024_130507_+	hypothetical protein	NA	NA	NA	NA
130215:130231	attR	NA	NA	NA	NA
AVA38130.1\|130724_131609_-	LysR family transcriptional regulator	NA	NA	NA	NA
AVA38131.1\|131605_132616_-	hydroxyacid dehydrogenase	A0A1V0SBV6	Catovirus	5.6e-25	28.8
AVA38192.1\|132640_133438_-	phosphonate ABC transporter, permease protein PhnE	NA	NA	NA	NA
AVA38132.1\|133450_134311_-	phosphate/phosphite/phosphonate ABC transporter substrate-binding protein	NA	NA	NA	NA
AVA38193.1\|134313_135132_-	phosphonate ABC transporter ATP-binding protein	G9BWD6	Planktothrix_phage	5.4e-26	37.2
AVA38133.1\|135908_136778_-\|transposase	IS5/IS1182 family transposase	A0A077K814	Ralstonia_phage	7.5e-103	69.3
AVA38134.1\|137218_137917_+	Urease subunit alpha	NA	NA	NA	NA
AVA38135.1\|138862_139174_+\|transposase	transposase	NA	NA	NA	NA
AVA38136.1\|139285_140725_+\|transposase	IS481 family transposase	O12274	Simian_T-lymphotropic_virus	4.1e-05	37.8
AVA38137.1\|140721_141555_+	general secretion pathway protein GspA	NA	NA	NA	NA
AVA38138.1\|141551_141824_+	hypothetical protein	NA	NA	NA	NA
AVA38139.1\|142095_142968_-	LysR family transcriptional regulator	A0A2H4J8I9	uncultured_Caudovirales_phage	7.3e-05	37.8
AVA38140.1\|142960_143986_-	hydroxyacid dehydrogenase	M1NSB9	Streptococcus_phage	5.7e-17	26.0
AVA38141.1\|144698_145115_-	DUF1772 domain-containing protein	NA	NA	NA	NA
AVA38142.1\|147865_148177_+\|transposase	transposase	NA	NA	NA	NA

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

2. CP026547

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CRISPR-Cas detection and classification

Crispr_ID: CP026547_1

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

CP026547_1

116118-116362

Orphan

Consensus_repeat	Method
GGTGTTTGACGGTCAAACACCC	PILER-CR
GGTGTTTGACGGTCAAACACCCC	CRISPRCasFinder

3 spacers

The CRISPR arrays of CP026547_1

>merge|CP026547|1|116118-116362|PILER-CR,CRISPRCasFinder
GGTGTTTGACGGTCAAACACCTGCGTGGATTTCGCTTGCAGCGTCGCTTCAGTCAGTTCCCAGAAGAGGTGTTTGACGGTCAAACACCCTTCCCGAGTCCTCTCCGCCTATTCGCGCAGCGTCACTGCAGTGGGTGCGCTGCAGGGGGTGTTTGACGGTCAAACACCCCAAAAGCCGTCTTCATCAACTCGCATAGCATCAATGCAGCGGGATCGCTGCAGAGGTGTTTGACGGTCAAACACCCC

>CP026547|1|1|116118-116361|PILER-CR
GGTGTTTGACGGTCAAACACCT	GCGTGGATTTCGCTTGCAGCGTCGCTTCAGTCAGTTCCCAGAAGA
GGTGTTTGACGGTCAAACACCC	TTCCCGAGTCCTCTCCGCCTATTCGCGCAGCGTCACTGCAGTGGGTGCGCTGCAGGG
GGTGTTTGACGGTCAAACACCC	CAAAAGCCGTCTTCATCAACTCGCATAGCATCAATGCAGCGGGATCGCTGCAGA
GGTGTTTGACGGTCAAACACCC

>CP026547|1|1|116118-116362|CRISPRCasFinder
GGTGTTTGACGGTCAAACACCTG	CGTGGATTTCGCTTGCAGCGTCGCTTCAGTCAGTTCCCAGAAGA
GGTGTTTGACGGTCAAACACCCT	TCCCGAGTCCTCTCCGCCTATTCGCGCAGCGTCACTGCAGTGGGTGCGCTGCAGGG
GGTGTTTGACGGTCAAACACCCC	AAAAGCCGTCTTCATCAACTCGCATAGCATCAATGCAGCGGGATCGCTGCAGA
GGTGTTTGACGGTCAAACACCCC

Protein	Signature genes	Signature genes Name	Protein_function
CP026547.1\|AVA38330.1\|120540_121272_+\|hypothetical-protein	unknown	unknown	gnl\|CDD\|349751
CP026547.1\|AVA38332.1\|121516_121924_+\|hypothetical-protein	unknown	unknown	unknown
CP026547.1\|AVA38319.1\|110796_111000_-\|hypothetical-protein	unknown	unknown	unknown
CP026547.1\|AVA38333.1\|122075_123839_+\|hypothetical-protein	unknown	unknown	unknown
CP026547.1\|AVA38326.1\|112903_114721_+\|integrase	unknown	unknown	gnl\|CDD\|271175
CP026547.1\|AVA38318.1\|109359_109806_-\|hypothetical-protein	unknown	unknown	unknown
CP026547.1\|AVA38329.1\|119710_120508_+\|hypothetical-protein	unknown	unknown	gnl\|CDD\|349757
CP026547.1\|AVA38335.1\|124316_124655_+\|hypothetical-protein	unknown	unknown	unknown
CP026547.1\|AVA38328.1\|117983_119537_+\|hypothetical-protein	unknown	unknown	gnl\|CDD\|376444
CP026547.1\|AVA38325.1\|112369_112690_-\|hypothetical-protein	unknown	unknown	unknown
CP026547.1\|AVA38389.1\|116375_117509_-\|cobyrinic-acid-a,c-diamide-synthase	unknown	unknown	gnl\|CDD\|274585
CP026547.1\|AVA38331.1\|121265_121520_+\|hypothetical-protein	unknown	unknown	unknown
CP026547.1\|AVA38334.1\|123846_124173_+\|hypothetical-protein	unknown	unknown	unknown
CP026547.1\|AVA38324.1\|112162_112345_-\|hypothetical-protein	unknown	unknown	unknown
CP026547.1\|AVA38327.1\|115044_116088_-\|hypothetical-protein	unknown	unknown	gnl\|CDD\|319262
CP026547.1\|AVA38320.1\|111070_111307_-\|hypothetical-protein	unknown	unknown	unknown
CP026547.1\|AVA38323.1\|111821_112100_-\|hypothetical-protein	unknown	unknown	unknown
CP026547.1\|AVA38321.1\|111312_111504_-\|hypothetical-protein	unknown	unknown	unknown
CP026547.1\|AVA38322.1\|111547_111796_-\|hypothetical-protein	unknown	unknown	unknown
CP026547.1\|AVA38336.1\|124721_125201_+\|hypothetical-protein	unknown	unknown	unknown

Protein	Function_ID	Function_description	E-value
CP026547.1\|AVA38389.1\|116375_117509_-\|cobyrinic-acid-a,c-diamide-synthase	gnl\|CDD\|274585	TIGR03453, partition_RepA, plasmid partitioning protein RepA. Members of this family are the RepA (or ParA) protein involved in replicon partitioning. All known examples occur in bacterial species with two or more replicons, on a plasmid or the smaller chromosome. Note that an apparent exception may be seen as a pseudomolecule from assembly of an incompletely sequenced genome. Members of this family belong to a larger family that also includes the enzyme cobyrinic acid a,c-diamide synthase, but assignment of that name to members of this family would be in error. [Mobile and extrachromosomal element functions, Plasmid functions].	1.11381e-61
CP026547.1\|AVA38330.1\|120540_121272_+\|hypothetical-protein	gnl\|CDD\|349751	cd01983, SIMIBI, SIMIBI (signal recognition particle, MinD and BioD)-class NTPases. SIMIBI (after signal recognition particle, MinD, and BioD), consists of signal recognition particle (SRP) GTPases, the assemblage of MinD-like ATPases, which are involved in protein localization, chromosome partitioning, and membrane transport, and a group of metabolic enzymes with kinase or related phosphate transferase activity. Functionally, proteins in this superfamily use the energy from hydrolysis of NTP to transfer electron or ion.	0.000299799
CP026547.1\|AVA38326.1\|112903_114721_+\|integrase	gnl\|CDD\|271175	cd00397, DNA_BRE_C, DNA breaking-rejoining enzymes, C-terminal catalytic domain. The DNA breaking-rejoining enzyme superfamily includes type IB topoisomerases and tyrosine based site-specific recombinases (integrases) that share the same fold in their catalytic domain containing conserved active site residues. The best-studied members of this diverse superfamily include Human topoisomerase I, the bacteriophage lambda integrase, the bacteriophage P1 Cre recombinase, the yeast Flp recombinase, and the bacterial XerD/C recombinases. Their overall reaction mechanism is essentially identical and involves cleavage of a single strand of a DNA duplex by nucleophilic attack of a conserved tyrosine to give a 3' phosphotyrosyl protein-DNA adduct. In the second rejoining step, a terminal 5' hydroxyl attacks the covalent adduct to release the enzyme and generate duplex DNA. The enzymes differ in that topoisomerases cleave and then rejoin the same 5' and 3' termini, whereas a site-specific recombinase transfers a 5' hydroxyl generated by recombinase cleavage to a new 3' phosphate partner located in a different duplex region. Many DNA breaking-rejoining enzymes also have N-terminal domains, which show little sequence or structure similarity.	2.81055e-26
CP026547.1\|AVA38329.1\|119710_120508_+\|hypothetical-protein	gnl\|CDD\|349757	cd02037, Mrp_NBP35, Mrp/NBP35 ATP-binding protein family. Mrp/NBP35 ATP-binding family protein are typically iron-sulfur (FeS) cluster scaffolds that function to assemble nascent FeS clusters for transfer to FeS-requiring enzymes. Members include the eukaryotic nucleotide-binding protein 1 (NUBP1) which is a component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery and the archael [NiFe] hydrogenase maturation protein HypB which is required for nickel insertion into [NiFe] hydrogenase.	1.00991e-09
CP026547.1\|AVA38327.1\|115044_116088_-\|hypothetical-protein	gnl\|CDD\|319262	cd16405, RepB_like_N, plasmid segregation replication protein B like protein, N-terminal domain. RepB, found on plasmids and secondary chromosomes, works along with repA in directing plasmid segregation, and has been shown in Rhizobium etli to require the parS centromere-like sequence for full transcriptional repression of the repABC operon, inducing plasmid incompatibility. RepA is a Walker-type ATPase that complexes with RepB to form DNA-protein complexes in the presence of ATP/ADP. RepC is an initiator protein for the plasmid. repA and repB are homologous to the parA and ParB genes of the parABS partitioning system found on primary chromosomes.	6.95019e-25
CP026547.1\|AVA38328.1\|117983_119537_+\|hypothetical-protein	gnl\|CDD\|376444	pfam01051, Rep_3, Initiator Replication protein. This protein is an initiator of plasmid replication. RepB possesses nicking-closing (topoisomerase I) like activity. It is also able to perform a strand transfer reaction on ssDNA that contains its target. This family also includes RepA which is an E.coli protein involved in plasmid replication. The RepA protein binds to DNA repeats that flank the repA gene.	2.43474e-16

>CP026547.1|AVA38327.1|115044_116088_-|hypothetical-protein
MTKKRLGTLVGEIDMSAYEKRAGNAGASAATRPQTGVGMVMGAIAGNSDIERKLTETQASLDQASQRLAEYEGAELVRKLDPSSIRRSRWANRDELNFKGPDWEAFKAELQNAGGNVEPIKVRRVFDGQTPPKDGGTAPEYEVVYGHRRHQGCWELTLPVNAIVVESMDDRDLFAEMDRENRQRQNLSAWEQGRMYNHALKEGLYPSLRWLADELGVNLSDASRCCKLAQLPDAVVQAFPSPMVIQVRWAKPLADALQSDPDGMLERARAIQADRDSLTPAEVIERLLNQPAKSQKPSIPIRARGQTAASLRIGPKGRAVVEFEAGVLEPARHDALVKLIADFLSAS
>CP026547.1|AVA38326.1|112903_114721_+|integrase
MRKPLSTIERLGKHHFAHLKAVAMGVPFQAAALRYLGIHHGHQAAGVHRLTVESVRALARRQHEPAWRLIGLVIRVDAETQQGPSLAEFIASRGLEDWSEEDVGRFYLEAYPEQAEEAGSARAKRRERSRSAQLELLEKLEKQSVVPPSPVDPVATWFDPVTAQRLVGAGMVSLLDLADRIAVGGRWFGGMPGVGVNKAERIAAFLRDLLGDTYPLQRHFVVPLHSEAAALPQTGLPDVVEAAQQLPSQLTYEPYSLLAARSDADAIEEWIAAKAGSKQTALGYRREARRLLLWLLEECGGKGLGEMTASDCAQYAAFLADIPLRWQSKARAKPGAEGWAPFRGPLSHQSRQYALNVLGTLFTWLQAAQYIPSNPWVLVNRKLGDDPAARKLDTKAIGEGAMAEILRVINAQPDSPSRTRILFILSFVEAVGLRSQELISARLKDFSLEPEGWVLHVHGKGAKNRVAAVPGQALDALQAYLRERGLGGIETASGDFPLLGSLGDSSQSVGYQALHEHVKGWLKKAVARSALPEHERRKLAGASTHWLRHTFGTRAVARNVPLDVIQEQLGHASIQTTMNIYGRAPIQRRTAELGKAFDTPSTADT
>CP026547.1|AVA38325.1|112369_112690_-|hypothetical-protein
MQAIESITTAQMPIRRTTTQRVIYCGRTATCCEYLLQSGSTGLRVVSRHFDGGTTTEVRLIYGDGRTAKFREYLSANVAPELVDATVADFAAKAAEQHAIKKHGFH
>CP026547.1|AVA38324.1|112162_112345_-|hypothetical-protein
MYANEIATGRNWYTADRAKLVTFNDCARAELHWNDGGYAYSLFDTEAEARQYLREHGFNA
>CP026547.1|AVA38323.1|111821_112100_-|hypothetical-protein
MLIRSKNPLASFNRRSREEKDFAIADGCQITREWNVEGYECWHAEKPQAVLKSSLPGNRAEAGACVNDAPRIWYTYPGCGHVFETVIVSRTA
>CP026547.1|AVA38322.1|111547_111796_-|hypothetical-protein
MKTIERLLRRIAYVGTSWIVDGPTPPGFTGWRSRVLHAGIAFGWMSRGGRYDDRTLYRRTIGNASARAHFRFAFTGKFRRAS
>CP026547.1|AVA38321.1|111312_111504_-|hypothetical-protein
MSARAVQSRIDKAAQEFAALIRDDHWTLQHAHDVLIHADRDGLDLFSIAGLAKAIACYQCSEE
>CP026547.1|AVA38320.1|111070_111307_-|hypothetical-protein
MSVALHRVQDLSAASAALGAKEELQLAVSTYCEWRDRQIDAVEDEDTHLAAFIGRNMKALRERFDVSQWEREIARQDA
>CP026547.1|AVA38319.1|110796_111000_-|hypothetical-protein
MGGHGAISPRRYKPALAKHAAWAKQPHHVYLHTNSGTVAAVAFGGIEGSIALLEQMAGPEFVGPRFA
>CP026547.1|AVA38318.1|109359_109806_-|hypothetical-protein
MARTQALAGGEIGINGHHYKGGQFLPSTQAEPGRWKVGGKWVMTGRRLVAPGEFALQPTPFSVALFQLAGVGYATTLDEHGRLVLNPGANGDGVRDYDGNQITRDTEVRPGIKGILGKDATTIGAIVDAWNDGMRWFEVTPDAETKTA
>CP026547.1|AVA38389.1|116375_117509_-|cobyrinic-acid-a,c-diamide-synthase
MMDAVRGKLLAPDSRKESPRFNATQLADLCGVDRNAIAYRSKKDDHLPKGQINGSGNRREFALPEVHAWVREYRANYLKPAGHDAVCIAVANFKGGVGKTTTTMTLAQGLSLKGHRVLVVDCDPQGSLTTLFGILPATEVSEEDTILPVCMGTEPSISSAIRESYWHGIDVVGAAPLLFAAEFALPARQMRDPSFQFWDVLNLALDEARQNYDVILVDTPPALSYVTINAIMASDGILMPLPPNALAVASAAQFWRLFSDLAAQLVEQRGVTKEFDFVRVLMTQVKVRGKIEADDTITAVKDWIYKTYEGKVLSVEIPETKATETSAANFGTIYDESPKLKEMIDPRTYKRALSAYDQATEQIEALIRAAWQRHRTN
>CP026547.1|AVA38328.1|117983_119537_+|hypothetical-protein
MTQIETFDAQSCVGPGSQLSFPRAAGCGYVSPIGGRSGHGTSKMREVCGLIQLTHVGCHVRMKQFELYPVDAPEARDELLRKHVNAIALMPTKDGGKISAFDRTLYDVLLHRAQGMGDQDEYAARLHEIVKDLDTNSHNTEQIKKSLKNLMKTIVEWQSPTSGEIEVWDACVLLSGASIRKDKRTQSVEVRWRYDTQIKAQLLDPDRYAGLLLKSITQLRSHPAKALYGICARYVDNPGRKTARQHWRWWRPVLCGQVYDENKGEYRYFKRDVLQPAIAEINANTELEVSLLPEFKERDNKTVSDIQFEVRLKGLARKKPQAPGSKPLDKVEPADLKLIGQALKLGVAQVEAEGLYREHGAEALQKGLGDLEKRVAMPAQVVGAVEKPGSYLRSLMRPKAAAQVPASEAIPKARPGKDLERSKAALLEEWLRQKKDELRSLFQELPEVEQAELLAAFRTYPQVQPLLKRFETAGWNHKTIRDIFAAFLGETWLGAEWNKPKPDELLALALEKQTTAA
>CP026547.1|AVA38329.1|119710_120508_+|hypothetical-protein
MAKAPVETSQPVSKFIVVHGDKGGVGKSMVAQALADQLMCSGAKVAIVEADTRNPDVARMFGNNLPVAHTNVRSENGWMDVMDFVMKYPGHTIIMNTPAGIGEYMRSDMESFAKFLKAQDMPVEMELWWVMNVQHDSVNLLNEAYTAYGAFFSRIRVVCNLHYAGGDKSQHGPFVLWHESPLKAQIEKKQGLTIFFPGLHIRVVAKVLDPRKIMPFSDAVDAVVGESVALEHSERWKLQQWLADCQKAFEPAIGPAVAPAVANAG
>CP026547.1|AVA38330.1|120540_121272_+|hypothetical-protein
MVSGNKGGVGKSVFCLAFASALEMRGEPFAVFDGDGRTGDVFATFRRKCPARLGDFRALRPESHLCNLDLIYETQLQQLLAGSPHLIVNTPDGADAVLKKWFDVTLSHTEASNINFRFVYLMSDRPDGLEMLPQLAESFQFLYPVRNLHFGPERRFTAFNQQYLDGFKVVLDLPALRGEEVRMLFDLQTFPSEAMKLKKKGTGTYAIPTLSRARLHAWQRQVNELVWDMVDNTEMPNLEFGKW
>CP026547.1|AVA38331.1|121265_121520_+|hypothetical-protein
MVMAAESFARIASHHWIRHTDQPIFHAYISIRPGTTSVCGEGRAFQSVREMDVPGERSQCCAKCCEALYGRIGYRPPLSGEDFS
>CP026547.1|AVA38332.1|121516_121924_+|hypothetical-protein
MSLSLISALEALKKQPLTAEEAAMVQEFQRQYELDDDDPLNVVLAMMARSQMIIETAPDLLQQKVVETIELHRTNLREQAVLSAKELITDLSALLLSQQKGVSAIWRQRLSWYASGAASMLLVMGSALAVARWWH
>CP026547.1|AVA38333.1|122075_123839_+|hypothetical-protein
MRAYQTYGFDDASTPGNDQTFRHIDTGVLLLHDGDVVAIQFGDGNLSAALSDLFLVLHGLGWNEAEVFHPAETLGALLEDMGKAIPGHMEFDVKAAKTVADTGRVPEETALIERGKSLLQGVAVGHQEVQDFNTVALGELESMTTDHTFHSVVAADTSAPLQSQPAMEAEPISAPVHLDDVFDLGIGDALGQSLSEPAHAIRPHGQVFLDDDDSGPVVPVLGASTLQSAAAHPSQSGGEEVYASAGLPIDATVDATAQPNAQPVTVEEPGPVEQPAGAPTSHPVRIAQTESVPVPDARIDTPVAHVPTFVARSGKSSAPLTLIRVGRSALCFDLPEAPVSLEEVSQCAGQLDANEVVHLWPGLIHQSERWDLVGEIDLNAPWFAEVVASELAPQCAVERVWFAGALLQLARRQGGAQLRDMLVGLLHADGAESCGAISQAAPFKPDAHERFVNLVLDRFSGLLLCQEGESFLDVRSADGTAAGLEIAVRAFSVRSMVEAADPKLYVIHLDATDGPFIVMIVRLLRGVAERYSISTRARTQAQALQHEVERREASQREAERLSAVEMVQSTMAGLMEQLKKAGIAVPT
>CP026547.1|AVA38334.1|123846_124173_+|hypothetical-protein
MEIDLQGELDACLGAAEKVLDGLPEPLSDGAKVDPAVRARLQWIERQLLNMTAKLKAMQEDMEAGLNLNEMGFADPQEMLDLLNDMSVQIEQLKAMCLALGRNLARGL
>CP026547.1|AVA38335.1|124316_124655_+|hypothetical-protein
MKKVLIVAATVAMASAAQAQVVLKPTYVEAPVQGTSCELPSGLVLPFLNYDASGRQMQCIGNAMGEKPTWRYVAESDSERIGRKLDQLNATNVAILTKLAELIAVQQASAQK
>CP026547.1|AVA38336.1|124721_125201_+|hypothetical-protein
MYRIDPARPMCIYGSVTRDLSKMTQDPMHDLVMDLAQQTLNPGFLRLADPVNEASFDVLPAEFLEWLIGVSRWPNEFFEHLQCVTQTSEGEPLLTTWLDADRFWSMLSDGRGVPVVEAISARVDEWRSLGLDEVYFDFYVLGSSAVHAQAENSASLVTV

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Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Identity
CP026547_1	1.1\|116140\|45\|CP026547\|PILER-CR	116140-116184	45	NZ_CP026547	Cupriavidus metallidurans strain Ni-2 plasmid unnamed3	116140-116184	1.0
CP026547_1	1.2\|116207\|57\|CP026547\|PILER-CR	116207-116263	57	NZ_CP026547	Cupriavidus metallidurans strain Ni-2 plasmid unnamed3	116207-116263	1.0
CP026547_1	1.3\|116286\|54\|CP026547\|PILER-CR	116286-116339	54	NZ_CP026547	Cupriavidus metallidurans strain Ni-2 plasmid unnamed3	116286-116339	1.0
CP026547_1	1.4\|116141\|44\|CP026547\|CRISPRCasFinder	116141-116184	44	NZ_CP026547	Cupriavidus metallidurans strain Ni-2 plasmid unnamed3	116141-116184	1.0
CP026547_1	1.5\|116208\|56\|CP026547\|CRISPRCasFinder	116208-116263	56	NZ_CP026547	Cupriavidus metallidurans strain Ni-2 plasmid unnamed3	116208-116263	1.0
CP026547_1	1.6\|116287\|53\|CP026547\|CRISPRCasFinder	116287-116339	53	NZ_CP026547	Cupriavidus metallidurans strain Ni-2 plasmid unnamed3	116287-116339	1.0

1. spacer 1.1|116140|45|CP026547|PILER-CR matches to NZ_CP026547 (Cupriavidus metallidurans strain Ni-2 plasmid unnamed3) position: , mismatch: 0, identity: 1.0

gcgtggatttcgcttgcagcgtcgcttcagtcagttcccagaaga	CRISPR spacer
gcgtggatttcgcttgcagcgtcgcttcagtcagttcccagaaga	Protospacer
*********************************************

2. spacer 1.2|116207|57|CP026547|PILER-CR matches to NZ_CP026547 (Cupriavidus metallidurans strain Ni-2 plasmid unnamed3) position: , mismatch: 0, identity: 1.0

ttcccgagtcctctccgcctattcgcgcagcgtcactgcagtgggtgcgctgcaggg	CRISPR spacer
ttcccgagtcctctccgcctattcgcgcagcgtcactgcagtgggtgcgctgcaggg	Protospacer
*********************************************************

3. spacer 1.3|116286|54|CP026547|PILER-CR matches to NZ_CP026547 (Cupriavidus metallidurans strain Ni-2 plasmid unnamed3) position: , mismatch: 0, identity: 1.0

caaaagccgtcttcatcaactcgcatagcatcaatgcagcgggatcgctgcaga	CRISPR spacer
caaaagccgtcttcatcaactcgcatagcatcaatgcagcgggatcgctgcaga	Protospacer
******************************************************

4. spacer 1.4|116141|44|CP026547|CRISPRCasFinder matches to NZ_CP026547 (Cupriavidus metallidurans strain Ni-2 plasmid unnamed3) position: , mismatch: 0, identity: 1.0

cgtggatttcgcttgcagcgtcgcttcagtcagttcccagaaga	CRISPR spacer
cgtggatttcgcttgcagcgtcgcttcagtcagttcccagaaga	Protospacer
********************************************

5. spacer 1.5|116208|56|CP026547|CRISPRCasFinder matches to NZ_CP026547 (Cupriavidus metallidurans strain Ni-2 plasmid unnamed3) position: , mismatch: 0, identity: 1.0

tcccgagtcctctccgcctattcgcgcagcgtcactgcagtgggtgcgctgcaggg	CRISPR spacer
tcccgagtcctctccgcctattcgcgcagcgtcactgcagtgggtgcgctgcaggg	Protospacer
********************************************************

6. spacer 1.6|116287|53|CP026547|CRISPRCasFinder matches to NZ_CP026547 (Cupriavidus metallidurans strain Ni-2 plasmid unnamed3) position: , mismatch: 0, identity: 1.0

aaaagccgtcttcatcaactcgcatagcatcaatgcagcgggatcgctgcaga	CRISPR spacer
aaaagccgtcttcatcaactcgcatagcatcaatgcagcgggatcgctgcaga	Protospacer
*****************************************************

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

8324 : 57011

Salmonella_phage(20.0%)

transposase,integrase

attL 41621:41637|attR 52101:52117

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AVA38208.1\|8324_10010_-\|integrase	integrase	A0A0K2CP59	Brevibacillus_phage	8.8e-07	26.8
AVA38209.1\|10111_11680_-	hypothetical protein	NA	NA	NA	NA
AVA38210.1\|11676_12564_-	hypothetical protein	NA	NA	NA	NA
AVA38211.1\|12560_15500_-	DUF4158 domain-containing protein	NA	NA	NA	NA
AVA38212.1\|15620_15971_-	transcriptional regulator	NA	NA	NA	NA
AVA38213.1\|16158_16719_+	recombinase family protein	A0A1B0V7I5	Salmonella_phage	9.6e-51	88.6
AVA38214.1\|16721_19691_+\|transposase	Tn3 family transposase	A0A1B0V7H9	Salmonella_phage	0.0e+00	72.3
AVA38215.1\|20386_21346_+\|transposase	IS5/IS1182 family transposase	A0A1V0E8E1	Vibrio_phage	1.8e-65	45.3
AVA38216.1\|21432_22452_+\|transposase	IS30 family transposase	W5R8L2	Staphylococcus_phage	3.2e-44	36.4
AVA38383.1\|23198_23540_-	hypothetical protein	NA	NA	NA	NA
AVA38217.1\|23612_24323_-	hypothetical protein	NA	NA	NA	NA
AVA38218.1\|24319_25606_-	ABC transporter permease	NA	NA	NA	NA
AVA38219.1\|25688_26468_-	ABC transporter	A0A2H4PQG7	Staphylococcus_phage	6.9e-15	26.3
AVA38220.1\|26546_26729_+	hypothetical protein	NA	NA	NA	NA
AVA38221.1\|27339_28158_+	molybdenum-dependent transcriptional regulator	NA	NA	NA	NA
AVA38222.1\|28322_29243_+	ABC transporter substrate-binding protein	NA	NA	NA	NA
AVA38223.1\|29309_30107_+	sulfate ABC transporter permease	NA	NA	NA	NA
AVA38224.1\|30111_31161_+	ABC transporter ATP-binding protein	A0A2R8FG22	Brazilian_cedratvirus	1.3e-13	34.0
AVA38384.1\|31545_32508_+	hypothetical protein	NA	NA	NA	NA
AVA38225.1\|32538_33747_+	chromate transporter	NA	NA	NA	NA
AVA38226.1\|33770_34364_+	superoxide dismutase	NA	NA	NA	NA
AVA38227.1\|34381_34720_+	sulfurtransferase	NA	NA	NA	NA
AVA38228.1\|34719_35178_+	chromate resistance protein	NA	NA	NA	NA
AVA38229.1\|35284_35845_+	hypothetical protein	NA	NA	NA	NA
AVA38385.1\|36007_36334_+	hypothetical protein	NA	NA	NA	NA
AVA38230.1\|36349_36667_+	hypothetical protein	NA	NA	NA	NA
AVA38231.1\|36988_37912_+	hypothetical protein	NA	NA	NA	NA
AVA38232.1\|37963_39106_+	c-type cytochrome biogenesis protein CcsB	NA	NA	NA	NA
AVA38233.1\|39329_40346_+	hypothetical protein	NA	NA	NA	NA
AVA38234.1\|40607_41120_+	hypothetical protein	NA	NA	NA	NA
AVA38235.1\|41429_43592_-	cytochrome C biogenesis protein ResB	NA	NA	NA	NA
41621:41637	attL	NA	NA	NA	NA
AVA38386.1\|43742_45125_-	chromate transporter	A0A219VHC2	Ochrobactrum_phage	1.3e-37	77.4
AVA38236.1\|45841_46570_+	peptidase	NA	NA	NA	NA
AVA38237.1\|46836_48540_+\|integrase	integrase	NA	NA	NA	NA
AVA38238.1\|48681_52947_+	hypothetical protein	H2DE57	Erwinia_phage	1.4e-221	37.3
52101:52117	attR	NA	NA	NA	NA
AVA38239.1\|52992_53295_+	hypothetical protein	NA	NA	NA	NA
AVA38240.1\|53401_54724_+	hypothetical protein	NA	NA	NA	NA
AVA38241.1\|55148_55460_+\|transposase	transposase	NA	NA	NA	NA
AVA38242.1\|55571_57011_+\|transposase	IS481 family transposase	O12274	Simian_T-lymphotropic_virus	4.1e-05	37.8

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

3. CP026544

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

352878 : 393173

Tupanvirus(14.29%)

integrase,tRNA,protease,transposase

attL 361937:361954|attR 395750:395767

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AVA32442.1\|352878_353475_-\|tRNA	peptidyl-tRNA hydrolase	NA	NA	NA	NA
AVA32443.1\|353649_354261_-	50S ribosomal protein L25	NA	NA	NA	NA
AVA32444.1\|354431_355385_-	ribose-phosphate pyrophosphokinase	A0A2K9L2G2	Tupanvirus	2.4e-41	33.8
AVA32445.1\|355624_356503_-	4-diphosphocytidyl-2C-methyl-D-erythritol kinase	NA	NA	NA	NA
AVA32446.1\|356499_357114_-	outer membrane lipoprotein LolB	NA	NA	NA	NA
AVA37579.1\|357199_359107_-	tetratricopeptide repeat protein	NA	NA	NA	NA
AVA32447.1\|359377_360271_+	formamidopyrimidine-DNA glycosylase	NA	NA	NA	NA
AVA32448.1\|360603_362547_+	GTPase	NA	NA	NA	NA
361937:361954	attL	NA	NA	NA	NA
AVA37580.1\|362677_363829_+	A/G-specific adenine glycosylase	NA	NA	NA	NA
AVA32449.1\|363850_364504_-\|protease	ATP-dependent protease	NA	NA	NA	NA
AVA32450.1\|364550_365441_-	RNase adaptor protein RapZ	A0A1P8D5W0	Corynebacterium_phage	1.1e-08	37.4
AVA32451.1\|365555_365897_-	hypothetical protein	NA	NA	NA	NA
AVA32452.1\|366102_367074_-	HPr kinase/phosphorylase	NA	NA	NA	NA
AVA32453.1\|367142_367598_-	PTS IIA-like nitrogen-regulatory protein PtsN	NA	NA	NA	NA
AVA32454.1\|368074_368428_-	ribosome-associated translation inhibitor RaiA	NA	NA	NA	NA
AVA32455.1\|368478_369957_-	RNA polymerase sigma-54 factor	NA	NA	NA	NA
AVA32456.1\|370080_370872_-	LPS export ABC transporter ATP-binding protein	A0A2H4PQG7	Staphylococcus_phage	2.5e-20	28.0
AVA32457.1\|370897_371542_-	lipopolysaccharide transport periplasmic protein LptA	NA	NA	NA	NA
AVA32458.1\|371604_372231_-	LPS export ABC transporter periplasmic protein LptC	NA	NA	NA	NA
AVA37581.1\|372234_373218_-	KpsF/GutQ family sugar-phosphate isomerase	A0A2P0VNK5	Tetraselmis_virus	2.0e-35	33.7
AVA32459.1\|373368_375348_+	potassium transporter	NA	NA	NA	NA
AVA32460.1\|375337_375766_-	aryl-sulfate sulfotransferase	NA	NA	NA	NA
AVA32461.1\|375824_376454_-\|protease	protease	NA	NA	NA	NA
AVA32462.1\|376529_377102_+	adenine phosphoribosyltransferase	A0A1V0SKE5	Klosneuvirus	1.6e-29	44.5
AVA32463.1\|377381_379058_+	Na/Pi cotransporter family protein	NA	NA	NA	NA
AVA32464.1\|379111_381979_-	excinuclease ABC subunit UvrA	A0A1B1IVR3	uncultured_Mediterranean_phage	6.2e-303	54.8
AVA32465.1\|382253_383510_+	MFS transporter	NA	NA	NA	NA
AVA32466.1\|383785_384331_+	single-stranded DNA-binding protein	C5IHK5	Burkholderia_virus	2.3e-49	85.3
AVA32467.1\|384563_385430_+	hypothetical protein	NA	NA	NA	NA
AVA32468.1\|385617_386814_+\|integrase	site-specific integrase	NA	NA	NA	NA
AVA32469.1\|386810_388451_+	hypothetical protein	NA	NA	NA	NA
AVA32470.1\|388455_390459_+\|integrase	integrase	NA	NA	NA	NA
AVA32471.1\|390415_390838_+	hypothetical protein	NA	NA	NA	NA
AVA32472.1\|391310_391622_+\|transposase	transposase	NA	NA	NA	NA
AVA32473.1\|391733_393173_+\|transposase	IS481 family transposase	NA	NA	NA	NA
395750:395767	attR	NA	NA	NA	NA

DBSCAN-SWA_2

821840 : 833036

Ralstonia_phage(90.91%)

tRNA

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AVA32818.1\|821840_823148_+\|tRNA	serine--tRNA ligase	A0A1B1IVT2	uncultured_Mediterranean_phage	3.5e-96	52.2
AVA32819.1\|823527_824118_+	resolvase	A0JC18	Ralstonia_phage	9.1e-76	76.5
AVA37608.1\|824419_824875_-	pilus assembly protein PilA	NA	NA	NA	NA
AVA32820.1\|825088_825979_-	hypothetical protein	A0JC16	Ralstonia_phage	6.2e-52	42.1
AVA32821.1\|826673_826988_+	hypothetical protein	W6CM42	Ralstonia_phage	3.6e-31	67.4
AVA32822.1\|827182_828397_-	zonular occludens toxin	A0A097ZIG6	Ralstonia_phage	4.1e-131	59.9
AVA32823.1\|828549_828876_-	DUF2523 domain-containing protein	A0A097ZIJ0	Ralstonia_phage	7.3e-27	58.9
AVA32824.1\|828872_830336_-	hypothetical protein	A0A1W5LU35	Ralstonia_phage	9.6e-26	33.3
AVA32825.1\|830496_830718_-	hypothetical protein	A0A1W5LU14	Ralstonia_phage	2.2e-06	50.0
AVA32826.1\|830721_830961_-	hypothetical protein	NA	NA	NA	NA
AVA32827.1\|830963_831209_-	hypothetical protein	NA	NA	NA	NA
AVA32828.1\|831511_831778_-	hypothetical protein	NA	NA	NA	NA
AVA32829.1\|831830_832139_-	hypothetical protein	W6CMZ9	Ralstonia_phage	2.9e-17	50.0
AVA32830.1\|832301_832589_+	XRE family transcriptional regulator	A0A1W5LU58	Ralstonia_phage	3.4e-12	50.0
AVA32831.1\|832724_833036_+	recombinase family protein	A0JC18	Ralstonia_phage	7.2e-32	77.7

DBSCAN-SWA_3

867128 : 875971

Bacillus_phage(16.67%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AVA32859.1\|867128_868502_+	UDP-glucose/GDP-mannose dehydrogenase family protein	A0A127AXI2	Bacillus_phage	5.4e-71	35.2
AVA32860.1\|868581_869526_+	D-glycero-beta-D-manno-heptose-7-phosphate kinase	A0A2H4N7X4	Lake_Baikal_phage	3.8e-15	27.5
AVA32861.1\|869552_870548_+	ADP-L-glycero-D-mannoheptose-6-epimerase	R9S880	Prochlorococcus_phage	3.1e-28	33.4
AVA32862.1\|870757_871138_+	helix-hairpin-helix domain-containing protein	NA	NA	NA	NA
AVA32863.1\|871235_872684_+	pyrimidine utilization transport protein G	Q9KX94	Enterobacteria_phage	1.3e-144	64.4
AVA32864.1\|872794_873697_+	cysteine synthase CysM	C3U2M1	Lactococcus_phage	6.3e-52	41.2
AVA37610.1\|873776_874883_-	lytic murein transglycosylase B	NA	NA	NA	NA
AVA32865.1\|875047_875971_+	deacetylase	A0A2K9KZC4	Tupanvirus	8.7e-41	34.0

DBSCAN-SWA_4

998285 : 1006734

Pseudomonas_virus(28.57%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AVA32975.1\|998285_998507_-	transcriptional regulator	A0A077K9Z8	Ralstonia_phage	1.9e-15	68.4
AVA32976.1\|998566_998773_-	hypothetical protein	NA	NA	NA	NA
AVA32977.1\|998769_999201_-	hypothetical protein	G1FTZ6	Mycobacterium_phage	3.1e-17	42.6
AVA32978.1\|999214_999859_-	hypothetical protein	Q5QF30	Pseudomonas_virus	3.4e-36	44.3
AVA32979.1\|1000140_1000365_+	hypothetical protein	NA	NA	NA	NA
AVA32980.1\|1000376_1002161_-	DNA cytosine methyltransferase	L7TH64	Pseudomonas_virus	2.1e-176	61.1
AVA32981.1\|1002157_1002766_-	hypothetical protein	NA	NA	NA	NA
AVA32982.1\|1002762_1003176_-	hypothetical protein	NA	NA	NA	NA
AVA32983.1\|1003177_1004050_-	hypothetical protein	A0A067XQE4	Caulobacter_phage	1.0e-27	32.7
AVA32984.1\|1004239_1004887_-	hypothetical protein	NA	NA	NA	NA
AVA32985.1\|1004914_1005106_-	hypothetical protein	A9YX20	Burkholderia_phage	5.2e-09	50.0
AVA32986.1\|1005102_1006734_-	endonuclease	A0A2H4J6P1	uncultured_Caudovirales_phage	5.0e-92	46.0

DBSCAN-SWA_5

1010091 : 1042091

Aeromonas_phage(17.24%)

integrase,head,terminase

attL 1033568:1033583|attR 1047379:1047394

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AVA32993.1\|1010091_1010850_-	hypothetical protein	A0A0U4JEE2	Pseudomonas_phage	1.4e-36	49.0
AVA32994.1\|1011158_1011347_+	hypothetical protein	NA	NA	NA	NA
AVA32995.1\|1011336_1011528_+	hypothetical protein	NA	NA	NA	NA
AVA32996.1\|1011599_1011932_-	hypothetical protein	NA	NA	NA	NA
AVA32997.1\|1012009_1012387_+	hypothetical protein	NA	NA	NA	NA
AVA37617.1\|1012425_1013448_+	site-specific DNA-methyltransferase	Q8W6P4	Burkholderia_virus	5.9e-115	59.4
AVA32998.1\|1013444_1014518_+	hypothetical protein	H2BD69	Pseudomonas_phage	6.3e-35	46.6
AVA32999.1\|1014521_1015268_+	hypothetical protein	U6C6D0	Ralstonia_phage	3.0e-31	37.7
AVA33000.1\|1015452_1015890_+	hypothetical protein	NA	NA	NA	NA
AVA37618.1\|1015904_1016435_+	RusA family crossover junction endodeoxyribonuclease	NA	NA	NA	NA
AVA33001.1\|1016827_1017151_+	hypothetical protein	A0A0S4L5D5	Pseudomonas_phage	4.2e-35	75.2
AVA33002.1\|1017189_1017618_+	hypothetical protein	A0A1W6DY69	Salmonella_phage	8.4e-23	43.6
AVA33003.1\|1017753_1019217_+\|terminase	PBSX family phage terminase large subunit	A0A1W6DY05	Salmonella_phage	2.0e-116	43.4
AVA33004.1\|1019266_1019752_+	hypothetical protein	NA	NA	NA	NA
AVA33005.1\|1019768_1021382_+	hypothetical protein	A0A2R3UAL5	Myoviridae_environmental_samples	1.9e-75	36.9
AVA33006.1\|1021381_1022191_+\|head	phage head morphogenesis protein	H9C0V1	Aeromonas_phage	1.8e-50	45.8
AVA37619.1\|1022177_1023410_+	hypothetical protein	A0A2R3UAL3	Myoviridae_environmental_samples	2.9e-47	33.0
AVA33007.1\|1023422_1023899_+	hypothetical protein	A0A077KAW3	Edwardsiella_phage	2.2e-19	40.5
AVA33008.1\|1023895_1024906_+	DUF2184 domain-containing protein	Q8HAP7	Burkholderia_phage	9.8e-70	42.1
AVA33009.1\|1024907_1025309_+	hypothetical protein	NA	NA	NA	NA
AVA33010.1\|1025312_1025723_+	DUF4054 domain-containing protein	NA	NA	NA	NA
AVA33011.1\|1025709_1026189_+	hypothetical protein	A0A190XCA2	Acinetobacter_phage	4.1e-18	38.4
AVA33012.1\|1026185_1026581_+	hypothetical protein	A0A077KAW7	Edwardsiella_phage	1.7e-17	37.4
AVA33013.1\|1026583_1027129_+	hypothetical protein	A6N3B1	Burkholderia_virus	1.3e-07	25.4
AVA33014.1\|1027132_1028854_+	hypothetical protein	A0A077KGV4	Edwardsiella_phage	3.5e-35	31.6
AVA33015.1\|1028866_1029301_+	hypothetical protein	H9C0W6	Aeromonas_phage	6.5e-31	46.5
AVA33016.1\|1029302_1029743_+	hypothetical protein	H9C0W7	Aeromonas_phage	5.8e-19	48.0
AVA33017.1\|1029739_1029910_+	transcription elongation factor GreA	NA	NA	NA	NA
AVA33018.1\|1029918_1031907_+	hypothetical protein	U5PVY0	Bacillus_phage	7.2e-16	41.3
AVA33019.1\|1031908_1032559_+	hypothetical protein	A0A1X9SFH6	Acinetobacter_phage	6.8e-24	39.7
AVA33020.1\|1032558_1032858_+	hypothetical protein	K4I3B0	Acinetobacter_phage	2.0e-07	38.4
AVA33021.1\|1032854_1033724_+	hypothetical protein	NA	NA	NA	NA
1033568:1033583	attL	NA	NA	NA	NA
AVA33022.1\|1033716_1034484_+	oxidoreductase	A0A2R3UAK1	Myoviridae_environmental_samples	1.8e-36	45.7
AVA33023.1\|1034490_1034844_+	hypothetical protein	A0A2R3UAP1	Myoviridae_environmental_samples	1.1e-15	46.0
AVA33024.1\|1034852_1036085_+	hypothetical protein	H9C0X9	Aeromonas_phage	4.2e-75	41.1
AVA33025.1\|1036084_1036741_+	DUF2612 domain-containing protein	H9C0Y0	Aeromonas_phage	2.9e-46	49.3
AVA33026.1\|1036754_1038125_+	hypothetical protein	Q8HAM8	Burkholderia_phage	3.6e-51	34.8
AVA33027.1\|1038133_1038706_+	hypothetical protein	NA	NA	NA	NA
AVA33028.1\|1038723_1039218_+	lysozyme	D5LH07	Escherichia_phage	4.6e-57	71.1
AVA33029.1\|1039220_1039523_+	hypothetical protein	NA	NA	NA	NA
AVA33030.1\|1039526_1039790_+	hypothetical protein	NA	NA	NA	NA
AVA33031.1\|1039786_1040302_+	hypothetical protein	U6C7Z8	Ralstonia_phage	2.6e-18	42.2
AVA33032.1\|1040305_1040791_+	hypothetical protein	NA	NA	NA	NA
AVA33033.1\|1040837_1042091_-\|integrase	integrase	A0A077KET4	Ralstonia_phage	6.7e-153	64.2
1047379:1047394	attR	NA	NA	NA	NA

DBSCAN-SWA_6

1767824 : 1788945

Wolbachia_phage(25.0%)

integrase,transposase

attL 1758371:1758384|attR 1777887:1777900

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
1758371:1758384	attL	NA	NA	NA	NA
AVA33661.1\|1767824_1769828_+\|integrase	integrase	NA	NA	NA	NA
AVA33662.1\|1769857_1770835_+	ATP-binding protein	NA	NA	NA	NA
AVA37669.1\|1770902_1771454_+	hypothetical protein	NA	NA	NA	NA
AVA33663.1\|1771627_1771840_+	hypothetical protein	NA	NA	NA	NA
AVA33664.1\|1772213_1772435_-	hypothetical protein	NA	NA	NA	NA
AVA33665.1\|1772862_1774536_-\|integrase	integrase	NA	NA	NA	NA
AVA33666.1\|1774834_1775275_+	Hsp20/alpha crystallin family protein	NA	NA	NA	NA
AVA33667.1\|1775290_1775698_+	heat-shock protein	A0A1B2LRT2	Wolbachia_phage	1.5e-05	29.2
AVA33668.1\|1775893_1776463_+	Hsp20/alpha crystallin family protein	NA	NA	NA	NA
AVA33669.1\|1776582_1776972_+	hypothetical protein	NA	NA	NA	NA
AVA33670.1\|1777113_1779054_+	molecular chaperone DnaK	A0A0N9QXN8	Chrysochromulina_ericina_virus	2.1e-145	49.0
1777887:1777900	attR	NA	NA	NA	NA
AVA33671.1\|1779145_1779418_+	hypothetical protein	NA	NA	NA	NA
AVA37670.1\|1779745_1780312_+	Hsp20/alpha crystallin family protein	NA	NA	NA	NA
AVA33672.1\|1781492_1782365_+	hypothetical protein	NA	NA	NA	NA
AVA33673.1\|1782392_1783578_+\|transposase	IS3-like element ISRme13 family transposase	A0A0P0I4A4	Acinetobacter_phage	3.2e-56	39.7
AVA33674.1\|1784989_1786200_-\|transposase	IS3 family transposase	A0A1B0Z042	Pseudomonas_phage	1.1e-99	62.7
AVA33675.1\|1787082_1787394_+\|transposase	transposase	NA	NA	NA	NA
AVA33676.1\|1787505_1788945_+\|transposase	IS481 family transposase	NA	NA	NA	NA

DBSCAN-SWA_7

1807213 : 1848635

Burkholderia_phage(33.33%)

integrase,transposase

attL 1826287:1826303|attR 1837765:1837781

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AVA33693.1\|1807213_1808887_-\|integrase	integrase	NA	NA	NA	NA
AVA33694.1\|1809162_1809381_+	hypothetical protein	NA	NA	NA	NA
AVA33695.1\|1809488_1809692_+	hypothetical protein	NA	NA	NA	NA
AVA33696.1\|1809751_1810219_-	hypothetical protein	NA	NA	NA	NA
AVA33697.1\|1810930_1811131_-	hypothetical protein	NA	NA	NA	NA
AVA33698.1\|1811649_1812636_+\|transposase	IS5/IS1182 family transposase	E5E3P6	Burkholderia_phage	4.2e-150	82.3
AVA37673.1\|1813414_1814233_-	copper resistance protein CopB	NA	NA	NA	NA
AVA33699.1\|1816508_1816835_+	four-helix bundle copper-binding protein	NA	NA	NA	NA
AVA33700.1\|1818900_1819197_-	hypothetical protein	NA	NA	NA	NA
AVA33701.1\|1820267_1820516_-	hypothetical protein	NA	NA	NA	NA
AVA33702.1\|1821060_1823172_+	TonB-dependent siderophore receptor	NA	NA	NA	NA
AVA33703.1\|1823540_1823786_+	hypothetical protein	NA	NA	NA	NA
AVA33704.1\|1824015_1824225_-	hypothetical protein	NA	NA	NA	NA
AVA33705.1\|1824497_1824920_+	flagellar basal body rod protein FlgB	NA	NA	NA	NA
1826287:1826303	attL	NA	NA	NA	NA
AVA37674.1\|1826598_1827378_+	methyltransferase	NA	NA	NA	NA
AVA33706.1\|1827436_1827637_+	hypothetical protein	NA	NA	NA	NA
AVA33707.1\|1828184_1828580_+	phosphoesterase	NA	NA	NA	NA
AVA33708.1\|1828759_1829629_+	methyltransferase domain-containing protein	NA	NA	NA	NA
AVA33709.1\|1829625_1831251_+	glycosyl transferase	NA	NA	NA	NA
AVA33710.1\|1831247_1831631_+	GtrA family protein	NA	NA	NA	NA
AVA33711.1\|1831627_1832716_+	glycosyl transferase family 2	U5P087	Shigella_phage	9.2e-74	47.5
AVA33712.1\|1832952_1834020_+	porin	NA	NA	NA	NA
AVA37675.1\|1835132_1835777_-\|integrase	integrase	NA	NA	NA	NA
AVA33713.1\|1836265_1837672_-	TolC family protein	NA	NA	NA	NA
AVA33714.1\|1839978_1840353_+	NUDIX hydrolase	NA	NA	NA	NA
1837765:1837781	attR	NA	NA	NA	NA
AVA33715.1\|1840856_1841591_+	hypothetical protein	NA	NA	NA	NA
AVA33716.1\|1841953_1842163_-	hypothetical protein	NA	NA	NA	NA
AVA33717.1\|1842708_1843170_+	CopH protein	NA	NA	NA	NA
AVA33718.1\|1844042_1845229_-\|transposase	IS3-like element ISRme13 family transposase	A0A0P0I4A4	Acinetobacter_phage	3.2e-56	39.7
AVA33719.1\|1846146_1846413_+	hypothetical protein	NA	NA	NA	NA
AVA33720.1\|1846772_1847084_+\|transposase	transposase	NA	NA	NA	NA
AVA33721.1\|1847195_1848635_+\|transposase	IS481 family transposase	NA	NA	NA	NA

DBSCAN-SWA_8

1874995 : 1938218

Bacillus_phage(27.27%)

integrase,transposase

attL 1912218:1912255|attR 1938223:1938260

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AVA33743.1\|1874995_1877962_+\|transposase	Tn3 family transposase	A0A1B0V7H9	Salmonella_phage	0.0e+00	72.9
AVA33744.1\|1878313_1878601_+	nickel-cobalt-cadmium resistance protein NccY	NA	NA	NA	NA
AVA33745.1\|1878597_1879044_+	nickel-cobalt-cadmium resistance protein NccX	NA	NA	NA	NA
AVA37677.1\|1879115_1879601_+	RNA polymerase subunit sigma	NA	NA	NA	NA
AVA33746.1\|1879791_1881048_+	TolC family protein	NA	NA	NA	NA
AVA33747.1\|1881044_1882241_+	efflux transporter periplasmic adaptor subunit	NA	NA	NA	NA
AVA33748.1\|1882237_1885468_+	CusA/CzcA family heavy metal efflux RND transporter	NA	NA	NA	NA
AVA33749.1\|1885538_1886180_+	isoprenylcysteine carboxylmethyltransferase family protein	NA	NA	NA	NA
AVA33750.1\|1886199_1886877_+	DNA-binding response regulator	NA	NA	NA	NA
AVA33751.1\|1886878_1887316_+	hypothetical protein	NA	NA	NA	NA
AVA33752.1\|1887380_1888106_+	hypothetical protein	NA	NA	NA	NA
AVA33753.1\|1888116_1888887_+	hypothetical protein	NA	NA	NA	NA
AVA33754.1\|1889144_1889414_+	nickel resistance protein	NA	NA	NA	NA
AVA33755.1\|1889501_1890842_+	MFS transporter	NA	NA	NA	NA
AVA33756.1\|1891114_1891294_-	hypothetical protein	NA	NA	NA	NA
AVA33757.1\|1891309_1891660_+	flagellar basal body rod protein FlgB	NA	NA	NA	NA
AVA33758.1\|1891882_1893484_+	glycosyltransferase family 39 protein	NA	NA	NA	NA
AVA33759.1\|1893480_1893864_+	GtrA family protein	NA	NA	NA	NA
AVA37678.1\|1893875_1894964_+	glycosyltransferase	U5P087	Shigella_phage	3.0e-72	47.7
AVA33760.1\|1895109_1896342_+	RimK family alpha-L-glutamate ligase	NA	NA	NA	NA
AVA33761.1\|1896383_1897418_+	taurine ABC transporter substrate-binding protein	NA	NA	NA	NA
AVA33762.1\|1897424_1898252_+	ABC transporter ATP-binding protein	G3M9Y6	Bacillus_virus	6.8e-29	36.4
AVA33763.1\|1898238_1899102_+	taurine ABC transporter permease	NA	NA	NA	NA
AVA33764.1\|1899147_1899840_+	VIT family protein	NA	NA	NA	NA
AVA33765.1\|1900021_1901221_-	chromate transporter	NA	NA	NA	NA
AVA33766.1\|1902387_1902765_-	hypothetical protein	NA	NA	NA	NA
AVA33767.1\|1902838_1905943_-	CusA/CzcA family heavy metal efflux RND transporter	S5VTK5	Leptospira_phage	1.2e-62	23.5
AVA33768.1\|1905952_1907065_-	hypothetical protein	NA	NA	NA	NA
AVA33769.1\|1907634_1908840_-	transporter	NA	NA	NA	NA
AVA33770.1\|1908933_1909296_-	hypothetical protein	NA	NA	NA	NA
AVA33771.1\|1909445_1910528_-	porin	NA	NA	NA	NA
AVA33772.1\|1910594_1911780_+\|transposase	IS3-like element ISRme13 family transposase	A0A0P0I4A4	Acinetobacter_phage	3.2e-56	39.7
AVA33773.1\|1912001_1912217_-	hypothetical protein	NA	NA	NA	NA
1912218:1912255	attL	NA	NA	NA	NA
AVA33774.1\|1912265_1915259_-\|transposase	Tn3 family transposase	NA	NA	NA	NA
AVA33775.1\|1915301_1916612_-	Tn3 family resolvase	NA	NA	NA	NA
AVA33776.1\|1916917_1917538_-	superoxide dismutase	NA	NA	NA	NA
AVA33777.1\|1917537_1917885_-	hypothetical protein	NA	NA	NA	NA
AVA37679.1\|1918034_1919210_-	chromate transporter	NA	NA	NA	NA
AVA33778.1\|1919601_1919871_+	type II toxin-antitoxin system antitoxin, RelB/DinJ family	NA	NA	NA	NA
AVA33779.1\|1919857_1920139_+	type II toxin-antitoxin system YafQ family toxin	NA	NA	NA	NA
AVA33780.1\|1920354_1920927_+	recombinase family protein	A0A286S1P7	Klebsiella_phage	1.2e-37	51.4
AVA33781.1\|1920923_1921307_+	hypothetical protein	NA	NA	NA	NA
AVA33782.1\|1921354_1922086_-	hypothetical protein	NA	NA	NA	NA
AVA33783.1\|1922084_1922354_+	hypothetical protein	NA	NA	NA	NA
AVA33784.1\|1922396_1923179_+	TIGR02391 family protein	A0A1S5SBU1	Streptococcus_phage	1.6e-35	39.6
AVA33785.1\|1923122_1924193_-	aminoglycoside phosphotransferase family protein	NA	NA	NA	NA
AVA33786.1\|1924208_1926011_-	ABC transporter ATP-binding protein	W8CYL7	Bacillus_phage	5.9e-33	26.3
AVA33787.1\|1926007_1927834_-	ABC transporter ATP-binding protein	W8CYL7	Bacillus_phage	1.0e-24	24.7
AVA33788.1\|1927880_1928240_-	hypothetical protein	NA	NA	NA	NA
AVA33789.1\|1928299_1929202_-	DUF1259 domain-containing protein	NA	NA	NA	NA
AVA33790.1\|1929274_1929664_-	hypothetical protein	NA	NA	NA	NA
AVA33791.1\|1929679_1930138_-	chromate resistance protein	NA	NA	NA	NA
AVA33792.1\|1930142_1931051_-	superoxide dismutase	Q56AR7	Bacillus_thuringiensis_phage	1.0e-09	35.5
AVA33793.1\|1931112_1932309_-	chromate transporter	NA	NA	NA	NA
AVA33794.1\|1932348_1933311_-	hypothetical protein	NA	NA	NA	NA
AVA33795.1\|1933542_1935081_-\|transposase	IS3 family transposase	A0A1B1P773	Bacillus_phage	1.2e-47	37.5
AVA37680.1\|1935379_1936402_-\|transposase	IS110 family transposase	NA	NA	NA	NA
AVA33796.1\|1937120_1938218_+\|integrase	integrase	NA	NA	NA	NA
1938223:1938260	attR	NA	NA	NA	NA

DBSCAN-SWA_9

1975337 : 2031395

Salmonella_phage(40.0%)

integrase,transposase

attL 2022802:2022817|attR 2038696:2038711

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AVA33827.1\|1975337_1977017_-\|integrase	integrase	NA	NA	NA	NA
AVA33828.1\|1977809_1978955_+	PAS domain S-box protein	NA	NA	NA	NA
AVA33829.1\|1978951_1979596_+	DNA-binding response regulator	NA	NA	NA	NA
AVA33830.1\|1979626_1980079_-	universal stress protein	NA	NA	NA	NA
AVA33831.1\|1980299_1983266_-\|transposase	Tn3 family transposase	A0A1B0V7H9	Salmonella_phage	0.0e+00	72.9
AVA37681.1\|1983268_1983838_-	recombinase family protein	A0A1B0V7I5	Salmonella_phage	2.2e-50	88.6
AVA33832.1\|1983743_1984745_-	DUF3330 domain-containing protein	NA	NA	NA	NA
AVA33833.1\|1984741_1984978_-	mercury resistance protein	NA	NA	NA	NA
AVA33834.1\|1984974_1985340_-	mercuric resistance transcriptional repressor protein MerD	NA	NA	NA	NA
AVA33835.1\|1985357_1987043_-	mercury(II) reductase	A0A2K5B2C5	Erysipelothrix_phage	2.1e-40	29.1
AVA33836.1\|1987114_1987390_-	mercuric transport protein periplasmic component	NA	NA	NA	NA
AVA33837.1\|1987402_1987753_-	mercuric transporter	NA	NA	NA	NA
AVA33838.1\|1987824_1988259_+	mercuric resistance operon regulatory protein	NA	NA	NA	NA
AVA33839.1\|1989776_1991894_-	PAS domain S-box protein	G3MA91	Bacillus_virus	1.5e-24	40.1
AVA33840.1\|1991903_1992239_-	hypothetical protein	NA	NA	NA	NA
AVA33841.1\|1993923_1994346_-	flagellar basal body rod protein FlgB	NA	NA	NA	NA
AVA33842.1\|1994617_1994863_+	hypothetical protein	NA	NA	NA	NA
AVA33843.1\|1998307_1998541_+	hypothetical protein	NA	NA	NA	NA
AVA33844.1\|1998885_1999278_+	hypothetical protein	A4PE56	Ralstonia_virus	1.5e-10	38.6
AVA33845.1\|1999643_2000879_+	polyhydroxyalkanoate depolymerase	NA	NA	NA	NA
AVA33846.1\|2001821_2002109_+	hypothetical protein	NA	NA	NA	NA
AVA37682.1\|2003649_2003859_+	hypothetical protein	NA	NA	NA	NA
AVA33847.1\|2004983_2005388_+	hypothetical protein	NA	NA	NA	NA
AVA33848.1\|2005457_2006759_+	hypothetical protein	NA	NA	NA	NA
AVA33849.1\|2006755_2008291_+	efflux RND transporter periplasmic adaptor subunit	NA	NA	NA	NA
AVA33850.1\|2008287_2011458_+	CusA/CzcA family heavy metal efflux RND transporter	NA	NA	NA	NA
AVA33851.1\|2011454_2011829_+	hypothetical protein	NA	NA	NA	NA
AVA33852.1\|2011890_2012076_+	hypothetical protein	NA	NA	NA	NA
AVA33853.1\|2012182_2012362_-	hypothetical protein	NA	NA	NA	NA
AVA33854.1\|2013278_2015396_+	TonB-dependent siderophore receptor	NA	NA	NA	NA
AVA33855.1\|2017011_2018064_-\|transposase	IS630 family transposase	NA	NA	NA	NA
AVA33856.1\|2018111_2018594_-	glyoxalase/bleomycin resistance/dioxygenase family protein	NA	NA	NA	NA
AVA37683.1\|2019246_2019525_+	transcriptional regulator	NA	NA	NA	NA
AVA37684.1\|2019566_2020574_+	cation transporter	NA	NA	NA	NA
AVA33857.1\|2022140_2022452_+\|transposase	transposase	NA	NA	NA	NA
AVA33858.1\|2022563_2024003_+\|transposase	IS481 family transposase	NA	NA	NA	NA
2022802:2022817	attL	NA	NA	NA	NA
AVA33859.1\|2023999_2024833_+	general secretion pathway protein GspA	NA	NA	NA	NA
AVA33860.1\|2024829_2025102_+	hypothetical protein	NA	NA	NA	NA
AVA33861.1\|2029709_2031395_-\|integrase	integrase	NA	NA	NA	NA
2038696:2038711	attR	NA	NA	NA	NA

DBSCAN-SWA_10

2648650 : 2762905

Bacillus_phage(15.79%)

integrase,transposase

attL 2647484:2647501|attR 2684317:2684334

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
2647484:2647501	attL	NA	NA	NA	NA
AVA34376.1\|2648650_2650090_-\|transposase	IS481 family transposase	NA	NA	NA	NA
AVA34377.1\|2650201_2650513_-\|transposase	transposase	NA	NA	NA	NA
AVA34378.1\|2650859_2652011_-	hypothetical protein	NA	NA	NA	NA
AVA34379.1\|2652486_2653032_+	3-keto-5-aminohexanoate cleavage protein	NA	NA	NA	NA
AVA34380.1\|2653044_2653581_-	nitronate monooxygenase	NA	NA	NA	NA
AVA34381.1\|2653605_2654202_-	hypothetical protein	NA	NA	NA	NA
AVA34382.1\|2654712_2655078_+	hypothetical protein	NA	NA	NA	NA
AVA34383.1\|2655400_2655637_+	antitoxin VapB	NA	NA	NA	NA
AVA34384.1\|2655623_2655836_+	hypothetical protein	NA	NA	NA	NA
AVA34385.1\|2655846_2656062_+	hypothetical protein	NA	NA	NA	NA
AVA34386.1\|2656379_2656661_-	hypothetical protein	NA	NA	NA	NA
AVA34387.1\|2656983_2657175_+	hypothetical protein	NA	NA	NA	NA
AVA34388.1\|2657248_2657875_+\|integrase	integrase	A0A0A8WI70	Clostridium_phage	2.5e-07	31.5
AVA34389.1\|2658306_2658951_-	acyl-CoA dehydrogenase	NA	NA	NA	NA
AVA34390.1\|2659120_2659387_-	hypothetical protein	NA	NA	NA	NA
AVA34391.1\|2659581_2659935_-	copper resistance protein CopV	NA	NA	NA	NA
AVA37728.1\|2660002_2660767_-	cytochrome c	NA	NA	NA	NA
AVA34392.1\|2662073_2662358_+	copper resistance protein K	NA	NA	NA	NA
AVA34393.1\|2662382_2662835_-	copper resistance protein CopN	NA	NA	NA	NA
AVA34394.1\|2662927_2664319_-	two-component sensor histidine kinase	A0A1V0SGX0	Hokovirus	3.3e-07	26.9
AVA34395.1\|2664315_2665002_-	DNA-binding response regulator	W8CYM9	Bacillus_phage	4.6e-31	33.9
AVA34396.1\|2665205_2667050_+	copper resistance system multicopper oxidase	NA	NA	NA	NA
AVA37729.1\|2667621_2668569_+	copper resistance protein B	NA	NA	NA	NA
AVA34397.1\|2668612_2668999_+	copper resistance protein CopC	NA	NA	NA	NA
AVA34398.1\|2669005_2669923_+	copper resistance protein CopD	NA	NA	NA	NA
AVA34399.1\|2670194_2670629_-	mercuric resistance operon regulatory protein	NA	NA	NA	NA
AVA34400.1\|2670700_2671051_+	mercuric transporter	NA	NA	NA	NA
AVA34401.1\|2671063_2671339_+	mercuric transport protein periplasmic component	NA	NA	NA	NA
AVA34402.1\|2671410_2673096_+	mercury(II) reductase	A0A2K5B2C5	Erysipelothrix_phage	2.1e-40	29.1
AVA34403.1\|2673113_2673479_+	mercuric resistance transcriptional repressor protein MerD	NA	NA	NA	NA
AVA34404.1\|2673475_2673712_+	mercury resistance protein	NA	NA	NA	NA
AVA34405.1\|2673708_2674710_+	DUF3330 domain-containing protein	NA	NA	NA	NA
AVA37730.1\|2674615_2675185_+	recombinase family protein	A0A1B0V7I5	Salmonella_phage	2.2e-50	88.6
AVA34406.1\|2675187_2678154_+\|transposase	Tn3 family transposase	A0A1B0V7H9	Salmonella_phage	0.0e+00	72.9
AVA34407.1\|2678766_2679942_+\|integrase	integrase	A0A166YH27	Gordonia_phage	1.4e-06	27.8
AVA34408.1\|2679981_2683308_+	helicase	A0A0N7D8L3	Dasychira_pudibunda_nucleopolyhedrovirus	1.7e-54	32.5
AVA34409.1\|2683877_2684648_-	hypothetical protein	NA	NA	NA	NA
2684317:2684334	attR	NA	NA	NA	NA
AVA34410.1\|2684644_2685583_-	transcriptional regulator	NA	NA	NA	NA
AVA34411.1\|2686961_2687420_-	MgtC/SapB family protein	G3MA03	Bacillus_virus	3.2e-12	36.2
AVA34412.1\|2687603_2688320_+	isoprenylcysteine carboxylmethyltransferase family protein	NA	NA	NA	NA
AVA34413.1\|2688349_2688529_+	hypothetical protein	NA	NA	NA	NA
AVA34414.1\|2688592_2688916_-	hypothetical protein	NA	NA	NA	NA
AVA34415.1\|2689057_2690314_+	cobalt-zinc-cadmium resistance protein CzcC	NA	NA	NA	NA
AVA34416.1\|2690331_2691894_+	cobalt-zinc-cadmium resistance protein CzcB	NA	NA	NA	NA
AVA34417.1\|2691910_2695102_+	cation efflux system protein CzcA	NA	NA	NA	NA
AVA34418.1\|2695152_2696103_+	metal cation efflux system protein CzcD	NA	NA	NA	NA
AVA34419.1\|2696104_2696782_+	DNA-binding response regulator	W8CYM9	Bacillus_phage	4.9e-33	33.8
AVA34420.1\|2696753_2698217_+	sensor histidine kinase	W8CYF6	Bacillus_phage	1.9e-13	21.9
AVA34421.1\|2698285_2699471_+\|transposase	IS3-like element ISRme13 family transposase	A0A0P0I4A4	Acinetobacter_phage	3.2e-56	39.7
AVA34422.1\|2699620_2700688_-	porin	NA	NA	NA	NA
AVA34423.1\|2700804_2701991_-\|transposase	IS3-like element ISRme13 family transposase	A0A0P0I4A4	Acinetobacter_phage	3.2e-56	39.7
AVA34424.1\|2702275_2702698_+	flagellar basal body rod protein FlgB	NA	NA	NA	NA
AVA34425.1\|2703197_2705687_+	heavy metal translocating P-type ATPase	E4ZFI9	Streptococcus_phage	3.9e-112	39.0
AVA34426.1\|2705744_2706047_-	zinc ribbon domain-containing protein	NA	NA	NA	NA
AVA34427.1\|2706275_2707397_-	glycosyltransferase	U5P087	Shigella_phage	1.6e-73	48.3
AVA34428.1\|2707393_2707777_-	GtrA family protein	NA	NA	NA	NA
AVA34429.1\|2707773_2709381_-	glycosyl transferase	NA	NA	NA	NA
AVA34430.1\|2709481_2709706_+	hypothetical protein	NA	NA	NA	NA
AVA34431.1\|2710815_2711061_+	hypothetical protein	NA	NA	NA	NA
AVA34432.1\|2711780_2713418_+	glycosyl transferase	NA	NA	NA	NA
AVA34433.1\|2713414_2713798_+	GtrA family protein	NA	NA	NA	NA
AVA34434.1\|2713794_2714916_+	glycosyltransferase	U5P087	Shigella_phage	1.6e-73	48.3
AVA34435.1\|2714961_2715483_-	class I SAM-dependent methyltransferase	NA	NA	NA	NA
AVA34436.1\|2716280_2716505_-	hypothetical protein	NA	NA	NA	NA
AVA34437.1\|2717027_2717306_+	hypothetical protein	NA	NA	NA	NA
AVA34438.1\|2717900_2718122_+	hypothetical protein	NA	NA	NA	NA
AVA34439.1\|2718283_2718682_+	copper resistance protein	NA	NA	NA	NA
AVA34440.1\|2718754_2719756_+	hypothetical protein	NA	NA	NA	NA
AVA34441.1\|2719842_2720115_-	hypothetical protein	NA	NA	NA	NA
AVA34442.1\|2720111_2720945_-	general secretion pathway protein GspA	NA	NA	NA	NA
AVA34443.1\|2720941_2722381_-\|transposase	IS481 family transposase	NA	NA	NA	NA
AVA34444.1\|2722492_2722804_-\|transposase	transposase	NA	NA	NA	NA
AVA34445.1\|2722737_2723583_+	hypothetical protein	NA	NA	NA	NA
AVA34446.1\|2723579_2725142_+	efflux RND transporter periplasmic adaptor subunit	NA	NA	NA	NA
AVA34447.1\|2725138_2728309_+	CusA/CzcA family heavy metal efflux RND transporter	NA	NA	NA	NA
AVA34448.1\|2728305_2728644_+	hypothetical protein	NA	NA	NA	NA
AVA34449.1\|2729132_2729780_-	cytochrome c	NA	NA	NA	NA
AVA34450.1\|2729787_2730012_-	hypothetical protein	NA	NA	NA	NA
AVA34451.1\|2730323_2730650_-	four-helix bundle copper-binding protein	NA	NA	NA	NA
AVA37731.1\|2732075_2732444_+	IS66 family insertion sequence hypothetical protein	NA	NA	NA	NA
AVA34452.1\|2732419_2732635_+	IS66 family insertion sequence hypothetical protein	NA	NA	NA	NA
AVA34453.1\|2732708_2732912_+	hypothetical protein	NA	NA	NA	NA
AVA34454.1\|2734405_2735416_-\|transposase	IS30 family transposase ISRme10	Q9MBM9	Staphylococcus_prophage	1.2e-40	34.3
AVA34455.1\|2735500_2735764_+	hypothetical protein	NA	NA	NA	NA
AVA37732.1\|2739611_2739917_-	hypothetical protein	NA	NA	NA	NA
AVA34456.1\|2740369_2740570_+	hypothetical protein	NA	NA	NA	NA
AVA34457.1\|2740644_2741721_-	signal peptidase II	NA	NA	NA	NA
AVA34458.1\|2741717_2744117_-	copper-translocating P-type ATPase	E4ZFI9	Streptococcus_phage	6.2e-147	48.1
AVA34459.1\|2744203_2744641_+	Cd(II)/Pb(II)-responsive transcriptional regulator	NA	NA	NA	NA
AVA34460.1\|2755242_2756334_+	signal peptidase II	NA	NA	NA	NA
AVA37733.1\|2756382_2758299_+	iron permease	NA	NA	NA	NA
AVA34461.1\|2758386_2759001_-	recombinase family protein	A0A0A7NPV4	Enterobacteria_phage	2.8e-35	49.0
AVA34462.1\|2759061_2760279_-\|transposase	transposase	NA	NA	NA	NA
AVA34463.1\|2760275_2761184_-\|transposase	transposase	NA	NA	NA	NA
AVA34464.1\|2761186_2762905_-\|transposase	transposase	NA	NA	NA	NA

DBSCAN-SWA_11

4476083 : 4527127

Salmonella_phage(28.57%)

integrase,transposase

attL 4499305:4499321|attR 4527537:4527553

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AVA35853.1\|4476083_4479050_+\|transposase	Tn3 family transposase	A0A1B0V7H9	Salmonella_phage	0.0e+00	72.9
AVA35854.1\|4479770_4481408_-	sigma-54-dependent Fis family transcriptional regulator	NA	NA	NA	NA
AVA35855.1\|4481670_4482258_+	peroxiredoxin	NA	NA	NA	NA
AVA35856.1\|4482303_4483446_+	acyl-CoA dehydrogenase	NA	NA	NA	NA
AVA35857.1\|4483442_4484789_+	rubredoxin	NA	NA	NA	NA
AVA35858.1\|4484847_4486245_+	nitrate ABC transporter substrate-binding protein	NA	NA	NA	NA
AVA35859.1\|4486264_4487149_+	nitrate ABC transporter, permease protein	NA	NA	NA	NA
AVA35860.1\|4487138_4488089_+	nitrate/sulfonate/bicarbonate ABC transporter ATP-binding protein	G3M9Y6	Bacillus_virus	9.9e-32	39.2
AVA35861.1\|4488136_4488586_+	cyanase	NA	NA	NA	NA
AVA35862.1\|4488627_4489428_+	sulfite exporter TauE/SafE family protein	NA	NA	NA	NA
AVA35863.1\|4489441_4490305_+	MBL fold metallo-hydrolase	NA	NA	NA	NA
AVA35864.1\|4490959_4491382_-	DNA-binding protein	NA	NA	NA	NA
AVA35865.1\|4491465_4492524_-	hypothetical protein	NA	NA	NA	NA
AVA35866.1\|4492563_4493001_-	hypothetical protein	NA	NA	NA	NA
AVA37863.1\|4493185_4493875_-\|integrase	site-specific integrase	NA	NA	NA	NA
AVA35867.1\|4497861_4498422_-\|transposase	transposase	A0A1B0V7I5	Salmonella_phage	1.6e-50	87.1
AVA35868.1\|4498834_4501774_+	DUF4158 domain-containing protein	NA	NA	NA	NA
4499305:4499321	attL	NA	NA	NA	NA
AVA35869.1\|4501770_4502658_+	hypothetical protein	NA	NA	NA	NA
AVA35870.1\|4502654_4504223_+	hypothetical protein	NA	NA	NA	NA
AVA35871.1\|4504324_4506010_+\|integrase	integrase	NA	NA	NA	NA
AVA35872.1\|4507005_4508982_-	cellulose synthase	M1HVH2	Acanthocystis_turfacea_Chlorella_virus	3.1e-27	27.9
AVA35873.1\|4508996_4510352_-	hypothetical protein	NA	NA	NA	NA
AVA35874.1\|4510422_4511754_-	multidrug transporter	NA	NA	NA	NA
AVA35875.1\|4511875_4513085_+\|transposase	IS3 family transposase	A0A1B0Z042	Pseudomonas_phage	1.1e-99	62.7
AVA35876.1\|4513555_4513987_+	universal stress protein	NA	NA	NA	NA
AVA35877.1\|4514199_4515039_+	class II glutamine amidotransferase	NA	NA	NA	NA
AVA35878.1\|4515038_4515896_+	AraC family transcriptional regulator	NA	NA	NA	NA
AVA35879.1\|4515928_4517782_-	acyl-CoA synthetase	NA	NA	NA	NA
AVA35880.1\|4518217_4518763_-	DUF2726 domain-containing protein	NA	NA	NA	NA
AVA35881.1\|4519128_4519662_-	ATP-binding protein	NA	NA	NA	NA
AVA35882.1\|4519890_4521357_-	RND transporter	NA	NA	NA	NA
AVA35883.1\|4521356_4522553_-	efflux RND transporter periplasmic adaptor subunit	NA	NA	NA	NA
AVA35884.1\|4522549_4525747_-	RND transporter	S5VTK5	Leptospira_phage	4.8e-62	21.8
AVA35885.1\|4525940_4527127_-\|transposase	IS3-like element ISRme13 family transposase	A0A0P0I4A4	Acinetobacter_phage	3.2e-56	39.7
4527537:4527553	attR	NA	NA	NA	NA

DBSCAN-SWA_12

5763284 : 5773682

Roseobacter_phage(14.29%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AVA36918.1\|5763284_5764874_+	polynucleotide adenylyltransferase PcnB	A0A1B0V011	Roseobacter_phage	4.5e-21	31.2
AVA36919.1\|5764875_5765397_+	2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase	NA	NA	NA	NA
AVA36920.1\|5765420_5766062_+	deoxynucleoside kinase	A0A1J0F9Q3	Only_Syngen_Nebraska_virus	6.3e-06	27.0
AVA36921.1\|5766198_5767020_+	3-methyl-2-oxobutanoate hydroxymethyltransferase	A0A0P0BXC9	Ostreococcus_lucimarinus_virus	1.5e-36	33.3
AVA36922.1\|5767027_5767726_+	ABC transporter permease	NA	NA	NA	NA
AVA36923.1\|5767722_5768412_+	ABC transporter ATP-binding protein	G9BWD6	Planktothrix_phage	1.9e-16	32.5
AVA37927.1\|5768442_5770338_-	aminodeoxychorismate synthase, component I	S4VT78	Pandoravirus	1.1e-50	34.9
AVA36924.1\|5770421_5771561_-	molecular chaperone DnaJ	A0A0P0YMJ9	Yellowstone_lake_phycodnavirus	1.2e-23	35.5
AVA36925.1\|5771735_5773682_-	molecular chaperone DnaK	A0A1V0SH73	Hokovirus	1.4e-144	49.5

DBSCAN-SWA_13

6294611 : 6300665

uncultured_Caudovirales_phage(33.33%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AVA37364.1\|6294611_6295199_-	phosphoheptose isomerase	A0A067XQR2	Caulobacter_phage	2.0e-14	34.0
AVA37960.1\|6295229_6295634_-	YraN family protein	NA	NA	NA	NA
AVA37365.1\|6295686_6296586_+	16S rRNA (cytidine(1402)-2'-O)-methyltransferase	M1PLC5	Streptococcus_phage	6.3e-36	40.6
AVA37366.1\|6296670_6297324_-	septal ring lytic transglycosylase RlpA family protein	F5B3X9	Synechococcus_phage	1.9e-18	53.9
AVA37367.1\|6297722_6298373_+	MBL fold metallo-hydrolase	NA	NA	NA	NA
AVA37368.1\|6298376_6299024_+	exonuclease	A0A2L0UZL4	Agrobacterium_phage	3.5e-36	40.5
AVA37369.1\|6299231_6299462_+	hypothetical protein	NA	NA	NA	NA
AVA37370.1\|6299553_6299913_-	arsenate reductase (glutaredoxin)	A0A2H4J8T1	uncultured_Caudovirales_phage	1.4e-18	44.4
AVA37961.1\|6300104_6300665_+	GNAT family N-acetyltransferase	A0A2H4J136	uncultured_Caudovirales_phage	1.2e-21	39.3

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

Overview of predicted results

Overview of the results

Cas Category Instructions

Results visualization

1. CP026546

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection

2. CP026547

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Click the colored protein region to show detailed information

Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection

3. CP026544

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection