CRISPRimmunity

Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Self targeting spacer number	Target MGE spacer number	Prophage number	Anti-CRISPR protein number
CP020114	Nodularia spumigena UHCC 0039 chromosome, complete genome	13 crisprs	cas14j,Cas9_archaeal,c2c9_V-U4,cas14k,csa3,PD-DExK,RT,DinG,Cas14c_CAS-V-F,2OG_CAS,WYL,cas2,cas1,csm6,cas6,csm5gr7,csm4gr5,csm3gr7,csm2gr11,cas10,csx3	2	5	0	0
CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	2 crisprs	Cas14u_CAS-V,RT	0	13	0	0

1. CP020114

CRISPR-Cas detection and classification

Crispr_ID: CP020114_1

• CRISPR_ID: CP020114_1
• CRISPR_location: 389582-389677
• CRISPR_type: TypeII
• Repeat_type: NA
• Spacer_info: 1 spacers
• Cas_protein_info: Cas9_archaeal

Consensus repeat of CP020114_1

Consensus_repeat	Method
GATACCAATGATTTGGGATAATT	CRISPRCasFinder

spacers of CP020114_1

>1.1|389605|50|CP020114|CRISPRCasFinder
GTCTGCGTCCATCTGCATTTTTAAATGGCTTTTACCAATATATGCCGACG

CRISPR arrays and Neighbor proteins around CP020114_1

CRISPR arrays

The CRISPR arrays of CP020114_1

>merge|CP020114|1|389582-389677|CRISPRCasFinder
GATACCAATGATTTGGGATAATTGTCTGCGTCCATCTGCATTTTTAAATGGCTTTTACCAATATATGCCGACGGACACCAATGATTTGGGATAATT

>CP020114|1|1|389582-389677|CRISPRCasFinder
GATACCAATGATTTGGGATAATT	GTCTGCGTCCATCTGCATTTTTAAATGGCTTTTACCAATATATGCCGACG
GACACCAATGATTTGGGATAATT

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
CP020114.1|AVZ29602.1|356560_358846_-|chaperone-protein-DnaJ	unknown	unknown	gnl|CDD|372576
CP020114.1|AVZ29614.1|394770_396090_-|GDP-mannose-dependent-alpha-mannosyltransferase	unknown	unknown	gnl|CDD|340831
CP020114.1|AVZ29621.1|412414_412831_-|hypothetical-protein	unknown	unknown	gnl|CDD|366351
CP020114.1|AVZ29613.1|394490_394751_-|hypothetical-protein	unknown	unknown	gnl|CDD|273084
CP020114.1|AVZ29607.1|377351_379046_-|hypothetical-protein	unknown	unknown	unknown
CP020114.1|AVZ29604.1|373833_375111_-|hypothetical-protein	Cas9_archaeal	cas9_archaeal	gnl|CDD|379519
CP020114.1|AVZ29603.1|369221_370220_-|UDP-glucose-4-epimerase	unknown	unknown	gnl|CDD|224012
CP020114.1|AVZ29620.1|410932_412327_-|metalloprotease-TldD	unknown	unknown	gnl|CDD|223389
CP020114.1|AVZ29616.1|399550_399910_-|hypothetical-protein	unknown	unknown	unknown
CP020114.1|AVZ29606.1|376355_377090_-|hypothetical-protein	unknown	unknown	gnl|CDD|370448
CP020114.1|AVZ29608.1|379178_379376_-|hypothetical-protein	unknown	unknown	gnl|CDD|379519
CP020114.1|AVZ29615.1|396740_398789_-|serine/threonine-protein-kinase-F	unknown	unknown	gnl|CDD|238121
CP020114.1|AVZ29612.1|393995_394439_-|putative-S-adenosylmethionine-dependent-methyltransferase	unknown	unknown	gnl|CDD|369777
CP020114.1|AVZ29619.1|408132_408825_-|hypothetical-protein	unknown	unknown	gnl|CDD|224001
CP020114.1|AVZ29611.1|387650_388397_-|hypothetical-protein	unknown	unknown	gnl|CDD|113257
CP020114.1|AVZ29617.1|400250_400682_-|hypothetical-protein	unknown	unknown	unknown
CP020114.1|AVZ29605.1|375569_376241_-|5'-nucleotidase-SurE	unknown	unknown	gnl|CDD|234732
CP020114.1|AVZ29610.1|385149_385926_-|putative-iron-export-permease-protein-FetB	unknown	unknown	gnl|CDD|377092
CP020114.1|AVZ29609.1|383144_385010_-|hypothetical-protein	unknown	unknown	gnl|CDD|315987
CP020114.1|AVZ29618.1|407424_408033_-|cytochrome-b6	unknown	unknown	gnl|CDD|224209

HMMScan for Signature genes

Protein	Cas_name	Cas_description	E-value	Identity	Coverage
CP020114.1|AVZ29604.1|373833_375111_-|hypothetical-protein	Cas9_archaeal	cas9_archaeal	1.2e-13	45.2	2.3

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
CP020114.1|AVZ29602.1|356560_358846_-|chaperone-protein-DnaJ	gnl|CDD|372576	pfam13355, DUF4101, Protein of unknown function (DUF4101). This is a family of uncharacterized proteins, and is sometimes found in combination with pfam00226.	1.79194e-39
CP020114.1|AVZ29614.1|394770_396090_-|GDP-mannose-dependent-alpha-mannosyltransferase	gnl|CDD|340831	cd03801, GT4_PimA-like, phosphatidyl-myo-inositol mannosyltransferase. This family is most closely related to the GT4 family of glycosyltransferases and named after PimA in Propionibacterium freudenreichii, which is involved in the biosynthesis of phosphatidyl-myo-inositol mannosides (PIM) which are early precursors in the biosynthesis of lipomannans (LM) and lipoarabinomannans (LAM), and catalyzes the addition of a mannosyl residue from GDP-D-mannose (GDP-Man) to the position 2 of the carrier lipid phosphatidyl-myo-inositol (PI) to generate a phosphatidyl-myo-inositol bearing an alpha-1,2-linked mannose residue (PIM1). Glycosyltransferases catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. The acceptor molecule can be a lipid, a protein, a heterocyclic compound, or another carbohydrate residue. This group of glycosyltransferases is most closely related to the previously defined glycosyltransferase family 1 (GT1). The members of this family may transfer UDP, ADP, GDP, or CMP linked sugars. The diverse enzymatic activities among members of this family reflect a wide range of biological functions. The protein structure available for this family has the GTB topology, one of the two protein topologies observed for nucleotide-sugar-dependent glycosyltransferases. GTB proteins have distinct N- and C- terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. The members of this family are found mainly in certain bacteria and archaea.	3.81625e-54
CP020114.1|AVZ29621.1|412414_412831_-|hypothetical-protein	gnl|CDD|366351	pfam00875, DNA_photolyase, DNA photolyase. This domain binds a light harvesting cofactor.	7.43921e-06
CP020114.1|AVZ29613.1|394490_394751_-|hypothetical-protein	gnl|CDD|273084	TIGR00452, methyltransferase_putative, tRNA (mo5U34)-methyltransferase. This model describes CmoB, the enzyme tRNA (mo5U34)-methyltransferase involved in tRNA wobble base modification. [Unknown function, Enzymes of unknown specificity].	7.11705e-06
CP020114.1|AVZ29615.1|396740_398789_-|serine/threonine-protein-kinase-F	gnl|CDD|238121	cd00200, WD40, WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.	1.09628e-99
CP020114.1|AVZ29604.1|373833_375111_-|hypothetical-protein	gnl|CDD|379519	pfam14239, RRXRR, RRXRR protein. This domain is found in bacteria, eukaryotes and viruses, and is approximately 180 amino acids in length. It contains a conserved RRXRR motif. It is often found in association with pfam01844.	8.82116e-102
CP020114.1|AVZ29603.1|369221_370220_-|UDP-glucose-4-epimerase	gnl|CDD|224012	COG1087, GalE, UDP-glucose 4-epimerase [Cell envelope biogenesis, outer membrane].	0
CP020114.1|AVZ29620.1|410932_412327_-|metalloprotease-TldD	gnl|CDD|223389	COG0312, TldD, Predicted Zn-dependent proteases and their inactivated homologs [General function prediction only].	2.81413e-135
CP020114.1|AVZ29606.1|376355_377090_-|hypothetical-protein	gnl|CDD|370448	pfam09353, DUF1995, Domain of unknown function (DUF1995). This family of proteins are functionally uncharacterized.	7.56359e-89
CP020114.1|AVZ29608.1|379178_379376_-|hypothetical-protein	gnl|CDD|379519	pfam14239, RRXRR, RRXRR protein. This domain is found in bacteria, eukaryotes and viruses, and is approximately 180 amino acids in length. It contains a conserved RRXRR motif. It is often found in association with pfam01844.	1.51787e-08
CP020114.1|AVZ29612.1|393995_394439_-|putative-S-adenosylmethionine-dependent-methyltransferase	gnl|CDD|369777	pfam08241, Methyltransf_11, Methyltransferase domain. Members of this family are SAM dependent methyltransferases.	2.04549e-09
CP020114.1|AVZ29619.1|408132_408825_-|hypothetical-protein	gnl|CDD|224001	COG1075, LipA, Predicted acetyltransferases and hydrolases with the alpha/beta hydrolase fold [General function prediction only].	6.00116e-27
CP020114.1|AVZ29611.1|387650_388397_-|hypothetical-protein	gnl|CDD|113257	pfam04481, DUF561, Protein of unknown function (DUF561). Protein of unknown function found in a cyanobacterium, and the chloroplasts of algae.	9.86724e-98
CP020114.1|AVZ29618.1|407424_408033_-|cytochrome-b6	gnl|CDD|224209	COG1290, QcrB, Cytochrome b subunit of the bc complex [Energy production and conversion].	3.99252e-41
CP020114.1|AVZ29605.1|375569_376241_-|5'-nucleotidase-SurE	gnl|CDD|234732	PRK00346, surE, 5'(3')-nucleotidase/polyphosphatase; Provisional.	2.49118e-86
CP020114.1|AVZ29610.1|385149_385926_-|putative-iron-export-permease-protein-FetB	gnl|CDD|377092	pfam03649, UPF0014, Uncharacterized protein family (UPF0014).	3.38339e-97
CP020114.1|AVZ29609.1|383144_385010_-|hypothetical-protein	gnl|CDD|315987	pfam13424, TPR_12, Tetratricopeptide repeat.	2.8131e-07

Protein sequences

>CP020114.1|AVZ29611.1|387650_388397_-|hypothetical-protein
MTIHSTLQRAFAERSVLKVISGLNNFDRDRVAATIKAAELGGATFVDIAADAALVQMAKNLINLPVCVSAVEPEKFVAAVNAGADLIEIGNFDSFYAQGRKFEAEEVLALTHQTRALLPEITLSVTVPHILELDQQVQLAEELIKAGADIIQTEGGTSSQPAHSGSLGLIEKAAPTLAAAYEIARVVSVPVLCASGISSVTAALAIASGAAGVGVGSAINQLNSEVAMIAAVRGLVEALAIANSRAIV
>CP020114.1|AVZ29610.1|385149_385926_-|putative-iron-export-permease-protein-FetB
MEDLIKLDFVDLAIAVGLMAGAIGLSAWEKLGLELNLVTATGRTILQLLVLGYVLDFIFALDNVWGVLAILAVIITITAIVARNRISPKIPLVLPLVWGAIFISTAVTVLYANFLIIQPDRWYEPRYVIPLAGIVLGNAMNAAAIAGERLVSTMNSQSVEIETHLSLGATPEQAVSQYRKAAIRAAFLPTLNQMTLVGMVAIPGITTGQLLAGIRPIDAVSYEILIIFMVAASNLLTTILVTRGLYRQFFNSAAQLVR
>CP020114.1|AVZ29609.1|383144_385010_-|hypothetical-protein
MSESLPIRDSASADLSVRYLTFIDEIIESTLKGKISSVEMVYQMLQKNIISGTGEVFELVLSDRLSSIQSQIDSETDELKQAKANRKLRALKTIQTQWQRYSQQNQATEAIATAVREITTASTDERLTIFLRVIDPNQKHPPNTQQLQQLAKGLQQFAQVDADLPEISQGITRGLSTWQGLQDDLVSWMYEKNQQLGFGGVPGESGPWATWAKHVKSHVPQSLFRALAMGESAIEFAEKQSSISLSDWVEVALILQYLQRGLVNWFDQQAYNVQAGSKLSISTYLTFAVIWSQLASGFQSRAAYSNGCSQVMLQILRNFSQRPYFPLYGGIFASFAGNSLRDALDYLDEPLRRVEGTQEKARILTLLGYSQRALGQYERSIQFHQQALEIARNAGDRPCEIANLNHLSRTYVQEKNYTEAINNSQRALMLSRQAGERTAEANALVNLGYSEVMQAQKLEQVETEVYESAINYLEQGLKLSEQLGDIQSKALCFSSLGIAYLVIDQSSAAIPYLENGFKTAQVSGDLYLQGRNLAYLSEAYYNLQNSEKAIYTGSLGMYLLEQIASNEWRQPAGLLTILQGQMGAEAFQSLLQQHRPKIMAIIGVDGYDYIPQLLEIYKENI
>CP020114.1|AVZ29608.1|379178_379376_-|hypothetical-protein
MPTKPSRARRWMKKGKAVGKFNKLGIFYVQLVIMSKLLKAIKHIEVGLVATPKNKFLSLTKTGNG
>CP020114.1|AVZ29607.1|377351_379046_-|hypothetical-protein
MKVAVQQEDLEVLARSLHEQVFAAIPSGEIFQIKCAVKKDELMILTQHPVGVSVETEHIFVILKEALQSSLIYSEQRVQCFIRRFGEELPYARCSFIIEQQEQVRRPIPLSSSLTYTPSPIEDKPEEQFDPFQDTPDVLTTQSQRPVKSLLLGITLMGIGVFGTGFYLLTRPCVMSFCQELQTAEKLNLGSRQLMRSANSENQLVAIQQQLETATSDLISIPSWSSRYQEAEVLKTSLSMQSAKINQLLTAFQAADVAEKKMQATANSIEGLQDIQHSWRGAIAPLEAISSTSELYELVKPRLSKYRVSLQAVNQELVAQEQWLKKLNDAKAVAIVAKERETTAKSLNDWQKAQSTWQVAINALKIIPHTSPAYPEAQELLLVYEPRLAKTRISATIEKIASDNYQQAISTANQAKIYEQQNQWQVAVTYWTQALESAKQVSQQSFYYNQSQRLIEPYSTALKQAQEELQFLSRLEQVRNDLDQTCSREILVCTFTIHKTGIVISLTPNYEQVLQTTLSETELETTNHWYILQEALGVIGDSANLPVFIYNTQGQGLYTHIPQG
>CP020114.1|AVZ29606.1|376355_377090_-|hypothetical-protein
MPELPNSLEQAIAQSRIATQAALADGYTRLQVDFLFPELKLMPVAEQFLSLFTEYDSRLKIFFPDAGGAALANRDWAGTPFKIVDVGTGRAASIQSKIQPEDEIFLFIAPTSVEVPQVEKLCENIGDRPFVMLNPRLEDSGVVGIGYTARQTRQRFISTLESCYYLRPVDDTTAVFRCYPGLWEVWVEINGQYQKVAELPKRPTGDELDVIVMQGQPQTGTDAAPAKKPSVFKSLQRFFKALSS
>CP020114.1|AVZ29605.1|375569_376241_-|5'-nucleotidase-SurE
MLTNDDGIDAPGIQALFKALNDQKAIIAAPRDHQSGCGHQVTTTRGINLQRRSENEYAIAGTPADCVRIATSQICQNIKFVLSGINAGGNLGVDAYISGTVAAVREAATQDIPGIAISQYIKGKQSLDWDLAAKWTAEVLADLLQRDLEPGSFWNVNLPHLQPGDPHPQMVFCLPCTKPLPINYRIDGDDFYYVGEYSKRDRTPGSDVDVCFSGNIAVTQLKV
>CP020114.1|AVZ29604.1|373833_375111_-|hypothetical-protein
MSNFVLVLDTNKKPLTPIHPGDARFLLNQQKAAVFRRFPFTIILKEPKSEVPTQPIELKIDPGSKTTGFALVQNNKVIWGMELQHRGLAIKESLETRKGVRRGRRSRHTRYRQARFLNRTKPQGWLAPSLSHRVLTINTWVKRLCNFAPITDIVQELARFDLQQLENPEISGFEYQQGELQGYEVREYLLNKWNRKCAYCTAENVPLQVEHIKPKAKGGTNRISNLCLACEKCNIKKGTQDIEKFLAKKPELLKQILSQAKRPLKDASAVNSTRWALFNKLKETGLPITTGSGGLTKFNRTRLGLPKTHWIDAACVGKVETLKILTTKILTVKSTGHSCRRFCRINKFGFPCTEPKKIFTHVSTGDFVKATLHKDRKNITSGKYVSRVKTPTKNGCEIVINGFRVEFSTMKDITKVHCSDGYSYV
>CP020114.1|AVZ29603.1|369221_370220_-|UDP-glucose-4-epimerase
MSNGKPSILVTGGAGYIGSHTVLALKQAGYEVVILDNLVYGHQDLVEKVLQVELVVGDTGDRPLLDDLFKSRNITAVMHFSAYAYVGESVTDPAKYYRNNVVGTLTLLEAMLAASIHKFVFSSTCATYGVPEIVPIPENHPQNPINPYGATKLMVERILSDFDVAYGLKSVRFRYFNAAGAHPGGLLGEDHQPETHLIPLVLLTALGKRKSISIFGTDYPTPDGTCIRDYIHVNDLADAHVLGLQYLLENGDSEVFNLGNGNGFSVREVIAAAKEVTGLTIPVEECDRRPGDPPSLIGSGEKARKILNWQPQYPDIKNIVTHAWQWHQKRHL
>CP020114.1|AVZ29602.1|356560_358846_-|chaperone-protein-DnaJ
MRIPLDYYRILGLPLAASDEQLRQSYSDRIVQLPRREYSQPAICSRKQLIEEAYVVLSNPKERSSYDQLYLAHAYGPDGNGNATVAVGNHTEEGNNADPDIQSLSIDISQEELVGALLILQELGEYELVLKLGHNYLTTQNGVASARGENNLASEEFLESSDYPDIVLTVALACLELGREHWQQGHYENAAISLETGQELLGREGLFSSVQAEMQADLYKLRPYRILELLALPEEKVAQRRQGLAFLHDILSDRGGIDGTGNDQSGLNIDDFLRFIQQLRNYLTVAEQHKLFEAESKRPSAVATYLAVYALLARGFTQRQPALIRQAKQMLMRLGKRQDVHLEQSLCALLLGQTEEATRVLELSQEYEALALIREKSLDSPDLLPGLCLYAEQWLQNEVFLHFRDLATQQASLKDYFANQQVQAYLEALPNDAQTTHEWAVINRQSFSQPPLHSDHSTAAAPQFAQGRTPLPDLLATAAHKKPENGNFSPNSGNPLPDKITGDVYSHPDLADFSHPQRTTGATHPHLNGAAHPTSTGQVKKRRRRKPSQAVNRGYPQHQQAFAATLEGKTKLVWTVFGSLAGILVFWLLVSTTFGWVKNLLFPAPVLQGEQLFVELNEPPINIPDKSSKLQPPEGSLTDATAQEVIQTWLSTKAAALGPQYNLDSLEDILTGATLSQWRQLAQQERLQNRYRTYKHTVKVEYFDQKSDQAVVVATVREVTQFYDQGQIRNFTDETLRVRYSLIRQADVWRIQNMSVINQIG
>CP020114.1|AVZ29612.1|393995_394439_-|putative-S-adenosylmethionine-dependent-methyltransferase
MISADAEQFEWSERFDIVTSFETIEHLYDPEKFLERIHNLLIPDGVFLLCVPLGETRHMDRYHLHAFSQEEMFALLKKTGFSVDWHHCDECFLTRSELLRWGKLYPESSPSINELLFSRRGWRIIYDFVFRGGFDIPQVLIYARRIN
>CP020114.1|AVZ29613.1|394490_394751_-|hypothetical-protein
MKSEHNFPSNQLVLSNSLPLTWERIVPNQTANDSASQKFLDLHFGRYKTASSYVTDKKVLDIACGSGYGSRILRLAGAKSVVGVSR
>CP020114.1|AVZ29614.1|394770_396090_-|GDP-mannose-dependent-alpha-mannosyltransferase
MNNQPLRIAIFTAVYAPFLSGISIAVHQRVRWLLQQGHEVFLIHPQINDQYPKEVCSRPMLGLDELKSFPKFSAYAYPTKPLFYYKSAPEPLHHKHWSDTKLLENFQPDVVLVEEATQMRGFYSLFWGGYGRAIGTEYAHQTGTPTVSLFHTNAVGCIQYFLGNSLSRLIQPIIPLVSKRFSEAYDINFFSSKEQLAQYKKMQVKRGVYLPYQGVDCQKFHPKNICYDPIPDDHRPTMLFVGRITPEKNVTQLLEIYPIVAAKIPDIHLVIVGSGPQDEKILRLAQKFKSGVTIWGESHGTELLGWFARADIFVNPSFVENFCTTNNEALASGTPVVTTFAPSTSEQIFPSVNGFLAEPNNPKDFAEKVITILSNPDLKEEMSLRSRKSILEFDWSRCMEKLEEKLYQLVEASKPSEVLSVSSRKNTKERVVVNTLSSQ
>CP020114.1|AVZ29615.1|396740_398789_-|serine/threonine-protein-kinase-F
MICCLNPDCPNPLNLDKTKLCRTCSTPLVPLLRNRFRVIRVLSDEGGFGRTYLSEDTDKLNERCVVKQLAPKFEGTWSQKKAVELFAEEAKLLQELGEHPQIPTLLAYFEQDNCLYLVQQFVNGQNLLNELQRRKLYKPGEIHGILLHLLPVLQFIHDRGVIHRDIKPENIIRRYGDGRLSLIDFGSSKQLTARVQKKVGTSIGSHGYSPLEQIRDGKAYPASDLFGLGATCFHLLTGVSPFQLWMEYGYGWVTNWRQYLRSPLSAELDYVLDKLLRKDIQQRYQSADEVIRELIPKQPLALPPTGKSSGKIPVTQVSSLPVKYTLLKNVVLVSALILLFGIQDSWYQQYRRVHTVLLSRLSQTRSSSRKSVVDQSPNLNLKNISLSKVLQADQGLVLSVAISPDSQIFASSGERLIKVWHLGTGKEIATLKGHEQRVNVVAISPDGRTLVSGSDDQTIKIWDLFTGELIRTLRGHSNSIQAIAISPDGATLASGSDDNTIKLWNMSTGSLRSTLRGHTSWVRSLAISPDGITLVSGSFDKTIKMWDLNKAVFIDTLNSDTQTVTSIAFSPDGMTLASASRDVYGGKLRTIKLWNLATKHKSHTLAENAQTVTSIAFSPDNTTLVSGSRERTIKLWNLATGKEMGTLLGHKDTVTSVAISPDGKSLVSASEDNTIMIWRVSE
>CP020114.1|AVZ29616.1|399550_399910_-|hypothetical-protein
MKIVDFVLSILRPVRFVVVASACTLLFLSSVSPAFAISSYQSDPQEGPTQLLETQRRTDEVAKSQPLKLKEVQENSNKGLNEVQGDADIDKMKNPENSQSSTSVEDNVQNFLEKVTGKK
>CP020114.1|AVZ29617.1|400250_400682_-|hypothetical-protein
MLKLTYTESSFYLECLTESLEKWVAQRVVFALRVGQNLYVESSTASFLLPLDLPGIEMLKAEVKQNNSEIITLCSCDSEYMEVTLRGFWLSDNCEDAVGLFATTISDSSSEALSSASAIEVFLHQLWQDAQACASVMSEWYDS
>CP020114.1|AVZ29618.1|407424_408033_-|cytochrome-b6
MNSTQFDRIFRRLATILAVAILSLCLIYITTGILLSFYYEPTAGGAYASLKTINTEIPYGWLFRRAHDLAGNAVIVVALVQIVVMFLGRQFRKSWLTAWISGIFLTLSVIGLDWTAMLLDWTQEGYWRFSIELGTIEAIPFIGGQLRDILTGGGAISTVTVAHLNTIHSYILTLAAIILAFVHLSALIWQEQQMYKVRAFTS
>CP020114.1|AVZ29619.1|408132_408825_-|hypothetical-protein
MPLPTIIVPGYLESAIAYRQLEQSLLQLDIPTATVPLRRRDWLPTIGRPVTPIVQQLDRTVQQMLHKHNAAQVNLIGHSAGGWVSRIYLGDQPYLAPGQVTSCCWKAHSLVASLITLGTPHISQERWTRRNLDFVTQNYPGAFYKSVRYICVAGKTIFGQRRPGGWLAYNSYQQTCGQGNTWGDGITPIAAAHLEGAENLVIPGVQHSPRSPGIWYGSPEPLKSWVQYLL
>CP020114.1|AVZ29620.1|410932_412327_-|metalloprotease-TldD
MPSTLTDTQNILSDLISRYSPRVDYLVIRLEQSEGTDILLRGDQVETLSEGIAIGGHIRACYKGGWGFSSFNQLASIKERIEEAIAAARMVGDEKTILAPIDPVQAICQLPLTGTDPRKIPVGQKKELCDRYTDILKSVDPRITTTSVRYNDSAQKVIIATSEGTLIQQSWVDMEMRFAATARNGETVQTGKETTGSRTAYEDLTALDEQVKGAAQRAIAALTIPTVKGNTYTVVIDPILTGLFVHEAFGHLSEADMAYENPDLLEVMTIGRRFGTEELQIFDGAAPEGHRGSYFYDDEGTPSTTTQLIKDGILVGRLHSRETAGKLEEAPTGNARCLNYHFNPIVRMTNTWIQRGKTPVADLFSDIKSGVYARNWLGGMTNGEMFTFSAGEAWMIRNGKIAEPVRDVTLSGNAFQTLADIEAIGDDFYWDESGGCGKSGQNGLAVGCGGPSLRIRDVVVGGES
>CP020114.1|AVZ29621.1|412414_412831_-|hypothetical-protein
MSKPIVWVHGDSLSPYNPALQEYPKAPAIWVWDEELIEESQLSLKRITFIYECLLELPVIIRRGKVAQEILKFAQENHADLIVTSTSPSPRFNHICDQIEKTLPLETWETEPFFEYEGYIDLKRFSRYWKIAEKHLLT

Crispr_ID: CP020114_2

• CRISPR_ID: CP020114_2
• CRISPR_location: 725890-726003
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 1 spacers

Consensus repeat of CP020114_2

Consensus_repeat	Method
CTACGCCGAAGGCGCATCCCCGAAGGGGTTTAGGGCGATTTA	CRISPRCasFinder

spacers of CP020114_2

>2.1|725932|30|CP020114|CRISPRCasFinder
ACGGGATCTAAAATCAAAGTCCCCCTTTTC

CRISPR arrays and Neighbor proteins around CP020114_2

CRISPR arrays

The CRISPR arrays of CP020114_2

>merge|CP020114|2|725890-726003|CRISPRCasFinder
CTACGCCGAAGGCGCATCCCCGAAGGGGTTTAGGGCGATTTAACGGGATCTAAAATCAAAGTCCCCCTTTTCCTACGCCGAAGGCGCATCCCCGAAGGGGTTTAGGGGGATTTA

>CP020114|2|2|725890-726003|CRISPRCasFinder
CTACGCCGAAGGCGCATCCCCGAAGGGGTTTAGGGCGATTTA	ACGGGATCTAAAATCAAAGTCCCCCTTTTC
CTACGCCGAAGGCGCATCCCCGAAGGGGTTTAGGGGGATTTA

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
CP020114.1|AVZ29749.1|731142_732780_-|Na(+)/H(+)-antiporter-ApNhaP	unknown	unknown	gnl|CDD|223104
CP020114.1|AVZ29748.1|727044_730860_-|modification-methylase-PaeR7I	unknown	unknown	gnl|CDD|350769
CP020114.1|AVZ29747.1|726784_727006_-|hypothetical-protein	unknown	unknown	unknown
CP020114.1|AVZ29739.1|704772_705480_-|hypothetical-protein	unknown	unknown	unknown
CP020114.1|AVZ29741.1|708751_709102_-|putative-membrane-protein	unknown	unknown	gnl|CDD|224861
CP020114.1|AVZ29736.1|701350_702160_-|glucose-1-dehydrogenase	unknown	unknown	gnl|CDD|187616
CP020114.1|AVZ29752.1|747106_747475_-|hypothetical-protein	unknown	unknown	gnl|CDD|335963
CP020114.1|AVZ29738.1|704265_704697_-|hypothetical-protein	unknown	unknown	gnl|CDD|225105
CP020114.1|AVZ29755.1|759924_760932_-|quinone-oxidoreductase-1	unknown	unknown	gnl|CDD|176233
CP020114.1|AVZ29754.1|757657_759928_-|hypothetical-protein	unknown	unknown	gnl|CDD|379274
CP020114.1|AVZ29753.1|757089_757395_-|hypothetical-protein	unknown	unknown	unknown
CP020114.1|AVZ29750.1|732783_735780_-|hypothetical-protein	unknown	unknown	gnl|CDD|225743
CP020114.1|AVZ29740.1|707885_708680_-|superoxide-dismutase,-Fe-Mn-family	unknown	unknown	gnl|CDD|223678
CP020114.1|AVZ29743.1|711491_711683_-|hypothetical-protein	unknown	unknown	unknown
CP020114.1|AVZ29751.1|736096_736630_-|translation-initiation-factor-IF-3	unknown	unknown	gnl|CDD|234582
CP020114.1|AVZ29745.1|723509_724064_-|hypothetical-protein	unknown	unknown	gnl|CDD|225218
CP020114.1|AVZ29742.1|710146_711274_-|putative-zinc-metalloprotease-Rip3	unknown	unknown	gnl|CDD|100085
CP020114.1|AVZ29744.1|720789_722952_-|hypothetical-protein	unknown	unknown	gnl|CDD|225942
CP020114.1|AVZ29737.1|702298_703663_-|magnesium-transporter	unknown	unknown	gnl|CDD|129495
CP020114.1|AVZ29746.1|726071_726458_-|hypothetical-protein	unknown	unknown	gnl|CDD|238509

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
CP020114.1|AVZ29749.1|731142_732780_-|Na(+)/H(+)-antiporter-ApNhaP	gnl|CDD|223104	COG0025, NhaP, NhaP-type Na+/H+ and K+/H+ antiporters [Inorganic ion transport and metabolism].	2.41765e-86
CP020114.1|AVZ29742.1|710146_711274_-|putative-zinc-metalloprotease-Rip3	gnl|CDD|100085	cd06164, S2P-M50_SpoIVFB_CBS, SpoIVFB Site-2 protease (S2P), a zinc metalloprotease (MEROPS family M50B), regulates intramembrane proteolysis (RIP), and is involved in the pro-sigmaK pathway of bacterial spore formation. In this subgroup, SpoIVFB (sporulation protein, stage IV cell wall formation, F locus, promoter-distal B) contains two tandem repeats of the cystathionine beta-synthase (CBS pair) domain. SpoIVFB is one of 4 proteins involved in endospore formation; the others are SpoIVFA (sporulation protein, stage IV cell wall formation, F locus, promoter-proximal A), BofA (bypass-of-forespore A), and SpoIVB (sporulation protein, stage IV cell wall formation, B locus). SpoIVFB is negatively regulated by SpoIVFA and BofA and activated by SpoIVB. It is thought that SpoIVFB, SpoIVFA, and BofA are located in the mother-cell membrane that surrounds the forespore and that SpoIVB is secreted from the forespore into the space between the two where it activates SpoIVFB. It has been proposed that the CBS domain may play a regulatory role, although its exact function is unknown.	1.29016e-92
CP020114.1|AVZ29751.1|736096_736630_-|translation-initiation-factor-IF-3	gnl|CDD|234582	PRK00028, infC, translation initiation factor IF-3; Reviewed.	3.42067e-89
CP020114.1|AVZ29736.1|701350_702160_-|glucose-1-dehydrogenase	gnl|CDD|187616	cd05358, GlcDH_SDR_c, glucose 1 dehydrogenase (GlcDH), classical (c) SDRs. GlcDH, is a tetrameric member of the SDR family, it catalyzes the NAD(P)-dependent oxidation of beta-D-glucose to D-glucono-delta-lactone. GlcDH has a typical NAD-binding site glycine-rich pattern as well as the canonical active site tetrad (YXXXK motif plus upstream Ser and Asn). SDRs are a functionally diverse family of oxidoreductases that have a single domain with a structurally conserved Rossmann fold (alpha/beta folding pattern with a central beta-sheet), an NAD(P)(H)-binding region, and a structurally diverse C-terminal region. Classical SDRs are typically about 250 residues long, while extended SDRS are approximately 350 residues. Sequence identity between different SDR enzymes are typically in the 15-30% range, but the enzymes share the Rossmann fold NAD-binding motif and characteristic NAD-binding and catalytic sequence patterns. These enzymes have a 3-glycine N-terminal NAD(P)(H)-binding pattern (typically, TGxxxGxG in classical SDRs and TGxxGxxG in extended SDRs), while substrate binding is in the C-terminal region. A critical catalytic Tyr residue (Tyr-151, human 15-hydroxyprostaglandin dehydrogenase (15-PGDH) numbering), is often found in a conserved YXXXK pattern. In addition to the Tyr and Lys, there is often an upstream Ser (Ser-138, 15-PGDH numbering) and/or an Asn (Asn-107, 15-PGDH numbering) or additional Ser, contributing to the active site. Substrates for these enzymes include sugars, steroids, alcohols, and aromatic compounds. The standard reaction mechanism is a proton relay involving the conserved Tyr and Lys, as well as Asn (or Ser). Some SDR family members, including 17 beta-hydroxysteroid dehydrogenase contain an additional helix-turn-helix motif that is not generally found among SDRs.	1.10066e-126
CP020114.1|AVZ29752.1|747106_747475_-|hypothetical-protein	gnl|CDD|335963	pfam04972, BON, BON domain. This domain is found in a family of osmotic shock protection proteins. It is also found in some Secretins and a group of potential haemolysins. Its likely function is attachment to phospholipid membranes.	0.00245905
CP020114.1|AVZ29738.1|704265_704697_-|hypothetical-protein	gnl|CDD|225105	COG2194, COG2194, Predicted membrane-associated, metal-dependent hydrolase [General function prediction only].	0.00903061
CP020114.1|AVZ29741.1|708751_709102_-|putative-membrane-protein	gnl|CDD|224861	COG1950, COG1950, Predicted membrane protein [Function unknown].	3.17962e-19
CP020114.1|AVZ29755.1|759924_760932_-|quinone-oxidoreductase-1	gnl|CDD|176233	cd08272, MDR6, Medium chain dehydrogenases/reductase (MDR)/zinc-dependent alcohol dehydrogenase-like family. This group is a member of the medium chain dehydrogenases/reductase (MDR)/zinc-dependent alcohol dehydrogenase-like family, but lacks the zinc-binding sites of the zinc-dependent alcohol dehydrogenases. The medium chain dehydrogenases/reductase (MDR)/zinc-dependent alcohol dehydrogenase-like family, which contains the zinc-dependent alcohol dehydrogenase (ADH-Zn) and related proteins, is a diverse group of proteins related to the first identified member, class I mammalian ADH. MDRs display a broad range of activities and are distinguished from the smaller short chain dehydrogenases (~ 250 amino acids vs. the ~ 350 amino acids of the MDR). The MDR proteins have 2 domains: a C-terminal NAD(P)-binding Rossmann fold domain of a beta-alpha form and an N-terminal catalytic domain with distant homology to GroES. The MDR group contains a host of activities, including the founding alcohol dehydrogenase (ADH), quinone reductase, sorbitol dehydrogenase, formaldehyde dehydrogenase, butanediol DH, ketose reductase, cinnamyl reductase, and numerous others. The zinc-dependent alcohol dehydrogenases (ADHs) catalyze the NAD(P)(H)-dependent interconversion of alcohols to aldehydes or ketones. Active site zinc has a catalytic role, while structural zinc aids in stability. ADH-like proteins typically form dimers (typically higher plants, mammals) or tetramers (yeast, bacteria), and generally have 2 tightly bound zinc atoms per subunit. The active site zinc is coordinated by a histidine, two cysteines, and a water molecule. The second zinc seems to play a structural role, affects subunit interactions, and is typically coordinated by 4 cysteines.	6.17193e-165
CP020114.1|AVZ29754.1|757657_759928_-|hypothetical-protein	gnl|CDD|379274	pfam13576, Pentapeptide_3, Pentapeptide repeats (9 copies).	2.07048e-07
CP020114.1|AVZ29750.1|732783_735780_-|hypothetical-protein	gnl|CDD|225743	COG3202, COG3202, ATP/ADP translocase [Energy production and conversion].	1.51964e-06
CP020114.1|AVZ29740.1|707885_708680_-|superoxide-dismutase,-Fe-Mn-family	gnl|CDD|223678	COG0605, SodA, Superoxide dismutase [Inorganic ion transport and metabolism].	3.58117e-103
CP020114.1|AVZ29745.1|723509_724064_-|hypothetical-protein	gnl|CDD|225218	COG2340, COG2340, Uncharacterized protein with SCP/PR1 domains [Function unknown].	2.45631e-39
CP020114.1|AVZ29748.1|727044_730860_-|modification-methylase-PaeR7I	gnl|CDD|350769	cd18011, DEXDc_RapA, DEXH-box helicase domain of RapA. In bacteria, RapA is an RNA polymerase (RNAP)-associated SWI2/SNF2 (switch/sucrose non-fermentable) protein that mediates RNAP recycling during transcription. The ATPase activity of RapA is stimulated by its interaction with RNAP and inhibited by its N-terminal domain. The conformational changes of RapA and its interaction with RNAP are essential for RNAP recycling. RapA is part of the DEAD-like helicase superfamily, a diverse family of proteins involved in ATP-dependent RNA or DNA unwinding. This domain contains the ATP-binding region.	2.60033e-39
CP020114.1|AVZ29744.1|720789_722952_-|hypothetical-protein	gnl|CDD|225942	COG3408, GDB1, Glycogen debranching enzyme [Carbohydrate transport and metabolism].	5.24162e-90
CP020114.1|AVZ29737.1|702298_703663_-|magnesium-transporter	gnl|CDD|129495	TIGR00400, mgtE, Mg2+ transporter (mgtE). This family of prokaryotic proteins models a class of Mg++ transporter first described in Bacillus firmus. May form a homodimer. [Transport and binding proteins, Cations and iron carrying compounds].	0
CP020114.1|AVZ29746.1|726071_726458_-|hypothetical-protein	gnl|CDD|238509	cd01038, Endonuclease_DUF559, Domain of unknown function, appears to be related to a diverse group of endonucleases.	3.83117e-40

Protein sequences

>CP020114.1|AVZ29745.1|723509_724064_-|hypothetical-protein
MKNQLSHILWAVGAAMTLVLTGCEQVVEYLPPLPRVEIPSGQPPQPPQPTQSATTNQIEIAVRQRINEVRQNEDLHPLEHNEKLAQVARNYSRQMAENNFFSHTGNDDSTLQDRVRAGGMIYWVVGENLFKSTNVPQPVKVAVEGWMESSGHRENILRPVFTETGVGVWRVDNTYYITQLFLRR
>CP020114.1|AVZ29744.1|720789_722952_-|hypothetical-protein
MRVTVGPPILMINHGSTFMATDLAGEINPHSHLGLFSDDTRFLSHYACYVDGSPWIRLTSATTTYYAARVYLINPQFTSRQGKIAQGDLSLIISRTVEQGIHEDLDITNNSSKPVKFNLEIALGSDFADIFEVESQQCVRRGHIETRWREAENQLETSYKNDTFYRCLIYKPCNFTSQPHYANGRVTFEISLEPSQTWHTCCKYNLIDNKHVREAVDFCCEDMVNPTTINTEIERLHCQWRDSVTGIVCANEDVVRLYRQSVEDLGSLRLYDYDFEPDIWLPAAGVPKFVTLFGRDSLIISLQNMIIHPGFARGALQKLGQLQATELDDWRDAQPGKILHEVRQGELAHFGKVPHTPYYGTADATPLFLITLHETWKWLGDDSLLQNNRDKALRCLEWIDNYGDLDGDGFQEYQTQSSRGIENQGWKDSGNAVVYPDGTQVKAPKALCELQGYVFDAWMRMAEVFDVLGEGNFAQELRIKAAKLQVRFEEHFWSEDLDCYVFALDPQKKPVRSLTSNAGHCLWSGIASPERAARVVKRLMQPDMWSGWGIRTLSTENHAYNPYSYHLGSIWPHDNGIIAMGFKRYGFTAEAAQIAEGIFDAAKYFASYRLPELFAGIKREPAAFPVPYIEANVPQGWAAASVFHFIQAILGLEADAPHQLLYVDPHLPKWLPEIELQHVEIGNSRVDLQFWREGEITHWDAVVKSGEVEVKRQTWQPWRI
>CP020114.1|AVZ29743.1|711491_711683_-|hypothetical-protein
MFNPLLSMADKDETRMKCCYSLITIEDKHRLKVIGHLFPKSAKPATVERIIVFLVGADIFSTP
>CP020114.1|AVZ29742.1|710146_711274_-|putative-zinc-metalloprotease-Rip3
MQGFRLGSIFGFEIRVDLSWFLIFFLVLWTLSAGIFPTSYPGFGNATYFGMGIVATLLFFASLLAHELSHTFVARAKGIPVEGITLFIFGGVSRTRMDAETPGDEFQIAGVGPLVSLLLAGFFGLIWYVGINAGWSVVFTGIFAYLAVINLALAIFNMLPGFPLDGGRVFRSMIWKYTGNIKKATKIASTGGKWLAYLLIAFGALEMFAGAILGGLWLILIGWFLYNAAETSYEELLLRTSLKGVKAREIMTPHPETVSPEMNLQELVDTYFLSRRYHSFPVTQDSHPVGIITLNQVKDIPREEWKYRTVKDTMIPVENRVTARPEEQMSQVLQKMQDSGVRRVLVIQNDLLRGIITAHDLANWLQRQRDLDQVT
>CP020114.1|AVZ29741.1|708751_709102_-|putative-membrane-protein
MLNLLLTWLVTTISFLIISRLPIGIEIDSLGKAAISAAVFGVLNAILLPILSLVTLPAIILSFGLFFFVLNALIFALAAYLVPGFSLRWGFWSALIGSIALAIVNSILLSIVRTGV
>CP020114.1|AVZ29740.1|707885_708680_-|superoxide-dismutase,-Fe-Mn-family
MSKFIREKIAFVLTGMLLAMMLISCQPVPVAESQSPQGTPIAATFPPVASPDGELSASPAKLPLLPYDYAALGKAIDAETMKLHHDGHHASYVNNLNDALKQAPDLQKNSVEALLRDLNNVPENIRTKVRNNGGGHLNHTIFWQIMSPEGGGEPIEEIAAEINQTFGSFEEFKKQFNAAGGDRFGSGWVWLVRNPAGELQIVTTANQDNPIMDGAYPIMGNDVWEHAYYLRYRNRRAEYLKNWWNVVNWPEVNKRSLVNLQKGT
>CP020114.1|AVZ29739.1|704772_705480_-|hypothetical-protein
MSSRISIFILGILASISLVLIASPAVIIPPSDPVEPVTVYVLDLGLHSSLVLPDNDDKLIRYAYGDWRYFALNQQDLASGVAALLIPTQGTLARRKYENIAQLQQAINNHTTILNFAVARAKATGLQKQNFPGLNELPNQLIEQLAANLTQSAYTTLVKALEYPIGAEITARLTKNFPDALEIELQKKHNLQELEQLLVDMLEEIKINYVKNIAEEGIEKTVEEVENLQRQFRRY
>CP020114.1|AVZ29738.1|704265_704697_-|hypothetical-protein
MMNVGFFHRIPVMPLVLLWLAYALLGWYLAAHHIVWLAGAFVVAVTLAIVRKTIVWLEQLVAFGSRILVVVLFLSLSVALVETWSIFFSLFLIPIATTFLADLEMRFAGFNKTDSFWVLTVLALCGLVVGELIDILLLPSSRF
>CP020114.1|AVZ29737.1|702298_703663_-|magnesium-transporter
MQSRSRQELQDLVRQQLQVLLEQKNFQGAKALLIPVQVADIAESIEGLPEATQAIAFRLLSKEEAIEVFEYLDPSVQQTLLEDFKRQDVQEIVDYMSPDDRARLFDELPAKVVRRLLQQLSPAERDATALLLGYAPNTAGRIMTPEYLSLRENITINQALAQIRSLANLRETIYYIYVTDDARRLTGILSLRDLILAQPEQTIGSIMTRDVVFVNTGTDQEEVARTIQHYDFVAIPVVDAEQRLVGIVTVDDVLDILEQETTEDIYKLGGVESGGDNYFQTNLFTVARKRVVWLFILLITNSATAAVITNQEDILEKVVALTAFIPLLIGTGGNVGAQSSTVVIRGLNTEDVRPSEALNIIKREAIAGVLLGTMLGSVVIVWAYFLQGNFFVALAVGISLLSICVLASVSGSALPFLFRAMGLDPALMSAPFITTAVDVLGVLIYLNLARWILG
>CP020114.1|AVZ29736.1|701350_702160_-|glucose-1-dehydrogenase
MKGLQGKNALVTGATSGIGQAIAVKLAQLGCNIAINYRRTPESGSETEAMAVEKACKDIENCGMRSLLVKGDVSQESDIVTMVNSVVNTFGSLDILINNAGIQRECPSHELTTADFDQVLAVNLRGAFICARETIKHLLSQNRSGVIINISSVHEIIPRPTYLSYSISKGGMENLTKTLALEYANRGIRVNAVAPGATVTPINQAWIDDPEKKAAVESHIPMCRAGTAEEMAASVAFLASDDAGYITGQTLFVDGGLTLYADFRENWSA
>CP020114.1|AVZ29746.1|726071_726458_-|hypothetical-protein
MKISNFHLPYNPQLVERAKTLRKNMTKAEKKLWYDYLRNFQFRVHRQRPIDNFIVDFYCPNLSLVIEVDGESHSTDLAQNYDRERTQILEGYGLRIIRFTNQEVLNAFAGVCATIEELIPPTPLLKGG
>CP020114.1|AVZ29747.1|726784_727006_-|hypothetical-protein
MQTPYVSQIPIPKATDTQEACVTKIVDKILEIKRQNSKADTRELEREIDEIVYQLYGLTEEEIRIIEESVKRK
>CP020114.1|AVZ29748.1|727044_730860_-|modification-methylase-PaeR7I
MSDHQLSVFQSGIRDNRNRGTVGDFLKDKIELGAKLSIVSAYFTIYAFEALKQELCSIESLNFLFGEPRFIQSIGTKTDRKAFKIEDTGIQLSSRLKQSKLARECQEWIQKKVNIRSIKESNLLHGKMYHIDNNGVKTAILGSSNFTLKGLGLAKVNSNIELNLIVDSERDRQDLKFWFEELWNNHKLVQDVKDEIISYLNQLYQDNAPEFIYYKTLYHLFKQFLDQQAASDLLTKQGHLIDTKIWNLLLDFQKDGVKGAINKIMEYNGCIIADSVGLGKTFEALAIIKYFELLNYKVLVLCPKKLRSNWTIYHAANNSELNILVEDRFNYTVLSHTDLSRDTGYSGDINLETLNRGNYDLVVIDESHNFRNNTKGKRDEDGNVIKKSRYERLMEDIIKKGIPTRVLLLSATPVNTDLKDLRNQINFIVKDKDSAFSQTLGIDSIKQTLTTAQKEFTLWAKKKQHHSNELLENLNSSFFTLLDGLTIARSRQHIKKYYQETIAELGGFPERLKPVSLFSHIDLEDNFLDYDEINEQISDYQLSLFNPSNYVLEEYQHLYEGEVVQNNFTQSNRENYLIGMMKVNFLKRLESSVYSFRITLERTIDKIKKIESKIKNFGQHQIEIIKSEDCEIESFDDEELQAAFEVGRELKYQLEHLDIDRWLFDLARDRRQLHKLYIQAKDVDVCRDAKLADLKTIIEDKVTNPTLNKQSKENKKVIIFTAFADTAKYLYDALVGWVTTELNINMALVTGGNGGNKTTLARKQTGSFYTPREIVNYMVDESLIAYLQNGLLDPPQPPLRRGENTEKVPLRSGENTENPSLRSGENTKKVPLSKGDLGGSEIEDKLRHLLSYSNDFHEFNDDEVDCLINAIDNLKIIDIACGSGAFPMGILQKLVFILEKLDPNNIKWKQQQKEKAISPVLKDIQVAKQISYEQARDEAIEKLQERLAEIEYEFQNEMDYPRKLFLIENCIFGVDIQPIAVQISKLRFFISLIVDQKVNNNQPNRGILPLPNLETKFVAANSLISINKPRKAQLELDLRNEEIKDKEKQLAEVRNHHFKARTPQTKRKCREDDKHLRQEIGELLKQQGLPDEIAVKLSEWDLYNQNLSANFFDPEWMFGVDYFDICIGNPPYVRHEKIQELKPSFKQQYDCFTGVADLYVYFFEQAIKLLKEKGILAYITSNKYFRSGYGEKLREFLVNRTQLKQLIDFGDAPVFTAIAYPSIIITVKHASSVSQKQNVDNKIKVLNWQIGKPIDNFLEVLNTESFYFGFS
>CP020114.1|AVZ29749.1|731142_732780_-|Na(+)/H(+)-antiporter-ApNhaP
MGSREWDFAILRKFIKAELYQILLMNIESAIGEEIITTNLKQFLLVLSVSLGVATLPQIFSWFRHIPYTLLLVIVGLGLAFVDVRLVTLSPELILFIFLPPLLFEAAWNLRWAELKRNLVTICLYAVLGVVISITGIAFGLNQLAGISLTTALLIGASLSATDPVSVTALFRELGVGKSLTTMMEGESLFNDGMAVVAFGFMVALSLGNAELGVQPILFELVMVIGIGVAVGGLIGFGISYLTQRFDLPMVEQSLTLVSAYGTYLVIEELGGSGVIGVVTTGLILGNFGSRVGMNPRTRVIVSEFWDFLAFFVNSIVFLLIGDQIRFAVLGENLQIIGITIAAMILMRAVAIYLLSNLSAVITQHQIPLPEQTVLWWGGVRGSVSIALALSVPIGLPERETIIATVFGVVLFTLLVQGLTIKPLLQKLNLLGDAPLRDRYSELATRHVALERVLQHLQEDHRPGIDPEFYRYQETLIKGEMENLQTKIDKLQVEYPNLRTFITEQFREELLAIEADTYAEFVKAGRLNTELAPMLEDVWEHGDSH
>CP020114.1|AVZ29750.1|732783_735780_-|hypothetical-protein
MEFKNNSFAAKKGFLTRLLQWLNLRPEEGERTWLMFAFYTTVSVGLRWAEDSTVALFLDEYGAGPLPWMYIASAVMSMALVVVYSWLQKIFPLRWVIVAIAPCMVMPLIFIVSLRGGVYISYLTVALVFLLRLWVDGIYVINDLNTSIVANQLFNIREIKRTYPLVSSGMLVADVISGFSLPWMLQFIKLDQVILIACTVIILGSGILFYLSYHYREAFPDAPQRVIPQEQATRHRRLEAPLKRYTWQLFAFVGLLQIIGLLVDFQYLRELNASLSSVELASFLGLFGGVVGLCELITQWFISSRITERFGVFFTAALLPIAVGFILPGALALLNLVPVLQAQGFFWGLVGLKFVDELLRYTFVVSSGPVLYQPIPERIRSRTQTLSGGTAEAIATGLAGIIILVTLFVCNQFLPIEVQKWVLIGETVIIAGACLKVVLDLRSRYVDLLVLSAERGELSAANVGLRFFHQGVIKALSEKGSTADKRSCIELLAQIDPQGAAQVLAPLLLKLPPDLQRQSLEVMIMGNINPVYGLEVRSLLQQPPGTVDPEVFALALRYVWLADTNPNLTLLEEYLQPRHHSLIRATAAALILRQGTPLQKIAATKTMRRMLTHKQERERVNGVRALREAVYLQALRIHIPKLLQDESLRVRCAVLEIIAATHLEEYYSALVGALYYKSTRSTAMRALIRLENEALDILLQVATNNYKPEVVRMYAWRTIAQISSFEAIETLWTHLEISSGVNRHHILRSLLKINQQPEVTSIDTFHHSQVENLIEQELKFLGEIYAAYLDFQQPLPINENFQSDRTLVIASLLQRALEELEIDVKERLLLLLRLLYSPEKMQAATFNLKSESGANLAQGLEILDHTITLPTKALILNILDRRSPQEKLHYLIDAGLGNYQQMSVSDRLTRMLTLSHLLSDWCFACCFHFAQERRIRLNIQEILVALRHPTGFVREAAIAYLSVVSHRVLVELLPKLQQDTHPLVVAQVKELMQKNYGE
>CP020114.1|AVZ29751.1|736096_736630_-|translation-initiation-factor-IF-3
MPVIEKKRTRDLPQINERIRFPKIRVIDTDGAQLGIMPPQEALQLAEEKELDLVLLSDKADPPVCRIMDYGKYKFEQEKKAREARKKQHTADVKEVKMRYKIEEHDYNVRVKQAERFLKDGDKVKATVMFRGREIQHSDLAEHLLKRMAKDLEPLGELQQAPKKEGRNMMMLISPKK
>CP020114.1|AVZ29752.1|747106_747475_-|hypothetical-protein
MSWLKRLFGLEKPQNAQVNPTPQQVPPGSTTSAAPATTESIPPERLGLNGEYDQSGLAKRVALAFDQDSQLGDIDTLWVAQTSGTVVLKGNVPDQETLNKMVAVARSVNGATGVNTDEVTVG
>CP020114.1|AVZ29753.1|757089_757395_-|hypothetical-protein
MVINDLEYCELATDENRIIGGLDTGTNIESYVEPGYADITALATAVGDSSETFTETNTIVDTSSQFMVTKATGIALSRAWDSQGFSQSYQKQTVTLITNQY
>CP020114.1|AVZ29754.1|757657_759928_-|hypothetical-protein
MIEFFFKRKGARRSAQRGAEVRFWVSLRYFVIYVCVVWLFFSAPAGASSSQPERTSLSLELLQERLHTPVIREGNLTVDLREMVIDLRPENGEFRDNFYQVLRKELQRSGTKPLGLDLSNSLIQGNFVGSDLGLRTPLYAPVISSEQSVGVYAPVLTSSEQAQLEHLRFVCLQSLDIALPNSKDCRALLGSQPTTSSEINIFRGSLILVKTRFNGEVQFPNTFFFQPVNAKNAIFLQPTNWMETRFSRSVRFTGANFRQLSNFQTSVFFDKVNFQKAQFQETADFQDSTFEESTQFNEAIFKQSAKFSRAQWRKNADFSSVRFAETAQFTKANFHQYIFFTEAIFEQVVIFRDAIFNQAVNLSGASIFNQADFSDARFAKEAFLNVPGLSFNSNQGKILGNPGEIGKMFRVPTSQGNQNILRNLGQNFRQQQQIADANQLEYTKQKLRLRELSHGLVDGNINSATRKTLINLGFSAIQADAIANRRMSKLFRNSSELLSLADIDLETYNQLSDRLVVAEPLSPGGWLLLAARWLALSVLLLLSGYGTSFWLVFGVGGVAIAYFGWLFWLVDRYRRLHPVPIIPTYYETTWILAGFSFLTLFSLLAIFRNAEQPWLTLGCLLIIIIPVPVILLIQLYQQGRYHDLMDVSYFTEDGTFRQLRLLIGRLPVIPRNQTFRERYMHLLWNRRWNWLNYYDFSLNNLVRLGFNDIRLRDEHLPGIIATLAWYQWSLGILYITLVLWTLSRTIPGLNLLIYLK
>CP020114.1|AVZ29755.1|759924_760932_-|quinone-oxidoreductase-1
MKAVLMTAAGSPEVLQLQDVPKPTVPLENTQLLVRLVAAGVNPIDTKLRQRGTFYPEQMPAILGCDGAGIVEAVGAGVERFRVCDEVYFCYGGLGAHQGNYAEYTVVDERFVACKPASVSFAEAAAAPLVLITAWEALYERGRLAPGERVLIHAGAGGVGHVAIQLAKLKGASVCTTVSSQEKANFVKELGADEVIFYQETDFVQAALNWTGGEGVDLAFDTVGGETLHKTFPAVRVYGDIVTILEPKADTVWKAARLRNLRIGLELMLTPMLQGLEESLKHHGEILEQCAIWMDEGKLKVEVSHRFPLAEAAQAHQVLESGGMVGKVVLLMSDE

Crispr_ID: CP020114_4

• CRISPR_ID: CP020114_4
• CRISPR_location: 860769-860869
• CRISPR_type: TypeV-U4
• Repeat_type: NA
• Spacer_info: 1 spacers
• Cas_protein_info: c2c9_V-U4

Consensus repeat of CP020114_4

Consensus_repeat	Method
CCTTCTTAAGGGGGGCTAGGGGGGATCAA	CRISPRCasFinder

spacers of CP020114_4

>4.1|860798|43|CP020114|CRISPRCasFinder
AACGTTATGACGCAACTTGAAAATACTTGTGTGTACACCGTAG

CRISPR arrays and Neighbor proteins around CP020114_4

CRISPR arrays

The CRISPR arrays of CP020114_4

>merge|CP020114|4|860769-860869|CRISPRCasFinder
CCTTCTTAAGGGGGGCTAGGGGGGATCAAAACGTTATGACGCAACTTGAAAATACTTGTGTGTACACCGTAGCCTTCTTAAGGGGGGCTAGGGGGGATCAA

>CP020114|4|4|860769-860869|CRISPRCasFinder
CCTTCTTAAGGGGGGCTAGGGGGGATCAA	AACGTTATGACGCAACTTGAAAATACTTGTGTGTACACCGTAG
CCTTCTTAAGGGGGGCTAGGGGGGATCAA

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
CP020114.1|AVZ29812.1|874009_874303_-|hypothetical-protein	unknown	unknown	unknown
CP020114.1|AVZ29795.1|838646_840446_-|spore-coat-protein-A	unknown	unknown	gnl|CDD|259936
CP020114.1|AVZ29799.1|846503_848123_-|iron(III)-transport-system-permease-protein	unknown	unknown	gnl|CDD|224099
CP020114.1|AVZ29807.1|869437_870613_-|hypothetical-protein	unknown	unknown	gnl|CDD|214920
CP020114.1|AVZ29797.1|844184_845300_-|modification-methylase-AplI	unknown	unknown	gnl|CDD|223348
CP020114.1|AVZ29801.1|849302_850352_-|iron(III)-transport-system-substrate-binding-protein	unknown	unknown	gnl|CDD|270261
CP020114.1|AVZ29809.1|871896_872097_-|hypothetical-protein	unknown	unknown	unknown
CP020114.1|AVZ29798.1|845428_846469_-|iron(III)-transport-system-ATP-binding-protein	unknown	unknown	gnl|CDD|226361
CP020114.1|AVZ29802.1|855147_856362_-|hypothetical-protein	unknown	unknown	gnl|CDD|224563
CP020114.1|AVZ29806.1|868278_869232_-|DNA-polymerase-III-subunit-delta'	unknown	unknown	gnl|CDD|236011
CP020114.1|AVZ29811.1|873351_873954_-|hypothetical-protein	unknown	unknown	gnl|CDD|239738
CP020114.1|AVZ29803.1|858747_859056_-|hypothetical-protein	unknown	unknown	gnl|CDD|240845
CP020114.1|AVZ29794.1|837412_838159_-|hypothetical-protein	unknown	unknown	unknown
CP020114.1|AVZ29804.1|862591_864679_-|hypothetical-protein	unknown	unknown	gnl|CDD|224276
CP020114.1|AVZ29813.1|874335_874578_-|hypothetical-protein	unknown	unknown	unknown
CP020114.1|AVZ29796.1|843856_844195_-|type-2-restriction-enzyme-AplI	unknown	unknown	gnl|CDD|377679
CP020114.1|AVZ29810.1|872267_873368_-|putative-transposase	c2c9_V-U4	-	gnl|CDD|223747
CP020114.1|AVZ29805.1|865336_866752_-|hypothetical-protein	unknown	unknown	gnl|CDD|253386
CP020114.1|AVZ29800.1|848126_849152_-|Fe(3+)-binding-periplasmic-protein	unknown	unknown	gnl|CDD|270261
CP020114.1|AVZ29808.1|870941_871727_-|phosphoglycolate-phosphatase	unknown	unknown	gnl|CDD|223620

HMMScan for Signature genes

Protein	Cas_name	Cas_description	E-value	Identity	Coverage
CP020114.1|AVZ29810.1|872267_873368_-|putative-transposase	c2c9_V-U4	-	7.1e-100	329.7	3.3

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
CP020114.1|AVZ29802.1|855147_856362_-|hypothetical-protein	gnl|CDD|224563	COG1649, COG1649, Uncharacterized protein conserved in bacteria [Function unknown].	1.31354e-147
CP020114.1|AVZ29799.1|846503_848123_-|iron(III)-transport-system-permease-protein	gnl|CDD|224099	COG1178, ThiP, ABC-type Fe3+ transport system, permease component [Inorganic ion transport and metabolism].	3.0734e-119
CP020114.1|AVZ29807.1|869437_870613_-|hypothetical-protein	gnl|CDD|214920	smart00933, NurA, NurA nuclease. This family includes NurA a nuclease exhibiting both single-stranded endonuclease activity and 5'-3' exonuclease activity on single-stranded and double-stranded DNA from the hyperthermophilic archaeon Sulfolobus acidocaldarius.	8.72359e-51
CP020114.1|AVZ29797.1|844184_845300_-|modification-methylase-AplI	gnl|CDD|223348	COG0270, Dcm, Site-specific DNA methylase [DNA replication, recombination, and repair].	3.7132e-71
CP020114.1|AVZ29801.1|849302_850352_-|iron(III)-transport-system-substrate-binding-protein	gnl|CDD|270261	cd13543, PBP2_Fbp, Substrate binding domain of ferric iron transporter, a member of the type 2 periplasmic binding fold superfamily. The periplasmic iron binding protein plays an essential role in the iron uptake pathway of Gram-negative pathogenic bacteria from the Pasteurellaceae and Neisseriaceae families and is critical for survival of these pathogens within the host. This periplasmic protein (Fbp) has high affinities for ferric iron and serves as the primary receptor for transport. After binding iron with high affinity, Fbp interacts with a cognate membrane transport complex comprised of two integral membrane domains and two cytoplasmically located ATPase. This interaction triggers the ligand translocation across the cytoplasmic membrane energized by ATP hydrolysis. The ferric iron-binding proteins belong to the PBP2 superfamily of periplasmic binding proteins that differ in size and ligand specificity, but have similar tertiary structures consisting of two globular subdomains connected by a flexible hinge. They have been shown to bind their ligand in the cleft between these domains in a manner resembling a Venus flytrap.	4.15346e-140
CP020114.1|AVZ29798.1|845428_846469_-|iron(III)-transport-system-ATP-binding-protein	gnl|CDD|226361	COG3842, PotA, ABC-type spermidine/putrescine transport systems, ATPase components [Amino acid transport and metabolism].	4.26143e-141
CP020114.1|AVZ29806.1|868278_869232_-|DNA-polymerase-III-subunit-delta'	gnl|CDD|236011	PRK07399, PRK07399, DNA polymerase III subunit delta'; Validated.	7.25326e-166
CP020114.1|AVZ29811.1|873351_873954_-|hypothetical-protein	gnl|CDD|239738	cd03769, SR_IS607_transposase_like, Serine Recombinase (SR) family, IS607-like transposase subfamily, catalytic domain; members contain a DNA binding domain with homology to MerR/SoxR located N-terminal to the catalytic domain. Serine recombinases catalyze site-specific recombination of DNA molecules by a concerted, four-strand cleavage and rejoining mechanism which involves a transient phosphoserine linkage between DNA and the enzyme. They are functionally versatile and include resolvases, invertases, integrases, and transposases. This subfamily is composed of proteins that catalyze the transposition of insertion sequence (IS) elements such as IS607 from Helicobacter and IS1535 from Mycobacterium, and similar proteins from other bacteria and several archaeal species. IS elements are DNA segments that move to new sites in prokaryotic and eukaryotic genomes causing insertion mutations and gene rearrangements.	1.8204e-61
CP020114.1|AVZ29803.1|858747_859056_-|hypothetical-protein	gnl|CDD|240845	cd12399, RRM_HP0827_like, RNA recognition motif in Helicobacter pylori HP0827 protein and similar proteins. This subfamily corresponds to the RRM of H. pylori HP0827, a putative ssDNA-binding protein 12rnp2 precursor, containing one RNA recognition motif (RRM), also termed RBD (RNA binding domain) or RNP (ribonucleoprotein domain). The ssDNA binding may be important in activation of HP0827. .	3.72849e-28
CP020114.1|AVZ29795.1|838646_840446_-|spore-coat-protein-A	gnl|CDD|259936	cd13868, CuRO_2_CotA_like, The second Cupredoxin domain of bacterial laccases including CotA, a bacterial endospore coat component. CotA protein is an abundant component of the outer coat layer in bacterial endospore coat and it is required for spore resistance against hydrogen peroxide and UV light. Laccase is composed of three cupredoxin-like domains and includes one mononuclear and one trinuclear copper center. It is a member of the multicopper oxidase (MCO) family, which couples the oxidation of a substrate with a four-electron reduction of molecular oxygen to water. MCOs oxidize their substrate by accepting electrons at a mononuclear copper center and transferring them to the active site trinuclear copper center. The cupredoxin domain 2 of 3-domain MCOs has lost the ability to bind copper.	2.34461e-60
CP020114.1|AVZ29804.1|862591_864679_-|hypothetical-protein	gnl|CDD|224276	COG1357, COG1357, Pentapeptide repeats containing protein [Function unknown].	9.4217e-16
CP020114.1|AVZ29796.1|843856_844195_-|type-2-restriction-enzyme-AplI	gnl|CDD|377679	pfam06616, BsuBI_PstI_RE, BsuBI/PstI restriction endonuclease C-terminus. This family represents the C-terminus of bacterial enzymes similar to type II restriction endonucleases BsuBI and PstI (EC:3.1.21.4). The enzymes of the BsuBI restriction/modification (R/M) system recognize the target sequence 5'CTGCAG and are functionally identical with those of the PstI R/M system.	2.33412e-33
CP020114.1|AVZ29810.1|872267_873368_-|putative-transposase	gnl|CDD|223747	COG0675, COG0675, Transposase and inactivated derivatives [DNA replication, recombination, and repair].	8.4876e-46
CP020114.1|AVZ29805.1|865336_866752_-|hypothetical-protein	gnl|CDD|253386	pfam05787, DUF839, Bacterial protein of unknown function (DUF839). This family consists of several bacterial proteins of unknown function that contain a predicted beta-propeller repeats.	1.00921e-74
CP020114.1|AVZ29800.1|848126_849152_-|Fe(3+)-binding-periplasmic-protein	gnl|CDD|270261	cd13543, PBP2_Fbp, Substrate binding domain of ferric iron transporter, a member of the type 2 periplasmic binding fold superfamily. The periplasmic iron binding protein plays an essential role in the iron uptake pathway of Gram-negative pathogenic bacteria from the Pasteurellaceae and Neisseriaceae families and is critical for survival of these pathogens within the host. This periplasmic protein (Fbp) has high affinities for ferric iron and serves as the primary receptor for transport. After binding iron with high affinity, Fbp interacts with a cognate membrane transport complex comprised of two integral membrane domains and two cytoplasmically located ATPase. This interaction triggers the ligand translocation across the cytoplasmic membrane energized by ATP hydrolysis. The ferric iron-binding proteins belong to the PBP2 superfamily of periplasmic binding proteins that differ in size and ligand specificity, but have similar tertiary structures consisting of two globular subdomains connected by a flexible hinge. They have been shown to bind their ligand in the cleft between these domains in a manner resembling a Venus flytrap.	1.0222e-140
CP020114.1|AVZ29808.1|870941_871727_-|phosphoglycolate-phosphatase	gnl|CDD|223620	COG0546, Gph, Predicted phosphatases [General function prediction only].	1.43067e-07

Protein sequences

>CP020114.1|AVZ29803.1|858747_859056_-|hypothetical-protein
MSVYVGNLSYEVTQDTLSAVFAEYGSVKRVQLPTNRETGQLRGFGFVEMGTDDEETAAIEALDGAEWMGRDLKVNKAKPREDRGSFGGGNRSGGGGNFRSRY
>CP020114.1|AVZ29802.1|855147_856362_-|hypothetical-protein
MELMGGEFVHRQKQALFAVIVSLSMIVTVMLSLPLNAQTIPKSELRGVWLTNIDSNVLFERDRLKNALQRLDELNFNTVYPVVWNWGYTLYPSKVAAKVIGRSLDPEPGLQGRDILKEIVTEGHEKGLKVIPWFEFGFMAPADSLLAKNRPQWLTSRSDGSKIVKEGIHERVWLNPFHPEAQKFIQDLIVEIVSNYDIDGIQFDDHFGLPSELGYDAYTVALYKKEHRGQAPPQDPKNPAWVRWRANKITDFMKQVFTAIKANQKDCIVSVAPNPQRFSYEYYLADWQRWERMGLVEDLVLQIYRNDLNVFIKELEYPEVKAARSHIPVSIGILSGLKNRTVPIEQIKTQVEKVRDRKFAGVSFFFYETLWNLSQEQPVKRQTGLKKIFPTPTNYPNLLTGWKP
>CP020114.1|AVZ29801.1|849302_850352_-|iron(III)-transport-system-substrate-binding-protein
MKRRQFVYLVGIATASGGLAVACDANSNPTATDLGQTATELEKELVIYSGRNEKLIGELIKQFETQANTKVQVRYGDTAELASAILEEGTNSPADVFFAQDAGALGAIQKAGRTVELPTSLLNKVDSAYRSPEGQWVGVTGRVRTVDYNTNLVKAEELPSSIFGFTDPKWQGKIGWAPTNGSFQSFVTALRVAEGEERAKEWLEGIKANNAKVYPNNTSIVEALSRGEIAVGFVNHYYLERIKQDNPDVPVAHHFTEDIGSLVNVAGVAILNSAKNPNIAQRFAEFLLNEDAQNYFASETFEYPLASGVAPKGSLKSLSEIRKQDKQIDLSNLDDLDNTLKLLQEVAII
>CP020114.1|AVZ29800.1|848126_849152_-|Fe(3+)-binding-periplasmic-protein
MQTVKLKPLSLMKATAGALAFMLTSGIGLSVNAQTKTLTIYSGRGEKLIGPLLEQAKKDLGMNIQVRYGDTAELAIALVEEGKNSRADLYFAQDAGALGLLERRNLTLPISSNLLNQVDPRFRSPQGKWLGISGRARTLNYNTNLVKKEQLPKSVWDLVKPQWRGKVAWAPTNGSFQSFVTAMRVLEGEPRTLQWLKAMKANGVKDLRNNTAIVEALGRGEAQIGLVNNYYLANFKKTDPNFPVAAHYTNQDAASMFNIAGVAIMNSTKQKPEVEKFIGYLLRPSSQAYFAKETNEYPVVSGVAAPVNQVPLNQIKTPNVNLTNLNDLPGTLELLQQAGVL
>CP020114.1|AVZ29799.1|846503_848123_-|iron(III)-transport-system-permease-protein
MKSSVRPPLFLILAAAVVAVAITLPLVYLVIRAVGIGGDEFWALISRPRNIGVFLNSAAMAATVTLFSTLIAVPLAFLTVRTDLPGRKFWLVATTLPLAVPSYVGSFALIATLAPRGSFLQLLLQPLGVEELPSIYGFPGAVLAITLFTYPYLLLSVRSGLQGIDPSIEEAGRSLGYSSKETFFKVVLPQLKPSMIAGGLLVALYSLRDFGTPSLMRFDTFTRVIFIQYKASFNRNTAAVLALMLVTLVLLILWLEYRVRSRAAYYSRGSASLRPPKIVKLGIWKWPALGFCLLITSFGVVLPVGITLFWLIRGLNTGYSFPNLLPSILNSISGAGLAAIAATIFALPVAILSVRFPTKITAIIERCSYISFGVPGIVVALSLVFFGANYLPFLYQTLPMLVFAYLVLFLPQSVGAIRTSLLQVNPQLEESARSLGRNPWQALREVTLPLVRPGVISGAVLVFLTAIKELPATMLLAPIGFNTLATQIWQATENVDFADAAASSLAMLLVSMGSTLLVLSQENVKKEKVTPNAETQPKF
>CP020114.1|AVZ29798.1|845428_846469_-|iron(III)-transport-system-ATP-binding-protein
MQQAIVQLENVTKQFSQNVAPAVENVSLTLQQGDILGLLGPSGCGKTTLLRMIAGFENVQSGKIEIGGRIVSQGSVSVPAEQRDIGIVFQDYALFPHLNVAENVAFGLRHSTKQQIQKRISEVIELVNLQGLEKRYPYELSGGQQQRVALARALAPQPQLMLLDEPLSNLDIQVRLRLREEIRDILKAAGTSAIFVTHDQEEALAISDIVGVMKQGHLEQLGTPEEIYTHPASRFVAEFVTQANFLPARRQGNIWETEVGNFELPVNYTNDTGEIMIRQEGLKLEAARDSPVFIHSRRFLGREYLYCLKTASGKEIHARTRDDIPLPIGARVQVSVPGNTVKVFTQ
>CP020114.1|AVZ29797.1|844184_845300_-|modification-methylase-AplI
MKFRLPYLNFIETELQLPEKNHSEYLVIDLFGGCGGLALGFEATGFQTIGYEILADSRATYEHNLLGVCNQVNLTPFSNLVEGAAVIIGGPPCQPFSVSGHQLGLKDSRDGFPTFISAVERYRPQIALFENVRGMLFRNKKYFEEIVLALQEIGYIVEWEILNAAHYGVPQKRERLFCVAHKGSWQWPEKTHFYSPYTAGEALGKSAYLAPPNSKILTPSMDEYIKKYEIASKCINPRDINLDTPARTVTCRNLSSPTGDMLRIRLPDGRRRRLTVREGARLQSFPDWFQFQGSENHQFNQIGNAVPPLLAKALAHSVKTYLERNQQNLSQEFHQPTQYVQLSLELGINAVENTARNNRKLSKRGVVKDAG
>CP020114.1|AVZ29796.1|843856_844195_-|type-2-restriction-enzyme-AplI
MPDNLNSPDSILALQKIVEAHGKMPDVVVHFVEKNWLVLIEATTSHGPINPKRRNELKAIFGNSTAGLVFVTAFDRRSDMARYIQDLRKIMEKRTTKDTKDTKLRRFQRVLA
>CP020114.1|AVZ29795.1|838646_840446_-|spore-coat-protein-A
MNHSPYTFINMKNKVLFLGLFLFILVLSLNSVKAQSTNSYSLLNPNSQPQFINSLPSPQKIDATKGGNFTLEMKESEQWLGLYANPGEDGEYGTSDDERLNTTIWGYGLAGQNVTYPGPTFIAQESVPIRVEWENKLPREHLFPIDIEKLHSQPLREAFEQKLTPAVVHLHGGHTESASDGLPDEWYTQDYEITGPDWVQKIDLYDNNQEAATLWYHDHTNMVTRLNSYAGLAGYYLLRDDNENSLINNDVLPSGDYERELLIQDRKFTTDGQLFYPEQTNANPPGVPSRTGAYGDLILVNGMVWPKLEVEPRKYRLRLLNGSDSRFYKLEFYDSNKKMYLIGTEQGFVENPVALDHLVLSPSERADVIVDFTNDAGKEFILRNYGPGADPNTTGQIIKFTVNQPLSNIPIATVDTDESDGLTTQLRQENITPLTATVPPKQLALFQLREAGNNDLFLLGTLEDGSFKYSDPVTEKTLLDQTEVWELYNTTQYTHTIHIHLIAFQVLNRQNFRGNVVSEIDPKKGETKQYLRNVYFQSDPKDPEIYEQGRKDSIIVYPNEVVRVIAKYDRAGKYVWHCHVLIHEDHDMMRPMEIVTELE
>CP020114.1|AVZ29794.1|837412_838159_-|hypothetical-protein
MTLPEAYRSQIQHIQESAKFRLHSGSRQAAPFPGYSLITPPAQAEAKNSTFYTKLQSYQQELLQLPVDSDLIVPVPPVSFHLTLADLIWDSAYLHACEKDPEFDQQLRSCCAEILQQYQESMTNGTNPIQWQMLGLIVMPRSVGVCLVPQDERSYEQIIQFRRTIYQNPQLMALGIEQHYDFTAHVTLGYFGKVSPGLDRTNFSTMLSELNQQWLLNSPEFLIHRVELRKFDDMTHYYREPDWPSLNF
>CP020114.1|AVZ29804.1|862591_864679_-|hypothetical-protein
MPPNYAGQNLRGHSFKGQDLTGANFSKADIRGADFTNATLKNADFTDAKAGLQRRWTIGLLIASWLLSAVSGLFSIVLGALVAYIFHPDNTQDFIAGIVCLIVLLVFCFITLRKGLTAGAVAGAGAVAGAGAVAGAGAGAVALAVAGAGAVAGAGAGAGAVAGAVAVALAVAGAVAVAGAVAVAGAVAVAVAVALAVAVAGAGAGAVAGAGAGAFTLFSAYIGWRSLTGNGKEAWVRSFAVAFAATGGTSFRSADLTDADFSGAILRNTDFRKANLTRTRFYEVKKLDLARVGDSILAKRDVLNLLVTCNGRNKSYLGANLKGANLIGADLTNANLKDVDITETTFQGACLDGANLTLAQAVGTNFTNAKMTGACVEAWNIESTTQLNNVDCRFVYLLENPKPGTDDRERRPSSGDFQPGEFTKLFEEVLNTVDLIFRNGIDWKAFVNAFQNVQNQNEDTELGLQSIENKGDGVVVVKVGVPDGADKEKIHSDFTHYYQLGLQAVEEKYKALLNMQKEDIDFLRKQNAKIPEMIMSLANQSMNVKVDNKIENKPMTNSNDASRKINIGSVGGDFNASGQALNLGEISGTVTNTINELPSSPEPDELGVKELLAQLQAAIEAEKNLPEEDKAEALEQVKTLAEAGKSPQVEGAMQKAAKTALKILKGTVASLPSATKLVEECSKLLPLISQLLGLS
>CP020114.1|AVZ29805.1|865336_866752_-|hypothetical-protein
MEVSRRKFLTLAGTSAATVTMLSPLEAFYAKVARGEMPTSTGYGSLQEKLPLNTAELPADLSGIPILKLPRAFKYTAFSITGQPMDDGFTVARGHDGMAAFPGANNTTILVRNHELRTSSSDPVQGGIKYSTEAVGGTSTLVVGADRRLIKHFNSLAGTIRNCAGGPTPWRTWISCEETFSTTLNGSIRHGYNFEVIADPNSGLVNPVPLREMGRFNHEAVAVDPKTGYVYETEDRGDSCFYRFVPNVRPTKVGDLEKGGSLYALKVIGMNNFALNTINNPNFGGQPGLVKVGQTLPVEWVKIDNPDPDEESSSAGVGVRYEAQSKGAAIFFRGEGAWYGNGLVYFVASQGGPPVSSADNARGNGQVWAYNPAREEITLVVEASPSGAFLQAPDNITVAPFGDLFLCEDGSGTDYIVGVNKKGEVYQFAMNNITSGELAGACFSADGRTMFVNLQNPGITVAIFGPWNRRR
>CP020114.1|AVZ29806.1|868278_869232_-|DNA-polymerase-III-subunit-delta'
MTIDPFSPLLGQSQAIELLTQAVKQNRVAPAYLFVGIDGVGKTLAARCFVELLFSAQTRNIASLQSRLRQGNHPDLWWVQPTYQHEGKRLTAAEAAEKKLKRKAPPVIRLEQIREITEFLGRPPLEAPRNVVVLESAQTMAESAANALLKTLEEPGQATVILIAPSPESVLPTLVSRCQRIPFYRLDTQSLTQVLTQAGHEEILQHQTILSIAAGSPGSAIASYEQLQIIPETLLQDVTKVPSSYRQALELAKTIAQELDTEAQLWLVDYLQQSYWQQWHKAEIIHKLEKARKSLLCYAQPRLVWECTFIALLMHLS
>CP020114.1|AVZ29807.1|869437_870613_-|hypothetical-protein
MLDLTKLARQMQGLSEHLTLEAAASRQRLELAQQHLQKAYERQEDLINTQEQWRDRILFANATPIEPLETCIDIPVPPKVHTVIATDGSQIAPNHHEIAYCYLLNIGRVVLHYGQNRQPLLDSLPEVFYRPEDLYVSRQWGIKTEEWMGYRRTASETAVLAELACAVKGEAPALAMVDGSLIYWFLEQLPMDARDRILPPILEAWQQLNQAKIPVMGYLSASRNIDCTNFLRLLACPHPAPDCKSYCPDQLDKVPCKLFDTLRDTTLWGNQLQPGQRGPLWRSNNRILELYEQQIIYFCHVHVGSEIARIEVPAWVAEDTAMFNQALGLMLAQVQKGYGYPVAIAEAHNQAVVRGGDKTRFFALLERQMIKAGLKNVGTSYKETRKRGSIA
>CP020114.1|AVZ29808.1|870941_871727_-|phosphoglycolate-phosphatase
MTASSPTILALDFDGVICDGLIEYFEVAWRTYCQIWSPVDDTPPDDLPLRFYRLRPVIETGWEMPVLIKALVDQISEEKILQEWATITPQILLEHNLQSPTIGTALDNLRDEWITTDLDGWLSLHKFYPGVLEKIKLTIASETKLYIVTTKEGRFVQQLLQREGVNLPPAAIFGKEVKRPKYQTLRELIQKSEKKPVSLWFVEDRIKTLQLVQQQTDLENVKLFLADWGYNTQTERKAAQDDPGIQVINLPKFTKDFTGWL
>CP020114.1|AVZ29809.1|871896_872097_-|hypothetical-protein
MLAHGITHILTFNPSDFRLMPDIIIVRPQELVPQGGSQKSKVKSQKAYKMGFSGILNGLFIYAVLY
>CP020114.1|AVZ29810.1|872267_873368_-|putative-transposase
MLFGFKTELKINNHQRTQLLQHCGVARHAWNWGLALTKQILEHNKLNPNSKIKFPTAIDLHKWLVALVKSENPWYYECSKSAPQEALRALKTAWDRCFKKISGVPKFKRKGKHDSFTLEGSVKNLESNKIQVPKIGVLQTYERLPQKEIKSVTISRKANRWFISFRFDVEKQDNKNLKVVGVDLGVKNLATLSTGEIIEGAKSYKKYESKLSRLQWLNRNKVINSNNWRKAQLKIAKLHLKIAHIRKDTLHKLTTLLAKNHGKVVIEDLNVSGMLANRKLAKAISDMGFHEFRRQLTYKCELYSSELVVVDRWYPSSKTCSNCGTKKENLTLNERVFQCENCGFECDRDLNAAINLSKIDLSKAVS
>CP020114.1|AVZ29811.1|873351_873954_-|hypothetical-protein
MSKLSISEAAKLKGVSVSTLRRWETEGKLIPERTASGHRRYDLAQLLGIKPDLSYTIGYCRVSSHDQKEDLERQKQVVELFCAQNGWQFEIIEDLGSGLNYSKKGLKRLIRLIVDSKLERLVLTHKDRLLRFGSELIFSLCEHFGTEIVIINRTEDSTFEEDLAQDVLEIITVFSARLYGTRSHKNKKIIEELKEVAVRL
>CP020114.1|AVZ29812.1|874009_874303_-|hypothetical-protein
MRFCNPNDVHHDLATNAISCLLTQEDECYITAQVLVEFWVVATRPVEVNGLGWTVEQTRSTIDQLLDRFQLLEESAQIFPTWLSVVTTNKVMGLGTD
>CP020114.1|AVZ29813.1|874335_874578_-|hypothetical-protein
MLREKLKRELDKFNEEQLKQIADFIKFIEFKSKENTVPTPFWQRATPTERAKEFRKWVSQLPKNTPSLPDEAFSRDSIYE

Crispr_ID: CP020114_5

• CRISPR_ID: CP020114_5
• CRISPR_location: 952478-953238
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 10 spacers

Consensus repeat of CP020114_5

Consensus_repeat	Method
CTTTCCATAACCTCTTCCCCTAACGGGGATGGAAAC	PILER-CR
CTTTCCATAACCTCTTCCCCTAACGGGGATGGAAAC	CRISPRCasFinder
CTTTCCATAACCTCTTCCCCTAACGGGGATGGAAAC	CRT

spacers of CP020114_5

>5.1|952514|38|CP020114|PILER-CR,CRISPRCasFinder,CRT
GATAGGGAATAATTCCCAAAACCAGAAAGACAAAACAC
>5.2|952588|39|CP020114|PILER-CR,CRISPRCasFinder,CRT
TTTCCATCAACCCAAGTAAGCCAAACACCCATCAAAGTG
>5.3|952663|32|CP020114|PILER-CR,CRISPRCasFinder,CRT
CCGATAAGCGAAAAACACGCTTAAGGGTAATC
>5.4|952731|36|CP020114|PILER-CR,CRISPRCasFinder,CRT
TCTATGTAGCAGGTTGTCATTCCATTTGGATGGAGG
>5.5|952803|32|CP020114|PILER-CR,CRISPRCasFinder,CRT
TTGCTATGGCGTTTGATTTTGTTCCGAAAAGC
>5.6|952871|37|CP020114|PILER-CR,CRISPRCasFinder,CRT
CAGAGGGAAAGCAAGGTAATACTCACCCACCGCCAAT
>5.7|952944|36|CP020114|PILER-CR,CRISPRCasFinder,CRT
GCATCAACTTCATGGGTTTATCGCTCCATAACCTTG
>5.8|953016|39|CP020114|PILER-CR,CRISPRCasFinder,CRT
TTAAGAGAAGACATAATACATATCTCAATCTAAGGTTTA
>5.9|953091|36|CP020114|PILER-CR,CRISPRCasFinder,CRT
CCTTCTACCGCAGTATATGTTTTAGTTTTTTGTAGA
>5.10|953163|40|CP020114|PILER-CR,CRISPRCasFinder,CRT
CTACTTGAATAGGGTTATCTTTTTCTTCAAGTACAAAACC

CRISPR arrays and Neighbor proteins around CP020114_5

CRISPR arrays

The CRISPR arrays of CP020114_5

>merge|CP020114|5|952478-953238|PILER-CR,CRISPRCasFinder,CRT
CTTTCCATAACCTCTTCCCCTAACGGGGATGGAAACGATAGGGAATAATTCCCAAAACCAGAAAGACAAAACACCTTTCCATAACCTCTTCCCCTAACGGGGATGGAAACTTTCCATCAACCCAAGTAAGCCAAACACCCATCAAAGTGCTTTCCATAACCTCTTCCCCTAACGGGGATGGAAACCCGATAAGCGAAAAACACGCTTAAGGGTAATCCTTTCCATAACCTCTTCCCCTAACGGGGATGGAAACTCTATGTAGCAGGTTGTCATTCCATTTGGATGGAGGCTTTCCATAACCTCTTCCCCTAACGGGGATGGAAACTTGCTATGGCGTTTGATTTTGTTCCGAAAAGCCTTTCCATAACCTCTTCCCCTAACGGGGATGGAAACCAGAGGGAAAGCAAGGTAATACTCACCCACCGCCAATCTTTCCATAACCTCTTCCCCTAACGGGGATGGAAACGCATCAACTTCATGGGTTTATCGCTCCATAACCTTGCTTTCCATAACCTCTTCCCCTAACGGGGATGGAAACTTAAGAGAAGACATAATACATATCTCAATCTAAGGTTTACTTTCCATAACCTCTTCCCCTAACGGGGATGGAAACCCTTCTACCGCAGTATATGTTTTAGTTTTTTGTAGACTTTCCATAACCTCTTCCCCTAACGGGGATGGAAACCTACTTGAATAGGGTTATCTTTTTCTTCAAGTACAAAACCCTTTCCATAACCTCTTCCCCTAACGGGGATGGAAAC

>CP020114|5|1|952478-953238|PILER-CR
CTTTCCATAACCTCTTCCCCTAACGGGGATGGAAAC	GATAGGGAATAATTCCCAAAACCAGAAAGACAAAACAC
CTTTCCATAACCTCTTCCCCTAACGGGGATGGAAAC	TTTCCATCAACCCAAGTAAGCCAAACACCCATCAAAGTG
CTTTCCATAACCTCTTCCCCTAACGGGGATGGAAAC	CCGATAAGCGAAAAACACGCTTAAGGGTAATC
CTTTCCATAACCTCTTCCCCTAACGGGGATGGAAAC	TCTATGTAGCAGGTTGTCATTCCATTTGGATGGAGG
CTTTCCATAACCTCTTCCCCTAACGGGGATGGAAAC	TTGCTATGGCGTTTGATTTTGTTCCGAAAAGC
CTTTCCATAACCTCTTCCCCTAACGGGGATGGAAAC	CAGAGGGAAAGCAAGGTAATACTCACCCACCGCCAAT
CTTTCCATAACCTCTTCCCCTAACGGGGATGGAAAC	GCATCAACTTCATGGGTTTATCGCTCCATAACCTTG
CTTTCCATAACCTCTTCCCCTAACGGGGATGGAAAC	TTAAGAGAAGACATAATACATATCTCAATCTAAGGTTTA
CTTTCCATAACCTCTTCCCCTAACGGGGATGGAAAC	CCTTCTACCGCAGTATATGTTTTAGTTTTTTGTAGA
CTTTCCATAACCTCTTCCCCTAACGGGGATGGAAAC	CTACTTGAATAGGGTTATCTTTTTCTTCAAGTACAAAACC
CTTTCCATAACCTCTTCCCCTAACGGGGATGGAAAC

>CP020114|5|5|952478-953238|CRISPRCasFinder
CTTTCCATAACCTCTTCCCCTAACGGGGATGGAAAC	GATAGGGAATAATTCCCAAAACCAGAAAGACAAAACAC
CTTTCCATAACCTCTTCCCCTAACGGGGATGGAAAC	TTTCCATCAACCCAAGTAAGCCAAACACCCATCAAAGTG
CTTTCCATAACCTCTTCCCCTAACGGGGATGGAAAC	CCGATAAGCGAAAAACACGCTTAAGGGTAATC
CTTTCCATAACCTCTTCCCCTAACGGGGATGGAAAC	TCTATGTAGCAGGTTGTCATTCCATTTGGATGGAGG
CTTTCCATAACCTCTTCCCCTAACGGGGATGGAAAC	TTGCTATGGCGTTTGATTTTGTTCCGAAAAGC
CTTTCCATAACCTCTTCCCCTAACGGGGATGGAAAC	CAGAGGGAAAGCAAGGTAATACTCACCCACCGCCAAT
CTTTCCATAACCTCTTCCCCTAACGGGGATGGAAAC	GCATCAACTTCATGGGTTTATCGCTCCATAACCTTG
CTTTCCATAACCTCTTCCCCTAACGGGGATGGAAAC	TTAAGAGAAGACATAATACATATCTCAATCTAAGGTTTA
CTTTCCATAACCTCTTCCCCTAACGGGGATGGAAAC	CCTTCTACCGCAGTATATGTTTTAGTTTTTTGTAGA
CTTTCCATAACCTCTTCCCCTAACGGGGATGGAAAC	CTACTTGAATAGGGTTATCTTTTTCTTCAAGTACAAAACC
CTTTCCATAACCTCTTCCCCTAACGGGGATGGAAAC

>CP020114|5|1|952478-953238|CRT
CTTTCCATAACCTCTTCCCCTAACGGGGATGGAAAC	GATAGGGAATAATTCCCAAAACCAGAAAGACAAAACAC
CTTTCCATAACCTCTTCCCCTAACGGGGATGGAAAC	TTTCCATCAACCCAAGTAAGCCAAACACCCATCAAAGTG
CTTTCCATAACCTCTTCCCCTAACGGGGATGGAAAC	CCGATAAGCGAAAAACACGCTTAAGGGTAATC
CTTTCCATAACCTCTTCCCCTAACGGGGATGGAAAC	TCTATGTAGCAGGTTGTCATTCCATTTGGATGGAGG
CTTTCCATAACCTCTTCCCCTAACGGGGATGGAAAC	TTGCTATGGCGTTTGATTTTGTTCCGAAAAGC
CTTTCCATAACCTCTTCCCCTAACGGGGATGGAAAC	CAGAGGGAAAGCAAGGTAATACTCACCCACCGCCAAT
CTTTCCATAACCTCTTCCCCTAACGGGGATGGAAAC	GCATCAACTTCATGGGTTTATCGCTCCATAACCTTG
CTTTCCATAACCTCTTCCCCTAACGGGGATGGAAAC	TTAAGAGAAGACATAATACATATCTCAATCTAAGGTTTA
CTTTCCATAACCTCTTCCCCTAACGGGGATGGAAAC	CCTTCTACCGCAGTATATGTTTTAGTTTTTTGTAGA
CTTTCCATAACCTCTTCCCCTAACGGGGATGGAAAC	CTACTTGAATAGGGTTATCTTTTTCTTCAAGTACAAAACC
CTTTCCATAACCTCTTCCCCTAACGGGGATGGAAAC

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
CP020114.1|AVZ29860.1|974301_975381_-|hypothetical-protein	unknown	unknown	gnl|CDD|371736
CP020114.1|AVZ29859.1|973654_974245_-|hypothetical-protein	unknown	unknown	gnl|CDD|376806
CP020114.1|AVZ29842.1|929394_930030_-|HTH-type-transcriptional-repressor-ComR	unknown	unknown	gnl|CDD|224228
CP020114.1|AVZ29854.1|954886_955960_-|toluene-4-sulfonate-monooxygenase-system-iron-sulfur-subunit-TsaM1	unknown	unknown	gnl|CDD|226985
CP020114.1|AVZ29845.1|943076_943949_-|tRNA-pseudouridine-synthase-B	unknown	unknown	gnl|CDD|179469
CP020114.1|AVZ29848.1|947300_948482_-|hypothetical-protein	unknown	unknown	gnl|CDD|226925
CP020114.1|AVZ29843.1|936054_936801_-|putative-L,D-transpeptidase-YnhG	unknown	unknown	gnl|CDD|341130
CP020114.1|AVZ29856.1|965503_965953_-|hypothetical-protein	unknown	unknown	unknown
CP020114.1|AVZ29849.1|948566_950603_-|DNA-ligase	unknown	unknown	gnl|CDD|236137
CP020114.1|AVZ29851.1|950887_952012_-|serine/threonine-protein-kinase-B	unknown	unknown	gnl|CDD|270916
CP020114.1|AVZ29858.1|970914_971796_-|lactose-transport-system-permease-protein-LacF	unknown	unknown	gnl|CDD|224096
CP020114.1|AVZ29847.1|946235_947153_-|putative-inner-membrane-transporter-YhbE	unknown	unknown	gnl|CDD|307170
CP020114.1|AVZ29857.1|965994_968037_-|hypothetical-protein	unknown	unknown	gnl|CDD|366133
CP020114.1|AVZ29855.1|964299_964833_-|hypothetical-protein	unknown	unknown	unknown
CP020114.1|AVZ29844.1|942137_942944_-|hypothetical-protein	unknown	unknown	gnl|CDD|222907
CP020114.1|AVZ29852.1|954148_954343_-|hypothetical-protein	unknown	unknown	unknown
CP020114.1|AVZ29853.1|954416_954731_-|hypothetical-protein	unknown	unknown	unknown
CP020114.1|AVZ29861.1|976282_976465_-|hypothetical-protein	unknown	unknown	unknown
CP020114.1|AVZ29846.1|944067_945834_-|hypothetical-protein	unknown	unknown	gnl|CDD|226651
CP020114.1|AVZ29850.1|950622_950805_-|hypothetical-protein	unknown	unknown	unknown

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
CP020114.1|AVZ29859.1|973654_974245_-|hypothetical-protein	gnl|CDD|376806	pfam02517, Abi, CAAX protease self-immunity. Members of this family are probably proteases (after a isoprenyl group is attached to the Cys residue in the C-terminal CAAX motif of a protein to attach it to the membrane, the AAX tripeptide being removed by one of the CAAX prenyl proteases). The family contains the CAAX prenyl protease. The proteins contain a highly conserved Glu-Glu motif at the amino end of the alignment. The alignment also contains two histidine residues that may be involved in zinc binding. While they are involved in membrane anchoring of proteins in eukaryotes, little is known about their function in prokaryotes. In some known bacteriocin loci, Abi genes have been found downstream of bacteriocin structural genes where they are probably involved in self-immunity. Investigation of the bacteriocin-like loci in the Gram positive bacteria locus from Lactobacillus sakei 23K confirmed that the bacteriocin-like genes (sak23Kalphabeta) exhibited antimicrobial activity when expressed in a heterologous host and that the associated Abi gene (sak23Ki) conferred immunity against the cognate bacteriocin. Interestingly, the immunity genes from three similar systems conferred a high degree of cross-immunity against each other's bacteriocins, suggesting the recognition of a common receptor. Site-directed mutagenesis demonstrated that the conserved motifs constituting the putative proteolytic active site of the Abi proteins are essential for the immunity function of Sak23Ki - thus a new concept in self-immunity.	5.26214e-14
CP020114.1|AVZ29842.1|929394_930030_-|HTH-type-transcriptional-repressor-ComR	gnl|CDD|224228	COG1309, AcrR, Transcriptional regulator [Transcription].	7.592e-16
CP020114.1|AVZ29854.1|954886_955960_-|toluene-4-sulfonate-monooxygenase-system-iron-sulfur-subunit-TsaM1	gnl|CDD|226985	COG4638, HcaE, Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit [Inorganic ion transport and metabolism / General function prediction only].	1.5612e-66
CP020114.1|AVZ29845.1|943076_943949_-|tRNA-pseudouridine-synthase-B	gnl|CDD|179469	PRK02755, truB, tRNA pseudouridine synthase B; Provisional.	2.67763e-176
CP020114.1|AVZ29848.1|947300_948482_-|hypothetical-protein	gnl|CDD|226925	COG4552, Eis, Predicted acetyltransferase involved in intracellular survival and related acetyltransferases [General function prediction only].	4.0003e-114
CP020114.1|AVZ29843.1|936054_936801_-|putative-L,D-transpeptidase-YnhG	gnl|CDD|341130	cd16913, YkuD_like, L,D-transpeptidases/carboxypeptidases similar to Bacillus YkuD. Members of the YkuD-like family of proteins are found in a range of bacteria. The best studied member Bacillus YkuD has been shown to act as an L,D-transpeptidase that gives rise to an alternative pathway for peptidoglycan cross-linking. Another member Helicobacter pylori Csd6 functions as an L,D-carboxypeptidase and regulates helical cell shape and motility. The conserved region contains a conserved histidine and cysteine, with the cysteine thought to be an active site residue.	9.0392e-45
CP020114.1|AVZ29851.1|950887_952012_-|serine/threonine-protein-kinase-B	gnl|CDD|270916	cd14014, STKc_PknB_like, Catalytic domain of bacterial Serine/Threonine kinases, PknB and similar proteins. STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. This subfamily includes many bacterial eukaryotic-type STKs including Staphylococcus aureus PknB (also called PrkC or Stk1), Bacillus subtilis PrkC, and Mycobacterium tuberculosis Pkn proteins (PknB, PknD, PknE, PknF, PknL, and PknH), among others. S. aureus PknB is the only eukaryotic-type STK present in this species, although many microorganisms encode for several such proteins. It is important for the survival and pathogenesis of S. aureus as it is involved in the regulation of purine and pyrimidine biosynthesis, cell wall metabolism, autolysis, virulence, and antibiotic resistance. M. tuberculosis PknB is essential for growth and it acts on diverse substrates including proteins involved in peptidoglycan synthesis, cell division, transcription, stress responses, and metabolic regulation. B. subtilis PrkC is located at the inner membrane of endospores and functions to trigger spore germination. Bacterial STKs in this subfamily show varied domain architectures. The well-characterized members such as S. aureus and M. tuberculosis PknB, and B. subtilis PrkC, contain an N-terminal cytosolic kinase domain, a transmembrane (TM) segment, and mutliple C-terminal extracellular PASTA domains. The PknB subfamily is part of a larger superfamily that includes the catalytic domains of other protein STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase.	1.07637e-74
CP020114.1|AVZ29858.1|970914_971796_-|lactose-transport-system-permease-protein-LacF	gnl|CDD|224096	COG1175, UgpA, ABC-type sugar transport systems, permease components [Carbohydrate transport and metabolism].	2.68024e-89
CP020114.1|AVZ29847.1|946235_947153_-|putative-inner-membrane-transporter-YhbE	gnl|CDD|307170	pfam00892, EamA, EamA-like transporter family. This family includes many hypothetical membrane proteins of unknown function. Many of the proteins contain two copies of the aligned region. The family used to be known as DUF6. Members of this family usually carry 5+5 transmembrane domains, and this domain attempts to model five of these.	1.45434e-06
CP020114.1|AVZ29857.1|965994_968037_-|hypothetical-protein	gnl|CDD|366133	pfam00498, FHA, FHA domain. The FHA (Forkhead-associated) domain is a phosphopeptide binding motif.	1.05663e-11
CP020114.1|AVZ29860.1|974301_975381_-|hypothetical-protein	gnl|CDD|371736	pfam11805, DUF3326, Protein of unknown function (DUF3326). This protein is functionally uncharacterized. It is about 300-500 amino acids in length. This family is found in plants and bacteria.	0
CP020114.1|AVZ29844.1|942137_942944_-|hypothetical-protein	gnl|CDD|222907	PHA02611, 51, baseplate hub assembly protein; Provisional.	0.00247453
CP020114.1|AVZ29849.1|948566_950603_-|DNA-ligase	gnl|CDD|236137	PRK07956, ligA, NAD-dependent DNA ligase LigA; Validated.	0
CP020114.1|AVZ29846.1|944067_945834_-|hypothetical-protein	gnl|CDD|226651	COG4188, COG4188, Predicted dienelactone hydrolase [General function prediction only].	2.23994e-74

Protein sequences

>CP020114.1|AVZ29851.1|950887_952012_-|serine/threonine-protein-kinase-B
MSYCLNSHCLQPENAADVKFCLSCGSKLLLKDRYRAIKPIGQGGFGKTFLAVDEDKPSKPPCVIKQFYPQAQGTNSVQKAVELFTLEAVQLDDLGQHPQIPSLLAYFTQDDRQYLVQEFIDGQNLAEELAQKGAFSEAQIWQLLKDLLPVLQFCHSRGVIHRDIKPENILRDRNAKLVLVDFGASKSATGTALNQTGTSIGTPEYVAPEQMRGRALFASDIYSLGATCVHLLTARSPFDSYDINHDTWIWQKFLQTPLSSGLNRILEKMLVSIPMRRYQTADEVLKDLNQSPVVATPATTAKPQSTPTSVSKSSSKPDLELEEMKTQFLGIGKPQPNQPQPPKPISPPANNSVIDKELEELKAKFMGNNNSSSQ
>CP020114.1|AVZ29850.1|950622_950805_-|hypothetical-protein
MNTDDFFVGFIRSVLFVTDVRVKLKWVYLASIAIVNFNSADRSLTRMKPSNLGKLSLSIC
>CP020114.1|AVZ29849.1|948566_950603_-|DNA-ligase
MIQSQSEVKRVEELRQLLQQASYAYYVLDAPMMEDAVYDQLYRELQELEIKYPEFITPDSPTQRVGEKPATHFTSVRHNIPLYSLENAFNIDELQIWDQRWRRQTPKIEAVEYVSELKIDGSALALTYENGILVRGVTRGDGVVGEDITQNVRTIRSIPLRLNFAGAKSLEKIEVRGEVFLPLEVFKQINAERQKAGEHIFANPRNAAAGTLRQLDPRIVAQRRLDFFAYTLHISGMDDASIANTQWEALELLEKMGFPVNPNHKLCRSLTEVAQYYQSWDTKRLNLPYMTDGVVVKLNPFKLQEQLGFTQKFPRWAVALKYAAEEAPTRVENISVNVGRTGALTPLAQMRPVQLAGTTVSRATLHNSDRILQLDIRIGDTVIVRKAGEIIPEVVRVLTELRPDDTVPFIMPTHCPVCGQPVVRESGEAVTRCVNASCAAILRGEIEHWVSRDALDIKGMGEKLVHQLVDRHLVNSVADLYDLTTERLCALERMGQKSAQKLIDAIAQSKNQPWSRVLYGLGIRHVGSVNAQLLTQKYPTVEELATAKQSDIAGIYGIGAEIAQSVYQWFRIDANHVLIERLKAEGLQLAATEETTTAADVNQNLAGKTFVITGTLPSLSRDEAKALIQKAGGKVTNSVSKKTDYLLVGEDAGSKLEKAQALGITQLSEALLLEMLSN
>CP020114.1|AVZ29848.1|947300_948482_-|hypothetical-protein
MNTDKFQYSKLTHSQDIQQLGSILEQCFIMSSGDSEIYMKRLGRDKLRAIYQDQQLVGGLATIPMGQWWGGQCVPMTGIAAVGIAPEYRGSGAAIALIEKTLQELYHQEVPISVLYPATQRLYRKAGYEQGGSYCTWKISTDNIQVKELSLLLQSVDPQNYLIFQDLYQQQAKFTHGYLNRHPAIWQGLTQPDAQETVYGYLMGDKDQPQGYIIFTQERTQNGTILKVRDWTMLTKAAIQTFWSFIANHRSQIDQVQWKSSLVDSLTLLLPEQTAKITQNQRWMLRIIDVVKALEMRGYPPGISTELHLAVKDDLLPANDGKFILSVADGRGEVTKGGKGELQLDIKGLAPLYTNLFTPQQLQLTAKLQATQTALLAATQIFTASSPGMVDFF
>CP020114.1|AVZ29847.1|946235_947153_-|putative-inner-membrane-transporter-YhbE
MHHSSGRWRLGLALSLLTVFLWGILPIALTVTLQVLDVYTIIWFRFLMSFALLAAFLGVRGKLPNSQQLRSSSGKLLAIATLFLALNYFLFMQGLAFTSPANAQVLIQLSTLLLGFGGLVIFKERYTLRQWLGVSILTSGYIVFFREQLSNLITAQGTYIFGSALIALGAAAWAIYALSQKQLLLSLSSPHIMLIIYGGCALLFTPFAQPETIFTLNKFHLFILLFCALNTLIAYGAFAESLEHWEASRVSAVLALTPIITLISVALVSVFSPSLIPTEHLTLIGILGAALVVTGSMAIALGKAD
>CP020114.1|AVZ29846.1|944067_945834_-|hypothetical-protein
MNSLFDNWTSTLRRNSLLLVLAILLVILGINSSIIAAERIYATFSAIERSISVTALEKYAEDGIINKELAFYQQYLPTKQFQELQGVLLTPVKVSPDVASQFLYTPEGEFLLHRLAQVIQPESRQAQRGFHALRSALISATAEPGGLTLLNLLRKYPYSSIHIDLRRSFAIVRELEKVINQTQRAIAAVTKQSNIEAATIAKPLNLLQLADLRNPGQFHSQKHTLIFFDSKRDRLLLTDVYIPHVQSPAPVIVISHGLGTDSSNFEYLATHLASHGLAVVVPNHPGSSAKQLQTSLHKQTSQVIEPDEFINQPLDVKYVLNQLENINQSDSRFQNRLNLQEVGIFGQSLGGYTALALAGAKINFQQLAQDCTPEVLQKSWNMSLLFQCSALALNHNPSQDYNLQDDRIKAAIAVNPITSSIFGKAGLNQIKIPIMIVSSSADTVAPALYEQIQPFSWLTNSQKYLVMLLGGTHFSTIGDGNPGSQQVSLPTDLVGDASQAREYMNVLSLPFFQTYVSGNSQCLPYLNAAYTNNISRKSLGLSLVQSLNQTELAQALTPDSFKQKFPNPIVNFGFWMLNIGIAIDIERI
>CP020114.1|AVZ29845.1|943076_943949_-|tRNA-pseudouridine-synthase-B
MQGFLNLNKPFDWTSHDCVARVRKLLRLKRVGHAGTLDPAATGVLPIALGRATRLLQYLPETKAYKATIRLGVQTTTDDLQGEIISSQPCPGLNFADVQTAIAQFNGKIEQIPPIYSAIQVDGKRLYDLARRGETVEVPKRTVEIFHTEILDWRDGEFPELDVAIACGSGTYIRAIARDLGATLETGGTLAALIRTASSGFNLADSLTLTDLETQIQAGTFQPSLPDIALQHLPSVALPAISAQKWCQGQKIPQTLDFSGILRVYDQETCFLGIGKLQDSLLIPTTVLKN
>CP020114.1|AVZ29844.1|942137_942944_-|hypothetical-protein
MSKYSRFLVSLIALIIGWSLNFSHPNSIAIASPFSQVSQVFTTTPATLASFDSSLWETLLKRSYITAVEDAKTADETEISTTLQAISNNNPNLKWRDYQGQKQVLMVTWTSWNGYDNQVDLPVELSRETWVTAVPELKTFAQTLNLNPETLTLRLEQYLGLPPNNGKTKFVEMWVNASDLFRPCPDAEVNDTSCDFNFPETVEPQHQTWINNLQLVSYGDKGYPWTRLGYSYDWGSLDGEIGASEFVVRKGAKVIISSVKNTVDYSKL
>CP020114.1|AVZ29843.1|936054_936801_-|putative-L,D-transpeptidase-YnhG
MVRNESVARIFMFLCFGTAILSLAVHWRMTTTQAQFEQSASAAGSPSPGALGSGSKTAAGASTSVNKPTYQPDVQRNSVGNMSGAVSYNSAGSTELLPKMRSLFPGQRQNSQGLSSPTSSQTEVVVNLSDRRTYVYKGGEVIASYPIAIGKEGWETPTGSFRITDKQKYPIWQHPITGKVFPSGADSPLGERWIGFWSDGRNEIGFHGTPDTHLLGAAISHGCLRMRNDDVRLLYEQVNVGTLISVRD
>CP020114.1|AVZ29842.1|929394_930030_-|HTH-type-transcriptional-repressor-ComR
MARPKVGEIDRSNSADKADKIDKVEKILQGAMQEFLAHGYASTSMDRVAEASGVSKATVYSHFHDKKGLFKALIEKLARKQFYSILGSQPLKGEPYVVLRRLAATALNQMVNDQEYQSFERLLMGESARFPELSQVFIYSIAKPAIETITKYLASHPELKIPDPEATARILIGSLVHFVITQEIMHGKEIIPMESDRLIDALTHLIVNCAD
>CP020114.1|AVZ29852.1|954148_954343_-|hypothetical-protein
MMTITPSIEEIKTMIFQLPVEELITLISAIEERLETLTIMQLAETGFQEWNDPEEDIYNAIPFS
>CP020114.1|AVZ29853.1|954416_954731_-|hypothetical-protein
MKKVKMPTSDSYQDYLIESLQDPEEAGAYIEAILEAENPETELLTSALKDVIDAQLKMNHLSAQAKLKWEELNQMLLKSSGAEIYGFVGLLDALEFKLEVREKS
>CP020114.1|AVZ29854.1|954886_955960_-|toluene-4-sulfonate-monooxygenase-system-iron-sulfur-subunit-TsaM1
MSSVSKTVQNRDVRELGINPNHWYVVARSSEVTNKPINVVIWQQAMAQAPAAGIALYRDSTGKIHALEDRCPHRQVKLSHGQVIGNELECAYHGWRFNSAGECAAVPYLAENQKLPTCEIRHYPVKEQDGFIWLFPGKAEPSVEPLGLPEWEHLNYIATVSVIHTQAHYSYLIENLMDMYHGHLHQDLQAWAEASLQDIDENNYRVDAHYIAQSYYKIDKIWSISQLFFPALRKLHPEPLDVSYVYPHWVSTLGKDFKIYCLLCPISETETKAYLIHFTSLNSFWRLHKLPVWFRRFIKDSLFGAAQKLLDGLVIQDVKMIEEEQQAYLQNPQRRNYELNRALVSVQRLMRNQNIED
>CP020114.1|AVZ29855.1|964299_964833_-|hypothetical-protein
MNHQNFAALTTSSLLIVGLAVGCRSNSTLETVVPSLQPAVHANMVSRVFDAENSLALKGFDPVAYFQQGKPISGNPNFTYQWANVNWRFSTAENRDLFAKNPEKYAPQYGGFCAWAVSQGYTAPIDPNAWKIVEGKLYLNADLRIQKRWERDIPGNIKKADKNWPGLAQKIRPQNRT
>CP020114.1|AVZ29856.1|965503_965953_-|hypothetical-protein
MTPTLFGRWQTRIFLLATVGMLISLPFYGGYLGHKFDLVYFWIVFYVGLFGIGWDVLYDYLQKYMWDHDWPGVFQFYAGVVEGVVLALIIANIGLPHIPKSKFDLITFIQHYGLVWLGVYLSAWVVMRLLFPRWRFRGGEWIGKWPRIS
>CP020114.1|AVZ29857.1|965994_968037_-|hypothetical-protein
MQIKLISENKVTEEQEEQVFTLPVAIGRDITQLPAVVNGEPVSPVVLLDSSKQISRLHAQISLENNQLYLEDKSANGTELNGKKVLKERRILNSGDRLRIGNYTITVLLIQAGDPDATVVFESTSTIFNPQSEVISASSVQSSASPQSSITFNTYTGILEQQTAASSEPSGFPYSLNFWHEQRVSLGAIKSSGYLVKETEYVACGGGMGSFVWVDMLRIAGVKRDDIKVLSIQDKPYKRYETLLRNCQIPRYKRIRSGSDSCPDNIWGWPGYALRDAWRGVFSGQVSAALGFLWQVFAEPIHADTYTPRAKDVFESMDREGDRISWNKMLEHGSIRSLRQTEDGRYCIAYCASPQDEKNPQFLIAKYVHLCTGYPGIKLLPDLEKYRQEHPEETGDEKTVVQGYEPHDHIYQQLEKKGGTIFLRGSGIVASQVLDRLYAAQKIKPSIKVIHLNREPRKGNQFDLAKRHVENDWEFQPFNWPKGTWGGDMRTMLEEANPLRRRELLQGWGGTTTASRKEWRDIIQQGKKQQWYEIRYGLVTKLERNPQGGVVTHLVTNKGQQTVPADFVIDCTGLVSDPLESPFLKDLIEHYDLDLNPEGRFHVENNFEIKKVRNDRSRVYAAGIITLGGPYAPVDTFLGLQYAAHRSVEALAAVKAPGIRYIQGFYSLGQWLKWALNIKP
>CP020114.1|AVZ29858.1|970914_971796_-|lactose-transport-system-permease-protein-LacF
MPKTDTKSWLDNDTFAAWTFLSPALILLSIFIIWPIAYLFYLSFTAGSFTSTGTTWVGLRNYWRLLLTPDFWQVLGNTIYFTVATVIPSLVIPLALAVLLDKSLALRGLLRSAYFLPSIISLVAAGLGFRWLFQTDGPANAFLDFFGIAPIPWLGSTTWAMPVLIVLSIWKQLGFNMVVFLAGLQAIPPSRYEAAELDGANAWQQFWHVTLPGLQPTMIFATVTTAIFTLRSFEQVYVITGGGPLNSTNLLVYYIYQEAFAQFDFGYAAAAATVLLAFTLVFVYLQLQTWGDE
>CP020114.1|AVZ29859.1|973654_974245_-|hypothetical-protein
MVEQQNQEPEIPYLTRTQVLVAMGVTAILLWLVARVWLNYGNFYLLRWYWSSTDVLLGLGLGLIITTLSGLAYRFYPAYRQSADYYLDIVIKPLALPDLIWLGLLPALSEELLFRGVMLPALGLDNVAVIVSSLAFGILHLSGPQQWPYVVWATIIGLMLGFSALLTGNLLVPIVAHMITNWVSGCFWKFRQVKTA
>CP020114.1|AVZ29860.1|974301_975381_-|hypothetical-protein
MPIFSMQRPYTAILIIPTGVGAAIGGYAGDALPVAKVISQVCDRLITHPNVLNGASLYWNLPNTLYVEGYGLDKFASGCWGLRPVRNNKIGLLLDQGIEPELQLRHLQAADAARATLGLTLTDYVVTDAPLNVELRTSASGASWGTIGNPDSLLRAAEILINKAGAEAIAVVARFPDNMDEAAVQNYRQGQGVDPLAGAEAVISHLIVRTFKIPCAHAPALASAPPEPNLSPRSAAEELGYTFLPCVLVGLSRAPQFIINKESMTAFADDIWADQVDCAIAPANACGSSALISLNQKRCQIITVAENKTLIQVPAPALGITALQVNSYLEAVGVLVAHKAGINPAALSPQISSLQPVII
>CP020114.1|AVZ29861.1|976282_976465_-|hypothetical-protein
MTTHFITAEVDLQETPTELEKAIEAELQKQGEPLRWAITSVDEPQQKATVEAVVTTAASE

Crispr_ID: CP020114_6

• CRISPR_ID: CP020114_6
• CRISPR_location: 965199-965296
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 1 spacers

Consensus repeat of CP020114_6

Consensus_repeat	Method
AAGGCTACCGTGTACACACAAGTC	CRISPRCasFinder

spacers of CP020114_6

>6.1|965223|50|CP020114|CRISPRCasFinder
GGGAAATTCTCTTTTGTGTCATAACACCCCACCCCTAGCCCCTCCTCGCA

CRISPR arrays and Neighbor proteins around CP020114_6

CRISPR arrays

The CRISPR arrays of CP020114_6

>merge|CP020114|6|965199-965296|CRISPRCasFinder
AAGGCTACCGTGTACACACAAGTCGGGAAATTCTCTTTTGTGTCATAACACCCCACCCCTAGCCCCTCCTCGCAAGCGCTACCGTGTACACACAAGTC

>CP020114|6|6|965199-965296|CRISPRCasFinder
AAGGCTACCGTGTACACACAAGTC	GGGAAATTCTCTTTTGTGTCATAACACCCCACCCCTAGCCCCTCCTCGCA
AGCGCTACCGTGTACACACAAGTC

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
CP020114.1|AVZ29862.1|976682_977000_-|ferredoxin	unknown	unknown	gnl|CDD|273926
CP020114.1|AVZ29859.1|973654_974245_-|hypothetical-protein	unknown	unknown	gnl|CDD|376806
CP020114.1|AVZ29855.1|964299_964833_-|hypothetical-protein	unknown	unknown	unknown
CP020114.1|AVZ29854.1|954886_955960_-|toluene-4-sulfonate-monooxygenase-system-iron-sulfur-subunit-TsaM1	unknown	unknown	gnl|CDD|226985
CP020114.1|AVZ29848.1|947300_948482_-|hypothetical-protein	unknown	unknown	gnl|CDD|226925
CP020114.1|AVZ29863.1|981434_983270_-|ATP-dependent-zinc-metalloprotease-FtsH-3	unknown	unknown	gnl|CDD|273521
CP020114.1|AVZ29856.1|965503_965953_-|hypothetical-protein	unknown	unknown	unknown
CP020114.1|AVZ29849.1|948566_950603_-|DNA-ligase	unknown	unknown	gnl|CDD|236137
CP020114.1|AVZ29851.1|950887_952012_-|serine/threonine-protein-kinase-B	unknown	unknown	gnl|CDD|270916
CP020114.1|AVZ29858.1|970914_971796_-|lactose-transport-system-permease-protein-LacF	unknown	unknown	gnl|CDD|224096
CP020114.1|AVZ29847.1|946235_947153_-|putative-inner-membrane-transporter-YhbE	unknown	unknown	gnl|CDD|307170
CP020114.1|AVZ29857.1|965994_968037_-|hypothetical-protein	unknown	unknown	gnl|CDD|366133
CP020114.1|AVZ29860.1|974301_975381_-|hypothetical-protein	unknown	unknown	gnl|CDD|371736
CP020114.1|AVZ29853.1|954416_954731_-|hypothetical-protein	unknown	unknown	unknown
CP020114.1|AVZ29852.1|954148_954343_-|hypothetical-protein	unknown	unknown	unknown
CP020114.1|AVZ29864.1|983337_985221_-|arsenical-pump-driving-ATPase	unknown	unknown	gnl|CDD|223082
CP020114.1|AVZ29865.1|985263_985944_-|hypothetical-protein	unknown	unknown	gnl|CDD|377652
CP020114.1|AVZ29861.1|976282_976465_-|hypothetical-protein	unknown	unknown	unknown
CP020114.1|AVZ29846.1|944067_945834_-|hypothetical-protein	unknown	unknown	gnl|CDD|226651
CP020114.1|AVZ29850.1|950622_950805_-|hypothetical-protein	unknown	unknown	unknown

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
CP020114.1|AVZ29859.1|973654_974245_-|hypothetical-protein	gnl|CDD|376806	pfam02517, Abi, CAAX protease self-immunity. Members of this family are probably proteases (after a isoprenyl group is attached to the Cys residue in the C-terminal CAAX motif of a protein to attach it to the membrane, the AAX tripeptide being removed by one of the CAAX prenyl proteases). The family contains the CAAX prenyl protease. The proteins contain a highly conserved Glu-Glu motif at the amino end of the alignment. The alignment also contains two histidine residues that may be involved in zinc binding. While they are involved in membrane anchoring of proteins in eukaryotes, little is known about their function in prokaryotes. In some known bacteriocin loci, Abi genes have been found downstream of bacteriocin structural genes where they are probably involved in self-immunity. Investigation of the bacteriocin-like loci in the Gram positive bacteria locus from Lactobacillus sakei 23K confirmed that the bacteriocin-like genes (sak23Kalphabeta) exhibited antimicrobial activity when expressed in a heterologous host and that the associated Abi gene (sak23Ki) conferred immunity against the cognate bacteriocin. Interestingly, the immunity genes from three similar systems conferred a high degree of cross-immunity against each other's bacteriocins, suggesting the recognition of a common receptor. Site-directed mutagenesis demonstrated that the conserved motifs constituting the putative proteolytic active site of the Abi proteins are essential for the immunity function of Sak23Ki - thus a new concept in self-immunity.	5.26214e-14
CP020114.1|AVZ29858.1|970914_971796_-|lactose-transport-system-permease-protein-LacF	gnl|CDD|224096	COG1175, UgpA, ABC-type sugar transport systems, permease components [Carbohydrate transport and metabolism].	2.68024e-89
CP020114.1|AVZ29854.1|954886_955960_-|toluene-4-sulfonate-monooxygenase-system-iron-sulfur-subunit-TsaM1	gnl|CDD|226985	COG4638, HcaE, Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit [Inorganic ion transport and metabolism / General function prediction only].	1.5612e-66
CP020114.1|AVZ29848.1|947300_948482_-|hypothetical-protein	gnl|CDD|226925	COG4552, Eis, Predicted acetyltransferase involved in intracellular survival and related acetyltransferases [General function prediction only].	4.0003e-114
CP020114.1|AVZ29863.1|981434_983270_-|ATP-dependent-zinc-metalloprotease-FtsH-3	gnl|CDD|273521	TIGR01243, Cell_division_cycle_protein_48_homolog_MJ1156, AAA family ATPase, CDC48 subfamily. This subfamily of the AAA family ATPases includes two members each from three archaeal species. It also includes yeast CDC48 (cell division control protein 48) and the human ortholog, transitional endoplasmic reticulum ATPase (valosin-containing protein). These proteins in eukaryotes are involved in the budding and transfer of membrane from the transitional endoplasmic reticulum to the Golgi apparatus.	0
CP020114.1|AVZ29864.1|983337_985221_-|arsenical-pump-driving-ATPase	gnl|CDD|223082	COG0003, ArsA, Predicted ATPase involved in chromosome partitioning [Cell division and chromosome partitioning].	1.72137e-54
CP020114.1|AVZ29851.1|950887_952012_-|serine/threonine-protein-kinase-B	gnl|CDD|270916	cd14014, STKc_PknB_like, Catalytic domain of bacterial Serine/Threonine kinases, PknB and similar proteins. STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. This subfamily includes many bacterial eukaryotic-type STKs including Staphylococcus aureus PknB (also called PrkC or Stk1), Bacillus subtilis PrkC, and Mycobacterium tuberculosis Pkn proteins (PknB, PknD, PknE, PknF, PknL, and PknH), among others. S. aureus PknB is the only eukaryotic-type STK present in this species, although many microorganisms encode for several such proteins. It is important for the survival and pathogenesis of S. aureus as it is involved in the regulation of purine and pyrimidine biosynthesis, cell wall metabolism, autolysis, virulence, and antibiotic resistance. M. tuberculosis PknB is essential for growth and it acts on diverse substrates including proteins involved in peptidoglycan synthesis, cell division, transcription, stress responses, and metabolic regulation. B. subtilis PrkC is located at the inner membrane of endospores and functions to trigger spore germination. Bacterial STKs in this subfamily show varied domain architectures. The well-characterized members such as S. aureus and M. tuberculosis PknB, and B. subtilis PrkC, contain an N-terminal cytosolic kinase domain, a transmembrane (TM) segment, and mutliple C-terminal extracellular PASTA domains. The PknB subfamily is part of a larger superfamily that includes the catalytic domains of other protein STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase.	1.07637e-74
CP020114.1|AVZ29862.1|976682_977000_-|ferredoxin	gnl|CDD|273926	TIGR02008, Ferredoxin_root_R-B1, ferredoxin [2Fe-2S]. This model represents single domain 2Fe-2S (also called plant type) ferredoxins. In general, these occur as a single domain proteins or with a chloroplast transit peptide. Species tend to be photosynthetic, but several forms may occur in one species and individually may not be associated with photocynthesis. Halobacterial forms differ somewhat in architecture; they score between trusted and noise cutoffs. Sequences scoring below the noise cutoff tend to be ferredoxin-related domains of larger proteins.	7.78782e-28
CP020114.1|AVZ29847.1|946235_947153_-|putative-inner-membrane-transporter-YhbE	gnl|CDD|307170	pfam00892, EamA, EamA-like transporter family. This family includes many hypothetical membrane proteins of unknown function. Many of the proteins contain two copies of the aligned region. The family used to be known as DUF6. Members of this family usually carry 5+5 transmembrane domains, and this domain attempts to model five of these.	1.45434e-06
CP020114.1|AVZ29857.1|965994_968037_-|hypothetical-protein	gnl|CDD|366133	pfam00498, FHA, FHA domain. The FHA (Forkhead-associated) domain is a phosphopeptide binding motif.	1.05663e-11
CP020114.1|AVZ29860.1|974301_975381_-|hypothetical-protein	gnl|CDD|371736	pfam11805, DUF3326, Protein of unknown function (DUF3326). This protein is functionally uncharacterized. It is about 300-500 amino acids in length. This family is found in plants and bacteria.	0
CP020114.1|AVZ29849.1|948566_950603_-|DNA-ligase	gnl|CDD|236137	PRK07956, ligA, NAD-dependent DNA ligase LigA; Validated.	0
CP020114.1|AVZ29865.1|985263_985944_-|hypothetical-protein	gnl|CDD|377652	pfam06386, GvpL_GvpF, Gas vesicle synthesis protein GvpL/GvpF. This family consists of several bacterial and archaeal gas vesicle synthesis protein (GvpL/GvpF) sequences. The exact function of this family is unknown.	8.38757e-20
CP020114.1|AVZ29846.1|944067_945834_-|hypothetical-protein	gnl|CDD|226651	COG4188, COG4188, Predicted dienelactone hydrolase [General function prediction only].	2.23994e-74

Protein sequences

>CP020114.1|AVZ29855.1|964299_964833_-|hypothetical-protein
MNHQNFAALTTSSLLIVGLAVGCRSNSTLETVVPSLQPAVHANMVSRVFDAENSLALKGFDPVAYFQQGKPISGNPNFTYQWANVNWRFSTAENRDLFAKNPEKYAPQYGGFCAWAVSQGYTAPIDPNAWKIVEGKLYLNADLRIQKRWERDIPGNIKKADKNWPGLAQKIRPQNRT
>CP020114.1|AVZ29854.1|954886_955960_-|toluene-4-sulfonate-monooxygenase-system-iron-sulfur-subunit-TsaM1
MSSVSKTVQNRDVRELGINPNHWYVVARSSEVTNKPINVVIWQQAMAQAPAAGIALYRDSTGKIHALEDRCPHRQVKLSHGQVIGNELECAYHGWRFNSAGECAAVPYLAENQKLPTCEIRHYPVKEQDGFIWLFPGKAEPSVEPLGLPEWEHLNYIATVSVIHTQAHYSYLIENLMDMYHGHLHQDLQAWAEASLQDIDENNYRVDAHYIAQSYYKIDKIWSISQLFFPALRKLHPEPLDVSYVYPHWVSTLGKDFKIYCLLCPISETETKAYLIHFTSLNSFWRLHKLPVWFRRFIKDSLFGAAQKLLDGLVIQDVKMIEEEQQAYLQNPQRRNYELNRALVSVQRLMRNQNIED
>CP020114.1|AVZ29853.1|954416_954731_-|hypothetical-protein
MKKVKMPTSDSYQDYLIESLQDPEEAGAYIEAILEAENPETELLTSALKDVIDAQLKMNHLSAQAKLKWEELNQMLLKSSGAEIYGFVGLLDALEFKLEVREKS
>CP020114.1|AVZ29852.1|954148_954343_-|hypothetical-protein
MMTITPSIEEIKTMIFQLPVEELITLISAIEERLETLTIMQLAETGFQEWNDPEEDIYNAIPFS
>CP020114.1|AVZ29851.1|950887_952012_-|serine/threonine-protein-kinase-B
MSYCLNSHCLQPENAADVKFCLSCGSKLLLKDRYRAIKPIGQGGFGKTFLAVDEDKPSKPPCVIKQFYPQAQGTNSVQKAVELFTLEAVQLDDLGQHPQIPSLLAYFTQDDRQYLVQEFIDGQNLAEELAQKGAFSEAQIWQLLKDLLPVLQFCHSRGVIHRDIKPENILRDRNAKLVLVDFGASKSATGTALNQTGTSIGTPEYVAPEQMRGRALFASDIYSLGATCVHLLTARSPFDSYDINHDTWIWQKFLQTPLSSGLNRILEKMLVSIPMRRYQTADEVLKDLNQSPVVATPATTAKPQSTPTSVSKSSSKPDLELEEMKTQFLGIGKPQPNQPQPPKPISPPANNSVIDKELEELKAKFMGNNNSSSQ
>CP020114.1|AVZ29850.1|950622_950805_-|hypothetical-protein
MNTDDFFVGFIRSVLFVTDVRVKLKWVYLASIAIVNFNSADRSLTRMKPSNLGKLSLSIC
>CP020114.1|AVZ29849.1|948566_950603_-|DNA-ligase
MIQSQSEVKRVEELRQLLQQASYAYYVLDAPMMEDAVYDQLYRELQELEIKYPEFITPDSPTQRVGEKPATHFTSVRHNIPLYSLENAFNIDELQIWDQRWRRQTPKIEAVEYVSELKIDGSALALTYENGILVRGVTRGDGVVGEDITQNVRTIRSIPLRLNFAGAKSLEKIEVRGEVFLPLEVFKQINAERQKAGEHIFANPRNAAAGTLRQLDPRIVAQRRLDFFAYTLHISGMDDASIANTQWEALELLEKMGFPVNPNHKLCRSLTEVAQYYQSWDTKRLNLPYMTDGVVVKLNPFKLQEQLGFTQKFPRWAVALKYAAEEAPTRVENISVNVGRTGALTPLAQMRPVQLAGTTVSRATLHNSDRILQLDIRIGDTVIVRKAGEIIPEVVRVLTELRPDDTVPFIMPTHCPVCGQPVVRESGEAVTRCVNASCAAILRGEIEHWVSRDALDIKGMGEKLVHQLVDRHLVNSVADLYDLTTERLCALERMGQKSAQKLIDAIAQSKNQPWSRVLYGLGIRHVGSVNAQLLTQKYPTVEELATAKQSDIAGIYGIGAEIAQSVYQWFRIDANHVLIERLKAEGLQLAATEETTTAADVNQNLAGKTFVITGTLPSLSRDEAKALIQKAGGKVTNSVSKKTDYLLVGEDAGSKLEKAQALGITQLSEALLLEMLSN
>CP020114.1|AVZ29848.1|947300_948482_-|hypothetical-protein
MNTDKFQYSKLTHSQDIQQLGSILEQCFIMSSGDSEIYMKRLGRDKLRAIYQDQQLVGGLATIPMGQWWGGQCVPMTGIAAVGIAPEYRGSGAAIALIEKTLQELYHQEVPISVLYPATQRLYRKAGYEQGGSYCTWKISTDNIQVKELSLLLQSVDPQNYLIFQDLYQQQAKFTHGYLNRHPAIWQGLTQPDAQETVYGYLMGDKDQPQGYIIFTQERTQNGTILKVRDWTMLTKAAIQTFWSFIANHRSQIDQVQWKSSLVDSLTLLLPEQTAKITQNQRWMLRIIDVVKALEMRGYPPGISTELHLAVKDDLLPANDGKFILSVADGRGEVTKGGKGELQLDIKGLAPLYTNLFTPQQLQLTAKLQATQTALLAATQIFTASSPGMVDFF
>CP020114.1|AVZ29847.1|946235_947153_-|putative-inner-membrane-transporter-YhbE
MHHSSGRWRLGLALSLLTVFLWGILPIALTVTLQVLDVYTIIWFRFLMSFALLAAFLGVRGKLPNSQQLRSSSGKLLAIATLFLALNYFLFMQGLAFTSPANAQVLIQLSTLLLGFGGLVIFKERYTLRQWLGVSILTSGYIVFFREQLSNLITAQGTYIFGSALIALGAAAWAIYALSQKQLLLSLSSPHIMLIIYGGCALLFTPFAQPETIFTLNKFHLFILLFCALNTLIAYGAFAESLEHWEASRVSAVLALTPIITLISVALVSVFSPSLIPTEHLTLIGILGAALVVTGSMAIALGKAD
>CP020114.1|AVZ29846.1|944067_945834_-|hypothetical-protein
MNSLFDNWTSTLRRNSLLLVLAILLVILGINSSIIAAERIYATFSAIERSISVTALEKYAEDGIINKELAFYQQYLPTKQFQELQGVLLTPVKVSPDVASQFLYTPEGEFLLHRLAQVIQPESRQAQRGFHALRSALISATAEPGGLTLLNLLRKYPYSSIHIDLRRSFAIVRELEKVINQTQRAIAAVTKQSNIEAATIAKPLNLLQLADLRNPGQFHSQKHTLIFFDSKRDRLLLTDVYIPHVQSPAPVIVISHGLGTDSSNFEYLATHLASHGLAVVVPNHPGSSAKQLQTSLHKQTSQVIEPDEFINQPLDVKYVLNQLENINQSDSRFQNRLNLQEVGIFGQSLGGYTALALAGAKINFQQLAQDCTPEVLQKSWNMSLLFQCSALALNHNPSQDYNLQDDRIKAAIAVNPITSSIFGKAGLNQIKIPIMIVSSSADTVAPALYEQIQPFSWLTNSQKYLVMLLGGTHFSTIGDGNPGSQQVSLPTDLVGDASQAREYMNVLSLPFFQTYVSGNSQCLPYLNAAYTNNISRKSLGLSLVQSLNQTELAQALTPDSFKQKFPNPIVNFGFWMLNIGIAIDIERI
>CP020114.1|AVZ29856.1|965503_965953_-|hypothetical-protein
MTPTLFGRWQTRIFLLATVGMLISLPFYGGYLGHKFDLVYFWIVFYVGLFGIGWDVLYDYLQKYMWDHDWPGVFQFYAGVVEGVVLALIIANIGLPHIPKSKFDLITFIQHYGLVWLGVYLSAWVVMRLLFPRWRFRGGEWIGKWPRIS
>CP020114.1|AVZ29857.1|965994_968037_-|hypothetical-protein
MQIKLISENKVTEEQEEQVFTLPVAIGRDITQLPAVVNGEPVSPVVLLDSSKQISRLHAQISLENNQLYLEDKSANGTELNGKKVLKERRILNSGDRLRIGNYTITVLLIQAGDPDATVVFESTSTIFNPQSEVISASSVQSSASPQSSITFNTYTGILEQQTAASSEPSGFPYSLNFWHEQRVSLGAIKSSGYLVKETEYVACGGGMGSFVWVDMLRIAGVKRDDIKVLSIQDKPYKRYETLLRNCQIPRYKRIRSGSDSCPDNIWGWPGYALRDAWRGVFSGQVSAALGFLWQVFAEPIHADTYTPRAKDVFESMDREGDRISWNKMLEHGSIRSLRQTEDGRYCIAYCASPQDEKNPQFLIAKYVHLCTGYPGIKLLPDLEKYRQEHPEETGDEKTVVQGYEPHDHIYQQLEKKGGTIFLRGSGIVASQVLDRLYAAQKIKPSIKVIHLNREPRKGNQFDLAKRHVENDWEFQPFNWPKGTWGGDMRTMLEEANPLRRRELLQGWGGTTTASRKEWRDIIQQGKKQQWYEIRYGLVTKLERNPQGGVVTHLVTNKGQQTVPADFVIDCTGLVSDPLESPFLKDLIEHYDLDLNPEGRFHVENNFEIKKVRNDRSRVYAAGIITLGGPYAPVDTFLGLQYAAHRSVEALAAVKAPGIRYIQGFYSLGQWLKWALNIKP
>CP020114.1|AVZ29858.1|970914_971796_-|lactose-transport-system-permease-protein-LacF
MPKTDTKSWLDNDTFAAWTFLSPALILLSIFIIWPIAYLFYLSFTAGSFTSTGTTWVGLRNYWRLLLTPDFWQVLGNTIYFTVATVIPSLVIPLALAVLLDKSLALRGLLRSAYFLPSIISLVAAGLGFRWLFQTDGPANAFLDFFGIAPIPWLGSTTWAMPVLIVLSIWKQLGFNMVVFLAGLQAIPPSRYEAAELDGANAWQQFWHVTLPGLQPTMIFATVTTAIFTLRSFEQVYVITGGGPLNSTNLLVYYIYQEAFAQFDFGYAAAAATVLLAFTLVFVYLQLQTWGDE
>CP020114.1|AVZ29859.1|973654_974245_-|hypothetical-protein
MVEQQNQEPEIPYLTRTQVLVAMGVTAILLWLVARVWLNYGNFYLLRWYWSSTDVLLGLGLGLIITTLSGLAYRFYPAYRQSADYYLDIVIKPLALPDLIWLGLLPALSEELLFRGVMLPALGLDNVAVIVSSLAFGILHLSGPQQWPYVVWATIIGLMLGFSALLTGNLLVPIVAHMITNWVSGCFWKFRQVKTA
>CP020114.1|AVZ29860.1|974301_975381_-|hypothetical-protein
MPIFSMQRPYTAILIIPTGVGAAIGGYAGDALPVAKVISQVCDRLITHPNVLNGASLYWNLPNTLYVEGYGLDKFASGCWGLRPVRNNKIGLLLDQGIEPELQLRHLQAADAARATLGLTLTDYVVTDAPLNVELRTSASGASWGTIGNPDSLLRAAEILINKAGAEAIAVVARFPDNMDEAAVQNYRQGQGVDPLAGAEAVISHLIVRTFKIPCAHAPALASAPPEPNLSPRSAAEELGYTFLPCVLVGLSRAPQFIINKESMTAFADDIWADQVDCAIAPANACGSSALISLNQKRCQIITVAENKTLIQVPAPALGITALQVNSYLEAVGVLVAHKAGINPAALSPQISSLQPVII
>CP020114.1|AVZ29861.1|976282_976465_-|hypothetical-protein
MTTHFITAEVDLQETPTELEKAIEAELQKQGEPLRWAITSVDEPQQKATVEAVVTTAASE
>CP020114.1|AVZ29862.1|976682_977000_-|ferredoxin
MSNIYTVEINHQGKIYTLQVPEDETILSVAETAGLGFPNSCNAGVCTTCAGLITEGTVDQSDCMGVSSELQEQGYALLCVSYPRSDLKIETEKEDVVYQLQFGKG
>CP020114.1|AVZ29863.1|981434_983270_-|ATP-dependent-zinc-metalloprotease-FtsH-3
MSDLFKGFEQFIELVKTLEEKVENGEIKTDVQFNSRSLSSIPRNGNIPRTNNNTTSNVGTTSIRTPSSPARDTGASDPNVQPEGNLGITLKDIGGLNQELKELKELIAIPLKRPDLLVKLGLEPTHGVLLVGPSGTGKTLTARALAEELGVNYIALVGPEVITKYYGEAEQKLRAIFEKAAKNAPCIIFIDEIDSLAPDRSAVEGEVEKRLVAQLLSLMDGFSQNKGVILLGATNRPDHLDPALRRPGRFDREIQFRVPDINGRKEILEVLTRAMPLDNTVDLEFIADRTVGFVGADLKAVCQKAAYTALRRQVPSIDMPIPEVITVQQSDFLQALKEIKPAVLRSMEVEVPHVEWDDIGGLETIKQTLRESVEGALLYPELYKETKARAPKGILLWGPPGTGKTLLAKAVASQARANFIGINGPDLLSRWVGASEQAVRELFAKARQADPCVIFIDELDTLAPARGTYTGDSGVSNRVVGQLLTELDGLGTGANILVIGATNRPDAIDPALLRAGRLDLQLKVDLPNLDSRCKILQVYNQGRPILNVDLEHWAKVTEGWNGADLVLLCNQAAVEAIRRFRSQGETDTAAIKITVDDFQASYEALIQQRTV
>CP020114.1|AVZ29864.1|983337_985221_-|arsenical-pump-driving-ATPase
MKQYDSLNLVMFSGKGGVGKTTISCCFARYWATKFPEEKILLLSTDPAHSLGDILLSKVTDDGSQVEDLPNLSVRALDAEKLLVEFRAKYSRLLELLVERGSLADGEDLAPVWELNWPGLNELMGLLEIQRLLSEKTVDRIVLDMAPSGHTLNLLQLEDFFDVILNSFELFQQKHRVITESFRGSYKPDEVDDFLEHMKSQLAEGRRLLQDETFTGCLVVTIAEPMCLSETERFLDNLKTLNIHYTGILINRIITDSDINTDRYAEQQNLTDKFLKIAGNQPVFIVPQQAKEPLGPLALDDLARQIQKIDSVELSAPPAIQWPSRILPSFSDFVAEGCQLIIVGGKGGVGKTTVAGALGWGLANRHPQQKIRIISIDPAHSLGDAFGQKLGHQASSLATNLTGQEIDADEILDQFRTDYLWELADMISGEGTETDSTIDIAYLPGAWRQIMSQALPGIDEILSLITIMDLLDSNQQDLIILDTAPTGHLLQFLAMPSALGDWLSWIFKLWIKYQNVVGRLDLIGRLRNLRQQVVQAQKKLKNPKHTQFVGVIQAEDAIISEHIRLTASLKDMGIQQRYVVQNRYSQEIGIDPGLFPEQTIIRLPNLPRSVEPIARIQAAANLLFDFD
>CP020114.1|AVZ29865.1|985263_985944_-|hypothetical-protein
MESENLYSYAFLETPSLPLILPQGAASQVVLIKSTNLSAIVEPGISLESFQDNEQKIIEMALCHDRVICELFQQITVLPVQFGICFNSQKNLLIHLESNTEKYRQQLQKIHGKTEFTLKLIPVILEEVAPVKSRGKDYFLAKKQQYQNQKDFSLAQAAEKEYLIDLITTTNNYPVVVQEKEQEVSIHMLVNIEDRNCFLEQTLNWQKACPRWNLLLGDSLPPYHFI

Crispr_ID: CP020114_8

• CRISPR_ID: CP020114_8
• CRISPR_location: 1521840-1521967
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 1 spacers

Consensus repeat of CP020114_8

Consensus_repeat	Method
CGCGGTAAGCGAAGCTATGCCGTAGGCTTTACGTG	CRISPRCasFinder

spacers of CP020114_8

>8.1|1521875|58|CP020114|CRISPRCasFinder
TCCTTCTCCCAAAGGGAGAGGCTAGCGCCAACGTCCCTTCTCCCAAAGGGAGACGCTT

CRISPR arrays and Neighbor proteins around CP020114_8

CRISPR arrays

The CRISPR arrays of CP020114_8

>merge|CP020114|8|1521840-1521967|CRISPRCasFinder
CGCGGTAAGCGAAGCTATGCCGTAGGCTTTACGTGTCCTTCTCCCAAAGGGAGAGGCTAGCGCCAACGTCCCTTCTCCCAAAGGGAGACGCTTCGCGGTAAGCGAAGCTATGCCGAAGGCTTTACGTG

>CP020114|8|8|1521840-1521967|CRISPRCasFinder
CGCGGTAAGCGAAGCTATGCCGTAGGCTTTACGTG	TCCTTCTCCCAAAGGGAGAGGCTAGCGCCAACGTCCCTTCTCCCAAAGGGAGACGCTT
CGCGGTAAGCGAAGCTATGCCGAAGGCTTTACGTG

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
CP020114.1|AVZ30083.1|1518192_1518537_-|hypothetical-protein	unknown	unknown	unknown
CP020114.1|AVZ30092.1|1554568_1555861_-|hypothetical-protein	unknown	unknown	gnl|CDD|226984
CP020114.1|AVZ30081.1|1517107_1517299_-|hypothetical-protein	unknown	unknown	unknown
CP020114.1|AVZ30085.1|1537610_1538711_-|precorrin-8X/cobalt-precorrin-8-methylmutase	unknown	unknown	gnl|CDD|224993
CP020114.1|AVZ30091.1|1553916_1554564_-|hypothetical-protein	unknown	unknown	gnl|CDD|379470
CP020114.1|AVZ30078.1|1514145_1515201_-|hypothetical-protein	unknown	unknown	gnl|CDD|226346
CP020114.1|AVZ30080.1|1516271_1516949_-|hypothetical-protein	unknown	unknown	unknown
CP020114.1|AVZ30084.1|1528272_1531143_-|phosphate-binding-protein-PstS	unknown	unknown	gnl|CDD|270371
CP020114.1|AVZ30086.1|1538746_1540291_-|hypothetical-protein	unknown	unknown	gnl|CDD|372986
CP020114.1|AVZ30088.1|1543614_1543923_-|hypothetical-protein	unknown	unknown	unknown
CP020114.1|AVZ30087.1|1543342_1543627_-|hypothetical-protein	unknown	unknown	unknown
CP020114.1|AVZ30074.1|1509218_1510502_-|valine--pyruvate-aminotransferase	unknown	unknown	gnl|CDD|236517
CP020114.1|AVZ30082.1|1517323_1518193_-|hypothetical-protein	unknown	unknown	gnl|CDD|379142
CP020114.1|AVZ30093.1|1556208_1556595_-|steroid-delta-isomerase	unknown	unknown	gnl|CDD|238404
CP020114.1|AVZ30090.1|1553055_1553835_-|hypothetical-protein	unknown	unknown	gnl|CDD|316044
CP020114.1|AVZ30075.1|1511065_1511275_-|hypothetical-protein	unknown	unknown	unknown
CP020114.1|AVZ30079.1|1515197_1516151_-|hypothetical-protein	unknown	unknown	gnl|CDD|223786
CP020114.1|AVZ30077.1|1511701_1513216_-|serine/threonine-protein-kinase-pkn1	unknown	unknown	gnl|CDD|224182
CP020114.1|AVZ30076.1|1511320_1511590_-|hypothetical-protein	unknown	unknown	gnl|CDD|380307
CP020114.1|AVZ30089.1|1545287_1548182_-|putative-phycocyanin-operon-protein-Z	unknown	unknown	gnl|CDD|224331

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
CP020114.1|AVZ30092.1|1554568_1555861_-|hypothetical-protein	gnl|CDD|226984	COG4637, COG4637, Predicted ATPase [General function prediction only].	1.39572e-51
CP020114.1|AVZ30085.1|1537610_1538711_-|precorrin-8X/cobalt-precorrin-8-methylmutase	gnl|CDD|224993	COG2082, CobH, Precorrin isomerase [Coenzyme metabolism].	7.193e-73
CP020114.1|AVZ30091.1|1553916_1554564_-|hypothetical-protein	gnl|CDD|379470	pfam14103, DUF4276, Domain of unknown function (DUF4276). This family of proteins is functionally uncharacterized. This family of proteins is found in bacteria and archaea. Proteins in this family are typically between 190 and 224 amino acids in length. There is a single completely conserved residue E that may be functionally important.	1.6811e-05
CP020114.1|AVZ30089.1|1545287_1548182_-|putative-phycocyanin-operon-protein-Z	gnl|CDD|224331	COG1413, COG1413, FOG: HEAT repeat [Energy production and conversion].	5.54353e-42
CP020114.1|AVZ30077.1|1511701_1513216_-|serine/threonine-protein-kinase-pkn1	gnl|CDD|224182	COG1262, COG1262, Uncharacterized conserved protein [Function unknown].	2.84771e-89
CP020114.1|AVZ30086.1|1538746_1540291_-|hypothetical-protein	gnl|CDD|372986	pfam14264, Glucos_trans_II, Glucosyl transferase GtrII. This family includes glucosyl transferase II from the Shigella phage SfII, which mediates seroconversion of S. flexneri when the phage is integrated into the host chromosome.	0.00357487
CP020114.1|AVZ30074.1|1509218_1510502_-|valine--pyruvate-aminotransferase	gnl|CDD|236517	PRK09440, avtA, valine--pyruvate transaminase; Provisional.	0
CP020114.1|AVZ30082.1|1517323_1518193_-|hypothetical-protein	gnl|CDD|379142	pfam13365, Trypsin_2, Trypsin-like peptidase domain. This family includes trypsin-like peptidase domains.	1.61398e-14
CP020114.1|AVZ30093.1|1556208_1556595_-|steroid-delta-isomerase	gnl|CDD|238404	cd00781, ketosteroid_isomerase, ketosteroid isomerase: Many biological reactions proceed by enzymatic cleavage of a C-H bond adjacent to carbonyl or a carboxyl group, leading to an enol or a enolate intermediate that is subsequently re-protonated at the same or an adjacent carbon. Ketosteroid isomerases are important members of this class of enzymes which are the most proficient of all enzymes known and have served as a paradigm for enzymatic enolizations since its discovery in 1954. This CD includes members of this class that calalyze the isomerization of various beta,gamma-unsaturated isomers at nearly a diffusion-controlled rate. These enzymes are widely distributed in bacteria.	6.00256e-21
CP020114.1|AVZ30090.1|1553055_1553835_-|hypothetical-protein	gnl|CDD|316044	pfam13483, Lactamase_B_3, Beta-lactamase superfamily domain. This family is part of the beta-lactamase superfamily and is related to pfam00753.	1.18727e-41
CP020114.1|AVZ30084.1|1528272_1531143_-|phosphate-binding-protein-PstS	gnl|CDD|270371	cd13653, PBP2_phosphate_like_1, Substrate binding domain of putative ABC-type phosphate transporter, a member of the type 2 periplasmic binding fold superfamily. This subfamily contains uncharacterized phosphate binding domains found in PstS proteins that serve as initial receptors in the ABC transport of phosphate in eubacteria and archaea. After binding the ligand, PstS interacts with a cognate membrane transport complex comprised of two integral membrane domains and two cytoplasmically located ATPase domains. This interaction triggers the ligand translocation across the cytoplasmic membrane energized by ATP hydrolysis. The PstS proteins belong to the PBP2 superfamily of periplasmic binding proteins that differ in size and ligand specificity, but have similar tertiary structures consisting of two globular subdomains connected by a flexible hinge. They have been shown to bind their ligand in the cleft between these domains in a manner resembling a Venus flytrap.	9.62558e-47
CP020114.1|AVZ30079.1|1515197_1516151_-|hypothetical-protein	gnl|CDD|223786	COG0714, COG0714, MoxR-like ATPases [General function prediction only].	3.85837e-32
CP020114.1|AVZ30078.1|1514145_1515201_-|hypothetical-protein	gnl|CDD|226346	COG3825, COG3825, Uncharacterized protein conserved in bacteria [Function unknown].	3.19503e-18
CP020114.1|AVZ30076.1|1511320_1511590_-|hypothetical-protein	gnl|CDD|380307	NF033474, DivGenRetAVD, diversity-generating retroelement protein Avd. Avd (accessory variability determinant) is part of diversity-generating retroelement (GDR) system through which a portion of a protein-coding gene can be rewritten, creating diversity that can affect host range. The founding member of this family, bAvd, from a Bordetella bacteriophage, belongs to a retrohoming element called BPP-1. Members of this family are four-helix bundle proteins, related to those of family TIGR02436, some of whose members are found in long intervening sequence (IVS) regions in 23S rRNA.	2.30412e-31

Protein sequences

>CP020114.1|AVZ30083.1|1518192_1518537_-|hypothetical-protein
MKEKKIAEFSLADGTKFLVEVDEPEGRGIERVALPSGRQVLKAQQTFEEALENIKPVASTIISKLRDLNQPADEVEVKFGLKLTADAGAIFASVGGEVSYEITLKWSSKGQGNG
>CP020114.1|AVZ30082.1|1517323_1518193_-|hypothetical-protein
MNAIDFNSHKNSIIRIFYQKDVVGIGFLVAEKYALTCAHVVGEALLISSSIANIPDGEIQVDFPLANSTEKIKATVVYWHPTSDLNSDVLIEDIAVLKLEHLPSQAQVTKLILSENLSKHPFKVFGCPQGVAFGVWATGVLSEQNAKQWVQLEDTKVTGYAIAKGFSGSPVWDEELQGVVGMVVAADTRREAARASFMIPNQTLVKAWQYLKSVVMGSKPLEKPVKPALSSMRRKYLETQKKDLEEKIDGITTEMGLLDPASMAYKNLKKRNELYFSELEKIEEQLTGY
>CP020114.1|AVZ30081.1|1517107_1517299_-|hypothetical-protein
MNRRESLETQKKDLEEKIDGITTEMGLLDPALMAYNNLKKKMNYIFLILKKLKVNLVNFGNHH
>CP020114.1|AVZ30080.1|1516271_1516949_-|hypothetical-protein
MIRRTHNSLTKELDDKSDKTYHYHHHHIKHFQINLLDNYITNEETLLDAIAQFIPNTKKDENLDNYASNIIDTLEGSLQTGSIIFFELDCWDALGENQDSLLSWFVEKFWTPLTKKHNEISQKYPNVKILIFMTTGSSLSQEMKCKPYFCTPSQFCHQKIVKLPLPKKWEQKDITHWIEDTYQYSPLDVINGSTSITESTKIFTLSQGDPMKTCHFLERRFSKMA
>CP020114.1|AVZ30079.1|1515197_1516151_-|hypothetical-protein
MNPFQCDPKARPKNPHPDSPTELEPYIADSALIEAVNLAIFLQRPLLIEGEAGCGKTRLAVAIAYELGLPFYRWDIRSTTKVQEGLYEYDAILRLHDVQTQNLSPSINPLTKKPRNPQEPQDYRELGSLGKAFQSHEYPAVLLIDEIDKADVDFPNDLLSILEKPWKFFIRETGEEIKANPEKLPIIIITSNKEKGSLPTPFLRRCLYHYVRFPNQPEELQKIVNAHYTQKQKVDKTTIPKADLVKEAIAKFLKIREDKSLFKLPGTSEFLDWLAALDHFTPKPYPVTQLQQEKTIPYRDLLFKLRQDWQNPNYPSV
>CP020114.1|AVZ30078.1|1514145_1515201_-|hypothetical-protein
MSDFNPHPLLDPLFYRLRRDGFALGVSEYLVTLKAIDGGWGTEDANALKKLLKLLWCHSLAQQDQLEVVFRSISAELAPKKEELRENNPDSSSELTLSSNPSPPSSETQVSTVDSFPTPTPEFSLLPVKSPISLLEQQTEDRDFQTYAPISRRYMVYSWRYLRRMVGDGREDILDVAATIAQVCEQGFFLAPKYGRRERNHVDLLLLIDQEGSMTPFHHFTRDLVQTAQYESNIQQVRVGYFHNVPAEYIYRDPYLTEKVLLESTLAAGDGNTSILIVSDGGAARGDRRSQRFSATAEVLWQIKQHTKLIAWLNPIPPERWRGTTAQFIANLLPMYPLDPHGLNQAIAEIR
>CP020114.1|AVZ30077.1|1511701_1513216_-|serine/threonine-protein-kinase-pkn1
MNTPLSDELIQEIAEQQINRFVGRFEPSYEYLAAHCALPLVLTPELVNYIRVQFLLNEEVPWIAEADLLLSDLCRPVGYELYAMTPVVRSNLLAKFTQEPFQDKFGRNRLKSIAQLLLDYVTYLMRTHPHKPDQLQAQQWAAMVYLDEQRTQAEEEIRNALQALVEGGKNGQAELARLQRLITEFQPQLSQYDSLRELAEEVGRLLKGEIPSRPMVFEYQTPTVNRRGEIINTETKTAQYFTENLPNNVTLEMVSIPGGKFLMGSPKNEDQSSNTQRPQHEVTVPPFFMGKYPITQAQWKAVAALAKVNKDLDANPSKFKGEQRPVERVSWYDAVEFCQRLTAHTKREYRLPSEAEWEYACRAGTTTPFHFGETITSELANYNATSIYGRGVKGTYRQETTLVGSFNAANNFGLYDMHGNVWEWCLDDWHDNYEGAPIDGSAWFNDNDNLSQKQGKALLRGGSWINSPGNCRSASRDNDHWAERDYIYNFVGFRVVCAVGWILQ
>CP020114.1|AVZ30076.1|1511320_1511590_-|hypothetical-protein
MKELSVIQKTYNCIKWYARIIERLPKIHKFTLGDRMSNQFYELLEGLIKAKYAKDKLTQLEALNTQLDILRYQTRMLLDFNKMSIAVKV
>CP020114.1|AVZ30075.1|1511065_1511275_-|hypothetical-protein
MTIKVINEQENLKEVIEVLLTHLEPSKVMKFWAACQLGKGDYLQLKENLFAQEDVESLYKKIKAYQDRE
>CP020114.1|AVZ30074.1|1509218_1510502_-|valine--pyruvate-aminotransferase
MNPALTKIGAQMSNLTGVRAIMKDIVETLKAGAGQEFMNLSAGNPLILPEVEQLWRDCTAELLASSEYGEVVCRYGSSQGYAPLIEAIANDFNKRYGLNLSDRNILITPGSQSLYFYAANAFGGYTNNGELKQIVLPLSPDYTGYGGVCLEQEALKAYKPTLDIDAAAHRFKYRPDFSQLSITESTGCVLFSRPCNPTGNVLSDDEVKKIAALAAPHDVPVLIDSAYAPPFPALNFTEMTPVFGENILHCLSLSKAGLPGERIGVAIGDEGIIQALESFQTNLCIHPSRYGQAIAANAINSGALAKIAEEVIRPFYQKKFAVLEDTLDTAMPKDLPWFLHRGEGAIFAWLWLQDLPITDWEFYQELKQVGVIVVPGSSFFPGLREEWPHKHQCLRISLTGSDAEIVTGMQLLAQVAERVYQRAVVSA
>CP020114.1|AVZ30084.1|1528272_1531143_-|phosphate-binding-protein-PstS
MMWQQEKKDSAIVSLALLLSLATTPMAVQLFVPTSVLAQSATEAESFPLPQTVENGTKVRIDGSMSLTAINQSFKQSFEQKYSGTNVEVAANGTDAAISALIAGKVDLIAIARALTPEEQAQGLAQVRFYEEKIAIIVNTDNPFLGILTDQQFADIFRGKITKWSELGGNESTIRFIDRPESSETRQRFRSYPSFQGAELTTGSNAIQIEADNTAEIVQQLGKDGISYAMVHHLSQLPNQRAIPINQTLPDNPNYVYSQPLYYAYRQNPSPNVASFLGFVLGSEQQTIQAAINAASGLLATPSVPQEEPETVTTPTVDPQTIAAAQQPGETTFIADEIPLWWLLLPTAVIIGLLTWVLRSRLWATPSENKAPKTLKASEENEASTLIDDTNITPPVGESLETASNDASEEVEEQKLFQTSDYSNFTELEFVDDEQSPWDIEAPASVVNIPYPQLRDVSKVTAEESQVREVVSDAQIWEIKDSSQETAEQPQVESDLIEEIISHETTDLPEDMDTEASVWDETPDNQVKTSHPTLPDEDTLNSVDLPGDMDTEANVWDETPDNQVKTSHPTLPEIPEDILNSVADAAEPIKDETVFEQPDERNIFADSHTFVGTTENEGNSESEGKVVNSTTEEPVFNLDGEQSIVLKPRNAEWAYATWYIEPSCQEALQNHGISQLGLRLYDVTDLDLSDQTPQLVQHYELESKIEEKYIAIPQSDRDYIAEIGYVTEGDRWVKIARSKKIRVFGLPLTDSTAENLPTADTTFVDLPQTNHESSIILESRTIKWAYASWYISPTDQQILQNHRSSQLYLRLYNVTDVDLSSQTPQVVQQYECDDITRDRYVSIPATDHEYIAEIGYITQANRWEMIVRSERIRVFSRPQADFWFLADTELIIHGATEPGATVNIAGKPIKIKPDGTFHLRIPFSNNSMNYIMTAIAANGEHSQTIQKTFSQENSQP
>CP020114.1|AVZ30085.1|1537610_1538711_-|precorrin-8X/cobalt-precorrin-8-methylmutase
MNAGLFTIKEFTEAVGGGITPRMVRHYHQLGLLPQPERSPSNYRLYTDRDIIRLQRIVALKNQGFQLNHIRHILEVEPQTDNLIEQLQQQYRAVMQQITQLRQTASALENLLGRDRHCQIMQAEVLSQLKHLEVETQVGIGELNQLWSGLDAEVHHHPESFTESLQRILPDLSQRSEIEQDLIAQLVLACGDVSLVSFVKISQGAIAASRESLKSGCKIVVDIPTVAAALDQTRLTHLGCQTITLIDNPHITSAPEAEAEFWEQQKWRKKLTQISENSILVIGYAPSVLLEICQAINQQKIQPALIIGLPIGFSHAPAAKRRLMQQPIPYITIEGTLGGGLLAATTLNALVETLINKPDCHCHLTC
>CP020114.1|AVZ30086.1|1538746_1540291_-|hypothetical-protein
MLLKKAITRIPRLSLQSPWLIIIIFSTLGLIGILNHSMWRDELNPWLIVRDSESFRDLIANIHYEGHPLLWYFCLAVLRRIAENPVIMQLFHLAIAISSVAIFCLYSPFNYQQKFLFSFGFFPFYEYLVISRNYAFSMLFLFAACTVYSSRKKTYVYLAILLGLLANSSAYALFISFSLLLTLLGEFCFDIEHRNQYFTHSKKFDLFLSLGITILALLLSVYIITPPADSYLHGGLNDGWVNQFDLRNLFRSLGRLFGSYLLIIPTHKRWLDLILCGLISLFIVALTVIKLSKKPFALFFYIIGNSIILAFTYLRFLGQPRHFGHLYLILIAGLWLGSYYAESTFLTNKFSLKAKTIKLGEKWQYIAFMLILYVQFLGGIGSFTKDLIIPYSASRETANYMQKNQLDNEFIVASRDANMAAISGYLGRKLYYPERQEMGSFTLFKAGRKDVEQPEILEQIASLLTSQPNNKKILLILNKKLNVNRQDLQIVSIKNFEKAWVDDERYYLYWVNKV
>CP020114.1|AVZ30087.1|1543342_1543627_-|hypothetical-protein
MRSVINKLGGFKDQVNIKETGINLNVPLAILHGNEDTVIPKQDWVTPFNQFIATNHKKMFLSFTDKHGYEPMYANHEQATIDTSFFLIFWRNRF
>CP020114.1|AVZ30088.1|1543614_1543923_-|hypothetical-protein
MGVRFFISLKAMHNIPYNLNIKSPPKPDYSNIYTSPNYQIKTYGECNQGTYVKIYIPENPYYKNESIPQAVIYLHGFALGASEIYSTHFPPQILIFRLLCVV
>CP020114.1|AVZ30089.1|1545287_1548182_-|putative-phycocyanin-operon-protein-Z
MLTTARHTNKLSLFFLFCFTLLLTLFLSLPWVSATEKPKPKPEAWQINGIVAALTDPIPEVRAKAAEHLQEYQLDNPKSQIKNYDELVKKLAEQLQDKDSAVSRSAAAEALGEMQAKEQAPQVALLLKDDDTNVRYAAAYALGEMQAKEQAPQVALLLKDDDTNVRRAAAYALGEMQAKEVVPQVALLLKDSDSDVRRAAAYALGEMQAKEVVPQVALLLKDDDTNVRRAAAEALGQMQAKEVVPQVALLLKDDDTNVRYAAAYALGQMQAKEQAPQVALLLKDSDSDVRRAAAYALGQMQAKEVVPQVALLLKDSETYVRSAAAEALGQMQAKEVVPQLALLLKDSETYVRSAAASALIKIGQQDLPVVVPVLDSVHYYPSEIGQIRFLAHFLGGGKAQVETLMQWVGKPKMPPKELEYNEGVEAMTVFQTAWKHRDSLPELQRELERQISEVAANKTIIWKAQNIKLLQNHYSNLKKVNSTHANTLQSVINNVEYRQWFFNTRITIITHAAFWLALIFAYPKFPQVQAIFFWNPWVRRIGGIGYVGFLLTWFPPFRRKLFEPFKPSLLADARLDNFNTQSYFPESRVKIPGSDDIIPITEALPSIQGQIILEGESGLGKSMFLRHLLQNSQRIVVFLPAQKCAKGVIEAIQDKLHGQAQDADFLKNLIYSGAIDICIDGLNEVTAETRAKITHFVESYFRGNIIMTTQPLEWTPPSTAKIYKLQSLEKQQIEEFLISRQPQLPQDAKVQGEDYKTACIDYLKSALNSQQAPEELDAAYRILSNPMDLTMVALMISQGKYPNLFHLQEEQYNLMAAEYLKEWNQEFPLKKFSAAVYEMRLQDKQALPADEFHQVVMSLADEKYKMVVSRQWKEKGEAKQEWYFRHDKIMDFFLVQNFIGENDAAERLLVDRMSDPRFRGVYFLLATLLPLDAAKELREALIQYAADTKDNTVSNTFVQLLRSR
>CP020114.1|AVZ30090.1|1553055_1553835_-|hypothetical-protein
MYLTWLDSNSWLLEIGNQRILLDPWLVGSLTFGNLDWFFKGSRPQERPIPENIDLILLSQGLEDHAHPETLKQLDHKIQVVASPNAAKLLPGLGYTSVTSLAHGATFNLNEQVEITAVPGSTVGYNLVENGYLLKEVSTGLTLYYEPHGSHSPEVKKFAPVDVVITPIVDVTLPLGLPIIKGRKSALEVAQWLQPQIMLPTAAGGDVIFEGLLTKFLKAEGSKEEFNSLLNQNNLTTRVIEPKPGDRLELQLQKRALKV
>CP020114.1|AVZ30091.1|1553916_1554564_-|hypothetical-protein
MHFEILIEDKSGKTALEILVPKIINTEEHTFKIHFYKGIGHIPKNLTSTSDPKKRILLDQLPRLIQGYGNTLSHFPAVLIIVCDLDDRCLSAFRNELLELVNSCHPQPKTQFCIAIEEGEAWYLGDLAAVKAAYPSAKQAVLNSYTNDEICGTWEKLADAVYSGGSQKLSKLAYEVIGKEKATWAAKISPLMDIDNNQSPSFCYFRDQLRRLSGQ
>CP020114.1|AVZ30092.1|1554568_1555861_-|hypothetical-protein
MSKVEGFRVRNYRVLRDITLGKLWNTQNAKPLTAMTAVIGKNGVGKSTIFDTFGFLADCLKNGVEEACDSRGRGGFERIRSQGQEGSIEFDIYYKEDHNARPITYELAIDIDSSGRPYVKRERLRQRRYKQTRGQPFSFLILNEGRGIAWKDQQEGKQVDEEKGDFDLSQLIEKIQRGEAEEESKETEVVELEDQRKLGIATLGSLKQHPRISGFRRFIEGWYLSYFTPDAARSLPLAGPQKHLNIHGDNLGNVVQFMEREHPKKFKSILEKISSKIPGVNKISTEKSPDGRLLLRFNDKGFEDPFYAQQMSDGTLKVFAYLLLLEDPSPPPFLCIEEPENGLYHKLLETLAMEFREHATGQQGGSQIFVTTHQPYFVNALEPDEVWVLEKGDDGFAKIKRSSEDPLINNLVSEGLPLGGLWYSDYLDAR
>CP020114.1|AVZ30093.1|1556208_1556595_-|steroid-delta-isomerase
MAQEPIETIVCAYFANIAAMNPEGWVDNFAEDAISHDPVGEPPTKVHEGYREFIGQLQAFFAKLEPTTEHIFVAGNEAAVKWTMHGLTKRGKSVVFEGITIFEINAAGKIQTTRAYWNPASMVAQLRS

Crispr_ID: CP020114_9

• CRISPR_ID: CP020114_9
• CRISPR_location: 1986659-1986771
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 1 spacers

Consensus repeat of CP020114_9

Consensus_repeat	Method
CCTCTTGTCCCCCCTGCACCCTGCACCCTGCCCCCTGC	CRISPRCasFinder

spacers of CP020114_9

>9.1|1986697|37|CP020114|CRISPRCasFinder
ACCCTGCCCCCCTGCCTCTTGTCCCCCCTGCACCCTG

CRISPR arrays and Neighbor proteins around CP020114_9

CRISPR arrays

The CRISPR arrays of CP020114_9

>merge|CP020114|9|1986659-1986771|CRISPRCasFinder
CCTCTTGTCCCCCCTGCACCCTGCACCCTGCCCCCTGCACCCTGCCCCCCTGCCTCTTGTCCCCCCTGCACCCTGCCCCCTGCCTCTTGTCCCCCCTGCACCCTGCACCCTGC

>CP020114|9|9|1986659-1986771|CRISPRCasFinder
CCTCTTGTCCCCCCTGCACCCTGCACCCTGCCCCCTGC	ACCCTGCCCCCCTGCCTCTTGTCCCCCCTGCACCCTG
CCCCCTGCCTCTTGTCCCCCCTGCACCCTGCACCCTGC

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
CP020114.1|AVZ30302.1|1986829_1988074_-|carboxy-terminal-processing-protease-CtpA	unknown	unknown	gnl|CDD|177681
CP020114.1|AVZ30305.1|1994117_1994519_-|HTH-type-transcriptional-regulator-/-antitoxin-HigA	unknown	unknown	gnl|CDD|227786
CP020114.1|AVZ30300.1|1983655_1984264_-|hypothetical-protein	unknown	unknown	unknown
CP020114.1|AVZ30311.1|2009855_2010773_-|NAD-kinase	unknown	unknown	gnl|CDD|179456
CP020114.1|AVZ30301.1|1984474_1985740_-|hypothetical-protein	unknown	unknown	gnl|CDD|100081
CP020114.1|AVZ30293.1|1957703_1958906_-|anhydro-N-acetylmuramic-acid-kinase	unknown	unknown	gnl|CDD|236579
CP020114.1|AVZ30308.1|1997943_2001810_-|sensor-histidine-kinase-TodS	unknown	unknown	gnl|CDD|223715
CP020114.1|AVZ30309.1|2008382_2008748_-|hypothetical-protein	unknown	unknown	gnl|CDD|378344
CP020114.1|AVZ30307.1|1995025_1997656_-|DNA-polymerase-III-subunit-alpha	unknown	unknown	gnl|CDD|181933
CP020114.1|AVZ30297.1|1971640_1972669_-|hypothetical-protein	unknown	unknown	gnl|CDD|379252
CP020114.1|AVZ30303.1|1990087_1990270_-|hypothetical-protein	unknown	unknown	unknown
CP020114.1|AVZ30298.1|1978889_1980473_-|hypothetical-protein	unknown	unknown	gnl|CDD|366375
CP020114.1|AVZ30292.1|1957106_1957601_-|hypothetical-protein	unknown	unknown	unknown
CP020114.1|AVZ30295.1|1963154_1963487_-|hypothetical-protein	unknown	unknown	gnl|CDD|367958
CP020114.1|AVZ30306.1|1994502_1994802_-|mRNA-interferase-HigB	unknown	unknown	gnl|CDD|378301
CP020114.1|AVZ30294.1|1962231_1963152_-|pantothenate-kinase	unknown	unknown	gnl|CDD|226691
CP020114.1|AVZ30304.1|1992907_1993768_-|thiosulfate/3-mercaptopyruvate-sulfurtransferase	unknown	unknown	gnl|CDD|225450
CP020114.1|AVZ30296.1|1966604_1970216_-|signal-transduction-histidine-protein-kinase-BarA	unknown	unknown	gnl|CDD|223715
CP020114.1|AVZ30299.1|1980564_1983024_-|serine/threonine-protein-kinase-F	unknown	unknown	gnl|CDD|270916
CP020114.1|AVZ30310.1|2008787_2009498_-|response-regulator-MprA	unknown	unknown	gnl|CDD|223816

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
CP020114.1|AVZ30302.1|1986829_1988074_-|carboxy-terminal-processing-protease-CtpA	gnl|CDD|177681	PLN00049, PLN00049, carboxyl-terminal processing protease; Provisional.	1.26912e-140
CP020114.1|AVZ30305.1|1994117_1994519_-|HTH-type-transcriptional-regulator-/-antitoxin-HigA	gnl|CDD|227786	COG5499, COG5499, Predicted transcription regulator containing HTH domain [Transcription].	1.1387e-06
CP020114.1|AVZ30293.1|1957703_1958906_-|anhydro-N-acetylmuramic-acid-kinase	gnl|CDD|236579	PRK09585, anmK, anhydro-N-acetylmuramic acid kinase; Reviewed.	1.23987e-177
CP020114.1|AVZ30311.1|2009855_2010773_-|NAD-kinase	gnl|CDD|179456	PRK02649, ppnK, NAD(+) kinase.	0
CP020114.1|AVZ30299.1|1980564_1983024_-|serine/threonine-protein-kinase-F	gnl|CDD|270916	cd14014, STKc_PknB_like, Catalytic domain of bacterial Serine/Threonine kinases, PknB and similar proteins. STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. This subfamily includes many bacterial eukaryotic-type STKs including Staphylococcus aureus PknB (also called PrkC or Stk1), Bacillus subtilis PrkC, and Mycobacterium tuberculosis Pkn proteins (PknB, PknD, PknE, PknF, PknL, and PknH), among others. S. aureus PknB is the only eukaryotic-type STK present in this species, although many microorganisms encode for several such proteins. It is important for the survival and pathogenesis of S. aureus as it is involved in the regulation of purine and pyrimidine biosynthesis, cell wall metabolism, autolysis, virulence, and antibiotic resistance. M. tuberculosis PknB is essential for growth and it acts on diverse substrates including proteins involved in peptidoglycan synthesis, cell division, transcription, stress responses, and metabolic regulation. B. subtilis PrkC is located at the inner membrane of endospores and functions to trigger spore germination. Bacterial STKs in this subfamily show varied domain architectures. The well-characterized members such as S. aureus and M. tuberculosis PknB, and B. subtilis PrkC, contain an N-terminal cytosolic kinase domain, a transmembrane (TM) segment, and mutliple C-terminal extracellular PASTA domains. The PknB subfamily is part of a larger superfamily that includes the catalytic domains of other protein STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase.	1.81354e-52
CP020114.1|AVZ30301.1|1984474_1985740_-|hypothetical-protein	gnl|CDD|100081	cd06160, S2P-M50_like_2, Uncharacterized homologs of Site-2 protease (S2P), zinc metalloproteases (MEROPS family M50) which cleave transmembrane domains of substrate proteins, regulating intramembrane proteolysis (RIP) of diverse signal transduction mechanisms. Members of the S2P/M50 family of RIP proteases use proteolytic activity within the membrane to transfer information across membranes to integrate gene expression with physiologic stresses occurring in another cellular compartment. In eukaryotic cells they regulate such processes as sterol and lipid metabolism, and endoplasmic reticulum stress responses. In prokaryotes they regulate such processes as sporulation, cell division, stress response, and cell differentiation. This group includes bacterial, eukaryotic, and Archaeal S2P/M50s homologs with additional putative N- and C-terminal transmembrane spanning regions, relative to the core protein, and no PDZ domains.	2.17083e-66
CP020114.1|AVZ30309.1|2008382_2008748_-|hypothetical-protein	gnl|CDD|378344	pfam09996, DUF2237, Uncharacterized protein conserved in bacteria (DUF2237). This domain, found in various hypothetical bacterial proteins, has no known function.	1.30391e-78
CP020114.1|AVZ30307.1|1995025_1997656_-|DNA-polymerase-III-subunit-alpha	gnl|CDD|181933	PRK09532, PRK09532, DNA polymerase III subunit alpha; Reviewed.	0
CP020114.1|AVZ30297.1|1971640_1972669_-|hypothetical-protein	gnl|CDD|379252	pfam13546, DDE_5, DDE superfamily endonuclease. This family of proteins are related to pfam00665 and are probably endonucleases of the DDE superfamily. Transposase proteins are necessary for efficient DNA transposition. This domain is a member of the DDE superfamily, which contain three carboxylate residues that are believed to be responsible for coordinating metal ions needed for catalysis. The catalytic activity of this enzyme involves DNA cleavage at a specific site followed by a strand transfer reaction.	0.000432813
CP020114.1|AVZ30298.1|1978889_1980473_-|hypothetical-protein	gnl|CDD|366375	pfam00931, NB-ARC, NB-ARC domain.	5.36992e-08
CP020114.1|AVZ30308.1|1997943_2001810_-|sensor-histidine-kinase-TodS	gnl|CDD|223715	COG0642, BaeS, Signal transduction histidine kinase [Signal transduction mechanisms].	2.03906e-53
CP020114.1|AVZ30295.1|1963154_1963487_-|hypothetical-protein	gnl|CDD|367958	pfam04483, DUF565, Protein of unknown function (DUF565). Predicted transmembrane protein found in plants, chloroplasts and cyanobacteria. This family is also known as YCF20.	7.70056e-19
CP020114.1|AVZ30306.1|1994502_1994802_-|mRNA-interferase-HigB	gnl|CDD|378301	pfam09907, HigB_toxin, HigB_toxin, RelE-like toxic component of a toxin-antitoxin system. HigB_toxin is a family of RelE-like prokaryotic proteins that function as mRNA interferases. HigB cleaves translated mRNA only, and cleavage depended on translation of the target RNAs. HigB belongs to the RelE super-family of RNases. The toxin-antitoxin gene-pair is induced by environmental stress factors.	1.73274e-33
CP020114.1|AVZ30294.1|1962231_1963152_-|pantothenate-kinase	gnl|CDD|226691	COG4240, COG4240, Predicted kinase [General function prediction only].	8.12229e-112
CP020114.1|AVZ30304.1|1992907_1993768_-|thiosulfate/3-mercaptopyruvate-sulfurtransferase	gnl|CDD|225450	COG2897, SseA, Rhodanese-related sulfurtransferase [Inorganic ion transport and metabolism].	8.01925e-73
CP020114.1|AVZ30296.1|1966604_1970216_-|signal-transduction-histidine-protein-kinase-BarA	gnl|CDD|223715	COG0642, BaeS, Signal transduction histidine kinase [Signal transduction mechanisms].	2.97441e-47
CP020114.1|AVZ30310.1|2008787_2009498_-|response-regulator-MprA	gnl|CDD|223816	COG0745, OmpR, Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain [Signal transduction mechanisms / Transcription].	1.93224e-78

Protein sequences

>CP020114.1|AVZ30301.1|1984474_1985740_-|hypothetical-protein
MSNWLLFQWGRRTLRDTQTQPQTIESQSAIHPTAESVPVRPIEPTEETQLRNCFPWSVYYIHNIEYRPQAVICRGQIRTTPTQAYQQIKANIEAEFGDRFLLIFQEGFNGKPFFVLVPNTQATRNTSQPDQITRPGLALFLVIATLVTTTLVGATIAGADAKQLASDPSILWQGLPYALGLMTILGIHELGHYLTARYYKIRATLPYFIPLPVFLGTFGAFIQMRSPIPHRKALFDVSIAGPLAGFVITLPLLLWGLVNSEVVTMTNQTGLLNPEALNPKSSILLALLSKLALGSQLTATSAINLHPIAIAGFLGLIVTALNLMPVGQLDGGHIVHAMFGQKTAIVIGQISRLLLLLLSLVQQGFLLWAIILLFIPLIDEPALNDVTELDNKRDILGLMAMALLIIIVLPLPQAIANLLQI
>CP020114.1|AVZ30300.1|1983655_1984264_-|hypothetical-protein
MLFTNPLTIAATSFVLVLGTQNPAVALVNPSISAIESVSSTTKVRTPKQPILIAENDTYWGPNRDFKILMPGKTVTNTADQLISVSATESVYMVIHRNFPQASRIPIPQLRQVLQSAMRESIGLDGKIIQTTNLVINQHPGLELLMQHSDGTLGKYRAFLVNQRFYLMGTLTKNELTTESVNFFDSFRVYPENITYSNWDVY
>CP020114.1|AVZ30299.1|1980564_1983024_-|serine/threonine-protein-kinase-F
MPLILQGRFLPKRLLVSEQEQGGIGITFLGEDLDFEKELRVIKQLHPRQTLSSTLMQRVEESFKREAKILSKLNYQQIPRAWAFFVVEADSSKYPPKFFYLVQDYIEGENLSQILKKQRKFSEAEVLDILKEILKILTYIHNYDHKLKTIHRDIKPANIMRRQSDGQLYLIDFGAVKQVTEGLPGLSAETTSMMFTPAFAPPEQHDQAEVSPASDLYALATTCVSLLTGCKNPNQLLFNSNWKEHANVSNHNFANVIDSMLKYEKGDRPQSAQDVLDALAEKQPNPLQPLLDWLKKFPQQRRWIILAGLALLGVAIAIILNFIETTSSEPHLSQYFSRGENALINENPATSRIPQCTDAYELKLKGIDAFREASSLDSVAGFRQAENYFKEATDKFREGAVQTADSENKCQVDPETLIYYYNSKAAQNARDLPTIAVVIPSVPEFRGTALEILRGVAQVQSIQSTADSPVFQIIIVKDNSNNPEKFTEIVKKLSNQNNNIPGELEYFKDSQILGVIGHSTSDDTWKAGAIYQEQQLVLISPTSTAVRKPNPDNQYVFRTAADDYIAATNLAKYYLNNQPQQGKILILFESESQYSESLKEEFARTLLRLSPNRRPTLIQEYCDLSTQTNSKDCIKKATAANVQALMLAPSRNHLETALEITKKAKDNAKSENRDLQLLAGDVLYGKETFEKLGNLANGMVVAVSSHADIANSDFTQKAIELWSTAKVSWRTLTSYDAAQSFFQALTELSPDGRINPTREEVYQKLKTISAPGATQVSVEFDTYGDRKEAIGVGILAQASSSDGSSDEYRFTYLETPERK
>CP020114.1|AVZ30298.1|1978889_1980473_-|hypothetical-protein
MQTLKASESGKILIKESRMRKIEQARTKRIGLQGIIERAWDMDEIFLREASKILEPDQNWDNSQTFAVALATWKRFREAKQPIRADTFKAFCQVLDLEWENVVENDVESRRNISEAPDLSSFSGRTAELAELQQWLIKNGCRLVVVHGMGGIGKSALARRLVNQIADKYDYIIWLSLASSPPFSEVLLKLGQFLSKGEKEPDDITQIMQYLHHQRCLIVLDGWEEITGNQSTDYINYSTFVERVATEAHRSSLILLSRKVTHDIAILKGQLVRFKKLGGLEYEEARQIFRSEGISGTDIELEKLSKDYSNPWMIKVIAQQVKTVFPDYLPPFIIDRSVLVSDPIKKFLDNQFKRLSNIEINLIYWVAIRRNSASWNQLLQDNQELLSDKQLFETLHNLIAGHSFIDKNIEDIPTLYILDPVILKYTTERFVEQNYQEIIQLFNSESIQGNELFITHSFVTEHPQDEELNQQQMRRIVKPIQKMLLAKLCRQQQLHDELKNVTSLFKNQGLSPGYAYQNISHLISACY
>CP020114.1|AVZ30297.1|1971640_1972669_-|hypothetical-protein
MLVISMRFTKLNYCQYLLSSQINYTITNLAEHLEHISHDAINYYLKREKLTPRLLWENVKDVIELDANSYIIFDDSILDKRYSKEIEMVRKQYSGNEHGIIKGIGVVSCVYVNPKVQRFWVIDYRIFNPDVDGKTKIDHVKDMLQNLVYQKLLPFDTVLMDTWYAVHSLMLYIDSLEKVYYCPLKNNRLVDDTLGKEKYKRIDLLQWSEEELECGKIIKIKGFPAQKKVKLFRVAVSTNRTDYVATNDLSQSSTNVVQKVCKIRWKIEEFHREIKQLTGIESCQCRKARLQRNHIACAMLVWIRLKNLAYQTGQTVYQIKHNLLSNYLIQQLKRPSISMCLV
>CP020114.1|AVZ30296.1|1966604_1970216_-|signal-transduction-histidine-protein-kinase-BarA
MSMLQYPLYDFLATVPNCVETSTLAIVLEIFEQEQCDRLVVVNQQQCPLGLVQSARLTQKLLAASGDNQFLDLQQPLSTWDKAIIEPLKTLPASYCVEQFKLLLRYQPSQTNSNFDWALIDGSGQFLGLLNSARLLKALAKEKLAMNGERTGYSPSTQRTSENISPDHAHTSSTRAKTHGRDDHKSGLAQSAYSSAEYKSRARRSRSPRINQRQPSQKSLVQLLDLLPWPLMLQTQTGEVVAKNPAWWQQLGAFKDPEGIRQQVEAILAPTQTKTATEYASQQATKFYPDHKGNAHPGQGQSIPRQSHSGEHTFVSPPEAPPLAPSSPPPSHNRSDFGVSAQLNAEANGKSLHQQSTATTTTSNRCFLDSPMGTCTCVVEVQNGQERIWQFAKIRLDSPKSKLLFREMFAGEFTFEQTAATLRTQRGHDLCLILATDVTEQHQIYKELAAKNADLIQLNRLKDEFLACISHELKTPLTAVLGLSRLLVDQQLGELNERQARYAGLIHQSGRHLMSVVNDILDLTRMETGQMDLALVPVTIRTVCDRALTEAKAIHTQTTKASATPTPTANSEMEFSISIEPGLEQIVADELRLRQMLVHLLSNAFKFTQNSGEIGLRVSRWEGWIAFTVWDTGIGIPEHQQHLIFQKFQQLENPLTRQFEGTGLGLVLTRALARLHGGDVSFLSREGKGSQFTLLLPPSPPTTGFTEPEMGIREDHKIQFPETREVPASARGRMTTTPQHQPTSSQRLVLVVEAVARYIEDLTDQLKGLGYRVVIARSGTEAVEKARRLQPKAIFLNPLLPLLSGWDVLTLLKSDAVTKHIPVMVTATGAEKEQAYANRADGFLNLPVQHQALVPLLENLVAQPAIGQSGLGNHKIAPTTTPLRILRLVEQNLASINPHPSLREHRVIEVDDLDQAELLARVWQFDVILLDAEGSLAQTYLEQLTQHPILADIPLVTCDVATTLAASQIPGLSVFPYLTAFSRDQDNHSDPTDPLLSVLQIASGICCPPSILVVDLTMLDDLPQIRRKHLQNGQTVKKSSVNNDTTERGSEWFQALIQYLQTAGLKAAMGRCWAEVLQQMRHQSVDLLLISLGDSSVHQEVITKLKALADLPFELPPILIIDQRFHHTFTHSLFEGSPGESNQPSQSKLDAAENLMSELAQLVVRINSRKQDIAAQILPRSISMEDLLTQINQALAGLTQKLS
>CP020114.1|AVZ30295.1|1963154_1963487_-|hypothetical-protein
MQNTRLTNLFDSIARRLGQWFLNPWRRLSLLLINFFFGFFLGTAISTIAGQRGLLDIVIAGILVVLTEVTSRIFYSPSFLSKRSLLVESLNILKVGFIYSMFVESFKLGS
>CP020114.1|AVZ30294.1|1962231_1963152_-|pantothenate-kinase
MDAWASDVDTTTVGLLNSILPSFSEFCHTHLHKPPAEMLAVLCDLWLPLSIKLATHRQALGRPLIQGILGGQGTGKTTMCQVLSLILQQLGYRTLSLSLDDLYKSYSERLILTQQDPRLIWRGPPGTHDLDLGLKVLEQIRQGESPVRVPRFDKSAYHGAGDRTTPEIVENIDIVLFEGWFVGVRPIDPDVFNHAPPPIVTEEDRVFARDMNQRLSDYLPLWERLDSLMVLYPTDYRCSMTWRKQAEQQMIAAGKLGMSDAQIEEFVNYFWRSLHPELFITPLVKSPTAVDLVIAINPDHSVSAVK
>CP020114.1|AVZ30293.1|1957703_1958906_-|anhydro-N-acetylmuramic-acid-kinase
MPPTKKVTVPTRVIGLISGTSVDGIDAALVEISGSDLDLKVSLLAGETYPYTPELRAKILAVGAGVAISMAELAEIDDAIALAFAQAAQNIQIGHQPATLIGSHGQTVYHRPPQGVRQKSNCLGYSLQLGRGALIAHVTGITTVSNFRLADINVGGHGAPLVPRVDAFLLSDSAEGRCIQNIGGIGNVAYIPPRRGYWLSKIRAWDTGPGNSLLDLAVQHLTDGAKSYDENGNWAASGIPCDSLVAQWLQEDYFHLPPPKSTGRELFGVAYLHQCLKDAEPYQLNPADLLATLTELTVASIAHSYQSFLPEMPQQVLLCGGGSRNLYLKQRLQLLLPSVPVLTTDEVGLNADFKEAIAFAVLAYWRQLGLPGNLPAATGAPCEILLGEIHSQLGISRVDI
>CP020114.1|AVZ30292.1|1957106_1957601_-|hypothetical-protein
MSCCTWVIGSRQGTYKVAHTFLLEPGRWTMEGNWLERHGMPISVKGMTLVAWSRDNWFTMATKLIFPSSDRSEISLQYKGRLDNGERQYSFLLQHNVLGKIEGEGWIGVDTIMQHYWVIGDRQRRSGFETIHRISEQTYYLSSTFMAGHVLTNTMEASLQRQSA
>CP020114.1|AVZ30302.1|1986829_1988074_-|carboxy-terminal-processing-protease-CtpA
MQKQVFRLGFSLLVAFWLALGALVQPAAALTDEQKLVSEVWRIVNRTYLDETFNHQNWSQARQKALEKPLKNNEAAYAAIQKMLKTLGDPFTRFLDPQQYRSLQVSTSGELTGVGLQIALNPETGKLEVVTPILDSPADAAGIKPRDRILKIEGILTENLTLDEAATRMRGPVGSSVTLLIERDGIGEKEVRIVRDRIELNPVVADLRFSPQGTPIGYLRLTQFNANASMELAHAISSLEKKGAAAYILDLRNNPGGLLQSGIEIARLWLNSGTIVYTVNRQGIQGSFESFGPALTDDPLVILVNEGTASASEILAGALQDNGRATLVGETTFGKGLIQSLFELSDGSGLAVTIAKYETPQHRDINKLGIKPDTVISQESITRAQIATEADLQYQAAVELLKTNSVLAGVGSGE
>CP020114.1|AVZ30303.1|1990087_1990270_-|hypothetical-protein
MSVFVAFWQLSRWEYIFSPQLKTPDTTWITKIDTCRFQNYIGQYEQLSKNTSVVIAWCSF
>CP020114.1|AVZ30304.1|1992907_1993768_-|thiosulfate/3-mercaptopyruvate-sulfurtransferase
MDTKLLIYPQELTSLLAEDSLQIAIIDTRSSEEYIKSHLPGAINIRELFTYLLENSQADGLKKLSQHFSELLSKAGISGAEKLIVYEDALNQGYGQSCRAAFLLKYLGCTQVSVLHGGYKAWLTAGLPTTQGEVPIRKSKVFTAHPDAAIMVTTEEMLQALDDPKIIKLDVRDRNEWQGLSSSPYSPDFCPRKGRIPNAVWLEWHRLMNCDAEIPMFRSPAEILEICQSVGITSHAKVYIYCFKGSRAANTMIALQQAGIYSRNYFGSWNEWSRDFSLPIDHRIII
>CP020114.1|AVZ30305.1|1994117_1994519_-|HTH-type-transcriptional-regulator-/-antitoxin-HigA
MTLTFNLDKYKELLTAYLPKLIKTEAENEQALAIVEDLMHRERTPEENEVYQLLITLIEKFEQEYYQPSQQKNPRDMLLFILEESDKNKEDLVAVLGSEDIFNNIVNGQEKINTEQSRKLGYFFHVDSSLFME
>CP020114.1|AVZ30306.1|1994502_1994802_-|mRNA-interferase-HigB
MHLISFRILREYAQNHADCQEALNNWYKVAIKARWSKLVEVQQVFPKAEAVSNFTVFNIKGNKYRLIVSIDYEGQLIYIKYILTHAEYDKENWKDDPYF
>CP020114.1|AVZ30307.1|1995025_1997656_-|DNA-polymerase-III-subunit-alpha
MSFVPLHIHSDYSLLDGASQLPELVDHAISLGMKAIALTDHGVMYGAVELIKICRSKSIKPIIGNEMYIINGDFTKQERRPKYHQVVLAKNTQGYKNLVKITTISHLDGVQGKGIFSRPCINKDLLKQYREGLIVTSACLGGEIPQAILSNRPDAARKIAKWYKEIFGDDYYLEIQDHGSQEDRIVNVEIVKIARELNIKFIATNDSHFISCFDVEAHDALLCIQTGQLISEDKRMRYSGTEYLKSADEMKQLFRDHLPDDIIAEAIATTEEVADKVGVYDIMGDPQIPTPPIPSGHTPDTYAEEVAWNGLLERLNRKSRSEVDPVYKERLEYELKMLQTMGFSTYFLVVWDYIKFARDNNIPVGPGRGSAAGSLVAYAMKITNIDPVHHGLLFERFLNPERKSMPDIDTDFCIEKRDKVIEYVTEKYGKDRVAQIITFNRLTSKAVLKDVARVLNIPYGEADKMAKLIPVVRGKPTKLKVMISDKTPAPEFKERYDNNAHVRHWLDMAIRIEGTNKNFGVHAAGVVISADPLDEIVPLQRNNDGSVITQYFMEDLESMGLLKMDFLGLRNLTLIQRTIDLIEETKGYRIDPDEITGQERKAQKILAKGEHSNLPKDVKKSYELLEAGELEGIFQLESSGMRQIVRDLKPSNIEDISSILALYRPGPLDAGLIPKFINRKHGREAIDYQSAILEPILNETYGIMVYQEQIMKIAQDMAGYSLGQADLLRRAMGKKKVSEMQKQREKFVDGAAKNGVKKQVADELFEQMLKFAEYCLSYDTEILTVEYGYIPIGEIVEKAIECSVYSVDNNGNVYTQPIAQWHNRGEQEVFEYSLEDGSTIRATKDHKFMTTDGQMLPIDEIFAQELDLLQVHGLPK
>CP020114.1|AVZ30308.1|1997943_2001810_-|sensor-histidine-kinase-TodS
MFKINPLQLEYLHRLRVVQAASAIAILVGVLVLLGWFFDLELLKRSGNNLVTMKPNAALGFLLSGLSLWLLQMAQGKKDNSSIAYLRGSRICAAIVTLIGLLTLSQYLFGWNLGIDELLFTDKPDAIFTSHPGRMGFNTALNFILVGIALEILAHPKNNRSCWYVQILALIAALISLQVVISYAYKVQIFSRIASFTTSMALHTGLTFIVLCVGILWARPDKGLMQVVMSDTYGGLIARRLLLAAIAIPLVLGWLILQGQLAGKYGSAFALLLFAIILIIIFTIFIWQSSTVITRLCRQHDEAQKKLRAYEEKLRSFVDANVIGIKFGDVYGGIHEANDAFLQMIGYTREDLLTGKLNWRNITPPEHVYSDKQGIAEAQGNPNGACTPYEKEYIHKDGRRIPVLVGYVLVGENREESVVFILDLSERKQAKQQIVQLNKDLQRRIAELQTLFQVIPIGIGIAEDAECKTIRANPCLAQQLGVSSDENVSLSAPSAERPTSFKVYREGRELLPEELPMQYSAAHGVEMLNCELDIVHENGKVVKLLEHVAPLFDEEGDSRGSVGAFLDITERKQVEETIQNQQKWLEDVLNMMPLPLLFIEPETARVTFANRAADQLAGGEFPKDVPVEEYGNFYHCTDVEGNRIPNEQMPGVRLARGERLDGLEVDWYTSGSVRSLLLFADTLPAMHGHPATCVSAFQDITNLKEIEKARTLNYKRLQLLFNTASDLLSSQQPLALIHSFYQKLAEQIGLDVYFNYLAEDNSQVMHLVSFTGVSEEIAKEYECLKFGQSVCGIVAQERHPIYIENVQQSTDPKTELIRSLGITAYYGYPLIASARLLGTLCFGSRSCSRFTENQRGMMQAVCDQIAIAMERSSLIASLQQQTEQLREANRMKDEFLAILSHELRSPLNAILGWAQLLRSSRQLSDIQIAKAMETIERNARMQTQLVEDLLDISRMIRGKLHLHVCTCNLVPLIESAVETVHLAAQAKEIDLRISLIPSPEMPQDHSRFLVSGDIERLQQIIWNLLSNAIKFTPIGGMIEIRLSQSSEHGEKLARTITSYAQIQVIDTGIGIKPEFLPYVFDRFRQADSSSTRSYGGLGLGLAIVRHLVELHGGIISVDSQGENEGATFTVKLPLLLESQAVIPDSVNQNNADFAYPSPVPSLLGVRVLVVDDEMDTRDFISNVLEECQAEVKAVKSVSEALQVIIDWKPDVLVSDIAMPEEDGYSLIRKVRSQPQEQGGKIPAAALTAYARAEDRTRAIQEGYQLHLPKPIEPAELATVVASLVIRNS
>CP020114.1|AVZ30309.1|2008382_2008748_-|hypothetical-protein
MADAKNVLGTDLESCCTSPMTGYYRDGFCTTGGQDFGMHVVCAQVTAEFLEYTKLQGNDLSTPVPQFNFPGLRPGDRWCLCAARWQEALEAGVAPPVVLSATHARALEACSLEDLKKHAVR
>CP020114.1|AVZ30310.1|2008787_2009498_-|response-regulator-MprA
MITIAHSPCVLVIETDESLANQLSFDLREAGYEAILAHNAASGLQYCRDADGGQSHRQPALIVVDRMLSGESGLSLCKNLRSAGMCAPVLVLMARDTVDDRVACLEAGADDYFLKPYRPEDFLKLIRLYLKPEVDTSEQLRFGDLILDVATRRAIHNGRTIDLTMKEFELLKFLMEHPREVLTREQILENVWGYDFMGESNVIEVYIRYLRLKIEDESHKRLIQTVRGVGYVLRES
>CP020114.1|AVZ30311.1|2009855_2010773_-|NAD-kinase
MPKAGIIYNDVKPIAARIAIELKDKLTAAGWDVFVTTSIGGILGYSKPESPVCHTPIEGLTPPGFDSEMKFAVVLGGDGTVLAASRLVAPAGIPLLTVNTGHMGFLTETYVNQLPQAIEMAMAGDFEIEEQAMLTVKVFRGESVLWEALCLNEMVLHREPLTSMCHFEIVVGRHAPVDIAADGVIVSTPTGSTAYSLSAGGPVITPGVPVLQLVPICPHSLASRALVFPDTETVNIYAVNIPRLVMIVDGNGGCYIFPEDRVYLERSPYKVRFIRLQRREFFRILREKLGWGLPHIAKPSSVELP

Crispr_ID: CP020114_10

• CRISPR_ID: CP020114_10
• CRISPR_location: 2515639-2515717
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 1 spacers

Consensus repeat of CP020114_10

Consensus_repeat	Method
AAATCATAGTCAAATCATAGTCAAAAA	CRISPRCasFinder

spacers of CP020114_10

>10.1|2515666|25|CP020114|CRISPRCasFinder
GGCAAGGATGATTGTTTAGGTTTTG

CRISPR arrays and Neighbor proteins around CP020114_10

CRISPR arrays

The CRISPR arrays of CP020114_10

>merge|CP020114|10|2515639-2515717|CRISPRCasFinder
AAATCATAGTCAAATCATAGTCAAAAAGGCAAGGATGATTGTTTAGGTTTTGACCTCATAGTCAAATCATAGTCAAAAT

>CP020114|10|10|2515639-2515717|CRISPRCasFinder
AAATCATAGTCAAATCATAGTCAAAAA	GGCAAGGATGATTGTTTAGGTTTTG
ACCTCATAGTCAAATCATAGTCAAAAT

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
CP020114.1|AVZ30533.1|2514438_2515605_-|tyrosine-recombinase-XerC	unknown	unknown	gnl|CDD|271189
CP020114.1|AVZ30531.1|2512309_2513161_-|hypothetical-protein	unknown	unknown	unknown
CP020114.1|AVZ30541.1|2523711_2525067_-|Xaa-Pro-aminopeptidase	unknown	unknown	gnl|CDD|238520
CP020114.1|AVZ30526.1|2505716_2506424_-|ribosomal-RNA-small-subunit-methyltransferase-E	unknown	unknown	gnl|CDD|367950
CP020114.1|AVZ30530.1|2511293_2512292_-|hypothetical-protein	unknown	unknown	gnl|CDD|259851
CP020114.1|AVZ30543.1|2539229_2540651_-|isocitrate-dehydrogenase	unknown	unknown	gnl|CDD|180944
CP020114.1|AVZ30539.1|2521068_2521329_-|30S-ribosomal-protein-S16	unknown	unknown	gnl|CDD|176950
CP020114.1|AVZ30527.1|2506525_2508445_-|sulfite-reductase	unknown	unknown	gnl|CDD|131097
CP020114.1|AVZ30535.1|2518070_2518859_-|hypothetical-protein	unknown	unknown	unknown
CP020114.1|AVZ30537.1|2519560_2520514_-|PhoH-like-protein	unknown	unknown	gnl|CDD|224616
CP020114.1|AVZ30538.1|2520647_2521106_-|hypothetical-protein	unknown	unknown	gnl|CDD|224750
CP020114.1|AVZ30532.1|2513299_2513959_-|hypothetical-protein	unknown	unknown	unknown
CP020114.1|AVZ30536.1|2518864_2519272_-|hypothetical-protein	unknown	unknown	gnl|CDD|377631
CP020114.1|AVZ30529.1|2510961_2511288_-|divalent-cation-tolerance-protein-CutA	unknown	unknown	gnl|CDD|376987
CP020114.1|AVZ30525.1|2504505_2505576_-|hypothetical-protein	unknown	unknown	gnl|CDD|276809
CP020114.1|AVZ30528.1|2508663_2509200_-|hypothetical-protein	unknown	unknown	unknown
CP020114.1|AVZ30534.1|2516162_2517983_-|lipid-A-export-ATP-binding/permease-protein-MsbA	unknown	unknown	gnl|CDD|224055
CP020114.1|AVZ30524.1|2502920_2503589_-|extracellular-esterase-EstB	unknown	unknown	gnl|CDD|224001
CP020114.1|AVZ30542.1|2526508_2527123_-|HTH-type-transcriptional-repressor-KstR2	unknown	unknown	gnl|CDD|224228
CP020114.1|AVZ30540.1|2521446_2522919_-|signal-recognition-particle-protein	unknown	unknown	gnl|CDD|236780

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
CP020114.1|AVZ30533.1|2514438_2515605_-|tyrosine-recombinase-XerC	gnl|CDD|271189	cd01189, INT_ICEBs1_C_like, C-terminal catalytic domain of integrases from bacterial phages and conjugate transposons. This family of tyrosine based site-specific integrases is has origins in bacterial phages and conjugate transposons. One member is the integrase from Bacillus subtilis conjugative transposon ICEBs1. ICEBs1 can be excised and transfered to various recipients in response to DNA damage or high concentrations of potential mating partners. The family belongs to the superfamily of DNA breaking-rejoining enzymes, which share the same fold in their catalytic domain and the overall reaction mechanism. The catalytic domain contains six conserved active site residues. Their overall reaction mechanism involves cleavage of a single strand of a DNA duplex by nucleophilic attack of a conserved tyrosine to give a 3' phosphotyrosyl protein-DNA adduct. In the second rejoining step, a terminal 5' hydroxyl attacks the covalent adduct to release the enzyme and generate duplex DNA.	1.23955e-13
CP020114.1|AVZ30541.1|2523711_2525067_-|Xaa-Pro-aminopeptidase	gnl|CDD|238520	cd01087, Prolidase, Prolidase. E.C. 3.4.13.9. Also known as Xaa-Pro dipeptidase, X-Pro dipeptidase, proline dipeptidase., imidodipeptidase, peptidase D, gamma-peptidase. Catalyses hydrolysis of Xaa-Pro dipeptides; also acts on aminoacyl-hydroxyproline analogs. No action on Pro-Pro.	1.44352e-101
CP020114.1|AVZ30526.1|2505716_2506424_-|ribosomal-RNA-small-subunit-methyltransferase-E	gnl|CDD|367950	pfam04452, Methyltrans_RNA, RNA methyltransferase. RNA methyltransferases modify nucleotides during ribosomal RNA maturation in a site-specific manner. The Escherichia coli member is specific for U1498 methylation.	4.5228e-70
CP020114.1|AVZ30527.1|2506525_2508445_-|sulfite-reductase	gnl|CDD|131097	TIGR02042, Sulfite_reductase, ferredoxin-sulfite reductase. Distantly related to the iron-sulfur hemoprotein of sulfite reductase (NADPH) found in Proteobacteria and Eubacteria, sulfite reductase (ferredoxin) is a cyanobacterial and plant monomeric enzyme that also catalyzes the reduction of sulfite to sulfide. [Central intermediary metabolism, Sulfur metabolism].	0
CP020114.1|AVZ30543.1|2539229_2540651_-|isocitrate-dehydrogenase	gnl|CDD|180944	PRK07362, PRK07362, NADP-dependent isocitrate dehydrogenase.	0
CP020114.1|AVZ30539.1|2521068_2521329_-|30S-ribosomal-protein-S16	gnl|CDD|176950	CHL00005, rps16, ribosomal protein S16.	6.15736e-39
CP020114.1|AVZ30530.1|2511293_2512292_-|hypothetical-protein	gnl|CDD|259851	cd00569, HTH_Hin_like, Helix-turn-helix domain of Hin and related proteins. This domain model summarizes a family of DNA-binding domains unique to bacteria and represented by the Hin protein of Salmonella. The basic HTH domain is a simple fold comprised of three core helices that form a right-handed helical bundle. The principal DNA-protein interface is formed by the third helix, the recognition helix, inserting itself into the major groove of the DNA. A diverse array of HTH domains participate in a variety of functions that depend on their DNA-binding properties. HTH_Hin represents one of the simplest versions of the HTH domains; the characterization of homologous relationships between various sequence-diverse HTH domain families remains difficult. The Hin recombinase induces the site-specific inversion of a chromosomal DNA segment containing a promoter, which controls the alternate expression of two genes by reversibly switching orientation. The Hin recombinase consists of a single polypeptide chain containing a C-terminal DNA-binding domain (HTH_Hin) and a catalytic domain.	0.00345195
CP020114.1|AVZ30542.1|2526508_2527123_-|HTH-type-transcriptional-repressor-KstR2	gnl|CDD|224228	COG1309, AcrR, Transcriptional regulator [Transcription].	1.11059e-17
CP020114.1|AVZ30537.1|2519560_2520514_-|PhoH-like-protein	gnl|CDD|224616	COG1702, PhoH, Phosphate starvation-inducible protein PhoH, predicted ATPase [Signal transduction mechanisms].	1.98859e-145
CP020114.1|AVZ30538.1|2520647_2521106_-|hypothetical-protein	gnl|CDD|224750	COG1837, COG1837, Predicted RNA-binding protein (contains KH domain) [General function prediction only].	5.20071e-21
CP020114.1|AVZ30524.1|2502920_2503589_-|extracellular-esterase-EstB	gnl|CDD|224001	COG1075, LipA, Predicted acetyltransferases and hydrolases with the alpha/beta hydrolase fold [General function prediction only].	6.74749e-35
CP020114.1|AVZ30540.1|2521446_2522919_-|signal-recognition-particle-protein	gnl|CDD|236780	PRK10867, PRK10867, signal recognition particle protein; Provisional.	0
CP020114.1|AVZ30529.1|2510961_2511288_-|divalent-cation-tolerance-protein-CutA	gnl|CDD|376987	pfam03091, CutA1, CutA1 divalent ion tolerance protein. Several gene loci with a possible involvement in cellular tolerance to copper have been identified. One such locus in eubacteria and archaebacteria, cutA, is thought to be involved in cellular tolerance to a wide variety of divalent cations other than copper. The cutA locus consists of two operons, of one and two genes. The CutA1 protein is a cytoplasmic protein, encoded by the single-gene operon and has been linked to divalent cation tolerance. It has no recognized structural motifs. This family also contains putative proteins from eukaryotes (human and Drosophila).	3.96078e-44
CP020114.1|AVZ30525.1|2504505_2505576_-|hypothetical-protein	gnl|CDD|276809	sd00006, TPR, Tetratricopeptide repeat. The Tetratricopeptide repeat (TPR) typically contains 34 amino acids and is found in a variety of organisms including bacteria, cyanobacteria, yeast, fungi, plants, and humans. It is present in a variety of proteins including those involved in chaperone, cell-cycle, transcription, and protein transport complexes. The number of TPR motifs varies among proteins. Those containing 5-6 tandem repeats generate a right-handed helical structure with an amphipathic channel that is thought to accommodate an alpha-helix of a target protein. It has been proposed that TPR proteins preferentially interact with WD-40 repeat proteins, but in many instances several TPR-proteins seem to aggregate to multi-protein complexes.	2.84695e-05
CP020114.1|AVZ30536.1|2518864_2519272_-|hypothetical-protein	gnl|CDD|377631	pfam06228, ChuX_HutX, Haem utilisation ChuX/HutX. This family is found within haem utilisation operons. It has a similar structure to that of pfam05171. pfam05171 usually occurs as a duplicated domain, but this domain occurs as a single domain and forms a dimer. The organisation of the dimer is very similar to that of the duplicated pfam05171 domains. It binds haem via conserved histidines.	1.13432e-06
CP020114.1|AVZ30534.1|2516162_2517983_-|lipid-A-export-ATP-binding/permease-protein-MsbA	gnl|CDD|224055	COG1132, MdlB, ABC-type multidrug transport system, ATPase and permease components [Defense mechanisms].	5.3731e-137

Protein sequences

>CP020114.1|AVZ30533.1|2514438_2515605_-|tyrosine-recombinase-XerC
MHKPEKASKGSAGIESFQGRLRLRLPRQLYEGSQKYLTLGLADTPENHHIAEQTAHQIELDILSGEFDKTLTKYKPQRHLSIVIPGEQQTITTISALWDKYIEYKRPSLKPRTLDKLAVLEKHIKRCRYQNLNESIKIKLALLQQTTNSQAKDVLMYLAAACKWGMKYGLVNFNPFEGMYNSLPKHNWQDNSKPNAFSEAEKQRIIQAFKNHKPLKGMGYSYYASFVEFLFLTGCRPSEAIGLQWKHITSDCSRITFDSALVQVGNGERVRVNGSKNNKMRGFNCNQKLQALLLSIKPENSEPESLVFPSPEGLSIHYRNFSRRAWDKIVDPLVARQTTPYSCRDTFISEQIAKGVPTAVVAKWCDNSVDVIEQKYLDAGLLDQLKPL
>CP020114.1|AVZ30532.1|2513299_2513959_-|hypothetical-protein
MFILKRQDVEISSIQHPKRDQQVSILNYQGQTFRLISVFKASQEEEARALWRELTDNRGKACVLLEEPDRFSVWGKVRLDQIDNDTGGYSKRGIYIQGSLLLLQAVYLDIEELLGNRQAGIFEKDIAQVLQEQKFPETSSLEAVKDLVTNDPLDLVKLPPWQEEHLISLLQELHKLGKTYFGNTNFAHQVTDMLQDMPEAERSLFITWLNQTSLSKLWQ
>CP020114.1|AVZ30531.1|2512309_2513161_-|hypothetical-protein
MSSSYDKSYRSPSLFTNRNIILAGTAWAVLALLYFLLFSAKVPGADGVESRADWYVIGTNIFEGLAYLGAGILCLRNWRSPQIVSSRNIWLAIAIGMFSYFLGGIFFGYTEIILDEEPDVSIGDIFFVLTYLSLGVGMILAVASRRINLEKWQLLIVWAIGVFGSLLPWWISMKESEPSEPLVTVLSWFYIVSDVVLLIIATTLLLAFWGGRVAQSWRMIAAAAFSLYIADMWFNYAQGPNYQSGEILEVFWVFSGVLFGMGAVLEHDASLSRTRRSSGRKRA
>CP020114.1|AVZ30530.1|2511293_2512292_-|hypothetical-protein
MRKLTDPDKQEILKLYRETAETTSTLAERYGVSNSTISRLLKSTLPEDEYEYLVSLKRAARTPEGRAQVSYEQLPLLTQPEPEKAQPSQEKATPRLKLPEISPPKPVVEEIHPESELEEEISSSIRRVRRRSSAEEKPKLRPAKRLEIVEPKPPEITSVPGPILKDEHPEANVIAQMLGEDLLDESEDLSDLEDDLDEDDYEDDYEDEEEDLEDSTPLVTTRRVSEAPVQVLPLSAAQLPKSCYLVIDRSSELITRPLKDFGDLGQIPNLETLQKTLPVFDNHRVAKRFSTKRDRVIKVPDSKVLHKASTHLQAKGITRLLIDGQVYSLSMV
>CP020114.1|AVZ30529.1|2510961_2511288_-|divalent-cation-tolerance-protein-CutA
MEIPADYGVVLVTTANKQEAETIANALVEAQLAACVSLLPIHSIYAWQGEIYKEYEWQLLIKTDLALFSTLEAKIRELHSYEVSEIIALPIVAGSQAYLQWISQQVKP
>CP020114.1|AVZ30528.1|2508663_2509200_-|hypothetical-protein
MKNILKSFVAISALSSLIVAPLIINAGQASAQPKKGTDASYVGIGVTGGLTNDGGNQDTGNFGGNVTGRVKLGNTPLSARGNVIWNNETSAIIPEVSVDVPIAKGTNAFITGGYSFLEKNNLPTPLGDKDAVVVGAGVESEVAKNLLVYTNAKVGIRSYENSGGSAVSINGGVGYRFR
>CP020114.1|AVZ30527.1|2506525_2508445_-|sulfite-reductase
MVKSAPAPIANRDQPSKVEGIKERSNFLREPVATQILEDTTHFTEEAVQLIKFHGSYQQDNRDNRVKGQEKDYQFMLRTRNPGGFVPPQLYLALDNLSDEHGNHTLRVTTRQGFQLHGILKKNLKAAIATIVQNMGSTLGACGDLNRNVMAPPAPFKNRRDYQYAWEYANNIADLLTPQTGAYYEIWLDGEKIISAEESPEVKAARQRNGTGTIIHDNEEPIYGTHYMPRKFKISVTVPGDNSVDLYSQDLTLVVITNPLGELEGFNVFAGGGFGRTHNKEETFARIADEICYVDKDDVYQLVKAIVATQRDYGDRTDRRHARLKYLINEWGVDKFRTQVEDYFGKSVAPFKPLPEFKYYDFLGWNEQGDGKLFLGISVDNGRVKDEGSFQLKTALRKVVEQFHLPMRLTPHHNIIFYDIQPENKSAIQEILQSCGIVDDPETIEPLVRYAMACPALPTCGLAITESERAIPGILERIRTLLDKLGLQKEHFVVRMTGCPNGCARPYMAELGFVGSAPESYQVWLGGSPDQTRLAQPYIEKLHHNDIESFLEPIFVYFKNFREYEESFGDFCDRLGFDALREFAATYQPQTTNTVSKSRHRVSIKDEVYLKLKEAANSQDRPMTELVNEALEAYFQNLP
>CP020114.1|AVZ30526.1|2505716_2506424_-|ribosomal-RNA-small-subunit-methyltransferase-E
MAQLQRIAIAPSQIQENLITLTKEQQHYLVRVLRLRTGDRFIAMDGKGKWWLTQLESEQAQVLETLTVETELSVAITLMVALPKGNGFDDIIRYSTELGVTCIAPLLSDRTLLNPSPQKLERWRRIATEAAEQSERAFVPTILEPVSFMTAINASQATHRYICEGRGDYPHLNSLVKTDIPEIIIATGPEGGWTEPEIENAIASGFQPVSLGRRILRAVTAPVVALTLISANYEV
>CP020114.1|AVZ30525.1|2504505_2505576_-|hypothetical-protein
MIEQLATAFERKDYHTATKLLKQLLKETPENPWVQFYLGLLHQVSGKHQQADKIYRHLLRSTTNTKILAQARQGLQRLQEIEQEERQRAISKATSEPSNAELGVLVLEPLATELKTIAAPKFAQIMQLDPYTARLLLPSRGWRFYRTGQIGELKFYGQQLQQASIPCFWTTIAAVEQIQVFQLKYFSESQPKPTIVCRNQENQLGSLTFDWSEVTAKVVGRLPMFEQVIDLDKRGRLERKTETQDYAEFCDLHLPSRNCILRFYDHGYEFQKGVEMTSQASQNTTRINWNSLLQWISSQIPEVKTWSNFTSFAQTVLDQTEMLGNIESHIHLLRREKTNWDPAFHLYSGLVFVNQT
>CP020114.1|AVZ30524.1|2502920_2503589_-|extracellular-esterase-EstB
MNTKNQQRNSVLLIHGIDDTGAVFNQMASYLRQLGWSVHTLDLVPNNGAVGLDILAQQIADYVVKTFAPEEPIDLVGFSMGGIVSRYYVQRLGGINRVQRFITISSPHHGTVTAYASQRHGCLQMRRNSQFIQDLNADAVMLGQLNFTSIWTPYDLMIVPANSSQMPLGKEVVVPVVLHSWMLTDSRSLAAVASALAEPVNPCHQFGYADNCQKSPLGGGNI
>CP020114.1|AVZ30534.1|2516162_2517983_-|lipid-A-export-ATP-binding/permease-protein-MsbA
MAKFQDILNYFHSDWKLSVFTIAASSVYEIIDLLVPYGIGQILNVLSNQPLDKPLQSAIASIAEYINYPVNQTLSLGVLLGLIFVVTVVKAPTQPWLTVWFHWDIPLRARRHQNETAIAKILTLPLEFYDENNPGRISGRVARGLANHTWTYPEIAGQLIPKLFRVLGIFGFILLIEWRIAVLYLISFVFILAFSLKTLRRIIWHESRLDKYMEGTESRTSELITNIKTVKAFATESQELKRQKQRLERELTVVDHRIHKGYVKLLTLQKVVIQFCVFTILGLTLAATVAGRITLGHFVMTLTLSSMAYAELEPISTLAELFARRYSSMLRFHEFLQAPIASDSVGLLEEEKQKESPYKFTGKIELSHINFGYDDSRQVLQDINLLISPYQTVALVGRSGSGKSTLVKLLLRYFEPQEGQISIDGQDIRTLDVGKYRRRLAIVHQEVDIFNGTILDNLTYGKPNATLEEVKEACRIARVDEVMSHLPQGYYTVVGERGVRLSGGQRQRLGIARALLVEPDVLIFDEATSSLDYESERAIQLAMRSIQGTCTTIVIAHRLSTVREADKIVVLDQGRIAEVGSHDELLRHEGIYHRLHSLQETGELLS
>CP020114.1|AVZ30535.1|2518070_2518859_-|hypothetical-protein
MLNIRAIWSCCLIIFCLLGSSGTAKAGELSQRLANFPQWEQLTSVQPAQGDLVYPDWFAGSWEVTSTLVDLVAPLAPDIITPGFESNRQQLNQPVSFLVRFIPEKSPITAWKFIPKIVNKSSILVADRAFNSLNLARAYLGDEAVLSTKVDPESPNRQITFLRGDSQLISIVTARLTETTSNRNFITSEVFQQLFKGGSRPYFNTVESTTAYHQLSTSYPSIEADQVTAVYLSPQDPDYFAAASHPVALYRYRLEFSPTSQD
>CP020114.1|AVZ30536.1|2518864_2519272_-|hypothetical-protein
MNNNLKDFLEACENLGTLRIIVTSSAAVLEARGKIEKLFYAELLKGKYVNLHTEGFEFHFNMDKITQVKFETGNAKRGNFTTYAIRFLDETEEVALSLFLQWGKPGEYEPGQVEAWQNLREQYGEVWQPVPIETL
>CP020114.1|AVZ30537.1|2519560_2520514_-|PhoH-like-protein
MADALIIQLPNIPSAIALAGDREENLKLLSRQTGASLVLRGQELHIGGTEAQMHLADQLVRSLEVIWTKGNTLSSADILTARQALDSDRQDELQELQRDVLAKTRRGEEIRAKTFRQKEYIKDIRRRDLTFCIGPAGTGKTYLAVVVAVQALLNNQVEKLILTRPAVEAGEKLGFLPGDLQQKVNPYLRPLYDAIYEFIDPEKVPSLMERGVIEVAPLAYMRGRTLNHAFVIVDEAQNTTPAQMKMVLTRLGFRSRMVITGDTTQTDLPLSQKSGLAVALQVLKNVEGIGFCEFTQKDVVRHALVQRIVAAYERYER
>CP020114.1|AVZ30538.1|2520647_2521106_-|hypothetical-protein
MFLNRSVPQPHLNAGIESLTASPNYVRLVKFLMQPFLESPETLSIDCEISQTLKRVWIRIAFESKDKGKVFGRGGRNIQAIRTVIAAAAEFAGQSVYWDMYGSNSFGREGMSSDDDQQERSPSALRGGQSPEPKTPDRTVNIPKPVVKPRIR
>CP020114.1|AVZ30539.1|2521068_2521329_-|30S-ribosomal-protein-S16
MIKLRLKRFGKKREPSYRIIAINSLARRDGRPLEELGFYNPITDEVRLDVPGIVKRLQQGAQPTETVRSILVKANVFEQVSATTAS
>CP020114.1|AVZ30540.1|2521446_2522919_-|signal-recognition-particle-protein
MFDALSDRLESAWKKLRGQDKISQSNIQDALREVRRALLEADVNLQVVKDFISEVETKAQGADVVAGVRPDQQFIKIVHDELVRVMGAENVPLAETEEKPTIVLMAGLQGTGKTTATAKLALHLRKLERSCLLVATDVYRPAAIDQLVTLGKQIDVPVFELGSDADPVEIARQGVERAKAEGFNTVIIDTAGRLQIDEDMMGELARIKATVQPHETLLVVDSMTGQEAANLTRTFHDKIGITGAILTKLDGDTRGGAALSVRQISGAPIKFVGVGEKVEALQPFYPERMASRILGMGDVLSLVEKAQEEFDLADAEKMQDKILSAKFDFNDFVKQLRMLKNMGSLGGIMKLIPGMNKISDEQLKQGETQLKRCESMINSMTRQERQDPDLLASSPSRRRRIAKGSGYREPDVSKLVADFQKMRTLMQQMGQGQMPAGMPGMFGGGGMGNPFGGGDNSRPSSPGWRGYNSGDRTTKKKKNKEKKKKGFGTL
>CP020114.1|AVZ30541.1|2523711_2525067_-|Xaa-Pro-aminopeptidase
MLKQTLHHRRQQLATLINFPAILWSGNTNPRNFPANTYPFRASSHFLYFAGLPLQNAAIRLEGGKLELFIDNPTPSSALWHGQMPTRDEIAEQIGADTARPMAELESYLEDAATIPVQNAATWTQQTQLLNRWVLPQQPPQGNDLELAKAIISLRLTHDAGALIELRKAAAVTVAAHKAGIAATSKAKLEAEVRAAMEAVIIGENMTTSYNSIVTVHGEVLHNEHYHHPLQPGDLILADVGAETAMGWAADVTRTWPVSGKFSSTQRDIYDVVLAAHDACIAKIRPGVEYGDIHLLAATVIAEGLVDLGILQGNPADLVEMDAHALFFPHGIGHLLGLDVHDMEDLGDLAGYEEGRTRSDRFGLGYLRLNRLLRPGMLVTIEPGFYQVPAILNDPNVRSKYENIVNWQRLSEFTDVRGIRIEDDVLVTESGSEVLTDALPNQAIAIETIAN
>CP020114.1|AVZ30542.1|2526508_2527123_-|HTH-type-transcriptional-repressor-KstR2
MRVFNSPPPSEAQTRTRILQAAQKLFASKGFDGTTTRDLAQAAGVAEGTLFRYFANKKSILVEVATSGWVDILTDLLTELSEMGSYKAIAQVMRRRMWNLHKNVDLMKVCFMEVQFHPDLRDRIQEEVIGKMTDVAEAFFQSAMDKGIYRQTDAKLVAKVFLGMFAIAGFSNNTLMAPDASPQEMQQMAEGLADIFLNGVLAKE
>CP020114.1|AVZ30543.1|2539229_2540651_-|isocitrate-dehydrogenase
MYDKIIPPTTGTKITFKNGEPIVPDNPIIPFIRGDGTGIDIWPATQKVLDAAVAKAYKGQRQISWFKVYAGDEACDLYGTYQYLPQDTLTAIKEYGVAIKGPLTTPVGGGIRSLNVALRQIFDLYACVRPCRYYAGTPSPHKNPEKLDVIVYRENTEDIYLGIEWKQGSEIGDRLIKILNEELIPATPEHGKKQIPLDAGIGIKPISKTGSQRLVRRAMKHALLLPKDKQQVTLVHKGNIMKYTEGAFRDWGYELVTSEFRSQCVTERESWILINKEKNPDISPEDNAREVDPGYDSLTPEKKAQIVQEVETVLSTIWETHGNGQWKAKIMVNDRIADSIFQQIQTRPDEYSILATMNLNGDYLSDAAAAIVGGLGMGPGANIGDACAVFEATHGTAPKHAGLDRINPGSVILSGVMMLEFMGWQEAADLVKKGLGQAIAHSQVTYDLARLLEPPVEPLKCSEFADAIIKHFD

Crispr_ID: CP020114_11

• CRISPR_ID: CP020114_11
• CRISPR_location: 2651429-2651528
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 1 spacers

Consensus repeat of CP020114_11

Consensus_repeat	Method
ATCTCCCATTCCTGCATTAGAACCAGAG	CRISPRCasFinder

spacers of CP020114_11

>11.1|2651457|44|CP020114|CRISPRCasFinder
TTTATCCCAGAAGATAATATTGCAGAGGCTACACCTGCATCTGT

CRISPR arrays and Neighbor proteins around CP020114_11

CRISPR arrays

The CRISPR arrays of CP020114_11

>merge|CP020114|11|2651429-2651528|CRISPRCasFinder
ATCTCCCATTCCTGCATTAGAACCAGAGTTTATCCCAGAAGATAATATTGCAGAGGCTACACCTGCATCTGTATCTCCCATTCCTGCATTAGAACCAGAG

>CP020114|11|11|2651429-2651528|CRISPRCasFinder
ATCTCCCATTCCTGCATTAGAACCAGAG	TTTATCCCAGAAGATAATATTGCAGAGGCTACACCTGCATCTGT
ATCTCCCATTCCTGCATTAGAACCAGAG

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
CP020114.1|AVZ30585.1|2656759_2657509_-|GDP-mannose-4,6-dehydratase	unknown	unknown	gnl|CDD|224014
CP020114.1|AVZ30580.1|2647596_2648322_-|hypothetical-protein	unknown	unknown	gnl|CDD|226692
CP020114.1|AVZ30579.1|2646479_2647592_-|nicotinate-nucleotide--dimethylbenzimidazole-phosphoribosyltransferase	unknown	unknown	gnl|CDD|235300
CP020114.1|AVZ30582.1|2654304_2655132_-|putative-chitobiose-transport-system-permease-protein	unknown	unknown	gnl|CDD|223472
CP020114.1|AVZ30591.1|2671002_2671572_-|HTH-type-transcriptional-regulator-GltC	unknown	unknown	gnl|CDD|223656
CP020114.1|AVZ30589.1|2663945_2664509_-|hypothetical-protein	unknown	unknown	gnl|CDD|368559
CP020114.1|AVZ30583.1|2655151_2655697_-|5-formyltetrahydrofolate-cyclo-ligase	unknown	unknown	gnl|CDD|274270
CP020114.1|AVZ30576.1|2639053_2639716_-|riboflavin-synthase	unknown	unknown	gnl|CDD|236455
CP020114.1|AVZ30573.1|2633296_2634130_-|3',5'-cyclic-adenosine-monophosphate-phosphodiesterase-CpdA	unknown	unknown	gnl|CDD|224326
CP020114.1|AVZ30574.1|2634228_2635059_-|3',5'-cyclic-adenosine-monophosphate-phosphodiesterase-CpdA	unknown	unknown	gnl|CDD|224326
CP020114.1|AVZ30581.1|2648399_2649101_-|cAMP-activated-global-transcriptional-regulator-CRP	unknown	unknown	gnl|CDD|223736
CP020114.1|AVZ30575.1|2635124_2635895_-|laccase-domain-containing-protein	unknown	unknown	gnl|CDD|224413
CP020114.1|AVZ30586.1|2657900_2658683_-|undecaprenyl-phosphate-N,N'-diacetylbacillosamine-1-phosphate-transferase	unknown	unknown	gnl|CDD|274395
CP020114.1|AVZ30577.1|2643082_2643283_-|hypothetical-protein	unknown	unknown	unknown
CP020114.1|AVZ30588.1|2663433_2663949_-|CDP-diacylglycerol--glycerol-3-phosphate-3-phosphatidyltransferase	unknown	unknown	gnl|CDD|273139
CP020114.1|AVZ30587.1|2658792_2659938_-|GDP-mannose-dependent-alpha-(1-6)-phosphatidylinositol-monomannoside-mannosyltransferase	unknown	unknown	gnl|CDD|340833
CP020114.1|AVZ30584.1|2655791_2656736_-|GDP-L-fucose-synthase	unknown	unknown	gnl|CDD|187550
CP020114.1|AVZ30578.1|2644284_2645484_-|spermidine/putrescine-binding-periplasmic-protein	unknown	unknown	gnl|CDD|270379
CP020114.1|AVZ30572.1|2629789_2630887_-|tocopherol-cyclase	unknown	unknown	gnl|CDD|372979
CP020114.1|AVZ30590.1|2670749_2671022_-|anthranilate-phosphoribosyltransferase	unknown	unknown	gnl|CDD|168934

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
CP020114.1|AVZ30585.1|2656759_2657509_-|GDP-mannose-4,6-dehydratase	gnl|CDD|224014	COG1089, Gmd, GDP-D-mannose dehydratase [Cell envelope biogenesis, outer membrane].	4.06712e-149
CP020114.1|AVZ30580.1|2647596_2648322_-|hypothetical-protein	gnl|CDD|226692	COG4241, COG4241, Predicted membrane protein [Function unknown].	1.62198e-57
CP020114.1|AVZ30579.1|2646479_2647592_-|nicotinate-nucleotide--dimethylbenzimidazole-phosphoribosyltransferase	gnl|CDD|235300	PRK04447, PRK04447, hypothetical protein; Provisional.	7.13026e-173
CP020114.1|AVZ30582.1|2654304_2655132_-|putative-chitobiose-transport-system-permease-protein	gnl|CDD|223472	COG0395, UgpE, ABC-type sugar transport system, permease component [Carbohydrate transport and metabolism].	1.31202e-88
CP020114.1|AVZ30591.1|2671002_2671572_-|HTH-type-transcriptional-regulator-GltC	gnl|CDD|223656	COG0583, LysR, Transcriptional regulator [Transcription].	8.5143e-38
CP020114.1|AVZ30589.1|2663945_2664509_-|hypothetical-protein	gnl|CDD|368559	pfam05685, Uma2, Putative restriction endonuclease. This family consists of hypothetical proteins that are greatly expanded in cyanobacteria. The proteins are found sporadically in other bacteria. A small number of member proteins also contain pfam02861 domains that are involved in protein interactions. Solutions of several structures for members of this family show that it is likely to be acting as an endonuclease.	1.66503e-31
CP020114.1|AVZ30583.1|2655151_2655697_-|5-formyltetrahydrofolate-cyclo-ligase	gnl|CDD|274270	TIGR02727, Uncharacterized_protein_YqgN, 5,10-methenyltetrahydrofolate synthetase. This enzyme, 5,10-methenyltetrahydrofolate synthetase, is also called 5-formyltetrahydrofolate cycloligase. Function of bacterial proteins in this family was inferred originally from the known activity of eukaryotic homologs. Recently, activity was shown explicitly for the member from Mycoplasma pneumonia. Members of this family from alpha- and gamma-proteobacteria, designated ygfA, are often found in an operon with 6S structural RNA, and show a similar pattern of high expression during stationary phase. The function may be to deplete folate to slow 1-carbon biosynthetic metabolism. [Central intermediary metabolism, One-carbon metabolism].	2.87851e-56
CP020114.1|AVZ30576.1|2639053_2639716_-|riboflavin-synthase	gnl|CDD|236455	PRK09289, PRK09289, riboflavin synthase.	6.43374e-89
CP020114.1|AVZ30573.1|2633296_2634130_-|3',5'-cyclic-adenosine-monophosphate-phosphodiesterase-CpdA	gnl|CDD|224326	COG1408, COG1408, Predicted phosphohydrolases [General function prediction only].	2.08636e-65
CP020114.1|AVZ30574.1|2634228_2635059_-|3',5'-cyclic-adenosine-monophosphate-phosphodiesterase-CpdA	gnl|CDD|224326	COG1408, COG1408, Predicted phosphohydrolases [General function prediction only].	4.61059e-68
CP020114.1|AVZ30581.1|2648399_2649101_-|cAMP-activated-global-transcriptional-regulator-CRP	gnl|CDD|223736	COG0664, Crp, cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases [Signal transduction mechanisms].	1.88724e-47
CP020114.1|AVZ30575.1|2635124_2635895_-|laccase-domain-containing-protein	gnl|CDD|224413	COG1496, yfiH, Multicopper polyphenol oxidase (laccase) [Secondary metabolites biosynthesis, transport and catabolism].	9.13106e-71
CP020114.1|AVZ30586.1|2657900_2658683_-|undecaprenyl-phosphate-N,N'-diacetylbacillosamine-1-phosphate-transferase	gnl|CDD|274395	TIGR03022, WbaP_sugtrans, Undecaprenyl-phosphate galactose phosphotransferase, WbaP. The WbaP (formerly RfbP) protein has been characterized as the first enzyme in O-antigen biosynthesis in Salmonella typhimurium. The enzyme transfers galactose from UDP-galactose to a polyprenyl carrier (utilizing the highly conserved C-terminal sugar transferase domain, pfam02397) a reaction which takes place at the cytoplasmic face of the inner membrane. The N-terminal hydrophobic domain is then believed to facilitate the "flippase" function of transferring the liposaccharide unit from the cytoplasmic face to the periplasmic face of the inner membrane. This model includes the enterobacterial enzymes, where the function is presumed to be identical to the S. typhimurium enzyme as well as a somewhat broader group which are likely to catalyze the same or highly similar reactions based on a phylogenetic tree-building analysis of the broader sugar transferase family. Most of these genes are found within large operons dedicated to the production of complex exopolysaccharides such as the enterobacterial O-antigen. The most likely heterogeneity would be in the precise nature of the sugar molecule transferred.	1.46956e-93
CP020114.1|AVZ30588.1|2663433_2663949_-|CDP-diacylglycerol--glycerol-3-phosphate-3-phosphatidyltransferase	gnl|CDD|273139	TIGR00560, pgsA, CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase. Alternate names: phosphatidylglycerophosphate synthase; glycerophosphate phosphatidyltransferase; PGP synthase. A number of related enzymes are quite similar in both sequence and catalytic activity, including Saccharamyces cerevisiae YDL142c, now known to be a cardiolipin synthase. There may be problems with incorrect transitive annotation of near homologs as authentic CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase. [Fatty acid and phospholipid metabolism, Biosynthesis].	1.62392e-45
CP020114.1|AVZ30587.1|2658792_2659938_-|GDP-mannose-dependent-alpha-(1-6)-phosphatidylinositol-monomannoside-mannosyltransferase	gnl|CDD|340833	cd03804, GT4_WbaZ-like, mannosyltransferase WbaZ and similar proteins. This family is most closely related to the GT4 family of glycosyltransferases. WbaZ in Salmonella enterica has been shown to possess mannosyltransferase activity.	2.77723e-177
CP020114.1|AVZ30584.1|2655791_2656736_-|GDP-L-fucose-synthase	gnl|CDD|187550	cd05239, GDP_FS_SDR_e, GDP-fucose synthetase, extended (e) SDRs. GDP-fucose synthetase (aka 3, 5-epimerase-4-reductase) acts in the NADP-dependent synthesis of GDP-fucose from GDP-mannose. Two activities have been proposed for the same active site: epimerization and reduction. Proteins in this subgroup are extended SDRs, which have a characteristic active site tetrad and an NADP-binding motif, [AT]GXXGXXG, that is a close match to the archetypical form. Extended SDRs are distinct from classical SDRs. In addition to the Rossmann fold (alpha/beta folding pattern with a central beta-sheet) core region typical of all SDRs, extended SDRs have a less conserved C-terminal extension of approximately 100 amino acids. Extended SDRs are a diverse collection of proteins, and include isomerases, epimerases, oxidoreductases, and lyases; they typically have a TGXXGXXG cofactor binding motif. SDRs are a functionally diverse family of oxidoreductases that have a single domain with a structurally conserved Rossmann fold, an NAD(P)(H)-binding region, and a structurally diverse C-terminal region. Sequence identity between different SDR enzymes is typically in the 15-30% range; they catalyze a wide range of activities including the metabolism of steroids, cofactors, carbohydrates, lipids, aromatic compounds, and amino acids, and act in redox sensing. Classical SDRs have an TGXXX[AG]XG cofactor binding motif and a YXXXK active site motif, with the Tyr residue of the active site motif serving as a critical catalytic residue (Tyr-151, human 15-hydroxyprostaglandin dehydrogenase numbering). In addition to the Tyr and Lys, there is often an upstream Ser and/or an Asn, contributing to the active site; while substrate binding is in the C-terminal region, which determines specificity. The standard reaction mechanism is a 4-pro-S hydride transfer and proton relay involving the conserved Tyr and Lys, a water molecule stabilized by Asn, and nicotinamide. Atypical SDRs generally lack the catalytic residues characteristic of the SDRs, and their glycine-rich NAD(P)-binding motif is often different from the forms normally seen in classical or extended SDRs. Complex (multidomain) SDRs such as ketoreductase domains of fatty acid synthase have a GGXGXXG NAD(P)-binding motif and an altered active site motif (YXXXN). Fungal type ketoacyl reductases have a TGXXXGX(1-2)G NAD(P)-binding motif.	1.23117e-153
CP020114.1|AVZ30578.1|2644284_2645484_-|spermidine/putrescine-binding-periplasmic-protein	gnl|CDD|270379	cd13661, PBP2_PotD_PotF_like_1, The periplasmic substrate-binding component of an uncharacterized active transport system closely related to spermidine and putrescine transporters; contains the type 2 periplasmic binding fold. This group represents the periplasmic binding domain that serves as a primary polyamine receptor of an uncharacterized ABC-type transport system from plants and plant-symbiotic cyanobacteria. Polyamine transport plays an essential role in the regulation of intracellular polyamine levels which are known to be elevated in rapidly proliferating cells and tumors. Natural polyamines are putrescine, spermindine, and spermine. They are polycations that play multiple roles in cell growth, survival and proliferation, as well as plant stress and disease resistance. They can interact with negatively charged molecules, such as nucleic acids, to modulate their functions. Members of this family belong to the type 2 periplasmic-binding fold superfamily. PBP2 is comprised of two globular subdomains connected by a flexible hinge and bind their ligand in the cleft between these domains in a manner resembling a Venus flytrap.	5.66455e-113
CP020114.1|AVZ30572.1|2629789_2630887_-|tocopherol-cyclase	gnl|CDD|372979	pfam14249, Tocopherol_cycl, Tocopherol cyclase. This family contains tocopherol cyclases. These enzymes are involved in the synthesis of tocopherols and tocotrienols (vitamin E).	0
CP020114.1|AVZ30590.1|2670749_2671022_-|anthranilate-phosphoribosyltransferase	gnl|CDD|168934	PRK07394, PRK07394, hypothetical protein; Provisional.	1.59308e-35

Protein sequences

>CP020114.1|AVZ30581.1|2648399_2649101_-|cAMP-activated-global-transcriptional-regulator-CRP
MEDRYSLQATSNAWMISSAPFFQGLPAAAIELALTHLVTRTHPANQVILLENDWGGAVYFILDGWVKIRTYNMEGKEVTLNIIGKGELFGEMGALDEVPRSTDVITLTSTMIGSMPSQDFVKLLHTEPLAGLRLSQLMARRLRQVNRRLRLRESDSQSRVADTLLFLAEGQGTQGQTGTEIPNLPHRELSSLSGLARETVTRVLTRLEKKGLITKYPEIICIPDLSALEKMIV
>CP020114.1|AVZ30580.1|2647596_2648322_-|hypothetical-protein
MSISDSLPDENPSPEFPNSLNKHLEPEQLTRPRLKVDAPLRMVETAFLASAASLIWFINFYFPLGPVLRIFFPVPIALVYLRWGKRAAWMAAVTSGLLLSVLMGPVRSLLFVMPFAFMGVLLGATWHRRVPWIVSITLGTLLGTLGVFFRLWLLSVLSGEDLWVYVINQVTELIEWAFLRLELLASPSLFLIQVGAVALIVLNNFIYLFMVHLAAWLLLDRLGNPIPRPPEWVQVLMDYEG
>CP020114.1|AVZ30579.1|2646479_2647592_-|nicotinate-nucleotide--dimethylbenzimidazole-phosphoribosyltransferase
MIRIYTQIKQGEEWIAKYRECLPVFGCVLGFTDTGLIPGISAAGSTPEDRKYTACADAEFLYYGAGRKPLYPLPPLTAGASPVLISRAVVEALNIPVYLFNAGLPQPPAVPVIDLGGSPAKCLSEGAAMNITTVHHLLKQGLLWGERLAANIPQGYVILGECVVGGTTTALGILTGLGVDAVGKVNSSHPVCNHTQKWAVVQAGLERMRASGGKLLDPLEIVAAVGDPMQVVVAGMAIAASRNCGVLLAGGTQMLAVYALISAIADTYDLSWQPTEVVVGTTRWVAEDPTGSTVDLALSLGKTIPPLLATQLSFANSRYPQLQAYEQGFVKEGIGAGAACIAASLRQNWQQHQLLTAIEAQLERLKSVNS
>CP020114.1|AVZ30578.1|2644284_2645484_-|spermidine/putrescine-binding-periplasmic-protein
MYNPESRIQNPKSMDRRSFLLGASGLALSQFLFGCGDSNQTKLNIQLLKGSIPSQIVRQFRQSLQQQVQLNFSPVEQIQDLFKQLQGWQDKSKTTDKPFWTRFIPFRKSPPAVASDLVTLGDYWLKAAIEQKLIQPLEEAEIKQLKHWSALDKTWQQLVTRDNQGNLDTQGNVWAAPYRWGSTVIVYNRQKLQSLGWTPQDWSDLWRDGLQERISIVDQSREVIGLVLKKLGESYNTENLDTIPNLENELRALNQQVKFYSSNNYLEPLIIGDTWLAVGWSSDIIPVLARYPQLAAVIPKSGTAIWADLWVRPTGVKKDTFSSQWIDFCWQPNIAKQISLLTRSNSPTFPNITSSERQEAFRSLLQSNREVFDQSEFLHSLPLLAIEQYESLFAKIKQG
>CP020114.1|AVZ30577.1|2643082_2643283_-|hypothetical-protein
MTSQEFLENLATAATDPEKLMVVAEYLETTAMDNATTPRWRSIPYSSEIEMALKNLAFHLEGLAET
>CP020114.1|AVZ30576.1|2639053_2639716_-|riboflavin-synthase
MFTGLIQALGMMKPLGGDSWQITFVSQSSAVIMQDLAYGDSVAVDGVCLTVEKILNNGFIATASPETLRRTTLGEEETQQGYVNLEASLRVGGKVGGHFVMGHVDGIGQLVTAKQTASSWEMTFTAPEAIARYIVPKGSIAVNGISLTVAAYEPELSQFTVAVIPLTYDDTNLRSLVSGSWVNLEGDILGKYVEKFLSPSKQHPQTDMVTPEFLAEHGYL
>CP020114.1|AVZ30575.1|2635124_2635895_-|laccase-domain-containing-protein
MHTWHWHNWQGLPYLTCSLLKDWHHGFFTQQFWPRSPEDLTKFMHPEASAYRLQQVHGNIVLTPQEIESQTRTEDSGLVPGDGLISQQPLQALWVASADCTPVLIGDVKTRQVAALHAGWRGTAAKIVPQAIARLRNQGSQLDDLRIAMGPAIAGEVYQVSVEVAAEVGASITPHTEEQQIIDALHELPNSPLLADPEPGKVRLDVRRVNVLQLESLGITAEQIAIAPYCTYQTPEYFFSYRREQQKKIQWSGIIM
>CP020114.1|AVZ30574.1|2634228_2635059_-|3',5'-cyclic-adenosine-monophosphate-phosphodiesterase-CpdA
MHWLLSGPLSIERLTVKIAGLPASLEGKKLVQMSDFHYDGLRLSEEMLEQAIAISNQLKPDLVLLTGDYVTTSTEPIHDLASRLKQLKSQAGIYAILGNHDIYYKHSKSEITNALTSIDINVLWNEIAYPLGEDLPLVGLADRYSPEFNPTVVMNQLNPDIPRIVLSHNPDTAELLQGWRVDLQLSGHTHGGQIVIPGLGPILVGYKKILQNVPQKVRYRFPCLRKNYLIIRHWEWSQGLHQVGNNQLYVNRGLGTYFPGRLFCPPEVTEIILQKA
>CP020114.1|AVZ30573.1|2633296_2634130_-|3',5'-cyclic-adenosine-monophosphate-phosphodiesterase-CpdA
MHWLFTGRLRVDKITVKIADLPQSLQGTKLVQLTDFHYDGVRISEELLAQAIAASNEAEPDIILLTGDYVTDDPTPIHELALRLKHLQSRCGIYAVLGNHDIHYKHSKTEITSALTSIGVHVLWNEIAYPLGQELPIIGLAEYWSREFNPAPVMDILNSDIPRIVLSHNPDTAKILQKWRVDLQLSGHTHGGHIVLPGLGPAIFHYQKLLKQLPKKLRRWEPLLLGDCGKVVKYWEWAKGFHQVSNNQLYVNRGLGTYRPGRLFCPPEVTVITLVSL
>CP020114.1|AVZ30572.1|2629789_2630887_-|tocopherol-cyclase
MLTIPLNLLQSTQTPHSGYHWDGSSRSFFEGWYYRVTLPHCGQTFAFMYSIEDPIGSKPYSGGAAQILGANDEYICRTFPDVKKFWGSPDVLGLGHWGKTDLHTPPLYLLPNEFERHIQSGYQATATLNQGEIHDPATGNYCRWLYEIQPIYYWGNQNSIQQSTAGWVSSLQIFEPGWQILMAHGLASGSIDWNGKIYEFTNAPAYSEKNWGGAFPQKWFWLNCNSFDGEPDLALTAGGGRRGVLWWMESVAMIGLHYQGKFYEFVPWNAKVEWDIQPWGRWQMRARNLDYEIELTGTTHLPGTPLRAPTANGLVFCCRDTMQGKLDLELRKLSSGKSSTILKAESSLCGLEIGGGSWDNSWHSK
>CP020114.1|AVZ30582.1|2654304_2655132_-|putative-chitobiose-transport-system-permease-protein
MPNSRLKIILIYGLLGAIALVMLFPLLWLISTALKSPTENILQSPPQLLPSQPTLNNFFTVWRSLPFAQYLYNSTLVAVLTVGLNVLFCALAAYPLARLSFVGRNAIFLAIVSTIMIPFQIVMIPLYILTVQLGLRNTYLGIIFPSLASAFGIFLLRQAFIGVPKELEEAARMDGSSELGLWWHIMLPAIRPALVTLSIFVFIGAWSDFLWPLIVIQDESLYTLPLGVAKLEGTFSLDWRLVAAGSIISIAPILLLFLFLQRYIVPTETGSGVKG
>CP020114.1|AVZ30583.1|2655151_2655697_-|5-formyltetrahydrofolate-cyclo-ligase
MGKVDLRRSLIQKRQSMSVGEWRERSDRICIQLQGSLLFRQAKTILAYFSFRQEPDLSLLFTDYHYNWGFPRCVGKSLDWHIWKPDDSLQVGAFGITEPLPHAPSITPAEVDLILVPCVACDYDKYRLGYGGGYYDRLLSSPDWANTPTIGVVFDFAYLSQLPIESWDKPLQSVISENYYT
>CP020114.1|AVZ30584.1|2655791_2656736_-|GDP-L-fucose-synthase
MTALELENNRILVTGGAGFLGRQVISQLCKAGADRAKITVTRSREHDLRVMENCQRAVDQQDIIIHLAAHVGGIGLNREKPAELFYDNLIMGTQLIHAAYQAGVAKFVCVGTICAYPKFTPVPFKEDDLWNGYPEETNAPYGIAKKALLVQLQSYRQQYGFNGIYLLPVNLYGPEDNFDPSSSHVIPALIRKVHEAQIKGEKQLPVWGDGSPTREFLYSEDAARGIVMGTISYNDSEAVNLGTGEEISIRDLITLICELMEFDGEIVWETNQPNGQPRRCLDTERAKQAFNFTAQVSFRQGLKNTIEWYRQNAV
>CP020114.1|AVZ30585.1|2656759_2657509_-|GDP-mannose-4,6-dehydratase
MGTLRLLEAIRDYQKRTGIQVRFYQAGSSEMFGLVQAVPQSETTPFYPRSPYACAKVYAHWQTVNYRESYNLFACNGILFNHESPRRGETFVTRKITRAVAKIVAGKQKKIYMGNLDSKRDWGYAKDYVRAMWLMLQQDQPEDYVIATGETYSVRQFLEKAFGYVNLKWEDYVKFDDRYIRPAEVDLLIGDPTKAKQKLGWEPSVTFDELVALMVEADLQALGHTSPNGNGIASDDMATIRQELGDLHF
>CP020114.1|AVZ30586.1|2657900_2658683_-|undecaprenyl-phosphate-N,N'-diacetylbacillosamine-1-phosphate-transferase
MTAQRSLLSGKRYLPFKNGKAQRQDTSASTRTFLRRGQKTKTPKLKPKGLSVQGLNGEFAKRLFDIVFSLLVLILFFPVYLILSLLIAFSSEGPIFYIQKRVGKNYKTFNCIKFRTMVSNADEMLMQMMATSPQLRQEFESSFKLKQDPRITKIGQFLRITSLDEFPQFWNVLKGDMSVVGPRPLVASELPKYGCHIDHILTIRPGITGLWQVSGRNDIPYPRRVQIDLHYVKFRNFWLDLWIIFKTIDVVIIPKNNGAY
>CP020114.1|AVZ30587.1|2658792_2659938_-|GDP-mannose-dependent-alpha-(1-6)-phosphatidylinositol-monomannoside-mannosyltransferase
MSLKYALVHEWLTPKATGGSELVVQEILNHIDANLYALIDFESSNPESYLYQRQIGTTFLQHLPYARNGVQKYLPLLPLAIEQLDLRQYEVILSSSHAVAKGVLTTPDQLHICYCHTPMRYAWDLTFDYLHQSKLGTGSAGWVTRYLLHRLRQWDVLSANRVDYFIANSQHTARRIWRCYRREATVIYPPVNVDNLPFLPHKADFYLTVSRLVSYKQVSLIVKAFNQLQLPLVIIGTGPEMPKIRELANSNIQILGWQPDDVVKKYMAEAKAFVYAACEDFGIALVEAQACGTPVIAYGAGGALETVRDLRSCMDTATGIFFRTQTEAAVMEAVEKFEIYQNAFNLEYMRSHASQFSLQVFAERYLKFIEQCNQEKTSSKR
>CP020114.1|AVZ30588.1|2663433_2663949_-|CDP-diacylglycerol--glycerol-3-phosphate-3-phosphatidyltransferase
MNLPNAITFSRLLGIPFLLYGLYNPTPQARWICLTIFLVAALTDWLDGYLARKLNQITDLGKFLDPLVDKLLVLAPLLVLVELERIPAWGVFVILARELAIAGWRVNQTTITGANIWGKLKTVSQIIAISLLIAPLSTLWETPSMIAFWISVALTLISGGIYLIPPAKNPT
>CP020114.1|AVZ30589.1|2663945_2664509_-|hypothetical-protein
MIKTPTRQITLAEFLQLPETEPASEYINGEIIQKPMPQGKHSTIQGELVTTINNVVKPRKIALAFPELRCTFGERSIVPDVAVFAWGRIPVDEKGNIANVFNTYPDWTIEILSPQQSTIKVTRNILHCLNHGTSLGWLIDPEEHNVLVYPHNQQPIFLENEQDILPVPELVGDFQLTLGQLFDCLKL
>CP020114.1|AVZ30590.1|2670749_2671022_-|anthranilate-phosphoribosyltransferase
MLLSPHDYGITSKNVPLGSTAELLTQIQEVLAGQPGELMQTALWNGGFYLWRSGICSDMPSGLSKAAELLHNGAVATKLQELRQSLSECH
>CP020114.1|AVZ30591.1|2671002_2671572_-|HTH-type-transcriptional-regulator-GltC
MRLEQLQAFLAIAQTGSFQQAARKCGVTQSTISRQIQSLEADLGVELFHRSSHAKLTLGGECLLPRVRKICQEWQTATQELTDLMEGKQPELCIAAIHSLCASYLPPVLQKFCHDYPEVQLRVTSLGSDRALKVLKDGLVDLAIVMNNRFLTTGKEMVVEVLYEESIEVLTAAHHPLAPRDRKIAALAP

Crispr_ID: CP020114_12

• CRISPR_ID: CP020114_12
• CRISPR_location: 4035453-4037321
• CRISPR_type: TypeIII-A
• Repeat_type: NA
• Spacer_info: 24 spacers
• Cas_protein_info: WYL,cas2,cas1,csm6,cas6,csm5gr7,csm4gr5,csm3gr7,csm2gr11,cas10

Consensus repeat of CP020114_12

Consensus_repeat	Method
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	CRISPRCasFinder
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	CRT
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	PILER-CR

spacers of CP020114_12

>12.1|4035490|36|CP020114|CRISPRCasFinder,CRT
CTCAAGAAAGCTTCACAGGGAAGTTTAGGTGTATTC
>12.2|4035563|39|CP020114|CRISPRCasFinder,CRT,PILER-CR
TAAACGCACCAAAAAACGGAGGTTGACGAAAGTGGACGA
>12.3|4035639|37|CP020114|CRISPRCasFinder,CRT,PILER-CR
GCGATCGCCCCAAAGCAAAGCCGGATTCAACAATCTA
>12.4|4035713|38|CP020114|CRISPRCasFinder,CRT,PILER-CR
GAAGTTTAGTAGGGATGCTTAGAAGCCCTAATAGGAAC
>12.5|4035788|38|CP020114|CRISPRCasFinder,CRT,PILER-CR
GTTGCTCAATTTATTAGAGAGTACAAATTAAAAGCCAA
>12.6|4035863|39|CP020114|CRISPRCasFinder,CRT,PILER-CR
TCATTATCCAAGATGGTGTGGGAGCATTTTAATGGCAAG
>12.7|4035939|46|CP020114|CRISPRCasFinder,CRT,PILER-CR
TAAACTCAGCCTTAAAAAGGTCAAACAGTTTTTTAATACCCTCCCA
>12.8|4036022|38|CP020114|CRISPRCasFinder,CRT,PILER-CR
AACTGAGGGGGTTAACCCCCTCAGTTTTCTCATACAAT
>12.9|4036097|46|CP020114|CRISPRCasFinder,CRT,PILER-CR
AGCAAATCGCTGTAGCACAGCTTGACACGGTTAAACACACTAAGGA
>12.10|4036180|39|CP020114|CRISPRCasFinder,CRT,PILER-CR
TTCCGTTATGGGTTACGAACTGATTAAAGCCACGAAAGG
>12.11|4036256|37|CP020114|CRISPRCasFinder,CRT,PILER-CR
ATCTAAAGTGGTCTGAGGGTAGTATTTATCCTCAACC
>12.12|4036330|42|CP020114|CRISPRCasFinder,CRT,PILER-CR
GATACAAGAGACACCCTTAATATCTTGTAATAACTGGGCATT
>12.13|4036409|38|CP020114|CRISPRCasFinder,CRT,PILER-CR
AGAAAAAACAATTGGATTTAAATATCCAAAGACAATAT
>12.14|4036484|39|CP020114|CRISPRCasFinder,CRT,PILER-CR
CTGATAACTGTTGGATAGCTGAGTGAGATCGCTCACAAG
>12.15|4036560|42|CP020114|CRISPRCasFinder,CRT,PILER-CR
CATTAGTTGAAGATGGTTCTGGACAAGGTTTAGGTCATCCTG
>12.16|4036639|41|CP020114|CRISPRCasFinder,CRT,PILER-CR
AATTAGGGTTTACACCTTTAAGAGAAAATGCTTTTATTTAT
>12.17|4036717|40|CP020114|CRISPRCasFinder,CRT,PILER-CR
GCTTTATGCAAGCTGCTTTGTTAGCCAGAAAAGAAAACCA
>12.18|4036794|39|CP020114|CRISPRCasFinder,CRT,PILER-CR
ATCACCTCATCTAGGATTACAGTTAAAGTGCCTTCTGAT
>12.19|4036870|39|CP020114|CRISPRCasFinder,CRT,PILER-CR
AAAGGGATGGCGACTTCTTTCCAATTATGTCTGCGTAGC
>12.20|4036946|39|CP020114|CRISPRCasFinder,CRT,PILER-CR
GAATGTTACAGAAAGCTTTGAAGAGTTTGGTATAACTGG
>12.21|4037022|38|CP020114|CRISPRCasFinder,CRT,PILER-CR
CAAGCAAAATTAGAAACAGTATTGGAAGCTGATGGCGT
>12.22|4037097|40|CP020114|CRISPRCasFinder,CRT,PILER-CR
CATATTAAGCTTTCAAATTTTCCTCTATTTTGGCTACAAC
>12.23|4037174|37|CP020114|CRISPRCasFinder,CRT,PILER-CR
CAAAGGTCAAGGACTTTTTGCAGGACGATATAAAATT
>12.24|4037248|37|CP020114|CRISPRCasFinder,CRT,PILER-CR
TAGGACTCCGCTTCTGTTTACAAGAAGATATGTCAAC

CRISPR arrays and Neighbor proteins around CP020114_12

CRISPR arrays

The CRISPR arrays of CP020114_12

>merge|CP020114|12|4035453-4037321|CRISPRCasFinder,CRT,PILER-CR
CTTTTTTGCCCCTTGCGGGGAAATGGTTATGAAAAACCTCAAGAAAGCTTCACAGGGAAGTTTAGGTGTATTCGTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAACTAAACGCACCAAAAAACGGAGGTTGACGAAAGTGGACGAGTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAACGCGATCGCCCCAAAGCAAAGCCGGATTCAACAATCTAGTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAACGAAGTTTAGTAGGGATGCTTAGAAGCCCTAATAGGAACGTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAACGTTGCTCAATTTATTAGAGAGTACAAATTAAAAGCCAAGTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAACTCATTATCCAAGATGGTGTGGGAGCATTTTAATGGCAAGGTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAACTAAACTCAGCCTTAAAAAGGTCAAACAGTTTTTTAATACCCTCCCAGTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAACAACTGAGGGGGTTAACCCCCTCAGTTTTCTCATACAATGTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAACAGCAAATCGCTGTAGCACAGCTTGACACGGTTAAACACACTAAGGAGTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAACTTCCGTTATGGGTTACGAACTGATTAAAGCCACGAAAGGGTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAACATCTAAAGTGGTCTGAGGGTAGTATTTATCCTCAACCGTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAACGATACAAGAGACACCCTTAATATCTTGTAATAACTGGGCATTGTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAACAGAAAAAACAATTGGATTTAAATATCCAAAGACAATATGTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAACCTGATAACTGTTGGATAGCTGAGTGAGATCGCTCACAAGGTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAACCATTAGTTGAAGATGGTTCTGGACAAGGTTTAGGTCATCCTGGTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAACAATTAGGGTTTACACCTTTAAGAGAAAATGCTTTTATTTATGTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAACGCTTTATGCAAGCTGCTTTGTTAGCCAGAAAAGAAAACCAGTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAACATCACCTCATCTAGGATTACAGTTAAAGTGCCTTCTGATGTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAACAAAGGGATGGCGACTTCTTTCCAATTATGTCTGCGTAGCGTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAACGAATGTTACAGAAAGCTTTGAAGAGTTTGGTATAACTGGGTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAACCAAGCAAAATTAGAAACAGTATTGGAAGCTGATGGCGTGTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAACCATATTAAGCTTTCAAATTTTCCTCTATTTTGGCTACAACGTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAACCAAAGGTCAAGGACTTTTTGCAGGACGATATAAAATTGTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAACTAGGACTCCGCTTCTGTTTACAAGAAGATATGTCAACGTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC

>CP020114|12|12|4035453-4037321|CRISPRCasFinder
CTTTTTTGCCCCTTGCGGGGAAATGGTTATGAAAAAC	CTCAAGAAAGCTTCACAGGGAAGTTTAGGTGTATTC
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	TAAACGCACCAAAAAACGGAGGTTGACGAAAGTGGACGA
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	GCGATCGCCCCAAAGCAAAGCCGGATTCAACAATCTA
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	GAAGTTTAGTAGGGATGCTTAGAAGCCCTAATAGGAAC
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	GTTGCTCAATTTATTAGAGAGTACAAATTAAAAGCCAA
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	TCATTATCCAAGATGGTGTGGGAGCATTTTAATGGCAAG
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	TAAACTCAGCCTTAAAAAGGTCAAACAGTTTTTTAATACCCTCCCA
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	AACTGAGGGGGTTAACCCCCTCAGTTTTCTCATACAAT
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	AGCAAATCGCTGTAGCACAGCTTGACACGGTTAAACACACTAAGGA
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	TTCCGTTATGGGTTACGAACTGATTAAAGCCACGAAAGG
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	ATCTAAAGTGGTCTGAGGGTAGTATTTATCCTCAACC
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	GATACAAGAGACACCCTTAATATCTTGTAATAACTGGGCATT
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	AGAAAAAACAATTGGATTTAAATATCCAAAGACAATAT
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	CTGATAACTGTTGGATAGCTGAGTGAGATCGCTCACAAG
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	CATTAGTTGAAGATGGTTCTGGACAAGGTTTAGGTCATCCTG
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	AATTAGGGTTTACACCTTTAAGAGAAAATGCTTTTATTTAT
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	GCTTTATGCAAGCTGCTTTGTTAGCCAGAAAAGAAAACCA
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	ATCACCTCATCTAGGATTACAGTTAAAGTGCCTTCTGAT
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	AAAGGGATGGCGACTTCTTTCCAATTATGTCTGCGTAGC
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	GAATGTTACAGAAAGCTTTGAAGAGTTTGGTATAACTGG
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	CAAGCAAAATTAGAAACAGTATTGGAAGCTGATGGCGT
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	CATATTAAGCTTTCAAATTTTCCTCTATTTTGGCTACAAC
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	CAAAGGTCAAGGACTTTTTGCAGGACGATATAAAATT
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	TAGGACTCCGCTTCTGTTTACAAGAAGATATGTCAAC
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC

>CP020114|12|2|4035453-4037321|CRT
CTTTTTTGCCCCTTGCGGGGAAATGGTTATGAAAAAC	CTCAAGAAAGCTTCACAGGGAAGTTTAGGTGTATTC
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	TAAACGCACCAAAAAACGGAGGTTGACGAAAGTGGACGA
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	GCGATCGCCCCAAAGCAAAGCCGGATTCAACAATCTA
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	GAAGTTTAGTAGGGATGCTTAGAAGCCCTAATAGGAAC
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	GTTGCTCAATTTATTAGAGAGTACAAATTAAAAGCCAA
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	TCATTATCCAAGATGGTGTGGGAGCATTTTAATGGCAAG
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	TAAACTCAGCCTTAAAAAGGTCAAACAGTTTTTTAATACCCTCCCA
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	AACTGAGGGGGTTAACCCCCTCAGTTTTCTCATACAAT
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	AGCAAATCGCTGTAGCACAGCTTGACACGGTTAAACACACTAAGGA
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	TTCCGTTATGGGTTACGAACTGATTAAAGCCACGAAAGG
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	ATCTAAAGTGGTCTGAGGGTAGTATTTATCCTCAACC
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	GATACAAGAGACACCCTTAATATCTTGTAATAACTGGGCATT
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	AGAAAAAACAATTGGATTTAAATATCCAAAGACAATAT
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	CTGATAACTGTTGGATAGCTGAGTGAGATCGCTCACAAG
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	CATTAGTTGAAGATGGTTCTGGACAAGGTTTAGGTCATCCTG
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	AATTAGGGTTTACACCTTTAAGAGAAAATGCTTTTATTTAT
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	GCTTTATGCAAGCTGCTTTGTTAGCCAGAAAAGAAAACCA
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	ATCACCTCATCTAGGATTACAGTTAAAGTGCCTTCTGAT
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	AAAGGGATGGCGACTTCTTTCCAATTATGTCTGCGTAGC
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	GAATGTTACAGAAAGCTTTGAAGAGTTTGGTATAACTGG
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	CAAGCAAAATTAGAAACAGTATTGGAAGCTGATGGCGT
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	CATATTAAGCTTTCAAATTTTCCTCTATTTTGGCTACAAC
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	CAAAGGTCAAGGACTTTTTGCAGGACGATATAAAATT
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	TAGGACTCCGCTTCTGTTTACAAGAAGATATGTCAAC
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC

>CP020114|12|2|4035526-4037321|PILER-CR
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	TAAACGCACCAAAAAACGGAGGTTGACGAAAGTGGACGA
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	GCGATCGCCCCAAAGCAAAGCCGGATTCAACAATCTA
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	GAAGTTTAGTAGGGATGCTTAGAAGCCCTAATAGGAAC
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	GTTGCTCAATTTATTAGAGAGTACAAATTAAAAGCCAA
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	TCATTATCCAAGATGGTGTGGGAGCATTTTAATGGCAAG
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	TAAACTCAGCCTTAAAAAGGTCAAACAGTTTTTTAATACCCTCCCA
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	AACTGAGGGGGTTAACCCCCTCAGTTTTCTCATACAAT
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	AGCAAATCGCTGTAGCACAGCTTGACACGGTTAAACACACTAAGGA
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	TTCCGTTATGGGTTACGAACTGATTAAAGCCACGAAAGG
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	ATCTAAAGTGGTCTGAGGGTAGTATTTATCCTCAACC
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	GATACAAGAGACACCCTTAATATCTTGTAATAACTGGGCATT
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	AGAAAAAACAATTGGATTTAAATATCCAAAGACAATAT
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	CTGATAACTGTTGGATAGCTGAGTGAGATCGCTCACAAG
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	CATTAGTTGAAGATGGTTCTGGACAAGGTTTAGGTCATCCTG
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	AATTAGGGTTTACACCTTTAAGAGAAAATGCTTTTATTTAT
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	GCTTTATGCAAGCTGCTTTGTTAGCCAGAAAAGAAAACCA
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	ATCACCTCATCTAGGATTACAGTTAAAGTGCCTTCTGAT
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	AAAGGGATGGCGACTTCTTTCCAATTATGTCTGCGTAGC
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	GAATGTTACAGAAAGCTTTGAAGAGTTTGGTATAACTGG
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	CAAGCAAAATTAGAAACAGTATTGGAAGCTGATGGCGT
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	CATATTAAGCTTTCAAATTTTCCTCTATTTTGGCTACAAC
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	CAAAGGTCAAGGACTTTTTGCAGGACGATATAAAATT
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC	TAGGACTCCGCTTCTGTTTACAAGAAGATATGTCAAC
GTTTCCGTCCCCTTGCGGGGAAATGGTTATGAAAAAC

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
CP020114.1|AVZ31155.1|4056955_4058038_-|CRISPR-associated-protein-Csm4	csm4gr5	cd09663_csm4gr5_CAS-III-A	gnl|CDD|187794
CP020114.1|AVZ31143.1|4027937_4029380_-|hypothetical-protein	WYL	mkCas0075_WYL_CAS-I:CAS-III	gnl|CDD|274901
CP020114.1|AVZ31140.1|4020684_4023228_-|hypothetical-protein	unknown	unknown	gnl|CDD|366045
CP020114.1|AVZ31151.1|4052817_4053420_-|hypothetical-protein	unknown	unknown	gnl|CDD|368559
CP020114.1|AVZ31153.1|4054921_4055680_-|CRISPR-associated-endoribonuclease-Cas6	cas6	pfam10040_cas6_CAS-III:CAS-IV	gnl|CDD|227838
CP020114.1|AVZ31157.1|4059014_4059566_-|CRISPR-associated-protein-Csm2	csm2gr11	pfam03750_csm2gr11_CAS-III-A	gnl|CDD|367635
CP020114.1|AVZ31152.1|4053748_4054882_-|hypothetical-protein	csm6	cd09742_csm6_CAS-III-A:CAS-III-D	gnl|CDD|187873
CP020114.1|AVZ31158.1|4059568_4061860_-|CRISPR-associated-protein-Csm1	cas10	cd09680_cas10_CAS-III	gnl|CDD|187811
CP020114.1|AVZ31149.1|4037653_4037938_-|CRISPR-associated-protein-Cas2	cas2	COG1343_cas2_CAS-I:CAS-II:CAS-III	gnl|CDD|224262
CP020114.1|AVZ31145.1|4031548_4033285_-|chaperone-protein-DnaK	unknown	unknown	gnl|CDD|212667
CP020114.1|AVZ31142.1|4026146_4027154_-|small-conductance-mechanosensitive-channel	unknown	unknown	gnl|CDD|223740
CP020114.1|AVZ31150.1|4037934_4038945_-|CRISPR-associated-endonuclease-Cas1	cas1	cd09634_cas1_CAS-I:CAS-II:CAS-III:CAS-V	gnl|CDD|272998
CP020114.1|AVZ31156.1|4058034_4059012_-|CRISPR-type-III-associated-RAMP-protein-Csm3	csm3gr7	COG1337_csm3gr7_CAS-III-D	gnl|CDD|274212
CP020114.1|AVZ31141.1|4024806_4026150_-|hypothetical-protein	unknown	unknown	gnl|CDD|378351
CP020114.1|AVZ31148.1|4035043_4035349_-|hypothetical-protein	unknown	unknown	unknown
CP020114.1|AVZ31154.1|4055685_4056972_-|CRISPR-associated-protein-Csm5	csm5gr7	COG1332_csm5gr7_CAS-III-A	gnl|CDD|187793
CP020114.1|AVZ31146.1|4033449_4034256_-|protein-GrpE	unknown	unknown	gnl|CDD|223649
CP020114.1|AVZ31147.1|4034663_4034987_-|hypothetical-protein	unknown	unknown	gnl|CDD|350220
CP020114.1|AVZ31139.1|4017138_4020543_-|hypothetical-protein	unknown	unknown	gnl|CDD|212667
CP020114.1|AVZ31144.1|4030032_4031535_-|chaperone-protein-DnaJ-1	unknown	unknown	gnl|CDD|365959

HMMScan for Signature genes

Protein	Cas_name	Cas_description	E-value	Identity	Coverage
CP020114.1|AVZ31143.1|4027937_4029380_-|hypothetical-protein	WYL	mkCas0075_WYL_CAS-I:CAS-III	2.8e-176	581.8	14.0
CP020114.1|AVZ31157.1|4059014_4059566_-|CRISPR-associated-protein-Csm2	csm2gr11	pfam03750_csm2gr11_CAS-III-A	2.8e-25	83.8	4.0
CP020114.1|AVZ31154.1|4055685_4056972_-|CRISPR-associated-protein-Csm5	csm5gr7	COG1332_csm5gr7_CAS-III-A	1e-23	79.3	0.2
CP020114.1|AVZ31153.1|4054921_4055680_-|CRISPR-associated-endoribonuclease-Cas6	cas6	pfam10040_cas6_CAS-III:CAS-IV	1.1e-24	82.0	0.0
CP020114.1|AVZ31152.1|4053748_4054882_-|hypothetical-protein	csm6	cd09742_csm6_CAS-III-A:CAS-III-D	2.8e-41	135.6	0.0
CP020114.1|AVZ31155.1|4056955_4058038_-|CRISPR-associated-protein-Csm4	csm4gr5	cd09663_csm4gr5_CAS-III-A	1.9e-46	153.4	0.0
CP020114.1|AVZ31158.1|4059568_4061860_-|CRISPR-associated-protein-Csm1	cas10	cd09680_cas10_CAS-III	1.9e-116	384.8	1.1
CP020114.1|AVZ31149.1|4037653_4037938_-|CRISPR-associated-protein-Cas2	cas2	COG1343_cas2_CAS-I:CAS-II:CAS-III	4.4e-29	95.4	0.0
CP020114.1|AVZ31150.1|4037934_4038945_-|CRISPR-associated-endonuclease-Cas1	cas1	cd09634_cas1_CAS-I:CAS-II:CAS-III:CAS-V	2.1e-92	304.3	0.0
CP020114.1|AVZ31156.1|4058034_4059012_-|CRISPR-type-III-associated-RAMP-protein-Csm3	csm3gr7	COG1337_csm3gr7_CAS-III-D	9.3e-71	232.9	0.0

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
CP020114.1|AVZ31155.1|4056955_4058038_-|CRISPR-associated-protein-Csm4	gnl|CDD|187794	cd09663, Csm4_III-A, CRISPR/Cas system-associated RAMP superfamily protein Csm4. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and associated Cas proteins comprise a system for heritable host defense by prokaryotic cells against phage and other foreign DNA; RAMP superfamily protein.	2.61111e-41
CP020114.1|AVZ31143.1|4027937_4029380_-|hypothetical-protein	gnl|CDD|274901	TIGR03985, hypothetical_protein_sll7078, CRISPR-associated protein, TIGR03985 family. Members of this protein family belong to CRISPR-associated (Cas) gene clusters. The majority of members are Cyanobacterial.	4.32615e-139
CP020114.1|AVZ31140.1|4020684_4023228_-|hypothetical-protein	gnl|CDD|366045	pfam00350, Dynamin_N, Dynamin family.	1.04406e-05
CP020114.1|AVZ31151.1|4052817_4053420_-|hypothetical-protein	gnl|CDD|368559	pfam05685, Uma2, Putative restriction endonuclease. This family consists of hypothetical proteins that are greatly expanded in cyanobacteria. The proteins are found sporadically in other bacteria. A small number of member proteins also contain pfam02861 domains that are involved in protein interactions. Solutions of several structures for members of this family show that it is likely to be acting as an endonuclease.	2.42858e-30
CP020114.1|AVZ31153.1|4054921_4055680_-|CRISPR-associated-endoribonuclease-Cas6	gnl|CDD|227838	COG5551, COG5551, CRISPR system related protein, RAMP superfamily [Defense mechanisms].	1.97885e-11
CP020114.1|AVZ31157.1|4059014_4059566_-|CRISPR-associated-protein-Csm2	gnl|CDD|367635	pfam03750, Csm2_III-A, Csm2 Type III-A. Clusters of short DNA repeats with non-homologous spacers, which are found at regular intervals in the genomes of phylogenetically distinct prokaryotic species, comprise a family with recognisable features. This family is known as CRISPR (short for Clustered Regularly Interspaced Short Palindromic Repeats). A number of protein families appear only in association with these repeats and are designated Cas (CRISPR-associated) proteins. This entry represents Csm2 Type III-A, a family of Cas proteins also known as TM1810/Csm2.	2.00124e-29
CP020114.1|AVZ31152.1|4053748_4054882_-|hypothetical-protein	gnl|CDD|187873	cd09742, Csm6_III-A, CRISPR/Cas system-associated protein Csm6. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and associated Cas proteins comprise a system for heritable host defense by prokaryotic cells against phage and other foreign DNA; Protein of this family often fused to HTH domain; loosely associated with CRISPR/Cas systems; also known as APE2256 family.	2.22759e-41
CP020114.1|AVZ31158.1|4059568_4061860_-|CRISPR-associated-protein-Csm1	gnl|CDD|187811	cd09680, Cas10_III, CRISPR/Cas system-associated protein Cas10. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and associated Cas proteins comprise a system for heritable host defense by prokaryotic cells against phage and other foreign DNA; Multidomain protein with permuted HD nuclease domain, palm domain and Zn-ribbon; signature gene for type III; also known as Csm1 family.	1.98395e-112
CP020114.1|AVZ31149.1|4037653_4037938_-|CRISPR-associated-protein-Cas2	gnl|CDD|224262	COG1343, COG1343, CRISPR-associated protein Cas2 [Defense mechanisms].	4.51424e-27
CP020114.1|AVZ31145.1|4031548_4033285_-|chaperone-protein-DnaK	gnl|CDD|212667	cd10170, HSP70_NBD, Nucleotide-binding domain of the HSP70 family. HSP70 (70-kDa heat shock protein) family chaperones assist in protein folding and assembly and can direct incompetent "client" proteins towards degradation. Typically, HSP70s have a nucleotide-binding domain (NBD) and a substrate-binding domain (SBD). The nucleotide sits in a deep cleft formed between the two lobes of the NBD. The two subdomains of each lobe change conformation between ATP-bound, ADP-bound, and nucleotide-free states. ATP binding opens up the substrate-binding site; substrate-binding increases the rate of ATP hydrolysis. HSP70 chaperone activity is regulated by various co-chaperones: J-domain proteins and nucleotide exchange factors (NEFs). Some HSP70 family members are not chaperones but instead, function as NEFs to remove ADP from their HSP70 chaperone partners during the ATP hydrolysis cycle, some may function as both chaperones and NEFs.	4.87394e-129
CP020114.1|AVZ31142.1|4026146_4027154_-|small-conductance-mechanosensitive-channel	gnl|CDD|223740	COG0668, MscS, Small-conductance mechanosensitive channel [Cell envelope biogenesis, outer membrane].	1.04447e-46
CP020114.1|AVZ31150.1|4037934_4038945_-|CRISPR-associated-endonuclease-Cas1	gnl|CDD|272998	TIGR00287, CRISPR-associated_endonuclease_Cas1, CRISPR-associated endonuclease Cas1. This model identifies CRISPR-associated protein Cas1, the most universal CRISPR system protein. CRISPR is an acronym for Clustered Regularly Interspaced Short Palindromic Repeats, a system for heritable host defense by prokaryotic cells against phage and other foreign DNA. Cas1 is a metal-dependent DNA-specific endonuclease.	2.99618e-110
CP020114.1|AVZ31156.1|4058034_4059012_-|CRISPR-type-III-associated-RAMP-protein-Csm3	gnl|CDD|274212	TIGR02582, CRISPR_type_III-associated_RAMP_protein_Csm3, CRISPR type III-A/MTUBE-associated RAMP protein Csm3. Members of this CRISPR-associated (cas) gene family are found in the mtube subtype of CRISPR/cas locus and designated Csm3, for CRISPR/cas Subtype Mtube, protein 3.	5.40805e-78
CP020114.1|AVZ31141.1|4024806_4026150_-|hypothetical-protein	gnl|CDD|378351	pfam10011, DUF2254, Predicted membrane protein (DUF2254). Members of this family of bacterial proteins comprises various hypothetical and putative membrane proteins. Their exact function, has not, as yet, been defined.	7.30249e-172
CP020114.1|AVZ31154.1|4055685_4056972_-|CRISPR-associated-protein-Csm5	gnl|CDD|187793	cd09662, Csm5_III-A, CRISPR/Cas system-associated RAMP superfamily protein Csm5. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and associated Cas proteins comprise a system for heritable host defense by prokaryotic cells against phage and other foreign DNA; RAMP superfamily protein.	1.93328e-12
CP020114.1|AVZ31146.1|4033449_4034256_-|protein-GrpE	gnl|CDD|223649	COG0576, GrpE, Molecular chaperone GrpE (heat shock protein) [Posttranslational modification, protein turnover, chaperones].	1.18555e-08
CP020114.1|AVZ31147.1|4034663_4034987_-|hypothetical-protein	gnl|CDD|350220	cd09872, PIN_Sll0205-like, VapC-like PIN domain of Sll0205 protein and homologs. Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of the Synechocystis sp. (strain PCC 6803) Sll0205 protein and other uncharacterized homologs are included in this subfamily. They are similar to the PIN domains of the Mycobacterium tuberculosis VapC and Neisseria gonorrhoeae FitB toxins of the prokaryotic toxin/antitoxin operons, VapBC and FitAB, respectively, which are believed to be involved in growth inhibition by regulating translation. These toxins are nearly always co-expressed with an antitoxin, a cognate protein inhibitor, forming an inert protein complex. Disassociation of the protein complex activates the ribonuclease activity of the toxin by an, as yet undefined mechanism. The PIN domain belongs to a large nuclease superfamily. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.	2.19841e-45
CP020114.1|AVZ31139.1|4017138_4020543_-|hypothetical-protein	gnl|CDD|212667	cd10170, HSP70_NBD, Nucleotide-binding domain of the HSP70 family. HSP70 (70-kDa heat shock protein) family chaperones assist in protein folding and assembly and can direct incompetent "client" proteins towards degradation. Typically, HSP70s have a nucleotide-binding domain (NBD) and a substrate-binding domain (SBD). The nucleotide sits in a deep cleft formed between the two lobes of the NBD. The two subdomains of each lobe change conformation between ATP-bound, ADP-bound, and nucleotide-free states. ATP binding opens up the substrate-binding site; substrate-binding increases the rate of ATP hydrolysis. HSP70 chaperone activity is regulated by various co-chaperones: J-domain proteins and nucleotide exchange factors (NEFs). Some HSP70 family members are not chaperones but instead, function as NEFs to remove ADP from their HSP70 chaperone partners during the ATP hydrolysis cycle, some may function as both chaperones and NEFs.	7.69222e-09
CP020114.1|AVZ31144.1|4030032_4031535_-|chaperone-protein-DnaJ-1	gnl|CDD|365959	pfam00226, DnaJ, DnaJ domain. DnaJ domains (J-domains) are associated with hsp70 heat-shock system and it is thought that this domain mediates the interaction. DnaJ-domain is therefore part of a chaperone (protein folding) system. The T-antigens, although not in Prosite are confirmed as DnaJ containing domains from literature.	8.47234e-16

Protein sequences

>CP020114.1|AVZ31148.1|4035043_4035349_-|hypothetical-protein
MSEKTFDIAYLSENLLDLLSDIQNHVEITLTRQGVPWAKVLPLEPEESTVEKAGLNHGSMAMNSEEVLEPRVPGFWQGKVKISEDFDDTSEEVIAAFYGEE
>CP020114.1|AVZ31147.1|4034663_4034987_-|hypothetical-protein
MSNQVREVIINPKNLIFVSAISAWEISIKQSLGKLIAPSNLEEALRFSRLEVLPMTLTHGIKVADLPMHHKDPFDRMLIAQALVEGLTIITVDQKFKFYDVPLLIIA
>CP020114.1|AVZ31146.1|4033449_4034256_-|protein-GrpE
MRNDKHPIPSYVKKSWLLYSITAIILITSLVGLAVALLPNAIIEIQRDILFIISAIYLLASCSFFLWIYLRNQDQEFVGILPKAIDDLKRVGREYYNNTAAIQDNSASVEQLHNRIKQEIRSFVESNKSLENLLIEVRQEKGNLQRELENWQESTIEFFQLLERALEQQNPDVQESLQKNLKEFERIVNKRGFYRIAPTPQDKFDEIQHEWKGKESSSEVEPGNILKCERWGYKSGAKVLQLAEVILAIAPENSNASEASNSIEISQV
>CP020114.1|AVZ31145.1|4031548_4033285_-|chaperone-protein-DnaK
MKSNYAIGIDLGTSTSEICIYKNNEPLPIPDPITKIPIIPSIVAINERGKLLVGETARGIVDRKGFGVRESKRNMGTDKVFTLREQDYRPEEIAALILRKLKENAEEALGHPIQDVVISVPANFPDAARKATFNAGEIAGLNILRLLNEPTAAALAFGIKNIASEEQLVVFDFGGGTLDISVLEMFEGVLDVKSSFGDPQLGGKDFDAVMISLLLQKFAAQYPEVAVENRETELKGQAEQAKKTLSIEQSCDVRIPYFATKDGKGIDLDVEITRTEFEQAIEPLLERARICIREALNAKKIRPSAIDRVLLVGGTTYIPAVRNMVAEMFGKQPKLDVNPDLAVGIGACIQAALAQGLINEENGIILTDVAPFGLGIDVVSMIGGQVMLTYEPLIERNTTIPYSVKKTYTLLHAEQQQVEVRLYQDHTGKARLPGDTQYTGISGEITDIPPAPNGNPYPIEIEFSYDINGMAKLTASIPDIRRSIEFAFGHSNNRMSQQETRVSTERIESLWKRHERAKQYESLINKAERFMAGIPPQERSPLSRAVMDLKEALTTDNSTNIDAAGDRLIDMMFAWENQ
>CP020114.1|AVZ31144.1|4030032_4031535_-|chaperone-protein-DnaJ-1
MAYELYQTLNIPTQASPEDIRRAYYRLVRKHSPEKDPERFKVIREAYETLSDAKAKQNYDSVQQHGGQIVVLLSQAEERMEEENWPEAIRLLKHTLVLSPGADSIRNRLGLCYIHHQQWDEATKTYQILTASNPDVAVYWSNFGCANKEIAETISEGDSRRSIYYQKARICFQKSVDLEPFNSQPFMAIAQTFLAEENYTQALKWAEKAIGADGKIDFQDFDALFLICIINLRSNQLEQVTATADRIIACLPADEDIRKYVAAKFALIGFELLEAGAKYINPQILKAALTFMSAAKRFSPDNAEIATIQREISEVVNAFNEYDILKDDYQIINGFKRLAAFRILNALGSEDSASERDRIFKDIISEISNSTDSEVLASAKRIKSKYPAIYRLNAEFFARLEKVAAEISRKQQYQPQTQPTNNSSDDCFVVTATYGTPHAAEVIKYRYFRDKYLRKHILGRKFIQHYYHIGPLAASQIKKHPNLKKIMALILGKLAKYLPN
>CP020114.1|AVZ31143.1|4027937_4029380_-|hypothetical-protein
MLQAIRSPTPQILHWLAAGQLANRFHRAIRHWWILNQLYGSDGQWGHYLPKYFTYSQLRDRLFAETHPKSEKLNPPEITAKCRDLTCICHRTCVEILQTSGLHISENQWQTEILQLSGINLQHLQEQLQQRPFATVHRSLRDDLKQLTHLGWLRKVNQGQYQCLPQTELPTPPEIAIAQPKFIQLSIPQTWELLHVLESVAFIQPNLNLIVENLWEQITHNSTSPLTQAPQQRIFLHLDYILSTETQDQVDNYQEQIEQLWRKTRGGVVRFDYWVTAENKVQVTVYPVCLHYLRRAKYLSAYGLDPHNNIAWHNYRLDRIASQKLQVLAWGDPAVPKPLKQMWYTGELPTPDYIQTELDAAWGFNFYLQREFLILRFPPAFASRYVDNTFRHPSFCAIPYENLPKQIVQNIPEAQQQKVLEILQQKPDSDAYYMGWIRTGDINVLMRLRDWRPNGEVIAPLSIRQQLRKEAIQELSNYQD
>CP020114.1|AVZ31142.1|4026146_4027154_-|small-conductance-mechanosensitive-channel
MNAEISALLDRVQKMANDFIILLPNIVLGLFVFTIFVIFGRSIKRLVKRLTRHRTYARNLGMVLGRLAQGTTVLVGLFISLTIVFPSLKASDLVQLLGISGVAIGFAFRDILQNFLAGILILLTEPFQINDQIVFKNFEGTVENIETRATTIRTYDGRRIVIPNSELFTNSVTVNTAFENRRLEYDVGVGYGDDLDWTKQLMLEAMHSLDVVLKDPPPDVLVMELAENSVNIRARWWIQPPRWSDALDSRDQVISAIKQKLYVENGIDLPYPTRQILFHDQTEETDGDRTRQREGWPSGKNKVPKPRSIGGSLQRLAQLQDRNGKVDAHERNHES
>CP020114.1|AVZ31141.1|4024806_4026150_-|hypothetical-protein
MNNVKLSKLWDALHTSYWFLPGILALSAVILAFTMLSLDRTIGFDDWDWIYTGGPDGAREVLSAIAGSMVSVAATAFSITIVALQLASANFGPRLLRNFMRDTGNQIVLGTFIATFIYSLLVLRAIYGMGYNLFIPHLSVTVGIVLAILSIAVLIYFIHHASTIIQASQVIKSVSEDLDKAIDRLFPEKIGVNPPTNQLHLGEIPPDFELQAYPIKANSNGYLQAINDEKIMEIACKYNLLIHVKSRPGKFVIKGSELVMVWPGERVNRKLNHRLQKTFILGRERTEHQDVEFPLQQLVEIALRAISPGINDPFTAIRCIDRLSAGLCHLVQRQFPSPYRYDDHNQLRVIAKPVTFEGIVAQAFNQIRQNSRTDVAVTIHLLKAIALIANCTHNGQYQQILKRHADMIQRGSHEGLPESQDRRDVQEKYNTVIQVLEQNHEIEILQR
>CP020114.1|AVZ31140.1|4020684_4023228_-|hypothetical-protein
MEIEQLDCFKEYGEFILQKLDSVPQYPSKQEDWIPTSLDDCLMRLREAAEKTVELATSPVRIGVMGEFSSGKTLLLGSLIGYADALPISENPTTGNVTAIHIVPQSGFVTTQANNFTVEYLSHEGVDECLQFMLHEASRRTTAAGLAPMPLSKLNTGKDIINWCEDTWNSSKNLELRYLLRELVLFIRAYQAYGDAMCGGHYQIDAITAREGLQLTEQPMAIQTLRFEDLPPSHIRLPSPPQRLPTKLLQNSFPLIRRVDMEVKISREIWDFAGASEFILLDFPGLGAANSGARDTFLSLRELAEVQTILVLLNGKTPGSDRANKIFTMMQQQRPGQDLKDLILVGVGRFDQLPLDSEGCERELDRFIESSRILEEADVFRKLKVLQTTIDAARAFTTQADRIVLLSPLLGLAELAKRSTTVKAGSPEFLANLDYPDYLERSKRLQQKWELLSTRLLESDAHSHLGKQLSYFAQDGGIAKLRELIQNHVTSHGVKQLHQDTRRAADNLLQHQEHLKNIITEIHEQGIPTADTQDFIEFRAAIENLDKTYRNFQKGLGKEPLKDRRGDVVSDVVKDELTFKILNWNEWTLLFNKANNGNITIAEFKGAAGKLFDRGNRVNSTLPTKSEDFYPAFAKTVKELENFARDRIHQAVADLLSQLSHEIAAERDHLQKTLHPEMEEDIETKFGVEEADIFYKLMLGCDPNKWQEAIISEINNKEQSISPEIMFPLARKDEKHNIGQIFDWSPEKSQDLPRTGNHQLFVLRLRDEIIACASLHLVQYVSEINQQVNAELEGILDQIIPTLQSLSKKEALLRHIAAGDSPAKVAIPTWLQVLSQVATSSYAEDHF
>CP020114.1|AVZ31139.1|4017138_4020543_-|hypothetical-protein
MAVEIRLVPNFQVRAKENEYLELPTIQLVAAGDNVPHIARINCIVTGTPEELAVQIKKAYKPFDSVTPKISQIGQLEQYPCKLERPLIEPINCTLEVIVEYFDSDTLGDPLLSEPRQTGASCYLWSPIIFEEEIIDSEPVSSPIEISKYIEKKIIQKPQKRFPGWFALDFGTSNSTVTLFDPIEVPIAEILPKEQEIRLRDRMVEWLSSPASVALPDVNPHDWDKFIADISKNLEIEPHRLREVFESDQKERFLEAIRQVELCLGNSDKFRRAASKKLYQIYHEVFRVPTLESQNLIPVVLDINRRDTEIPSELEISSLTDLQLRMGREARDNRKKAIAQGTSVSLKEIISRFHHSPKRYFGQDRTFPVILDDQEETINVNELIQAAWAHLIDLTEDYRQRARRRFSEGDFLTAVVTYPTVAPPLVRKEVKELVEQLGIDDVQTAYDEAVSVAIFFLWREFGGNLNIGIESFKTRCRQIENKWSQNVLVLDIGGGTTDLALIELTLEDKTPNFAEHEDRGLGGRYYKLTPKLLGSSGHLQLGGELITLRIFRLLKVAIADFLLTAITTGDIESEKLEDLINTELNERFLEQGKFQSGSLLKCLDKENPDGDAAYKDALDTAEKVLPTRWQQAPQRLQTFYTLWDYAEAAKLKLGQKPPADHPLLTFLISEAQISELLTQSAIKFQIRNPDNVCVIIDSQQFERAASSTIKEGIGIAKGLMESRLGFQDQNIDTWNTHKVDWLILSGKTCNLDLVQRHIYQEFSNSPYFVWNPERITFVLEFTKLATSAGACYAEKLRRLRFDPEESKELLRKGANQLEIDVKNLFYYLPCNFKRKTQSNELLPIFKAGQELYQITPSETAAKVRTEWQGIQLTNIIYRQDYEEGDLRLWGSFDGKNLMKKLGMQEAEFLKKIKVQFEIDQTLQFNVLLCQGNPHYLIDVPGIDVGAALLAASETSALFADGKLKWNIAVAGPKHNLNDGDIAVNVIESATVDQPNAYHLVFEVDKHDSKFLREFHYLHNSEQQSQNGLVSNPLPPFPQTSQHTFYIYQTDTTTNSKKWIRIGALSKPDIATDYPCKYCVTLDQKGILRMHAGEVPYWTSTSQECLKQAGCVYRAELELQPNEVDKERDPFCGIH
>CP020114.1|AVZ31149.1|4037653_4037938_-|CRISPR-associated-protein-Cas2
MTKLWLVCYDVADDKRRTKLAKLIEQRCQRVQYSVFECPLDEKALTQQLENRWLKVIKVSEDNLRIYPLDSTAKQQTRVYGSSPPYEPPDYLIL
>CP020114.1|AVZ31150.1|4037934_4038945_-|CRISPR-associated-endonuclease-Cas1
MTTLYLTEPGTVIRYRNESLTITKQEKSQNCRIAEITLVVVLPGVQLTDVVIAQLLDKGIETIFLRQDGQFRGRLQGQFATNPIIRLAQYRTVETTFGMAIAQKLVIGKIRNQRVLLQRRNRATKGEISQLAEAIDLISSYSSRLSDFTTPLNRDELMGVEGICARTYYQGLKHWFPPQWNFSGRNRRPPLDPINALLSWGYGVLLARVFSACVQAGLDPYLGFFHAIEPYRPNLVLDLMEEFRPIIVDQSVISIIQSDLLQSEDFQPSPDGEGIWLGTLAKKLFLGELERRFHTPILYPAQNRRLSLSQIVLEQARSLGRCLVNSRLEYEAFVIK
>CP020114.1|AVZ31151.1|4052817_4053420_-|hypothetical-protein
MTITKNRLTFDQFLNYDFPEEGRYELVNGEIMRIQATRKHDNLADFLTRKFDIEVESRNLNYRVSGRIAIRTLTASGKEQGRVPDVSVVDKNLWDANLSAYTAFIEPLQLAVEIVSTNWEDDYIDKLDEYQRLGISEYWIVDYLAIGSRNYLGNPKEPTIFVYTLNEQGIYQSMAYRGTDKIISPTFAELDLTVEQILGA
>CP020114.1|AVZ31152.1|4053748_4054882_-|hypothetical-protein
MVKTVICSVGTSAARNLGVKPADLANWVKQQKINDKISIEYAAETVFNTFSHVSPAGENLKNKLSAEIHSLFRIRITNQDKVILLASSTDDGYCCALAVEKYLKHYLDKIDVKTKKIPGLQVLDAELFRKEGVVNFVKCILKEIDGYDPSNFILNPTGGYKALVPYTVLLGMLKGIKCDYIFEQSSTLLELPPLPVEFKRSQFDTYQDLFEKIEHETEISQEEWDQRVPYKEREMLEPLVEFSNGHVTISAVGFLFLEEMRKPSVLVPFLSQKAIRDCFDNLAQLDNRDPFDFLLRAASSKDGFSQYEHINVGNGLRWLKPGNTTDRYLVSVEGWRMLVWRAIREDQEGPNYASKVKVNPDNDRRAYSPFMRMDFVN
>CP020114.1|AVZ31153.1|4054921_4055680_-|CRISPR-associated-endoribonuclease-Cas6
MLIHSTWTLSVSTSTVLPRSYGLELVKQLHYQLGLEMGSETIPSVSYSGIIGFASTSRDFITFHSEESYQLSLCGLNEVSAKAISHLNLGDSLEFLGAKFNIINREDEITSYEELYTNLVGNEPEPVRRFDLQFITPTAFAQGGVNLPLPLPTLMFRSWLERWNNFAPIYLGSDDLIAYLGEAIFIKNHKIQTRSYQLNKGFLNGFVGEVTLQVLNRADPLLANVANLLVQYARFSSTGMKTRLGMGQTKSI
>CP020114.1|AVZ31154.1|4055685_4056972_-|CRISPR-associated-protein-Csm5
MVVASESALKKPDFYESKRIQLTSPLLHIGSSVSKLNPFEYVQIAKKVYLPNQEALAKGLLKKGGSFLNDYIQVIEERQDITRLLKQAFGDEWWNAKDTDENPIFPKDAISQNFTQERITDLRPMIRNGMGYLFIPGSSIKGAIRTAIAYHLLKYPDRYQVPLEYRSSQIEDRLKNSLGELRNKHKQKFADDKLFMDNFFSNYTLNYQGKKLSGSSQNTDFLRCLKVSDSEPIIERKVPSKTGKSIPVNFSVVGEVIVSSRYPDYLAKYKASIYAEMARNVNTEFTLTLDTEMLSWFEHKQGMKLPFKNLNELLQICEDFAQEQWDYEHDYWNTIENNPRASGKNLDFNHIRDLYEPEKCPYTLRLGWGSGMTGTTVGLCFDDEMRAEIRDICGIKAPGFEAPKSRRTVMNSNGEIKFVPGWVKFRAL
>CP020114.1|AVZ31155.1|4056955_4058038_-|CRISPR-associated-protein-Csm4
MSVWKLVELNFERNLAHFGEVGIGMETTGDRIRSDSLFSAWVSNYARLFQKEGVEELLQLFPTDKQPQLIPPFRISSTFIYRQVGVNIIYYLPRPLKFPINYPDEDLAFFKAYKKLNYLPLEIWERWYQGTGFTTDDAEELEKHISDKTQGKLLATAETFNHSKACKIQQLPKIAIDRNTRATNIYHTGFVQFAWEKDKNPAGLYFLLELSPEGEKLADKLQAALHLLGEEGIGGERSSGAGRFKVSWLELPKNWQKVVNFQGGNHHTLMSLFWDDDISSDLLKNSSYEILERGGWIAESQLRRQMVRMFSEGSVFSTLPQGKLVDVKPKEFTKHSIYRSGISLSLPIKVQEKENHGCCV
>CP020114.1|AVZ31156.1|4058034_4059012_-|CRISPR-type-III-associated-RAMP-protein-Csm3
MPVSYEQTPLVGKVTLTCQLTAKTGLHIGGGGENLDIGGLDKPVIRDPLTKYPYLPGSSIKGKLRATLERLLKKPLNRTGGSGTWRYESDDLVDGFSEVETETFIRYEGAITCEISRLFGSTGGSNFWIPIETARNEGLIFDEIHTRTIENQNCVKINRGRNAPSRLIIRDCHLSPDSAEKLKKVDTGLYMTEWKFENGIDRVTAAANPRQLERVPAESKFDFELIYTVENQEQAVEDLQNIAIALAFLEDDALGGHGSRGYGKVKFENFRFNYRSLEQYRQIANATQESSALQTLDAIPNTQALLDNFRSLREYIQRLLPGNES
>CP020114.1|AVZ31157.1|4059014_4059566_-|CRISPR-associated-protein-Csm2
MNKPNKLQPPNTRNSGKIVSQSTNPSQSYSRVNRETNTNIVKEIKEEIGKSDYLKDYPIRILVQQSEDLGYYLKEQRLETNQIRKFLDAINQIKYILALDDDEKIKNAKTEDQEKLEFSKIETEIVLLKPKLAYAAARQNAVKPLNEVMKFAIDRVKTPQDFYRLVQFIESIIAYHKEADSRR
>CP020114.1|AVZ31158.1|4059568_4061860_-|CRISPR-associated-protein-Csm1
MVTSSEKVALQVIQQAISALAKWANFAHPPLDNLDKNEFPEVTRAKEILDWKPETKIGILSLVFDSVNLSDKPQKQTTKHYHPLKAIDNDDKEYPDIPYPLNSAPSSEQQQAFKTEINKALSKYLKDNWENLSLLMLILEKFGSCLSFGEADVALVDIARTTAGVAVALLNNPTEKISLIAGDLSGIQKFIYSISSEGALKSLRARSFYLELVTEEVVQQLLEQLNLPRTNIIYAGGGNLYILASGTADNQNIVQNIRQQFNEWLLAKFEGKVFLALDCLEFTVTELKDNFAKHWVKATKNLAVHKSRKFAENQISKFIAPRYSHEPCRVCHRDDVETLEPLNSEEPDSVLACGTCRSMFDLGSQLLDVKAIVRSTNESVRKGLPTLSFKINSREIHYHLFKNLEEINTDSDRVLLVNDWQLEHYEFKHFKNAAPLLLGNYGKKSEQKPENTEEPIGFMRAGEMSEKAKGANRVGYLRMDVDRLGQIFAKGLGKKQTLSRLAGLSRQMSYFFKVYLNTLAAYINLKNPDIIKPVKPDEQGLNILFIYAGGDDLFISGAWSEIVEFAFEIYRSFRTYTGNNEHITLSGGISIDDIKFPLYQAAKSSGKAEEAAKGNGRDSLGLFGEVFKWDEWEGRQDFKHLDDDVKKYLDSETLPSLFGVYPFVKRLEQQDIGVNYSRNFVRNLLITAEIQEQALKKFKENEKSQEALGTRYYLHLPKIAYTLARLPKKVLEDIDFRTSLKNPYNAPYFRAIATWIELLNRGN

Crispr_ID: CP020114_13

• CRISPR_ID: CP020114_13
• CRISPR_location: 4039199-4039298
• CRISPR_type: TypeIII-A
• Repeat_type: NA
• Spacer_info: 1 spacers
• Cas_protein_info: cas1,cas2,WYL,csm6,cas6,csm5gr7,csm4gr5,csm3gr7,csm2gr11,cas10,csx3

Consensus repeat of CP020114_13

Consensus_repeat	Method
CCCAGCCCCTCTCCTTGGTAAGGAGAG	CRISPRCasFinder

spacers of CP020114_13

>13.1|4039226|46|CP020114|CRISPRCasFinder
CTAATAACTGATTTTGTCCTTTTACAACACTTCTAAGCCTTAGCGC

CRISPR arrays and Neighbor proteins around CP020114_13

CRISPR arrays

The CRISPR arrays of CP020114_13

>merge|CP020114|13|4039199-4039298|CRISPRCasFinder
CCCAGCCCCTCTCCTTGGTAAGGAGAGCTAATAACTGATTTTGTCCTTTTACAACACTTCTAAGCCTTAGCGCCCCAGCCCCTCTCCTTGGTAAGGAGAG

>CP020114|13|13|4039199-4039298|CRISPRCasFinder
CCCAGCCCCTCTCCTTGGTAAGGAGAG	CTAATAACTGATTTTGTCCTTTTACAACACTTCTAAGCCTTAGCGC
CCCAGCCCCTCTCCTTGGTAAGGAGAG

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
CP020114.1|AVZ31143.1|4027937_4029380_-|hypothetical-protein	WYL	mkCas0075_WYL_CAS-I:CAS-III	gnl|CDD|274901
CP020114.1|AVZ31157.1|4059014_4059566_-|CRISPR-associated-protein-Csm2	csm2gr11	pfam03750_csm2gr11_CAS-III-A	gnl|CDD|367635
CP020114.1|AVZ31151.1|4052817_4053420_-|hypothetical-protein	unknown	unknown	gnl|CDD|368559
CP020114.1|AVZ31153.1|4054921_4055680_-|CRISPR-associated-endoribonuclease-Cas6	cas6	pfam10040_cas6_CAS-III:CAS-IV	gnl|CDD|227838
CP020114.1|AVZ31159.1|4062125_4062431_-|CRISPR-associated-protein-Csx3	csx3	mkCas0115_csx3_CAS-I:CAS-III	gnl|CDD|187871
CP020114.1|AVZ31152.1|4053748_4054882_-|hypothetical-protein	csm6	cd09742_csm6_CAS-III-A:CAS-III-D	gnl|CDD|187873
CP020114.1|AVZ31158.1|4059568_4061860_-|CRISPR-associated-protein-Csm1	cas10	cd09680_cas10_CAS-III	gnl|CDD|187811
CP020114.1|AVZ31149.1|4037653_4037938_-|CRISPR-associated-protein-Cas2	cas2	COG1343_cas2_CAS-I:CAS-II:CAS-III	gnl|CDD|224262
CP020114.1|AVZ31145.1|4031548_4033285_-|chaperone-protein-DnaK	unknown	unknown	gnl|CDD|212667
CP020114.1|AVZ31142.1|4026146_4027154_-|small-conductance-mechanosensitive-channel	unknown	unknown	gnl|CDD|223740
CP020114.1|AVZ31150.1|4037934_4038945_-|CRISPR-associated-endonuclease-Cas1	cas1	cd09634_cas1_CAS-I:CAS-II:CAS-III:CAS-V	gnl|CDD|272998
CP020114.1|AVZ31156.1|4058034_4059012_-|CRISPR-type-III-associated-RAMP-protein-Csm3	csm3gr7	COG1337_csm3gr7_CAS-III-D	gnl|CDD|274212
CP020114.1|AVZ31141.1|4024806_4026150_-|hypothetical-protein	unknown	unknown	gnl|CDD|378351
CP020114.1|AVZ31148.1|4035043_4035349_-|hypothetical-protein	unknown	unknown	unknown
CP020114.1|AVZ31154.1|4055685_4056972_-|CRISPR-associated-protein-Csm5	csm5gr7	COG1332_csm5gr7_CAS-III-A	gnl|CDD|187793
CP020114.1|AVZ31160.1|4066573_4066921_-|hypothetical-protein	unknown	unknown	gnl|CDD|225296
CP020114.1|AVZ31146.1|4033449_4034256_-|protein-GrpE	unknown	unknown	gnl|CDD|223649
CP020114.1|AVZ31147.1|4034663_4034987_-|hypothetical-protein	unknown	unknown	gnl|CDD|350220
CP020114.1|AVZ31155.1|4056955_4058038_-|CRISPR-associated-protein-Csm4	csm4gr5	cd09663_csm4gr5_CAS-III-A	gnl|CDD|187794
CP020114.1|AVZ31144.1|4030032_4031535_-|chaperone-protein-DnaJ-1	unknown	unknown	gnl|CDD|365959

HMMScan for Signature genes

Protein	Cas_name	Cas_description	E-value	Identity	Coverage
CP020114.1|AVZ31143.1|4027937_4029380_-|hypothetical-protein	WYL	mkCas0075_WYL_CAS-I:CAS-III	2.8e-176	581.8	14.0
CP020114.1|AVZ31157.1|4059014_4059566_-|CRISPR-associated-protein-Csm2	csm2gr11	pfam03750_csm2gr11_CAS-III-A	2.8e-25	83.8	4.0
CP020114.1|AVZ31154.1|4055685_4056972_-|CRISPR-associated-protein-Csm5	csm5gr7	COG1332_csm5gr7_CAS-III-A	1e-23	79.3	0.2
CP020114.1|AVZ31153.1|4054921_4055680_-|CRISPR-associated-endoribonuclease-Cas6	cas6	pfam10040_cas6_CAS-III:CAS-IV	1.1e-24	82.0	0.0
CP020114.1|AVZ31159.1|4062125_4062431_-|CRISPR-associated-protein-Csx3	csx3	mkCas0115_csx3_CAS-I:CAS-III	6.3e-20	65.9	0.0
CP020114.1|AVZ31152.1|4053748_4054882_-|hypothetical-protein	csm6	cd09742_csm6_CAS-III-A:CAS-III-D	2.8e-41	135.6	0.0
CP020114.1|AVZ31155.1|4056955_4058038_-|CRISPR-associated-protein-Csm4	csm4gr5	cd09663_csm4gr5_CAS-III-A	1.9e-46	153.4	0.0
CP020114.1|AVZ31158.1|4059568_4061860_-|CRISPR-associated-protein-Csm1	cas10	cd09680_cas10_CAS-III	1.9e-116	384.8	1.1
CP020114.1|AVZ31149.1|4037653_4037938_-|CRISPR-associated-protein-Cas2	cas2	COG1343_cas2_CAS-I:CAS-II:CAS-III	4.4e-29	95.4	0.0
CP020114.1|AVZ31150.1|4037934_4038945_-|CRISPR-associated-endonuclease-Cas1	cas1	cd09634_cas1_CAS-I:CAS-II:CAS-III:CAS-V	2.1e-92	304.3	0.0
CP020114.1|AVZ31156.1|4058034_4059012_-|CRISPR-type-III-associated-RAMP-protein-Csm3	csm3gr7	COG1337_csm3gr7_CAS-III-D	9.3e-71	232.9	0.0

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
CP020114.1|AVZ31143.1|4027937_4029380_-|hypothetical-protein	gnl|CDD|274901	TIGR03985, hypothetical_protein_sll7078, CRISPR-associated protein, TIGR03985 family. Members of this protein family belong to CRISPR-associated (Cas) gene clusters. The majority of members are Cyanobacterial.	4.32615e-139
CP020114.1|AVZ31157.1|4059014_4059566_-|CRISPR-associated-protein-Csm2	gnl|CDD|367635	pfam03750, Csm2_III-A, Csm2 Type III-A. Clusters of short DNA repeats with non-homologous spacers, which are found at regular intervals in the genomes of phylogenetically distinct prokaryotic species, comprise a family with recognisable features. This family is known as CRISPR (short for Clustered Regularly Interspaced Short Palindromic Repeats). A number of protein families appear only in association with these repeats and are designated Cas (CRISPR-associated) proteins. This entry represents Csm2 Type III-A, a family of Cas proteins also known as TM1810/Csm2.	2.00124e-29
CP020114.1|AVZ31151.1|4052817_4053420_-|hypothetical-protein	gnl|CDD|368559	pfam05685, Uma2, Putative restriction endonuclease. This family consists of hypothetical proteins that are greatly expanded in cyanobacteria. The proteins are found sporadically in other bacteria. A small number of member proteins also contain pfam02861 domains that are involved in protein interactions. Solutions of several structures for members of this family show that it is likely to be acting as an endonuclease.	2.42858e-30
CP020114.1|AVZ31153.1|4054921_4055680_-|CRISPR-associated-endoribonuclease-Cas6	gnl|CDD|227838	COG5551, COG5551, CRISPR system related protein, RAMP superfamily [Defense mechanisms].	1.97885e-11
CP020114.1|AVZ31159.1|4062125_4062431_-|CRISPR-associated-protein-Csx3	gnl|CDD|187871	cd09740, Csx3_III-U, CRISPR/Cas system-associated protein Csx3. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and associated Cas proteins comprise a system for heritable host defense by prokaryotic cells against phage and other foreign DNA; Small protein in some cases fused to Csx1 (COG1517) family domains.	0.000119647
CP020114.1|AVZ31152.1|4053748_4054882_-|hypothetical-protein	gnl|CDD|187873	cd09742, Csm6_III-A, CRISPR/Cas system-associated protein Csm6. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and associated Cas proteins comprise a system for heritable host defense by prokaryotic cells against phage and other foreign DNA; Protein of this family often fused to HTH domain; loosely associated with CRISPR/Cas systems; also known as APE2256 family.	2.22759e-41
CP020114.1|AVZ31158.1|4059568_4061860_-|CRISPR-associated-protein-Csm1	gnl|CDD|187811	cd09680, Cas10_III, CRISPR/Cas system-associated protein Cas10. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and associated Cas proteins comprise a system for heritable host defense by prokaryotic cells against phage and other foreign DNA; Multidomain protein with permuted HD nuclease domain, palm domain and Zn-ribbon; signature gene for type III; also known as Csm1 family.	1.98395e-112
CP020114.1|AVZ31149.1|4037653_4037938_-|CRISPR-associated-protein-Cas2	gnl|CDD|224262	COG1343, COG1343, CRISPR-associated protein Cas2 [Defense mechanisms].	4.51424e-27
CP020114.1|AVZ31145.1|4031548_4033285_-|chaperone-protein-DnaK	gnl|CDD|212667	cd10170, HSP70_NBD, Nucleotide-binding domain of the HSP70 family. HSP70 (70-kDa heat shock protein) family chaperones assist in protein folding and assembly and can direct incompetent "client" proteins towards degradation. Typically, HSP70s have a nucleotide-binding domain (NBD) and a substrate-binding domain (SBD). The nucleotide sits in a deep cleft formed between the two lobes of the NBD. The two subdomains of each lobe change conformation between ATP-bound, ADP-bound, and nucleotide-free states. ATP binding opens up the substrate-binding site; substrate-binding increases the rate of ATP hydrolysis. HSP70 chaperone activity is regulated by various co-chaperones: J-domain proteins and nucleotide exchange factors (NEFs). Some HSP70 family members are not chaperones but instead, function as NEFs to remove ADP from their HSP70 chaperone partners during the ATP hydrolysis cycle, some may function as both chaperones and NEFs.	4.87394e-129
CP020114.1|AVZ31142.1|4026146_4027154_-|small-conductance-mechanosensitive-channel	gnl|CDD|223740	COG0668, MscS, Small-conductance mechanosensitive channel [Cell envelope biogenesis, outer membrane].	1.04447e-46
CP020114.1|AVZ31150.1|4037934_4038945_-|CRISPR-associated-endonuclease-Cas1	gnl|CDD|272998	TIGR00287, CRISPR-associated_endonuclease_Cas1, CRISPR-associated endonuclease Cas1. This model identifies CRISPR-associated protein Cas1, the most universal CRISPR system protein. CRISPR is an acronym for Clustered Regularly Interspaced Short Palindromic Repeats, a system for heritable host defense by prokaryotic cells against phage and other foreign DNA. Cas1 is a metal-dependent DNA-specific endonuclease.	2.99618e-110
CP020114.1|AVZ31156.1|4058034_4059012_-|CRISPR-type-III-associated-RAMP-protein-Csm3	gnl|CDD|274212	TIGR02582, CRISPR_type_III-associated_RAMP_protein_Csm3, CRISPR type III-A/MTUBE-associated RAMP protein Csm3. Members of this CRISPR-associated (cas) gene family are found in the mtube subtype of CRISPR/cas locus and designated Csm3, for CRISPR/cas Subtype Mtube, protein 3.	5.40805e-78
CP020114.1|AVZ31141.1|4024806_4026150_-|hypothetical-protein	gnl|CDD|378351	pfam10011, DUF2254, Predicted membrane protein (DUF2254). Members of this family of bacterial proteins comprises various hypothetical and putative membrane proteins. Their exact function, has not, as yet, been defined.	7.30249e-172
CP020114.1|AVZ31154.1|4055685_4056972_-|CRISPR-associated-protein-Csm5	gnl|CDD|187793	cd09662, Csm5_III-A, CRISPR/Cas system-associated RAMP superfamily protein Csm5. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and associated Cas proteins comprise a system for heritable host defense by prokaryotic cells against phage and other foreign DNA; RAMP superfamily protein.	1.93328e-12
CP020114.1|AVZ31160.1|4066573_4066921_-|hypothetical-protein	gnl|CDD|225296	COG2452, COG2452, Predicted site-specific integrase-resolvase [DNA replication, recombination, and repair].	1.19368e-18
CP020114.1|AVZ31146.1|4033449_4034256_-|protein-GrpE	gnl|CDD|223649	COG0576, GrpE, Molecular chaperone GrpE (heat shock protein) [Posttranslational modification, protein turnover, chaperones].	1.18555e-08
CP020114.1|AVZ31147.1|4034663_4034987_-|hypothetical-protein	gnl|CDD|350220	cd09872, PIN_Sll0205-like, VapC-like PIN domain of Sll0205 protein and homologs. Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of the Synechocystis sp. (strain PCC 6803) Sll0205 protein and other uncharacterized homologs are included in this subfamily. They are similar to the PIN domains of the Mycobacterium tuberculosis VapC and Neisseria gonorrhoeae FitB toxins of the prokaryotic toxin/antitoxin operons, VapBC and FitAB, respectively, which are believed to be involved in growth inhibition by regulating translation. These toxins are nearly always co-expressed with an antitoxin, a cognate protein inhibitor, forming an inert protein complex. Disassociation of the protein complex activates the ribonuclease activity of the toxin by an, as yet undefined mechanism. The PIN domain belongs to a large nuclease superfamily. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.	2.19841e-45
CP020114.1|AVZ31155.1|4056955_4058038_-|CRISPR-associated-protein-Csm4	gnl|CDD|187794	cd09663, Csm4_III-A, CRISPR/Cas system-associated RAMP superfamily protein Csm4. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and associated Cas proteins comprise a system for heritable host defense by prokaryotic cells against phage and other foreign DNA; RAMP superfamily protein.	2.61111e-41
CP020114.1|AVZ31144.1|4030032_4031535_-|chaperone-protein-DnaJ-1	gnl|CDD|365959	pfam00226, DnaJ, DnaJ domain. DnaJ domains (J-domains) are associated with hsp70 heat-shock system and it is thought that this domain mediates the interaction. DnaJ-domain is therefore part of a chaperone (protein folding) system. The T-antigens, although not in Prosite are confirmed as DnaJ containing domains from literature.	8.47234e-16

Protein sequences

>CP020114.1|AVZ31150.1|4037934_4038945_-|CRISPR-associated-endonuclease-Cas1
MTTLYLTEPGTVIRYRNESLTITKQEKSQNCRIAEITLVVVLPGVQLTDVVIAQLLDKGIETIFLRQDGQFRGRLQGQFATNPIIRLAQYRTVETTFGMAIAQKLVIGKIRNQRVLLQRRNRATKGEISQLAEAIDLISSYSSRLSDFTTPLNRDELMGVEGICARTYYQGLKHWFPPQWNFSGRNRRPPLDPINALLSWGYGVLLARVFSACVQAGLDPYLGFFHAIEPYRPNLVLDLMEEFRPIIVDQSVISIIQSDLLQSEDFQPSPDGEGIWLGTLAKKLFLGELERRFHTPILYPAQNRRLSLSQIVLEQARSLGRCLVNSRLEYEAFVIK
>CP020114.1|AVZ31149.1|4037653_4037938_-|CRISPR-associated-protein-Cas2
MTKLWLVCYDVADDKRRTKLAKLIEQRCQRVQYSVFECPLDEKALTQQLENRWLKVIKVSEDNLRIYPLDSTAKQQTRVYGSSPPYEPPDYLIL
>CP020114.1|AVZ31148.1|4035043_4035349_-|hypothetical-protein
MSEKTFDIAYLSENLLDLLSDIQNHVEITLTRQGVPWAKVLPLEPEESTVEKAGLNHGSMAMNSEEVLEPRVPGFWQGKVKISEDFDDTSEEVIAAFYGEE
>CP020114.1|AVZ31147.1|4034663_4034987_-|hypothetical-protein
MSNQVREVIINPKNLIFVSAISAWEISIKQSLGKLIAPSNLEEALRFSRLEVLPMTLTHGIKVADLPMHHKDPFDRMLIAQALVEGLTIITVDQKFKFYDVPLLIIA
>CP020114.1|AVZ31146.1|4033449_4034256_-|protein-GrpE
MRNDKHPIPSYVKKSWLLYSITAIILITSLVGLAVALLPNAIIEIQRDILFIISAIYLLASCSFFLWIYLRNQDQEFVGILPKAIDDLKRVGREYYNNTAAIQDNSASVEQLHNRIKQEIRSFVESNKSLENLLIEVRQEKGNLQRELENWQESTIEFFQLLERALEQQNPDVQESLQKNLKEFERIVNKRGFYRIAPTPQDKFDEIQHEWKGKESSSEVEPGNILKCERWGYKSGAKVLQLAEVILAIAPENSNASEASNSIEISQV
>CP020114.1|AVZ31145.1|4031548_4033285_-|chaperone-protein-DnaK
MKSNYAIGIDLGTSTSEICIYKNNEPLPIPDPITKIPIIPSIVAINERGKLLVGETARGIVDRKGFGVRESKRNMGTDKVFTLREQDYRPEEIAALILRKLKENAEEALGHPIQDVVISVPANFPDAARKATFNAGEIAGLNILRLLNEPTAAALAFGIKNIASEEQLVVFDFGGGTLDISVLEMFEGVLDVKSSFGDPQLGGKDFDAVMISLLLQKFAAQYPEVAVENRETELKGQAEQAKKTLSIEQSCDVRIPYFATKDGKGIDLDVEITRTEFEQAIEPLLERARICIREALNAKKIRPSAIDRVLLVGGTTYIPAVRNMVAEMFGKQPKLDVNPDLAVGIGACIQAALAQGLINEENGIILTDVAPFGLGIDVVSMIGGQVMLTYEPLIERNTTIPYSVKKTYTLLHAEQQQVEVRLYQDHTGKARLPGDTQYTGISGEITDIPPAPNGNPYPIEIEFSYDINGMAKLTASIPDIRRSIEFAFGHSNNRMSQQETRVSTERIESLWKRHERAKQYESLINKAERFMAGIPPQERSPLSRAVMDLKEALTTDNSTNIDAAGDRLIDMMFAWENQ
>CP020114.1|AVZ31144.1|4030032_4031535_-|chaperone-protein-DnaJ-1
MAYELYQTLNIPTQASPEDIRRAYYRLVRKHSPEKDPERFKVIREAYETLSDAKAKQNYDSVQQHGGQIVVLLSQAEERMEEENWPEAIRLLKHTLVLSPGADSIRNRLGLCYIHHQQWDEATKTYQILTASNPDVAVYWSNFGCANKEIAETISEGDSRRSIYYQKARICFQKSVDLEPFNSQPFMAIAQTFLAEENYTQALKWAEKAIGADGKIDFQDFDALFLICIINLRSNQLEQVTATADRIIACLPADEDIRKYVAAKFALIGFELLEAGAKYINPQILKAALTFMSAAKRFSPDNAEIATIQREISEVVNAFNEYDILKDDYQIINGFKRLAAFRILNALGSEDSASERDRIFKDIISEISNSTDSEVLASAKRIKSKYPAIYRLNAEFFARLEKVAAEISRKQQYQPQTQPTNNSSDDCFVVTATYGTPHAAEVIKYRYFRDKYLRKHILGRKFIQHYYHIGPLAASQIKKHPNLKKIMALILGKLAKYLPN
>CP020114.1|AVZ31143.1|4027937_4029380_-|hypothetical-protein
MLQAIRSPTPQILHWLAAGQLANRFHRAIRHWWILNQLYGSDGQWGHYLPKYFTYSQLRDRLFAETHPKSEKLNPPEITAKCRDLTCICHRTCVEILQTSGLHISENQWQTEILQLSGINLQHLQEQLQQRPFATVHRSLRDDLKQLTHLGWLRKVNQGQYQCLPQTELPTPPEIAIAQPKFIQLSIPQTWELLHVLESVAFIQPNLNLIVENLWEQITHNSTSPLTQAPQQRIFLHLDYILSTETQDQVDNYQEQIEQLWRKTRGGVVRFDYWVTAENKVQVTVYPVCLHYLRRAKYLSAYGLDPHNNIAWHNYRLDRIASQKLQVLAWGDPAVPKPLKQMWYTGELPTPDYIQTELDAAWGFNFYLQREFLILRFPPAFASRYVDNTFRHPSFCAIPYENLPKQIVQNIPEAQQQKVLEILQQKPDSDAYYMGWIRTGDINVLMRLRDWRPNGEVIAPLSIRQQLRKEAIQELSNYQD
>CP020114.1|AVZ31142.1|4026146_4027154_-|small-conductance-mechanosensitive-channel
MNAEISALLDRVQKMANDFIILLPNIVLGLFVFTIFVIFGRSIKRLVKRLTRHRTYARNLGMVLGRLAQGTTVLVGLFISLTIVFPSLKASDLVQLLGISGVAIGFAFRDILQNFLAGILILLTEPFQINDQIVFKNFEGTVENIETRATTIRTYDGRRIVIPNSELFTNSVTVNTAFENRRLEYDVGVGYGDDLDWTKQLMLEAMHSLDVVLKDPPPDVLVMELAENSVNIRARWWIQPPRWSDALDSRDQVISAIKQKLYVENGIDLPYPTRQILFHDQTEETDGDRTRQREGWPSGKNKVPKPRSIGGSLQRLAQLQDRNGKVDAHERNHES
>CP020114.1|AVZ31141.1|4024806_4026150_-|hypothetical-protein
MNNVKLSKLWDALHTSYWFLPGILALSAVILAFTMLSLDRTIGFDDWDWIYTGGPDGAREVLSAIAGSMVSVAATAFSITIVALQLASANFGPRLLRNFMRDTGNQIVLGTFIATFIYSLLVLRAIYGMGYNLFIPHLSVTVGIVLAILSIAVLIYFIHHASTIIQASQVIKSVSEDLDKAIDRLFPEKIGVNPPTNQLHLGEIPPDFELQAYPIKANSNGYLQAINDEKIMEIACKYNLLIHVKSRPGKFVIKGSELVMVWPGERVNRKLNHRLQKTFILGRERTEHQDVEFPLQQLVEIALRAISPGINDPFTAIRCIDRLSAGLCHLVQRQFPSPYRYDDHNQLRVIAKPVTFEGIVAQAFNQIRQNSRTDVAVTIHLLKAIALIANCTHNGQYQQILKRHADMIQRGSHEGLPESQDRRDVQEKYNTVIQVLEQNHEIEILQR
>CP020114.1|AVZ31151.1|4052817_4053420_-|hypothetical-protein
MTITKNRLTFDQFLNYDFPEEGRYELVNGEIMRIQATRKHDNLADFLTRKFDIEVESRNLNYRVSGRIAIRTLTASGKEQGRVPDVSVVDKNLWDANLSAYTAFIEPLQLAVEIVSTNWEDDYIDKLDEYQRLGISEYWIVDYLAIGSRNYLGNPKEPTIFVYTLNEQGIYQSMAYRGTDKIISPTFAELDLTVEQILGA
>CP020114.1|AVZ31152.1|4053748_4054882_-|hypothetical-protein
MVKTVICSVGTSAARNLGVKPADLANWVKQQKINDKISIEYAAETVFNTFSHVSPAGENLKNKLSAEIHSLFRIRITNQDKVILLASSTDDGYCCALAVEKYLKHYLDKIDVKTKKIPGLQVLDAELFRKEGVVNFVKCILKEIDGYDPSNFILNPTGGYKALVPYTVLLGMLKGIKCDYIFEQSSTLLELPPLPVEFKRSQFDTYQDLFEKIEHETEISQEEWDQRVPYKEREMLEPLVEFSNGHVTISAVGFLFLEEMRKPSVLVPFLSQKAIRDCFDNLAQLDNRDPFDFLLRAASSKDGFSQYEHINVGNGLRWLKPGNTTDRYLVSVEGWRMLVWRAIREDQEGPNYASKVKVNPDNDRRAYSPFMRMDFVN
>CP020114.1|AVZ31153.1|4054921_4055680_-|CRISPR-associated-endoribonuclease-Cas6
MLIHSTWTLSVSTSTVLPRSYGLELVKQLHYQLGLEMGSETIPSVSYSGIIGFASTSRDFITFHSEESYQLSLCGLNEVSAKAISHLNLGDSLEFLGAKFNIINREDEITSYEELYTNLVGNEPEPVRRFDLQFITPTAFAQGGVNLPLPLPTLMFRSWLERWNNFAPIYLGSDDLIAYLGEAIFIKNHKIQTRSYQLNKGFLNGFVGEVTLQVLNRADPLLANVANLLVQYARFSSTGMKTRLGMGQTKSI
>CP020114.1|AVZ31154.1|4055685_4056972_-|CRISPR-associated-protein-Csm5
MVVASESALKKPDFYESKRIQLTSPLLHIGSSVSKLNPFEYVQIAKKVYLPNQEALAKGLLKKGGSFLNDYIQVIEERQDITRLLKQAFGDEWWNAKDTDENPIFPKDAISQNFTQERITDLRPMIRNGMGYLFIPGSSIKGAIRTAIAYHLLKYPDRYQVPLEYRSSQIEDRLKNSLGELRNKHKQKFADDKLFMDNFFSNYTLNYQGKKLSGSSQNTDFLRCLKVSDSEPIIERKVPSKTGKSIPVNFSVVGEVIVSSRYPDYLAKYKASIYAEMARNVNTEFTLTLDTEMLSWFEHKQGMKLPFKNLNELLQICEDFAQEQWDYEHDYWNTIENNPRASGKNLDFNHIRDLYEPEKCPYTLRLGWGSGMTGTTVGLCFDDEMRAEIRDICGIKAPGFEAPKSRRTVMNSNGEIKFVPGWVKFRAL
>CP020114.1|AVZ31155.1|4056955_4058038_-|CRISPR-associated-protein-Csm4
MSVWKLVELNFERNLAHFGEVGIGMETTGDRIRSDSLFSAWVSNYARLFQKEGVEELLQLFPTDKQPQLIPPFRISSTFIYRQVGVNIIYYLPRPLKFPINYPDEDLAFFKAYKKLNYLPLEIWERWYQGTGFTTDDAEELEKHISDKTQGKLLATAETFNHSKACKIQQLPKIAIDRNTRATNIYHTGFVQFAWEKDKNPAGLYFLLELSPEGEKLADKLQAALHLLGEEGIGGERSSGAGRFKVSWLELPKNWQKVVNFQGGNHHTLMSLFWDDDISSDLLKNSSYEILERGGWIAESQLRRQMVRMFSEGSVFSTLPQGKLVDVKPKEFTKHSIYRSGISLSLPIKVQEKENHGCCV
>CP020114.1|AVZ31156.1|4058034_4059012_-|CRISPR-type-III-associated-RAMP-protein-Csm3
MPVSYEQTPLVGKVTLTCQLTAKTGLHIGGGGENLDIGGLDKPVIRDPLTKYPYLPGSSIKGKLRATLERLLKKPLNRTGGSGTWRYESDDLVDGFSEVETETFIRYEGAITCEISRLFGSTGGSNFWIPIETARNEGLIFDEIHTRTIENQNCVKINRGRNAPSRLIIRDCHLSPDSAEKLKKVDTGLYMTEWKFENGIDRVTAAANPRQLERVPAESKFDFELIYTVENQEQAVEDLQNIAIALAFLEDDALGGHGSRGYGKVKFENFRFNYRSLEQYRQIANATQESSALQTLDAIPNTQALLDNFRSLREYIQRLLPGNES
>CP020114.1|AVZ31157.1|4059014_4059566_-|CRISPR-associated-protein-Csm2
MNKPNKLQPPNTRNSGKIVSQSTNPSQSYSRVNRETNTNIVKEIKEEIGKSDYLKDYPIRILVQQSEDLGYYLKEQRLETNQIRKFLDAINQIKYILALDDDEKIKNAKTEDQEKLEFSKIETEIVLLKPKLAYAAARQNAVKPLNEVMKFAIDRVKTPQDFYRLVQFIESIIAYHKEADSRR
>CP020114.1|AVZ31158.1|4059568_4061860_-|CRISPR-associated-protein-Csm1
MVTSSEKVALQVIQQAISALAKWANFAHPPLDNLDKNEFPEVTRAKEILDWKPETKIGILSLVFDSVNLSDKPQKQTTKHYHPLKAIDNDDKEYPDIPYPLNSAPSSEQQQAFKTEINKALSKYLKDNWENLSLLMLILEKFGSCLSFGEADVALVDIARTTAGVAVALLNNPTEKISLIAGDLSGIQKFIYSISSEGALKSLRARSFYLELVTEEVVQQLLEQLNLPRTNIIYAGGGNLYILASGTADNQNIVQNIRQQFNEWLLAKFEGKVFLALDCLEFTVTELKDNFAKHWVKATKNLAVHKSRKFAENQISKFIAPRYSHEPCRVCHRDDVETLEPLNSEEPDSVLACGTCRSMFDLGSQLLDVKAIVRSTNESVRKGLPTLSFKINSREIHYHLFKNLEEINTDSDRVLLVNDWQLEHYEFKHFKNAAPLLLGNYGKKSEQKPENTEEPIGFMRAGEMSEKAKGANRVGYLRMDVDRLGQIFAKGLGKKQTLSRLAGLSRQMSYFFKVYLNTLAAYINLKNPDIIKPVKPDEQGLNILFIYAGGDDLFISGAWSEIVEFAFEIYRSFRTYTGNNEHITLSGGISIDDIKFPLYQAAKSSGKAEEAAKGNGRDSLGLFGEVFKWDEWEGRQDFKHLDDDVKKYLDSETLPSLFGVYPFVKRLEQQDIGVNYSRNFVRNLLITAEIQEQALKKFKENEKSQEALGTRYYLHLPKIAYTLARLPKKVLEDIDFRTSLKNPYNAPYFRAIATWIELLNRGN
>CP020114.1|AVZ31159.1|4062125_4062431_-|CRISPR-associated-protein-Csx3
MTTYNIEFKDGILRVRFGEPAQNDQIVKDAAARLEEMTTSGELTGGQLLKINGPISIPVAFVLAHKLSHIYGAIGLFDPKIGKYVISITHNPAYKLGDLID
>CP020114.1|AVZ31160.1|4066573_4066921_-|hypothetical-protein
MKYLTPEQVYKQFGYHPKTTAEWADLGKIECIRSPGGHRRYPESAFIKTVSTDKERVLYARVSTKTQLLDLDTQIEFLGKTYPGCRVVKDVASGMNWKRKNFLKLMTQVAPESNL

Crispr_ID: CP020114_14

• CRISPR_ID: CP020114_14
• CRISPR_location: 4154495-4154587
• CRISPR_type: Unclear
• Repeat_type: NA
• Spacer_info: 1 spacers
• Cas_protein_info: cas6

Consensus repeat of CP020114_14

Consensus_repeat	Method
AAGGAGAGGGGAGAATTTCAGAAA	CRISPRCasFinder

spacers of CP020114_14

>14.1|4154519|45|CP020114|CRISPRCasFinder
CCCCAACCCCTTTCCTTGCTTTCGCGTAGCGTCTCCCTTTGGGAG

CRISPR arrays and Neighbor proteins around CP020114_14

CRISPR arrays

The CRISPR arrays of CP020114_14

>merge|CP020114|14|4154495-4154587|CRISPRCasFinder
AAGGAGAGGGGAGAATTTCAGAAACCCCAACCCCTTTCCTTGCTTTCGCGTAGCGTCTCCCTTTGGGAGAAGGAGAGGGGAGAATTTCAGATA

>CP020114|14|14|4154495-4154587|CRISPRCasFinder
AAGGAGAGGGGAGAATTTCAGAAA	CCCCAACCCCTTTCCTTGCTTTCGCGTAGCGTCTCCCTTTGGGAG
AAGGAGAGGGGAGAATTTCAGATA

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
CP020114.1|AVZ31210.1|4159266_4160757_-|hypothetical-protein	unknown	unknown	unknown
CP020114.1|AVZ31209.1|4158732_4159068_-|hypothetical-protein	unknown	unknown	gnl|CDD|319862
CP020114.1|AVZ31204.1|4154636_4154993_-|hypothetical-protein	unknown	unknown	unknown
CP020114.1|AVZ31196.1|4135210_4136599_-|putative-cytochrome-P450	unknown	unknown	gnl|CDD|225035
CP020114.1|AVZ31206.1|4155480_4156191_-|GTP-cyclohydrolase-1	unknown	unknown	gnl|CDD|236472
CP020114.1|AVZ31205.1|4155065_4155248_-|cysteine--tRNA-ligase	unknown	unknown	gnl|CDD|234705
CP020114.1|AVZ31207.1|4156248_4157283_-|putative-metal-chaperone-YciC	unknown	unknown	gnl|CDD|223597
CP020114.1|AVZ31202.1|4151798_4152821_-|photosystem-I-assembly-protein-Ycf3	unknown	unknown	gnl|CDD|276809
CP020114.1|AVZ31200.1|4145503_4146514_-|biotin-synthase	unknown	unknown	gnl|CDD|215219
CP020114.1|AVZ31211.1|4161258_4161651_-|toxin-Doc	unknown	unknown	gnl|CDD|226180
CP020114.1|AVZ31194.1|4131820_4132012_-|hypothetical-protein	unknown	unknown	unknown
CP020114.1|AVZ31197.1|4136744_4138202_-|alpha-amylase	unknown	unknown	gnl|CDD|236518
CP020114.1|AVZ31212.1|4161647_4161872_-|hypothetical-protein	unknown	unknown	gnl|CDD|274230
CP020114.1|AVZ31208.1|4157433_4158579_-|CRISPR-associated-endoribonuclease-Cas6	cas6	COG5551_cas6_CAS-III	gnl|CDD|227838
CP020114.1|AVZ31201.1|4147343_4150235_-|pyruvate,-water-dikinase	unknown	unknown	gnl|CDD|235751
CP020114.1|AVZ31198.1|4138402_4139215_-|HAD-superfamily-phosphatase	unknown	unknown	gnl|CDD|273685
CP020114.1|AVZ31213.1|4162007_4162646_-|tyrosine-recombinase-XerD	unknown	unknown	gnl|CDD|271175
CP020114.1|AVZ31199.1|4143953_4145375_-|serine/threonine-protein-kinase-B	unknown	unknown	gnl|CDD|270916
CP020114.1|AVZ31195.1|4132120_4132894_-|imidazole-glycerol-phosphate-synthase-subunit-HisF	unknown	unknown	gnl|CDD|234996
CP020114.1|AVZ31203.1|4153119_4153704_-|hypothetical-protein	unknown	unknown	unknown

HMMScan for Signature genes

Protein	Cas_name	Cas_description	E-value	Identity	Coverage
CP020114.1|AVZ31208.1|4157433_4158579_-|CRISPR-associated-endoribonuclease-Cas6	cas6	COG5551_cas6_CAS-III	4.5e-75	247.3	0.1

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
CP020114.1|AVZ31209.1|4158732_4159068_-|hypothetical-protein	gnl|CDD|319862	cd16383, GUN4, porphyrin-binding protein domain GUN4. GUN4 is a porphyrin-binding protein involved in chlorophyll biosynthesis regulation and intracellular signaling, found in aerobic photosynthetic organisms. It has been implicated in retrograde signalling between the chloroplast and nucleus. GUN4 can bind protoporphyrin IX (PIX) and magnesium protoporphyrin IX (MgPIX), substrate and product of the Mg-chelatase, as well as heme and cobalt protoporphyrin IX (CoPIX). It may play a role in protecting plants from reactive oxygen species (ROS)-mediated damage.	1.22091e-22
CP020114.1|AVZ31207.1|4156248_4157283_-|putative-metal-chaperone-YciC	gnl|CDD|223597	COG0523, COG0523, Putative GTPases (G3E family) [General function prediction only].	2.09683e-114
CP020114.1|AVZ31196.1|4135210_4136599_-|putative-cytochrome-P450	gnl|CDD|225035	COG2124, CypX, Cytochrome P450 [Secondary metabolites biosynthesis, transport, and catabolism].	5.31837e-104
CP020114.1|AVZ31202.1|4151798_4152821_-|photosystem-I-assembly-protein-Ycf3	gnl|CDD|276809	sd00006, TPR, Tetratricopeptide repeat. The Tetratricopeptide repeat (TPR) typically contains 34 amino acids and is found in a variety of organisms including bacteria, cyanobacteria, yeast, fungi, plants, and humans. It is present in a variety of proteins including those involved in chaperone, cell-cycle, transcription, and protein transport complexes. The number of TPR motifs varies among proteins. Those containing 5-6 tandem repeats generate a right-handed helical structure with an amphipathic channel that is thought to accommodate an alpha-helix of a target protein. It has been proposed that TPR proteins preferentially interact with WD-40 repeat proteins, but in many instances several TPR-proteins seem to aggregate to multi-protein complexes.	2.01148e-07
CP020114.1|AVZ31200.1|4145503_4146514_-|biotin-synthase	gnl|CDD|215219	PLN02389, PLN02389, biotin synthase.	2.12118e-167
CP020114.1|AVZ31201.1|4147343_4150235_-|pyruvate,-water-dikinase	gnl|CDD|235751	PRK06241, PRK06241, phosphoenolpyruvate synthase; Validated.	5.20416e-91
CP020114.1|AVZ31211.1|4161258_4161651_-|toxin-Doc	gnl|CDD|226180	COG3654, Doc, Prophage maintenance system killer protein [General function prediction only].	1.46059e-31
CP020114.1|AVZ31197.1|4136744_4138202_-|alpha-amylase	gnl|CDD|236518	PRK09441, PRK09441, cytoplasmic alpha-amylase; Reviewed.	0
CP020114.1|AVZ31208.1|4157433_4158579_-|CRISPR-associated-endoribonuclease-Cas6	gnl|CDD|227838	COG5551, COG5551, CRISPR system related protein, RAMP superfamily [Defense mechanisms].	4.84885e-58
CP020114.1|AVZ31205.1|4155065_4155248_-|cysteine--tRNA-ligase	gnl|CDD|234705	PRK00260, cysS, cysteinyl-tRNA synthetase; Validated.	8.13186e-09
CP020114.1|AVZ31198.1|4138402_4139215_-|HAD-superfamily-phosphatase	gnl|CDD|273685	TIGR01548, Imidazoleglycerol-phosphate_dehydratase, haloacid dehalogenase superfamily, subfamily IA hydrolase, TIGR01548. This model represents a small and phylogenetically curious clade of sequences. Sequences are found from Halobacterium (an archaeon), Nostoc and Synechococcus (cyanobacteria) and Phytophthora (a stramenophile eukaryote). These appear to be members of the haloacid dehalogenase (HAD) superfamily of aspartate-nucleophile hydrolases by general homology and the conservation of all of the recognized catalytic motifs. The variable domain is found in between motifs 1 and 2, indicating membership in subfamily I and phylogeny and prediction of the alpha helical nature of the variable domain (by PSI-PRED) indicate membership in subfamily IA. All but the Halobacterium sequence currently found are annotated as "Imidazoleglycerol-phosphate dehydratase", however, the source of the annotation could not be traced and significant homology could not be found between any of these sequences and known IGPD's.	1.41051e-70
CP020114.1|AVZ31206.1|4155480_4156191_-|GTP-cyclohydrolase-1	gnl|CDD|236472	PRK09347, folE, GTP cyclohydrolase I; Provisional.	7.17085e-119
CP020114.1|AVZ31213.1|4162007_4162646_-|tyrosine-recombinase-XerD	gnl|CDD|271175	cd00397, DNA_BRE_C, DNA breaking-rejoining enzymes, C-terminal catalytic domain. The DNA breaking-rejoining enzyme superfamily includes type IB topoisomerases and tyrosine based site-specific recombinases (integrases) that share the same fold in their catalytic domain containing conserved active site residues. The best-studied members of this diverse superfamily include Human topoisomerase I, the bacteriophage lambda integrase, the bacteriophage P1 Cre recombinase, the yeast Flp recombinase, and the bacterial XerD/C recombinases. Their overall reaction mechanism is essentially identical and involves cleavage of a single strand of a DNA duplex by nucleophilic attack of a conserved tyrosine to give a 3' phosphotyrosyl protein-DNA adduct. In the second rejoining step, a terminal 5' hydroxyl attacks the covalent adduct to release the enzyme and generate duplex DNA. The enzymes differ in that topoisomerases cleave and then rejoin the same 5' and 3' termini, whereas a site-specific recombinase transfers a 5' hydroxyl generated by recombinase cleavage to a new 3' phosphate partner located in a different duplex region. Many DNA breaking-rejoining enzymes also have N-terminal domains, which show little sequence or structure similarity.	9.94452e-25
CP020114.1|AVZ31199.1|4143953_4145375_-|serine/threonine-protein-kinase-B	gnl|CDD|270916	cd14014, STKc_PknB_like, Catalytic domain of bacterial Serine/Threonine kinases, PknB and similar proteins. STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. This subfamily includes many bacterial eukaryotic-type STKs including Staphylococcus aureus PknB (also called PrkC or Stk1), Bacillus subtilis PrkC, and Mycobacterium tuberculosis Pkn proteins (PknB, PknD, PknE, PknF, PknL, and PknH), among others. S. aureus PknB is the only eukaryotic-type STK present in this species, although many microorganisms encode for several such proteins. It is important for the survival and pathogenesis of S. aureus as it is involved in the regulation of purine and pyrimidine biosynthesis, cell wall metabolism, autolysis, virulence, and antibiotic resistance. M. tuberculosis PknB is essential for growth and it acts on diverse substrates including proteins involved in peptidoglycan synthesis, cell division, transcription, stress responses, and metabolic regulation. B. subtilis PrkC is located at the inner membrane of endospores and functions to trigger spore germination. Bacterial STKs in this subfamily show varied domain architectures. The well-characterized members such as S. aureus and M. tuberculosis PknB, and B. subtilis PrkC, contain an N-terminal cytosolic kinase domain, a transmembrane (TM) segment, and mutliple C-terminal extracellular PASTA domains. The PknB subfamily is part of a larger superfamily that includes the catalytic domains of other protein STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase.	6.76541e-55
CP020114.1|AVZ31195.1|4132120_4132894_-|imidazole-glycerol-phosphate-synthase-subunit-HisF	gnl|CDD|234996	PRK02083, PRK02083, imidazole glycerol phosphate synthase subunit HisF; Provisional.	9.65379e-179
CP020114.1|AVZ31212.1|4161647_4161872_-|hypothetical-protein	gnl|CDD|274230	TIGR02609, hypothetical_protein_XAC1195, putative addiction module antidote. Members of this protein family are putative addiction module antidote proteins that appear recurringly in two-gene operons with members of the Doc (death-on-curing) family TIGR01550. Members of this family contain a SpoVT/AbrB-like domain (pfam04014). Note that the gene pairs with a member of this family tend to be found on bacterial chromosomes, not on plasmids. [Mobile and extrachromosomal element functions, Other].	2.67719e-19

Protein sequences

>CP020114.1|AVZ31203.1|4153119_4153704_-|hypothetical-protein
MADFTSTLSQAVAAYRGQGGARPTAQTVVNALLQAETAAKQERLTYPFASLLGKWQLCFATGTKKARKRGGIILGKGLYVPKFVAVQISFSSQPDSTKGEIGNQVQLGPLLLHLTGPAKYLGKKNLLAFDFNYLVFSLFGRMVYNKQIRSGQAQSEDFYEQSIAKLPFFAFFLVTPDFIAARGRGGGLALWIRH
>CP020114.1|AVZ31202.1|4151798_4152821_-|photosystem-I-assembly-protein-Ycf3
MTQAFNQVQEWEQRRNEASRYYKQGQFQEYLNFASENLQLARSIPDRAREGHTLNDIGLAHLSCCQPLQALECFQQARLVATELGNRRAEAIALSNLGSTYSRLGRLSRSLEYFAQALGIFRDLQDIPGEVSTLNDVALIYTKLGQPKRSLLIQYQVLSMRRTLGDFSGEATTLNGIGYAYSTLGQYERALEFLQAALPIQRAVKNVMGEASTLNNIASVYGDLGEPKKALLLYYQVLLTRRAIKDRAGEGTTLQNIGFTYGILANNRQAMKYYEQAIAIYQEIGDCLGEISTLLNMGNFYAKTKRKKLALSYYHNAHDLAKAIESQPQLQKIKQCIDAL
>CP020114.1|AVZ31201.1|4147343_4150235_-|pyruvate,-water-dikinase
MIELWGALIILIACPVLGALPIIGWITYALKGRQLSQIGTKNISVSAAFYHGGTWVGILAVLSEAIKGIAAVLLSRVFFPEGSVWELIALIALVLGRYGVGRGAGTTNVVWGFIVHDPLVAIFTSLLAIISFTLLQSKLLVKFGVLFVFPLFVILLNVDNLPKIMAAIVLAGVLGWIYTKIPDDLNLPPEEADTDSQAAFTYLRGNRRILTLDDELDATVFGEKAATLSQIKRWGYPVPKGWVMSPEDDPQGLIAMLEPSDLSPLVVRSSAIGEDSEQASAAGQYTTVLNVTSKQELQGAIAQVQASYNHPSAVQYRRDRGLPDTAMAVLVQQQIQSVYSGVAFSRDPITQQGDAIVIEALPGSANQVVSGKVTPEQYRAFVVETENISSVQVEGSGQVPQALIKQVVYLVRRLEKQYHGLPQDIEWTYDSQNLWVLQSRPITTLLPIWTRKIAAEVIPGVIQPLTWSINRPLTCGVWGDFFTLVLGDRALGLDFTETATLHYSRAYFNASLLGQIFLRMGLPPESLEFLTRGAKMSKPQLKTTLANLPGLMRLLKQELSLEKDFKRDYSRKFIPGMSQLAHESIEELRAPQLLERIDFILELLRSGTYYSILAPLSAALRQAVFRVKDGEIDNSIAPEVAALRSLTALAADAKKILPECEPDLVFEQLAQTPEGEQILDEFSELLEDYGYLSDVGTNIAVPTWKENPQPIKQLFVQLLQNPPETDGGDSINRVLSAKRKRGVVQRRVDIKGRVTELYSRLLAELRWRFVALEKIWLKSGLIQQTGDIFFLELDEVRRLVAGDDLELRKDLSEIVELRRSQFHQDSQFNQIPLLVYGHTPPHPLPPSSLYSDQVLQGIPASHGQAEGRIKIVRNLQDLPEINRETILVVPYTDSGWAPLLVRAGGLIAEAGGRLSHGAIVAREYGIPAIMDVHNATWILQDGQRVRMDGSRGIIELSHDLRPD
>CP020114.1|AVZ31200.1|4145503_4146514_-|biotin-synthase
MSVGIRYNWQKAEIQAIYNTPFLELIYQAASVHRQYHDSTKIQVCKLISIKTGACPEDCGYCAQSSRYQTEVKPQALLEKETVLNIAQKAKATGVSRVCMGAAWREVRDNSQFETVLEMVKEVTDMGLEVCCTLGMLTTNQAKRLEAAGLYAYNHNLDTSEEYYNTIITTRTYGDRLNTIDNVRQTNVTVCSGGILGLGESVEDRVGMLETLSNLQPHPESVPINILSQVPGTPLENQPDVPIWDVVRMIATARIIMPNSDVRLSAGRARLSQVEQALCFMAGANSIFSSDDNQMLTVTTPCPDYDADKEMLNLLGLEMRPPHQRPEKVTTPAVVA
>CP020114.1|AVZ31199.1|4143953_4145375_-|serine/threonine-protein-kinase-B
MSYCINPQCPQPQNTRQPLFCQSCGSELLLEGCYRVIRTLGGGGFANTYEVDDSGTAKVLKVLFNTHPKAVELFQKEAEVLSILQHPGIPKVDSDGGYFTYLSRNQEPLHCLVMEKIEGMNLEEYLTQRHYQPISERAAVRWLKQLAEILHKVHQQQYFHRDIKPPNIMIRPNGQLVLIDFGTAREVTQTFINKVAGQQVTGIISAGYTPREQMNGKAVPQSDFFALGRTFVYLLTGKSPDSFSEDPSNGELIWRDHAAGISDKLVEFIDYLMAPFPGNRPQDTQEILHKLAKFDTPSPAFGQNTGNNQSANYYNQISQTIASKSPAVNQGVNYSPYYAGFKRRTLAYFIDILITVFILFIASFIMVEYSYSIDVIILIGWIIPTWLYFAVFESSEKQATLGKMVGKIVVTDLEGKTISFWRATGRFLVKAFFTLLTLIGFGLIDVIFILFNKNKRSLHDILAGTLVTKKPSP
>CP020114.1|AVZ31198.1|4138402_4139215_-|HAD-superfamily-phosphatase
MTEKYSLNPIVIFDIDGVVRDVGNSYRRALADTVEHFTNTAYRPTPVDIDQLKSEGLWNNDWEASQEFIYRHFETLGQTRQQLQLDYKTIVAFFQSRYRGSDPENWNGYICDEPLLLQPSYLEQLTEAGIYWGFFSGATRGSATYVLHKRLGLQSPVLIAMEDAPGKPDPTGLFATIGLLETEINKMSAIIYVGDTVADMYTVRKAREMHPHRIWIGVGILPPHVQETAAHSDAYSQTLLAAGAAIVLSNVEQLNPVKVQELLQGMGIRG
>CP020114.1|AVZ31197.1|4136744_4138202_-|alpha-amylase
MSNGVMMQYFHWYNPNDGNLWNQLDKTAHELVNAGFTALWLPPPYKGSGGSYDVGYGVYDLFDLGEFDQKGSVRTKYGTREQYLEAIKTAQSSGLHIYGDVVLNHKDGGDETERIWAQEMDWNDRNRPVSDWYEIDAYTKFHFSGRGDKYSSMKWYWWCFDSLSYNAGTQKADKLYRIKNREFETEVSPEHGNYDYLLANDLDMGEEPVRGELMYWGRWFVDTTNVNGFRIDAVKHIRSSFFRDWLNHLRVHFSQRQLFSCGEYWSQNIDELHGYLAATTGVMSLFDVPLHFKFHQASRQGSGFDMRTIFNRTLAKEQPALAVTFVENHDTQPCQSLESPVEPWFKPLAYALILLRREGYPCVFYADYYGAQYHDKGRDATLYSHRFLIDKFLGARRDYGFGDQHDYFDHPHTIGWTRLGNSEHPGAMAVVMTNSEAGNKWMNMFRPNEAFYDATGHIQEIVYTNNDGWGNFFCPGGSVSVWLQK
>CP020114.1|AVZ31196.1|4135210_4136599_-|putative-cytochrome-P450
MPALQLPDGPKNHPWLQTYRWLTSPLEYMEDCAKNYGDIFTIRVGPLSTPQVFVSNPQAIQQIFSTDPKYLDSGAAAGFKSPLLGNQSLLSLDGKPHQRQRKLLTPPFHGERMLAYGELIRDISQQVTNKWQVGETVSVLSSMQAISFQVILKAVFGLAEGPRYEKIKEALIAILNPKKPLLRSMLLMFPSLRRDLGAWSPWGEFLRLRQQIDELVYAEIQERKAQLDSSRTDILSLMMATRDEAGEPMTDLELRDELMTLLVAGHETTATALSWALYWIHHQPQVREKLLQELDTLGEKPDPNAIFRLPYLNAVCSETLRLYPVAMLLLSRLVKSPLQIGEYQFEPGTLLIPCVYLTHHREDLYPDSQTFKPERFLERQFSNSEFIPFGGGNRRCIGMAFALFEMKLVLATVLSNWEMELANTQPVLPVRKGLLFGPKGGVQMVVKGRREQNQLVPETTSV
>CP020114.1|AVZ31195.1|4132120_4132894_-|imidazole-glycerol-phosphate-synthase-subunit-HisF
MLSKRILPCLDVKAGRVVKGVNFVDLKDAGDPVELAKVYNEAGADELVFLDITATHEDRDTIIDVVYRTAEQVFIPLTVGGGIQTLENVKALLRAGADKVSINSAAVRDPDLINRASDRFGNQCIVVAIDARRRIDPHNPGWDVYVRGGRENTGLDALRWAQEVERRGAGELLVTSMDADGTQAGYDLGLTRAIAESVQVPVVASGGAGNCEHIYTALTEGKAEAALLASLLHYGQLSVAEIKNYLHAHSVPVRLCA
>CP020114.1|AVZ31194.1|4131820_4132012_-|hypothetical-protein
MLIVILLFDVALVAWSLHLMEKAVEHKEFSLMLAGTLVALAAAAMLVVYFLMGHCMTYLLQVS
>CP020114.1|AVZ31204.1|4154636_4154993_-|hypothetical-protein
MNKKTILSLLSTPMLVSSILSMGAMANQAQAANPETENTDKLTCVQNYHILRPVCARASVLAQIPEYEPANELAGENQDSLLAFNIEESDMAVDLFNCDCPQCINSLRQMRGIAPLVY
>CP020114.1|AVZ31205.1|4155065_4155248_-|cysteine--tRNA-ligase
MERLYRGFDIITNPVHTSNQQRPKTGKAENFAASDRIRDQLQELGTTLIDQSDGTTRWHS
>CP020114.1|AVZ31206.1|4155480_4156191_-|GTP-cyclohydrolase-1
MPTEISQLTNSAKHSYKTQKMTLSIRPDLTSADQVISSLTAQKQPKVTEAEMMQAVRTLLIGLGEDPDREGLKDTPKRVMKALQFLTKGYTDSLDELLNGAVFTEDANEMVLVRDIDIFSSCEHHILPIIGRAHVAYIPNGKVIGLSKIARICEMYGRRLQVQERLTTQIADALQGLLKPQGVAVVVEATHMCMVMRGVQKPGSWTVSSAMRGVFAEDSRTREEFMNLIRHNANFH
>CP020114.1|AVZ31207.1|4156248_4157283_-|putative-metal-chaperone-YciC
MTTLTPSATNVTPEIPKRGMPVTLITGFLGSGKTTLLNQILKNKENLKVAVLVNEFGDINIDSQLLVSVDQDMMELSNGCICCTINDGLVDAVYRILEREERIDYLVIETTGVADPLPIILTFLGTELRDLTNLDSIITLVDVDAFDAEHFQSESALKQITYGDIILLNKVDLATPEKVKEVESYIHEVKAGAKILPTQYGEVPLALILDTQLTPQSQYISIAEQDAHQEHHHEHDHHDHEHHHHHSDHLDNDGFISISFQSDRPFDVHKFEAFLTEQMPAGVFRAKGILWFNDSDLRHIFQLSGQRYNLHADDWYSQPKNQLVFIGRNLKTNEIHSHLHKCLV
>CP020114.1|AVZ31208.1|4157433_4158579_-|CRISPR-associated-endoribonuclease-Cas6
MVRTAKPTNRQQKPKSSPTATLPTWADNTELVGLEFDLEALTTSSLYSQYTIALHAWFLDQVRQLDPDLSAYLHDGESEKPFNISALESQLLPTGKQLQLEANQILHWQVNALSAKVAEFLQLWLTQLPQTLNLRGATLQIKQVRIAHPPTTYAQLLQPPAKYSQVNLSFISPTSFRRKGHHFPLPVPVNLFHSYLRRWNDFSQIPISQADFLDWIDENVIIHQHRLESVKVAAGKRGSVTGFTGAISCGLSKAALANTEFTQLFYALVKLAPYCGTGHKTTFGLGQTSLSWVEPEASSPTQLLTNLLGERIEELTAIFTAQRKRSGGDRTDKIAATWATILARREMGESLKLIAEDLEMPVDTVKTYTKLARRSLKDADL
>CP020114.1|AVZ31209.1|4158732_4159068_-|hypothetical-protein
MISDRYFLTQLHKIALLPGRFGFSAQKKKYLEVGGTRTYRNGTWEKYCKLVGWYTKGYFGNYIGEGDLIYDISAPEGHLPWVWLYRNLWYCNEQTRVTYPALILREDLIIR
>CP020114.1|AVZ31210.1|4159266_4160757_-|hypothetical-protein
MTKNICASLDLNTSIKTFQSNATKLLDFANIPEWDGKKLKEREEEIRVQALILAGQCMAILLYNLSISPKILDYSVAQTQGWRSTKTQKHGYKKRQIVTIGNVEVTLNLPYVLERNPKSKKSDSSSLNKQKIKTSNQGFCPFLKWLGMSEGITPLVWSTIAKYGTIASSFDAAHSTLIDWGINVSLKRIERLTYLFGKIGINLRQSKILHLEMDNLSDSNVLKDQRVVIAVDGGRTKIRFNKKGRPSSKTNRHGFVGEWMEPKLLTIYVVNEQGKKIRTSAIPITNDGTYSGYKEFLKILEMYLVNLGISQAKQVLLIADGAEWIWIHIPPLLKKLKCPSETYQLLDLYHAVSHLKDFADAAFSTDNESQLWFKKARKALKKGQTLDLMRNMGEFISGATGERCKILVRERNYILKAYRRRLLKYNEVASQKLPLGSGAVESLIRQVVNLRMKGNSKFWLQNNAEIMLHLRCQWIAKTWDNFCDSIFNSLIKSPTG
>CP020114.1|AVZ31211.1|4161258_4161651_-|toxin-Doc
MNGIFWLDETIVTVIHEDQLAQHGGLAGVRDNNLFLASLDRPKNLLAYGEPTPTIFDLAAAYGYGFAKNHAFIDGNKRVAFAVMATFLELNGYSLDVPEPQVVLIMERLANGQESQETISEWLQENSIKY
>CP020114.1|AVZ31212.1|4161647_4161872_-|hypothetical-protein
MYTLKIRRVGNSLGVTLPKEALQKLRVQEGDSVFVTETSSGVHVTACNPDFEKAMSAYRKVSTKYRNALHELAK
>CP020114.1|AVZ31213.1|4162007_4162646_-|tyrosine-recombinase-XerD
MWGRSHGLQSVMKINRHGRAKVITQQEIQLIFTHGLNNDRDRMLFGVCLFSACRIRECVTLQTQDIYTPKGEVRSQFVIRKSNTKGKLATRSIPVIEDLRRLLADYSRLAGNVYLFPGRSNGHISHDSAARILRKACEQVGIIGVSTHSFRRTALTQMSNAGIPLRVIQELSGHRNLEQLQRYLEVSDDQVLGAAAALSMLSPAVEVSKYKI

Crispr_ID: CP020114_15

• CRISPR_ID: CP020114_15
• CRISPR_location: 4979869-4980088
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 3 spacers

Consensus repeat of CP020114_15

Consensus_repeat	Method
GAAATTTCCCTTAAGAATGAGAATAGTGATTGTC	CRT

spacers of CP020114_15

>15.1|4979903|29|CP020114|CRT
GGGAGGGGGATGCTCTCACCATTAACTCG
>15.2|4979966|26|CP020114|CRT
CCGAAGGGGATCGCGCTAAAGATATA
>15.3|4980026|29|CP020114|CRT
AGGAGGGGGATGATCTCACCATTAACTCG

CRISPR arrays and Neighbor proteins around CP020114_15

CRISPR arrays

The CRISPR arrays of CP020114_15

>merge|CP020114|15|4979869-4980088|CRT
GGACTTTCCCTTAAGAATGAGAATAGTGATTGTCGGGAGGGGGATGCTCTCACCATTAACTCGGAAATTTCCCTCAAGAATGAGAACAGTGATTGTCCCGAAGGGGATCGCGCTAAAGATATAAAAATTTCCCTTAAGAATGAGAATAGTGATTGTCAGGAGGGGGATGATCTCACCATTAACTCGGAAATTTCCCTTAAGAATGAGAATAGTGATTTTC

>CP020114|15|3|4979869-4980088|CRT
GGACTTTCCCTTAAGAATGAGAATAGTGATTGTC	GGGAGGGGGATGCTCTCACCATTAACTCG
GAAATTTCCCTCAAGAATGAGAACAGTGATTGTC	CCGAAGGGGATCGCGCTAAAGATATA
AAAATTTCCCTTAAGAATGAGAATAGTGATTGTC	AGGAGGGGGATGATCTCACCATTAACTCG
GAAATTTCCCTTAAGAATGAGAATAGTGATTTTC

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
CP020114.1|AVZ31568.1|4949309_4950242_-|hypothetical-protein	unknown	unknown	gnl|CDD|376573
CP020114.1|AVZ31576.1|4983959_4985816_-|two-component-system,-sensor-histidine-kinase-and-response-regulator	unknown	unknown	gnl|CDD|223715
CP020114.1|AVZ31581.1|5003937_5004459_-|hypothetical-protein	unknown	unknown	gnl|CDD|371466
CP020114.1|AVZ31580.1|4996292_4997195_-|lipoyl-synthase	unknown	unknown	gnl|CDD|235493
CP020114.1|AVZ31565.1|4942197_4942734_-|hypothetical-protein	unknown	unknown	unknown
CP020114.1|AVZ31571.1|4970102_4971116_-|hypothetical-protein	unknown	unknown	unknown
CP020114.1|AVZ31566.1|4942746_4943178_-|hypothetical-protein	unknown	unknown	gnl|CDD|379326
CP020114.1|AVZ31582.1|5004800_5005010_-|hypothetical-protein	unknown	unknown	gnl|CDD|376462
CP020114.1|AVZ31567.1|4948522_4949002_-|RNA-polymerase-nonessential-primary-like-sigma-factor	unknown	unknown	gnl|CDD|180321
CP020114.1|AVZ31577.1|4991480_4991936_-|ferric-uptake-regulation-protein	unknown	unknown	gnl|CDD|366663
CP020114.1|AVZ31569.1|4952001_4952685_-|3-oxoacyl-[acyl-carrier-protein]-reductase-FabG	unknown	unknown	gnl|CDD|212491
CP020114.1|AVZ31574.1|4981301_4981772_-|putative-adenylyltransferase/sulfurtransferase-MoeZ	unknown	unknown	gnl|CDD|238089
CP020114.1|AVZ31578.1|4993634_4994885_-|RNA-polymerase-nonessential-primary-like-sigma-factor	unknown	unknown	gnl|CDD|236067
CP020114.1|AVZ31579.1|4995343_4996276_-|glycerol-3-phosphate-dehydrogenase	unknown	unknown	gnl|CDD|237771
CP020114.1|AVZ31563.1|4936385_4936727_-|putative-transcriptional-regulator	unknown	unknown	gnl|CDD|226181
CP020114.1|AVZ31564.1|4941819_4942194_-|hypothetical-protein	unknown	unknown	unknown
CP020114.1|AVZ31573.1|4980781_4981093_-|hypothetical-protein	unknown	unknown	unknown
CP020114.1|AVZ31572.1|4971626_4973465_-|serine/threonine-protein-kinase-B	unknown	unknown	gnl|CDD|270916
CP020114.1|AVZ31570.1|4959390_4962063_-|hypothetical-protein	unknown	unknown	gnl|CDD|379818
CP020114.1|AVZ31575.1|4982369_4983881_-|15-cis-phytoene-desaturase	unknown	unknown	gnl|CDD|225885

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
CP020114.1|AVZ31568.1|4949309_4950242_-|hypothetical-protein	gnl|CDD|376573	pfam01609, DDE_Tnp_1, Transposase DDE domain. Transposase proteins are necessary for efficient DNA transposition. This domain is a member of the DDE superfamily, which contain three carboxylate residues that are believed to be responsible for coordinating metal ions needed for catalysis. The catalytic activity of this enzyme involves DNA cleavage at a specific site followed by a strand transfer reaction. This family contains transposases for IS4, IS421, IS5377, IS427, IS402, IS1355, IS5, which was original isolated in bacteriophage lambda.	6.90209e-06
CP020114.1|AVZ31576.1|4983959_4985816_-|two-component-system,-sensor-histidine-kinase-and-response-regulator	gnl|CDD|223715	COG0642, BaeS, Signal transduction histidine kinase [Signal transduction mechanisms].	1.73571e-56
CP020114.1|AVZ31581.1|5003937_5004459_-|hypothetical-protein	gnl|CDD|371466	pfam11320, DUF3122, Protein of unknown function (DUF3122). This family of proteins with unknown function appear to be restricted to Cyanobacteria.	2.62707e-64
CP020114.1|AVZ31580.1|4996292_4997195_-|lipoyl-synthase	gnl|CDD|235493	PRK05481, PRK05481, lipoyl synthase; Provisional.	0
CP020114.1|AVZ31566.1|4942746_4943178_-|hypothetical-protein	gnl|CDD|379326	pfam13673, Acetyltransf_10, Acetyltransferase (GNAT) domain. This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.	0.00997071
CP020114.1|AVZ31582.1|5004800_5005010_-|hypothetical-protein	gnl|CDD|376462	pfam01124, MAPEG, MAPEG family. This family is has been called MAPEG (Membrane Associated Proteins in Eicosanoid and Glutathione metabolism). It includes proteins such as Prostaglandin E synthase. This enzyme catalyzes the synthesis of PGE2 from PGH2 (produced by cyclooxygenase from arachidonic acid). Because of structural similarities in the active sites of FLAP, LTC4 synthase and PGE synthase, substrates for each enzyme can compete with one another and modulate synthetic activity.	2.50147e-09
CP020114.1|AVZ31567.1|4948522_4949002_-|RNA-polymerase-nonessential-primary-like-sigma-factor	gnl|CDD|180321	PRK05949, PRK05949, RNA polymerase sigma factor; Validated.	1.49269e-69
CP020114.1|AVZ31577.1|4991480_4991936_-|ferric-uptake-regulation-protein	gnl|CDD|366663	pfam01475, FUR, Ferric uptake regulator family. This family includes metal ion uptake regulator proteins, that bind to the operator DNA and controls transcription of metal ion-responsive genes. This family is also known as the FUR family.	3.61712e-49
CP020114.1|AVZ31569.1|4952001_4952685_-|3-oxoacyl-[acyl-carrier-protein]-reductase-FabG	gnl|CDD|212491	cd05233, SDR_c, classical (c) SDRs. SDRs are a functionally diverse family of oxidoreductases that have a single domain with a structurally conserved Rossmann fold (alpha/beta folding pattern with a central beta-sheet), an NAD(P)(H)-binding region, and a structurally diverse C-terminal region. Classical SDRs are typically about 250 residues long, while extended SDRs are approximately 350 residues. Sequence identity between different SDR enzymes are typically in the 15-30% range, but the enzymes share the Rossmann fold NAD-binding motif and characteristic NAD-binding and catalytic sequence patterns. These enzymes catalyze a wide range of activities including the metabolism of steroids, cofactors, carbohydrates, lipids, aromatic compounds, and amino acids, and act in redox sensing. Classical SDRs have an TGXXX[AG]XG cofactor binding motif and a YXXXK active site motif, with the Tyr residue of the active site motif serving as a critical catalytic residue (Tyr-151, human prostaglandin dehydrogenase (PGDH) numbering). In addition to the Tyr and Lys, there is often an upstream Ser (Ser-138, PGDH numbering) and/or an Asn (Asn-107, PGDH numbering) contributing to the active site; while substrate binding is in the C-terminal region, which determines specificity. The standard reaction mechanism is a 4-pro-S hydride transfer and proton relay involving the conserved Tyr and Lys, a water molecule stabilized by Asn, and nicotinamide. Extended SDRs have additional elements in the C-terminal region, and typically have a TGXXGXXG cofactor binding motif. Complex (multidomain) SDRs such as ketoreductase domains of fatty acid synthase have a GGXGXXG NAD(P)-binding motif and an altered active site motif (YXXXN). Fungal type ketoacyl reductases have a TGXXXGX(1-2)G NAD(P)-binding motif. Some atypical SDRs have lost catalytic activity and/or have an unusual NAD(P)-binding motif and missing or unusual active site residues. Reactions catalyzed within the SDR family include isomerization, decarboxylation, epimerization, C=N bond reduction, dehydratase activity, dehalogenation, Enoyl-CoA reduction, and carbonyl-alcohol oxidoreduction.	7.10203e-51
CP020114.1|AVZ31574.1|4981301_4981772_-|putative-adenylyltransferase/sulfurtransferase-MoeZ	gnl|CDD|238089	cd00158, RHOD, Rhodanese Homology Domain (RHOD); an alpha beta fold domain found duplicated in the rhodanese protein. The cysteine containing enzymatically active version of the domain is also found in the Cdc25 class of protein phosphatases and a variety of proteins such as sulfide dehydrogenases and certain stress proteins such as senesence specific protein 1 in plants, PspE and GlpE in bacteria and cyanide and arsenate resistance proteins. Inactive versions (no active site cysteine) are also seen in dual specificity phosphatases, ubiquitin hydrolases from yeast and in sulfuryltransferases, where they are believed to play a regulatory role in multidomain proteins.	5.01086e-23
CP020114.1|AVZ31579.1|4995343_4996276_-|glycerol-3-phosphate-dehydrogenase	gnl|CDD|237771	PRK14619, PRK14619, NAD(P)H-dependent glycerol-3-phosphate dehydrogenase; Provisional.	0
CP020114.1|AVZ31563.1|4936385_4936727_-|putative-transcriptional-regulator	gnl|CDD|226181	COG3655, COG3655, Predicted transcriptional regulator [Transcription].	5.4053e-18
CP020114.1|AVZ31578.1|4993634_4994885_-|RNA-polymerase-nonessential-primary-like-sigma-factor	gnl|CDD|236067	PRK07598, PRK07598, RNA polymerase sigma factor SigC; Validated.	0
CP020114.1|AVZ31572.1|4971626_4973465_-|serine/threonine-protein-kinase-B	gnl|CDD|270916	cd14014, STKc_PknB_like, Catalytic domain of bacterial Serine/Threonine kinases, PknB and similar proteins. STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. This subfamily includes many bacterial eukaryotic-type STKs including Staphylococcus aureus PknB (also called PrkC or Stk1), Bacillus subtilis PrkC, and Mycobacterium tuberculosis Pkn proteins (PknB, PknD, PknE, PknF, PknL, and PknH), among others. S. aureus PknB is the only eukaryotic-type STK present in this species, although many microorganisms encode for several such proteins. It is important for the survival and pathogenesis of S. aureus as it is involved in the regulation of purine and pyrimidine biosynthesis, cell wall metabolism, autolysis, virulence, and antibiotic resistance. M. tuberculosis PknB is essential for growth and it acts on diverse substrates including proteins involved in peptidoglycan synthesis, cell division, transcription, stress responses, and metabolic regulation. B. subtilis PrkC is located at the inner membrane of endospores and functions to trigger spore germination. Bacterial STKs in this subfamily show varied domain architectures. The well-characterized members such as S. aureus and M. tuberculosis PknB, and B. subtilis PrkC, contain an N-terminal cytosolic kinase domain, a transmembrane (TM) segment, and mutliple C-terminal extracellular PASTA domains. The PknB subfamily is part of a larger superfamily that includes the catalytic domains of other protein STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase.	1.97937e-43
CP020114.1|AVZ31575.1|4982369_4983881_-|15-cis-phytoene-desaturase	gnl|CDD|225885	COG3349, COG3349, Uncharacterized conserved protein [Function unknown].	6.23588e-155
CP020114.1|AVZ31570.1|4959390_4962063_-|hypothetical-protein	gnl|CDD|379818	pfam16313, DUF4953, Met-zincin. This is a family of uncharacterized proteins that carry the highly characteristic met-zincin mmotif HExxHxxGxxH, the extended zinc-binding domain of metallopeptidases.	6.44901e-87

Protein sequences

>CP020114.1|AVZ31572.1|4971626_4973465_-|serine/threonine-protein-kinase-B
MTYCINPLCEARENPDNAEKCLACGTSLVIHGRIRLIKPLRELTEDPYVRIEVFEVDDSGTKWDTVRRLRIMKVLKWGNSKLVELFERESRTLRRLNHPCIPGSTIDDFFTFVPNNSPLTLRCLVMDKIEGQNLQKWIQSNGRISESLAVKWLKHLVEILDVVHHTEFFHRDIKPANIILQPDGRLALVDFGAARRITDTYLAKVSASGGTSTRMGNYEITAIVSPGYTPLEQINGKAVPQSDFYALGRTLVYLITAIPLVKLPTDENTGQLIWRNKAPQIGKRFADLIDEMMAPFPGQRPQTNEIILHRLKKLPSQTNFDKFVKSKTFILIVIGLVSFTGLGMFNMLLPVIANYFVSQGQNLETKKQSETAQKFFNWAIKINPRMNQRISSFYFEKAGRNFRNLQLAKTYYEKAIQYNPQDIDIYRNLALVCQYLNQVECVINNYKKALQIRGNDWELHYELGNFYDQANKYELAEKQYKIAVKLNKEAAAPINNLSRLKNREGNYYESEALARQGLNLTEKPEIKTTLYKNLGWAKLGQQQYIEADKYLQKSMEIDATKTDTYCLLAQVQDAVDETTNARILWEVCMIAESSLPEVLIWRQQLLDRLSKN
>CP020114.1|AVZ31571.1|4970102_4971116_-|hypothetical-protein
MTTARAIDKIIIALLLVLSSSSSVCGQQQRFAKRVCDDPVGRVINGGDLHLSVGNLICPEDQIQPANGFKVKIICYASRRILEIQQSKNAVDECKTEVPQSRDICIPITPGYCSNTRGTGETENKPQLITPYVGRILNTRPRLSWVNVPEATSYTIQVNGMGVNWSKNVQNTSLPYPSSLPAMQFGRIYQITIIANQGDKPVSANSFVTIVLPELEAQGIKSVIEQIESLSLDEDALAIDLDAVYMSKNLLTEAIGVLAARTKNGTQNPAIYRMLGDRYLETGLLETNSLAAAKRSYAEAVKLARQKNDAEELAKAQAGIKLLESQSQAPTRTNAAQ
>CP020114.1|AVZ31570.1|4959390_4962063_-|hypothetical-protein
MKNWITKLVICIILVYNLLHNFNYAAASQLSSTDVQQLTLLPEVENFGKNRKEDWPRFREIIKATDKLAGLFTLYSSQESGQIYWEITPEQLNKNYLATVTLESGIGESGLYSGLPLSDFLFYWRRVNNNLHFVVRNVKFRAEANQPEQRSLARSFSDSVLYSLPIESIDSYSKNILINLDNLLMQDFPGLTPLLKYSLQADYRLEQSKSYFGAVNSFPENIEIDAIYGFSSPDGANLITLPDSRGLTLQVHYSFSQLPENNGYIPRLADDRVGYFITAFQNFADHKDYEPFVRYINRWHLEPSDPDAAISPPKKPIVFWIENAVPSSYRDAIREGILMWNKAFAKAGFQNAIEVRQMPDDADWQPADIRYNTIRWFNSLDAGFARGPVRVNPFTGEILDADIIVDANMLRSIQHEYRALMATNSSLGKNQCPGNLPTMGSLDRTGLSDDVCYGMESSEQAAMGALALSILQKTTPSSEAMQDYVHQYLRSLIAHEVGHTLGLRHNFHGSTMLAPEDLNNPEITHSKGLVGSVMDYLPVNIAPPGIVQGDYFPGMVGPYDEWAIVYGYKTNVHPEAEKSFLEEIALASPQAELSYATDEDIWDINPLANLWDMSSDVLLYSQGQMDNARVMWQRLDKGYLSPGESYSHLRVLFNQILKYYFRNANLLPRYIGGQSFQRLHVNDYPNWAFVPVSLLKQRQVLTKLQEYVFAEDVFNFSPQLLNQLAPSRWQHWGNSVPYNRLDYPIHETIIRFQSEILRSLLDGDRLNRLRDIELKSPPGEALSIPELFDTLQQGIWTEVLAPEEPQTISSVRRSLQQEHLNILLEIILHPTDTPEDGRTIAWYKLCQLRKDIDISLKEFGEKLDIYTIAHLQFASDRITKTLNAELFSHQ
>CP020114.1|AVZ31569.1|4952001_4952685_-|3-oxoacyl-[acyl-carrier-protein]-reductase-FabG
MTKLILITGISKGLGYAMTERFIQEGHTFIGCARSSAAIEKLSQKFTAPNDFTVVDVKKEPQVEKWAKDVLSKYESPDIVINNAAITNYPAPLWQVPADEFSELIDINIKGVFNIIHHFVPAMVKNKRGIIVNFSSGWGRSTSPEVAPYCASKWAIEGLTRSLAQELPAGMAAVPLNPGIIHTDMLSISFGEEAVNYTPVSDWVLKAVPYILNLKPTDNGMPLTVSM
>CP020114.1|AVZ31568.1|4949309_4950242_-|hypothetical-protein
MLPSFYQPILSKYLSRTQLITLKMLVWLLQSQKQVKIERLAATIPLPIQQNSRRRHLQRFLKSNALSVVLLWFPIIEEILSRLIKPKSQLIIALDRTQWKDNNILMVSVIYQKRALPIYWCLLNKDGCSNLQEQQKVLRPVIRLLKHYQLVVIGDREFHGIELASWLHRQGLKYVFRQKKDTTFRKKRQDFQPLHTIPLSPGDRRFYPDVNLTQNKGFGRCNLAVYWKRKYRGKQEKEPWYLSTNLTDVSTTVKIYGQRFGIEAMFKDCKTGGYNLEGSQASPDRLVRLILLIAIAMTSAWLQGQKNSIF
>CP020114.1|AVZ31567.1|4948522_4949002_-|RNA-polymerase-nonessential-primary-like-sigma-factor
MPIHITEQLNKIKKVQRELFQKLGRPASITEIAETLNLQPSQIRDFLSTSRQPISLDVKVGDNQDTELNALLPDGGISPNEHITQELLRQDLNSLLASLKPTQREVLILRFGLENDQELTLAQIGEKMNVSRERVRQIQQQAITVLRRQQPEIEQYLFS
>CP020114.1|AVZ31566.1|4942746_4943178_-|hypothetical-protein
MIISDSHRQWEFKLEFVINRQPNREGYAIEYEDETQGLMIIETQLHGSRLAEGQRLIYVDGIASAPWNREMIQRPPNYKGVGTALLSFARTGSLELGYNGRVGLHSLPGSEKFYDLQGMIDVGEDEDYDDLIYFEYGIWRSST
>CP020114.1|AVZ31565.1|4942197_4942734_-|hypothetical-protein
MNQHEFNENQNNPGQHNQVNPMDLLLDEECLGQAVEAEELVGGNIGAGLNWGSAFDDLMLNFELMGRFTTLRISLNKEVRLLLNDWNLGTATKIAVKTARARILEKLRLPTAKVRQSLILALRNDELYGEEFISNKKILRELLELLLTPQDWETIAAVAADSLKQQIIHQAVGEKISA
>CP020114.1|AVZ31564.1|4941819_4942194_-|hypothetical-protein
MIAPTPEQQIFMQDDVSTRLHHLAEHLEQVQFLWTETSSQDLMMPLVKESRYFLEWTVPDMVKADNIDRAAELVDLGRVLTHWLFHWDNIWSDAEQKRVAQGQTQDWLHRVSEIADTEPEALSA
>CP020114.1|AVZ31563.1|4936385_4936727_-|putative-transcriptional-regulator
MAVLITLKKIREAQGISQNDLARITGYSVQNIQKIEQGRVSSLTLDAFDRFCKALGCLPGDLLVYTPDDDGSKETISQEQTKANITVNAKQTTNFSGRKKSVGLVLKFEQKTA
>CP020114.1|AVZ31573.1|4980781_4981093_-|hypothetical-protein
MVKIHAPIHIQIYEREGTQWFFHGGNIFHNPHGISTDLESTLERNGLTKIKVLVELFRVNGGKAGLYLADIRDKKYYYCGQKWEDVKGLLRELGIGRDEPSSS
>CP020114.1|AVZ31574.1|4981301_4981772_-|putative-adenylyltransferase/sulfurtransferase-MoeZ
MPSSFALNLCKFLIRLKFPHIHQISTKEFAQWLLNSTKIQPLVIDARSQAEYEVSHLKAAVHIDPIAPDLQILSQVAKETPIVVYCSIGYRSATISQQLQQAGFTRVFNLSGGLFQWANEGRLMFKNEEVTQCIHPYNAMWGKLLKDGKLRGRQRS
>CP020114.1|AVZ31575.1|4982369_4983881_-|15-cis-phytoene-desaturase
MTVGLQTKRVVVVGAGWAGLGATYHLAKQGYDVTLLEAGSYPGGLVAGWKTTAGKSVEAGIHGFWYPYRNIFSLINELKLNPFTTWTRSAQYSPAGLEVESPIFQDLPRLPAPLGTFLYTQFQRLPLIDRLSALPLLYSVIDFDNSHEAWRRYDFVTARELFKDFGVSARLYKEAFEPMLLVGLFAPGEQCSAAATLGMLYFFILAHQADFDVVWCRGTVGEKIFRPWVEKIAQAGAKVLPQRRVTDLIVDSQNRAKGVVCGDEVFDADAVIFAVGVTGMKKIVANSPSLQSREEFRNLSNLGAIDVLATRLWFDRKIDIPRPSNACFGFDATTGWTFFDLNALHDEYKNEPGTVIEADFYHANQFLNMSDADIITKVKNYLTTCVPAFGGAKVIDSSVIRLPNAVTHFAPGSYRHMLPAKTSFENVFMSGDWIVNRHGSWSQEKAYVTGLEAANLAVSYLGVGQPSEIIPVEEDEAHIQMGRSLNQSLSDLGKSVLPDFWLP
>CP020114.1|AVZ31576.1|4983959_4985816_-|two-component-system,-sensor-histidine-kinase-and-response-regulator
MLSSPDLSFSRNLPLVVFNQLRELLQQMAQVVGTTALVLTEAVLARIDTSLEWQTQRFTLVISEQFSALLLGTQEQGEEGNNTGFHASLTFNSQAIALFVSQVRDLFKGDSYTHQNLERYRQALRPNDAQIQTKFTLLLLEYLLPQPTEDVTEPLVYSCQPVEDALKKQIVQEQLLNQVTTQIRKSLDLPVIMGTTIALVREFLELDRLVVYKFAPSGVNTVNGENPLPGGGVVHEARATNAIPSVLYSQEEVCFNPNSPCWEKYRQGFTLVVDDVEKTYNLEACLLSFLRESQVRAKLVAPIMFEQKLWGLLIAHQCYTPRAWTKNEKNLLTSIAEQLAIAIHQADLMQSLQEATRTLIQEKQTLEQRVIERTMALREALLAAEAASRLRNEFLATISHELLTPLTYVIGMSSTLLRWPLGELSQRQRDYLQTIHDSGEHLLELINDILDLSQIEAGKTVLNITEFSLTDTAKSCLDSLFKKAQSKQINLKLDLQIDPQRDRFTADAGRVEQILWNLLTNAIKFTPEGGSVTLRIWVEDDSAIFQIEDTGIGIPEAQLPLMFEKFQQLDTPYRRRYEGTGLGLALTKQLVELHRGRIEVESTVAIGSIFTIWIPAQH
>CP020114.1|AVZ31577.1|4991480_4991936_-|ferric-uptake-regulation-protein
MTVYTSTSLKAELNDRGWRLTPQRETILHIFQELPQGEHLSAEDLYHRLESDGEGISLSTIYRTLKLMARMGILRELELGEGHKHYEINQPYPHHHHHLICVKCNSTIEFKNDSILKIGAKTAQKEGFHLLDCQMTIHAVCPKCQRALMPL
>CP020114.1|AVZ31578.1|4993634_4994885_-|RNA-polymerase-nonessential-primary-like-sigma-factor
MPATSFYADAASNTKKSRQAMEEELIIDDGELSADEIQELEVAAVDPANFAGNTNRRSTDLVRLYLQEIGRVRLLGRDEEVSEAQKVQRYLRMRTVLADAAKEGDEIVAPFLNLVQVQERLTSELGHRPSLERWANTAGIELVDLKPTLANGKRRWAEIAQLTVEELEQVQSQGLQAKEHMIKANLRLVVSVAKKYQNRGLELLDLVQEGTLGLERAVEKFDPTKGYRFSTYAYWWIRQGITRAIATSSRTIRLPVHITEKLNKIKKAQRKIAQEKGRTPTLEDLAIELEMTPTQVREVLLKVPRSVSLETKVGKDKDTELGELLETDCVTPEEMLMRESLQKDLHSLLADLTSRERDVILMRFGLSDGHPYSLAEIGRALDLSRERVRQIESKALQKLRQPKRRNLIRDYLESLS
>CP020114.1|AVZ31579.1|4995343_4996276_-|glycerol-3-phosphate-dehydrogenase
MIAVQKTTIAILGAGAWGIALADLASANGHQVRVWSRRRVANLETVIQGAEIILSAIAMKGVSDVVSQIQSFPLSPQTIFVTATKGLDPKTTCTPSQIWQTAFPHHPVVVLSGPNLSKEIQQSLPAATVVASHNTAAAEFVQLVFSSPRFRVYTNPDPLGVELGGTLKNIIAIAAGVCDGLHLGTNAKAALVTRGLTEMVRIGNSWGAKTETFYGLSGLGDLLATCNSPLSRNYQVGYQMTLGQTLPEILANLPGTAEGVNTCQVLVQRAKHHNIAVPITEQVYRLLQGEVTTRQALEELMLRDIKPEYK
>CP020114.1|AVZ31580.1|4996292_4997195_-|lipoyl-synthase
MTVKPDWLRVKAPQWERVGNVKEILRDLALNTVCEEASCPNIGECFQAGTATFLIMGPACTRACPYCDIDFEKKPQPLDPTEPARLAEAVRRMKLNHVVITSVNRDDLPDGGASQFVRCITAVRSVSPQTTIEVLIPDLCGNWNALEIILQAAPEVLNHNTETIERLYRRVRPQGNYQRTLELLKRSRQIAPWVYTKSGIMVGLGETDAEIRQAMQDLRSVDCDILTIGQYLQPSQKHLQVNDFITPEQFAAWQVFGEEIGFLQVVSSPLTRSSYHAEQVRELMQRYPRQAETQLIKSDL
>CP020114.1|AVZ31581.1|5003937_5004459_-|hypothetical-protein
MCHLKSKFGQYLRRCALIILIVLTVGLWGVESAQALLRQHHDSPGVLRYHSQVSIKDEKGYAWQVLLFKQNYNSPVKDLRLRVVGFPGIAEIAHPQPLEIEAASGKLLSASDVYALAAPAPNVGEYNLTNVLPQLSSTDALKLYVPTKSVKPLVLKIPNNVVTEWQWLVTEID
>CP020114.1|AVZ31582.1|5004800_5005010_-|hypothetical-protein
MITVLTLLLYLFITINVGRARAKYKVLPPQMTGNPDFERVVRVQQNTLEQMVFFLPALWLFCYIKPRQN

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity
CP020114_9	9.1|1986697|37|CP020114|CRISPRCasFinder	1986697-1986733	37	CP020114.1	970767-970803	1	0.973
CP020114_9	9.1|1986697|37|CP020114|CRISPRCasFinder	1986697-1986733	37	CP020114.1	970843-970879	1	0.973
CP020114_9	9.1|1986697|37|CP020114|CRISPRCasFinder	1986697-1986733	37	CP020114.1	970729-970765	2	0.946
CP020114_9	9.1|1986697|37|CP020114|CRISPRCasFinder	1986697-1986733	37	CP020114.1	970805-970841	2	0.946
CP020114_9	9.1|1986697|37|CP020114|CRISPRCasFinder	1986697-1986733	37	CP020114.1	1323736-1323772	2	0.946
CP020114_9	9.1|1986697|37|CP020114|CRISPRCasFinder	1986697-1986733	37	CP020114.1	3205368-3205404	2	0.946
CP020114_9	9.1|1986697|37|CP020114|CRISPRCasFinder	1986697-1986733	37	CP020114.1	3205406-3205442	2	0.946
CP020114_9	9.1|1986697|37|CP020114|CRISPRCasFinder	1986697-1986733	37	CP020114.1	3205444-3205480	2	0.946
CP020114_9	9.1|1986697|37|CP020114|CRISPRCasFinder	1986697-1986733	37	CP020114.1	3205482-3205518	2	0.946
CP020114_9	9.1|1986697|37|CP020114|CRISPRCasFinder	1986697-1986733	37	CP020114.1	3205520-3205556	2	0.946
CP020114_14	14.1|4154519|45|CP020114|CRISPRCasFinder	4154519-4154563	45	CP020114.1	1075330-1075374	1	0.978
CP020114_14	14.1|4154519|45|CP020114|CRISPRCasFinder	4154519-4154563	45	CP020114.1	5068473-5068517	1	0.978

1. spacer 9.1|1986697|37|CP020114|CRISPRCasFinder matches to position: 970767-970803, mismatch: 1, identity: 0.973

accctgcccccctgcctcttgtcccccctgcaccctg	CRISPR spacer
accctgcccccctgcctcttctcccccctgcaccctg	Protospacer
******************** ****************

2. spacer 9.1|1986697|37|CP020114|CRISPRCasFinder matches to position: 970843-970879, mismatch: 1, identity: 0.973

accctgcccccctgcctcttgtcccccctgcaccctg	CRISPR spacer
accctgcccccctgcctcttctcccccctgcaccctg	Protospacer
******************** ****************

3. spacer 9.1|1986697|37|CP020114|CRISPRCasFinder matches to position: 970729-970765, mismatch: 2, identity: 0.946

accctgcccccctgcctcttgtcccccctgcaccctg	CRISPR spacer
accctgcccccctgcctcttcccccccctgcaccctg	Protospacer
******************** .***************

4. spacer 9.1|1986697|37|CP020114|CRISPRCasFinder matches to position: 970805-970841, mismatch: 2, identity: 0.946

accctgcccccctgcctcttgtcccccctgcaccctg	CRISPR spacer
accctgcccccctgcctcttcccccccctgcaccctg	Protospacer
******************** .***************

5. spacer 9.1|1986697|37|CP020114|CRISPRCasFinder matches to position: 1323736-1323772, mismatch: 2, identity: 0.946

accctgcccccctgcctcttgtcccccctgcaccctg	CRISPR spacer
accctgctcccctgcctcttctcccccctgcaccctg	Protospacer
*******.************ ****************

6. spacer 9.1|1986697|37|CP020114|CRISPRCasFinder matches to position: 3205368-3205404, mismatch: 2, identity: 0.946

accctgcccccctgcctcttgtcccccctgcaccctg	CRISPR spacer
accctgcacccctgcctcttctcccccctgcaccctg	Protospacer
******* ************ ****************

7. spacer 9.1|1986697|37|CP020114|CRISPRCasFinder matches to position: 3205406-3205442, mismatch: 2, identity: 0.946

accctgcccccctgcctcttgtcccccctgcaccctg	CRISPR spacer
accctgcacccctgcctcttctcccccctgcaccctg	Protospacer
******* ************ ****************

8. spacer 9.1|1986697|37|CP020114|CRISPRCasFinder matches to position: 3205444-3205480, mismatch: 2, identity: 0.946

accctgcccccctgcctcttgtcccccctgcaccctg	CRISPR spacer
accctgcacccctgcctcttctcccccctgcaccctg	Protospacer
******* ************ ****************

9. spacer 9.1|1986697|37|CP020114|CRISPRCasFinder matches to position: 3205482-3205518, mismatch: 2, identity: 0.946

accctgcccccctgcctcttgtcccccctgcaccctg	CRISPR spacer
accctgcacccctgcctcttctcccccctgcaccctg	Protospacer
******* ************ ****************

10. spacer 9.1|1986697|37|CP020114|CRISPRCasFinder matches to position: 3205520-3205556, mismatch: 2, identity: 0.946

accctgcccccctgcctcttgtcccccctgcaccctg	CRISPR spacer
accctgcacccctgcctcttctcccccctgcaccctg	Protospacer
******* ************ ****************

11. spacer 14.1|4154519|45|CP020114|CRISPRCasFinder matches to position: 1075330-1075374, mismatch: 1, identity: 0.978

ccccaacccctttccttgctttcgcgtagcgtctccctttgggag	CRISPR spacer
ccccaacccctctccttgctttcgcgtagcgtctccctttgggag	Protospacer
***********.*********************************

12. spacer 14.1|4154519|45|CP020114|CRISPRCasFinder matches to position: 5068473-5068517, mismatch: 1, identity: 0.978

ccccaacccctttccttgctttcgcgtagcgtctccctttgggag	CRISPR spacer
ccccaacccctctccttgctttcgcgtagcgtctccctttgggag	Protospacer
***********.*********************************

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity
CP020114_15	15.3|4980026|29|CP020114|CRT	4980026-4980054	29	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36478-36506	0	1.0
CP020114_15	15.3|4980026|29|CP020114|CRT	4980026-4980054	29	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36847-36875	0	1.0
CP020114_15	15.1|4979903|29|CP020114|CRT	4979903-4979931	29	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36601-36629	1	0.966
CP020114_15	15.3|4980026|29|CP020114|CRT	4980026-4980054	29	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36601-36629	1	0.966
CP020114_15	15.1|4979903|29|CP020114|CRT	4979903-4979931	29	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36478-36506	2	0.931
CP020114_15	15.1|4979903|29|CP020114|CRT	4979903-4979931	29	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36847-36875	2	0.931
CP020114_15	15.1|4979903|29|CP020114|CRT	4979903-4979931	29	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36355-36383	2	0.931
CP020114_15	15.1|4979903|29|CP020114|CRT	4979903-4979931	29	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36724-36752	2	0.931
CP020114_15	15.3|4980026|29|CP020114|CRT	4980026-4980054	29	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36355-36383	2	0.931
CP020114_15	15.3|4980026|29|CP020114|CRT	4980026-4980054	29	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36724-36752	2	0.931
CP020114_15	15.2|4979966|26|CP020114|CRT	4979966-4979991	26	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36418-36443	4	0.846
CP020114_15	15.2|4979966|26|CP020114|CRT	4979966-4979991	26	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36541-36566	4	0.846
CP020114_15	15.2|4979966|26|CP020114|CRT	4979966-4979991	26	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36664-36689	4	0.846
CP020114_15	15.2|4979966|26|CP020114|CRT	4979966-4979991	26	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36787-36812	4	0.846
CP020114_15	15.1|4979903|29|CP020114|CRT	4979903-4979931	29	NZ_CP029555	Chromobacterium sp. IIBBL 274-1 plasmid unnamed, complete sequence	5531-5559	7	0.759
CP020114_15	15.1|4979903|29|CP020114|CRT	4979903-4979931	29	NZ_CP029555	Chromobacterium sp. IIBBL 274-1 plasmid unnamed, complete sequence	64802-64830	7	0.759
CP020114_15	15.3|4980026|29|CP020114|CRT	4980026-4980054	29	NZ_CP039845	Acetobacter pasteurianus strain CICC 22518 plasmid pAP22518-1, complete sequence	282007-282035	8	0.724
CP020114_15	15.3|4980026|29|CP020114|CRT	4980026-4980054	29	NZ_CP016452	Sinorhizobium sp. RAC02 plasmid pBSY16_1, complete sequence	1809632-1809660	8	0.724
CP020114_5	5.5|952803|32|CP020114|PILER-CR,CRISPRCasFinder,CRT	952803-952834	32	NZ_CP014797	Salipiger profundus strain JLT2016 plasmid pTPRO1, complete sequence	208297-208328	9	0.719
CP020114_5	5.5|952803|32|CP020114|PILER-CR,CRISPRCasFinder,CRT	952803-952834	32	NZ_CP029448	Serratia marcescens strain CAV1761 plasmid pCAV1761-205, complete sequence	89771-89802	10	0.688
CP020114_5	5.5|952803|32|CP020114|PILER-CR,CRISPRCasFinder,CRT	952803-952834	32	NZ_CP011641	Serratia marcescens strain CAV1492 plasmid pCAV1492-199, complete sequence	25722-25753	10	0.688
CP020114_5	5.3|952663|32|CP020114|PILER-CR,CRISPRCasFinder,CRT	952663-952694	32	NZ_CP015942	Legionella pneumophila strain C9_S plasmid unnamed1, complete sequence	7452-7483	11	0.656

1. spacer 15.3|4980026|29|CP020114|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 0, identity: 1.0

aggagggggatgatctcaccattaactcg	CRISPR spacer
aggagggggatgatctcaccattaactcg	Protospacer
*****************************

2. spacer 15.3|4980026|29|CP020114|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 0, identity: 1.0

aggagggggatgatctcaccattaactcg	CRISPR spacer
aggagggggatgatctcaccattaactcg	Protospacer
*****************************

3. spacer 15.1|4979903|29|CP020114|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 1, identity: 0.966

gggagggggatgctctcaccattaactcg	CRISPR spacer
gggagggggatgatctcaccattaactcg	Protospacer
************ ****************

4. spacer 15.3|4980026|29|CP020114|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 1, identity: 0.966

aggagggggatgatctcaccattaactcg	CRISPR spacer
gggagggggatgatctcaccattaactcg	Protospacer
.****************************

5. spacer 15.1|4979903|29|CP020114|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 2, identity: 0.931

gggagggggatgctctcaccattaactcg	CRISPR spacer
aggagggggatgatctcaccattaactcg	Protospacer
.*********** ****************

6. spacer 15.1|4979903|29|CP020114|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 2, identity: 0.931

gggagggggatgctctcaccattaactcg	CRISPR spacer
aggagggggatgatctcaccattaactcg	Protospacer
.*********** ****************

7. spacer 15.1|4979903|29|CP020114|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 2, identity: 0.931

gggagggggatgctctcaccattaactcg	CRISPR spacer
gggagggggatgatctcaccattaacccg	Protospacer
************ *************.**

8. spacer 15.1|4979903|29|CP020114|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 2, identity: 0.931

gggagggggatgctctcaccattaactcg	CRISPR spacer
gggagggggatgatctcaccattaacccg	Protospacer
************ *************.**

9. spacer 15.3|4980026|29|CP020114|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 2, identity: 0.931

aggagggggatgatctcaccattaactcg	CRISPR spacer
gggagggggatgatctcaccattaacccg	Protospacer
.*************************.**

10. spacer 15.3|4980026|29|CP020114|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 2, identity: 0.931

aggagggggatgatctcaccattaactcg	CRISPR spacer
gggagggggatgatctcaccattaacccg	Protospacer
.*************************.**

11. spacer 15.2|4979966|26|CP020114|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 4, identity: 0.846

ccgaaggggatcgcgctaaagatata	CRISPR spacer
aagaaggggagcgcgcgaaagatata	Protospacer
  ******** ***** *********

12. spacer 15.2|4979966|26|CP020114|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 4, identity: 0.846

ccgaaggggatcgcgctaaagatata	CRISPR spacer
aagaaggggagcgcgcgaaagatata	Protospacer
  ******** ***** *********

13. spacer 15.2|4979966|26|CP020114|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 4, identity: 0.846

ccgaaggggatcgcgctaaagatata	CRISPR spacer
aagaaggggagcgcgcgaaagatata	Protospacer
  ******** ***** *********

14. spacer 15.2|4979966|26|CP020114|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 4, identity: 0.846

ccgaaggggatcgcgctaaagatata	CRISPR spacer
aagaaggggagcgcgcgaaagatata	Protospacer
  ******** ***** *********

15. spacer 15.1|4979903|29|CP020114|CRT matches to NZ_CP029555 (Chromobacterium sp. IIBBL 274-1 plasmid unnamed, complete sequence) position: , mismatch: 7, identity: 0.759

gggagggggatgctctcaccattaactcg	CRISPR spacer
tggatggtgatgctctcaccattatccat	Protospacer
 *** ** **************** *.  

16. spacer 15.1|4979903|29|CP020114|CRT matches to NZ_CP029555 (Chromobacterium sp. IIBBL 274-1 plasmid unnamed, complete sequence) position: , mismatch: 7, identity: 0.759

gggagggggatgctctcaccattaactcg	CRISPR spacer
tggatggtgatgctctcaccattatccat	Protospacer
 *** ** **************** *.  

17. spacer 15.3|4980026|29|CP020114|CRT matches to NZ_CP039845 (Acetobacter pasteurianus strain CICC 22518 plasmid pAP22518-1, complete sequence) position: , mismatch: 8, identity: 0.724

aggagggggatgatctcaccattaactcg	CRISPR spacer
tagaggggggtgatctcaccataaatgga	Protospacer
 .*******.************ **.  .

18. spacer 15.3|4980026|29|CP020114|CRT matches to NZ_CP016452 (Sinorhizobium sp. RAC02 plasmid pBSY16_1, complete sequence) position: , mismatch: 8, identity: 0.724

aggagggggatgatctcaccattaactcg	CRISPR spacer
tcgaggcggatgatctcaccattcagcat	Protospacer
  **** **************** * .  

19. spacer 5.5|952803|32|CP020114|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP014797 (Salipiger profundus strain JLT2016 plasmid pTPRO1, complete sequence) position: , mismatch: 9, identity: 0.719

ttgctatggcgtttgattttgttccgaaaagc	CRISPR spacer
tgcttatggtgtttgattttgtttcgatgccc	Protospacer
*  .*****.*************.*** .  *

20. spacer 5.5|952803|32|CP020114|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP029448 (Serratia marcescens strain CAV1761 plasmid pCAV1761-205, complete sequence) position: , mismatch: 10, identity: 0.688

ttgctatggcgtttgattttgttccgaaaagc	CRISPR spacer
cacctatggcgtttgattatgttctgaggttt	Protospacer
.  *************** *****.**..  .

21. spacer 5.5|952803|32|CP020114|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP011641 (Serratia marcescens strain CAV1492 plasmid pCAV1492-199, complete sequence) position: , mismatch: 10, identity: 0.688

ttgctatggcgtttgattttgttccgaaaagc	CRISPR spacer
cacctatggcgtttgattatgttctgaggttt	Protospacer
.  *************** *****.**..  .

22. spacer 5.3|952663|32|CP020114|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP015942 (Legionella pneumophila strain C9_S plasmid unnamed1, complete sequence) position: , mismatch: 11, identity: 0.656

ccgataagcgaaaaacacgcttaagggtaatc	CRISPR spacer
aaattaagcgaaaaaagcgcttaagggattgt	Protospacer
  . *********** .**********    .

2. CP020115

CRISPR-Cas detection and classification

Crispr_ID: CP020115_1

• CRISPR_ID: CP020115_1
• CRISPR_location: 36321-36903
• CRISPR_type: Unclear
• Repeat_type: NA
• Spacer_info: 9 spacers
• Cas_protein_info: Cas14u_CAS-V,RT

Consensus repeat of CP020115_1

Consensus_repeat	Method
GAAATTTCCCTTAAGAATGAGAATAGTG	CRT

spacers of CP020115_1

>1.1|36349|35|CP020115|CRT
ATTGTCGGGAGGGGGATGATCTCACCATTAACCCG
>1.2|36412|32|CP020115|CRT
GTTTTCAAGAAGGGGAGCGCGCGAAAGATATA
>1.3|36472|35|CP020115|CRT
ATTGTCAGGAGGGGGATGATCTCACCATTAACTCG
>1.4|36535|32|CP020115|CRT
GTTTTCAAGAAGGGGAGCGCGCGAAAGATATA
>1.5|36595|35|CP020115|CRT
ATTGTCGGGAGGGGGATGATCTCACCATTAACTCG
>1.6|36658|32|CP020115|CRT
GTTTTCAAGAAGGGGAGCGCGCGAAAGATATA
>1.7|36718|35|CP020115|CRT
ATTGTCGGGAGGGGGATGATCTCACCATTAACCCG
>1.8|36781|32|CP020115|CRT
GTTTTCAAGAAGGGGAGCGCGCGAAAGATATA
>1.9|36841|35|CP020115|CRT
ATTGTCAGGAGGGGGATGATCTCACCATTAACTCG

CRISPR arrays and Neighbor proteins around CP020115_1

CRISPR arrays

The CRISPR arrays of CP020115_1

>merge|CP020115|1|36321-36903|CRT
GGAGTTTCCCTTAAGAATGAGAATAGTGATTGTCGGGAGGGGGATGATCTCACCATTAACCCGGAAATTTCCCTTAAGAATGAGAATAGTGGTTTTCAAGAAGGGGAGCGCGCGAAAGATATAGAAATTTCCCTTAAGAATGAGAATAGTGATTGTCAGGAGGGGGATGATCTCACCATTAACTCGGAAATTTCCCTTAAGAATGAGAATAGTGGTTTTCAAGAAGGGGAGCGCGCGAAAGATATAGAAATTTCCCTTAAGAATGAGAATAGTGATTGTCGGGAGGGGGATGATCTCACCATTAACTCGGAAATTTCCCTTAAGAATGAGAATAGTGGTTTTCAAGAAGGGGAGCGCGCGAAAGATATAGAAATTTCCCTTAAGAATGAGAATAGTGATTGTCGGGAGGGGGATGATCTCACCATTAACCCGGAAATTTCCCTTAAGAATGAGAATAGTGGTTTTCAAGAAGGGGAGCGCGCGAAAGATATAGAAATTTCCCTTAAGAATGAGAATAGTGATTGTCAGGAGGGGGATGATCTCACCATTAACTCGGAAATTTCCCTTAAGAATGAGAATAGTG

>CP020115|1|1|36321-36903|CRT
GGAGTTTCCCTTAAGAATGAGAATAGTG	ATTGTCGGGAGGGGGATGATCTCACCATTAACCCG
GAAATTTCCCTTAAGAATGAGAATAGTG	GTTTTCAAGAAGGGGAGCGCGCGAAAGATATA
GAAATTTCCCTTAAGAATGAGAATAGTG	ATTGTCAGGAGGGGGATGATCTCACCATTAACTCG
GAAATTTCCCTTAAGAATGAGAATAGTG	GTTTTCAAGAAGGGGAGCGCGCGAAAGATATA
GAAATTTCCCTTAAGAATGAGAATAGTG	ATTGTCGGGAGGGGGATGATCTCACCATTAACTCG
GAAATTTCCCTTAAGAATGAGAATAGTG	GTTTTCAAGAAGGGGAGCGCGCGAAAGATATA
GAAATTTCCCTTAAGAATGAGAATAGTG	ATTGTCGGGAGGGGGATGATCTCACCATTAACCCG
GAAATTTCCCTTAAGAATGAGAATAGTG	GTTTTCAAGAAGGGGAGCGCGCGAAAGATATA
GAAATTTCCCTTAAGAATGAGAATAGTG	ATTGTCAGGAGGGGGATGATCTCACCATTAACTCG
GAAATTTCCCTTAAGAATGAGAATAGTG

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
CP020115.1|AVZ31720.1|37602_37914_-|hypothetical-protein	unknown	unknown	unknown
CP020115.1|AVZ31718.1|25511_26531_-|hypothetical-protein	unknown	unknown	gnl|CDD|274350
CP020115.1|AVZ31725.1|49907_51290_-|hypothetical-protein	unknown	unknown	gnl|CDD|376573
CP020115.1|AVZ31719.1|26563_28411_-|serine/threonine-protein-kinase-B	unknown	unknown	gnl|CDD|270916
CP020115.1|AVZ31721.1|39111_39957_-|tyrosine-recombinase-XerD	unknown	unknown	gnl|CDD|234698
CP020115.1|AVZ31724.1|45486_45705_-|hypothetical-protein	unknown	unknown	unknown
CP020115.1|AVZ31712.1|5244_6441_-|hypothetical-protein	unknown	unknown	gnl|CDD|376573
CP020115.1|AVZ31728.1|56753_57992_-|hypothetical-protein	unknown	unknown	gnl|CDD|212669
CP020115.1|AVZ31717.1|18148_18523_-|putative-transposase	Cas14u_CAS-V	-	gnl|CDD|284650
CP020115.1|AVZ31729.1|58007_58517_-|hypothetical-protein	unknown	unknown	unknown
CP020115.1|AVZ31713.1|9440_10625_-|inosine-5'-monophosphate-dehydrogenase	unknown	unknown	gnl|CDD|341410
CP020115.1|AVZ31711.1|3393_3597_-|hypothetical-protein	unknown	unknown	unknown
CP020115.1|AVZ31715.1|15395_16424_-|hypothetical-protein	unknown	unknown	gnl|CDD|379252
CP020115.1|AVZ31727.1|54267_56463_-|ABC-transporter-ATP-binding/permease-protein	unknown	unknown	gnl|CDD|224054
CP020115.1|AVZ31714.1|10868_11210_-|hypothetical-protein	unknown	unknown	gnl|CDD|377856
CP020115.1|AVZ31723.1|44231_44627_-|hypothetical-protein	unknown	unknown	gnl|CDD|369598
CP020115.1|AVZ31716.1|17576_18140_-|3-oxoacyl-[acyl-carrier-protein]-reductase-FabG	unknown	unknown	gnl|CDD|212491
CP020115.1|AVZ31726.1|53423_54116_-|hypothetical-protein	unknown	unknown	gnl|CDD|378452
CP020115.1|AVZ31710.1|1823_2597_-|hypothetical-protein	unknown	unknown	unknown
CP020115.1|AVZ31722.1|42294_43500_-|group-II-intron-encoded-protein-LtrA	RT	pfam00078_RT_CAS-I:CAS-III	gnl|CDD|275209

HMMScan for Signature genes

Protein	Cas_name	Cas_description	E-value	Identity	Coverage
CP020115.1|AVZ31717.1|18148_18523_-|putative-transposase	Cas14u_CAS-V	-	2.5e-19	64.5	0.2
CP020115.1|AVZ31722.1|42294_43500_-|group-II-intron-encoded-protein-LtrA	RT	pfam00078_RT_CAS-I:CAS-III	4.8e-33	109.3	0.1

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
CP020115.1|AVZ31718.1|25511_26531_-|hypothetical-protein	gnl|CDD|274350	TIGR02917, TPR_domain_protein, putative PEP-CTERM system TPR-repeat lipoprotein. This protein family occurs in strictly within a subset of Gram-negative bacterial species with the proposed PEP-CTERM/exosortase system, analogous to the LPXTG/sortase system common in Gram-positive bacteria. This protein occurs in a species if and only if a transmembrane histidine kinase (TIGR02916) and a DNA-binding response regulator (TIGR02915) also occur. The present of tetratricopeptide repeats (TPR) suggests protein-protein interaction, possibly for the regulation of PEP-CTERM protein expression, since many PEP-CTERM proteins in these genomes are preceded by a proposed DNA binding site for the response regulator.	0.000728892
CP020115.1|AVZ31725.1|49907_51290_-|hypothetical-protein	gnl|CDD|376573	pfam01609, DDE_Tnp_1, Transposase DDE domain. Transposase proteins are necessary for efficient DNA transposition. This domain is a member of the DDE superfamily, which contain three carboxylate residues that are believed to be responsible for coordinating metal ions needed for catalysis. The catalytic activity of this enzyme involves DNA cleavage at a specific site followed by a strand transfer reaction. This family contains transposases for IS4, IS421, IS5377, IS427, IS402, IS1355, IS5, which was original isolated in bacteriophage lambda.	2.57981e-11
CP020115.1|AVZ31719.1|26563_28411_-|serine/threonine-protein-kinase-B	gnl|CDD|270916	cd14014, STKc_PknB_like, Catalytic domain of bacterial Serine/Threonine kinases, PknB and similar proteins. STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. This subfamily includes many bacterial eukaryotic-type STKs including Staphylococcus aureus PknB (also called PrkC or Stk1), Bacillus subtilis PrkC, and Mycobacterium tuberculosis Pkn proteins (PknB, PknD, PknE, PknF, PknL, and PknH), among others. S. aureus PknB is the only eukaryotic-type STK present in this species, although many microorganisms encode for several such proteins. It is important for the survival and pathogenesis of S. aureus as it is involved in the regulation of purine and pyrimidine biosynthesis, cell wall metabolism, autolysis, virulence, and antibiotic resistance. M. tuberculosis PknB is essential for growth and it acts on diverse substrates including proteins involved in peptidoglycan synthesis, cell division, transcription, stress responses, and metabolic regulation. B. subtilis PrkC is located at the inner membrane of endospores and functions to trigger spore germination. Bacterial STKs in this subfamily show varied domain architectures. The well-characterized members such as S. aureus and M. tuberculosis PknB, and B. subtilis PrkC, contain an N-terminal cytosolic kinase domain, a transmembrane (TM) segment, and mutliple C-terminal extracellular PASTA domains. The PknB subfamily is part of a larger superfamily that includes the catalytic domains of other protein STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase.	2.80642e-42
CP020115.1|AVZ31721.1|39111_39957_-|tyrosine-recombinase-XerD	gnl|CDD|234698	PRK00236, xerC, site-specific tyrosine recombinase XerC; Reviewed.	6.7904e-56
CP020115.1|AVZ31723.1|44231_44627_-|hypothetical-protein	gnl|CDD|369598	pfam07924, NuiA, Nuclease A inhibitor-like protein. This family consists of protein sequences that are similar to the nuclease A inhibitor expressed by bacteria of the genus Anabaena ((NuiA). This sequence is organized to form an alpha-beta-alpha sandwich fold, which is similar to the PR-1-like fold. NuiA interacts with nuclease A by means of residues located at one end of the molecule, including residues making up the loop between helices III and IV and the loop between strands C and D. The mechanism of inhibition of nuclease A by NuiA is as yet incompletely understood.	2.28653e-62
CP020115.1|AVZ31728.1|56753_57992_-|hypothetical-protein	gnl|CDD|212669	cd10227, ParM_like, Plasmid segregation protein ParM and similar proteins. ParM is a plasmid-encoded bacterial homolog of actin, which polymerizes into filaments similar to F-actin, and plays a vital role in plasmid segregation. ParM filaments segregate plasmids paired at midcell into the individual daughter cells. This subfamily also contains Thermoplasma acidophilum Ta0583, an active ATPase at physiological temperatures, which has a propensity to form filaments.	1.93626e-20
CP020115.1|AVZ31717.1|18148_18523_-|putative-transposase	gnl|CDD|284650	pfam07282, OrfB_Zn_ribbon, Putative transposase DNA-binding domain. This putative domain is found at the C-terminus of a large number of transposase proteins. This domain contains four conserved cysteines suggestive of a zinc binding domain. Given the need for transposases to bind DNA as well as the large number of DNA-binding zinc fingers we hypothesize this domain is DNA-binding.	8.7676e-16
CP020115.1|AVZ31713.1|9440_10625_-|inosine-5'-monophosphate-dehydrogenase	gnl|CDD|341410	cd17774, CBS_two-component_sensor_histidine_kinase_repeat2, 2 tandem repeats of the CBS domain in the two-component sensor histidine kinase and related-proteins, repeat 2. This cd contains 2 tandem repeats of the CBS domain in the two-component sensor histidine kinase and related-proteins. Two-component regulation is the predominant form of signal recognition and response coupling mechanism used by bacteria to sense and respond to diverse environmental stresses and cues ranging from common environmental stimuli to host signals recognized by pathogens and bacterial cell-cell communication signals. The structures of both sensors and regulators are modular, and numerous variations in domain architecture and composition have evolved to tailor to specific needs in signal perception and signal transduction. The simplest histidine kinase sensors consists of only sensing and kinase domains. The more complex hybrid sensors contain an additional REC domain typical of two-component regulators and in some cases a C-terminal histidine phosphotransferase (HPT) domain. The CBS domain, named after human CBS, is a small domain originally identified in cystathionine beta-synthase and is subsequently found in a wide range of different proteins. CBS domains usually occur in tandem repeats. They associate to form a so-called Bateman domain or a CBS pair based on crystallographic studies in bacteria. The CBS pair was used as a basis for this cd hierarchy since the human CBS proteins can adopt the typical core structure and form an intramolecular CBS pair. The interface between the two CBS domains forms a cleft that is a potential ligand binding site. The CBS pair coexists with a variety of other functional domains and this has been used to help in its classification here. It has been proposed that the CBS domain may play a regulatory role, although its exact function is unknown. Mutations of conserved residues within this domain are associated with a variety of human hereditary diseases, including congenital myotonia, idiopathic generalized epilepsy, hypercalciuric nephrolithiasis, and classic Bartter syndrome (CLC chloride channel family members), Wolff-Parkinson-White syndrome (gamma 2 subunit of AMP-activated protein kinase), retinitis pigmentosa (IMP dehydrogenase-1), and homocystinuria (cystathionine beta-synthase).	3.90022e-78
CP020115.1|AVZ31715.1|15395_16424_-|hypothetical-protein	gnl|CDD|379252	pfam13546, DDE_5, DDE superfamily endonuclease. This family of proteins are related to pfam00665 and are probably endonucleases of the DDE superfamily. Transposase proteins are necessary for efficient DNA transposition. This domain is a member of the DDE superfamily, which contain three carboxylate residues that are believed to be responsible for coordinating metal ions needed for catalysis. The catalytic activity of this enzyme involves DNA cleavage at a specific site followed by a strand transfer reaction.	0.000432813
CP020115.1|AVZ31727.1|54267_56463_-|ABC-transporter-ATP-binding/permease-protein	gnl|CDD|224054	COG1131, CcmA, ABC-type multidrug transport system, ATPase component [Defense mechanisms].	2.12337e-62
CP020115.1|AVZ31714.1|10868_11210_-|hypothetical-protein	gnl|CDD|377856	pfam07508, Recombinase, Recombinase. This domain is usually found associated with pfam00239 in putative integrases/recombinases of mobile genetic elements of diverse bacteria and phages.	2.70739e-07
CP020115.1|AVZ31712.1|5244_6441_-|hypothetical-protein	gnl|CDD|376573	pfam01609, DDE_Tnp_1, Transposase DDE domain. Transposase proteins are necessary for efficient DNA transposition. This domain is a member of the DDE superfamily, which contain three carboxylate residues that are believed to be responsible for coordinating metal ions needed for catalysis. The catalytic activity of this enzyme involves DNA cleavage at a specific site followed by a strand transfer reaction. This family contains transposases for IS4, IS421, IS5377, IS427, IS402, IS1355, IS5, which was original isolated in bacteriophage lambda.	1.42266e-12
CP020115.1|AVZ31716.1|17576_18140_-|3-oxoacyl-[acyl-carrier-protein]-reductase-FabG	gnl|CDD|212491	cd05233, SDR_c, classical (c) SDRs. SDRs are a functionally diverse family of oxidoreductases that have a single domain with a structurally conserved Rossmann fold (alpha/beta folding pattern with a central beta-sheet), an NAD(P)(H)-binding region, and a structurally diverse C-terminal region. Classical SDRs are typically about 250 residues long, while extended SDRs are approximately 350 residues. Sequence identity between different SDR enzymes are typically in the 15-30% range, but the enzymes share the Rossmann fold NAD-binding motif and characteristic NAD-binding and catalytic sequence patterns. These enzymes catalyze a wide range of activities including the metabolism of steroids, cofactors, carbohydrates, lipids, aromatic compounds, and amino acids, and act in redox sensing. Classical SDRs have an TGXXX[AG]XG cofactor binding motif and a YXXXK active site motif, with the Tyr residue of the active site motif serving as a critical catalytic residue (Tyr-151, human prostaglandin dehydrogenase (PGDH) numbering). In addition to the Tyr and Lys, there is often an upstream Ser (Ser-138, PGDH numbering) and/or an Asn (Asn-107, PGDH numbering) contributing to the active site; while substrate binding is in the C-terminal region, which determines specificity. The standard reaction mechanism is a 4-pro-S hydride transfer and proton relay involving the conserved Tyr and Lys, a water molecule stabilized by Asn, and nicotinamide. Extended SDRs have additional elements in the C-terminal region, and typically have a TGXXGXXG cofactor binding motif. Complex (multidomain) SDRs such as ketoreductase domains of fatty acid synthase have a GGXGXXG NAD(P)-binding motif and an altered active site motif (YXXXN). Fungal type ketoacyl reductases have a TGXXXGX(1-2)G NAD(P)-binding motif. Some atypical SDRs have lost catalytic activity and/or have an unusual NAD(P)-binding motif and missing or unusual active site residues. Reactions catalyzed within the SDR family include isomerization, decarboxylation, epimerization, C=N bond reduction, dehydratase activity, dehalogenation, Enoyl-CoA reduction, and carbonyl-alcohol oxidoreduction.	1.97423e-40
CP020115.1|AVZ31726.1|53423_54116_-|hypothetical-protein	gnl|CDD|378452	pfam10592, AIPR, AIPR protein. This family of proteins was identified in as an abortive infection phage resistance protein often found in restriction modification system operons.	1.41319e-29
CP020115.1|AVZ31722.1|42294_43500_-|group-II-intron-encoded-protein-LtrA	gnl|CDD|275209	TIGR04416, hypothetical_protein, group II intron reverse transcriptase/maturase. Members of this protein family are multifunctional proteins encoded in most examples of bacterial group II introns. These group II introns are mobile selfish genetic elements, often with multiple highly identical copies per genome. Member proteins have an N-terminal reverse transcriptase (RNA-directed DNA polymerase) domain (pfam00078) followed by an RNA-binding maturase domain (pfam08388). Some members of this family may have an additional C-terminal DNA endonuclease domain that this model does not cover. A region of the group II intron ribozyme structure should be detectable nearby on the genome by Rfam model RF00029. [Mobile and extrachromosomal element functions, Other].	9.78754e-80

Protein sequences

>CP020115.1|AVZ31719.1|26563_28411_-|serine/threonine-protein-kinase-B
MSYCINPLCDHRQNPDDAQNCLSCGTSLVINNRIRLIKPLRALNDNPFSYTEVFEVDDAGTQWNPVRKQRVMKVLKLNSPKLVELIERESLSLRLIHHPNIPKSTLDDFFTFVPNKSSLILHCLVMDKIKGQNLEKWIESNGRILQSQALEWLKQLVEILAAVHAANFFHRDIKPSNIILQSSGQLALVDFGVARRVTSTYLAKISGSGGDSTSRGGKYEITSVGTPRYSPPEQTDGQAVPQSDFYALGRTFVHLLTAIQLIDLPTDKQTGRLIWRNKAPQIDKPFADFIDELMAPFPGQRPQTTRVILQRLEKLPLQSKFHSFTKSKTFIISAIITSIALGSFGIFKIALPGLANALVSEGQNLEAENKSENAQQLFKWAIKIHPQSSNNISSFYFGKAGRNLTNLEVAKKYLEKAIKYNPQDANAYINLALVCQNLRQLECAIANQKKAISLHPSNGILYYDLGSIYDDFGKYKLAEEKYNIAIKLSQNPAAPINNLSRLKNIEEKYHEAEALALKGLKLTENPEIKTTLYKNLGWAKLGQRKYSEANNYLQKSRDIDATKTDTFCLLAQAQDALGEKTKARIYWEVCIIAESNLPEVFNWRQQLLDRLIEKL
>CP020115.1|AVZ31718.1|25511_26531_-|hypothetical-protein
MVNFMMFIPKVINNTFMALLLVISNSSSVWSQPPRLAKGVCHDPVGRVINGGDVHLSAGSLICPEDRIQPAKGSMVEVICLTSRRILEIKQSDSAAQVCAERVSQPRRLCILDANCRNTRGSEERENPTLIAPYVGRIINPRPKLSWLEVLGATSYIVQVEGSGGNWSKEIPKTSLEYPLSEPAMEWGRIYKINVIANKGDEPVSASSSVTIVLPQEEAQEINRVVEKIQNLNLSADELAIDLDAVYMSKNLLTEAIGVLEARVRNGTQNPAIYRMLGDRYLETGLLETHSLAAAIRAYAEAVKLARQKNDAEELAKAQAGVKLLESQSQAPTRTNAAQ
>CP020115.1|AVZ31717.1|18148_18523_-|putative-transposase
MVFVEDLNLVGLSCGMLGKHCLDAGFGQFFHILEQTCMKRDVYFQKVDSRKTSQICPNCGVETGKKELSERTHVCSNCGYTTDRDVAAAQVVLVRGLAAVGHTVKMLAEGKFIGIPVKQESSHL
>CP020115.1|AVZ31716.1|17576_18140_-|3-oxoacyl-[acyl-carrier-protein]-reductase-FabG
MSSQKFTAPNDFTVVDVKKEPQVEKWAKDVLSKYDPPDIVINNAAITNYPAPLWQVPSDEFSELIDINIKGVVNIIRHFVPAMLKNKRGIIVNFSSGWGRSTSPEVAPYCASKWAIEGLTRSLAQELPAGMAAVPLNPGIIHTDMLSISFGEEAVNYTPVSDWVLKAVPYILNLKPTDNGMPLTVSM
>CP020115.1|AVZ31715.1|15395_16424_-|hypothetical-protein
MLVISMRFTKLNYCQYLLSSQINYTITNLAEHLEHISHDAINYYLKREKLTPRLLWENVKDVIELDANSYIIFDDSILDKRYSKEIEMVRKQYSGNEHGIIKGIGVVSCVYVNPKVQRFWVIDYRIFNPDVDGKTKIDHVKDMLQNLVYQKLLPFDTVLMDTWYAVHSLMLYIDSLEKVYYCPLKNNRLVDDTLGKEKYKRIDLLQWSEEELECGKIIKIKGFPAQKKVKLFRVAVSTNRTDYVATNDLSQSSTNVVQKVCKIRWKIEEFHREIKQLTGIESCQCRKARLQRNHIACAMLVWIRLKNLAYQTGQTVYQIKHNLLSNYLIQQLKRPSISMCLV
>CP020115.1|AVZ31714.1|10868_11210_-|hypothetical-protein
MATEKSKDSYLWEKLDQARKAKAASGGYAGYGSPAFGQQSVNGELVENPSEQQIIELIRRHHKSGKSLPQIAAWLNQQGYTTKRGSQWQPVSVKRVLDRLYGKIQRISGMDED
>CP020115.1|AVZ31713.1|9440_10625_-|inosine-5'-monophosphate-dehydrogenase
MQIDDPLIGVPCLEEAIDRHPLVVAPHTSLINVVNLMNQTRGNSCLLPDFDSAIGFSSMGGTRSSCILVMEETEILGIFTERDIVKLTAAGRDFADVKIADVMVHPVITLAETAFRDIFAALFLFRRYRIRHLVIVNAEEQLVGIVSPESIRQVLRPTNLLKMRRVSEVMTTQVIHAPPTASVLSLAQMMAENQVSCVVIVKRDSWNDVLSAFKPVGIVTERDIVQFQALGLNLPQIEAKTVMSTPLFLLSPEDSLWSAHQEMQQRRVQRLVVYWDWGAKLAIVTQTSLLRIFDPLEMYGVIETLQLTVAQLETEKAKSFHNQSNSTELPLQPDLKQIQKQHIVAEENLDNLIATVESRIEHLIKNPNLSTELQQMYLGLATTEMQKIRHCLHS
>CP020115.1|AVZ31712.1|5244_6441_-|hypothetical-protein
MNTPIISKSVKILKQKFTHSLGLPIHQLLPSEVIQEELAQLNIKYRQRLFDPICTLWAFLSQVLDVDKSCHNAVSRVIAYLVGSGVELPSTDTSAYCQARQRLPEELIQKLFGKVAVGLESQVNETHLWCGRKVKVIDGSTVSMPDTPDNQNEYPQPSSQKIGCGFPLAKIGALFSIATGAAVALAIDVFNTHDIQLARRLYEFLSPGDVLLGDRAFCAYADIFQIKSLGGDAVFRKHQGRQTQMRRGKIVGSCDKIVTWSKPKKCPQGLSQEEFNSLPNTLIVREIYFYIFIPGFRTEQVSLITTLLDTKAYSTLDIMSLYEKRWDVEVDLKHLKTTLGMDILRSKTPAMVRKEIWAFLLAYNLLRTLMWSAGNSYGVSPLRLSLQGTRQHFDNFIP
>CP020115.1|AVZ31711.1|3393_3597_-|hypothetical-protein
MLLNLSFKASIADSSQENGGLPSLGLPTGTLTGLTQKPSQTLIPLCPLRLVWYASGTLRERYSVICA
>CP020115.1|AVZ31710.1|1823_2597_-|hypothetical-protein
MMVNKTSKAATNELVIKAKFFAFVSLIAFLPLPVRALTLIENRNALLSNDQIEWSSLGKLLNPFAPNFEDFLPNTFSAQSSNGLGLTVEIPSADGDITAPFVFQNNPPVQTNFGAGDFLLFTGFSPTVIPTGNSNPITIVFNEKIKAVGTQIAASGSENIDYEIFLSVFDEANNLLGTFSLPGISSGNRDNSAVFLGVKSDTVNIKSVVIRTSSPERGLGINTVSIVTVNEPYSGLSLLMIGSVVLFKSYQFKKCKK
>CP020115.1|AVZ31720.1|37602_37914_-|hypothetical-protein
MVKIHAPIHIQIYEREGTQWFFHGGNIFHNPHGISTDLESTLERNGLTKIKVLVELFRVNGGKAGLYLADIRDKKYYYCGQKWEDVKGLLRELGIGRDEPSSS
>CP020115.1|AVZ31721.1|39111_39957_-|tyrosine-recombinase-XerD
MLNHSSTLTNTELLDLWLHGKSKNTIDGYRRHAERFFCHVNKPLADCTLMDIQLWVMTLHCSDNSKRVAVGAIKSLLSFAKQLGVVSANLGILIKSPKSRNRLAERILTQEEVKLLISSTTNSRDHAIIRLFYLAGLRVSELCGLKWRDLKARGDGGQITVFGKGEKTRTVLVGAGMWKEINSLKGYAKHDDPVFISAKGGHLCRSMVFHIVKKAANRAGIEGNVSPHWLRHSHASHSLDRGAPIHLLQKTLGHSSVAITEMYLHARPTDSSGLYLPDDDE
>CP020115.1|AVZ31722.1|42294_43500_-|group-II-intron-encoded-protein-LtrA
MDADISKCFDKINHQALLKKINTFPTLNRLIKKWLKAGVFDNNEWLPTKQGTPQGGVISPLLANIALHGMEEEINKVADSLPGNKKKNRYALSFIRYADDFIIIHPSFDVVNKCKDRISEWLSEMGLELKPSKTKIVHTLKPEPNIGTQSSINTGGFDFLGFNVRQYQVGKTHSGKNSKGNLLGFKTLIKPSNKAIRKHYDEICKIIDTYKSAPQEALIKRLNPIIRGWTNYYSSVVSKEIFSSLDHIIFHKLQTWAKRRHPNKSKSWISSKYWGTFGNNNWVFSTKYEGKITFTLLKYVKTEIVRHIKVEGKKSPFDGNLVYWSERRGKSTLLPLRVTTLLKSQKGKCNHCGLHFREDDVMEVDHIIPKSKGGKNDYKNLQLLHRHCHDVKTAISGSRYA
>CP020115.1|AVZ31723.1|44231_44627_-|hypothetical-protein
MTKTNAEILEQLKQASEGLLFMSESEYPCDICLWSDATPEQVVHLTNHPQDTPVEVEGVDDFFSVATTAQDWHSEEEKETVHKFQTLVQILKANLSNLQVYRLGEVEVDVYVVGQTPTGDLAGIATKIVET
>CP020115.1|AVZ31724.1|45486_45705_-|hypothetical-protein
MELWQPEGASYYNGELIVQPQQRWTPEEQLEMLDTHPLFTGFCPQCGAEFDRDYVSRVHWDCNNCGWKDDSI
>CP020115.1|AVZ31725.1|49907_51290_-|hypothetical-protein
MNTPIISKSVKILKQKFTHSLGLPIHQLLPSEVIQEELAQLNIKYRQRLFDPICTLWAFLSQVLDVDKSCHNAVSRVIAYLVGSGVELPSTDTSAYCQARQRLPEELIQKLFGKVAVGLESQVNETHLWCGRKVKVIDGSTVSMPDTPDNQNEYPQPSSQKIGCGFPLAKIGALFSIATGAAVALAIDVFNTHDIQLARRLYEFLSPGDVLLGDRAFCAYADIFQIKSLGGDAVFRKHQGRQTQMRRGKIVGSCDKIVTWSKPKKCPQGLSQEEFNSLPNTLIVREIYFYIFIPGFRTEQVSLITTLLDTKAYSTLDITSLYEKRWDVEVDLKHLKTTLGMDILRSKTPAMVRKEIWAFLLAYNLLRTLMWSAGNSYGVSPLRLSLQGTRQHFDNFIPQFIAATNSDRHQIYSTLLKVIVHKEVPLRQARSEPRVRKRRPKSYPLMQELRQRYHDNLKTA
>CP020115.1|AVZ31726.1|53423_54116_-|hypothetical-protein
MPKTWILKIDNYFNANPNCIIATAHIDKFPTDLPLEPNIREPNRRSATYRQILDSLTIQPEKFFERHSGIVLCANKVKPNKTQTELELEILEASEGGSDGIINGGHTVLAFEQARNYKYDLSQAKVKVTIHIGLNEAEAKDIALASNTTSPVDSRSKVNARGDYEFIKQYLAELERTEDRKFRVAYYQNQTGAPRNPQCNGEQRDFVKLGQKVAIANHLFSVQSNFIYNI
>CP020115.1|AVZ31727.1|54267_56463_-|ABC-transporter-ATP-binding/permease-protein
MQTLQLTAHTNPIQQCTVTQLPFTIGSDPNNDLVVSDKDVDSYHAAILLGKNQYWLFDVGSSSGTRIGSTQLRHQQSQALSDGNLIQVGSIIIEIKITNPPTAKAHIFTPVAKTQLFNKKPENPILQNLRLDAINLYKSTSLFFGTTLINKISVSFLPQEFIVIAGVSGGGKSTLMDALNGQRRANSGKVMVNGVNLYRHFSAFKHSIGYVPQDDIVHPELTVREALHYAAQLRLPHATPEIRQQRIQAVLEQLHLTPRKHVPIKQLSGGQRKRVSIGVELITKPSLFFLDEATSGLDPGTELQIMELLRDLAHNGSTIVLITHATKNVAITDFVVFLAKGGRLAYFGPPKMAFDYFKVPDFDAIYNKVERQLSPEKWEERFKESVFHRKFISDRQESIPQTKVTPVAPRINYWQQWWVLCRRNLTILFKNKMSLSLMLTGAPILGGFNFLLWKSDLFDTKTGNPGQSITMLFVTVIMAVMAGALATMPEIAKETAIYRRERSAGLGVLPYLASKFHLSFLLALYQSAVLLGCMVSAVTVPESALPGMYVTLFLASFGSMVLGLLVSAIAPNQSVAPLLTILVLVPQVIFGGGILPVSEFKDSGKFLNQLMLTKYPFESLVTLSGLGEDVAKDPCWQKPERERRRLSVQESNACDCYGNNVFKQCQFPGLGTVDRSSFLSSDVFKARQLISHVHQTYGDIFSINVMLSLMQMTFLIGAIAILLFVFQSAKG
>CP020115.1|AVZ31728.1|56753_57992_-|hypothetical-protein
MTKNQDKSKNVTRLVLTCDLGGSQTKAIAQIYPDGIPIVLAMSPEVADVSQGSIEWLIQQNQFFHGNNAWVGLGEEYYVLGELAKTMFAGTAAIRDLKYQYALPKIMAMLWLACRNFNLNSFSEVFVQLLLPPGEVSDGEQLAIELAQALKKGVTTPTVRLKGKLRNFDVSREGSGIMAYYRRSIQNKYFQNNIGMLMLGYRNASFYLSQKGNPSLAESTQWGMNWVVAQFVERTAVGLSKYDSRLIPALLAAGKGDFDDLRALSRKTTESGIESDLKLFQKLLPDVRDEYCLAVLRWMRNIADLDEIVICGGTAEFVRQELTEHFENEGIPIIWNGNVSIPAKLDTLDLGDRVTDVWTTHISHIKMLDKNLGHDRQGRTLVPTDNQPTYYNGRVPEEVWIKQGFMPMHEGV
>CP020115.1|AVZ31729.1|58007_58517_-|hypothetical-protein
MTDAKHQRISIKRLDDSVDGQVISYLQSDPFDENESFPELVLSTLKAHWLPLTLLSKGVGGEKLRQAGIRAIAKLEAQISTIRRICGLDIEPVHVAPVVINQTFVSANDKTSTWGEISDSHSNNSSQSNAQTNEDEIDEDDDDWDVEAALMRMPKPEELRDANRMMGLG

Crispr_ID: CP020115_2

• CRISPR_ID: CP020115_2
• CRISPR_location: 45845-46095
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 4 spacers
• Cas_protein_info: RT,Cas14u_CAS-V

Consensus repeat of CP020115_2

Consensus_repeat	Method
TTTCCCTTAAGAATTTTCTGGGG	CRISPRCasFinder

spacers of CP020115_2

>2.1|45868|50|CP020115|CRISPRCasFinder
CTTGAGTGCGATGGGCAAAGCCCCGCCGTAGGCGATCGCCACTGATAACT
>2.2|45941|24|CP020115|CRISPRCasFinder
GCTACTCCTGCGGTGCGCTCATCA
>2.3|45988|25|CP020115|CRISPRCasFinder
TGCGAGTTCTGCGGTGCGCTCATCA
>2.4|46036|37|CP020115|CRISPRCasFinder
AGTGTGAGTGTTTGTACCTATGTCCCATGTCCCAACT

CRISPR arrays and Neighbor proteins around CP020115_2

CRISPR arrays

The CRISPR arrays of CP020115_2

>merge|CP020115|2|45845-46095|CRISPRCasFinder
TTTCCCTTAAGAATTTTCTGGGGCTTGAGTGCGATGGGCAAAGCCCCGCCGTAGGCGATCGCCACTGATAACTTTTCCCTTAAGAATTTTCTGGGGGCTACTCCTGCGGTGCGCTCATCATTTCCCTTAAGAATTTTCTGGGGTGCGAGTTCTGCGGTGCGCTCATCATTTCCCTTAAGAATTTTCTAGTGAGTGTGAGTGTTTGTACCTATGTCCCATGTCCCAACTTTTCCCTTAAGAATTTCCAAATA

>CP020115|2|1|45845-46095|CRISPRCasFinder
TTTCCCTTAAGAATTTTCTGGGG	CTTGAGTGCGATGGGCAAAGCCCCGCCGTAGGCGATCGCCACTGATAACT
TTTCCCTTAAGAATTTTCTGGGG	GCTACTCCTGCGGTGCGCTCATCA
TTTCCCTTAAGAATTTTCTGGGG	TGCGAGTTCTGCGGTGCGCTCATCA
TTTCCCTTAAGAATTTTCTAGTG	AGTGTGAGTGTTTGTACCTATGTCCCATGTCCCAACT
TTTCCCTTAAGAATTTCCAAATA

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
CP020115.1|AVZ31720.1|37602_37914_-|hypothetical-protein	unknown	unknown	unknown
CP020115.1|AVZ31730.1|61505_62069_-|hypothetical-protein	unknown	unknown	unknown
CP020115.1|AVZ31718.1|25511_26531_-|hypothetical-protein	unknown	unknown	gnl|CDD|274350
CP020115.1|AVZ31732.1|67381_68173_-|hypothetical-protein	unknown	unknown	unknown
CP020115.1|AVZ31724.1|45486_45705_-|hypothetical-protein	unknown	unknown	unknown
CP020115.1|AVZ31721.1|39111_39957_-|tyrosine-recombinase-XerD	unknown	unknown	gnl|CDD|234698
CP020115.1|AVZ31725.1|49907_51290_-|hypothetical-protein	unknown	unknown	gnl|CDD|376573
CP020115.1|AVZ31728.1|56753_57992_-|hypothetical-protein	unknown	unknown	gnl|CDD|212669
CP020115.1|AVZ31717.1|18148_18523_-|putative-transposase	Cas14u_CAS-V	-	gnl|CDD|284650
CP020115.1|AVZ31729.1|58007_58517_-|hypothetical-protein	unknown	unknown	unknown
CP020115.1|AVZ31715.1|15395_16424_-|hypothetical-protein	unknown	unknown	gnl|CDD|379252
CP020115.1|AVZ31727.1|54267_56463_-|ABC-transporter-ATP-binding/permease-protein	unknown	unknown	gnl|CDD|224054
CP020115.1|AVZ31733.1|73079_73376_-|hypothetical-protein	unknown	unknown	unknown
CP020115.1|AVZ31719.1|26563_28411_-|serine/threonine-protein-kinase-B	unknown	unknown	gnl|CDD|270916
CP020115.1|AVZ31723.1|44231_44627_-|hypothetical-protein	unknown	unknown	gnl|CDD|369598
CP020115.1|AVZ31716.1|17576_18140_-|3-oxoacyl-[acyl-carrier-protein]-reductase-FabG	unknown	unknown	gnl|CDD|212491
CP020115.1|AVZ31726.1|53423_54116_-|hypothetical-protein	unknown	unknown	gnl|CDD|378452
CP020115.1|AVZ31731.1|65350_66379_-|hypothetical-protein	unknown	unknown	gnl|CDD|379252
CP020115.1|AVZ31734.1|73482_74424_-|hypothetical-protein	unknown	unknown	gnl|CDD|212669
CP020115.1|AVZ31722.1|42294_43500_-|group-II-intron-encoded-protein-LtrA	RT	pfam00078_RT_CAS-I:CAS-III	gnl|CDD|275209

HMMScan for Signature genes

Protein	Cas_name	Cas_description	E-value	Identity	Coverage
CP020115.1|AVZ31717.1|18148_18523_-|putative-transposase	Cas14u_CAS-V	-	2.5e-19	64.5	0.2
CP020115.1|AVZ31722.1|42294_43500_-|group-II-intron-encoded-protein-LtrA	RT	pfam00078_RT_CAS-I:CAS-III	4.8e-33	109.3	0.1

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
CP020115.1|AVZ31727.1|54267_56463_-|ABC-transporter-ATP-binding/permease-protein	gnl|CDD|224054	COG1131, CcmA, ABC-type multidrug transport system, ATPase component [Defense mechanisms].	2.12337e-62
CP020115.1|AVZ31721.1|39111_39957_-|tyrosine-recombinase-XerD	gnl|CDD|234698	PRK00236, xerC, site-specific tyrosine recombinase XerC; Reviewed.	6.7904e-56
CP020115.1|AVZ31725.1|49907_51290_-|hypothetical-protein	gnl|CDD|376573	pfam01609, DDE_Tnp_1, Transposase DDE domain. Transposase proteins are necessary for efficient DNA transposition. This domain is a member of the DDE superfamily, which contain three carboxylate residues that are believed to be responsible for coordinating metal ions needed for catalysis. The catalytic activity of this enzyme involves DNA cleavage at a specific site followed by a strand transfer reaction. This family contains transposases for IS4, IS421, IS5377, IS427, IS402, IS1355, IS5, which was original isolated in bacteriophage lambda.	2.57981e-11
CP020115.1|AVZ31728.1|56753_57992_-|hypothetical-protein	gnl|CDD|212669	cd10227, ParM_like, Plasmid segregation protein ParM and similar proteins. ParM is a plasmid-encoded bacterial homolog of actin, which polymerizes into filaments similar to F-actin, and plays a vital role in plasmid segregation. ParM filaments segregate plasmids paired at midcell into the individual daughter cells. This subfamily also contains Thermoplasma acidophilum Ta0583, an active ATPase at physiological temperatures, which has a propensity to form filaments.	1.93626e-20
CP020115.1|AVZ31717.1|18148_18523_-|putative-transposase	gnl|CDD|284650	pfam07282, OrfB_Zn_ribbon, Putative transposase DNA-binding domain. This putative domain is found at the C-terminus of a large number of transposase proteins. This domain contains four conserved cysteines suggestive of a zinc binding domain. Given the need for transposases to bind DNA as well as the large number of DNA-binding zinc fingers we hypothesize this domain is DNA-binding.	8.7676e-16
CP020115.1|AVZ31715.1|15395_16424_-|hypothetical-protein	gnl|CDD|379252	pfam13546, DDE_5, DDE superfamily endonuclease. This family of proteins are related to pfam00665 and are probably endonucleases of the DDE superfamily. Transposase proteins are necessary for efficient DNA transposition. This domain is a member of the DDE superfamily, which contain three carboxylate residues that are believed to be responsible for coordinating metal ions needed for catalysis. The catalytic activity of this enzyme involves DNA cleavage at a specific site followed by a strand transfer reaction.	0.000432813
CP020115.1|AVZ31718.1|25511_26531_-|hypothetical-protein	gnl|CDD|274350	TIGR02917, TPR_domain_protein, putative PEP-CTERM system TPR-repeat lipoprotein. This protein family occurs in strictly within a subset of Gram-negative bacterial species with the proposed PEP-CTERM/exosortase system, analogous to the LPXTG/sortase system common in Gram-positive bacteria. This protein occurs in a species if and only if a transmembrane histidine kinase (TIGR02916) and a DNA-binding response regulator (TIGR02915) also occur. The present of tetratricopeptide repeats (TPR) suggests protein-protein interaction, possibly for the regulation of PEP-CTERM protein expression, since many PEP-CTERM proteins in these genomes are preceded by a proposed DNA binding site for the response regulator.	0.000728892
CP020115.1|AVZ31719.1|26563_28411_-|serine/threonine-protein-kinase-B	gnl|CDD|270916	cd14014, STKc_PknB_like, Catalytic domain of bacterial Serine/Threonine kinases, PknB and similar proteins. STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. This subfamily includes many bacterial eukaryotic-type STKs including Staphylococcus aureus PknB (also called PrkC or Stk1), Bacillus subtilis PrkC, and Mycobacterium tuberculosis Pkn proteins (PknB, PknD, PknE, PknF, PknL, and PknH), among others. S. aureus PknB is the only eukaryotic-type STK present in this species, although many microorganisms encode for several such proteins. It is important for the survival and pathogenesis of S. aureus as it is involved in the regulation of purine and pyrimidine biosynthesis, cell wall metabolism, autolysis, virulence, and antibiotic resistance. M. tuberculosis PknB is essential for growth and it acts on diverse substrates including proteins involved in peptidoglycan synthesis, cell division, transcription, stress responses, and metabolic regulation. B. subtilis PrkC is located at the inner membrane of endospores and functions to trigger spore germination. Bacterial STKs in this subfamily show varied domain architectures. The well-characterized members such as S. aureus and M. tuberculosis PknB, and B. subtilis PrkC, contain an N-terminal cytosolic kinase domain, a transmembrane (TM) segment, and mutliple C-terminal extracellular PASTA domains. The PknB subfamily is part of a larger superfamily that includes the catalytic domains of other protein STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase.	2.80642e-42
CP020115.1|AVZ31723.1|44231_44627_-|hypothetical-protein	gnl|CDD|369598	pfam07924, NuiA, Nuclease A inhibitor-like protein. This family consists of protein sequences that are similar to the nuclease A inhibitor expressed by bacteria of the genus Anabaena ((NuiA). This sequence is organized to form an alpha-beta-alpha sandwich fold, which is similar to the PR-1-like fold. NuiA interacts with nuclease A by means of residues located at one end of the molecule, including residues making up the loop between helices III and IV and the loop between strands C and D. The mechanism of inhibition of nuclease A by NuiA is as yet incompletely understood.	2.28653e-62
CP020115.1|AVZ31716.1|17576_18140_-|3-oxoacyl-[acyl-carrier-protein]-reductase-FabG	gnl|CDD|212491	cd05233, SDR_c, classical (c) SDRs. SDRs are a functionally diverse family of oxidoreductases that have a single domain with a structurally conserved Rossmann fold (alpha/beta folding pattern with a central beta-sheet), an NAD(P)(H)-binding region, and a structurally diverse C-terminal region. Classical SDRs are typically about 250 residues long, while extended SDRs are approximately 350 residues. Sequence identity between different SDR enzymes are typically in the 15-30% range, but the enzymes share the Rossmann fold NAD-binding motif and characteristic NAD-binding and catalytic sequence patterns. These enzymes catalyze a wide range of activities including the metabolism of steroids, cofactors, carbohydrates, lipids, aromatic compounds, and amino acids, and act in redox sensing. Classical SDRs have an TGXXX[AG]XG cofactor binding motif and a YXXXK active site motif, with the Tyr residue of the active site motif serving as a critical catalytic residue (Tyr-151, human prostaglandin dehydrogenase (PGDH) numbering). In addition to the Tyr and Lys, there is often an upstream Ser (Ser-138, PGDH numbering) and/or an Asn (Asn-107, PGDH numbering) contributing to the active site; while substrate binding is in the C-terminal region, which determines specificity. The standard reaction mechanism is a 4-pro-S hydride transfer and proton relay involving the conserved Tyr and Lys, a water molecule stabilized by Asn, and nicotinamide. Extended SDRs have additional elements in the C-terminal region, and typically have a TGXXGXXG cofactor binding motif. Complex (multidomain) SDRs such as ketoreductase domains of fatty acid synthase have a GGXGXXG NAD(P)-binding motif and an altered active site motif (YXXXN). Fungal type ketoacyl reductases have a TGXXXGX(1-2)G NAD(P)-binding motif. Some atypical SDRs have lost catalytic activity and/or have an unusual NAD(P)-binding motif and missing or unusual active site residues. Reactions catalyzed within the SDR family include isomerization, decarboxylation, epimerization, C=N bond reduction, dehydratase activity, dehalogenation, Enoyl-CoA reduction, and carbonyl-alcohol oxidoreduction.	1.97423e-40
CP020115.1|AVZ31722.1|42294_43500_-|group-II-intron-encoded-protein-LtrA	gnl|CDD|275209	TIGR04416, hypothetical_protein, group II intron reverse transcriptase/maturase. Members of this protein family are multifunctional proteins encoded in most examples of bacterial group II introns. These group II introns are mobile selfish genetic elements, often with multiple highly identical copies per genome. Member proteins have an N-terminal reverse transcriptase (RNA-directed DNA polymerase) domain (pfam00078) followed by an RNA-binding maturase domain (pfam08388). Some members of this family may have an additional C-terminal DNA endonuclease domain that this model does not cover. A region of the group II intron ribozyme structure should be detectable nearby on the genome by Rfam model RF00029. [Mobile and extrachromosomal element functions, Other].	9.78754e-80
CP020115.1|AVZ31731.1|65350_66379_-|hypothetical-protein	gnl|CDD|379252	pfam13546, DDE_5, DDE superfamily endonuclease. This family of proteins are related to pfam00665 and are probably endonucleases of the DDE superfamily. Transposase proteins are necessary for efficient DNA transposition. This domain is a member of the DDE superfamily, which contain three carboxylate residues that are believed to be responsible for coordinating metal ions needed for catalysis. The catalytic activity of this enzyme involves DNA cleavage at a specific site followed by a strand transfer reaction.	0.000432813
CP020115.1|AVZ31734.1|73482_74424_-|hypothetical-protein	gnl|CDD|212669	cd10227, ParM_like, Plasmid segregation protein ParM and similar proteins. ParM is a plasmid-encoded bacterial homolog of actin, which polymerizes into filaments similar to F-actin, and plays a vital role in plasmid segregation. ParM filaments segregate plasmids paired at midcell into the individual daughter cells. This subfamily also contains Thermoplasma acidophilum Ta0583, an active ATPase at physiological temperatures, which has a propensity to form filaments.	7.47889e-36
CP020115.1|AVZ31726.1|53423_54116_-|hypothetical-protein	gnl|CDD|378452	pfam10592, AIPR, AIPR protein. This family of proteins was identified in as an abortive infection phage resistance protein often found in restriction modification system operons.	1.41319e-29

Protein sequences

>CP020115.1|AVZ31724.1|45486_45705_-|hypothetical-protein
MELWQPEGASYYNGELIVQPQQRWTPEEQLEMLDTHPLFTGFCPQCGAEFDRDYVSRVHWDCNNCGWKDDSI
>CP020115.1|AVZ31723.1|44231_44627_-|hypothetical-protein
MTKTNAEILEQLKQASEGLLFMSESEYPCDICLWSDATPEQVVHLTNHPQDTPVEVEGVDDFFSVATTAQDWHSEEEKETVHKFQTLVQILKANLSNLQVYRLGEVEVDVYVVGQTPTGDLAGIATKIVET
>CP020115.1|AVZ31722.1|42294_43500_-|group-II-intron-encoded-protein-LtrA
MDADISKCFDKINHQALLKKINTFPTLNRLIKKWLKAGVFDNNEWLPTKQGTPQGGVISPLLANIALHGMEEEINKVADSLPGNKKKNRYALSFIRYADDFIIIHPSFDVVNKCKDRISEWLSEMGLELKPSKTKIVHTLKPEPNIGTQSSINTGGFDFLGFNVRQYQVGKTHSGKNSKGNLLGFKTLIKPSNKAIRKHYDEICKIIDTYKSAPQEALIKRLNPIIRGWTNYYSSVVSKEIFSSLDHIIFHKLQTWAKRRHPNKSKSWISSKYWGTFGNNNWVFSTKYEGKITFTLLKYVKTEIVRHIKVEGKKSPFDGNLVYWSERRGKSTLLPLRVTTLLKSQKGKCNHCGLHFREDDVMEVDHIIPKSKGGKNDYKNLQLLHRHCHDVKTAISGSRYA
>CP020115.1|AVZ31721.1|39111_39957_-|tyrosine-recombinase-XerD
MLNHSSTLTNTELLDLWLHGKSKNTIDGYRRHAERFFCHVNKPLADCTLMDIQLWVMTLHCSDNSKRVAVGAIKSLLSFAKQLGVVSANLGILIKSPKSRNRLAERILTQEEVKLLISSTTNSRDHAIIRLFYLAGLRVSELCGLKWRDLKARGDGGQITVFGKGEKTRTVLVGAGMWKEINSLKGYAKHDDPVFISAKGGHLCRSMVFHIVKKAANRAGIEGNVSPHWLRHSHASHSLDRGAPIHLLQKTLGHSSVAITEMYLHARPTDSSGLYLPDDDE
>CP020115.1|AVZ31720.1|37602_37914_-|hypothetical-protein
MVKIHAPIHIQIYEREGTQWFFHGGNIFHNPHGISTDLESTLERNGLTKIKVLVELFRVNGGKAGLYLADIRDKKYYYCGQKWEDVKGLLRELGIGRDEPSSS
>CP020115.1|AVZ31719.1|26563_28411_-|serine/threonine-protein-kinase-B
MSYCINPLCDHRQNPDDAQNCLSCGTSLVINNRIRLIKPLRALNDNPFSYTEVFEVDDAGTQWNPVRKQRVMKVLKLNSPKLVELIERESLSLRLIHHPNIPKSTLDDFFTFVPNKSSLILHCLVMDKIKGQNLEKWIESNGRILQSQALEWLKQLVEILAAVHAANFFHRDIKPSNIILQSSGQLALVDFGVARRVTSTYLAKISGSGGDSTSRGGKYEITSVGTPRYSPPEQTDGQAVPQSDFYALGRTFVHLLTAIQLIDLPTDKQTGRLIWRNKAPQIDKPFADFIDELMAPFPGQRPQTTRVILQRLEKLPLQSKFHSFTKSKTFIISAIITSIALGSFGIFKIALPGLANALVSEGQNLEAENKSENAQQLFKWAIKIHPQSSNNISSFYFGKAGRNLTNLEVAKKYLEKAIKYNPQDANAYINLALVCQNLRQLECAIANQKKAISLHPSNGILYYDLGSIYDDFGKYKLAEEKYNIAIKLSQNPAAPINNLSRLKNIEEKYHEAEALALKGLKLTENPEIKTTLYKNLGWAKLGQRKYSEANNYLQKSRDIDATKTDTFCLLAQAQDALGEKTKARIYWEVCIIAESNLPEVFNWRQQLLDRLIEKL
>CP020115.1|AVZ31718.1|25511_26531_-|hypothetical-protein
MVNFMMFIPKVINNTFMALLLVISNSSSVWSQPPRLAKGVCHDPVGRVINGGDVHLSAGSLICPEDRIQPAKGSMVEVICLTSRRILEIKQSDSAAQVCAERVSQPRRLCILDANCRNTRGSEERENPTLIAPYVGRIINPRPKLSWLEVLGATSYIVQVEGSGGNWSKEIPKTSLEYPLSEPAMEWGRIYKINVIANKGDEPVSASSSVTIVLPQEEAQEINRVVEKIQNLNLSADELAIDLDAVYMSKNLLTEAIGVLEARVRNGTQNPAIYRMLGDRYLETGLLETHSLAAAIRAYAEAVKLARQKNDAEELAKAQAGVKLLESQSQAPTRTNAAQ
>CP020115.1|AVZ31717.1|18148_18523_-|putative-transposase
MVFVEDLNLVGLSCGMLGKHCLDAGFGQFFHILEQTCMKRDVYFQKVDSRKTSQICPNCGVETGKKELSERTHVCSNCGYTTDRDVAAAQVVLVRGLAAVGHTVKMLAEGKFIGIPVKQESSHL
>CP020115.1|AVZ31716.1|17576_18140_-|3-oxoacyl-[acyl-carrier-protein]-reductase-FabG
MSSQKFTAPNDFTVVDVKKEPQVEKWAKDVLSKYDPPDIVINNAAITNYPAPLWQVPSDEFSELIDINIKGVVNIIRHFVPAMLKNKRGIIVNFSSGWGRSTSPEVAPYCASKWAIEGLTRSLAQELPAGMAAVPLNPGIIHTDMLSISFGEEAVNYTPVSDWVLKAVPYILNLKPTDNGMPLTVSM
>CP020115.1|AVZ31715.1|15395_16424_-|hypothetical-protein
MLVISMRFTKLNYCQYLLSSQINYTITNLAEHLEHISHDAINYYLKREKLTPRLLWENVKDVIELDANSYIIFDDSILDKRYSKEIEMVRKQYSGNEHGIIKGIGVVSCVYVNPKVQRFWVIDYRIFNPDVDGKTKIDHVKDMLQNLVYQKLLPFDTVLMDTWYAVHSLMLYIDSLEKVYYCPLKNNRLVDDTLGKEKYKRIDLLQWSEEELECGKIIKIKGFPAQKKVKLFRVAVSTNRTDYVATNDLSQSSTNVVQKVCKIRWKIEEFHREIKQLTGIESCQCRKARLQRNHIACAMLVWIRLKNLAYQTGQTVYQIKHNLLSNYLIQQLKRPSISMCLV
>CP020115.1|AVZ31725.1|49907_51290_-|hypothetical-protein
MNTPIISKSVKILKQKFTHSLGLPIHQLLPSEVIQEELAQLNIKYRQRLFDPICTLWAFLSQVLDVDKSCHNAVSRVIAYLVGSGVELPSTDTSAYCQARQRLPEELIQKLFGKVAVGLESQVNETHLWCGRKVKVIDGSTVSMPDTPDNQNEYPQPSSQKIGCGFPLAKIGALFSIATGAAVALAIDVFNTHDIQLARRLYEFLSPGDVLLGDRAFCAYADIFQIKSLGGDAVFRKHQGRQTQMRRGKIVGSCDKIVTWSKPKKCPQGLSQEEFNSLPNTLIVREIYFYIFIPGFRTEQVSLITTLLDTKAYSTLDITSLYEKRWDVEVDLKHLKTTLGMDILRSKTPAMVRKEIWAFLLAYNLLRTLMWSAGNSYGVSPLRLSLQGTRQHFDNFIPQFIAATNSDRHQIYSTLLKVIVHKEVPLRQARSEPRVRKRRPKSYPLMQELRQRYHDNLKTA
>CP020115.1|AVZ31726.1|53423_54116_-|hypothetical-protein
MPKTWILKIDNYFNANPNCIIATAHIDKFPTDLPLEPNIREPNRRSATYRQILDSLTIQPEKFFERHSGIVLCANKVKPNKTQTELELEILEASEGGSDGIINGGHTVLAFEQARNYKYDLSQAKVKVTIHIGLNEAEAKDIALASNTTSPVDSRSKVNARGDYEFIKQYLAELERTEDRKFRVAYYQNQTGAPRNPQCNGEQRDFVKLGQKVAIANHLFSVQSNFIYNI
>CP020115.1|AVZ31727.1|54267_56463_-|ABC-transporter-ATP-binding/permease-protein
MQTLQLTAHTNPIQQCTVTQLPFTIGSDPNNDLVVSDKDVDSYHAAILLGKNQYWLFDVGSSSGTRIGSTQLRHQQSQALSDGNLIQVGSIIIEIKITNPPTAKAHIFTPVAKTQLFNKKPENPILQNLRLDAINLYKSTSLFFGTTLINKISVSFLPQEFIVIAGVSGGGKSTLMDALNGQRRANSGKVMVNGVNLYRHFSAFKHSIGYVPQDDIVHPELTVREALHYAAQLRLPHATPEIRQQRIQAVLEQLHLTPRKHVPIKQLSGGQRKRVSIGVELITKPSLFFLDEATSGLDPGTELQIMELLRDLAHNGSTIVLITHATKNVAITDFVVFLAKGGRLAYFGPPKMAFDYFKVPDFDAIYNKVERQLSPEKWEERFKESVFHRKFISDRQESIPQTKVTPVAPRINYWQQWWVLCRRNLTILFKNKMSLSLMLTGAPILGGFNFLLWKSDLFDTKTGNPGQSITMLFVTVIMAVMAGALATMPEIAKETAIYRRERSAGLGVLPYLASKFHLSFLLALYQSAVLLGCMVSAVTVPESALPGMYVTLFLASFGSMVLGLLVSAIAPNQSVAPLLTILVLVPQVIFGGGILPVSEFKDSGKFLNQLMLTKYPFESLVTLSGLGEDVAKDPCWQKPERERRRLSVQESNACDCYGNNVFKQCQFPGLGTVDRSSFLSSDVFKARQLISHVHQTYGDIFSINVMLSLMQMTFLIGAIAILLFVFQSAKG
>CP020115.1|AVZ31728.1|56753_57992_-|hypothetical-protein
MTKNQDKSKNVTRLVLTCDLGGSQTKAIAQIYPDGIPIVLAMSPEVADVSQGSIEWLIQQNQFFHGNNAWVGLGEEYYVLGELAKTMFAGTAAIRDLKYQYALPKIMAMLWLACRNFNLNSFSEVFVQLLLPPGEVSDGEQLAIELAQALKKGVTTPTVRLKGKLRNFDVSREGSGIMAYYRRSIQNKYFQNNIGMLMLGYRNASFYLSQKGNPSLAESTQWGMNWVVAQFVERTAVGLSKYDSRLIPALLAAGKGDFDDLRALSRKTTESGIESDLKLFQKLLPDVRDEYCLAVLRWMRNIADLDEIVICGGTAEFVRQELTEHFENEGIPIIWNGNVSIPAKLDTLDLGDRVTDVWTTHISHIKMLDKNLGHDRQGRTLVPTDNQPTYYNGRVPEEVWIKQGFMPMHEGV
>CP020115.1|AVZ31729.1|58007_58517_-|hypothetical-protein
MTDAKHQRISIKRLDDSVDGQVISYLQSDPFDENESFPELVLSTLKAHWLPLTLLSKGVGGEKLRQAGIRAIAKLEAQISTIRRICGLDIEPVHVAPVVINQTFVSANDKTSTWGEISDSHSNNSSQSNAQTNEDEIDEDDDDWDVEAALMRMPKPEELRDANRMMGLG
>CP020115.1|AVZ31730.1|61505_62069_-|hypothetical-protein
MANTERESINFKLPKTLTNALRTAARERKTTATDLVIQGLHHILGQVPGTETSVETRLHQIEAFLTQMANGQDAGVEDSSKQRLSLLEQKTEAISLRLAQIEGAISLLGQRSATPQRRQSYQYHPPQLELQAYQGVNLAKRLGVDLATLERERNNQSSKDFERWCRSKDPGSVGWRFGDDGLFHPIK
>CP020115.1|AVZ31731.1|65350_66379_-|hypothetical-protein
MLVISMRFTKLNYCQYLLSSQINYTITNLAEHLEHISHDAINYYLKREKLTPRLLWENVKDVIELDANSYIIFDDSILDKRYSKEIEMVRKQYSGNEHGIIKGIGVVSCVYVNPKVQRFWVIDYRIFNPDVDGKTKIDHVKDMLQNLVYQKLLPFDTVLMDTWYAVHSLMLYIDSLEKVYYCPLKNNRLVDDTLGKEKYKRIDLLQWSEEELECGKIIKIKGFPAQKKVKLFRVAVSTNRTDYVATNDLSQSSTNVVQKVCKIRWKIEEFHREIKQLTGIESCQCRKARLQRNHIACAMLVWIRLKNLAYQTGQTVYQIKHNLLSNYLIQQLKRPSISMCLV
>CP020115.1|AVZ31732.1|67381_68173_-|hypothetical-protein
MKNSAIETINSINRVRGVSNNMMAYRLSLIMQQAKENSTPLATENQALPQERQEELTKLLSKSPTTQNLENFRQKLKDRYYSEYLSKFEAKLSVESQKQDTPTRVERLIAEKKLQFLQMQQSKLEEKPDHNHSKASPEINSSLPEQSEATNKLLIPAMMGLVVAKGQNTDERTRIYEGVRYKLQLLMKEGMQFLSINRKDKKPEKAFSAYKNHKDSQFKVLENNLTDEETQGIIAFNQQRIVQEAQNQGKHNQDKDKNAELGD
>CP020115.1|AVZ31733.1|73079_73376_-|hypothetical-protein
MTPEKVRIKDSYRERIFAESQQLDKSYLETLYFIIDCYFAFKKGVFPTQQVVTQVTHTPAFNPIATNPSPQDQPSATPSPPENPKDSDGETFTLDFDL
>CP020115.1|AVZ31734.1|73482_74424_-|hypothetical-protein
MPNIHALQKIFPAGFDNGYGSLKLFVDGFEVVRIPSYISTAEMEDVPGRIILDGAAYTVGESAFRTGYHFDRNTDHNENKIKNALLMLLGGLAHLPHRKAWHLKLVVSLHDIGLAEDITQELNGEYQPILAGKKSDVTIEIIKVVPEGMGALFGHPLPPKLTVLDFGNGTTLYSRYNQGKREVHTPYPAGVEVLIEDIAEKMKRLNGGKIGNASQIRFCLEGGHTRYSRDIDIKEIYSNCLKDWYEKFLKKPVGLTLDAKHQGDEIWAIGGGCLLPGLKKLLEKNGFKILDNPVEANACGLLEMAKVMMNKNL

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity
CP020115_1	1.1|36349|35|CP020115|CRT	36349-36383	35	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36349-36383	0	1.0
CP020115_1	1.1|36349|35|CP020115|CRT	36349-36383	35	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36718-36752	0	1.0
CP020115_1	1.2|36412|32|CP020115|CRT	36412-36443	32	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36412-36443	0	1.0
CP020115_1	1.2|36412|32|CP020115|CRT	36412-36443	32	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36535-36566	0	1.0
CP020115_1	1.2|36412|32|CP020115|CRT	36412-36443	32	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36658-36689	0	1.0
CP020115_1	1.2|36412|32|CP020115|CRT	36412-36443	32	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36781-36812	0	1.0
CP020115_1	1.3|36472|35|CP020115|CRT	36472-36506	35	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36472-36506	0	1.0
CP020115_1	1.3|36472|35|CP020115|CRT	36472-36506	35	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36841-36875	0	1.0
CP020115_1	1.4|36535|32|CP020115|CRT	36535-36566	32	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36412-36443	0	1.0
CP020115_1	1.4|36535|32|CP020115|CRT	36535-36566	32	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36535-36566	0	1.0
CP020115_1	1.4|36535|32|CP020115|CRT	36535-36566	32	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36658-36689	0	1.0
CP020115_1	1.4|36535|32|CP020115|CRT	36535-36566	32	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36781-36812	0	1.0
CP020115_1	1.5|36595|35|CP020115|CRT	36595-36629	35	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36595-36629	0	1.0
CP020115_1	1.6|36658|32|CP020115|CRT	36658-36689	32	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36412-36443	0	1.0
CP020115_1	1.6|36658|32|CP020115|CRT	36658-36689	32	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36535-36566	0	1.0
CP020115_1	1.6|36658|32|CP020115|CRT	36658-36689	32	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36658-36689	0	1.0
CP020115_1	1.6|36658|32|CP020115|CRT	36658-36689	32	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36781-36812	0	1.0
CP020115_1	1.7|36718|35|CP020115|CRT	36718-36752	35	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36349-36383	0	1.0
CP020115_1	1.7|36718|35|CP020115|CRT	36718-36752	35	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36718-36752	0	1.0
CP020115_1	1.8|36781|32|CP020115|CRT	36781-36812	32	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36412-36443	0	1.0
CP020115_1	1.8|36781|32|CP020115|CRT	36781-36812	32	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36535-36566	0	1.0
CP020115_1	1.8|36781|32|CP020115|CRT	36781-36812	32	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36658-36689	0	1.0
CP020115_1	1.8|36781|32|CP020115|CRT	36781-36812	32	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36781-36812	0	1.0
CP020115_1	1.9|36841|35|CP020115|CRT	36841-36875	35	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36472-36506	0	1.0
CP020115_1	1.9|36841|35|CP020115|CRT	36841-36875	35	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36841-36875	0	1.0
CP020115_2	2.1|45868|50|CP020115|CRISPRCasFinder	45868-45917	50	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	45868-45917	0	1.0
CP020115_2	2.2|45941|24|CP020115|CRISPRCasFinder	45941-45964	24	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	45941-45964	0	1.0
CP020115_2	2.3|45988|25|CP020115|CRISPRCasFinder	45988-46012	25	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	45988-46012	0	1.0
CP020115_2	2.4|46036|37|CP020115|CRISPRCasFinder	46036-46072	37	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	46036-46072	0	1.0
CP020115_1	1.1|36349|35|CP020115|CRT	36349-36383	35	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36595-36629	1	0.971
CP020115_1	1.3|36472|35|CP020115|CRT	36472-36506	35	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36595-36629	1	0.971
CP020115_1	1.5|36595|35|CP020115|CRT	36595-36629	35	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36349-36383	1	0.971
CP020115_1	1.5|36595|35|CP020115|CRT	36595-36629	35	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36472-36506	1	0.971
CP020115_1	1.5|36595|35|CP020115|CRT	36595-36629	35	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36718-36752	1	0.971
CP020115_1	1.5|36595|35|CP020115|CRT	36595-36629	35	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36841-36875	1	0.971
CP020115_1	1.7|36718|35|CP020115|CRT	36718-36752	35	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36595-36629	1	0.971
CP020115_1	1.9|36841|35|CP020115|CRT	36841-36875	35	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36595-36629	1	0.971
CP020115_1	1.1|36349|35|CP020115|CRT	36349-36383	35	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36472-36506	2	0.943
CP020115_1	1.1|36349|35|CP020115|CRT	36349-36383	35	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36841-36875	2	0.943
CP020115_1	1.3|36472|35|CP020115|CRT	36472-36506	35	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36349-36383	2	0.943
CP020115_1	1.3|36472|35|CP020115|CRT	36472-36506	35	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36718-36752	2	0.943
CP020115_1	1.7|36718|35|CP020115|CRT	36718-36752	35	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36472-36506	2	0.943
CP020115_1	1.7|36718|35|CP020115|CRT	36718-36752	35	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36841-36875	2	0.943
CP020115_1	1.9|36841|35|CP020115|CRT	36841-36875	35	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36349-36383	2	0.943
CP020115_1	1.9|36841|35|CP020115|CRT	36841-36875	35	NZ_CP020115	Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence	36718-36752	2	0.943
CP020115_2	2.2|45941|24|CP020115|CRISPRCasFinder	45941-45964	24	NZ_CP029452	Sinorhizobium fredii CCBAU 25509 plasmid pSF25509b, complete sequence	1213582-1213605	3	0.875
CP020115_2	2.2|45941|24|CP020115|CRISPRCasFinder	45941-45964	24	NZ_CP023071	Sinorhizobium fredii CCBAU 83666 plasmid pSF83666b, complete sequence	1223061-1223084	3	0.875
CP020115_2	2.2|45941|24|CP020115|CRISPRCasFinder	45941-45964	24	NZ_CP029232	Sinorhizobium fredii CCBAU 45436 plasmid pSF45436b, complete sequence	864288-864311	3	0.875

1. spacer 1.1|36349|35|CP020115|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 0, identity: 1.0

attgtcgggagggggatgatctcaccattaacccg	CRISPR spacer
attgtcgggagggggatgatctcaccattaacccg	Protospacer
***********************************

2. spacer 1.1|36349|35|CP020115|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 0, identity: 1.0

attgtcgggagggggatgatctcaccattaacccg	CRISPR spacer
attgtcgggagggggatgatctcaccattaacccg	Protospacer
***********************************

3. spacer 1.2|36412|32|CP020115|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 0, identity: 1.0

gttttcaagaaggggagcgcgcgaaagatata	CRISPR spacer
gttttcaagaaggggagcgcgcgaaagatata	Protospacer
********************************

4. spacer 1.2|36412|32|CP020115|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 0, identity: 1.0

gttttcaagaaggggagcgcgcgaaagatata	CRISPR spacer
gttttcaagaaggggagcgcgcgaaagatata	Protospacer
********************************

5. spacer 1.2|36412|32|CP020115|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 0, identity: 1.0

gttttcaagaaggggagcgcgcgaaagatata	CRISPR spacer
gttttcaagaaggggagcgcgcgaaagatata	Protospacer
********************************

6. spacer 1.2|36412|32|CP020115|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 0, identity: 1.0

gttttcaagaaggggagcgcgcgaaagatata	CRISPR spacer
gttttcaagaaggggagcgcgcgaaagatata	Protospacer
********************************

7. spacer 1.3|36472|35|CP020115|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 0, identity: 1.0

attgtcaggagggggatgatctcaccattaactcg	CRISPR spacer
attgtcaggagggggatgatctcaccattaactcg	Protospacer
***********************************

8. spacer 1.3|36472|35|CP020115|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 0, identity: 1.0

attgtcaggagggggatgatctcaccattaactcg	CRISPR spacer
attgtcaggagggggatgatctcaccattaactcg	Protospacer
***********************************

9. spacer 1.4|36535|32|CP020115|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 0, identity: 1.0

gttttcaagaaggggagcgcgcgaaagatata	CRISPR spacer
gttttcaagaaggggagcgcgcgaaagatata	Protospacer
********************************

10. spacer 1.4|36535|32|CP020115|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 0, identity: 1.0

gttttcaagaaggggagcgcgcgaaagatata	CRISPR spacer
gttttcaagaaggggagcgcgcgaaagatata	Protospacer
********************************

11. spacer 1.4|36535|32|CP020115|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 0, identity: 1.0

gttttcaagaaggggagcgcgcgaaagatata	CRISPR spacer
gttttcaagaaggggagcgcgcgaaagatata	Protospacer
********************************

12. spacer 1.4|36535|32|CP020115|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 0, identity: 1.0

gttttcaagaaggggagcgcgcgaaagatata	CRISPR spacer
gttttcaagaaggggagcgcgcgaaagatata	Protospacer
********************************

13. spacer 1.5|36595|35|CP020115|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 0, identity: 1.0

attgtcgggagggggatgatctcaccattaactcg	CRISPR spacer
attgtcgggagggggatgatctcaccattaactcg	Protospacer
***********************************

14. spacer 1.6|36658|32|CP020115|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 0, identity: 1.0

gttttcaagaaggggagcgcgcgaaagatata	CRISPR spacer
gttttcaagaaggggagcgcgcgaaagatata	Protospacer
********************************

15. spacer 1.6|36658|32|CP020115|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 0, identity: 1.0

gttttcaagaaggggagcgcgcgaaagatata	CRISPR spacer
gttttcaagaaggggagcgcgcgaaagatata	Protospacer
********************************

16. spacer 1.6|36658|32|CP020115|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 0, identity: 1.0

gttttcaagaaggggagcgcgcgaaagatata	CRISPR spacer
gttttcaagaaggggagcgcgcgaaagatata	Protospacer
********************************

17. spacer 1.6|36658|32|CP020115|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 0, identity: 1.0

gttttcaagaaggggagcgcgcgaaagatata	CRISPR spacer
gttttcaagaaggggagcgcgcgaaagatata	Protospacer
********************************

18. spacer 1.7|36718|35|CP020115|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 0, identity: 1.0

attgtcgggagggggatgatctcaccattaacccg	CRISPR spacer
attgtcgggagggggatgatctcaccattaacccg	Protospacer
***********************************

19. spacer 1.7|36718|35|CP020115|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 0, identity: 1.0

attgtcgggagggggatgatctcaccattaacccg	CRISPR spacer
attgtcgggagggggatgatctcaccattaacccg	Protospacer
***********************************

20. spacer 1.8|36781|32|CP020115|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 0, identity: 1.0

gttttcaagaaggggagcgcgcgaaagatata	CRISPR spacer
gttttcaagaaggggagcgcgcgaaagatata	Protospacer
********************************

21. spacer 1.8|36781|32|CP020115|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 0, identity: 1.0

gttttcaagaaggggagcgcgcgaaagatata	CRISPR spacer
gttttcaagaaggggagcgcgcgaaagatata	Protospacer
********************************

22. spacer 1.8|36781|32|CP020115|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 0, identity: 1.0

gttttcaagaaggggagcgcgcgaaagatata	CRISPR spacer
gttttcaagaaggggagcgcgcgaaagatata	Protospacer
********************************

23. spacer 1.8|36781|32|CP020115|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 0, identity: 1.0

gttttcaagaaggggagcgcgcgaaagatata	CRISPR spacer
gttttcaagaaggggagcgcgcgaaagatata	Protospacer
********************************

24. spacer 1.9|36841|35|CP020115|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 0, identity: 1.0

attgtcaggagggggatgatctcaccattaactcg	CRISPR spacer
attgtcaggagggggatgatctcaccattaactcg	Protospacer
***********************************

25. spacer 1.9|36841|35|CP020115|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 0, identity: 1.0

attgtcaggagggggatgatctcaccattaactcg	CRISPR spacer
attgtcaggagggggatgatctcaccattaactcg	Protospacer
***********************************

26. spacer 2.1|45868|50|CP020115|CRISPRCasFinder matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 0, identity: 1.0

cttgagtgcgatgggcaaagccccgccgtaggcgatcgccactgataact	CRISPR spacer
cttgagtgcgatgggcaaagccccgccgtaggcgatcgccactgataact	Protospacer
**************************************************

27. spacer 2.2|45941|24|CP020115|CRISPRCasFinder matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 0, identity: 1.0

gctactcctgcggtgcgctcatca	CRISPR spacer
gctactcctgcggtgcgctcatca	Protospacer
************************

28. spacer 2.3|45988|25|CP020115|CRISPRCasFinder matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 0, identity: 1.0

tgcgagttctgcggtgcgctcatca	CRISPR spacer
tgcgagttctgcggtgcgctcatca	Protospacer
*************************

29. spacer 2.4|46036|37|CP020115|CRISPRCasFinder matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 0, identity: 1.0

agtgtgagtgtttgtacctatgtcccatgtcccaact	CRISPR spacer
agtgtgagtgtttgtacctatgtcccatgtcccaact	Protospacer
*************************************

30. spacer 1.1|36349|35|CP020115|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 1, identity: 0.971

attgtcgggagggggatgatctcaccattaacccg	CRISPR spacer
attgtcgggagggggatgatctcaccattaactcg	Protospacer
********************************.**

31. spacer 1.3|36472|35|CP020115|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 1, identity: 0.971

attgtcaggagggggatgatctcaccattaactcg	CRISPR spacer
attgtcgggagggggatgatctcaccattaactcg	Protospacer
******.****************************

32. spacer 1.5|36595|35|CP020115|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 1, identity: 0.971

attgtcgggagggggatgatctcaccattaactcg	CRISPR spacer
attgtcgggagggggatgatctcaccattaacccg	Protospacer
********************************.**

33. spacer 1.5|36595|35|CP020115|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 1, identity: 0.971

attgtcgggagggggatgatctcaccattaactcg	CRISPR spacer
attgtcaggagggggatgatctcaccattaactcg	Protospacer
******.****************************

34. spacer 1.5|36595|35|CP020115|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 1, identity: 0.971

attgtcgggagggggatgatctcaccattaactcg	CRISPR spacer
attgtcgggagggggatgatctcaccattaacccg	Protospacer
********************************.**

35. spacer 1.5|36595|35|CP020115|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 1, identity: 0.971

attgtcgggagggggatgatctcaccattaactcg	CRISPR spacer
attgtcaggagggggatgatctcaccattaactcg	Protospacer
******.****************************

36. spacer 1.7|36718|35|CP020115|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 1, identity: 0.971

attgtcgggagggggatgatctcaccattaacccg	CRISPR spacer
attgtcgggagggggatgatctcaccattaactcg	Protospacer
********************************.**

37. spacer 1.9|36841|35|CP020115|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 1, identity: 0.971

attgtcaggagggggatgatctcaccattaactcg	CRISPR spacer
attgtcgggagggggatgatctcaccattaactcg	Protospacer
******.****************************

38. spacer 1.1|36349|35|CP020115|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 2, identity: 0.943

attgtcgggagggggatgatctcaccattaacccg	CRISPR spacer
attgtcaggagggggatgatctcaccattaactcg	Protospacer
******.*************************.**

39. spacer 1.1|36349|35|CP020115|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 2, identity: 0.943

attgtcgggagggggatgatctcaccattaacccg	CRISPR spacer
attgtcaggagggggatgatctcaccattaactcg	Protospacer
******.*************************.**

40. spacer 1.3|36472|35|CP020115|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 2, identity: 0.943

attgtcaggagggggatgatctcaccattaactcg	CRISPR spacer
attgtcgggagggggatgatctcaccattaacccg	Protospacer
******.*************************.**

41. spacer 1.3|36472|35|CP020115|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 2, identity: 0.943

attgtcaggagggggatgatctcaccattaactcg	CRISPR spacer
attgtcgggagggggatgatctcaccattaacccg	Protospacer
******.*************************.**

42. spacer 1.7|36718|35|CP020115|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 2, identity: 0.943

attgtcgggagggggatgatctcaccattaacccg	CRISPR spacer
attgtcaggagggggatgatctcaccattaactcg	Protospacer
******.*************************.**

43. spacer 1.7|36718|35|CP020115|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 2, identity: 0.943

attgtcgggagggggatgatctcaccattaacccg	CRISPR spacer
attgtcaggagggggatgatctcaccattaactcg	Protospacer
******.*************************.**

44. spacer 1.9|36841|35|CP020115|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 2, identity: 0.943

attgtcaggagggggatgatctcaccattaactcg	CRISPR spacer
attgtcgggagggggatgatctcaccattaacccg	Protospacer
******.*************************.**

45. spacer 1.9|36841|35|CP020115|CRT matches to NZ_CP020115 (Nodularia spumigena UHCC 0039 plasmid pUHCC0039a, complete sequence) position: , mismatch: 2, identity: 0.943

attgtcaggagggggatgatctcaccattaactcg	CRISPR spacer
attgtcgggagggggatgatctcaccattaacccg	Protospacer
******.*************************.**

46. spacer 2.2|45941|24|CP020115|CRISPRCasFinder matches to NZ_CP029452 (Sinorhizobium fredii CCBAU 25509 plasmid pSF25509b, complete sequence) position: , mismatch: 3, identity: 0.875

gctactcctgcggtgcgctcatca	CRISPR spacer
gttcctcctgcggtgcgctcatcg	Protospacer
*.* *******************.

47. spacer 2.2|45941|24|CP020115|CRISPRCasFinder matches to NZ_CP023071 (Sinorhizobium fredii CCBAU 83666 plasmid pSF83666b, complete sequence) position: , mismatch: 3, identity: 0.875

gctactcctgcggtgcgctcatca	CRISPR spacer
gttcctcctgcggtgcgctcatcg	Protospacer
*.* *******************.

48. spacer 2.2|45941|24|CP020115|CRISPRCasFinder matches to NZ_CP029232 (Sinorhizobium fredii CCBAU 45436 plasmid pSF45436b, complete sequence) position: , mismatch: 3, identity: 0.875

gctactcctgcggtgcgctcatca	CRISPR spacer
gttcctcctgcggtgcgctcatcg	Protospacer
*.* *******************.