CRISPRimmunity

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Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Self targeting spacer number	Target MGE spacer number	Prophage number	Anti-CRISPR protein number
CP028827	Vibrio cholerae strain N16961 chromosome 1, complete sequence	1 crisprs	cas3,RT,DEDDh,DinG,csx1,WYL,csa3	0	1	5	0
CP028828	Vibrio cholerae strain N16961 chromosome 2, complete sequence	0 crisprs	PD-DExK,csa3,cas3	0	0	0	0

Results visualization

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CRISPR-Cas detection and classification

Crispr_ID: CP028827_1

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

CP028827_1

2604035-2604278

Orphan

Consensus_repeat	Method
AAAATGTGCCTCCTTAGCTCAGTCGGTAGAGCAACGGACTGATAATCCG	PILER-CR

2 spacers

The CRISPR arrays of CP028827_1

>merge|CP028827|1|2604035-2604278|PILER-CR
TGTGCCGGCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCGCCAGTTCGATTCCGGCAGCCGGCACCACTTAAAATTTGCCTCGATAGCTCAGTCGGTAGAGCAGAGGATTGAAAATCCTCGTGTCGGTGGTTCGATTCCGCCTCGAGGCACCACTATTAAAAGTTGTGCCATAGACACAATAAAAATTGTTCCTCCTTAGCTCAGTCGGTAGAGCGACGGACTGTTAATCCG

>CP028827|1|1|2604035-2604278|PILER-CR
TGTGCCGGCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGG	TCGCCAGTTCGATTCCGGCAGCCGGCACCACTTAAAA
TTTGCCTCGATAGCTCAGTCGGTAGAGCAGAGGATTGAAAATCCTCGTG	TCGGTGGTTCGATTCCGCCTCGAGGCACCACTATTAAAAGTTGTGCCATAGACACAATAAAAAT
TGTTCCTCCTTAGCTCAGTCGGTAGAGCGACGGACTGTTAATCCG

Protein	Signature genes	Signature genes Name	Protein_function
CP028827.1\|AWA79424.1\|2615693_2616167_-\|hypothetical-protein	unknown	unknown	gnl\|CDD\|182143
CP028827.1\|AWA79419.1\|2611941_2612934_+\|4-hydroxythreonine-4-phosphate-dehydrogenase	unknown	unknown	gnl\|CDD\|234696
CP028827.1\|AWA79422.1\|2614225_2615035_+\|Bis(5'-nucleosyl)-tetraphosphatase-[symmetrical]	unknown	unknown	gnl\|CDD\|234673
CP028827.1\|AWA79421.1\|2613834_2614215_+\|Protein-ApaG	unknown	unknown	gnl\|CDD\|180098
CP028827.1\|AWA79405.1\|2595851_2596409_+\|hypothetical-protein	unknown	unknown	gnl\|CDD\|223833
CP028827.1\|AWA79412.1\|2601227_2602289_+\|Adenine-DNA-glycosylase	unknown	unknown	gnl\|CDD\|182805
CP028827.1\|AWA79415.1\|2604567_2606193_+\|Methyl-accepting-chemotaxis-protein-McpP	unknown	unknown	gnl\|CDD\|223910
CP028827.1\|AWA79411.1\|2600268_2600988_-\|tRNA-(guanine-N(7)-)-methyltransferase	unknown	unknown	gnl\|CDD\|234649
CP028827.1\|AWA79407.1\|2596744_2597176_+\|hypothetical-protein	unknown	unknown	gnl\|CDD\|379616
CP028827.1\|AWA79423.1\|2615183_2615681_-\|Dihydrofolate-reductase-type-3	unknown	unknown	gnl\|CDD\|365932
CP028827.1\|AWA79408.1\|2597245_2597848_+\|dITP/XTP-pyrophosphatase	unknown	unknown	gnl\|CDD\|234648
CP028827.1\|AWA79413.1\|2602302_2602575_+\|putative-Fe(2+)-trafficking-protein	unknown	unknown	gnl\|CDD\|180059
CP028827.1\|AWA79410.1\|2599237_2600158_-\|Glutaminase-2	unknown	unknown	gnl\|CDD\|234880
CP028827.1\|AWA79416.1\|2607239_2608094_-\|Co-chaperone-protein-DjlA	unknown	unknown	gnl\|CDD\|236512
CP028827.1\|AWA79418.1\|2610656_2611952_+\|Chaperone-SurA	unknown	unknown	gnl\|CDD\|236758
CP028827.1\|AWA79417.1\|2608252_2610604_+\|LPS-assembly-protein-LptD	unknown	unknown	gnl\|CDD\|235158
CP028827.1\|AWA79414.1\|2602676_2603804_+\|Membrane-bound-lytic-murein-transglycosylase-C	unknown	unknown	gnl\|CDD\|183271
CP028827.1\|AWA79420.1\|2612947_2613763_+\|Ribosomal-RNA-small-subunit-methyltransferase-A	unknown	unknown	gnl\|CDD\|234708
CP028827.1\|AWA79409.1\|2597841_2599017_+\|Oxygen-independent-coproporphyrinogen-III-oxidase-like-protein-YqeR	unknown	unknown	gnl\|CDD\|235550
CP028827.1\|AWA79406.1\|2596408_2596699_+\|hypothetical-protein	unknown	unknown	gnl\|CDD\|179931

Protein	Function_ID	Function_description	E-value
CP028827.1\|AWA79424.1\|2615693_2616167_-\|hypothetical-protein	gnl\|CDD\|182143	PRK09917, PRK09917, threonine/serine exporter.	7.45224e-67
CP028827.1\|AWA79419.1\|2611941_2612934_+\|4-hydroxythreonine-4-phosphate-dehydrogenase	gnl\|CDD\|234696	PRK00232, pdxA, 4-hydroxythreonine-4-phosphate dehydrogenase; Reviewed.	0
CP028827.1\|AWA79422.1\|2614225_2615035_+\|Bis(5'-nucleosyl)-tetraphosphatase-[symmetrical]	gnl\|CDD\|234673	PRK00166, apaH, symmetrical bis(5'-nucleosyl)-tetraphosphatase.	1.41419e-169
CP028827.1\|AWA79421.1\|2613834_2614215_+\|Protein-ApaG	gnl\|CDD\|180098	PRK05461, apaG, CO2+/MG2+ efflux protein ApaG; Reviewed.	1.16069e-74
CP028827.1\|AWA79405.1\|2595851_2596409_+\|hypothetical-protein	gnl\|CDD\|223833	COG0762, COG0762, Predicted integral membrane protein [Function unknown].	1.96316e-12
CP028827.1\|AWA79412.1\|2601227_2602289_+\|Adenine-DNA-glycosylase	gnl\|CDD\|182805	PRK10880, PRK10880, adenine DNA glycosylase.	0
CP028827.1\|AWA79415.1\|2604567_2606193_+\|Methyl-accepting-chemotaxis-protein-McpP	gnl\|CDD\|223910	COG0840, Tar, Methyl-accepting chemotaxis protein [Cell motility and secretion / Signal transduction mechanisms].	2.35326e-58
CP028827.1\|AWA79411.1\|2600268_2600988_-\|tRNA-(guanine-N(7)-)-methyltransferase	gnl\|CDD\|234649	PRK00121, trmB, tRNA (guanine-N(7)-)-methyltransferase; Reviewed.	6.01203e-111
CP028827.1\|AWA79407.1\|2596744_2597176_+\|hypothetical-protein	gnl\|CDD\|379616	pfam14467, DUF4426, Domain of unknown function (DUF4426). Members of this entry are found mostly in g-proteobacteria, especially in Vibrio. Strangely enough, there seems to be one eukaryotic homolog in Nematostella vectensis (NEMVEDRAFT_v1g226006), where the PA0388-like domain is fused with a domain homogous to the Methionine biosynthesis protein MetW (see below). In several Pseudomonas species, but also in Vibrio vulnificus and Azotobacter vinelandii PA0388 homologs are genomic neighbors of Nucleoside 5-triphosphatase RdgB (dHAPTP, dITP, XTP-specific) (EC 3.6.1.15) and Methionine biosynthesis protein MetW. On the other hand, in most Vibrio species it appears as a part of a conserved operon involved in possible response to stress.	6.80534e-67
CP028827.1\|AWA79423.1\|2615183_2615681_-\|Dihydrofolate-reductase-type-3	gnl\|CDD\|365932	pfam00186, DHFR_1, Dihydrofolate reductase.	1.40644e-92
CP028827.1\|AWA79408.1\|2597245_2597848_+\|dITP/XTP-pyrophosphatase	gnl\|CDD\|234648	PRK00120, PRK00120, dITP/XTP pyrophosphatase; Reviewed.	4.099e-127
CP028827.1\|AWA79413.1\|2602302_2602575_+\|putative-Fe(2+)-trafficking-protein	gnl\|CDD\|180059	PRK05408, PRK05408, oxidative damage protection protein; Provisional.	5.47988e-59
CP028827.1\|AWA79410.1\|2599237_2600158_-\|Glutaminase-2	gnl\|CDD\|234880	PRK00971, PRK00971, glutaminase; Provisional.	0
CP028827.1\|AWA79416.1\|2607239_2608094_-\|Co-chaperone-protein-DjlA	gnl\|CDD\|236512	PRK09430, djlA, co-chaperone DjlA.	1.60415e-153
CP028827.1\|AWA79418.1\|2610656_2611952_+\|Chaperone-SurA	gnl\|CDD\|236758	PRK10770, PRK10770, peptidyl-prolyl cis-trans isomerase SurA; Provisional.	9.94454e-157
CP028827.1\|AWA79417.1\|2608252_2610604_+\|LPS-assembly-protein-LptD	gnl\|CDD\|235158	PRK03761, PRK03761, LPS assembly outer membrane complex protein LptD; Provisional.	0
CP028827.1\|AWA79414.1\|2602676_2603804_+\|Membrane-bound-lytic-murein-transglycosylase-C	gnl\|CDD\|183271	PRK11671, mltC, membrane-bound lytic murein transglycosylase MltC.	9.42926e-157
CP028827.1\|AWA79420.1\|2612947_2613763_+\|Ribosomal-RNA-small-subunit-methyltransferase-A	gnl\|CDD\|234708	PRK00274, ksgA, 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA.	3.27746e-146
CP028827.1\|AWA79409.1\|2597841_2599017_+\|Oxygen-independent-coproporphyrinogen-III-oxidase-like-protein-YqeR	gnl\|CDD\|235550	PRK05660, PRK05660, radical SAM family heme chaperone HemW.	0
CP028827.1\|AWA79406.1\|2596408_2596699_+\|hypothetical-protein	gnl\|CDD\|179931	PRK05090, PRK05090, hypothetical protein; Validated.	2.12816e-59

>CP028827.1|AWA79414.1|2602676_2603804_+|Membrane-bound-lytic-murein-transglycosylase-C
MRKLVLCITALLLSGCSREFIEKIYDVDYEPTNRFAKNLAELPGQFQKDTAALDALINSFSGNIEKRWGRRELKIAGKNNYVKYIDNYLSRSEVNFTEGRIIVETVSPIDPKAHLRNAIITTLLTPDDPAHVDLFSSKDIELKGQPFLYQQVLDQDGQPIQWSWRANRYADYLIANHLKVKQVDFKKAYYVEIPMVKDQIDIRGYKYASIVRKASRKYDIPEDLIYAIIKTESSFNPYAVSWANAYGLMQVVPKTAGRDVFKLVKNRSGEPSPEYLFNPENNIDTGTAYFYILKNRYLKEVRHPTSLEYSMISAYNGGTGGVLSTFSSDRQRAMRDLNALQPNQVYWALTKKHPNAEARRYLEKVTKFKKEFNAG
>CP028827.1|AWA79413.1|2602302_2602575_+|putative-Fe(2+)-trafficking-protein
MARTVFCTRLQKEADGLDFQLYPGELGKRIFDNICKEAWAQWQTKQTMLINEKKLNMMDPEHRKLLEQEMVNFLFEGKEVHIEGYTPPAK
>CP028827.1|AWA79412.1|2601227_2602289_+|Adenine-DNA-glycosylase
MTPFAQAILTWYDAYGRKNLPWQQNKNAYRVWLSEIMLQQTQVATVIPYFERFLERFPTVHALAAAPQDEVLHFWTGLGYYARARNLHKAAQMVVSEYGGEFPTDLEQMNALPGVGRSTAAAVLSSVYKKPHAILDGNVKRTLARCFAVEGWPGQKSVENQLWHYAEMHTPKVDVDKYNQAMMDMGAMICTRSKPKCSLCPVESFCLAKQQGNPQEYPGKKPKTDKPVKATWFVMLYHDNAVWLEQRPQSGIWGGLYCFPQSEIANIQTTIDQRAIGDSTITSQKTLIAFRHTFSHYHLDITPILLQLSRKPDIVMEGSKGLWYNLSHPDEIGLAAPVKQLLHTLPFDIDSHI
>CP028827.1|AWA79411.1|2600268_2600988_-|tRNA-(guanine-N(7)-)-methyltransferase
MSEVITQEYTEDGKVLRRIRSFVRREGRLTKGQEAAMKECWPTMGIDYQPELLDWQQVFGNDNPVVLEIGFGMGASLVEMAKNAPEKNFLGIEVHSPGVGACLASAREAGVTNLRVMCHDAVEVFAHMIPDNSLHTLQLFFPDPWHKKRHHKRRIVQLEFAEMVRQKLMIGSGVFHMATDWENYAEHMVEVMNQAPGFANLATDGDYVPRPDERPLTKFEQRGHRLGHGVWDIKYQRTA
>CP028827.1|AWA79410.1|2599237_2600158_-|Glutaminase-2
MKPTADILASIIEEVRPLTSKGIVADYIPALAKVPSDKLGIAVFTNQGEVITAGDAQEGFSIQSISKVLSLTLAMGLYQPDELWSRVGKEPSGQAFNSLIQLEMEQGIPRNPFINAGAIVVCDMLQSRLSAPRQRLLEFVRQLSGEPQIAYDKVVAASEMMHSDRNAAIAYLMRSFGNFHNEVIPVLHNYFHACALKMSCVELAKTFSYLANKGVSVVTGETVITPTQSKQTNALLATCGLYDGAGEFAYRVGMPGKSGVGGGIIAVVPGEMTIAVWSPALDQSGNSLAGTRALELLAQRIGRSIF
>CP028827.1|AWA79409.1|2597841_2599017_+|Oxygen-independent-coproporphyrinogen-III-oxidase-like-protein-YqeR
MLTPPSLSLYIHIPWCVQKCPYCDFNSHAQKGEIPEQEYLDALLQDLDRDIERYQLKGDPRLLHSIFIGGGTPSLISAEGIARLLQGVAERIAFKPEIEITMEANPGTIEAQRFAGYRTAGVTRISIGVQSFEPEKLARLGRIHGQQEAVRAAQLAHQIGLNSFNLDLMHGLPDQTPEQALADLDQAIALNPPHLSWYQLTIEPNTLFYSKPPKLPDEDALWDIFELGHQKLSDAGYVQYEISGYSKPGYQCQHNLNYWRFGDYLGIGCGAHGKLSFADGRIVRTTKTKHPRGYLAALNNLAKAYLDSEQLVADQDKPFEFFMNRFRLIEPCPKADFTATTGLTIDVIRPTLDWALSEGYLSEDDQHWQITEKGKLFLNDLLEAFMADEEE
>CP028827.1|AWA79408.1|2597245_2597848_+|dITP/XTP-pyrophosphatase
MKKIVLATGNQGKVREMADLLSDFGFDVVAQSEFNVPEAAETGTTFIENAIIKARHAAQITGLPTIADDSGLEVDYLNGAPGIYSARYAGEHASDGDNLNKLLMAMQDVPDDQRSARFHCVLVLMRHADDPTPIVCHGKWEGKILTAPHGSNGFGYDPIFWVPEENCASAELEPVRKKQLSHRGKALQKLFKAIEEQRTC
>CP028827.1|AWA79407.1|2596744_2597176_+|hypothetical-protein
MRKWMMSLLLTLLALPASAEQQKTIKDIEVHYSAFNSTFLTPKVASSYQLTRNGYTAILNISVLDRASLGKPATEAKLTGHAKNLIGNLRELSFKQVKEGNAIYYLAEVPISNEEMLTFDIDVDAGLKGAGKLTFSQKFYTEQ
>CP028827.1|AWA79406.1|2596408_2596699_+|hypothetical-protein
MAAAWREGDDLLLRLYIQPKASRDSIVGLHGEELKVAITAPPIDGKANAHLSKYLAKLCKVAKGSVVVEKGELGRHKQVRILQPSQIPAEIAALIE
>CP028827.1|AWA79405.1|2595851_2596409_+|hypothetical-protein
MNSLSFLINTLFDLYIMVVILRIWLQAARADFYNPFSQFIVKATQPVVGPLRRVIPSIGSIDLATIVFAYVLCVLKFMALVLIASSGSVSFSADFLFLGLLSLIKAAGGLLFWVLLIRAILSWVSQGRSPIEYVFHQLTEPMLAPIRRIIPVMGGFDLSVLVLFIVLQFANFLMGDVIGPIWYQL
>CP028827.1|AWA79415.1|2604567_2606193_+|Methyl-accepting-chemotaxis-protein-McpP
MKLKTQAYLLSAIILAALLALTATGLWTLRVASNLDNKARVTELFNSAYSILTEVEKLAQEGKMSEPEAKALATRLMRNNLYKDNEYVYVADENMTFVATPLDPQLHDTSFHDFKDGKGNSVGRLIQDVLRHQSGKLVEYTWTQKQADGSIEEKLSIARKTPHWGWVVGTGIGFNEVNARFWSTAQWQLSLCVVIAVAILSLLLVAIRKILLIIGGEPNEVRSAVQAVAQGRIRREFVIKAPKESIYGAVQQMSSSLADLVAKLEQSMVALRSELAGAGTRAKSIAELTDSQQQSTAMIATAMTEMASSANQVADSARDTAFNTDQADQQSQHTQKLIHNTVSNIQGLATQLQTASTAVADLDLDVKNIAKVLDVIGDIAEQTNLLALNAAIEAARAGEQGRGFAVVADEVRNLAGRTQTSTKEIQQMIHNLQEGSRNAIQTIQICGQTSQSSVQESENAASALALIVSALESVSSMSHQIATAAAEQTQVSDDIARRINMIEESGSKLSRVVMESHNSTQTLTKLARELEQWAAHFEVTR
>CP028827.1|AWA79416.1|2607239_2608094_-|Co-chaperone-protein-DjlA
MHIFGKILGAFFGFLFGGPFGAIFGIFLGHQFDKARRLNQAGFQSGTFGAGPSQAERQEEFFKSAFSVMGHVAKAKGQVTKEEIQLATIMMDRMNLTLEQKRAAQDAFRDGKESDFPLEQVLERVKIATGGRFDLLQFFLELQVSSAFADGDVHPSERQVLHRIARGLGFSSEQLERRLRMQEAAFRFQQGGGFGGSQQQSHSGQQWQQPSSRHQLADAYEVLGVSESASAQEVKRAYRKLMNEHHPDKLMAKGLPPEMMNVAKEKSQQIQHAYELIRKEKGIK
>CP028827.1|AWA79417.1|2608252_2610604_+|LPS-assembly-protein-LptD
MSCFSRTFLAASISAALFAPQIQAEASVDDNRAQLPNGEQCLVNQPEPTNPGQQPINVEADKLEAINGQKATYSGNVVVVQGKKRIAADNVTLHQQENVVVAEGNVQFSDGEIKTHSTKATNHLNTDEMTLENTRYQFLCEPGRGEAVYVSKTGKAVYEIEDGSITSCPDGDNAWRMRASSIDVDQNEEIATFYNPRLEVQNVPVFYLPYLTVPIGDTRKTGFLYPTASYGSRNGYSFEVPIYWNLAPQYDLETTFNYMQKRGTQLNSVFRYLTDFGAGQIKSEYLADDQLHTELGDRWAFQYEHNGIFQQAWKFEIDYSKVSDINYFSDLDSGVGNREDGQLIQEGRATYRSDNWDSALLVRDFQLLTKDTTSTNLPYRLMPQLSYNYYAPETMKYLDLDLVSHVSRFETDARGKPSATRVHIEPGLKIPFSNTWGNWTTEARVLGTYYQQDLDKTTDAKLEESVTRVIPEIRSVAGIVLERDTVLLDDYTQTLEPKIQYLYVPEKYQDNIGLYDSTLLQTDYYGLFRSRKYSGVDRIESANQVSYGASTRFFDSNYKERLNIAFGQIFYLDSKLNPSNKNPDSTSDKTSYSAWAVEMDFNFADYLFYHGGIQYDIDSQAVQLGNSTLEYRVASGYIQANYRYVAKDYIRNTVGDSITNIDDITRDGISQAGILAGYQLSRKWSASGQYYYDLTTDEALEWLANLTYTSDCWYVGFTYSNQLKSWNGNFVTDPYATPIYENNFSFNIGIIGFGTSIGAGSSMTGVDSAGNSLGYGRPFFLNN
>CP028827.1|AWA79418.1|2610656_2611952_+|Chaperone-SurA
MKLWKPTLISVLSALTLFNAHAEPKQLDSVAVIVNSGVILQSDVDSALKTIKANAKQNKQPLPQETVLREQVLEKLIIDTLQQQEADRIGVKIDDNRLNEAIKEIAKNNQQTQEQLIASVAQEGLTYPEFREQVRKEMAASDARNALVRRRINILPAEVDTLAELLAQETDATVQYKISHIQLRVDDGQDKSTAETLANKLVNDLRNGADFAQMAYAYSKGPKALQGGDWGWMRKEEMPTIFADQIKMQNKGSIIGPFRSGVGFHILKIDDVKGLETVAVTEVNARHILIKPTIILSDEGAQKQLNEFVQRIKNGEVTFAELAQQYSQDPGSAAQKGELGYQTPDLYVPEFKHQIETLPVGQISEPFKTVHGWHIVEVLDRREVDRTDSALKNKAYRILFNRKFNEEASAWLQELRASAFVEVLKDEKDEQ
>CP028827.1|AWA79419.1|2611941_2612934_+|4-hydroxythreonine-4-phosphate-dehydrogenase
MSSKRIIVTAGEPAGIGPDLVLALSAQDWPHQLVVCADKALLAQRATQLGIQVKLLDYQRDNPVQAQQAGTLLVEHIPLAEPVVAGQLNPANGHYVLKTLERAAKGCMNGEFDAIVTGPVHKGVINRAGVAFSGHTEFFAEQSKTPLVVMMLATEGLRTALVTTHLPLAEVPQAITCERLEQIVHILHKDLVEKFAIAEPKIYVCGLNPHAGEDGVLGMDEIETITPTLQRLREQYGMQLVGPLPADTIFSEKYLQQADAVLGMYHDQVLPVLKYKGFGRSVNITLGLPFIRTSVDHGTALDLAGTGQADAGSFWTALAYAIELVDKKAQ
>CP028827.1|AWA79420.1|2612947_2613763_+|Ribosomal-RNA-small-subunit-methyltransferase-A
MRNDVHLGHKARKRFGQNFLNDPYIIDGIVSAINPKPGQNLVEIGPGLGAITEPVGREVDKFTVIELDRDLAERLRNHPELASKLTIHEGDAMRFDFKQLVKPNNKLRVFGNLPYNISTPLMFHLFEFHRDIQDMHFMLQKEVVNRLAAGPGTKAYGRLTVMAQYYCKVVPVLEVPPSAFVPPPKVDSAVVRLVPYEDLPHPATSLEWLDRVVREGFNQRRKTVRNCYKGLAEPETLETLGINPGMRPENLTLAQFVALANWLDATHKTHA
>CP028827.1|AWA79421.1|2613834_2614215_+|Protein-ApaG
MDVSLPCIKIQVQTRYIEEQSNPEYQRFVFAYLITIKNLSSQTVQLMSRRWLITDADGKQTVVEGDGVVGEQPRIKANDEYTYSSGTALDTPVGVMQGQYLMIDEQGESFTVEIEPFRLAVPHVLN
>CP028827.1|AWA79422.1|2614225_2615035_+|Bis(5'-nucleosyl)-tetraphosphatase-[symmetrical]
MANYIVGDIQGCFDELQQLLKQAEFNSQLDTLWFAGDLVARGPKSLETLRFVYQLGDAARVVLGNHDLHLLSVALGHHSAKRRDQTQAVLDAPDAAPLLDWLRQQPLLAEHQEFVLCHAGISPQWDLATARQAAQEVESVLRSPEWSTLIEQMYSDQPDAWHPTLQGIDRLRYIVNAFTRMRFCFPDGRLDMQCKLPPKEVTDGSLLPWFQLPQRIALEKTVIFGHWAALEGYVSETVIGLDTGCVWGGTLTMLRWEDKHYFSQAALPA
>CP028827.1|AWA79423.1|2615183_2615681_-|Dihydrofolate-reductase-type-3
MMISMIAAMADQRIIGKDNQMPWHLPADFAWFKRCTLGKPVVMGRKTYQSIGRPLPGRHNIVISRDASLQIEGVDVVTSIEAALAKAGEVDEVMIIGGGSLYAACLPMAHKLYITEIHAKLDGDTQFPEWGSDWLERSREHYPADEKNAYGMDFVIFERQYPLPT
>CP028827.1|AWA79424.1|2615693_2616167_-|hypothetical-protein
MLSWDLFFGLLNDMFFAAIPAVGFALVFNVPVPALKYCALGGALGHGSRYLMMHFGVPIEWASFFAATLVGMVGVYWSRRFLAHPKVFTVAALIPMVPGVFAFKAMIALVEINHVGFSPELMEALLENFLKAMFIIAGLAVGLAVPGLLFYRRKPII

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Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity
CP028827_1	1.1\|2604084\|37\|CP028827\|PILER-CR	2604084-2604120	37	NC_049942	Escherichia phage JLK-2012, complete sequence	23524-23560	4	0.892
CP028827_1	1.1\|2604084\|37\|CP028827\|PILER-CR	2604084-2604120	37	NC_021742	Serratia liquefaciens ATCC 27592 plasmid unnamed, complete sequence	35428-35464	4	0.892

1. spacer 1.1|2604084|37|CP028827|PILER-CR matches to NC_049942 (Escherichia phage JLK-2012, complete sequence) position: , mismatch: 4, identity: 0.892

taggtcgccagttcgattccggcagccggcaccactt	CRISPR spacer
caggtcgccagttcgattccggtagccggcaccatat	Protospacer
.*********************.***********. *

2. spacer 1.1|2604084|37|CP028827|PILER-CR matches to NC_021742 (Serratia liquefaciens ATCC 27592 plasmid unnamed, complete sequence) position: , mismatch: 4, identity: 0.892

taggtcgccagttcgattccggcagccggcaccactt	CRISPR spacer
taggtcaccagttcgattccggtagccggcaccaatc	Protospacer
******.***************.*********** *.

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

653003 : 659620

Staphylococcus_phage(66.67%)

The bacterium proteins that are colored denote the protein is present at specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin' and 'tRNA')

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AWA77713.1\|653003_654137_+	Glutamate 5-kinase	A0A1X9I5D0	Streptococcus_phage	1.4e-64	40.4
AWA77714.1\|654148_655399_+	Gamma-glutamyl phosphate reductase	A0A1X9I5D4	Streptococcus_phage	5.0e-100	49.5
AWA77715.1\|655498_655948_+	Transcriptional repressor NrdR	NA	NA	NA	NA
AWA77716.1\|655973_657077_+	Riboflavin biosynthesis protein RibD	A0A2H4PQS8	Staphylococcus_phage	1.3e-43	33.1
AWA77717.1\|657081_657735_+	Riboflavin synthase	A0A2H4PQS5	Staphylococcus_phage	1.2e-31	38.2
AWA77718.1\|657775_658885_+	Riboflavin biosynthesis protein RibBA	A0A2H4PQS2	Staphylococcus_phage	6.7e-64	39.8
AWA77719.1\|659149_659620_+	6,7-dimethyl-8-ribityllumazine synthase	A0A2H4PQS3	Staphylococcus_phage	6.8e-34	48.7

DBSCAN-SWA_2

1139703 : 1146603

Vibrio_phage(71.43%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AWA78157.1\|1139703_1139970_+	hypothetical protein	M1Q554	Vibrio_phage	1.4e-07	33.8
AWA78158.1\|1140371_1140746_+	hypothetical protein	NA	NA	NA	NA
AWA78159.1\|1140732_1141053_+	hypothetical protein	NA	NA	NA	NA
AWA78160.1\|1141071_1141650_+	hypothetical protein	NA	NA	NA	NA
AWA78161.1\|1141710_1142082_+	hypothetical protein	NA	NA	NA	NA
AWA78162.1\|1142130_1142490_+	hypothetical protein	A0A2I7S9E0	Vibrio_phage	3.7e-40	67.8
AWA78163.1\|1142624_1143665_+	hypothetical protein	A0A2I7S9D9	Vibrio_phage	2.6e-126	72.5
AWA78164.1\|1143707_1144016_+	hypothetical protein	Q716C1	Shigella_phage	7.1e-32	76.3
AWA78165.1\|1144258_1144885_+	hypothetical protein	Q716C2	Shigella_phage	1.1e-92	72.9
AWA78166.1\|1145010_1145577_+	hypothetical protein	M4MHE6	Vibrio_phage	7.5e-11	31.4
AWA78167.1\|1145672_1145810_-	hypothetical protein	NA	NA	NA	NA
AWA78168.1\|1145806_1146283_-	hypothetical protein	NA	NA	NA	NA
AWA78169.1\|1146426_1146603_+	hypothetical protein	A0A1V0E8E5	Vibrio_phage	2.8e-09	51.9

DBSCAN-SWA_3

1503383 : 1538718

Satellite_phage(43.48%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AWA78462.1\|1503383_1503551_+	hypothetical protein	E3U9J0	Vibrio_phage	2.3e-13	100.0
AWA78463.1\|1503543_1503711_+	hypothetical protein	NA	NA	NA	NA
AWA78464.1\|1504749_1505301_-	hypothetical protein	NA	NA	NA	NA
AWA78465.1\|1505477_1507046_+	hypothetical protein	A7BJY2	Enterobacteria_phage	2.7e-58	32.3
AWA78466.1\|1507050_1507335_+	hypothetical protein	NA	NA	NA	NA
AWA78467.1\|1507464_1508157_+	hypothetical protein	NA	NA	NA	NA
AWA78468.1\|1508234_1508402_+	hypothetical protein	E3U9J0	Vibrio_phage	2.3e-13	100.0
AWA78469.1\|1508394_1508979_+	hypothetical protein	E3U9I9	Vibrio_phage	6.4e-114	100.0
AWA78470.1\|1509468_1509807_-	hypothetical protein	U5TNA1	Satellite_phage	7.3e-54	100.0
AWA78471.1\|1509932_1511012_+	hypothetical protein	U5TQR3	Satellite_phage	2.2e-213	100.0
AWA78472.1\|1510992_1511373_+	hypothetical protein	A0A0N9HE73	Vibrio_phage	1.4e-69	100.0
AWA78473.1\|1511372_1511516_+	hypothetical protein	NA	NA	NA	NA
AWA78474.1\|1511508_1511757_+	hypothetical protein	A0A0N7F140	Vibrio_phage	8.8e-33	100.0
AWA78475.1\|1511863_1513051_+	hypothetical protein	A0A142I701	Vibrio_phage	1.4e-200	100.0
AWA78476.1\|1513056_1513341_+	hypothetical protein	A0A0F6YNQ7	Vibrio_phage	4.0e-45	100.0
AWA78477.1\|1513337_1514537_+	Zona occludens toxin	A0A142I6Z1	Vibrio_phage	5.3e-240	100.0
AWA78478.1\|1514635_1515412_+	Cholera enterotoxin subunit A	A0A142I6Y0	Vibrio_phage	1.3e-151	100.0
AWA78479.1\|1515408_1515783_+	Cholera enterotoxin subunit B	Q77DH7	Vibrio_virus	7.8e-65	100.0
AWA78480.1\|1516384_1516723_-	hypothetical protein	U5TNA1	Satellite_phage	7.3e-54	100.0
AWA78481.1\|1516848_1517928_+	hypothetical protein	U5TQR3	Satellite_phage	2.2e-213	100.0
AWA78482.1\|1517908_1518280_+	hypothetical protein	U5TMH5	Satellite_phage	3.3e-68	100.0
AWA78483.1\|1518373_1518598_+	hypothetical protein	U5TMI6	Satellite_phage	1.1e-34	100.0
AWA78484.1\|1519109_1519448_-	hypothetical protein	U5TNA1	Satellite_phage	7.3e-54	100.0
AWA78485.1\|1519573_1520653_+	hypothetical protein	U5TQR3	Satellite_phage	2.2e-213	100.0
AWA78486.1\|1520633_1521005_+	hypothetical protein	U5TMH5	Satellite_phage	3.3e-68	100.0
AWA78487.1\|1521098_1521323_+	hypothetical protein	U5TMI6	Satellite_phage	1.1e-34	100.0
AWA78488.1\|1521690_1535328_-	Multifunctional-autoprocessing repeats-in-toxin	B3Y8K3	Vibrio_virus	8.4e-07	100.0
AWA78489.1\|1535351_1535747_-	Leukotoxin-activating lysine-acyltransferase LtxC	NA	NA	NA	NA
AWA78490.1\|1535838_1536186_-	hypothetical protein	NA	NA	NA	NA
AWA78491.1\|1536609_1538718_+	Leukotoxin export ATP-binding protein LtxB	W8CYL7	Bacillus_phage	4.9e-39	27.1

DBSCAN-SWA_4

2277089 : 2284282

Anguillid_herpesvirus(16.67%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AWA79130.1\|2277089_2277518_-	Nucleoside diphosphate kinase	D2E8E1	Anguillid_herpesvirus	1.0e-20	38.4
AWA79131.1\|2277721_2279011_-	Peptidase B	Q6GYZ8	Mycoplasma_phage	1.7e-34	34.3
AWA79132.1\|2279230_2279425_-	hypothetical protein	NA	NA	NA	NA
AWA79133.1\|2279473_2279812_-	2Fe-2S ferredoxin	NA	NA	NA	NA
AWA79134.1\|2279825_2281676_-	Chaperone protein HscA	A0A0N9QXN8	Chrysochromulina_ericina_virus	5.3e-106	41.1
AWA79135.1\|2281699_2282215_-	Co-chaperone protein HscB	NA	NA	NA	NA
AWA79136.1\|2282263_2282587_-	Iron-binding protein IscA	A0A2H4N7N5	Lake_Baikal_phage	3.0e-25	50.5
AWA79137.1\|2282647_2283031_-	Iron-sulfur cluster assembly scaffold protein IscU	A0A218MKD1	uncultured_virus	5.4e-53	78.2
AWA79138.1\|2283067_2284282_-	Cysteine desulfurase IscS	A0A0H3TPH2	Faustovirus	1.1e-32	30.5

DBSCAN-SWA_5

2515910 : 2523134

uncultured_Mediterranean_phage(33.33%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AWA79330.1\|2515910_2516798_+	Cysteine synthase B	A0A1X9I5K7	Streptococcus_phage	4.1e-56	41.1
AWA79331.1\|2517068_2519654_+	DNA mismatch repair protein MutS	A0A1V0SC35	Catovirus	2.2e-33	23.0
AWA79332.1\|2519746_2520754_-	RNA polymerase sigma factor RpoS	F4YCU2	Synechococcus_phage	6.0e-35	34.2
AWA79333.1\|2520827_2521763_-	Murein hydrolase activator NlpD	D7RWE0	Brochothrix_phage	7.8e-05	34.7
AWA79334.1\|2521762_2522389_-	Protein-L-isoaspartate O-methyltransferase	A0A1B1IU40	uncultured_Mediterranean_phage	2.0e-36	52.1
AWA79335.1\|2522381_2523134_-	5'-nucleotidase SurE	A0A1B1ITZ2	uncultured_Mediterranean_phage	8.0e-69	53.3

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage