CRISPRimmunity

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Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Self targeting spacer number	Prophage number
CP026738	Bacillus megaterium strain YC4-R4 plasmid unnamed2	0 crisprs	csa3	0	0
CP026737	Bacillus megaterium strain YC4-R4 plasmid unnamed1	0 crisprs	NA	0	0
CP026740	Bacillus megaterium strain YC4-R4 plasmid unnamed4	0 crisprs	NA	0	1
CP026736	Bacillus megaterium strain YC4-R4 chromosome, complete genome	2 crisprs	cas3,DEDDh,DinG,csa3,WYL	2	4
CP026741	Bacillus megaterium strain YC4-R4 plasmid unnamed5	0 crisprs	NA	0	0
CP026739	Bacillus megaterium strain YC4-R4 plasmid unnamed3	0 crisprs	NA	0	0

Results visualization

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CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

6871 : 84121

Bacillus_phage(15.38%)

coat,integrase

attL 23653:23681|attR 90659:90687

The bacterium proteins that are colored denote the protein is present at specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin' and 'tRNA')

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AWD68739.1\|6871_7804_-\|integrase	integrase	NA	NA	NA	NA
AWD68740.1\|7982_9371_-	replication initiation protein	A0A1V0E006	Clostridioides_phage	1.2e-09	28.3
AWD68741.1\|10363_11257_+	transcriptional regulator	Q2I8D8	Bacillus_phage	9.7e-21	29.9
AWD68742.1\|11433_25245_+	hypothetical protein	A0A2I2L613	Orpheovirus	2.7e-05	25.0
23653:23681	attL	NA	NA	NA	NA
AWD68743.1\|25397_25925_+	adenylate kinase DNA topology modulator	NA	NA	NA	NA
AWD68744.1\|26345_26834_-\|coat	spore coat protein X	NA	NA	NA	NA
AWD68745.1\|26942_27644_-	peptidase	NA	NA	NA	NA
AWD68746.1\|27845_28250_-	DUF4183 domain-containing protein	NA	NA	NA	NA
AWD68747.1\|28568_28853_+	DUF4183 domain-containing protein	NA	NA	NA	NA
AWD68748.1\|28938_29508_-	collagen-like protein	NA	NA	NA	NA
AWD68749.1\|30106_30583_+	hypothetical protein	NA	NA	NA	NA
AWD68750.1\|31011_31524_+	exosporium protein D	NA	NA	NA	NA
AWD68751.1\|31693_32194_-	hypothetical protein	NA	NA	NA	NA
AWD68752.1\|32425_33481_-	hypothetical protein	NA	NA	NA	NA
AWD68753.1\|33486_34662_-	carbamoyl-phosphate synthase	NA	NA	NA	NA
AWD68754.1\|34665_35970_-	glycosyltransferase family 1 protein	NA	NA	NA	NA
AWD68755.1\|35966_36623_-	PIG-L domain-containing protein	A0A2D2W2P2	Stenotrophomonas_phage	5.6e-10	24.5
AWD68756.1\|37182_37926_+	hypothetical protein	NA	NA	NA	NA
AWD68757.1\|38068_38890_-	YncE family protein	NA	NA	NA	NA
AWD68758.1\|39186_39402_+\|coat	spore coat protein	NA	NA	NA	NA
AWD68759.1\|39540_40101_+\|coat	spore coat protein X	NA	NA	NA	NA
AWD68760.1\|40126_40555_+\|coat	spore coat protein	NA	NA	NA	NA
AWD68761.1\|40761_41196_-	hypothetical protein	NA	NA	NA	NA
AWD68762.1\|41439_42756_+	glycosyltransferase family 2 protein	NA	NA	NA	NA
AWD68763.1\|42757_42976_-	hypothetical protein	NA	NA	NA	NA
AWD68764.1\|42991_43516_-	hypothetical protein	NA	NA	NA	NA
AWD68765.1\|43661_44378_-	glycosyl transferase family 2	NA	NA	NA	NA
AWD68766.1\|44532_45615_+	glycosyltransferase family 1 protein	NA	NA	NA	NA
AWD68767.1\|45649_46894_-	glycosyltransferase family 1 protein	NA	NA	NA	NA
AWD68768.1\|47465_48197_-	glycosyl transferase	NA	NA	NA	NA
AWD68769.1\|48473_49364_-	UDP-2-acetamido-2,6-dideoxy-hexulose 4-reductase	A0A222YY99	Synechococcus_phage	1.4e-19	26.5
AWD68770.1\|49579_50803_+	glycosyltransferase family 1 protein	NA	NA	NA	NA
AWD68771.1\|50917_51835_+	UDP-glucose 4-epimerase	A0A0E3I8S3	Synechococcus_phage	1.1e-38	32.6
AWD68772.1\|51972_52596_+	peptidase	A0A1V0SAM1	Catovirus	6.1e-06	32.1
AWD68773.1\|52905_53094_+	hypothetical protein	NA	NA	NA	NA
AWD68774.1\|53298_54123_-	SDR family NAD(P)-dependent oxidoreductase	A0A2P1ELT6	Moumouvirus	4.6e-33	35.9
AWD68775.1\|54123_55110_-	UDP-N-acetylglucosamine 4,6-dehydratase	A0A1V0SAI8	Catovirus	1.3e-45	33.5
AWD68776.1\|55102_56194_-	UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing)	A0A2P1ELS7	Moumouvirus	8.9e-93	47.5
AWD68777.1\|56597_57293_-	peptidase	NA	NA	NA	NA
AWD68778.1\|57433_58114_-	peptidase	NA	NA	NA	NA
AWD68779.1\|58326_59574_-	peptidase S8	A0A1B0T6A2	Bacillus_phage	1.0e-100	52.9
AWD68780.1\|60685_60874_-	hypothetical protein	NA	NA	NA	NA
AWD68781.1\|61082_61325_-	hypothetical protein	NA	NA	NA	NA
AWD68782.1\|61397_61679_-	hypothetical protein	NA	NA	NA	NA
AWD68783.1\|62173_63514_-	FAD-binding oxidoreductase	A0A0B5JBT9	Pandoravirus	1.1e-39	27.3
AWD68784.1\|71965_72577_+	recombinase family protein	M9Q1K0	Clostridium_phage	1.4e-15	30.4
AWD68785.1\|72757_72955_+	IDEAL domain-containing protein	NA	NA	NA	NA
AWD68786.1\|73033_73438_-	hypothetical protein	NA	NA	NA	NA
AWD68787.1\|73505_74354_-	hypothetical protein	NA	NA	NA	NA
AWD68788.1\|74541_75276_+	hypothetical protein	NA	NA	NA	NA
AWD68789.1\|75463_76123_+	peptidase	NA	NA	NA	NA
AWD68790.1\|79070_79328_+	hypothetical protein	NA	NA	NA	NA
AWD68802.1\|79821_80154_+	hypothetical protein	NA	NA	NA	NA
AWD68791.1\|80293_80965_-	hypothetical protein	NA	NA	NA	NA
AWD68792.1\|81577_82153_-	DUF3967 domain-containing protein	NA	NA	NA	NA
AWD68793.1\|83188_84121_-\|integrase	integrase	NA	NA	NA	NA
90659:90687	attR	NA	NA	NA	NA

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

2. CP026736

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CRISPR-Cas detection and classification

Crispr_ID: CP026736_2

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

CP026736_2

4442242-4442391

Orphan

Consensus_repeat	Method
CTTTTGCCGTTTTTGACGTACTACT	CRISPRCasFinder

2 spacers

The CRISPR arrays of CP026736_2

>merge|CP026736|2|4442242-4442391|CRISPRCasFinder
GTTTTGTCATTTTTAACGTACTACACAAAAGCTTTTGCCGTTTTTGACGTACTACTCCGCTACTTTTGCCGTTTTCTGCGTACTAATTACTGTCTTTTGCTGTTTTTGACGTACTACTTTGCTTCTTTTGCTGTTTTTGACGTACTAAAT

>CP026736|2|1|4442242-4442391|CRISPRCasFinder
GTTTTGTCATTTTTAACGTACTACA	CAAAAGCTTTTGCCGTTTTTGACGTACTACTCCGCTA
CTTTTGCCGTTTTCTGCGTACTAAT	TACTGTCTTTTGCTGTTTTTGACGTACTACTTTGCTT
CTTTTGCTGTTTTTGACGTACTAAAT

Protein	Signature genes	Signature genes Name	Protein_function
CP026736.1\|AWD67676.1\|4433483_4434038_-\|N-acetyltransferase	unknown	unknown	gnl\|CDD\|366181
CP026736.1\|AWD67690.1\|4449899_4451132_-\|aminopeptidase	unknown	unknown	gnl\|CDD\|376723
CP026736.1\|AWD67677.1\|4434407_4435232_-\|thermonuclease	unknown	unknown	gnl\|CDD\|214615
CP026736.1\|AWD67673.1\|4430969_4431383_-\|DUF4395-domain-containing-protein	unknown	unknown	gnl\|CDD\|379566
CP026736.1\|AWD67679.1\|4436543_4438046_-\|malate:quinone-oxidoreductase	unknown	unknown	gnl\|CDD\|377591
CP026736.1\|AWD67684.1\|4444988_4445585_+\|mechanosensitive-ion-channel-protein-MscS	unknown	unknown	gnl\|CDD\|366370
CP026736.1\|AWD67680.1\|4438166_4438991_-\|kinase/pyrophosphorylase	unknown	unknown	gnl\|CDD\|377080
CP026736.1\|AWD67683.1\|4443122_4444145_+\|hypothetical-protein	unknown	unknown	unknown
CP026736.1\|AWD67686.1\|4446293_4447307_-\|hypothetical-protein	unknown	unknown	gnl\|CDD\|226728
CP026736.1\|AWD67674.1\|4431695_4432526_-\|YitT-family-protein	unknown	unknown	gnl\|CDD\|224203
CP026736.1\|AWD67675.1\|4432725_4433286_-\|hypothetical-protein	unknown	unknown	gnl\|CDD\|276809
CP026736.1\|AWD67678.1\|4435632_4436292_-\|2OG-Fe(II)-oxygenase	unknown	unknown	gnl\|CDD\|177683
CP026736.1\|AWD67681.1\|4439494_4440376_-\|HAD-family-phosphatase	unknown	unknown	gnl\|CDD\|223635
CP026736.1\|AWD67687.1\|4447379_4447799_-\|hypothetical-protein	unknown	unknown	unknown
CP026736.1\|AWD67682.1\|4440689_4442153_+\|hypothetical-protein	unknown	unknown	unknown
CP026736.1\|AWD67685.1\|4445587_4446166_-\|HAD-family-hydrolase	unknown	unknown	gnl\|CDD\|319825
CP026736.1\|AWD67689.1\|4448943_4449780_-\|hypothetical-protein	unknown	unknown	unknown
CP026736.1\|AWD67692.1\|4452122_4452899_-\|DUF2437-domain-containing-protein	unknown	unknown	gnl\|CDD\|223257
CP026736.1\|AWD67688.1\|4448206_4448698_+\|hypothetical-protein	unknown	unknown	unknown
CP026736.1\|AWD67691.1\|4451275_4452070_-\|IclR-family-transcriptional-regulator	unknown	unknown	gnl\|CDD\|224332

Protein	Function_ID	Function_description	E-value
CP026736.1\|AWD67676.1\|4433483_4434038_-\|N-acetyltransferase	gnl\|CDD\|366181	pfam00583, Acetyltransf_1, Acetyltransferase (GNAT) family. This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.	1.1822e-12
CP026736.1\|AWD67690.1\|4449899_4451132_-\|aminopeptidase	gnl\|CDD\|376723	pfam02073, Peptidase_M29, Thermophilic metalloprotease (M29).	0
CP026736.1\|AWD67677.1\|4434407_4435232_-\|thermonuclease	gnl\|CDD\|214615	smart00318, SNc, Staphylococcal nuclease homologues.	4.92218e-39
CP026736.1\|AWD67685.1\|4445587_4446166_-\|HAD-family-hydrolase	gnl\|CDD\|319825	cd07523, HAD_YsbA-like, uncharacterized family of the haloacid dehalogenase-like superfamily, similar to the uncharacterized Lactococcus lactis YsbA. The specific function of Lactococcus lactis YsbA is unknown. Members of this family belong to the haloacid dehalogenase-like (HAD) hydrolases, a large superfamily of diverse enzymes that catalyze carbon or phosphoryl group transfer reactions on a range of substrates, using an active site aspartate in nucleophilic catalysis. Members of this superfamily include 2-L-haloalkanoic acid dehalogenase, azetidine hydrolase, phosphonoacetaldehyde hydrolase, phosphoserine phosphatase, phosphomannomutase, P-type ATPases and many others. HAD hydrolases are found in all three kingdoms of life, and most genomes are predicted to contain multiple HAD-like proteins. Members possess a highly conserved alpha/beta core domain, and many also possess a small cap domain, the fold and function of which is variable. HAD hydrolases are sometimes referred to as belonging to the DDDD superfamily of phosphohydrolases.	1.54713e-42
CP026736.1\|AWD67679.1\|4436543_4438046_-\|malate:quinone-oxidoreductase	gnl\|CDD\|377591	pfam06039, Mqo, Malate:quinone oxidoreductase (Mqo). This family consists of several bacterial Malate:quinone oxidoreductase (Mqo) proteins (EC:1.1.99.16). Mqo takes part in the citric acid cycle. It oxidizes L-malate to oxaloacetate and donates electrons to ubiquinone-1 and other artificial acceptors or, via the electron transfer chain, to oxygen. NAD is not an acceptor and the natural direct acceptor for the enzyme is most likely a quinone. The enzyme is therefore called malate:quinone oxidoreductase, abbreviated to Mqo. Mqo is a peripheral membrane protein and can be released from the membrane by addition of chelators.	0
CP026736.1\|AWD67680.1\|4438166_4438991_-\|kinase/pyrophosphorylase	gnl\|CDD\|377080	pfam03618, Kinase-PPPase, Kinase/pyrophosphorylase. This family of regulatory proteins has ADP-dependent kinase and inorganic phosphate-dependent pyrophosphorylase activity.	3.04958e-145
CP026736.1\|AWD67684.1\|4444988_4445585_+\|mechanosensitive-ion-channel-protein-MscS	gnl\|CDD\|366370	pfam00924, MS_channel, Mechanosensitive ion channel. Two members of this protein family of M. jannaschii have been functionally characterized. Both proteins form mechanosensitive (MS) ion channels upon reconstitution into liposomes and functional examination by the patch-clamp technique. Therefore this family are likely to also be MS channel proteins.	1.0926e-24
CP026736.1\|AWD67674.1\|4431695_4432526_-\|YitT-family-protein	gnl\|CDD\|224203	COG1284, COG1284, Uncharacterized conserved protein [Function unknown].	1.74103e-71
CP026736.1\|AWD67686.1\|4446293_4447307_-\|hypothetical-protein	gnl\|CDD\|226728	COG4278, COG4278, Uncharacterized conserved protein [Function unknown].	2.9051e-08
CP026736.1\|AWD67673.1\|4430969_4431383_-\|DUF4395-domain-containing-protein	gnl\|CDD\|379566	pfam14340, DUF4395, Domain of unknown function (DUF4395). This family of proteins is functionally uncharacterized. This family of proteins is found in bacteria and eukaryotes. Proteins in this family are typically between 142 and 168 amino acids in length. There are two completely conserved C residues that may be functionally important.	7.9414e-22
CP026736.1\|AWD67675.1\|4432725_4433286_-\|hypothetical-protein	gnl\|CDD\|276809	sd00006, TPR, Tetratricopeptide repeat. The Tetratricopeptide repeat (TPR) typically contains 34 amino acids and is found in a variety of organisms including bacteria, cyanobacteria, yeast, fungi, plants, and humans. It is present in a variety of proteins including those involved in chaperone, cell-cycle, transcription, and protein transport complexes. The number of TPR motifs varies among proteins. Those containing 5-6 tandem repeats generate a right-handed helical structure with an amphipathic channel that is thought to accommodate an alpha-helix of a target protein. It has been proposed that TPR proteins preferentially interact with WD-40 repeat proteins, but in many instances several TPR-proteins seem to aggregate to multi-protein complexes.	0.00183094
CP026736.1\|AWD67681.1\|4439494_4440376_-\|HAD-family-phosphatase	gnl\|CDD\|223635	COG0561, Cof, Predicted hydrolases of the HAD superfamily [General function prediction only].	2.29079e-58
CP026736.1\|AWD67678.1\|4435632_4436292_-\|2OG-Fe(II)-oxygenase	gnl\|CDD\|177683	PLN00052, PLN00052, prolyl 4-hydroxylase; Provisional.	9.3072e-41
CP026736.1\|AWD67692.1\|4452122_4452899_-\|DUF2437-domain-containing-protein	gnl\|CDD\|223257	COG0179, MhpD, 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) [Secondary metabolites biosynthesis, transport, and catabolism].	9.01675e-73
CP026736.1\|AWD67691.1\|4451275_4452070_-\|IclR-family-transcriptional-regulator	gnl\|CDD\|224332	COG1414, IclR, Transcriptional regulator [Transcription].	5.98672e-94

>CP026736.1|AWD67682.1|4440689_4442153_+|hypothetical-protein
MNEPSNFSPYLIKQISDAIDDNIKAKCIDFLYDSHSFFSNEKDFIEDYILNILQDTFVLENKLINPKEIRNPAYKRRQSNKKNWLQTLKKDIKAEKPNPIFEDIELAKKVMDKKVDTEHLKKLFGVEKPEDILKIKLKEVEAWAKSKKTNITDFPYLDSKMNYQIEKAFHTDFTFLIAEIILEKYNGNLSNWIDKRLNSWVEAPIFSNESHSLKTNMEVKTNSQEVVLYNDYKVDEEYFIRTIIPVNDNQTVLSKRNMVLDPKDSQIIQFALSQRDEMFYKEKTVTLDLADIVKEIYNSKGVKNYEAVEARIMKIARYHVEGVAKKKNSRDTKFAINFFQKAVIETDEVTGRRYATIVFSDDTHQGFLNQQFVQIYSDVIKKFKDNSLSSLLLYAFQKERLDCYLQEKSYTHFYPYQYFASKVRFRSKKIDVNLKLINESLAEFVSNQVLIQSYQQKLQGFEISFIPLQNYEIQDYFGGEHRETLEG
>CP026736.1|AWD67681.1|4439494_4440376_-|HAD-family-phosphatase
MLLKPKAIFLDMDGTILNRSNKVSIQTKETIDELRKQGIFVFIATGRAFDEIEELVPEGFQVDGFITSNGMAGYVGKESVFQHSLSLQLVETIIEKARANKVYYELFPYGTPRVTLKQDQPYVENEIKEPKPDSVEINEWLSRKQAIKEEIDWKDSVEGSEFSKFYFFARTHEHIDKWKEELEKLKQETDFTTSTSSKHNVEVMVANVNKATGIQQMLKHFNLPEQDILAIGDSNNDLPMFQFASYAVAMKNAPDHIKEIVDDITEFNCDENGVSHYLSKFLLQKSTRSNPVL
>CP026736.1|AWD67680.1|4438166_4438991_-|kinase/pyrophosphorylase
MNKRKIVYIVSDSVGETAELMVKAVLTQFSGEDIEIQNTSYVENEENIDNIIAEARCNEAIIAYTIVIPPLKQYLDQTAQEEGIVAIDLMTPLMSAFSQTFNKEPNLQPRLTRKLDDNYFRRVEAVEFAVKYDDGQDFRGIKRADLVLIGVSRTSKTPLSMYLAHKSLKVANIPLVPEVTPPDELFTIPKNKCIGLVITPDKLNEIRKERLKNLGLASQANYANVDRILEELDYAEKIMKRIGCPIVNVSGKAVEETAEVILSMLKKDKMKGYV
>CP026736.1|AWD67679.1|4436543_4438046_-|malate:quinone-oxidoreductase
MSNVYKKTDVILIGAGVMSATLGSLLKELAPEWEIKVFEKLADAGEESSNEWNNAGTGHAALCELNYTSEQADGSIDITKAVRINEQFQLSRQFWSYLVSSNLIHHPQEFIMPIPHMSFVQGEKNVSFLKKRFEALSNNLLFQGMEYSDRPEKLKEWMPLVMKGRTSTEAIAATKIDSGTDVNFGALTRMLFTHLKSKSVEINYNHSVENLTRNADGSWEVKVHDIHSGKQEYHTANFVFIGGGGGSLSLLQKTGIPESKHIGGFPVSGLFMVCTNPEVVAQHHGKVYGKAKVGAPPMSVPHLDTRYIDRKKTLLFGPFAGFSPKFLKTGSNLDLINSVKPNNILTMLAAGVKEMGLTKYLIQQVLLSNEKRMEELREFIPNAKSEDWDIVVAGQRVQVIKDTEAGGKGTLQFGTEVVSSADGSVAALLGASPGASTAVHVMLEVLEKCFPQHMKKWEPKIKEMIPSYGLSLVENPALFNEIHVSTAQTLNLSKKELAYR
>CP026736.1|AWD67678.1|4435632_4436292_-|2OG-Fe(II)-oxygenase
MITTNTSLLTKEQTIFSHSGNKIKLEDREINIVARFEEPLVLVLGNVLSTEECDELVQLSKDKMQRSKIGAEREVNSIRTSSGMFFEESENELVHQIEKRLSKIMGPSIEYAEGLQVLKYLPDQEYKAHHDYFTSASKASKNNRISTLVMYLNDVEEGGETYFPKLGLSVSPTKGMAVYFEYFYSDAELNDRTLHGGAPVIKGEKWVATQWMRKQKIRS
>CP026736.1|AWD67677.1|4434407_4435232_-|thermonuclease
MKEIKAIIYIMIAMFMLLLTGCSDEQASHSSTPSTDTKQEVQVEGSSDQEEDHSPSKASREDVVGEAATVERVVDGDTLKVKMLNGKTDRVRLLLVDTPETKHPKLGVQPFGPEASAYTKKRLEGQEITLEFDVQERDQYGRLLAYIWIDNELYNEELLAKGLARVAVFPPNTKYVDEFKKIQEQARKSEKGIWSIENYAQEKGYNTDAVKNQSSSNQKDTQSATSCEGKIKGNQNSKIYHVPTGAHYNQTMNNVIWFCTEKDAQEAGYKKAKN
>CP026736.1|AWD67676.1|4433483_4434038_-|N-acetyltransferase
MKIRKARKEDASAVTELLYDMLGSIARSHTAATTNEEALLRLKAFILSEKNRYSYHHITVAEVDEKVVGLMLCYEGSMLQTLYEPVKRFVRAKLNNPAWEIDIETEHGDYYIDSLCVDEDYRGKGIGTALLNVAKQEAKKRKMFLSLNVEYNNIEAKKLYQRIGFQEHKTIFIDKKPFFYMKTA
>CP026736.1|AWD67675.1|4432725_4433286_-|hypothetical-protein
MNIRDLPFNMSYRFNEKLREVPVNPNQMKQGIIYLKKRALEEEDECAAAKTYGHIGVYERILGELTSSERHLQLAIYLYDRHADTIGEFLNTIRLGHTYHWQEQWDKANETFQLLIEQLKADEDLHIYEDFVYQHYGKCLLDQRVVSRAHHYFQRALEIRLKKGDKELIESTNSCIRLCLNKLKHS
>CP026736.1|AWD67674.1|4431695_4432526_-|YitT-family-protein
MSKIVKDVIFLLIGSFVFSLAVNMFVIPIGLGEGGVTGISIILYYTTHLSTGVTNFILNGILLIVGYKFLEKKTMVYTMITIITTSIFLHVTEGWGLHLHEELIGMVFAGVLLGVGIGMILRVGGTTAGSAIIARILNKFLNWKVSHALLIVDLLVVLASYFIIGIEKLLFTIVMLYITTKVIDFVIEGFDAKKAVTVISSNKNEVADQINEQLDRGVTILNGRGNYTKESKEILYVVINKQELLKLKKLIKKIDDEAFVIVHDVRDVIGKGFLSA
>CP026736.1|AWD67673.1|4430969_4431383_-|DUF4395-domain-containing-protein
MSIPKPLVQLNQLFIVITVLLALLAAKWLLLLPFLVGVVTLVTKRNPVIMAGKKLLKKPISSYQMEDRDQQLFNQWIATICIGIAFISFLLSYTFVGYVFSIMVILAAGVALMGYCIGCTIRYRYMMWKYKRKKHAV
>CP026736.1|AWD67683.1|4443122_4444145_+|hypothetical-protein
MIKKEFAKIGKQIIRQLSSTVEKYKDIEDHMDLDAHGNPTIKTVAEHHRLSKSQISQLIFYHFLHVDERGIICDVSEKEIAAALNCTVRTVRNNNVVLAETELISYSRSGKGINICIVPYPQYFEEHGFGFMELEYTRFEELILIENVNALRLELRKELVYDNDTIKRQFNPGENTSKISFNDYKIFTPKYTHYKGMMQKIAETQASAFKTVVQGSTIFFVLKDGAKNGKMSKQEKRDQYDAAIRRTIEETFVKLSGHSTDSTGIVMSSFQNEDIADLVQLSFEYGIERVKSALYTLIEQAFFSHDAQVVENYGGKIRTLIRKELSKNLQDQVPAELTAS
>CP026736.1|AWD67684.1|4444988_4445585_+|mechanosensitive-ion-channel-protein-MscS
MKYIQWVQQTLFNTGRWIDISEVIIKIILIFIIAQIVIRFARSVINKIFDFRFKTKLLRYNERKEITLKKLCLSIVMYVVYFIALITSLEAIGINMKALLAGISVAGFITGLGAQNLIKDILNGFFILFEDQFSVGDHVSINDTKGTVEEIGLRTTKIRGVDGEFYFFANSNITKVANYTLGEIPEIHTPEQIKTREE
>CP026736.1|AWD67685.1|4445587_4446166_-|HAD-family-hydrolase
MNILWDFDGTIFDTYPTIVKAFKELLTDQTISEDDILKQMKISSDVAIKHFDIEKSLFDKHFHVLEQKINPKDKPAFSHVEDVLSFADTNVIVTHKSRQSTLDILAFFNMKHYFTEIITKDDGYKRKPDSGAYAYLHDKYQLDLVIGDRELDLKPARELGIKTCAFQNNDIEADYHLTSYDQFFTTVVNKNS
>CP026736.1|AWD67686.1|4446293_4447307_-|hypothetical-protein
MIAAIIVGAVILLALIIKGIVKSRRFRLYKEELPDDLGIHKNQGFEDIVHSLEDSLPAAYVQQVKQRMKGKNTKDDEVEWTFFEMKRYFLLNAVIKDVPMFSAKVDEVWHEMIMFTVDYERFTEHFFNRFLHHQPNVETAPSPQPDMRAFFDWMYAHLFDITGNSRLLWGKFFQHPLDKSTLQDFQRLSEDELLDRYFLRNGKWETQQRHLIQTLKEEVTAARTRKKTGGSTYFKKAAKKGNVSNELLLQGFVFYSLYKATNFSSHMNELLLYKKTIYAGDTASYSGYSCSSPWEDNRHHHHDGGHHHHDSGSSCSSSNCSSSSCSSSSCGSGCSSS
>CP026736.1|AWD67687.1|4447379_4447799_-|hypothetical-protein
MSLSAKKILLLVLVVICLVLVVMQFINRSAEPTSGQAQETLSKLPEQETVKEKAKGDEEAHKYRVYYITIEKIENGTYSGTTKEGTGIKFSDKNLNEPIPSNLKKGDIVKAYFDLEYSTKGLVKVEKVDEIPDDDESSS
>CP026736.1|AWD67688.1|4448206_4448698_+|hypothetical-protein
MMFALIVVVGIVALGVASFYVIKRPQGAVDPEDRKPLVSVNGHVKFAEGINQISISKIDMYDQRLVINQVAIIPFNRIQRAEFSKVVKNEKGLHGAPVQRYFGELTIHFTDKNGQETYIRCETPKKNQFHLIYQYDLMKRQLNKRLGLEDQQPYLTLQEPYEL
>CP026736.1|AWD67689.1|4448943_4449780_-|hypothetical-protein
MNELVKIVLNSVDQLFGKNYVQLITYLLIALIGTWLYKEFRTQLLNSENYKREKYSEVLTILLELEYALNRFLINEVEFSQLEENLKAAYPYLSYDLSKEVGSFSTGIEKKKVEEFVERLSAERNNLKYNQPNAFSYLSERSISRSFGHLFRTMLSPVLIPFLLTLLTVVVSFMLLYLTSAMSTVESVVKQYYLIQGVIDYICFLMILVGIADIINNQKVSSMKHLVIFLIIFITLSITAFLLFSKFIIIPTILFVCYGILLKMYLPKIIVGLKINNI
>CP026736.1|AWD67690.1|4449899_4451132_-|aminopeptidase
MEQFERNIEKYAELAVKVGVNIQKGQVLNINADISSADFVREIAKKAYEAGAKSVDVDWHDDHLVRLKYEKADESVFNEFPAWKAQAREELVKNGGAVLSVISSGPDLLKGVDGKRIAAFQKVSGKAMKKFREAIQSDKISWSIVAVPSKDWAAKVFPDVPEERQQEKLWEAIFKSVRVDQEDPVAAWEEHNASLHTKVDILNEKRFSKLHYKAPGTDLTIELPDKHIWVGAGSVNEEGNSFMANMPTEEVFTLPKKDGVNGQVTSTKPLSYAGNLIENFTLTFENGKIVDVKAEEGEETLKHLIETDEGAKYLGEVALVPHQSPISQSNIIFYNTLFDENASNHLAIGSSYSFCLEGGKTMSKEELSQNGANDSITHVDFMIGSAEMDIDGIKADGTNEPVFRKGNWAF
>CP026736.1|AWD67691.1|4451275_4452070_-|IclR-family-transcriptional-regulator
MERENMVKSVSRALDIVTIVSMEREGVGVTEIAKQMDINKSSVYRILTTLAQYGYIEQDQETGRYKLGYKFLEVSSKLLDSIDLRTEAKPFLQELESETNEVIHLVVYDQGEVVYIEKLEGNQTLRMHSKVGKRAPMHCTSVGKAILAFLPLNTVTSILERKGLPAHTDHTITDEESFLRELSEVKQKGYALDLEENEYGITCIAAPIFDHLGQVVAAVSISGATMRMTNERLTYLQERMVDIGKNISERLGYKTESVKDKSLL
>CP026736.1|AWD67692.1|4452122_4452899_-|DUF2437-domain-containing-protein
MKFVRFAVQDSRHTGVLKEDTISVIEGNIFEKWNYTGQTFLVNEVKLLAPLEPNQIIGIGANYAAAKSELPKEMPSIPVFFFKPTSSVIGPNEDIVIPTAIKHVKFESELAVVIGKEAKNIAKEDVLDYIFGYTIGNDVTAPDYFHEDGHWTIGKSFDSFTPLGPVIETELDFKSIHVKAFLNGVEKQNSSTDLMIISVGEMIAYLSTVMTLKPGDVVLTGSPVGAELVSANDIIECTIREIGTLQNPFVAEKERVNV

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Crispr_ID: CP026736_3

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

CP026736_3

4442584-4442670

Orphan

Consensus_repeat	Method
CTTTTGCCGTTTTTGACGTACTACT	CRISPRCasFinder

1 spacers

The CRISPR arrays of CP026736_3

>merge|CP026736|3|4442584-4442670|CRISPRCasFinder
CTTTTGCCATTTTTGACGTACCAAACGATAACTTTTGCTGTTTTTAGCGTACTACCAGGTCGTTTTTGACGTTTTTCACGTACTAAA

>CP026736|3|2|4442584-4442670|CRISPRCasFinder
CTTTTGCCATTTTTGACGTACCAAA	CGATAACTTTTGCTGTTTTTAGCGTACTACCAGGTCG
TTTTTGACGTTTTTCACGTACTAAA

Protein	Signature genes	Signature genes Name	Protein_function
CP026736.1\|AWD67676.1\|4433483_4434038_-\|N-acetyltransferase	unknown	unknown	gnl\|CDD\|366181
CP026736.1\|AWD67690.1\|4449899_4451132_-\|aminopeptidase	unknown	unknown	gnl\|CDD\|376723
CP026736.1\|AWD67677.1\|4434407_4435232_-\|thermonuclease	unknown	unknown	gnl\|CDD\|214615
CP026736.1\|AWD67673.1\|4430969_4431383_-\|DUF4395-domain-containing-protein	unknown	unknown	gnl\|CDD\|379566
CP026736.1\|AWD67679.1\|4436543_4438046_-\|malate:quinone-oxidoreductase	unknown	unknown	gnl\|CDD\|377591
CP026736.1\|AWD67684.1\|4444988_4445585_+\|mechanosensitive-ion-channel-protein-MscS	unknown	unknown	gnl\|CDD\|366370
CP026736.1\|AWD67680.1\|4438166_4438991_-\|kinase/pyrophosphorylase	unknown	unknown	gnl\|CDD\|377080
CP026736.1\|AWD67683.1\|4443122_4444145_+\|hypothetical-protein	unknown	unknown	unknown
CP026736.1\|AWD67686.1\|4446293_4447307_-\|hypothetical-protein	unknown	unknown	gnl\|CDD\|226728
CP026736.1\|AWD67674.1\|4431695_4432526_-\|YitT-family-protein	unknown	unknown	gnl\|CDD\|224203
CP026736.1\|AWD67675.1\|4432725_4433286_-\|hypothetical-protein	unknown	unknown	gnl\|CDD\|276809
CP026736.1\|AWD67678.1\|4435632_4436292_-\|2OG-Fe(II)-oxygenase	unknown	unknown	gnl\|CDD\|177683
CP026736.1\|AWD67681.1\|4439494_4440376_-\|HAD-family-phosphatase	unknown	unknown	gnl\|CDD\|223635
CP026736.1\|AWD67687.1\|4447379_4447799_-\|hypothetical-protein	unknown	unknown	unknown
CP026736.1\|AWD67682.1\|4440689_4442153_+\|hypothetical-protein	unknown	unknown	unknown
CP026736.1\|AWD67685.1\|4445587_4446166_-\|HAD-family-hydrolase	unknown	unknown	gnl\|CDD\|319825
CP026736.1\|AWD67689.1\|4448943_4449780_-\|hypothetical-protein	unknown	unknown	unknown
CP026736.1\|AWD67692.1\|4452122_4452899_-\|DUF2437-domain-containing-protein	unknown	unknown	gnl\|CDD\|223257
CP026736.1\|AWD67688.1\|4448206_4448698_+\|hypothetical-protein	unknown	unknown	unknown
CP026736.1\|AWD67691.1\|4451275_4452070_-\|IclR-family-transcriptional-regulator	unknown	unknown	gnl\|CDD\|224332

Protein	Function_ID	Function_description	E-value
CP026736.1\|AWD67676.1\|4433483_4434038_-\|N-acetyltransferase	gnl\|CDD\|366181	pfam00583, Acetyltransf_1, Acetyltransferase (GNAT) family. This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.	1.1822e-12
CP026736.1\|AWD67690.1\|4449899_4451132_-\|aminopeptidase	gnl\|CDD\|376723	pfam02073, Peptidase_M29, Thermophilic metalloprotease (M29).	0
CP026736.1\|AWD67677.1\|4434407_4435232_-\|thermonuclease	gnl\|CDD\|214615	smart00318, SNc, Staphylococcal nuclease homologues.	4.92218e-39
CP026736.1\|AWD67685.1\|4445587_4446166_-\|HAD-family-hydrolase	gnl\|CDD\|319825	cd07523, HAD_YsbA-like, uncharacterized family of the haloacid dehalogenase-like superfamily, similar to the uncharacterized Lactococcus lactis YsbA. The specific function of Lactococcus lactis YsbA is unknown. Members of this family belong to the haloacid dehalogenase-like (HAD) hydrolases, a large superfamily of diverse enzymes that catalyze carbon or phosphoryl group transfer reactions on a range of substrates, using an active site aspartate in nucleophilic catalysis. Members of this superfamily include 2-L-haloalkanoic acid dehalogenase, azetidine hydrolase, phosphonoacetaldehyde hydrolase, phosphoserine phosphatase, phosphomannomutase, P-type ATPases and many others. HAD hydrolases are found in all three kingdoms of life, and most genomes are predicted to contain multiple HAD-like proteins. Members possess a highly conserved alpha/beta core domain, and many also possess a small cap domain, the fold and function of which is variable. HAD hydrolases are sometimes referred to as belonging to the DDDD superfamily of phosphohydrolases.	1.54713e-42
CP026736.1\|AWD67679.1\|4436543_4438046_-\|malate:quinone-oxidoreductase	gnl\|CDD\|377591	pfam06039, Mqo, Malate:quinone oxidoreductase (Mqo). This family consists of several bacterial Malate:quinone oxidoreductase (Mqo) proteins (EC:1.1.99.16). Mqo takes part in the citric acid cycle. It oxidizes L-malate to oxaloacetate and donates electrons to ubiquinone-1 and other artificial acceptors or, via the electron transfer chain, to oxygen. NAD is not an acceptor and the natural direct acceptor for the enzyme is most likely a quinone. The enzyme is therefore called malate:quinone oxidoreductase, abbreviated to Mqo. Mqo is a peripheral membrane protein and can be released from the membrane by addition of chelators.	0
CP026736.1\|AWD67680.1\|4438166_4438991_-\|kinase/pyrophosphorylase	gnl\|CDD\|377080	pfam03618, Kinase-PPPase, Kinase/pyrophosphorylase. This family of regulatory proteins has ADP-dependent kinase and inorganic phosphate-dependent pyrophosphorylase activity.	3.04958e-145
CP026736.1\|AWD67684.1\|4444988_4445585_+\|mechanosensitive-ion-channel-protein-MscS	gnl\|CDD\|366370	pfam00924, MS_channel, Mechanosensitive ion channel. Two members of this protein family of M. jannaschii have been functionally characterized. Both proteins form mechanosensitive (MS) ion channels upon reconstitution into liposomes and functional examination by the patch-clamp technique. Therefore this family are likely to also be MS channel proteins.	1.0926e-24
CP026736.1\|AWD67674.1\|4431695_4432526_-\|YitT-family-protein	gnl\|CDD\|224203	COG1284, COG1284, Uncharacterized conserved protein [Function unknown].	1.74103e-71
CP026736.1\|AWD67686.1\|4446293_4447307_-\|hypothetical-protein	gnl\|CDD\|226728	COG4278, COG4278, Uncharacterized conserved protein [Function unknown].	2.9051e-08
CP026736.1\|AWD67673.1\|4430969_4431383_-\|DUF4395-domain-containing-protein	gnl\|CDD\|379566	pfam14340, DUF4395, Domain of unknown function (DUF4395). This family of proteins is functionally uncharacterized. This family of proteins is found in bacteria and eukaryotes. Proteins in this family are typically between 142 and 168 amino acids in length. There are two completely conserved C residues that may be functionally important.	7.9414e-22
CP026736.1\|AWD67675.1\|4432725_4433286_-\|hypothetical-protein	gnl\|CDD\|276809	sd00006, TPR, Tetratricopeptide repeat. The Tetratricopeptide repeat (TPR) typically contains 34 amino acids and is found in a variety of organisms including bacteria, cyanobacteria, yeast, fungi, plants, and humans. It is present in a variety of proteins including those involved in chaperone, cell-cycle, transcription, and protein transport complexes. The number of TPR motifs varies among proteins. Those containing 5-6 tandem repeats generate a right-handed helical structure with an amphipathic channel that is thought to accommodate an alpha-helix of a target protein. It has been proposed that TPR proteins preferentially interact with WD-40 repeat proteins, but in many instances several TPR-proteins seem to aggregate to multi-protein complexes.	0.00183094
CP026736.1\|AWD67681.1\|4439494_4440376_-\|HAD-family-phosphatase	gnl\|CDD\|223635	COG0561, Cof, Predicted hydrolases of the HAD superfamily [General function prediction only].	2.29079e-58
CP026736.1\|AWD67678.1\|4435632_4436292_-\|2OG-Fe(II)-oxygenase	gnl\|CDD\|177683	PLN00052, PLN00052, prolyl 4-hydroxylase; Provisional.	9.3072e-41
CP026736.1\|AWD67692.1\|4452122_4452899_-\|DUF2437-domain-containing-protein	gnl\|CDD\|223257	COG0179, MhpD, 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) [Secondary metabolites biosynthesis, transport, and catabolism].	9.01675e-73
CP026736.1\|AWD67691.1\|4451275_4452070_-\|IclR-family-transcriptional-regulator	gnl\|CDD\|224332	COG1414, IclR, Transcriptional regulator [Transcription].	5.98672e-94

>CP026736.1|AWD67682.1|4440689_4442153_+|hypothetical-protein
MNEPSNFSPYLIKQISDAIDDNIKAKCIDFLYDSHSFFSNEKDFIEDYILNILQDTFVLENKLINPKEIRNPAYKRRQSNKKNWLQTLKKDIKAEKPNPIFEDIELAKKVMDKKVDTEHLKKLFGVEKPEDILKIKLKEVEAWAKSKKTNITDFPYLDSKMNYQIEKAFHTDFTFLIAEIILEKYNGNLSNWIDKRLNSWVEAPIFSNESHSLKTNMEVKTNSQEVVLYNDYKVDEEYFIRTIIPVNDNQTVLSKRNMVLDPKDSQIIQFALSQRDEMFYKEKTVTLDLADIVKEIYNSKGVKNYEAVEARIMKIARYHVEGVAKKKNSRDTKFAINFFQKAVIETDEVTGRRYATIVFSDDTHQGFLNQQFVQIYSDVIKKFKDNSLSSLLLYAFQKERLDCYLQEKSYTHFYPYQYFASKVRFRSKKIDVNLKLINESLAEFVSNQVLIQSYQQKLQGFEISFIPLQNYEIQDYFGGEHRETLEG
>CP026736.1|AWD67681.1|4439494_4440376_-|HAD-family-phosphatase
MLLKPKAIFLDMDGTILNRSNKVSIQTKETIDELRKQGIFVFIATGRAFDEIEELVPEGFQVDGFITSNGMAGYVGKESVFQHSLSLQLVETIIEKARANKVYYELFPYGTPRVTLKQDQPYVENEIKEPKPDSVEINEWLSRKQAIKEEIDWKDSVEGSEFSKFYFFARTHEHIDKWKEELEKLKQETDFTTSTSSKHNVEVMVANVNKATGIQQMLKHFNLPEQDILAIGDSNNDLPMFQFASYAVAMKNAPDHIKEIVDDITEFNCDENGVSHYLSKFLLQKSTRSNPVL
>CP026736.1|AWD67680.1|4438166_4438991_-|kinase/pyrophosphorylase
MNKRKIVYIVSDSVGETAELMVKAVLTQFSGEDIEIQNTSYVENEENIDNIIAEARCNEAIIAYTIVIPPLKQYLDQTAQEEGIVAIDLMTPLMSAFSQTFNKEPNLQPRLTRKLDDNYFRRVEAVEFAVKYDDGQDFRGIKRADLVLIGVSRTSKTPLSMYLAHKSLKVANIPLVPEVTPPDELFTIPKNKCIGLVITPDKLNEIRKERLKNLGLASQANYANVDRILEELDYAEKIMKRIGCPIVNVSGKAVEETAEVILSMLKKDKMKGYV
>CP026736.1|AWD67679.1|4436543_4438046_-|malate:quinone-oxidoreductase
MSNVYKKTDVILIGAGVMSATLGSLLKELAPEWEIKVFEKLADAGEESSNEWNNAGTGHAALCELNYTSEQADGSIDITKAVRINEQFQLSRQFWSYLVSSNLIHHPQEFIMPIPHMSFVQGEKNVSFLKKRFEALSNNLLFQGMEYSDRPEKLKEWMPLVMKGRTSTEAIAATKIDSGTDVNFGALTRMLFTHLKSKSVEINYNHSVENLTRNADGSWEVKVHDIHSGKQEYHTANFVFIGGGGGSLSLLQKTGIPESKHIGGFPVSGLFMVCTNPEVVAQHHGKVYGKAKVGAPPMSVPHLDTRYIDRKKTLLFGPFAGFSPKFLKTGSNLDLINSVKPNNILTMLAAGVKEMGLTKYLIQQVLLSNEKRMEELREFIPNAKSEDWDIVVAGQRVQVIKDTEAGGKGTLQFGTEVVSSADGSVAALLGASPGASTAVHVMLEVLEKCFPQHMKKWEPKIKEMIPSYGLSLVENPALFNEIHVSTAQTLNLSKKELAYR
>CP026736.1|AWD67678.1|4435632_4436292_-|2OG-Fe(II)-oxygenase
MITTNTSLLTKEQTIFSHSGNKIKLEDREINIVARFEEPLVLVLGNVLSTEECDELVQLSKDKMQRSKIGAEREVNSIRTSSGMFFEESENELVHQIEKRLSKIMGPSIEYAEGLQVLKYLPDQEYKAHHDYFTSASKASKNNRISTLVMYLNDVEEGGETYFPKLGLSVSPTKGMAVYFEYFYSDAELNDRTLHGGAPVIKGEKWVATQWMRKQKIRS
>CP026736.1|AWD67677.1|4434407_4435232_-|thermonuclease
MKEIKAIIYIMIAMFMLLLTGCSDEQASHSSTPSTDTKQEVQVEGSSDQEEDHSPSKASREDVVGEAATVERVVDGDTLKVKMLNGKTDRVRLLLVDTPETKHPKLGVQPFGPEASAYTKKRLEGQEITLEFDVQERDQYGRLLAYIWIDNELYNEELLAKGLARVAVFPPNTKYVDEFKKIQEQARKSEKGIWSIENYAQEKGYNTDAVKNQSSSNQKDTQSATSCEGKIKGNQNSKIYHVPTGAHYNQTMNNVIWFCTEKDAQEAGYKKAKN
>CP026736.1|AWD67676.1|4433483_4434038_-|N-acetyltransferase
MKIRKARKEDASAVTELLYDMLGSIARSHTAATTNEEALLRLKAFILSEKNRYSYHHITVAEVDEKVVGLMLCYEGSMLQTLYEPVKRFVRAKLNNPAWEIDIETEHGDYYIDSLCVDEDYRGKGIGTALLNVAKQEAKKRKMFLSLNVEYNNIEAKKLYQRIGFQEHKTIFIDKKPFFYMKTA
>CP026736.1|AWD67675.1|4432725_4433286_-|hypothetical-protein
MNIRDLPFNMSYRFNEKLREVPVNPNQMKQGIIYLKKRALEEEDECAAAKTYGHIGVYERILGELTSSERHLQLAIYLYDRHADTIGEFLNTIRLGHTYHWQEQWDKANETFQLLIEQLKADEDLHIYEDFVYQHYGKCLLDQRVVSRAHHYFQRALEIRLKKGDKELIESTNSCIRLCLNKLKHS
>CP026736.1|AWD67674.1|4431695_4432526_-|YitT-family-protein
MSKIVKDVIFLLIGSFVFSLAVNMFVIPIGLGEGGVTGISIILYYTTHLSTGVTNFILNGILLIVGYKFLEKKTMVYTMITIITTSIFLHVTEGWGLHLHEELIGMVFAGVLLGVGIGMILRVGGTTAGSAIIARILNKFLNWKVSHALLIVDLLVVLASYFIIGIEKLLFTIVMLYITTKVIDFVIEGFDAKKAVTVISSNKNEVADQINEQLDRGVTILNGRGNYTKESKEILYVVINKQELLKLKKLIKKIDDEAFVIVHDVRDVIGKGFLSA
>CP026736.1|AWD67673.1|4430969_4431383_-|DUF4395-domain-containing-protein
MSIPKPLVQLNQLFIVITVLLALLAAKWLLLLPFLVGVVTLVTKRNPVIMAGKKLLKKPISSYQMEDRDQQLFNQWIATICIGIAFISFLLSYTFVGYVFSIMVILAAGVALMGYCIGCTIRYRYMMWKYKRKKHAV
>CP026736.1|AWD67683.1|4443122_4444145_+|hypothetical-protein
MIKKEFAKIGKQIIRQLSSTVEKYKDIEDHMDLDAHGNPTIKTVAEHHRLSKSQISQLIFYHFLHVDERGIICDVSEKEIAAALNCTVRTVRNNNVVLAETELISYSRSGKGINICIVPYPQYFEEHGFGFMELEYTRFEELILIENVNALRLELRKELVYDNDTIKRQFNPGENTSKISFNDYKIFTPKYTHYKGMMQKIAETQASAFKTVVQGSTIFFVLKDGAKNGKMSKQEKRDQYDAAIRRTIEETFVKLSGHSTDSTGIVMSSFQNEDIADLVQLSFEYGIERVKSALYTLIEQAFFSHDAQVVENYGGKIRTLIRKELSKNLQDQVPAELTAS
>CP026736.1|AWD67684.1|4444988_4445585_+|mechanosensitive-ion-channel-protein-MscS
MKYIQWVQQTLFNTGRWIDISEVIIKIILIFIIAQIVIRFARSVINKIFDFRFKTKLLRYNERKEITLKKLCLSIVMYVVYFIALITSLEAIGINMKALLAGISVAGFITGLGAQNLIKDILNGFFILFEDQFSVGDHVSINDTKGTVEEIGLRTTKIRGVDGEFYFFANSNITKVANYTLGEIPEIHTPEQIKTREE
>CP026736.1|AWD67685.1|4445587_4446166_-|HAD-family-hydrolase
MNILWDFDGTIFDTYPTIVKAFKELLTDQTISEDDILKQMKISSDVAIKHFDIEKSLFDKHFHVLEQKINPKDKPAFSHVEDVLSFADTNVIVTHKSRQSTLDILAFFNMKHYFTEIITKDDGYKRKPDSGAYAYLHDKYQLDLVIGDRELDLKPARELGIKTCAFQNNDIEADYHLTSYDQFFTTVVNKNS
>CP026736.1|AWD67686.1|4446293_4447307_-|hypothetical-protein
MIAAIIVGAVILLALIIKGIVKSRRFRLYKEELPDDLGIHKNQGFEDIVHSLEDSLPAAYVQQVKQRMKGKNTKDDEVEWTFFEMKRYFLLNAVIKDVPMFSAKVDEVWHEMIMFTVDYERFTEHFFNRFLHHQPNVETAPSPQPDMRAFFDWMYAHLFDITGNSRLLWGKFFQHPLDKSTLQDFQRLSEDELLDRYFLRNGKWETQQRHLIQTLKEEVTAARTRKKTGGSTYFKKAAKKGNVSNELLLQGFVFYSLYKATNFSSHMNELLLYKKTIYAGDTASYSGYSCSSPWEDNRHHHHDGGHHHHDSGSSCSSSNCSSSSCSSSSCGSGCSSS
>CP026736.1|AWD67687.1|4447379_4447799_-|hypothetical-protein
MSLSAKKILLLVLVVICLVLVVMQFINRSAEPTSGQAQETLSKLPEQETVKEKAKGDEEAHKYRVYYITIEKIENGTYSGTTKEGTGIKFSDKNLNEPIPSNLKKGDIVKAYFDLEYSTKGLVKVEKVDEIPDDDESSS
>CP026736.1|AWD67688.1|4448206_4448698_+|hypothetical-protein
MMFALIVVVGIVALGVASFYVIKRPQGAVDPEDRKPLVSVNGHVKFAEGINQISISKIDMYDQRLVINQVAIIPFNRIQRAEFSKVVKNEKGLHGAPVQRYFGELTIHFTDKNGQETYIRCETPKKNQFHLIYQYDLMKRQLNKRLGLEDQQPYLTLQEPYEL
>CP026736.1|AWD67689.1|4448943_4449780_-|hypothetical-protein
MNELVKIVLNSVDQLFGKNYVQLITYLLIALIGTWLYKEFRTQLLNSENYKREKYSEVLTILLELEYALNRFLINEVEFSQLEENLKAAYPYLSYDLSKEVGSFSTGIEKKKVEEFVERLSAERNNLKYNQPNAFSYLSERSISRSFGHLFRTMLSPVLIPFLLTLLTVVVSFMLLYLTSAMSTVESVVKQYYLIQGVIDYICFLMILVGIADIINNQKVSSMKHLVIFLIIFITLSITAFLLFSKFIIIPTILFVCYGILLKMYLPKIIVGLKINNI
>CP026736.1|AWD67690.1|4449899_4451132_-|aminopeptidase
MEQFERNIEKYAELAVKVGVNIQKGQVLNINADISSADFVREIAKKAYEAGAKSVDVDWHDDHLVRLKYEKADESVFNEFPAWKAQAREELVKNGGAVLSVISSGPDLLKGVDGKRIAAFQKVSGKAMKKFREAIQSDKISWSIVAVPSKDWAAKVFPDVPEERQQEKLWEAIFKSVRVDQEDPVAAWEEHNASLHTKVDILNEKRFSKLHYKAPGTDLTIELPDKHIWVGAGSVNEEGNSFMANMPTEEVFTLPKKDGVNGQVTSTKPLSYAGNLIENFTLTFENGKIVDVKAEEGEETLKHLIETDEGAKYLGEVALVPHQSPISQSNIIFYNTLFDENASNHLAIGSSYSFCLEGGKTMSKEELSQNGANDSITHVDFMIGSAEMDIDGIKADGTNEPVFRKGNWAF
>CP026736.1|AWD67691.1|4451275_4452070_-|IclR-family-transcriptional-regulator
MERENMVKSVSRALDIVTIVSMEREGVGVTEIAKQMDINKSSVYRILTTLAQYGYIEQDQETGRYKLGYKFLEVSSKLLDSIDLRTEAKPFLQELESETNEVIHLVVYDQGEVVYIEKLEGNQTLRMHSKVGKRAPMHCTSVGKAILAFLPLNTVTSILERKGLPAHTDHTITDEESFLRELSEVKQKGYALDLEENEYGITCIAAPIFDHLGQVVAAVSISGATMRMTNERLTYLQERMVDIGKNISERLGYKTESVKDKSLL
>CP026736.1|AWD67692.1|4452122_4452899_-|DUF2437-domain-containing-protein
MKFVRFAVQDSRHTGVLKEDTISVIEGNIFEKWNYTGQTFLVNEVKLLAPLEPNQIIGIGANYAAAKSELPKEMPSIPVFFFKPTSSVIGPNEDIVIPTAIKHVKFESELAVVIGKEAKNIAKEDVLDYIFGYTIGNDVTAPDYFHEDGHWTIGKSFDSFTPLGPVIETELDFKSIHVKAFLNGVEKQNSSTDLMIISVGEMIAYLSTVMTLKPGDVVLTGSPVGAELVSANDIIECTIREIGTLQNPFVAEKERVNV

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Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity
CP026736_1	1.1\|2778628\|18\|CP026736\|CRT	2778628-2778645	18	CP026736.1	2981641-2981658	0	1.0
CP026736_1	1.3\|2778712\|18\|CP026736\|CRT	2778712-2778729	18	CP026736.1	2981557-2981574	0	1.0
CP026736_1	1.3\|2778712\|18\|CP026736\|CRT	2778712-2778729	18	CP026736.1	2981599-2981616	0	1.0
CP026736_1	1.1\|2778628\|18\|CP026736\|CRT	2778628-2778645	18	CP026736.1	2981473-2981490	1	0.944
CP026736_1	1.1\|2778628\|18\|CP026736\|CRT	2778628-2778645	18	CP026736.1	2981494-2981511	1	0.944
CP026736_1	1.1\|2778628\|18\|CP026736\|CRT	2778628-2778645	18	CP026736.1	2981515-2981532	1	0.944
CP026736_1	1.1\|2778628\|18\|CP026736\|CRT	2778628-2778645	18	CP026736.1	2981536-2981553	1	0.944
CP026736_1	1.1\|2778628\|18\|CP026736\|CRT	2778628-2778645	18	CP026736.1	2981662-2981679	1	0.944
CP026736_1	1.3\|2778712\|18\|CP026736\|CRT	2778712-2778729	18	CP026736.1	2981473-2981490	1	0.944
CP026736_1	1.3\|2778712\|18\|CP026736\|CRT	2778712-2778729	18	CP026736.1	2981494-2981511	1	0.944
CP026736_1	1.3\|2778712\|18\|CP026736\|CRT	2778712-2778729	18	CP026736.1	2981515-2981532	1	0.944
CP026736_1	1.3\|2778712\|18\|CP026736\|CRT	2778712-2778729	18	CP026736.1	2981536-2981553	1	0.944
CP026736_1	1.3\|2778712\|18\|CP026736\|CRT	2778712-2778729	18	CP026736.1	2981662-2981679	1	0.944

1. spacer 1.1|2778628|18|CP026736|CRT matches to position: 2981641-2981658, mismatch: 0, identity: 1.0

gcgacgagtagaacgacg	CRISPR spacer
gcgacgagtagaacgacg	Protospacer
******************

2. spacer 1.3|2778712|18|CP026736|CRT matches to position: 2981557-2981574, mismatch: 0, identity: 1.0

gcggcgagtagaacggcg	CRISPR spacer
gcggcgagtagaacggcg	Protospacer
******************

3. spacer 1.3|2778712|18|CP026736|CRT matches to position: 2981599-2981616, mismatch: 0, identity: 1.0

gcggcgagtagaacggcg	CRISPR spacer
gcggcgagtagaacggcg	Protospacer
******************

4. spacer 1.1|2778628|18|CP026736|CRT matches to position: 2981473-2981490, mismatch: 1, identity: 0.944

gcgacgagtagaacgacg	CRISPR spacer
gcggcgagtagaacgacg	Protospacer
***.**************

5. spacer 1.1|2778628|18|CP026736|CRT matches to position: 2981494-2981511, mismatch: 1, identity: 0.944

gcgacgagtagaacgacg	CRISPR spacer
gcggcgagtagaacgacg	Protospacer
***.**************

6. spacer 1.1|2778628|18|CP026736|CRT matches to position: 2981515-2981532, mismatch: 1, identity: 0.944

gcgacgagtagaacgacg	CRISPR spacer
gcggcgagtagaacgacg	Protospacer
***.**************

7. spacer 1.1|2778628|18|CP026736|CRT matches to position: 2981536-2981553, mismatch: 1, identity: 0.944

gcgacgagtagaacgacg	CRISPR spacer
gcggcgagtagaacgacg	Protospacer
***.**************

8. spacer 1.1|2778628|18|CP026736|CRT matches to position: 2981662-2981679, mismatch: 1, identity: 0.944

gcgacgagtagaacgacg	CRISPR spacer
gcgacgagtagaacggcg	Protospacer
***************.**

9. spacer 1.3|2778712|18|CP026736|CRT matches to position: 2981473-2981490, mismatch: 1, identity: 0.944

gcggcgagtagaacggcg	CRISPR spacer
gcggcgagtagaacgacg	Protospacer
***************.**

10. spacer 1.3|2778712|18|CP026736|CRT matches to position: 2981494-2981511, mismatch: 1, identity: 0.944

gcggcgagtagaacggcg	CRISPR spacer
gcggcgagtagaacgacg	Protospacer
***************.**

11. spacer 1.3|2778712|18|CP026736|CRT matches to position: 2981515-2981532, mismatch: 1, identity: 0.944

gcggcgagtagaacggcg	CRISPR spacer
gcggcgagtagaacgacg	Protospacer
***************.**

12. spacer 1.3|2778712|18|CP026736|CRT matches to position: 2981536-2981553, mismatch: 1, identity: 0.944

gcggcgagtagaacggcg	CRISPR spacer
gcggcgagtagaacgacg	Protospacer
***************.**

13. spacer 1.3|2778712|18|CP026736|CRT matches to position: 2981662-2981679, mismatch: 1, identity: 0.944

gcggcgagtagaacggcg	CRISPR spacer
gcgacgagtagaacggcg	Protospacer
***.**************

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

667837 : 677144

Bacillus_phage(33.33%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AWD64165.1\|667837_669355_-	ATP-dependent DNA helicase RecQ	L7RCS0	Acanthamoeba_polyphaga_moumouvirus	8.6e-54	32.1
AWD64166.1\|669378_670428_-	recombinase RecQ	NA	NA	NA	NA
AWD64167.1\|670685_670934_+	ferredoxin	A0A127AYY7	Bacillus_phage	3.6e-18	54.1
AWD64168.1\|671015_671591_-	ECF transporter S component	NA	NA	NA	NA
AWD64169.1\|672188_673763_+	phosphoglycerate dehydrogenase	A0A2H4PQU8	Staphylococcus_phage	7.6e-29	30.2
AWD64170.1\|673794_674484_-	LysM peptidoglycan-binding domain-containing protein	A0A2D0ZM66	Rhodococcus_phage	7.2e-16	41.5
AWD64171.1\|674655_676428_-	HAMP domain-containing protein	A0A1V0SGX0	Hokovirus	7.5e-25	26.3
AWD64172.1\|676427_677144_-	DNA-binding response regulator	W8CYM9	Bacillus_phage	2.2e-47	42.2

DBSCAN-SWA_2

1538290 : 1548755

Bacillus_phage(33.33%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AWD68438.1\|1538290_1539523_-	PDZ domain-containing protein	A0A1B1IRD3	uncultured_Mediterranean_phage	8.1e-10	29.4
AWD65010.1\|1539580_1540375_-	MBL fold metallo-hydrolase	A0A2H4J3R0	uncultured_Caudovirales_phage	5.4e-39	35.1
AWD65011.1\|1540393_1541173_-	regulator	NA	NA	NA	NA
AWD65012.1\|1541159_1542527_-	hypothetical protein	NA	NA	NA	NA
AWD65013.1\|1542517_1544425_-	cell wall metabolism sensor histidine kinase WalK	W8CYF6	Bacillus_phage	2.6e-39	36.5
AWD65014.1\|1544431_1545139_-	DNA-binding response regulator	W8CYM9	Bacillus_phage	2.4e-46	42.0
AWD65015.1\|1545916_1547203_-	adenylosuccinate synthase	A0A2P1EKE7	Megavirus	2.1e-69	35.9
AWD65016.1\|1547390_1548755_-	replicative DNA helicase	A0A1P8VVQ6	Streptococcus_phage	1.5e-121	52.1

DBSCAN-SWA_3

1819333 : 1827595

Synechococcus_phage(50.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AWD65249.1\|1819333_1820626_+	adenylosuccinate lyase	A0A2H4UUU6	Bodo_saltans_virus	5.9e-19	25.5
AWD65250.1\|1820700_1821408_+	phosphoribosylaminoimidazolesuccinocarboxamide synthase	A0A0E3FGF0	Synechococcus_phage	2.7e-42	39.3
AWD65251.1\|1821415_1821667_+	phosphoribosylformylglycinamidine synthase subunit PurS	NA	NA	NA	NA
AWD65252.1\|1821663_1822350_+	phosphoribosylformylglycinamidine synthase subunit PurQ	NA	NA	NA	NA
AWD65253.1\|1822333_1824565_+	phosphoribosylformylglycinamidine synthase subunit PurL	A6N228	Microbacterium_phage	2.7e-157	40.8
AWD65254.1\|1824540_1825956_+	amidophosphoribosyltransferase	A0A0M3SGR2	Mollivirus	4.3e-55	34.6
AWD65255.1\|1825982_1827020_+	phosphoribosylformylglycinamidine cyclo-ligase	A0A0E3F0L1	Synechococcus_phage	1.7e-69	45.6
AWD65256.1\|1827016_1827595_+	phosphoribosylglycinamide formyltransferase	M4QRX9	Synechococcus_phage	9.6e-30	39.8

DBSCAN-SWA_4

3777898 : 3786112

Bacillus_virus(83.33%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AWD67039.1\|3777898_3778675_+	NUDIX domain-containing protein	G3MA14	Bacillus_virus	6.6e-34	41.9
AWD67040.1\|3778747_3779296_+	isochorismatase	G3MA16	Bacillus_virus	2.6e-32	44.4
AWD67041.1\|3779319_3780789_+	nicotinate phosphoribosyltransferase	G3MA18	Bacillus_virus	2.5e-114	48.2
AWD67042.1\|3780804_3781485_+	nicotinate (nicotinamide) nucleotide adenylyltransferase	G3MA22	Bacillus_virus	2.2e-41	43.3
AWD67043.1\|3781519_3782341_+	ammonia-dependent NAD(+) synthetase	G3MA24	Bacillus_virus	3.9e-93	64.4
AWD67044.1\|3782522_3782960_+	RNA-binding protein S1	NA	NA	NA	NA
AWD67045.1\|3783700_3786112_+	peptidase S8	A0A217EQY2	Bacillus_phage	1.2e-17	36.4

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

Overview of predicted results

Overview of the results

Cas Category Instructions

Results visualization

1. CP026740

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CRISPR-Cas detection and classification

Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection

2. CP026736

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CRISPR-Cas detection and classification

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Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection