CRISPRimmunity

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Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Self targeting spacer number	Target MGE spacer number	Prophage number	Anti-CRISPR protein number
CP017427	Methylobacterium sp. XJLW plasmid unnamed1, complete sequence	0 crisprs	csa3	0	0	13	0
CP016429	Methylobacterium sp. XJLW chromosome, complete genome	0 crisprs	DEDDh,WYL,csa3,RT,cas3	0	0	5	0

Results visualization

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CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

105251 : 113724

uncultured_Caudovirales_phage(66.67%)

The bacterium proteins that are colored denote the protein is present at specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin' and 'tRNA')

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AWV14155.1\|105251_107693_-	haloacid dehalogenase	A0A218MNH6	uncultured_virus	2.4e-66	34.0
AWV19192.1\|107777_108275_-	DUF305 domain-containing protein	A0A218MND9	uncultured_virus	2.6e-07	33.9
AWV14156.1\|108331_108664_-	ferredoxin	NA	NA	NA	NA
AWV14157.1\|109035_109512_-	hypothetical protein	NA	NA	NA	NA
AWV14158.1\|109606_109819_-	heavy metal transporter	NA	NA	NA	NA
AWV14159.1\|110291_111038_-	arsenical resistance protein ArsH	A0A2H4J5V6	uncultured_Caudovirales_phage	1.2e-80	65.5
AWV14160.1\|111088_111448_+	transcriptional regulator	NA	NA	NA	NA
AWV14161.1\|111440_111968_+	ArsR family transcriptional regulator	A0A2H4J8A6	uncultured_Caudovirales_phage	9.1e-27	44.5
AWV14162.1\|111967_112393_+	arsenate reductase (glutaredoxin)	A0A2H4J8T1	uncultured_Caudovirales_phage	4.9e-47	67.2
AWV14163.1\|112428_113724_+	arsenic transporter	A0A2H4J144	uncultured_Caudovirales_phage	3.7e-138	61.1

DBSCAN-SWA_2

1047155 : 1077384

Stx2-converting_phage(66.67%)

protease,integrase,transposase

attL 1044713:1044729|attR 1066637:1066653

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
1044713:1044729	attL	NA	NA	NA	NA
AWV14901.1\|1047155_1047530_+\|transposase	transposase	NA	NA	NA	NA
AWV14902.1\|1047526_1048507_+\|integrase	integrase	A0A0M3LQ52	Mannheimia_phage	2.9e-10	25.0
AWV14903.1\|1049732_1050098_+\|transposase	IS5 family transposase	NA	NA	NA	NA
AWV14904.1\|1050638_1050848_+	hypothetical protein	NA	NA	NA	NA
AWV14905.1\|1050964_1052245_+	amine oxidase	NA	NA	NA	NA
AWV14906.1\|1052337_1054080_-	hypothetical protein	NA	NA	NA	NA
AWV14907.1\|1054220_1055210_-	alcohol dehydrogenase	NA	NA	NA	NA
AWV14908.1\|1055339_1055933_-	NADPH-dependent F420 reductase	NA	NA	NA	NA
AWV14909.1\|1056982_1058140_-	NADH:flavin oxidoreductase / NADH oxidase	NA	NA	NA	NA
AWV19279.1\|1058310_1058961_+	TetR family transcriptional regulator	NA	NA	NA	NA
AWV19280.1\|1059057_1059885_-\|transposase	transposase	NA	NA	NA	NA
AWV14910.1\|1061521_1063090_-	2-keto-gluconate dehydrogenase	NA	NA	NA	NA
AWV14911.1\|1063205_1063742_-	Twin-arginine translocation pathway signal	NA	NA	NA	NA
AWV14912.1\|1063810_1064212_-	lactoylglutathione lyase	NA	NA	NA	NA
AWV14913.1\|1064547_1064970_+	hypothetical protein	NA	NA	NA	NA
AWV14914.1\|1065537_1065885_+	isocitrate lyase	A0A0P0ZDM8	Stx2-converting_phage	6.2e-32	59.8
AWV14915.1\|1065962_1067519_+\|transposase	IS66 family transposase	A0A0P0ZEB3	Stx2-converting_phage	5.6e-125	48.8
1066637:1066653	attR	NA	NA	NA	NA
AWV14916.1\|1067712_1068918_+	hypothetical protein	NA	NA	NA	NA
AWV14917.1\|1069937_1070822_+	NAD(P)-dependent oxidoreductase	NA	NA	NA	NA
AWV14918.1\|1070991_1071939_-	LysR family transcriptional regulator	NA	NA	NA	NA
AWV14919.1\|1072055_1072664_+	polyisoprenoid-binding protein	NA	NA	NA	NA
AWV14920.1\|1072685_1073294_+	cytochrome B	NA	NA	NA	NA
AWV14921.1\|1073290_1073869_+	polyisoprenoid-binding protein	NA	NA	NA	NA
AWV14922.1\|1073896_1074826_+	glyoxalase	NA	NA	NA	NA
AWV14923.1\|1075313_1075502_+	hypothetical protein	NA	NA	NA	NA
AWV14924.1\|1076814_1077384_+\|protease	protease	NA	NA	NA	NA

DBSCAN-SWA_3

1355327 : 1364408

Paracoccus_phage(42.86%)

tail

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AWV15157.1\|1355327_1355837_-	hypothetical protein	X2CYK8	Brucella_phage	4.4e-10	39.0
AWV19299.1\|1355950_1356484_+	GDSL family lipase	NA	NA	NA	NA
AWV15158.1\|1356523_1357774_-	hypothetical protein	A0A0K1LM54	Rhodobacter_phage	2.0e-40	46.3
AWV15159.1\|1357792_1361701_-	hypothetical protein	A0A0B5A7K5	Paracoccus_phage	4.9e-178	35.6
AWV15160.1\|1361688_1362264_-	peptidase P60	F4YXU4	Roseobacter_phage	1.3e-29	48.6
AWV15161.1\|1362260_1363136_-	beta tubulin	A0A0B5A5B8	Paracoccus_phage	5.3e-56	39.9
AWV15162.1\|1363150_1363792_-	glycoside hydrolase family 24	A0A0B5A2K3	Paracoccus_phage	1.2e-44	48.8
AWV15163.1\|1363835_1364408_-\|tail	phage tail protein	A0A1B2AP03	Escherichia_phage	3.5e-08	40.0

DBSCAN-SWA_4

5144676 : 5153477

Streptococcus_phage(33.33%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AWV18075.1\|5144676_5145729_-	XRE family transcriptional regulator	M1NSC1	Streptococcus_phage	4.6e-06	38.7
AWV19686.1\|5146112_5146826_-	dTMP kinase	M1PSC7	Streptococcus_phage	2.8e-31	42.7
AWV19685.1\|5146845_5148090_-	D-alanyl-D-alanine carboxypeptidase	B6DZZ7	Stx2-converting_phage	1.6e-50	36.2
AWV19687.1\|5148262_5149576_-	hypothetical protein	F5B3X9	Synechococcus_phage	1.6e-19	57.1
AWV18076.1\|5149857_5150196_-	hypothetical protein	NA	NA	NA	NA
AWV18077.1\|5150179_5150827_-	chromosome partitioning protein	W8ECJ6	Mycobacterium_phage	1.2e-12	34.6
AWV19688.1\|5151214_5151760_+	diguanylate cyclase	NA	NA	NA	NA
AWV18078.1\|5151839_5153477_+	acyl-CoA synthetase	A0A2H4PQU7	Staphylococcus_phage	3.7e-34	27.9

DBSCAN-SWA_5

6006857 : 6012210

Mycobacterium_phage(33.33%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AWV18775.1\|6006857_6007406_-	hypothetical protein	A0A249XQ39	Mycobacterium_phage	2.0e-24	39.6
AWV18776.1\|6007872_6008085_-	hypothetical protein	NA	NA	NA	NA
AWV19743.1\|6008146_6008989_-	hypothetical protein	A0A0F6YNR1	Sinorhizobium_phage	2.5e-58	47.7
AWV18777.1\|6008981_6009218_-	hypothetical protein	NA	NA	NA	NA
AWV18778.1\|6009210_6009480_-	hypothetical protein	NA	NA	NA	NA
AWV18779.1\|6009640_6010030_-	hypothetical protein	A0A076G998	Mycobacterium_phage	7.0e-08	33.0
AWV18780.1\|6010178_6010502_-	hypothetical protein	NA	NA	NA	NA
AWV19744.1\|6010498_6010888_-	hypothetical protein	A0A2H4IB20	Erwinia_phage	1.1e-18	43.8
AWV18781.1\|6010900_6011536_-	exonuclease	A0A076G6X1	Sinorhizobium_phage	1.4e-50	50.0
AWV19745.1\|6011538_6012210_-	ERF family protein	K4NWX3	Pseudomonas_phage	8.5e-46	46.9

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

2. CP017427

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CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

0 : 1539

Rhizobium_phage(100.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AWV19793.1\|706_1147_+	hypothetical protein	NA	NA	NA	NA
AWV19794.1\|1179_1539_-	hypothetical protein	B4UTU3	Rhizobium_phage	5.8e-09	39.3

DBSCAN-SWA_2

5815 : 18770

Staphylococcus_phage(14.29%)

integrase,transposase

attL 3004:3018|attR 23614:23628

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
3004:3018	attL	NA	NA	NA	NA
AWV19926.1\|5815_7021_-\|transposase	IS30 family transposase	W5R8L2	Staphylococcus_phage	9.6e-32	31.1
AWV19802.1\|7175_7895_-	TIGR02391 family protein	C7BGE5	Burkholderia_phage	4.2e-35	47.4
AWV19803.1\|7881_8433_-	hypothetical protein	NA	NA	NA	NA
AWV19804.1\|8883_9360_-	hypothetical protein	A0A1B0V7H9	Salmonella_phage	2.0e-49	62.5
AWV19805.1\|9358_9562_+	hypothetical protein	NA	NA	NA	NA
AWV19806.1\|9637_10906_+\|transposase	IS256 family transposase	A0A2P1JQX9	Mycobacterium_phage	2.5e-91	45.7
AWV19807.1\|11542_11884_-	hypothetical protein	NA	NA	NA	NA
AWV19808.1\|11926_13411_-	chemotaxis protein	A0A2H4J162	uncultured_Caudovirales_phage	1.7e-09	48.6
AWV19809.1\|13637_14117_-	hypothetical protein	NA	NA	NA	NA
AWV19810.1\|14342_15185_-	AAA family ATPase	A0A059NT77	Lactococcus_phage	3.3e-39	38.3
AWV19811.1\|17708_18770_+\|integrase	integrase	Q5QBN6	Enterobacteria_phage	7.2e-23	27.7
23614:23628	attR	NA	NA	NA	NA

DBSCAN-SWA_3

33331 : 38496

Diadromus_pulchellus_ascovirus(25.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AWV19818.1\|33331_35542_-	hypothetical protein	F2NZ48	Diadromus_pulchellus_ascovirus	2.2e-26	30.5
AWV19819.1\|36160_36745_-	beta-carotene 15,15'-monooxygenase	A0A076G7E1	Sinorhizobium_phage	1.6e-40	56.8
AWV19820.1\|36741_37485_-	7-cyano-7-deazaguanine synthase QueC	A0A2H4J8Q7	uncultured_Caudovirales_phage	5.9e-48	46.9
AWV19927.1\|37477_37858_-	6-carboxytetrahydropterin synthase QueD	NA	NA	NA	NA
AWV19821.1\|37863_38496_-	7-carboxy-7-deazaguanine synthase	A0A1W6JS84	Salmonella_phage	1.5e-15	31.8

DBSCAN-SWA_4

43824 : 46034

Burkholderia_phage(50.0%)

integrase

attL 41053:41066|attR 48511:48524

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
41053:41066	attL	NA	NA	NA	NA
AWV19826.1\|43824_44400_+\|integrase	integrase	E5FFF9	Burkholderia_phage	2.4e-28	38.3
AWV19827.1\|45602_46034_-	hypothetical protein	G1FTZ6	Mycobacterium_phage	4.8e-10	33.6
48511:48524	attR	NA	NA	NA	NA

DBSCAN-SWA_5

52085 : 53099

Caulobacter_phage(100.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AWV19834.1\|52085_53099_+	antirepressor	A0A1V0EEV1	Caulobacter_phage	1.6e-48	37.3

DBSCAN-SWA_6

62249 : 69762

Erythrobacter_phage(25.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AWV19930.1\|62249_62768_-	hypothetical protein	A0A1P8VVG0	Erythrobacter_phage	1.7e-14	41.2
AWV19840.1\|63044_67403_+	methylase	A0A076FMQ0	Aureococcus_anophage	8.3e-17	22.4
AWV19841.1\|67428_68487_+	DNA primase	A0A0U4B0G9	Pseudomonas_phage	1.4e-10	36.6
AWV19842.1\|68832_69762_+	hypothetical protein	A0A1B0V0Z8	Roseobacter_phage	1.5e-08	30.6

DBSCAN-SWA_7

75418 : 79156

Mycobacterium_phage(100.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AWV19850.1\|75418_79156_+	Ti-type conjugative transfer relaxase TraA	V5UQN3	Mycobacterium_phage	6.7e-15	26.1

DBSCAN-SWA_8

97201 : 97852

Human_gut_gokushovirus(100.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AWV19932.1\|97201_97852_+	hypothetical protein	A0A1X9Q111	Human_gut_gokushovirus	8.3e-06	34.3

DBSCAN-SWA_9

107212 : 110856

Ochrobactrum_phage(66.67%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AWV19879.1\|107212_108439_+	plasmid partitioning protein RepA	A0A240F4U1	Ochrobactrum_phage	1.1e-96	49.7
AWV19933.1\|108435_109440_+	plasmid partitioning protein RepB	A0A240F4U0	Ochrobactrum_phage	9.8e-38	35.7
AWV19880.1\|109644_110856_+	replication initiation protein RepC	L7TKN6	Rhizobium_phage	5.1e-25	30.9

DBSCAN-SWA_10

114063 : 114927

Enterobacteria_phage(100.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AWV19883.1\|114063_114927_-	AAA family ATPase	U5N3V8	Enterobacteria_phage	2.1e-52	48.5

DBSCAN-SWA_11

124717 : 125827

Synechococcus_phage(100.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AWV19891.1\|124717_125827_+	S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase	E3SJ82	Synechococcus_phage	5.0e-35	30.6

DBSCAN-SWA_12

129988 : 155854

Acidianus_filamentous_virus(16.67%)

protease,transposase,bacteriocin

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AWV19896.1\|129988_131965_+	excinuclease ABC subunit B	A7WKN6	Acidianus_filamentous_virus	9.7e-05	32.5
AWV19897.1\|132056_134642_+	excinuclease ABC subunit A	A0A1B1IVR3	uncultured_Mediterranean_phage	2.1e-161	33.6
AWV19935.1\|134987_135872_+	peroxidase	S4VVJ7	Pandoravirus	3.9e-22	31.5
AWV19898.1\|135864_136686_+\|bacteriocin	bacteriocin	NA	NA	NA	NA
AWV19899.1\|136738_137320_+	photosystem reaction center subunit H	NA	NA	NA	NA
AWV19900.1\|137340_137637_+	hypothetical protein	NA	NA	NA	NA
AWV19901.1\|137624_137903_-	ParD protein (antitoxin to ParE)	NA	NA	NA	NA
AWV19902.1\|138019_139939_-	conjugal transfer protein TraG	NA	NA	NA	NA
AWV19903.1\|139960_140971_-	P-type DNA transfer ATPase VirB11	NA	NA	NA	NA
AWV19904.1\|140974_142264_-	type VI secretion protein	NA	NA	NA	NA
AWV19905.1\|142265_143273_-	conjugal transfer protein	NA	NA	NA	NA
AWV19936.1\|143274_143793_-	conjugal transfer protein TraJ	NA	NA	NA	NA
AWV19906.1\|143884_144637_-\|transposase	IS6 family transposase	A0A0N9SKD3	Staphylococcus_phage	1.5e-30	38.0
AWV19907.1\|144679_144943_-	hypothetical protein	NA	NA	NA	NA
AWV19908.1\|145014_145578_-\|protease	protease	NA	NA	NA	NA
AWV19909.1\|146042_147197_-	aldehyde dehydrogenase	NA	NA	NA	NA
AWV19910.1\|147233_147536_-	antibiotic biosynthesis monooxygenase	NA	NA	NA	NA
AWV19911.1\|147770_148097_+\|transposase	transposase	NA	NA	NA	NA
AWV19912.1\|148093_148954_+\|transposase	transposase	A0A0P0I4A4	Acinetobacter_phage	1.1e-53	43.3
AWV19913.1\|149084_149351_-	Hin recombinase	NA	NA	NA	NA
AWV19914.1\|149742_150867_+	alkene reductase	NA	NA	NA	NA
AWV19915.1\|150955_151927_+	LLM class oxidoreductase	NA	NA	NA	NA
AWV19916.1\|152146_152452_+	transcriptional regulator	NA	NA	NA	NA
AWV19917.1\|153025_154231_-	formaldehyde dehydrogenase, glutathione-independent	NA	NA	NA	NA
AWV19937.1\|154375_155035_-	dimethylallyltransferase	NA	NA	NA	NA
AWV19918.1\|155101_155854_+\|transposase	IS6 family transposase	A0A1X9I6C6	Streptococcus_phage	1.0e-31	37.6

DBSCAN-SWA_13

163475 : 166150

Leptospira_phage(50.0%)

transposase

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AWV19923.1\|163475_164312_+\|transposase	transposase	S5WIU1	Leptospira_phage	3.1e-53	43.2
AWV19924.1\|164869_166150_-	sulfate permease	A0A2H4J153	uncultured_Caudovirales_phage	1.1e-30	29.5

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

Overview of predicted results

Overview of the results

Cas Category Instructions

Results visualization

1. CP016429

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection

2. CP017427

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection