CRISPRimmunity

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Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Self targeting spacer number	Target MGE spacer number	Prophage number	Anti-CRISPR protein number
CP021769	Streptococcus agalactiae strain B509 chromosome, complete genome	0 crisprs	cas3,csa3,WYL,DEDDh,csm6,csn2,cas2,cas1,cas9,cas4,cas7,cas8c,cas5	0	0	6	1

Results visualization

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CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

434677 : 446742

Streptomyces_phage(14.29%)

The bacterium proteins that are colored denote the protein is present at specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin' and 'tRNA')

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AWZ27586.1\|434677_435982_-	peptigoglycan-binding protein LysM	A0A2D1GNZ3	Streptomyces_phage	1.7e-05	40.5
AWZ27587.1\|436128_437028_-	zoocin A	A0A221J6U0	Arthrobacter_phage	4.1e-19	45.9
AWZ27588.1\|437220_438768_-	bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/inosine monophosphate cyclohydrolase	Q58MG4	Prochlorococcus_phage	2.5e-77	48.9
AWZ27589.1\|438787_439540_-	GNAT family N-acetyltransferase	NA	NA	NA	NA
AWZ27590.1\|439559_440111_-	phosphoribosylglycinamide formyltransferase	M4QRX9	Synechococcus_phage	1.8e-25	35.6
AWZ27591.1\|440278_441301_-	phosphoribosylformylglycinamidine cyclo-ligase	A0A1D7SE90	Cyanophage	9.6e-65	44.6
AWZ27592.1\|441328_442783_-	amidophosphoribosyltransferase	A0A0M3SGR2	Mollivirus	6.3e-54	32.5
AWZ29098.1\|443016_446742_-	phosphoribosylformylglycinamidine synthase	A6N228	Microbacterium_phage	1.6e-40	27.0

DBSCAN-SWA_2

506762 : 513966

Staphylococcus_phage(33.33%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AWZ27636.1\|506762_508007_+	peptidase M16	A0A1X9I714	Streptococcus_phage	1.7e-87	41.5
AWZ27637.1\|508008_509292_+	peptidase M16	A0A2H4UVM3	Bodo_saltans_virus	1.6e-05	23.6
AWZ27638.1\|509414_509957_+	CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase	NA	NA	NA	NA
AWZ27639.1\|509956_510796_+	energy-coupling factor transporter ATPase	A0A2H4PQG7	Staphylococcus_phage	1.0e-16	26.3
AWZ27640.1\|510771_511614_+	energy-coupling factor transporter ATPase	A0A2H4PQG7	Staphylococcus_phage	2.0e-15	28.5
AWZ27641.1\|511606_512401_+	energy-coupling factor transporter transmembrane protein EcfT	NA	NA	NA	NA
AWZ27642.1\|512616_513156_+	peptidoglycan-binding protein LysM	A0A249XZV3	Enterococcus_phage	6.7e-17	45.8
AWZ27643.1\|513261_513966_+	transglycosylase	A0A2H4J6C5	uncultured_Caudovirales_phage	3.2e-27	56.6

DBSCAN-SWA_3

522286 : 538793

Streptococcus_phage(66.67%)

integrase

attL 524865:524884|attR 539608:539627

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AWZ27651.1\|522286_523642_+	replicative DNA helicase	A0A1P8VVQ6	Streptococcus_phage	1.2e-152	62.6
AWZ27652.1\|523653_523941_+	hypothetical protein	NA	NA	NA	NA
AWZ27653.1\|524270_524882_+	30S ribosomal protein S4	NA	NA	NA	NA
524865:524884	attL	NA	NA	NA	NA
AWZ27654.1\|524988_526161_-\|integrase	site-specific integrase	A0A1X9I5Y5	Streptococcus_phage	1.6e-153	69.6
AWZ27655.1\|526492_527458_-	hypothetical protein	A0A0N9RUA4	Staphylococcus_phage	7.1e-86	51.1
AWZ27656.1\|527975_528185_-	NINE protein	NA	NA	NA	NA
AWZ27657.1\|528284_529055_-	phage repressor protein	A0A1X9I5R1	Streptococcus_phage	7.4e-70	56.3
AWZ27658.1\|529218_529407_+	transcriptional regulator	A0A1X9I5Q5	Streptococcus_phage	5.7e-08	51.7
AWZ27659.1\|529443_530208_+	hypothetical protein	A0A0A7S0G2	Clostridium_phage	1.3e-26	54.8
AWZ27660.1\|530217_530841_+	phage repressor protein	A0A1W6JPC3	Staphylococcus_phage	1.7e-40	42.8
AWZ27661.1\|530840_531110_+	phage antirepressor protein	NA	NA	NA	NA
AWZ27662.1\|531351_531684_+	hypothetical protein	NA	NA	NA	NA
AWZ29101.1\|531683_531875_+	hypothetical protein	NA	NA	NA	NA
AWZ27663.1\|531886_532216_+	hypothetical protein	NA	NA	NA	NA
AWZ27664.1\|532218_532500_+	hypothetical protein	A0A1X9I5U9	Streptococcus_phage	3.2e-23	65.9
AWZ27665.1\|532634_533504_+	hypothetical protein	A0A1X9I6L2	Streptococcus_phage	1.8e-125	75.4
AWZ27666.1\|533523_535026_+	DNA primase	Q9AZI5	Lactococcus_phage	2.0e-63	37.2
AWZ27667.1\|535363_535537_+	hypothetical protein	A0A1X9I5U0	Streptococcus_phage	1.8e-16	78.9
AWZ27668.1\|535557_536088_+	hypothetical protein	A0A1X9I5U4	Streptococcus_phage	2.5e-48	55.9
AWZ27669.1\|536181_536670_+	hypothetical protein	A0A1X9I5V2	Streptococcus_phage	4.4e-44	53.5
AWZ27670.1\|536866_537046_+	hypothetical protein	NA	NA	NA	NA
AWZ27671.1\|537069_537432_+	hypothetical protein	NA	NA	NA	NA
AWZ27672.1\|537406_537793_+	ArpU family transcriptional regulator	A0A141E173	Streptococcus_phage	1.8e-08	32.8
AWZ27673.1\|538286_538793_+	hypothetical protein	D7RWK7	Brochothrix_phage	3.0e-35	48.8
539608:539627	attR	NA	NA	NA	NA

DBSCAN-SWA_4

1017174 : 1029455

Streptococcus_phage(83.33%)

transposase

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AWZ28145.1\|1017174_1017999_+	ABC transporter ATP-binding protein	A0A2H4PQG7	Staphylococcus_phage	4.7e-14	24.3
AWZ28146.1\|1017937_1018648_+	ABC transporter ATP-binding protein	G9BWD6	Planktothrix_phage	4.7e-18	28.5
AWZ28147.1\|1018666_1019326_+	CBS domain-containing protein	M1NSC5	Streptococcus_phage	5.2e-64	55.6
AWZ28148.1\|1019590_1020757_-\|transposase	IS30 family transposase	NA	NA	NA	NA
AWZ28149.1\|1020965_1021601_+	thymidylate kinase	M1PSC7	Streptococcus_phage	2.8e-67	61.1
AWZ28150.1\|1021620_1022484_+	DNA polymerase III subunit delta'	M1NSC1	Streptococcus_phage	7.3e-74	49.7
AWZ28151.1\|1022513_1022840_+	initiation-control protein YabA	M1PFV3	Streptococcus_phage	3.2e-30	61.1
AWZ29116.1\|1022845_1023709_+	rRNA (cytidine-2'-O-)-methyltransferase	M1PLC5	Streptococcus_phage	3.7e-110	70.4
AWZ28152.1\|1023684_1023846_-	hypothetical protein	NA	NA	NA	NA
AWZ28153.1\|1023830_1023935_+	hypothetical protein	NA	NA	NA	NA
AWZ28154.1\|1023978_1024614_-	copper homeostasis protein CutC	NA	NA	NA	NA
AWZ28155.1\|1025906_1026455_+	acetyltransferase	M1PSC3	Streptococcus_phage	1.1e-54	58.4
AWZ28156.1\|1026517_1027699_+	phosphoglycerate dehydrogenase	M1NSB9	Streptococcus_phage	7.0e-168	75.6
AWZ28157.1\|1027753_1028230_+	cysteine methyltransferase	M1PFU9	Streptococcus_phage	7.1e-39	50.9
AWZ28158.1\|1028231_1028588_+	arsenate reductase family protein	M1PLC0	Streptococcus_phage	1.5e-41	71.8
AWZ28159.1\|1028627_1029455_-	exodeoxyribonuclease	M1PSC0	Streptococcus_phage	4.3e-124	78.0

DBSCAN-SWA_5

1248490 : 1316848

Bacillus_phage(40.0%)

protease,integrase,transposase

attL 1290133:1290148|attR 1300191:1300206

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AWZ28362.1\|1248490_1248871_+\|transposase	transposase	NA	NA	NA	NA
AWZ28363.1\|1248870_1249719_+	DegV family protein	NA	NA	NA	NA
AWZ28364.1\|1249903_1251112_+	CCA-adding enzyme	H7BUW3	unidentified_phage	3.3e-40	47.5
AWZ28365.1\|1251122_1252991_+	ABC transporter ATP-binding protein	A0A2K9L3Z8	Tupanvirus	9.6e-63	31.6
AWZ28366.1\|1252987_1253461_+	N-acetyltransferase	NA	NA	NA	NA
AWZ28367.1\|1253527_1255267_+	ABC transporter ATP-binding protein	W8CYL7	Bacillus_phage	1.7e-37	24.5
AWZ28368.1\|1255271_1257041_+	ABC transporter ATP-binding protein	W8CYL7	Bacillus_phage	2.0e-54	29.6
AWZ28369.1\|1257081_1257591_-	hypothetical protein	NA	NA	NA	NA
AWZ28370.1\|1257758_1259108_+	glutamate dehydrogenase	NA	NA	NA	NA
AWZ28371.1\|1259177_1259588_+	peptide deformylase	A0A2I7QLT9	Vibrio_phage	2.7e-10	35.0
AWZ28372.1\|1259624_1261697_-	bifunctional metallophosphatase/5'-nucleotidase	NA	NA	NA	NA
AWZ28373.1\|1261965_1262406_+	MarR family transcriptional regulator	NA	NA	NA	NA
AWZ28374.1\|1262452_1264270_+	ABC transporter ATP-binding protein	W8CYL7	Bacillus_phage	1.1e-34	26.6
AWZ28375.1\|1264259_1266014_+	ABC transporter ATP-binding protein	W8CYL7	Bacillus_phage	6.7e-50	30.2
AWZ28376.1\|1266121_1266748_+	GTP pyrophosphokinase	NA	NA	NA	NA
AWZ28377.1\|1266749_1267436_+	DNA-binding response regulator	NA	NA	NA	NA
AWZ28378.1\|1267435_1268665_+	two-component sensor histidine kinase	NA	NA	NA	NA
AWZ28379.1\|1268780_1269659_+	mevalonate kinase	NA	NA	NA	NA
AWZ28380.1\|1269640_1270585_+	diphosphomevalonate decarboxylase	NA	NA	NA	NA
AWZ28381.1\|1270577_1271570_+	phosphomevalonate kinase	NA	NA	NA	NA
AWZ28382.1\|1272628_1272847_+	DUF4649 domain-containing protein	NA	NA	NA	NA
AWZ28383.1\|1272892_1273747_-	glutathione-dependent reductase	NA	NA	NA	NA
AWZ28384.1\|1273847_1274762_-	diacylglycerol kinase	NA	NA	NA	NA
AWZ29126.1\|1274848_1275493_-	hemolysin III	NA	NA	NA	NA
AWZ28385.1\|1275497_1275947_-	hypothetical protein	NA	NA	NA	NA
AWZ28386.1\|1276038_1277322_-	hydroxymethylglutaryl-CoA reductase, degradative	NA	NA	NA	NA
AWZ29127.1\|1277323_1278496_-	hydroxymethylglutaryl-CoA synthase	NA	NA	NA	NA
AWZ28387.1\|1278649_1279489_+	thymidylate synthase	U5J9N5	Bacillus_phage	9.9e-84	53.6
AWZ28388.1\|1279568_1280063_+	dihydrofolate reductase	A0A0A8JB64	Ralstonia_phage	6.3e-22	35.3
AWZ28389.1\|1280080_1280251_+	hypothetical protein	NA	NA	NA	NA
AWZ29128.1\|1280280_1281507_+\|protease	ATP-dependent Clp protease ATP-binding subunit ClpX	G3M9Z9	Bacillus_virus	8.1e-135	60.0
AWZ28390.1\|1281517_1282114_+	GTP-binding protein	NA	NA	NA	NA
AWZ28391.1\|1282094_1282724_-	TetR family transcriptional regulator	NA	NA	NA	NA
AWZ28392.1\|1282726_1282819_+	hypothetical protein	NA	NA	NA	NA
AWZ28393.1\|1283476_1284127_+	HXXEE domain-containing protein	NA	NA	NA	NA
AWZ28394.1\|1284256_1285633_+	amino acid permease	NA	NA	NA	NA
AWZ28395.1\|1285637_1286582_+	homocysteine S-methyltransferase	NA	NA	NA	NA
AWZ28396.1\|1286675_1288784_-\|protease	ATP-dependent Clp protease ATP-binding subunit	H6X3M6	Enterobacteria_phage	8.2e-119	37.4
AWZ28397.1\|1289217_1289718_+	50S ribosomal protein L10	NA	NA	NA	NA
AWZ28398.1\|1289781_1290147_+	50S ribosomal protein L7/L12	NA	NA	NA	NA
1290133:1290148	attL	NA	NA	NA	NA
AWZ28399.1\|1290387_1293999_+	type II restriction endonuclease	A0A1B1IUC6	uncultured_Mediterranean_phage	1.9e-06	24.3
AWZ28400.1\|1294012_1294504_+	hypothetical protein	NA	NA	NA	NA
AWZ28401.1\|1294555_1295761_+	hypothetical protein	NA	NA	NA	NA
AWZ28402.1\|1295995_1296310_+	hypothetical protein	NA	NA	NA	NA
AWZ28403.1\|1296309_1296813_+	hypothetical protein	NA	NA	NA	NA
AWZ28404.1\|1296876_1297386_+	cell division protein FtsK	NA	NA	NA	NA
AWZ28405.1\|1297387_1297696_+	hypothetical protein	NA	NA	NA	NA
AWZ28406.1\|1297692_1298001_+	hypothetical protein	NA	NA	NA	NA
AWZ28407.1\|1298135_1298702_+	Replication protein RepB	NA	NA	NA	NA
AWZ28408.1\|1298705_1298939_+	hypothetical protein	NA	NA	NA	NA
AWZ28409.1\|1298956_1300138_+\|integrase	site-specific integrase	NA	NA	NA	NA
AWZ29129.1\|1300339_1301557_-	hypothetical protein	NA	NA	NA	NA
1300191:1300206	attR	NA	NA	NA	NA
AWZ28410.1\|1301990_1303805_+	DNA polymerase III subunit epsilon	A0A0A7RWA3	Clostridium_phage	5.0e-32	31.9
AWZ28411.1\|1306819_1310272_+	peptidase C5	A0A218KC60	Bacillus_phage	7.6e-05	39.4
AWZ28412.1\|1310507_1311428_+	adhesion protein	NA	NA	NA	NA
AWZ28413.1\|1311440_1313909_+	histidine triad (HIT) protein	NA	NA	NA	NA
AWZ28414.1\|1314107_1314341_-\|transposase	transposase	NA	NA	NA	NA
AWZ28415.1\|1315742_1316848_-\|transposase	IS3 family transposase	A0A2I7SC85	Paenibacillus_phage	1.8e-69	48.5

DBSCAN-SWA_6

1606760 : 1635922

Streptococcus_phage(82.61%)

transposase

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AWZ28684.1\|1606760_1609865_-	DNA polymerase III subunit alpha	A0A0K1Y8J9	Streptomyces_phage	1.1e-114	28.8
AWZ28685.1\|1610009_1610378_+	GntR family transcriptional regulator	NA	NA	NA	NA
AWZ28686.1\|1610378_1611077_+	ABC transporter ATP-binding protein	A0A2H4PQG7	Staphylococcus_phage	1.9e-19	27.1
AWZ28687.1\|1611088_1611871_+	ABC transporter (permease)	NA	NA	NA	NA
AWZ28688.1\|1612088_1613306_-\|transposase	transposase	A0A1S5SEW7	Streptococcus_phage	3.7e-233	100.0
AWZ28689.1\|1613387_1613591_-	excisionase	A0A1S5SF07	Streptococcus_phage	4.0e-31	100.0
AWZ28690.1\|1613574_1613826_+	hypothetical protein	NA	NA	NA	NA
AWZ28691.1\|1614051_1614282_-	helix-turn-helix domain-containing protein	A0A1S5SEX1	Streptococcus_phage	2.7e-36	100.0
AWZ28692.1\|1614278_1614701_-	sigma-70 family RNA polymerase sigma factor	A0A1S5SEW0	Streptococcus_phage	2.5e-72	100.0
AWZ29137.1\|1614929_1615001_-	hypothetical protein	NA	NA	NA	NA
AWZ28693.1\|1615205_1615559_+	transcriptional regulator	A0A1S5SFA6	Streptococcus_phage	1.3e-58	100.0
AWZ28694.1\|1615618_1615804_-	conjugal transfer protein	D0R0F6	Streptococcus_phage	3.9e-17	67.9
AWZ28695.1\|1615904_1617824_-	tetracycline resistance ribosomal protection protein Tet(M)	A0A1S5SF82	Streptococcus_phage	0.0e+00	100.0
AWZ28696.1\|1617839_1617926_-	tetracycline resistance protein	NA	NA	NA	NA
AWZ28697.1\|1618200_1619133_-	conjugal transfer protein	A0A1S5SF22	Streptococcus_phage	1.1e-171	100.0
AWZ28698.1\|1619129_1620131_-	peptidase P60	A0A1S5SEZ8	Streptococcus_phage	5.0e-191	100.0
AWZ28699.1\|1620127_1622305_-	hypothetical protein	A0A1S5SF30	Streptococcus_phage	0.0e+00	100.0
AWZ28700.1\|1622307_1624755_-	ATP/GTP-binding protein	A0A1S5SF64	Streptococcus_phage	0.0e+00	100.0
AWZ28701.1\|1624738_1625245_-	conjugal transfer protein	A0A1S5SEX7	Streptococcus_phage	8.6e-91	100.0
AWZ28702.1\|1625219_1625717_-	antirestriction protein ArdA	A0A1S5SF25	Streptococcus_phage	1.1e-90	99.4
AWZ28703.1\|1625833_1626055_-	hypothetical protein	A0A1S5SEY0	Streptococcus_phage	4.3e-31	100.0
AWZ28704.1\|1626097_1627303_-	replication initiation protein	A0A1S5SEX3	Streptococcus_phage	2.8e-233	100.0
AWZ28705.1\|1627325_1627478_-	conjugal transfer protein	NA	NA	NA	NA
AWZ28706.1\|1627480_1628866_-	DNA translocase FtsK	A0A1S5SFB5	Streptococcus_phage	1.1e-265	100.0
AWZ28707.1\|1628894_1629281_-	conjugal transfer protein	A0A1S5SF96	Streptococcus_phage	3.0e-64	100.0
AWZ28708.1\|1629296_1629611_-	conjugal transfer protein	A0A1S5SF38	Streptococcus_phage	5.2e-54	100.0
AWZ28709.1\|1629633_1629753_-	hypothetical protein	NA	NA	NA	NA
AWZ28710.1\|1629948_1630557_-	TVP38/TMEM64 family protein	M1Q152	Streptococcus_phage	7.7e-54	55.2
AWZ28711.1\|1631267_1631906_+	chloramphenicol acetyltransferase	A0A2R8FE91	Brazilian_cedratvirus	1.1e-18	42.9
AWZ28712.1\|1632026_1633130_-	hypothetical protein	NA	NA	NA	NA
AWZ28713.1\|1633129_1635922_-	haloacid dehalogenase	M1HK51	Paramecium_bursaria_Chlorella_virus	7.1e-78	29.9

Anti-CRISPR protein detection

Acr_ID: CP021769.1|AWZ27400.1|238974_239301_-|replication-initiation-factor

Click the colored protein region to show detailed information

Acr ID

Acr position

Acr size

Homology with known anti

Neighbor HTH/AcRanker

Neighbor Aca

In prophage

Protospacer in prophage

CP021769.1|AWZ27400.1|238974_239301_-|replication-initiation-factor

238974_239301_-

108 aa aa

hit_Aca_id	identity	query_coverage	hit_coverage
gnl\|BL_ORD_ID\|63	1.0	1	1.0