CRISPRimmunity

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Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Prophage number
CP031100	Clostridium botulinum strain CFSAN034202 plasmid p1_CFSAN034202, complete sequence	0 crisprs	NA	3
CP031099	Clostridium botulinum strain CFSAN034202 plasmid p2_CFSAN034202, complete sequence	0 crisprs	NA	0
CP031098	Clostridium botulinum strain CFSAN034202 chromosome, complete genome	0 crisprs	RT,DEDDh,cas3,WYL,csa3,DinG	5

Results visualization

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CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

0 : 28242

uncultured_Caudovirales_phage(18.75%)

tail,head,terminase,capsid,portal

The bacterium proteins that are colored denote the protein is present at specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin' and 'tRNA')

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AXG97740.1\|2675_3038_-	hypothetical protein	NA	NA	NA	NA
AXG97741.1\|3061_3271_-	hypothetical protein	NA	NA	NA	NA
AXG97742.1\|3286_3703_-	hypothetical protein	NA	NA	NA	NA
AXG97743.1\|3717_4749_-	DUF3383 family protein	A0A2D1GQ64	Lysinibacillus_phage	4.7e-19	31.9
AXG97744.1\|4753_5281_-	hypothetical protein	NA	NA	NA	NA
AXG97745.1\|5273_5615_-	hypothetical protein	NA	NA	NA	NA
AXG97746.1\|5634_6144_-	hypothetical protein	E5DV55	Deep-sea_thermophilic_phage	3.3e-26	40.9
AXG97747.1\|6140_6746_-\|head,tail	phage gp6-like head-tail connector protein	NA	NA	NA	NA
AXG97748.1\|6792_6990_-	hypothetical protein	NA	NA	NA	NA
AXG97749.1\|6998_7325_-	hypothetical protein	NA	NA	NA	NA
AXG97750.1\|7338_8436_-\|capsid	phage major capsid protein	B0VK33	Azospirillum_phage	2.4e-66	35.4
AXG97751.1\|8435_9365_-	hypothetical protein	A0A2D1GNL2	Pseudomonas_phage	4.1e-14	37.6
AXG97752.1\|9510_9756_-	hypothetical protein	NA	NA	NA	NA
AXG97753.1\|9971_10361_-	single-stranded DNA-binding protein	A0A0K0MWE1	Streptococcus_phage	1.3e-27	48.9
AXG97754.1\|10903_11122_-	hypothetical protein	NA	NA	NA	NA
AXG97755.1\|11124_12093_-	hypothetical protein	NA	NA	NA	NA
AXG97822.1\|12092_12356_-	DUF1599 domain-containing protein	A0A2H4J4N1	uncultured_Caudovirales_phage	1.0e-31	79.1
AXG97756.1\|12658_12865_-	hypothetical protein	NA	NA	NA	NA
AXG97757.1\|13193_13454_-	hypothetical protein	NA	NA	NA	NA
AXG97758.1\|13484_13835_-	hypothetical protein	NA	NA	NA	NA
AXG97759.1\|13866_14049_-	hypothetical protein	NA	NA	NA	NA
AXG97823.1\|14090_14750_-	DNA modification methylase	A0A1S5S9L2	Streptococcus_phage	8.0e-89	67.6
AXG97760.1\|14791_15220_-	phage N-6-adenine-methyltransferase	A0A0A7RUD1	Clostridium_phage	3.5e-69	87.1
AXG97824.1\|15266_15506_-	hypothetical protein	NA	NA	NA	NA
AXG97761.1\|15666_16434_-\|head	phage head morphogenesis protein	W8VT12	Pseudomonas_phage	1.4e-07	33.9
AXG97762.1\|16475_17789_-\|portal	phage portal protein	A0A0F7L7L5	uncultured_marine_virus	1.1e-23	23.0
AXG97763.1\|18075_18474_-	hypothetical protein	NA	NA	NA	NA
AXG97764.1\|18707_18962_-	hypothetical protein	NA	NA	NA	NA
AXG97765.1\|19057_19441_-	hypothetical protein	NA	NA	NA	NA
AXG97766.1\|19455_19698_-	hypothetical protein	NA	NA	NA	NA
AXG97767.1\|19825_20530_-	helix-turn-helix domain-containing protein	F0PIG9	Enterococcus_phage	9.3e-19	46.8
AXG97768.1\|20549_20813_-	hypothetical protein	NA	NA	NA	NA
AXG97769.1\|21301_21493_-	DUF4236 domain-containing protein	A0A2K9V2W7	Faecalibacterium_phage	4.1e-14	58.7
AXG97770.1\|21694_22111_-	hypothetical protein	NA	NA	NA	NA
AXG97771.1\|23234_24479_-\|terminase	PBSX family phage terminase large subunit	A0A0S0KEN9	Bacillus_phage	7.6e-149	61.7
AXG97772.1\|24481_24892_-\|terminase	terminase small subunit	A0A0A8WDX8	Clostridium_phage	4.0e-22	42.8
AXG97773.1\|24881_25187_-	hypothetical protein	NA	NA	NA	NA
AXG97774.1\|25256_27608_-	hypothetical protein	A0A2H4J056	uncultured_Caudovirales_phage	1.1e-156	40.5
AXG97775.1\|27592_27826_-	hypothetical protein	NA	NA	NA	NA
AXG97776.1\|27837_28242_-	hypothetical protein	A0A2H4J7M8	uncultured_Caudovirales_phage	3.3e-13	37.0

DBSCAN-SWA_2

32794 : 49679

Clostridium_phage(57.14%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AXG97784.1\|32794_32977_-	hypothetical protein	A0A0A7RTH9	Clostridium_phage	6.5e-17	82.5
AXG97785.1\|33500_34130_-	FAD-dependent thymidylate synthase	F8UBK4	Clostridium_phage	2.0e-44	47.1
AXG97786.1\|34424_34892_-	hypothetical protein	D9ZNH9	Clostridium_phage	9.2e-15	39.5
AXG97787.1\|34948_35839_-	helix-turn-helix domain-containing protein	NA	NA	NA	NA
AXG97788.1\|35859_36369_-	dUTPase	S6AVW3	Thermus_phage	8.2e-25	39.9
AXG97789.1\|36369_36579_-	hypothetical protein	NA	NA	NA	NA
AXG97790.1\|36581_37136_-	hypothetical protein	Q332D2	Clostridium_botulinum_C_phage	4.3e-43	50.5
AXG97791.1\|37150_37384_-	hypothetical protein	NA	NA	NA	NA
AXG97792.1\|37567_37834_-	hypothetical protein	NA	NA	NA	NA
AXG97793.1\|37827_39684_-	bifunctional DNA primase/helicase	K4NWL6	Pseudomonas_phage	6.3e-14	24.2
AXG97794.1\|39696_40452_-	hypothetical protein	NA	NA	NA	NA
AXG97795.1\|40475_40721_-	stage III sporulation protein D	M9Q261	Clostridium_phage	6.3e-15	52.0
AXG97796.1\|40720_41038_-	hypothetical protein	NA	NA	NA	NA
AXG97797.1\|41336_41948_-	hypothetical protein	A0A0A7RVW0	Clostridium_phage	2.7e-62	62.3
AXG97798.1\|41963_42851_-	hypothetical protein	A0A088FAU5	Sulfitobacter_phage	1.9e-69	47.3
AXG97799.1\|42869_43355_-	hypothetical protein	A0A059T5F1	Listeria_phage	1.2e-20	39.6
AXG97800.1\|43822_44206_+	XRE family transcriptional regulator	NA	NA	NA	NA
AXG97801.1\|44480_45530_-	hypothetical protein	A0A2H4JAA4	uncultured_Caudovirales_phage	3.3e-20	43.2
AXG97802.1\|45933_46500_+	hypothetical protein	NA	NA	NA	NA
AXG97803.1\|46617_47529_+	ATPase	NA	NA	NA	NA
AXG97804.1\|47512_47800_+	hypothetical protein	NA	NA	NA	NA
AXG97805.1\|47877_48294_-	hypothetical protein	I2E8W8	Clostridium_phage	8.2e-07	27.7
AXG97806.1\|48304_49096_-	N-acetylmuramoyl-L-alanine amidase	I1TJX3	Clostridium_phage	5.5e-68	52.5
AXG97807.1\|49097_49427_-	hypothetical protein	NA	NA	NA	NA
AXG97808.1\|49526_49679_-	XkdX family protein	A0A249XXC6	Clostridium_phage	2.4e-09	62.2

DBSCAN-SWA_3

52974 : 56186

Aeribacillus_phage(66.67%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AXG97814.1\|52974_54141_-	hypothetical protein	G0YPL8	Erwinia_phage	1.9e-21	36.9
AXG97815.1\|54133_54505_-	hypothetical protein	NA	NA	NA	NA
AXG97816.1\|54509_55046_-	hypothetical protein	NA	NA	NA	NA
AXG97817.1\|55045_55858_-	hypothetical protein	A0A1L2JY62	Aeribacillus_phage	1.4e-31	30.6
AXG97818.1\|55850_56186_-	hypothetical protein	A0A1L2JY63	Aeribacillus_phage	3.5e-08	34.5

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

2. CP031098

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CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

1028624 : 1036828

uncultured_Caudovirales_phage(33.33%)

protease

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AXG95051.1\|1028624_1029581_-	chemotaxis protein	A0A2H4J162	uncultured_Caudovirales_phage	7.2e-14	38.6
AXG95052.1\|1029727_1030546_-\|protease	CPBP family intramembrane metalloprotease	NA	NA	NA	NA
AXG95053.1\|1030847_1031498_-	7-cyano-7-deazaguanine synthase QueC	A0A2H4J8Q7	uncultured_Caudovirales_phage	1.7e-59	51.2
AXG95054.1\|1031681_1032272_-	GTP cyclohydrolase I FolE	S4U0J3	uncultured_phage	9.8e-46	54.5
AXG95055.1\|1032275_1032941_-	putative 7-carboxy-7-deazaguanine synthase QueE	S4TZT1	uncultured_phage	1.4e-37	44.6
AXG95056.1\|1032942_1033374_-	6-carboxytetrahydropterin synthase QueD	A0A1U9WRB3	Streptococcus_virus	1.4e-12	33.1
AXG95057.1\|1033391_1034153_-	2-hydroxyglutaryl-CoA dehydratase	NA	NA	NA	NA
AXG95058.1\|1034269_1035265_-	2-hydroxyacyl-CoA dehydratase	NA	NA	NA	NA
AXG95059.1\|1035883_1036828_+	YafY family transcriptional regulator	A0A1B0RXM1	Streptococcus_phage	3.2e-14	31.1

DBSCAN-SWA_2

1344434 : 1384444

Clostridium_phage(52.5%)

portal,tail,integrase,plate,coat,terminase,head

attL 1370043:1370060|attR 1388678:1388695

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AXG95332.1\|1344434_1345202_-	N-acetylmuramoyl-L-alanine amidase	A0A2H4J8A3	uncultured_Caudovirales_phage	3.5e-88	65.1
AXG95333.1\|1345241_1345436_-	hypothetical protein	NA	NA	NA	NA
AXG95334.1\|1345452_1345707_-	hypothetical protein	A0A0A7RTX0	Clostridium_phage	9.1e-25	68.4
AXG95335.1\|1345802_1346147_-	hypothetical protein	A0A2H4JGH3	uncultured_Caudovirales_phage	6.4e-05	60.0
AXG95336.1\|1346414_1346819_-	hypothetical protein	NA	NA	NA	NA
AXG95337.1\|1347228_1347573_-	hypothetical protein	A0A2H4J342	uncultured_Caudovirales_phage	1.8e-31	62.6
AXG95338.1\|1347583_1348765_-\|tail	phage tail protein	A0A2H4J039	uncultured_Caudovirales_phage	4.6e-71	62.3
AXG95339.1\|1348768_1349395_-	DUF2313 domain-containing protein	A0A0A7RVP9	Clostridium_phage	4.6e-86	74.1
AXG95340.1\|1349375_1350470_-\|plate	baseplate J/gp47 family protein	A0A0A7S096	Clostridium_phage	4.0e-162	77.5
AXG95341.1\|1350473_1350881_-	DUF2634 domain-containing protein	A0A0A7RTH1	Clostridium_phage	3.6e-55	84.6
AXG95342.1\|1350883_1351228_-	DUF2577 domain-containing protein	A0A0A7RTJ2	Clostridium_phage	6.3e-37	73.7
AXG95343.1\|1351230_1352205_-	hypothetical protein	A0A0A7RTZ4	Clostridium_phage	1.6e-157	84.9
AXG95344.1\|1352216_1352885_-	LysM peptidoglycan-binding domain-containing protein	A0A0A7RVP5	Clostridium_phage	2.0e-95	75.0
AXG95345.1\|1352884_1354909_-	hypothetical protein	A0A0A7S091	Clostridium_phage	6.8e-155	51.4
AXG95346.1\|1354949_1355627_-	hypothetical protein	NA	NA	NA	NA
AXG95347.1\|1355884_1356298_-	hypothetical protein	A0A0A7RTN3	Clostridium_phage	1.1e-51	74.8
AXG95348.1\|1356313_1356778_-\|portal	phage portal protein	A0A0A7RVP1	Clostridium_phage	1.9e-73	87.7
AXG95349.1\|1356781_1358092_-\|tail	phage tail sheath protein	A0A0A7S087	Clostridium_phage	5.5e-214	85.8
AXG95350.1\|1358253_1358712_-	hypothetical protein	A0A0A7RTI2	Clostridium_phage	3.8e-53	76.2
AXG95351.1\|1358698_1359190_-	HK97 gp10 family phage protein	A0A0A7RTT0	Clostridium_phage	2.4e-61	74.1
AXG95352.1\|1359189_1359576_-	hypothetical protein	A0A0A7S083	Clostridium_phage	7.8e-44	75.2
AXG97682.1\|1359577_1359922_-	hypothetical protein	A0A0A7RTX9	Clostridium_phage	6.3e-45	77.2
AXG95353.1\|1359941_1361009_-\|coat	phage coat protein	A0A0A7RTH8	Clostridium_phage	1.7e-181	88.7
AXG95354.1\|1361031_1361601_-	scaffolding protein	A0A0A7RTM5	Clostridium_phage	1.8e-33	52.1
AXG95355.1\|1361619_1361979_-	ABC transporter ATPase	A0A2H4J4N9	uncultured_Caudovirales_phage	1.8e-50	82.4
AXG95356.1\|1362093_1362366_-	hypothetical protein	NA	NA	NA	NA
AXG95357.1\|1362432_1363455_-\|head	phage head morphogenesis protein	A0A0A7RVY7	Clostridium_phage	1.0e-151	78.2
AXG95358.1\|1363444_1364887_-\|portal	phage portal protein	A0A0A7S074	Clostridium_phage	4.7e-251	89.1
AXG95359.1\|1364899_1366309_-\|terminase	terminase	A0A0A7RTS1	Clostridium_phage	3.2e-212	87.9
AXG95360.1\|1366301_1366877_-\|terminase	terminase small subunit	Q6SED9	Lactobacillus_prophage	2.9e-10	31.6
AXG95361.1\|1366975_1367167_-	hypothetical protein	NA	NA	NA	NA
AXG95362.1\|1367352_1367790_-	siderophore-interacting protein	A0A2H4J015	uncultured_Caudovirales_phage	5.2e-20	40.4
AXG95363.1\|1368154_1368334_-	hypothetical protein	NA	NA	NA	NA
AXG95364.1\|1368363_1370229_-	DNA primase	A0A2H4J1M0	uncultured_Caudovirales_phage	0.0e+00	88.2
1370043:1370060	attL	NA	NA	NA	NA
AXG95365.1\|1370255_1371245_-	DNA polymerase III subunit beta	NA	NA	NA	NA
AXG95366.1\|1371241_1371565_-	hypothetical protein	NA	NA	NA	NA
AXG95367.1\|1371567_1371897_-	nucleotide pyrophosphohydrolase	A0A2H4J7H8	uncultured_Caudovirales_phage	5.1e-44	78.0
AXG95368.1\|1371897_1372167_-	DUF1599 domain-containing protein	A0A2H4J4N1	uncultured_Caudovirales_phage	3.3e-33	82.8
AXG95369.1\|1372352_1372568_-	hypothetical protein	NA	NA	NA	NA
AXG95370.1\|1372596_1372914_-	hypothetical protein	NA	NA	NA	NA
AXG95371.1\|1372958_1374653_-	hypothetical protein	A0A2H4J041	uncultured_Caudovirales_phage	3.8e-292	84.6
AXG95372.1\|1374869_1375328_-	DUF669 domain-containing protein	A0A2H4J1S8	uncultured_Caudovirales_phage	3.3e-57	66.7
AXG95373.1\|1375330_1375651_-	hypothetical protein	NA	NA	NA	NA
AXG95374.1\|1375650_1377333_-	NTP-binding protein	A0A2H4J7Q2	uncultured_Caudovirales_phage	1.3e-215	74.1
AXG95375.1\|1377378_1377675_-	VRR-NUC domain-containing protein	A0A2H4J095	uncultured_Caudovirales_phage	1.1e-40	80.2
AXG95376.1\|1377747_1378572_-	DUF1351 domain-containing protein	A0A2H4J082	uncultured_Caudovirales_phage	2.9e-96	67.9
AXG95377.1\|1378572_1379673_-	DEAD/DEAH box helicase	A0A2H4J064	uncultured_Caudovirales_phage	2.4e-170	78.5
AXG95378.1\|1380017_1380311_-	histidine kinase	NA	NA	NA	NA
AXG95379.1\|1380330_1381080_-	hypothetical protein	Q332L4	Clostridium_botulinum_C_phage	1.0e-15	36.6
AXG95380.1\|1381096_1381426_-	hypothetical protein	NA	NA	NA	NA
AXG95381.1\|1381425_1381629_-	XRE family transcriptional regulator	NA	NA	NA	NA
AXG95382.1\|1381837_1382350_+	helix-turn-helix domain-containing protein	A0A0A7RTK4	Clostridium_phage	6.1e-12	29.5
AXG95383.1\|1382397_1382814_+	ImmA/IrrE family metallo-endopeptidase	I3VYZ2	Thermoanaerobacterium_phage	2.4e-06	30.1
AXG95384.1\|1383373_1384444_+\|integrase	site-specific integrase	B3GVW7	Streptococcus_phage	1.5e-31	33.1
1388678:1388695	attR	NA	NA	NA	NA

DBSCAN-SWA_3

3515090 : 3547393

Clostridium_phage(74.07%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AXG97245.1\|3515090_3516578_-	MBOAT family protein	A0A125RNP0	Pseudomonas_phage	4.3e-66	38.3
AXG97246.1\|3517672_3517822_-	hypothetical protein	NA	NA	NA	NA
AXG97247.1\|3518123_3518534_-	helix-turn-helix domain-containing protein	A0A0A7RUJ5	Clostridium_phage	1.0e-09	56.2
AXG97248.1\|3518725_3518935_+	XRE family transcriptional regulator	NA	NA	NA	NA
AXG97249.1\|3518975_3519209_+	XRE family transcriptional regulator	NA	NA	NA	NA
AXG97250.1\|3519293_3520208_+	hypothetical protein	A8ASN4	Listeria_phage	4.3e-40	40.2
AXG97251.1\|3520149_3520998_+	AAA family ATPase	A0A2K9V3L7	Faecalibacterium_phage	3.6e-33	35.2
AXG97252.1\|3521054_3521375_+	hypothetical protein	NA	NA	NA	NA
AXG97253.1\|3521488_3522010_+	hypothetical protein	A0A0A7RVS1	Clostridium_phage	3.6e-36	50.6
AXG97254.1\|3522676_3522961_+	hypothetical protein	A0A0A7S0R4	Clostridium_phage	3.0e-24	59.2
AXG97255.1\|3522960_3523326_+	hypothetical protein	A0A0A7RW73	Clostridium_phage	1.2e-33	57.9
AXG97256.1\|3523330_3523678_+	hypothetical protein	A0A0A7RU51	Clostridium_phage	7.8e-35	58.9
AXG97257.1\|3523683_3524103_+	hypothetical protein	A0A0A7RU17	Clostridium_phage	1.1e-56	71.9
AXG97258.1\|3524107_3524995_+	hypothetical protein	A0A0A7RTZ9	Clostridium_phage	1.2e-119	75.0
AXG97259.1\|3525009_3525426_+	esterase	A0A0A7S0S0	Clostridium_phage	9.0e-46	71.2
AXG97724.1\|3525460_3525628_+	hypothetical protein	A0A0A7RW80	Clostridium_phage	1.9e-15	70.4
AXG97260.1\|3525791_3527195_+	hypothetical protein	A0A0A7RU22	Clostridium_phage	5.2e-05	42.4
AXG97261.1\|3527207_3527642_+	hypothetical protein	NA	NA	NA	NA
AXG97262.1\|3527628_3528714_+	hypothetical protein	NA	NA	NA	NA
AXG97263.1\|3528726_3529068_+	hypothetical protein	A0A0A7S0S4	Clostridium_phage	2.1e-16	55.7
AXG97264.1\|3529082_3530432_+	caspase family protein	A0A0A7RW86	Clostridium_phage	2.1e-75	74.6
AXG97265.1\|3530433_3531411_+	hypothetical protein	A0A0A7RU61	Clostridium_phage	9.3e-110	60.2
AXG97266.1\|3531433_3532942_+	hypothetical protein	A0A0A7RU28	Clostridium_phage	5.5e-93	37.7
AXG97267.1\|3532953_3534975_+	hypothetical protein	A0A0A7RU09	Clostridium_phage	1.1e-56	48.5
AXG97268.1\|3534977_3535253_+	hypothetical protein	A0A223LE82	Bacillus_phage	3.9e-05	38.8
AXG97269.1\|3535264_3536263_+	hypothetical protein	A0A0A7RW91	Clostridium_phage	2.1e-141	72.6
AXG97270.1\|3536274_3537360_+	signal peptidase II	A0A0A7RU66	Clostridium_phage	4.2e-143	70.4
AXG97271.1\|3537392_3538310_+	hypothetical protein	NA	NA	NA	NA
AXG97272.1\|3538312_3539092_+	hypothetical protein	NA	NA	NA	NA
AXG97273.1\|3539143_3539911_+	hypothetical protein	NA	NA	NA	NA
AXG97274.1\|3539976_3540801_+	carbohydrate-binding protein	A0A0A7RU33	Clostridium_phage	7.3e-15	30.4
AXG97275.1\|3540866_3541676_+	cell adhesion protein	A0A0A7RU33	Clostridium_phage	2.8e-27	34.7
AXG97276.1\|3541831_3542086_+	hypothetical protein	A0A0A7RTX0	Clostridium_phage	4.8e-26	70.9
AXG97277.1\|3542104_3542299_+	hypothetical protein	NA	NA	NA	NA
AXG97278.1\|3542445_3543225_+	N-acetylmuramoyl-L-alanine amidase	A0A2H4J8A3	uncultured_Caudovirales_phage	1.2e-88	63.4
AXG97279.1\|3543623_3544748_+	chorismate synthase	A0A291AU41	Pandoravirus	3.8e-30	28.5
AXG97280.1\|3544765_3547393_+	DNA polymerase I	F8WQ35	Bacillus_phage	4.2e-64	35.4

DBSCAN-SWA_4

3563422 : 3651374

Clostridium_phage(75.51%)

portal,tRNA,tail,integrase,plate,protease,coat,transposase,terminase,head

attL 3583377:3583436|attR 3627822:3627882

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AXG97300.1\|3563422_3564274_+\|protease	metalloprotease	NA	NA	NA	NA
AXG97301.1\|3564386_3566237_+	TIGR03960 family B12-binding radical SAM protein	NA	NA	NA	NA
AXG97302.1\|3566214_3566928_+	DUF2344 domain-containing protein	NA	NA	NA	NA
AXG97303.1\|3566966_3568406_+	ribonuclease E/G	NA	NA	NA	NA
AXG97304.1\|3568529_3568844_+	50S ribosomal protein L21	NA	NA	NA	NA
AXG97305.1\|3568846_3569173_+\|protease	ribosomal-processing cysteine protease Prp	NA	NA	NA	NA
AXG97306.1\|3569175_3569478_+	50S ribosomal protein L27	NA	NA	NA	NA
AXG97307.1\|3569856_3571131_+	GTPase Obg	NA	NA	NA	NA
AXG97308.1\|3571157_3571451_+	ribosome assembly RNA-binding protein YhbY	NA	NA	NA	NA
AXG97309.1\|3571693_3572299_+	nicotinate-nucleotide adenylyltransferase	NA	NA	NA	NA
AXG97310.1\|3572381_3572951_+	HD domain-containing protein	NA	NA	NA	NA
AXG97311.1\|3572961_3574236_+	LytR family transcriptional regulator	A0A1X9I5X1	Streptococcus_phage	1.7e-18	29.2
AXG97312.1\|3574251_3574632_+	RidA family protein	NA	NA	NA	NA
AXG97313.1\|3574957_3575860_+	RluA family pseudouridine synthase	NA	NA	NA	NA
AXG97314.1\|3575967_3577215_-	D-alanyl-D-alanine carboxypeptidase	NA	NA	NA	NA
AXG97315.1\|3577403_3578012_+	DNA-binding protein	NA	NA	NA	NA
AXG97316.1\|3578117_3579197_+	selenide, water dikinase SelD	NA	NA	NA	NA
AXG97317.1\|3579263_3580652_+\|tRNA	L-seryl-tRNA(Sec) selenium transferase	NA	NA	NA	NA
AXG97318.1\|3580670_3582578_+	selenocysteine-specific translation elongation factor	A0A2K9KZ60	Tupanvirus	2.8e-17	30.9
3583377:3583436	attL	NA	NA	NA	NA
AXG97319.1\|3583553_3584717_-\|integrase	site-specific integrase	A0A2H4JCB7	uncultured_Caudovirales_phage	5.3e-35	31.4
AXG97320.1\|3584765_3585218_-	ImmA/IrrE family metallo-endopeptidase	I3VYZ2	Thermoanaerobacterium_phage	6.2e-16	40.0
AXG97321.1\|3585226_3585721_-	helix-turn-helix domain-containing protein	M9Q2L3	Clostridium_phage	1.4e-13	39.1
AXG97322.1\|3585878_3586073_+	XRE family transcriptional regulator	A0A0A8WE47	Clostridium_phage	6.1e-05	36.5
AXG97323.1\|3586090_3586351_+	helix-turn-helix domain-containing protein	NA	NA	NA	NA
AXG97324.1\|3586365_3586545_+	hypothetical protein	NA	NA	NA	NA
AXG97325.1\|3586755_3587040_+	hypothetical protein	A0A0A7RTW4	Clostridium_phage	1.1e-21	63.8
AXG97326.1\|3587092_3587446_+	hypothetical protein	A0A0A7RVM0	Clostridium_phage	3.4e-38	69.8
AXG97327.1\|3587463_3587709_+	hypothetical protein	A0A0A7RTK9	Clostridium_phage	1.1e-24	65.0
AXG97328.1\|3587742_3588192_+	hypothetical protein	A0A0A7RTD8	Clostridium_phage	1.9e-09	50.9
AXG97329.1\|3588191_3589337_+	DUF2800 domain-containing protein	A0A0A7S066	Clostridium_phage	5.0e-195	88.5
AXG97330.1\|3589348_3589969_+	DUF2815 family protein	A0A0A7RVM4	Clostridium_phage	6.8e-90	85.5
AXG97331.1\|3590139_3592101_+	DNA polymerase I	A0A0A7RTL3	Clostridium_phage	3.3e-279	72.4
AXG97332.1\|3592139_3592370_+	glutamate racemase	NA	NA	NA	NA
AXG97333.1\|3592406_3593432_+	nucleoid-associated protein	A0A090D855	Clostridium_phage	1.9e-44	33.1
AXG97334.1\|3593477_3595904_+	hypothetical protein	A0A0A7RTG3	Clostridium_phage	5.4e-276	60.6
AXG97335.1\|3596298_3596574_+	VRR-NUC domain-containing protein	A0A0A7RTE1	Clostridium_phage	5.4e-23	86.7
AXG97336.1\|3597999_3598479_+	hypothetical protein	I2E8Y5	Clostridium_phage	2.7e-25	43.7
AXG97337.1\|3599110_3599455_+	hypothetical protein	NA	NA	NA	NA
AXG97338.1\|3599521_3600115_+\|terminase	terminase small subunit	U5PZD3	Bacillus_phage	1.4e-20	36.8
AXG97339.1\|3600107_3601517_+\|terminase	terminase	A0A0A7RTS1	Clostridium_phage	3.6e-211	87.2
AXG97725.1\|3601530_3602982_+\|portal	phage portal protein	A0A0A7S074	Clostridium_phage	4.4e-233	85.8
AXG97340.1\|3602962_3604003_+\|head	phage head morphogenesis protein	A0A0A7RVY7	Clostridium_phage	2.3e-146	75.7
AXG97341.1\|3604099_3604681_+	hypothetical protein	NA	NA	NA	NA
AXG97342.1\|3604748_3605108_+	ABC transporter ATPase	A0A2H4J4N9	uncultured_Caudovirales_phage	2.0e-49	81.5
AXG97343.1\|3605130_3605703_+	scaffolding protein	A0A0A7RTM5	Clostridium_phage	1.7e-39	54.7
AXG97344.1\|3605725_3606793_+\|coat	phage coat protein	A0A0A7RTH8	Clostridium_phage	2.5e-180	88.5
AXG97726.1\|3606812_3607157_+	hypothetical protein	A0A0A7RTX9	Clostridium_phage	2.6e-43	75.4
AXG97345.1\|3607158_3607542_+	hypothetical protein	A0A0A7S083	Clostridium_phage	3.7e-54	83.5
AXG97346.1\|3607541_3608033_+	HK97 gp10 family phage protein	A0A0A7RTT0	Clostridium_phage	2.1e-62	75.3
AXG97347.1\|3608034_3608466_+	hypothetical protein	A0A0A7RTI2	Clostridium_phage	5.1e-52	75.4
AXG97348.1\|3608627_3609938_+\|tail	phage tail sheath protein	A0A0A7S087	Clostridium_phage	1.3e-212	85.1
AXG97349.1\|3609941_3610406_+\|portal	phage portal protein	A0A0A7RVP1	Clostridium_phage	1.1e-68	83.1
AXG97350.1\|3610421_3610835_+	hypothetical protein	A0A0A7RTN3	Clostridium_phage	2.1e-55	77.0
AXG97727.1\|3610834_3611029_+	hypothetical protein	NA	NA	NA	NA
AXG97351.1\|3611089_3611851_+	SHOCT domain-containing protein	X5JB37	Clostridium_phage	2.6e-43	42.8
AXG97352.1\|3611908_3614068_+	hypothetical protein	A0A0A7S091	Clostridium_phage	1.6e-133	48.0
AXG97353.1\|3614067_3614745_+	LysM peptidoglycan-binding domain-containing protein	A0A0A7RVP5	Clostridium_phage	4.6e-92	75.0
AXG97354.1\|3614756_3615731_+	hypothetical protein	A0A0A7RTZ4	Clostridium_phage	2.7e-157	84.9
AXG97355.1\|3615733_3616078_+	DUF2577 domain-containing protein	A0A0A7RTJ2	Clostridium_phage	7.4e-38	74.6
AXG97356.1\|3616080_3616488_+	DUF2634 domain-containing protein	A0A0A7RTH1	Clostridium_phage	1.1e-51	78.5
AXG97357.1\|3616488_3617583_+\|plate	baseplate J/gp47 family protein	A0A0A7S096	Clostridium_phage	1.3e-160	76.1
AXG97358.1\|3617563_3618187_+	DUF2313 domain-containing protein	A0A0A7RVP9	Clostridium_phage	2.4e-79	71.4
AXG97359.1\|3618189_3619929_+\|tail	phage tail protein	A0A0A7RTQ0	Clostridium_phage	1.9e-60	51.1
AXG97360.1\|3619961_3620354_+	hypothetical protein	B6SBV4	Clostridium_virus	4.5e-07	37.6
AXG97361.1\|3620346_3620469_+	XkdX family protein	A0A0A7S0E7	Clostridium_phage	4.5e-06	57.5
AXG97728.1\|3620792_3621989_+\|transposase	IS110 family transposase	A0A0U3ULR1	Bacillus_phage	4.7e-87	44.4
AXG97362.1\|3622156_3622576_+	hypothetical protein	A0A0A7S099	Clostridium_phage	1.1e-43	66.2
AXG97363.1\|3622620_3623391_+	N-acetylmuramoyl-L-alanine amidase	A0A2H4J8A3	uncultured_Caudovirales_phage	2.2e-90	66.1
AXG97364.1\|3623569_3625138_+	hypothetical protein	NA	NA	NA	NA
AXG97365.1\|3625226_3626090_+	DNA adenine methylase	NA	NA	NA	NA
AXG97366.1\|3626940_3627129_+	hypothetical protein	A0A0A7RTH9	Clostridium_phage	2.9e-12	70.9
AXG97367.1\|3627130_3627373_+	hypothetical protein	NA	NA	NA	NA
AXG97368.1\|3628701_3630216_+	DUF1538 domain-containing protein	NA	NA	NA	NA
3627822:3627882	attR	NA	NA	NA	NA
AXG97369.1\|3630723_3633318_+	cation-transporting P-type ATPase	NA	NA	NA	NA
AXG97370.1\|3633422_3633653_+	hypothetical protein	NA	NA	NA	NA
AXG97371.1\|3633664_3635452_+	ComEC/Rec2 family competence protein	NA	NA	NA	NA
AXG97372.1\|3635480_3636512_+	DNA polymerase III subunit delta	NA	NA	NA	NA
AXG97373.1\|3636546_3636813_-	30S ribosomal protein S20	NA	NA	NA	NA
AXG97374.1\|3637022_3637997_+	GPR endopeptidase	NA	NA	NA	NA
AXG97375.1\|3638181_3639273_+	stage II sporulation protein P	NA	NA	NA	NA
AXG97376.1\|3639343_3639739_+	hypothetical protein	NA	NA	NA	NA
AXG97377.1\|3639879_3641688_+	elongation factor 4	E4ZFJ7	Streptococcus_phage	4.2e-23	25.3
AXG97378.1\|3641714_3642857_+	oxygen-independent coproporphyrinogen III oxidase	NA	NA	NA	NA
AXG97379.1\|3643057_3644089_+	heat-inducible transcriptional repressor HrcA	NA	NA	NA	NA
AXG97380.1\|3644115_3644760_+	nucleotide exchange factor GrpE	NA	NA	NA	NA
AXG97381.1\|3644814_3646686_+	molecular chaperone DnaK	A0A2P1EIW2	Megavirus	1.6e-142	46.7
AXG97382.1\|3646828_3647974_+	molecular chaperone DnaJ	A0A1V0SBY2	Catovirus	2.2e-25	29.0
AXG97383.1\|3648281_3649220_+	50S ribosomal protein L11 methyltransferase	NA	NA	NA	NA
AXG97384.1\|3649317_3650076_+	16S rRNA (uracil(1498)-N(3))-methyltransferase	NA	NA	NA	NA
AXG97385.1\|3650075_3651374_+\|tRNA	tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))- methylthiotransferase MtaB	NA	NA	NA	NA

DBSCAN-SWA_5

3728989 : 3738473

Synechococcus_phage(42.86%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AXG97454.1\|3728989_3731560_+	selenium-dependent xanthine dehydrogenase	A0A0P0IVM8	Acinetobacter_phage	1.3e-09	32.4
AXG97455.1\|3731580_3731715_-	hypothetical protein	NA	NA	NA	NA
AXG97456.1\|3732235_3732715_+	5-(carboxyamino)imidazole ribonucleotide mutase	A0A2P0VNU7	Tetraselmis_virus	1.1e-26	48.7
AXG97457.1\|3732714_3733419_+	phosphoribosylaminoimidazolesuccinocarboxamide synthase	G8EYA2	Synechococcus_phage	1.0e-41	42.1
AXG97458.1\|3733510_3734959_+	amidophosphoribosyltransferase	A0A0M3SGR2	Mollivirus	7.2e-58	35.1
AXG97459.1\|3735019_3736015_+	phosphoribosylformylglycinamidine cyclo-ligase	M4QRQ6	Synechococcus_phage	3.4e-67	44.0
AXG97460.1\|3736142_3736760_+	phosphoribosylglycinamide formyltransferase	M4QRX9	Synechococcus_phage	1.5e-25	40.6
AXG97461.1\|3736973_3738473_+	bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/inosine monophosphate cyclohydrolase	Q58MG4	Prochlorococcus_phage	1.1e-69	46.9

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

Overview of predicted results

Overview of the results

Cas Category Instructions

Results visualization

1. CP031100

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection

2. CP031098

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection