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Overview of predicted results

Overview of the results

Contig_ID Contig_def CRISPR array number Contig Signature genes Self targeting spacer number Target MGE spacer number Prophage number Anti-CRISPR protein number
CP022910 Staphylococcus aureus strain 164 chromosome, complete genome 8 crisprs cas3,DEDDh,DinG,csa3,WYL 8 1 8 0
CP022911 Staphylococcus aureus strain 164 plasmid pPS00089.1A.1, complete sequence 0 crisprs RT,csa3 0 0 0 0

Results visualization

1. CP022910
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CRISPR_ID CRISPR_location CRISPR_type Repeat_type Spacer_info Cas_protein_info CRISPR-Cas_info
CP022910_1 374173-374273 Orphan NA
1 spacers

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CRISPR_ID CRISPR_location CRISPR_type Repeat_type Spacer_info Cas_protein_info CRISPR-Cas_info
CP022910_2 618357-618449 Orphan NA
1 spacers

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CRISPR_ID CRISPR_location CRISPR_type Repeat_type Spacer_info Cas_protein_info CRISPR-Cas_info
CP022910_3 781200-781285 Unclear NA
1 spacers

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CRISPR_ID CRISPR_location CRISPR_type Repeat_type Spacer_info Cas_protein_info CRISPR-Cas_info
CP022910_4 824530-824611 Orphan NA
1 spacers

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CRISPR_ID CRISPR_location CRISPR_type Repeat_type Spacer_info Cas_protein_info CRISPR-Cas_info
CP022910_5 1022931-1023018 Orphan NA
1 spacers

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CRISPR_ID CRISPR_location CRISPR_type Repeat_type Spacer_info Cas_protein_info CRISPR-Cas_info
CP022910_6 1807785-1807995 Orphan NA
3 spacers

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CRISPR_ID CRISPR_location CRISPR_type Repeat_type Spacer_info Cas_protein_info CRISPR-Cas_info
CP022910_7 2199391-2199541 Orphan NA
2 spacers
csa3

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CRISPR_ID CRISPR_location CRISPR_type Repeat_type Spacer_info Cas_protein_info CRISPR-Cas_info
CP022910_8 2323547-2323740 Orphan NA
3 spacers

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CRISPR_ID Spacer_Info Spacer_region Spacer_length Hit_ID Protospacer_location Mismatch Identity
CP022910_4 4.1|824554|34|CP022910|CRISPRCasFinder 824554-824587 34 CP022910.1 1315405-1315438 0 1.0
CP022910_4 4.1|824554|34|CP022910|CRISPRCasFinder 824554-824587 34 CP022910.1 1404592-1404625 0 1.0
CP022910_6 6.2|1807882|20|CP022910|CRT 1807882-1807901 20 CP022910.1 114777-114796 0 1.0
CP022910_6 6.2|1807882|20|CP022910|CRT 1807882-1807901 20 CP022910.1 257974-257993 0 1.0
CP022910_6 6.2|1807882|20|CP022910|CRT 1807882-1807901 20 CP022910.1 335692-335711 0 1.0
CP022910_6 6.2|1807882|20|CP022910|CRT 1807882-1807901 20 CP022910.1 775730-775749 0 1.0
CP022910_6 6.2|1807882|20|CP022910|CRT 1807882-1807901 20 CP022910.1 775785-775804 0 1.0
CP022910_6 6.2|1807882|20|CP022910|CRT 1807882-1807901 20 CP022910.1 775840-775859 0 1.0
CP022910_6 6.2|1807882|20|CP022910|CRT 1807882-1807901 20 CP022910.1 781389-781408 0 1.0
CP022910_6 6.2|1807882|20|CP022910|CRT 1807882-1807901 20 CP022910.1 781447-781466 0 1.0
CP022910_6 6.2|1807882|20|CP022910|CRT 1807882-1807901 20 CP022910.1 824608-824627 0 1.0
CP022910_6 6.2|1807882|20|CP022910|CRT 1807882-1807901 20 CP022910.1 908942-908961 0 1.0
CP022910_6 6.2|1807882|20|CP022910|CRT 1807882-1807901 20 CP022910.1 1032914-1032933 0 1.0
CP022910_6 6.2|1807882|20|CP022910|CRT 1807882-1807901 20 CP022910.1 1315306-1315325 0 1.0
CP022910_6 6.2|1807882|20|CP022910|CRT 1807882-1807901 20 CP022910.1 1404705-1404724 0 1.0
CP022910_6 6.2|1807882|20|CP022910|CRT 1807882-1807901 20 CP022910.1 1629167-1629186 0 1.0
CP022910_6 6.2|1807882|20|CP022910|CRT 1807882-1807901 20 CP022910.1 1807709-1807728 0 1.0
CP022910_6 6.2|1807882|20|CP022910|CRT 1807882-1807901 20 CP022910.1 1965749-1965768 0 1.0
CP022910_6 6.2|1807882|20|CP022910|CRT 1807882-1807901 20 CP022910.1 2515150-2515169 0 1.0
CP022910_6 6.2|1807882|20|CP022910|CRT 1807882-1807901 20 CP022910.1 2764670-2764689 0 1.0
CP022910_8 8.2|2323626|36|CP022910|CRT 2323626-2323661 36 CP022910.1 1107203-1107238 0 1.0
CP022910_3 3.1|781230|26|CP022910|CRISPRCasFinder 781230-781255 26 CP022910.1 744112-744137 1 0.962
CP022910_3 3.1|781230|26|CP022910|CRISPRCasFinder 781230-781255 26 CP022910.1 2764570-2764595 1 0.962
CP022910_3 3.1|781230|26|CP022910|CRISPRCasFinder 781230-781255 26 CP022910.1 2764626-2764651 1 0.962
CP022910_6 6.1|1807823|21|CP022910|CRT 1807823-1807843 21 CP022910.1 1807764-1807784 1 0.952
CP022910_6 6.2|1807882|20|CP022910|CRT 1807882-1807901 20 CP022910.1 258029-258048 1 0.95
CP022910_6 6.2|1807882|20|CP022910|CRT 1807882-1807901 20 CP022910.1 788656-788675 1 0.95
CP022910_6 6.2|1807882|20|CP022910|CRT 1807882-1807901 20 CP022910.1 1107207-1107226 1 0.95
CP022910_6 6.2|1807882|20|CP022910|CRT 1807882-1807901 20 CP022910.1 1364632-1364651 1 0.95
CP022910_6 6.2|1807882|20|CP022910|CRT 1807882-1807901 20 CP022910.1 1663130-1663149 1 0.95
CP022910_6 6.2|1807882|20|CP022910|CRT 1807882-1807901 20 CP022910.1 1975157-1975176 1 0.95
CP022910_6 6.3|1807940|18|CP022910|CRT 1807940-1807957 18 CP022910.1 1807767-1807784 1 0.944
CP022910_8 8.2|2323626|36|CP022910|CRT 2323626-2323661 36 CP022910.1 1807705-1807740 1 0.972
CP022910_3 3.1|781230|26|CP022910|CRISPRCasFinder 781230-781255 26 CP022910.1 744168-744193 2 0.923
CP022910_3 3.1|781230|26|CP022910|CRISPRCasFinder 781230-781255 26 CP022910.1 889293-889318 2 0.923
CP022910_3 3.1|781230|26|CP022910|CRISPRCasFinder 781230-781255 26 CP022910.1 889349-889374 2 0.923
CP022910_6 6.2|1807882|20|CP022910|CRT 1807882-1807901 20 CP022910.1 748712-748731 2 0.9
CP022910_8 8.1|2323570|33|CP022910|CRT 2323570-2323602 33 CP022910.1 781266-781298 2 0.939
CP022910_8 8.1|2323570|33|CP022910|CRT 2323570-2323602 33 CP022910.1 1107259-1107291 2 0.939
CP022910_8 8.1|2323570|33|CP022910|CRT 2323570-2323602 33 CP022910.1 1315360-1315392 2 0.939
CP022910_8 8.1|2323570|33|CP022910|CRT 2323570-2323602 33 CP022910.1 1404638-1404670 2 0.939
CP022910_8 8.1|2323570|33|CP022910|CRT 2323570-2323602 33 CP022910.1 2599063-2599095 2 0.939
CP022910_8 8.2|2323626|36|CP022910|CRT 2323626-2323661 36 CP022910.1 1629155-1629190 2 0.944
CP022910_8 8.2|2323626|36|CP022910|CRT 2323626-2323661 36 CP022910.1 1975145-1975180 2 0.944
CP022910_8 8.3|2323685|33|CP022910|CRT 2323685-2323717 33 CP022910.1 781266-781298 2 0.939
CP022910_8 8.3|2323685|33|CP022910|CRT 2323685-2323717 33 CP022910.1 1107259-1107291 2 0.939
CP022910_8 8.3|2323685|33|CP022910|CRT 2323685-2323717 33 CP022910.1 1315360-1315392 2 0.939
CP022910_8 8.3|2323685|33|CP022910|CRT 2323685-2323717 33 CP022910.1 2599063-2599095 2 0.939

1. spacer 4.1|824554|34|CP022910|CRISPRCasFinder matches to position: 1315405-1315438, mismatch: 0, identity: 1.0

attgggaatccaatttctctttgttggggcccat	CRISPR spacer
attgggaatccaatttctctttgttggggcccat	Protospacer
**********************************

2. spacer 4.1|824554|34|CP022910|CRISPRCasFinder matches to position: 1404592-1404625, mismatch: 0, identity: 1.0

attgggaatccaatttctctttgttggggcccat	CRISPR spacer
attgggaatccaatttctctttgttggggcccat	Protospacer
**********************************

3. spacer 6.2|1807882|20|CP022910|CRT matches to position: 114777-114796, mismatch: 0, identity: 1.0

gaatttcgaaaagaaattct	CRISPR spacer
gaatttcgaaaagaaattct	Protospacer
********************

4. spacer 6.2|1807882|20|CP022910|CRT matches to position: 257974-257993, mismatch: 0, identity: 1.0

gaatttcgaaaagaaattct	CRISPR spacer
gaatttcgaaaagaaattct	Protospacer
********************

5. spacer 6.2|1807882|20|CP022910|CRT matches to position: 335692-335711, mismatch: 0, identity: 1.0

gaatttcgaaaagaaattct	CRISPR spacer
gaatttcgaaaagaaattct	Protospacer
********************

6. spacer 6.2|1807882|20|CP022910|CRT matches to position: 775730-775749, mismatch: 0, identity: 1.0

gaatttcgaaaagaaattct	CRISPR spacer
gaatttcgaaaagaaattct	Protospacer
********************

7. spacer 6.2|1807882|20|CP022910|CRT matches to position: 775785-775804, mismatch: 0, identity: 1.0

gaatttcgaaaagaaattct	CRISPR spacer
gaatttcgaaaagaaattct	Protospacer
********************

8. spacer 6.2|1807882|20|CP022910|CRT matches to position: 775840-775859, mismatch: 0, identity: 1.0

gaatttcgaaaagaaattct	CRISPR spacer
gaatttcgaaaagaaattct	Protospacer
********************

9. spacer 6.2|1807882|20|CP022910|CRT matches to position: 781389-781408, mismatch: 0, identity: 1.0

gaatttcgaaaagaaattct	CRISPR spacer
gaatttcgaaaagaaattct	Protospacer
********************

10. spacer 6.2|1807882|20|CP022910|CRT matches to position: 781447-781466, mismatch: 0, identity: 1.0

gaatttcgaaaagaaattct	CRISPR spacer
gaatttcgaaaagaaattct	Protospacer
********************

11. spacer 6.2|1807882|20|CP022910|CRT matches to position: 824608-824627, mismatch: 0, identity: 1.0

gaatttcgaaaagaaattct	CRISPR spacer
gaatttcgaaaagaaattct	Protospacer
********************

12. spacer 6.2|1807882|20|CP022910|CRT matches to position: 908942-908961, mismatch: 0, identity: 1.0

gaatttcgaaaagaaattct	CRISPR spacer
gaatttcgaaaagaaattct	Protospacer
********************

13. spacer 6.2|1807882|20|CP022910|CRT matches to position: 1032914-1032933, mismatch: 0, identity: 1.0

gaatttcgaaaagaaattct	CRISPR spacer
gaatttcgaaaagaaattct	Protospacer
********************

14. spacer 6.2|1807882|20|CP022910|CRT matches to position: 1315306-1315325, mismatch: 0, identity: 1.0

gaatttcgaaaagaaattct	CRISPR spacer
gaatttcgaaaagaaattct	Protospacer
********************

15. spacer 6.2|1807882|20|CP022910|CRT matches to position: 1404705-1404724, mismatch: 0, identity: 1.0

gaatttcgaaaagaaattct	CRISPR spacer
gaatttcgaaaagaaattct	Protospacer
********************

16. spacer 6.2|1807882|20|CP022910|CRT matches to position: 1629167-1629186, mismatch: 0, identity: 1.0

gaatttcgaaaagaaattct	CRISPR spacer
gaatttcgaaaagaaattct	Protospacer
********************

17. spacer 6.2|1807882|20|CP022910|CRT matches to position: 1807709-1807728, mismatch: 0, identity: 1.0

gaatttcgaaaagaaattct	CRISPR spacer
gaatttcgaaaagaaattct	Protospacer
********************

18. spacer 6.2|1807882|20|CP022910|CRT matches to position: 1965749-1965768, mismatch: 0, identity: 1.0

gaatttcgaaaagaaattct	CRISPR spacer
gaatttcgaaaagaaattct	Protospacer
********************

19. spacer 6.2|1807882|20|CP022910|CRT matches to position: 2515150-2515169, mismatch: 0, identity: 1.0

gaatttcgaaaagaaattct	CRISPR spacer
gaatttcgaaaagaaattct	Protospacer
********************

20. spacer 6.2|1807882|20|CP022910|CRT matches to position: 2764670-2764689, mismatch: 0, identity: 1.0

gaatttcgaaaagaaattct	CRISPR spacer
gaatttcgaaaagaaattct	Protospacer
********************

21. spacer 8.2|2323626|36|CP022910|CRT matches to position: 1107203-1107238, mismatch: 0, identity: 1.0

tgcattgtctgtagaatttctttttgaaattctcta	CRISPR spacer
tgcattgtctgtagaatttctttttgaaattctcta	Protospacer
************************************

22. spacer 3.1|781230|26|CP022910|CRISPRCasFinder matches to position: 744112-744137, mismatch: 1, identity: 0.962

ccgccagcttctgtgttggggccccg	CRISPR spacer
ccgcctgcttctgtgttggggccccg	Protospacer
***** ********************

23. spacer 3.1|781230|26|CP022910|CRISPRCasFinder matches to position: 2764570-2764595, mismatch: 1, identity: 0.962

ccgccagcttctgtgttggggccccg	CRISPR spacer
ccgccagcttctatgttggggccccg	Protospacer
************.*************

24. spacer 3.1|781230|26|CP022910|CRISPRCasFinder matches to position: 2764626-2764651, mismatch: 1, identity: 0.962

ccgccagcttctgtgttggggccccg	CRISPR spacer
ccgccagcttctttgttggggccccg	Protospacer
************ *************

25. spacer 6.1|1807823|21|CP022910|CRT matches to position: 1807764-1807784, mismatch: 1, identity: 0.952

agctggccaatagtcagcttt	CRISPR spacer
agctggccaatagttagcttt	Protospacer
**************.******

26. spacer 6.2|1807882|20|CP022910|CRT matches to position: 258029-258048, mismatch: 1, identity: 0.95

gaatttcgaaaagaaattct	CRISPR spacer
gaaattcgaaaagaaattct	Protospacer
*** ****************

27. spacer 6.2|1807882|20|CP022910|CRT matches to position: 788656-788675, mismatch: 1, identity: 0.95

gaatttcgaaaagaaattct	CRISPR spacer
gaatttcgaaatgaaattct	Protospacer
*********** ********

28. spacer 6.2|1807882|20|CP022910|CRT matches to position: 1107207-1107226, mismatch: 1, identity: 0.95

gaatttcgaaaagaaattct	CRISPR spacer
gaatttcaaaaagaaattct	Protospacer
*******.************

29. spacer 6.2|1807882|20|CP022910|CRT matches to position: 1364632-1364651, mismatch: 1, identity: 0.95

gaatttcgaaaagaaattct	CRISPR spacer
gattttcgaaaagaaattct	Protospacer
** *****************

30. spacer 6.2|1807882|20|CP022910|CRT matches to position: 1663130-1663149, mismatch: 1, identity: 0.95

gaatttcgaaaagaaattct	CRISPR spacer
gaatttcgtaaagaaattct	Protospacer
******** ***********

31. spacer 6.2|1807882|20|CP022910|CRT matches to position: 1975157-1975176, mismatch: 1, identity: 0.95

gaatttcgaaaagaaattct	CRISPR spacer
gaatttcgaaaggaaattct	Protospacer
***********.********

32. spacer 6.3|1807940|18|CP022910|CRT matches to position: 1807767-1807784, mismatch: 1, identity: 0.944

tggccaatagtcagcttt	CRISPR spacer
tggccaatagttagcttt	Protospacer
***********.******

33. spacer 8.2|2323626|36|CP022910|CRT matches to position: 1807705-1807740, mismatch: 1, identity: 0.972

tgcattgtctgtagaatttctttttgaaattctcta	CRISPR spacer
tgcattgtctgtagaatttcttttcgaaattctcta	Protospacer
************************.***********

34. spacer 3.1|781230|26|CP022910|CRISPRCasFinder matches to position: 744168-744193, mismatch: 2, identity: 0.923

ccgccagcttctgtgttggggccccg	CRISPR spacer
ccgcctgcttctatgttggggccccg	Protospacer
***** ******.*************

35. spacer 3.1|781230|26|CP022910|CRISPRCasFinder matches to position: 889293-889318, mismatch: 2, identity: 0.923

ccgccagcttctgtgttggggccccg	CRISPR spacer
ccaccagcctctgtgttggggccccg	Protospacer
**.*****.*****************

36. spacer 3.1|781230|26|CP022910|CRISPRCasFinder matches to position: 889349-889374, mismatch: 2, identity: 0.923

ccgccagcttctgtgttggggccccg	CRISPR spacer
ccaccagcctctgtgttggggccccg	Protospacer
**.*****.*****************

37. spacer 6.2|1807882|20|CP022910|CRT matches to position: 748712-748731, mismatch: 2, identity: 0.9

gaatttcgaaaagaaattct	CRISPR spacer
gaatttcaaaaaggaattct	Protospacer
*******.*****.******

38. spacer 8.1|2323570|33|CP022910|CRT matches to position: 781266-781298, mismatch: 2, identity: 0.939

cattattgtatgctgacttttcgtcaccttctg	CRISPR spacer
cattattgtaagctgacttttcgtcagcttctg	Protospacer
********** *************** ******

39. spacer 8.1|2323570|33|CP022910|CRT matches to position: 1107259-1107291, mismatch: 2, identity: 0.939

cattattgtatgctgacttttcgtcaccttctg	CRISPR spacer
cattattgtaagctgacttttcgtcagcttctg	Protospacer
********** *************** ******

40. spacer 8.1|2323570|33|CP022910|CRT matches to position: 1315360-1315392, mismatch: 2, identity: 0.939

cattattgtatgctgacttttcgtcaccttctg	CRISPR spacer
cattattgtaagctgacttttcgtcagcttctg	Protospacer
********** *************** ******

41. spacer 8.1|2323570|33|CP022910|CRT matches to position: 1404638-1404670, mismatch: 2, identity: 0.939

cattattgtatgctgacttttcgtcaccttctg	CRISPR spacer
cattattgtaagctgacttttcgtcatcttctg	Protospacer
********** ***************.******

42. spacer 8.1|2323570|33|CP022910|CRT matches to position: 2599063-2599095, mismatch: 2, identity: 0.939

cattattgtatgctgacttttcgtcaccttctg	CRISPR spacer
cattattgtaagctgacttttcgtcagcttctg	Protospacer
********** *************** ******

43. spacer 8.2|2323626|36|CP022910|CRT matches to position: 1629155-1629190, mismatch: 2, identity: 0.944

tgcattgtctgtagaatttctttttgaaattctcta	CRISPR spacer
tgcattgcctgtagaatttcttttcgaaattctcta	Protospacer
*******.****************.***********

44. spacer 8.2|2323626|36|CP022910|CRT matches to position: 1975145-1975180, mismatch: 2, identity: 0.944

tgcattgtctgtagaatttctttttgaaattctcta	CRISPR spacer
tgcattgtctgtagaatttcctttcgaaattctcta	Protospacer
********************.***.***********

45. spacer 8.3|2323685|33|CP022910|CRT matches to position: 781266-781298, mismatch: 2, identity: 0.939

cattattgtaagctgactttctgtcagcttctg	CRISPR spacer
cattattgtaagctgacttttcgtcagcttctg	Protospacer
********************..***********

46. spacer 8.3|2323685|33|CP022910|CRT matches to position: 1107259-1107291, mismatch: 2, identity: 0.939

cattattgtaagctgactttctgtcagcttctg	CRISPR spacer
cattattgtaagctgacttttcgtcagcttctg	Protospacer
********************..***********

47. spacer 8.3|2323685|33|CP022910|CRT matches to position: 1315360-1315392, mismatch: 2, identity: 0.939

cattattgtaagctgactttctgtcagcttctg	CRISPR spacer
cattattgtaagctgacttttcgtcagcttctg	Protospacer
********************..***********

48. spacer 8.3|2323685|33|CP022910|CRT matches to position: 2599063-2599095, mismatch: 2, identity: 0.939

cattattgtaagctgactttctgtcagcttctg	CRISPR spacer
cattattgtaagctgacttttcgtcagcttctg	Protospacer
********************..***********

CRISPR_ID Spacer_Info Spacer_region Spacer_length Hit_phage_ID Hit_phage_def Protospacer_location Mismatch Identity
CP022910_3 3.1|781230|26|CP022910|CRISPRCasFinder 781230-781255 26 NZ_CP022541 Antarctobacter heliothermus strain SMS3 plasmid pSMS3-1, complete sequence 333735-333760 5 0.808
CP022910_3 3.1|781230|26|CP022910|CRISPRCasFinder 781230-781255 26 NZ_CP034332 Tabrizicola piscis strain K13M18 plasmid unnamed4, complete sequence 3359-3384 5 0.808
CP022910_3 3.1|781230|26|CP022910|CRISPRCasFinder 781230-781255 26 NZ_CP022605 Ochrobactrum quorumnocens strain A44 plasmid unnamed1, complete sequence 715511-715536 5 0.808

1. spacer 3.1|781230|26|CP022910|CRISPRCasFinder matches to NZ_CP022541 (Antarctobacter heliothermus strain SMS3 plasmid pSMS3-1, complete sequence) position: , mismatch: 5, identity: 0.808

ccgccagcttctgtgttggggccccg	CRISPR spacer
acgccagcttctgtgttgaggcctta	Protospacer
 *****************.****...

2. spacer 3.1|781230|26|CP022910|CRISPRCasFinder matches to NZ_CP034332 (Tabrizicola piscis strain K13M18 plasmid unnamed4, complete sequence) position: , mismatch: 5, identity: 0.808

ccgccagcttctgtgttggggccccg	CRISPR spacer
ccgccagcttctgtgtctgggcctac	Protospacer
****************. *****.  

3. spacer 3.1|781230|26|CP022910|CRISPRCasFinder matches to NZ_CP022605 (Ochrobactrum quorumnocens strain A44 plasmid unnamed1, complete sequence) position: , mismatch: 5, identity: 0.808

ccgccagcttctgtgttggggccccg	CRISPR spacer
ccgccagcttctgtgtttggccctga	Protospacer
***************** ** **. .

Region Region Position Protein_number Hit_taxonomy Key_proteins Att_site Prophage annotation
DBSCAN-SWA_1 712813 : 720633 10 Hokovirus(16.67%) NA NA
DBSCAN-SWA_2 732606 : 747326 12 uncultured_Caudovirales_phage(50.0%) NA NA
DBSCAN-SWA_3 826092 : 847300 33 Staphylococcus_phage(77.78%) NA NA
DBSCAN-SWA_4 1458595 : 1545654 102 Staphylococcus_phage(64.94%) protease,portal,holin,capsid,tRNA,terminase,head,lysis,tail NA
DBSCAN-SWA_5 1600778 : 1609090 7 Staphylococcus_phage(16.67%) tRNA NA
DBSCAN-SWA_6 1678224 : 1687267 7 uncultured_Mediterranean_phage(50.0%) tRNA NA
DBSCAN-SWA_7 1812882 : 1894354 70 Staphylococcus_phage(93.44%) tRNA,protease NA
DBSCAN-SWA_8 1975801 : 2065612 110 Staphylococcus_phage(88.75%) protease,portal,holin,capsid,terminase,head,tRNA,tail NA
Acr ID Acr position Acr size Homology with known anti Neighbor HTH/AcRanker Neighbor Aca In prophage Protospacer in prophage