CRISPRimmunity

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Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Self targeting spacer number	Target MGE spacer number	Prophage number	Anti-CRISPR protein number
CP022761	Ralstonia solanacearum strain T95 chromosome, complete genome	0 crisprs	cas3,WYL,csa3,DEDDh,DinG	0	0	5	0
CP022762	Ralstonia solanacearum strain T95 plasmid unnamed, complete sequence	0 crisprs	csa3,PD-DExK	0	0	0	0

Results visualization

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CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

204999 : 218794

Acidithiobacillus_phage(75.0%)

The bacterium proteins that are colored denote the protein is present at specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin' and 'tRNA')

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AXV87850.1\|204999_205983_-	ATP-binding protein	R4TQL5	Phaeocystis_globosa_virus	5.1e-15	27.7
AXV84988.1\|206739_208977_-	hypothetical protein	K4I1D0	Acidithiobacillus_phage	1.1e-291	66.5
AXV84989.1\|209026_209488_-	excisionase	K4ICM4	Acidithiobacillus_phage	3.4e-46	63.4
AXV84990.1\|209715_209979_+	DNA-binding protein	K4I3X3	Acidithiobacillus_phage	5.3e-20	69.3
AXV84991.1\|209989_210472_+	hypothetical protein	K4HZ96	Acidithiobacillus_phage	3.4e-44	58.2
AXV84992.1\|210779_211232_+	HNH endonuclease	A0A1B0TRC1	Escherichia_phage	3.4e-22	39.3
AXV84993.1\|211218_211977_+	hypothetical protein	K4HZA0	Acidithiobacillus_phage	2.9e-82	61.7
AXV84994.1\|211973_212234_+	hypothetical protein	K4I1D6	Acidithiobacillus_phage	8.4e-18	76.8
AXV84995.1\|212230_214519_+	hypothetical protein	K4HZY1	Acidithiobacillus_phage	1.4e-286	63.5
AXV84996.1\|214649_215114_+	hypothetical protein	K4ICN3	Acidithiobacillus_phage	1.0e-53	67.6
AXV84997.1\|215115_215325_+	hypothetical protein	NA	NA	NA	NA
AXV84998.1\|215317_215701_+	RNA polymerase subunit sigma	NA	NA	NA	NA
AXV84999.1\|215755_216022_+	type II toxin-antitoxin system ParD family antitoxin	NA	NA	NA	NA
AXV85000.1\|216021_216321_+	type II toxin-antitoxin system RelE/ParE family toxin	NA	NA	NA	NA
AXV85001.1\|216274_216490_+	hypothetical protein	NA	NA	NA	NA
AXV85002.1\|216423_216720_-	hypothetical protein	NA	NA	NA	NA
AXV87851.1\|216854_217400_+	hypothetical protein	A0A2H4P763	Pseudomonas_phage	2.0e-24	39.6
AXV85003.1\|217381_218794_+	DNA modification methylase	K4I3Y2	Acidithiobacillus_phage	1.3e-152	59.3

DBSCAN-SWA_2

223258 : 271135

uncultured_Caudovirales_phage(20.0%)

protease,terminase,portal,capsid,transposase,head

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AXV85007.1\|223258_223735_-	DUF3489 domain-containing protein	A0A2H4JE21	uncultured_Caudovirales_phage	5.5e-23	60.0
AXV85008.1\|224551_224773_-	hypothetical protein	NA	NA	NA	NA
AXV85009.1\|224870_225407_+	elements of external origin	A0A2H4JCL4	uncultured_Caudovirales_phage	1.5e-72	82.6
AXV87852.1\|225409_227377_+\|terminase	terminase	A0A2H4JIC6	uncultured_Caudovirales_phage	0.0e+00	85.8
AXV85010.1\|227420_227930_+	hypothetical protein	A0A0F6WCR7	Sinorhizobium_phage	2.8e-25	44.4
AXV85011.1\|227940_228333_+	hypothetical protein	NA	NA	NA	NA
AXV85012.1\|228333_228555_+	hypothetical protein	A0A2H4JCJ9	uncultured_Caudovirales_phage	5.9e-12	58.9
AXV85013.1\|228554_230102_+\|portal	phage portal protein	A0A1B2LRR5	Wolbachia_phage	7.3e-149	57.1
AXV85014.1\|230111_231362_+\|protease	Clp protease ClpP	K4HZZ6	Acidithiobacillus_phage	2.4e-62	45.0
AXV85015.1\|231371_231749_+\|head	head decoration protein	A0A1B2LRT0	Wolbachia_phage	6.1e-25	49.6
AXV85016.1\|231757_232762_+\|capsid	major capsid protein	A0A1B2LRS0	Wolbachia_phage	9.6e-110	66.6
AXV85017.1\|232764_233067_+	hypothetical protein	NA	NA	NA	NA
AXV85018.1\|233072_233519_+	hypothetical protein	NA	NA	NA	NA
AXV85019.1\|233644_234418_+	hypothetical protein	A0A2D2W2E0	Stenotrophomonas_phage	3.1e-47	43.6
AXV85020.1\|234426_234822_+	hypothetical protein	NA	NA	NA	NA
AXV87853.1\|234818_235040_+	hypothetical protein	NA	NA	NA	NA
AXV85021.1\|235014_235659_+	hypothetical protein	NA	NA	NA	NA
AXV85022.1\|235701_235992_-	DNA-binding protein	NA	NA	NA	NA
AXV85023.1\|236177_237644_-	YOPP/AvrRxv family protein	NA	NA	NA	NA
AXV87854.1\|238253_239381_+	DNA cytosine methyltransferase	Q6J1P4	Burkholderia_virus	9.1e-101	54.6
AXV85024.1\|239446_243550_+	hypothetical protein	A0A0P0ZCJ4	Stx2-converting_phage	7.8e-25	27.8
AXV85025.1\|243555_243951_+	hypothetical protein	A0A0M4UTA7	Ralstonia_phage	1.2e-31	53.0
AXV85026.1\|243951_247542_+	hypothetical protein	A0A0M4U447	Ralstonia_phage	0.0e+00	53.7
AXV85027.1\|247564_248731_+	hypothetical protein	NA	NA	NA	NA
AXV85028.1\|248734_249217_+	hypothetical protein	A0A0M4TU90	Ralstonia_phage	3.1e-13	44.0
AXV85029.1\|249213_249612_+	hypothetical protein	NA	NA	NA	NA
AXV85030.1\|249616_251461_+	hypothetical protein	A0A0M4UKC5	Ralstonia_phage	2.4e-106	46.1
AXV85031.1\|251470_251695_+	hypothetical protein	NA	NA	NA	NA
AXV85032.1\|251762_252053_+	hypothetical protein	K4I011	Acidithiobacillus_phage	4.1e-13	46.4
AXV85033.1\|252049_252526_+	hypothetical protein	K4ICR8	Acidithiobacillus_phage	1.4e-71	85.9
AXV85034.1\|252522_252984_+	glycoside hydrolase	K4I410	Acidithiobacillus_phage	2.3e-66	81.6
AXV85035.1\|253051_253351_+	hypothetical protein	NA	NA	NA	NA
AXV85036.1\|253413_253752_-	DUF1484 domain-containing protein	NA	NA	NA	NA
AXV85037.1\|263188_263923_+	hypothetical protein	NA	NA	NA	NA
AXV85038.1\|264684_267225_+	hemagglutinin	A0A0R6PJK4	Moraxella_phage	7.5e-18	31.2
AXV85039.1\|267236_268988_+	ShlB/FhaC/HecB family hemolysin secretion/activation protein	NA	NA	NA	NA
AXV85040.1\|269000_269846_+	hypothetical protein	NA	NA	NA	NA
AXV87855.1\|270346_271135_+\|transposase	transposase	NA	NA	NA	NA

DBSCAN-SWA_3

859622 : 869753

Hokovirus(14.29%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AXV85539.1\|859622_861584_+	molecular chaperone DnaK	A0A1V0SH73	Hokovirus	8.6e-147	51.0
AXV85540.1\|861718_862861_+	molecular chaperone DnaJ	A0A0P0YMJ9	Yellowstone_lake_phycodnavirus	7.7e-23	32.7
AXV85541.1\|862896_864777_+	aminodeoxychorismate synthase component I	A0A0B5J984	Pandoravirus	1.0e-56	37.3
AXV85542.1\|864732_865419_-	ABC transporter ATP-binding protein	A0A1V0SE00	Indivirus	3.5e-10	22.7
AXV85543.1\|865426_866134_-	ABC transporter permease	NA	NA	NA	NA
AXV85544.1\|866141_866963_-	3-methyl-2-oxobutanoate hydroxymethyltransferase	A0A0N7HVJ8	Ostreococcus_lucimarinus_virus	5.4e-34	31.3
AXV85545.1\|867019_867664_-	deoxynucleoside kinase	A0A249XZR7	Enterococcus_phage	1.1e-05	28.1
AXV85546.1\|867696_868206_-	2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase	NA	NA	NA	NA
AXV85547.1\|868205_869753_-	polynucleotide adenylyltransferase PcnB	H7BUW3	unidentified_phage	2.5e-24	34.5

DBSCAN-SWA_4

1663445 : 1753541

Ralstonia_phage(40.0%)

tRNA,integrase,terminase,capsid,portal,head,plate,tail,holin

attL 1695144:1695161|attR 1729991:1730008

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AXV86233.1\|1663445_1665353_+\|tRNA	threonine--tRNA ligase	A0A2K9L297	Tupanvirus	3.8e-123	37.4
AXV86234.1\|1665381_1665918_+	translation initiation factor IF-3	A0A2L0UZ54	Agrobacterium_phage	2.4e-11	29.9
AXV86235.1\|1666114_1666312_+	50S ribosomal protein L35	NA	NA	NA	NA
AXV86236.1\|1666338_1666695_+	50S ribosomal protein L20	NA	NA	NA	NA
AXV86237.1\|1666808_1667843_+\|tRNA	phenylalanine--tRNA ligase subunit alpha	A0A2H4UW22	Bodo_saltans_virus	9.5e-28	39.8
AXV86238.1\|1667909_1670339_+\|tRNA	phenylalanine--tRNA ligase subunit beta	NA	NA	NA	NA
AXV86239.1\|1670406_1670814_+	integration host factor subunit alpha	A0A1P8CWT5	Bacillus_phage	1.3e-12	42.2
AXV86240.1\|1670838_1671273_+	MerR family transcriptional regulator	NA	NA	NA	NA
AXV86241.1\|1671771_1672944_+	acyltransferase	NA	NA	NA	NA
AXV86242.1\|1673125_1674061_-	aldose epimerase	NA	NA	NA	NA
AXV86243.1\|1674416_1674773_+	hypothetical protein	NA	NA	NA	NA
AXV86244.1\|1674906_1675995_+	DNA polymerase IV	NA	NA	NA	NA
AXV86245.1\|1676196_1677627_+	amino acid permease	NA	NA	NA	NA
AXV86246.1\|1677645_1678449_-	DUF1223 domain-containing protein	NA	NA	NA	NA
AXV86247.1\|1678747_1679035_+	DUF1488 domain-containing protein	NA	NA	NA	NA
AXV86248.1\|1679360_1680167_+	2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase	NA	NA	NA	NA
AXV86249.1\|1680192_1680657_-	homoprotocatechuate degradation operon regulator HpaR	NA	NA	NA	NA
AXV86250.1\|1680673_1681483_-	exodeoxyribonuclease III	NA	NA	NA	NA
AXV86251.1\|1681622_1681841_-	helix-turn-helix domain-containing protein	NA	NA	NA	NA
AXV86252.1\|1681912_1684597_-	bifunctional DNA primase/helicase	A0A077K8T2	Ralstonia_phage	0.0e+00	94.6
AXV86253.1\|1684652_1684976_-	hypothetical protein	NA	NA	NA	NA
AXV86254.1\|1684995_1685199_-	hypothetical protein	A0A077K9T6	Ralstonia_phage	5.0e-18	67.6
AXV86255.1\|1685191_1685434_-	hypothetical protein	A4PE65	Ralstonia_virus	1.5e-29	84.0
AXV86256.1\|1685597_1685837_-	hypothetical protein	NA	NA	NA	NA
AXV86257.1\|1685929_1686178_-	transcriptional regulator	A0A077K829	Ralstonia_phage	2.3e-33	84.1
AXV86258.1\|1686239_1686449_-	hypothetical protein	NA	NA	NA	NA
AXV86259.1\|1686523_1686997_+	XRE family transcriptional regulator	A0A077K9Z1	Ralstonia_phage	4.3e-60	78.7
AXV86260.1\|1687121_1687493_+	hypothetical protein	A0A077K8S5	Ralstonia_phage	4.1e-58	87.0
AXV86261.1\|1687521_1688271_-	site-specific DNA-methyltransferase	A0A1S5NPU0	Burkholderia_phage	6.5e-95	68.1
AXV86262.1\|1688221_1688431_-	Com family DNA-binding transcriptional regulator	A0A1S5NPS9	Burkholderia_phage	2.4e-15	70.4
AXV86263.1\|1688748_1689876_-\|tail	phage tail protein	A4PE54	Ralstonia_virus	2.3e-197	94.4
AXV86264.1\|1689872_1690295_-	oxidoreductase	A4PE53	Ralstonia_virus	2.2e-71	96.4
AXV86265.1\|1690297_1692961_-\|tail	phage tail protein	A4PE52	Ralstonia_virus	0.0e+00	84.4
AXV86266.1\|1692957_1693059_-\|tail	GpE family phage tail protein	A0A077K821	Ralstonia_phage	2.7e-09	93.9
AXV86267.1\|1693055_1693382_-\|tail	phage tail assembly protein	A4PE51	Ralstonia_virus	9.8e-48	95.4
AXV86268.1\|1693457_1693967_-\|tail	phage major tail tube protein	A0A077KER7	Ralstonia_phage	9.2e-77	79.3
AXV86269.1\|1693998_1695174_-\|tail	phage tail protein	A0A077K9Y4	Ralstonia_phage	3.2e-221	96.9
1695144:1695161	attL	NA	NA	NA	NA
AXV86270.1\|1695300_1696053_-\|tail	phage tail protein	A4PE47	Ralstonia_virus	5.7e-107	83.2
AXV86271.1\|1696065_1697733_-\|tail	phage tail protein	A0A077K818	Ralstonia_phage	5.1e-265	83.7
AXV87929.1\|1697739_1698357_-\|tail	phage tail protein I	A0A077KER5	Ralstonia_phage	1.5e-100	96.2
AXV86272.1\|1698349_1699258_-\|plate	baseplate assembly protein	A0A077K9X9	Ralstonia_phage	9.8e-154	94.7
AXV86273.1\|1699260_1699608_-\|plate	baseplate assembly protein	A4PE42	Ralstonia_virus	1.0e-55	94.8
AXV86274.1\|1699604_1700222_-\|plate	phage baseplate assembly protein V	A4PE41	Ralstonia_virus	2.4e-103	89.8
AXV86275.1\|1700351_1701089_+	hypothetical protein	NA	NA	NA	NA
AXV86276.1\|1701093_1701540_-	phage virion morphogenesis protein	A4PE39	Ralstonia_virus	5.4e-65	89.7
AXV86277.1\|1701536_1701971_-\|tail	phage tail protein	A0A077K8R3	Ralstonia_phage	2.3e-68	88.2
AXV86278.1\|1701967_1702468_-	hypothetical protein	A0A077K9R7	Ralstonia_phage	4.2e-74	91.0
AXV86279.1\|1702464_1703268_-	peptidoglycan-binding protein	A4PE36	Ralstonia_virus	1.6e-120	83.8
AXV86280.1\|1703264_1703582_-\|holin	phage holin family protein	A4PE35	Ralstonia_virus	1.2e-42	89.1
AXV86281.1\|1703578_1703986_-	hypothetical protein	A0A077K9X1	Ralstonia_phage	9.8e-21	89.6
AXV86282.1\|1704001_1704205_-\|tail	phage tail protein	A0A077K8R0	Ralstonia_phage	4.2e-25	86.8
AXV86283.1\|1704204_1704684_-\|head	head completion/stabilization protein	A4PE32	Ralstonia_virus	6.4e-72	86.8
AXV86284.1\|1704798_1705503_-\|terminase	terminase	A4PE31	Ralstonia_virus	5.3e-91	76.9
AXV86285.1\|1705499_1706510_-\|capsid	phage major capsid protein, P2 family	A4PE30	Ralstonia_virus	2.0e-163	85.8
AXV86286.1\|1706559_1707402_-\|capsid	capsid scaffolding protein	A4PE29	Ralstonia_virus	1.7e-120	77.9
AXV86287.1\|1707538_1709320_+	oxidoreductase	A0A077K8Q7	Ralstonia_phage	0.0e+00	91.7
AXV86288.1\|1709316_1710402_+\|portal	phage portal protein	A0A077K9Q8	Ralstonia_phage	1.6e-195	91.7
AXV86289.1\|1710418_1711495_-	hypothetical protein	NA	NA	NA	NA
AXV86290.1\|1711873_1712137_-	hypothetical protein	NA	NA	NA	NA
AXV86291.1\|1712145_1713846_-	DUF2875 domain-containing protein	NA	NA	NA	NA
AXV86292.1\|1713839_1716140_-	DUF3274 domain-containing protein	A0A077K801	Ralstonia_phage	4.7e-19	26.4
AXV86293.1\|1716151_1718887_-	type VI secretion system tip protein VgrG	A0A2H4JCD4	uncultured_Caudovirales_phage	1.1e-91	33.4
AXV86294.1\|1719257_1720400_-	amino acid-binding protein	NA	NA	NA	NA
AXV86295.1\|1720528_1720780_-	hypothetical protein	NA	NA	NA	NA
AXV86296.1\|1720893_1721652_-	helix-turn-helix transcriptional regulator	NA	NA	NA	NA
AXV87930.1\|1721954_1722218_-	hypothetical protein	NA	NA	NA	NA
AXV87931.1\|1722276_1722618_-	hypothetical protein	NA	NA	NA	NA
AXV86297.1\|1723050_1723782_-	hypothetical protein	NA	NA	NA	NA
AXV86298.1\|1724387_1725680_-\|integrase	integrase	Q6J1Q2	Burkholderia_virus	5.5e-134	58.7
AXV86299.1\|1726039_1727311_+	cytosine deaminase	NA	NA	NA	NA
AXV86300.1\|1727371_1729492_-	oligopeptidase A	A0A1V0SD92	Indivirus	1.4e-33	21.1
AXV86301.1\|1729611_1730481_-	bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD	A0A249XZQ2	Enterococcus_phage	1.0e-35	38.4
1729991:1730008	attR	NA	NA	NA	NA
AXV86302.1\|1730585_1731218_-	DNA-binding response regulator	NA	NA	NA	NA
AXV87932.1\|1731259_1733833_-	PAS domain-containing sensor histidine kinase	NA	NA	NA	NA
AXV86303.1\|1734148_1736839_+	pyruvate dehydrogenase (acetyl-transferring), homodimeric type	NA	NA	NA	NA
AXV86304.1\|1736996_1738673_+	dihydrolipoyllysine-residue acetyltransferase	NA	NA	NA	NA
AXV86305.1\|1738763_1739339_+	MFS transporter	NA	NA	NA	NA
AXV86306.1\|1739393_1741175_+	dihydrolipoyl dehydrogenase	A0A2K5B2C5	Erysipelothrix_phage	1.5e-36	25.9
AXV86307.1\|1741271_1741829_-	hypothetical protein	NA	NA	NA	NA
AXV86308.1\|1742191_1742773_-	Phasin (PHA-granule associated protein)	NA	NA	NA	NA
AXV86309.1\|1743163_1744138_+	histone deacetylase	NA	NA	NA	NA
AXV86310.1\|1744134_1745052_+	EamA/RhaT family transporter	NA	NA	NA	NA
AXV86311.1\|1745117_1745561_+	thioesterase	NA	NA	NA	NA
AXV86312.1\|1745571_1746279_+	nitrobenzoate reductase	M1I6Q5	Acanthocystis_turfacea_Chlorella_virus	3.0e-25	34.4
AXV86313.1\|1746708_1747884_+	D-alanyl-D-alanine endopeptidase	NA	NA	NA	NA
AXV86314.1\|1747960_1748761_-	IclR family transcriptional regulator	NA	NA	NA	NA
AXV86315.1\|1748999_1749725_+	Fe-S oxidoreductase	NA	NA	NA	NA
AXV86316.1\|1749764_1751174_+	iron-sulfur cluster-binding protein	NA	NA	NA	NA
AXV86317.1\|1751170_1751494_+	hypothetical protein	NA	NA	NA	NA
AXV87933.1\|1751520_1751835_+	NINE protein	NA	NA	NA	NA
AXV86318.1\|1752034_1752808_-	arginyltransferase	NA	NA	NA	NA
AXV86319.1\|1752794_1753541_-\|tRNA	leucyl/phenylalanyl-tRNA--protein transferase	NA	NA	NA	NA

DBSCAN-SWA_5

2664790 : 2673022

Bacillus_virus(16.67%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AXV87046.1\|2664790_2665840_-	methionine ABC transporter ATP-binding protein	G3M9Y6	Bacillus_virus	9.9e-25	33.0
AXV87047.1\|2665996_2666920_-	deacetylase	A0A2K9KZC4	Tupanvirus	6.4e-44	36.1
AXV87048.1\|2667037_2668150_+	lytic murein transglycosylase B	NA	NA	NA	NA
AXV87049.1\|2668229_2669132_-	cysteine synthase CysM	A0A1X9I5K7	Streptococcus_phage	6.3e-52	40.8
AXV87050.1\|2669235_2669553_-	competence protein ComE	NA	NA	NA	NA
AXV87051.1\|2669682_2670678_-	ADP-glyceromanno-heptose 6-epimerase	R9S880	Prochlorococcus_phage	6.3e-29	32.3
AXV87052.1\|2670674_2671622_-	D-glycero-beta-D-manno-heptose-7-phosphate kinase	A0A2H4N7X4	Lake_Baikal_phage	4.5e-16	27.3
AXV87053.1\|2671648_2673022_-	UDP-glucose/GDP-mannose dehydrogenase family protein	A0A127AXI2	Bacillus_phage	1.5e-76	38.9

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage