CRISPRimmunity

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Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Self targeting spacer number	Target MGE spacer number	Prophage number	Anti-CRISPR protein number
CP032297	Rahnella aquatilis strain ZF7 plasmid pRAZF7, complete sequence	0 crisprs	csa3,DEDDh,WYL	0	0	39	0
CP032296	Rahnella aquatilis strain ZF7 chromosome, complete genome	2 crisprs	WYL,DEDDh,DinG,cas3,csa3,RT	0	2	5	0

Results visualization

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CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

0 : 14752

Salmonella_phage(25.0%)

lysis,transposase

The bacterium proteins that are colored denote the protein is present at specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin' and 'tRNA')

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYA09942.1\|586_958_-	hypothetical protein	Q8HAA1	Salmonella_phage	4.1e-42	69.9
AYA09477.1\|1793_2036_+	hypothetical protein	NA	NA	NA	NA
AYA09478.1\|2332_3028_-	helix-turn-helix transcriptional regulator	S5FUZ3	Shigella_phage	7.1e-88	70.0
AYA09479.1\|3125_3371_+	XRE family transcriptional regulator	A0A1C9IHV8	Salmonella_phage	4.4e-16	62.7
AYA09480.1\|3396_3903_+	hypothetical protein	K7PJS5	Enterobacterial_phage	3.8e-46	63.0
AYA09481.1\|4087_4276_+	DUF4222 domain-containing protein	A0A1W6JP38	Morganella_phage	6.1e-10	54.5
AYA09482.1\|4265_5192_+	helix-turn-helix domain-containing protein	U5P0A0	Shigella_phage	5.4e-75	58.1
AYA09483.1\|5173_5878_+	DNA replication protein	A0A193GYX1	Enterobacter_phage	9.9e-45	43.8
AYA09484.1\|5874_6747_+	phage N-6-adenine-methyltransferase	Q8HA94	Salmonella_phage	2.0e-87	54.5
AYA09485.1\|6898_7912_+	DUF968 domain-containing protein	A0A291AWV9	Escherichia_phage	6.5e-74	43.4
AYA09486.1\|7925_8747_+	antitermination protein	F1C595	Cronobacter_phage	5.7e-60	45.2
AYA09487.1\|8978_9194_+	hypothetical protein	NA	NA	NA	NA
AYA09488.1\|9141_9660_+	DUF2335 domain-containing protein	A0A1B0YZW3	Pseudomonas_phage	1.9e-05	28.0
AYA09489.1\|9778_9940_+	transmembrane anchored protein	G9L6D9	Escherichia_phage	4.3e-12	69.8
AYA09490.1\|10075_10648_+	hypothetical protein	NA	NA	NA	NA
AYA09491.1\|10661_10883_-	hypothetical protein	NA	NA	NA	NA
AYA09492.1\|11016_11268_+	hypothetical protein	NA	NA	NA	NA
AYA09493.1\|11350_11683_-	hypothetical protein	NA	NA	NA	NA
AYA09494.1\|11871_12177_+	hypothetical protein	O64361	Escherichia_phage	4.9e-25	55.6
AYA09495.1\|12178_12709_+	lysozyme	Q7Y3V3	Yersinia_phage	1.4e-80	80.0
AYA09496.1\|12701_13205_+\|lysis	lysis protein	S4TP37	Salmonella_phage	3.5e-20	36.4
AYA09497.1\|13201_13495_-	hypothetical protein	NA	NA	NA	NA
AYA09498.1\|13631_14752_-\|transposase	IS3-like element ISRaq1 family transposase	S5WIU1	Leptospira_phage	5.1e-51	42.4

DBSCAN-SWA_2

22587 : 27425

Mollivirus(50.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYA09503.1\|22587_23349_+	2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase KduD	A0A0M4JSW6	Mollivirus	1.3e-05	29.3
AYA09504.1\|23417_24056_+	hypothetical protein	NA	NA	NA	NA
AYA09505.1\|24124_24445_-	cupin domain-containing protein	NA	NA	NA	NA
AYA09506.1\|24448_25630_-	oligogalacturonate lyase	NA	NA	NA	NA
AYA09507.1\|25922_27425_+	proline/glycine betaine transporter ProP	Q6JIH2	Burkholderia_virus	9.1e-56	32.0

DBSCAN-SWA_3

31010 : 32696

Organic_Lake_phycodnavirus(100.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYA09511.1\|31010_32696_-	molecular chaperone HscC	F2Y0P3	Organic_Lake_phycodnavirus	6.4e-82	35.1

DBSCAN-SWA_4

60528 : 63243

Only_Syngen_Nebraska_virus(100.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYA09534.1\|60528_63243_+	magnesium-translocating P-type ATPase	A0A1J0FA34	Only_Syngen_Nebraska_virus	2.2e-39	24.8

DBSCAN-SWA_5

71671 : 72661

Paramecium_bursaria_Chlorella_virus(100.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYA09541.1\|71671_72661_-	acyltransferase	A7ITI3	Paramecium_bursaria_Chlorella_virus	5.9e-11	32.4

DBSCAN-SWA_6

81148 : 84911

uncultured_Caudovirales_phage(75.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYA09547.1\|81148_81469_+	transcriptional regulator	A0A2H4J145	uncultured_Caudovirales_phage	4.1e-22	51.6
AYA09548.1\|81513_82803_+	arsenic transporter	A0A2H4J144	uncultured_Caudovirales_phage	1.2e-165	70.9
AYA09549.1\|82854_83286_+	arsenate reductase (glutaredoxin)	A0A2H4J8T1	uncultured_Caudovirales_phage	4.0e-49	69.3
AYA09550.1\|83357_84911_-	MFS transporter	A0A0P0IY73	Acinetobacter_phage	4.1e-160	57.3

DBSCAN-SWA_7

90426 : 91332

Trichoplusia_ni_ascovirus(100.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYA09555.1\|90426_91332_-	SDR family oxidoreductase	A0A2N9QUX2	Trichoplusia_ni_ascovirus	3.8e-81	56.2

DBSCAN-SWA_8

95534 : 98312

Lactobacillus_phage(100.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYA09560.1\|95534_98312_-	flavocytochrome c	A0A2P0ZL82	Lactobacillus_phage	3.8e-63	30.4

DBSCAN-SWA_9

107986 : 108664

Bacillus_thuringiensis_phage(100.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYA09566.1\|107986_108664_+	SDR family oxidoreductase	Q56AQ6	Bacillus_thuringiensis_phage	1.3e-09	25.6

DBSCAN-SWA_10

112067 : 113801

Tupanvirus(100.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYA09570.1\|112067_113801_+	ABC transporter ATP-binding protein	A0A2K9L0W2	Tupanvirus	4.5e-38	26.0

DBSCAN-SWA_11

118981 : 119761

Cedratvirus(100.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYA09577.1\|118981_119761_-	ATP-binding cassette domain-containing protein	A0A1M7XV31	Cedratvirus	2.8e-16	30.4

DBSCAN-SWA_12

126304 : 134919

Mycobacterium_phage(33.33%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYA09585.1\|126304_127192_-	serine hydrolase	A0A2P0ZZM8	Mycobacterium_phage	1.5e-05	22.8
AYA09586.1\|127346_128105_+	hypothetical protein	NA	NA	NA	NA
AYA09587.1\|128156_130031_-	low affinity potassium transporter Kup	M1IAJ4	Acanthocystis_turfacea_Chlorella_virus	3.0e-64	28.1
AYA09588.1\|130226_130940_-	hypothetical protein	NA	NA	NA	NA
AYA09589.1\|130978_132328_-	hypothetical protein	NA	NA	NA	NA
AYA09590.1\|132348_134919_-	type VI secretion system ATPase TssH	A0A1C3S747	Escherichia_phage	7.0e-88	36.1

DBSCAN-SWA_13

164150 : 165896

Anomala_cuprea_entomopoxvirus(100.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYA09951.1\|164150_165896_+	ABC transporter ATP-binding protein	W6JKT0	Anomala_cuprea_entomopoxvirus	5.0e-21	32.9

DBSCAN-SWA_14

174554 : 181672

Bacillus_virus(20.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYA09625.1\|174554_175334_+	ABC transporter ATP-binding protein	G3M9Y6	Bacillus_virus	2.0e-14	26.7
AYA09626.1\|175330_176050_+	ABC transporter ATP-binding protein	W6JKT0	Anomala_cuprea_entomopoxvirus	4.9e-15	30.2
AYA09627.1\|176083_176923_+	VOC family protein	NA	NA	NA	NA
AYA09628.1\|177122_178136_+	alpha/beta hydrolase	NA	NA	NA	NA
AYA09629.1\|178382_178802_+	translesion error-prone DNA polymerase V autoproteolytic subunit	A0A1W6JNS2	Morganella_phage	1.5e-32	54.5
AYA09630.1\|178804_180067_+	translesion error-prone DNA polymerase V subunit UmuC	I6RSM4	Salmonella_phage	1.9e-171	70.8
AYA09631.1\|180235_181672_-	glycosyl hydrolase family protein	A0A0B5JD41	Pandoravirus	9.1e-37	27.3

DBSCAN-SWA_15

185472 : 187874

Clostridioides_phage(50.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYA09635.1\|185472_186192_-	two-component system response regulator YehT	A0A2R2ZGH8	Clostridioides_phage	1.4e-09	24.5
AYA09636.1\|186185_187874_-	sensor histidine kinase	Q9EYF3	Enterobacteria_phage	3.9e-249	81.5

DBSCAN-SWA_16

199890 : 206849

Klosneuvirus(25.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYA09645.1\|199890_201276_-	diaminobutyrate--2-oxoglutarate transaminase	A0A1V0SKB7	Klosneuvirus	3.5e-25	25.2
AYA09646.1\|201830_202244_-	hypothetical protein	NA	NA	NA	NA
AYA09647.1\|202346_203234_-	class A beta-lactamase	A0A1B0VBP7	Salmonella_phage	1.4e-107	70.9
AYA09648.1\|203359_205024_-	HAMP domain-containing protein	A0A2H4J162	uncultured_Caudovirales_phage	2.4e-17	32.2
AYA09649.1\|205259_206849_-	ABC transporter ATP-binding protein	G9BWD6	Planktothrix_phage	8.0e-18	34.0

DBSCAN-SWA_17

235174 : 237367

Bacillus_phage(100.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYA09674.1\|235174_236641_-	sensor histidine kinase	W8CYF6	Bacillus_phage	4.6e-28	30.6
AYA09675.1\|236644_237367_-	DNA-binding response regulator	W8CYM9	Bacillus_phage	6.2e-34	36.3

DBSCAN-SWA_18

252167 : 255284

Leptospira_phage(100.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYA09692.1\|252167_255284_+	efflux RND transporter permease subunit	S5VTK5	Leptospira_phage	6.5e-40	20.9

DBSCAN-SWA_19

264200 : 268557

Planktothrix_phage(66.67%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYA09699.1\|264200_265610_+	sucrose-6-phosphate hydrolase	F8WPR5	Bacillus_phage	8.1e-22	27.3
AYA09700.1\|265610_266666_+	LacI family DNA-binding transcriptional regulator	NA	NA	NA	NA
AYA09701.1\|266923_267742_+	ABC transporter ATP-binding protein	G9BWD6	Planktothrix_phage	5.0e-08	34.2
AYA09702.1\|267741_268557_+	ABC transporter ATP-binding protein	G9BWD6	Planktothrix_phage	5.5e-15	33.3

DBSCAN-SWA_20

274702 : 276364

Planktothrix_phage(50.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYA09958.1\|274702_275473_-	ATP-binding cassette domain-containing protein	G9BWD6	Planktothrix_phage	9.2e-28	38.7
AYA09959.1\|275497_276364_-	ABC transporter ATP-binding protein	A0A1V0SJ29	Klosneuvirus	1.0e-06	22.8

DBSCAN-SWA_21

281532 : 285390

Pseudoalteromonas_phage(33.33%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYA09709.1\|281532_282195_+	exodeoxyribonuclease X	A0A0H4IT92	Pseudoalteromonas_phage	1.2e-15	30.7
AYA09710.1\|282199_283105_-	LysR family transcriptional regulator	NA	NA	NA	NA
AYA09711.1\|283239_284661_+	MFS transporter	A0A0M3UL24	Mycobacterium_phage	3.7e-22	26.9
AYA09712.1\|284700_285390_+	YafY family transcriptional regulator	A0A1B0RXM1	Streptococcus_phage	7.2e-16	32.3

DBSCAN-SWA_22

294116 : 296270

Salmonella_phage(100.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYA09723.1\|294116_296270_-	TonB-dependent receptor	A0A1B0VCF0	Salmonella_phage	3.1e-129	65.5

DBSCAN-SWA_23

306473 : 307988

Amsacta_moorei_entomopoxvirus(100.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYA09731.1\|306473_307988_+	sugar ABC transporter ATP-binding protein	Q9EMR9	Amsacta_moorei_entomopoxvirus	3.9e-14	26.0

DBSCAN-SWA_24

312938 : 313403

Erwinia_phage(100.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYA09737.1\|312938_313403_-	hypothetical protein	A0A218M4J4	Erwinia_phage	1.1e-07	32.9

DBSCAN-SWA_25

320203 : 320506

Bacillus_phage(100.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYA09745.1\|320203_320506_+	NINE protein	M4ZS56	Bacillus_phage	8.3e-17	70.3

DBSCAN-SWA_26

323753 : 334011

Escherichia_phage(33.33%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYA09750.1\|323753_324374_-	dimethylsulfoxide reductase subunit B	A0A077SL61	Escherichia_phage	1.5e-60	54.8
AYA09751.1\|324370_326746_-	dimethyl sulfoxide reductase subunit A	A0A077SK27	Escherichia_phage	3.1e-143	38.0
AYA09752.1\|327094_328552_+	sugar porter family MFS transporter	O13311	Aichi_virus	2.1e-33	30.4
AYA09753.1\|328727_329402_+	GntR family transcriptional regulator	NA	NA	NA	NA
AYA09754.1\|329557_330682_-	GGDEF domain-containing protein	A0A127AWB9	Bacillus_phage	3.7e-17	35.1
AYA09755.1\|330954_332214_-	aminotransferase class III-fold pyridoxal phosphate-dependent enzyme	A0A1V0SKB7	Klosneuvirus	1.7e-10	29.1
AYA09756.1\|332225_333275_-	aminoglycoside phosphotransferase	NA	NA	NA	NA
AYA09757.1\|333267_334011_-	SDR family oxidoreductase	Q06VL0	Trichoplusia_ni_ascovirus	1.7e-10	28.1

DBSCAN-SWA_27

337692 : 346563

Klosneuvirus(20.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYA09759.1\|337692_338961_-	4-aminobutyrate--2-oxoglutarate transaminase	A0A1V0SKB7	Klosneuvirus	2.4e-25	28.1
AYA09760.1\|339107_340664_+	PLP-dependent aminotransferase family protein	A0A1X9I5H2	Streptococcus_phage	4.6e-18	26.5
AYA09761.1\|340914_342000_+	ABC transporter ATP-binding protein	G3M9Y6	Bacillus_virus	1.3e-27	35.1
AYA09762.1\|343368_343995_+	chromosome partitioning protein ParA	D4HTX7	Vibrio_phage	2.3e-29	37.8
AYA09763.1\|343991_344201_+	chromosome partitioning protein ParB	NA	NA	NA	NA
AYA09764.1\|345576_346563_+	RepB family plasmid replication initiator protein	A0A222YYK1	Escherichia_phage	6.7e-23	29.8

DBSCAN-SWA_28

350528 : 352495

Bacillus_virus(50.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYA09768.1\|350528_351533_+	ABC transporter ATP-binding protein	G3M9Y6	Bacillus_virus	1.9e-12	29.7
AYA09769.1\|351529_352495_+	ATP-binding cassette domain-containing protein	G9BWD6	Planktothrix_phage	1.8e-17	29.0

DBSCAN-SWA_29

356199 : 357399

Oenococcus_phage(100.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYA09773.1\|356199_357399_+	starvation-sensing protein RspA	Q6A202	Oenococcus_phage	4.3e-40	29.6

DBSCAN-SWA_30

363141 : 365241

Ostreococcus_lucimarinus_virus(100.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYA09776.1\|363141_365241_-	lipase family protein	G9E505	Ostreococcus_lucimarinus_virus	7.1e-06	27.1

DBSCAN-SWA_31

375679 : 377479

Planktothrix_phage(100.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYA09972.1\|375679_377479_+	ABC transporter ATP-binding protein	G9BWD6	Planktothrix_phage	5.0e-16	31.7

DBSCAN-SWA_32

384171 : 384888

Planktothrix_phage(100.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYA09973.1\|384171_384888_+	ABC transporter ATP-binding protein	G9BWD6	Planktothrix_phage	1.6e-37	41.2

DBSCAN-SWA_33

392451 : 393768

Pandoravirus(100.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYA09797.1\|392451_393768_-	cytochrome P450	S4VXU7	Pandoravirus	3.5e-11	32.0

DBSCAN-SWA_34

400668 : 408801

Tupanvirus(66.67%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYA09806.1\|400668_402183_-	AMP-dependent synthetase	A0A2K9KZV5	Tupanvirus	8.1e-28	24.4
AYA09807.1\|402151_403705_-	AMP-dependent synthetase	NA	NA	NA	NA
AYA09808.1\|403705_403960_-	phosphopantetheine-containing protein	NA	NA	NA	NA
AYA09809.1\|403970_405551_-	long-chain fatty acid--CoA ligase	A0A2K9KZV5	Tupanvirus	2.1e-26	23.4
AYA09810.1\|405547_406570_-	3-oxoacyl-ACP synthase	NA	NA	NA	NA
AYA09811.1\|406572_407484_-	hypothetical protein	NA	NA	NA	NA
AYA09812.1\|407493_408801_-	type III PLP-dependent enzyme	A0A1V0SKK0	Klosneuvirus	2.7e-11	22.7

DBSCAN-SWA_35

441502 : 442039

Leuconostoc_phage(100.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYA09974.1\|441502_442039_+	adenylate kinase	A0A097BYE2	Leuconostoc_phage	8.1e-15	39.8

DBSCAN-SWA_36

461706 : 462483

Mollivirus(100.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYA09873.1\|461706_462483_-	SDR family oxidoreductase	A0A0M4JSW6	Mollivirus	1.1e-09	27.2

DBSCAN-SWA_37

465581 : 466322

Trichoplusia_ni_ascovirus(100.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYA09877.1\|465581_466322_-	SDR family oxidoreductase	Q06VL0	Trichoplusia_ni_ascovirus	2.8e-13	30.0

DBSCAN-SWA_38

473859 : 478645

Aeromonas_phage(33.33%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYA09885.1\|473859_474615_-	LuxR family transcriptional regulator	A0A1I9KF49	Aeromonas_phage	5.1e-07	26.3
AYA09886.1\|474842_475781_+	ABC transporter permease	NA	NA	NA	NA
AYA09887.1\|475777_476638_+	ABC transporter permease	NA	NA	NA	NA
AYA09888.1\|476634_477612_+	ABC transporter ATP-binding protein	G3M9Y6	Bacillus_virus	4.2e-09	26.8
AYA09889.1\|477595_478645_+	ABC transporter ATP-binding protein	Q6GZ03	Mycoplasma_phage	6.1e-06	31.8

DBSCAN-SWA_39

487798 : 541608

uncultured_Caudovirales_phage(25.0%)

integrase,plate

attL 483526:483540|attR 541215:541229

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
483526:483540	attL	NA	NA	NA	NA
AYA09898.1\|487798_488236_-\|plate	type VI secretion system baseplate subunit TssE	NA	NA	NA	NA
AYA09976.1\|488228_488726_-	type VI secretion system lipoprotein TssJ	NA	NA	NA	NA
AYA09899.1\|488745_489843_-\|plate	type VI secretion system baseplate subunit TssG	NA	NA	NA	NA
AYA09900.1\|489806_491570_-\|plate	type VI secretion system baseplate subunit TssF	NA	NA	NA	NA
AYA09901.1\|491879_492365_-	hypothetical protein	NA	NA	NA	NA
AYA09902.1\|492561_493212_-	DUF2931 family protein	NA	NA	NA	NA
AYA09903.1\|493251_493899_-	DUF2931 family protein	NA	NA	NA	NA
AYA09904.1\|493938_494586_-	DUF2931 family protein	NA	NA	NA	NA
AYA09905.1\|494582_496166_-	DUF2235 domain-containing protein	NA	NA	NA	NA
AYA09906.1\|496195_498703_-	type VI secretion system tip protein VgrG	A0A2H4JCD4	uncultured_Caudovirales_phage	1.7e-14	29.9
AYA09907.1\|498725_500330_-	type VI secretion system protein TssA	NA	NA	NA	NA
AYA09908.1\|500329_503746_-	type VI secretion protein VasK	NA	NA	NA	NA
AYA09909.1\|503729_504893_-	type VI secretion protein	NA	NA	NA	NA
AYA09910.1\|504896_505163_-	PAAR domain-containing protein	NA	NA	NA	NA
AYA09911.1\|506375_507134_-	hypothetical protein	NA	NA	NA	NA
AYA09912.1\|507165_507939_-	hypothetical protein	NA	NA	NA	NA
AYA09913.1\|507935_509885_-	lipase family protein	G9E505	Ostreococcus_lucimarinus_virus	7.8e-07	29.9
AYA09914.1\|509931_510360_-	hypothetical protein	NA	NA	NA	NA
AYA09915.1\|510356_510641_-	hypothetical protein	NA	NA	NA	NA
AYA09916.1\|510692_513239_-	type VI secretion system tip protein VgrG	A0A2H4JCD4	uncultured_Caudovirales_phage	5.0e-14	30.7
AYA09917.1\|513312_513657_-	hypothetical protein	NA	NA	NA	NA
AYA09918.1\|514650_514830_-	hypothetical protein	NA	NA	NA	NA
AYA09919.1\|514839_517497_-	type VI secretion system ATPase TssH	A0A1S6UBG5	Serratia_phage	1.2e-90	32.8
AYA09920.1\|517659_518151_-	Hcp family type VI secretion system effector	NA	NA	NA	NA
AYA09921.1\|518183_519878_-	OmpA family protein	NA	NA	NA	NA
AYA09922.1\|519881_520535_-	DotU family type IV/VI secretion system protein	NA	NA	NA	NA
AYA09923.1\|520531_521869_-\|plate	type VI secretion system baseplate subunit TssK	NA	NA	NA	NA
AYA09924.1\|521880_523428_-	type VI secretion system contractile sheath large subunit	NA	NA	NA	NA
AYA09925.1\|523459_523954_-	type VI secretion system contractile sheath small subunit	NA	NA	NA	NA
AYA09926.1\|524800_526348_+	HAMP domain-containing protein	A0A2H4J162	uncultured_Caudovirales_phage	3.0e-09	54.5
AYA09927.1\|528362_529487_+\|integrase	integrase	A0A1P8DTG6	Proteus_phage	2.1e-28	27.1
AYA09928.1\|529633_531775_+	5-histidylcysteine sulfoxide synthase	NA	NA	NA	NA
AYA09929.1\|532073_532241_-	DUF4223 domain-containing protein	NA	NA	NA	NA
AYA09930.1\|532578_534177_+	tannase/feruloyl esterase family alpha/beta hydrolase	NA	NA	NA	NA
AYA09931.1\|534597_535464_+	helix-turn-helix domain-containing protein	NA	NA	NA	NA
AYA09932.1\|535528_536308_-	(S)-acetoin forming diacetyl reductase	Q06VL0	Trichoplusia_ni_ascovirus	1.9e-17	31.5
AYA09933.1\|536537_536951_+	hypothetical protein	NA	NA	NA	NA
AYA09934.1\|537109_537298_+	hypothetical protein	NA	NA	NA	NA
AYA09935.1\|537907_538579_+	porin	Q1MVN1	Enterobacteria_phage	1.4e-64	58.2
AYA09936.1\|538653_539778_-\|integrase	integrase	Q77Z04	Phage_21	2.3e-104	51.7
AYA09937.1\|539761_540004_-	excisionase	NA	NA	NA	NA
AYA09938.1\|540337_540826_-	SAM-dependent methyltransferase	H9C170	Pectobacterium_phage	7.5e-68	74.8
AYA09939.1\|540818_541115_-	DUF4406 domain-containing protein	A0A222YYT7	Escherichia_phage	9.9e-23	60.0
AYA09940.1\|541116_541608_-	hypothetical protein	Q7Y3W7	Yersinia_phage	2.0e-36	44.7
541215:541229	attR	NA	NA	NA	NA

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

2. CP032296

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Crispr_ID: CP032296_1

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

CP032296_1

1909251-1909518

Orphan

I-F

Consensus_repeat	Method
GTTCACTGCCGCACAGGCAGCTTAGAAA	PILER-CR
GTTCACTGCCGGACAGGCAGCTTAGAAA	CRISPRCasFinder
GTTCACTGCCGNACAGGCAGCTTAGAAA	CRT

4 spacers

The CRISPR arrays of CP032296_1

>merge|CP032296|1|1909251-1909518|PILER-CR,CRISPRCasFinder,CRT
GTTCACTGCCGGACAGGCAGCTTAGAAAATACGATCGCAACGTCTGTCATAGCGGTGTGCGTTCACTGCCGGACAGGCAGCTTAGAAAGCTGAGTGCACGGTGTTGATCGCATTCTCAATGTTCACTGCCGCACAGGCAGCTTAGAAATCGGATAGCAATCTTGTTCAATAGCTGACTCTGTTCACTGCCGCACAGGCAGCTTAGAAAGTTACTGATGTCGTAATCAATAATCGACTGAAGTTCACTGCCGCACAGGCAGCTTAGAAA

>CP032296|1|1|1909251-1909518|PILER-CR
GTTCACTGCCGGACAGGCAGCTTAGAAA	ATACGATCGCAACGTCTGTCATAGCGGTGTGC
GTTCACTGCCGGACAGGCAGCTTAGAAA	GCTGAGTGCACGGTGTTGATCGCATTCTCAAT
GTTCACTGCCGCACAGGCAGCTTAGAAA	TCGGATAGCAATCTTGTTCAATAGCTGACTCT
GTTCACTGCCGCACAGGCAGCTTAGAAA	GTTACTGATGTCGTAATCAATAATCGACTGAA
GTTCACTGCCGCACAGGCAGCTTAGAAA

>CP032296|1|1|1909251-1909518|CRISPRCasFinder
GTTCACTGCCGGACAGGCAGCTTAGAAA	ATACGATCGCAACGTCTGTCATAGCGGTGTGC
GTTCACTGCCGGACAGGCAGCTTAGAAA	GCTGAGTGCACGGTGTTGATCGCATTCTCAAT
GTTCACTGCCGCACAGGCAGCTTAGAAA	TCGGATAGCAATCTTGTTCAATAGCTGACTCT
GTTCACTGCCGCACAGGCAGCTTAGAAA	GTTACTGATGTCGTAATCAATAATCGACTGAA
GTTCACTGCCGCACAGGCAGCTTAGAAA

>CP032296|1|1|1909251-1909518|CRT
GTTCACTGCCGGACAGGCAGCTTAGAAA	ATACGATCGCAACGTCTGTCATAGCGGTGTGC
GTTCACTGCCGGACAGGCAGCTTAGAAA	GCTGAGTGCACGGTGTTGATCGCATTCTCAAT
GTTCACTGCCGCACAGGCAGCTTAGAAA	TCGGATAGCAATCTTGTTCAATAGCTGACTCT
GTTCACTGCCGCACAGGCAGCTTAGAAA	GTTACTGATGTCGTAATCAATAATCGACTGAA
GTTCACTGCCGCACAGGCAGCTTAGAAA

Protein	Signature genes	Signature genes Name	Protein_function
CP032296.1\|AYA06651.1\|1912367_1912664_+\|hypothetical-protein	unknown	unknown	unknown
CP032296.1\|AYA06658.1\|1919279_1919924_+\|protein-phosphatase-CheZ	unknown	unknown	gnl\|CDD\|236868
CP032296.1\|AYA06652.1\|1912672_1913266_+\|hypothetical-protein	unknown	unknown	unknown
CP032296.1\|AYA06648.1\|1908266_1908764_+\|chemotaxis-protein-CheW	unknown	unknown	gnl\|CDD\|182587
CP032296.1\|AYA06646.1\|1905007_1906111_+\|motility-protein-MotB	unknown	unknown	gnl\|CDD\|236365
CP032296.1\|AYA06655.1\|1916911_1917784_+\|protein-glutamate-O-methyltransferase-CheR	unknown	unknown	gnl\|CDD\|236725
CP032296.1\|AYA06642.1\|1900901_1901726_+\|NAD-dependent-epimerase/dehydratase-family-protein	unknown	unknown	gnl\|CDD\|187576
CP032296.1\|AYA06639.1\|1897026_1898160_-\|histidinol-phosphate-transaminase	unknown	unknown	gnl\|CDD\|234972
CP032296.1\|AYA06647.1\|1906141_1908184_+\|chemotaxis-protein-CheA	unknown	unknown	gnl\|CDD\|236712
CP032296.1\|AYA06656.1\|1917783_1918833_+\|chemotaxis-response-regulator-protein-glutamate-methylesterase	unknown	unknown	gnl\|CDD\|234828
CP032296.1\|AYA06650.1\|1910693_1912169_+\|MFS-transporter	unknown	unknown	gnl\|CDD\|129794
CP032296.1\|AYA06657.1\|1918879_1919269_+\|chemotaxis-protein-CheY	unknown	unknown	gnl\|CDD\|170568
CP032296.1\|AYA06654.1\|1915178_1916849_+\|HAMP-domain-containing-protein	unknown	unknown	gnl\|CDD\|185001
CP032296.1\|AYA06644.1\|1903335_1903944_+\|flagellar-transcriptional-regulator-FlhC	unknown	unknown	gnl\|CDD\|237181
CP032296.1\|AYA06645.1\|1904120_1905011_+\|flagellar-motor-stator-protein-MotA	unknown	unknown	gnl\|CDD\|181655
CP032296.1\|AYA06643.1\|1902988_1903333_+\|flagellar-transcriptional-regulator-FlhD	unknown	unknown	gnl\|CDD\|179497
CP032296.1\|AYA06640.1\|1898159_1899473_-\|histidinol-dehydrogenase	unknown	unknown	gnl\|CDD\|234853
CP032296.1\|AYA06641.1\|1899476_1900376_-\|ATP-phosphoribosyltransferase	unknown	unknown	gnl\|CDD\|234781
CP032296.1\|AYA06649.1\|1909565_1910579_-\|transcriptional-regulator-FtrA	unknown	unknown	gnl\|CDD\|181818
CP032296.1\|AYA06653.1\|1913362_1915057_+\|HAMP-domain-containing-protein	unknown	unknown	gnl\|CDD\|185001

Protein	Function_ID	Function_description	E-value
CP032296.1\|AYA06658.1\|1919279_1919924_+\|protein-phosphatase-CheZ	gnl\|CDD\|236868	PRK11166, PRK11166, chemotaxis regulator CheZ; Provisional.	7.60403e-139
CP032296.1\|AYA06648.1\|1908266_1908764_+\|chemotaxis-protein-CheW	gnl\|CDD\|182587	PRK10612, PRK10612, chemotaxis protein CheW.	9.24838e-106
CP032296.1\|AYA06646.1\|1905007_1906111_+\|motility-protein-MotB	gnl\|CDD\|236365	PRK09041, motB, motility protein MotB.	1.1216e-163
CP032296.1\|AYA06655.1\|1916911_1917784_+\|protein-glutamate-O-methyltransferase-CheR	gnl\|CDD\|236725	PRK10611, PRK10611, protein-glutamate O-methyltransferase CheR.	0
CP032296.1\|AYA06642.1\|1900901_1901726_+\|NAD-dependent-epimerase/dehydratase-family-protein	gnl\|CDD\|187576	cd05266, SDR_a4, atypical (a) SDRs, subgroup 4. Atypical SDRs in this subgroup are poorly defined, one member is identified as a putative NAD-dependent epimerase/dehydratase. Atypical SDRs are distinct from classical SDRs. Members of this subgroup have a glycine-rich NAD(P)-binding motif that is related to, but is different from, the archetypical SDRs, GXGXXG. This subgroup also lacks most of the characteristic active site residues of the SDRs; however, the upstream Ser is present at the usual place, and some potential catalytic residues are present in place of the usual YXXXK active site motif. Atypical SDRs generally lack the catalytic residues characteristic of the SDRs, and their glycine-rich NAD(P)-binding motif is often different from the forms normally seen in classical or extended SDRs. Atypical SDRs include biliverdin IX beta reductase (BVR-B,aka flavin reductase), NMRa (a negative transcriptional regulator of various fungi), progesterone 5-beta-reductase like proteins, phenylcoumaran benzylic ether and pinoresinol-lariciresinol reductases, phenylpropene synthases, eugenol synthase, triphenylmethane reductase, isoflavone reductases, and others. SDRs are a functionally diverse family of oxidoreductases that have a single domain with a structurally conserved Rossmann fold, an NAD(P)(H)-binding region, and a structurally diverse C-terminal region. Sequence identity between different SDR enzymes is typically in the 15-30% range; they catalyze a wide range of activities including the metabolism of steroids, cofactors, carbohydrates, lipids, aromatic compounds, and amino acids, and act in redox sensing. Classical SDRs have an TGXXX[AG]XG cofactor binding motif and a YXXXK active site motif, with the Tyr residue of the active site motif serving as a critical catalytic residue (Tyr-151, human 15-hydroxyprostaglandin dehydrogenase numbering). In addition to the Tyr and Lys, there is often an upstream Ser and/or an Asn, contributing to the active site; while substrate binding is in the C-terminal region, which determines specificity. The standard reaction mechanism is a 4-pro-S hydride transfer and proton relay involving the conserved Tyr and Lys, a water molecule stabilized by Asn, and nicotinamide. In addition to the Rossmann fold core region typical of all SDRs, extended SDRs have a less conserved C-terminal extension of approximately 100 amino acids, and typically have a TGXXGXXG cofactor binding motif. Complex (multidomain) SDRs such as ketoreductase domains of fatty acid synthase have a GGXGXXG NAD(P)-binding motif and an altered active site motif (YXXXN). Fungal type ketoacyl reductases have a TGXXXGX(1-2)G NAD(P)-binding motif.	6.10457e-73
CP032296.1\|AYA06639.1\|1897026_1898160_-\|histidinol-phosphate-transaminase	gnl\|CDD\|234972	PRK01688, PRK01688, histidinol-phosphate aminotransferase; Provisional.	0
CP032296.1\|AYA06647.1\|1906141_1908184_+\|chemotaxis-protein-CheA	gnl\|CDD\|236712	PRK10547, PRK10547, chemotaxis protein CheA; Provisional.	0
CP032296.1\|AYA06656.1\|1917783_1918833_+\|chemotaxis-response-regulator-protein-glutamate-methylesterase	gnl\|CDD\|234828	PRK00742, PRK00742, chemotaxis-specific protein-glutamate methyltransferase CheB.	0
CP032296.1\|AYA06650.1\|1910693_1912169_+\|MFS-transporter	gnl\|CDD\|129794	TIGR00711, Uncharacterized_MFS-type_transporter_YhcA, drug resistance transporter, EmrB/QacA subfamily. This subfamily of drug efflux proteins, a part of the major faciliator family, is predicted to have 14 potential membrane-spanning regions. Members with known activities include EmrB (multiple drug resistance efflux pump) in E. coli, FarB (antibacterial fatty acid resistance) in Neisseria gonorrhoeae, TcmA (tetracenomycin C resistance) in Streptomyces glaucescens, etc. In most cases, the efflux pump is described as having a second component encoded in the same operon, such as EmrA of E. coli. [Cellular processes, Toxin production and resistance, Transport and binding proteins, Other].	9.82012e-52
CP032296.1\|AYA06657.1\|1918879_1919269_+\|chemotaxis-protein-CheY	gnl\|CDD\|170568	PRK10610, PRK10610, chemotaxis protein CheY.	5.35691e-83
CP032296.1\|AYA06654.1\|1915178_1916849_+\|HAMP-domain-containing-protein	gnl\|CDD\|185001	PRK15041, PRK15041, methyl-accepting chemotaxis protein.	1.03282e-170
CP032296.1\|AYA06644.1\|1903335_1903944_+\|flagellar-transcriptional-regulator-FlhC	gnl\|CDD\|237181	PRK12722, PRK12722, flagellar transcriptional regulator FlhC.	1.43807e-141
CP032296.1\|AYA06645.1\|1904120_1905011_+\|flagellar-motor-stator-protein-MotA	gnl\|CDD\|181655	PRK09110, PRK09110, flagellar motor stator protein MotA.	0
CP032296.1\|AYA06643.1\|1902988_1903333_+\|flagellar-transcriptional-regulator-FlhD	gnl\|CDD\|179497	PRK02909, PRK02909, flagellar transcriptional regulator FlhD.	6.24818e-56
CP032296.1\|AYA06640.1\|1898159_1899473_-\|histidinol-dehydrogenase	gnl\|CDD\|234853	PRK00877, hisD, bifunctional histidinal dehydrogenase/ histidinol dehydrogenase; Reviewed.	0
CP032296.1\|AYA06641.1\|1899476_1900376_-\|ATP-phosphoribosyltransferase	gnl\|CDD\|234781	PRK00489, hisG, ATP phosphoribosyltransferase; Reviewed.	5.18115e-159
CP032296.1\|AYA06649.1\|1909565_1910579_-\|transcriptional-regulator-FtrA	gnl\|CDD\|181818	PRK09393, ftrA, transcriptional activator FtrA; Provisional.	8.45281e-160
CP032296.1\|AYA06653.1\|1913362_1915057_+\|HAMP-domain-containing-protein	gnl\|CDD\|185001	PRK15041, PRK15041, methyl-accepting chemotaxis protein.	0

>CP032296.1|AYA06648.1|1908266_1908764_+|chemotaxis-protein-CheW
MAGLATVAKIAGETVGQEFLIFTLGDEEYGIDILKVQEIRGYDQVTRIANTPAFIKGVTNLRGVIVPILDLRVKFAQEDVIYNENTVVIVLNFENRVVGIVVDGVSDVLSLSQDQIRPAPEFAVTMSTEYLTGLGSLGDRMLILVDIEKLLSSEEMALVDSVTKI
>CP032296.1|AYA06647.1|1906141_1908184_+|chemotaxis-protein-CheA
MSMDITDFYQTFFDEADELLADMEQHLLLLDPQAPDIEQLNAIFRAAHSIKGGAATFGFTVLQETTHLLENLLDGARRAEMSLSTDIINLFLETKDIMQEQLDAYKSSRDPDAESFEYICAALRQLALEAQQAREPVPDLHVVEDQTAAQSVATAPVAPAAGKGDLRVRLSGLKTQEIPVMLEELGNLGEIKDPVQTDDSVEATLVTTVSEDDIIAVLCFVLEPEQISFTHAPAAVASEAVAVAASAPVVAEVAAAPAVVARQAPVLTAVPNSEPAKARAPKAPESTSIRVAVEKVDQLINLVGELVITQSMLAQRSDLLDPVVHSDLLNSMGQLERNARDLQESVMSIRMMPMEFVFSRFPRLVRDLASKLNKQIELTLLGSSTELDKSLIERIIDPLTHLVRNSLDHGVETPDVRIANGKEPTGNLTLSAEHQGGNICIEVIDDGAGLNRERILAKAASQGMNVHDNMSDEEVGMLIFAAGFSTAEKVTDVSGRGVGMDVVKRNIQEMGGHVEIHSQQGKGTTIRILLPLTLAILDGMSVKVGKEVFILPLNAVMESLQPLSEDLHPMAGGERVLQVRGEYLPLVELYNVFDVEDAKTEATQGIVVILQSAGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAATILGDGSVALIVDVSALQSLNRDKLATGSTAA
>CP032296.1|AYA06646.1|1905007_1906111_+|motility-protein-MotB
MKHQSHPIVVVRKKKNKHGGGHHGGSWKIAYADFMTAMMAFFLVMWLISISSPKELVQIAEYFRTPLAVAITGGPRSSDATSPIPGGGEDPTVQDGEVHKMEQSQGASKRESDDLQRLQQKLEDMLASDPRLKDLRPHLMINLTEQGLRIQIIDSKNRPMFQLGSAIVEPYMRDILRAIAPVLDGFPNKISLAGHTDDMPYATGERGYSNWELSTERANASRREMMSGGLSEGKVLRVMGMGSSMQLKNHPAGDAINRRISILVLNKHAEEAIERENSEGEAITISDAGELQRQVQGTQKVPALAPKMSSAPAAPALAPQANPAPATPEKAPSIPGIETQTPVTPSAGAQPATAAPAAAAGTAPATH
>CP032296.1|AYA06645.1|1904120_1905011_+|flagellar-motor-stator-protein-MotA
MLVIIGYIVVLGSVFGGFMLVGGELGALYQPAELLIIAGAGIGAFFVGNNGKAIKSTMRALPQLFRASKYNKALYMDLMALLYRVLAKSRQQGMLSLENDIDNPAESDIFANYPRILADKQLVEYLTDYLRLMVSGNMNAFEIEALMDEEIETYEHESEVPANSLALVGDSLPAFGIVAAVMGIVHTLAAADRPAAELGALIAHAMVGTFLGILLAYGFVSPLASVLRQKSAENAKMMQCIKVTLLSSINGYAPQIAVEFGRKTLYTSERPSFIELEEHVRQVKSPGQKQTEEEKV
>CP032296.1|AYA06644.1|1903335_1903944_+|flagellar-transcriptional-regulator-FlhC
MSGKDASTKPAEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLYKELRGSPPPKGMLPFSTDWFMTWEQNIHSSMFYNAYSFLLKSGHCNGVEAVIKAYRLYLEQCPEQPGEAPILALTRAWTLVRFVDSGMLQLSACNCCDGSFITHAHQPRHGFVCSLCQPPSRAVKKRKLSPQVADITSQELDEQVKHAV
>CP032296.1|AYA06643.1|1902988_1903333_+|flagellar-transcriptional-regulator-FlhD
MSELLKHIYDINLSYLLLAQRLINDEKASAMFRLGIDDVMADTLRQLTLPQMVKLAETNQLLCQFRFDDSKTIERLTQESRVDDLQQIHTGILLSSHLLKGLAAKESGAMKKRA
>CP032296.1|AYA06642.1|1900901_1901726_+|NAD-dependent-epimerase/dehydratase-family-protein
MKKVSIIGLGWLGMPLAMALASKGYQVVGSKTTPDGVDAARAVGIECFPLVFTPQMECVAEDLDQLLNVDALIITLPARRTVDGSELYFQAVQLLTDSALAHNVKRIIFTSSTSVYGEGEGVVREGSPLEPVTTSGKVLKELENWLHGLPNTSVDVLRLAGLVGGDRHPGRFMAGKTQVKGGQHGVNLVHQDDVIAAIALLLSLPKGGHTYNLCAPAHPAKAEFYPALARQMGLEPPTFAEQASAQKGKIVDGERICKELGFEYQHPDPRTMPF
>CP032296.1|AYA06641.1|1899476_1900376_-|ATP-phosphoribosyltransferase
MLDKTRLRIAMQKSGRLSDESRELLARCGIKINLQEQRLIAFAENMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLSRRAQGEDPRYFTLRRLDFGGCRLSLATPLDTEYTGPQSLQNARIATSYPHLLKQYLDKKGVDFKSCLLNGSVEVAPRAGLSDAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMPQEKQLLIDKMMTRIQGVIQARESKYIMLHAPSERLDEIVALLPGAERPTILPLAGDQQRVAMHMVSSETLFWETMEKLKALGASSILVLPIEKMME
>CP032296.1|AYA06640.1|1898159_1899473_-|histidinol-dehydrogenase
MKPDAFTTPIDWQQCDEQQRRELLTRPAISASDRITESVSEILRRVRSEGDSALREFCARFDKTEVRELRVTAQQIGAAAARLGEDVKQAMAHAVRNIDTFHQAQKLAVVDIETQPGVRCQQLTRPIQSVGLYIPGGSAPLVSTVLMLATPARIAGCQKVVLCSPPPIADEILYAAQLCGVEEVYQLGGAQAIAALAFGTETVPRVAKIFGPGNAYVTEAKRQVSQSLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDLKMAQAVGEAVERQLQTLSRSEIARQALASSRLIVAKDIAQCIEISNAYGPEHLILQTREPEQLVDSITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTVQQLTPEGLLGLANTIETLAQAEQLTAHKNAVTLRVNALKEQR
>CP032296.1|AYA06639.1|1897026_1898160_-|histidinol-phosphate-transaminase
MSNENTLSVQALARKNVQELTPYQSARRLGGNGDVWLNANEYAIAPQFELTQQTFNRYPECQPALVINRYAAYAGVDPEQVLVSRGADEGIELLIRAFCEPGKDAILYCPPTYGMYSVSAETIGVEGRTVAAKADWSLDIPAIEAALDGVKLVYVCSPNNPTGNIIDPQEIRAVLEMTRGRAIVAVDEAYIEFCPQATVSGWLQEYPHLAILRTLSKAFALAGLRCGFTLANSDLIGLLMKVIAPYPLSTPVADIAAQALSPEGLAIMRQRVTEVTENRAWLREELEKCACVEEVFASDTNYVLVRFYASSAVFKNLWDQGIILRDQNKQPGLSGCLRITIGTREELQRVITALQPLPGATASTLNDNQNSPRKEHM
>CP032296.1|AYA06649.1|1909565_1910579_-|transcriptional-regulator-FtrA
MQKVDLPLMSSSRHPAAPNRRLVLLAYECLCTFEFGIAVEAFGRADELTGEPLYELAVASVEEGTFGAQGGVRLVVDGGLELLEDAGTIVIPGWRSIHEPASERLIAALQKAHHNGTRIVSICAGSFVLAAAGLLDGKRATAHWNSTDVLAQKFPDVQVEHGMIYVDEGSIITSAGGAAGVDLCLHLIRRDYGIEVANRTARRMVTPPLREGNQAQLIQQPVPRHQTKTLAPLLDDLRGHLNTPLVIDQLARQAGMSRRTFLRRFHDATGTTPGEWMLTVRLEKACALLENGTLSIDRVAEQAGFGSPETLRHHFRQRLGMSPTQWRKGFVERDLVG
>CP032296.1|AYA06650.1|1910693_1912169_+|MFS-transporter
MKNTLALMAVCLAALMSGLEISSVPVLSPVLEQELHASFRELQWIMNAYTLACTTVLMATGTLADRYGRKRVFIISITAFGLTSLMCGLAGSAQWLIAGRFLQGLSGGAMLTCLIAILSSQFPQGKARSQAFAAWGITFGFGLGFGPIIGGTLVAWLSWRWVFLIHVVIAVLTLILALKNVVESRDGQAKKLDLGGLITLSVTVLGATFLITQGSSLGWSSVTAGAVLLATAVSAVMFIRIEKRHPHPMFDFSVFRVRAFSGALMGSVGMNFSFWPLMIYLPVYYQGGLGYGSVATGMALLAYTLPTLLMPPLGEKLTTRFGASRLIPLGLFTIGAGFMLMQIAVVHHLSLLPGALLAGIALGLTNTPVTNTTTGSVAANRAGMASGIDISARLITLAINIALMGNLLVAGIAASLKKVMPAAGELSSLAERIAGGSAIEPLQSGQIAQALTDGFAGILLYGAAGVWVLALLSYLLFNGRGRRKTVSCEPS
>CP032296.1|AYA06651.1|1912367_1912664_+|hypothetical-protein
MSFREIKWKVIECLKNGNIEFEARRGIQLKNLLSTGDISPFEVAALIGRAPGDHYQVRPYHFDSSIDVHIITVSSAGVPWYIKWYFTEPTSVFISVHH
>CP032296.1|AYA06652.1|1912672_1913266_+|hypothetical-protein
MIIYKVGDEKKAMCGVCEAFRIVSYQLRDVPFDDGSGMVKNIIAGVCKTCDSVAVIPFQSVPAIKKQLQIQRKAVETRVPAHMIDLLNMASAELGATPDFVPLLLKYYIHQLASNPEAALRLVTLLTSELATGLANKRLSLKGREIGNDIEKLKSLSQIDNTTELLKGVVLMIHHDLLVNPDAHRIAVLKSFVATVA
>CP032296.1|AYA06653.1|1913362_1915057_+|HAMP-domain-containing-protein
MFNRMKVVTGIVILLVVFGALQLVSGGLFFKSLKTDKDNFTTSQQIRLQQAEFNASWIYLLQTRNTLNRAGIRFMLDANQMGSGATVKELIDTATKDLQIADQHFASYQKIPADASQNPQFAADAKKQYQVLHDALSELIELIGLGRINDFFEQPTQQYQDNFEKAFVTYIDQNDRLYNEAVKVSENSFTHAIWMLGTVLIVLAIFILLAWTGVQRILIHPLKNIVDTIRKIATGDLTHEIVVKSRNEIGQLADSLKYMQDELIRTVSGVRHGADAIYSGASEISAGNSDLSSRTEQQASALEETAASMEQLTATVKQNAENARQASQLALSASETAQKGGKVVANVVDTMNDIASSSKKITDITGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVRTLAQRSAQAAKEIKGLIDDSVSRINTGSVLAESAGETMHDMVNAVTRVTDIMGEITSASDEQSRGIEQVAQAITEMDRVTQQNASLVEESASASAALEEQASLLTQSVAVFTLSQTANNNVRQNPASSARLSLLTPNLSGSKAGKGGAQGTDLTENWETF
>CP032296.1|AYA06654.1|1915178_1916849_+|HAMP-domain-containing-protein
MFKHIRVSTCMFLLLIVFFVMQLVNSGLSLQAAHTDKLNFEQISNTAEQRDALSQSWSYLLQTRNTLNRAATRLALKQPQENITQLMSDAKASLKKAEDAYAAFLALPRRTEKAVALTAVNKASYQQLHDMLQTLTELLSSGDMNGFLNGPAQKYQDQFQKDVGDYTAYVSARFGEAQTQTAASYQNVMINTSVALVLLIALTVLALVVTRRILFTPLTIMRGHFDRIGAGDISSPIVAEGRYEIKVMLESLQQMQTSLADTVRTVRHGADTMFSGLKGIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNAENARQATQLARDASNTAEKGGELTGNVVKTMNDIAGSSKKISAITSVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVRTLAQRSAQAAKEIKALIDESVSRVSQGSQLVESAGNTMDDIVRAVTRVTDIMGEIASASDEQSRGIEQVSIAVTQMDQVTQQNATLVQQAASSTHALEIESENLTRAVSVFRLATTDGAGQRRSGLAQGLTSRQVAQPALLPATAGAVSADNWETF
>CP032296.1|AYA06655.1|1916911_1917784_+|protein-glutamate-O-methyltransferase-CheR
MNQPGSPMSADNTSIMAQMIQRLPLSDTHFRRISELIYQRAGIVLADHKREMVYNRLVRRLRILGLNDFTSYMALLENDSNSPEWQAFINALTTNLTAFFREAHHFPILAEHARSRPNSYSVWSTAASTGEEPYSIAMTLSEALGPRMASCRIQASDIDTQVLEKATAGVYRLEELRTLSPQQLQKFFLKGTGPHSGLVRVRPELAQMVAFQQLNLLANQWQLNGPFDAIFCRNVMIYFDKETQEKILRRFVPLLKPGGLMFAGHSENFSQISREFYLRGQTVYGLAKER
>CP032296.1|AYA06656.1|1917783_1918833_+|chemotaxis-response-regulator-protein-glutamate-methylesterase
MNKIRVLSVDDSALMRQLMTEIINSHPDMEMVATAPDPLVARDLIKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEITLRALELGAIDFVTKPQLGIREGMLAYSEMIADKIRTASKARLNKSVNIDQPRILSHTPLLSSEKLIAIGSSTGGTEAIRHVLQPLPPTCPALMITQHMPPGFTRSFADRLNKLCQITVKEAEDGERVLPGHAYIAPGDRHMELTRSGANYQVKLNDGPAVNRHRPSVDVLFRSVAQFAGRNAVGVILTGMGNDGAAGMLEMHRAGAYTLAQNEASCVVFGMPREAIATGGVNEVVDLSQISQRMLAQISAGQALRI
>CP032296.1|AYA06657.1|1918879_1919269_+|chemotaxis-protein-CheY
MADPNLRFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALNKLRAGGFDFVVSDWNMPNMDGLDLLKTIRADAALGKLPVLMVTAEAKKENIVAAAQAGASGYVVKPFTAATLEEKLGKIFEKLGM
>CP032296.1|AYA06658.1|1919279_1919924_+|protein-phosphatase-CheZ
MAEIPMPANDAATAGEIIARIGQLTRMLRDSMRELGLDQAIAQAAEAIPDARDRLDYVVTMTAQAAERALNCVEAAQPRQNKLESAAKKLDKRWDEWFENPIELSDARELVTDTRSYLKEIPEHTSFTNAQLMEIMMAQDFQDLTGQVIKRMMTVVQEIENQLLMVLMENIPEQQAKAKRENDSLLNGPQLDSNGVGVVASQEQVDDLLDSLGF

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Crispr_ID: CP032296_2

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

CP032296_2

2943080-2943165

Orphan

Consensus_repeat	Method
CCCTTCGCAGGGGGAGGAGCTAAAA	CRISPRCasFinder

1 spacers

The CRISPR arrays of CP032296_2

>merge|CP032296|2|2943080-2943165|CRISPRCasFinder
CCCTTCGCAGGGGGAGGAGCTAAAACCAAACTCTGGTCAGATTTAACTGGCGTGCTTCCTTCCCTTCGCAGGGGGAGGAGCTAAAA

>CP032296|2|2|2943080-2943165|CRISPRCasFinder
CCCTTCGCAGGGGGAGGAGCTAAAA	CCAAACTCTGGTCAGATTTAACTGGCGTGCTTCCTT
CCCTTCGCAGGGGGAGGAGCTAAAA

Protein	Signature genes	Signature genes Name	Protein_function
CP032296.1\|AYA07519.1\|2936568_2937681_-\|glycosyltransferase	unknown	unknown	gnl\|CDD\|182148
CP032296.1\|AYA07516.1\|2933960_2934794_+\|2-pyrone-4,6-dicarboxylate-hydrolase	unknown	unknown	gnl\|CDD\|238636
CP032296.1\|AYA07521.1\|2939423_2940170_+\|4-carboxy-2-hydroxymuconate-hydratase	unknown	unknown	gnl\|CDD\|225031
CP032296.1\|AYA07523.1\|2940901_2942026_+\|4-oxalomesaconate-tautomerase	unknown	unknown	gnl\|CDD\|380230
CP032296.1\|AYA09385.1\|2954325_2955435_-\|sn-glycerol-3-phosphate-ABC-transporter-ATP-binding-protein-UgpC	unknown	unknown	gnl\|CDD\|236947
CP032296.1\|AYA07520.1\|2938042_2939272_-\|LysR-family-transcriptional-regulator	unknown	unknown	gnl\|CDD\|176126
CP032296.1\|AYA07527.1\|2945152_2946064_-\|D,D-dipeptide-ABC-transporter-permease	unknown	unknown	gnl\|CDD\|182131
CP032296.1\|AYA07518.1\|2935322_2936576_-\|O-antigen-ligase-family-protein	unknown	unknown	gnl\|CDD\|225844
CP032296.1\|AYA07528.1\|2946068_2947085_-\|ABC-transporter-permease	unknown	unknown	gnl\|CDD\|223674
CP032296.1\|AYA07526.1\|2944122_2945151_-\|ABC-transporter-ATP-binding-protein	unknown	unknown	gnl\|CDD\|223521
CP032296.1\|AYA07515.1\|2932701_2933964_+\|gallate-dioxygenase	unknown	unknown	gnl\|CDD\|184006
CP032296.1\|AYA07531.1\|2949384_2950257_-\|MurR/RpiR-family-transcriptional-regulator	unknown	unknown	gnl\|CDD\|224651
CP032296.1\|AYA07529.1\|2947146_2948727_-\|ABC-transporter-substrate-binding-protein	unknown	unknown	gnl\|CDD\|173877
CP032296.1\|AYA07532.1\|2950989_2952648_-\|alpha-glucosidase	unknown	unknown	gnl\|CDD\|200472
CP032296.1\|AYA07517.1\|2934810_2935251_+\|type-II-3-dehydroquinate-dehydratase	unknown	unknown	gnl\|CDD\|235443
CP032296.1\|AYA07525.1\|2943166_2944123_-\|ABC-transporter-ATP-binding-protein	unknown	unknown	gnl\|CDD\|226967
CP032296.1\|AYA07533.1\|2952647_2954303_-\|alpha-glucosidase	unknown	unknown	gnl\|CDD\|200472
CP032296.1\|AYA07522.1\|2940166_2940892_+\|4-carboxy-4-hydroxy-2-oxoadipate-aldolase/oxaloacetate-decarboxylase	unknown	unknown	gnl\|CDD\|181735
CP032296.1\|AYA07524.1\|2942018_2942903_+\|NAD(P)-dependent-oxidoreductase	unknown	unknown	gnl\|CDD\|224995
CP032296.1\|AYA07530.1\|2948774_2949356_-\|D-alanyl-D-alanine-dipeptidase	unknown	unknown	gnl\|CDD\|236662

Protein	Function_ID	Function_description	E-value
CP032296.1\|AYA07519.1\|2936568_2937681_-\|glycosyltransferase	gnl\|CDD\|182148	PRK09922, PRK09922, lipopolysaccharide 1,6-galactosyltransferase.	1.23389e-101
CP032296.1\|AYA07516.1\|2933960_2934794_+\|2-pyrone-4,6-dicarboxylate-hydrolase	gnl\|CDD\|238636	cd01311, PDC_hydrolase, 2-pyrone-4,6-dicarboxylic acid (PDC) hydrolase hydrolyzes PDC to yield 4-oxalomesaconic acid (OMA) or its tautomer, 4-carboxy-2-hydroxymuconic acid (CHM). This reaction is part of the protocatechuate (PCA) 4,5-cleavage pathway. PCA is one of the most important intermediate metabolites in the bacterial pathways for various phenolic compounds, including lignin, which is the most abundant aromatic material in nature.	2.4736e-50
CP032296.1\|AYA07521.1\|2939423_2940170_+\|4-carboxy-2-hydroxymuconate-hydratase	gnl\|CDD\|225031	COG2120, COG2120, Uncharacterized proteins, LmbE homologs [Function unknown].	1.99766e-54
CP032296.1\|AYA07523.1\|2940901_2942026_+\|4-oxalomesaconate-tautomerase	gnl\|CDD\|380230	NF033377, OMA_tautomer, 4-oxalomesaconate tautomerase.	0
CP032296.1\|AYA09385.1\|2954325_2955435_-\|sn-glycerol-3-phosphate-ABC-transporter-ATP-binding-protein-UgpC	gnl\|CDD\|236947	PRK11650, ugpC, sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC.	0
CP032296.1\|AYA07520.1\|2938042_2939272_-\|LysR-family-transcriptional-regulator	gnl\|CDD\|176126	cd08435, PBP2_GbpR, The C-terminal substrate binding domain of galactose-binding protein regulator contains the type 2 periplasmic binding fold. Galactose-binding protein regulator (GbpR), a member of the LysR family of bacterial transcriptional regulators, regulates the expression of chromosomal virulence gene chvE. The chvE gene is involved in the uptake of specific sugars, in chemotaxis to these sugars, and in the VirA-VirG two-component signal transduction system. In the presence of an inducing sugar such as L-arabinose, D-fucose, or D-galactose, GbpR activates chvE expression, while in the absence of an inducing sugar, GbpR represses expression. The topology of this substrate-binding domain is most similar to that of the type 2 periplasmic binding proteins (PBP2), which are responsible for the uptake of a variety of substrates such as phosphate, sulfate, polysaccharides, lysine/arginine/ornithine, and histidine. The PBP2 bind their ligand in the cleft between these domains in a manner resembling a Venus flytrap. After binding their specific ligand with high affinity, they can interact with a cognate membrane transport complex comprised of two integral membrane domains and two cytoplasmically located ATPase domains. This interaction triggers the ligand translocation across the cytoplasmic membrane energized by ATP hydrolysis.	7.12023e-51
CP032296.1\|AYA07527.1\|2945152_2946064_-\|D,D-dipeptide-ABC-transporter-permease	gnl\|CDD\|182131	PRK09881, PRK09881, D,D-dipeptide ABC transporter permease.	8.49957e-178
CP032296.1\|AYA07518.1\|2935322_2936576_-\|O-antigen-ligase-family-protein	gnl\|CDD\|225844	COG3307, RfaL, Lipid A core - O-antigen ligase and related enzymes [Cell envelope biogenesis, outer membrane].	2.01129e-07
CP032296.1\|AYA07528.1\|2946068_2947085_-\|ABC-transporter-permease	gnl\|CDD\|223674	COG0601, DppB, ABC-type dipeptide/oligopeptide/nickel transport systems, permease components [Amino acid transport and metabolism / Inorganic ion transport and metabolism].	7.03515e-131
CP032296.1\|AYA07526.1\|2944122_2945151_-\|ABC-transporter-ATP-binding-protein	gnl\|CDD\|223521	COG0444, DppD, ABC-type dipeptide/oligopeptide/nickel transport system, ATPase component [Amino acid transport and metabolism / Inorganic ion transport and metabolism].	2.46631e-155
CP032296.1\|AYA07515.1\|2932701_2933964_+\|gallate-dioxygenase	gnl\|CDD\|184006	PRK13367, PRK13367, gallate dioxygenase.	0
CP032296.1\|AYA07531.1\|2949384_2950257_-\|MurR/RpiR-family-transcriptional-regulator	gnl\|CDD\|224651	COG1737, RpiR, Transcriptional regulators [Transcription].	1.7105e-18
CP032296.1\|AYA07529.1\|2947146_2948727_-\|ABC-transporter-substrate-binding-protein	gnl\|CDD\|173877	cd08512, PBP2_NikA_DppA_OppA_like_7, The substrate-binding component of an uncharacterized ABC-type nickel/dipeptide/oligopeptide-like import system contains the type 2 periplasmic binding fold. This CD represents the substrate-binding domain of an uncharacterized ATP-binding cassette (ABC) type nickel/dipeptide/oligopeptide-like transporter. The oligopeptide-binding protein OppA and the dipeptide-binding protein DppA show significant sequence similarity to NikA, the initial nickel receptor. The DppA binds dipeptides and some tripeptides and is involved in chemotaxis toward dipeptides, whereas the OppA binds peptides of a wide range of lengths (2-35 amino acid residues) and plays a role in recycling of cell wall peptides, which precludes any involvement in chemotaxis. Most of other periplasmic binding proteins are comprised of only two globular subdomains corresponding to domains I and III of the dipeptide/oligopeptide binding proteins. The structural topology of these domains is most similar to that of the type 2 periplasmic binding proteins (PBP2), which are responsible for the uptake of a variety of substrates such as phosphate, sulfate, polysaccharides, lysine/arginine/ornithine, and histidine. The PBP2 bind their ligand in the cleft between these domains in a manner resembling a Venus flytrap. After binding their specific ligand with high affinity, they can interact with a cognate membrane transport complex comprised of two integral membrane domains and two cytoplasmically located ATPase domains. This interaction triggers the ligand translocation across the cytoplasmic membrane energized by ATP hydrolysis. Besides transport proteins, the PBP2 superfamily includes the ligand-binding domains from ionotropic glutamate receptors, LysR-type transcriptional regulators, and unorthodox sensor proteins involved in signal transduction.	0
CP032296.1\|AYA07532.1\|2950989_2952648_-\|alpha-glucosidase	gnl\|CDD\|200472	cd11333, AmyAc_SI_OligoGlu_DGase, Alpha amylase catalytic domain found in Sucrose isomerases, oligo-1,6-glucosidase (also called isomaltase; sucrase-isomaltase; alpha-limit dextrinase), dextran glucosidase (also called glucan 1,6-alpha-glucosidase), and related proteins. The sucrose isomerases (SIs) Isomaltulose synthase (EC 5.4.99.11) and Trehalose synthase (EC 5.4.99.16) catalyze the isomerization of sucrose and maltose to produce isomaltulose and trehalulose, respectively. Oligo-1,6-glucosidase (EC 3.2.1.10) hydrolyzes the alpha-1,6-glucosidic linkage of isomaltooligosaccharides, pannose, and dextran. Unlike alpha-1,4-glucosidases (EC 3.2.1.20), it fails to hydrolyze the alpha-1,4-glucosidic bonds of maltosaccharides. Dextran glucosidase (DGase, EC 3.2.1.70) hydrolyzes alpha-1,6-glucosidic linkages at the non-reducing end of panose, isomaltooligosaccharides and dextran to produce alpha-glucose.The common reaction chemistry of the alpha-amylase family enzymes is based on a two-step acid catalytic mechanism that requires two critical carboxylates: one acting as a general acid/base (Glu) and the other as a nucleophile (Asp). Both hydrolysis and transglycosylation proceed via the nucleophilic substitution reaction between the anomeric carbon, C1 and a nucleophile. Both enzymes contain the three catalytic residues (Asp, Glu and Asp) common to the alpha-amylase family as well as two histidine residues which are predicted to be critical to binding the glucose residue adjacent to the scissile bond in the substrates. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase.	0
CP032296.1\|AYA07517.1\|2934810_2935251_+\|type-II-3-dehydroquinate-dehydratase	gnl\|CDD\|235443	PRK05395, PRK05395, type II 3-dehydroquinate dehydratase.	2.14168e-86
CP032296.1\|AYA07525.1\|2943166_2944123_-\|ABC-transporter-ATP-binding-protein	gnl\|CDD\|226967	COG4608, AppF, ABC-type oligopeptide transport system, ATPase component [Amino acid transport and metabolism].	3.75248e-114
CP032296.1\|AYA07533.1\|2952647_2954303_-\|alpha-glucosidase	gnl\|CDD\|200472	cd11333, AmyAc_SI_OligoGlu_DGase, Alpha amylase catalytic domain found in Sucrose isomerases, oligo-1,6-glucosidase (also called isomaltase; sucrase-isomaltase; alpha-limit dextrinase), dextran glucosidase (also called glucan 1,6-alpha-glucosidase), and related proteins. The sucrose isomerases (SIs) Isomaltulose synthase (EC 5.4.99.11) and Trehalose synthase (EC 5.4.99.16) catalyze the isomerization of sucrose and maltose to produce isomaltulose and trehalulose, respectively. Oligo-1,6-glucosidase (EC 3.2.1.10) hydrolyzes the alpha-1,6-glucosidic linkage of isomaltooligosaccharides, pannose, and dextran. Unlike alpha-1,4-glucosidases (EC 3.2.1.20), it fails to hydrolyze the alpha-1,4-glucosidic bonds of maltosaccharides. Dextran glucosidase (DGase, EC 3.2.1.70) hydrolyzes alpha-1,6-glucosidic linkages at the non-reducing end of panose, isomaltooligosaccharides and dextran to produce alpha-glucose.The common reaction chemistry of the alpha-amylase family enzymes is based on a two-step acid catalytic mechanism that requires two critical carboxylates: one acting as a general acid/base (Glu) and the other as a nucleophile (Asp). Both hydrolysis and transglycosylation proceed via the nucleophilic substitution reaction between the anomeric carbon, C1 and a nucleophile. Both enzymes contain the three catalytic residues (Asp, Glu and Asp) common to the alpha-amylase family as well as two histidine residues which are predicted to be critical to binding the glucose residue adjacent to the scissile bond in the substrates. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase.	0
CP032296.1\|AYA07522.1\|2940166_2940892_+\|4-carboxy-4-hydroxy-2-oxoadipate-aldolase/oxaloacetate-decarboxylase	gnl\|CDD\|181735	PRK09262, PRK09262, hypothetical protein; Provisional.	2.85054e-149
CP032296.1\|AYA07524.1\|2942018_2942903_+\|NAD(P)-dependent-oxidoreductase	gnl\|CDD\|224995	COG2084, MmsB, 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases [Lipid metabolism].	2.55617e-26
CP032296.1\|AYA07530.1\|2948774_2949356_-\|D-alanyl-D-alanine-dipeptidase	gnl\|CDD\|236662	PRK10178, PRK10178, D-alanyl-D-alanine dipeptidase; Provisional.	2.37942e-103

>CP032296.1|AYA07524.1|2942018_2942903_+|NAD(P)-dependent-oxidoreductase
MNNVTRITLIGLGEVGTILATDLKKQNPAPDIVAFDKLTDSAVIRQRARDAGVHFAPSLEDAVKGSQIIFSAVTAAEALNVAGAVARHLAPGQFFLDLNSVSPHTKREAMALIEQAGGNYIDVAVMAPYPPARLKTPLLLGGTHVYAINEWLNETGFNSKVHSNTVGEASAIKMCRSVMIKGLEALTAECLSAARQYGVEKEVLASLHASFPSLGWDGPFPHYLISRIAEHGKRRAEEMREVVKTLEDVGVAPNLSRGTVLAQQGLVDALAAKGIRYSDLEPFDWGRTVDLLRK
>CP032296.1|AYA07523.1|2940901_2942026_+|4-oxalomesaconate-tautomerase
MPQTRIPCLLMRGGTSKGAFFLADDLPHEQALRDNVLLAAMGSPDPRQIDGLGGADPLTSKVAIIRPSARPDADVDYLFAQVNVDKAVVDYGQNCGNILAAVGPFAIERQLLSAVEGITPVRIFMENTGQIATAHVPVKEGQVCYQGELRIDGVPGTAAKISVEFADIAGSSCGALLPTGNARDTFDGIDVTCIDNGMPVILLRAADFGRSGYESREQLDADTELKKRLESIRLQAGPKMKLGDVTQRTVPKMTLIAEPRNGGAISSRTFIPHRCHASIGVLGAVSVASACLIPGSITEGLAHTPSGNTPLISVEHPAGEFSVELQLDPTKTGAERLAGCALLRTARLIFEGRVAIPASIWDGHQDDQQGSHHE
>CP032296.1|AYA07522.1|2940166_2940892_+|4-carboxy-4-hydroxy-2-oxoadipate-aldolase/oxaloacetate-decarboxylase
MSLVGKKGVVVRHIPRANKADVSALAAFGVATVHEAQGRKGLLDAGIRPIQQDCAISGSAVTVLTAPGDNWMFHVAVEQCQPGDILVVAPTSPCSDGFFGDLLATSLKARGVVGLIADLGVRDTKTLRDMGFRVWSRAVHAQGTIKETLGSVNVPVVCAGQLVNPGDAIVADDDGVVVIPRQEVAQVAQASREREALEESKRARMADGELGLDIYNMRPRLAEKGLKYFDSLEELENAARR
>CP032296.1|AYA07521.1|2939423_2940170_+|4-carboxy-2-hydroxymuconate-hydratase
MTQPKKENTKSALVVSAHSADFVWRAGGAIARHTQAGYAVHIVCLSFGERGESAKLWRKGNMTEEVVKASRREEAMAAAEILGASVEFFDMGDYPLRADKESLFRLADVYRRVQPHFVLTHSLKDPYNYDHPMATHLAQEARIIAQAEGYKPGETIVGAPPVYCFEPHQPEQCDWKPDVLLDITDVWDKKYQAIQCMAGQEHLWEYYTRVAQQRGVQAKRNVGITSTKNIVYGEGYQSLFPRVTEDLA
>CP032296.1|AYA07520.1|2938042_2939272_-|LysR-family-transcriptional-regulator
MAESEMLSNLMQIRAFCQVVDQGSVSRAADELYRTQSAVTRAIRDLEQSLDVPLFERHANGMLLTDFGKCVLPRARRAIAELRQIPSQLAKLQGKSGQRRGDTEPLYLFNVRRLQIFISLCKTRHMQTVATLLGLSQPAVSAALKVLENGAGVALLERTPHGMMPSLAGQDIVPNLRRALNELRHIPADIAARRGVLEGTVQVGALPLGRTRLLPQAIIGLTQRYSGVKVVTNESAFSALASELRSGEVDFIFGALRSRDYAADMATETLFTESMVILARKGHPLLAENVTQETLREARWVLPRAETPARHLLDNSFAAMGIPPPDPVVESGDLGIVRGLLLGSDMLAAVSARQLEHELESGELVKLPLDLADTHRAIGLTFRAGSLLSPAAEALVMEIRKVCGNPIFI
>CP032296.1|AYA07519.1|2936568_2937681_-|glycosyltransferase
MITKEKEVINKKKIVLVTDLLIGRGGMENVTSQVISALKKSEEFEAGFFVINSGEKTYSKSWLDNAVLFESVCTLRNKKVKNFIHTLRLLFFIRKYKPEHVITLNTIPSLMARRAITFSAHKTVLSTWMHLPPRERYRPHYLLSAEHHFAISDEIKNQLTELGVDKNHVDVVFNPVKKTDTVILRPESLRILYIGRIHFERQKQLKDLFDSLARVNVRWSLDIVGDGEDLAICQSYTQQSGISDQITWHGWQSDPWEYVENNIKEVTCLVMTSNFEGFPLVLLEAISRGIYCVSSDCISGPSEIIENNINGQLYPQNDIIRLSQIINKIGSDRVLPEQAVIKESIGKFYEDAYMNNFKNILNKIAEGKDE
>CP032296.1|AYA07518.1|2935322_2936576_-|O-antigen-ligase-family-protein
MNSKSATAPLKIIVNASVIALCISLSLSMFITGWPQKIFYVVSYVSFFTTLYAIYKKDISFKNEHFYLVMFFSLFFLAAVRFVWAMMLKHNGIDPSPTSAGIINNYLVGSKRILLGAFVILCLSVYGSLTTQKTIFLSKVIIVLGLIVTLGFGLHEHFYTVNDRIKLTTDAASMSSYMIIFIYAAYLGLSRLENHRYSKCLDLGAFILTFILLYLCGTRITILALIFLKLTFIFLEYKIDLRKNWQMAGLVVLAIVTIIGITGHRWAQGINDIQKYDVQSSTSLGARVAIWESGIYLLHHHVGFTTPDERTTLARAFIKTHHPENTEGYTNVQYNMHNEFLEVTTLQGLLGTFSLLLPYLVLIYGWVRKVNIKGMVLPFIALFVTGLTDSVILYDQTVTLFVISLAVCCIGTRKSAE
>CP032296.1|AYA07517.1|2934810_2935251_+|type-II-3-dehydroquinate-dehydratase
MNYNIAVFNGPNLNLLGTREPETYGHDTLATIEKLCHQTGENCGAKIEFFQTNHEGDLIDRIQACRGKVDAIVLNAAAWTHTSVAIRDAVTAVSLPLYEVHITNVHKRDAFRHHSFLSDVAVGVICGFGIQGYEYAIRQAVVNLNK
>CP032296.1|AYA07516.1|2933960_2934794_+|2-pyrone-4,6-dicarboxylate-hydrolase
MTIFEQPKIDTHHHVFDPARFPYLPDTPYRPEGHEIGTTEYYRAVMQAYNIRHSLIVGPTSGYNTDSACLLDALQKGNGRFKGIAVVPFDIGIEQLANLKNAGVVGIAMNVAMLGTESFLHLDGLMGRLAELDMFAAIQVQNDQLLALLPLINRTRTRLLIDHSGRPDVTQGLQQPAFQALLSLADQQRAWVKLSGLAKFSRQPFPYQDGHAYQHALLDAFGAEHCMWGSDWPFLRAEHRMDLGTLLMLVGQLFPDEKIRQQILWQTPKDLFGFTDY
>CP032296.1|AYA07515.1|2932701_2933964_+|gallate-dioxygenase
MAKIIGGLAVSHTPTIGFAVDHNKQEESAWAPIFESFAPMQKWLDEKKPDVLVYIFNDHVTSFFFDHYSAFTLGIDEQYEVADEGGGPRDLPAVKGHAALSQHIGASLMADEFDMSFFQDKPLDHGLFSPLSALLPHEGGWPTQIVPLQIGVLQFPIPTARRCYKLGQALRRAIESFPEDLNVAIVATGGVSHQVHGERCGFNNPDWDEQFVDMIVNDPERLTEMTLAEFATLGGMEGAEVIMWLVMRGALSANVKKLHQAYYLPSMTGIATLILENQSREAPYDVQQRQRDKIAQQMAGVEKLPGTYPFTQARSLKALRINRFLHRLIQPAWRARFLTDQHALFAENKLTEEEQQLLRELDWRGMIHYGVSFFLLEKLGAVVGVSNLHIYSAMRGETLEEFQKTRNQQVLYSVAGKAKA
>CP032296.1|AYA07525.1|2943166_2944123_-|ABC-transporter-ATP-binding-protein
MMAEALVELQDVRVRFAASYNWRGKPTSYVHALNGLDLNIIRGETLGIVGESGCGKSTLAQLLMGLQKPSSGEVHRARHDNSWFGSGMQMVFQDPQSSLDPRLPVWRIITEPVFVQKHNSSRERRELAATLAEQVGIRREYLDRLPHEFSGGQRQRISIARALSSEPDIIVLDEPTSALDISVQAQILNLLVELQRQRNLTYVLISHNVSVVRHMSDRVAVMYLGQIVELGTAEKVLSEPQHPYTRLLLDSVPRAGRQLDEGIVDKKGELPSNRNLPMGCYFRERCPLAGDGCQMPQDLVHGAGGVVARCHRVMPERV
>CP032296.1|AYA07526.1|2944122_2945151_-|ABC-transporter-ATP-binding-protein
MTEQSQAPVLEIDNLQLVFPVYGGEVKALNQVSLSVNAGEIVGVVGESGSGKSVTAMMAMRLLPPDGFTVTGGALRMLGTDVIHASEEQMRTLRGARVAMIFQEPMNALNPTRKIGRQMCEVICLHQKLSKAQAWQKAIGLLQDMQIADAEQVMSRYPFELSGGMRQRVLIAMAFSCEPELIIADEPTTALDVTVQRQVLRLLQRKARASGTAVLFITHDMAVVSQLCDRVYVMYAGHVIESGNTTDVIDTPSHPYSIGLLQAAPENGEPRSLLKAIPGTVPNLAKLPQGCAFRGRCNHADDRCIATPALASLPHFPQQSVACWHPQRYAGDIHIQRIEEHS
>CP032296.1|AYA07527.1|2945152_2946064_-|D,D-dipeptide-ABC-transporter-permease
MSVIEPGVNNHRADIVAPDVKPRFANWRRTFYLLSRSPLTMLGLAIMLIVLFIMIFSPWLVPHDPNAISLTDRLQAPSALHWFGTDEVGRDLFSRVLIGSQQSVAAGLAVVIIAGTTGSLLGCFSGVVGGVVDALIMRCMDIMLSVPSLVLTMALAAALGPSLFNAMLAIAVVRIPFYVRLARGQTLMLRHQAYMQATRTFGASRWHLITWHVLRNVMPPLVVQASLDIGTSILMAATLGFIGLGAQQPTAEWGAMVSNGRNYILDQWWYSAFPGVAILITATGFNLFGDGLRDLLDPKSRGR
>CP032296.1|AYA07528.1|2946068_2947085_-|ABC-transporter-permease
MTFWTLIRQRCWGLILVMAGVCVITFIISHMIPGDPARLLAGDRASNEMVEHIREQLGLNQPLYVQFFRYVSDLLHGDLGTSIRTGRPVLEDLKAFFPATLELAVSALFLAIVLGIPLGVLSAVYRNKFPDHLVRLLSVLGISTPAFWLGLGVIILFYGHLNLLPGGGRLDDWMDPPANITGFYVLDSLLTGNRDAFWNSLQHLAMPALTLAFVHMGIVARQIRSAMLEQLSEDYIRTARANGLSRWRVILSHALPNALIPSVTVLGLAMGDLLYGAVLTETVFAWPGMGAYVVNSIQALDFPAVMGFAVVVSFAYVIVNLCVDLLYVWIDPRIGREG
>CP032296.1|AYA07529.1|2947146_2948727_-|ABC-transporter-substrate-binding-protein
MSNTAINTFRKNTKTQVAGAIFLALSGLSFATQAAVPKDMLVIGKAADPQTLDPAVTIDNNDWTVTYPAYQRLVQYKTEDGKGSTQVEGDLAASWTASPDQLIWTFKLKPGAKFDDGSDVNADAVKWSFERLMKIGQGPSEAFPKDMTVTVVDPMTVTFTLKTPFAPFLYTLANDGAGIVNPAIAKANPADEGKAWLAGHTAGSGPYKLDRWQKGQQLVLVPNPHYSGAKPAFKRVTVKIIGESATRRLQLSRGDLDIADSLPMDQLAALKKENKVAVEEFPSLRVTYLYLNNGKTPLNQVDLRRAISSAVDYQGMVKGILGGNAKQMRGPIPDGMWGFDPTAKQYTLDLTQAKADLEKVKDKPQTLDFLYSDNDPNWESIALSVQASLGQAGINVKLEKLANATMRDRIGQGDYDISIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYSNPDVDALLKKAVSVTDQKQRTDYYQQAQKIVIDEAAYVYLFQKNYQVAMNKDVKGFVFNPMLEQVFNVGQMHKQ
>CP032296.1|AYA07530.1|2948774_2949356_-|D-alanyl-D-alanine-dipeptidase
MNTKTPDVALIDLAEILPDAVIDLKYATADNLTGQPIYRESLCLLHPDAASALVRCNEVAKLAGFTLKIYDAYRPQAAQACLWAAFPNPDYVVDIRLGSHHSRGVAVDLTLLDEQGEAVDMGAGFDEMTEVSHPFYPDLPPHIQRNRLLLNAVMAAGGFKGITSEWWHFELPDAKTYPLLEERFGCYPPVNAA
>CP032296.1|AYA07531.1|2949384_2950257_-|MurR/RpiR-family-transcriptional-regulator
MQPAQDVIEQIEASFAGQTPTGKRIASYLLANLAQIPFETADSIARSAATSGISVGRYLRSLGYRNLDHFKQTLRLQSGTSYQPWVVTDRLGAYRERSKQPQQEKLQQSLTLELEAINHVYRLAQTDSFAQVSRRLADAEAVFILGIQSVRGIANNFSSYLEYLRPKVYFADGLSGTYVESLNSEFAQPYLVVTDTRAYSKMAQTYCEAACERGLNVALITDIYCPWARDYPVDLLQVKTDTGQFWDSMAPLSCLFNLLCSAVLELRGHDVEQRLANNRILQKQLGQFES
>CP032296.1|AYA07532.1|2950989_2952648_-|alpha-glucosidase
MANWWKEAVAYQIYPRSFKDSNRDGIGDINGITEKLDYLKDLGIDLIWICPIYQSPNDDNGYDISDYQQIMAEFGTMADFDHLLKQVHLRGMRLIIDLVVNHTSDEHPWFVESGSSLDNPKRDWYIWRDGKQGGEPNNWESIFNGSAWKHSAATGQYFLHLFSERQPDLNWENPGVRSAIYTMMRWWLDKGVDGFRIDAICHMKKEPAFSDMPNPQGLPYVPSFERHLNYDGLLDYVDDMCEQVLSRYDIVTVGEMNGVTAEQGEEWVGEQRGRLNMIFQFEHVKLWQDGQHTKAQSGLDLMGLKDIFTRWQTLLENKGWNALYVENHDLPRVVSGWGDAENYHRESATAIAAMYFLMKGTPFIYQGQELGMTNTHFASLDDFDDVAARKRASEMRRQGMEESDILAILSRTGRDNSRTPMQWDHSIHGGFSSVTPWFPANSNYREINVAQQREDNHSVLNFYRALIRLRRQMPALIEGNYQLLLPSHPHIYAYLRTLENQQILVIGNFSACPQEVDSQQLALEGAQLLLGNYQDEGALHILRPYETRIYQR
>CP032296.1|AYA07533.1|2952647_2954303_-|alpha-glucosidase
MSDMHSAQQWKDAVVYQVYPRSFMDSNGDGTGDLNGIISKLDYLQQLGINMLWLSPVYRSPMDDNGYDISDYENIADIFGSMAEMEHLIAEAEARDIGILMDLVVNHTSDEHPWFIDALTSKNSAYRDFYIWRKPAADGGPPNDQRSHFGGSAWTLDKASGEYYLHQFSTRQPDLNWENPDVRAAIHAMMNRWLARGIGGFRMDVIDLIGKDVDQQIMANGKNLHLYLHQMNRATFGPYGSITVGETWSATPEDALLYSAEARQELTMVFQFEHIRLFWDEHHGKWRNKPFDLLRFKAVIDKWQTALAHRGWNSLFWSNHDLPRAVSKFGDEGQYRVESAKMLATALHCLKGTPYIYQGEEIGMTNVRFPRIDDYRDIESLNLYQERVAEGVSHEEMMRGIHANGRDNARTPMQWDSSQHAGFTTSQPWIDVNPNYKQINVAAALDDPNSVFYHYQKLVALRKQQPLLVHGDFRQIFNSHPQVFAWLRTSGDQTLVVINNFTREPLTLDVPDNLQSLQGQCLISNYAPREQLGARLQLQPYESFALLIGSL
>CP032296.1|AYA09385.1|2954325_2955435_-|sn-glycerol-3-phosphate-ABC-transporter-ATP-binding-protein-UgpC
MAQLTLDKIQKRYGAKTEAIKSLDLHIKSGEFVVIVGPSGCGKSTLLRMIAGLEEISGGGMYIDGNYVNDDTPAERGIGMVFQSYALYPHMSVYQNMAFGLELARHPASEIDQRVRACARILQLEPLLARRPKDLSGGQRQRVAIGRAIVREPRLFLFDEPLSNLDASLRVQMRMEVSALHKRLGVTIIYVTHDQVEAMTLADRIVVLNQGNIEQVGTPLELYDQPANEFVAQFIGSPKMNLLPATLRQAGEQQSVVELDNGKALVLPVATPAAAQGRSVNLGIRPEHIRSGNREECEYQGEVMFVEQMGNETLLYLDNGNASEPWVVRHAERSAIQVGEIVGVRLPVERCYLFDSHGRAFHRHLTMNH

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Click the colored protein region to show detailed information

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity
CP032296_1	1.4\|1909459\|32\|CP032296\|PILER-CR,CRISPRCasFinder,CRT	1909459-1909490	32	HQ634191	Cyanophage Syn10 genomic sequence	5793-5824	6	0.812
CP032296_1	1.4\|1909459\|32\|CP032296\|PILER-CR,CRISPRCasFinder,CRT	1909459-1909490	32	DQ149023	Synechococcus cyanophage syn9, complete genome	144228-144259	6	0.812
CP032296_1	1.4\|1909459\|32\|CP032296\|PILER-CR,CRISPRCasFinder,CRT	1909459-1909490	32	NC_008296	Synechococcus phage syn9, complete genome	144228-144259	6	0.812
CP032296_1	1.4\|1909459\|32\|CP032296\|PILER-CR,CRISPRCasFinder,CRT	1909459-1909490	32	KY653124	Staphylococcus phage IME1367_01, complete genome	38501-38532	7	0.781
CP032296_1	1.4\|1909459\|32\|CP032296\|PILER-CR,CRISPRCasFinder,CRT	1909459-1909490	32	AB182649	Bacteriophage phi WMY holWMY, lysWMY gene for holin, lysin, complete cds	38-69	7	0.781
CP032296_1	1.2\|1909339\|32\|CP032296\|PILER-CR,CRISPRCasFinder,CRT	1909339-1909370	32	NC_010553	Burkholderia ambifaria MC40-6 plasmid pBMC401, complete sequence	300310-300341	8	0.75

1. spacer 1.4|1909459|32|CP032296|PILER-CR,CRISPRCasFinder,CRT matches to HQ634191 (Cyanophage Syn10 genomic sequence) position: , mismatch: 6, identity: 0.812

gttactgatgtcgtaatcaataatcgactgaa---	CRISPR spacer
gtcactgatgttgtaatcaataa---actgaggac	Protospacer
**.********.***********   *****.

2. spacer 1.4|1909459|32|CP032296|PILER-CR,CRISPRCasFinder,CRT matches to DQ149023 (Synechococcus cyanophage syn9, complete genome) position: , mismatch: 6, identity: 0.812

gttactgatgtcgtaatcaataatcgactgaa---	CRISPR spacer
gtcactgatgttgtaatcaataa---actgaggac	Protospacer
**.********.***********   *****.

3. spacer 1.4|1909459|32|CP032296|PILER-CR,CRISPRCasFinder,CRT matches to NC_008296 (Synechococcus phage syn9, complete genome) position: , mismatch: 6, identity: 0.812

gttactgatgtcgtaatcaataatcgactgaa---	CRISPR spacer
gtcactgatgttgtaatcaataa---actgaggac	Protospacer
**.********.***********   *****.

4. spacer 1.4|1909459|32|CP032296|PILER-CR,CRISPRCasFinder,CRT matches to KY653124 (Staphylococcus phage IME1367_01, complete genome) position: , mismatch: 7, identity: 0.781

gttactgatgtcgtaatcaataatcgactgaa--	CRISPR spacer
gttactgatgtgttaatcaataat--acaggttc	Protospacer
***********  ***********  ** *.

5. spacer 1.4|1909459|32|CP032296|PILER-CR,CRISPRCasFinder,CRT matches to AB182649 (Bacteriophage phi WMY holWMY, lysWMY gene for holin, lysin, complete cds) position: , mismatch: 7, identity: 0.781

gttactgatgtcgtaatcaataatcgactgaa--	CRISPR spacer
gttactgatgtgttaatcaataat--acaggttc	Protospacer
***********  ***********  ** *.

6. spacer 1.2|1909339|32|CP032296|PILER-CR,CRISPRCasFinder,CRT matches to NC_010553 (Burkholderia ambifaria MC40-6 plasmid pBMC401, complete sequence) position: , mismatch: 8, identity: 0.75

gctgagtgcacggtgttgatcgcattctcaat---	CRISPR spacer
gctgcgttcacggtgttgatcgcg---ccgatccc	Protospacer
**** ** ***************.   .*.**

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

589630 : 663230

Cronobacter_phage(61.11%)

terminase,portal,tRNA,capsid,integrase,tail,holin,head

attL 622827:622874|attR 655021:655068

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYA05528.1\|589630_590947_+\|integrase	site-specific integrase	A0A248SL35	Klebsiella_phage	6.0e-35	30.0
AYA09296.1\|591011_591566_+	cell filamentation protein Fic	NA	NA	NA	NA
AYA05529.1\|591562_591721_-	hypothetical protein	NA	NA	NA	NA
AYA05530.1\|591939_593562_+	hypothetical protein	NA	NA	NA	NA
AYA05531.1\|593857_594646_+	hypothetical protein	NA	NA	NA	NA
AYA05532.1\|594859_595096_+	AlpA family phage regulatory protein	NA	NA	NA	NA
AYA05533.1\|595227_596640_+	DUF3987 domain-containing protein	NA	NA	NA	NA
AYA05534.1\|596861_597485_+	inovirus Gp2 family protein	NA	NA	NA	NA
AYA09297.1\|597574_599113_-	hypothetical protein	NA	NA	NA	NA
AYA05535.1\|599167_599356_-	hypothetical protein	NA	NA	NA	NA
AYA05536.1\|599370_601284_-	DUF262 domain-containing protein	NA	NA	NA	NA
AYA05537.1\|601270_602611_-	DUF262 domain-containing protein	NA	NA	NA	NA
AYA05538.1\|602908_604774_-	hypothetical protein	NA	NA	NA	NA
AYA05539.1\|604958_608078_-	type I restriction endonuclease subunit R	NA	NA	NA	NA
AYA05540.1\|608070_609267_-	anticodon nuclease	NA	NA	NA	NA
AYA05541.1\|609268_610510_-	restriction endonuclease subunit S	NA	NA	NA	NA
AYA05542.1\|610506_612066_-	type I restriction-modification system subunit M	A0A2H4PQP4	Staphylococcus_phage	2.5e-104	42.5
AYA05543.1\|612320_613184_+	GTPase family protein	NA	NA	NA	NA
AYA05544.1\|613271_614090_+	DUF945 domain-containing protein	A0A2C9CX26	Yersinia_phage	2.0e-44	37.0
AYA05545.1\|614333_615044_+	WYL domain-containing protein	NA	NA	NA	NA
AYA05546.1\|615087_615624_+	DUF4339 domain-containing protein	NA	NA	NA	NA
AYA05547.1\|615674_616112_+	hypothetical protein	NA	NA	NA	NA
AYA05548.1\|616179_616578_+	hypothetical protein	A0A1B2IBY1	Erwinia_phage	1.2e-26	45.0
AYA09298.1\|616962_618021_-	HNH endonuclease	A0A1S6KZY3	Salmonella_phage	2.2e-11	34.6
AYA05549.1\|618198_620193_-	nuclease	A0A2H4J1E0	uncultured_Caudovirales_phage	3.1e-27	32.9
AYA05550.1\|620564_621044_+	DNA repair protein RadC	NA	NA	NA	NA
AYA05551.1\|621057_621393_+	type IV toxin-antitoxin system YeeU family antitoxin	NA	NA	NA	NA
AYA05552.1\|621459_621780_+	toxin	NA	NA	NA	NA
AYA05553.1\|621893_622727_+	DUF4942 domain-containing protein	NA	NA	NA	NA
622827:622874	attL	NA	NA	NA	NA
AYA05554.1\|622990_624760_-	hypothetical protein	NA	NA	NA	NA
AYA05555.1\|624761_625790_-\|integrase	site-specific integrase	A0A218M4I3	Erwinia_phage	1.8e-119	64.4
AYA05556.1\|625792_626374_-	phage repressor protein	Q94N02	Haemophilus_virus	1.0e-23	35.2
AYA05557.1\|626514_626736_+	regulator	NA	NA	NA	NA
AYA05558.1\|626765_627275_+	hypothetical protein	F1BUN6	Cronobacter_phage	1.9e-42	52.8
AYA05559.1\|627284_627488_+	DUF2724 domain-containing protein	NA	NA	NA	NA
AYA05560.1\|627490_627817_+	hypothetical protein	NA	NA	NA	NA
AYA05561.1\|627824_628256_+	hypothetical protein	NA	NA	NA	NA
AYA05562.1\|628329_628590_+	DUF2732 family protein	NA	NA	NA	NA
AYA05563.1\|628590_630747_+	replication endonuclease	F1BUM9	Cronobacter_phage	1.2e-223	60.5
AYA05564.1\|630848_631058_+	hypothetical protein	NA	NA	NA	NA
AYA05565.1\|631037_631298_-	transcriptional regulator	F1BUM8	Cronobacter_phage	9.9e-27	62.8
AYA05566.1\|631348_632380_-\|portal	phage portal protein	F1BUM7	Cronobacter_phage	7.2e-137	66.5
AYA05567.1\|632376_634164_-\|terminase	terminase	F1BUM5	Cronobacter_phage	1.4e-252	71.7
AYA05568.1\|634314_635154_+\|capsid	phage capsid protein	F1BUM4	Cronobacter_phage	2.1e-49	52.2
AYA05569.1\|635219_636254_+\|capsid	phage major capsid protein, P2 family	F1BUM2	Cronobacter_phage	1.1e-137	75.4
AYA05570.1\|636256_636970_+\|terminase	terminase	F1BUM0	Cronobacter_phage	6.7e-73	60.3
AYA05571.1\|636966_637158_+	hypothetical protein	NA	NA	NA	NA
AYA05572.1\|637251_637704_+\|head	head completion/stabilization protein	F1BUL8	Cronobacter_phage	3.4e-46	61.3
AYA05573.1\|637700_638207_+\|tail	phage tail protein	F1BUL7	Cronobacter_phage	9.0e-40	48.8
AYA05574.1\|638203_638893_+	hypothetical protein	F1BUL6	Cronobacter_phage	6.6e-78	65.9
AYA05575.1\|638902_640030_+	DUF2586 domain-containing protein	F1BUL5	Cronobacter_phage	8.7e-144	69.8
AYA05576.1\|640032_640485_+	DUF2597 family protein	F1BUL4	Cronobacter_phage	1.6e-51	65.1
AYA05577.1\|640498_640795_+\|holin	holin	C7BGD7	Burkholderia_phage	1.3e-19	54.3
AYA05578.1\|640791_641133_+	M15 family peptidase	F1BUL3	Cronobacter_phage	9.9e-43	78.2
AYA05579.1\|641129_641504_+	hypothetical protein	F1BUL2	Cronobacter_phage	1.8e-21	54.4
AYA05580.1\|641448_641628_+	hypothetical protein	F1BUL1	Cronobacter_phage	9.6e-13	61.8
AYA05581.1\|641624_641882_+	hypothetical protein	A5X9I7	Aeromonas_virus	2.2e-18	55.8
AYA05582.1\|642069_644379_+\|tail	phage tail tape measure protein	F1BUK9	Cronobacter_phage	1.8e-164	45.0
AYA05583.1\|644378_644711_+	DUF2590 family protein	F1BUK8	Cronobacter_phage	9.1e-33	71.7
AYA05584.1\|644703_645888_+	hypothetical protein	F1BUK6	Cronobacter_phage	1.5e-162	73.4
AYA05585.1\|645880_646468_+	hypothetical protein	F1BUK5	Cronobacter_phage	6.0e-80	72.8
AYA05586.1\|649177_649618_+\|tail	tail fiber assembly protein	B6SCW7	Bacteriophage	6.2e-21	44.1
AYA05587.1\|649607_650330_+	hypothetical protein	NA	NA	NA	NA
AYA05588.1\|650304_650844_+	hypothetical protein	F1BUJ9	Cronobacter_phage	1.6e-47	59.7
AYA05589.1\|650847_652503_+	hypothetical protein	F1BUJ7	Cronobacter_phage	2.6e-181	57.6
AYA05590.1\|652565_652760_-	hypothetical protein	NA	NA	NA	NA
AYA05591.1\|653069_653342_+	hypothetical protein	NA	NA	NA	NA
AYA05592.1\|653439_654270_-	hypothetical protein	NA	NA	NA	NA
AYA05593.1\|654524_654893_-	hypothetical protein	NA	NA	NA	NA
AYA05594.1\|655242_655746_+	G/U mismatch-specific DNA glycosylase	NA	NA	NA	NA
655021:655068	attR	NA	NA	NA	NA
AYA05595.1\|656024_656516_+	hypothetical protein	NA	NA	NA	NA
AYA05596.1\|656596_657199_+	protein rhiA	NA	NA	NA	NA
AYA05597.1\|657232_657700_+	hypothetical protein	NA	NA	NA	NA
AYA05598.1\|657760_659599_-	RNA polymerase sigma factor RpoD	A0A2I7SAT0	Vibrio_phage	1.9e-34	33.3
AYA05599.1\|659801_661550_-	DNA primase	A0A0K1LMQ9	Caulobacter_phage	3.5e-75	39.5
AYA05600.1\|661685_661901_-	30S ribosomal protein S21	NA	NA	NA	NA
AYA05601.1\|662216_663230_+\|tRNA	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD	A0A0R6PI74	Moraxella_phage	3.3e-110	60.1

DBSCAN-SWA_2

739167 : 807275

Erwinia_phage(26.47%)

tRNA,tail,plate

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYA09303.1\|739167_740217_+\|tRNA	tRNA pseudouridine(13) synthase TruD	NA	NA	NA	NA
AYA09302.1\|740194_740962_+	5'/3'-nucleotidase SurE	A0A1B1ITZ2	uncultured_Mediterranean_phage	6.3e-69	53.7
AYA05665.1\|740951_741578_+	protein-L-isoaspartate(D-aspartate) O-methyltransferase	A0A1B1IU40	uncultured_Mediterranean_phage	8.8e-37	51.2
AYA09304.1\|741838_742843_+	murein hydrolase activator NlpD	D7RWE0	Brochothrix_phage	2.2e-05	35.2
AYA05666.1\|742894_743881_+	RNA polymerase sigma factor RpoS	F4YCU2	Synechococcus_phage	7.1e-33	33.1
AYA05667.1\|743979_746535_-	DNA mismatch repair protein MutS	A0A1V0SJ67	Klosneuvirus	6.4e-25	24.7
AYA05668.1\|746957_749618_+	hypothetical protein	NA	NA	NA	NA
AYA05669.1\|749772_750876_+	murein transglycosylase B	NA	NA	NA	NA
AYA05670.1\|751037_751532_+	nicotinamide-nucleotide amidase	B5TK85	Pseudomonas_phage	1.7e-27	48.7
AYA05671.1\|751639_752704_+	DNA recombination/repair protein RecA	A0A2D1GPX2	Mycobacterium_phage	3.2e-111	61.6
AYA05672.1\|752824_753514_+	recombination regulator RecX	NA	NA	NA	NA
AYA05673.1\|753433_753715_-	hypothetical protein	NA	NA	NA	NA
AYA05674.1\|753652_756280_+\|tRNA	alanine--tRNA ligase	A0A1V0SK38	Klosneuvirus	5.5e-80	38.8
AYA05675.1\|756536_756722_+	carbon storage regulator	A0A2I7RT00	Vibrio_phage	4.9e-12	66.7
AYA05676.1\|758020_758587_+	fructose-1-phosphate/6-phosphogluconate phosphatase	NA	NA	NA	NA
AYA05677.1\|758583_759012_+	DedA family protein	NA	NA	NA	NA
AYA09305.1\|759097_760669_+	glutamate--cysteine ligase	NA	NA	NA	NA
AYA05678.1\|760826_761342_+	S-ribosylhomocysteine lyase	NA	NA	NA	NA
AYA05679.1\|761411_762701_-	DUF21 domain-containing protein	NA	NA	NA	NA
AYA05680.1\|762717_763509_-	inner membrane protein YpjD	NA	NA	NA	NA
AYA05681.1\|763673_765035_+	signal recognition particle protein	NA	NA	NA	NA
AYA05682.1\|765213_765462_+	30S ribosomal protein S16	NA	NA	NA	NA
AYA05683.1\|765480_766029_+	ribosome maturation factor RimM	NA	NA	NA	NA
AYA05684.1\|766096_766870_+\|tRNA	tRNA (guanosine(37)-N1)-methyltransferase TrmD	NA	NA	NA	NA
AYA05685.1\|766918_767266_+	50S ribosomal protein L19	NA	NA	NA	NA
AYA05686.1\|767357_767537_-	late control protein B	F1BUT0	Erwinia_phage	6.4e-17	75.5
AYA05687.1\|767601_768702_-\|tail	phage tail protein	Q6K1G4	Salmonella_virus	4.4e-84	43.9
AYA05688.1\|768704_769175_-\|tail	phage tail protein	U5N3F6	Enterobacteria_phage	1.7e-37	50.0
AYA05689.1\|769171_770803_-\|tail	phage tail tape measure protein	F1BUT7	Erwinia_phage	1.2e-16	28.6
AYA05690.1\|770795_770930_-\|tail	GpE family phage tail protein	O80316	Escherichia_phage	6.7e-11	78.9
AYA05691.1\|770950_771238_-\|tail	phage tail assembly protein	A0A0F7LDQ8	Escherichia_phage	4.3e-23	60.9
AYA05692.1\|771297_771807_-\|tail	phage major tail tube protein	A0A218M4J0	Erwinia_phage	3.1e-64	68.4
AYA05693.1\|771821_772991_-\|tail	phage tail sheath protein	F1BUU3	Erwinia_phage	8.4e-182	80.5
AYA05694.1\|773132_773681_-\|tail	tail fiber assembly protein	F1BUK2	Cronobacter_phage	2.7e-34	42.1
AYA05695.1\|773677_775054_-	hypothetical protein	A0A0F7LDR4	Escherichia_phage	5.4e-63	60.9
AYA05696.1\|775065_775593_-\|tail	phage tail protein I	U5N0U8	Enterobacteria_phage	5.6e-69	69.2
AYA05697.1\|775585_776494_-\|plate	baseplate assembly protein	S4TNY7	Salmonella_phage	8.4e-121	75.8
AYA05698.1\|776499_776850_-\|plate	baseplate assembly protein	F1BUP4	Erwinia_phage	9.9e-38	63.8
AYA05699.1\|776846_777488_-\|plate	phage baseplate assembly protein V	A0A0F7LBP2	Escherichia_phage	6.4e-67	60.4
AYA05700.1\|777569_778031_-\|tail	phage tail protein	F1BUP9	Erwinia_phage	1.1e-23	43.3
AYA05701.1\|778123_778552_-	transcriptional regulator	F1BUQ1	Erwinia_phage	1.4e-06	48.3
AYA05702.1\|778552_779062_-	lysozyme	A0A218M4K3	Erwinia_phage	1.5e-55	61.1
AYA05703.1\|779042_779264_-	hypothetical protein	NA	NA	NA	NA
AYA05704.1\|779254_779458_-\|tail	phage tail protein	F1BUQ5	Erwinia_phage	8.6e-18	56.7
AYA05705.1\|779669_779864_-	hypothetical protein	NA	NA	NA	NA
AYA05706.1\|780012_781905_-	replication endonuclease	Q7Y4B8	Escherichia_virus	5.3e-178	50.3
AYA05707.1\|781998_782220_-	conjugal transfer protein TraR	A0A1S6L007	Salmonella_phage	4.6e-09	44.3
AYA05708.1\|782219_782495_-	hypothetical protein	NA	NA	NA	NA
AYA05709.1\|782627_783218_+	CI repressor	Q6K1G0	Salmonella_virus	1.8e-52	51.3
AYA05710.1\|783506_784583_+	3-deoxy-7-phosphoheptulonate synthase	A0A0B6VT43	Edwardsiella_phage	3.9e-85	48.3
AYA05711.1\|784589_785711_+	bifunctional chorismate mutase/prephenate dehydrogenase	NA	NA	NA	NA
AYA05712.1\|785788_786943_-	bifunctional chorismate mutase/prephenate dehydratase	NA	NA	NA	NA
AYA05713.1\|787254_787599_-	ribosome-associated translation inhibitor RaiA	NA	NA	NA	NA
AYA05714.1\|787880_788615_-	outer membrane protein assembly factor BamD	NA	NA	NA	NA
AYA05715.1\|788745_789723_+	23S rRNA pseudouridine(1911/1915/1917) synthase RluD	NA	NA	NA	NA
AYA05716.1\|789722_790460_+	peptidoglycan editing factor PgeF	NA	NA	NA	NA
AYA05717.1\|790590_793164_+	ATP-dependent chaperone ClpB	H6X3M6	Enterobacteria_phage	2.2e-126	35.1
AYA05718.1\|798993_800292_+	MFS transporter	Q6JIH2	Burkholderia_virus	2.9e-42	30.3
AYA05719.1\|800481_801840_-	CDP-diacylglycerol--serine O-phosphatidyltransferase	NA	NA	NA	NA
AYA05720.1\|801921_804642_-	bifunctional acyl-CoA synthetase/GNAT family N-acetyltransferase	NA	NA	NA	NA
AYA05721.1\|804673_805378_-	DTW domain-containing protein	NA	NA	NA	NA
AYA05722.1\|805507_805927_-	thioredoxin TrxC	A0A191VYI2	Roseobacter_phage	4.5e-13	34.4
AYA05723.1\|806171_807275_+\|tRNA	tRNA/rRNA methyltransferase	NA	NA	NA	NA

DBSCAN-SWA_3

1873122 : 1883384

Enterobacteria_phage(37.5%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYA06620.1\|1873122_1873764_+	uridine kinase	A0A1V0SAA3	Catovirus	3.2e-34	37.9
AYA06621.1\|1873801_1874383_+	dCTP deaminase	I4AZP2	Saccharomonospora_phage	1.5e-30	41.6
AYA06622.1\|1874426_1876277_+	outer membrane assembly protein AsmA	NA	NA	NA	NA
AYA06623.1\|1876355_1877945_-	TerC family protein	A0A0R6PEZ3	Moraxella_phage	8.2e-39	43.6
AYA06624.1\|1878407_1879295_+	UTP--glucose-1-phosphate uridylyltransferase	A0A127AW70	Bacillus_phage	1.2e-60	46.9
AYA06625.1\|1879410_1880283_-	glucose-1-phosphate thymidylyltransferase	I7I009	Enterobacteria_phage	1.0e-107	65.6
AYA06626.1\|1880302_1881367_-	dTDP-glucose 4,6-dehydratase	I7HTA3	Enterobacteria_phage	1.7e-101	53.7
AYA06627.1\|1881978_1882515_+	dTDP-4-dehydrorhamnose 3,5-epimerase	I7HJC4	Enterobacteria_phage	5.5e-56	57.0
AYA06628.1\|1882511_1883384_+	dTDP-4-dehydrorhamnose reductase	K7QJU0	Escherichia_phage	6.3e-41	37.5

DBSCAN-SWA_4

2814826 : 2822962

Cronobacter_phage(57.14%)

tail,transposase

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYA07410.1\|2814826_2815414_-	hypothetical protein	F1BUK5	Cronobacter_phage	5.5e-81	73.3
AYA07411.1\|2815406_2816591_-	hypothetical protein	F1BUK6	Cronobacter_phage	9.7e-162	73.4
AYA07412.1\|2816583_2816916_-	DUF2590 family protein	F1BUK8	Cronobacter_phage	9.1e-33	71.7
AYA07413.1\|2816915_2819186_-\|tail	phage tail tape measure protein	F1BUK9	Cronobacter_phage	6.7e-159	44.4
AYA07414.1\|2819259_2820379_+\|transposase	IS3-like element ISRaq1 family transposase	S5WIU1	Leptospira_phage	5.1e-51	42.4
AYA07415.1\|2820389_2820929_-	FRG domain-containing protein	Q9AZ51	Lactococcus_phage	1.0e-04	34.2
AYA07416.1\|2820919_2821240_-	hypothetical protein	NA	NA	NA	NA
AYA07417.1\|2821325_2821691_-	bleomycin resistance protein	NA	NA	NA	NA
AYA07418.1\|2821852_2822962_-	porin	Q1MVN1	Enterobacteria_phage	9.6e-111	57.4

DBSCAN-SWA_5

3053343 : 3061826

Tupanvirus(33.33%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYA07618.1\|3053343_3055326_-	bifunctional UDP-4-amino-4-deoxy-L-arabinose formyltransferase/UDP-glucuronic acid oxidase ArnA	A0A2K9KZK0	Tupanvirus	2.8e-20	24.6
AYA07619.1\|3055325_3056306_-	undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferase	A8CG95	Salmonella_phage	5.4e-33	32.4
AYA07620.1\|3056305_3057448_-	UDP-4-amino-4-deoxy-L-arabinose aminotransferase	A0A2K9L0G1	Tupanvirus	5.0e-30	28.9
AYA07621.1\|3057796_3058546_-	vitamin B12 ABC transporter ATP-binding protein BtuD	A0A2R8FG22	Brazilian_cedratvirus	7.1e-09	29.8
AYA07622.1\|3058565_3059117_-	glutathione peroxidase	A0A1S7DMQ0	Molluscum_contagiosum_virus	9.2e-14	41.6
AYA07623.1\|3059189_3060197_-	vitamin B12 ABC transporter permease BtuC	NA	NA	NA	NA
AYA07624.1\|3060350_3060749_-	DUF2502 domain-containing protein	NA	NA	NA	NA
AYA07625.1\|3061529_3061826_-	integration host factor subunit alpha	A0A0H3UZA0	Geobacillus_virus	3.2e-13	40.0

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

Overview of predicted results

Overview of the results

Cas Category Instructions

Results visualization

1. CP032297

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection

2. CP032296

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Click the colored protein region to show detailed information

Click the colored protein region to show detailed information

Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection