CRISPRimmunity

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Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Self targeting spacer number	Target MGE spacer number	Prophage number	Anti-CRISPR protein number
AP018559	Hydrogenophilus thermoluteolus TH-1 plasmid pTH1 DNA, complete genome	0 crisprs	c2c9_V-U4,cas8e,cas1	0	0	1	0
AP018558	Hydrogenophilus thermoluteolus TH-1 DNA, complete genome	1 crisprs	DinG,cas2,cas1,cas6,csx1,cmr6gr7,cmr5gr11,cmr4gr7,cmr3gr5,cas10,cmr1gr7,RT,cas3,DEDDh,csa3	0	0	6	0

Results visualization

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CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

1784 : 34335

Burkholderia_phage(25.0%)

integrase,transposase

attL 3502:3516|attR 23567:23581

The bacterium proteins that are colored denote the protein is present at specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin' and 'tRNA')

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
BBD78350.1\|1784_2951_+\|transposase	transposase	A0A0K2CZ57	Paenibacillus_phage	1.8e-67	40.0
3502:3516	attL	NA	NA	NA	NA
BBD78351.1\|3610_3850_+	hypothetical protein	NA	NA	NA	NA
BBD78352.1\|3979_4306_-	growth inhibitor PemK	A0A2K9V2U7	Faecalibacterium_phage	7.6e-08	35.5
BBD78353.1\|4292_4523_-	addiction module antitoxin	NA	NA	NA	NA
BBD78354.1\|4596_6261_-	mobilization protein A	M1Q738	Cellulophaga_phage	4.7e-37	36.0
BBD78355.1\|6250_6634_-	plasmid mobilization relaxosome protein MobC	NA	NA	NA	NA
BBD78356.1\|7518_7662_+\|transposase	transposase	U5N3V8	Enterobacteria_phage	2.7e-10	88.9
BBD78357.1\|7669_7921_-\|transposase	transposase	NA	NA	NA	NA
BBD78358.1\|8027_8354_-	MazF/PemK family toxin	NA	NA	NA	NA
BBD78359.1\|8350_8572_-	hypothetical protein	NA	NA	NA	NA
BBD78360.1\|8728_10003_-\|transposase	transposase	A0A7P9	Microcystis_virus	8.3e-66	40.0
BBD78361.1\|9996_10626_-	resolvase	A0A1S5RFV4	Helicobacter_phage	1.4e-63	62.8
BBD78362.1\|10828_11242_+	hypothetical protein	NA	NA	NA	NA
BBD78363.1\|11195_11516_+	transcriptional regulator	NA	NA	NA	NA
BBD78364.1\|11628_12159_-	type I-E CRISPR-associated protein Cse1/CasA	NA	NA	NA	NA
BBD78365.1\|12811_13048_+	CopG family transcriptional regulator	NA	NA	NA	NA
BBD78366.1\|13044_13476_+	PIN domain-containing protein	NA	NA	NA	NA
BBD78367.1\|13685_14111_+	toxin-antitoxin system toxin component PIN family	NA	NA	NA	NA
BBD78368.1\|14120_14372_+	hypothetical protein	NA	NA	NA	NA
BBD78369.1\|14375_14981_+\|integrase	integrase	A0A2L1IV36	Escherichia_phage	6.3e-32	42.6
BBD78370.1\|15027_15720_-	hypothetical protein	NA	NA	NA	NA
BBD78371.1\|15878_16328_-	hypothetical protein	NA	NA	NA	NA
BBD78372.1\|16482_16689_+	hypothetical protein	NA	NA	NA	NA
BBD78373.1\|16694_17822_-\|transposase	transposase	NA	NA	NA	NA
BBD78374.1\|18186_20478_-	hypothetical protein	NA	NA	NA	NA
BBD78375.1\|20725_20962_+	prevent-host-death family protein	NA	NA	NA	NA
BBD78376.1\|20965_21418_+	PIN domain nuclease	NA	NA	NA	NA
BBD78377.1\|21526_21787_-	CopG family transcriptional regulator	NA	NA	NA	NA
BBD78378.1\|21804_22461_-	chromosome partitioning protein ParA	E5FFJ3	Burkholderia_phage	2.8e-57	56.3
BBD78379.1\|22526_23021_-	RES domain-containing protein	NA	NA	NA	NA
BBD78380.1\|23020_23473_-	antitoxin	NA	NA	NA	NA
BBD78381.1\|23616_23883_-	cryptic plasmid protein A	NA	NA	NA	NA
23567:23581	attR	NA	NA	NA	NA
BBD78382.1\|24261_25635_-\|transposase	transposase	NA	NA	NA	NA
BBD78383.1\|25792_26776_+\|transposase	transposase	E5E3P6	Burkholderia_phage	1.3e-111	61.0
BBD78384.1\|27027_27576_-\|transposase	transposase	NA	NA	NA	NA
BBD78385.1\|27640_29530_+	site-specific DNA-methyltransferase	A0A2K5B2C1	Erysipelothrix_phage	1.2e-116	38.3
BBD78386.1\|29532_32208_+	DEAD/DEAH box helicase	A0A2K5B256	Erysipelothrix_phage	1.4e-155	37.1
BBD78387.1\|32595_33387_-\|transposase	transposase	NA	NA	NA	NA
BBD78388.1\|33876_34335_+\|transposase	transposase	E5E3P6	Burkholderia_phage	7.1e-36	58.4

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

2. AP018558

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CRISPR-Cas detection and classification

Crispr_ID: AP018558_1

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

AP018558_1

755081-755611

TypeIII

Consensus_repeat	Method
GTTGGAATCATGCCCTGATTGAGAAGGGATTAAGAC	PILER-CR
GTTGGAATCATGCCCTGATTGAGAAGGGATTAAGAC	CRISPRCasFinder
GTTGGAATCATGCCCTGATTGAGAAGGGATTAAGAC	CRT

7 spacers

cas2,cas1,cas6,csx1,cmr6gr7,cmr5gr11,cmr4gr7

The CRISPR arrays of AP018558_1

>merge|AP018558|1|755081-755611|PILER-CR,CRISPRCasFinder,CRT
GTTGGAATCATGCCCTGATTGAGAAGGGATTAAGACCCGCTCTCCAGGGCGAAGGTAATACTGCGTCTCTGGTTGGAATCATGCCCTGATTGAGAAGGGATTAAGACACAAGGACGGGAAGAAACTGGTGCGCTTGTTCTGCGTTGGAATCATGCCCTGATTGAGAAGGGATTAAGACGGTTCCATCCTCACTCAAACCACCGATACGCGCAGTGTTGGAATCATGCCCTGATTGAGAAGGGATTAAGACGTCAATCCCTTTCCATTCCTCATGTTTTTCCATGTTGGAATCATGCCCTGATTGAGAAGGGATTAAGACGGCCGTTGCCTTGCGGATGACGTCCACAGATGCAGGGTTGGAATCATGCCCTGATTGAGAAGGGATTAAGACAGCGCGCTAACGGGAATTATCACGCAAACCTCGTGGTTGGAATCATGCCCTGATTGAGAAGGGATTAAGACGCGATTCGACAAAGCATGTGCGTGCGTTTTCAAGTTGGAATCATGCCCTAATTGAGAAGGGATTAATTT

>AP018558|1|1|755081-755542|PILER-CR
GTTGGAATCATGCCCTGATTGAGAAGGGATTAAGAC	CCGCTCTCCAGGGCGAAGGTAATACTGCGTCTCTG
GTTGGAATCATGCCCTGATTGAGAAGGGATTAAGAC	ACAAGGACGGGAAGAAACTGGTGCGCTTGTTCTGC
GTTGGAATCATGCCCTGATTGAGAAGGGATTAAGAC	GGTTCCATCCTCACTCAAACCACCGATACGCGCAGT
GTTGGAATCATGCCCTGATTGAGAAGGGATTAAGAC	GTCAATCCCTTTCCATTCCTCATGTTTTTCCAT
GTTGGAATCATGCCCTGATTGAGAAGGGATTAAGAC	GGCCGTTGCCTTGCGGATGACGTCCACAGATGCAGG
GTTGGAATCATGCCCTGATTGAGAAGGGATTAAGAC	AGCGCGCTAACGGGAATTATCACGCAAACCTCGTG
GTTGGAATCATGCCCTGATTGAGAAGGGATTAAGAC

>AP018558|1|1|755081-755611|CRISPRCasFinder
GTTGGAATCATGCCCTGATTGAGAAGGGATTAAGAC	CCGCTCTCCAGGGCGAAGGTAATACTGCGTCTCTG
GTTGGAATCATGCCCTGATTGAGAAGGGATTAAGAC	ACAAGGACGGGAAGAAACTGGTGCGCTTGTTCTGC
GTTGGAATCATGCCCTGATTGAGAAGGGATTAAGAC	GGTTCCATCCTCACTCAAACCACCGATACGCGCAGT
GTTGGAATCATGCCCTGATTGAGAAGGGATTAAGAC	GTCAATCCCTTTCCATTCCTCATGTTTTTCCAT
GTTGGAATCATGCCCTGATTGAGAAGGGATTAAGAC	GGCCGTTGCCTTGCGGATGACGTCCACAGATGCAGG
GTTGGAATCATGCCCTGATTGAGAAGGGATTAAGAC	AGCGCGCTAACGGGAATTATCACGCAAACCTCGTG
GTTGGAATCATGCCCTGATTGAGAAGGGATTAAGAC	GCGATTCGACAAAGCATGTGCGTGCGTTTTCAA
GTTGGAATCATGCCCTAATTGAGAAGGGATTAATTT

>AP018558|1|1|755081-755611|CRT
GTTGGAATCATGCCCTGATTGAGAAGGGATTAAGAC	CCGCTCTCCAGGGCGAAGGTAATACTGCGTCTCTG
GTTGGAATCATGCCCTGATTGAGAAGGGATTAAGAC	ACAAGGACGGGAAGAAACTGGTGCGCTTGTTCTGC
GTTGGAATCATGCCCTGATTGAGAAGGGATTAAGAC	GGTTCCATCCTCACTCAAACCACCGATACGCGCAGT
GTTGGAATCATGCCCTGATTGAGAAGGGATTAAGAC	GTCAATCCCTTTCCATTCCTCATGTTTTTCCAT
GTTGGAATCATGCCCTGATTGAGAAGGGATTAAGAC	GGCCGTTGCCTTGCGGATGACGTCCACAGATGCAGG
GTTGGAATCATGCCCTGATTGAGAAGGGATTAAGAC	AGCGCGCTAACGGGAATTATCACGCAAACCTCGTG
GTTGGAATCATGCCCTGATTGAGAAGGGATTAAGAC	GCGATTCGACAAAGCATGTGCGTGCGTTTTCAA
GTTGGAATCATGCCCTAATTGAGAAGGGATTAATTT

Protein	Signature genes	Signature genes Name	Protein_function
AP018558.1\|BBD76989.1\|763753_764689_-\|type-III-B-CRISPR-module-RAMP-protein-Cmr4	cmr4gr7	cd09682_cmr4gr7_CAS-III-B	gnl\|CDD\|274210
AP018558.1\|BBD76979.1\|754646_754931_+\|CRISPR-associated-protein-Cas2	cas2	pfam09827_cas2_CAS-I:CAS-II:CAS-III	gnl\|CDD\|187856
AP018558.1\|BBD76984.1\|759799_761278_-\|CRISPR-associated-protein-Csx16	csx1	cd09732_csx1_CAS-III	gnl\|CDD\|187863
AP018558.1\|BBD76978.1\|753619_754636_+\|CRISPR-associated-endonuclease-Cas1	cas1	cd09634_cas1_CAS-I:CAS-II:CAS-III:CAS-V	gnl\|CDD\|376633
AP018558.1\|BBD76983.1\|758582_759791_-\|CRISPR-associated-protein	csx1	cd09668_csx1_CAS-III	gnl\|CDD\|274040
AP018558.1\|BBD76980.1\|755880_757044_+\|transposase	unknown	unknown	gnl\|CDD\|227946
AP018558.1\|BBD76987.1\|762903_763326_+\|PIN-domain-containing-protein	unknown	unknown	gnl\|CDD\|224485
AP018558.1\|BBD76982.1\|757638_758586_-\|CRISPR-associated-protein-Cas6	cas6	mkCas0066_cas6_CAS-I:CAS-III	gnl\|CDD\|187890
AP018558.1\|BBD76977.1\|753323_753608_+\|CRISPR-associated-protein-Cas2	cas2	pfam09827_cas2_CAS-I:CAS-II:CAS-III	gnl\|CDD\|187856
AP018558.1\|BBD76975.1\|751343_752441_+\|amino-acid-ABC-transporter-permease	unknown	unknown	gnl\|CDD\|223836
AP018558.1\|BBD76972.1\|747192_748884_+\|long-chain-fatty-acid--CoA-ligase	unknown	unknown	gnl\|CDD\|235923
AP018558.1\|BBD76974.1\|750152_751328_+\|amino-acid-ABC-transporter-permease	unknown	unknown	gnl\|CDD\|226960
AP018558.1\|BBD76985.1\|761274_762636_-\|type-III-B-CRISPR-module-RAMP-protein-Cmr6	cmr6gr7	cd09661_cmr6gr7_CAS-III-B	gnl\|CDD\|187792
AP018558.1\|BBD76973.1\|749040_750066_+\|amino-acid-ABC-transporter-substrate-binding-protein	unknown	unknown	gnl\|CDD\|270410
AP018558.1\|BBD76976.1\|752440_753259_+\|amino-acid-ABC-transporter-ATP-binding-protein	unknown	unknown	gnl\|CDD\|224051
AP018558.1\|BBD76988.1\|763349_763751_-\|type-III-B-CRISPR-module-associated-protein-Cmr5	cmr5gr11	pfam09701_cmr5gr11_CAS-III-B	gnl\|CDD\|378242
AP018558.1\|BBD76971.1\|746661_747111_+\|CBS-domain-containing-protein	unknown	unknown	gnl\|CDD\|341391
AP018558.1\|BBD76981.1\|757195_757516_-\|hypothetical-protein	unknown	unknown	unknown
AP018558.1\|BBD76970.1\|746277_746601_+\|ferredoxin	unknown	unknown	gnl\|CDD\|378771
AP018558.1\|BBD76986.1\|762688_762907_+\|CopG-family-transcriptional-regulator	unknown	unknown	gnl\|CDD\|226420

Protein	Function_ID	Function_description	E-value
AP018558.1\|BBD76989.1\|763753_764689_-\|type-III-B-CRISPR-module-RAMP-protein-Cmr4	gnl\|CDD\|274210	TIGR02580, putative_CRISPR-associated_protein, CRISPR type III-B/RAMP module RAMP protein Cmr4. This model represents a CRISPR-associated protein from the family that includes TM1792 of Thermotoga maritima. This family is part of the broad RAMP superfamily (pfam03787) collection of CRISPR-associated proteins. It is the fourth of a recurring set of six proteins, four of are in the RAMP superfamily, that we designate the CRISPR RAMP module.	1.08927e-71
AP018558.1\|BBD76979.1\|754646_754931_+\|CRISPR-associated-protein-Cas2	gnl\|CDD\|187856	cd09725, Cas2_I_II_III, CRISPR/Cas system-associated protein Cas2. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and associated Cas proteins comprise a system for heritable host defense by prokaryotic cells against phage and other foreign DNA; Cas2 is present in majority of CRISPR/Cas systems along with Cas1; RNAse specific to U-rich regions; Possesses an RRM/ferredoxin fold.	2.4289e-13
AP018558.1\|BBD76984.1\|759799_761278_-\|CRISPR-associated-protein-Csx16	gnl\|CDD\|187863	cd09732, Csx1_III-U, CRISPR/Cas system-associated protein Csx1. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and associated Cas proteins comprise a system for heritable host defense by prokaryotic cells against phage and other foreign DNA; Protein of this family often fused to HTH domain; Some proteins could have an additional fusion with RecB-family nuclease domain; Core domain appears to have a Rossmann-like fold; loosely associated with CRISPR/Cas systems; also known as TM1812 family.	1.61154e-49
AP018558.1\|BBD76978.1\|753619_754636_+\|CRISPR-associated-endonuclease-Cas1	gnl\|CDD\|376633	pfam01867, Cas_Cas1, CRISPR associated protein Cas1. Clustered regularly interspaced short palindromic repeats (CRISPRs) are a family of DNA direct repeats found in many prokaryotic genomes. This family of proteins corresponds to Cas1, a CRISPR-associated protein. Cas1 may be involved in linking DNA segments to CRISPR.	3.81848e-94
AP018558.1\|BBD76983.1\|758582_759791_-\|CRISPR-associated-protein	gnl\|CDD\|274040	TIGR02221, CRISPR-associated_protein_Csx1_2, CRISPR-associated protein, TM1812 family. CRISPR is a term for Clustered Regularly Interspaced Short Palidromic Repeats. A number of protein families appear only in association with these repeats and are designated Cas (CRISPR associated) proteins. This family, represented by TM1812 of Thermotoga maritima, is found also in Vibrio vulnificus YJ016, Nitrosomonas europaea ATCC 19718, a large plasmid of Synechocystis sp. PCC 6803, and Fibrobacter succinogenes S85.	9.55772e-54
AP018558.1\|BBD76980.1\|755880_757044_+\|transposase	gnl\|CDD\|227946	COG5659, COG5659, FOG: Transposase [DNA replication, recombination, and repair].	9.74593e-79
AP018558.1\|BBD76987.1\|762903_763326_+\|PIN-domain-containing-protein	gnl\|CDD\|224485	COG1569, COG1569, Predicted nucleic acid-binding protein, contains PIN domain [General function prediction only].	6.20953e-10
AP018558.1\|BBD76982.1\|757638_758586_-\|CRISPR-associated-protein-Cas6	gnl\|CDD\|187890	cd09760, Cas6_III, CRISPR/Cas system-associated RAMP superfamily protein Cas6. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and associated Cas proteins comprise a system for heritable host defense by prokaryotic cells against phage and other foreign DNA; Cas6 is an endoribonuclease that generates crRNAs, predicted subunit of Cascade complex.	3.38488e-08
AP018558.1\|BBD76977.1\|753323_753608_+\|CRISPR-associated-protein-Cas2	gnl\|CDD\|187856	cd09725, Cas2_I_II_III, CRISPR/Cas system-associated protein Cas2. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and associated Cas proteins comprise a system for heritable host defense by prokaryotic cells against phage and other foreign DNA; Cas2 is present in majority of CRISPR/Cas systems along with Cas1; RNAse specific to U-rich regions; Possesses an RRM/ferredoxin fold.	6.67311e-15
AP018558.1\|BBD76975.1\|751343_752441_+\|amino-acid-ABC-transporter-permease	gnl\|CDD\|223836	COG0765, HisM, ABC-type amino acid transport system, permease component [Amino acid transport and metabolism].	2.86896e-68
AP018558.1\|BBD76972.1\|747192_748884_+\|long-chain-fatty-acid--CoA-ligase	gnl\|CDD\|235923	PRK07059, PRK07059, Long-chain-fatty-acid--CoA ligase; Validated.	0
AP018558.1\|BBD76974.1\|750152_751328_+\|amino-acid-ABC-transporter-permease	gnl\|CDD\|226960	COG4597, BatB, ABC-type amino acid transport system, permease component [Amino acid transport and metabolism].	0
AP018558.1\|BBD76985.1\|761274_762636_-\|type-III-B-CRISPR-module-RAMP-protein-Cmr6	gnl\|CDD\|187792	cd09661, Cmr6_III-B, CRISPR/Cas system-associated RAMP superfamily protein Cmr6. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and associated Cas proteins comprise a system for heritable host defense by prokaryotic cells against phage and other foreign DNA; RAMP superfamily protein; This protein is a subunit of Cmr complex.	1.39452e-35
AP018558.1\|BBD76973.1\|749040_750066_+\|amino-acid-ABC-transporter-substrate-binding-protein	gnl\|CDD\|270410	cd13692, PBP2_BztA, Substrate bindng domain of ABC glutamate/glutamine/aspartate/asparagine transporter; the type 2 periplasmic binding protein fold. BztA is the periplamic-binding protein component of ABC transporter specific for carboxylic amino acids, glutamine and asparagine. The BZtA domain belongs to the type 2 periplasmic binding protein fold superfamily (PBP2), whose many members are involved in chemotaxis and uptake of nutrients and other small molecules from the extracellular space as a primary receptor. The PBP2 proteins are typically comprised of two globular subdomains connected by a flexible hinge and bind their ligand in the cleft between these domains in a manner resembling a Venus flytrap. After binding their specific ligand with high affinity, they can interact with a cognate membrane transport complex comprised of two integral membrane domains and two receptor cytoplasmically-located ATPase domains. This interaction triggers the ligand translocation across the cytoplasmic membrane energized by ATP hydrolysis.	4.02144e-130
AP018558.1\|BBD76976.1\|752440_753259_+\|amino-acid-ABC-transporter-ATP-binding-protein	gnl\|CDD\|224051	COG1126, GlnQ, ABC-type polar amino acid transport system, ATPase component [Amino acid transport and metabolism].	7.82906e-170
AP018558.1\|BBD76988.1\|763349_763751_-\|type-III-B-CRISPR-module-associated-protein-Cmr5	gnl\|CDD\|378242	pfam09701, Cas_Cmr5, CRISPR-associated protein (Cas_Cmr5). CRISPR is a term for Clustered, Regularly Interspaced Short Palindromic Repeats. A number of protein families appear only in association with these repeats and are designated Cas (CRISPR-Associated) proteins. This family, represented by TM1791.1 of Thermotoga maritima, is found in both archaeal and bacterial species.	7.52615e-22
AP018558.1\|BBD76971.1\|746661_747111_+\|CBS-domain-containing-protein	gnl\|CDD\|341391	cd04623, CBS_pair_bac_euk, Two tandem repeats of the cystathionine beta-synthase (CBS pair) domains present in bacteria and eukaryotes. The CBS domain, named after human CBS, is a small domain originally identified in cystathionine beta-synthase and is subsequently found in a wide range of different proteins. CBS domains usually occur in tandem repeats. They associate to form a so-called Bateman domain or a CBS pair based on crystallographic studies in bacteria. The CBS pair was used as a basis for this cd hierarchy since the human CBS proteins can adopt the typical core structure and form an intramolecular CBS pair. The interface between the two CBS domains forms a cleft that is a potential ligand binding site. The CBS pair coexists with a variety of other functional domains and this has been used to help in its classification here. It has been proposed that the CBS domain may play a regulatory role, although its exact function is unknown. Mutations of conserved residues within this domain are associated with a variety of human hereditary diseases, including congenital myotonia, idiopathic generalized epilepsy, hypercalciuric nephrolithiasis, and classic Bartter syndrome (CLC chloride channel family members), Wolff-Parkinson-White syndrome (gamma 2 subunit of AMP-activated protein kinase), retinitis pigmentosa (IMP dehydrogenase-1), and homocystinuria (cystathionine beta-synthase).	1.53862e-31
AP018558.1\|BBD76970.1\|746277_746601_+\|ferredoxin	gnl\|CDD\|378771	pfam11953, DUF3470, Domain of unknown function (DUF3470). This presumed domain is functionally uncharacterized. This domain is found in bacteria. This domain is about 50 amino acids in length. This domain is found associated with pfam00037. This domain has a single completely conserved residue N that may be functionally important.	1.60607e-18
AP018558.1\|BBD76986.1\|762688_762907_+\|CopG-family-transcriptional-regulator	gnl\|CDD\|226420	COG3905, COG3905, Predicted transcriptional regulator [Transcription].	5.21962e-05

>AP018558.1|BBD76979.1|754646_754931_+|CRISPR-associated-protein-Cas2
MATFLICYDITDPKRLARTHRYFTRVATPIQYSVFIYQGDERSLRRIMERAVCLINPKKDDLRCYPLPAGGWRKRIGRAVLPEGIHLSVLPPDL
>AP018558.1|BBD76978.1|753619_754636_+|CRISPR-associated-endonuclease-Cas1
MSSLIVDRRGVHLALDGNAIVIREDEERLATIPIQPLSRVFLKGDVSVSANLLGKLGEAGVGVIILAGRRGKPALMLARPHNDARRRVAQILKAFDEPFALEFSRALVHRKCEGQRYFLLELRQRYPGVRLELTHAIRQIEKAAVDAQSAQSIAALRGIEGHAARYYFSGLKAVVPDSWGFHGRNRRPPRDPFNALLSLTYTLMHAEIALSLFGAGFDPFVGFYHALSFGRESLASDLLEPLRVVCDRFCLELIRNETVRKEHFSQTESGCLLGKAGRQHFYGAYEHAGNDLRSAIEQEVEWLSDWLKAAEEPLAAAEEAWEAFASASDSSVASDPLE
>AP018558.1|BBD76977.1|753323_753608_+|CRISPR-associated-protein-Cas2
MDKGRDRYLVAYDICDPRRLARVHRYLNGYKVGGQKSVYEVWATPAEIATIITTLRKLIDAEVDRVHLFRLDPRMSMHAYGIPVESFPSYFVVA
>AP018558.1|BBD76976.1|752440_753259_+|amino-acid-ABC-transporter-ATP-binding-protein
MDARGEFQMNETATATTTPNRIASHKTAEAMIVMEHVNKWYGDFHVLKDINLTVARGERIVLCGPSGSGKSTTIRCINRLEAHQSGRIVVDGIELSDDVRQIEAIRKEVGMVFQHFNLFPHLTVLENCTLAPIWVKKMPKKEAEAIAMHFLERVRIPDQANKYPGQLSGGQQQRVAIARALCMKPKVMLFDEPTSALDPEMVKEVLDTMIELAEEGMTMLCVTHEMGFARTVADRVIFMDKGEIVEEAPPEVFFTNPQNERTKQFLGQILNH
>AP018558.1|BBD76975.1|751343_752441_+|amino-acid-ABC-transporter-permease
MTHETASSLQPPKVIFSPIGWMREHLFSSVWNSILTLITLYILWLLVPPVIEWAFLKASWVGTSRDDCTPGGACWVFIAQRWSQFMYGFYPEALRWRVDLAGILAVLWGGPLLFQRFPWKKLFGFSFLIGYPLIAWWLLYGGDLFGMTTVPTDQWGGLMLTLVIAAVGIAGAMPLGILFALGRRSELPVIKAVSIAFIEFWRGVPLISVLFMSSVMLPLFLPEGMNFDKLVRALIAVVLFQAAYIAEVVRGGLQAIPKGQYEAAQALGLNYWQMMGLIILPQALKLVIPGIVNTFIALFKDTSLVIIIGLSDFFMIIHRATVDPAWLGFATEGYVFAAAVYWIFCFGMSRYSIALEKRLNTDHRR
>AP018558.1|BBD76974.1|750152_751328_+|amino-acid-ABC-transporter-permease
MKPSRSAFLSWIYDPKIRGIIAQVVTLIVLGLLVGWLAHNAIVNLAARGIASGFDFLNDPAGFGILQTLIPYTESDTYARVFVIGLLNTLLVSALGIVAATILGFIMGVARLSKNALVSALATVYIETFRNIPPLLQIFFWYFAVLQAMPSPRQSLGWFDAVFLNIRGLYLPRPIPTEAVTAFVVAVVLAFVLWWVIARWAKRRQDETGQPFPVFWTGLGLVVLLPYLASLIFGSPFTWEVPRLRGFNFVGGWVMIPELVALWVALSIYTSAFIAEIVRAGILAVPHGQTEAAMALGLTSQQRLRLVILPQAMRVIIPPLTSQYLNLTKNSSLAAAIGYPDIVGLFAGTVLNQTGQAIETIAMAMAVYLTISITISLFMNWYNKRMALVER
>AP018558.1|BBD76973.1|749040_750066_+|amino-acid-ABC-transporter-substrate-binding-protein
MKQKTLLAAVGAALAVAAVAPAQAGETMDAIKARGEIRCGVSDGLPGFSYTDQKGVVRGIDADFCRALAAAVFGDANKVKFTPLTAKERFTALQSGEIDVLSRNTTWTMSRDTGMGMVFPAAVVYYDGQGFLVNKKLGVKSAKELDGATVCIQAGTTTELNLADYFRTHKMQYTPITYDKSDESAKGLEAGRCDVLTSDQSQLYAQRIKMANPDQYVVLPEVISKEPLGPVVRPGDHEWFSTVRWTHYAMLIAEELGVTSKNVDEMKKSNNPEIRRLLGVDADFGKAIGLPQDWAYQVIKQVGNYGESFERNVGKGSPLKIERGLNALWTQGGLQYAPPIR
>AP018558.1|BBD76972.1|747192_748884_+|long-chain-fatty-acid--CoA-ligase
MEKIWLKSYPEGVPAEIGPLPYSSLGDLFAQSVARYADRPAAACMGQVLTYRELDEQSRAFAAYLQQVIQLPKGARVALMMPNVLQYMVALFGVLRAGYVVVNVNPLYTPRELEHQLKDSGAEAIVILENFAHTLEAAIAHGVRVPHIIVTSLGDLFPGLKGAITNFVVRYVKKMVPAWRLPQVTRWQEALAAGAKTTLQPVAVGPEDIAFLQYTGGTTGVAKGAVLLHRNILSNVAQATAWIREAVKEGEEIVITALPLYHIFALTANCMTFTALGGLNVLIPNPRDIPGFVKELGKWRFTVMTGVNTLFNALLNHPEFAKLDFSAFKVTLGGGMAVQEAVAKRWKQVTGKPLLEAYGLTETSPAVTINPMTLQEYNHSIGLPLPSTEISIRSDAGAELPVGAEGELCVRGPQVMKEYWNRPDETAKVFTEDGFLRTGDVAKIDENGFVYLVDRKKDMILVSGFNVYPNEVEDVIALHPGVLEVAVIGVPDEKSGEVVKAFVVKKDPALTEEAIIAWCRERLTGYKVPKRVEFRDELPKTNVGKILRRALREKRAEKVAKGQ
>AP018558.1|BBD76971.1|746661_747111_+|CBS-domain-containing-protein
MKVREILAVKGNALFTVSPAATLKEAVATMVQEDIGSLVVFDGGRLVGLLTFREALKAIAQAGAAWEQRPVRETMLAAPMTVSPDTEVEPLREQMVRVHQRYFPVMDGETLLGVVSFHDVAKAVLEEQSFENRALKAFIKGIQDEEASR
>AP018558.1|BBD76970.1|746277_746601_+|ferredoxin
MTYVVTEACIKCKYTDCVDVCPVDCFREGPNFLVIDPDECIDCSLCVPECPVEAIYAEDDVPEDQREFIALNAELAKQWPPIVERHEPLPDADQWAGVKEKKHLLQR
>AP018558.1|BBD76980.1|755880_757044_+|transposase
MVGRSGIAQSARNRLSVPALDEACYWIVDETGIPKQGRHSVGVARQYCGQVGKTENCQVAVSLSVASEKGSLPIAFRLYLPESWANDPERRKKAGVPETVTFATKPEIALAQIADALSAGVTPGVVLADAAYGDSTAFRDRLTEWGLRYAVAVREATTVWPPGVEPLQPEPYTGRGRPARNLRRSPGHEPVSVKRLAESLPQSAYRTVTWREGSNGVLRSRFAAVRVRAVHRDYWRATLREPEWLLIEWPETESEPSHYFLSTLPQSYSLTQLVHTVKMRWRIERDYRELKQEVGLGHYEGRNWRGFHHHATLTIAAYGFLLLQRLKGWDKKNGLFPQAPALPHWYVPRGSPKSTTPRTRLDRNDSSSDHPSHRPRTPAMSVLWENV
>AP018558.1|BBD76981.1|757195_757516_-|hypothetical-protein
MVSTSAITYGQKVSPLQYQQFDCEQITAEMQRVQNRALQLAGRLDEAAQNDKILTGVGLILFWPALFALGGTKEQETEYARLRGEYEALQQVAIQKKCPGAISNET
>AP018558.1|BBD76982.1|757638_758586_-|CRISPR-associated-protein-Cas6
MNAPDIALPVARYRLEFEVTKDLHLPAYAGSTLRGAWGSALRAICCMTRLPVCDNCPLLDTCPYAAIFETRPHQRRASFLQNFTHVPRPYVIEPPQMGKRSYIPGERFSFHIVLVGRALEHLSLILWAYVKAFRRGVGSGDGTAELLRAIHMGETETIVLEAPGKALKPHDPAIPKTPDLRGTVTLQFHTPLRLQTNGRRATREEHTARKLLTALIRRIALLNAYHGNEPFVLDLKALANQANLITSETQLRWHDWTRYSTRQKKHMALGGVVGTWKLMGDLTPFAPFLHLGQWVHVGKEATFGLGKYHLTLAHS
>AP018558.1|BBD76983.1|758582_759791_-|CRISPR-associated-protein
MTTLISFLGKSQLDSKTGYRTARYRMPDGTIHETAYLGLALAEHLAAERVILLGTESSMWDLLVENVAGDHAADELRLELFDAVKESRVTQSLLDDVAPSMAAQLGRPVASLLIPSCATFAEQQEVLTRLADHLHQGECVVLDLTHGFRHLAMLGFAAARYLVHARSVTVHGLYYGALDMTHNGVTPVVTLDGLAHLQEWAEAFEAYEASGDFSRFAPLLVQDGFPRTAADALERAWQLLMVTNVNDAAKSLQPVLKQLGEPLSGASELFRARLLKALRWSQESTLSEKLRRLALQSLKRGDTLRASIFGLEAFLAREVETADGDPLDFAQRKAADERFQEQARDGLHPDWKREAFWLLKNVRNSCAHGTPPSHARHAQLLRNPQQLARELEATLNRLTNTP
>AP018558.1|BBD76984.1|759799_761278_-|CRISPR-associated-protein-Csx16
MKLISFLGTGKYESVCYHWEGKRYESRFVAEALARLSNVTSAVILATEGAEQTHGQALADAFANAAIDCQFKRIRNGQSPDELWDNFSILTDLLDTEEPALLDITHGFRSQPFFAGAVVAFLRTIRNKTSSVRVVYGAFEARDANNQTPVWDLTPFVELLDITQNIHIFLQSGQAENLAAHIEELGRKLACEWARTKQGPRPKLDNFAKALREFSESLTTVRVGQILLSNGRKPSKAAQLLQAIDEAKADLTQHLPPVARVLDAMRAMVEPLTPERTDLTGDEGKALMASLARLYLSLGRYAETGIVLREGWVNLYATSQATCPGQSFDKQEREKAEKRMSLAGNTERELAGLRNDIEHAGFRQQPLPPQTIREKLQRFLDTFEQAQLATKPETSEGTIWFVSRHPGAVAWAARQGLKVDRQVAHLDPDEVQAGDTVIGTLPVHLAAQVCARKARYLHLSLDLPPEARGLELSADDLERYGARLEAYHIAQE
>AP018558.1|BBD76985.1|761274_762636_-|type-III-B-CRISPR-module-RAMP-protein-Cmr6
MPIAAVPAYMGQEFKNASPGMRFGMYLAIWTDRPDQEAQVKKRAEAQSREGQELRELLRRQGMDATIADLCGQKRLPGLWEKNDHAARKAWDAIKKLSQADKALMQALAQRQETLGGKARGFSLEAKATAPFVTGLGNEHPLENGFSFLWPYGLPYLPGSGVKGVVRRAAQELAEGLWGDTRGWSLEPGYEIEVKSGKERTRIQLSVLDVLFGREPPSGDSNAVRGALSFWDVIPLIAGDSLMVEVMTPHQSHYYQQKTERKSGNSTTPHDSGQPKPIFFLTVPPGSEFKFYVVCDTAHLKRLTAHKLDGAPDLLATSDGRPLWQQLLQTAFEHAFEWLGFGAKTALGYGAMTAKPDEEATNNTQQSVFRNVAPKTNETWKQATLEYKPNTQEVMATFQNQKTAPLRQKAAQEFLDQLGEERRNRLKKKQKLANVSVIVRKQGNHFELKGIAE
>AP018558.1|BBD76986.1|762688_762907_+|CopG-family-transcriptional-regulator
MSTTLTIRLDETLDRELNRLARALHRPKSDLAREFIRRQLALAQFERARTLIEPAAEQAGFLTDEDIFREVS
>AP018558.1|BBD76987.1|762903_763326_+|PIN-domain-containing-protein
MRLFFDTNVWISAFAAKGLCRDLVRLALALHGGTRVTLVTSGCVAAETARILGDKFNLHGESVDAALQVLALCEQAQDGAIWQPPENFPDADDVAIIAAALAAQADLFITGDKALLALGAVEGMPILEPRAAYLRLRGLG
>AP018558.1|BBD76988.1|763349_763751_-|type-III-B-CRISPR-module-associated-protein-Cmr5
MSAHTHTHTQTHQNQHTRLTLEQQRAQDAWNKSARYTDKHRNFAKSMPALIMNSGLMQVLAFCHEKGGEHEEIAQDLRHWLNRRFNGVDKDPGFEVFMEMLLLAGPRQYQMATTEAFAWLKWLRQMASARNTR
>AP018558.1|BBD76989.1|763753_764689_-|type-III-B-CRISPR-module-RAMP-protein-Cmr4
MFQKHAALFLYAVSPVHMGAGQAIGVIDNPIQRERHTNHPCFAGSGIKGALRHGFAALSDDKSLLDRLFGPEAGKSDLHAGAVSVGDAQLVAFPVRSLKGGYVYATCPTALERARRLLSLIGVQVTWPPLPDLNEGECLLANPALTTKRTENGKQASVLHLEAFEYVVKEEKCEALESIGNWLKDNALPQQNGKDEAFEYFRSKIASDLVVLSDTDFAYFSEHATLVEPHVRINDATGTADDGGLFYTENLPPESLLVAPLLVSRTRSGKDDIAPEAVMGHIKGVIDGKLLQIGGDATTGRGLVVAKVVEG

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Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

28010 : 42956

uncultured_Mediterranean_phage(11.11%)

tRNA,protease

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
BBD76294.1\|28010_29552_-\|protease	serine protease	A0A1B1IT49	uncultured_Mediterranean_phage	2.0e-26	29.2
BBD76295.1\|29604_30591_+	transcriptional regulator, LysR family	NA	NA	NA	NA
BBD76296.1\|30641_31451_+	hypothetical protein	A4JWQ6	Burkholderia_virus	1.4e-55	48.8
BBD76297.1\|31618_32092_+	ferritin, Dps family protein	W5S6G8	Pithovirus	6.7e-13	34.0
BBD76298.1\|32151_32586_+	hypothetical protein	NA	NA	NA	NA
BBD76299.1\|32538_35052_-	DNA topoisomerase III	A0A1X9I6W8	Streptococcus_phage	1.4e-45	29.8
BBD76300.1\|35093_36239_-	DNA protecting protein DprA	S6BFL3	Thermus_phage	2.3e-22	34.6
BBD76301.1\|36235_37390_-	hypothetical protein	A0A0M4R3I1	Arthrobacter_phage	3.9e-06	53.3
BBD76302.1\|37431_37935_+	peptide deformylase	E3SLL2	Synechococcus_phage	2.4e-16	43.4
BBD76303.1\|37931_38924_+\|tRNA	methionyl-tRNA formyltransferase	E3SNR5	Prochlorococcus_phage	3.6e-08	35.3
BBD76304.1\|38913_39774_+	heat shock protein HtpX	NA	NA	NA	NA
BBD76305.1\|39804_40227_-	hypothetical protein	NA	NA	NA	NA
BBD76306.1\|40230_41928_-	hypothetical protein	NA	NA	NA	NA
BBD76307.1\|41924_42956_-	glycosyltransferase	S5FKN0	Shigella_phage	5.5e-68	44.2

DBSCAN-SWA_2

845463 : 853282

Escherichia_phage(33.33%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
BBD77061.1\|845463_846546_+	dTDP-glucose 4,6-dehydratase	A0A1D7XFE8	Escherichia_phage	4.5e-81	44.5
BBD77062.1\|846542_847487_+	dTDP-4-dehydrorhamnose reductase	NA	NA	NA	NA
BBD77063.1\|847504_848455_+	glucose-1-phosphate thymidylyltransferase	I7I009	Enterobacteria_phage	2.2e-92	55.7
BBD77064.1\|848475_849027_+	dTDP-4-dehydrorhamnose 3,5-epimerase	A0A291LA62	Escherichia_phage	1.5e-48	53.1
BBD77065.1\|849037_850417_-	peptidase family protein	G9BW84	Planktothrix_phage	7.9e-22	47.6
BBD77066.1\|850518_850722_+	cold shock-like protein	A0A2H4N7Y6	Lake_Baikal_phage	8.9e-23	78.8
BBD77067.1\|850824_850992_-	50S ribosomal protein L33	NA	NA	NA	NA
BBD77068.1\|851005_851242_-	50S ribosomal protein L28	NA	NA	NA	NA
BBD77069.1\|851308_851992_-	DNA repair protein radC	NA	NA	NA	NA
BBD77070.1\|852016_853282_+	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate synthase	Q9HH70	Methanothermobacter_phage	3.6e-37	31.8

DBSCAN-SWA_3

1021531 : 1049107

Burkholderia_phage(18.52%)

tail,plate

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
BBD77220.1\|1021531_1021888_+	hypothetical protein	R9U0S8	Rhizobium_phage	2.0e-06	33.6
BBD77221.1\|1021946_1022435_+	phage lysozyme	A0A068CDE9	Acinetobacter_phage	4.8e-22	43.0
BBD77222.1\|1022449_1023358_-	hypothetical protein	Q8LTB7	Lactobacillus_phage	2.0e-26	28.5
BBD77223.1\|1023387_1023939_+	hypothetical protein	NA	NA	NA	NA
BBD77224.1\|1023928_1024378_+	hypothetical protein	NA	NA	NA	NA
BBD77225.1\|1024367_1024691_+	hypothetical protein	NA	NA	NA	NA
BBD77226.1\|1024694_1024838_+	hypothetical protein	NA	NA	NA	NA
BBD77227.1\|1024837_1025368_+	hypothetical protein	Q6QIC2	Burkholderia_phage	1.2e-23	40.5
BBD77228.1\|1025360_1026734_+	hypothetical protein	A0A1B1P714	Rhodovulum_phage	8.0e-99	46.5
BBD77229.1\|1026727_1028329_+	hypothetical protein	A0A2P9JZI9	Alteromonadaceae_phage	3.2e-91	40.2
BBD77230.1\|1028328_1028520_+	hypothetical protein	NA	NA	NA	NA
BBD77231.1\|1028503_1029841_+	virion morphogenesis protein	M4SNU0	Rhodobacter_phage	8.9e-79	59.7
BBD77232.1\|1030158_1030644_+	virion morphogenesis protein	F8TVC6	EBPR_siphovirus	1.8e-13	36.5
BBD77233.1\|1030877_1031858_+	Mu-like prophage I protein	A0A1B0T6J6	Pelagibaca_phage	7.6e-35	33.1
BBD77234.1\|1031863_1032772_+	hypothetical protein	A0SMP3	Pseudomonas_virus	3.8e-113	66.0
BBD77235.1\|1032775_1032985_+	hypothetical protein	NA	NA	NA	NA
BBD77236.1\|1032988_1033426_+	hypothetical protein	A0A2P9JZJ4	Alteromonadaceae_phage	1.3e-18	42.3
BBD77237.1\|1033425_1033878_+	hypothetical protein	NA	NA	NA	NA
BBD77238.1\|1033879_1034071_+	hypothetical protein	NA	NA	NA	NA
BBD77239.1\|1034074_1035310_+\|tail	phage tail sheath protein	A4JWK5	Burkholderia_virus	1.4e-126	49.6
BBD77240.1\|1035320_1035842_+\|tail	phage tail tube protein	A4JWK6	Burkholderia_virus	3.9e-46	52.9
BBD77241.1\|1035845_1036094_+	hypothetical protein	Q6QIA8	Burkholderia_phage	6.0e-13	54.4
BBD77242.1\|1036207_1036375_-	hypothetical protein	NA	NA	NA	NA
BBD77243.1\|1036376_1039472_+\|tail	phage tail tape measure protein	A4JWL0	Burkholderia_virus	9.3e-87	33.2
BBD77244.1\|1039468_1040404_+	phage P2 GpU family protein	Q6QIA4	Burkholderia_phage	2.4e-22	38.0
BBD77245.1\|1040391_1040607_+\|tail	phage tail protein	R9U1D8	Rhizobium_phage	7.2e-07	42.0
BBD77246.1\|1040599_1041634_+	bacteriophage regulatory protein	A0A0C5AJ59	Bacteriophage	4.5e-22	26.1
BBD77247.1\|1041623_1042052_+\|plate	phage baseplate component	Q6QIA1	Burkholderia_phage	6.5e-23	45.2
BBD77248.1\|1042060_1042351_+	PAAR repeat-containing protein	K4ICQ1	Acidithiobacillus_phage	7.0e-21	50.0
BBD77249.1\|1042398_1042761_+\|plate	baseplate protein	A4JWL5	Burkholderia_virus	4.9e-24	52.5
BBD77250.1\|1042757_1043570_+\|plate	bacteriophage baseplate assembly protein	Q6QI99	Burkholderia_phage	2.6e-49	38.1
BBD77251.1\|1043562_1044597_+\|tail	phage tail protein	NA	NA	NA	NA
BBD77252.1\|1044593_1045031_+	hypothetical protein	A0A291L9V2	Bordetella_phage	4.9e-34	59.6
BBD77253.1\|1045043_1045985_+	hypothetical protein	R9TNJ7	Vibrio_phage	2.2e-07	56.0
BBD77254.1\|1046002_1046332_+	hypothetical protein	NA	NA	NA	NA
BBD77255.1\|1046360_1047470_+	hypothetical protein	D4HTV7	Vibrio_phage	4.5e-68	40.9
BBD77256.1\|1047736_1047883_+	hypothetical protein	NA	NA	NA	NA
BBD77257.1\|1047879_1048071_+	hypothetical protein	D4HTV9	Vibrio_phage	9.2e-06	74.3
BBD77258.1\|1048063_1049107_+	phage-encoded reverse transcriptase	A0A1I9KFG3	Aeromonas_phage	8.3e-64	41.3

DBSCAN-SWA_4

1366459 : 1373878

Indivirus(33.33%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
BBD77548.1\|1366459_1367569_+	riboflavin biosynthesis protein RibD	A0A1V0SE20	Indivirus	1.5e-39	33.4
BBD77549.1\|1367534_1367948_+	cobalamin biosynthesis protein CbiX	NA	NA	NA	NA
BBD77550.1\|1367983_1368805_-	molybdopterin biosynthesis protein MoeB	A0A1V0SCZ9	Indivirus	3.4e-12	29.9
BBD77551.1\|1368761_1369208_-	hypothetical protein	NA	NA	NA	NA
BBD77552.1\|1369251_1370709_-	peptidase S41	A0A0R6PIZ1	Moraxella_phage	7.8e-20	26.9
BBD77553.1\|1370718_1371963_-	peptidase M23	Q8SBN9	Clostridium_phage	3.9e-12	37.9
BBD77554.1\|1371971_1372718_-	phosphoglycerate mutase	NA	NA	NA	NA
BBD77555.1\|1372834_1373149_+	transcriptional regulator	NA	NA	NA	NA
BBD77556.1\|1373177_1373615_+	rhodanese	A0A2N9QVZ6	Dishui_lake_phycodnavirus	1.1e-09	31.0
BBD77557.1\|1373611_1373878_+	glutaredoxin	V9QKN6	Rhizobium_phage	1.7e-10	42.1

DBSCAN-SWA_5

1924666 : 1931275

uncultured_Mediterranean_phage(33.33%)

tRNA

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
BBD78073.1\|1924666_1925599_-	deacetylase	A0A2K9L2T7	Tupanvirus	7.2e-35	31.1
BBD78074.1\|1925648_1926680_-	RNA polymerase sigma factor RpoS	G8CLC7	Synechococcus_phage	7.2e-28	34.3
BBD78075.1\|1926714_1927638_-	peptidase	Q8SBN9	Clostridium_phage	1.7e-15	38.5
BBD78076.1\|1927648_1928332_-	protein-L-isoaspartate O-methyltransferase	A0A1B1IU40	uncultured_Mediterranean_phage	1.5e-29	46.5
BBD78077.1\|1928343_1929081_-	5'/3'-nucleotidase SurE	A0A1B1ITZ2	uncultured_Mediterranean_phage	1.3e-71	53.1
BBD78078.1\|1929135_1929882_-\|tRNA	tRNA threonylcarbamoyladenosine dehydratase	NA	NA	NA	NA
BBD78079.1\|1929851_1930808_-	MBL fold metallo-hydrolase	NA	NA	NA	NA
BBD78080.1\|1930813_1931275_-	single-stranded DNA-binding protein	A0A291LCB6	Klebsiella_phage	2.2e-40	70.5

DBSCAN-SWA_6

2079597 : 2120311

Enterobacteria_phage(30.0%)

transposase,integrase,tRNA

attL 2100692:2100709|attR 2126451:2126468

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
BBD78218.1\|2079597_2080581_+\|transposase	transposase	E5E3P6	Burkholderia_phage	5.9e-112	61.3
BBD78219.1\|2080700_2082047_-	O-antigen ligase family protein	NA	NA	NA	NA
BBD78220.1\|2082057_2082474_-	hypothetical protein	NA	NA	NA	NA
BBD78221.1\|2082486_2083212_-	Rossman fold protein, TIGR00730 family	A0A1D8KU27	Synechococcus_phage	9.6e-19	34.8
BBD78222.1\|2083288_2086207_+	DNA polymerase I	A0A142KBX8	Gordonia_phage	1.4e-55	31.0
BBD78223.1\|2086203_2088237_+	lipid A export permease/ATP-binding protein MsbA	W8CYL7	Bacillus_phage	5.4e-27	41.6
BBD78224.1\|2088371_2089019_+	hypothetical protein	NA	NA	NA	NA
BBD78225.1\|2089032_2090127_+	hypothetical protein	NA	NA	NA	NA
BBD78226.1\|2090198_2091536_-	23S rRNA (uracil(1939)-C(5))-methyltransferase	NA	NA	NA	NA
BBD78227.1\|2091535_2092798_-	hypothetical protein	NA	NA	NA	NA
BBD78228.1\|2092821_2093712_-	4-hydroxy-tetrahydrodipicolinate synthase	NA	NA	NA	NA
BBD78229.1\|2093754_2094936_-	cardiolipin synthase B	NA	NA	NA	NA
BBD78230.1\|2094946_2095693_-	EEP domain-containing protein	NA	NA	NA	NA
BBD78231.1\|2095707_2096859_-	quinolinate synthetase	NA	NA	NA	NA
BBD78232.1\|2096876_2097329_-	rhodanese	NA	NA	NA	NA
BBD78233.1\|2097343_2098615_+	cation transporter	NA	NA	NA	NA
BBD78234.1\|2098754_2100164_+	glutamine synthetase	NA	NA	NA	NA
BBD78235.1\|2100235_2100622_+	DUF4124 domain-containing protein	NA	NA	NA	NA
BBD78236.1\|2100626_2101676_+	PAS domain-containing sensor histidine kinase	Q8QKV7	Ectocarpus_siliculosus_virus	1.2e-06	27.2
2100692:2100709	attL	NA	NA	NA	NA
BBD78237.1\|2101672_2103316_+	nitrogen regulation protein NR(I)	NA	NA	NA	NA
BBD78238.1\|2103363_2103633_+	hypothetical protein	NA	NA	NA	NA
BBD78239.1\|2103625_2103883_+	hypothetical protein	NA	NA	NA	NA
BBD78240.1\|2103932_2104367_+	RNA polymerase-binding protein DksA	NA	NA	NA	NA
BBD78241.1\|2104451_2106218_+\|tRNA	cysteine--tRNA ligase	A0A0U2SJ70	Niemeyer_virus	5.7e-41	34.0
BBD78242.1\|2106671_2107352_+	hypothetical protein	NA	NA	NA	NA
BBD78243.1\|2107351_2110861_+	hypothetical protein	NA	NA	NA	NA
BBD78244.1\|2111174_2113229_+	sugar transporter	NA	NA	NA	NA
BBD78245.1\|2113242_2114406_+	lipopolysaccharide biosynthesis protein	NA	NA	NA	NA
BBD78246.1\|2114409_2115513_-	glycosyltransferase	NA	NA	NA	NA
BBD78247.1\|2115528_2116068_-	glycosyltransferase	NA	NA	NA	NA
BBD78248.1\|2116209_2117193_-\|transposase	transposase	E5E3P6	Burkholderia_phage	3.4e-112	61.3
BBD78249.1\|2117625_2118327_+\|transposase	transposase	U5N3F9	Enterobacteria_phage	2.8e-68	58.6
BBD78250.1\|2118349_2118769_+\|transposase	transposase	U5N3F9	Enterobacteria_phage	2.9e-28	50.0
BBD78251.1\|2118874_2119405_+	AAA family ATPase	U5N3V8	Enterobacteria_phage	8.2e-68	78.2
BBD78252.1\|2119413_2119689_-\|integrase	integrase, partial	NA	NA	NA	NA
BBD78253.1\|2119789_2120311_-\|transposase	transposase, partial	NA	NA	NA	NA
2126451:2126468	attR	NA	NA	NA	NA

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

Overview of predicted results

Overview of the results

Cas Category Instructions

Results visualization

1. AP018559

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection

2. AP018558

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Click the colored protein region to show detailed information

Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection