CRISPRimmunity

Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Self targeting spacer number	Target MGE spacer number	Prophage number	Anti-CRISPR protein number
AP018786	Sutterella megalosphaeroides DNA, complete genome	2 crisprs	cas10,csm2gr11,csm3gr7,csm4gr5,csm5gr7,cas6,csx16,csx1,cas14j,WYL,csa3,Cas14u_CAS-V,cas2,cas1,cas5,cas7,cas6e,cse2gr11,cas8e,cas3,DinG,DEDDh	0	9	1	0

1. AP018786

CRISPR-Cas detection and classification

Crispr_ID: AP018786_1

• CRISPR_ID: AP018786_1
• CRISPR_location: 1149200-1149440
• CRISPR_type: TypeIII
• Repeat_type: NA
• Spacer_info: 3 spacers
• Cas_protein_info: cas6,csm5gr7,csm4gr5,csm3gr7,csm2gr11,cas10,csx16,csx1

Consensus repeat of AP018786_1

Consensus_repeat	Method
ATTCTGACGGTACCCTGATCTTGAAGGGATTGAGAC	PILER-CR
ATTCTGACGGTACCCTGATCTTGAAGGGATTGAGAC	CRISPRCasFinder
ATTCTGACGGTACCCTGATCTTGAAGGGATTGAGAC	CRT

spacers of AP018786_1

>1.1|1149236|32|AP018786|PILER-CR,CRISPRCasFinder,CRT
ACGTAGATCCGCTTGACTTCGGTAAAAAGCTC
>1.2|1149304|33|AP018786|PILER-CR,CRISPRCasFinder,CRT
TGATTACCCGAACCAGGCTGATTGCCGGGTTGA
>1.3|1149373|32|AP018786|PILER-CR,CRISPRCasFinder,CRT
AGAGCACACATGCAGATGTCGTTGAGGATCGT

CRISPR arrays and Neighbor proteins around AP018786_1

CRISPR arrays

The CRISPR arrays of AP018786_1

>merge|AP018786|1|1149200-1149440|PILER-CR,CRISPRCasFinder,CRT
ATTCTGACGGTACCCTGATCTTGAAGGGATTGAGACACGTAGATCCGCTTGACTTCGGTAAAAAGCTCATTCTGACGGTACCCTGATCTTGAAGGGATTGAGACTGATTACCCGAACCAGGCTGATTGCCGGGTTGAATTCTGACGGTACCCTGATCTTGAAGGGATTGAGACAGAGCACACATGCAGATGTCGTTGAGGATCGTATTCTGACGGTACCCTGATCTTGAAGGGATTGAGAC

>AP018786|1|1|1149200-1149440|PILER-CR
ATTCTGACGGTACCCTGATCTTGAAGGGATTGAGAC	ACGTAGATCCGCTTGACTTCGGTAAAAAGCTC
ATTCTGACGGTACCCTGATCTTGAAGGGATTGAGAC	TGATTACCCGAACCAGGCTGATTGCCGGGTTGA
ATTCTGACGGTACCCTGATCTTGAAGGGATTGAGAC	AGAGCACACATGCAGATGTCGTTGAGGATCGT
ATTCTGACGGTACCCTGATCTTGAAGGGATTGAGAC

>AP018786|1|1|1149200-1149440|CRISPRCasFinder
ATTCTGACGGTACCCTGATCTTGAAGGGATTGAGAC	ACGTAGATCCGCTTGACTTCGGTAAAAAGCTC
ATTCTGACGGTACCCTGATCTTGAAGGGATTGAGAC	TGATTACCCGAACCAGGCTGATTGCCGGGTTGA
ATTCTGACGGTACCCTGATCTTGAAGGGATTGAGAC	AGAGCACACATGCAGATGTCGTTGAGGATCGT
ATTCTGACGGTACCCTGATCTTGAAGGGATTGAGAC

>AP018786|1|1|1149200-1149440|CRT
ATTCTGACGGTACCCTGATCTTGAAGGGATTGAGAC	ACGTAGATCCGCTTGACTTCGGTAAAAAGCTC
ATTCTGACGGTACCCTGATCTTGAAGGGATTGAGAC	TGATTACCCGAACCAGGCTGATTGCCGGGTTGA
ATTCTGACGGTACCCTGATCTTGAAGGGATTGAGAC	AGAGCACACATGCAGATGTCGTTGAGGATCGT
ATTCTGACGGTACCCTGATCTTGAAGGGATTGAGAC

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
AP018786.1|BBF23046.1|1149443_1149656_+|hypothetical-protein	unknown	unknown	unknown
AP018786.1|BBF23050.1|1153880_1154498_+|hypothetical-protein	unknown	unknown	gnl|CDD|379228
AP018786.1|BBF23044.1|1147043_1148162_+|hypothetical-protein	csm5gr7	mkCas0182_csm5gr7_CAS-III-A	gnl|CDD|224251
AP018786.1|BBF23041.1|1145003_1145708_+|type-III-A-CRISPR-associated-RAMP-protein-Csm3	csm3gr7	cd09684_csm3gr7_CAS-III-A:CAS-III-D	gnl|CDD|187815
AP018786.1|BBF23048.1|1151542_1152709_+|hypothetical-protein	csx1	pfam09002_csx1_CAS-III	gnl|CDD|312526
AP018786.1|BBF23049.1|1152705_1153869_+|hypothetical-protein	csx1	pfam09623_csx1_CAS-III	gnl|CDD|187872
AP018786.1|BBF23036.1|1138440_1139493_+|hypothetical-protein	unknown	unknown	gnl|CDD|350237
AP018786.1|BBF23051.1|1154702_1155695_+|ribokinase	unknown	unknown	gnl|CDD|238579
AP018786.1|BBF23045.1|1148171_1149101_+|CRISPR-associated-protein-Cas6	cas6	mkCas0066_cas6_CAS-I:CAS-III	gnl|CDD|187890
AP018786.1|BBF23047.1|1151173_1151473_+|hypothetical-protein	csx16	pfam09652_csx16_CAS-I:CAS-III	gnl|CDD|370601
AP018786.1|BBF23039.1|1141825_1144447_+|type-III-A-CRISPR-associated-protein-Cas10/Csm1	cas10	cd09680_cas10_CAS-III	gnl|CDD|187811
AP018786.1|BBF23038.1|1140647_1141667_+|hypothetical-protein	unknown	unknown	unknown
AP018786.1|BBF23043.1|1146696_1147032_+|hypothetical-protein	unknown	unknown	gnl|CDD|182930
AP018786.1|BBF23042.1|1145748_1146687_+|CRISPR-associated-protein,-Csm4-family	csm4gr5	mkCas0089_csm4gr5_CAS-III-A:CAS-III-D	unknown
AP018786.1|BBF23055.1|1160726_1160963_-|iron-transporter-FeoA	unknown	unknown	gnl|CDD|377199
AP018786.1|BBF23054.1|1158229_1160557_-|ferrous-iron-transport-protein-B	unknown	unknown	gnl|CDD|236563
AP018786.1|BBF23052.1|1155757_1156726_+|ribosylpyrimidine-nucleosidase	unknown	unknown	gnl|CDD|239117
AP018786.1|BBF23053.1|1156979_1158104_+|5-methyltetrahydropteroyltriglutamate---homocystei-ne-S-methyltransferase	unknown	unknown	gnl|CDD|180601
AP018786.1|BBF23040.1|1144491_1144923_+|type-III-A-CRISPR-associated-protein-Csm2	csm2gr11	cd09647_csm2gr11_CAS-III-A	gnl|CDD|367635
AP018786.1|BBF23037.1|1139610_1140648_+|hypothetical-protein	unknown	unknown	gnl|CDD|130711

HMMScan for Signature genes

Protein	Cas_name	Cas_description	E-value	Identity	Coverage
AP018786.1|BBF23045.1|1148171_1149101_+|CRISPR-associated-protein-Cas6	cas6	mkCas0066_cas6_CAS-I:CAS-III	2.7e-98	323.8	0.1
AP018786.1|BBF23047.1|1151173_1151473_+|hypothetical-protein	csx16	pfam09652_csx16_CAS-I:CAS-III	3.7e-29	95.6	0.0
AP018786.1|BBF23039.1|1141825_1144447_+|type-III-A-CRISPR-associated-protein-Cas10/Csm1	cas10	cd09680_cas10_CAS-III	5.2e-115	380.1	0.0
AP018786.1|BBF23044.1|1147043_1148162_+|hypothetical-protein	csm5gr7	mkCas0182_csm5gr7_CAS-III-A	4.3e-11	37.1	0.0
AP018786.1|BBF23042.1|1145748_1146687_+|CRISPR-associated-protein,-Csm4-family	csm4gr5	mkCas0089_csm4gr5_CAS-III-A:CAS-III-D	5.5e-85	280.1	0.0
AP018786.1|BBF23048.1|1151542_1152709_+|hypothetical-protein	csx1	pfam09002_csx1_CAS-III	2.3e-53	175.9	0.0
AP018786.1|BBF23049.1|1152705_1153869_+|hypothetical-protein	csx1	pfam09623_csx1_CAS-III	3.4e-77	253.8	0.0
AP018786.1|BBF23040.1|1144491_1144923_+|type-III-A-CRISPR-associated-protein-Csm2	csm2gr11	cd09647_csm2gr11_CAS-III-A	8.2e-17	56.7	0.2
AP018786.1|BBF23041.1|1145003_1145708_+|type-III-A-CRISPR-associated-RAMP-protein-Csm3	csm3gr7	cd09684_csm3gr7_CAS-III-A:CAS-III-D	5.6e-64	210.5	0.0

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
AP018786.1|BBF23051.1|1154702_1155695_+|ribokinase	gnl|CDD|238579	cd01174, ribokinase, Ribokinase catalyses the phosphorylation of ribose to ribose-5-phosphate using ATP. This reaction is the first step in the ribose metabolism. It traps ribose within the cell after uptake and also prepares the sugar for use in the synthesis of nucleotides and histidine, and for entry into the pentose phosphate pathway. Ribokinase is dimeric in solution.	6.14324e-76
AP018786.1|BBF23050.1|1153880_1154498_+|hypothetical-protein	gnl|CDD|379228	pfam13508, Acetyltransf_7, Acetyltransferase (GNAT) domain. This domain catalyzes N-acetyltransferase reactions.	5.89703e-06
AP018786.1|BBF23044.1|1147043_1148162_+|hypothetical-protein	gnl|CDD|224251	COG1332, COG1332, CRISPR system related protein, RAMP superfamily [Defense mechanisms].	0.00196552
AP018786.1|BBF23041.1|1145003_1145708_+|type-III-A-CRISPR-associated-RAMP-protein-Csm3	gnl|CDD|187815	cd09684, Csm3_III-A, CRISPR/Cas system-associated RAMP superfamily protein Csm3. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and associated Cas proteins comprise a system for heritable host defense by prokaryotic cells against phage and other foreign DNA; RAMP superfamily protein.	3.65099e-70
AP018786.1|BBF23043.1|1146696_1147032_+|hypothetical-protein	gnl|CDD|182930	PRK11054, helD, DNA helicase IV; Provisional.	0.00354631
AP018786.1|BBF23048.1|1151542_1152709_+|hypothetical-protein	gnl|CDD|312526	pfam09002, DUF1887, Domain of unknown function (DUF1887). This domain is found in a set of hypothetical bacterial proteins.	1.11884e-39
AP018786.1|BBF23049.1|1152705_1153869_+|hypothetical-protein	gnl|CDD|187872	cd09741, Csx1_III-U, CRISPR/Cas system-associated protein Csx1. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and associated Cas proteins comprise a system for heritable host defense by prokaryotic cells against phage and other foreign DNA; Protein of this family often fused to HTH domain; Some proteins could have an additional fusion with RecB-family nuclease domain; Core domain appears to have a Rossmann-like fold; loosely associated with CRISPR/Cas systems; also known as NE0113 family.	3.52207e-80
AP018786.1|BBF23036.1|1138440_1139493_+|hypothetical-protein	gnl|CDD|350237	cd11297, PIN_LabA-like_N_1, uncharacterized subfamily of N-terminal LabA-like PIN domains. This N-terminal LabA-like PIN domain is found in a well conserved group of mainly bacterial proteins with no defined function, which contain a C-terminal LabA_like_C domain. LabA from Synechococcus elongatus PCC 7942, (which does not contain this C-terminal domain), has been shown to play a role in cyanobacterial circadian timing. The LabA-like C-terminal domains characteristic of this subfamily may be related to the LOTUS domain family (which also co-occurs with LabA-like N-terminal domains). The function of the N-terminal domain is unknown. The LabA-like PIN domain family also includes the N-terminal domain of limkain b1, a human autoantigen localized to a subset of ABCD3 and PXF marked peroxisomes. Other members are the LabA-like PIN domains of human ZNF451, uncharacterized Bacillus subtilis YqxD and Escherichia coli YaiI. Curiously, a gene labeled NicB from Pseudomonas putida S16, which is described as a putative NADH-dependent hydroxylase involved in the microbial degradation of nicotine also falls into the LabA-like PIN family. The PIN (PilT N terminus) domain belongs to a large nuclease superfamily. The structural properties of the PIN domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions; in some members, additional metal coordinating residues can be found while some others lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.	1.31439e-42
AP018786.1|BBF23054.1|1158229_1160557_-|ferrous-iron-transport-protein-B	gnl|CDD|236563	PRK09554, feoB, Fe(2+) transporter permease subunit FeoB.	0
AP018786.1|BBF23045.1|1148171_1149101_+|CRISPR-associated-protein-Cas6	gnl|CDD|187890	cd09760, Cas6_III, CRISPR/Cas system-associated RAMP superfamily protein Cas6. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and associated Cas proteins comprise a system for heritable host defense by prokaryotic cells against phage and other foreign DNA; Cas6 is an endoribonuclease that generates crRNAs, predicted subunit of Cascade complex.	2.41263e-09
AP018786.1|BBF23039.1|1141825_1144447_+|type-III-A-CRISPR-associated-protein-Cas10/Csm1	gnl|CDD|187811	cd09680, Cas10_III, CRISPR/Cas system-associated protein Cas10. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and associated Cas proteins comprise a system for heritable host defense by prokaryotic cells against phage and other foreign DNA; Multidomain protein with permuted HD nuclease domain, palm domain and Zn-ribbon; signature gene for type III; also known as Csm1 family.	3.02253e-115
AP018786.1|BBF23047.1|1151173_1151473_+|hypothetical-protein	gnl|CDD|370601	pfam09652, Cas_VVA1548, Putative CRISPR-associated protein (Cas_VVA1548). This entry represents a conserved region of about 95 amino acids found exclusively in species with CRISPRs (Clustered Regularly Interspaced Short Palindromic Repeats). In all bacterial species that contain this entry, the genes encoding the proteins are in the midst of a cluster of cas (CRISPR-associated) genes.	2.76446e-33
AP018786.1|BBF23055.1|1160726_1160963_-|iron-transporter-FeoA	gnl|CDD|377199	pfam04023, FeoA, FeoA domain. This family includes FeoA a small protein, probably involved in Fe2+ transport. This presumed short domain is also found at the C-terminus of a variety of metal dependent transcriptional regulators. This suggests that this domain may be metal-binding. In most cases this is likely to be either iron or manganese.	2.29655e-19
AP018786.1|BBF23052.1|1155757_1156726_+|ribosylpyrimidine-nucleosidase	gnl|CDD|239117	cd02651, nuc_hydro_IU_UC_XIUA, nuc_hydro_IU_UC_XIUA: inosine-uridine preferring, xanthosine-inosine-uridine-adenosine-preferring and, uridine-cytidine preferring nucleoside hydrolases. Nucleoside hydrolases cleave the N-glycosidic bond in nucleosides generating ribose and the respective base. These enzymes vary in their substrate specificity. This group contains proteins similar to nucleoside hydrolases which hydrolyze both pyrimidine and purine ribonucleosides: the inosine-uridine preferring nucleoside hydrolase from Crithidia fasciculata, the inosine-uridine-xanthosine preferring nucleoside hydrolase RihC from Escherichia coli and the xanthosine-inosine-uridine-adenosine-preferring nucleoside hydrolase RihC from Salmonella enterica serovar Typhimurium. This group also contains proteins similar to the pyrimidine-specific uridine-cytidine preferring nucleoside hydrolases URH1 from Saccharomyces cerevisiae, E. coli RihA and E. coli RihB. E. coli RihA is equally efficient with uridine and cytidine, E. coli RihB prefers cytidine over uridine. S. cerevisiae URH1 prefers uridine over cytidine. .	3.32366e-135
AP018786.1|BBF23053.1|1156979_1158104_+|5-methyltetrahydropteroyltriglutamate---homocystei-ne-S-methyltransferase	gnl|CDD|180601	PRK06520, PRK06520, 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase.	1.5356e-169
AP018786.1|BBF23040.1|1144491_1144923_+|type-III-A-CRISPR-associated-protein-Csm2	gnl|CDD|367635	pfam03750, Csm2_III-A, Csm2 Type III-A. Clusters of short DNA repeats with non-homologous spacers, which are found at regular intervals in the genomes of phylogenetically distinct prokaryotic species, comprise a family with recognisable features. This family is known as CRISPR (short for Clustered Regularly Interspaced Short Palindromic Repeats). A number of protein families appear only in association with these repeats and are designated Cas (CRISPR-associated) proteins. This entry represents Csm2 Type III-A, a family of Cas proteins also known as TM1810/Csm2.	1.98368e-19
AP018786.1|BBF23037.1|1139610_1140648_+|hypothetical-protein	gnl|CDD|130711	TIGR01650, PD_CobS, cobaltochelatase, CobS subunit. This model describes Pseudomonas denitrificans CobS gene product, which is a cobalt chelatase subunit that functions in cobalamin biosynthesis. Cobalamin (vitamin B12) can be synthesized via several pathways, including an aerobic pathway (found in Pseudomonas denitrificans) and an anaerobic pathway (found in P. shermanii and Salmonella typhimurium). These pathways differ in the point of cobalt insertion during corrin ring formation. There are apparently a number of variations on these two pathways, where the major differences seem to be concerned with the process of ring contraction. Confusion regarding the functions of enzymes found in the aerobic vs. anaerobic pathways has arisen because nonhomologous genes in these different pathways were given the same gene symbols. Thus, cobS in the aerobic pathway (P. denitrificans) is not a homolog of cobS in the anaerobic pathway (S. typhimurium). It should be noted that E. coli synthesizes cobalamin only when it is supplied with the precursor cobinamide, which is a complex intermediate. Additionally, all E. coli cobalamin synthesis genes (cobU, cobS and cobT) were named after their Salmonella typhimurium homologs which function in the anaerobic cobalamin synthesis pathway. This model describes the aerobic cobalamin pathway Pseudomonas denitrificans CobS gene product, which is a cobalt chelatase subunit, with a MW ~37 kDa. The aerobic pathway cobalt chelatase is a heterotrimeric, ATP-dependent enzyme that catalyzes cobalt insertion during cobalamin biosynthesis. The other two subunits are the P. denitrificans CobT (TIGR01651) and CobN (pfam02514 CobN/Magnesium Chelatase) proteins. To avoid potential confusion with the nonhomologous Salmonella typhimurium/E.coli cobS gene product, the P. denitrificans gene symbol is not used in the name of this model. [Biosynthesis of cofactors, prosthetic groups, and carriers, Heme, porphyrin, and cobalamin].	4.52376e-41

Protein sequences

>AP018786.1|BBF23045.1|1148171_1149101_+|CRISPR-associated-protein-Cas6
MTDIPLTWPLARYKFVFAVTSPIELPDYAGSAFRGAFGRALRKTACMTRMSDCSTCPLRSTCAYTSVFETPAPAEHALQSFSKVPNPYVIEAPLGGRKHLDVGDRLEFSVVLFGDALQKLALIVYAMQRAFSYEVCRGKASLQCVYLETEAGDEPIYEPGAESIKPHAQQLTLTAPRGEADKTLRFVTPLRLQNNGKIYGAKDIEPLPFLTALVRRIGLLAEFHGDKPNLNYHALREAALAVESSHALSWQNWKRYSSRQQKSMYLGGLVGTWTLSRVPEALQIFLELGSYLHVGKNATFGLGRYEILH
>AP018786.1|BBF23044.1|1147043_1148162_+|hypothetical-protein
MYTAAGDDDTAFIPGSSVKGAVHTALLERLHIGKRHVCEDDDLWGKGFEKRPLRLLKVGDFMPEAPVVMRAVSAKRLAKDARGTSGRKEGIPMAIETLWPGGYRAFSSSWTIEGDRSESGGADAYVDFQRIARDLTAFNRPKLETELRLLDVDPRAGDWVRRMREILGSIDPLLKRGDMALLRVGKFQGALSLRLSASDEKPPKMQTFVVNDSQVLPFGWALLEFRDEVSEPLKAWCRAWPDMQTVDLQALRQARREEETRRREEARAEVERRKAAEVAEAAEEARLAAMSDEKRRVVVLEKSLAKYSGTVNPGSDLFRAVQVLLREAATWANIEDRKFCALTLAPLVKERGMYQGKAKKELKELLNKLAGD
>AP018786.1|BBF23043.1|1146696_1147032_+|hypothetical-protein
MHATPYLTRRVFLTLLSPVHVGCGETYDPTNYVIDEGILYGFEPSLVWLPEQERQELRRLALKGDRIGWAVFFIATVESSPLKPERPFGPGARSARTIKSSSNEREATISV
>AP018786.1|BBF23042.1|1145748_1146687_+|CRISPR-associated-protein,-Csm4-family
MQRLLITIEPRSGFVSPLKGGQLFGQLAWTIAEAFGSARLTSLLDGYVDGRPFLVLSDAMPSGVVPMPAMPVHWWAPEVNARERKRIKVLRWLPREALREPVARWRDRALTDDDLAKSGFSARSGERTRNTISRATGTTGEGRFAPFDTTDTTFGVGARLDIHAVLDETRLDRKEFLMLLENSGLFGFGADASSGAGKFVVTHAEVQPDEPPSTHFLSLAAMSPVAGVFRSDRTFYKPVTYFGRHGNMRALSSVPFKKPLLLADTGALLTTRAEAPQSLRFAGRGIAGHSAYADTVAQGYAPLLAVTPDIRH
>AP018786.1|BBF23041.1|1145003_1145708_+|type-III-A-CRISPR-associated-RAMP-protein-Csm3
MQLKNLHKITATVVCETGLHVGGNDGEMHIGGIDNPVIKHPITNEPYIPGSSLKGKMRAMLEWRSGFVKEKPLDWQDYKQSGSEAVLDILKLFGMSAGADLTVDEASAVGPTRASFWDLALDPAWVREQHEARRLLTEAKSENVINRIKGTAEHPRQTERVPSGARFSMTVTLKDLGDDERLMKTLLAGLKLVEFDGIGGSLSRGYGKVRFDDLKIDGHDATELYRSIDPFRPL
>AP018786.1|BBF23040.1|1144491_1144923_+|type-III-A-CRISPR-associated-protein-Csm2
MSYSNTNTSSSPVEWTVTLKLDPPLAGDLFSTQAERAARKCRSSKTENKSTQIRRFYDELVLWHEQVKTAPDPKAKFREIEPYLQMLRAKAAYARARKLIDENFRVLVNALLDGIKDPASLENGKLFFEAFLGFRKFLEMNEK
>AP018786.1|BBF23039.1|1141825_1144447_+|type-III-A-CRISPR-associated-protein-Cas10/Csm1
MHLIDEAARVAFASLIHDAGKFAQRAGLTVSPLTKDTHVQLYCPRTPENRPTHIHAAYTPMAIDAMEAWLPDLVGGRTAPFEGAGALEHADSLINAAGAHHRPETLLQWIVATADRAASGFEREMKTEEEARSSDGDDYIRTRLWSLLEEIRLDEPEGSAPLTRKPLRALSVDALFPRQGIVPEKRTEGEREYRALWDDFLKAGRRIPNAMRRNWPLWLDAFDTLWLVYTQAIPSATAFNARPDVSLYDHSKATAAVATALWAWCEASGRDADALVRGLKDRSLWDEESLLLVQGDFFGIQDFIFSGAASTQKKAAKILRGRSFYVSLLTEAAALAVLEKLALPATSQILNAAGKFLIVAPNTPEVRERLREAESTFEAWFVKETCAQAGLGLAFQAATLRDLTGERFPKLMRSIFESLERSKLTRYRLFDEDPTRVPGPVLEADYRFGPCAWQGRWPADALDENGLPTARLTRDEIAVGEAIVRMDTLALVDEDVRLDGLGFETKVLNGVIFGSRAVFFAHGNVDAFLRAVPAENIRRIWDFSMPRASDEVLWRGLSRRSINGYVPRLDDALVPEDEPRFAGLSSEDIGHERILPFEWLARLGQSFDERGRICGVPALCALKGDVDQLGRIFQEGLVGAGGRGMNFAKMASLSREFNAFFSIAVPFECSKAFPHVYTVFAGGDDFFFIGPLLETQRFATALRDRFVEFAAANPEVHFSAGFAVVKPGMPVRRFSEAAEDALSRAKNSGRNAACAYGECVSWTEWKALLAVQERLESWRSAYGLSTSYLYALFEIMELAERRDDPRAAIWRSRLYYRTTRWMESVARERRLSSSAASEAATQLLGSLTRDLETYGARLRIPLTNLFYGVRRAR
>AP018786.1|BBF23038.1|1140647_1141667_+|hypothetical-protein
MDVRDVFPSGVYRGKTFADLCATTSGLQYLERWVEERDKTPAARAFEDAARIALSGSGNRSVETLWFVLDATAELSRTRFGTERRGEIRAALLSARTAIAHAGAVEGSALAVLGGSGGESGDFYVLAAKKSMRKLCAIVSEAVRSRQIETHRLAADRVTILARLADWEIPEKRPEGLRALLAAFDRLLDIETADDVPTVTDLPAGPGPLATAKAAKATNTTKATNVTGASKTSERPEPVSKTASLFEAVQPSEAVSKAGFEASPATEDSETLETESPEFPRSRENRETWKETVLETGKAPARAAISTGTHTSARKTSAPTVKDATETRPLRSWEIWGGW
>AP018786.1|BBF23037.1|1139610_1140648_+|hypothetical-protein
MTETPRPDSPDGPALRSEAERGSSGFATQAYLHEVFGLRGNLARSTCGRRPIPITVCRPAAEDFDLVPDVREDYVWNVQELKYVLSAVEMGIPVYIWGHKGVGKTEMVEQICARTRRPLVRVQHTSNTEESQIVGQWTVKDGSTVFEYGPLPTAMKYGWAYLADEYDFGLPNVLGVYQAMLEGKPLMIKEAPADMRVVRPHPDFRFFATGNTNGTGDETGLYQGTVLQNAANFDRFGLVLQKGYMARKDEIKILERSTVVTNEEAGRLVDFAAQVRKAFDAGKISDTISVRTLIFAARLGVAHASFRAGLQLAFLNKLSATDRAACDALAMRIFGTNESRPGEDM
>AP018786.1|BBF23036.1|1138440_1139493_+|hypothetical-protein
MREEPSRLRADARPGTRTALLVDAENVSPKALDFVVERLGDAWDAVEVRRVYGDWRKTALSSGWHAVCLRQGLREMQQLSAVAGKNATDFAIVIDAIELAADGFGEIVVVSSDSDFMPLAQRLRERGVRYVGFGKTQTPIGYRKSCAVFTEFPEEAQAAEAAQATQAAQAPAAPKAAKVPKPSKVCQAPGASASFGKPEAPEKPEIPATATNKADEPATLGVTPVTAEDDVRLLKAINQRFHDADGFTCGGHVGHYAKMLGFHWGKRTQSRRLAAIVRRHPKVFEWRIENEHFYFRIRPEPEPKVAKFRVAALPALPAPRPRLALPRPALRRLLPAPERVPLLPLLPFTP
>AP018786.1|BBF23046.1|1149443_1149656_+|hypothetical-protein
MDMTDMTGKDVMLTLEEMAGISQVCEFYGGGIGGVYFPCEYVRMEVMCLACCIWFAMTWLTPKSYDVFTK
>AP018786.1|BBF23047.1|1151173_1151473_+|hypothetical-protein
MALWFVSRHLGARAWARARSLRVDHWVEHLDVEDVSEGDVVVGTLPVDKIAEINARGARFLALCLDLTSEMRGRELTPEDMEMLGGRLVEYRAQRVEST
>AP018786.1|BBF23048.1|1151542_1152709_+|hypothetical-protein
MTILSERVDTHIMFVSEHNSPNLWPALDPKIAPSHAILVTTPEMKAKAESLERTLRWAVPGIAIEHVDVPDGYDILMLADTLENMIRDLKDRGLRPLFNLTCGTKAMTLAAMTATETTKTPCLYLPIDSHDVIFVRELETMRLDCAMQLKHYVSLYGYVMREEPKTLPFTQELRDLAEELARTESFRQTYPAVNRLASEAKDKLAVSLDLLGPRSAAFDALLDRLEAAQLLTLNGRTVRFGNEAKRFFVNGGWLEDWAANAARRAFPGAKVVENARIDYIEGQNGKIDVKGLQNELDVVLWQNDRLVILECKTSNLGDEKRFEDVTYKLSALGKLFGKTVVPVIVSYLPLTEAAVNRAKTLRIEIVAGKDVARLEERLKGLLSEKAKA
>AP018786.1|BBF23049.1|1152705_1153869_+|hypothetical-protein
MMSERYPRRILLAVTGMSTQVVTETLWALLAKGELPTEVRLVTTEHGRNRAVRDLLDPKDGRFHAFCRDYGLEGKIAFSEDSIRVIESASGDRLTDIRTPEDNARAADVILKVVRSLCADERARVHVSIAGGRKTMGFFAGYALSLFGREQDRLSHVLVSEPFESNRDFFYPSPVSRNILSADGRLLDEAEARVTLAEIPFVRLREGLSANFPEDCTRYEDVVERAQAEMLPPVSLRIELREGAEPVLLCSGRRVNLAPTPMAVYVWLAERRKRSADGVRPGLDAAAEILALHSELFPRRSGDHARSLRALKHEEDVLPYFQEKRSLIHRALREALPERLAESYFVAALGRRPNTVYRLNVDPDAIEIVRIPDARSTEKHRAQNPPR
>AP018786.1|BBF23050.1|1153880_1154498_+|hypothetical-protein
MENVKTASTETFVRPPVFTPLASETLRAALESLRCASDVPGVPDDATDRLSALARLACEADEPEHALTLVTSEARGLLLKDASGNGLFSGFILWGPVTKETRAWLAPQGLAADFEVLLIAVEAARRRRGIGRQLLEAALARESAGSRWVLGTGNAPATERFYRAAAWEPAGLEPGYFERAYGHAVFDGNTRLTARSYFMKTAPQA
>AP018786.1|BBF23051.1|1154702_1155695_+|ribokinase
MPRVLNIGSMNIDYVYAVPHFVQPGETLAATGRALHEGGKGLNQSVAMARAGLEVEHAGLLGIDGVFLKDFLADQGVKVDAVGVSDTFPSGHTIIQVSPDGENSILYFPGTNRMLAEARIDELLSGLSKGDFVVLQNEVNDVALWVEKALARDLRVVLNPAPYTAEIARLPLNRLHALIVNRTEGRGLLEDFTKSEAVDNGETILGALADRFIRDEPASGPVLILTEGSEGVSFKIPGEAPRHFPVFPVTAVDTTGAGDTFAGYAVKALIEGVEKGKEALLEGIARATLAAALSVTKHGAAASIPTADEVARERLARIERGEWPVQGFQG
>AP018786.1|BBF23052.1|1155757_1156726_+|ribosylpyrimidine-nucleosidase
MTEVTKIILDCDPGYDDAVALLLAAGDPRIELLAVTTVAGNQSIEKVTGNARRILRAVGREDIPVAAGAIRPLVRGGAAFCPEIHGESGLDGTTLPEATTPVDPRAAAQLIVDTVMREPEGTVTLVPTGPLTNIALAARLEPRIVGRVKEVVLMGGGCKIGNVKPFAEFNIEFDPEAAYVVFNEAWKVTMVGLDLTYQARADEAVRARARAIATKPGQLLADILDAFARNYKKKSHFDAPPVHDPCAVAAVIDRSIVKCVPVPIDVELRGALTYGMTVADFRRPAPEGCRTFAAVELDQPRFWDMVIEAVERIGDPLKDVEY
>AP018786.1|BBF23053.1|1156979_1158104_+|5-methyltetrahydropteroyltriglutamate---homocystei-ne-S-methyltransferase
MERILPPYRADVVGSFLRPKCLLEARAAFEAGKLSRDELSAVEEREIAALIRKERENGLTAVTDGEFPRAFWHLDFLAGLSGIRHVKAEAWSVHFEGHQPKAETVVIEDRIGFPKDHPFLAAFDRVKRLAGDAPVKFTIPSLSMLHLICCVREANYKPIAAYAENPDLLFEDLAAAWTDAMLALYEHGCRYLQFDDTSWGEFCSAEKRAAYAARGIDVDAVGRNYVKALNAVLAKKPADLTVTMHICRGNFRSTWFSSGGYEPVAEILFANCNVDAFFLEYDSDRAGSFEPLRHIKDQTVVLGLITSKFPELEDPEAVKARIREASQYVPLERLCLSPQCGFSSTEEGNIMTEEEEWAKVRLVKQIADEVWGNN
>AP018786.1|BBF23054.1|1158229_1160557_-|ferrous-iron-transport-protein-B
MQTRTVCVVGNPNCGKTTLFNALTGSHQEVGNWPGVTVDKKVGRFTHRAVAIDLVDLPGIYSITPSGASGEDERIARDYILTGEAELVVNIVDASNLERNLYLTTQLIEMQVPMFVVVNMLDIAKQHRLSIDLDALSRELGCPVVGVVASREDGIEALKDAILAALEHPVVPHVKTHFDERIEHAVAEAAAYFRDAKVARPEWFAMQFMEEAAGLDDKLPGVDLEPVKARVRALNDETEGDTDILVASARYAFVQEVADRAITRVGEVTQSLTEKIDRVVLNRWLGIPIFLLVMYVMFLFTQNVGGAFIDFFDILVGGVMVEGFGELLTSLGAPDWLRVFLANGVGGGLQTVSTFIPVVFFLYLFLAVLEDSGYMARAAFVMDRLMRALGLPGKAFVPLIVGFGCNVPAIMATRTMDRASDRIITVMMAPFMSCGARLPVYVLFATAFFPTNGQNLVFALYVIGIVAAILTGFLLKNTVLPGSAAHFVMEIPPYHIPTLKGVMLRTWERVSGFTVRAGQAIIVIVACLTFLNSMGTDGSFGNEDSDKSVLSSIGRTIVPVLEPMGVEEENWPAAVGIFTGIFAKEAVVGTLNSLYGQMAATESADEEANESGFSFADTLGEALTVTGENLAGLGAALTDPLGLSVGDLSDETSAAEEQEVSTGTIDTIRTLFASESAAFAYLLLVLLYCPCVAAMATILREVGFVWTVFSGIWTTVLGYAAATVFYRLVHFAEAPAYASVAIGGSLAALLILLLVMKRLARRNTGPKVIPIVPKQ
>AP018786.1|BBF23055.1|1160726_1160963_-|iron-transporter-FeoA
MLLLKELPIGARGRIVGFHNEHREYRRRLLMLGATPGTTFEVVRRAPLGDPIEIRVRGSFISVRKDEAELMHIERLSD

Crispr_ID: AP018786_2

• CRISPR_ID: AP018786_2
• CRISPR_location: 2115913-2116921
• CRISPR_type: TypeI-E
• Repeat_type: I-C,I-E,II-B
• Spacer_info: 16 spacers
• Cas_protein_info: cas1,cas2,cas5,cas7,cas6e,cse2gr11,cas8e,cas3

Consensus repeat of AP018786_2

Consensus_repeat	Method
GGAAACACCCCCGCTTGCGCGGGGAATAC	CRISPRCasFinder
GGAAACACCCCCGCTTGCGCGGGGAATAC	CRT
GGAAACACCCCCGCTTGCGCGGGGAATAC	PILER-CR

spacers of AP018786_2

>2.1|2115942|32|AP018786|CRISPRCasFinder,CRT
ATATGGCGCGTGATTCGCGCTTGAAGCCTGCC
>2.2|2116003|32|AP018786|CRISPRCasFinder,CRT,PILER-CR
TGAAAGTTGAGGTCGACAACGAGAATCGCAAG
>2.3|2116064|32|AP018786|CRISPRCasFinder,CRT,PILER-CR
GCGGGCGCCTTTGCGGCGTTCAAACTCGTCAC
>2.4|2116125|32|AP018786|CRISPRCasFinder,CRT,PILER-CR
ATTCTCGCTACGCTTGACAAACGCACGTCTCA
>2.5|2116186|32|AP018786|CRISPRCasFinder,CRT,PILER-CR
TTGGTTTCCTCCTTTGGTGATCGAGTCGATGA
>2.6|2116247|34|AP018786|CRISPRCasFinder,CRT,PILER-CR
CACCCTGTGCGGCCGAAAAGGCCAACCGATGCCC
>2.7|2116310|32|AP018786|CRISPRCasFinder,CRT,PILER-CR
AATGCCGAGGACTGGGACGAGTGCGCTCATCA
>2.8|2116371|32|AP018786|CRISPRCasFinder,CRT,PILER-CR
GCGCGCCGCCAGCACAATGCCGCGATGGATGA
>2.9|2116432|32|AP018786|CRISPRCasFinder,CRT,PILER-CR
TGGCCGACCACAACGCGCCCGGTACGTACGAC
>2.10|2116493|32|AP018786|CRISPRCasFinder,CRT,PILER-CR
TTGGTATTGCTCCGAGCCCCGCAACCCGCGCC
>2.11|2116554|33|AP018786|CRISPRCasFinder,CRT,PILER-CR
CGACCGGCGTTGAACTTGATCGAGTCGGGCGTC
>2.12|2116616|32|AP018786|CRISPRCasFinder,CRT,PILER-CR
TTCCTTTGCCGCCTTGTTCTCAGACTGAAGCT
>2.13|2116677|33|AP018786|CRISPRCasFinder,CRT,PILER-CR
CACGCGAAAACGTGCGGAACGCCCGCAATCGTC
>2.14|2116739|32|AP018786|CRISPRCasFinder,CRT,PILER-CR
TTCATACCGAACTGCATGCCCGTCCAGTCCGC
>2.15|2116800|32|AP018786|CRISPRCasFinder,CRT,PILER-CR
ACGGGACCAAGACAAAGGCCGCCCGCCGAGCC
>2.16|2116861|32|AP018786|CRISPRCasFinder,CRT,PILER-CR
GGGATCGAAAAGGCCCGGAGCGTGTAGGTCCC

CRISPR arrays and Neighbor proteins around AP018786_2

CRISPR arrays

The CRISPR arrays of AP018786_2

>merge|AP018786|2|2115913-2116921|CRISPRCasFinder,CRT,PILER-CR
AGTCACACCCCCGCTTGCGCGGGGAATACATATGGCGCGTGATTCGCGCTTGAAGCCTGCCGGAAACACCCCCGCTTGCGCGGGGAATACTGAAAGTTGAGGTCGACAACGAGAATCGCAAGGGAAACACCCCCGCTTGCGCGGGGAATACGCGGGCGCCTTTGCGGCGTTCAAACTCGTCACGGAAACACCCCCGCTTGTGCGGGGAATACATTCTCGCTACGCTTGACAAACGCACGTCTCAGGAAACACCCCCGCTTGCGCGGGGAATACTTGGTTTCCTCCTTTGGTGATCGAGTCGATGAGGAAACACCCCCGCTTGCGCGGGGAATACCACCCTGTGCGGCCGAAAAGGCCAACCGATGCCCGGAAACACCCCCGCTTGCGCGGGGAATACAATGCCGAGGACTGGGACGAGTGCGCTCATCAGGAAACACCCCCGCTTGCGCGGGGAATACGCGCGCCGCCAGCACAATGCCGCGATGGATGAGGAAACACCCCCGCTTGCGCGGGGAATACTGGCCGACCACAACGCGCCCGGTACGTACGACGGAAACACCCCCGCTTGCGCGGGGAATACTTGGTATTGCTCCGAGCCCCGCAACCCGCGCCGGAAACACCCCCGCTTGCGCGGGGAATACCGACCGGCGTTGAACTTGATCGAGTCGGGCGTCAGAAACACCCCCGCTTGCGCGGGGAATACTTCCTTTGCCGCCTTGTTCTCAGACTGAAGCTGGAAACACCCCCGCTTGCGCGGGGAATACCACGCGAAAACGTGCGGAACGCCCGCAATCGTCGGAAACACCCCCGCTTGCGCGGGGAATACTTCATACCGAACTGCATGCCCGTCCAGTCCGCGGAAACACCCCCGCTTGCGCGGGGAATACACGGGACCAAGACAAAGGCCGCCCGCCGAGCCGGAAACACCCCCGCTTGCGCGGGGAATACGGGATCGAAAAGGCCCGGAGCGTGTAGGTCCCGGAAACACCCCCGCTTGCGCGGGGAATAC

>AP018786|2|2|2115913-2116921|CRISPRCasFinder
AGTCACACCCCCGCTTGCGCGGGGAATAC	ATATGGCGCGTGATTCGCGCTTGAAGCCTGCC
GGAAACACCCCCGCTTGCGCGGGGAATAC	TGAAAGTTGAGGTCGACAACGAGAATCGCAAG
GGAAACACCCCCGCTTGCGCGGGGAATAC	GCGGGCGCCTTTGCGGCGTTCAAACTCGTCAC
GGAAACACCCCCGCTTGTGCGGGGAATAC	ATTCTCGCTACGCTTGACAAACGCACGTCTCA
GGAAACACCCCCGCTTGCGCGGGGAATAC	TTGGTTTCCTCCTTTGGTGATCGAGTCGATGA
GGAAACACCCCCGCTTGCGCGGGGAATAC	CACCCTGTGCGGCCGAAAAGGCCAACCGATGCCC
GGAAACACCCCCGCTTGCGCGGGGAATAC	AATGCCGAGGACTGGGACGAGTGCGCTCATCA
GGAAACACCCCCGCTTGCGCGGGGAATAC	GCGCGCCGCCAGCACAATGCCGCGATGGATGA
GGAAACACCCCCGCTTGCGCGGGGAATAC	TGGCCGACCACAACGCGCCCGGTACGTACGAC
GGAAACACCCCCGCTTGCGCGGGGAATAC	TTGGTATTGCTCCGAGCCCCGCAACCCGCGCC
GGAAACACCCCCGCTTGCGCGGGGAATAC	CGACCGGCGTTGAACTTGATCGAGTCGGGCGTC
AGAAACACCCCCGCTTGCGCGGGGAATAC	TTCCTTTGCCGCCTTGTTCTCAGACTGAAGCT
GGAAACACCCCCGCTTGCGCGGGGAATAC	CACGCGAAAACGTGCGGAACGCCCGCAATCGTC
GGAAACACCCCCGCTTGCGCGGGGAATAC	TTCATACCGAACTGCATGCCCGTCCAGTCCGC
GGAAACACCCCCGCTTGCGCGGGGAATAC	ACGGGACCAAGACAAAGGCCGCCCGCCGAGCC
GGAAACACCCCCGCTTGCGCGGGGAATAC	GGGATCGAAAAGGCCCGGAGCGTGTAGGTCCC
GGAAACACCCCCGCTTGCGCGGGGAATAC

>AP018786|2|2|2115913-2116921|CRT
AGTCACACCCCCGCTTGCGCGGGGAATAC	ATATGGCGCGTGATTCGCGCTTGAAGCCTGCC
GGAAACACCCCCGCTTGCGCGGGGAATAC	TGAAAGTTGAGGTCGACAACGAGAATCGCAAG
GGAAACACCCCCGCTTGCGCGGGGAATAC	GCGGGCGCCTTTGCGGCGTTCAAACTCGTCAC
GGAAACACCCCCGCTTGTGCGGGGAATAC	ATTCTCGCTACGCTTGACAAACGCACGTCTCA
GGAAACACCCCCGCTTGCGCGGGGAATAC	TTGGTTTCCTCCTTTGGTGATCGAGTCGATGA
GGAAACACCCCCGCTTGCGCGGGGAATAC	CACCCTGTGCGGCCGAAAAGGCCAACCGATGCCC
GGAAACACCCCCGCTTGCGCGGGGAATAC	AATGCCGAGGACTGGGACGAGTGCGCTCATCA
GGAAACACCCCCGCTTGCGCGGGGAATAC	GCGCGCCGCCAGCACAATGCCGCGATGGATGA
GGAAACACCCCCGCTTGCGCGGGGAATAC	TGGCCGACCACAACGCGCCCGGTACGTACGAC
GGAAACACCCCCGCTTGCGCGGGGAATAC	TTGGTATTGCTCCGAGCCCCGCAACCCGCGCC
GGAAACACCCCCGCTTGCGCGGGGAATAC	CGACCGGCGTTGAACTTGATCGAGTCGGGCGTC
AGAAACACCCCCGCTTGCGCGGGGAATAC	TTCCTTTGCCGCCTTGTTCTCAGACTGAAGCT
GGAAACACCCCCGCTTGCGCGGGGAATAC	CACGCGAAAACGTGCGGAACGCCCGCAATCGTC
GGAAACACCCCCGCTTGCGCGGGGAATAC	TTCATACCGAACTGCATGCCCGTCCAGTCCGC
GGAAACACCCCCGCTTGCGCGGGGAATAC	ACGGGACCAAGACAAAGGCCGCCCGCCGAGCC
GGAAACACCCCCGCTTGCGCGGGGAATAC	GGGATCGAAAAGGCCCGGAGCGTGTAGGTCCC
GGAAACACCCCCGCTTGCGCGGGGAATAC

>AP018786|2|2|2115974-2116921|PILER-CR
GGAAACACCCCCGCTTGCGCGGGGAATAC	TGAAAGTTGAGGTCGACAACGAGAATCGCAAG
GGAAACACCCCCGCTTGCGCGGGGAATAC	GCGGGCGCCTTTGCGGCGTTCAAACTCGTCAC
GGAAACACCCCCGCTTGTGCGGGGAATAC	ATTCTCGCTACGCTTGACAAACGCACGTCTCA
GGAAACACCCCCGCTTGCGCGGGGAATAC	TTGGTTTCCTCCTTTGGTGATCGAGTCGATGA
GGAAACACCCCCGCTTGCGCGGGGAATAC	CACCCTGTGCGGCCGAAAAGGCCAACCGATGCCC
GGAAACACCCCCGCTTGCGCGGGGAATAC	AATGCCGAGGACTGGGACGAGTGCGCTCATCA
GGAAACACCCCCGCTTGCGCGGGGAATAC	GCGCGCCGCCAGCACAATGCCGCGATGGATGA
GGAAACACCCCCGCTTGCGCGGGGAATAC	TGGCCGACCACAACGCGCCCGGTACGTACGAC
GGAAACACCCCCGCTTGCGCGGGGAATAC	TTGGTATTGCTCCGAGCCCCGCAACCCGCGCC
GGAAACACCCCCGCTTGCGCGGGGAATAC	CGACCGGCGTTGAACTTGATCGAGTCGGGCGTC
AGAAACACCCCCGCTTGCGCGGGGAATAC	TTCCTTTGCCGCCTTGTTCTCAGACTGAAGCT
GGAAACACCCCCGCTTGCGCGGGGAATAC	CACGCGAAAACGTGCGGAACGCCCGCAATCGTC
GGAAACACCCCCGCTTGCGCGGGGAATAC	TTCATACCGAACTGCATGCCCGTCCAGTCCGC
GGAAACACCCCCGCTTGCGCGGGGAATAC	ACGGGACCAAGACAAAGGCCGCCCGCCGAGCC
GGAAACACCCCCGCTTGCGCGGGGAATAC	GGGATCGAAAAGGCCCGGAGCGTGTAGGTCCC
GGAAACACCCCCGCTTGCGCGGGGAATAC

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
AP018786.1|BBF23749.1|2119353_2119881_-|hypothetical-protein	cse2gr11	cd09731_cse2gr11_CAS-I-E	gnl|CDD|187862
AP018786.1|BBF23737.1|2107568_2107823_+|hypothetical-protein	unknown	unknown	unknown
AP018786.1|BBF23750.1|2119877_2121335_-|type-I-E-CRISPR-associated-protein-Cse1/CasA	cas8e	pfam09481_cas8e_CAS-I-E	gnl|CDD|187860
AP018786.1|BBF23744.1|2114629_2114908_-|hypothetical-protein	cas2	cd09648_cas2_CAS-I-E	gnl|CDD|378244
AP018786.1|BBF23742.1|2112328_2112718_+|hypothetical-protein	unknown	unknown	gnl|CDD|373118
AP018786.1|BBF23752.1|2124005_2125571_+|carboxylic-ester-hydrolase	unknown	unknown	gnl|CDD|225181
AP018786.1|BBF23755.1|2127486_2127912_+|hypothetical-protein	unknown	unknown	gnl|CDD|238089
AP018786.1|BBF23741.1|2110778_2112314_+|flavocytochrome-c	unknown	unknown	gnl|CDD|273816
AP018786.1|BBF23740.1|2110060_2110711_+|DNA-binding-response-regulator	unknown	unknown	gnl|CDD|223816
AP018786.1|BBF23745.1|2114901_2115774_-|CRISPR-associated-endonuclease-Cas1-2	cas1	cd09719_cas1_CAS-I-E	gnl|CDD|274693
AP018786.1|BBF23747.1|2117691_2118723_-|type-I-E-CRISPR-associated-protein-Cas7/Cse4/CasC	cas7	pfam09344_cas7_CAS-I-E	gnl|CDD|378153
AP018786.1|BBF23743.1|2112937_2114368_+|putative-S-methylmethionine-permease	unknown	unknown	gnl|CDD|236904
AP018786.1|BBF23748.1|2118733_2119354_-|type-I-E-CRISPR-associated-protein-Cas6/Cse3/CasE	cas6e	pfam08798_cas6e_CAS-I-E:CAS-IV	gnl|CDD|187858
AP018786.1|BBF23738.1|2107827_2109912_+|hypothetical-protein	unknown	unknown	gnl|CDD|223715
AP018786.1|BBF23739.1|2109880_2110105_-|hypothetical-protein	unknown	unknown	unknown
AP018786.1|BBF23753.1|2125695_2126445_+|SAM-dependent-methyltransferase	unknown	unknown	gnl|CDD|379312
AP018786.1|BBF23751.1|2121383_2123660_-|CRISPR-associated-helicase/endonuclease-Cas3	cas3	cd09639_cas3_CAS-I	gnl|CDD|182031
AP018786.1|BBF23736.1|2106536_2107499_+|permease	unknown	unknown	gnl|CDD|223769
AP018786.1|BBF23754.1|2126481_2126835_-|hypothetical-protein	unknown	unknown	unknown
AP018786.1|BBF23746.1|2116991_2117450_-|hypothetical-protein	cas5	cd09756_cas5_CAS-I-E	gnl|CDD|187886

HMMScan for Signature genes

Protein	Cas_name	Cas_description	E-value	Identity	Coverage
AP018786.1|BBF23748.1|2118733_2119354_-|type-I-E-CRISPR-associated-protein-Cas6/Cse3/CasE	cas6e	pfam08798_cas6e_CAS-I-E:CAS-IV	9.5e-34	112.1	0.0
AP018786.1|BBF23750.1|2119877_2121335_-|type-I-E-CRISPR-associated-protein-Cse1/CasA	cas8e	pfam09481_cas8e_CAS-I-E	6.3e-44	145.3	0.0
AP018786.1|BBF23749.1|2119353_2119881_-|hypothetical-protein	cse2gr11	cd09731_cse2gr11_CAS-I-E	4.7e-22	73.4	0.3
AP018786.1|BBF23751.1|2121383_2123660_-|CRISPR-associated-helicase/endonuclease-Cas3	cas3	cd09639_cas3_CAS-I	1.1e-56	187.2	0.0
AP018786.1|BBF23744.1|2114629_2114908_-|hypothetical-protein	cas2	cd09648_cas2_CAS-I-E	2.2e-17	57.7	0.0
AP018786.1|BBF23745.1|2114901_2115774_-|CRISPR-associated-endonuclease-Cas1-2	cas1	cd09719_cas1_CAS-I-E	1.3e-90	297.8	0.0
AP018786.1|BBF23746.1|2116991_2117450_-|hypothetical-protein	cas5	cd09756_cas5_CAS-I-E	1.3e-20	68.7	0.0
AP018786.1|BBF23747.1|2117691_2118723_-|type-I-E-CRISPR-associated-protein-Cas7/Cse4/CasC	cas7	pfam09344_cas7_CAS-I-E	2.7e-95	314.7	0.0

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
AP018786.1|BBF23749.1|2119353_2119881_-|hypothetical-protein	gnl|CDD|187862	cd09731, Cse2_I-E, CRISPR/Cas system-associated protein Cse2. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and associated Cas proteins comprise a system for heritable host defense by prokaryotic cells against phage and other foreign DNA; Small alpha-helical protein; also known as Cse2/CasB/YgcK family; specific gene for I-E subtype;.	6.69767e-18
AP018786.1|BBF23752.1|2124005_2125571_+|carboxylic-ester-hydrolase	gnl|CDD|225181	COG2272, PnbA, Carboxylesterase type B [Lipid metabolism].	4.339e-101
AP018786.1|BBF23750.1|2119877_2121335_-|type-I-E-CRISPR-associated-protein-Cse1/CasA	gnl|CDD|187860	cd09729, Cse1_I-E, CRISPR/Cas system-associated protein Cse1. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and associated Cas proteins comprise a system for heritable host defense by prokaryotic cells against phage and other foreign DNA; Large proteins, some contain Zn-finger domain; subunit of the Cascade complex; signature gene for I-E subtype; also known as Cse1/CasA/YgcL family.	1.53209e-28
AP018786.1|BBF23744.1|2114629_2114908_-|hypothetical-protein	gnl|CDD|378244	pfam09707, Cas_Cas2CT1978, CRISPR-associated protein (Cas_Cas2CT1978). This entry represents a minor branch of the Cas2 family of CRISPR-associated protein which are found in IPR003799. Cas proteins are found adjacent to a characteristic short, palindromic repeat cluster termed CRISPR, a probable mobile DNA element.	5.36412e-15
AP018786.1|BBF23742.1|2112328_2112718_+|hypothetical-protein	gnl|CDD|373118	pfam14537, Cytochrom_c3_2, Cytochrome c3.	3.93807e-11
AP018786.1|BBF23755.1|2127486_2127912_+|hypothetical-protein	gnl|CDD|238089	cd00158, RHOD, Rhodanese Homology Domain (RHOD); an alpha beta fold domain found duplicated in the rhodanese protein. The cysteine containing enzymatically active version of the domain is also found in the Cdc25 class of protein phosphatases and a variety of proteins such as sulfide dehydrogenases and certain stress proteins such as senesence specific protein 1 in plants, PspE and GlpE in bacteria and cyanide and arsenate resistance proteins. Inactive versions (no active site cysteine) are also seen in dual specificity phosphatases, ubiquitin hydrolases from yeast and in sulfuryltransferases, where they are believed to play a regulatory role in multidomain proteins.	1.33659e-28
AP018786.1|BBF23741.1|2110778_2112314_+|flavocytochrome-c	gnl|CDD|273816	TIGR01813, Fumarate_reductase_flavoprotein_subunit, flavocytochrome c. This model describes a family of redox proteins related to the succinate dehydrogenases and fumarate reductases of E. coli, mitochondria, and other well-characterized systems. A member of this family from Shewanella frigidimarina NCIMB400 is characterized as a water-soluble periplasmic protein with four heme groups, a non-covalently bound FAD, and essentially unidirectional fumarate reductase activity. At least seven distinct members of this family are found in Shewanella oneidensis, a species able to use a wide variety of pathways for respiraton. [Energy metabolism, Electron transport].	9.99721e-73
AP018786.1|BBF23740.1|2110060_2110711_+|DNA-binding-response-regulator	gnl|CDD|223816	COG0745, OmpR, Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain [Signal transduction mechanisms / Transcription].	3.18804e-53
AP018786.1|BBF23745.1|2114901_2115774_-|CRISPR-associated-endonuclease-Cas1-2	gnl|CDD|274693	TIGR03638, cas1_ECOLI, CRISPR-associated endonuclease Cas1, subtype I-E/ECOLI. The CRISPR-associated protein Cas1 is virtually universal to CRISPR systems. CRISPR, an acronym for Clustered Regularly Interspaced Short Palindromic Repeats, is prokaryotic immunity system for foreign DNA, mostly from phage. CRISPR systems belong to different subtypes, distinguished by both nature of the repeats, the makeup of the cohort of associated Cas proteins, and by molecular phylogeny within the more universal Cas proteins such as this one. This model is of type EXCEPTION and provides more specific information than the EQUIVALOG model TIGR00287. It describes the Cas1 protein particular to the ECOLI subtype of CRISPR/Cas system.	2.47923e-109
AP018786.1|BBF23747.1|2117691_2118723_-|type-I-E-CRISPR-associated-protein-Cas7/Cse4/CasC	gnl|CDD|378153	pfam09344, Cas_CT1975, CT1975-like protein. CRISPR is a term for Clustered, Regularly Interspaced Short Palidromic Repeats. A number of protein families appear only in association with these repeats and are designated Cas (CRISPR-Associated) proteins. This family is represented by CT1975 of Chlorobium tepidum.	3.73214e-110
AP018786.1|BBF23743.1|2112937_2114368_+|putative-S-methylmethionine-permease	gnl|CDD|236904	PRK11387, PRK11387, S-methylmethionine permease.	0
AP018786.1|BBF23748.1|2118733_2119354_-|type-I-E-CRISPR-associated-protein-Cas6/Cse3/CasE	gnl|CDD|187858	cd09727, Cas6_I-E, CRISPR/Cas system-associated RAMP superfamily protein Cas6e. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and associated Cas proteins comprise a system for heritable host defense by prokaryotic cells against phage and other foreign DNA; Cas6e is an endoribonuclease that generates crRNA; This family is specific for CRISPR/Cas system I-E subtype; Homologous to Cas6 (RAMP superfamily protein); Possesses double RRM/ferredoxin fold; also known as Cse3 family.	1.06322e-33
AP018786.1|BBF23738.1|2107827_2109912_+|hypothetical-protein	gnl|CDD|223715	COG0642, BaeS, Signal transduction histidine kinase [Signal transduction mechanisms].	7.75589e-38
AP018786.1|BBF23753.1|2125695_2126445_+|SAM-dependent-methyltransferase	gnl|CDD|379312	pfam13649, Methyltransf_25, Methyltransferase domain. This family appears to be a methyltransferase domain.	4.63812e-21
AP018786.1|BBF23751.1|2121383_2123660_-|CRISPR-associated-helicase/endonuclease-Cas3	gnl|CDD|182031	PRK09694, PRK09694, CRISPR-associated helicase/endonuclease Cas3.	8.94149e-82
AP018786.1|BBF23736.1|2106536_2107499_+|permease	gnl|CDD|223769	COG0697, RhaT, Permeases of the drug/metabolite transporter (DMT) superfamily [Carbohydrate transport and metabolism / Amino acid transport and metabolism / General function prediction only].	2.75815e-15
AP018786.1|BBF23746.1|2116991_2117450_-|hypothetical-protein	gnl|CDD|187886	cd09756, Cas5_I-E, CRISPR/Cas system-associated RAMP superfamily protein Cas5. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and associated Cas proteins comprise a system for heritable host defense by prokaryotic cells against phage and other foreign DNA; Cas5 is a RAMP superfamily protein; Subunit of the Cascade complex.	2.11317e-18

Protein sequences

>AP018786.1|BBF23745.1|2114901_2115774_-|CRISPR-associated-endonuclease-Cas1-2
MLGGFMARKIFLKLTRDLYPQVKDRYPYVYLEHGRLEVDDSSVKWIDSECHVVRIPIGLVAAILLGPGTTITHEAVKILSSAGCTVCWIGAEGLAFYASGVSPTSDSRNLLRQITMSCDPDSRLLVARRMFSQRFPGLDLGDKSIPEMMGMEGIRVRKMYSEMAQRYGVDWSGRAYIPGRPKDSDLVNSILTFSNGLLYGVATTVIVAMGYSPRVGFVHSGSPMPFVYDMADLYKKFLTIDLSFSMASRMDKSGFDRKSVIDEFCSRALEMNLLEVMANDIVEILREPKC
>AP018786.1|BBF23744.1|2114629_2114908_-|hypothetical-protein
MLVISAKDIPPSIRGKLKGFFIEVKPYVLVSGVSDALARKIVEEIVKACGNDYEMTILESLRRPPGYKVWYLGRPDGNKIIEITGMQLLLED
>AP018786.1|BBF23743.1|2112937_2114368_+|putative-S-methylmethionine-permease
MSADTTPHTSHAVDDSSFERTMKARHLIMLSLGGVIGTGLFFNTGFVIGEVGALGTVLAYLVGATLVYFVMLSLGELCVAMPHTGSFHLYTSKYISPRVGFTVAWLYWLTCTVCVGTSLLGAGLSMQYWFPDTPVWLWCLVFCVLVLGMNVFTSRLFAEGEFVFSLIKVVTIQVFIILGGLAVFGVLPLADGSPAPYFKNLTEAGPFPNGILPMVTTMVAVFFAFSGTELIGVAAGETENPQRVIPMAIRTTVVRLVVFFIGTIVILAALLPPEEATILKSPFVTIFERLGIPYAASIFNFVILTAVVSAANANLFASGRMLWSLSKDGMLPKALAKQSKNGLPVTALVVSMLGGWATLVTSVVAADTVFVFLTALCGFSVVVVWMSISLAHINFRKRWLEEGRSLDELPYRAPWFPFVPVAAIVLCCIACVGLAFDPEQRLALIVGLPFFIGCLLVYPKLAARRPQHGEPQEVAP
>AP018786.1|BBF23742.1|2112328_2112718_+|hypothetical-protein
MKKHLLTAAFAALLLTPTFQASAVDLKHKGFECSTCHAKGFTAPDRSTCLTCHKSDDIVKATERFNFTTSFTNPVTGETKTSIAPVNPHDNFHFGRDDQCVNCHKEHKPSTTTCQTCHDIEPWGLKAPR
>AP018786.1|BBF23741.1|2110778_2112314_+|flavocytochrome-c
MQTSRRSFLGRAAAGLGLAGSGSLLAPTARANESTAPAFDAVYDIIVVGSGISGTMAALVAARSGAKTLLIEKLNRLGGTSRYSALDFSCVGSDRQKAEGIEDTPEAMVDDMAKVAGGLADRERALNVARNTARAQKVMAEHGVKWKNLLKLGGHSAKRCLIAEGGGAGILRSLWASFERYPNLTVMPSTKLDELILDETGVTGVVVRTHYLFDASLPNDDRDNRSGAVKRFGARQGVIMATGGYARDKEFMKDEVPYLEHTSNSCSEGATAGALKSMIRAGARPVQLGLYRFSFALPTEDFIWGVAIDAKTGKRYCAESLGRNAIALASMRVRQSSGKMPFIVYDDAALELFHNLGRAAKSLAGLNGRNGTMYKFDTLEALARHFETDPKALRATIDAYNADIAAGRDSAFGKDLERSGRKVRPIGKTGPFYGIPMTARWDYCPGGILTDTEARALKLSDGTPLPGLWVVGEAAGGIHGAERLTACSMPDCSVTGMLAAESAVNSAKKSL
>AP018786.1|BBF23740.1|2110060_2110711_+|DNA-binding-response-regulator
MSEACDGKTALELFHTLSVKPDLVLLDLMMPEMDGHEALARLRETSDVPVIILTARDLFDDKRRTFTTGADDYLVKPFSLPELELRIRALLRRSGHGADRSEESRISTGDFLLDAERCRIEWRGRSAALTGRELRLLTPLVSRPGNVVRYEELLRVGWPDDPYADTSHLRVAIARLRKKIAGLGLSPRVLSSYSSVGYLLGDLSNYDDDYGDESDD
>AP018786.1|BBF23739.1|2109880_2110105_-|hypothetical-protein
MEKLKGGLAVAGFAHGKSCGPEMLGDKLANARFVVGDEDEGLGHKCLANLRRCKKAVRQPARSVVKARVAAVEA
>AP018786.1|BBF23738.1|2107827_2109912_+|hypothetical-protein
MRFRSYIALALTATAIVPFFAWSVLQRGDVDRRILESDAEQSRAAHEAARLVEERLETLAQLARYAARSVGEAARAEPTPVSPEKVELLLRDVVKSFPTLNNLHVDRLAPESARVAAFYPPHASDGRALLGVDHSTRWHVAAKRRGLEGVAFSPVLTSSQNREAPPILTFAADVPGSPELLLSGAVELEALLHEIEGSLSSRGLQLTVLTPERQIVWPASTAPHVRTWPEVPDDRSLIGLGSARRFVTVAHLPDSVSGWAIVVSKPESLRSAERRRIERRTALLASAVLLLTLVAGWAAGRPLRRAFRQLEEDLDARGFGTDRTTIRRGPDELRHVQRVYREVRRALDARNAQLSATAEQRARELEAEELLFRSVFDGIELPFLLLDADWKPHLRNRAATSLNDRVAERLVDEARAHWTTPDAADTTTVDVRNDDGRLETFALHVFPFSARRSTLAEGYALLVESVTDREALKRMKDDLLGIVAHELKTPVTACRLQVERLESTLGPHEGLRALDRDLDHLRHIVHDWLSVARIDGGTFTVYPSVVQLDPLLRKARRLVRARYDFDFSVSVDEEAECIFADASALTDVFVNLFTNACRYAKPDKKPVVAVTARREGNDIVIDVCDRGIGIAPEDLERIFDRFYQVSQGTRRRTGGTGLGLVICRAVCTAHGGTLEASCAGGVTRFTLRLPQPSP
>AP018786.1|BBF23737.1|2107568_2107823_+|hypothetical-protein
MSDIVLTLSVPAETLERARAVCERDGTDLETELLRRIELIASRDREARSILVTPDAADRLAELMQDPASGGIEPSAAQMLRHLK
>AP018786.1|BBF23736.1|2106536_2107499_+|permease
MLFELRQSLLLLLTATIWGLSFVAQSVGMDHVGPFTFTASRMALGTLVLLPWVFFARRRLARNRPDTYAERTTPAYRKRLLLGGFLCSLCLFAGESLQQFGLVHDTEVGKAGFITALYIVIVPFLGFFLGKRVSKLVLVAVVLSLVGLWYLCVPPEGFSIELGDFFIFLCAFVFSLHILVISHFVTRVDGVELAVTQFAFGSVIAALMMLIFEDPTIEGLRGALIAILWAGIMSNGLAYTLQIVGQRGMNDTVASLIMSLESVVSVLAGWAILGEVLSERELLGCVLMAGAVVLAQLPQPKSAGKPQTESRLGSDATEAA
>AP018786.1|BBF23746.1|2116991_2117450_-|hypothetical-protein
MVGNGYDDRDPWSYLRIPKKSDGKKANNGGAKLTRRYYLQDAAFACILTVPERWSIAMVNGLKNPKWPVYLGRKGCPPSEPIFSGCFATPDEARLGLIELLKESSWIPFEKITEDWSGGHIGGMVLYDVPVTFGMHKKYASRKVIRTPIAEV
>AP018786.1|BBF23747.1|2117691_2118723_-|type-I-E-CRISPR-associated-protein-Cas7/Cse4/CasC
MKPIIEFHIIQSFPVSCLNRDDVGSPKTVTIGGAPRIRVSSQCWKRAVRTKLHELGVTLGIRTKKVAEVLAAKLQEKTGTEQKKVASAMAKILSDDTLLYLTDSEYNKIVDCLLECNLDVSSSEKKIKALLKKLSATPYDGLDIALFGRMVAKAAEMNVEAACSFNHAFSTHKAAPELDFFSGLDDLREEDEAGGGHLGVNEFSSATFYRYISLDVQQLIDTLGLEKREDIDYAISSFVKAIYLAVPSARQKTMAASSVWDFCRCYIRKGQPLQCAFDSPVKPGNGGGYIEPSIKTMLSELDSKESRCGSLFGKKSCIDFGEGTECQNIDVLIKNILDAVHHD
>AP018786.1|BBF23748.1|2118733_2119354_-|type-I-E-CRISPR-associated-protein-Cas6/Cse3/CasE
MQLYATQMILSPMAQIRLKVTDTYSLHRLVMDLFPHRYDGQGSELPSGIQWVDCGEHIFGREIRILSKVSPCVLNFGNSGVEDCKIITNPILESFFDKETYRFSLVANPFVCKGSGQRIPIRDELEIGKWLDMKAEGSGFKAVEKNVDRIRPLIFNKGRNRITLHTARISGVLKVQEKEKFIETVGRGIGRGRAWGLGLLQLSLKI
>AP018786.1|BBF23749.1|2119353_2119881_-|hypothetical-protein
MKSESTSSNRAAAFVQNMIELCKRSNGARSALRRAASQEQEACIYMYLSPWIDLTDNRKRKIYTLVASMLASEKDVGSGSLSLGKVLQLAVKKRIKKDGSNKEPPSDPRFRRLLATDGVDDALLVLRPIIRLIQARCPGQLDYVSLLRDLEWYDERVRIRWARDFFRTVEEQEDI
>AP018786.1|BBF23750.1|2119877_2121335_-|type-I-E-CRISPR-associated-protein-Cse1/CasA
MFSLLTKPWIPVAGRDPVSLNELFENDEAYELCGPVADQLACFLFLRALTQRACEDALEYREDWQDLTSKVFRRKVLDYLHKHEALFDLEGEKPFLQYVALRSMKAEKSWNLSELRVGVSTGNTTILTERESSSPLTVSELVCSILRLVAFPWGGKYHDKSITLQQGLIKNKATGRALTIGMYGYLHNFAWADGLVETLRYNLLTKEDIDKVKYIKGMGVPAWERLPDDEGDFRFTLYEFLFPVSRFFLIQGDRVIVTDGVRPDLSPGMVPPSVSVREFMTSKKNNKSKIETLDAKCFSLPWRHIGEILPFYSKETKGWHCVQTEAVRDRKGRKGVWSLGIQVSYKSGDQYVTANDGFVSQVFKFPDEESSAEVAYARYHVEMKRLEKLVGLLSKATNSYWSDLRIGKNLPERIKQAEQNFWFECDKLKDELEILCDTGDVKAYRAKTRELVGKSFDRMCPKIPGIKRLEAWAKNRPFLKTKDAK
>AP018786.1|BBF23751.1|2121383_2123660_-|CRISPR-associated-helicase/endonuclease-Cas3
MAAAHDVGKMNPLFLKKLLDSLPAESTEVKHWSEVLRGFPTDLQEVPHPCVSRTAVADESVRAIGEVVERHHGYEPAEAEDPAEYVAAGGRLWQEYRAEALAWYKHELGIDSFPRPPRSKARRAMLDLWAGTVTLADWIASQVSEPLEEADVAEKARALVSRAGFEKIRVEGELDFRALFGFEPRAFQKEAGELYAGPGLYILEAPMGYGKTEAALWLAYRALADGRARGIYFAMPTQLTSTTLWSRFARAVRTMTSSNIPVNLVHQAARLLEAAMGKEAAAGGEWASTPRRALLGNFGVGTIDQCLLAGLPVRFHTVRWAGLVPKVVVFDEVHSYDAYTTTILQSVVHTLRLLGCTVIILSATLTRRVRYELLGEEAKGVVEENSDGPIRLTAMTESSYTVRVLPSERKLDVHLRVTDDQAVVEKDVIARVRRGERVLWIENTVGDAQAVFRRLSEVIPSGLLHGRFRPVERLSLEESWTAAFAPKAEERSQKGLLLVGTQVLEMSLDLDADYLVTRIAPMDHVLQRMGRLWRHERTVRPSGCAIPGMTVLVPGPDEIARGEFGTSGVIYAPYVLWKTYRALEARDRICFPDDISELLETAYGNDDELPEPIRRLLEDVDAKRLRMSGSALVKMSISGGSDRVPCTRWIEGPEDELLILDTEFPAENASSAELTLWCEERLVRTPFVLVRDELPELPAKLRKFLTESPRFGRNRTAVGLAAPGGEIRGVRTVRGRLIYTKREGLFTELSEEEVCVRQ
>AP018786.1|BBF23752.1|2124005_2125571_+|carboxylic-ester-hydrolase
MITRRNLLCTSGALLATTLAGCAATPASVESTGAADDIIALPSGKIRGSVRNGIRVFRNVPFGANPYTPERRFRHSVPPEPWTGVRDCTKPGAIPLQPDRNGPGMIGGGDALVLNLWAPAGGKDLPVMVWIPGGGSTNCDNNDLRFDGTRFAEDGVVLVTLNYRVNVDGFLKLEDGDANCGLSDMILGLKWVHDNIALFGGDPNRVTVFGQSAGGTHTTSLIASPKTKGLFSQAIIQSPSAVAQWRNEADARKAAKRLADFLGVPPTRAGFASLTNDELVAFKKLVGQLSKDPDWSLFSQGNTALFKPYVDHDILQDRPVDAIRAGAAAGIRVISGCTKEEWRNYVVPNGAIDRIGYDAVKMLTASLGLPSSIAEDYRAAGHGRTTGEVFTRMQGDLIFRMPCNRLLESLAISGTPVWAYSFEWESPVTGKTGQKRGAAHSSDVSFVFDTIAAPTAVAINGADAPQALADAMHGAWVRFAKTGNPGWEPFDLECRATRRFADEVSTVLDPWAFERKTLIVP
>AP018786.1|BBF23753.1|2125695_2126445_+|SAM-dependent-methyltransferase
MSALYDDPAFFSAYAAMPRSRLGLAGAPEWHALHALLPEARGLDVLDLGCGYGWHAAHFAREGARRVLALDASKRMLERARTENADPRIEYEPCDLNDPEAVKALPGRGPFDLVLASLVIHYLDDYPGLVRVVHNTLRPGGTFAFNVEHPVFTAQGSQDWIYGPGGEIEYFPVDRYAFEGPRETNFLGNRVRKVHRTLGTYLQTLLDAGFVLRNVVEPKPLPEAVAADPRLADELRRPMMLLVRADKPA
>AP018786.1|BBF23754.1|2126481_2126835_-|hypothetical-protein
MISNRRRLLTAAAAAVFSTQTVRAAETVIGTILEEVSRDQLQRFADEYRNDWKRDRDGTYEYRGRRYNESEWEVYWRKRYREEKRKDRRDREDEVRRRDEEVRRRDALQRLKDAAQR
>AP018786.1|BBF23755.1|2127486_2127912_+|hypothetical-protein
MNLRIAAAAFSAVAMLCSSVYAQEPVNPQPATPLTYENPAYQKITPDVAKKMMEEGGVVVIDVREPNEYAEGHVKGAVNVPLSTLKPGVKLEAAPDLNQKVLVQCKSGVRAEKASKILVESGYKHVYNFYGTSQWPFGLVK

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity
AP018786_2	2.5|2116186|32|AP018786|CRISPRCasFinder,CRT,PILER-CR	2116186-2116217	32	NZ_KY362367	Pseudomonas coronafaciens pv. garcae strain 2708 plasmid pPg2708, complete sequence	35952-35983	7	0.781
AP018786_2	2.5|2116186|32|AP018786|CRISPRCasFinder,CRT,PILER-CR	2116186-2116217	32	CP050261	Pseudomonas coronafaciens strain X-1 plasmid unnamed1	41043-41074	7	0.781
AP018786_2	2.5|2116186|32|AP018786|CRISPRCasFinder,CRT,PILER-CR	2116186-2116217	32	CP046442	Pseudomonas coronafaciens pv. coronafaciens strain B19001 plasmid unnamed1, complete sequence	55067-55098	7	0.781
AP018786_2	2.8|2116371|32|AP018786|CRISPRCasFinder,CRT,PILER-CR	2116371-2116402	32	LR134127	Klebsiella aerogenes strain NCTC10006 genome assembly, plasmid: 7	149657-149688	7	0.781
AP018786_2	2.8|2116371|32|AP018786|CRISPRCasFinder,CRT,PILER-CR	2116371-2116402	32	NZ_CP016500	Agrobacterium sp. RAC06 plasmid pBSY240_1, complete sequence	317960-317991	7	0.781
AP018786_2	2.8|2116371|32|AP018786|CRISPRCasFinder,CRT,PILER-CR	2116371-2116402	32	NZ_CP032703	Pantoea dispersa strain DSM 32899 plasmid unnamed1, complete sequence	502878-502909	7	0.781
AP018786_2	2.8|2116371|32|AP018786|CRISPRCasFinder,CRT,PILER-CR	2116371-2116402	32	NZ_CP045856	Agrobacterium sp. MA01 plasmid punanmed2, complete sequence	50112-50143	7	0.781
AP018786_2	2.8|2116371|32|AP018786|CRISPRCasFinder,CRT,PILER-CR	2116371-2116402	32	NC_018580	Gordonia sp. KTR9 plasmid pGKT2, complete sequence	21996-22027	8	0.75
AP018786_2	2.8|2116371|32|AP018786|CRISPRCasFinder,CRT,PILER-CR	2116371-2116402	32	NZ_CP016365	Phaeobacter porticola strain P97 plasmid pP97_a, complete sequence	21637-21668	8	0.75
AP018786_2	2.16|2116861|32|AP018786|CRISPRCasFinder,CRT,PILER-CR	2116861-2116892	32	NZ_CP045095	Streptomyces sp. GY16 plasmid unnamed1, complete sequence	36020-36051	8	0.75
AP018786_1	1.1|1149236|32|AP018786|PILER-CR,CRISPRCasFinder,CRT	1149236-1149267	32	KF114879	Leptospira phage LbrZ_5399, complete genome	68041-68072	9	0.719
AP018786_1	1.1|1149236|32|AP018786|PILER-CR,CRISPRCasFinder,CRT	1149236-1149267	32	KF114880	Leptospira phage LinZ_10, complete genome	50284-50315	9	0.719
AP018786_1	1.1|1149236|32|AP018786|PILER-CR,CRISPRCasFinder,CRT	1149236-1149267	32	KX656785	UNVERIFIED: Leptospira phage Lin_34, complete genome	57385-57416	9	0.719
AP018786_2	2.8|2116371|32|AP018786|CRISPRCasFinder,CRT,PILER-CR	2116371-2116402	32	NZ_CP049029	Fluviibacterium aquatile strain SC52 plasmid pSC52_1, complete sequence	68783-68814	9	0.719
AP018786_2	2.8|2116371|32|AP018786|CRISPRCasFinder,CRT,PILER-CR	2116371-2116402	32	NZ_CP022418	Sulfitobacter pseudonitzschiae strain SMR1 plasmid pSMR1-3, complete sequence	6716-6747	9	0.719
AP018786_2	2.10|2116493|32|AP018786|CRISPRCasFinder,CRT,PILER-CR	2116493-2116524	32	NZ_CP022367	Azospirillum sp. TSH58 plasmid TSH58_p02, complete sequence	356435-356466	9	0.719
AP018786_2	2.11|2116554|33|AP018786|CRISPRCasFinder,CRT,PILER-CR	2116554-2116586	33	NZ_AP022593	Mycolicibacterium arabiense strain JCM 18538 plasmid pJCM18538, complete sequence	2137553-2137585	9	0.727
AP018786_2	2.12|2116616|32|AP018786|CRISPRCasFinder,CRT,PILER-CR	2116616-2116647	32	NC_014502	Gloeothece verrucosa PCC 7822 plasmid Cy782203, complete sequence	70892-70923	9	0.719
AP018786_2	2.14|2116739|32|AP018786|CRISPRCasFinder,CRT,PILER-CR	2116739-2116770	32	NZ_CP030365	Salinibacter ruber strain M1 plasmid pSR116, complete sequence	65341-65372	9	0.719
AP018786_2	2.8|2116371|32|AP018786|CRISPRCasFinder,CRT,PILER-CR	2116371-2116402	32	NC_016587	Azospirillum lipoferum 4B plasmid AZO_p4, complete sequence	257281-257312	10	0.688
AP018786_2	2.8|2116371|32|AP018786|CRISPRCasFinder,CRT,PILER-CR	2116371-2116402	32	NZ_CP029833	Azospirillum ramasamyi strain M2T2B2 plasmid unnamed3, complete sequence	138838-138869	10	0.688
AP018786_2	2.8|2116371|32|AP018786|CRISPRCasFinder,CRT,PILER-CR	2116371-2116402	32	NC_013858	Azospirillum sp. B510 plasmid pAB510d, complete sequence	179822-179853	10	0.688
AP018786_2	2.8|2116371|32|AP018786|CRISPRCasFinder,CRT,PILER-CR	2116371-2116402	32	NZ_CP012399	Chelatococcus sp. CO-6 plasmid pCO-6, complete sequence	15387-15418	10	0.688
AP018786_2	2.8|2116371|32|AP018786|CRISPRCasFinder,CRT,PILER-CR	2116371-2116402	32	NZ_CP024310	Sinorhizobium fredii strain NXT3 plasmid pSfreNXT3c, complete sequence	243299-243330	10	0.688
AP018786_2	2.9|2116432|32|AP018786|CRISPRCasFinder,CRT,PILER-CR	2116432-2116463	32	NC_018022	Mycolicibacterium chubuense NBB4 plasmid pMYCCH.01, complete sequence	589968-589999	10	0.688
AP018786_2	2.12|2116616|32|AP018786|CRISPRCasFinder,CRT,PILER-CR	2116616-2116647	32	MN234234	Arthrobacter phage Lymara, complete genome	58927-58958	10	0.688

1. spacer 2.5|2116186|32|AP018786|CRISPRCasFinder,CRT,PILER-CR matches to NZ_KY362367 (Pseudomonas coronafaciens pv. garcae strain 2708 plasmid pPg2708, complete sequence) position: , mismatch: 7, identity: 0.781

ttggtttcctcctttggtgatcgagtcgatga	CRISPR spacer
ttcagtgcctcctttgatgatcgagtccatgg	Protospacer
** . * *********.********** ***.

2. spacer 2.5|2116186|32|AP018786|CRISPRCasFinder,CRT,PILER-CR matches to CP050261 (Pseudomonas coronafaciens strain X-1 plasmid unnamed1) position: , mismatch: 7, identity: 0.781

ttggtttcctcctttggtgatcgagtcgatga	CRISPR spacer
ttcagtgcctcctttgatgatcgagtccatgg	Protospacer
** . * *********.********** ***.

3. spacer 2.5|2116186|32|AP018786|CRISPRCasFinder,CRT,PILER-CR matches to CP046442 (Pseudomonas coronafaciens pv. coronafaciens strain B19001 plasmid unnamed1, complete sequence) position: , mismatch: 7, identity: 0.781

ttggtttcctcctttggtgatcgagtcgatga	CRISPR spacer
ttcagtgcctcctttgatgatcgagtccatgg	Protospacer
** . * *********.********** ***.

4. spacer 2.8|2116371|32|AP018786|CRISPRCasFinder,CRT,PILER-CR matches to LR134127 (Klebsiella aerogenes strain NCTC10006 genome assembly, plasmid: 7) position: , mismatch: 7, identity: 0.781

gcgcgccgccagcacaatgccgc-----gatggatga	CRISPR spacer
gcgcgccaccagcacaaagccgcccccagatg-----	Protospacer
*******.********* *****     ****     

5. spacer 2.8|2116371|32|AP018786|CRISPRCasFinder,CRT,PILER-CR matches to NZ_CP016500 (Agrobacterium sp. RAC06 plasmid pBSY240_1, complete sequence) position: , mismatch: 7, identity: 0.781

gcgcgccgc-cagcacaatgccgcgatggatga	CRISPR spacer
-cagaccacgcagcagaatgccgcgatggttga	Protospacer
 *. .**.* ***** ************* ***

6. spacer 2.8|2116371|32|AP018786|CRISPRCasFinder,CRT,PILER-CR matches to NZ_CP032703 (Pantoea dispersa strain DSM 32899 plasmid unnamed1, complete sequence) position: , mismatch: 7, identity: 0.781

-gcgcgccgccagcacaatgccgcgatggatga	CRISPR spacer
agcaatctg-cagcaaaatgccgcgatggttga	Protospacer
 **.  *.* ***** ************* ***

7. spacer 2.8|2116371|32|AP018786|CRISPRCasFinder,CRT,PILER-CR matches to NZ_CP045856 (Agrobacterium sp. MA01 plasmid punanmed2, complete sequence) position: , mismatch: 7, identity: 0.781

gcgcgccgc-cagcacaatgccgcgatggatga	CRISPR spacer
-cagaccacgcagcagaatgccgcgatggttga	Protospacer
 *. .**.* ***** ************* ***

8. spacer 2.8|2116371|32|AP018786|CRISPRCasFinder,CRT,PILER-CR matches to NC_018580 (Gordonia sp. KTR9 plasmid pGKT2, complete sequence) position: , mismatch: 8, identity: 0.75

gcgcgccgccagcacaatgccgcgatggatga	CRISPR spacer
gcgcgccgccagcgcaacgccgccgacgacaa	Protospacer
*************.***.***** .  **..*

9. spacer 2.8|2116371|32|AP018786|CRISPRCasFinder,CRT,PILER-CR matches to NZ_CP016365 (Phaeobacter porticola strain P97 plasmid pP97_a, complete sequence) position: , mismatch: 8, identity: 0.75

-gcgcgccgccagcacaatgccgcgatggatga	CRISPR spacer
caggtgac-tcagcaaaatgccgcgatggttga	Protospacer
 . *.* * .***** ************* ***

10. spacer 2.16|2116861|32|AP018786|CRISPRCasFinder,CRT,PILER-CR matches to NZ_CP045095 (Streptomyces sp. GY16 plasmid unnamed1, complete sequence) position: , mismatch: 8, identity: 0.75

gggatcgaaaaggcccggagcgtgtaggtccc	CRISPR spacer
gggatcgacatggcccggagcgtagcggggac	Protospacer
******** * ************.  **   *

11. spacer 1.1|1149236|32|AP018786|PILER-CR,CRISPRCasFinder,CRT matches to KF114879 (Leptospira phage LbrZ_5399, complete genome) position: , mismatch: 9, identity: 0.719

acgtagatccgcttgacttcggtaaaaagctc	CRISPR spacer
ttttagatccgcttgatttcggtaaagcgaaa	Protospacer
 . *************.*********. *   

12. spacer 1.1|1149236|32|AP018786|PILER-CR,CRISPRCasFinder,CRT matches to KF114880 (Leptospira phage LinZ_10, complete genome) position: , mismatch: 9, identity: 0.719

acgtagatccgcttgacttcggtaaaaagctc	CRISPR spacer
ttttagatccgcttgatttcggtaaagcgaaa	Protospacer
 . *************.*********. *   

13. spacer 1.1|1149236|32|AP018786|PILER-CR,CRISPRCasFinder,CRT matches to KX656785 (UNVERIFIED: Leptospira phage Lin_34, complete genome) position: , mismatch: 9, identity: 0.719

acgtagatccgcttgacttcggtaaaaagctc	CRISPR spacer
ttttagatccgcttgatttcggtaaagcgaaa	Protospacer
 . *************.*********. *   

14. spacer 2.8|2116371|32|AP018786|CRISPRCasFinder,CRT,PILER-CR matches to NZ_CP049029 (Fluviibacterium aquatile strain SC52 plasmid pSC52_1, complete sequence) position: , mismatch: 9, identity: 0.719

gcgcg-ccgccagcacaatgccgcgatggatga	CRISPR spacer
-cacatccgccagcacagtgtcgcgatggtgtt	Protospacer
 *.*. ***********.**.********    

15. spacer 2.8|2116371|32|AP018786|CRISPRCasFinder,CRT,PILER-CR matches to NZ_CP022418 (Sulfitobacter pseudonitzschiae strain SMR1 plasmid pSMR1-3, complete sequence) position: , mismatch: 9, identity: 0.719

gcgcgccgccagcacaatgccgcgatggatga	CRISPR spacer
ccgcgccgccagcacaaagtcgcggccggcca	Protospacer
 **************** *.****.. *.. *

16. spacer 2.10|2116493|32|AP018786|CRISPRCasFinder,CRT,PILER-CR matches to NZ_CP022367 (Azospirillum sp. TSH58 plasmid TSH58_p02, complete sequence) position: , mismatch: 9, identity: 0.719

ttggtattgctccgagccccgcaacccgcgcc	CRISPR spacer
accgtcgcgctccaagccccggaacccgcgca	Protospacer
 . **  .*****.******* ********* 

17. spacer 2.11|2116554|33|AP018786|CRISPRCasFinder,CRT,PILER-CR matches to NZ_AP022593 (Mycolicibacterium arabiense strain JCM 18538 plasmid pJCM18538, complete sequence) position: , mismatch: 9, identity: 0.727

cgaccggcgttgaacttgatcgagtcgggcgtc	CRISPR spacer
ccgtcggcgtcgaacttgaccgagtcgggtacg	Protospacer
* ..******.********.*********... 

18. spacer 2.12|2116616|32|AP018786|CRISPRCasFinder,CRT,PILER-CR matches to NC_014502 (Gloeothece verrucosa PCC 7822 plasmid Cy782203, complete sequence) position: , mismatch: 9, identity: 0.719

ttcctttgccgccttgttctcagactgaagct	CRISPR spacer
ttcttttgccgccttgttcccagtcgatatag	Protospacer
***.***************.*** * . *   

19. spacer 2.14|2116739|32|AP018786|CRISPRCasFinder,CRT,PILER-CR matches to NZ_CP030365 (Salinibacter ruber strain M1 plasmid pSR116, complete sequence) position: , mismatch: 9, identity: 0.719

ttcataccgaactgcatgcccgtccagtccgc	CRISPR spacer
acctcagtaaactgcatgcgcggccagtccgc	Protospacer
 .* .* ..********** ** *********

20. spacer 2.8|2116371|32|AP018786|CRISPRCasFinder,CRT,PILER-CR matches to NC_016587 (Azospirillum lipoferum 4B plasmid AZO_p4, complete sequence) position: , mismatch: 10, identity: 0.688

gcgcgccgccagcacaatgccgcgatggatga	CRISPR spacer
gcgcgccgccagcacgatgtcgcaggccagcg	Protospacer
***************.***.***..   *  .

21. spacer 2.8|2116371|32|AP018786|CRISPRCasFinder,CRT,PILER-CR matches to NZ_CP029833 (Azospirillum ramasamyi strain M2T2B2 plasmid unnamed3, complete sequence) position: , mismatch: 10, identity: 0.688

gcgcgccgccagcacaatgccgcgatggatga	CRISPR spacer
gcgcgccgccagcacgatgtcgcaggccagcg	Protospacer
***************.***.***..   *  .

22. spacer 2.8|2116371|32|AP018786|CRISPRCasFinder,CRT,PILER-CR matches to NC_013858 (Azospirillum sp. B510 plasmid pAB510d, complete sequence) position: , mismatch: 10, identity: 0.688

gcgcgccgccagcacaatgccgcgatggatga	CRISPR spacer
gcgcgccgccagcacgatgtcgcaggccagcg	Protospacer
***************.***.***..   *  .

23. spacer 2.8|2116371|32|AP018786|CRISPRCasFinder,CRT,PILER-CR matches to NZ_CP012399 (Chelatococcus sp. CO-6 plasmid pCO-6, complete sequence) position: , mismatch: 10, identity: 0.688

gcgcgccgccagcacaatgccgcgatggatga	CRISPR spacer
ctccgccgccggcacaatgcctcgatcctcgc	Protospacer
 . *******.********** ****   .* 

24. spacer 2.8|2116371|32|AP018786|CRISPRCasFinder,CRT,PILER-CR matches to NZ_CP024310 (Sinorhizobium fredii strain NXT3 plasmid pSfreNXT3c, complete sequence) position: , mismatch: 10, identity: 0.688

gcgcgccgccagcacaatgccgcgatggatga	CRISPR spacer
tgttgaagccagcgcaaggccgcgatggattg	Protospacer
   .*  ******.*** ************ .

25. spacer 2.9|2116432|32|AP018786|CRISPRCasFinder,CRT,PILER-CR matches to NC_018022 (Mycolicibacterium chubuense NBB4 plasmid pMYCCH.01, complete sequence) position: , mismatch: 10, identity: 0.688

tggccgaccacaacgcgcccggtacgtacgac	CRISPR spacer
tggccgagcacaacgcgcgcggttggcgtctt	Protospacer
******* ********** ****  *...  .

26. spacer 2.12|2116616|32|AP018786|CRISPRCasFinder,CRT,PILER-CR matches to MN234234 (Arthrobacter phage Lymara, complete genome) position: , mismatch: 10, identity: 0.688

ttcctttgccgccttgttctcagactgaagct	CRISPR spacer
gaacagcaccaccttgttctcagactcaagca	Protospacer
   *  ..**.*************** **** 

Prophage detection

Region	Region Position	Protein_number	Hit_taxonomy	Key_proteins	Att_site
DBSCAN-SWA_1	733379 : 742478	7	Dickeya_phage(16.67%)	NA	NA

Sequences of DBSCAN-SWA_1

Nucleotide sequences of DBSCAN-SWA_1

>AP018786|733379:742478|DBSCAN-SWA
CATGTCTCAAACGATTCTGCAAAGCGTTCCGGTCGGTCAGAAGGTCGGCATCGCGTTTTCCGGCGGCCTTGATACGAGCGCTGCTCTTCGTTGGATGAAAAATAAGGGTGCTCTCCCCTACGCCTACACCGCCAACCTCGGCCAGCCCGACGAAAAGGACTACGAAGCCATTCCGCGTCGCGCCATGGAGTACGGCGCCGAAAACGCCCGCCTGATCGACTGCCGTCCGCAGCTCGTCAACGAAGGCATCGCCGCCATCCAGTCGGGCGCCTTCCACATCTCGACGGGCGGTCAGCTCTACTTCAACACGACGCCGATCGGCCGCGCCGTGACGGGCACGATGCTCGTTGCCGCCATGCGCGAAGACGGCGTGCACATCTGGGGCGACGGCTCGACCTACAAGGGCAACGACATCGAACGCTTCTACCGTTACGGTCTCCTTGCGAATCCGAACCTCAAGATCTACAAGCCCTGGCTCGACACGCAGTTCATCTCGGAACTCGGCGGCCGCGCCGAAATGTCCGCCTTCCTTCAGGCCGAAGGCTTCAACTACCGCATGAGCGCCGAAAAGGCCTATTCGACCGACTCGAACATGCTCGGCGCCACGCACGAAGCGAAGGACCTCGAAGAGCTCTCCTCGAGCATCAAGATCGTCGAACCGATCATGGGCGTTCCCTTCTGGAAGGAAGACTGCGACGTCAAGCCCGAAGTCGTGACCGTGGCCTTTGAAGAAGGCATGCCGGTGGCGATCAACGGCCAGCGCTTCTCCGACCGCGTCGCGCTCATGGAAGAAGCCAACCGCGTGGGCGGCCGTCACGGTCTCGGCATGTCCGACCAGATCGAAAACCGCATCATCGAAGCGAAGAGCCGCGGCATCTACGAAGCCCCGGGGATGGCGCTTCTCTTCATCGCCTACGAACGTCTCGTCACCGGTATCCACAACGAAGACACGATCGAACAGTACCGCATCAACGGCATCAAGCTCGGTCGCCTCCTCTACCAGGGCCGCTGGTTCGACAGCCAGGCGATCATGCTGCGCGAAAGCGCCATGCACTGGGTGGCGCGTGCCGTCACGGGCGAAGTTACGCTCGAACTGCGCCGCGGCAACGACTACTCGATCCTCAACACCGTGAGCCCGAACCTTACGTACGAACCCTCGCGCCTCACGATGGAAAAGGGCGACTCGATGTTCACGGCCGTCGACCGTATCGGTCAGCTCACGATGCGCAACCTCGACATCGTCGACACGCGTCAGAAGCTCGGCATCTACTCGAAGGCCGGTCTCCTCGCCGCGGGTCCCGACGACGTCACCCCGATGCTCGGTCGCTAAGCCGCAAGGACGCAAACGCTCTTCGGAGCGTTGTTGAAGACTACGATTGAGAGCGCCGCCGGACGGAAAGCGATTTCCGTCCGGCGGCGTCTTTGCTTTGCGAACTCCCGGGGCCTGCGCGGCGGCAACCGCCGGGCAAGGGAGTAACATGCGCAAATGTCCCGTCGGACACGCGATGCCGACGAAAGAACCGACCCAACCATCCGAACAGAAGTACACACCATGAGCGAACGACACACGGACGGCGACATGCCGCAGACGGCTTCGCACGACTACACCGGTCACGGCCGGGGCGACAGTGCCTGGGGGCACGCCGCCGAAAAGACGAAAAAATCCGTCCTCGGCGTTGCGGTGGGCCTCACCCTCACTTTTTCCTTCATCGAACTCCTGGGCGGCCTCTGGAGCAATTCGCTCGCGCTGATCGGCGACGCGGGTCACATGGTGACCGACTCGGCGAGTCTTCTTTTCGCGCTGATCGCCAACCTGATCGCACAGCGCGGCGCCGACCGCGACCACTCGTTCGGGCACGGGCGCGTCGAAGTGCTCGCCGCCTTCGTGAACGGGATCGTCATGCTCGGCGTCGTCGTCTGGCTCTTTTGGGAGGCGTTCGGGCGCATCTCGAACCCCGAACCCGTTTCGGGCTTTTCGGTGATGGCCATTGCGGCGATCGGTCTCGTGATCAACCTCGGCGTTGCCTGGTCGCTTTCGCGCGACAAGAAAAACGTCAATACCCGCGCGGCGCTCCTGCACGTGATGGGGGACCTCTTGGGGTCGGTGGCCGCCATCGCGGCGGGCGCCGTGATCTGGATGGGCGGCCCCGCGATCATCGACCCGATTCTCTCGATGTTGGTGGGCGTGATGCTCTTGCACGCCACGTACGAAATTCTGCGCGACGCCTCGCGCGTGTTGCTCGACGGCGTTCCGGAAGGGGTGGAACTCGACGACGTGGGGCGCTTCCTCGAAGAGATTCCCTCGGTGCGGCACGTGCACGACTTGCACGTCTGGACGATGAGCCCGGGGCACGGAGCGATCCAGTGCCACGTGCAGATCGAGTCGCCCGCCTGCTGGCCGAAGATTCTCGATGCGATCCGCACGGGACTGCACGACCGCTTCGGGATCGACCACGTGACGGTGCAGCCCGAATGGGTGCTCGGCGAAACAGGGTGCGGCGACGCCTGCGACTGCACGTGCGAGGAGAAGGCGGGGTGCGACGGCGGTTGCGGCAAAGGAGCGCGCGGCTGCACGACGTGTTCGAGCGAACTCGTGGCGGCGCTTGACGAACGGATCGAGCGCGCCGAGCGCGATCTGCGCCTCAAAGAAGAAGCCGAGGAAGCCGGCCGAAGCGGCGACTGATTTGTTTCTTCCGGGGCTCTCGGCCCCGCTGCCTCCGTTTTTCCCGAAGCGCCCGGCCCTCGTGTCCGGGCGCTTTCTTTCGGGGCGGCGCGCCCCATTCGTCGGGCGCTTTCGAAGGGGGCTTGAACCTATCGGCTCCTCAGTGTTTTCTACTACAATGAGCACGCAGATTCGGACCCCTTTCGCGGGGTCCTTTTACGGATGTGGCCCTCGCGCCGCGGACCCCGGCCGGTCGGATGCGTTCCGCCCGGCCTCTCGGGCTTTTGCGTGCGGTTTGCGGCGAACATCGGGTTATAATCACCGTTTTTGATTTTTTCTTCACTTCTCGGCTGGACGGACAGTTTCATGACCAAGTATGTATTTGTGACGGGCGGCGTGGTTTCCTCCCTCGGTAAGGGCATTGCCGCGGCGTCCCTCGCTGCGATCCTCGAATCCCGCGGGCTCAAGGTGACGATGATCAAGCTCGATCCCTACATCAACGTGGACCCGGGTACGATGTCGCCTTTCCAGCACGGTGAAGTGTTCGTGACGGAAGACGGCGCCGAAACCGACCTCGACCTCGGGCACTACGAGCGCTTCATCTCCTCGAAGATGCACAAGCCCAACAACTTCACGTCGGGCCAGATCTACGAAAGCGTTCTTCGCAAGGAACGTCGCGGCGAATACCTCGGCAAGACCGTGCAGGTGATCCCGCACATCACGAACGAAATTCAGGAATTCATCGGCCGCGGCGCTTCCTCCGCCTGGGACGGCAAGCCCGACGTGTGCGTCGTTGAAATCGGCGGTACGGTGGGCGACATCGAATCGCTCCCCTTCGTCGAAGCCGTGCGTCAGATGTCCTTCCGCGTGGGCCGCAACAACACCTGCTTCATCCACCTCACGCTCTGCCCGTGGGTGGCCTCGGCGGGCGAATTGAAGACGAAGCCCACGCAGCACTCCGTGCAGAAGCTGCGCGAAGAAGGCGTCTATCCGGACGTGCTCCTTTGCCGTGCCGAACGCATGATCCCCGAAAACGAACGCGCGAAGATCTCGCTCTTCTCGAACGTTCCGATGAACTGCGTGATCAGCGTGGCCGACGCTTCGACGATCTACGAAGTGCCCCTGATGCTCCACGCCCAGAAGCTCGACGACATCGTCTGCGAAAAGCTCGGTCTCGAAACGCCGCCCGCCGACCTCTCGGCCTGGGAAAACATCGTCAACAACATCAAGCACCCGAAGCGCGAAGTGACGATCGGTATGGTCGGCAAGTACGTCGACTACGCCGACAGCTACAAGTCGCTCAACGAAGCGCTCGTGCACGCCGGCATCCACACGGAAACGAAGGTTCGCACCAAGTTCATCGACTCGACGAAGATCGAAGAAGAAGGTACGGCCGTGCTCGAAGGGCTTGACGCCATTCTCGTTCCGGGCGGCTTCGGCAAGCGCGGCACCGAAGGCATGATCAAGGCCATCGAGTTCGCCCGCGAAAAGAAGATCCCGTTCCTCGGCATCTGCCTCGGCATGCAGATGGCGGTGATCGAATTCGCCCGCCACGTCTGCGGTCTCGGCGGCGCCAACTCGACCGAACTCGACCCCGACACGCCGCATCCCGTCGTCGCGCTCATCACCGAGTGGAAGGACCGTACGGGCGAAATCCAGAAGCGCAGCGAAGGGTCGGACCTCGGCGGCACGATGCGTCTCGGTCGCCAGGAAGTGCCGGTTCGTCCGGGCACCCTCGCGCACCGCATCTACGGCGACGTCGTGGCCGAACGCCATCGTCACCGCTACGAAGTGAACAACGCCTACGTCGCGCAGTTCGAAGAAAAGGGCATGGTGATTTCGGCCAAGACGCCGAGCGAACACCTCCCCGAAATGATGGAGTTGCCCGATCATCCCTTCTTCTTCGCCGTGCAGTTCCATCCGGAATTCACGTCGAATCCGCGCTTCGGTCATCCGCTCTTCAAGGCGTATGTCGAAGCGGCCATCGCCCGCTCCGAAGCGAAGCAGGCGAACTGACCGTAAGGTCGAATCGGGACGGAGCCGCGGCTTCGTCCCGAACACACGAATGTTGCGCCGACCCGATCACCCAACCGCCCGCCTAGCGCGAACGGGGGCGAGGGGTCGGTTTTTTTTCGAGGAGAATTTGCCGATGCAGCTGTGTGGTTTCGAAGCCGGGCTTGACCGCCCCTTCTTTCTTTTGGCGGGCCCCTGCGTGCTCGAAGGCGAACAGATGGCGATCGACATCGCCGGGCATCTCAAGGAGGTGACGGGCGAACTCGGCATCCCCTACGTTTTCAAGGCGAGCTACGACAAGGCGAACCGCACCTCCGGGAAGAGCTTCCGCGGTCCCGGCATGGAAGAGGGGCTGCGCATCCTCGAGGAAGTGCGTCGTCAGGTGGGAGTGCCCGTCATCACGGACGTGCATACCGAGGCCGAAGTTCCTGCGGTTGCGGCCGTGGCGGATATTCTGCAGACACCCGCGTTCCTCTGCCGTCAGACGGACTTCATCCGCGCCTGCGCAATGTCGGGCAAACCCGTCAACATCAAGAAGGGTCAGTTCCTCGCCCCGGGCGACATGAAGAACGTCGTTGAAAAGGCGCGTGCCGCCGCCGAAGAAGCGGGTCTTTCGACCGATCGCTTCCTCGTGTGCGAACGCGGCGCCTCCTTCGGCTACGGGAACCTCGTCTCCGACATGCGCTCGCTTGCGATCATGCGCGAAACGGGCGCCCCCGTCGTCTTCGACGCCACGCACTCCGTGCAGCTCCCCGGCGGCAACGGCACTTGCTCGGGCGGCCAGCGCCAGTTCGTGCCCGTCCTCGCGCGTGCGGCCGTTGCCGTCGGCGTTGCGGGTCTCTTCATGGAAACGCACCCGAACCCCGCCTGCGCGCTGAGCGACGGCCCGAACGCCGTTCCGCTCGACCGAATGAAGGATCTCCTCACGAGCCTCGTTGAAATCGACCGCATCGTGAAGGGCCGTCCCTTCCTCGAAGACACCTTCGCTCTCTAACATCGTTTCGAGCGACGCCTCTTTCGGGACTTCCGGTCGCGACGTCGCTCCACCTCACGCTCCAACCCAGAAAAGGATTCATCATGAGTTCCATTATTGATGTCATCGGCCGTGAAGTTCTCGACAGCCGCGGCAATCCCACCGTCGAAGCCGAAGTCTGGCTCGAAAGCGGCGCCGTCGGCCGCGCGGCCGTTCCCTCGGGCGCCTCGACGGGCGTGCGCGAAGCGATCGAACTGCGCGACAACGATCCGAAGCGCTACTGCGGCAAGGGCGTGCTCACCGCCGTGAGTCACGTTTCGGGTGAAATCGCCGACGCGCTGATCGGTCTCGAAGCCACCGACCAGGCCTACATCGATCGCCTCATGGTCAAGCTCGACGGCACGGACAACAAGAGCCGTCTCGGCGCCAACGCGATCCTCGCCGTCTCGATGGCCGTCGCCCGCGCCGCGGCCGAAGAGTCCGGCCAACCCCTCTACCGCTACTTCGGCGGCATGGGCGCTCTTCAGATGCCCGTCCCGATGATGAACGTCATCAACGGCGGCGCGCACGCGAACAACAACCTCGACCTTCAGGAATTCATGATCATTCCGGTGGGTGCTCCGTCGCTGCGCGAAGCGCTTCGCTACGGCGCCGAAACCTTCCACGCCCTGAAGAAGATCATCAACGGTCGCGGCATGTCGACCGCCGTGGGCGACGAAGGCGGTTTCGCTCCCAAGTGCGAATCGCACGAAGAAGCGATCGAACTCATCCTCGAAGCCATCCGCGCCGCGGGCTACGAACCGGGCAAGGACATCGCGCTCGCGCTCGACTGCGCTTCGTCCGAATTCTTCGTCGACGGCAAGTACGTCATGAAGAAGTCCTCGGGCGCCGCGCTCACCGCCGAAGAATGGATCGAAGTGCTCGCCGGCTGGTGCGACAAGTACCCGATCATCTCGATCGAAGACGGCATGGCCGAAGGCGACTGGGAAGGCTGGGCCAAGCTCACGGCCGCTCTCGGCGAACGCGTGCAGCTCGTCGGCGACGACCTCTTCGTCACGAACCCCGCGATCCTCAAGGAAGGCATCGAAAAGGGCGTGGCCAACTCGATCCTCATCAAGGTCAACCAGATCGGCACCCTCACGGAAACGTTCGAAGCGATCGAAATGGCCAAGCGCGCCGGCTACACGGCCGTCATCTCGCACCGCTCGGGCGAAACGGAAGACAGCACGATCGCCGACATCGCCGTCGGTCTCAACGCCGGCCAGATCAAGACGGGCTCGATGAGCCGCTCCGACCGTATGGCCAAGTACAACCAGCTCCTTCGCATCGAAGAGCACCTCGCCGAAGTGGCCGTCTATCCCGGCCGCGACGCGTTCTACTCGATCCGTCGCTGAGTCGATGCGCGTTGAGCGGAGAAGACACCTCCCATGATCCGTATCTTCATCGTCCTGCTTCTTCTCGGCATCGGCGTCACGCAGTACCAGCTTTGGGCCGGTCGCGCAAGCTGGTTCCGATTGAAGGAACTCGAGCAGACGCTCGTGGCCCAACGGGCCGCGAACGATCGGCTGCGCCTGCAAAACGAAGCGTTTGCGGCCGAACTCTACTCGCTTGAAAACCAGCGCGACGCTATCGAAGAGCGCGCACGCTGGGATTTGACCATGGTGAAGGATCGCGAAGTGCTTTTCCGCCTCGAAGGCGAAGAGGCGGCCCGGGATCGGGTCGCCGCGAGCATGCCCGACTATCAGCAGCCCGATATCAAGCCGGGGTCCGATCCCACGTTCGAAGCGAAGAAGTCGGACCTCTACCACGCGCCGAAGCACCTCTCGGCGCCGAAGGCGCGGGACCGCCGCGAGTGATTTCGGGCCCCCGGGCGACGCGCTCGACAACGAAAAACGGCATGCCTTTTGCGAAGGGCATGCCGTTTTTCTTTGAGGTCGATGCGCGCTTTACGGGGTTTCGGTCGATTCGCGAGGAAGTTCGACGAGGACGCTCGCCTTGAGCCCCGGAGGAACCTCGCCCGGACGGGCGTCGGCGAGATCGATCCGCGCTCCCACCATCTCGGCGTAGGTGCGTACGATCGCAAGCCCCAGGCCCGTTCCCGGAAGTCCCGAACCCGCAATGCGGTAGAAGGGGTCGAAAACGCGTTCGCGTTCGCTCTCGGGAATGCCCGGACCCGTATCCGAAACAACGATCGAAAGCTTCCCGGCCGAACGATCGATTCTCACCGTCACGGCCCCTCGGGCGGGCGTGTAGTGAACGGCGTTTTCGACGAGGTTGCGCATCATCGCCTGAATCGCTGCGCGCGAGACGCCCTTGACGGGCGCTTCGGGCGTGTCGGCATCGAGCCCCTCGACTTCGAAGCCGATCGACTTCTCGGCGATGGCGGGGAGGATCGGCTCCACCACTTCGCCTACGAGCTCCGAGAGGTACCAGGGTTTGGCGTCGCGCGCAAGCACGGCGGCGCTCTCGCCCGCCTGGGCGCGCGCAAAGAGAAGGAGCTGCGACACCTGATGGACCGACCGCGCAAGGCCCGACTCCAGGTTTTCGACGATCGGGCGCGCTTCGTCGGAGAGCTTGAGACGCTTGAGGTGCTCGGCCTCGATCGTGAGCGACGTAAGCGGCGATCGCAGTTCGTGCGCGGCATCCGCCGTAAAGCGGATTTCGCGCGTGCGCGCGGCCTGCACGCGCGCGAGTAGGTCGTTCACGGCTTCGACGAAGGGGGCGATTTCCAAGGGAACGCCCTCGTAGGAGAGGGGCGCGAGGTCCGTGGCCGAGCGCCCCTTCACGTCGGCAGCGGCGCGGTTCAAAGGCCGCAGCGCCCGCCAAAGGACGGCACTGATCGCAAAAAGAAGGAGAGGCAGCAAAACAAGAAGCGGCATCACCGCGGTCTTCGCCTGCTCCAGCACCGTTGCTTCGCGCATCGCCATCGGTTCGGCGAGCGCCGTGTAGCGGCCGTTGGGCAAAAAGACGAGGCATACGCGGTGCGGCTCGCCGTTGATCGTGAGCGTGGAAAGTCCTGCGGGGAGGGCGTGCTCCAACGTGGTCGACACCGCCTGCCCCTGACGGGTAATAAGCCGAACGAGCACGGATTCGCCCGCGGGAATGGCCGTGCGGCGCTCGTTCGGGGTCGTTTCCCGATGCATGCCCATGCGGTTCATCATCCCGCGCATCATCATGCGGTGGTGGCGGCTCGGGAGCGGTTCGTCCGATTCGATCCGCTCGGCAAACTGTTGCGGGTCCATCGTGAGCGCCCGGGGGACGAGCGCCGCTACGTCCGCACGGGCGAGCGCGGCCGTCATGTCCGCAAGATGCGCGTCCTGCATGGCGAGCGCGTCTTCGCGGGCCCACCAAAAGAGAGCGGCCGCGGTGAGCATCATCAAGAGGGTCGATACCGTGACGACGGCGGCCAGGGTCCGTCCGAGAAGGGAGCGCCTCAGAGTCGAGAGAAACAT

Protein sequences of DBSCAN-SWA_1

>AP018786|733379:742478|738189_739047_+|BBF22735.1|DBSCAN-SWA
MQLCGFEAGLDRPFFLLAGPCVLEGEQMAIDIAGHLKEVTGELGIPYVFKASYDKANRTSGKSFRGPGMEEGLRILEEVRRQVGVPVITDVHTEAEVPAVAAVADILQTPAFLCRQTDFIRACAMSGKPVNIKKGQFLAPGDMKNVVEKARAAAEEAGLSTDRFLVCERGASFGYGNLVSDMRSLAIMRETGAPVVFDATHSVQLPGGNGTCSGGQRQFVPVLARAAVAVGVAGLFMETHPNPACALSDGPNAVPLDRMKDLLTSLVEIDRIVKGRPFLEDTFAL
>AP018786|733379:742478|740453_740882_+|BBF22737.1|DBSCAN-SWA
MIRIFIVLLLLGIGVTQYQLWAGRASWFRLKELEQTLVAQRAANDRLRLQNEAFAAELYSLENQRDAIEERARWDLTMVKDREVLFRLEGEEAARDRVAASMPDYQQPDIKPGSDPTFEAKKSDLYHAPKHLSAPKARDRRE
>AP018786|733379:742478|734930_736061_+|BBF22733.1|DBSCAN-SWA
MSERHTDGDMPQTASHDYTGHGRGDSAWGHAAEKTKKSVLGVAVGLTLTFSFIELLGGLWSNSLALIGDAGHMVTDSASLLFALIANLIAQRGADRDHSFGHGRVEVLAAFVNGIVMLGVVVWLFWEAFGRISNPEPVSGFSVMAIAAIGLVINLGVAWSLSRDKKNVNTRAALLHVMGDLLGSVAAIAAGAVIWMGGPAIIDPILSMLVGVMLLHATYEILRDASRVLLDGVPEGVELDDVGRFLEEIPSVRHVHDLHVWTMSPGHGAIQCHVQIESPACWPKILDAIRTGLHDRFGIDHVTVQPEWVLGETGCGDACDCTCEEKAGCDGGCGKGARGCTTCSSELVAALDERIERAERDLRLKEEAEEAGRSGD
>AP018786|733379:742478|739130_740420_+|BBF22736.1|DBSCAN-SWA
MSSIIDVIGREVLDSRGNPTVEAEVWLESGAVGRAAVPSGASTGVREAIELRDNDPKRYCGKGVLTAVSHVSGEIADALIGLEATDQAYIDRLMVKLDGTDNKSRLGANAILAVSMAVARAAAEESGQPLYRYFGGMGALQMPVPMMNVINGGAHANNNLDLQEFMIIPVGAPSLREALRYGAETFHALKKIINGRGMSTAVGDEGGFAPKCESHEEAIELILEAIRAAGYEPGKDIALALDCASSEFFVDGKYVMKKSSGAALTAEEWIEVLAGWCDKYPIISIEDGMAEGDWEGWAKLTAALGERVQLVGDDLFVTNPAILKEGIEKGVANSILIKVNQIGTLTETFEAIEMAKRAGYTAVISHRSGETEDSTIADIAVGLNAGQIKTGSMSRSDRMAKYNQLLRIEEHLAEVAVYPGRDAFYSIRR
>AP018786|733379:742478|733379_734708_+|BBF22732.1|DBSCAN-SWA
MSQTILQSVPVGQKVGIAFSGGLDTSAALRWMKNKGALPYAYTANLGQPDEKDYEAIPRRAMEYGAENARLIDCRPQLVNEGIAAIQSGAFHISTGGQLYFNTTPIGRAVTGTMLVAAMREDGVHIWGDGSTYKGNDIERFYRYGLLANPNLKIYKPWLDTQFISELGGRAEMSAFLQAEGFNYRMSAEKAYSTDSNMLGATHEAKDLEELSSSIKIVEPIMGVPFWKEDCDVKPEVVTVAFEEGMPVAINGQRFSDRVALMEEANRVGGRHGLGMSDQIENRIIEAKSRGIYEAPGMALLFIAYERLVTGIHNEDTIEQYRINGIKLGRLLYQGRWFDSQAIMLRESAMHWVARAVTGEVTLELRRGNDYSILNTVSPNLTYEPSRLTMEKGDSMFTAVDRIGQLTMRNLDIVDTRQKLGIYSKAGLLAAGPDDVTPMLGR
>AP018786|733379:742478|736406_738056_+|BBF22734.1|DBSCAN-SWA
MTKYVFVTGGVVSSLGKGIAAASLAAILESRGLKVTMIKLDPYINVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFISSKMHKPNNFTSGQIYESVLRKERRGEYLGKTVQVIPHITNEIQEFIGRGASSAWDGKPDVCVVEIGGTVGDIESLPFVEAVRQMSFRVGRNNTCFIHLTLCPWVASAGELKTKPTQHSVQKLREEGVYPDVLLCRAERMIPENERAKISLFSNVPMNCVISVADASTIYEVPLMLHAQKLDDIVCEKLGLETPPADLSAWENIVNNIKHPKREVTIGMVGKYVDYADSYKSLNEALVHAGIHTETKVRTKFIDSTKIEEEGTAVLEGLDAILVPGGFGKRGTEGMIKAIEFAREKKIPFLGICLGMQMAVIEFARHVCGLGGANSTELDPDTPHPVVALITEWKDRTGEIQKRSEGSDLGGTMRLGRQEVPVRPGTLAHRIYGDVVAERHRHRYEVNNAYVAQFEEKGMVISAKTPSEHLPEMMELPDHPFFFAVQFHPEFTSNPRFGHPLFKAYVEAAIARSEAKQAN
>AP018786|733379:742478|740972_742478_-|BBF22738.1|DBSCAN-SWA
MFLSTLRRSLLGRTLAAVVTVSTLLMMLTAAALFWWAREDALAMQDAHLADMTAALARADVAALVPRALTMDPQQFAERIESDEPLPSRHHRMMMRGMMNRMGMHRETTPNERRTAIPAGESVLVRLITRQGQAVSTTLEHALPAGLSTLTINGEPHRVCLVFLPNGRYTALAEPMAMREATVLEQAKTAVMPLLVLLPLLLFAISAVLWRALRPLNRAAADVKGRSATDLAPLSYEGVPLEIAPFVEAVNDLLARVQAARTREIRFTADAAHELRSPLTSLTIEAEHLKRLKLSDEARPIVENLESGLARSVHQVSQLLLFARAQAGESAAVLARDAKPWYLSELVGEVVEPILPAIAEKSIGFEVEGLDADTPEAPVKGVSRAAIQAMMRNLVENAVHYTPARGAVTVRIDRSAGKLSIVVSDTGPGIPESERERVFDPFYRIAGSGLPGTGLGLAIVRTYAEMVGARIDLADARPGEVPPGLKASVLVELPRESTETP

Homologous phage analysis in the prophage region

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
BBF22732.1|733379_734708_+	argininosuccinate synthase	A0A140XAJ5	Dickeya_phage	7.8e-51	77.8
BBF22733.1|734930_736061_+	hypothetical protein	A0A1V0SED0	Indivirus	2.6e-07	24.4
BBF22734.1|736406_738056_+	CTP synthase	A0A1J0FA14	Only_Syngen_Nebraska_virus	4.7e-154	52.6
BBF22735.1|738189_739047_+	2-dehydro-3-deoxyphosphooctonate aldolase 1	E3T537	Cafeteria_roenbergensis_virus	3.7e-46	39.9
BBF22736.1|739130_740420_+	enolase	A0A1X9I5Z8	Streptococcus_phage	1.1e-150	64.1
BBF22737.1|740453_740882_+	hypothetical protein	NA	NA	NA	NA
BBF22738.1|740972_742478_-	hypothetical protein	A0A2K9L0Z8	Tupanvirus	4.3e-05	33.0