CRISPRimmunity

Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Self targeting spacer number	Target MGE spacer number	Prophage number	Anti-CRISPR protein number
CP027772	Halobacteriovorax sp. BALOs_7 chromosome, complete genome	3 crisprs	cas3,csa3,DEDDh,DinG,WYL	0	3	0	0

1. CP027772

CRISPR-Cas detection and classification

Crispr_ID: CP027772_2

• CRISPR_ID: CP027772_2
• CRISPR_location: 1266659-1266766
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 1 spacers

Consensus repeat of CP027772_2

Consensus_repeat	Method
GCTTATTTTAGCTGCGCTAAAATGTCGCTTTT	CRISPRCasFinder

spacers of CP027772_2

>2.1|1266691|44|CP027772|CRISPRCasFinder
ATTGGTGCCATGCTTTTTTTATTTCTCATGTGCTAAGGGGGGGG

CRISPR arrays and Neighbor proteins around CP027772_2

CRISPR arrays

The CRISPR arrays of CP027772_2

>merge|CP027772|2|1266659-1266766|CRISPRCasFinder
GCTTATTTTAGCTGCGCTAAAATGTCGCTTTTATTGGTGCCATGCTTTTTTTATTTCTCATGTGCTAAGGGGGGGGGCTTATTTTAGCTGCGCTAAAATATCGCTTTT

>CP027772|2|1|1266659-1266766|CRISPRCasFinder
GCTTATTTTAGCTGCGCTAAAATGTCGCTTTT	ATTGGTGCCATGCTTTTTTTATTTCTCATGTGCTAAGGGGGGGG
GCTTATTTTAGCTGCGCTAAAATATCGCTTTT

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
CP027772.1|AYF44266.1|1270778_1272212_-|FeS-assembly-protein-SufB	unknown	unknown	gnl|CDD|236990
CP027772.1|AYF44258.1|1264277_1265177_+|coproporphyrinogen-III-oxidase,-aerobic	unknown	unknown	gnl|CDD|376494
CP027772.1|AYF44253.1|1255107_1258251_+|hypothetical-protein	unknown	unknown	unknown
CP027772.1|AYF44269.1|1273659_1274919_-|pseudouridylate-synthase	unknown	unknown	gnl|CDD|211334
CP027772.1|AYF44270.1|1274943_1275561_+|PF06080-family-protein	unknown	unknown	gnl|CDD|253548
CP027772.1|AYF44256.1|1262274_1263654_-|sigma-factor-regulatory-protein,-FecR/PupR-family	unknown	unknown	gnl|CDD|377406
CP027772.1|AYF44259.1|1265177_1265369_-|hypothetical-protein	unknown	unknown	unknown
CP027772.1|AYF44263.1|1267496_1268717_-|cysteine-desulfurase,-SufS-family	unknown	unknown	gnl|CDD|99746
CP027772.1|AYF44254.1|1258282_1260559_-|CHASE2-domain-protein	unknown	unknown	gnl|CDD|143636
CP027772.1|AYF44255.1|1260570_1262142_-|hypothetical-protein	unknown	unknown	gnl|CDD|276809
CP027772.1|AYF44261.1|1265873_1266461_+|channel-protein,-hemolysin-III-family	unknown	unknown	gnl|CDD|224191
CP027772.1|AYF44257.1|1263827_1264190_-|putative-arsenate-reductase	unknown	unknown	gnl|CDD|239332
CP027772.1|AYF44264.1|1268716_1269967_-|FeS-assembly-protein-SufD	unknown	unknown	gnl|CDD|273908
CP027772.1|AYF44267.1|1272204_1272639_-|transcriptional-regulator	unknown	unknown	gnl|CDD|224870
CP027772.1|AYF44262.1|1266908_1267472_-|scaffold-protein-Nfu/NifU-N-terminal-domain-protein	unknown	unknown	gnl|CDD|376458
CP027772.1|AYF44265.1|1269995_1270754_-|FeS-assembly-ATPase-SufC	unknown	unknown	gnl|CDD|273907
CP027772.1|AYF44260.1|1265501_1265729_+|hypothetical-protein	unknown	unknown	unknown
CP027772.1|AYF44268.1|1272827_1273697_+|peptidase,-S54-family	unknown	unknown	gnl|CDD|275075
CP027772.1|AYF44252.1|1252871_1254923_+|dehydrogenase-E1-component	unknown	unknown	gnl|CDD|223101
CP027772.1|AYF44271.1|1275566_1277333_-|arginine--tRNA-ligase	unknown	unknown	gnl|CDD|223097

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
CP027772.1|AYF44258.1|1264277_1265177_+|coproporphyrinogen-III-oxidase,-aerobic	gnl|CDD|376494	pfam01218, Coprogen_oxidas, Coproporphyrinogen III oxidase.	1.93778e-125
CP027772.1|AYF44264.1|1268716_1269967_-|FeS-assembly-protein-SufD	gnl|CDD|273908	TIGR01981, UPF0051_protein_Rv1462/MT1509, FeS assembly protein SufD. This protein, SufD, forms a cytosolic complex SufBCD. This complex enhances the cysteine desulfurase of SufSE. The system, together with SufA, is believed to act in iron-sulfur cluster formation during oxidative stress. SufB and SufD are homologous. Note that SufC belongs to the family of ABC transporter ATP binding proteins, so this protein, encoded by an adjacent gene, has often been annotated as a transporter component. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other].	1.65727e-77
CP027772.1|AYF44269.1|1273659_1274919_-|pseudouridylate-synthase	gnl|CDD|211334	cd02566, PseudoU_synth_RluE, Pseudouridine synthase, Escherichia coli RluE. This group is comprised of bacterial proteins similar to E. coli RluE. Pseudouridine synthases catalyze the isomerization of specific uridines in an RNA molecule to pseudouridines (5-ribosyluracil, psi). No cofactors are required. Escherichia coli RluE makes psi2457 in 23S RNA. psi2457 is not universally conserved.	4.54574e-75
CP027772.1|AYF44270.1|1274943_1275561_+|PF06080-family-protein	gnl|CDD|253548	pfam06080, DUF938, Protein of unknown function (DUF938). This family consists of several hypothetical proteins from both prokaryotes and eukaryotes. The function of this family is unknown.	8.00074e-45
CP027772.1|AYF44256.1|1262274_1263654_-|sigma-factor-regulatory-protein,-FecR/PupR-family	gnl|CDD|377406	pfam04773, FecR, FecR protein. FecR is involved in regulation of iron dicitrate transport. In the absence of citrate FecR inactivates FecI. FecR is probably a sensor that recognizes iron dicitrate in the periplasm.	4.00689e-14
CP027772.1|AYF44263.1|1267496_1268717_-|cysteine-desulfurase,-SufS-family	gnl|CDD|99746	cd06453, SufS_like, Cysteine desulfurase (SufS)-like. This family belongs to the pyridoxal phosphate (PLP)-dependent aspartate aminotransferase superfamily (fold I). The major groups in this CD correspond to cysteine desulfurase (SufS) and selenocysteine lyase. SufS catalyzes the removal of elemental sulfur and selenium atoms from L-cysteine, L-cystine, L-selenocysteine, and L-selenocystine to produce L-alanine; and selenocysteine lyase catalyzes the decomposition of L-selenocysteine.	0
CP027772.1|AYF44254.1|1258282_1260559_-|CHASE2-domain-protein	gnl|CDD|143636	cd07302, CHD, cyclase homology domain. Catalytic domains of the mononucleotidyl cyclases (MNC's), also called cyclase homology domains (CHDs), are part of the class III nucleotidyl cyclases. This class includes eukaryotic and prokaryotic adenylate cyclases (AC's) and guanylate cyclases (GC's). They seem to share a common catalytic mechanism in their requirement for two magnesium ions to bind the polyphosphate moiety of the nucleotide.	1.80664e-57
CP027772.1|AYF44255.1|1260570_1262142_-|hypothetical-protein	gnl|CDD|276809	sd00006, TPR, Tetratricopeptide repeat. The Tetratricopeptide repeat (TPR) typically contains 34 amino acids and is found in a variety of organisms including bacteria, cyanobacteria, yeast, fungi, plants, and humans. It is present in a variety of proteins including those involved in chaperone, cell-cycle, transcription, and protein transport complexes. The number of TPR motifs varies among proteins. Those containing 5-6 tandem repeats generate a right-handed helical structure with an amphipathic channel that is thought to accommodate an alpha-helix of a target protein. It has been proposed that TPR proteins preferentially interact with WD-40 repeat proteins, but in many instances several TPR-proteins seem to aggregate to multi-protein complexes.	2.21107e-09
CP027772.1|AYF44266.1|1270778_1272212_-|FeS-assembly-protein-SufB	gnl|CDD|236990	PRK11814, PRK11814, cysteine desulfurase activator complex subunit SufB; Provisional.	0
CP027772.1|AYF44257.1|1263827_1264190_-|putative-arsenate-reductase	gnl|CDD|239332	cd03034, ArsC_ArsC, Arsenate Reductase (ArsC) family, ArsC subfamily; arsenic reductases similar to that encoded by arsC on the R733 plasmid of Escherichia coli. E. coli ArsC catalyzes the reduction of arsenate [As(V)] to arsenite [As(III)], the first step in the detoxification of arsenic, using reducing equivalents derived from glutathione (GSH) via glutaredoxin (GRX). ArsC contains a single catalytic cysteine, within a thioredoxin fold, that forms a covalent thiolate-As(V) intermediate, which is reduced by GRX through a mixed GSH-arsenate intermediate. This family of predominantly bacterial enzymes is unrelated to two other families of arsenate reductases which show similarity to low-molecular-weight acid phosphatases and phosphotyrosyl phosphatases.	1.6007e-36
CP027772.1|AYF44265.1|1269995_1270754_-|FeS-assembly-ATPase-SufC	gnl|CDD|273907	TIGR01978, Probable_ATP-dependent_transporter_SufC, FeS assembly ATPase SufC. SufC is part of the SUF system, shown in E. coli to consist of six proteins and believed to act in Fe-S cluster formation during oxidative stress. SufC forms a complex with SufB and SufD. SufC belongs to the ATP-binding cassette transporter family (pfam00005) but is no longer thought to be part of a transporter. The complex is reported as cytosolic () or associated with the membrane (). The SUF system also includes a cysteine desulfurase (SufS, enhanced by SufE) and a probable iron-sulfur cluster assembly scaffold protein, SufA. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other].	2.27934e-133
CP027772.1|AYF44267.1|1272204_1272639_-|transcriptional-regulator	gnl|CDD|224870	COG1959, COG1959, Predicted transcriptional regulator [Transcription].	5.64944e-21
CP027772.1|AYF44262.1|1266908_1267472_-|scaffold-protein-Nfu/NifU-N-terminal-domain-protein	gnl|CDD|376458	pfam01106, NifU, NifU-like domain. This is an alignment of the carboxy-terminal domain. This is the only common region between the NifU protein from nitrogen-fixing bacteria and rhodobacterial species. The biochemical function of NifU is unknown.	4.9159e-24
CP027772.1|AYF44261.1|1265873_1266461_+|channel-protein,-hemolysin-III-family	gnl|CDD|224191	COG1272, COG1272, Predicted membrane protein, hemolysin III homolog [General function prediction only].	3.27365e-47
CP027772.1|AYF44268.1|1272827_1273697_+|peptidase,-S54-family	gnl|CDD|275075	TIGR04239, GlpG_protein, rhomboid family protease GlpG. GlpG in E. coli is a rhomboid family intramembrane serine protease that has been extensively characterized as a proxy for rhomboid family proteases in animals. It efficiently cleaves eukaryote-derived model substrates. This multiple membrane-spanning protein excludes inappropriate substrates from access to its cleavage site, and shows activity against truncated versions, but not full-length versions, of the E. coli multidrug transporter MdfA. This finding suggests a housekeeping function in removing faulty proteins. In contrast, several eukaryotic rhomboid family proteases release peptide hormones for signaling functions, and the Shewanella and Vibrio protein rhombosortase appears to be part of a protein-sorting system, cleaving a C-terminal anchoring helix domain.	1.01686e-53
CP027772.1|AYF44252.1|1252871_1254923_+|dehydrogenase-E1-component	gnl|CDD|223101	COG0022, AcoB, Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit [Energy production and conversion].	8.43595e-121
CP027772.1|AYF44271.1|1275566_1277333_-|arginine--tRNA-ligase	gnl|CDD|223097	COG0018, ArgS, Arginyl-tRNA synthetase [Translation, ribosomal structure and biogenesis].	2.0079e-131

Protein sequences

>CP027772.1|AYF44261.1|1265873_1266461_+|channel-protein,-hemolysin-III-family
MFFVSLGACSLLIAKSANTTQYISTIVYSIGLLMMLGVSAFYHRIHWEPRKRDLLGRLDHSAIYVMIAGTCTPITLLVLNGESGSTLLISIWTVAAIGILKSIFFPNLPEKVSALIYLIPGYMVLPYVSELMPKLGVSNIVLLITGGVLYTIGALFYGLKMPGRNAKWFGYHEIFHLFVCVAAVLHFIIIYSIVG
>CP027772.1|AYF44260.1|1265501_1265729_+|hypothetical-protein
MSKKLQELLADLENFSPKQMRTLRNNLNNRLQSFSNEYKEPKELQPSHKLYGLEEGECRELLGVVKKKLIQAKFS
>CP027772.1|AYF44259.1|1265177_1265369_-|hypothetical-protein
MPNLYLRDLTGAKDMMINNCYRLLCEITKLEQKIEEVQSLAKTHGLEIDTDKLLQELINKQAS
>CP027772.1|AYF44258.1|1264277_1265177_+|coproporphyrinogen-III-oxidase,-aerobic
MSFDIENIKNEFAAHVQDLQNQISEELKRIDTKINLNEDIWERKDFAGNPGGGGITRAFEGEIFENAGVNTSLVFGEVDPKFAANIGGDNNQMWATGISLIIHPTNPRVPTTHANFRMIQAGSKVWFGGGADLTPFYPHEDDFEYFHKVWKDACEPYGHWDEWKEKCDNYFVNKHRADEMRGIGGFFYDHFFSGDPVKDLAMFKEISSNFIKSYFPIVEKRMDEEFDDEDVDFQLHRRGRYVEFNLLHDRGTHFGLKSNGRTDSILISLPKRVKFTYRYEPKKPEHKKMMEYYFPKDWA
>CP027772.1|AYF44257.1|1263827_1264190_-|putative-arsenate-reductase
MLYYHNPRCSKSRKGLEYLESKGIQEGVGFRVKEYLKDGVEEKEFLDLIKYTGLAPLDGLIRTKEALFKDLGLAGKELSDMEWAKLVHENPKLLERPILVNDDKKAAIGRPDAHSFDPII
>CP027772.1|AYF44256.1|1262274_1263654_-|sigma-factor-regulatory-protein,-FecR/PupR-family
MKLLKQFIIFQIALVLNLSILNAAGDKGVAKVMKKRGKVFITQTKKKLKKGDWVPEGASITTKSRSFVKLLFIDKSNLTLGANSQMEIKQFPKKKAGIINLVNGQLRSKVSKNYLQIDKKKSKLFIKTKTAAMGVRGTDFQVNYNSKNQNTTLITFEGAVAMGSLSKFKVSDFRQDRLEKVVSSPTSVMVKRGQLSGVMPAIDSKPIAPVKINKKQLKALENNDGSKMTNAKDKKNKKAKPMARNIIPPGVDSKSFSASTNTEVADQMAKVDSSIRGPASEAKKKKKIQISQDTPKGSLKDGGYVDTENALYIPPPKNAAMDPMTNEVIVPVQMGSFDKETGMYTNDYYEVDESGNFIEKEDDSLTRLPASTSTMDTTNSDMKEVTNVYDVMDEQMDDTMKMDDTTISLTDPNRTTASTEEDKMMEDTMSDRDDMIETTKDPLSSTNRAVIRVILEKQP
>CP027772.1|AYF44255.1|1260570_1262142_-|hypothetical-protein
MKKLLLLSLLSCSLFAQGLQSIYQEFENANYEDVIEKLNEEQYKKRQDQKYLGVRSYLLGVSHARLQDFELAIKYFKDALKNKNDAKEIYYELGQAQYAINQLDDSINNFKNSIKIKYKVEQSTYYIAYINQLKEDLPQAKKYYKLLLKNKDTSIEMKQIAYYQVALINLDIAKGTNRASEIVEKYILPDLYRAINILPNSSTADDIDKRISDIRKEFYLEEDKLVNGAPLPSQRLNLGVSEKIAYDNNVTRSDDLPSSTGTNKDSFYSQTRFNISKAYNFKRRYTLTPRFELTSTTYSDRNDTAIFSNDSYNITPEVDFSFAYKYKKKPARFFLNYTYDYQARDINSEKDRQYTYRSNQFSLGTELPHFEIGNTSISIKSQSFSYYNDSLNRSSTIFSIDQIIKKNYGIFILLYQYDMSDYENNETLSTNSHLFRLDYLYPGIFPNTTLNIGLADMLISYDDVSQDAARGLESNISFNTKFIRVINRNLSFNVEYLYTNNISDEDASNYTAHQTSFELNYDY
>CP027772.1|AYF44254.1|1258282_1260559_-|CHASE2-domain-protein
MKNIVKNIGLFIIIIFSCVSVFFSLYSKDYEAKWRDLTSYASFFENRFYDFRMLQTMHKRKPHPKIILADIDDDSIKKLNSWPIPRAKWAEMLKKLRGYGAKIVAFDVFFSEDSMTCNGQNPDDDLVAAIEYFQEVPNNRVILPYNLQVGGTEHYTSIPDDLYIFALTTKVGGANYEMYPTFISDSVYPLQKLIDAETSLGFIEATADTDGVIRHYKALAFPYGEVHVPSYGLTAYMHWIGEFDEYDDPSKMDKIFKTQLDLSTAGGKPILKTKKGDIELNLKGETKVRWTGGEETYKRIPIYDIITKDPNDEELKNLFEDALVFVGATAYGAHDLRNTPVHPMLPGVMVHMNFAQMLIDGYYFKPFKESAKLSWLLLIISVIILLLVQLKENPIYDLAILVVLVTVLFYADTYYFVEQGYETKLFWCFFALTMCYGWNTIVNFYMANQDKAFLKNAFGNYISPELIDEMYESGHAPTLGGTSGIRTAYFTDIQGFSSFSEKLSATQLVELLNEYLTAMTDILLEEKGTLDKYEGDAIIAFFGAPMELPDHAQRSCMVAHRMQEKLLELRKKWVSEGDKWPKIVHDMRMRIGINSGDIVTGNMGSASRMNYTMMGDSVNLAARLEESAKQYGIFTQVAKETVEMAGDKFLWRELDTIKVVGKSIPVTTYDLLGLKETAPDYLKELSEKFSAAILLYKAQKWDEAIELFKVTLELEYMRFPDLKGVKTNPSEIYIKRCEDYKKLPPPPEWDGVFTLTSK
>CP027772.1|AYF44253.1|1255107_1258251_+|hypothetical-protein
MKIISIIAILLLISCGDKNTYQVNEVSTISNDNENTQSSEDNNRSSQDDVQLSTSVLAPSAIPFIGKRFSIKNTSDLKYVDFSYQRVSDTSQFNNIEFKEGPRYAKLNGIKLVINEGNRLYTYLANNLDIKILEINAEEVVINSKIWLPSTNVTINAKKLIIGPLGSLSTTPTFLPDNANSFEDGRKGLKAGDIKLNISKLDIRGHERVIFNLVGGKGQDAGPGKDGRNGSIPGLNDLGDGVVYKQYATQIVEDINPRNPNRFFTIPVSVRVDKEIGKKQWPQDGGDAVSGGRPGIGGDGGNLLSSISIESKYFTSVGGKSGKRAPDYKGGAAGLPNPAIHEYWVSGKLRQREIKEFRPGKDIDSPNAKREFGNDGRYKLLETTLGDHWLTNGYLANAIKYGKDLFLGNHVVQAHSYFLKLSQFCTDFTSKDVVRTNHCLSILSNLQNLNLGNNYFGNPLNNVPLISLETSTSIYKNELLNALEVIYFATLSKSKKLDTKDKVRILSNLQDSIYKELEKSNNQIPKLLWQIPDVNTLIDELRRDEKVFTAELLRVQAIIEQRARDNIIDRRRKAKLSKVLKSIATVASVIPVGKPALGASVEALTSFIDKGIDSNSLSDTIKNVSGLYKAVKKIEPTIAKSKEDWNNGFQKIRYSVLRDKLDEENKREERNKILKTYMKDITKFAYPIVENTLAEINSYRKISYPKDEVEKEILRLQQTDPMFNELTLLLKNILENRKSAAQKVRQINSRVSKIMSDINEGFNKIANISNEKFLLLERNTFLLGQDFESIIDDAKYRILFYNYNLYSSYQYRLFNNEEVGFYESLSSIIEIMENDILMNDGNESSDLMTYYRLNQDNLSKIFFKSINSLRSEELINDSTTIHLNEAQIRELNKGNEIFLNLYSIKDHFSGQENLKIRDVKIDTDFNDSYSDQSISKFLLTIEHGNDHIIEKNGITYKLGQERTYEWGVSVDTVSGHHSVLEPSKLNYSFLERLIGEDLSENEASQFYARPSLLGEYKVKFINKSYPKTQIDLKEVSIKIDYDYMKKN
>CP027772.1|AYF44252.1|1252871_1254923_+|dehydrogenase-E1-component
MANVLKPKKKYTWKNTDKATLKQWYELLTLGRMLDNRAPNYLKQAIGWSYHAPYAGHDGIQLAIGQTFELGKDHLYPYYRDMMTAFSAGLTTEEILLNGISKATDLASGGRHMSNHFAKPEWNIHNVSSCTGNHTLHAVGTARAFNRYKHDGVVYSSQGESSVSEGYCYEAINGASREKLPVVFVFQDNGYGISVPKCDQTANEYVADNFTGFLNLSIIHCDGKDIFDSMNAMATAREIAATQQEPVIVHARCVRIHSHSNSDKHELYRSEEERAEAAAQDPLVAFRKELIAKKIFTEAELDEMDAVAKAELLEAHKKAMKAPNPTPESIYDFVLPEPHVPTKYVDGTHNESGEEMKFIDAINGQLKKEFRNNPDTYIWGQDMANKDKGGIFNVSKGMQQEFGKERVFNGPIAEDYILGTANGFSRYNKDIRVVVEGAEFADYFWPAMEQYVECSHDYWRSNGAFAPNILIRLASGGYIGGGLYHSQNLEGNLAGIPGVRIVCPAFADDAAGLLRTAMRSEGPTLYLEPKALYNAKQAMTVVPEDFEVPFGKCRVRREGTDLSLITYGNTVHHCLEAAEKLSEQGYSVEVVDLRSIAPLDEEGILASIKKTNRALVVHEDKVFGGFGGEVAAMINDKGFELLDAPVQRVGSTFTPVGFNRILEKAILPNTDRVVETALKVLNY
>CP027772.1|AYF44262.1|1266908_1267472_-|scaffold-protein-Nfu/NifU-N-terminal-domain-protein
MSEVNIDIQPTPNPNALKFILNMDVKKNGSSTFRTPMECGEVNLGINLFTIRGIDQIHFFENVITITKFNYEDWEDIEEKVVETIKHDLPNHNPEFEEFDPEKERRENLPKELKDIEEVLDRTVRQYLQADGGDIQTVSYEDNILLVRYQGACGTCPSSTTGTLEAIKSTLRAEYNPEIDVYIAPDW
>CP027772.1|AYF44263.1|1267496_1268717_-|cysteine-desulfurase,-SufS-family
MALEIDNIRNQFPQLERKVHDRPLIYLDNAASTLKHISVIEALNNHYTNDVANIHRGVHYLSEHGTIQYEQTRDSIQDLLNAESRCEIIFTKGTTDSLNLVAASFVPHYVKKDDVILISTMEHHSNIVPWQIQAEKIEAKVKEIPITDDGDIDEEAYIELLKEGNVKLVSMAHISNSLGTINDIKKYISLAHDHGAYFCVDAAQSIAHEKVDVQDLNCDFLAFSSHKMFGPTGFGVLYGKEKLLESMPPYQGGGDMIDVVTIEKTTYNDLPHKFEAGTPHIAGGIGLKPAIDFLIDTDLNVIKKYEKELLDYATQKINEIEKVQVIGTAKQKASVLSFVVDGAHPHDLGTLLDRQGVAIRTGHHCTQPLMKRFNVSATARASFSLYNTKEEIDIFIKALNKALNFL
>CP027772.1|AYF44264.1|1268716_1269967_-|FeS-assembly-protein-SufD
MNIQELTDQYISDLGNYTKSQSNKEAFDNFKARGLPHTKMEDWLYTKLTDVLPENFKVTTQEAMLKNVKVAGKYQAVFLNGKYSKADSFLPEEITLEEVDLVAEDANCDEQDIFAMLNASAVEKVINITVPKNYVADDIISIVHLSDFEGEFSLPRIHVNADKFSKAEFVEIFTGSETKAYNISSVTNFKIADSANISHIKVQREGLNAFHAGSVNADIARDANFNSFTFTTGAKKSRNEIRVRMNDVGATCSVDGLYAINGEQHTDNFSLISHIKEKTESSQLFKGVLDDKARGVFTGKVLVCRDAQLVNSEQLNKNLLLSKKAHVDTRPQLEVYADDVKCAHGATVGQMSDEEAFYLQSRGLSKERVQKLLIHAFCSDAIMKIENQKLQDFLSEILFDSFEKEAFDHLDQSEAK
>CP027772.1|AYF44265.1|1269995_1270754_-|FeS-assembly-ATPase-SufC
MSLLTVKDVHARVEEKEILKGISIEVKPGEVHAIMGPNGSGKSTLSKVIAGHPSFEVTDGTIDYNINGKQTNLLELEPDERAKNGIFLGFQYPIEVPGVSNIDFLLESFNEVSKAQGAAEMKKEEFREFLRPKLELLEMREEFLDRPVNTGFSGGEKKKNEILQMAVLNPKLALLDETDSGLDIDALKIVAKGVNAIKSKYNGIVLVTHYQRLLDYIVPDYVHVLSGGKIIKSGGKELALELEEKGYDWLVK
>CP027772.1|AYF44266.1|1270778_1272212_-|FeS-assembly-protein-SufB
MSDTQLTKSEYKYGFFTDIETQEFPKGLNEDIVRMISAKKKEPEWLLEYRLKAFRLWKKMPMPNWAKLEIPEIDFEDLYYYAAPKSTESAPKSLDDLDPELLATFEKLGIPLSEQKRISGVAVDAVFDSVSVGTTYKEELEKVGVIFCSISEAVQDYPELVKKYLGTVVPPADNFYAALNAAVFSDGSFVYIPEGVTCPMDLSTYFRINAKETGQFERTLVVADKGSYVNYLEGCTAPQRDENQLHAAIVELIALDDAEIKYSTVQNWYAGDEQGKGGIYNFVTKRGNCLGKNSKISWTQVEAGSAITWKYPSCNLIGDNSQGAFYSVALTNNKMQADTGTKMIHIGKNTKSTIISKGISAEQSENNYRGLVKVMPSAVGAKNYSQCDSMLVGDKCSANTFPYIDVKNNSATVEHEASTSKISEDQLFYLQQRGMDMEKCISMVVNGFCSEVFKELPLEFSVEAVKLIEMKLENSIG
>CP027772.1|AYF44267.1|1272204_1272639_-|transcriptional-regulator
MYKVSKKTQYALTALKHMQEYGHTCEENLTSAREICDKYDMPFDPVSKVMQKLNNSGILSSIKGIKGGYFLSRPLSNLNLFEVSNIIEGKVKETACDALKGKCDKYLACELISPIEKINSHVNHHLISVSLEELLMNKTGVTHE
>CP027772.1|AYF44268.1|1272827_1273697_+|peptidase,-S54-family
MFMTNQDNDANQDNDLKRLHYIGSLRDEQIVSVIEKQLLNEGIHIRKEFENDFFHLFVVELKDLSQAREVYRVYIGGAKPIEVNKDWQYVQSLRMGPATIIILALCAIVFIFGWILKNENLYYLFAFSTSKLDPYHNLNAGEYWRIITPAFIHFGFMHVFFNLLWWKELGKLIEFTKGSFFLILLLLFTGIISNFLQAAMSPGFFGGLSGVVYGLLGFLWPYSRLNPNFKFKLPTTDIVLMTGWLFLGFFDVFNFKMANWAHGGGLVSGAILGIIFALIDRQKTQTDQV
>CP027772.1|AYF44269.1|1273659_1274919_-|pseudouridylate-synthase
MKKRLKIRLSENDIIFEDDNYIALNKKAGWPVHKTLDPMRDNCTDALKSYLKFREVGKSPYLVLVHRLDVETAGILLFAKSKEANKYLQDLFSSHQADKIDKRYLAICSGRPKTDEGTIENFIKAQREGRKEKMVVVSSGGKKAITNYWVKDFKNDYSLIEFQILTGRKHQIRLHAKSIGHPIFGDPIYSNSKEQDGQRLCAYKLSFFDKFSNQKIDLEIRPVFDLDNFRTKISDEFKYIIFNKPYNVLCQFGKDHKDQLSLIDYNLPKELYPVGRLDKDSEGLLILTNDGKYIDRMANAKSKVEKTYLVQVDGDITNEAIEELKKGVVIKDNYKTKPAKVKKIESNLVKVEQRDPPVRFRKNIPTSWIEVRISEGKNRQVRRMCAAVGFPTLRLIRTTIGNIKLGELKLGQFEFFDGQ
>CP027772.1|AYF44270.1|1274943_1275561_+|PF06080-family-protein
MTDLPISEACERNKEPIFNIIAPYLEKGSNQRIKKPKFFEVGSLTCQHSAYFAEKFSDVDFITSEIRANQETLKKCLSYYKQSFKNLNGPEVYEAGFNKIPKGYGAYYTANTMHIMSWKEAKTFIKDLAAAMDEDSVLFIYGPFKYHGKFTSESNEQFDKHLKERDPQSGIRNFEDICNNFLKKGVFLKEDIKMPANNQLLIFSK
>CP027772.1|AYF44271.1|1275566_1277333_-|arginine--tRNA-ligase
METNTILTKLTDILESSIKLAYPDAQFNRKDIYDSIGQAPNIEMAHYAFPTFRFAKALKQGPPQIAGAILDKIQEQETSKLIKDVKAQGPYLNFTLSADAYFENLISEVRSGNFFTKKLTSNEPKTMIEYSQPNTHKELHVGHMRNLCLGNALVRIKEYCDVDVHPVTYPGDSGTHVAKCLWYLKYHNQDTIPETNKGEWLGRIYTLANTKLEDELGSDKEDDNRAKLTEILKQLHSESGEFYDLWQETRQWSIDLMKKTYEWADVEFERWFFESEMDAPSLKICEDYYKDGTFIKDDGAIGMDLSDDKLGFCILIKSDGTGLYSTKDVALAIKKFEEFGVEKNIYIVDSRQAFHFKQVFKVLEKIGFEQAKDCYHLPYEMVELTDGAMSSRKGNIVPLTDLINKMEAKIKEDYLNKYQGEWDQAEIDETATKIANGAIKYGMIKIDNNRKIVFDMNEWLKLDGDTGPYLQYVYARINSLLVKQGFDKSKEFNLSNAASLENIKEYELMQKISLFNDVAIKAHEQNKTSLLTTYLFELGKLFNSFYAACPIASSEESLKQARLELAYATSEIIKHGLAILGIKVPGRM

Crispr_ID: CP027772_3

• CRISPR_ID: CP027772_3
• CRISPR_location: 1653949-1654055
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 1 spacers

Consensus repeat of CP027772_3

Consensus_repeat	Method
TCAAGCGACATTTTAGCGTAGCTAAAATTAGC	CRISPRCasFinder

spacers of CP027772_3

>3.1|1653981|43|CP027772|CRISPRCasFinder
GAAAAAATCTTTCTCAAAAAACTAATCACACAGGCCTCGCAAC

CRISPR arrays and Neighbor proteins around CP027772_3

CRISPR arrays

The CRISPR arrays of CP027772_3

>merge|CP027772|3|1653949-1654055|CRISPRCasFinder
TCAAGCGACATTTTAGCGTAGCTAAAATTAGCGAAAAAATCTTTCTCAAAAAACTAATCACACAGGCCTCGCAACTCAAGCGACATTTTAGCGCAGCTAAAATTAGC

>CP027772|3|2|1653949-1654055|CRISPRCasFinder
TCAAGCGACATTTTAGCGTAGCTAAAATTAGC	GAAAAAATCTTTCTCAAAAAACTAATCACACAGGCCTCGCAAC
TCAAGCGACATTTTAGCGCAGCTAAAATTAGC

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
CP027772.1|AYF44621.1|1663426_1664755_-|hypothetical-protein	unknown	unknown	unknown
CP027772.1|AYF44620.1|1663160_1663412_-|hypothetical-protein	unknown	unknown	unknown
CP027772.1|AYF44608.1|1647043_1647610_-|hypothetical-protein	unknown	unknown	gnl|CDD|224228
CP027772.1|AYF44604.1|1639321_1641256_-|ATP-dependent-metallopeptidase-HflB	unknown	unknown	gnl|CDD|273520
CP027772.1|AYF44617.1|1658441_1660559_-|putative-fatty-acid-oxidation-complex-subunit-alpha	unknown	unknown	gnl|CDD|236864
CP027772.1|AYF44611.1|1650213_1651152_-|FAD-dependent-oxidoreductase	unknown	unknown	unknown
CP027772.1|AYF44616.1|1657257_1658430_-|efflux-ABC-transporter,-permease-protein	unknown	unknown	gnl|CDD|226957
CP027772.1|AYF44605.1|1641400_1642549_-|tRNA(Ile)-lysidine-synthetase	unknown	unknown	gnl|CDD|238950
CP027772.1|AYF44613.1|1654216_1654837_-|hypothetical-protein	unknown	unknown	unknown
CP027772.1|AYF44622.1|1664759_1666064_-|hypothetical-protein	unknown	unknown	gnl|CDD|377350
CP027772.1|AYF44610.1|1649472_1650231_+|PF11251-family-protein	unknown	unknown	gnl|CDD|378614
CP027772.1|AYF44603.1|1638474_1639308_-|putative-dihydropteroate-synthase	unknown	unknown	gnl|CDD|273657
CP027772.1|AYF44609.1|1647788_1649423_+|NlpC-P60-family-hydrolase	unknown	unknown	gnl|CDD|368573
CP027772.1|AYF44606.1|1642959_1644156_+|hypothetical-protein	unknown	unknown	unknown
CP027772.1|AYF44607.1|1644142_1646998_+|putative-lipoprotein	unknown	unknown	unknown
CP027772.1|AYF44619.1|1661995_1663006_-|hypothetical-protein	unknown	unknown	gnl|CDD|377167
CP027772.1|AYF44614.1|1654947_1656018_-|beta-lactamase-family-protein	unknown	unknown	gnl|CDD|293841
CP027772.1|AYF44615.1|1656194_1657229_+|di-heme-cytochrome-C-peroxidase	unknown	unknown	gnl|CDD|224771
CP027772.1|AYF44618.1|1660572_1661862_-|acetyl-CoA-C-acyltransferase	unknown	unknown	gnl|CDD|238383
CP027772.1|AYF44612.1|1651272_1653636_+|Tex-like-protein-N-terminal-domain-protein	unknown	unknown	gnl|CDD|225094

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
CP027772.1|AYF44622.1|1664759_1666064_-|hypothetical-protein	gnl|CDD|377350	pfam04413, Glycos_transf_N, 3-Deoxy-D-manno-octulosonic-acid transferase (kdotransferase). Members of this family transfer activated sugars to a variety of substrates, including glycogen, fructose-6-phosphate and lipopolysaccharides. Members of the family transfer UDP, ADP, GDP or CMP linked sugars. The Glycos_transf_N region is flanked at the N-terminus by a signal peptide and at the C-terminus by Glycos_transf_1 (pfam00534). The eukaryotic glycogen synthases may be distant members of this bacterial family.	0.000891944
CP027772.1|AYF44608.1|1647043_1647610_-|hypothetical-protein	gnl|CDD|224228	COG1309, AcrR, Transcriptional regulator [Transcription].	0.000142877
CP027772.1|AYF44604.1|1639321_1641256_-|ATP-dependent-metallopeptidase-HflB	gnl|CDD|273520	TIGR01241, ATP-dependent_zinc_metalloprotease_FtsH, ATP-dependent metalloprotease FtsH. HflB(FtsH) is a pleiotropic protein required for correct cell division in bacteria. It has ATP-dependent zinc metalloprotease activity. It was formerly designated cell division protein FtsH. [Cellular processes, Cell division, Protein fate, Degradation of proteins, peptides, and glycopeptides].	0
CP027772.1|AYF44617.1|1658441_1660559_-|putative-fatty-acid-oxidation-complex-subunit-alpha	gnl|CDD|236864	PRK11154, fadJ, fatty acid oxidation complex subunit alpha FadJ.	0
CP027772.1|AYF44618.1|1660572_1661862_-|acetyl-CoA-C-acyltransferase	gnl|CDD|238383	cd00751, thiolase, Thiolase are ubiquitous enzymes that catalyze the reversible thiolytic cleavage of 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA, a 2-step reaction involving a covalent intermediate formed with a catalytic cysteine. They are found in prokaryotes and eukaryotes (cytosol, microbodies and mitochondria). There are 2 functional different classes: thiolase-I (3-ketoacyl-CoA thiolase) and thiolase-II (acetoacetyl-CoA thiolase). Thiolase-I can cleave longer fatty acid molecules and plays an important role in the beta-oxidative degradation of fatty acids. Thiolase-II has a high substrate specificity. Although it can cleave acetoacyl-CoA, its main function is the synthesis of acetoacyl-CoA from two molecules of acetyl-CoA, which gives it importance in several biosynthetic pathways.	8.70276e-166
CP027772.1|AYF44616.1|1657257_1658430_-|efflux-ABC-transporter,-permease-protein	gnl|CDD|226957	COG4591, LolE, ABC-type transport system, involved in lipoprotein release, permease component [Cell envelope biogenesis, outer membrane].	2.26268e-15
CP027772.1|AYF44605.1|1641400_1642549_-|tRNA(Ile)-lysidine-synthetase	gnl|CDD|238950	cd01992, PP-ATPase, N-terminal domain of predicted ATPase of the PP-loop faimly implicated in cell cycle control [Cell division and chromosome partitioning]. This is a subfamily of Adenine nucleotide alpha hydrolases superfamily.Adeninosine nucleotide alpha hydrolases superfamily includes N type ATP PPases and ATP sulphurylases. It forms a apha/beta/apha fold which binds to Adenosine group. This domain has a strongly conserved motif SGGXD at the N terminus.	8.19335e-52
CP027772.1|AYF44610.1|1649472_1650231_+|PF11251-family-protein	gnl|CDD|378614	pfam11251, DUF3050, Protein of unknown function (DUF3050). This bacterial family of proteins has no known function.	1.09748e-106
CP027772.1|AYF44603.1|1638474_1639308_-|putative-dihydropteroate-synthase	gnl|CDD|273657	TIGR01496, Dihydropteroate_synthase, dihydropteroate synthase. This model represents dihydropteroate synthase, the enzyme that catalyzes the second to last step in folic acid biosynthesis. The gene is usually designated folP (folic acid biosynthsis) or sul (sulfanilamide resistance). This model represents one branch of the family of pterin-binding enzymes (pfam00809) and of a cluster of dihydropteroate synthase and related enzymes (COG0294). Other members of pfam00809 and COG0294 are represented by model TIGR00284. [Biosynthesis of cofactors, prosthetic groups, and carriers, Folic acid].	1.53578e-40
CP027772.1|AYF44609.1|1647788_1649423_+|NlpC-P60-family-hydrolase	gnl|CDD|368573	pfam05708, Peptidase_C92, Permuted papain-like amidase enzyme, YaeF/YiiX, C92 family. Amidase_YiiX is a family of permuted papain-like amidases. It has amidase specificity for the amide bond between a lipid and an amino acid (or peptide). From the structure, a tetramer, each monomer is made up of a layered alpha-beta fold with a central, 6-stranded, antiparallel beta-sheet that is protected by helices on either side. The catalytic Cys154 in UniProtKB:Q74NK7, Structure 3kw0, is located on the N-terminus of helix alphaF. The two additional helices located above Cys154 contribute to the formation of the active site, where the lysine ligand is bound.	0.000445736
CP027772.1|AYF44619.1|1661995_1663006_-|hypothetical-protein	gnl|CDD|377167	pfam03938, OmpH, Outer membrane protein (OmpH-like). This family includes outer membrane proteins such as OmpH among others. Skp (OmpH) has been characterized as a molecular chaperone that interacts with unfolded proteins as they emerge in the periplasm from the Sec translocation machinery.	0.00110528
CP027772.1|AYF44614.1|1654947_1656018_-|beta-lactamase-family-protein	gnl|CDD|293841	cd16283, RomA-like_MBL-fold, Enterobacter cloacae RomA and related proteins; MBL-fold metallo hydrolase domain. Derepression of the romA-ramA locus results in a multidrug-resistance phenotype. Members of this subgroup belong to the MBL-fold metallo-hydrolase superfamily which is comprised mainly of hydrolytic enzymes which carry out a variety of biological functions. The class B metal beta-lactamases (MBLs) from which this fold was named are only a small fraction of the activities which are included in this superfamily. Activities carried out by superfamily members include class B beta-lactamases, hydroxyacylglutathione hydrolases, AHL (acyl homoserine lactone) lactonases, persulfide dioxygenases, flavodiiron proteins, cleavage and polyadenylation specificity factors such as the Int9 and Int11 subunits of Integrator, Sdsa1-like and AtsA-like arylsulfatases, 5'-exonucleases human SNM1A and yeast Pso2p, ribonuclease J and ribonuclease Z, cyclic nucleotide phosphodiesterases, insecticide hydrolases, and proteins required for natural transformation competence. Classical members of the superfamily are di-, or less commonly mono-, zinc-ion-dependent hydrolases, however the diversity of biological roles is reflected in variations in the active site metallo-chemistry.	6.07541e-71
CP027772.1|AYF44612.1|1651272_1653636_+|Tex-like-protein-N-terminal-domain-protein	gnl|CDD|225094	COG2183, Tex, Transcriptional accessory protein [Transcription].	0
CP027772.1|AYF44615.1|1656194_1657229_+|di-heme-cytochrome-C-peroxidase	gnl|CDD|224771	COG1858, MauG, Cytochrome c peroxidase [Inorganic ion transport and metabolism].	1.66699e-67

Protein sequences

>CP027772.1|AYF44612.1|1651272_1653636_+|Tex-like-protein-N-terminal-domain-protein
MSEETTKSTKNPMDVIDQNAVLFCVDNTDITAKQIMAVLAMLLVEDCTVPFITRYRKDKTGGLDEEQIRLIQEKYDEYIEREKRREFILEAITKQEMMTPEIEKKIKAATNINQLEDIYAPFKSKRKTKAQIAKEAGLGPFSILMKESKLPMPELAKEALKYINKEKKIATFEDVLKGACDIIIEEIAHDTELKEQLRNDYWAQAMIKSTPRKGYEEVKDWQKYKDYFEYEQKVSDLKDPKAAHRFLAIRRAQTEKILKVEIIFDEEYANNLILSKNFPDTTVTSYKALVDCASKAYKNYIHSSLDLEIKGELKKLSDESAIDVFGINLKNLLLQPYLGSKTVLALDPGVVTGCKTVVVDNTGKFVVDTVVYPHPPRNQVRESATILNAMIEQFNVEYIAIGNGTFGRETLQFVEDNITAVKDGKVKATMISEDGASIYSASPIAKKEFPDKDATVRGAISIGRRFQDPLAELVKIDPKSIGVGQYQHDVNQVRLKKSLNGVVESCVNFVGVDINTASAPLLSFISGIGPTVAGNIVKHRDKNGLFSDRSEILKVSRFSEKVFQQAAGFLRVYNGKNPLDATFIHPENYDMLEEWAQKNGYTLEQLTTDNAVANKLKSDKDLIAKLGEFTVNDIYKSLTAPSQDPRTEFKTTEFRKDVREIKDVKIGERYPGIVKNITQFGAFVDIGIKENGLVHVSQMADHFVENALDVLKVGQEVSAVVLDVDMDRKRIALSLKSDGAEEAKTYERPTGGPRKSNPRGGKPKMPKQNDAPLKNNAFAALKGFKVK
>CP027772.1|AYF44611.1|1650213_1651152_-|FAD-dependent-oxidoreductase
MTKDFDYIVIGDGVAARSILYFLTRKHPDSRICQLFDNDAFPPCSINTTSVVSMGLFAEGLSKLGDILFHAHEFIDTFFKQEGIQGVHPATQYYLPPDIKEDPLKFKQFEARYGTNFENFKGLQTVAKDAYLINPQVFLGWLKAQYEDKIISRVQRVTELPKTNAKLILATSAYTSLFYKNKNLPEGKPVKGTFFSWNNFKADKSFVLSRGHYNLVYRKEDELLLFGANSIEGLCQLHSPKEVYEKLTVFEGDLPELKQLGSPQIHTGIRHKGKRRTPFCGEVEEGVYALTGLYKNGWSAGISLAKELVDML
>CP027772.1|AYF44610.1|1649472_1650231_+|PF11251-family-protein
MKKENLTLIHAAHNKLCNHPLYSNIESIEDVKILMRWHVFAVWDFMSLLKGLQREVTCVETPWIPSKYDKECVRFINEIVLGEESDEDLNGGHIDHFTMYLNAMNEVGSETSDIKKFLETFSFDGIPMPVAKFSAFNLNLCTNTQNPHLMASAFFWGRENLIPDLFTPFVKVLEEKNIHAPYFKHYLERHIELDGDEHGELAGKLLAQLCDSEDKQRQATQIALDSLDLRYKLWDYVLKEILASRENLEHIN
>CP027772.1|AYF44609.1|1647788_1649423_+|NlpC-P60-family-hydrolase
MKKRLLTSGLCLLSLTIPIGCQQKSYRTPSSLKNSNFDSLIHEMHESVSKGVKSPKECAQKTQYYYGKLFEIESDDIDFSEFSKYQMQDFTMASFNIRLDLKEKLKALNSNDELTQEELACLKGVKTIFRALRYLEDYFIESYYASRQKGSKPIDFNTLDYSNNKIKDLPVHFMVNPDYSFEGVEDLKSGDIILSRGNAYSSAAIARIGDDDHQFSHLTLVYRDEKTNKLYTSEAHIEVGNVVAPFQVHLDQKNSRTVVFRHKDEKLAHLAGKIMHERFSKYSKEKKKNVPYDFAMDYHDDSEVFCSEVIYHGYKKASEQLSSKSMDIPEYKTTFNPALLSFLQDFGLKVTSENISSFKTFGPGEIQFDSRFDLVAEWRNPNKLKDTRYKDAVLTKIFEWMENKNYAFDPGLGTKLGNDFAWLARRSIVTRGLIKFVAGVDLEEKFPLNLGSKQIDLFVVLDEVGEQLYKKIEIAEKEKGKILSFKEMFEVLENYRVQDEKVYGQYLKDKKFNQKQMHGGSRRNGVRRRRKLTRPDFHHLFHSS
>CP027772.1|AYF44608.1|1647043_1647610_-|hypothetical-protein
MTDSTNLQTNDEGDNKPLRTVKPKKNEEVHFRILNAVTKLEVAKGHLNWKIAEVAKEADVTRSLIYYYLGKEKDVILKEAVKYMISTVFNLTQEHSVGIRERIKIVRDQIIQMPYLLALYMINKGAGNDLSDIIAEAEVELFEILSKKHPNLDHREHLRIYFMQLGICLYRDVDDDTLDYLFSQYDRK
>CP027772.1|AYF44607.1|1644142_1646998_+|putative-lipoprotein
MTFCKTFKNITLLSMSMLMLTTLVSCGSDTSNDEKQKLARQLKAQANESSVITVSRFSVEGVSEDKANVKSRIAYHKTFHVKACLSSKIGKSLQYRKFFVTGDNFQTIEREADESSCIRWDEPVEMDYSLPNTYLPMNRKIILSSSANISYDLKLAVNQYANSMIHGNNYHLSQGLNKDRLSNDEIINVKNIKITPKGYGLVDPRNDNLIIDKKVVVSACLNLKTNGSSLSNEFIKAKLVNLEKQENGEENAVTQMYDLTDGQLDESGCAEVKFYLEHERYTNTRRIPFNLEIEVQNKTLKNAKVTRGLCLYPWSNSGWIFAEVSRSGKCEEDNNNQRARLFVDQVSYSFLGHDNNNGFHLNNNLDLVVEKSFIVKMYPKVHYGNYAHHMNPIEPFYQGKLKLEVYFLAPLQGDIDLTNENKHKFKLISVASREVEVENNLLKARIDMPIKFVDMPYVHTRTFAAVKLSPILREGDDPRTAPRPAWAAGTFHASSKTFNSVLHTQLQVTDKFSEADYQNNKIKESMNEMIQLENYLESMSKNIQGVTKADIIKAKLAPRYTKSAEKLFTEELDKHDLFDKTINLTDRALNDKLIAENKLSQAEINGIYEDNYNANTLKKLCGAFFDQKKTGIFWKTYLDYNMKKCIERPWDFMMITPVSHVQEVISKPKVSHSNTIRISKSTGTGDYFTESNRVGWSHGYKTGVGAKLDFKLPFGDKSPVGGGVGVSASYDWSTSWSLDRSNGNNNRTDNSQGIDLYAESITLNFEAITKQCFMVEGTSKWKAVWVDNHLPSNYGLGGSLKYVAENNPMRYKVCKGVSGKERLEETWYFTGEGFQFNSILRDRLGMTENMYTMMFRGRKTLYKFMDFLSKASTSMFNIKERKNQSPDVYMLDVYKSYKEAFDSDHDLIDDQAIPGTVEKFDPIKNGGDHNFSEDSCELPKDNYNHASIDFC
>CP027772.1|AYF44606.1|1642959_1644156_+|hypothetical-protein
MQNKKLLNKHLILLGFVLSSPVLAQSGASKADVFNSGLESTLKNAGIDELSLTIGAKLGSLDSRTDKGTVYETNMNVELEAEVFEHVDFNFDGGFKYQTGNSSTQETGRIRTYKPKFSYKHGYFSYKMFNFAKISAGALKNSTNSTYISPFVNGGNSFLGVREMLLTKVGNFYFRLQATQAQPQNALLRETFNIDDNGDPQFFNESVDITYLTRGFKVYGAAGTYRYKNLSADIADNDYFWGNSVSKGATPDQSLYLYDFTGMYANAGIEFGIGNDRIRLKGAYVKNTESAVPDDKNTGFAYVAAYETKISDKKVTFEALGFENQADTAPAFYRGTQLANDYKGYQVGIKLENKKGFKTEINYMNRTVLGNADSVYLIDGYSDEQVYTISLRKEYDIL
>CP027772.1|AYF44605.1|1641400_1642549_-|tRNA(Ile)-lysidine-synthetase
MSLREIKQKQLPQKAKQMLAHFIRFNKGMNFDFSAPIVVACSGGLDSIALTHLLLMAGANVRLIHVDHGTREEIRAERHLVRNFAHKYQIPMKSLKVELSLNDGNFEAQARKLRYDILKNDLENDELLVTGHHLDDCFEWSLLQQFKSQGKDIDLGIPYRNGRVYRPLFAFSKKHILKYAKLAKLTWLEDYSNEDIRFERNHIRKIVKESISSRWPGYLKHYCERMNRMKKIKQGLEDKSYKIIKRKWGILIKHHGCVDENLKRTILSQASTTRGKTYKQFNELEGLINSPRLGPLDFSGGIRVYAFGPYLAVVKKGCKLEFLNNKTTQIPEGVPFLSNNTNAKHSLKKTPLENSTLRKIFSEIKQPVHYSWRKAGKVLVWL
>CP027772.1|AYF44604.1|1639321_1641256_-|ATP-dependent-metallopeptidase-HflB
MKGQNKTLLLWIILILMFAAIAKISSESRQTYKPVTYPDFVSAIKSKHVEKVVLKGKDKILGKFKDGYEQGAWFETTGTTGTETFKILESHGIYPEYEAEEKQNVFTTFLLHWGPMIFLFLIVIFFLRQLQAGGGKAMSFGKSKARLLSPHDKKVTFKDVSGVEEAKEELAEVVDFLKDPKKYTNLGGKIPKGVLLVGPPGTGKTLMARAVAGEAGVPFFSISGSDFVEMFVGVGASRVRDLFEQGKKNAPCIIFIDEIDAVGRHRGHGMGGGHDEREQTLNQLLVEMDGFESNEGVILIAATNRIDVLDKALLRPGRFDRRVTVGPPDVRGRLGILKVHTRKTPLDTNVDLEVIAKGTPGFTGADLANLVNEAALFAARYGKKVLQMIDFEQAKDKVLMGPERKSMVLSDFEKKVTAYHEAGHTLVGLNLPKADPIHKVSIMPRGGALGVTQTLPDEDMHNLTLEKSENYIAFLMGGRVAEEIVFNEISNGASNDIERATQLASSMVCSWGMSEKMGPRNFSQPGASPFGSTAPSEGVGYSEATAKDIDEEIHRFLDENYKLAKKILTDNRDALNRLAEGLVVWETLDINQINDLVAGKDIGVPVIDEESSKSSSTLSGEKEEASEEKKSDAVNKPIDDPKLA
>CP027772.1|AYF44603.1|1638474_1639308_-|putative-dihydropteroate-synthase
MTNILFLGVVNATPNSFSDGHRNLDNDFQIGQLEKFQQAKYGLDIGAESTAPFNEAISFKQEWQRLNDYISQHLDRLKAFKTISIDTYKVETMEEFMHSYGHHFSEVIWNDVSGAIDKEGLALFKKYPKMRYILCHNLAPKRNLANSHMDYLNENLDFNSIREFFNHKIAFLSEHNFDMDRLILDPCFGFSKTLNQNLELLENIHKIQDIHENWLIGISKKSFLQHLVRLEGNEDEMTDIHAASEKFHWKYLKRLKTRLNGVKELYFRVHEAGNITD
>CP027772.1|AYF44613.1|1654216_1654837_-|hypothetical-protein
MKKIFVILLGLLSINSLASEIKSLKYVSPWNYQMPAVEVLNNSTEHIEIIIKGALLQYPDFGFNFELSDRLGDVFELNNLNALIVKFPKSACKAEMAKDHLNIQLISCKNVLTFNQQDYSDQSQRMTQVKGAVVGFDGNIIEESDLVLNGFLSFHIKEVTERTVGRELNMIQAQVNGTIPFAGLNRRSLRIDLQKSQLALGPNNGL
>CP027772.1|AYF44614.1|1654947_1656018_-|beta-lactamase-family-protein
MTKVASVFMSSMGSHPDKEELKTFTKSSQFNKEKMRFVNRRPNITSEQPIKKDGFGFSNFIEFLGKGNGRYPDKPLPMLKPDMDKFLEKSDVVKSIWFGHSTVLFNIDSKIVLIDPIFSDSSSPVPIFVKRFGPTVLTLKELPKIDYILISHDHYDHLDMRTIKFFVDKDVKFITPLGVGSHLKRWGIDRKRVTELDWWQNVEFDGIKFTATPAQHFSGRDQAHENTTLWASWAVKTRNNNLYFSGDSGYDTHFKEIGQRLGPFDVAFMESGQYNPDWFAVHLLPDEWPKAYEDINANYYFPIHWGAFSLAFHTWDDPIIKLNEFSQQGKLKLMAPKMGEIVSFDHQEYITKKWWQ
>CP027772.1|AYF44615.1|1656194_1657229_+|di-heme-cytochrome-C-peroxidase
MKLTVYTISMLLLLSCTGKENNTDKKSLEERRELFGNGLNSIRYFGENRPKEEVIELGRMLFYDTRLSNDKTVSCSKCHLPQLSFSDALPKSIGAKYRLSHRNAQTLINTAAQISQHWNGNRKDVEEQAKKAFFFKGAYDLKDSSHLKKKLVDNNYNEAFKKAFNTELITSNENDILELSAKSIGAFERTLVGPAIFDDYLEGKDDVLNLEQKSGLKKFTQYGCIGCHNGNLVGGGMYQKFGVVEDPYKYTKSKNRDLGRYTHTMNEDDKEYFKVPPLRNVTKTSPYFHDGSVASLDEAIDIMGRVQLGVEIPKEDRKLIIEFLKTLETKEEHLKELTRIPDLP
>CP027772.1|AYF44616.1|1657257_1658430_-|efflux-ABC-transporter,-permease-protein
MFFSFFKFFLSYLFKAKTRQRLLIMALASLVISSFSLLTVQSVLKGLQSNRIERGKQTLGRYIIDLEKDFNFKKATSYLNDNNYKYTLEYEIEGLIRLEGYLAPVIFHGIIEDTSSILPKAIDHWPKRNEILMSPYLARKVYAGLDDRVQLISPAHTDTFFGELPRFKSLDVEAFTDSMDPDIDEFHAWGHAYSAFSIAKSRHYNKLRVFSNLTKIQEEQLKSKFAGNGARFHTWEELNQNLVFALALENNVVLFLFLATIVLVTFSIISGLSIFYARVRNDFASFWILGMSMGHIKKYGGLNIAIITASAIAVGNILSFIVLKLLVQFSPTIMPAMFVDRSLPVRFEASSFFYSFIVPVIITVVFTLLSNWRFFKDNGNFMTFVKKVGT
>CP027772.1|AYF44617.1|1658441_1660559_-|putative-fatty-acid-oxidation-complex-subunit-alpha
MTYKRLSFEIKDKKAFVGFGYNCDKAMTVLDEETLKELQDIVEDLHSKEKELDGAIFFSHKDRCFLAGADINLFDTMHTAADGQAGAEAGQTIFNRLEDLKMPTVACVSGVCLGGGLEFALSCKSIIVSDDKSTGLGLPEVKLGLIPGFGGTYRLPKKVGLPTALDMILTGKNLNAKKAKKVGIADEIYAKERLLDLAPKHFKKKADKRSMMDKVQDAATDNVFTKKIIFQKARESVLKKTKGFYQAPLKILDVMEGGVMKGRASYLTTEAQAFGELCIGEQSKNLRHIFFLMEGSKKMDDAKGVGRPLKRGACLGAGTMGGGIAWLMAKNDMFPIMKDLNQHGLELGLKQASSNFSGAVKRKRMSKDDFERKMRSISAQTDYRGFEHTDLVIEAVVENMDIKKKVFAETETKVRPDTILTSNTSSLSIQEMSKALEDSSRFAGLHFFNPVHMMPLVEIIKHDNVSDETVESLYNWVVKTKKTPVVVNDGPGFLVNRILMPYMNEAGFLLEEGVSIKDIDDACLNFGMPMGPFRLLDEVGIDVGDKVSKIIFDGLGARLASNGIGKQMLEKEFLGKKNSKGFYLYDEKGKVTGPNDEAWKMLPKASKKMSETEIQMRIFLPMINEAATTLEDKIVAKAKDVDLGLIFGIGFPPFRGGLLRYADTQGIARLKEEMLKFADSVDKDRYTPCELINKLASDNGKFYEL
>CP027772.1|AYF44618.1|1660572_1661862_-|acetyl-CoA-C-acyltransferase
MKPVYIVEAVRTPQAKSGTIIKDIQAPYLGAYLVRHIMDGTSIEDDAVDEVIFGNTGTPAKYPNIGRVIALEAGLHKKTSGYSVHRNCASGLEAASQGFLKVASGRSEIVVAGGVESMSNMPLIYGKQMTELFAKLMKAKTTGDKLKVLSSFKPAYLSPIVAIEQGLTDPFCGLNMGQTAELLARELGITREQQDEYANESHHKAIKAIEEGRFADEIVPLIYGAKLDKLLMNDHGPRKESSVEGLAKMKPYFDRKSGTVTVGNSCPITDGGSAILFCSEEAVKKYNLEPMARVVDYHFHGLEPERMGMGPLLAMDGVFRRTGLTLEQMDLVEINEAFAAQIIAVKKAFTDQKVADKFGVDKLWGEIPDEKLNVNGGAIALGHPVGSTGSRLVVTLAHELKKRKAGYGIASLCIGGGQGGAMIIENLQK
>CP027772.1|AYF44619.1|1661995_1663006_-|hypothetical-protein
MKTTKFNCAVMAALIFSSAASFAQEDPLLSETDQVVHTDEINLDGFYKKKKKKSAADKIQKLRRELEEKHEQMMRKNVEDMRLKEERRIAKKIQKALEGQIKAMDEINSIQSAPARSEVVAPVVPQGPQLDNKVSVQTGFKTITGDQKEWTAGLNFDAVVETKVSDRVDVGISVGYMGMTIGDQDLASNRGYSCSYSGCYSTGYYHSANDEISYNEFNFGGHAKFYFSRAAKIKPFLSLGTKVHFGNFKIENPRDSFNNTELSYRYVSGGATLGAEVNFAKEFGVQLALNYDKNIFRMGEEDEYAGSANDYVLNNQAKNLEGADSFTLGLGLSYLF
>CP027772.1|AYF44620.1|1663160_1663412_-|hypothetical-protein
MIESKKHNELNNDKGQTVVEYLLLITAIVSIMFAAFQTLDARFKADPGACDRGSINPLCFLDTIGLDSSTDVRADKFRYYNLR
>CP027772.1|AYF44621.1|1663426_1664755_-|hypothetical-protein
MNKNIDTLIIGNNITSFLTSSQILKSGHDAVYLKDQRIKSNDPYFKFYGPIDLAFIKTWISDIDEESSIFKKSNFELAPFCLLVNSKRYEFASPRPYLNYSQVTRQFPNLHAQLQQILASEQKEISDFEEDFLTAVSRLGESLCRYKTIHQVSVATFKSALPDYIYDCYSSFRREFKNNSELQKLMSSLRCIYTQSIELTFSDLELIYFFIVLLSPRYRFIWSLALEAQAKDAIEKLGGVTLEDTIEEFSFSNARPWATQLASYSGVVAPSKMAIFSSNVERLGIGHDLSSVYGCIKLNYKMSDNLLSGEYYHFDAHSFGSDVAFIRKKVSGDGVCEVKVYWRFEDCFKVDFVQDMFHKLLIENEIIDSQDVLLNCVAIDEVCLLRNHGLLNARENVKLKFRTGSRGKLESVKGVYYHGPLKKNNLGITQTLLEARDYKTFV
>CP027772.1|AYF44622.1|1664759_1666064_-|hypothetical-protein
MAYPFFLFFYLLRIGPFYRRVSFESKNYQDEGTRAFSRVGKKAHCLFHFSSEGEFEQIRPIIDELISHGKNVELLFTSPSVEHKVVEYYQAHRERVRYLRLPLITYFPLLKRQNIIAWSSASKMMMVRYDFFPELIYLGSKMSEFILFSATSKSSKARFNKDGKPIISWIKKFIYAQFSEVIAATIEDQKVFESAQLGTQIVAPIELRMLQINSRQKSFRKHKHYEFLSQILGLYPYQDRICLAQMWINEFTFFNDTNLLNDIADKKKFVFLAPHLLKEDKVEEMLSEIKKLSPKLKIHILDESKLTLESFQSFLKEITMNPGLILCTVKGILCEIYPYFHKVYVGGGHGKGVHSLLEPFVAGSRIFCGPNIHRSTEYDMIISSNFNIKIIEDCHEIYHIMSQVNEDSMKPEVISAMIEESELAKRKLINLLTK

Crispr_ID: CP027772_4

• CRISPR_ID: CP027772_4
• CRISPR_location: 2603895-2604047
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 1 spacers
• Cas_protein_info: DEDDh

Consensus repeat of CP027772_4

Consensus_repeat	Method
GCGCTAACGCTTGCTAAACAACCAAATGCTTACGCATTCGGTATCACGCGCT	CRISPRCasFinder

spacers of CP027772_4

>4.1|2603947|49|CP027772|CRISPRCasFinder
CCATTCTCTAATCTAATTTAAGATACTACATACTAATCGAAGATACGCA

CRISPR arrays and Neighbor proteins around CP027772_4

CRISPR arrays

The CRISPR arrays of CP027772_4

>merge|CP027772|4|2603895-2604047|CRISPRCasFinder
GCGCTAACGCTTGCTAAACAACCAAATGCTTACGCATTCGGTATCACGCGCTCCATTCTCTAATCTAATTTAAGATACTACATACTAATCGAAGATACGCAGCGCTAACGCTTGCTAAACAACCAAATGCTTACGCATTCGGTATCACGCGCT

>CP027772|4|3|2603895-2604047|CRISPRCasFinder
GCGCTAACGCTTGCTAAACAACCAAATGCTTACGCATTCGGTATCACGCGCT	CCATTCTCTAATCTAATTTAAGATACTACATACTAATCGAAGATACGCA
GCGCTAACGCTTGCTAAACAACCAAATGCTTACGCATTCGGTATCACGCGCT

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
CP027772.1|AYF45513.1|2600096_2601485_-|dihydrolipoyl-dehydrogenase	unknown	unknown	gnl|CDD|235798
CP027772.1|AYF45519.1|2605436_2607290_-|protein-glutamate-O-methyltransferase-CheR	unknown	unknown	gnl|CDD|214534
CP027772.1|AYF45507.1|2595825_2596653_+|nuclease,-EndA/NucM-family	unknown	unknown	gnl|CDD|225231
CP027772.1|AYF45508.1|2596708_2596840_-|hypothetical-protein	unknown	unknown	unknown
CP027772.1|AYF45509.1|2596961_2597684_+|methyltransferase-domain-protein	unknown	unknown	gnl|CDD|379312
CP027772.1|AYF45515.1|2602526_2602787_+|hypothetical-protein	unknown	unknown	unknown
CP027772.1|AYF45510.1|2597697_2597823_+|hypothetical-protein	unknown	unknown	unknown
CP027772.1|AYF45514.1|2601620_2602511_+|exonuclease	DEDDh	cd06127_DEDDh_CAS-I	gnl|CDD|176648
CP027772.1|AYF45523.1|2612298_2613675_-|peptidase,-M48-family	unknown	unknown	gnl|CDD|320683
CP027772.1|AYF45524.1|2613842_2614322_+|hypothetical-protein	unknown	unknown	unknown
CP027772.1|AYF45526.1|2615379_2619153_+|hypothetical-protein	unknown	unknown	gnl|CDD|379398
CP027772.1|AYF45520.1|2607476_2608109_-|NAD(P)H-binding-protein,-PF13460-family	unknown	unknown	gnl|CDD|187560
CP027772.1|AYF45516.1|2602783_2603713_+|KamA-family-protein	unknown	unknown	gnl|CDD|224426
CP027772.1|AYF45518.1|2604676_2605429_+|phosphoribosyl-transferase-domain-protein	unknown	unknown	gnl|CDD|224837
CP027772.1|AYF45517.1|2604166_2604667_+|CBS-domain-protein	unknown	unknown	gnl|CDD|341411
CP027772.1|AYF45512.1|2598419_2600072_-|2-oxoglutarate-dehydrogenase,-E2-component,-dihydrolipoamide-succinyltransferase	unknown	unknown	gnl|CDD|237000
CP027772.1|AYF45522.1|2609437_2612266_+|peptidase-MA-family-protein	unknown	unknown	unknown
CP027772.1|AYF45525.1|2614321_2615113_+|transglycosylase-SLT-domain-protein	unknown	unknown	unknown
CP027772.1|AYF45521.1|2608222_2609371_+|amino-acid-permease	unknown	unknown	gnl|CDD|129987
CP027772.1|AYF45511.1|2597776_2598385_+|hypothetical-protein	unknown	unknown	unknown

HMMScan for Signature genes

Protein	Cas_name	Cas_description	E-value	Identity	Coverage
CP027772.1|AYF45514.1|2601620_2602511_+|exonuclease	DEDDh	cd06127_DEDDh_CAS-I	1.7e-51	169.2	0.0

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
CP027772.1|AYF45513.1|2600096_2601485_-|dihydrolipoyl-dehydrogenase	gnl|CDD|235798	PRK06416, PRK06416, dihydrolipoamide dehydrogenase; Reviewed.	0
CP027772.1|AYF45507.1|2595825_2596653_+|nuclease,-EndA/NucM-family	gnl|CDD|225231	COG2356, EndA, Endonuclease I [DNA replication, recombination, and repair].	1.47928e-32
CP027772.1|AYF45514.1|2601620_2602511_+|exonuclease	gnl|CDD|176648	cd06127, DEDDh, DEDDh 3'-5' exonuclease domain family. DEDDh exonucleases, part of the DnaQ-like (or DEDD) exonuclease superfamily, catalyze the excision of nucleoside monophosphates at the DNA or RNA termini in the 3'-5' direction. These proteins contain four invariant acidic residues in three conserved sequence motifs termed ExoI, ExoII and ExoIII. DEDDh exonucleases are classified as such because of the presence of specific Hx(4)D conserved pattern at the ExoIII motif. The four conserved acidic residues are clustered around the active site and serve as ligands for the two metal ions required for catalysis. Most DEDDh exonucleases are the proofreading subunits (epsilon) or domains of bacterial DNA polymerase III, the main replicating enzyme in bacteria, which functions as the chromosomal replicase. Other members include other DNA and RNA exonucleases such as RNase T, Oligoribonuclease, and RNA exonuclease (REX), among others.	3.58982e-61
CP027772.1|AYF45518.1|2604676_2605429_+|phosphoribosyl-transferase-domain-protein	gnl|CDD|224837	COG1926, COG1926, Predicted phosphoribosyltransferases [General function prediction only].	5.41159e-70
CP027772.1|AYF45523.1|2612298_2613675_-|peptidase,-M48-family	gnl|CDD|320683	cd07324, M48C_Oma1-like, Oma1 peptidase-like, integral membrane metallopeptidase. This family contains peptidase M48 subfamily C (also known as Oma1 peptidase or mitochondrial metalloendopeptidase OMA1), including similar peptidases containing tetratricopeptide (TPR) repeats, as well as uncharacterized proteins such as E. coli bepA (formerly yfgC), ycaL and loiP (formerly yggG), considered to be putative metallopeptidases. Oma1 peptidase is part of the quality control system in the inner membrane of mitochondria, with its catalytic site facing the matrix space. It cleaves and thereby promotes the turnover of mistranslated or misfolded membrane proteins. Oma1 can cleave the misfolded multi-pass membrane protein Oxa1, thus exerting a function similar to the ATP-dependent m-AAA protease for quality control of inner membrane proteins. It has been proposed that in the absence of m-AAA protease, proteolysis of Oxa1 is mediated by Oma1 in an ATP-independent manner. Homologs of Oma1 are present in higher eukaryotes, eubacteria and archaebacteria, suggesting that Oma1 is the founding member of a conserved family of membrane-embedded metallopeptidases, all containing the zinc metalloprotease motif (HEXXH). M48 peptidases proteolytically remove the C-terminal three residues of farnesylated proteins.	6.19336e-48
CP027772.1|AYF45526.1|2615379_2619153_+|hypothetical-protein	gnl|CDD|379398	pfam13884, Peptidase_S74, Chaperone of endosialidase. This is the very C-terminal, chaperone, domain of the bacteriophage protein endosialidase. It releases itself, via the serine-lysine dyad at the N-terminus, from the remainder of the end-tail-spike. Cleavage occurs after the threonine which is the final residue of the End-tail-spike family, pfam12219. The endosialidase protein forms homotrimeric molecules in bacteriophages. The catalytic dyad allows this portion of the molecule to be cleaved from the more N-terminal region such that the latter can fold and presumably bind to DNA.	2.13292e-14
CP027772.1|AYF45520.1|2607476_2608109_-|NAD(P)H-binding-protein,-PF13460-family	gnl|CDD|187560	cd05250, CC3_like_SDR_a, CC3(TIP30)-like, atypical (a) SDRs. Atypical SDRs in this subgroup include CC3 (also known as TIP30) which is implicated in tumor suppression. Atypical SDRs are distinct from classical SDRs. Members of this subgroup have a glycine rich NAD(P)-binding motif that resembles the extended SDRs, and have an active site triad of the SDRs (YXXXK and upstream Ser), although the upstream Asn of the usual SDR active site is substituted with Asp. For CC3, the Tyr of the triad is displaced compared to the usual SDRs and the protein is monomeric, both these observations suggest that the usual SDR catalytic activity is not present. NADP appears to serve an important role as a ligand, and may be important in the interaction with other macromolecules. Atypical SDRs generally lack the catalytic residues characteristic of the SDRs, and their glycine-rich NAD(P)-binding motif is often different from the forms normally seen in classical or extended SDRs. Atypical SDRs include biliverdin IX beta reductase (BVR-B,aka flavin reductase), NMRa (a negative transcriptional regulator of various fungi), progesterone 5-beta-reductase like proteins, phenylcoumaran benzylic ether and pinoresinol-lariciresinol reductases, phenylpropene synthases, eugenol synthase, triphenylmethane reductase, isoflavone reductases, and others. SDRs are a functionally diverse family of oxidoreductases that have a single domain with a structurally conserved Rossmann fold, an NAD(P)(H)-binding region, and a structurally diverse C-terminal region. Sequence identity between different SDR enzymes is typically in the 15-30% range; they catalyze a wide range of activities including the metabolism of steroids, cofactors, carbohydrates, lipids, aromatic compounds, and amino acids, and act in redox sensing. Classical SDRs have an TGXXX[AG]XG cofactor binding motif and a YXXXK active site motif, with the Tyr residue of the active site motif serving as a critical catalytic residue (Tyr-151, human 15-hydroxyprostaglandin dehydrogenase numbering). In addition to the Tyr and Lys, there is often an upstream Ser and/or an Asn, contributing to the active site; while substrate binding is in the C-terminal region, which determines specificity. The standard reaction mechanism is a 4-pro-S hydride transfer and proton relay involving the conserved Tyr and Lys, a water molecule stabilized by Asn, and nicotinamide. In addition to the Rossmann fold core region typical of all SDRs, extended SDRs have a less conserved C-terminal extension of approximately 100 amino acids, and typically have a TGXXGXXG cofactor binding motif. Complex (multidomain) SDRs such as ketoreductase domains of fatty acid synthase have a GGXGXXG NAD(P)-binding motif and an altered active site motif (YXXXN). Fungal type ketoacyl reductases have a TGXXXGX(1-2)G NAD(P)-binding motif.	5.52153e-68
CP027772.1|AYF45516.1|2602783_2603713_+|KamA-family-protein	gnl|CDD|224426	COG1509, KamA, Lysine 2,3-aminomutase [Amino acid transport and metabolism].	3.32471e-83
CP027772.1|AYF45517.1|2604166_2604667_+|CBS-domain-protein	gnl|CDD|341411	cd17775, CBS_pair_bact_arch, Two tandem repeats of the cystathionine beta-synthase (CBS pair) domains present in bacteria and archaea. The CBS domain, named after human CBS, is a small domain originally identified in cystathionine beta-synthase and is subsequently found in a wide range of different proteins. CBS domains usually occur in tandem repeats. They associate to form a so-called Bateman domain or a CBS pair based on crystallographic studies in bacteria. The CBS pair was used as a basis for this cd hierarchy since the human CBS proteins can adopt the typical core structure and form an intramolecular CBS pair. The interface between the two CBS domains forms a cleft that is a potential ligand binding site. The CBS pair coexists with a variety of other functional domains and this has been used to help in its classification here. It has been proposed that the CBS domain may play a regulatory role, although its exact function is unknown. Mutations of conserved residues within this domain are associated with a variety of human hereditary diseases, including congenital myotonia, idiopathic generalized epilepsy, hypercalciuric nephrolithiasis, and classic Bartter syndrome (CLC chloride channel family members), Wolff-Parkinson-White syndrome (gamma 2 subunit of AMP-activated protein kinase), retinitis pigmentosa (IMP dehydrogenase-1), and homocystinuria (cystathionine beta-synthase).	8.74763e-34
CP027772.1|AYF45512.1|2598419_2600072_-|2-oxoglutarate-dehydrogenase,-E2-component,-dihydrolipoamide-succinyltransferase	gnl|CDD|237000	PRK11855, PRK11855, dihydrolipoamide acetyltransferase; Reviewed.	3.26301e-150
CP027772.1|AYF45509.1|2596961_2597684_+|methyltransferase-domain-protein	gnl|CDD|379312	pfam13649, Methyltransf_25, Methyltransferase domain. This family appears to be a methyltransferase domain.	1.09436e-14
CP027772.1|AYF45521.1|2608222_2609371_+|amino-acid-permease	gnl|CDD|129987	TIGR00909, putative_amino_acid_transporter, amino acid transporter. [Transport and binding proteins, Amino acids, peptides and amines].	1.45013e-36
CP027772.1|AYF45519.1|2605436_2607290_-|protein-glutamate-O-methyltransferase-CheR	gnl|CDD|214534	smart00138, MeTrc, Methyltransferase, chemotaxis proteins. Methylates methyl-accepting chemotaxis proteins to form gamma-glutamyl methyl ester residues.	1.74955e-56

Protein sequences

>CP027772.1|AYF45516.1|2602783_2603713_+|KamA-family-protein
MRKGLRSTNEVNSHFGLNLEPSSYEVFIPSHMNELIQKEGLEGIHAKQFLPSNLENSDDGLEDPIGDQQKYVAPQLIHRYDNRALFTPTTICPVICRYCFRKNELSSKHEIFKADFEKVISYLSVHTEINEIIFSGGDPFMLSASKIKFYLEEFSKLSHIQFIRFHTRTPISLPKYICDEFIEVIHKFNNRFNDINIVIHINHIQEITTDAMDCIKKLGARFGLLSQSVLLKDVNNNVKDLKDLFLKLHALNIRPYYLHHPDQAKGAMHFYLSIEEGREIYRKLRREIPGWLLPQYILDSPDGSGKKNL
>CP027772.1|AYF45515.1|2602526_2602787_+|hypothetical-protein
MLAGKPLSYKLQFVVIFIIHIALLYWIYWLLQNTGALSTMKVMAHFTGIAIYGALLIRGCAAWAKWHHLNDIKKLQDKQDKENNQK
>CP027772.1|AYF45514.1|2601620_2602511_+|exonuclease
METPTTSFIKGITINFTQSEIKNINKYFPDGLIALDLETSGLSPLVDEIIEIAAIKVVGNKIETFESLVKPKNPIPEFTTKIHGITDDMVKDSPSIEEVLPKFLEFSGNTPLVAHNAKFDIGFIVFAMHQQKIELHKAKVFCSCKLSRKYVKGSENHKLSSLCKHFEIPLENHHRALDDTIACLRVFAKTLDEVKSNRQLKEGEILNIRDFTRANMAEAPEIIQQLQKKIANQEVIDIKYKGGSYKGKFRPIKPVALLPMPAGDVLYAHCLLTDIYKSFAIKKIQDLRDHVDKDSK
>CP027772.1|AYF45513.1|2600096_2601485_-|dihydrolipoyl-dehydrogenase
MKKFDVIVIGSGPGGYVAAIRAAQLGKKTAIVESKELGGVCLNRGCIPTKAVLKAAHSVHELADMKDLGIDVELKGLDGGQAVKRAKGISNKISGGVAYLMKKNKIEVIEGYGKLRTNTQVEVTSKRGHTETIEGTNIILATGAHYRSFPGLEHDGKRLIGAWEAIKMEELPKSIGIIGAGAIGVEFAYFWNAFGVDVHIFELQKHLLPIEDEESSKAVEKAYKKYGIKMSLGVEKVSAKNNGNDITITEVKGGKTVEHKFDMGLIAVGMTGNIEGIGLETVGVKTDRGFVVVDNNYQTNIPNIFAIGDVAGPPLLAHAASHEGIIAAEKIAGEHPHPLDKMNVAGCTYCQPQVASVGYTERALKEQGIKYTAGKVPFSANGKAMASNETDGFVKTLMGEDGEMLGAHIVGTHATELIHEYVLFRQMEGVDEEMFATIHPHPTLGEFLAESVLNAKGRSINF
>CP027772.1|AYF45512.1|2598419_2600072_-|2-oxoglutarate-dehydrogenase,-E2-component,-dihydrolipoamide-succinyltransferase
MKNDIVMPQMGESITNGTITKWHKQVGDTIEIDEILLEISTDKVESEIPSPYSGKIVEILFEEGETIDVGVKIAAVDDDMNAEVSASGSSEAAAPAPAAAEPAQAAPAATGSSDTLEDVVMPQMGESITNGTITKWHKSVGDMIEIDEILLEISTDKVESEIPSPFEGRIEEILFEEGETIDVGVKIASVETDPSKPQGSAGSSAPAASAPAANQAQESAPAASSSQPSPTTKGGRRFYTPLVRNLAKKHGVDLNELAHIEGSGAGGRVNKADFMNFLENRGSAPKAAPAPAATKSAPAAPKAAPAPQASSVPSSTSGRVEVIPMDNMRKAIAKNMIASKMTSPHVNSMDEVDMTKLFKFREGFKHQFKKEEGFSLTYTHFILYALVQALKEFPMVNSSIVGDEIHMKKDINLGCAVAVPGNGLVVPVIKGADNLNIRGIARKLDELVQKARAKKLTLDDMSGGTYTFTNNGSFGILMATPVILQPQLGIFCVGTMKKRPIVTEDDAIAIRQMMYATHTYDHRLIDGEVGSKFLRHVINTLQTTDWEGLF
>CP027772.1|AYF45511.1|2597776_2598385_+|hypothetical-protein
MKTIVLISFALFLTFKTFASEINNIAISSHVNEFSIVDSKYAREIFLGLPVKNTYADLVHGLVFRYHKYFFCVREPSHKFHCHLYLNGLGQGKLSAYDTDTDFGKGAISEFLGTKRENGKAKISYDNNHLRINFSGKLAERFWLKLDLIKDSFHNINGKKFELRSGKHLNCYMHSGEKKSEYKCQFNVPIKENLKEIKKDLI
>CP027772.1|AYF45510.1|2597697_2597823_+|hypothetical-protein
MLAKSLICQAFVNLFYFDIFKLYILKYEDYRTHLFCPLSNI
>CP027772.1|AYF45509.1|2596961_2597684_+|methyltransferase-domain-protein
MFQFIRNLLNQKGFNAKAKRAFTNYYRFARESKSYLSYCNKMHGIECFMQNNLSKKQFEALTTHFKPNMKVLDIGCGTGDLTDYFAKNYKLKAKGMDLVESKFKYKEANFETTWLGENEYDLVISLDGFYMLNDLYRTLNKVMDSLKPKGKFVLTYTSERPFDKSRLGKALKKRKHDVKDFTQDDKVFNNKAKDLLQEMKSSFIEEGNSSLFHTKYNEIKKNVDSHQNGKMYRSLVTIYK
>CP027772.1|AYF45508.1|2596708_2596840_-|hypothetical-protein
MLKFLFFLSVLVTLANAAVATVFLEGLNERGQQEVQKVISIFR
>CP027772.1|AYF45507.1|2595825_2596653_+|nuclease,-EndA/NucM-family
MKFTIALATLLFALTTNATELNNYYPKETELHLSMKSNNSEALKEHLFDVLNSMHQETNGRDLLGCDSNKRGKCYRQRSLGYKGARKVLFGKLHLQEDNNGYFIEDVYCQKKLRRRQTRSMGPGQIPNSNVLNCEHTWPQSKFSRRFSKGLQKSDLHHLYPTDSRANSVRGNYEFDNVDGFPVDYENCTASQTQGNGAFEPPAKHKGNVARALFYFSVRYDIQISSHQEQVLREWDKQDPIDAEELKRNETIFRVQNNRNPFIDIPNLADRISDF
>CP027772.1|AYF45517.1|2604166_2604667_+|CBS-domain-protein
MSIEHIIQKNIVTINSDATIHDAAKQMYEKHVGAIIVVGHKEGKKKTPMGILTDRDIALSFGKKGKIEPYSKVSEIMTPNVVMCSPKDSIVDTIKKMRANGVRRIPVVNERDQLVGIISADDVIQRLGNELHDLSEIINLEIEKEAYISSPVEKDRQLNPQIKIVI
>CP027772.1|AYF45518.1|2604676_2605429_+|phosphoribosyl-transferase-domain-protein
MFKNRKEAGQLLGEEFKEKLLEFKNVAIIALPRGGVPIAFEIAKMHKLPLDIFCVKKVGAPNNEELALGAVTEEGIGYLNQRIINHLNIDDLQLHRLTKQKKEEVKKQAQEFRNSRPPIDLSGRSIFIVDDGIATGATMKAAIKFLKKDHNIKNIFAVAPVSSRQSYNEIIDLVDDILVLETPHNFLSVGEFYLEFPQVSNKEVCEYLETSNELRRQKEKKDSIRRFENEGGAIYESQDFTINKKHYHSK
>CP027772.1|AYF45519.1|2605436_2607290_-|protein-glutamate-O-methyltransferase-CheR
MSEAFKKGNDWDIISLANYVSEIVSKESGNILGPAQQSMVMSRLKKRMMTLGTHTPESYYEYLSKNYNKESSQLISMLTTHHTYFFREFTHFEYLLTNLDSIVANAKKRGDSTIRVLSAACSRGQEVYSLAMFFNHHLKNYPGMKYEIVGTDIDPESVKIAQNGVYRYREIKSIPTVYLTGNWMRGSGEISKFAKVNSHIKENCSFGVMNLLAPEKFIGGKKFDIVFCRNVFIYFDQTSIEKIVNNFSKFLHKNALFITGISESLKHTKLEFQTMAPSVYCLDPEEKVVEKPRPLSIVKDKTVPRTSPIAKTPIIPKPIRMLVVDDSSSVVKLLSKIFEKDDDFELVGTAMNGLEAQEFLKNNKVDAMTLDIHMPEMDGVEYLKKNFRSGHPNVVVVSSASREDTRYAQETLNNGACDFVEKPALNNLKERAEEIKNKIKMSFLNAAPVVTKVDRSFQKSFDIKNPETKARFFVGSFSDKKKIIASLDELRGNQPPVFLFFEGNGNFLEMISEEFKSVPNIQVYDGSTELENNSVYICDFKEHFEDINSKTKSFKKSLSVFGIVSKSVENAFFDLNETQILIEDTGDINDSIKEVVSDIFPWTSFAHLGTEYLADDE
>CP027772.1|AYF45520.1|2607476_2608109_-|NAD(P)H-binding-protein,-PF13460-family
MKVLILGATGLVGSHILSMALSDDRVEKVIAPVRKNIDAHPKLFAPIINFDEITKEEVCSWKCDVVICALGTTIKKAGSKAAFRRVDLEYPLTFGKLAKECAAKSYVLNSAMGANSDSLVFYNKVKGLVEEGLQSLNFDALTIVRPGLIGGQRDEFRFAEEIAKSFLKILGPLLPKRLRLNPAKRIAQTMLESALSDRLGVKIISSDKLV
>CP027772.1|AYF45521.1|2608222_2609371_+|amino-acid-permease
MYGIGTILGAGIYALIGKISATAGMMAPLSFIVSAILASMTAYSYTQLVKLFPKSAGEAEYIFQGFHSVLLSNIVGWLVAFTGVVSASTLIKGFVGYFQTFIHWTDFSVVFLIIILVTALAIKGIKESINIVIFFTIIEIVGLILICGSAWQDLSANEFMLPALLQNFSWSQFPMIISGAFLAFYAYIGFEDMVNLAEETNLPGPTISRSIYIAVLVSTILYVFVALVAIASIPLDELNQSKAPLKDMYVHHGGNALVISAIGIFAIFNGIIAQVIMASRILYGLRHPLKWMNFLSVINKKTQTPINATIVVAIIISLLVSLFPIKTLAASTSFIILIVFTFINLSLVSYRIKNREIQFRYLTIPIIASISNIVFLAFTALY
>CP027772.1|AYF45522.1|2609437_2612266_+|peptidase-MA-family-protein
MKLKVLLILLFLSLNTNAQYSFTESQSTPMNWKSIENDFAQVIYPENYKDRAIYMANLIEHYSKYVGLSYGVKKPEQVALIYRPEMANPNGFVTLAPRRTEWFDSAQFSSRLSSLEWHEMLSIHEYRHVIQQDIFNKKTVKFFDILMGDTGQQVLSFLAKKPWYMEGDAVYTETKYTPAGRGRSPRFMARLKALLLKGETPTYDQFVGGSYNDQLVNWYIYGYVLVSYGYKKYGDQFWNVVMNNVTARPYPNGIVGAIENSSGVDFYDFYYAAFDDLRKEWIGDKFNNLPVSEYNVKTNQYKNGANLFYVEYDLDNHYRLIKESKDEKEVLTDIAYSEDFTRLSYSKDYAIQSQFLPHWRYGFKSYSDLSLISLRDGDIKYITSGERLYNPHFNKDGSKITAINFDKNGKWIIQELNLEGAKLRSITNSKYDFLEAINLNNNEVIAIATRDGKGKTIVKANYSNNRVLELVPPTYNNLYALNYDGNNSVLFEAQYKGKIEVFKLNTTTKSLEKCTDSNIASYSPTFSNGNFYFTEQTINGKRIRSSSLNSCRSLGINSLVDRSQYFAKNDPSDSYNNFELIDIKDRKEIQTKNSNNYTEEDYSGFDWRSATPHSWSFFVGNGYGLSLYADNYLRDFSWNVNLGYEASENSPYSSAIVDFKRYWPILSATVDLRERMVSQLASDTDDYGWDEVAYGIKVELPFVQKHNLYNLNLSLSYQAEQVMTDEYIQYDTKVSGEADFIRQSTTLSLSLYKDFTTRSIISPWSFSLLGMYQDASTDDETGLEDDYRWFGSFSITTPGLFTNNGIKISVVGEEYGDGDNYRFQPSSNEFMGYAYSRGYDYFASKKYLKSSANYLFPLFYPDYNIAALIYFKRIYMNLYYDITKAKLSDTRKSIQLSSYGAELVFNTNILRALPVDFGVRYIHKYETQEDEYEGYLGTSLAF
>CP027772.1|AYF45523.1|2612298_2613675_-|peptidase,-M48-family
MKNIGMLAVLALLVSCNAEKITTVKVLHSLDSDALSGEIKVNSQKLNFNGSSGETFEIKGTYPRNLSMDLDYTHAEAFYANSVNKLPATEINMKFEVRGYNSEKGTVDLRREGSVKLQGEKFKLTNCSNTVDFKVDGSELTIDMSKFKSLASCKFVGKYKNHNRIGEKGRLHNGFGRNFFKFDSDKDMGLEASYAIRSQFSGYVYKDGEMAEYLQKMLTKIAKASDMPDLEPKVYFINAPIENAFALPGGYVFVFRGLIEALETEAELAGVLGHEWAHVTERHGTESVSRNLKNIATKYVGAGLVWAQFNIFGAAAAYYIYDKLEEIPLMGFSRLQELEADKIGVQYAHEAGYSPYGLSKFFAAMGDRQESWLELLVSTHPRPSTRVEKIEELVFNYIPYSRDAVVTSEDYLAAKETMNEIEPMPYYIAMRVMAQMGGLVEESIKSSVRKKFKLEKED
>CP027772.1|AYF45524.1|2613842_2614322_+|hypothetical-protein
MRNRQESTKLNILKLLIVICITLPSFGDEIKSFLDVAKKHVGEYEVIKSNSRFCDSGTLAFLNSSKPKDGLRIGQTIFFGPFSKEKQIEKTSDGCKSTSRYKFENNKVTQFSEIIECPKKFKKLEAKSTQVLSFSDEQVSYHSVESKIKCIFKRKKGGN
>CP027772.1|AYF45525.1|2614321_2615113_+|transglycosylase-SLT-domain-protein
MKTLVILFLSFNLYANETTPYSTAQFLEEINVVIDQVVAPPEEPCIDCKVEDNLISEADPLPTTFRRGSLYFNKMCENFVKEDGSLGVWGDMIVNYIDNTEGASDIYLYDEVRGMTASPYTCPNWYNLNNQQRKRFWVWMFASIAQVESSCDPTKVNTGPVPDATDRPTGLFQLNQVKRNRMWRGENCKFETGYTATIKPENQIKCSMDIMMEILKGRRGIYRGSGRIFPTNSYWEKLRPNHSQTGGPIGELVRNYPPCKATP
>CP027772.1|AYF45526.1|2615379_2619153_+|hypothetical-protein
MQTDGTPITGTVDLRFEVFNSTGPTLLETININSVTLSNGIYSTELVIPNYQTHVEDLTGSETLLIRVTDVTNGLTFDYQKILSVPMAFYANKAVTADSIANAAITPDSLDFVGGCLDNQVLIKSGNQFDCINQTAALTVDSTLVNNAGVLGQATVGTAGTYTSVTVDQYGRVTSGSNPEVGSADITDGSIVDADISSTAAISWSKINAPTIDKTYVGLGNVLNVAQIPASDLDNSGTLDSSTQVPSELAVKGYVDTYADAKVIDDMSGTQTTIAPSVNSVKNYVTTEIGNVNQTQWTTTGSDIYYNAGLVGIGTSTPSAELTIEDGSLLIDHTTNDKRFRISQNGAATYLQFGDRLNNGGQLRFSTPYTGGTTFMTLDSTTQNVGIGTTSPTEKLEVNGNIKVSSTSDVCIDGGACLSGAAGGDITDVVAGTGLTGGATTGSATLNVDVGTGANQIVQLDGTSKLPAVDGSQLTNLPNPTSSTTLSDFATGADSPITNTDTVETAFEKTQGQINGNNTDIATNTTNISSNTSALTNKVDKTTTVNGQALSGNITLDSDDVSEGTTNLYFNDSRAQSAAVINSTAGSETVQAPSVDAMKNYVLAQTGAINESQWITSGSDIYFSSGNVGIGTSTPSTTLTVNGNSTVWGKHEVDYNGINDALHVHQEGTGNLLSLSTGAGWDQKLIVDSSGFMGIGTSSPSNELEVNGIITSNNFGLSLPSGNDFNTKSLPAGLYRSNTTSNGSPTPGESGALLNMQSGVESGAVAQLHIKANNTENPRAFIRHSDNTGNFKDWQEIITANALGNVGIGTDLPTTKLTVDGNINVTTGNDICVDGSGCLSTVVAANGEPNTASSAGGASLVLTKTGTDLPFKGLTATTDIALTENANDIQIGVNTSNGAGELLRLDGSGRVPASNVMSTPLTGYTVGTNSVLAATDTILDAYGKLQAQINANYTAITGLDTDDVAEGTNLYFTTARAQTAAVVNSTAGTETTQAPSVSAVKNYVTTQMGTINQSQWTTNGSNIYFNTGNVGIGTTTPQNLLHVSGALNSHIYLEDRSGGVDNKIWSFNNNDGVLYLGQRNDDASYKNTHMTIDTLGNIGVGTITPSEKLHVAGNVIAASYLYTSDERFKENVETIIDPLHKVRSLRGVTFDWKTDEFPDRNFPQEKTVGFIAQEVEKVQPELVKTSKDGYKSVQYGNITALLVEAVKSISEQLDKLFSNDEEVKREIASLKEENEQLKAEMQEMRAAIKELQKANKE

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity
CP027772_1	1.8|531028|24|CP027772|CRT	531028-531051	24	MN693683	Marine virus AFVG_250M978, complete genome	13978-14001	2	0.917
CP027772_1	1.8|531028|24|CP027772|CRT	531028-531051	24	MN698239	Pelagibacter phage HTVC023P, complete genome	16359-16382	3	0.875
CP027772_1	1.6|530923|24|CP027772|CRT	530923-530946	24	MN694208	Marine virus AFVG_250M460, complete genome	13983-14006	4	0.833
CP027772_1	1.6|530923|24|CP027772|CRT	530923-530946	24	MN694657	Marine virus AFVG_250M407, complete genome	20415-20438	4	0.833
CP027772_1	1.6|530923|24|CP027772|CRT	530923-530946	24	MN693833	Marine virus AFVG_250M459, complete genome	20544-20567	4	0.833
CP027772_1	1.1|530605|33|CP027772|CRT	530605-530637	33	MN693047	Marine virus AFVG_25M111, complete genome	34315-34347	6	0.818
CP027772_1	1.1|530605|33|CP027772|CRT	530605-530637	33	MN693574	Marine virus AFVG_25M141, complete genome	11009-11041	7	0.788
CP027772_1	1.1|530605|33|CP027772|CRT	530605-530637	33	AP014024	Uncultured Mediterranean phage uvMED isolate uvMED-GF-C114-MedDCM-OCT-S41-C54, *** SEQUENCING IN PROGRESS ***	9501-9533	8	0.758
CP027772_1	1.1|530605|33|CP027772|CRT	530605-530637	33	MN693506	Marine virus AFVG_25M112, complete genome	34075-34107	8	0.758
CP027772_1	1.1|530605|33|CP027772|CRT	530605-530637	33	NC_012439	Persephonella marina EX-H1 plasmid, complete sequence	17722-17754	10	0.697
CP027772_1	1.1|530605|33|CP027772|CRT	530605-530637	33	MN698248	Pelagibacter phage HTVC202P, complete genome	4547-4579	10	0.697
CP027772_1	1.1|530605|33|CP027772|CRT	530605-530637	33	AP014446	Uncultured Mediterranean phage uvMED isolate uvMED-CGF-C43-MedDCM-OCT-S31-C62, *** SEQUENCING IN PROGRESS ***, 3 ordered pieces	27590-27622	10	0.697
CP027772_1	1.1|530605|33|CP027772|CRT	530605-530637	33	AP013774	Uncultured Mediterranean phage uvMED isolate uvMED-CGF-C43-MedDCM-OCT-S30-C105, *** SEQUENCING IN PROGRESS ***	7594-7626	10	0.697
CP027772_1	1.1|530605|33|CP027772|CRT	530605-530637	33	AP013559	Uncultured Mediterranean phage uvMED isolate uvMED-CGR-C43-MedDCM-OCT-S32-C95, *** SEQUENCING IN PROGRESS ***, 2 ordered pieces	4401-4433	10	0.697
CP027772_1	1.1|530605|33|CP027772|CRT	530605-530637	33	NZ_AP014641	Leptolyngbya boryana IAM M-101 plasmid pLBY	32687-32719	11	0.667

1. spacer 1.8|531028|24|CP027772|CRT matches to MN693683 (Marine virus AFVG_250M978, complete genome) position: , mismatch: 2, identity: 0.917

ctttgtagcttttttggctacctt	CRISPR spacer
ccttgtagcttttttcgctacctt	Protospacer
*.************* ********

2. spacer 1.8|531028|24|CP027772|CRT matches to MN698239 (Pelagibacter phage HTVC023P, complete genome) position: , mismatch: 3, identity: 0.875

ctttgtagcttttttggctacctt	CRISPR spacer
ctttgtagctttttttgctatctc	Protospacer
*************** ****.**.

3. spacer 1.6|530923|24|CP027772|CRT matches to MN694208 (Marine virus AFVG_250M460, complete genome) position: , mismatch: 4, identity: 0.833

ggcaacttttttctttgctgtttt	CRISPR spacer
cataacttttttctttgctctttt	Protospacer
 ..**************** ****

4. spacer 1.6|530923|24|CP027772|CRT matches to MN694657 (Marine virus AFVG_250M407, complete genome) position: , mismatch: 4, identity: 0.833

ggcaacttttttctttgctgtttt	CRISPR spacer
cataacttttttctttgctctttt	Protospacer
 ..**************** ****

5. spacer 1.6|530923|24|CP027772|CRT matches to MN693833 (Marine virus AFVG_250M459, complete genome) position: , mismatch: 4, identity: 0.833

ggcaacttttttctttgctgtttt	CRISPR spacer
cataacttttttctttgctctttt	Protospacer
 ..**************** ****

6. spacer 1.1|530605|33|CP027772|CRT matches to MN693047 (Marine virus AFVG_25M111, complete genome) position: , mismatch: 6, identity: 0.818

cgttgctttttttgctacttttttctttgttgc	CRISPR spacer
agttttcttttttgcaacttttttcttagttgc	Protospacer
 *** ..******** *********** *****

7. spacer 1.1|530605|33|CP027772|CRT matches to MN693574 (Marine virus AFVG_25M141, complete genome) position: , mismatch: 7, identity: 0.788

cgttgctt-tttttgctacttttttctttgttgc	CRISPR spacer
-attacctgtttttgctgcttttttctttgcttc	Protospacer
 .**.*.* ********.************.* *

8. spacer 1.1|530605|33|CP027772|CRT matches to AP014024 (Uncultured Mediterranean phage uvMED isolate uvMED-GF-C114-MedDCM-OCT-S41-C54, *** SEQUENCING IN PROGRESS ***) position: , mismatch: 8, identity: 0.758

cgttgctttttttgctacttttttctttgttgc	CRISPR spacer
tacagctttattttctacttttttctttggagc	Protospacer
... ***** *** ***************  **

9. spacer 1.1|530605|33|CP027772|CRT matches to MN693506 (Marine virus AFVG_25M112, complete genome) position: , mismatch: 8, identity: 0.758

cgttgctttttttgctacttttttctttgttgc	CRISPR spacer
tagttttctttttgcaacttttttcttagttgc	Protospacer
.. * .*.******* *********** *****

10. spacer 1.1|530605|33|CP027772|CRT matches to NC_012439 (Persephonella marina EX-H1 plasmid, complete sequence) position: , mismatch: 10, identity: 0.697

cgttgctttttttgctacttttttctttgttgc	CRISPR spacer
aactgctttttttactgcttttttcttcgccat	Protospacer
 ..**********.**.**********.*....

11. spacer 1.1|530605|33|CP027772|CRT matches to MN698248 (Pelagibacter phage HTVC202P, complete genome) position: , mismatch: 10, identity: 0.697

cgttgctttttttgctacttttttctttgttgc	CRISPR spacer
ctcggcttttgttgctacttttttatttcccat	Protospacer
* . ****** ************* *** ....

12. spacer 1.1|530605|33|CP027772|CRT matches to AP014446 (Uncultured Mediterranean phage uvMED isolate uvMED-CGF-C43-MedDCM-OCT-S31-C62, *** SEQUENCING IN PROGRESS ***, 3 ordered pieces) position: , mismatch: 10, identity: 0.697

cgttgctttttttgctacttttttctttgttgc	CRISPR spacer
ctcggcttttgttgctacttttttatttcccat	Protospacer
* . ****** ************* *** ....

13. spacer 1.1|530605|33|CP027772|CRT matches to AP013774 (Uncultured Mediterranean phage uvMED isolate uvMED-CGF-C43-MedDCM-OCT-S30-C105, *** SEQUENCING IN PROGRESS ***) position: , mismatch: 10, identity: 0.697

cgttgctttttttgctacttttttctttgttgc	CRISPR spacer
ctcggcttttgttgctacttttttatttcccat	Protospacer
* . ****** ************* *** ....

14. spacer 1.1|530605|33|CP027772|CRT matches to AP013559 (Uncultured Mediterranean phage uvMED isolate uvMED-CGR-C43-MedDCM-OCT-S32-C95, *** SEQUENCING IN PROGRESS ***, 2 ordered pieces) position: , mismatch: 10, identity: 0.697

cgttgctttttttgctacttttttctttgttgc	CRISPR spacer
ctcggcttttgttgctacttttttatttcccat	Protospacer
* . ****** ************* *** ....

15. spacer 1.1|530605|33|CP027772|CRT matches to NZ_AP014641 (Leptolyngbya boryana IAM M-101 plasmid pLBY) position: , mismatch: 11, identity: 0.667

cgttgctttttttgctacttttttctttgttgc	CRISPR spacer
atttgctttatttgctacttttctctaataaag	Protospacer
  ******* ************.***     .