CRISPRimmunity

Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Self targeting spacer number	Target MGE spacer number	Prophage number	Anti-CRISPR protein number
CP023408	Streptomyces fungicidicus strain TXX3120 plasmid p1, complete sequence	0 crisprs	csa3,WYL,c2c9_V-U4	0	0	0	0
CP023407	Streptomyces fungicidicus strain TXX3120 chromosome, complete genome	5 crisprs	WYL,csa3,casR,DEDDh,cas4,Cas9_archaeal,cas3,RT,DinG	5	3	0	0

1. CP023407

CRISPR-Cas detection and classification

Crispr_ID: CP023407_2

• CRISPR_ID: CP023407_2
• CRISPR_location: 1733262-1733380
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 1 spacers

Consensus repeat of CP023407_2

Consensus_repeat	Method
CTGAACACCACGCAGGAGGCCGGTCGATGAGCG	CRISPRCasFinder

spacers of CP023407_2

>2.1|1733295|53|CP023407|CRISPRCasFinder
TCAGCACCGAGTCCGGCCGGGGGTCCGGGGGTCGCCCCCCGGGACAGCACAGC

CRISPR arrays and Neighbor proteins around CP023407_2

CRISPR arrays

The CRISPR arrays of CP023407_2

>merge|CP023407|2|1733262-1733380|CRISPRCasFinder
CTGAACACCACGCAGGAGGCCGGTCGATGAGCGTCAGCACCGAGTCCGGCCGGGGGTCCGGGGGTCGCCCCCCGGGACAGCACAGCCTGAACACCACGCAGGAGGCCGGGCGATGAGCG

>CP023407|2|1|1733262-1733380|CRISPRCasFinder
CTGAACACCACGCAGGAGGCCGGTCGATGAGCG	TCAGCACCGAGTCCGGCCGGGGGTCCGGGGGTCGCCCCCCGGGACAGCACAGC
CTGAACACCACGCAGGAGGCCGGGCGATGAGCG

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
CP023407.1|AYL35340.1|1736172_1736457_-|GlsB/YeaQ/YmgE-family-stress-response-membrane-protein	unknown	unknown	gnl|CDD|182475
CP023407.1|AYL35343.1|1738707_1740336_-|cation-acetate-symporter	unknown	unknown	gnl|CDD|271374
CP023407.1|AYL35344.1|1740332_1740689_-|DUF485-domain-containing-protein	unknown	unknown	gnl|CDD|377315
CP023407.1|AYL35345.1|1740960_1742490_+|peptidase-S8	unknown	unknown	gnl|CDD|173810
CP023407.1|AYL39400.1|1736620_1737100_+|hypothetical-protein	unknown	unknown	gnl|CDD|378630
CP023407.1|AYL35346.1|1742542_1744075_+|peptidase-S8	unknown	unknown	gnl|CDD|173799
CP023407.1|AYL35333.1|1728440_1729139_+|GntR-family-transcriptional-regulator	unknown	unknown	gnl|CDD|225097
CP023407.1|AYL35331.1|1726531_1726648_-|hypothetical-protein	unknown	unknown	unknown
CP023407.1|AYL35329.1|1725552_1726071_-|phosphohistidine-phosphatase	unknown	unknown	gnl|CDD|224973
CP023407.1|AYL35328.1|1724249_1725458_+|phosphoserine-phosphatase-SerB	unknown	unknown	gnl|CDD|319803
CP023407.1|AYL35336.1|1731262_1731547_-|hypothetical-protein	unknown	unknown	gnl|CDD|226603
CP023407.1|AYL35347.1|1744160_1745534_+|hypothetical-protein	unknown	unknown	gnl|CDD|367110
CP023407.1|AYL35341.1|1737324_1737549_+|hypothetical-protein	unknown	unknown	unknown
CP023407.1|AYL35334.1|1729222_1729990_-|enoyl-[acyl-carrier-protein]-reductase-FabI	unknown	unknown	gnl|CDD|236124
CP023407.1|AYL35330.1|1726136_1726343_-|hypothetical-protein	unknown	unknown	unknown
CP023407.1|AYL35332.1|1727041_1728370_-|MFS-transporter	unknown	unknown	gnl|CDD|129974
CP023407.1|AYL35335.1|1729996_1730701_-|3-oxoacyl-[acyl-carrier-protein]-reductase	unknown	unknown	gnl|CDD|187594
CP023407.1|AYL35342.1|1737561_1738551_-|GTP-3',8-cyclase-MoaA	unknown	unknown	gnl|CDD|234672
CP023407.1|AYL39399.1|1730870_1731257_-|PIN-domain-containing-protein	unknown	unknown	gnl|CDD|350222
CP023407.1|AYL35339.1|1734833_1736102_+|tyrosine--tRNA-ligase	unknown	unknown	gnl|CDD|235645

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
CP023407.1|AYL35335.1|1729996_1730701_-|3-oxoacyl-[acyl-carrier-protein]-reductase	gnl|CDD|187594	cd05333, BKR_SDR_c, beta-Keto acyl carrier protein reductase (BKR), involved in Type II FAS, classical (c) SDRs. This subgroup includes the Escherichai coli K12 BKR, FabG. BKR catalyzes the NADPH-dependent reduction of ACP in the first reductive step of de novo fatty acid synthesis (FAS). FAS consists of four elongation steps, which are repeated to extend the fatty acid chain through the addition of two-carbo units from malonyl acyl-carrier protein (ACP): condensation, reduction, dehydration, and a final reduction. Type II FAS, typical of plants and many bacteria, maintains these activities on discrete polypeptides, while type I FAS utilizes one or two multifunctional polypeptides. BKR resembles enoyl reductase, which catalyzes the second reduction step in FAS. SDRs are a functionally diverse family of oxidoreductases that have a single domain with structurally conserved Rossmann fold (alpha/beta folding pattern with a central beta-sheet) NAD(P)(H) binding region and a structurally diverse C-terminal region. Classical SDRs are typically about 250 residues long, while extended SDRS are approximately 350 residues. Sequence identity between different SDR enzymes are typically in the 15-30% range, but the enzymes share the Rossmann fold NAD binding motif and characteristic NAD-binding and catalytic sequence patterns. These enzymes have a 3-glycine N-terminal NAD(P)(H) binding pattern: TGxxxGxG in classical SDRs. Extended SDRs have additional elements in the C-terminal region, and typically have a TGXXGXXG cofactor binding motif. Complex (multidomain) SDRs such as ketoreductase domains of fatty acid synthase have a GGXGXXG NAD(P) binding motif and an altered active site motif (YXXXN). Fungal type type ketoacyl reductases have a TGXXXGX(1-2)G NAD(P)-binding motif. Some atypical SDRs have lost catalytic activity and/or have an unusual NAD(P) binding motif and missing or unusual active site residues. Reactions catalyzed within the SDR family include isomerization, decarboxylation, epimerization, C=N bond reduction, dehydratase activity, dehalogenation, Enoyl-CoA reduction, and carbonyl-alcohol oxidoreduction. A critical catalytic Tyr residue (Tyr-151, human 15-hydroxyprostaglandin dehydrogenase (15-PGDH) numbering), is often found in a conserved YXXXK pattern. In addition to the Tyr and Lys, there is often an upstream Ser (Ser-138, 15-PGDH numbering) and/or an Asn (Asn-107, 15-PGDH numbering) or additional Ser, contributing to the active site. Substrates for these enzymes include sugars, steroids, alcohols, and aromatic compounds. The standard reaction mechanism is a proton relay involving the conserved Tyr-151 and Lys-155, and well as Asn-111 (or Ser). Some SDR family members, including 17 beta-hydroxysteroid dehydrogenase contain an additional helix-turn-helix motif that is not generally found among SDRs.	8.91789e-122
CP023407.1|AYL35343.1|1738707_1740336_-|cation-acetate-symporter	gnl|CDD|271374	cd11480, SLC5sbd_u4, Uncharacterized bacterial solute carrier 5 subfamily; putative solute-binding domain. SLC5 (also called the sodium/glucose cotransporter family or solute sodium symporter family) is a family of proteins that co-transports Na+ with sugars, amino acids, inorganic ions or vitamins. Prokaryotic members of this family include Vibrio parahaemolyticus glucose/galactose (vSGLT), and Escherichia coli proline (PutP) and pantothenate (PutF) cotransporters. One member of the SLC5 family, human SGLT3, has been characterized as a glucose sensor and not a transporter. This subfamily belongs to the solute carrier 5 (SLC5) transporter family.	0
CP023407.1|AYL35345.1|1740960_1742490_+|peptidase-S8	gnl|CDD|173810	cd07484, Peptidases_S8_Thermitase_like, Peptidase S8 family domain in Thermitase-like proteins. Thermitase is a non-specific, trypsin-related serine protease with a very high specific activity. It contains a subtilisin like domain. The tertiary structure of thermitase is similar to that of subtilisin BPN'. It contains a Asp/His/Ser catalytic triad. Members of the peptidases S8 (subtilisin and kexin) and S53 (sedolisin) clan include endopeptidases and exopeptidases. The S8 family has an Asp/His/Ser catalytic triad similar to that found in trypsin-like proteases, but do not share their three-dimensional structure and are not homologous to trypsin. Serine acts as a nucleophile, aspartate as an electrophile, and histidine as a base. The S53 family contains a catalytic triad Glu/Asp/Ser with an additional acidic residue Asp in the oxyanion hole, similar to that of subtilisin. The serine residue here is the nucleophilic equivalent of the serine residue in the S8 family, while glutamic acid has the same role here as the histidine base. However, the aspartic acid residue that acts as an electrophile is quite different. In S53 the it follows glutamic acid, while in S8 it precedes histidine. The stability of these enzymes may be enhanced by calcium, some members have been shown to bind up to 4 ions via binding sites with different affinity. There is a great diversity in the characteristics of their members: some contain disulfide bonds, some are intracellular while others are extracellular, some function at extreme temperatures, and others at high or low pH values.	3.16625e-55
CP023407.1|AYL39400.1|1736620_1737100_+|hypothetical-protein	gnl|CDD|378630	pfam11298, DUF3099, Protein of unknown function (DUF3099). Some members in this family of proteins are annotated as membrane proteins however this cannot be confirmed. Currently no function is known.	1.24873e-20
CP023407.1|AYL35333.1|1728440_1729139_+|GntR-family-transcriptional-regulator	gnl|CDD|225097	COG2186, FadR, Transcriptional regulators [Transcription].	4.9504e-51
CP023407.1|AYL35340.1|1736172_1736457_-|GlsB/YeaQ/YmgE-family-stress-response-membrane-protein	gnl|CDD|182475	PRK10457, PRK10457, hypothetical protein; Provisional.	1.16833e-08
CP023407.1|AYL35328.1|1724249_1725458_+|phosphoserine-phosphatase-SerB	gnl|CDD|319803	cd07500, HAD_PSP, phosphoserine phosphatase (PSP), similar to Methanococcus Jannaschii PSP and Saccharomyces cerevisiae SER2p. This family includes Methanococcus jannaschii PSP, and Saccharomyces cerevisiae phosphoserine phosphatase SER2p, EC 3.1.3.3, which participates in a pathway whereby serine and glycine are synthesized from the glycolytic intermediate 3-phosphoglycerate; phosphoserine phosphatase catalyzes the hydrolysis of phospho-L-serine to L-serine and inorganic phosphate, the third reaction in this pathway. This family belongs to the haloacid dehalogenase-like (HAD) hydrolases, a large superfamily of diverse enzymes that catalyze carbon or phosphoryl group transfer reactions on a range of substrates, using an active site aspartate in nucleophilic catalysis. Members of this superfamily include 2-L-haloalkanoic acid dehalogenase, azetidine hydrolase, phosphonoacetaldehyde hydrolase, phosphoserine phosphatase, phosphomannomutase, P-type ATPases and many others. HAD hydrolases are found in all three kingdoms of life, and most genomes are predicted to contain multiple HAD-like proteins. Members possess a highly conserved alpha/beta core domain, and many also possess a small cap domain, the fold and function of which is variable. HAD hydrolases are sometimes referred to as belonging to the DDDD superfamily of phosphohydrolases.	3.95697e-94
CP023407.1|AYL35336.1|1731262_1731547_-|hypothetical-protein	gnl|CDD|226603	COG4118, Phd, Antitoxin of toxin-antitoxin stability system [Cell division and chromosome partitioning].	9.20567e-05
CP023407.1|AYL35347.1|1744160_1745534_+|hypothetical-protein	gnl|CDD|367110	pfam02515, CoA_transf_3, CoA-transferase family III. CoA-transferases are found in organisms from all lines of descent. Most of these enzymes belong to two well-known enzyme families, but recent work on unusual biochemical pathways of anaerobic bacteria has revealed the existence of a third family of CoA-transferases. The members of this enzyme family differ in sequence and reaction mechanism from CoA-transferases of the other families. Currently known enzymes of the new family are a formyl-CoA: oxalate CoA-transferase, a succinyl-CoA: (R)-benzylsuccinate CoA-transferase, an (E)-cinnamoyl-CoA: (R)-phenyllactate CoA-transferase, and a butyrobetainyl-CoA: (R)-carnitine CoA-transferase. In addition, a large number of proteins of unknown or differently annotated function from Bacteria, Archaea and Eukarya apparently belong to this enzyme family. Properties and reaction mechanisms of the CoA-transferases of family III are described and compared to those of the previously known CoA-transferases.	1.28926e-40
CP023407.1|AYL35344.1|1740332_1740689_-|DUF485-domain-containing-protein	gnl|CDD|377315	pfam04341, DUF485, Protein of unknown function, DUF485. This family includes several putative integral membrane proteins.	5.01421e-34
CP023407.1|AYL35334.1|1729222_1729990_-|enoyl-[acyl-carrier-protein]-reductase-FabI	gnl|CDD|236124	PRK07889, PRK07889, enoyl-[acyl-carrier-protein] reductase FabI.	1.95644e-174
CP023407.1|AYL35346.1|1742542_1744075_+|peptidase-S8	gnl|CDD|173799	cd07473, Peptidases_S8_Subtilisin_like, Peptidase S8 family domain in Subtilisin-like proteins. This family is a member of the Peptidases S8 or Subtilases serine endo- and exo-peptidase clan. They have an Asp/His/Ser catalytic triad similar to that found in trypsin-like proteases, but do not share their three-dimensional structure and are not homologous to trypsin. The stability of subtilases may be enhanced by calcium, some members have been shown to bind up to 4 ions via binding sites with different affinity. Some members of this clan contain disulfide bonds. These enzymes can be intra- and extracellular, some function at extreme temperatures and pH values.	1.35508e-56
CP023407.1|AYL35332.1|1727041_1728370_-|MFS-transporter	gnl|CDD|129974	TIGR00896, Inner_membrane_transport_protein_YeaN, cyanate transporter. This family of proteins is involved in active transport of cyanate. The cyanate transporter in E.Coli is used to transport cyanate into the cell so it can be metabolized into ammonia and bicarbonate. This process is used to overcome the toxicity of environmental cyanate. [Transport and binding proteins, Other].	8.39095e-128
CP023407.1|AYL35329.1|1725552_1726071_-|phosphohistidine-phosphatase	gnl|CDD|224973	COG2062, SixA, Phosphohistidine phosphatase SixA [Signal transduction mechanisms].	1.50692e-40
CP023407.1|AYL35342.1|1737561_1738551_-|GTP-3',8-cyclase-MoaA	gnl|CDD|234672	PRK00164, moaA, GTP 3',8-cyclase MoaA.	0
CP023407.1|AYL39399.1|1730870_1731257_-|PIN-domain-containing-protein	gnl|CDD|350222	cd09874, PIN_MT3492-like, VapC-like PIN domain of the hypothetical protein MT3492 of Mycobacterium tuberculosis CDC1551 and other uncharacterized, annotated PilT protein domain proteins. Virulence associated protein C (VapC)-like PIN (PilT N terminus) domain of Mycobacterium tuberculosis CDC1551, hypothetical protein MT3492, and similar bacterial and archaeal proteins are included in this subfamily. They are PIN domain homologs of the Mycobacterium tuberculosis VapC and Neisseria gonorrhoeae FitB toxins of the prokaryotic toxin/antitoxin operons, VapBC and FitAB, respectively, which are believed to be involved in growth inhibition by regulating translation. These toxins are nearly always co-expressed with an antitoxin, a cognate protein inhibitor, forming an inert protein complex. Disassociation of the protein complex activates the ribonuclease activity of the toxin by an, as yet undefined mechanism. The PIN domain belongs to a large nuclease superfamily. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.	3.19228e-14
CP023407.1|AYL35339.1|1734833_1736102_+|tyrosine--tRNA-ligase	gnl|CDD|235645	PRK05912, PRK05912, tyrosyl-tRNA synthetase; Validated.	0

Protein sequences

>CP023407.1|AYL35336.1|1731262_1731547_-|hypothetical-protein
MSTISVREFSYNPSAVFARVEKGEAIEVTRHGNVIAILSPAGSPMERYAHLVAQGKIKLKPVTAAERHSMPHYEVPDDVDPVALLMAEREDDDR
>CP023407.1|AYL39399.1|1730870_1731257_-|PIN-domain-containing-protein
MDTTALVTWMTRRSHWDALDAFLQARATEPLATSTLGFVETARTLDLMGHFPKAMEDLDARITEILLTREVRDSAAPLVGRLRTLDAIHVASALSLRDDLTALVTYDRRMLETARAEGLSAHAPGMTD
>CP023407.1|AYL35335.1|1729996_1730701_-|3-oxoacyl-[acyl-carrier-protein]-reductase
MSRSVLVTGGNRGIGLAIARAFADAGDKVAVTYRSGEPPAGFLAVKCDITDPEQVEQAYKEIEEAHGTVEVLVANAGVTKDQLLMRMTEEDFSTVLDTNLTGTFRVVKRANRGMLRAKKGRVVLISSVVGLLGSAGQANYAASKAGLVGFARSLARELGSRNITFNVVAPGFVDTDMTKALTDDQRAKIVSQVPLGRYAKPDEIAAAVRFLASDDASYITGAVIPVDGGLGMGH
>CP023407.1|AYL35334.1|1729222_1729990_-|enoyl-[acyl-carrier-protein]-reductase-FabI
MSGILEGKRVLITGVLMESSIAFHTARLAQEQGAEIILTAFPRPTLTERIAKRLPKPTKVIELDVTNDEHLGRLADVVGEELGGLDGVVHSIGFAPQDALGGNFLNTPFESVATAMHVSAFSLKSLTMACLPLMQNGGSVVGLTFDAQYAWPQYDWMGPAKAALEATNRYMARDLGKQNIRCNLISAGPLGSMAAKSIPGFGELASVWDSRAPLEWDLKDPEPAGRGVVALLSDWFPKTTGEIVHVDGGLHAIGA
>CP023407.1|AYL35333.1|1728440_1729139_+|GntR-family-transcriptional-regulator
MPLSHPRRSALSEQVIAALRTQITSGEWAVGTRIPTEPELVEQLGVARNTVREAVRALAHNGLLDIRQGSGTYVVATSELAGVMHRRFAGADPRHIAELRSTLESSAARLAAERRTEKDLKQIDTLLLRREEAWESGEEEAFVAADATFHLAVVAASHNDVLTAMYADLSEVLRDWLRGDVNGDMSPRSHMDHGRLVDAIRAGDAATAAEEAAAYPFVCRPGRIGPPSAARG
>CP023407.1|AYL35332.1|1727041_1728370_-|MFS-transporter
MMVRMAREETRTLTSTTVRTPAPHAARTAPGTPAARPWRTRLLVIGIVLAALNLRPAITSLGALLEEVRDGLGMSGSVAGLLTSVPPLCFAVFGVTAPRLARRFGAGAVVCAGMVAIGAGLLIRPYVGGTAAFLAASALALMGIAVSNVLMPVIVKRWFPDRVGSMTGLYSMALALGTATAAAATVPLTGALGGNWQSGLAVWAALAVAAVLPWVPFVRGRDRQRDGAHGARTEPVTPADAPPLRITRSRTAWALAVFFGLQATAAYITMGWMAQIFRDAGVPAGTAGLLLAVTMVMGVPLAFVIPRLATRLPHQGPIALALGVSGLVGYAGLYLAPAAGAWVWAVLLGIANCAFPLALTMVGMRARTGAGVAQLSAFAQSTGYLISIPGPLLVGVLYQHSGGWGLPIALMAALMIPQMAVGVLAGRPRTVEDESQPPTPSH
>CP023407.1|AYL35331.1|1726531_1726648_-|hypothetical-protein
MPVLDPNPQNGQKKMLLVFGTFFAIFIVIGVIATILAP
>CP023407.1|AYL35330.1|1726136_1726343_-|hypothetical-protein
MSAHTHTRPHPAPRGGVDTRLPWWALALPTLAFVALLTLVLNPSDAHAATGEPALTHLVERAQQLIAR
>CP023407.1|AYL35329.1|1725552_1726071_-|phosphohistidine-phosphatase
MSVAEPRRIVLFRHAKADWPPVSDHERPLAERGRMDAAVAGRKLADTGIAFDLALCSTSVRTRETWKLAVHEFPERPKTVYEERIYEASPGELIALLNETPDDAQNVLLVGHNPGVQALADVLAGAAEGDARERMSRRGYPAAAFAVLSFTGSWKSLEPGVGTLADYWAPTE
>CP023407.1|AYL35328.1|1724249_1725458_+|phosphoserine-phosphatase-SerB
MSASQTSDVPTLLVKIFGKDRPGITAGLFDTLAAYSVDVVDIEQVVTRGRIVLCALVTEPPSGLEGDLRATVHSWAESMKMQAEIISGLGDNRPRGLGRSLVTVLGHPLTSEATATIAARITKAGGNIDRIFRLAKYPVTAVEFAVSGVETEPLRTALVTDAARFGVDIAVVGAGLYRRAQRLVVMDVDSTLIQDEVIELFAAHAGCEGEVAEVTASAMRGELDFEQSLHARVALLKGLDASVVDKVRSEVRLTPGARTLIRTLKRLGYQVGVVSGGFTQVTDDLKERLGLDFAQANTLEIVDGKLTGRVTGEIVDRAGKARLLRRFAAEAGVPLSQTVAIGDGANDLDMLNAAGLGVAFNAKPVVREAAHTAVNVPFLDTVLYLLGVTREEVEAADTLDVD
>CP023407.1|AYL35339.1|1734833_1736102_+|tyrosine--tRNA-ligase
MTDIVDELKWRGLFAQSTDEDALRKVLADGPVTFYCGFDPTAPSLHVGHLVQVLTLRRLQQAGHRPLALVGGATGQIGDPRPTAERTLNAPETVAGWVERLRGQIEPFLTFEGENAATMVNNLDWTQNLSAIEFLRDIGKHFRVNKMLTKDSVARRLESSEGISYTEFSYQLLQAMDFLQLYRRYGCVLQQGGSDQWGNLTAGLDLIHRLEPEASVHAYATPLMTKADGTKFGKTEGGAVWLDPEMTTPYAFYQFWLNVDDRDISGYMRILSFRSRAELEELEKQTAERPQARAAQRALAEELTTLVHGAGQTAAVIAASKALFGQGELGELDDRTLAAALSEVPHVRVSELGPVVDLFAEVGLVPSKSAARRTVKEGGAYVNNVKVTAEDAVPATEDLLHGRWLVLRRGKKNLAAVEVTAG
>CP023407.1|AYL35340.1|1736172_1736457_-|GlsB/YeaQ/YmgE-family-stress-response-membrane-protein
MGWLWAIIVGFVLGLIAKAVIPGKQHSPLWLTTICGILGAIVGNSMARGFGIEETPGFDWGRHIFQLGAAIVIVFIVDMLYTSMKRKRHEQQRV
>CP023407.1|AYL39400.1|1736620_1737100_+|hypothetical-protein
MFGPGGTERVTGGQVRASPDAGGRWNVMRKQHGGGSVQVFRITGARTGLQDDVRARQRRYVISMAVRTISVILAATLWNVERHVAIVALVLGAVLPYVAVVIANAGRENAPSLPSTFVTTPMKPMIAPARTNDGSAESDAEDVASQAAPGARTERPERS
>CP023407.1|AYL35341.1|1737324_1737549_+|hypothetical-protein
MVPDPETPICSAKGCRAAAVWVLAWNNPKLHTPERRKTWLACEEHREHLSQFLGVRGFLKDVVPFDDWKAPEGA
>CP023407.1|AYL35342.1|1737561_1738551_-|GTP-3',8-cyclase-MoaA
MLIDTYGRVATDLRVSLTDRCNLRCTYCMPEEGLQWLAKPDLLTDDEIVRLVGIAVTSFGIEEVRFTGGEPLLRPGLVGIVERVAALDPRPQMSLTTNGIGLRRTAAALKAAGLDRVNVSLDTLRPDVFKTLTRRNRHQDVLDGLSAAREAGLTPVKVNSVLMPGLNDDEAPDLLAWAVEQDYELRFIEQMPLDAQHGWKRDGMITAGDILTSLRTRFELTPEGDEARGSAPAERWLVDGGPHRVGVIASVTRPFCAACDRTRLTADGQVRTCLFATEETDLRAALRSGAPDEEIARLWRLAMWGKKAGAGLDDPTFVQPDRPMSAIGG
>CP023407.1|AYL35343.1|1738707_1740336_-|cation-acetate-symporter
MSPAITHVTLAANEASEHRTLIITLFALFVVATLGITVWAGRQTKDAADFYAGGRQFSAFQNGLAVSGDYMSAASFLGIAGAIALFGYDGFLYSIGFLVAWLVALLLVAEPLRNSGRYTMGDVLAYRMRQRPVRTAAGTSTIVVSIFYLLAQMAGAGVLVSLLLGITSDAGKILIVALVGVLMIVYVSIGGMKGTTWVQMVKAVLLIGGTLLITFLVLLKFNFNISDLLGSAAENSGKGEAFLEPGLQYGADGTTKLDFISLGIALVLGTAGLPHILIRFYTVPNAKAARKSVNWAIGIIGGFYLMTIALGFGAAALISQDEIIASNPSGNTAAPLLALHLGGVESAWGAILLATISAVAFATILAVVAGLTLASSSSFAHDIYANVIRKGKATEKEEMRAARWATVLIGIVSIGLGALARDLNVAGLVALAFAVAASANLPTILYSLFWKRFTTQGALWSIYGGLVVAVGLVLFSPVVSGDPKAMFPDVDFAWFPLKNPGIISIPFGFLMGWLGTVLSKEEPDAKKYAELEVRSLTGTGAH
>CP023407.1|AYL35344.1|1740332_1740689_-|DUF485-domain-containing-protein
MAAEAPPPSKEQHKLPTPEEFTEVQESAEFGELRRSHRSFAFPLTIAFVVWYLLYVLLSNYAGGFMGTKLFGNINVALVFGLLQFVTTFLIAWWYSRHAAAQLDPKAEAIKTRMEGGA
>CP023407.1|AYL35345.1|1740960_1742490_+|peptidase-S8
MAHLRSRRRLVLAVPVVLSLTASLGFIPAAASAAPRTAAAEQAADAPALAYVVNTKADHRTIASVKRAIAAADGKVVATYDRIGVIVVHSANPDFGKEIRAVRGVQSAGATRTSPLTPAGTTDAGAADFVTAAEAARTRAASALTPGSEPLEADQWDLRAIGADKAAKINPGSGKVTVAVIDTGVDDTHPDLAPNFSASQSANCVGGKADTSEGAWRPYTADDYHGTHVAGEIAAARNGVGVAGVAPGVKVAGIKVSDPDSGLFYPENVVCAFVFAADHGVEITNNSYYVDPWLYNCMDDPDQKAIVDAVNRAQLYAQKKGTLHLASAGNSNHDLASDAIDDASSPNDSTPVERTIDPSECYDIPTQLPGVVTVSATGVQNLKSYYSTYGKGVVDVAAPGGDRRYQLPDTPSKDGRILSTMPNGQYAWLQGTSMASPHAAGVAALLKSKHPRATPAQLQALLKAQANNPGCPESYDQDGDGVQDATCEGGSRVNGFYGFGIVDALRAVK
>CP023407.1|AYL35346.1|1742542_1744075_+|peptidase-S8
MTASLPRHRRALVIPAGMAMATALAFLPNTAATAAPADAAPAAVSADASSLSYVVNVKGGHATSAYVKKAIAKAGGTVVTSYDKIGVIVVHSSNADFAKTIRTVRGVQSAGATRNAPLPSAVTADMGAPKALSAAEIARAESAAAADQDPLQGLQWDLPAIKADKAHEKSLGSSKVTVAVIDTGVDDTHPDIAPNFDRAASVNCVTGKPDTSDGAWRPGAQESPHGTHVAGEIAAAKNGVGMTGVAPGVKVSGIKVSNPDGYFYTEAVVCGFMWAAEHGVDVTNNSYYTDPWYFNCKNDPDQTALLEAVTRATRYAERKGAVNVAAAGNENYDLAADEITDPVSPNDGTPSDRVIDPTECLDIPTQLPGVVTVAATGAKGLKSSFSNYGRGVIDIAAPGGDSTRYQTPEPPATSGLILGTLPGGSWGYMAGTSMASPHVAGVAALIKSTHPKASPALVKAMLYAQADATACTDPYDIDGDGKVDAVCEGPKNRNGFYGWGTADALDAVTR
>CP023407.1|AYL35347.1|1744160_1745534_+|hypothetical-protein
MTDITSVWSALGGDPALLARVSRVERPGTLPARLPVRESARSCVGACALAAAELGARRTGGGVPAVTVDDGAVAAAFTSERHLLVGGRAPETFAPLSRFWRTADGWVRTHANYPHHRARLLAALDVPGDPDAVAAALAERSAREAEEAVYAAGGLAIALRTPEEWAAHPQGAEVAKRPLVERERLDSAAARDLPPLGTGAGPLLPAAGLRVLDLTRVIAGPVATRTLALLGADVLRLDAPGLPELPGQHADTGFGKRSALLDLTADREVFEELLATADVVVTGYRPGALDRLGLTAGALAERRPGVVVAQLSAWGAYGPWGGRRGFDSLVQVATGIAATEGSAAGRPGVLPAQALDHGTGYLLAAGVLRAVGEQVEAGGSRVVRVALARTAGWLLAGASRGDGVSSSGDVAVGGWLSERESALGRLRYALSPVVFEGGPRDWGRGPGGWGADPARWV

Crispr_ID: CP023407_4

• CRISPR_ID: CP023407_4
• CRISPR_location: 2730818-2730914
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 1 spacers

Consensus repeat of CP023407_4

Consensus_repeat	Method
TGACATGAGCCTCCGCGCCCCGA	CRISPRCasFinder

spacers of CP023407_4

>4.1|2730841|51|CP023407|CRISPRCasFinder
CACCACACCGGCAGCCACCGGGACATCGCCGCCACGCGCACGGGAGGCGAG

CRISPR arrays and Neighbor proteins around CP023407_4

CRISPR arrays

The CRISPR arrays of CP023407_4

>merge|CP023407|4|2730818-2730914|CRISPRCasFinder
TGACATGAGCCTCCGCGCCCCGACACCACACCGGCAGCCACCGGGACATCGCCGCCACGCGCACGGGAGGCGAGTGACATGAACCTCCGCGCCCCGA

>CP023407|4|3|2730818-2730914|CRISPRCasFinder
TGACATGAGCCTCCGCGCCCCGA	CACCACACCGGCAGCCACCGGGACATCGCCGCCACGCGCACGGGAGGCGAG
TGACATGAACCTCCGCGCCCCGA

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
CP023407.1|AYL36124.1|2723169_2724519_+|hypothetical-protein	unknown	unknown	gnl|CDD|375997
CP023407.1|AYL39466.1|2716614_2717607_+|sugar-binding-protein	unknown	unknown	gnl|CDD|270408
CP023407.1|AYL39467.1|2721557_2723057_+|serine/threonine-protein-phosphatase	unknown	unknown	gnl|CDD|223704
CP023407.1|AYL36138.1|2742657_2743128_-|single-stranded-DNA-binding-protein	unknown	unknown	gnl|CDD|236092
CP023407.1|AYL36131.1|2734894_2735560_-|hypothetical-protein	unknown	unknown	unknown
CP023407.1|AYL36136.1|2740378_2740924_+|hypothetical-protein	unknown	unknown	unknown
CP023407.1|AYL36134.1|2737860_2738394_+|hypothetical-protein	unknown	unknown	gnl|CDD|380121
CP023407.1|AYL36129.1|2731103_2733854_+|hypothetical-protein	unknown	unknown	gnl|CDD|225669
CP023407.1|AYL36137.1|2740963_2742379_-|peptidase	unknown	unknown	gnl|CDD|369823
CP023407.1|AYL36126.1|2725937_2726942_-|methyltransferase	unknown	unknown	gnl|CDD|275041
CP023407.1|AYL36123.1|2718971_2721554_+|serine/threonine-protein-kinase	unknown	unknown	gnl|CDD|319004
CP023407.1|AYL36132.1|2735556_2735973_-|thioesterase	unknown	unknown	gnl|CDD|223894
CP023407.1|AYL36122.1|2717650_2718949_+|hypothetical-protein	unknown	unknown	unknown
CP023407.1|AYL36135.1|2738968_2740177_-|hypothetical-protein	unknown	unknown	unknown
CP023407.1|AYL36127.1|2726991_2727405_+|globin	unknown	unknown	gnl|CDD|381280
CP023407.1|AYL36125.1|2724601_2725957_+|phosphopeptide-binding-protein	unknown	unknown	gnl|CDD|238017
CP023407.1|AYL36133.1|2735977_2737642_-|energy-dependent-translational-throttle-protein-EttA	unknown	unknown	gnl|CDD|236992
CP023407.1|AYL36121.1|2715082_2716534_+|hypothetical-protein	unknown	unknown	gnl|CDD|225499
CP023407.1|AYL36130.1|2733912_2734875_+|ABC-transporter	unknown	unknown	gnl|CDD|213222
CP023407.1|AYL36120.1|2713580_2714543_+|ATPase	unknown	unknown	gnl|CDD|225518

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
CP023407.1|AYL36124.1|2723169_2724519_+|hypothetical-protein	gnl|CDD|375997	pfam18571, VWA_3_C, von Willebrand factor type A C-terminal domain. This is the C-terminal domain of von Willebrand factor type A pfam13768.	2.49926e-18
CP023407.1|AYL39466.1|2716614_2717607_+|sugar-binding-protein	gnl|CDD|270408	cd13690, PBP2_GluB, Substrate binding domain of ABC glutamate transporter; the type 2 periplasmic binding protein fold. This group includes periplasmic glutamate-binding domain GluB from Corynebacterium efficiens and its related proteins. The GluB domain belongs to the type 2 periplasmic binding protein fold superfamily (PBP2), whose many members are involved in chemotaxis and uptake of nutrients and other small molecules from the extracellular space as a primary receptor. The PBP2 proteins are typically comprised of two globular subdomains connected by a flexible hinge and bind their ligand in the cleft between these domains in a manner resembling a Venus flytrap. After binding their specific ligand with high affinity, they can interact with a cognate membrane transport complex comprised of two integral membrane domains and two receptor cytoplasmically-located ATPase domains. This interaction triggers the ligand translocation across the cytoplasmic membrane energized by ATP hydrolysis.	6.59627e-83
CP023407.1|AYL39467.1|2721557_2723057_+|serine/threonine-protein-phosphatase	gnl|CDD|223704	COG0631, PTC1, Serine/threonine protein phosphatase [Signal transduction mechanisms].	2.17657e-38
CP023407.1|AYL36138.1|2742657_2743128_-|single-stranded-DNA-binding-protein	gnl|CDD|236092	PRK07772, PRK07772, single-stranded DNA-binding protein; Provisional.	2.85977e-23
CP023407.1|AYL36134.1|2737860_2738394_+|hypothetical-protein	gnl|CDD|380121	pfam18143, HAD_SAK_2, HAD domain in Swiss Army Knife RNA repair proteins. Family of HAD domain phophoesterases observed in large eukaryotic proteins with predicted role in RNA repair, the so-called 'Swiss Army Knife' repair proteins. May be involved in phosphate group removal during RNA re-ligation.	4.74996e-08
CP023407.1|AYL36137.1|2740963_2742379_-|peptidase	gnl|CDD|369823	pfam08341, TED, Thioester domain. This domain is found near the N-terminus of a variety of bacterial surface proteins and pili. This domain contains an unusual covalent ester bond between a conserved cysteine and glutamine residue.	2.50433e-14
CP023407.1|AYL36126.1|2725937_2726942_-|methyltransferase	gnl|CDD|275041	TIGR04188, conserved_hypothetical_protein, methyltransferase, ATP-grasp peptide maturase system. Members of this protein family are predicted SAM-dependent methyltransferases that regularly occur in the context of a putative peptide modification ATP-grasp enzyme (TIGR04187, related to enzymes of microviridin maturation) and a putative ribosomal peptide modification target (TIGR04186).	9.78803e-75
CP023407.1|AYL36123.1|2718971_2721554_+|serine/threonine-protein-kinase	gnl|CDD|319004	pfam16918, PknG_TPR, Protein kinase G tetratricopeptide repeat. This domain is found at the C-terminus of protein kinase G and contains a tetratricopeptide repeat (TPR).	5.36596e-92
CP023407.1|AYL36132.1|2735556_2735973_-|thioesterase	gnl|CDD|223894	COG0824, FcbC, Predicted thioesterase [General function prediction only].	4.97984e-30
CP023407.1|AYL36129.1|2731103_2733854_+|hypothetical-protein	gnl|CDD|225669	COG3127, COG3127, Predicted ABC-type transport system involved in lysophospholipase L1 biosynthesis, permease component [Secondary metabolites biosynthesis, transport, and catabolism].	0.000183832
CP023407.1|AYL36127.1|2726991_2727405_+|globin	gnl|CDD|381280	cd14771, TrHb2_Mt-trHbO-like_O, Truncated hemoglobins, group 2 (O); Mycobacterium tuberculosis hemoglobin O like. The M- and S families exhibit the canonical secondary structure of hemoglobins, a 3-over-3 alpha-helical sandwich structure (3/3 Mb-fold), built by eight alpha-helical segments. Truncated hemoglobins (TrHbs, 2/2Hb, or 2/2 globins) or the T family globins adopt a 2-on-2 alpha-helical sandwich structure, resulting from extensive and complex modifications of the canonical 3-on-3 alpha-helical sandwich that are distributed throughout the whole protein molecule. TrHbs are classified into three main groups based on their structural properties and named after Mycobacterium sp. genes glbN, glbO, and glbP: TrHb1s (N), TrHb2s (O) and TrHb3s (P). This group includes a Mycobacterium tuberculosis TrHb2, Mt-trHbO, encoded by the Mycobacterium tuberculosis glbO gene, which is expressed throughout the Mycobacterium growth phase. It also includes a TrHb2 from the thermophilic Thermobifida fusca ( Tf-trHb) which has a high thermostability and at the optimal growth temperature for Thermobifida fusca (between 55 and 60 degrees C ), it is capable of efficient O2 binding and release. Tf-trHb shares a relatively slow rate of oxygen binding with Mt-trHbO.	2.23303e-68
CP023407.1|AYL36125.1|2724601_2725957_+|phosphopeptide-binding-protein	gnl|CDD|238017	cd00060, FHA, Forkhead associated domain (FHA); found in eukaryotic and prokaryotic proteins. Putative nuclear signalling domain. FHA domains may bind phosphothreonine, phosphoserine and sometimes phosphotyrosine. In eukaryotes, many FHA domain-containing proteins localize to the nucleus, where they participate in establishing or maintaining cell cycle checkpoints, DNA repair, or transcriptional regulation. Members of the FHA family include: Dun1, Rad53, Cds1, Mek1, KAPP(kinase-associated protein phosphatase),and Ki-67 (a human nuclear protein related to cell proliferation).	5.71227e-20
CP023407.1|AYL36133.1|2735977_2737642_-|energy-dependent-translational-throttle-protein-EttA	gnl|CDD|236992	PRK11819, PRK11819, putative ABC transporter ATP-binding protein; Reviewed.	0
CP023407.1|AYL36121.1|2715082_2716534_+|hypothetical-protein	gnl|CDD|225499	COG2948, VirB10, Type IV secretory pathway, VirB10 components [Intracellular trafficking and secretion].	0.000208426
CP023407.1|AYL36130.1|2733912_2734875_+|ABC-transporter	gnl|CDD|213222	cd03255, ABC_MJ0796_LolCDE_FtsE, ATP-binding cassette domain of the transporters involved in export of lipoprotein and macrolide, and cell division protein. This family is comprised of MJ0796 ATP-binding cassette, macrolide-specific ABC-type efflux carrier (MacAB), and proteins involved in cell division (FtsE), and release of lipoproteins from the cytoplasmic membrane (LolCDE). They are clustered together phylogenetically. MacAB is an exporter that confers resistance to macrolides, while the LolCDE system is not a transporter at all. An FtsE null mutants showed filamentous growth and appeared viable on high salt medium only, indicating a role for FtsE in cell division and/or salt transport. The LolCDE complex catalyzes the release of lipoproteins from the cytoplasmic membrane prior to their targeting to the outer membrane.	3.45007e-87
CP023407.1|AYL36120.1|2713580_2714543_+|ATPase	gnl|CDD|225518	COG2971, COG2971, Predicted N-acetylglucosamine kinase [Carbohydrate transport and metabolism].	1.65594e-27

Protein sequences

>CP023407.1|AYL36127.1|2726991_2727405_+|globin
MDGVTEIRRGTLQEQTFYEQVGGEETFRRLVHRFYEGVAGDPLLRPMYPEEDLGPAEERLVLFLMQYWGGPTTYSENRGHPRLRMRHVPFKVDRAAHDAWLKHMRDAVDELGLSEEHEQTLWKYLTYAAASMINAPD
>CP023407.1|AYL36126.1|2725937_2726942_-|methyltransferase
MGGQEPDTRDGLAASARAGLVREIDASGAFDADPVWREAFAAVPRHLFVPYYYVGVVGGYERRWGESPDPAARERWLRGAYEDTPLAIRLRDGELLSSSSQPSLMALMLAGLGLRDGQKVLEIGTGSGYNAALLAHRLGDDDLVTTVDLDPDITESARRHLDAAGHRPAVVTGDGARGVPARAPFDRIIATCALPSVPRAWPAQCVPGARILAPFATGLVVLTVRDAGHAEGRFLHTPAYFVPLRGASRSWQEPPDLEGLPAAARGSDLFRFLLALTAGALEPREAYGVWEREGRPQRERYGLTVDDEGDRAWLDDPAGPHVWPVPGSLSPGGS
>CP023407.1|AYL36125.1|2724601_2725957_+|phosphopeptide-binding-protein
MPTCPNGHQSGSDDWCEVCGHRMAGAVPPPPPPPPAPGGGYGFPPPGGPAGGPGGFGGPGGAQPQFCPQCRTPREGAAPFCEECRWNFLTNTATSYTPAAPRPPAPGPGPGAGPGPGPGGPFQQPPGGPSYGGDSYDYQGSRPSQVNRPAEPIPPGPPGFGGDPSRPVPPPPGPGGPGGPGGFGGPGGYGGPNGPGGPGGPGGPGGPGRPGGPGGPGGPHGPGAPQAFQSGPPAPQVFPQDTGRPQPGGPASGGDDDWVISPPTGGPGGPGGPGPGGPGGPNGPGPRGGYGYPQPGATQAPPGPGGFPQQPRPPQGPATWTATIGPDREYFMAMMHRSGPEAAGLNLPAYSPEQQRTLTGNQITIGRRRHSTGDTPDIDLSTPPEDPGVSHQHAVLVQQPDGTWAVVDQNSTNGTTVNGSDEPIQPFVPVPLQDGDRVHVGAWTTITIHRG
>CP023407.1|AYL36124.1|2723169_2724519_+|hypothetical-protein
MANFSKSNVPQFSVDVYQNAYLPEGGREVNAIVTVTATGGGTIGSAVTAPHLYSPGEGPSAAVVLMVDCSGSMDYPPTKMRNARDATAAAIDALRDGVRFAVVGGTHVAKEVYPGGGGLAVAGPGTREQAKQALRKLSAGGGTAIGTWLGLADRLLASADVAIRHGILLTDGRNEHESQEDLKAALEACAGRFTCDARGVGTDWEVKEVTGVASALLGTADIVADPAGLAADFTRMMETAMGKEVADVSLRLWTPVGTTVRFVKQVAPTVEELTGRRTEAGPRAGDYPLGSWGDESRDYHLCVEVPPAGVGQEMLAARLSLVIPRPGEETARNLGAQGLVRAVWTDDMAASTSINPQVAHYTGQAELAEVIQQGLDLRKEGDMDGATAKLGRAVQLASASGNADTAKLLAKVVDVVDAATGTVRLKAKVEEADEMTLETRSTKTVRVKK
>CP023407.1|AYL39467.1|2721557_2723057_+|serine/threonine-protein-phosphatase
MSQMPQQAALPKCPSCEEPLESGDRFCGACGYDLSAVPARPRDEPTIAMNGTVPAQAPADHGVPRPPAAPPAAPAPPAAPPAAAAWPAPDGPADPEPAHRPADVPGTNSDGSPLSGAPAAPQVPAQDTPVRPSPAEGAPVPPPPPPAAPSSGVRFDRRAQPDGQAADGPAGQPDEYPLQAPDPRVAAEPAGAGSAAAVCVACRAGRVDDDGYCENCGHAQPRERDHMEQECGPLAAVSDRGLRHHRNEDAFGVGTTALPDGSPVSVAIVCDGVSSATRPDEASLAASRAANESLLAALPRGTHPQQAMHDAIVAAAEAVDALAGEPAAARERDPHENAPACTIVGAVVTAGLLVVGWVGDSRAYWVPVDRSAPAARLTEDDSWAAQMVAAGLMGEAEAYADQRAHAITGWLGADAYELEPHTASFKPDRPGVVVVCTDGLWNYAESADEMAGVVPEDAAVRPLHGARVLVGHALDGGGHDNVTVALVPFPAVPQGAGSA
>CP023407.1|AYL36123.1|2718971_2721554_+|serine/threonine-protein-kinase
MSEPGSAPGGTPGNARTGQAARSACQRPDCGGAYEDVGGGELYCDTCGLAPVVSPTGMVASPPTGVTAHGRDSRGSGSTGAGGHSGRSGRSSRTSSQSSKSRRSVSGRLSRSLSGRTTGRSVSVRSSGAGAGPSGRGRLGAGLVEVPPIPRPDPRVMVLDNPEVPERKRFCSRADCGAQVGRARGGRPGRTEGFCTKCGHPYSFVPKLRDGDVVHGQYEVAGCLAHGGLGWVYLAVDRAVSDRWVVLKGLLDTGDQDAMAAAISERRFLAEIEHANIVRIYNFVEHLDQRTGSLDGYIVMEYLGGKSLKEIANGRRTPAGRRDPLPVEQACAYGIEALEALAHLHSRNLLYCDFKVDNAIQTEDQLKLIDMGAVRRMDDEESAIYGTVGYQAPEVADAGPSVASDLYTVARTLAVLTFDFQGYTNVYVDSLPDPDNIEVFRQYESFYRLLVRATDPDAARRFASAQEMTEQLTGVLREVVSLQSGRARPALSTLFGPETRVTDTVLFPALDGDVSRLGTRPGSRAVRRSRGGTPPALPPAAGGGPALVAPVDCAAAALALPVPLVDPSDPNAGFLAGLLTSAPGELINALAAAPAPSAETRLREVRARLETGEWPAALAVLGTLEQQDPDDWRVVWYRGVAALVTGDHEGAALAFDAVYDAFPGEIAPKLALGVCAEVLGQLDNAAEYYRLVWATDPSCVSAAFGLARVRLAAGDRGGAVRTLESVPESSIHYTAARVAAVRARLRGRTAADGDVPFLDDLSAAAGQVEALEAYGLDPARRELLSAEVLGCGLDWVLSGGQGSALPAAGGRTLLGSGLDERGLRFGLERSYRALARLARSGEERIDLVERANRYRPRTWV
>CP023407.1|AYL36122.1|2717650_2718949_+|hypothetical-protein
MADTDPTGPVMDRDEVDRALARLGAEHEAIETSLLALQDHAGRRLLEGARLTGLTEERWTAAEASITLLWTCFDAYTDALRTARDIRARRRWSSREDLVELTELLRGDPVALAGGSPGKLSERYSLAALVDRMNELYASSLDMVVAADAVWSALPARIDLLAAELQRTSRLAHSVGVRPGEHPAGDDLERITRTLTRLREQVVSDPLAYWKRAEGSSAPGGGRPDTTVYDREARALEEVRREIDAVLTVRQDAEKRIVRLRDVLSRADRTLAEARTARGEVLAKIAATEVPVVSGPPTALQEQLSTAAEYRRHAQWHRLSPLLEALEQQAEDELMRARESLTAVTAPLAVRAELRGRLDAYKAKVARHGLAEDPVLVERYEKARRMLWSAPCDLRAADRAVLRYQQGAAELLGTAPRLPGQGVPDDRREPGA
>CP023407.1|AYL39466.1|2716614_2717607_+|sugar-binding-protein
MGAMAAACALALIFALALTRAAPAGPRATPAGQPVSQAAGTDAAEDCDAPEKQTLSPSDADGRTIEEIKNRKGEKRKLIVGVDQNSYRWGYRDPNGGESGTLEGFDIDLVHRIAEDILGDRDAVQFKAIPTDQRIPAIQAGRVDMVVRTMTINCDRIKDVAFSAPYFKTGQQVLAPKSAPIEGYDETLADQEICTATGSTAYAKLEADKKAGRLPESTGIGTTVPNQLDCLVRLQLGEVDAVVTDGALAASQAAQDPTVELKGEAFTTEYYGVAMRKDADDMVRRVNRILVDFREDADGWQASYTKWLSATLKGDPSRSEPPAPEYLREN
>CP023407.1|AYL36121.1|2715082_2716534_+|hypothetical-protein
MTYTPHNTAPPPGTAATTMPAVPPQPRPAPTPPPTPASTPPQEPETRRTAFAEGADRLRRAATTEPGRLRIIGALLAALVVAFGAVAAWQMTDRAAAADDVLTSSQPLSSDAADIYRSLADANTAASSGFLAGGQETAASRDRYEKDIRTAAAKLVNAAANSEPDSPSAETIAELNRLLPEYKGLVERARTYNRQGFPVGGAYLRYANEKMQEQMLPAAEDLYTTENQRLRSDYADATPYPWAAMALGVVALTALAWAMRRNYLRTNRVLNHGLVAATTAATVVLLWLTIGHTLARSGLNDSYDNGVRSLNVLHDARIASLKARGNENLTLVARGAETTKVGDETFDAYDYDFDRDMDTLGKGLALAQRLADDDSGAEPVSSAAGNMTEWKKRHEAAREQDENGNYQQALDMVIGAEGATGECFDGVDENLATALAHEQDEFRRAAGDGRDALTGLPAGAAGLAVLGAAGAVLGIGRRLSEYR
>CP023407.1|AYL36120.1|2713580_2714543_+|ATPase
MGLILAVDAGNSKTDVAVVNHDGEVLATARGEGFRPPAVGLDTAMSALTATVTRALATAGTPTVSHVSACLANADLPIEEEQLTAALTHRGWGTTVDVRNDTFAILRAGVTEPRGVAVVCGAGINCVGMHPDGRTARFPALGRFSGDWGGGWALAEEALFHAARASDGRGEPTALAHALPRHFGLPDMPALIEALHLGHVAPARRHELAPVLFAVAATGDGVALTIIDRQAEEIVTMAVVALTRLGLLDEPTPVLLGGGVLAARDPRLEGRIRALLAERAPEAEPRVVTSRPVLGAALLGLDRMGAAGEAQGRVRTYFAT
>CP023407.1|AYL36129.1|2731103_2733854_+|hypothetical-protein
MSVRDPGRPQVNAPWVRTRLRAAPGGALALALLVAATAFLAAALPLAVDRYGDAGLRRSMEAAGPARTTVQLYSTALSDLALPPEERAARVREEAVRDTYTKIFPLPGRSLPLDPDQSAYGVRTSRPLEVQDPWLAMPDAVPPQFHLVAQGGLAGHSRLREGRLPKAPDDVTTAATEVEAAVSAETARRLHIEVGSVLHVPAVERAPLAVRVTGIVDPRAPRDAYWSANSVLRTPYLTNVPGDPERNVFWTGALLLPPDAAPATLSTAGEPERYWLLAPDAAALHGYDLPRMTSAVASLEAGPLLQQAGTAASGVVESEAALDEVFAEYTELRSAIGPLVAVAAFGTGTVAAVVLLMAGGLTADRRRPELTLLRARGASLRGLAGRLLAETAVVAVPAAALGLAAALLALPGARPASAVAAAGAVALLACAALPLRAVAAHRAVRVHAARDDIASARPSRRRTVAELTLLVLAVGAVVALRTRGTSDGAGASGDELVSLAPVLVGMIAALVLVRLYPLPLRGLARPAGRLRGVVGPLSLARAGRASGSAVLPLLALLTALTTAAFGGSVLTGVADARERAAVLTVGADARVEAVQALPSGLAGRIGRVPGVRDVTALSVFPYAQPEGGRLAVPVAGVDPAGYAALTRHTGVGAFPADALKSSGGKDLAALASPAVADRYGTRPFLVRLDDGGIVTVRITAVRERTPAVAGEEFLVVDRSGLPGESARPTTLLVTGDPADAGALREAAGGGAQVQLRTERFAEYVDSPLQSGAERVYTAAVAAGAGFAMLSLLLTLLRAAPERAALLARLRTMGLTRAQGRRLLVLESLPQALLAAAGGTVTAWAAIRLLSSGIDLTTVAVSASASPAGRAALRTDPVSLLVPALAVVAVTVGTAAAQAWWTGRRGSVRELRAGDAR
>CP023407.1|AYL36130.1|2733912_2734875_+|ABC-transporter
MTSHPTLAELAGRVTADRDRPAYGHDALITCDRLVRVFTADGVEVQALQGLDLLVREGELMALVGASGSGKSTLMNILAGLDTPTAGAARVAGRDLLTMTAKDRLAYRREVVGFVWQQTSRNLLPYLTSAQNVALPMRLAGGRSRSARRARAERVLELLELLEVADCRDRQPHQMSGGQQQRVAIAVALAGGPSVLLADEPTGELDSHTAEQIFAAFRTANERLGTTIVIVTHDQAVAGEVRRTVAIRDGRTSTEVLRRSEVDAATGDETVVAREYAMLDRAGRLQLPAEYTRALDMRDRVALELEPDHIAVRPDDSRRD
>CP023407.1|AYL36131.1|2734894_2735560_-|hypothetical-protein
MTVLHLADEGEAADLAAFLSRLLHYDRSAAVRLQAAGTALAVFGRPPSFEVLAIRAVRLAKPYENGLDVTLDVTVSAGELLESVDEAGATAAVPGAVTGPPWAGVLPPRGGWRAEPGLPGPQELRGLVAASVAEFRSRTGQLEPSSRTRAELDRIGREIWSRTVAETGLPVRAVHAAQSFRFLRGAAPGLFSSGSWLRLRTPYGSVAVRRAGLGALGLSVR
>CP023407.1|AYL36132.1|2735556_2735973_-|thioesterase
MRHIYRCPLRWADMDAYGHVNNVVFLRYLEEARIDFLFRPDKDFKQGSVVSRHEIDYKRQLVHRHSPVDIELWVTEIRAASFTLTYEVKDDDLVYVRASTVIVPFDFAAQRPRRITDEEREFLEEYMDAQDKEEAVAA
>CP023407.1|AYL36133.1|2735977_2737642_-|energy-dependent-translational-throttle-protein-EttA
MAEFIYTMRKARKAHGDKVILDDVTLSFLPGAKIGVVGPNGAGKSTVLKIMAGLEQPSNGDAFLTPGYTVGILLQEPPLNEEKTVLENVQEGVAEVKGKLDRFNEIAEQMATEYTDELMDEMGKLQEQLDHANAWDLDAQLEQAMDALGCPPGDWPVSNLSGGEKRRVALCKLLLEAPDLLLLDEPTNHLDAESVNWLEQHLAKYEGTVVAVTHDRYFLDNVAGWICEVDRGRLHGYEGNYSKYLETKAARLKVEGAKDAKRQKRLKDELEWVRSNAKGRQAKSKARLARYEEMAAEADKMRKLDFEEIQIPPGPRLGSIVVEVNNLSKGFGEKLLIDDLSFTLPRNGIVGIIGPNGAGKTTLFKMIQGIEEPDAGSIKVGETVKISYVDQSRENIDPKKTLWAVVSDELDYINVGQVEMPSRAYVSAFGFKGPDQQKPAGVLSGGERNRLNLALTLKQGGNLLLLDEPTNDLDVETLSSLENALLEFPGCAVVVSHDRWFLDRVATHILAYEGESKWFWFEGNFESYEKNKVERLGADAARPHRATYKKLTRG
>CP023407.1|AYL36134.1|2737860_2738394_+|hypothetical-protein
MTSAAERPLLFLDVDGPLIPFGSPSGHPPSAAAADRGNPLLARLDPGVGARFMALGCPLVWATTWMEEANRTVAPRIGLPKLPVLEWPDADAEEGPRGLHWKTRPLVEWAAGRPFIWVDDEISTIDRLWVAAAHPGPSLLHQVDPAKGLEDSDFHTLTGWLDAASGPGGSAASPPLT
>CP023407.1|AYL36135.1|2738968_2740177_-|hypothetical-protein
MHGRVHDVVWGTVRVVLAVLLLGAAWQSARSAERERSDDLDFARLAIHDAQGRDTGSLKVCGDRYREPLCLRAEHVAVDEVLLDMNRKSGARYEFLFGRSGGRTLALTLNIRHADQDLVEAVGRNGEATLYWWRDSVRLLKVSTDGEQRTLRTAHHPGREFVPPAAFGAVLAGLGAAALWAGLWRIARGGVSRLSWPWQTTVPGVSFTLAGLGGALGALFAVRSPWTMAWTAGVVGAVSAVGMACWAYRLVRRRLPALERMEPVAPSAERDFPGTVCGPGPWQLLSMGWLRTGPRGLAAVPGPATGAGSFGVRPFPGALRLVRVRPPHRDDPLRLRFGRFRPTDGGKGSQFPLVAECEALGEPVPGARILIGAGSDDLPHILGALRPDPEPAPPRGRETSPT
>CP023407.1|AYL36136.1|2740378_2740924_+|hypothetical-protein
MDKAAPLRGGAVLVAAVAAAAVLTAPAVGDEAEARGGKAAETRQIASSVLDAEQDVKFTLTAVRSADDPLMASVRLKAFARQHGRFVLTDEVRVGAVDGWFWFPVTGRGAVCEFSTASTDPAPLAVSLLITPSIGCSAPERFELRDGRFGGGDRGARTPHGGVRTGGGGLAGRAAAPPERT
>CP023407.1|AYL36137.1|2740963_2742379_-|peptidase
MFASFSAWSARGRGSARLAAGALVSGLVAATALAGAGTAVADSDGTAPGHGGAAATLGGLKTYGEAVIRGAAGDQRVSAGLFEMSVEGGGMLQTYCVDIQTPTQKDAKYHETAWSGTSLGTNKDAGRIRWMLQNSYPQVNDLAALADKAGVRGRLTEQDAAAGTQVAIWRYSDDVDVDAVDPQAEQLADYLEKNARGQAEPKASLTLDPPAVSGRPGERIGPVTVRTDARSVTLSPPADATTTGVRIVDRDGEPVTTATDGGKVYFEVPEDAAADTAELIAQASTTVPVGRAFASDSRSQTQILAGSSESTVSATATATWALEGAIPALSARANCAKGGVDVTVANRDDEPFTFELTGAEYTVAGGASRTVTVPLSEDQPYDFTVEGPDGFERRFTGMLDCRTRAAETGTTTQTLTGPSPTALAAPATDTNLAETGASGATPWIAGTAIGLVVLGGAGLVVARRRQTPPGA
>CP023407.1|AYL36138.1|2742657_2743128_-|single-stranded-DNA-binding-protein
MNETMVCVVGNVATRPVYRETASGPAARFRLAVTSRYWDREKSAWADGHTNFFTVWANRQLATNTAASLSVGEPVIVQGRLKIRSETREGQQGWMSADVDAVSIGHDLARGTTAFRRAQRPEQTSGPDRPEPNWESKPEGSQTATPQQHAQATVVT

Crispr_ID: CP023407_6

• CRISPR_ID: CP023407_6
• CRISPR_location: 4838926-4839023
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 1 spacers

Consensus repeat of CP023407_6

Consensus_repeat	Method
GCCCTGCGGCACGACTGCCCGCGG	CRISPRCasFinder

spacers of CP023407_6

>6.1|4838950|50|CP023407|CRISPRCasFinder
GAACGGCGGGGCGGCTGCGCGCGGAAACGGCGGGGCGGGGGTGGGCTGCT

CRISPR arrays and Neighbor proteins around CP023407_6

CRISPR arrays

The CRISPR arrays of CP023407_6

>merge|CP023407|6|4838926-4839023|CRISPRCasFinder
GCCCTGCGGCACGACTGCCCGCGGGAACGGCGGGGCGGCTGCGCGCGGAAACGGCGGGGCGGGGGTGGGCTGCTGCTCTGCGGCACGACTGCCCGCGG

>CP023407|6|5|4838926-4839023|CRISPRCasFinder
GCCCTGCGGCACGACTGCCCGCGG	GAACGGCGGGGCGGCTGCGCGCGGAAACGGCGGGGCGGGGGTGGGCTGCT
GCTCTGCGGCACGACTGCCCGCGG

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
CP023407.1|AYL37883.1|4844738_4845608_+|biotin--[acetyl-CoA-carboxylase]-ligase	unknown	unknown	gnl|CDD|223417
CP023407.1|AYL37880.1|4842576_4842705_-|hypothetical-protein	unknown	unknown	unknown
CP023407.1|AYL37875.1|4836185_4837634_+|NAD(P)H-quinone-dehydrogenase	unknown	unknown	gnl|CDD|236112
CP023407.1|AYL37874.1|4835648_4836089_-|gamma-glutamylcyclotransferase	unknown	unknown	gnl|CDD|372719
CP023407.1|AYL37884.1|4846376_4847483_+|adenylate/guanylate-cyclase-domain-containing-protein	unknown	unknown	gnl|CDD|143636
CP023407.1|AYL39627.1|4849185_4850304_+|diguanylate-cyclase	unknown	unknown	gnl|CDD|128563
CP023407.1|AYL37870.1|4830513_4831467_-|deoxyribose-phosphate-aldolase	unknown	unknown	gnl|CDD|188646
CP023407.1|AYL37882.1|4843038_4844628_-|methylmalonyl-CoA-carboxyltransferase	unknown	unknown	gnl|CDD|227136
CP023407.1|AYL37885.1|4848280_4849081_+|enoyl-CoA-hydratase	unknown	unknown	gnl|CDD|181069
CP023407.1|AYL37876.1|4837780_4838746_+|DeoR-family-transcriptional-regulator	unknown	unknown	gnl|CDD|224268
CP023407.1|AYL37869.1|4828176_4829046_-|aldehyde-dehydrogenase	unknown	unknown	gnl|CDD|365919
CP023407.1|AYL37881.1|4842812_4843022_-|hypothetical-protein	unknown	unknown	gnl|CDD|379386
CP023407.1|AYL37868.1|4827771_4828110_+|hypothetical-protein	unknown	unknown	unknown
CP023407.1|AYL37873.1|4834732_4835557_-|purine-nucleoside-phosphorylase	unknown	unknown	gnl|CDD|236183
CP023407.1|AYL37878.1|4841393_4841921_+|hypothetical-protein	unknown	unknown	gnl|CDD|369035
CP023407.1|AYL39626.1|4829056_4830502_-|betaine-aldehyde-dehydrogenase	unknown	unknown	gnl|CDD|143429
CP023407.1|AYL37879.1|4841892_4842510_-|septum-formation-inhibitor-Maf	unknown	unknown	gnl|CDD|178901
CP023407.1|AYL37872.1|4832400_4834035_-|phosphomannomutase	unknown	unknown	gnl|CDD|100092
CP023407.1|AYL37871.1|4831579_4832320_+|hypothetical-protein	unknown	unknown	gnl|CDD|371225
CP023407.1|AYL37877.1|4839256_4841029_-|acetyl-/propionyl-CoA-carboxylase-subunit-alpha	unknown	unknown	gnl|CDD|227111

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
CP023407.1|AYL37883.1|4844738_4845608_+|biotin--[acetyl-CoA-carboxylase]-ligase	gnl|CDD|223417	COG0340, BirA, Biotin-(acetyl-CoA carboxylase) ligase [Coenzyme metabolism].	2.46539e-55
CP023407.1|AYL37875.1|4836185_4837634_+|NAD(P)H-quinone-dehydrogenase	gnl|CDD|236112	PRK07845, PRK07845, flavoprotein disulfide reductase; Reviewed.	0
CP023407.1|AYL37874.1|4835648_4836089_-|gamma-glutamylcyclotransferase	gnl|CDD|372719	pfam13772, AIG2_2, AIG2-like family. This family is found in bacteria and metazoa.	2.52628e-23
CP023407.1|AYL37884.1|4846376_4847483_+|adenylate/guanylate-cyclase-domain-containing-protein	gnl|CDD|143636	cd07302, CHD, cyclase homology domain. Catalytic domains of the mononucleotidyl cyclases (MNC's), also called cyclase homology domains (CHDs), are part of the class III nucleotidyl cyclases. This class includes eukaryotic and prokaryotic adenylate cyclases (AC's) and guanylate cyclases (GC's). They seem to share a common catalytic mechanism in their requirement for two magnesium ions to bind the polyphosphate moiety of the nucleotide.	3.12493e-28
CP023407.1|AYL39627.1|4849185_4850304_+|diguanylate-cyclase	gnl|CDD|128563	smart00267, GGDEF, diguanylate cyclase. Diguanylate cyclase, present in a variety of bacteria.	3.60552e-38
CP023407.1|AYL37870.1|4830513_4831467_-|deoxyribose-phosphate-aldolase	gnl|CDD|188646	cd00959, DeoC, 2-deoxyribose-5-phosphate aldolase (DERA) of the DeoC family. 2-deoxyribose-5-phosphate aldolase (DERA) of the DeoC family. DERA belongs to the class I aldolases and catalyzes a reversible aldol reaction between acetaldehyde and glyceraldehyde 3-phosphate to generate 2-deoxyribose 5-phosphate. DERA is unique in catalyzing the aldol reaction between two aldehydes, and its broad substrate specificity confers considerable utility as a biocatalyst, offering an environmentally benign alternative to chiral transition metal catalysis of the asymmetric aldol reaction.	2.23528e-76
CP023407.1|AYL37885.1|4848280_4849081_+|enoyl-CoA-hydratase	gnl|CDD|181069	PRK07657, PRK07657, enoyl-CoA hydratase; Provisional.	6.26412e-75
CP023407.1|AYL37876.1|4837780_4838746_+|DeoR-family-transcriptional-regulator	gnl|CDD|224268	COG1349, GlpR, Transcriptional regulators of sugar metabolism [Transcription / Carbohydrate transport and metabolism].	3.03889e-81
CP023407.1|AYL37869.1|4828176_4829046_-|aldehyde-dehydrogenase	gnl|CDD|365919	pfam00171, Aldedh, Aldehyde dehydrogenase family. This family of dehydrogenases act on aldehyde substrates. Members use NADP as a cofactor. The family includes the following members: The prototypical members are the aldehyde dehydrogenases EC:1.2.1.3. Succinate-semialdehyde dehydrogenase EC:1.2.1.16. Lactaldehyde dehydrogenase EC:1.2.1.22. Benzaldehyde dehydrogenase EC:1.2.1.28. Methylmalonate-semialdehyde dehydrogenase EC:1.2.1.27. Glyceraldehyde-3-phosphate dehydrogenase EC:1.2.1.9. Delta-1-pyrroline-5-carboxylate dehydrogenase EC: 1.5.1.12. Acetaldehyde dehydrogenase EC:1.2.1.10. Glutamate-5-semialdehyde dehydrogenase EC:1.2.1.41. This family also includes omega crystallin, an eye lens protein from squid and octopus that has little aldehyde dehydrogenase activity.	1.01221e-39
CP023407.1|AYL37881.1|4842812_4843022_-|hypothetical-protein	gnl|CDD|379386	pfam13822, ACC_epsilon, Acyl-CoA carboxylase epsilon subunit. This family includes the epsilon subunits of propionyl-CoA carboxylase, EC:6.4.1.3, and acetyl-CoA carboxylase, EC:6.4.1.2. These enzymes are involved in the biosynthesis of long-chain fatty acids. The epsilon subunit is necessary for an efficient interaction between the alpha and beta subunits of these enzymes.	2.80058e-08
CP023407.1|AYL39626.1|4829056_4830502_-|betaine-aldehyde-dehydrogenase	gnl|CDD|143429	cd07111, ALDH_F16, Aldehyde dehydrogenase family 16A1-like. Uncharacterized aldehyde dehydrogenase family 16 member A1 (ALDH16A1) and other related sequences are present in this CD. The active site cysteine and glutamate residues are not conserved in the human ALDH16A1 protein sequence.	0
CP023407.1|AYL37873.1|4834732_4835557_-|purine-nucleoside-phosphorylase	gnl|CDD|236183	PRK08202, PRK08202, purine nucleoside phosphorylase; Provisional.	1.00995e-130
CP023407.1|AYL37882.1|4843038_4844628_-|methylmalonyl-CoA-carboxyltransferase	gnl|CDD|227136	COG4799, COG4799, Acetyl-CoA carboxylase, carboxyltransferase component (subunits alpha and beta) [Lipid metabolism].	0
CP023407.1|AYL37878.1|4841393_4841921_+|hypothetical-protein	gnl|CDD|369035	pfam06682, SARAF, SOCE-associated regulatory factor of calcium homoeostasis. SARAF is as family of eukaryotic proteins embedded in the ER. SARAF is SOCE-associated regulatory factor, where SOCE is store operated calcium entry. ie a mechanism governing calcium homoeostasis in the cell and the mitochondria. SOCE involves the enabling of Ca2+ release-activated Ca2+ (CRAC) channels. SARAF is a single pass ER membrane protein whose systolic-facing domain is responsible for activity and whose luminary-facing domain carries out a regulatory function in conjunction with another membrane protein STIM, an ER single pass membrane protein that detects changes in ER Ca2+ levels through its EF-hand, conserved Ca2+ binding domain. STIM is the major target for SARAF regulation, and thus SARAF negatively regulates the SOCE entry of calcium into cells protecting them from overfilling.	0.00570242
CP023407.1|AYL37879.1|4841892_4842510_-|septum-formation-inhibitor-Maf	gnl|CDD|178901	PRK00148, PRK00148, Maf-like protein; Reviewed.	1.22339e-96
CP023407.1|AYL37872.1|4832400_4834035_-|phosphomannomutase	gnl|CDD|100092	cd05799, PGM2, This CD includes PGM2 (phosphoglucomutase 2) and PGM2L1 (phosphoglucomutase 2-like 1). The mammalian PGM2 is thought to be a phosphopentomutase that catalyzes the conversion of the nucleoside breakdown products, ribose-1-phosphate and deoxyribose-1-phosphate to the corresponding 5-phosphopentoses. PGM2L1 is thought to catalyze the 1,3-bisphosphoglycerate-dependent synthesis of glucose 1,6-bisphosphate and other aldose-bisphosphates that serve as cofactors for several sugar phosphomutases and possibly also as regulators of glycolytic enzymes. PGM2 and PGM2L1 belong to the alpha-D-phosphohexomutase superfamily which includes several related enzymes that catalyze a reversible intramolecular phosphoryl transfer on their sugar substrates. Other members of this superfamily include phosphoglucosamine mutase (PNGM), phosphoacetylglucosamine mutase (PAGM), the bacterial phosphomannomutase ManB, the bacterial phosphoglucosamine mutase GlmM, and the bifunctional phosphomannomutase/phosphoglucomutase (PMM/PGM). Each of these enzymes has four domains with a centrally located active site formed by four loops, one from each domain. All four domains are included in this alignment model.	0
CP023407.1|AYL37871.1|4831579_4832320_+|hypothetical-protein	gnl|CDD|371225	pfam10756, bPH_6, Bacterial PH domain. This domain has a bacterial type PH domain structure. This domain was previously known as DUF2581. This family is conserved in the Actinomycetales. Although several members are annotated as RbiX homologs, RbiX being a putative regulator of riboflavin biosynthesis, the function could not be confirmed.	0.000873831
CP023407.1|AYL37877.1|4839256_4841029_-|acetyl-/propionyl-CoA-carboxylase-subunit-alpha	gnl|CDD|227111	COG4770, COG4770, Acetyl/propionyl-CoA carboxylase, alpha subunit [Lipid metabolism].	0

Protein sequences

>CP023407.1|AYL37876.1|4837780_4838746_+|DeoR-family-transcriptional-regulator
MFAAERRQLILEMVRANGAVSLRELARVVQTSEVTVRRDVRALEAEGLLDRRHGGAVLPGGFTRESGFPQKSHLATAEKTAIADLAANLVEEGEAIVVGAGTTTQELARRLARVPGLTVVTNSLLVAQALAHANRVEVVMTGGTLRGSNYALVGSGAEQSLQGLRVSRAFLSGSGLTAERGLSTSNMLSASVDRALVQAAAEVVVLADHTKLGTDTMFQTVPTDLITRLVTDEPPAHDDRAGTELQALADQGVQIAVAGAGNEGGSSAGAAGGRQRRDVPLPGPRRQGPGGLRAAVGLGAAGEAVQGAERGAARVADLRRR
>CP023407.1|AYL37875.1|4836185_4837634_+|NAD(P)H-quinone-dehydrogenase
MEHVTRIVIIGGGPGGYEAALVAAQLGAEVTVVDCDGLGGASVLTDCVPSKTLIATAEVMTTFDSSYEELGIIVADDTPPMEQAARVVGVDLGKVNRRVKRLALAQSHDITASVTRAGARVLRGRGRLEGMQALDGSRKVVVRAADGTEETLTADAVLIATGGHPRELPDARPDGERILNWTQVYDLNELPEELIVVGSGVTGAEFAGAYQALGSKVTLVSSRDRVLPGEDPDAAAVLEDVFRRRGMNVMARSRAQSAKRVGDRVEVTLADGRVITGSHCLMAVGAIPNSEGMGLEEAGVRLRESGHIWTDKVSRTTAPGVYAAGDVTGVFALASVAAMQGRIAMYHFLGDAVAPLNLKTVSSNVFTDPEIATVGYSQADVDGGKIDARVVKLPLLRNPRAKMQGIRDGFVKIFCRPGTGIVVGGVVVSPRASELIHPISIAVDNNLTVEQIANAFTVYPSLSGSIAEVARQLHTRKADGEA
>CP023407.1|AYL37874.1|4835648_4836089_-|gamma-glutamylcyclotransferase
MSLYAAYAGHLDARLMSRRAPHSPLRATGWLNGWRLTFGGEHMGWEGALATLVEDPISQVFVALYDIAPMDEEALDRWEGAAGLDLYRRTRVRVHTLDGEEPAWTYVLNSYEGGLPSARYLGEIADAAESAGAPHDYVMELRKRPC
>CP023407.1|AYL37873.1|4834732_4835557_-|purine-nucleoside-phosphorylase
MNASLLPDDIQGDPYAAADAAAARLRELTGAETHDVALVMGSGWAPAVDALGTPEAELRVTELPGFPPPVVEGHGGTVRSYAIGAKRALVFLGRTHYYEGRGVAAVAHGVRTAVAAGAKTIVLTNGCGGLRAGMRPGQPVLISDHINLTATSPIVGANFVDLTDLYSPRLRALCKGIDPSLEEGVYAQFPGPHYETPAEIRMARVIGADLVGMSTVLEAIAAREAGAEVLGISLVTNLAAGMTGEPLNHEEVLQAGRDSATRMGSLLAQVLGRL
>CP023407.1|AYL37872.1|4832400_4834035_-|phosphomannomutase
MQDDALIAQAQAWLAEDPDPDTREELARLIDAGDHAELTARFSGTLQFGTAGLRGELGAGPMRMNRSVVIRAAAGLAAYLKKQGHTDGLVVIGYDARHKSRDFAEDTAAVMTGAGLRAAVLGRPLPTPVLAFAIRHLNAVAGVEVTASHNPPRDNGYKVYLGDGSQIVPPADAEIAAEIEAVASLATVPRPTEGPRTLDDGVLDAYLARTDAVLAKGSPRTARTVYTAMHGVGKDTLLAAFARAGFPTPDLVTEQADPDPEFPTVAFPNPEEPGAMDLAFAKARETDPDLVIANDPDADRCAAAVKDGADWRMLRGDEVGALLAAHLVRRGATGTFAESIVSSSLLGRIAEKAGLPHVETLTGFKWIARAEGLRYGYEEALGYCVDPDGVRDKDGITAALLLTELASELKEENRTLLDLLDDLAVEHGLHATDQLSVRVQDLSLIAAAMRRLRDQPPTELAGLRITRTEDLTEGTDTLPPTDGLRYTLDGARVIVRPSGTEPKLKCYLEIVIPVATKADLPAARTKATTLLDDIKRDLSRSAGI
>CP023407.1|AYL37871.1|4831579_4832320_+|hypothetical-protein
MTTPEHQPNDPEPTRPPGSAAPRYQDRVYRSPAALVGGVLLLALIGWLGIDAVVTGEGRTPWLALATMLLLVPLVVAYTLRPAVYVNDERLRVRNPFRVIELPWGQVASLRSGFSNEVFDESGTKYQLWALPVSLRARNKAVRAETRAAAQAARAARAGSGSEGSGSGAGSGARASAGRGGLGPGGVPSGPKRAESDRAMDEMREMHELRQSEEAAQGEVTVRWAYEIAGPAVVGAVLLAVLLGVG
>CP023407.1|AYL37870.1|4830513_4831467_-|deoxyribose-phosphate-aldolase
MPTTAPHALSDVTASGSTLRRFLHGLPGVDAVGLEARAASLGTRSIKTTAKAYAIDLAISMVDLTTLEGADTPGKVRALGAKAVHPDPTDRTTPATAAVCVYPDMVATAKQAVAGSTVKVASVATAFPAGRAPIAVKLADVREAVAAGADEIDMVIDRGAFLAGDYLKVYDEITAVKEACGTSARLKVIFETGELSTYDNIRRASWLGMLAGADFIKTSTGKVAVNATPANTLLMLEAVRDFRAQTGVQVGVKPAGGIRTTKDAIKFLVLVNETAGEDWLDNHWFRFGASSLLNDLLMQRQKLATGRYSGPDYVTVD
>CP023407.1|AYL39626.1|4829056_4830502_-|betaine-aldehyde-dehydrogenase
MEKRTPAFEYAPAPESRAVVDIAPSYGLFIDGEFTEAADGKVFKTVSPSTEEVLSEVAEAGEADVDRAVKAARKAFEKWSALPGAERAKYLFRIARIIQERSRELAVLETLDNGKPIRETRDADLPLVAAHFFYYAGWADKLDHAGYGANPRPLGVAGQVIPWNFPLLMLAWKIAPALATGNTVVLKPAETTPLSALFFADICRQAGLPRGVVNIVTGDGRAGAALIAHPDVNKVAFTGSTAVGKEIARTVAGTRKKVTLELGGKGANIVFDDAPIDQAVEGIVNGIFFNQGQVCCAGSRLLVQESIHDELLDSLKRRLSTLRLGDPLDKNTDIGAINSAEQLARITALADRGEAEGAERWSPACELPESGYWFAPTLFTGVTQAHTVARDEIFGPVLSVLTFRTPDEAVAKANNTPYGLSAGIWTEKGSRILAVANKLRAGVIWSNTFNKFDPTSPFGGYKESGFGREGGRHGLEAYLDV
>CP023407.1|AYL37869.1|4828176_4829046_-|aldehyde-dehydrogenase
MLKTYKLYVGGKFPRSESGRVYEVTDAKKNWLANAPLSSRKDARDAVVAARKAFGGWAGATAYNRGQILYRVAEMLEGRRDQYVREVAEAEGLSKSKAGDQVDAAIDRWVWYAGWTDKIAQVVGGGNPVAGPFFNLSSPEPTGVVAVLAPQESSFLGLVSVVAPVIATGNTAVVVASEKSPLPALSLGEVLATSDLPGGVVNILSGRTAEIAAPLAAHQDVNAIDLAGADEALAKELEIAAADNLKRVLRPQPVDNWSATPGTDRMTAFLETKTVWHPTGSLGASGSAY
>CP023407.1|AYL37868.1|4827771_4828110_+|hypothetical-protein
MARHRAPRTPVARRALTVLATAGVVLGAGAATAAAADSGGLADDAGHAVGAVADLKPNPLAGTGVDPLDNGVGTQVADFRSVDSREVTGPVAQAPSVGSVPVAGQAAGMLRS
>CP023407.1|AYL37877.1|4839256_4841029_-|acetyl-/propionyl-CoA-carboxylase-subunit-alpha
MRKVLIANRGEIAVRVARACRDAGIASVAVYADPDRDALHVRAADEAFALGGDTPATSYLDIDKVLKAAKESGADAIHPGYGFLSENAEFAQAVLDADLIWIGPPPQAIRDLGDKVAARHIAQRAGAPLVAGTPDPVSGAEEVVAFAEEHGLPIAIKAAFGGGGRGLKVARTLEEVPELYDSAVREAVAAFGRGECFVERYLDKPRHVETQCLADKYGNVVVVSTRDCSLQRRHQKLVEEAPAPFLSEAQVDELYSASKAILKEAGYVGAGTVEFLVGLDGTISFLEVNTRLQVEHPVTEEVAGIDLVREMFRIADGEELGYGDPVLRGHSFEFRINGEDPGRNFLPAPGTVTTFAAPSGPGVRLDAGVESGSVIGPAWDSLLAKLIVTGRTRKEALERAARALNEFQVEGMATAIPFHRAVVRDPAFAPELTGSADPFTVHTRWIETEFVNEIKPFAAAVDAGAEDEADRETVVVEVGGKRLEVSLPASLGMSLARTGLAAGAKPKRRAAKKSGPVASGDTLASPMQGTIVKLAVEEGQEVKEGDLVVVLEAMKMEQPINAHRSGTIKGLSAEVGASVTSGAAICEIKD
>CP023407.1|AYL37878.1|4841393_4841921_+|hypothetical-protein
MARRPVAWVVSLVLFAEALGVAALNWFLGVVVDRQNMSLAGLDPDMMSTSSKIGGVVFGLYFALCGLVALLVALRDRAPAGLGRVLLISAAVVHALLGAVTWGLVGWTAFLYMVVVFGLVVLLLMTYDGRPEQPADAAPGVSPGVPPGAAPGGGGAGGGAGGDGSPVTPPAPTTP
>CP023407.1|AYL37879.1|4841892_4842510_-|septum-formation-inhibitor-Maf
MTDQPRRRLVLASQSPARLGLLRQAGLDPEVIVSGVDEDAVTAPTPAELALALAEAKASVVAAKPEVKGALVIGCDSVLELDGQALGKPADAEEATARWKSMRGRAGTLQTGHCVYDTLSGRYVSATASTVVRFGEPTDEEVAAYVASGEPLHVAGAFTLDGRSAPFIDGIDGDHGNVIGISLPLVRRLLSGLGVGITELWAPGA
>CP023407.1|AYL37880.1|4842576_4842705_-|hypothetical-protein
MLWSDPDNEPPEDLRDTQDMLRRLGVLVALAMVFAMLVIGVR
>CP023407.1|AYL37881.1|4842812_4843022_-|hypothetical-protein
MTIKVVRGNPTPEELAAALAVVRARAAAASAEPSGAPAPRDAWSDPSRIAAHSVPRPGPVSWSRTYWPA
>CP023407.1|AYL37882.1|4843038_4844628_-|methylmalonyl-CoA-carboxyltransferase
MSEPEEQQPDIHTTAGKLADLQRRIEEATHAGSARAVEKQHAKGKLTARERIDLLLDEGSFVELDEFARHRSTNFGLDANRPYGDGVVTGYGTVDGRPVAVFSQDFTVFGGALGEVYGQKIVKVMDFALKTGCPVIGINDSGGARIQEGVASLGAYGEIFRRNTHASGVIPQISLVVGPCAGGAVYSPAITDFTIMVDQTSHMFITGPDVIKTVTGEDVGFEELGGARTHNTASGVAHHMAGDEKDAIEYVKQLLSYLPSNNLSEPPVFPEEADLTVTEEDRELDTIVPDSANQPYDMHAVIEHVLDDAEFFETQPLFAPNILTGYGRVEGHPVGIVANQPMQFAGCLDITASEKAARFVRTCDAFNIPVITFVDVPGFLPGVDQEHDGIIRRGAKLIFAYAEATVPLITVITRKAFGGAYDVMGSKHLGADLNLAWPTAQIAVMGAQGAVNILHRRTIAESDDPEEARARLMREYEDALLNPYVAAERGYVDAVIMPSDTRRHIVRGLRQLRTKRESLPPKKHGNIPL
>CP023407.1|AYL37883.1|4844738_4845608_+|biotin--[acetyl-CoA-carboxylase]-ligase
MTPRDASESPRNRWSDLDRPPLNVTALRRGLVREGSLWREVDVVQSTGSTNSDLVARAGGGREVAEGVVLVAEEQTAARGRLDRRWTAPARSGLFFSVLLRPAEVPVARWGWLPLLTGVAVATGLSRAAGVDTALKWPNDLLVTVGGEERKAGGILAERAGDDGVVVGVGINVSLRADELPVPTAGSLALAGAVSTDRDPLLRGVLRALEDWYGRWRGAGGDAGACGLQETYAAGCATLGRVVRAELPGDRELVGEAVAVDGDGRLVLATGEGVQEPVGAGDIVHLRGR
>CP023407.1|AYL37884.1|4846376_4847483_+|adenylate/guanylate-cyclase-domain-containing-protein
MTVDDSGSTDAQGAGEDPLALRLEQLILGAERRYTPFQAARSAGVTMELATRFWRAMGFADVGQAKALTEADVLALRRLAGLVEAGLLSEAMAVQVARSTGQTTARLAEWQIDSFLEGLTEPPEPGMTRTEVTYPIVELLLPELEEFLVYVWRRQLAASAGRVVQGADDEEMVDRRLAVAFADLVGFTRLTRRMEEEELGELVEAFETTAADLVAARGGRLIKTLGDEVLYAADDAGTAAEIAMLLIETMANDETMPELRVGMAFGTVTTRMGDVFGTTVNLASRLTSIAPRDAVLVDSAFAEELIRNGDAPASESAAAEAVAAAEKEGEEPPGYRFALQPMWQRPVRGLGVVEPWLLNRRNGASSAP
>CP023407.1|AYL37885.1|4848280_4849081_+|enoyl-CoA-hydratase
MSEQRFGEFVLVRRHERGHVAELVLDRPKAMNAVSTGMARSLAGACAALGEDRSVRAVVLTSSHERAFCVGADLKERNSFTDADLMRQRPVARGAYTGVLELPVPTVAAVHGFALGGGFELALSCDVIVADRTAVMGLPEVSVGVIPGGGGTQLLPRRVGAARAAELIFTARRVEAAEARELGLVDELVEQGKDREEALAMASRIAAHSPVGLRAAKRALRLGHGLDLAAGLEVEDAAWRSVAFSGDRAEGVAAFNEKRRPEWPGE
>CP023407.1|AYL39627.1|4849185_4850304_+|diguanylate-cyclase
MAAAHSSRGAWRAAARGACRALGGTFAALSVWERELGRLRVLVNVGELAEGEEEFPEDESYPVYQFAEITEFLHERWAGGGEPDAWVETAEGPPPGRAGYRHQRVAALRRRGRGCCVVAPIVLHGRAWGELYVARPVGAPVFGRGDADFATVLAAVVAAGIAQAERLEEARRLAYTDALTGLANRRAVDARLDEAVECHRRDGAVVSLVVCDLNGLKRVNDTQGHAVGDRLLERFGMVLSLCGAMLPGALAARLGGDEFCLLAVGPSADEVVKAADEVCRRAVELGIGDGVACGVASTEDPVGPVRSARRLFRLADAAQYRAKAERAEHPVVAGREGPGDPVVRLADEPSRAADGERRRFRGRHSPERPEPG

Crispr_ID: CP023407_8

• CRISPR_ID: CP023407_8
• CRISPR_location: 5220030-5220135
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 1 spacers

Consensus repeat of CP023407_8

Consensus_repeat	Method
GCCCGCGGAGTGCGGCTGCGGCGG	CRISPRCasFinder

spacers of CP023407_8

>8.1|5220054|58|CP023407|CRISPRCasFinder
CGCGGCTGCCCGCAGGGACGCGGGTCGCTGCGGGGACGCGGGTGGGGCGGCGCGGCGG

CRISPR arrays and Neighbor proteins around CP023407_8

CRISPR arrays

The CRISPR arrays of CP023407_8

>merge|CP023407|8|5220030-5220135|CRISPRCasFinder
GCCCGCGGAGTGCGGCTGCGGCGGCGCGGCTGCCCGCAGGGACGCGGGTCGCTGCGGGGACGCGGGTGGGGCGGCGCGGCGGCCCGCGGGAGTGCGGCTGCGGCGG

>CP023407|8|7|5220030-5220135|CRISPRCasFinder
GCCCGCGGAGTGCGGCTGCGGCGG	CGCGGCTGCCCGCAGGGACGCGGGTCGCTGCGGGGACGCGGGTGGGGCGGCGCGGCGG
CCCGCGGGAGTGCGGCTGCGGCGG

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
CP023407.1|AYL38181.1|5217032_5217242_+|HTH-domain-containing-protein	unknown	unknown	gnl|CDD|234647
CP023407.1|AYL38188.1|5225687_5226428_-|DNA-binding-response-regulator	unknown	unknown	gnl|CDD|223816
CP023407.1|AYL38194.1|5232666_5235951_+|histidine-kinase	unknown	unknown	gnl|CDD|377989
CP023407.1|AYL39657.1|5211264_5212017_-|amino-acid-ABC-transporter-ATP-binding-protein	unknown	unknown	gnl|CDD|224051
CP023407.1|AYL38180.1|5215963_5216929_+|Zn-dependent-oxidoreductase	unknown	unknown	gnl|CDD|176227
CP023407.1|AYL38179.1|5214561_5215800_+|NAD-dependent-malic-enzyme	unknown	unknown	gnl|CDD|223358
CP023407.1|AYL38186.1|5220483_5224287_-|multifunctional-oxoglutarate-decarboxylase/oxoglutarate-dehydrogenase-thiamine-pyrophosphate-binding-subunit/dihydrolipoyllysine-residue-succinyltransferase-subunit	unknown	unknown	gnl|CDD|237030
CP023407.1|AYL38177.1|5212037_5212976_-|ABC-transporter-permease	unknown	unknown	gnl|CDD|223836
CP023407.1|AYL38189.1|5226552_5227245_-|spermidine-synthase	unknown	unknown	gnl|CDD|223498
CP023407.1|AYL38191.1|5229904_5230729_-|lysozyme	unknown	unknown	gnl|CDD|119374
CP023407.1|AYL38185.1|5220223_5220403_-|hypothetical-protein	unknown	unknown	unknown
CP023407.1|AYL38178.1|5213014_5213977_-|atrA-protein	unknown	unknown	gnl|CDD|270225
CP023407.1|AYL38193.1|5231462_5232257_-|hypothetical-protein	unknown	unknown	unknown
CP023407.1|AYL38187.1|5224578_5225691_-|sensor-histidine-kinase	unknown	unknown	gnl|CDD|223715
CP023407.1|AYL38184.1|5218911_5219784_-|hypothetical-protein	unknown	unknown	gnl|CDD|372574
CP023407.1|AYL38176.1|5210414_5211185_+|class-I-SAM-dependent-methyltransferase	unknown	unknown	gnl|CDD|379312
CP023407.1|AYL38192.1|5230957_5231452_+|MarR-family-transcriptional-regulator	unknown	unknown	gnl|CDD|197670
CP023407.1|AYL38190.1|5227397_5229821_+|endopeptidase-La	unknown	unknown	gnl|CDD|223542
CP023407.1|AYL38183.1|5217866_5218910_-|PadR-family-transcriptional-regulator	unknown	unknown	gnl|CDD|367559
CP023407.1|AYL38182.1|5217241_5217805_+|peptidase	unknown	unknown	gnl|CDD|214361

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
CP023407.1|AYL38181.1|5217032_5217242_+|HTH-domain-containing-protein	gnl|CDD|234647	PRK00118, PRK00118, putative DNA-binding protein; Validated.	0.000411767
CP023407.1|AYL38188.1|5225687_5226428_-|DNA-binding-response-regulator	gnl|CDD|223816	COG0745, OmpR, Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain [Signal transduction mechanisms / Transcription].	5.73453e-82
CP023407.1|AYL38194.1|5232666_5235951_+|histidine-kinase	gnl|CDD|377989	pfam08376, NIT, Nitrate and nitrite sensing. The nitrate- and nitrite sensing domain (NIT) is found in receptor components of signal transducing pathways in bacteria which control gene expression, cellular motility and enzyme activity in response to nitrate and nitrite concentrations. The NIT domain is predicted to be all alpha-helical in structure.	1.6223e-20
CP023407.1|AYL39657.1|5211264_5212017_-|amino-acid-ABC-transporter-ATP-binding-protein	gnl|CDD|224051	COG1126, GlnQ, ABC-type polar amino acid transport system, ATPase component [Amino acid transport and metabolism].	8.07263e-155
CP023407.1|AYL38180.1|5215963_5216929_+|Zn-dependent-oxidoreductase	gnl|CDD|176227	cd08266, Zn_ADH_like1, Alcohol dehydrogenases of the MDR family. This group contains proteins related to the zinc-dependent alcohol dehydrogenases. However, while the group has structural zinc site characteristic of these enzymes, it lacks the consensus site for a catalytic zinc. NAD(P)(H)-dependent oxidoreductases are the major enzymes in the interconversion of alcohols and aldehydes, or ketones. Alcohol dehydrogenase in the liver converts ethanol and NAD+ to acetaldehyde and NADH, while in yeast and some other microorganisms ADH catalyzes the conversion acetaldehyde to ethanol in alcoholic fermentation. ADH is a member of the medium chain alcohol dehydrogenase family (MDR), which has a NAD(P)(H)-binding domain in a Rossmann fold of a beta-alpha form. The NAD(H)-binding region is comprised of 2 structurally similar halves, each of which contacts a mononucleotide. A GxGxxG motif after the first mononucleotide contact half allows the close contact of the coenzyme with the ADH backbone. The N-terminal catalytic domain has a distant homology to GroES. These proteins typically form dimers (typically higher plants, mammals) or tetramers (yeast, bacteria), and have 2 tightly bound zinc atoms per subunit, a catalytic zinc at the active site, and a structural zinc in a lobe of the catalytic domain. NAD(H)-binding occurs in the cleft between the catalytic and coenzyme-binding domains at the active site, and coenzyme binding induces a conformational closing of this cleft. Coenzyme binding typically precedes and contributes to substrate binding. In human ADH catalysis, the zinc ion helps coordinate the alcohol, followed by deprotonation of a histidine, the ribose of NAD, a serine, then the alcohol, which allows the transfer of a hydride to NAD+, creating NADH and a zinc-bound aldehyde or ketone. In yeast and some bacteria, the active site zinc binds an aldehyde, polarizing it, and leading to the reverse reaction.	1.04327e-102
CP023407.1|AYL38179.1|5214561_5215800_+|NAD-dependent-malic-enzyme	gnl|CDD|223358	COG0281, SfcA, Malic enzyme [Energy production and conversion].	2.36826e-176
CP023407.1|AYL38186.1|5220483_5224287_-|multifunctional-oxoglutarate-decarboxylase/oxoglutarate-dehydrogenase-thiamine-pyrophosphate-binding-subunit/dihydrolipoyllysine-residue-succinyltransferase-subunit	gnl|CDD|237030	PRK12270, kgd, multifunctional oxoglutarate decarboxylase/oxoglutarate dehydrogenase thiamine pyrophosphate-binding subunit/dihydrolipoyllysine-residue succinyltransferase subunit.	0
CP023407.1|AYL38177.1|5212037_5212976_-|ABC-transporter-permease	gnl|CDD|223836	COG0765, HisM, ABC-type amino acid transport system, permease component [Amino acid transport and metabolism].	1.39683e-80
CP023407.1|AYL38189.1|5226552_5227245_-|spermidine-synthase	gnl|CDD|223498	COG0421, SpeE, Spermidine synthase [Amino acid transport and metabolism].	4.42456e-18
CP023407.1|AYL38191.1|5229904_5230729_-|lysozyme	gnl|CDD|119374	cd06412, GH25_CH-type, CH-type (Chalaropsis-type) lysozymes represent one of four functionally-defined classes of peptidoglycan hydrolases (also referred to as endo-N-acetylmuramidases) that cleave bacterial cell wall peptidoglycans. CH-type lysozymes exhibit both lysozyme (acetylmuramidase) and diacetylmuramidase activity. The first member of this family to be described was a muramidase from the fungus Chalaropsis. However, a majority of the CH-type lysozymes are found in bacteriophages and Gram-positive bacteria such as Streptomyces and Clostridium. CH-type lysozymes have a single glycosyl hydrolase family 25 (GH25) domain with an unusual beta/alpha-barrel fold in which the last strand of the barrel is antiparallel to strands beta7 and beta1. Most CH-type lysozymes appear to lack the cell wall-binding domain found in other GH25 muramidases.	6.9774e-111
CP023407.1|AYL38178.1|5213014_5213977_-|atrA-protein	gnl|CDD|270225	cd01004, PBP2_MidA_like, Mimosine binding domain of ABC-type transporter MidA and similar proteins; the type 2 periplasmic binding protein fold. This subgroup includes the periplasmic binding component of ABC transporter involved in uptake of mimosine MidA and its similar proteins. This periplasmic binding domain belongs to the type 2 periplasmic-binding fold protein (PBP2) superfamily, whose members are involved in chemotaxis and uptake of nutrients and other small molecules from the extracellular space as a primary receptor. PBP2 typically comprises of two globular subdomains connected by a flexible hinge and bind their ligand in the cleft between these domains in a manner resembling a Venus flytrap. After binding their specific ligand with high affinity, they can interact with a cognate membrane transport complex comprised of two integral membrane domains and two receptor cytoplasmically-located ATPase domains. This interaction triggers the ligand translocation across the cytoplasmic membrane energized by ATP hydrolysis.	7.95943e-78
CP023407.1|AYL38187.1|5224578_5225691_-|sensor-histidine-kinase	gnl|CDD|223715	COG0642, BaeS, Signal transduction histidine kinase [Signal transduction mechanisms].	9.26242e-52
CP023407.1|AYL38184.1|5218911_5219784_-|hypothetical-protein	gnl|CDD|372574	pfam13349, DUF4097, Putative adhesin. This has a putative all-beta structure with a twenty-residue repeat with a highly conserved repeating GD, gly-asp, motif. It may form part of a bacterial adhesin.	1.19983e-07
CP023407.1|AYL38176.1|5210414_5211185_+|class-I-SAM-dependent-methyltransferase	gnl|CDD|379312	pfam13649, Methyltransf_25, Methyltransferase domain. This family appears to be a methyltransferase domain.	1.47119e-17
CP023407.1|AYL38192.1|5230957_5231452_+|MarR-family-transcriptional-regulator	gnl|CDD|197670	smart00347, HTH_MARR, helix_turn_helix multiple antibiotic resistance protein.	1.68279e-19
CP023407.1|AYL38190.1|5227397_5229821_+|endopeptidase-La	gnl|CDD|223542	COG0466, Lon, ATP-dependent Lon protease, bacterial type [Posttranslational modification, protein turnover, chaperones].	0
CP023407.1|AYL38183.1|5217866_5218910_-|PadR-family-transcriptional-regulator	gnl|CDD|367559	pfam03551, PadR, Transcriptional regulator PadR-like family. Members of this family are transcriptional regulators that appear to be related to the pfam01047 family. This family includes PadR, a protein that is involved in negative regulation of phenolic acid metabolism.	2.30702e-21
CP023407.1|AYL38182.1|5217241_5217805_+|peptidase	gnl|CDD|214361	CHL00095, clpC, Clp protease ATP binding subunit.	2.5678e-06

Protein sequences

>CP023407.1|AYL38184.1|5218911_5219784_-|hypothetical-protein
MPESSVAEPTKLTFDAPVTELHVRIVNGTVNVVGTDEGPARLEVSEIEGPPLRVTEKNGTLTVAYDDLPWKGFLKWLDRKGWRRSAVVSLAVPARTRVEVGVVGAGAMVSGIRGPAAVKGVAGDTTLVALSGPVRADTVTGNVEAQSVTGDLRFASVSGDLTVVDGSGSSVRADSVSGSMIVDLDPDGPTDVRLTSVSGEIAIRLPEPADADVEANTASGAISNAFDDLRIEGQWGAHKLTGRLGAGSGKLRATTVSGSIALLRRPAGEDEPGPRDGSGETAGTTDKKVL
>CP023407.1|AYL38183.1|5217866_5218910_-|PadR-family-transcriptional-regulator
MPPVFAHGRLRLYLLKLLDEAPRHGYEVIRLLEERFQGLYAPSAGTVYPRLAKLEAEGLVTHTTEGGRKVYSITDAGRAELADRSGELADLELEIRESVAELAAEIRADVSGAAGDLRREMRAAAGQARQSRKGGASSEEGGGFGDFDFGVFGDSDAWRAAKEEMRRAKQEWKEQARRAKDESRRSREEAQRARRQAKEAQDRAQEQVQRIARQVQEQVQGHFARGDWPTGLREGLGEVVKEMSEFGKDFAFGRPGAGGPGKTSEPSYSRTSEDFPAEYVPAWGHGDPSDSTGDPARDLERLLDRFRDDVRDAARDHGVTADQYRDARRHLSTAAAHIGAILRAPKS
>CP023407.1|AYL38182.1|5217241_5217805_+|peptidase
MFERFTKDARDVVKGAVEQAEGAGAGRVGAEHLLLALLDREGSRASFALSALGAGERRESVRTALGEARRRAGLTQAETDALAGIGIDVSEIVARVEEVHGTGAMSGDRKGPGRRSGHRPFDRAAKDVLTGALRAATALRDRRIGDEHLLLALTTRPGVPAEVLADHGVTYESVTRVLYGEGEAKAG
>CP023407.1|AYL38181.1|5217032_5217242_+|HTH-domain-containing-protein
MTEATDLAERAGDRDPRVGLRAVAALRRLLEQLEAVQVRNARNQGWSWQEIAAELGVSRQAVHKKYGRH
>CP023407.1|AYL38180.1|5215963_5216929_+|Zn-dependent-oxidoreductase
MFAVYAARIDRDQPLTGLELGERPAPESRPGWSTVTVKAASLNHHDLWSLRGVGLAEDKLPMILGCDAAGIDEDGNEVVLHSVIGQSGHGVGPKEPRSILTERYQGTFAEQVAVPTWNILPKPKELSFEEAACLPTAWLTAYRMLFTNAGVRPGDSVLVQGAGGGVATAAIVLGRAAGLRVFATSRDEAKRKRALELGAVEAVEPGARLPQRVDAVIETVGAATWSHSVKSLRPGGTLVISGATSGDRPSHAELTRIFFLELKIVGSTMGTKDELEDLLSFCAAAGVRPVIDEALPMDRAREGFERMESGGQFGKIVLTNP
>CP023407.1|AYL38179.1|5214561_5215800_+|NAD-dependent-malic-enzyme
MAAEIVNPRSDSESRNGPTERENGAEPLDSFDPAFALHRGGKMAVQATVPIRDKDDLSLAYTPGVAKVCSAIAEQPDLVHDYTWKSSVVAVVTDGTAVLGLGDIGPEASLPVMEGKAILFKQFGGVDAVPLALNCTDVDEIVETVVRLAPSFGGVNLEDISAPRCFEIERRLQERLDIPVFHDDQHGTAVVSLAALRNAARLSGRTLGELRAVISGAGAAGVAIARMLVEAGIGDVAVADRKGVVSRDRDDLTPIKAELASFTNKAGISGSLEAALAGADVFIGVSGGTVPEPAVASMAEGAFVFAMANPDPEVHPEVAHKYAAVVATGRSDFPNQINNVLAFPGIFAGALQVRASRITEGMKLAAAEALASVVGDDLAADYVIPSPFDERVAPAVTAAVAAAARAEGVARR
>CP023407.1|AYL38178.1|5213014_5213977_-|atrA-protein
MTASTTRRTTAARSRLAAAGAIAVAGALLLTGCGDQTKDKGSGESKSESKAPLADKLPQSIRDKGVVKVGSDIAYAPVEFKDDSGKVVGIDPDLAAAMGKQLGVTFEFENATFDTLIGGLASKRYDMAMSAMTDTKDRQEGVDADTGKKVGAGVDFVDYFTAGVSLYTNKGADQGIKTWDDLCGKTMAVQRNTFSHDLAKDQAKKCTDAGEKTLKIEPFDTNPEAETRMRSKGADVVSADFPIAAYSVKNSGGGKYFEIVGDQVEAGPYGIAVAKDNTELRDALQAAVQAVIDSGEYQKIVDKWGVADGAITEAKINGGS
>CP023407.1|AYL38177.1|5212037_5212976_-|ABC-transporter-permease
MTADVNKTDGPGATPSAGPEAIKAIPVRHPGRYVSAVVALALLGAVVYAFAQGKINWGAIPDYFFDDRILHGVGNTLLLTVLSMVIGIAGGILLAVMRLSKNPVTSSLAWFYIWFFRGTPVLVQLFVWFNLGLVFEYINLGPIYKDYWASFMTPLLTALLGLGLNEAAYMSEICRAGLLSVDEGQTEASHALGMSHTKTLRRIVVPQAMRVIVPPTGNEVINMLKTTSLVSAVQFADLFQEAKDIGQNSGSPVEMYFLAAAWYLIMTSILSVGQYYIERHYARGSVRSLPPTPLQRFRAAVLPTRRTKGVPA
>CP023407.1|AYL39657.1|5211264_5212017_-|amino-acid-ABC-transporter-ATP-binding-protein
MVKSEGVHKSFGAVEVLKGIDLEVQSGEVFCLIGPSGSGKSTFLRCINHLEKINAGRLYVDGELVGYRQKGDKLYELKDSEVALKRRDIGMVFQRFNLFPHMTAVENVMEAPVQVKGVSKAQARERAMQLLDRVGLGDKTGNYPSQLSGGQQQRVAIARALAMEPKLMLFDEPTSALDPELVGDVLDVMRDLAESGMTMVVVTHEMGFAREVGDSLVFMDGGVVVESGNPRDVLTDPQHERTKAFLSKVL
>CP023407.1|AYL38176.1|5210414_5211185_+|class-I-SAM-dependent-methyltransferase
MTDADTTTATTDWGAWQQSWDRQQEWYMPDREERFRIMLDMVEALVGTAPRVLDLACGTGSITARLLDRFPDATSTGVDLDPALLAIAEGTFAGDGRVTFVTADLKDPDWPAALPHDSYDAVLTATALHWLHREPLADLYGHVAGLVRDGGVFMNADHMIDETTPGINAAERALRHARMDRARRDGALDWAEWWQLAAKDPVLAGPTARRFAIYGEHADGDMPGAGWHAAVLREKGFAEARPVWCSPSDTLLLALK
>CP023407.1|AYL38185.1|5220223_5220403_-|hypothetical-protein
MYFTDRGIEELEKRRGEEEVTFEWLAEQLRTFVDLNPDFEVPVERLATWLARLDDEEDD
>CP023407.1|AYL38186.1|5220483_5224287_-|multifunctional-oxoglutarate-decarboxylase/oxoglutarate-dehydrogenase-thiamine-pyrophosphate-binding-subunit/dihydrolipoyllysine-residue-succinyltransferase-subunit
MSPQSPSNSSISTDTDQAGKNPAAAFGANEWLVDEIYQQYLQDPNSVDRAWWDFFADYKPGAPATSAPAGTAAAGATGTTGTAQPAAPARPAAPAPAPAAAQPAQAAPAAAPAAKAPAQAPAPAKAAAPAAKPAAAKAEPAAEAKTGPEQVTLRGPSAAVAKNMDASLELPTATSVRAVPVKLLFDNRIVINNHLKRARGGKISFTHLIGYAMVQAIKAMPTMNHSFARVDGKPTLVKPEHVNLGLAIDLVKPNGDRQLVVAAIKKAETLNFFEFWQAYEDIVRRARDNKLTMDDFTGVTVSLTNPGGLGTVHSVPRLMPGQAVIMGVGSMDYPAEFQGTSQDTLNKLGISKVMTLTSTYDHRVIQGAASGEFLRAVANLLLGEKGFYDEIFEALRIPYEPVRWLKDIDASHDDDVTKAARVFELIHSYRVRGHVMADTDPLEYRQRKHPDLDIVEHGLTLWDLEREFAVGGFAGKSLMKLRDILGVLRDSYCRTTGVEFMHIQDPKQRKWIQDRIERSHTKPEREEQLRILRRLNAAEAFETFLQTKYVGQKRFSLEGGESVIPLLDAVLDSAAESRLDEVVIGMAHRGRLNVLANIVGKSYAQIFREFEGNLDPKSMHGSGDVKYHLGAEGTFTGLDGEQIKVSLVANPSHLEAVDPVLEGVSRAKQDIIGKGGTDFTVLPVAIHGDAAFAGQGVVAETLNMSQLRGYRTGGTVHVVINNQVGFTAAPESSRSSMYATDVARMIEAPIFHVNGDDPEAVVRVARLAFEFRQAFNKDVVIDLICYRRRGHNESDNPAFTQPLMYDLIDKKRSVRKLYTESLIGRGDITLEEAEQALQDYQGQLEKVFTEVREATSQPAAVEPSDPQAEFPVAVNTAVTTETVKRIAESQVNIPDHITVHPRLLPQLQRRASMVEDGTIDWGMGETLALGSLLLEGVPVRLAGQDSQRGTFGQRHAVIIDRSTGEEYTPLQYLSEDQGRLNVYNSLLSEYAAMGFEYGYSLARPDALVLWEAQFGDFVNGAQTVVDEFISSAEQKWAQTSGVVLLLPHGYEGQGPDHSSARPERFLQLCAQNNMTVAMPTSPSNYFHLLRWQAHNPHHKPLVVFTPKSMLRLKAAAAKAEEFTTGQFRPVIGDASVDPAAVKKVVFTAGKVYYDLEAERQKRGATDIAIIRIERLYPLPGAELQAEIKKYPNAEKYLWAQEEPANQGAWPFIALNLIDHLDLAVGADVPHDERLRRISRPHSSSPAVGSAKRHQAEQEQLVREVFEA
>CP023407.1|AYL38187.1|5224578_5225691_-|sensor-histidine-kinase
MSDGPAGRRGPGAQPPWGGVRPFSIQTKLGALVVVSVLITTGLSVIAVHTKTELRFITIFSMIATLLITQFVAHSLTAPLDDMNAVARSISHGDYTRRVRENRRDELGGLAQTINAMADELEAQDRQRKELVANVSHELRTPIAGLRAVLENIVDGVTRADPETMRTALKQTERLGRLVETLLDLSRLDNGVVPLKKRRFEVWPYLSGVLKEANMVASARAGMASGSGSHTRTDVHLHLDVSPPELVAHADPERIHQVVANLIDNAVKHSPPHGRVTVRARRGERPESLELEVLDEGPGIPRTEWHRVFERFNRGNVNRPHGPGSDGGTGLGLAIARWAVDLHGGRIGVAESERGCRILVTLPGASSVPS
>CP023407.1|AYL38188.1|5225687_5226428_-|DNA-binding-response-regulator
MEQTQTSHNGTATTTPGAQRRVLVVEDDATIVDAIATRLRAEGFLVQTAGDGPSAVDTAEAWQPDLLILDIMLPGFDGLEVCRRVQAQRPLPVLMLTARDDETDMLVGLGVGADDYMTKPFSMRELAARVHVLLRRMERAALAATTPRSGILRLGELEIDHAQRRVRVRSEDVHLTPTEFDLLVCLANTPRAVLSREQLLAEVWDWADASGTRTVDSHIKALRRKIGAERIRTVHGVGYALETPTP
>CP023407.1|AYL38189.1|5226552_5227245_-|spermidine-synthase
MSTPYDIPEAPEILDRREGPYGEVVLRRHGDLLQIIANGCFLMDTSDGRSERRLVDAALAALDGRTAHRPHVLIGGLGVGFSLAHAAGQPEWGRVAVVEREPAIIEWHRGGPLAALSARALADPRTEIIETDLVRYVHETSDTFDALCLDIDNGPDWTVTEDNGTLYSLSGLAACERVLRPGGVLAVWSAQPSPEFEGTLRNAGFRQVRTEEVPVARGVPDVVHIGVRPG
>CP023407.1|AYL38190.1|5227397_5229821_+|endopeptidase-La
MAAESTPLALPVLPLDDEVVLPGMVVPLDLSDSEVRAAVEAAQAAARSAPGKPRVLLVPRVDGTYAGMGVLGTVEQVGRLADGDPGALIRGRGRVRIGAGTTGPGAALWVEGTRTDDSVPEPLPGHVSELVKEYKALATAWLRKRGAWQVVDRVQAIDDVSALADNSGYSPFLTTEQKVALLETTDPVARLKLATQQLRDHLAEQDVAETIAKDVQEGVDKQQREFLLRRQLEAVRKELRELNGEGGKDSAEESDDYRARVEAADLPEKVREAALKEVDKLERSSDQSPEGSWIRTWLDTVLEMPWNERTEDAYDIRGARAVLDAEHAGLEDVKERITEYLAVRKRRSDRGLGVVGGRRGGAVLALVGPPGVGKTSLGESVAHAMGRKFVRVALGGVRDEAEIRGHRRTYVGALPGRIVRAVKEAGSMNPVVLLDEIDKVGSDFRGDPAAALLEVLDPAQNHTFRDHYLEVELDLSDVVFLATANVLEAIPEALLDRMELVRLDGYTEDEKVVIARDHLLPRQLERAGLAAEEVTVDEGALRKLAGEYTREAGVRTLERSLARLLRKVAARHELGERELPFTVTAADLRDLIGRPHHVPESAQDPAERRTAVPGVATGLAVTGAGGDVLYVEASLADPETGAAGLTLTGQLGDVMKESAQIALSFLRSHGAELELPVGDLKDRGAHIHFPAGAVPKDGPSAGVTMTTALASLLSGRLVRTDVAMTGEVSLTGRVLPIGGVKQKLLAAHRAGITTVIIPKRNEPDLDDVPAEVLDGLDVHAVTDVRQVLELALSPATSGAAPEVPVAA
>CP023407.1|AYL38191.1|5229904_5230729_-|lysozyme
MPVHSSRLTRIRALGTAGAVLLAVLSLPPAAVAAESAPTTPPRGSAHMGMGVVGHDGQDGLPTARSTQTEGVDVSSHQGNVAWNTLWNSGVRWAYAKATEGTYYSNPYHSQQYDGSYDVGMIRGAYHFGTPDTASGAAQADYFVNRGGGWTADGRTLPGVLDIEWNPYGDACYGKSASGMVSWIRDFLDQYKARTGRNAVIYTATSWWKQCTGNYSGFAATTPLWIARYASTAGELPAGWSTYTMWQYTSTGPTVGDHDRFNGTLDRVRALALG
>CP023407.1|AYL38192.1|5230957_5231452_+|MarR-family-transcriptional-regulator
MHEDGNGGAEHAATPEPARGVNQPGFLALERELTVLLRRARASQGEMARAVHPDLEPSAYGLLVRLDECGGQRATELAAYIGVGKATMSRQLRALEELGLVAREPDPADGRAWLVAVTREGRERVSRVREARRARYAGRLADWDPAEVTELARLLNQLNRGTEK
>CP023407.1|AYL38193.1|5231462_5232257_-|hypothetical-protein
MRTELVSEPGGPGRPNEDFASVGLPASGLGGSLVVLDGVTPPRDGGGCLHSVPWFTARLGGALTELTVSLPDVPLPELLSRALIRTADSHAATCDLSHPRTPQATVVLARWSPASVEYLVLSDSALLAGSPDGSVTALLDDRLDRLPRSALATHALVDTTLRNKEGGFFTAAADPSVASRAVAGSLPREEVRTLTALTDGAARWVERFHQGDWADCLALVREEGARSLVDRVRDLERADSGLVQPRAGKTHDDATVVHVELSPR
>CP023407.1|AYL38194.1|5232666_5235951_+|histidine-kinase
MQKSRPRRTGKQTAPAGDAEQAPTVGRGRPTHVRNRLIVAVAVVAAAIAAAGAPSVLAASGDLSDSQELVTLAEQTQDAVTLAHSLADERDEVVPYIAAGRSKSKAPTEEHSARVDRQVEELRTDTDLPEELRDGLDAVAAVRRAALTGKSSALEAHQAYSTAITRLHRLADQLAELTPPRAGSGAHALTELDSAVQQAAATRGLLLAALNVPSNTQTVIDPITGLPSTTTGSSDADTKRRDALTAAALQARLRSDAALADFRDTAPKDARASYDSTVTGPEVDTAEKYLAALTDEPALADDELDTDVKKLRAALTARVETMRGVESALFDKRTKDLAQLRDDDVTALEIRVAALGALMLLAIGVATAMARTLTRPLSVLRRGSARLAEAEDPTTQEPVAFTGRNDEFAQVVRSVNALHAHAAALTARIGTLEADRKHLVGQRQKMADAREELRTELAESAAHLERLRSGIGGTFVNLALRTLGLVERQLGVIEGLEEREDDPDRLATLFKLDHFATVMRRHSENLLVLAGTEHGRNTAGPVPLVDVVRAAVSEIERYERVRIAALPPHAHIAGFAADDLSHLLAELMENATASSPPDVPVEVSGWLLENGEVMLSVQDEGIGMTGERLERLNSRLSGFDPETTYDIEGEDGLGLGLYVVARLAHRHGVRVKLREQKQGGIAAVVVLPDPLLTAAAPGAVPASVATPAYSLPGADAEANSNVLHGRAESDDPLVALAEKAVRHQGLPDTGTPTDTPADDSAPARAGEPTPGRADAAAPVRADEPAATPAETSARAHAGDPAAGLEDDADGSEVSADASGPDRAAPVTADEPTVSSASRAGTSGPGLADDADVTEPAPADEPAATPAEQSERASGEGVAAEGGDERGTGDAGRRDVPSERPAEMTMELLIPVQPAAPEAARPGAGHADVRDTGPADAAEQPSGVGADEAVRPGAGHTAEDEAAPGAGHPAEDEAAPPTPAGGSPLPRRPVETAGRDGGTEHARAADGDEEPVTAKGLPKRTPKITAPAAAPRRRAGAVDADALRRRLGGFRRGADAGRRDVEAELAERTGDHRTPSGPDAATEETTGGTVEEASS

Crispr_ID: CP023407_9

• CRISPR_ID: CP023407_9
• CRISPR_location: 6207220-6207310
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 1 spacers

Consensus repeat of CP023407_9

Consensus_repeat	Method
AGCCACGAACCACCCGCACCCGGCA	CRISPRCasFinder

spacers of CP023407_9

>9.1|6207245|41|CP023407|CRISPRCasFinder
GACGGCGGTATGCCTCGAGGGGCGCGGGGAACTGCGCGACC

CRISPR arrays and Neighbor proteins around CP023407_9

CRISPR arrays

The CRISPR arrays of CP023407_9

>merge|CP023407|9|6207220-6207310|CRISPRCasFinder
AGCCACGAACCACCCGCACCCGGCAGACGGCGGTATGCCTCGAGGGGCGCGGGGAACTGCGCGACCAGCCACGAACCACCCGCACCCGGCA

>CP023407|9|8|6207220-6207310|CRISPRCasFinder
AGCCACGAACCACCCGCACCCGGCA	GACGGCGGTATGCCTCGAGGGGCGCGGGGAACTGCGCGACC
AGCCACGAACCACCCGCACCCGGCA

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
CP023407.1|AYL38882.1|6201434_6202040_-|polyisoprenoid-binding-protein	unknown	unknown	gnl|CDD|377274
CP023407.1|AYL38890.1|6213238_6213634_-|DUF742-domain-containing-protein	unknown	unknown	gnl|CDD|283084
CP023407.1|AYL38892.1|6214168_6217975_-|hypothetical-protein	unknown	unknown	gnl|CDD|377989
CP023407.1|AYL38888.1|6209028_6212292_-|ATPase	unknown	unknown	gnl|CDD|377989
CP023407.1|AYL38887.1|6207937_6208510_-|DUF742-domain-containing-protein	unknown	unknown	gnl|CDD|283084
CP023407.1|AYL38894.1|6218929_6219715_+|2-hydroxyhepta-2,4-diene-1,7-dioate-isomerase	unknown	unknown	gnl|CDD|223257
CP023407.1|AYL38877.1|6195335_6195467_+|hypothetical-protein	unknown	unknown	unknown
CP023407.1|AYL38880.1|6198264_6199869_+|citramalate-synthase	unknown	unknown	gnl|CDD|237068
CP023407.1|AYL38891.1|6213745_6214159_-|dynein-regulation-protein-LC7	unknown	unknown	gnl|CDD|367422
CP023407.1|AYL38879.1|6196826_6197915_+|branched-chain-amino-acid-aminotransferase	unknown	unknown	gnl|CDD|237363
CP023407.1|AYL39725.1|6208604_6209018_-|dynein-regulation-protein-LC7	unknown	unknown	gnl|CDD|367422
CP023407.1|AYL38884.1|6205229_6206813_+|methylmalonyl-CoA-carboxyltransferase	unknown	unknown	gnl|CDD|130187
CP023407.1|AYL38889.1|6212667_6213258_-|ATP-binding-protein	unknown	unknown	gnl|CDD|225138
CP023407.1|AYL38893.1|6218595_6218769_+|hypothetical-protein	unknown	unknown	unknown
CP023407.1|AYL38885.1|6206848_6207037_+|acyl-CoA-carboxylase-subunit-epsilon	unknown	unknown	gnl|CDD|379386
CP023407.1|AYL38886.1|6207375_6207957_-|ATP-binding-protein	unknown	unknown	gnl|CDD|225138
CP023407.1|AYL38878.1|6195534_6196578_+|3-isopropylmalate-dehydrogenase	unknown	unknown	gnl|CDD|179579
CP023407.1|AYL39724.1|6202224_6203724_-|hopanoid-C-3-methylase-HpnR	unknown	unknown	gnl|CDD|275160
CP023407.1|AYL38883.1|6204020_6204875_+|short-chain-dehydrogenase	unknown	unknown	gnl|CDD|223377
CP023407.1|AYL38881.1|6199964_6201314_+|hypothetical-protein	unknown	unknown	gnl|CDD|374433

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
CP023407.1|AYL38882.1|6201434_6202040_-|polyisoprenoid-binding-protein	gnl|CDD|377274	pfam04264, YceI, YceI-like domain. E. coli YceI is a base-induced periplasmic protein. The recent structure of a member of this family shows that it binds to poly-isoprenoid. The structure consists of an extended, eight-stranded, antiparallel beta-barrel that resembles the lipocalin fold.	7.65348e-57
CP023407.1|AYL38890.1|6213238_6213634_-|DUF742-domain-containing-protein	gnl|CDD|283084	pfam05331, DUF742, Protein of unknown function (DUF742). This family consists of several uncharacterized Streptomyces proteins as well as one from Mycobacterium tuberculosis. The function of these proteins is unknown.	6.18694e-40
CP023407.1|AYL38892.1|6214168_6217975_-|hypothetical-protein	gnl|CDD|377989	pfam08376, NIT, Nitrate and nitrite sensing. The nitrate- and nitrite sensing domain (NIT) is found in receptor components of signal transducing pathways in bacteria which control gene expression, cellular motility and enzyme activity in response to nitrate and nitrite concentrations. The NIT domain is predicted to be all alpha-helical in structure.	4.81281e-34
CP023407.1|AYL38888.1|6209028_6212292_-|ATPase	gnl|CDD|377989	pfam08376, NIT, Nitrate and nitrite sensing. The nitrate- and nitrite sensing domain (NIT) is found in receptor components of signal transducing pathways in bacteria which control gene expression, cellular motility and enzyme activity in response to nitrate and nitrite concentrations. The NIT domain is predicted to be all alpha-helical in structure.	2.82747e-27
CP023407.1|AYL38887.1|6207937_6208510_-|DUF742-domain-containing-protein	gnl|CDD|283084	pfam05331, DUF742, Protein of unknown function (DUF742). This family consists of several uncharacterized Streptomyces proteins as well as one from Mycobacterium tuberculosis. The function of these proteins is unknown.	1.15043e-44
CP023407.1|AYL38894.1|6218929_6219715_+|2-hydroxyhepta-2,4-diene-1,7-dioate-isomerase	gnl|CDD|223257	COG0179, MhpD, 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) [Secondary metabolites biosynthesis, transport, and catabolism].	3.28123e-101
CP023407.1|AYL38880.1|6198264_6199869_+|citramalate-synthase	gnl|CDD|237068	PRK12344, PRK12344, putative alpha-isopropylmalate/homocitrate synthase family transferase; Provisional.	0
CP023407.1|AYL38891.1|6213745_6214159_-|dynein-regulation-protein-LC7	gnl|CDD|367422	pfam03259, Robl_LC7, Roadblock/LC7 domain. This family includes proteins that are about 100 amino acids long and have been shown to be related. Members of this family of proteins are associated with both flagellar outer arm dynein and Drosophila and rat brain cytoplasmic dynein. It is proposed that roadblock/LC7 family members may modulate specific dynein functions. This family also includes Golgi-associated MP1 adapter protein and MglB from Myxococcus xanthus, a protein involved in gliding motility. However the family also includes members from non-motile bacteria such as Streptomyces coelicolor, suggesting that the protein may play a structural or regulatory role.	5.94463e-23
CP023407.1|AYL38879.1|6196826_6197915_+|branched-chain-amino-acid-aminotransferase	gnl|CDD|237363	PRK13357, PRK13357, branched-chain amino acid aminotransferase; Provisional.	0
CP023407.1|AYL39725.1|6208604_6209018_-|dynein-regulation-protein-LC7	gnl|CDD|367422	pfam03259, Robl_LC7, Roadblock/LC7 domain. This family includes proteins that are about 100 amino acids long and have been shown to be related. Members of this family of proteins are associated with both flagellar outer arm dynein and Drosophila and rat brain cytoplasmic dynein. It is proposed that roadblock/LC7 family members may modulate specific dynein functions. This family also includes Golgi-associated MP1 adapter protein and MglB from Myxococcus xanthus, a protein involved in gliding motility. However the family also includes members from non-motile bacteria such as Streptomyces coelicolor, suggesting that the protein may play a structural or regulatory role.	1.51599e-22
CP023407.1|AYL38884.1|6205229_6206813_+|methylmalonyl-CoA-carboxyltransferase	gnl|CDD|130187	TIGR01117, methylmalonyl-CoA_decarboxylase_alpha-subunit, methylmalonyl-CoA decarboxylase alpha subunit. This model describes methymalonyl-CoA decarboxylase aplha subunit in archaea and bacteria. Metylmalonyl-CoA decarboxylase Na+ pump is a representative of a class of Na+ transport decarboxylases that couples the energy derived by decarboxylation of carboxylic acid substrates to drive the extrusion of Na+ ion across the membrane. [Energy metabolism, ATP-proton motive force interconversion, Energy metabolism, Fermentation, Transport and binding proteins, Cations and iron carrying compounds].	0
CP023407.1|AYL38889.1|6212667_6213258_-|ATP-binding-protein	gnl|CDD|225138	COG2229, COG2229, Predicted GTPase [General function prediction only].	8.12365e-46
CP023407.1|AYL38885.1|6206848_6207037_+|acyl-CoA-carboxylase-subunit-epsilon	gnl|CDD|379386	pfam13822, ACC_epsilon, Acyl-CoA carboxylase epsilon subunit. This family includes the epsilon subunits of propionyl-CoA carboxylase, EC:6.4.1.3, and acetyl-CoA carboxylase, EC:6.4.1.2. These enzymes are involved in the biosynthesis of long-chain fatty acids. The epsilon subunit is necessary for an efficient interaction between the alpha and beta subunits of these enzymes.	1.93825e-08
CP023407.1|AYL38886.1|6207375_6207957_-|ATP-binding-protein	gnl|CDD|225138	COG2229, COG2229, Predicted GTPase [General function prediction only].	1.87223e-45
CP023407.1|AYL38878.1|6195534_6196578_+|3-isopropylmalate-dehydrogenase	gnl|CDD|179579	PRK03437, PRK03437, 3-isopropylmalate dehydrogenase; Provisional.	0
CP023407.1|AYL39724.1|6202224_6203724_-|hopanoid-C-3-methylase-HpnR	gnl|CDD|275160	TIGR04367, protein_of_the_SAM_superfamily, hopanoid C-3 methylase HpnR. Members of this are family are a B12-binding domain/radical SAM domain protein required for 3-methylhopanoid production. Activity was confirmed by mutant phenotype by disrupting this gene in Methylococcus capsulatus strain Bath. This protein family should only occur in genomes that encode a squalene-hopene cyclase (see TIGR01507). [Fatty acid and phospholipid metabolism, Biosynthesis].	0
CP023407.1|AYL38883.1|6204020_6204875_+|short-chain-dehydrogenase	gnl|CDD|223377	COG0300, DltE, Short-chain dehydrogenases of various substrate specificities [General function prediction only].	1.13564e-58
CP023407.1|AYL38881.1|6199964_6201314_+|hypothetical-protein	gnl|CDD|374433	pfam16208, Keratin_2_head, Keratin type II head.	0.003236

Protein sequences

>CP023407.1|AYL38885.1|6206848_6207037_+|acyl-CoA-carboxylase-subunit-epsilon
MSTPDIRVEKGHAEPEEVAAITAILLARAAAQPSAPAHRGRPRAGWRRLEREPGFRAPHSWR
>CP023407.1|AYL38884.1|6205229_6206813_+|methylmalonyl-CoA-carboxyltransferase
MTVLDEAPGEPTDARGRVAELHEIRAQALAGPSEKATKAQHAKGKLTARERIELLLDPGSFREVEQLRRHRATGFGLEEKKPFTDGVITGWGTVEGRTVFVYAHDFRIFGGALGEAHATKIHKIMDMAIAAGAPLVSLNDGAGARIQEGVSALAGYGGIFQRNTKASGVIPQISVMLGPCAGGAAYSPALTDFVFMVRDTSQMFITGPDVVKAVTGEEITQNGLGGADVHAETSGVCHFAYDDEETCIHEVRYLLSLLPQNNRENPPRVECSDDADRRGDVLLDLVPADGNRPYDMAKVIEEIVDDGEYLEVHERWARNIICALARMDGQVVGIVANQPQSLAGVLDIEASEKAARFVQMCDAFNIPILTFLDVPGFLPGVDQEHGGIIRHGAKLLYAYCNATVPRISLILRKAYGGAYIVMDSQSIGADLTYAWPTNEIAVMGADGAANVIFRRQIAEAEDPEAMRARMVKEYKSELMHPYYAAERGLVDDVIDPAETREVLIRSLAMLQTKHADLPSRKHGNPPQ
>CP023407.1|AYL38883.1|6204020_6204875_+|short-chain-dehydrogenase
MTTTGSGSTYDPPADPAHGAAPAPTPAAARGHGPPRTDRRVVLVTGASSGIGAATARRFAAGGWHLLLSGRDRRRLEETASGTSAVLLPADLAAPEGAKMLAEAALRKAGRIDVLVAGAGIGWAGPFLGMPHTDIDRVLFLDLNAALHLVREVLPSMVAAGRGRVVLVGSVAGSVGVREEAVYSAAKAGLAAFAEALRQELRGTGVGVTLVVPGPVETGFFARRGRPYHRSHPRPTSPGRVAGAVWDAVSHGRDDTYVPAWLTLPARVRALTPGLFRRLLNRFG
>CP023407.1|AYL39724.1|6202224_6203724_-|hopanoid-C-3-methylase-HpnR
MRLLLVHPSALMYSEIFLRLEPLGLERVAGAARAAGHEVRVVDLQVLGVNRLRDEVRSFRPEALGVSLNYLANIPEAIALAARVKREVPGCFVFFGGHSVSFVAEEVIDQAAGAVDAVVRGEGETAVGPLLEAVRDGGVEGVPGVVTAAGRGPAPLMLHSIDAPLPARDLMRNRRRYFIGELDPCASVEFTRGCPWDCSFCSAWTFYGRSYRKASPEAAAEDLARIREPNVFIVDDVAFIRPEHGNAIADEVERRGIRKRYYLETRADVLLRHPEVFERWARLGLRYMFLGMEAIDAEGLDLYRKRVSPDENLKALETARRLGIQVAINLIVDPAWDEERFRVVREFALAVPEIVHFTVMTPYPGTEIWHTESRRLTTRDYRLFDIQHAVVPTTLPLERFYEELVRTQAVINRKHLGLRTAFGAARVLGRNLLHGQTNFARMLWKFNQVYNPRRQLADHARAVRYELPLPQRLDVGDRRRLYVHTRDATKGAPTGGLPD
>CP023407.1|AYL38882.1|6201434_6202040_-|polyisoprenoid-binding-protein
MGIFGRKSNETAATTAAAVNPDLAALTGDYTIDPAHTTLGFVARHAMVTNVKGTFQDFTGTLHLDGGDPSKSTATIDVVMDSIETGNADRDGHLKSADFFKTDEFPAMTFRTTKAEALGGDDYRITGDLTILGTTKQLSIDLEFNGAATDPFGNQRVGFEGKAEILRSEWGLTWNAALETGGVLVSDKIKLNFDISAIRNA
>CP023407.1|AYL38881.1|6199964_6201314_+|hypothetical-protein
MKVARTRTFVRLLIVPVAAALAVLTAGAPGAQAATDVSEVARQLRESPVYVDPGAQDILAKSDAQALADKIEAADKPVFVTVLPADYPTQNLFQNLRTETGVTGLYGIRLGDRFDARADSSVLSRQGVRNLVTSVQGAGDAKAQLNDFVDSALRNTGGTAPGSWSDGASSGVPVGGLITVGAVLVAGGAGGYALVRRNRRRHEEERRVALEKLRVVVDEDITAFGEELDRLDFHPGEPGADDAMRADYEHALDAYEQSKSFMAAARKPEDVRAVTESVEEGRFALASLAARREGRPVPERRPPCFFDPRHGPSVADAVWTPPGGAEREVPVCAADRARLADGRDPAVREVDTEYGRRPYWDAGPAYGPWAGGYFGGGLLPGLLVGTMLGGMMAGPAYAADYGAGYGDFGGYQGGDVSGADFNGGDFGGDFGGGFGGGDFGGGGDFGGGF
>CP023407.1|AYL38880.1|6198264_6199869_+|citramalate-synthase
MTATSEPDDSFHVFDTTLRDGAQREGINLTVADKLAIARHLDEFGVGFIEGGWPGANPRDTEFFARAQQEIDFRHAQLVAFGATRRAGTTADQDPQVRALLDSGAQVITLVAKSHDRHVELALRTTLDENLEMVRDTVSFLKGRGRRVFVDCEHFFDGYRANAEYAKAVVRAASGAGADVVVLCDTNGGMLPAQIQAVVATVLADTGARLGIHAQDDTGCAVANTLAAVDAGATHVQCTANGYGERVGNANLFPVVAALELKYGKQVLPEGRLREMTRISHAIAEVVNLTPSTHQPYVGVSAFAHKAGLHASAIKVDPDLYQHIDPEQVGNTMRMLVSDMAGRASIELKGKELGIDLGGDRELVGRVVERVKERELEGYTYEAADASFELLLRAEAEGRALKYFDVESWRAIAEDRPDGTHANEATVKLWAKGERIVATAEGNGPVNALDRALRVALEKIYPQLAKLELVDYKVRILEGKHGTHSTTRVLISTSDGAGEWSTVGVAENVIAASWQALEDAYAYGLLRAGVEPAA
>CP023407.1|AYL38879.1|6196826_6197915_+|branched-chain-amino-acid-aminotransferase
MTTPTIELKPSAHPLPHAEREAILANPGFGRHFTDHMVTIKWTEGRGWHDGQLVPYAPISLDPATTVLHYAQEIFEGLKAYRRPDGSVATFRPEKNAERFQRSARRLAMPELPVETFIEACDVLVAQDKAWVPAHGGEESLYLRPFMIATEVGLGVKPANEYLFLVIASPAGAYFPGGVKPVSIWVSEDRVRAVPGGMGDAKTGGNYAASLLAQAEAAAKGCDQVCYLDAVEHQWVEELGGMNLYFVYGDKIVTPALSGSILEGVTRDSLLGVARDLGYEAVEERVSVEQWQRDSENGSLTEVFACGTAAVITPVGTVKRAGGEWQQGGGEPGEVTVRLRQALLDIQRGKAEDKHGWMHPLG
>CP023407.1|AYL38878.1|6195534_6196578_+|3-isopropylmalate-dehydrogenase
MSRSLNLAVIPGDGIGQEVVAEGLKVLSAVLPQDVKLETKEYDFGARRYHATGETLTDADLDELKRHDAILLGAIGDPSVPSGILERGFLLKLRFAFDHHVNLRPSKLLPGVATPLAGEPQIDFVVVREGTEGPYTGNGGTIRKGTPHEVATEVSVNTAFGVERVVRDAFARAQARPRKKLTLVHKNNVLTFAGHLWTNVFNKVAEEYPEVTTDYIHVDAATIYLVTDPARFDVIVTDNLFGDIITDLAAAVSGGIGVAASGNINPGREFPSMFEPVHGSAPDIAGQGKADPSATVLSVALLLRHLGHDAEATRIEDAVSADLAERVGLPARSTSEIGDALAARVAG
>CP023407.1|AYL38877.1|6195335_6195467_+|hypothetical-protein
MAETTVRRRVRHASRTTESDRKNAAAALQRALDRRDNGGSTGH
>CP023407.1|AYL38886.1|6207375_6207957_-|ATP-binding-protein
MDFASSSGGPSRSTTSAKIVVAGGFGVGKTTFVGAVSEINPLRTEAVMTSASAGIDDLTHTGDKTTTTVAMDFGRITLDQDLILYLFGTPGQDRFWFMWDDLVRGAIGAIVLVDTRRLADCFPAVDYFENSGLPFVIALNGFDGQQPYQPDEVREALQIGPDTPIITTDARHRADAKSALITLVEHALMARLR
>CP023407.1|AYL38887.1|6207937_6208510_-|DUF742-domain-containing-protein
MATPPDGSSAGNWSYGPAQDHNDGSQNRYGYPSAPQHRQPYVPQGPGPSPYDQPYAPRIQPVEPQRRTPEPAPAGASNNPLVRPYAMTGGRTRPRYQLAIEALVHTTAQPHQMQGQLPEHQRICNLCREIKSVAEVSALLTIPLGVARILVADLAEAGLVAIHQPGGDENAGGQPDVTLLERVLSGLRKL
>CP023407.1|AYL39725.1|6208604_6209018_-|dynein-regulation-protein-LC7
MSQAAQNLNWLITNFVDNTPGVSHTVVVSADGLLLAMSEGFPRDRADQLAAVASGLTSLTAGASRIFEGGSVNQTVVEMERGFLFIMSISDGSSLAVLAHPEADIGLIGYEMALLVDRAGTVLTPDLRAELQGSLLN
>CP023407.1|AYL38888.1|6209028_6212292_-|ATPase
MRRSKNSPEPSARGNFTPPPRTAAPAPVPGPEPTAAPAPSGGRLSPRNWRVTTRLNAILLIPVLVGLVMGGFQVKSSIDTWNEAEDAENTARLVQASLTYANALYNERDVTAVPLLQGKGKSDPDVTKARAATDKAADAFDEAAQSMPGKPGLERRLRLFRETEPQLTALRAGAYTSKLKGVQTEEGYVAVAHPLMEFANELGLGTGNITSYGRSVYAISLTKAALSLQRSIGMHLLVKPGPTDSQLASQRVALTSYAYLEGIAIEEYKGAGTDADTAKLEQTAQRIEAEGAAMAKEAKQQDPDYVPPPAKPETMISELGRLPSTDAAARADLAGQGITPANWWAVNTLKYDGYREIESDLTDTAVNEASQIADDARRDAFVTGAVVVVALLAAFILAGMVARQMSRSMRQLRNAAFGIAEQRLPMLVDQLSRTDPGRVDTRVAPIPITSTDEIGEVARAFDQVHREAVRLAAEQALLRGNINAIFTNLSRRNQSLIEGQLTLITDLENNEADPDQLENLFRLDHLATRMRRNGENLLVLAGEEPGRRWDQPVPLVDVLRAASSEVEQYERIELSGVPEAEIHGRAVTDLVHLLAELLENATTFSSPQTKVRVTATRLPDGRVMIEIHDKGIGLTAEDFADINHKLANPPTVDAAISQRMGLFVVGRLSDRHGIRVQLRPSGEQAGTTSLVMLPDAITHGGGGEQHAQRDEFTVSQIIPEQNFGGEDFSRQQQPLRTAAELGFDDSRYTEVPDDIRDLDPVGRSLMREERRAALESQAQPELTAANAQENPEAPAFPDEFAAPQGYDNGQGFDGGYQGQQSAGYDQQAYDNGQQTPYAEPQQQAYGEGYYAPNGQVPQNDAFSSNGGYPEQSYADPARDGHAAAGATGSFTPFTERRQDDDWPQQDGYRNGYPDQYPAQAPEPELTQAADAPEQDSVGFDRPGPVPSDAHELTDAGLPRRGSTGGGGANGTGGARSVNQEPPASPQENDGDSWRSANDERWQQASSLRKPKAGGVTSSGLPRRVPKANLVQGAAETTPQGGPQVSRAPEDVRGRLSNLRRGVQRGRSAGSETNGQGFGSDSTYNQER
>CP023407.1|AYL38889.1|6212667_6213258_-|ATP-binding-protein
MDFASSSGGAVSENRATTSAKIVVAGGFGVGKTTFVGAVSEINPLRTEAVMTSASAGIDDLTHTGDKTTTTVAMDFGRITLDQDLILYLFGTPGQDRFWFMWDDLVRGAIGAVVLVDTRRLADCFPAVDYFENSGLPFVVALNGFDGQQPYQPEEVREALQIGPDTPIITTDARHRADAKSALITLVEHALMARLR
>CP023407.1|AYL38890.1|6213238_6213634_-|DUF742-domain-containing-protein
MTPPTASHDPYAEPYEDEGDQPLVRPYAMTGGRTRPRYQLAIEALISTTADPAALMGLLPEHQRICHLCREVKSVAEVSALLNMPLGVARILVADLAEAGLVAVHQPGGDETTGAPDVTLLERVLSGLRKL
>CP023407.1|AYL38891.1|6213745_6214159_-|dynein-regulation-protein-LC7
MSQAAQNLNWLITNFVDNTPGVSHTVVVSADGLLLAMSEGFPRDRADQLAAVASGLTSLTAGASRIFEGGSVAQTVVEMERGFLFLMSVSDGSSLAVLAHPECDIGLVGYEMALLVDRAGAVLTPDLRAELQGSLLH
>CP023407.1|AYL38892.1|6214168_6217975_-|hypothetical-protein
MQGRFKRDGSASAEPEPHGGTGPKAVGSSPQHAQNQGPASSGNAGGATTGGPAAPAPAAPKGPTGPGPRIALRNWRISTRLVSLLALPVVAATSLGALRINQSMDDIQQLDNMKLLTDMTKQATELAAALQEERDQSAGPLAHGGKATDFSVKGFREKTDRARNVFIDSSEEIDEASKDGNLKGVRDNLVQLAGDLGELEKIRNTAFKSERNSTQTVEGYHRLIVNLIDLSQDMAEATSNPDMIQRTRALASFSSAKEYASIQRAVLAAALPASNTSKGDLSENDRLYAESALASQKSELRSFKSIYGDVTASELLLPIEEGNSTIKATDEYAGRALASEFGLASVGKRSYRDWLDDSSTKIQQMKNIELTLLEEMEQKARELRSATERDAIVSGVVILIVLGVSLVGAFVVARSMIRSLRRLEETATKVAQERLPDLVKQLSESDPQDVDTSVESVGVHSRDEIGRVAAAFDDVHREAVRLAAEQALLRGNVNAMFTNLSRRSQGLIQRQLSLISELESREADPDQLSSLFKLDHLATRMRRNGENLLVLAGEEPGRRWTRPVPLVDVLRAAASEVEQYERIELASVPSTEVAGRVVNDLVHLLAELLENATSFSSPQTKVKVTGHALPDGRVLIEIHDTGIGLSPEDLAAINERLASPPTVDVSVSRRMGLFVVGRLSQRHGIRIQLRPSDSGGTTALVMLPVDVAQGGKKPQGKPGQPGAGGGGPAAAQAAAGAAAARRAAGAGQPGGGALGSAPSGGRLGAGQGPRAALPGRDGSGRPDGPRTPQGQPGNAAPSLFDRGAPTASPQGRPAAPAGAGFGGQAPGAPQGMQSPAQGGQQDRDAFGGARGQVPPQRGGGNTERGRRPQLPPRGGARPELPGGEQPRVPSWSDENAQPPVPRASLDTPRGHEEPDTARTSAMPRFDDRQGPGATAEIPRFDERHNPGAPGYGAPGADVPQNTGQFPQHTGQYAQPGANGSQDPGQFVRGDIFGAPQGRPNAQNPAATGQFPAPQGYDNGSTGQFQMPGQDNGSTGRHSLPERPNAGDPAATGQFELPQANGTQGGGFPAPRPPAPQARPVHPEQVNGGAARRPDERPASDAWALPPASGPGDGRTPLYDTLETNWFRGGGAGGAPEQGNGSAPAPQQPQQQPQSPQSQQPQGPTGPQRSASTAWRSSPNDELVRQAERVRQPAAGGVTTSGLPRRVPKANLVPGTAQQQQHQSGPQVSRAPDDVRGRLTNLRRGIAQGRQAGTTQTGSFPNPTHQQER
>CP023407.1|AYL38893.1|6218595_6218769_+|hypothetical-protein
MFHKIANPRRTTLAHLDGAGELQTPAQQEHAVELPAQTANPRRTILMEIPVAADAAK
>CP023407.1|AYL38894.1|6218929_6219715_+|2-hydroxyhepta-2,4-diene-1,7-dioate-isomerase
MRIARFSIDGNVAFGAVEGEKQDELVLDIIKGIPFADFELSGTKVPLSKVRLLPPVLPNKVVAFGRNYADHAKEMGNEVPDAPFAFFKPATSVIGPGDEIQYPSFSQEVHHEAELAVVIGRLCREVPRDRVKDVIFGYTCANDITARDVQKREKQWARAKGFDTSCPLGPWVETDLDIAAANDLTIQLTVNGAQRQLGRTSEMTHPIEDLIVNISEAMTLLPGDVILTGTPAGVGPLNVGDEVAVTIEGIGTLTNKVVKRG

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity
CP023407_1	1.3|1700843|30|CP023407|CRT	1700843-1700872	30	CP023407.1	1701092-1701121	0	1.0
CP023407_1	1.4|1700891|39|CP023407|CRT	1700891-1700929	39	CP023407.1	1701140-1701178	0	1.0
CP023407_1	1.5|1700948|18|CP023407|CRT	1700948-1700965	18	CP023407.1	3643687-3643704	0	1.0
CP023407_1	1.5|1700948|18|CP023407|CRT	1700948-1700965	18	CP023407.1	3643798-3643815	0	1.0
CP023407_1	1.5|1700948|18|CP023407|CRT	1700948-1700965	18	CP023407.1	186488-186505	1	0.944
CP023407_1	1.5|1700948|18|CP023407|CRT	1700948-1700965	18	CP023407.1	1701185-1701202	1	0.944
CP023407_1	1.5|1700948|18|CP023407|CRT	1700948-1700965	18	CP023408.1	433856-433873	1	0.944
CP023407_1	1.7|1701053|21|CP023407|CRT	1701053-1701073	21	CP023407.1	1700510-1700530	1	0.952
CP023407_1	1.2|1700804|21|CP023407|CRT	1700804-1700824	21	CP023407.1	1700561-1700581	2	0.905
CP023407_1	1.5|1700948|18|CP023407|CRT	1700948-1700965	18	CP023408.1	445566-445583	2	0.889

1. spacer 1.3|1700843|30|CP023407|CRT matches to position: 1701092-1701121, mismatch: 0, identity: 1.0

aaaccaccacggtcgtcacgacggaaaccg	CRISPR spacer
aaaccaccacggtcgtcacgacggaaaccg	Protospacer
******************************

2. spacer 1.4|1700891|39|CP023407|CRT matches to position: 1701140-1701178, mismatch: 0, identity: 1.0

cggtcgtcacggccacggaagccaccgcgatcgtcgccc	CRISPR spacer
cggtcgtcacggccacggaagccaccgcgatcgtcgccc	Protospacer
***************************************

3. spacer 1.5|1700948|18|CP023407|CRT matches to position: 3643687-3643704, mismatch: 0, identity: 1.0

tcgtcacggcggtcgtca	CRISPR spacer
tcgtcacggcggtcgtca	Protospacer
******************

4. spacer 1.5|1700948|18|CP023407|CRT matches to position: 3643798-3643815, mismatch: 0, identity: 1.0

tcgtcacggcggtcgtca	CRISPR spacer
tcgtcacggcggtcgtca	Protospacer
******************

5. spacer 1.5|1700948|18|CP023407|CRT matches to position: 186488-186505, mismatch: 1, identity: 0.944

tcgtcacggcggtcgtca	CRISPR spacer
tcgtcacggcggtcatca	Protospacer
**************.***

6. spacer 1.5|1700948|18|CP023407|CRT matches to position: 1701185-1701202, mismatch: 1, identity: 0.944

tcgtcacggcggtcgtca	CRISPR spacer
tcgtcacgacggtcgtca	Protospacer
********.*********

7. spacer 1.5|1700948|18|CP023407|CRT matches to position: 433856-433873, mismatch: 1, identity: 0.944

tcgtcacggcggtcgtca	CRISPR spacer
tcgtcacggcggtcggca	Protospacer
*************** **

8. spacer 1.7|1701053|21|CP023407|CRT matches to position: 1700510-1700530, mismatch: 1, identity: 0.952

aaaccaccacggtccccaccg	CRISPR spacer
aagccaccacggtccccaccg	Protospacer
**.******************

9. spacer 1.2|1700804|21|CP023407|CRT matches to position: 1700561-1700581, mismatch: 2, identity: 0.905

aagccaccgcggtcaccgctg	CRISPR spacer
aagccgcctcggtcaccgctg	Protospacer
*****.** ************

10. spacer 1.5|1700948|18|CP023407|CRT matches to position: 445566-445583, mismatch: 2, identity: 0.889

tcgtcacggcggtcgtca	CRISPR spacer
tcgccacggcggccgtca	Protospacer
***.********.*****

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity
CP023407_1	1.2|1700804|21|CP023407|CRT	1700804-1700824	21	NZ_CP016083	Streptomyces sp. SAT1 plasmid unnamed3, complete sequence	125996-126016	2	0.905
CP023407_1	1.1|1700756|30|CP023407|CRT	1700756-1700785	30	NZ_CP032322	Azospirillum brasilense strain MTCC4035 plasmid p1, complete sequence	104351-104380	5	0.833
CP023407_1	1.1|1700756|30|CP023407|CRT	1700756-1700785	30	NZ_LC066384	Aureimonas sp. AU12 plasmid pAU12rrn, complete sequence	3743-3772	6	0.8
CP023407_1	1.1|1700756|30|CP023407|CRT	1700756-1700785	30	NZ_CP024310	Sinorhizobium fredii strain NXT3 plasmid pSfreNXT3c, complete sequence	848621-848650	6	0.8
CP023407_1	1.1|1700756|30|CP023407|CRT	1700756-1700785	30	NZ_CP023064	Sinorhizobium sp. CCBAU 05631 plasmid pSS05631b, complete sequence	668111-668140	6	0.8
CP023407_1	1.1|1700756|30|CP023407|CRT	1700756-1700785	30	NZ_CP027299	Streptomyces sp. SGAir0924 plasmid unnamed_5	350827-350856	6	0.8
CP023407_1	1.1|1700756|30|CP023407|CRT	1700756-1700785	30	NZ_CP020911	Rhizobium etli strain NXC12 plasmid pRetNXC12e, complete sequence	120097-120126	6	0.8
CP023407_1	1.1|1700756|30|CP023407|CRT	1700756-1700785	30	MH229865	Streptomyces phage LazerLemon, complete genome	37329-37358	6	0.8
CP023407_1	1.1|1700756|30|CP023407|CRT	1700756-1700785	30	NC_014030	Salinibacter ruber M8 plasmid pSR61, complete sequence	47119-47148	7	0.767
CP023407_1	1.1|1700756|30|CP023407|CRT	1700756-1700785	30	NC_007765	Rhizobium etli CFN 42 plasmid p42e, complete sequence	414574-414603	7	0.767
CP023407_1	1.1|1700756|30|CP023407|CRT	1700756-1700785	30	NZ_CP020909	Rhizobium etli strain NXC12 plasmid pRetNXC12c, complete sequence	415029-415058	7	0.767
CP023407_1	1.1|1700756|30|CP023407|CRT	1700756-1700785	30	NC_021908	Rhizobium etli bv. mimosae str. Mim1 plasmid pRetMIM1d, complete sequence	421065-421094	7	0.767
CP023407_1	1.1|1700756|30|CP023407|CRT	1700756-1700785	30	NZ_CP032340	Azospirillum brasilense strain MTCC4038 plasmid p1, complete sequence	1497663-1497692	7	0.767
CP023407_1	1.1|1700756|30|CP023407|CRT	1700756-1700785	30	NC_007678	Salinibacter ruber DSM 13855 plasmid pSR35, complete sequence	7115-7144	7	0.767
CP023407_1	1.1|1700756|30|CP023407|CRT	1700756-1700785	30	KT997858	Uncultured Mediterranean phage uvDeep-CGR2-KM19-C37, complete genome	12705-12734	7	0.767
CP023407_1	1.1|1700756|30|CP023407|CRT	1700756-1700785	30	MN234219	Mycobacterium phage Mercurio, complete genome	25367-25396	7	0.767
CP023407_1	1.1|1700756|30|CP023407|CRT	1700756-1700785	30	MN234185	Mycobacterium phage Lemuria, complete genome	24763-24792	7	0.767
CP023407_1	1.1|1700756|30|CP023407|CRT	1700756-1700785	30	CP042595	Streptomyces albogriseolus strain LBX-2 plasmid pSALBX2, complete sequence	234903-234932	7	0.767
CP023407_1	1.1|1700756|30|CP023407|CRT	1700756-1700785	30	NZ_CP032346	Azospirillum brasilense strain MTCC4039 plasmid p1, complete sequence	95568-95597	7	0.767
CP023407_1	1.1|1700756|30|CP023407|CRT	1700756-1700785	30	NZ_CP012915	Azospirillum brasilense strain Sp 7 plasmid ABSP7_p1, complete sequence	634708-634737	7	0.767
CP023407_1	1.1|1700756|30|CP023407|CRT	1700756-1700785	30	NZ_KX443401	Rhodococcus hoagii strain PAM1572 plasmid pVAPN1572, complete sequence	89882-89911	8	0.733
CP023407_1	1.1|1700756|30|CP023407|CRT	1700756-1700785	30	NZ_CP013379	Burkholderia pseudomultivorans strain SUB-INT23-BP2 plasmid pINT23, complete sequence	9792-9821	8	0.733
CP023407_1	1.1|1700756|30|CP023407|CRT	1700756-1700785	30	NC_021289	Burkholderia insecticola plasmid p1, complete sequence	110175-110204	8	0.733
CP023407_1	1.3|1700843|30|CP023407|CRT	1700843-1700872	30	NZ_CP018075	Streptomyces venezuelae strain NRRL B-65442 plasmid, complete sequence	133613-133642	8	0.733
CP023407_1	1.3|1700843|30|CP023407|CRT	1700843-1700872	30	MT939492	Xanthomonas phage Xoo-sp14, complete genome	92436-92465	8	0.733
CP023407_1	1.1|1700756|30|CP023407|CRT	1700756-1700785	30	NZ_CP032347	Azospirillum brasilense strain MTCC4039 plasmid p2, complete sequence	597882-597911	9	0.7
CP023407_1	1.1|1700756|30|CP023407|CRT	1700756-1700785	30	NC_049830	Escherichia phage PGN590, complete genome	15290-15319	9	0.7

1. spacer 1.2|1700804|21|CP023407|CRT matches to NZ_CP016083 (Streptomyces sp. SAT1 plasmid unnamed3, complete sequence) position: , mismatch: 2, identity: 0.905

aagccaccgcggtcaccgctg	CRISPR spacer
ccgccaccgcggtcaccgctg	Protospacer
  *******************

2. spacer 1.1|1700756|30|CP023407|CRT matches to NZ_CP032322 (Azospirillum brasilense strain MTCC4035 plasmid p1, complete sequence) position: , mismatch: 5, identity: 0.833

tcacgccggaagccgccgcggtc--accaccg	CRISPR spacer
tcacgccggaagccgcggcgggcggaccgc--	Protospacer
**************** **** *  ***.*  

3. spacer 1.1|1700756|30|CP023407|CRT matches to NZ_LC066384 (Aureimonas sp. AU12 plasmid pAU12rrn, complete sequence) position: , mismatch: 6, identity: 0.8

tcacgccggaagccgccgcggtcaccaccg	CRISPR spacer
gcacgctggaagccgccgcggtcaccgtgc	Protospacer
 *****.*******************..  

4. spacer 1.1|1700756|30|CP023407|CRT matches to NZ_CP024310 (Sinorhizobium fredii strain NXT3 plasmid pSfreNXT3c, complete sequence) position: , mismatch: 6, identity: 0.8

tcacgccggaagccgccgcggtcaccaccg	CRISPR spacer
aatcgccggcaaccgccgcggtcaccacct	Protospacer
   ****** *.***************** 

5. spacer 1.1|1700756|30|CP023407|CRT matches to NZ_CP023064 (Sinorhizobium sp. CCBAU 05631 plasmid pSS05631b, complete sequence) position: , mismatch: 6, identity: 0.8

tcacgccggaagccgccgcggtcaccaccg	CRISPR spacer
aatcgccggcaaccgccgcggtcaccacct	Protospacer
   ****** *.***************** 

6. spacer 1.1|1700756|30|CP023407|CRT matches to NZ_CP027299 (Streptomyces sp. SGAir0924 plasmid unnamed_5) position: , mismatch: 6, identity: 0.8

tcacgccggaagccgccgcggtcaccaccg	CRISPR spacer
ccagaccgggagccgccgcgatcaccacgg	Protospacer
.** .****.**********.******* *

7. spacer 1.1|1700756|30|CP023407|CRT matches to NZ_CP020911 (Rhizobium etli strain NXC12 plasmid pRetNXC12e, complete sequence) position: , mismatch: 6, identity: 0.8

tcacgccggaagccgccgcggtcaccaccg	CRISPR spacer
acaaaccggaagccgcggcggtcaccctcg	Protospacer
 ** .*********** ********* .**

8. spacer 1.1|1700756|30|CP023407|CRT matches to MH229865 (Streptomyces phage LazerLemon, complete genome) position: , mismatch: 6, identity: 0.8

tcacgccggaagccgccgcggtcaccaccg	CRISPR spacer
tcacgccggaagcccgcgcggtcctgaacg	Protospacer
**************  ******* . * **

9. spacer 1.1|1700756|30|CP023407|CRT matches to NC_014030 (Salinibacter ruber M8 plasmid pSR61, complete sequence) position: , mismatch: 7, identity: 0.767

tcacgccggaagccgccgcggtcaccaccg	CRISPR spacer
tgtccccggaggccgccgcggtcacgacgt	Protospacer
*  * *****.************** **  

10. spacer 1.1|1700756|30|CP023407|CRT matches to NC_007765 (Rhizobium etli CFN 42 plasmid p42e, complete sequence) position: , mismatch: 7, identity: 0.767

tcacgccggaagccgccgcggtcaccaccg	CRISPR spacer
tcacgccggtagccgccgcggcaaagctcg	Protospacer
********* ***********. *   .**

11. spacer 1.1|1700756|30|CP023407|CRT matches to NZ_CP020909 (Rhizobium etli strain NXC12 plasmid pRetNXC12c, complete sequence) position: , mismatch: 7, identity: 0.767

tcacgccggaagccgccgcggtcaccaccg	CRISPR spacer
tcacgccggtagccgccgcggcaaagctcg	Protospacer
********* ***********. *   .**

12. spacer 1.1|1700756|30|CP023407|CRT matches to NC_021908 (Rhizobium etli bv. mimosae str. Mim1 plasmid pRetMIM1d, complete sequence) position: , mismatch: 7, identity: 0.767

tcacgccggaagccgccgcggtcaccaccg	CRISPR spacer
tcacgccggtagccgccgcggcaaagctcg	Protospacer
********* ***********. *   .**

13. spacer 1.1|1700756|30|CP023407|CRT matches to NZ_CP032340 (Azospirillum brasilense strain MTCC4038 plasmid p1, complete sequence) position: , mismatch: 7, identity: 0.767

tcacgccggaagccgccgcggtc--accaccg	CRISPR spacer
tcacgccggaagccgcggcgggtgggccgc--	Protospacer
**************** **** .  .**.*  

14. spacer 1.1|1700756|30|CP023407|CRT matches to NC_007678 (Salinibacter ruber DSM 13855 plasmid pSR35, complete sequence) position: , mismatch: 7, identity: 0.767

tcacgccggaagccgccgcggtcaccaccg	CRISPR spacer
tgtccccggaggccgccgcggtcacgacgt	Protospacer
*  * *****.************** **  

15. spacer 1.1|1700756|30|CP023407|CRT matches to KT997858 (Uncultured Mediterranean phage uvDeep-CGR2-KM19-C37, complete genome) position: , mismatch: 7, identity: 0.767

tcacgccggaagccgccgcggtcaccaccg	CRISPR spacer
acctgacggaagccgaggcggtcaccacct	Protospacer
 * .* *********  ************ 

16. spacer 1.1|1700756|30|CP023407|CRT matches to MN234219 (Mycobacterium phage Mercurio, complete genome) position: , mismatch: 7, identity: 0.767

tcacgccggaagccgccgcggtc-----accaccg	CRISPR spacer
tcacgacggacgccgccgcggtccagagac-----	Protospacer
***** **** ************     **     

17. spacer 1.1|1700756|30|CP023407|CRT matches to MN234185 (Mycobacterium phage Lemuria, complete genome) position: , mismatch: 7, identity: 0.767

tcacgccggaagccgccgcggtcaccaccg	CRISPR spacer
tcacgacggacgccgccgcggtccacaggc	Protospacer
***** **** ************  **   

18. spacer 1.1|1700756|30|CP023407|CRT matches to CP042595 (Streptomyces albogriseolus strain LBX-2 plasmid pSALBX2, complete sequence) position: , mismatch: 7, identity: 0.767

tcacgccggaagccgccgcggtcaccaccg	CRISPR spacer
cccagcgtgaagccgccgcggtcaccgccc	Protospacer
.*  **  ******************.** 

19. spacer 1.1|1700756|30|CP023407|CRT matches to NZ_CP032346 (Azospirillum brasilense strain MTCC4039 plasmid p1, complete sequence) position: , mismatch: 7, identity: 0.767

tcacgccggaagccgccgcggtcaccaccg	CRISPR spacer
tcacgccggaagccgctgcgggcggcccgc	Protospacer
****************.**** *. * *  

20. spacer 1.1|1700756|30|CP023407|CRT matches to NZ_CP012915 (Azospirillum brasilense strain Sp 7 plasmid ABSP7_p1, complete sequence) position: , mismatch: 7, identity: 0.767

tcacgccggaagccgccgcggtc--accaccg	CRISPR spacer
tcacgccggaagccgcggcgggtgggccgc--	Protospacer
**************** **** .  .**.*  

21. spacer 1.1|1700756|30|CP023407|CRT matches to NZ_KX443401 (Rhodococcus hoagii strain PAM1572 plasmid pVAPN1572, complete sequence) position: , mismatch: 8, identity: 0.733

tcacgccggaagccgccgcggtcaccaccg	CRISPR spacer
tgatcgcggcagccgccgcggtcaccatgc	Protospacer
* *.  *** *****************.  

22. spacer 1.1|1700756|30|CP023407|CRT matches to NZ_CP013379 (Burkholderia pseudomultivorans strain SUB-INT23-BP2 plasmid pINT23, complete sequence) position: , mismatch: 8, identity: 0.733

tcacgccggaagccgccgcggtcaccaccg	CRISPR spacer
gcacgccgaaagccgccgcggtatggcgcg	Protospacer
 *******.*************      **

23. spacer 1.1|1700756|30|CP023407|CRT matches to NC_021289 (Burkholderia insecticola plasmid p1, complete sequence) position: , mismatch: 8, identity: 0.733

tcacgccggaagccgccgcggtcaccaccg	CRISPR spacer
tcacgccggaagccgccacgctcgatgaag	Protospacer
*****************.** **. ..  *

24. spacer 1.3|1700843|30|CP023407|CRT matches to NZ_CP018075 (Streptomyces venezuelae strain NRRL B-65442 plasmid, complete sequence) position: , mismatch: 8, identity: 0.733

--aaaccaccacggtcgtcacgacggaaaccg	CRISPR spacer
ccggacgg--gcggtcgtcacggcggaaaccg	Protospacer
  ..** .  .***********.*********

25. spacer 1.3|1700843|30|CP023407|CRT matches to MT939492 (Xanthomonas phage Xoo-sp14, complete genome) position: , mismatch: 8, identity: 0.733

aaaccaccacggtcgtcacgacggaaaccg	CRISPR spacer
ccaccacgacggtcgtcacgacgatcatca	Protospacer
  ***** ***************.  *.*.

26. spacer 1.1|1700756|30|CP023407|CRT matches to NZ_CP032347 (Azospirillum brasilense strain MTCC4039 plasmid p2, complete sequence) position: , mismatch: 9, identity: 0.7

tcacgccggaagccgccgcggtcaccaccg	CRISPR spacer
aagcgccggcggccgccgcggtcaccgtgc	Protospacer
  .****** .***************..  

27. spacer 1.1|1700756|30|CP023407|CRT matches to NC_049830 (Escherichia phage PGN590, complete genome) position: , mismatch: 9, identity: 0.7

tcacgccggaagccgccgcggtcaccaccg	CRISPR spacer
caacgccgaaagccgcagcggtcactcttt	Protospacer
. ******.******* ********. .. 

2. CP023408