CRISPRimmunity

Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Self targeting spacer number	Target MGE spacer number	Prophage number	Anti-CRISPR protein number
CP024736	Akkermansia muciniphila strain AMDK-1 chromosome, complete genome	2 crisprs	cas4,DEDDh,cas3,cas9,cas1,cas2,DinG	1	10	0	0

1. CP024736

CRISPR-Cas detection and classification

Crispr_ID: CP024736_1

• CRISPR_ID: CP024736_1
• CRISPR_location: 1743874-1745491
• CRISPR_type: TypeII
• Repeat_type: NA
• Spacer_info: 24 spacers
• Cas_protein_info: cas2,cas1,cas9

Consensus repeat of CP024736_1

Consensus_repeat	Method
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	CRISPRCasFinder
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	CRT
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	PILER-CR

spacers of CP024736_1

>1.1|1743910|30|CP024736|CRISPRCasFinder,CRT
ATAGCTGGCAAGCATTAAATGAAAATCCGC
>1.2|1743976|30|CP024736|CRISPRCasFinder,CRT
GCGAAGCGGGGGAGTATTGCGCACAATGTC
>1.3|1744042|30|CP024736|CRISPRCasFinder,CRT
CATGCGCCGCCCTGCCTCGTAGGTCTCGCA
>1.4|1744108|30|CP024736|CRISPRCasFinder,CRT
GCTCGTCCCGCAACTTCCCTAGCGGTACAG
>1.5|1744174|30|CP024736|CRISPRCasFinder,CRT
GGAGGACCCTGACCCTAAGGAGATTCTGTT
>1.6|1744240|30|CP024736|CRISPRCasFinder,CRT,PILER-CR
CCGGGGAATCCGGGGGTGCCAAGCCTGTTT
>1.7|1744306|29|CP024736|CRISPRCasFinder,CRT,PILER-CR
TACATTAGAGATTTGCGCTGGACAAAAGA
>1.8|1744371|30|CP024736|CRISPRCasFinder,CRT,PILER-CR
GGGGGCGTCATCGATTGTGGTGGTGATTTG
>1.9|1744437|30|CP024736|CRISPRCasFinder,CRT,PILER-CR
CCGCTACTACTTAACCCCGGCGGAATGCGA
>1.10|1744503|30|CP024736|CRISPRCasFinder,CRT,PILER-CR
AGGGGCGTCATTGTTAGTGATTTGATTGTT
>1.11|1744569|30|CP024736|CRISPRCasFinder,CRT,PILER-CR
TGTGGTCTATCCTCCCTACTGGTGAAATTG
>1.12|1744635|30|CP024736|CRISPRCasFinder,CRT,PILER-CR
TCGTGAGAGCCGCCGCCGCTAAGTTAAGAG
>1.13|1744701|30|CP024736|CRISPRCasFinder,CRT,PILER-CR
GGTCATCGTAAATGCTCATAATCTTATGTC
>1.14|1744767|30|CP024736|CRISPRCasFinder,CRT,PILER-CR
AAACACATGCCAGAGGATGAAAGGGAATTG
>1.15|1744833|30|CP024736|CRISPRCasFinder,CRT,PILER-CR
TCGTAAGAGCCGCCGCCGCTAAGTTAAGAG
>1.16|1744899|30|CP024736|CRISPRCasFinder,CRT,PILER-CR
CGAACCCGGCAGACCTGCTTTCCCGTATTG
>1.17|1744965|30|CP024736|CRISPRCasFinder,CRT,PILER-CR
CAACCATTATTTGTCAACCATATTTCACGC
>1.18|1745031|30|CP024736|CRISPRCasFinder,CRT,PILER-CR
TTGATCCGCGGCCTAATCCAAAAGTAAATT
>1.19|1745097|29|CP024736|CRISPRCasFinder,CRT,PILER-CR
CATAGACGTTTCGGGAAAAGTCCTATATA
>1.20|1745162|30|CP024736|CRISPRCasFinder,CRT,PILER-CR
GTATCCTTAAACTCTTCAATGGTCTTCGGG
>1.21|1745228|30|CP024736|CRISPRCasFinder,CRT,PILER-CR
CCAGCTATTGGTAGAGTGGGGCATTGACAT
>1.22|1745294|30|CP024736|CRISPRCasFinder,CRT,PILER-CR
GCATTTTGCCATTTCCCGCTGATGACTTTT
>1.23|1745360|30|CP024736|CRISPRCasFinder,CRT,PILER-CR
TATTTGCCGAAACGGTATCTGACGCCCGCC
>1.24|1745426|30|CP024736|CRISPRCasFinder,CRT,PILER-CR
AAGGGGTTGTAGGTCAACTGGTCCACAATG

CRISPR arrays and Neighbor proteins around CP024736_1

CRISPR arrays

The CRISPR arrays of CP024736_1

>merge|CP024736|1|1743874-1745491|CRISPRCasFinder,CRT,PILER-CR
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAACATAGCTGGCAAGCATTAAATGAAAATCCGCATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAACGCGAAGCGGGGGAGTATTGCGCACAATGTCATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAACCATGCGCCGCCCTGCCTCGTAGGTCTCGCAATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAACGCTCGTCCCGCAACTTCCCTAGCGGTACAGATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAACGGAGGACCCTGACCCTAAGGAGATTCTGTTATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAACCCGGGGAATCCGGGGGTGCCAAGCCTGTTTATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAACTACATTAGAGATTTGCGCTGGACAAAAGAATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAACGGGGGCGTCATCGATTGTGGTGGTGATTTGATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAACCCGCTACTACTTAACCCCGGCGGAATGCGAATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAACAGGGGCGTCATTGTTAGTGATTTGATTGTTATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAACTGTGGTCTATCCTCCCTACTGGTGAAATTGATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAACTCGTGAGAGCCGCCGCCGCTAAGTTAAGAGATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAACGGTCATCGTAAATGCTCATAATCTTATGTCATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAACAAACACATGCCAGAGGATGAAAGGGAATTGATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAACTCGTAAGAGCCGCCGCCGCTAAGTTAAGAGATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAACCGAACCCGGCAGACCTGCTTTCCCGTATTGATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAACCAACCATTATTTGTCAACCATATTTCACGCATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAACTTGATCCGCGGCCTAATCCAAAAGTAAATTATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAACCATAGACGTTTCGGGAAAAGTCCTATATAATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAACGTATCCTTAAACTCTTCAATGGTCTTCGGGATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAACCCAGCTATTGGTAGAGTGGGGCATTGACATATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAACGCATTTTGCCATTTCCCGCTGATGACTTTTATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAACTATTTGCCGAAACGGTATCTGACGCCCGCCATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAACAAGGGGTTGTAGGTCAACTGGTCCACAATGATTGTACTGTGCCTTATTTTGGATTCAAGACAAAAT

>CP024736|1|1|1743874-1745491|CRISPRCasFinder
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	ATAGCTGGCAAGCATTAAATGAAAATCCGC
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	GCGAAGCGGGGGAGTATTGCGCACAATGTC
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	CATGCGCCGCCCTGCCTCGTAGGTCTCGCA
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	GCTCGTCCCGCAACTTCCCTAGCGGTACAG
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	GGAGGACCCTGACCCTAAGGAGATTCTGTT
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	CCGGGGAATCCGGGGGTGCCAAGCCTGTTT
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	TACATTAGAGATTTGCGCTGGACAAAAGA
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	GGGGGCGTCATCGATTGTGGTGGTGATTTG
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	CCGCTACTACTTAACCCCGGCGGAATGCGA
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	AGGGGCGTCATTGTTAGTGATTTGATTGTT
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	TGTGGTCTATCCTCCCTACTGGTGAAATTG
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	TCGTGAGAGCCGCCGCCGCTAAGTTAAGAG
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	GGTCATCGTAAATGCTCATAATCTTATGTC
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	AAACACATGCCAGAGGATGAAAGGGAATTG
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	TCGTAAGAGCCGCCGCCGCTAAGTTAAGAG
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	CGAACCCGGCAGACCTGCTTTCCCGTATTG
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	CAACCATTATTTGTCAACCATATTTCACGC
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	TTGATCCGCGGCCTAATCCAAAAGTAAATT
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	CATAGACGTTTCGGGAAAAGTCCTATATA
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	GTATCCTTAAACTCTTCAATGGTCTTCGGG
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	CCAGCTATTGGTAGAGTGGGGCATTGACAT
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	GCATTTTGCCATTTCCCGCTGATGACTTTT
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	TATTTGCCGAAACGGTATCTGACGCCCGCC
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	AAGGGGTTGTAGGTCAACTGGTCCACAATG
ATTGTACTGTGCCTTATTTTGGATTCAAGACAAAAT

>CP024736|1|1|1743874-1745491|CRT
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	ATAGCTGGCAAGCATTAAATGAAAATCCGC
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	GCGAAGCGGGGGAGTATTGCGCACAATGTC
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	CATGCGCCGCCCTGCCTCGTAGGTCTCGCA
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	GCTCGTCCCGCAACTTCCCTAGCGGTACAG
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	GGAGGACCCTGACCCTAAGGAGATTCTGTT
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	CCGGGGAATCCGGGGGTGCCAAGCCTGTTT
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	TACATTAGAGATTTGCGCTGGACAAAAGA
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	GGGGGCGTCATCGATTGTGGTGGTGATTTG
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	CCGCTACTACTTAACCCCGGCGGAATGCGA
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	AGGGGCGTCATTGTTAGTGATTTGATTGTT
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	TGTGGTCTATCCTCCCTACTGGTGAAATTG
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	TCGTGAGAGCCGCCGCCGCTAAGTTAAGAG
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	GGTCATCGTAAATGCTCATAATCTTATGTC
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	AAACACATGCCAGAGGATGAAAGGGAATTG
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	TCGTAAGAGCCGCCGCCGCTAAGTTAAGAG
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	CGAACCCGGCAGACCTGCTTTCCCGTATTG
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	CAACCATTATTTGTCAACCATATTTCACGC
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	TTGATCCGCGGCCTAATCCAAAAGTAAATT
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	CATAGACGTTTCGGGAAAAGTCCTATATA
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	GTATCCTTAAACTCTTCAATGGTCTTCGGG
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	CCAGCTATTGGTAGAGTGGGGCATTGACAT
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	GCATTTTGCCATTTCCCGCTGATGACTTTT
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	TATTTGCCGAAACGGTATCTGACGCCCGCC
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	AAGGGGTTGTAGGTCAACTGGTCCACAATG
ATTGTACTGTGCCTTATTTTGGATTCAAGACAAAAT

>CP024736|1|1|1744204-1745491|PILER-CR
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	CCGGGGAATCCGGGGGTGCCAAGCCTGTTT
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	TACATTAGAGATTTGCGCTGGACAAAAGA
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	GGGGGCGTCATCGATTGTGGTGGTGATTTG
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	CCGCTACTACTTAACCCCGGCGGAATGCGA
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	AGGGGCGTCATTGTTAGTGATTTGATTGTT
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	TGTGGTCTATCCTCCCTACTGGTGAAATTG
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	TCGTGAGAGCCGCCGCCGCTAAGTTAAGAG
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	GGTCATCGTAAATGCTCATAATCTTATGTC
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	AAACACATGCCAGAGGATGAAAGGGAATTG
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	TCGTAAGAGCCGCCGCCGCTAAGTTAAGAG
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	CGAACCCGGCAGACCTGCTTTCCCGTATTG
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	CAACCATTATTTGTCAACCATATTTCACGC
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	TTGATCCGCGGCCTAATCCAAAAGTAAATT
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	CATAGACGTTTCGGGAAAAGTCCTATATA
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	GTATCCTTAAACTCTTCAATGGTCTTCGGG
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	CCAGCTATTGGTAGAGTGGGGCATTGACAT
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	GCATTTTGCCATTTCCCGCTGATGACTTTT
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	TATTTGCCGAAACGGTATCTGACGCCCGCC
ATTGTACTGTGCCTTATTTTGGATTCAAGGCAAAAC	AAGGGGTTGTAGGTCAACTGGTCCACAATG
ATTGTACTGTGCCTTATTTTGGATTCAAGACAAAAT

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
CP024736.1|AYR28476.1|1747446_1749348_+|hypothetical-protein	unknown	unknown	gnl|CDD|223861
CP024736.1|AYR28479.1|1750984_1752826_-|molecular-chaperone-HtpG	unknown	unknown	gnl|CDD|235366
CP024736.1|AYR29343.1|1733349_1734165_-|hydroxyethylthiazole-kinase	unknown	unknown	gnl|CDD|236477
CP024736.1|AYR28473.1|1743501_1743813_+|CRISPR-associated-endonuclease-Cas2	cas2	COG3512_cas2_CAS-II-A:CAS-II-B:CAS-II-C	gnl|CDD|226043
CP024736.1|AYR28469.1|1735811_1737098_-|bifunctional-O-acetylhomoserine-aminocarboxypropyltransferase/cysteine-synthase	unknown	unknown	gnl|CDD|225428
CP024736.1|AYR28480.1|1752997_1754869_+|PRTRC-system-protein-C	unknown	unknown	gnl|CDD|379166
CP024736.1|AYR28478.1|1750073_1750901_-|Ion-channel	unknown	unknown	gnl|CDD|369572
CP024736.1|AYR28472.1|1742578_1743514_+|type-II-CRISPR-associated-endonuclease-Cas1	cas1	cd09720_cas1_CAS-II-A:CAS-II-B:CAS-II-C	gnl|CDD|274694
CP024736.1|AYR29344.1|1737232_1737673_-|Rrf2-family-transcriptional-regulator	unknown	unknown	gnl|CDD|273242
CP024736.1|AYR28481.1|1755068_1756253_+|MFS-transporter	unknown	unknown	gnl|CDD|225365
CP024736.1|AYR28467.1|1734212_1734827_-|thiamine-phosphate-synthase	unknown	unknown	gnl|CDD|234590
CP024736.1|AYR28475.1|1746826_1747396_+|hypothetical-protein	unknown	unknown	gnl|CDD|200464
CP024736.1|AYR28470.1|1737765_1738872_-|alanine-racemase	unknown	unknown	gnl|CDD|143481
CP024736.1|AYR28477.1|1749388_1750000_+|hypothetical-protein	unknown	unknown	gnl|CDD|377540
CP024736.1|AYR28483.1|1760044_1761067_+|DUF4105-domain-containing-protein	unknown	unknown	gnl|CDD|379156
CP024736.1|AYR28468.1|1734845_1735775_-|cysteine-synthase-A	unknown	unknown	gnl|CDD|273463
CP024736.1|AYR28466.1|1732575_1733040_-|DUF4065-domain-containing-protein	unknown	unknown	gnl|CDD|226128
CP024736.1|AYR28482.1|1756419_1757445_+|phenylalanine--tRNA-ligase-subunit-alpha	unknown	unknown	gnl|CDD|234780
CP024736.1|AYR28471.1|1739238_1742568_+|hypothetical-protein	cas9	cd09643_cas9_CAS-II-A:CAS-II-B:CAS-II-C	gnl|CDD|379160
CP024736.1|AYR28474.1|1745562_1746633_-|hypothetical-protein	unknown	unknown	gnl|CDD|271177

HMMScan for Signature genes

Protein	Cas_name	Cas_description	E-value	Identity	Coverage
CP024736.1|AYR28471.1|1739238_1742568_+|hypothetical-protein	cas9	cd09643_cas9_CAS-II-A:CAS-II-B:CAS-II-C	1.5e-28	94.4	0.0
CP024736.1|AYR28472.1|1742578_1743514_+|type-II-CRISPR-associated-endonuclease-Cas1	cas1	cd09720_cas1_CAS-II-A:CAS-II-B:CAS-II-C	2.3e-44	146.3	0.0
CP024736.1|AYR28473.1|1743501_1743813_+|CRISPR-associated-endonuclease-Cas2	cas2	COG3512_cas2_CAS-II-A:CAS-II-B:CAS-II-C	1.8e-29	97.1	0.0

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
CP024736.1|AYR28476.1|1747446_1749348_+|hypothetical-protein	gnl|CDD|223861	COG0790, COG0790, FOG: TPR repeat, SEL1 subfamily [General function prediction only].	2.91623e-52
CP024736.1|AYR28479.1|1750984_1752826_-|molecular-chaperone-HtpG	gnl|CDD|235366	PRK05218, PRK05218, heat shock protein 90; Provisional.	0
CP024736.1|AYR29343.1|1733349_1734165_-|hydroxyethylthiazole-kinase	gnl|CDD|236477	PRK09355, PRK09355, hydroxyethylthiazole kinase; Validated.	2.92118e-101
CP024736.1|AYR28473.1|1743501_1743813_+|CRISPR-associated-endonuclease-Cas2	gnl|CDD|226043	COG3512, COG3512, CRISPR-associated protein, Cas2 homolog [Defense mechanisms].	9.33042e-28
CP024736.1|AYR28469.1|1735811_1737098_-|bifunctional-O-acetylhomoserine-aminocarboxypropyltransferase/cysteine-synthase	gnl|CDD|225428	COG2873, MET17, O-acetylhomoserine sulfhydrylase [Amino acid transport and metabolism].	0
CP024736.1|AYR28480.1|1752997_1754869_+|PRTRC-system-protein-C	gnl|CDD|379166	pfam13402, Peptidase_M60, Peptidase M60, enhancin and enhancin-like. This family of peptidases contains a zinc metallopeptidase motif (HEXXHX(8,28)E) and possesses mucinase activity. It includes the viral enhancins as well as enhancin-like peptidases from bacterial species. Enhancins are a class of metalloproteases found in some baculoviruses that enhance viral infection by degrading the peritrophic membrane (PM) of the insect midgut. Bacterial enhancins are found to be cytotoxic when compared to viral enhancin, however, suggesting that the bacterial enhancins do not enhance infection in the same way as viral enhancin. Bacterial enhancins may have evolved a distinct biochemical function. These bacterial domains are peptidases targetting host glycoproteins and thus probably play an important role in successful colonisation of both vertebrate mucosal surfaces and the invertebrate digestive tract by both mutualistic and pathogenic microbes. This family has been augmented by a merge with the sequences in the Enhancin Pfam family.	2.09248e-91
CP024736.1|AYR28478.1|1750073_1750901_-|Ion-channel	gnl|CDD|369572	pfam07885, Ion_trans_2, Ion channel. This family includes the two membrane helix type ion channels found in bacteria.	5.51978e-13
CP024736.1|AYR28472.1|1742578_1743514_+|type-II-CRISPR-associated-endonuclease-Cas1	gnl|CDD|274694	TIGR03639, cas1_NMENI, CRISPR-associated endonuclease Cas1, subtype II/NMENI. The CRISPR-associated protein Cas1 is virtually universal to CRISPR systems. CRISPR, an acronym for Clustered Regularly Interspaced Short Palindromic Repeats, is a prokaryotic immunity system for foreign DNA, mostly from phage. CRISPR systems belong to different subtypes, distinguished by both nature of the repeats, the makeup of the cohort of associated Cas proteins, and by molecular phylogeny within the more universal Cas proteins such as this one. This model is of type EXCEPTION and provides more specific information than the EQUIVALOG model TIGR00287. It describes the Cas1 variant of the NMENI subtype of CRISPR/Cas system.	4.43949e-46
CP024736.1|AYR29344.1|1737232_1737673_-|Rrf2-family-transcriptional-regulator	gnl|CDD|273242	TIGR00738, Putative_HTH-type_transcriptional_regulator, Rrf2 family protein. This model represents a superfamily of probable transcriptional regulators. One member, RRF2 of Desulfovibrio vulgaris is an apparent regulatory protein experimentally (MEDLINE:97293189). The N-terminal region appears related to the DNA-binding biotin repressor region of the BirA bifunctional according to results after three rounds of PSI-BLAST with a fairly high stringency. [Unknown function, General].	2.99251e-42
CP024736.1|AYR28481.1|1755068_1756253_+|MFS-transporter	gnl|CDD|225365	COG2807, CynX, Cyanate permease [Inorganic ion transport and metabolism].	1.72535e-69
CP024736.1|AYR28467.1|1734212_1734827_-|thiamine-phosphate-synthase	gnl|CDD|234590	PRK00043, thiE, thiamine phosphate synthase.	3.31757e-79
CP024736.1|AYR28475.1|1746826_1747396_+|hypothetical-protein	gnl|CDD|200464	cd11325, AmyAc_GTHase, Alpha amylase catalytic domain found in Glycosyltrehalose trehalohydrolase (also called Maltooligosyl trehalose Trehalohydrolase). Glycosyltrehalose trehalohydrolase (GTHase) was discovered as part of a coupled system for the production of trehalose from soluble starch. In the first half of the reaction, glycosyltrehalose synthase (GTSase), an intramolecular glycosyl transferase, converts the glycosidic bond between the last two glucose residues of amylose from an alpha-1,4 bond to an alpha-1,1 bond, making a non-reducing glycosyl trehaloside. In the second half of the reaction, GTHase cleaves the alpha-1,4 glycosidic bond adjacent to the trehalose moiety to release trehalose and malto-oligosaccharide. Like isoamylase and other glycosidases that recognize branched oligosaccharides, GTHase contains an N-terminal extension and does not have the conserved calcium ion present in other alpha amylase family enzymes. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. Glycosyltrehalose Trehalohydrolase Maltooligosyltrehalose Trehalohydrolase.	0.00983453
CP024736.1|AYR28470.1|1737765_1738872_-|alanine-racemase	gnl|CDD|143481	cd00430, PLPDE_III_AR, Type III Pyridoxal 5-phosphate (PLP)-Dependent Enzyme Alanine Racemase. This family includes predominantly bacterial alanine racemases (AR), some serine racemases (SerRac), and putative bifunctional enzymes containing N-terminal UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine ligase (murF) and C-terminal AR domains. These proteins are fold type III PLP-dependent enzymes that play essential roles in peptidoglycan biosynthesis. AR catalyzes the interconversion between L- and D-alanine, which is an essential component of the peptidoglycan layer of bacterial cell walls. SerRac converts L-serine into its D-enantiomer (D-serine) for peptidoglycan synthesis. murF catalyzes the addition of D-Ala-D-Ala to UDPMurNAc-tripeptide, the final step in the synthesis of the cytoplasmic precursor of bacterial cell wall peptidoglycan. Members of this family contain an N-terminal PLP-binding TIM-barrel domain and a C-terminal beta-sandwich domain. They exist as homodimers with active sites that lie at the interface between the TIM barrel domain of one subunit and the beta-sandwich domain of the other subunit. AR and other members of this family require dimer formation and the presence of the PLP cofactor for catalytic activity. Fungal ARs and eukaryotic serine racemases, which are fold types I and II PLP-dependent enzymes respectively, are excluded from this family.	4.38151e-157
CP024736.1|AYR28477.1|1749388_1750000_+|hypothetical-protein	gnl|CDD|377540	pfam05656, DUF805, Protein of unknown function (DUF805). This family consists of several bacterial proteins of unknown function.	2.07476e-24
CP024736.1|AYR28483.1|1760044_1761067_+|DUF4105-domain-containing-protein	gnl|CDD|379156	pfam13387, DUF4105, Domain of unknown function (DUF4105). This is a family of uncharacterized bacterial proteins. There is a highly conserved histidine residue and a well-conserved NCT motif.	1.71804e-25
CP024736.1|AYR28468.1|1734845_1735775_-|cysteine-synthase-A	gnl|CDD|273463	TIGR01136, Cysteine_synthase, cysteine synthase. This model discriminates cysteine synthases (EC 2.5.1.47) (both CysK and CysM) from cystathionine beta-synthase, a protein found primarily in eukaryotes and carrying a C-terminal CBS domain lacking from this protein. Bacterial proteins lacking the CBS domain but otherwise showing resemblamnce to cystathionine beta-synthases and considerable phylogenetic distance from known cysteine synthases were excluded from the seed and score below the trusted cutoff. [Amino acid biosynthesis, Serine family].	3.01928e-168
CP024736.1|AYR28466.1|1732575_1733040_-|DUF4065-domain-containing-protein	gnl|CDD|226128	COG3600, GepA, Uncharacterized phage-associated protein [Function unknown].	1.70878e-18
CP024736.1|AYR28482.1|1756419_1757445_+|phenylalanine--tRNA-ligase-subunit-alpha	gnl|CDD|234780	PRK00488, pheS, phenylalanyl-tRNA synthetase subunit alpha; Validated.	0
CP024736.1|AYR28471.1|1739238_1742568_+|hypothetical-protein	gnl|CDD|379160	pfam13395, HNH_4, HNH endonuclease. This HNH nuclease domain is found in CRISPR-related proteins.	1.5339e-13
CP024736.1|AYR28474.1|1745562_1746633_-|hypothetical-protein	gnl|CDD|271177	cd00796, INT_Rci_Hp1_C, Shufflon-specific DNA recombinase Rci and Bacteriophage Hp1_like integrase, C-terminal catalytic domain. Rci protein is a tyrosine recombinase specifically involved in Shufflon type of DNA rearrangement in bacteria. The shufflon of plasmid R64 consists of four invertible DNA segments which are separated and flanked by seven 19-bp repeat sequences. RCI recombinase facilitates the site-specific recombination between any inverted repeats results in an inversion of the DNA segment(s) either independently or in groups. HP1 integrase promotes site-specific recombination of the HP1 genome into that of Haemophilus influenza. Bacteriophage Hp1_like integrases are tyrosine based site specific recombinases. They belong to the superfamily of DNA breaking-rejoining enzymes, which share the same fold in their catalytic domain and the overall reaction mechanism. The catalytic domain contains six conserved active site residues. Their overall reaction mechanism is essentially identical and involves cleavage of a single strand of a DNA duplex by nucleophilic attack of a conserved tyrosine to give a 3' phosphotyrosyl protein-DNA adduct. In the second rejoining step, a terminal 5' hydroxyl attacks the covalent adduct to release the enzyme and generate duplex DNA.	9.52535e-18

Protein sequences

>CP024736.1|AYR28473.1|1743501_1743813_+|CRISPR-associated-endonuclease-Cas2
MGWLVVFFDLPTTTPEDKKNYQYFRKALLEDGYTMIQYSVYARACVTQERMATHSRRIQKMIPPDGSVRCLFVTNIQWDKMFVFHGRSCPRQEVSLPQQMLLW
>CP024736.1|AYR28472.1|1742578_1743514_+|type-II-CRISPR-associated-endonuclease-Cas1
MSYHILSIDAYTCHLSCDKGQLRCADGENSPRTIPLEDVGAVVLSSFKATLTSNLLIELARKRIGFVLCESYRPAVLLLPADRSTDTGLLRHLADMPARLRNRLWQKTLDAKCGNQTALAQAWNPHHPAIAELKRMAVTEKTAREAECARLFWSVFADTWANSDFRRGRHEEGFNNLFNYAYAILLSCILQYLFALGLDPCFGIFHQSREHAAPLAYDLMEPFRPAFDANVARWIHLCLREGKTEERAGEITREFRQHITATLQASVMYRDKQLPLKAAVEAVCRSFRKAVLAGQSGPYEPWLMTTIKWAG
>CP024736.1|AYR28471.1|1739238_1742568_+|hypothetical-protein
MSRSLTFSFDIGYASIGWAVIASASHDDADPSVCGCGTVLFPKDDCQAFKRREYRRLRRNIRSRRVRIDRIGRLLVQAQIITPEMKETSGHPAPFYLASEALKGHRTLAPIELWHVLRWYAHNRGYDNNASWSNSLSEDGGNGEDTERVKHAQDLMDKHGTATMAETICRELKLEEGKADAPMNVSTPAYKNLNTAFPRLIVEKEVRRILELSAPLIPGLTSEIMELIAQHHPLTTKQRGVLIHYGIKLARRYRGSLLFGQLIPRFDNRIISRCPVTWAQVYEAELKKGNSEQSARERAEKLSKVPTANCPEFYEYRMARILCNIRADGEPLSAEIRRELMNQARQEGKLTKASLEKAISSRLGETETNVANYFTLHPDSEEALYLDPAVEVLKRSGIGETLSPSVYRLAANRLRRGKSVTPNYLLNLLKSRGESVEALEKKIEKERHQQANRKKAKKTDPDYAATPLKPRYATGRAPYARPVLKKVVEETLDGEDPTRPARGEAHPDGELKAHDGCLYCLLDADSSVNQYQKERRLDTMTNNHLVRHRMLILDRLLKDLIQDFADGQKDRISRICVEVGKELTAFSGMGSKKIQTELNLRQKSHTEAVNKLKRDLPGKALSANLIRKCRIAMDMNWTCPFTGERYGTHELENMEMEHIVPYSFRQSNALSSLVLTRKEVNKMKGQRTGYDFVEQEQGKPVTGRTNLHICSFNNYREFVEKLDDKKWHEDDRKRKKKRKALLMVRGLSHRHQLQNHDAMKEIGMTEGMMTQSSHLMKLACKSIKTSLPDAHIDMIPGAVTAEVRKAWDVFGVFKELCPEAADPDSGKILKENLRSLTHLHHALDACVLGLIPYIIPAHHNGLLRRVLAMRRIPEKLIPQVRPVANQRHYVLNDDGRMMLRDLSASLKENIREQLMEQRVIQHVPADMGGALLKETMQRVLSVDGSGEDAMVSLSKKKDGKKKKNQVKASKLVGVFPEGPSKLKALKAAIEIDGNYGVALDPKPVVIRHIKVFKRIMALKEQNGGKPVRILKKGMLIHLTSSKDPKHAGVWRIESIQDSKGGVKLDLQRAHCAVPKNKTHECNWREVDLISLLKKYQMKRYPTSYTGTPR
>CP024736.1|AYR28470.1|1737765_1738872_-|alanine-racemase
MPATSPPRAWAEIDLGAIRHNLNVVKQAAKGEYYMPVVKAGAYGHGLEQVCRTLDSEGIAFFGVANVGEARRISQAGCRTRPYILGPAFPEEREEIVLNGWRSFISTMEEAEHYNSLARLYGKTLPIHLSVDTGMGRGGFLPDQLEELLSRLGELDSLHPEGLGAHLPCADEDREITLRQIALFERMAARIREKLPLKYCHLANSAASLDYEIPSNNMCRPGLVLYGFSPIDSPWASQLRPAMALFSRLTVVRTLPEGHGISYGGTYVTDHPTRVATVGIGYADGYLRSLAHKGARVMVDGVSCPLLGRVTMDQIIVDVSRAPHAEPGMTAEIMGPHIPVTELAEKAGTISWEIFTGIGPRVPRHYNY
>CP024736.1|AYR29344.1|1737232_1737673_-|Rrf2-family-transcriptional-regulator
MKVSTRGRYALRLMIDLAHHRNEGYVSLKEISARQDITVRYLEQIIAILLKAGFVQSFRGKAGGYRLSRHPREYTTEEILKLTEGSLLPISCTAPHENPCPRAAGCATLPFWRGLQQVIENYLRRVTLEDLAEQQKEIGCDYGAGI
>CP024736.1|AYR28469.1|1735811_1737098_-|bifunctional-O-acetylhomoserine-aminocarboxypropyltransferase/cysteine-synthase
MSKNHRFETRQIHVGQESPDPATDARAVPIYATTSYVFKDSEQAAARFALAEPGNIYNRLMNPTADVFEQRIASLEGGAAALAVSTGAAAVTYAIQNIARAGDHIVSSSTVYGGTYNLFANTLADAGIETSFVDARDVQNFSRAIRNNTKALYVESLGNPNCDIVDMEALAEVAHAHGIPLIVDSTFATPFLFRPLEHGADIVVHSATKFIGGHGTVMGGVIVDGGKFDWTQNDKFPGISKPNPNYHGAVFAEVCGNLAYIVKIRATLLRDTGATISPFNSFLLLQGLETLSLRVERHVQNALRVADYLSSHPQVEKVNHPSLPDHPNHDLYKRYYPNGGGSIFTFEIKGGAEKARKFCESLELFSLLANVADVKSLVIHPASTTHSQMTEEELKAGGITPSTVRLSIGTEHIDDILEDLEQGFRAIL
>CP024736.1|AYR28468.1|1734845_1735775_-|cysteine-synthase-A
MRIYKNITELIGGTPLLELTNYERRNGLNATILAKLEYLNPAGSVKDRIAQAMIDAAEAAGKLKPDSVIIEPTSGNTGIGLAAVAASRGYRIILTMPETMSVERRNLLKAYGAELVLTDGALGMKGAIAKAEELAAGLPNSFIPGQFANPANPEAHFRTTGPEIWNDTEGKADIFVAGVGTGGTITGVGKYLKSRNPGIRVVAVEPFSSPVLTQGTAGPHKIQGIGAGFVPETLDTSVYDEVITVTNEDAFSTGKELARTDGVLTGISSGAALWAATQVARRPENEGKTIVVLLPDTGDRYLSTPLFTD
>CP024736.1|AYR28467.1|1734212_1734827_-|thiamine-phosphate-synthase
MKKFNLHLYLVTDEASKCRYSLLETVRKAADGGVTMVQYRSTNPDAGTCYREALPIRDFLASRGIPFIVNNRIDLALALEADGVHIGQRDLPVPSVRAMIGPDKILGLSVSNKEQLRAVDASLVDYLGMGPVFPTISKLNAPPVLSVEGFAALASQSPLPVVAIGGLDAERARQVRATGTASGIAVVSAICGAENPEAAARALA
>CP024736.1|AYR29343.1|1733349_1734165_-|hydroxyethylthiazole-kinase
MLSPTDLVQAVAADLEKIRETAPLVLSLTNSVVQPLTANLLLAIGAVPAMLNDAEEAADMLRSGTGALLVNLGTVTREQGAAMQTAVREANRLNIPWVLDPVAVGALSLRTRLAGQLKEQSPRIIRGNASEIMALAGYSSVTKGPESTSSSADALHAARELALHTGAAVLVTGRTDYSTDGRQVTATENGHAMMSRVTGVGCSMGALSAACAAVSPTPLQAAVSTAVLMGIAGEMAFEQSPFPGSFAVSLLDSLYALSPEDVVRRARFLSL
>CP024736.1|AYR28466.1|1732575_1733040_-|DUF4065-domain-containing-protein
MANSTTTQKLCNWFIQQSNARGHVLTPVKLNHLVILADWWHLHRTGGFLINERVEAWPSGPVLPSIYHEYKDQPPYSSIEHPSRRQPPLEEETDIIPFLEHIWNFYGKYTAQQLGRINTAPSSPWRQAQGRHEQAHRQQITEEHVQAYFQSLAG
>CP024736.1|AYR28474.1|1745562_1746633_-|hypothetical-protein
MNIPTSSFPSDTDQTGILLIRSLGIPPMDAFLLLKDLLDTSRGRGDRITRAKRCIRLGGEALADRETSVPFSQAVRASLEARKHRRPRTLQEIRYMAARMMKKCPELARKQVRSITPEDCGRYLRKSFSTPRQRHKGRLILSGILNFSLKRGWCRRNAAFLVPPPILREKRIRALSLYEAKRLLHTAEQLFHGACLPACALMLYAGIRPHEVKRLTWKNINLKSGLVSLAPCHTKTGGSRHVSILPVLGAILSRTSSAGSPSRPVCPPNWEKKWREVRRRSGILKKSGWVQDVLRHTYASYHLAHFCNQNLLQKEMGHSSPSLLLARYLNMDGITSATGAMFWTYSFIPPAPLKKD
>CP024736.1|AYR28475.1|1746826_1747396_+|hypothetical-protein
MNVSISLLIIFGTVFLVGEHVLARTLETGRAGGYYTHSGKGDRSARARFERFKSRKTPTTRTSITPISVSDQYGNRYRGDLITSTYDPSAKARAGAAAWDKRQSAKAKTIINQAEHEAQSKIAREEEAQVFRASLSRLQEAVKQGKVDKVLADKMVKVLSLRVKQGIMDRREAAGAIDYLILNNEINIK
>CP024736.1|AYR28476.1|1747446_1749348_+|hypothetical-protein
MSMLHAILSILVGALGGLSARVIFSFAASVVLLPLSLFSSRRETQVSTVQAALALCLLVIAVSLYDFAVIKSWKGIVEHLTWGDYIGIVVFCVSSACIPDSRAADMAGDCKYNSMILNAGVYFSLFIPVGFLLMDCSENMWSYLSFIIVFLLFSFFLIDFHCSKKSMKQIFADGNNYPEIEIEKKRWAGRKWRMIAIIISVCFLTAFSVVQLFNGKKYAHENETVELDHTRLMDLGEQYLNKGDVERAVSCFKKSAEFGNAEAQRMLGYCYFWGEGIREDKQEAVKWYRLAAGQGNAEAQRMLGYCYFWGEGIREDKQEAVKWYRLAADQGDAEAQWMLGDCYYFGTGIREDKQEAVKWYRLAAGQGNAEAQRRLGYCYYVGKGIREDKREAVKWYRLAADQGNAEAQWRLGYCYYVGKGIREDKREAVKWYRLAAGQGNAEAQRRLGYFYFWGKGIREDKQEAVKWYRLAAGQGNAEAQRRLGYCYFWGEGIREDKQEAVKWYRLAADQGDAEAQRRLGYCYFWGEGIREDKQEAVKWYRLAADQGNAEAQRWLGDCYFFGHGVGKNKREAVNWYFLSAEKGNAEAQRRLGACFYFGHGVAKNRQEAIRWYRLAAEQGNSVAISMLKKLGVM
>CP024736.1|AYR28477.1|1749388_1750000_+|hypothetical-protein
MKVMEQSEERLSRINAYNKLVDAAILTKTSSKSVADIVNHELGIECNAANVQEILKSSVSASDMHKIRREIEENASIKMENANGTSFLRKYVSFSGRISRSEYWALKLLILPIMVIILLNGVAMVWDKTGNDSGILVLFVIIFISYIYFNIVADVRRLHDIGESGWLVLLELVPLVNIVIAAFLFFKGSQSAGNQHKDLKSSH
>CP024736.1|AYR28478.1|1750073_1750901_-|Ion-channel
MNALRRMPRHILFLRLAYQAGMCLLALVAVTLTAIDLTTEAAEWEVTADRVIYWIFVLDYVVRFSISRSKWLFVKEHPWDLLAIIPFDALFRLFRFASLGEILRLARYLDLFSYAMRFTTRIRRFFNTNGFKYICIAALVIILLGAVGIHLAEGMSLPNGIWWSFVTATTVGYGDTYPVTTSGKFLAVFLMLTGIGFVGTLTSTITSFFLSPHGGEALPYREEEIEAIKKKLDDLSSMTDEDIDTMAALLKTLRDASPAPSAGKEETPAIHSEKN
>CP024736.1|AYR28479.1|1750984_1752826_-|molecular-chaperone-HtpG
MNTETHQFQAEVRQLLDIVINALYSDREIFVRELVSNASDALEKLRLKQLTDSNIYQPDKPLEITVTTDKENKTITIADTGIGMTEADLVENLGTIAHSGTKKFMEALKQKQEGGADLIGQFGVGFYSSFMVADRVEVFTHSYEPEAASLRWSSDGREGYSIETLAEPLDRGTRIVIRLKDEYEEFSQEYRVKELLRRYSNFVGFPLNFNGERVNTVQAIWSKSKSDVKPEEYDEFYQFIAHTDEKPLSYMHFSADAPIALNALLFIPRRNPEMFGFGRVDANVALYCKRVLIDAKPEGLLPEWLRFLNGVVDSEDLPLNISREMLQDNSLVRKISDIITKRFIKHLEKLAKDDQETYREFYAQFSRYLKEGVVTSWPNKESLGKLLRFESTATEPGETTSFEDYVTRMKEGQTAIYALTGPSRSHLENSPYLEAFKARGYEVAFFTDHGDEFVLDSLSSVDGKPVTMIDRADVELPALEEEQKDALPQEEAAALEEWLKGLYPDKFSKVTLGKRLVSGAAVALQSGNDMGPEMRAYMKAMGQEVPESHPQLELNPSNPLVKKLSALRTENPELAQMVADQIANTALLRAGMLDDPAVLAQSSQALMEQLLLK
>CP024736.1|AYR28480.1|1752997_1754869_+|PRTRC-system-protein-C
MIFSPLLKKGILLLAFCGVLGGGVLSAQETGGSPITEEGLKALDSNVFANEYMLALKPNVTRDRIAKIKDPFVRGLAEQMAEKKFDRKARALTAEPYEPVKDLAERLRTSQYSKFENPTGIFFEEGEDVLLVMGDPKGEKLNLVIHNFGRDGGHSSYPLKEGVNIIRAKNKGLGYIEYFTSNYKKAPKVHLSILSGKVNGVFVGGVSKNSDWKRMLENSPTEVVDIVGNRVHLVYPVEELKQFCPDKGEELIALYDRIIGMEQQIMGLYKYRMLPKNRMFGRVIWNGFMHADGTGAAFHNGTMKEVGNPDRIPGSAWGIAHEFGHVNQVRPAMKWVSTGEVTNNIYSAYVNYMLNPSSMRLEHERINGGDGNMIGGRFNAYLNNGILKGENWLVQSGPDKRSGGDNRPMVHDHFVKLAPLWQLELYFKIAGKGNPDFYPDIFYKAIKMDTRGKKDGELQLAFMKNACDAARQDLTDFFRKTGMLKPIDQELDDYTCARMTITEADCKNLIAYARKYKKPESPVIYYISVNSAEAYKNRLPVRGVYNQGVTEQGNRRIISHDVWKNAVVFETYKDREMVRITMAGTDSRDNSSTTVPYPEGSTRIEAVSWDGRRTLVYGKRPAK
>CP024736.1|AYR28481.1|1755068_1756253_+|MFS-transporter
MDKAGTSGGIRGKMLLFAAFLIVCFNLRTGFDSPDPLLGTIERDMGLSLENSGLFALLPVFVLGVAAPISPRVARRMTPWKIIFWFQLLAVAGIFWRSWDGVAGLYGGMVLMGLGMGIAGAAIPGLIKHQFPDRASAMMGVYSAMIGVGSAAASGLSVPLSNMLGGWRFGLGIWIIPILLGMLVWGAYFLRHPDGVFQNDPAASGRSLLRSGKAWQVTVFYLSRVGAAYFFYTWIPIFLRQRGMSYEDAGFILSVAMFAQLPATLSAHVLEKAMGGRGLLIVMAMALAALSCWGILYLPLDWAVWMAILFGLATGTVFSRGMALMVERARTPSESIRLSGMSQGIGFTMGALLSLLFTSFLHQGGSFLPFCLVYTLFCVLGMVSGRMSARPGYV
>CP024736.1|AYR28482.1|1756419_1757445_+|phenylalanine--tRNA-ligase-subunit-alpha
MKEEIVRIQRDALARIAQASDRRGVEDARVAILGKKGELTLAQTGMKDVPREEKPAVGQLLNEARKAITEALDAKLEEVQAQADKAAVAGVDLTLPARSLPSGGLHPLTIVRDEAVRILRHMGFALADGPEIEDEFHCFDALNTPEDHPARNEKDTFYFDSGKLLRTHTSSVQIRSMEKQLPPVRVIAPGSAYRRDEIDATHLSAFNQLEGLYVDTDVSVGDLKGTLEYFLRALFGSGTEVRFRPHFFPFTEPSFEIDVKLKVDGQAPRWVEIAGCGMVDPNVFEAVDRELGLDPGAQARYTGLTGFAFGIGLDRLAMIRWGIRDIRALIENDVRFLAQFQ
>CP024736.1|AYR28483.1|1760044_1761067_+|DUF4105-domain-containing-protein
MHLTFLRHAGLAAARILMMAAWFGLSVWAAGVVFYNVWGGPVLVWLYVAAMACAFALRRKRPVLWRASWGVPALLLAYYLCIPATNDKEWQPSWSRLPSVEINGNEIVVKDVRSFIYRTERDFDARYVTRRFDLDKLATLDFAVSHWDGMEFVAHTMLSFGFEDGKHLALSVETRLPEGVEQGSVPGLYKQFNVIYILADEEDLFALRTNYRKEDMYLYRINIDRENLKKAFLGFAEKINSLHERPRYYHTVTANCTTELVDTFKNYLGVRRWQWTPVFNGMCDQNAYDRGELLHLPGESFRELKKRSFMGHGGNGEDWPALRRRWEEGWRTFASAPVKE

Crispr_ID: CP024736_2

• CRISPR_ID: CP024736_2
• CRISPR_location: 2171303-2171852
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 8 spacers

Consensus repeat of CP024736_2

Consensus_repeat	Method
ACTTCAATTCACGCGCCCGTGAGGGTGCGAC	PILER-CR
ACTTCAATTCACGCGCCCGTGAGGGTGCGAC	CRISPRCasFinder
ACTTCAATTCACGCGCCCGTGAGGGTGCGAC	CRT

spacers of CP024736_2

>2.1|2171334|34|CP024736|PILER-CR,CRISPRCasFinder,CRT
TCAAGGCGATTGATTGACCAGTTCGGCAAGTAGT
>2.2|2171399|34|CP024736|PILER-CR,CRISPRCasFinder,CRT
AGGATGCCGCTAGGTTGCAGTTCCTCTATCATGG
>2.3|2171464|34|CP024736|PILER-CR,CRISPRCasFinder,CRT
AGCCAACCGCTTATGACGTTCTCGCTTGCCTGAC
>2.4|2171529|34|CP024736|PILER-CR,CRISPRCasFinder,CRT
AAAACGATTGTGCGAAACAGTGAGGCCCCATACA
>2.5|2171594|34|CP024736|PILER-CR,CRISPRCasFinder,CRT
CCTGATGAACCAAGCTTGGCAGTTACTCATCAAC
>2.6|2171659|33|CP024736|PILER-CR,CRISPRCasFinder,CRT
CCGTCCCCCGGGGCCTGATTTGACAACCTGCGA
>2.7|2171723|34|CP024736|PILER-CR,CRISPRCasFinder,CRT
CGTTTCCGGGTTTTCGATTATGCGCACTAGGCCC
>2.8|2171788|34|CP024736|PILER-CR,CRISPRCasFinder,CRT
GTCAGGGCACCAGCGAGTTACAACGCAATAAAAA

CRISPR arrays and Neighbor proteins around CP024736_2

CRISPR arrays

The CRISPR arrays of CP024736_2

>merge|CP024736|2|2171303-2171852|PILER-CR,CRISPRCasFinder,CRT
ACTTCAATTCACGCGCCCGTGAGGGTGCGACTCAAGGCGATTGATTGACCAGTTCGGCAAGTAGTACTTCAATTCACGCGCCCGTGAGGGTGCGACAGGATGCCGCTAGGTTGCAGTTCCTCTATCATGGACTTCAATTCACGCGCCCGTGAGGGTGCGACAGCCAACCGCTTATGACGTTCTCGCTTGCCTGACACTTCAATTCACGCGCCCGTGAGGGTGCGACAAAACGATTGTGCGAAACAGTGAGGCCCCATACAACTTCAATTCACGCGCCCGTGAGGGTGCGACCCTGATGAACCAAGCTTGGCAGTTACTCATCAACACTTCAATTCACGCGCCCGTGAGGGTGCGACCCGTCCCCCGGGGCCTGATTTGACAACCTGCGAACTTCAATTCACGCGCCCGTGAGGGTGCGACCGTTTCCGGGTTTTCGATTATGCGCACTAGGCCCACTTCAATTCACGCGCCCGTGAGGGTGCGACGTCAGGGCACCAGCGAGTTACAACGCAATAAAAAACTTCAATTCACGCGCCCGTGAGGGTGCGAC

>CP024736|2|2|2171303-2171852|PILER-CR
ACTTCAATTCACGCGCCCGTGAGGGTGCGAC	TCAAGGCGATTGATTGACCAGTTCGGCAAGTAGT
ACTTCAATTCACGCGCCCGTGAGGGTGCGAC	AGGATGCCGCTAGGTTGCAGTTCCTCTATCATGG
ACTTCAATTCACGCGCCCGTGAGGGTGCGAC	AGCCAACCGCTTATGACGTTCTCGCTTGCCTGAC
ACTTCAATTCACGCGCCCGTGAGGGTGCGAC	AAAACGATTGTGCGAAACAGTGAGGCCCCATACA
ACTTCAATTCACGCGCCCGTGAGGGTGCGAC	CCTGATGAACCAAGCTTGGCAGTTACTCATCAAC
ACTTCAATTCACGCGCCCGTGAGGGTGCGAC	CCGTCCCCCGGGGCCTGATTTGACAACCTGCGA
ACTTCAATTCACGCGCCCGTGAGGGTGCGAC	CGTTTCCGGGTTTTCGATTATGCGCACTAGGCCC
ACTTCAATTCACGCGCCCGTGAGGGTGCGAC	GTCAGGGCACCAGCGAGTTACAACGCAATAAAAA
ACTTCAATTCACGCGCCCGTGAGGGTGCGAC

>CP024736|2|2|2171303-2171852|CRISPRCasFinder
ACTTCAATTCACGCGCCCGTGAGGGTGCGAC	TCAAGGCGATTGATTGACCAGTTCGGCAAGTAGT
ACTTCAATTCACGCGCCCGTGAGGGTGCGAC	AGGATGCCGCTAGGTTGCAGTTCCTCTATCATGG
ACTTCAATTCACGCGCCCGTGAGGGTGCGAC	AGCCAACCGCTTATGACGTTCTCGCTTGCCTGAC
ACTTCAATTCACGCGCCCGTGAGGGTGCGAC	AAAACGATTGTGCGAAACAGTGAGGCCCCATACA
ACTTCAATTCACGCGCCCGTGAGGGTGCGAC	CCTGATGAACCAAGCTTGGCAGTTACTCATCAAC
ACTTCAATTCACGCGCCCGTGAGGGTGCGAC	CCGTCCCCCGGGGCCTGATTTGACAACCTGCGA
ACTTCAATTCACGCGCCCGTGAGGGTGCGAC	CGTTTCCGGGTTTTCGATTATGCGCACTAGGCCC
ACTTCAATTCACGCGCCCGTGAGGGTGCGAC	GTCAGGGCACCAGCGAGTTACAACGCAATAAAAA
ACTTCAATTCACGCGCCCGTGAGGGTGCGAC

>CP024736|2|2|2171303-2171852|CRT
ACTTCAATTCACGCGCCCGTGAGGGTGCGAC	TCAAGGCGATTGATTGACCAGTTCGGCAAGTAGT
ACTTCAATTCACGCGCCCGTGAGGGTGCGAC	AGGATGCCGCTAGGTTGCAGTTCCTCTATCATGG
ACTTCAATTCACGCGCCCGTGAGGGTGCGAC	AGCCAACCGCTTATGACGTTCTCGCTTGCCTGAC
ACTTCAATTCACGCGCCCGTGAGGGTGCGAC	AAAACGATTGTGCGAAACAGTGAGGCCCCATACA
ACTTCAATTCACGCGCCCGTGAGGGTGCGAC	CCTGATGAACCAAGCTTGGCAGTTACTCATCAAC
ACTTCAATTCACGCGCCCGTGAGGGTGCGAC	CCGTCCCCCGGGGCCTGATTTGACAACCTGCGA
ACTTCAATTCACGCGCCCGTGAGGGTGCGAC	CGTTTCCGGGTTTTCGATTATGCGCACTAGGCCC
ACTTCAATTCACGCGCCCGTGAGGGTGCGAC	GTCAGGGCACCAGCGAGTTACAACGCAATAAAAA
ACTTCAATTCACGCGCCCGTGAGGGTGCGAC

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
CP024736.1|AYR28776.1|2175112_2175376_+|30S-ribosomal-protein-S20	unknown	unknown	gnl|CDD|376586
CP024736.1|AYR28774.1|2170274_2171108_-|secretion-protein	unknown	unknown	gnl|CDD|367948
CP024736.1|AYR28782.1|2180288_2181071_-|SDR-family-NAD(P)-dependent-oxidoreductase	unknown	unknown	gnl|CDD|223696
CP024736.1|AYR28779.1|2177010_2178141_+|tRNA-dihydrouridine-synthase-family-protein	unknown	unknown	gnl|CDD|239200
CP024736.1|AYR28771.1|2162619_2163507_+|hypothetical-protein	unknown	unknown	gnl|CDD|237030
CP024736.1|AYR28773.1|2169057_2169954_-|phosphatidylserine-decarboxylase	unknown	unknown	gnl|CDD|179097
CP024736.1|AYR28781.1|2179289_2180147_+|hypothetical-protein	unknown	unknown	gnl|CDD|224513
CP024736.1|AYR29382.1|2172340_2174194_-|biosynthetic-type-acetolactate-synthase-large-subunit	unknown	unknown	gnl|CDD|215261
CP024736.1|AYR28770.1|2161336_2162539_-|carboxynorspermidine-decarboxylase	unknown	unknown	gnl|CDD|143502
CP024736.1|AYR28778.1|2175812_2177012_-|4-hydroxy-3-methylbut-2-enyl-diphosphate-reductase	unknown	unknown	gnl|CDD|237367
CP024736.1|AYR28780.1|2178153_2179293_+|transglutaminase-domain-containing-protein	unknown	unknown	gnl|CDD|376628
CP024736.1|AYR28772.1|2168275_2168929_+|ribulose-phosphate-3-epimerase	unknown	unknown	gnl|CDD|238244
CP024736.1|AYR28783.1|2181138_2182014_-|bifunctional-5,10-methylene-tetrahydrofolate-dehydrogenase/5,10-methylene-tetrahydrofolate-cyclohydrolase	unknown	unknown	gnl|CDD|223268
CP024736.1|AYR28777.1|2175456_2175678_+|hypothetical-protein	unknown	unknown	unknown
CP024736.1|AYR29380.1|2163822_2165487_-|sulfatase	unknown	unknown	gnl|CDD|293763
CP024736.1|AYR28775.1|2174343_2174952_-|beta-phosphoglucomutase-family-hydrolase	unknown	unknown	gnl|CDD|223710
CP024736.1|AYR28768.1|2155746_2157885_-|glycoside-hydrolase-family-2-protein	unknown	unknown	gnl|CDD|236657
CP024736.1|AYR29379.1|2159375_2161313_-|biosynthetic-arginine-decarboxylase	unknown	unknown	gnl|CDD|235427
CP024736.1|AYR28769.1|2158204_2159305_+|site-specific-integrase	unknown	unknown	gnl|CDD|271175
CP024736.1|AYR29381.1|2165677_2168251_+|leucine--tRNA-ligase	unknown	unknown	gnl|CDD|234743

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
CP024736.1|AYR28776.1|2175112_2175376_+|30S-ribosomal-protein-S20	gnl|CDD|376586	pfam01649, Ribosomal_S20p, Ribosomal protein S20. Bacterial ribosomal protein S20 interacts with 16S rRNA.	1.24995e-16
CP024736.1|AYR28774.1|2170274_2171108_-|secretion-protein	gnl|CDD|367948	pfam04450, BSP, Peptidase of plants and bacteria. These basic secretory proteins (BSPs) are believed to be part of the plants defense mechanism against pathogens.	1.11966e-62
CP024736.1|AYR28779.1|2177010_2178141_+|tRNA-dihydrouridine-synthase-family-protein	gnl|CDD|239200	cd02801, DUS_like_FMN, Dihydrouridine synthase-like (DUS-like) FMN-binding domain. Members of this family catalyze the reduction of the 5,6-double bond of a uridine residue on tRNA. Dihydrouridine modification of tRNA is widely observed in prokaryotes and eukaryotes, and also in some archaea. Most dihydrouridines are found in the D loop of t-RNAs. The role of dihydrouridine in tRNA is currently unknown, but may increase conformational flexibility of the tRNA. It is likely that different family members have different substrate specificities, which may overlap. 1VHN, a putative flavin oxidoreductase, has high sequence similarity to DUS. The enzymatic mechanism of 1VHN is not known at the present.	1.6243e-54
CP024736.1|AYR28771.1|2162619_2163507_+|hypothetical-protein	gnl|CDD|237030	PRK12270, kgd, multifunctional oxoglutarate decarboxylase/oxoglutarate dehydrogenase thiamine pyrophosphate-binding subunit/dihydrolipoyllysine-residue succinyltransferase subunit.	0.00530894
CP024736.1|AYR28773.1|2169057_2169954_-|phosphatidylserine-decarboxylase	gnl|CDD|179097	PRK00723, PRK00723, phosphatidylserine decarboxylase; Provisional.	2.32887e-125
CP024736.1|AYR28781.1|2179289_2180147_+|hypothetical-protein	gnl|CDD|224513	COG1597, LCB5, Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase [Lipid metabolism / General function prediction only].	1.77322e-43
CP024736.1|AYR29382.1|2172340_2174194_-|biosynthetic-type-acetolactate-synthase-large-subunit	gnl|CDD|215261	PLN02470, PLN02470, acetolactate synthase.	0
CP024736.1|AYR28770.1|2161336_2162539_-|carboxynorspermidine-decarboxylase	gnl|CDD|143502	cd06829, PLPDE_III_CANSDC, Type III Pyridoxal 5-phosphate (PLP)-Dependent Enzyme Carboxynorspermidine Decarboxylase. Carboxynorspermidine decarboxylase (CANSDC) catalyzes the decarboxylation of carboxynorspermidine, the last step in the biosynthesis of norspermidine. It is homologous to eukaryotic ornithine decarboxylase (ODC) and diaminopimelate decarboxylase (DapDC), which are fold type III PLP-dependent enzymes that contain an N-terminal PLP-binding TIM-barrel domain and a C-terminal beta-sandwich domain, similar to bacterial alanine racemases. Based on this similarity, CANSDC may require homodimer formation and the presence of the PLP cofactor for its catalytic activity.	0
CP024736.1|AYR28778.1|2175812_2177012_-|4-hydroxy-3-methylbut-2-enyl-diphosphate-reductase	gnl|CDD|237367	PRK13371, PRK13371, 4-hydroxy-3-methylbut-2-enyl diphosphate reductase; Provisional.	0
CP024736.1|AYR28780.1|2178153_2179293_+|transglutaminase-domain-containing-protein	gnl|CDD|376628	pfam01841, Transglut_core, Transglutaminase-like superfamily. This family includes animal transglutaminases and other bacterial proteins of unknown function. Sequence conservation in this superfamily primarily involves three motifs that centre around conserved cysteine, histidine, and aspartate residues that form the catalytic triad in the structurally characterized transglutaminase, the human blood clotting factor XIIIa'. On the basis of the experimentally demonstrated activity of the Methanobacterium phage pseudomurein endoisopeptidase, it is proposed that many, if not all, microbial homologs of the transglutaminases are proteases and that the eukaryotic transglutaminases have evolved from an ancestral protease.	2.73258e-12
CP024736.1|AYR28772.1|2168275_2168929_+|ribulose-phosphate-3-epimerase	gnl|CDD|238244	cd00429, RPE, Ribulose-5-phosphate 3-epimerase (RPE). This enzyme catalyses the interconversion of D-ribulose 5-phosphate (Ru5P) into D-xylulose 5-phosphate, as part of the Calvin cycle (reductive pentose phosphate pathway) in chloroplasts and in the oxidative pentose phosphate pathway. In the Calvin cycle Ru5P is phosphorylated by phosphoribulose kinase to ribulose-1,5-bisphosphate, which in turn is used by RubisCO (ribulose-1,5-bisphosphate carboxylase/oxygenase) to incorporate CO2 as the central step in carbohydrate synthesis.	2.30931e-105
CP024736.1|AYR28783.1|2181138_2182014_-|bifunctional-5,10-methylene-tetrahydrofolate-dehydrogenase/5,10-methylene-tetrahydrofolate-cyclohydrolase	gnl|CDD|223268	COG0190, FolD, 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase [Coenzyme metabolism].	2.62007e-142
CP024736.1|AYR28782.1|2180288_2181071_-|SDR-family-NAD(P)-dependent-oxidoreductase	gnl|CDD|223696	COG0623, FabI, Enoyl-[acyl-carrier-protein].	2.77822e-127
CP024736.1|AYR29380.1|2163822_2165487_-|sulfatase	gnl|CDD|293763	cd16144, ARS_like, uncharacterized arylsulfatase subfamily. Sulfatases catalyze the hydrolysis of sulfate esters from wide range of substrates, including steroids, carbohydrates and proteins. Sulfate esters may be formed from various alcohols and amines. The biological roles of sulfatase includes the cycling of sulfur in the environment, in the degradation of sulfated glycosaminoglycans and glycolipids in the lysosome, and in remodeling sulfated glycosaminoglycans in the extracellular space. The sulfatases are essential for human metabolism. At least eight human monogenic diseases are caused by the deficiency of individual sulfatases.	1.78935e-173
CP024736.1|AYR28775.1|2174343_2174952_-|beta-phosphoglucomutase-family-hydrolase	gnl|CDD|223710	COG0637, COG0637, Predicted phosphatase/phosphohexomutase [General function prediction only].	2.72666e-53
CP024736.1|AYR28768.1|2155746_2157885_-|glycoside-hydrolase-family-2-protein	gnl|CDD|236657	PRK10150, PRK10150, beta-D-glucuronidase; Provisional.	6.66008e-54
CP024736.1|AYR29379.1|2159375_2161313_-|biosynthetic-arginine-decarboxylase	gnl|CDD|235427	PRK05354, PRK05354, biosynthetic arginine decarboxylase.	0
CP024736.1|AYR28769.1|2158204_2159305_+|site-specific-integrase	gnl|CDD|271175	cd00397, DNA_BRE_C, DNA breaking-rejoining enzymes, C-terminal catalytic domain. The DNA breaking-rejoining enzyme superfamily includes type IB topoisomerases and tyrosine based site-specific recombinases (integrases) that share the same fold in their catalytic domain containing conserved active site residues. The best-studied members of this diverse superfamily include Human topoisomerase I, the bacteriophage lambda integrase, the bacteriophage P1 Cre recombinase, the yeast Flp recombinase, and the bacterial XerD/C recombinases. Their overall reaction mechanism is essentially identical and involves cleavage of a single strand of a DNA duplex by nucleophilic attack of a conserved tyrosine to give a 3' phosphotyrosyl protein-DNA adduct. In the second rejoining step, a terminal 5' hydroxyl attacks the covalent adduct to release the enzyme and generate duplex DNA. The enzymes differ in that topoisomerases cleave and then rejoin the same 5' and 3' termini, whereas a site-specific recombinase transfers a 5' hydroxyl generated by recombinase cleavage to a new 3' phosphate partner located in a different duplex region. Many DNA breaking-rejoining enzymes also have N-terminal domains, which show little sequence or structure similarity.	1.00372e-12
CP024736.1|AYR29381.1|2165677_2168251_+|leucine--tRNA-ligase	gnl|CDD|234743	PRK00390, leuS, leucyl-tRNA synthetase; Validated.	0

Protein sequences

>CP024736.1|AYR28774.1|2170274_2171108_-|secretion-protein
MKRYCLVSGLCLCLFMGIFSCIPEAPEHKTEKDASAIVLDEDSDIIWRNFSLGRVSFDDKAPHSKGSRIFHRLIPDTEVYIRQLSRIVLHTLYESPEECIVPVHHLRFMLEDTDGVACKWGGNGEVAISCSTRHIEGLFKDDDDAAKVYFEIRGVLLHELTHVYQQEPQGIGSYGTNREFRAFIEGIADAVRIANGGFHRGAYRPTGGSYMDGYLHAGYFFVWLRDYKDPEFLRKLNRSALEVVPWSFDGAVKYALGSEYHIDELWREYQRVMEEEK
>CP024736.1|AYR28773.1|2169057_2169954_-|phosphatidylserine-decarboxylase
MEGIRYYNRYTGREEREQVPGEKYMQWIYGTASGKAALHVLIKRGVFSRLLGWLKNRPSSARSIPSFVEEYGINMEDSLKGVGEFRHFNDFFYRRLKPGARPLAGGEDTAVFPADARHMGWERADRIKNVFVKGQRFDLPSLLGSDTLAERYAAGAVVLSRLCPTDYHRFHFPVSGVPGPWTKLGGPLASVSPYCLRGRLAWLWTNKRNLTLIRSELWGEVAMLEVGATGVGLIEETYVPGVFSARGAEKGYFAFGGSTVMCFFEPGRVSLASDLLEKTEEGLELFARQGDMMGTAAT
>CP024736.1|AYR28772.1|2168275_2168929_+|ribulose-phosphate-3-epimerase
MTIISPSLLAADFSRIGEEARRAFDSGADWLHLDIMDGHFVDNISFGPEICAAVRKAVGPDRFLDAHLMIERPDHYFDRFVRAGVNLICFHVELGDSYYIRNTLGRIREAGVKNGLALNPATSFEAVEPYLAEIDLLLVMSVVPGFGGQSFMPSVLDKVEKAVEWRTENRASFLIQMDGGIGQNNAAQCALAGADVLVAGSSTFKAPDMARAIAEMK
>CP024736.1|AYR29381.1|2165677_2168251_+|leucine--tRNA-ligase
MSERKKPYPFDVFEPKWQQIWDERKTFKVNNPGEEGFDASKPKYYVLDMFPYPSGAGLHVGHPEGYTATDIVARFKRMNGFNVLHPMGWDSFGLPAEQYAIKTGQHPSVTTFRNIDNFRRQLKMLGFSYDWDREIATTDHEYVRWTQWIFLQLYNSYYNKELKKARPVSELEEQGLSREEIDQRRLAYVAEAAVNWSPDLGTVLANEEVEEWKSKGHRVERRPLRQWMLRITDYAERLIDELEPLDWPESIKLLQRNWIGKSEGAEVDFTLDGETITVYTTRPDTLFGATYMVLSPEHPLVDTVTTPEQKHAVEQYRAQCASKSDLERTDLSKEKTGVFTGAYAVNPVNGKQIPVWIADYVLMGYGTGAIMAVPAHDERDFAFAQVFGLPILQVVQPPSEDMDWRGFCGYEGSSVNSGFLTGLPTPEAKEKMILWLEENGKGRRKVNYKLRDWLFSRQRYWGEPFPIVWENGHHRALPESELPVLQPDLDDFAPTGDPRGPLVKAAEWIAYTPTAHRETNTMPQWAGSCWYYLRYLDPANTERFVSREAEQYWMGSAGSPGGVDLYVGGTEHAVLHLLYARFWHKVLFDLGYLSTNEPFQKLVNQGLILGEDGQKMSKSRGNVVNPDDIVREYGADSLRLYEMFMGPLKDVKPWATKGVEGISRFLARVWRVAFRENQEGEWEINSKLVENAPEAGVLAVRKELHKTIKKVTEDINGMSFNTAIAKMMECTNAMTSADVVDVQDYDAFLTLLNPFAPHLTEEIHSRLQTAFPALAQTQLCQKSWPEWEEALLEENTVPMVIQVNGKLRDKLEVPKDISREELEKQALASAKVKTFLDGVTVRKVIVVPGRLVNIVAN
>CP024736.1|AYR29380.1|2163822_2165487_-|sulfatase
MSAVCGALFLFAGASALGNGSNPVSGKKPNIIVFLVDDMGWQDTSHPFWSDSQGNPKKTFLNRRYRTPNMEKLASQGMTFTDAYAHPLCTPSRVSLMSGMNPARHRVTCWVREQNGTTDANSRSLLPPDWALNGLQPIGTPARGTTKRPISGEDMRYHMTRPFATAATLPEMLKKCGYVTVHCGKAHFGTQGTPGSNPLNMGFDYNIAGTEIGHPADYRGSRHYGKGFNHVRGLDENNYYQDDVFLTEALTREAIKRLEAIRTNPREAGKPFYLYMAHYALHSPLDERAYDKRFADAYKNPEDGHKWSRTEKHYSGLIEGMDKSLGDIMKYLREHHLEKNTVLVFMSDNGGLAISGRLGNEEANYPLSFGKGSCMEGGIREPMIVSWPGVTKGGSRCAVPVVIDDFFPTLLDIAGCRNVEVPQKLDGLSLVPLLKGGRFPEDRPLLFHQPNNWGEGSRQAPQYTSSTALRQGDWKLIYRHLTQSFELYHLRKDIGEKENLASREPRKTREMAVVMGRLLRERKAQMPTYKKDNELGAPAGSSVPWPDQVKGNGF
>CP024736.1|AYR28771.1|2162619_2163507_+|hypothetical-protein
MRTPSPLPPAPRIKRKRQKTAQNGTSCILFLEMGCIFLFYNVTIAIAMTNIHPFTTAAACILTAGAALFTSSCQPASPESYARQFATAQHVSVHGYNGRETLTSRVYADWGSMPANARRAMEEYIKGAEWKTMDYIRPQYFIQVDRSFWAICIDNDGNMTGIIPFRNGKDARKMSVNNHFKMLVNTTDRAPALGYAILKNLAYADGLRVSTRKADGLETPVPAPPAMKVVVPPKGAAPSAAPAAKSAATSVSEPGATEETPADDTAGTEEEPSADSAAESEDTTTTDDAAVTDDF
>CP024736.1|AYR28770.1|2161336_2162539_-|carboxynorspermidine-decarboxylase
MSAFIISLPALERNALILRDVADAAGCRIALALKGFSCWPCFEALSPWLDGCCASGLWEARLARRRFGKHVLTYSPGHAPEEIAELAEISSHLDFNSLTQWSRFREEVMRHPRFVSGELKCGLRVNPQCSTGHTPIYDPCAPGSRLGVTPDMMQGADLSGISGLHFHTLCEQGADDLAVTMDAVERHFGHLLSRPEISWLNMGGGHWITKPDYDRKLLAALIRRIRERYGVEVWLEPGEATAIHTGVLRCRVLDLFSAEGVNHAILDVSASAHMPDTLEMPYRPDVFQIAGDAFLRSAQQPAVRLEGESYALAGKAGEKAYTYRLGAPTCLAGDRLGDYSFAEPLAVGDELVFDDMAHYTMVKTTFFNGVRHPDIAVQRKDGSVETVRRFSYEDFEARLG
>CP024736.1|AYR29379.1|2159375_2161313_-|biosynthetic-arginine-decarboxylase
MATAKEGIKQKVHGWSHHDSADLYGIDDWGNGYFRISKKGEVTVRLNDEDGGKKDVSLCDLLEGLKERGTTVPVLFRFRDLLRSRIAELNDSFRKAIKEAGYQGKYQGVYPIKVNQQRQVVEEIAEFGTKYDYGLEAGSKPELIAAMAHMHNPNAYLICNGYKDDEFIDLALTAQKMGLNIFIVLEMPSELDVIMERARRMDIRPNLGVRVKLAAKGSGLWQESAGDKSVFGLNAAQVVDVVDKLKQVDALDCLKLLHYHQGSQIPNISVVREGLTEAVRIYVDLVKEGAPLGTLDMGGGLAVDYDGSKTNFHSSCNYSIAEFARDVVEVVGDMCSKYGVPHPTLVTESGRAVVAYYSVLVFNVLDVTSAPGEEPPPELPDDAPELLKAFADTDRTLARKNMQECYNDACYYRDQLRAQFFYGNATLRQRGLGEAYYWHILSRISRMLAEMETIPEDLRELSCSMVDFYYGNFSLFQSLPDSWAIRQLFPVMPLHRLNERPTNKAVLADITCDCDGKIDHFIDREDVATALPLHAIKPGEDDYYIGVFLVGAYQETLGDLHNLLGDTNVVGVHLEGGRPVYTHEVEGDTVSDVLSYVEYDPKELINKFRTFAEQAVAEGKISPSERRRALEVFRSGLNGYTYYEL
>CP024736.1|AYR28769.1|2158204_2159305_+|site-specific-integrase
MNMKKAPNTNKLKYVGAALLEGESVTLIQAARLVLEIKEALGDEICTITRCREVVSLGLNAIKNKHQTVSFGTAAVECLRSKSHRRPRTLTDIRSIIHKLKKSNPELEHTSLRNLSVEECQNILMNTFTTSRQRHKARLILSGIFSFSVKRGWCDENPILRVDTPFLQEQEIPALTLKEITQLLKAAMEEFDGSCAAGAALMIFAGIRPQEVERLLWENIALRDGCIILNSKHTKTGGARHVTILPVLAKWLKFCRDRTKPGPGTPICPKGWTIKWRKIRKKAGWGGRKKSWVPDCLRHTYASYHAKHFKDYNLLQMEMGHRSSSLLRTRYLNMKGISPQTATRFWALTPAKVIEETKPPEEPLVS
>CP024736.1|AYR28768.1|2155746_2157885_-|glycoside-hydrolase-family-2-protein
MNIRIHSFNVSCVGLCCVLGSFFAADAREVYPLNGSWDFSFNGKPAEKVTVPHTWNAEDAANGAQGKREDAKSVNSDAYRRGPAVYSRTLPVEPRPGKRYFIRGGGASIVSEVSVNGKPAGSHEGAFTAFCYEITPLLKKGSNTISVLVDNTQRNHIAPQRGDFSMFGGLYRPIELIETDGVCIDPLFYASPGVFITTKSLSKSKAEVEVKTLLNSDGKAGDVDVAVEILDMKGRKVAGKTVKAAAGEKNRLEVPVTLAIANPILWNGVKNPYLYQVKTSIRTADGQTDELVQPLGLRTVSVNPKEGFVLNGKTMQIKGVSRHQDMKGKGWALSPEDEARDIRLIADMGADGVRTGHYPASTNIYDLCDKAGLIVWSEVPNVNLVWDTPEFRENNRLQAREMIFQHWNHPSICMWGIFNEIGHQPEAATRGVDMEAELTELNKFVKETDPSRMTVGASNQAGRRKLNNIPDHIAFNTYPGWYGGGPETMKGNLNGYIRDYGGKMGIAVSEYGHGASVDMHENPAQRPSPTGFWHPEEWQSHAHEVNYRCIRERPEVWGAFVWNMFDFGSSSRFEGESPGINDKGLVTYDRKTPKDAYFFYRANWSPRPTVYITSRRFAERTEKTVPVKVYSNGSVVKLSVNGKPVGSVKPDDLKRAVWPEVTLKPGVNTITATASIGGKTYTDSCKWTLNPAGGDGKKDSERYQDPSLKKYK
>CP024736.1|AYR29382.1|2172340_2174194_-|biosynthetic-type-acetolactate-synthase-large-subunit
MSTNDKTSSAPTKTRMTGAEVLVECLVREGVDVVFAYPGGASMPIHQALSHQPKIRTILPRHEQGGAFGAEGYGRVTGKVGVCISTSGPGATNMITSIADAYLDSTPLVAITAQVMRSLIGRGAFQETDVFGMTAPIVKHSYLVTEPEELPRIIKEAFYIASTGRPGPVLIDMPKDVQEAVFTPDFDMEMDIPGYNPVLPVPADKLEELIPLIENARRPVIYAGGGIISAEASGDLLEFAERSQIPVATTLMGIGAMPETHPLSLRWLGMHGAVFANNAVNEADLVIALGARFDDRVTGAVNMFCPESTIVHIDIDASEINKNKKVAYPIRANVKDALRVLNSALAAKGWERKSSGYTRTPEWFGVIDGWKKQYPFSYEDRKGYIAPQAVIEELYRQAADKDPIICTGVGQHQMFAAQFFKFDKPRRLATSGGLGTMGYGLPASMGACLAYPDRLVVNIDGDGSMLMNIQELATIHVERLPVKCIILNNQHLGMVVQWEDLKYDSNRAQTFLADPHDNYDPTHKTEDVIYPNYPLICAGFGVKCERVLRIEELPAAITRMIESPEAYVLDVMVPHDVHVLPMILGGMSYKDVILERIAGDGSAKKASELGKEIPTAL
>CP024736.1|AYR28775.1|2174343_2174952_-|beta-phosphoglucomutase-family-hydrolase
MKKRVDLPEKKYEGYIFDLDGTLVDSMPLHYRAWREALARAGAPDHVFRTDEFYSCGGKSANDVVRFLNERYGMHMDAESTAADKRRIYLEMLEKEGMQPIQEVVEFVHSLGDAPKAIATGSAMPGASRTLAAAGLSGLFDVILTPDEVEHGKPAPDMFLKAAELLGASPDRCVVFEDAEPGMKAAAAAGMDCIQVRRPSLA
>CP024736.1|AYR28776.1|2175112_2175376_+|30S-ribosomal-protein-S20
MANTKSALKRIRQTATRTARNRAVTSKLKTLRKKVAAAVETSDKEAAAAAYNTFSSAVDKAAKVGTIPANRAANYKSKAAKAIAKIA
>CP024736.1|AYR28777.1|2175456_2175678_+|hypothetical-protein
MQQSRLDKAKLIIDRPGDFKVCEACGSIVSLTTAVCPNCNAYRFDSNTEYVVEQAVLLASREQRSVTPEDLEI
>CP024736.1|AYR28778.1|2175812_2177012_-|4-hydroxy-3-methylbut-2-enyl-diphosphate-reductase
MNQDSTRKARVNVRRAEVMEQVEKEIQQHYQSELISHIRSAGNVYNLGHTEFFLAREFGFCNGVRRAIDIAYAARRVFPDRRIFLIGDIIHNPEVNRQLEEMGIRKLPWKQLDSSYDRVAPDDVVIIPAFGVPTPFMDALEEKGVQIVDTTCGDVMKVWKRVKNYAAMGITSIIHGKATHEETSATASRALGERGRGKYLVVYDLEDARILCDYIMGRGDREAFLKRFEGCCSPGFDPDRDLEEVGIANQTTMLKTETQTLQKMVKDAIVQRDGDDDNFYVFDTICGATQDRQDALYELLKNPLDVMFVVGGYNSSNTTHLVDIAREHVPTYFIESAECIKSIQYVDAFDTKTREVRRMTTEPVVQNLGKSLKVGITAGASCPANLIEATILRIADLRK
>CP024736.1|AYR28779.1|2177010_2178141_+|tRNA-dihydrouridine-synthase-family-protein
MLPASTLSFFRQDNHSLPTGAFHGMHRPMLLALAPMKDVTDLAFLNTLKDLNSLPDYFITEYFRTVAHHKKMSPYILRSIDENPTGRPIYGQLVGHEPEYLARDAQVLMEHACAGVDLNMGCPAPIVCRRNAGGGMLRSLKAMDAALGALRDVLPPGAFTVKCRLGYETPDEFERILPVIASHSPDRVCIHARTVREGYRSPVHPEWVKWAAGMLKCPVVANGNIVDAATAEAWVRLARPAGLMIGRAALRNPWIFSQLHSRFQGHPAADLTFRNVLHYIRRLYERTREMQEHYVEEKHIHRMKKYLVYTARGLPDTFDHYMKRAKTARDFMRICEDILDNDAPFAPTPPEDTHLFAHFHTLLAQEEACLPPGIQV
>CP024736.1|AYR28780.1|2178153_2179293_+|transglutaminase-domain-containing-protein
MNLLPFALLPGLTAAICAAHDAIQDGVEFLRAHMPAQDRGTVTQERLVREVRLALAARSQFPWAAQVPWELYENNVLPYAVVNEPRDEWREQFHHLFAPLVSPCKTGREAALAIASLIQKTLNVRYSTERRVPHQGVKESLQSGKVSCTGQSILLICALRSVGIPARMAGVLTWNHVRGNHNWVEAWCDGEWKMLEYNEKDFNTPWVMSAISMLDPRKPENAIYATSWKKEPSGAFFPMIWEARYDEKRHALAFPPESRTVPAVNITDRYMKLANEWVVAQPEYVPGSRLMLDIREERKNGARRLPLHVVLKSEEGKVLAEGITPGPSDDMRKFLEVLLPDNISRGMLEFKLPDGTVRHEPVAHTEAPVQILNFFVSAP
>CP024736.1|AYR28781.1|2179289_2180147_+|hypothetical-protein
MKIPLFFNNTARSARAGRFRRWLDRHRDFFDVIEPESREDMLNHLVSQANSGAPAVAVAGGDGTLGIAARALCNTETVLAPFPAGTMNVFSREIGIRPDFDHALHILNTGRSKNVDLFAFNGQVFLQMAGIGADARAVELTTWEMKKKWKALAYVIAGARVCTEKQPHLTLTTDEGRVLEGRSIIFGNGRKYGGPLNFFAEADNSDGLLDAVVFRHSITSIIGEFLMAAVQGGFHSRRSGSFEYIRMTGGTVTSAEQASCELDGDYAGNAPVRITRPGSLKVFVP
>CP024736.1|AYR28782.1|2180288_2181071_-|SDR-family-NAD(P)-dependent-oxidoreductase
MSSKLLEGKVGVVVGVANKRSIAFAIAKAWHEAGAKLIFNYQGERLKDGVLKLIHENFGEDVPVYDLDVSSDESVNAFFERVRQHTDKVDCLLHSVAFAPKEALGGSFLETGREAFKISLDISAYSLVALSRAVEPMMSDNGSILAMSYLGSEKVVPNYNLMGVSKAALEACTRYLAYDLGRSRGIRVNCISAGPMQTLAARGVSGFNTMFKVYEEHAPLGRSCTGEELGATGVFLASDGAAAITGQVIYVDGGYQIMGM
>CP024736.1|AYR28783.1|2181138_2182014_-|bifunctional-5,10-methylene-tetrahydrofolate-dehydrogenase/5,10-methylene-tetrahydrofolate-cyclohydrolase
MQIIDGKQVASQVLEEVERDIARLREQGITPGLAVVLVGEDPASQVYVNSKVKKCAELGMNSRKIVLPSDASQEELLRIVRELNVDPAVHGILVQSPPPPHIDEAAVVLEIDPAKDVDGFHPENVAKLVLEDETGFVPCTPLGCMRLLKAAGIETAGANAVVIGRSMIVGKPMAHLLMSKQANATVTVAHSRTKNLPELCRSADIIVAAIGRPAFVTADFVKDGAVVVDVGINRVEDASAKRGYRIVGDVAYDEVAPKCRAITPVPGGVGPMTIAMLMANTVKACRQQTGA

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity
CP024736_1	1.2|1743976|30|CP024736|CRISPRCasFinder,CRT	1743976-1744005	30	CP024736.1	410410-410439	0	1.0

1. spacer 1.2|1743976|30|CP024736|CRISPRCasFinder,CRT matches to position: 410410-410439, mismatch: 0, identity: 1.0

gcgaagcgggggagtattgcgcacaatgtc	CRISPR spacer
gcgaagcgggggagtattgcgcacaatgtc	Protospacer
******************************

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity
CP024736_1	1.8|1744371|30|CP024736|CRISPRCasFinder,CRT,PILER-CR	1744371-1744400	30	NZ_CP016366	Phaeobacter porticola strain P97 plasmid pP97_b, complete sequence	84898-84927	6	0.8
CP024736_1	1.16|1744899|30|CP024736|CRISPRCasFinder,CRT,PILER-CR	1744899-1744928	30	NZ_AP014802	Rhodovulum sulfidophilum plasmid Plasmid2 DNA, complete genome, strain: DSM 2351	82505-82534	6	0.8
CP024736_1	1.16|1744899|30|CP024736|CRISPRCasFinder,CRT,PILER-CR	1744899-1744928	30	MN428058	Mycobacterium phage Krili, complete genome	58931-58960	6	0.8
CP024736_1	1.16|1744899|30|CP024736|CRISPRCasFinder,CRT,PILER-CR	1744899-1744928	30	MT818418	Mycobacterium phage Shida, complete genome	59043-59072	6	0.8
CP024736_1	1.16|1744899|30|CP024736|CRISPRCasFinder,CRT,PILER-CR	1744899-1744928	30	MH926062	Mycobacterium phage Vorrps, complete genome	59460-59489	6	0.8
CP024736_1	1.16|1744899|30|CP024736|CRISPRCasFinder,CRT,PILER-CR	1744899-1744928	30	NC_022057	Mycobacterium phage Catdawg, complete genome	59422-59451	6	0.8
CP024736_1	1.16|1744899|30|CP024736|CRISPRCasFinder,CRT,PILER-CR	1744899-1744928	30	MT818425	Mycobacterium phage NiebruSaylor, complete genome	58853-58882	6	0.8
CP024736_1	1.20|1745162|30|CP024736|CRISPRCasFinder,CRT,PILER-CR	1745162-1745191	30	NZ_CP050151	Hafnia alvei strain A23BA plasmid pA23BA, complete sequence	48438-48467	7	0.767
CP024736_1	1.20|1745162|30|CP024736|CRISPRCasFinder,CRT,PILER-CR	1745162-1745191	30	NZ_CP029143	Klebsiella michiganensis strain AR375 plasmid unnamed3, complete sequence	6049-6078	7	0.767
CP024736_1	1.20|1745162|30|CP024736|CRISPRCasFinder,CRT,PILER-CR	1745162-1745191	30	NZ_CP013339	Raoultella ornithinolytica strain Yangling I2 plasmid pKPYL1, complete sequence	114636-114665	7	0.767
CP024736_1	1.20|1745162|30|CP024736|CRISPRCasFinder,CRT,PILER-CR	1745162-1745191	30	NZ_CP036439	Klebsiella pneumoniae strain ABFQB plasmid unnamed1, complete sequence	22258-22287	7	0.767
CP024736_1	1.23|1745360|30|CP024736|CRISPRCasFinder,CRT,PILER-CR	1745360-1745389	30	NZ_KM406416	Bifidobacterium breve strain JCM 7017 plasmid megaplasmid pMP7017, complete sequence	137931-137960	7	0.767
CP024736_1	1.24|1745426|30|CP024736|CRISPRCasFinder,CRT,PILER-CR	1745426-1745455	30	MK685668	Shigella phage VB_Ship_A7, complete genome	9525-9554	7	0.767
CP024736_1	1.6|1744240|30|CP024736|CRISPRCasFinder,CRT,PILER-CR	1744240-1744269	30	NZ_CP010826	Thermus aquaticus Y51MC23 plasmid pTA78, complete sequence	29670-29699	8	0.733
CP024736_1	1.16|1744899|30|CP024736|CRISPRCasFinder,CRT,PILER-CR	1744899-1744928	30	NZ_CP017750	Cupriavidus sp. USMAA2-4 plasmid unnamed1, complete sequence	257196-257225	8	0.733
CP024736_1	1.12|1744635|30|CP024736|CRISPRCasFinder,CRT,PILER-CR	1744635-1744664	30	NC_002575	Agrobacterium rhizogenes plasmid pRi1724 DNA, complete sequence	154873-154902	9	0.7
CP024736_1	1.12|1744635|30|CP024736|CRISPRCasFinder,CRT,PILER-CR	1744635-1744664	30	NZ_KY000038	Agrobacterium rhizogenes strain 1724 plasmid pRi_1724, complete sequence	201176-201205	9	0.7
CP024736_1	1.23|1745360|30|CP024736|CRISPRCasFinder,CRT,PILER-CR	1745360-1745389	30	NC_027348	Delftia phage RG-2014, complete genome	14848-14877	9	0.7
CP024736_2	2.1|2171334|34|CP024736|PILER-CR,CRISPRCasFinder,CRT	2171334-2171367	34	NZ_CP017244	Rhizobium etli 8C-3 plasmid pRsp8C3c, complete sequence	758086-758119	9	0.735
CP024736_2	2.7|2171723|34|CP024736|PILER-CR,CRISPRCasFinder,CRT	2171723-2171756	34	MH925093	UNVERIFIED: Vibrio phage VspDsh_1, complete genome	16471-16504	9	0.735
CP024736_2	2.3|2171464|34|CP024736|PILER-CR,CRISPRCasFinder,CRT	2171464-2171497	34	NZ_CP034911	Ensifer alkalisoli strain YIC4027 plasmid unnamed, complete sequence	37356-37389	11	0.676
CP024736_2	2.3|2171464|34|CP024736|PILER-CR,CRISPRCasFinder,CRT	2171464-2171497	34	NZ_CP018236	Rhizobium leguminosarum strain Vaf-108 plasmid unnamed8	72611-72644	11	0.676

1. spacer 1.8|1744371|30|CP024736|CRISPRCasFinder,CRT,PILER-CR matches to NZ_CP016366 (Phaeobacter porticola strain P97 plasmid pP97_b, complete sequence) position: , mismatch: 6, identity: 0.8

gggggcgtcatcgattgtggtggtgatttg	CRISPR spacer
ggcgatgtcatcgcttctggtggtgatttc	Protospacer
** *..******* ** ************ 

2. spacer 1.16|1744899|30|CP024736|CRISPRCasFinder,CRT,PILER-CR matches to NZ_AP014802 (Rhodovulum sulfidophilum plasmid Plasmid2 DNA, complete genome, strain: DSM 2351) position: , mismatch: 6, identity: 0.8

cgaacccggcagacctgctttcccgtattg	CRISPR spacer
atatcccggcagaccggctttcccgcatag	Protospacer
  * *********** *********.** *

3. spacer 1.16|1744899|30|CP024736|CRISPRCasFinder,CRT,PILER-CR matches to MN428058 (Mycobacterium phage Krili, complete genome) position: , mismatch: 6, identity: 0.8

cgaacccggcagacctgctttcccgtattg-	CRISPR spacer
ggaacccgccagacctgcttgcccg-acggc	Protospacer
 ******* *********** **** *. * 

4. spacer 1.16|1744899|30|CP024736|CRISPRCasFinder,CRT,PILER-CR matches to MT818418 (Mycobacterium phage Shida, complete genome) position: , mismatch: 6, identity: 0.8

cgaacccggcagacctgctttcccgtattg-	CRISPR spacer
ggaacccgccagacctgcttgcccg-acggc	Protospacer
 ******* *********** **** *. * 

5. spacer 1.16|1744899|30|CP024736|CRISPRCasFinder,CRT,PILER-CR matches to MH926062 (Mycobacterium phage Vorrps, complete genome) position: , mismatch: 6, identity: 0.8

cgaacccggcagacctgctttcccgtattg-	CRISPR spacer
ggaacccgccagacctgcttgcccg-acggc	Protospacer
 ******* *********** **** *. * 

6. spacer 1.16|1744899|30|CP024736|CRISPRCasFinder,CRT,PILER-CR matches to NC_022057 (Mycobacterium phage Catdawg, complete genome) position: , mismatch: 6, identity: 0.8

cgaacccggcagacctgctttcccgtattg-	CRISPR spacer
ggaacccgccagacctgcttgcccg-acggc	Protospacer
 ******* *********** **** *. * 

7. spacer 1.16|1744899|30|CP024736|CRISPRCasFinder,CRT,PILER-CR matches to MT818425 (Mycobacterium phage NiebruSaylor, complete genome) position: , mismatch: 6, identity: 0.8

cgaacccggcagacctgctttcccgtattg-	CRISPR spacer
ggaacccgccagacctgcttgcccg-acggc	Protospacer
 ******* *********** **** *. * 

8. spacer 1.20|1745162|30|CP024736|CRISPRCasFinder,CRT,PILER-CR matches to NZ_CP050151 (Hafnia alvei strain A23BA plasmid pA23BA, complete sequence) position: , mismatch: 7, identity: 0.767

gtatccttaaactcttcaatggtcttcggg	CRISPR spacer
tcatcctctaactcttcaatggtcttgagc	Protospacer
 .*****. ***************** .* 

9. spacer 1.20|1745162|30|CP024736|CRISPRCasFinder,CRT,PILER-CR matches to NZ_CP029143 (Klebsiella michiganensis strain AR375 plasmid unnamed3, complete sequence) position: , mismatch: 7, identity: 0.767

gtatccttaaactcttcaatggtcttcggg	CRISPR spacer
gtggtgttaatctcttcaatggtctgcggt	Protospacer
**. . **** ************** *** 

10. spacer 1.20|1745162|30|CP024736|CRISPRCasFinder,CRT,PILER-CR matches to NZ_CP013339 (Raoultella ornithinolytica strain Yangling I2 plasmid pKPYL1, complete sequence) position: , mismatch: 7, identity: 0.767

gtatccttaaactcttcaatggtcttcggg	CRISPR spacer
gtggtgttaatctcttcaatggtctgcggt	Protospacer
**. . **** ************** *** 

11. spacer 1.20|1745162|30|CP024736|CRISPRCasFinder,CRT,PILER-CR matches to NZ_CP036439 (Klebsiella pneumoniae strain ABFQB plasmid unnamed1, complete sequence) position: , mismatch: 7, identity: 0.767

gtatccttaaactcttcaatggtcttcggg	CRISPR spacer
gtggtgttaatctcttcaatggtctgcggt	Protospacer
**. . **** ************** *** 

12. spacer 1.23|1745360|30|CP024736|CRISPRCasFinder,CRT,PILER-CR matches to NZ_KM406416 (Bifidobacterium breve strain JCM 7017 plasmid megaplasmid pMP7017, complete sequence) position: , mismatch: 7, identity: 0.767

tatttgc-----cgaaacggtatctgacgcccgcc	CRISPR spacer
-----gccgacgcgaaacggtatctgccgcccgac	Protospacer
     **     ************** ****** *

13. spacer 1.24|1745426|30|CP024736|CRISPRCasFinder,CRT,PILER-CR matches to MK685668 (Shigella phage VB_Ship_A7, complete genome) position: , mismatch: 7, identity: 0.767

aaggggttgtaggtcaactggtccacaatg	CRISPR spacer
agtgggatgtaggtcaacgggtccacacca	Protospacer
*. *** *********** ******** ..

14. spacer 1.6|1744240|30|CP024736|CRISPRCasFinder,CRT,PILER-CR matches to NZ_CP010826 (Thermus aquaticus Y51MC23 plasmid pTA78, complete sequence) position: , mismatch: 8, identity: 0.733

ccggggaatccgggggtgccaagcctgttt	CRISPR spacer
ccggggaatccgggagtgccagcccatgac	Protospacer
**************.******. **    .

15. spacer 1.16|1744899|30|CP024736|CRISPRCasFinder,CRT,PILER-CR matches to NZ_CP017750 (Cupriavidus sp. USMAA2-4 plasmid unnamed1, complete sequence) position: , mismatch: 8, identity: 0.733

cgaacccggcagacctgctttcccgtattg	CRISPR spacer
tctacccggcagacgtgccttcccgtctcc	Protospacer
.  *********** ***.******* *. 

16. spacer 1.12|1744635|30|CP024736|CRISPRCasFinder,CRT,PILER-CR matches to NC_002575 (Agrobacterium rhizogenes plasmid pRi1724 DNA, complete sequence) position: , mismatch: 9, identity: 0.7

tcgtgagagccgccgccgctaagttaagag	CRISPR spacer
tcgtgagagccgcggccgccaagcaggcga	Protospacer
************* *****.***. .. ..

17. spacer 1.12|1744635|30|CP024736|CRISPRCasFinder,CRT,PILER-CR matches to NZ_KY000038 (Agrobacterium rhizogenes strain 1724 plasmid pRi_1724, complete sequence) position: , mismatch: 9, identity: 0.7

tcgtgagagccgccgccgctaagttaagag	CRISPR spacer
tcgtgagagccgcggccgccaagcaggcga	Protospacer
************* *****.***. .. ..

18. spacer 1.23|1745360|30|CP024736|CRISPRCasFinder,CRT,PILER-CR matches to NC_027348 (Delftia phage RG-2014, complete genome) position: , mismatch: 9, identity: 0.7

tatttgccgaaacggtatctgacgcccgcc	CRISPR spacer
cccatgccgaagcggtagctgacgcccagg	Protospacer
. . *******.***** *********.  

19. spacer 2.1|2171334|34|CP024736|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP017244 (Rhizobium etli 8C-3 plasmid pRsp8C3c, complete sequence) position: , mismatch: 9, identity: 0.735

tcaaggcgattgattgaccagttcggcaagtagt	CRISPR spacer
gcttggcgaaagattgaccagttcggcaagctcg	Protospacer
 *  *****  *******************.   

20. spacer 2.7|2171723|34|CP024736|PILER-CR,CRISPRCasFinder,CRT matches to MH925093 (UNVERIFIED: Vibrio phage VspDsh_1, complete genome) position: , mismatch: 9, identity: 0.735

cgtttccgggttttcgattatgcgcactaggccc	CRISPR spacer
cgtttcagggttttcgattaagcgtagctgagct	Protospacer
****** ************* ***.* . *. *.

21. spacer 2.3|2171464|34|CP024736|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP034911 (Ensifer alkalisoli strain YIC4027 plasmid unnamed, complete sequence) position: , mismatch: 11, identity: 0.676

agccaaccgcttatgacgttctcgcttgcctgac	CRISPR spacer
agccaacctcttatgacgttcacgtcgacgccgg	Protospacer
******** ************ **.. .* . . 

22. spacer 2.3|2171464|34|CP024736|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP018236 (Rhizobium leguminosarum strain Vaf-108 plasmid unnamed8) position: , mismatch: 11, identity: 0.676

agccaaccgcttatgacgttctcgcttgcctgac	CRISPR spacer
agccaacctcttatgacgttcacgtcgacaccgg	Protospacer
******** ************ **.. .* . .