CRISPRimmunity

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Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Prophage number
CP033741	Enterococcus sp. FDAARGOS_553 plasmid unnamed2, complete sequence	0 crisprs	csa3	0
CP033740	Enterococcus sp. FDAARGOS_553 chromosome, complete genome	0 crisprs	cas3,csa3,cas14j,DEDDh,DinG	4
CP033739	Enterococcus sp. FDAARGOS_553 plasmid unnamed1, complete sequence	0 crisprs	NA	2

Results visualization

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CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

462757 : 505658

Bacillus_virus(16.67%)

transposase,tail,integrase

attL 487553:487574|attR 505754:505775

The bacterium proteins that are colored denote the protein is present at specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin' and 'tRNA')

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYY08823.1\|462757_463948_-\|transposase	IS256-like element ISLgar5 family transposase	NA	NA	NA	NA
AYY08824.1\|464245_465592_-	aspartate ammonia-lyase	NA	NA	NA	NA
AYY08825.1\|465750_466182_+	Lrp/AsnC family transcriptional regulator	NA	NA	NA	NA
AYY08826.1\|466240_466687_-	Lrp/AsnC family transcriptional regulator	NA	NA	NA	NA
AYY08827.1\|466779_467952_-	MFS transporter	NA	NA	NA	NA
AYY08828.1\|467966_468719_-	amino acid ABC transporter ATP-binding protein	G3M9Y6	Bacillus_virus	4.5e-19	29.8
AYY08829.1\|468744_469716_-	D-2-hydroxyacid dehydrogenase	M1I636	Acanthocystis_turfacea_Chlorella_virus	9.8e-35	32.1
AYY08830.1\|469728_470949_-	PLP-dependent aminotransferase family protein	NA	NA	NA	NA
AYY08831.1\|470972_471719_-	amino acid ABC transporter ATP-binding protein	G9BWD6	Planktothrix_phage	5.0e-31	37.3
AYY08832.1\|471715_472426_-	amino acid ABC transporter permease	NA	NA	NA	NA
AYY08833.1\|472438_473251_-	amino acid ABC transporter	NA	NA	NA	NA
AYY08834.1\|473286_474039_-	amino acid ABC transporter permease	NA	NA	NA	NA
AYY08835.1\|474285_475029_+	hypothetical protein	NA	NA	NA	NA
AYY08836.1\|475065_475563_-	GNAT family N-acetyltransferase	NA	NA	NA	NA
AYY08837.1\|476930_477188_-	MerR family transcriptional regulator	NA	NA	NA	NA
AYY08838.1\|477609_477849_+	hypothetical protein	NA	NA	NA	NA
AYY08839.1\|478254_479019_-	iron export ABC transporter permease subunit FetB	NA	NA	NA	NA
AYY08840.1\|479015_479669_-	ATP-binding cassette domain-containing protein	G3M9Y6	Bacillus_virus	3.1e-24	36.4
AYY08841.1\|479752_480175_-	cupin domain-containing protein	Q2I8C5	Bacillus_phage	2.4e-38	58.5
AYY08842.1\|481082_481577_+	ATP-binding protein	NA	NA	NA	NA
AYY08843.1\|481956_482313_-	XRE family transcriptional regulator	NA	NA	NA	NA
AYY08844.1\|482510_482594_+	type I toxin-antitoxin system Fst family toxin	NA	NA	NA	NA
AYY08845.1\|482908_483109_-	LPXTG cell wall anchor domain-containing protein	NA	NA	NA	NA
AYY08846.1\|486142_487438_+\|transposase	ISL3-like element ISEfa5 family transposase	A0A2D1GQC1	Lysinibacillus_phage	6.5e-10	21.4
487553:487574	attL	NA	NA	NA	NA
AYY08847.1\|488268_488505_-	hypothetical protein	NA	NA	NA	NA
AYY08848.1\|488491_489121_-	hypothetical protein	NA	NA	NA	NA
AYY08849.1\|489167_489497_-	DUF771 domain-containing protein	U3PBP3	Staphylococcus_phage	3.4e-08	34.4
AYY08850.1\|489534_489831_-	hypothetical protein	NA	NA	NA	NA
AYY11193.1\|489951_490656_-\|tail	phage tail protein	A0A249XXB4	Clostridium_phage	1.2e-21	54.4
AYY08851.1\|490903_491122_-	hypothetical protein	NA	NA	NA	NA
AYY08852.1\|491122_491341_-	XRE family transcriptional regulator	NA	NA	NA	NA
AYY08853.1\|491477_492119_+	XRE family transcriptional regulator	NA	NA	NA	NA
AYY08854.1\|492170_493313_+\|integrase	site-specific integrase	Q938N9	Temperate_phage	4.1e-56	35.4
AYY08855.1\|494422_494782_+	hypothetical protein	NA	NA	NA	NA
AYY08856.1\|494778_495462_+	helix-turn-helix domain-containing protein	NA	NA	NA	NA
AYY08857.1\|495419_495818_+	hypothetical protein	NA	NA	NA	NA
AYY08858.1\|496050_496776_-\|tail	phage tail protein	A0A249XXB4	Clostridium_phage	2.4e-14	51.3
AYY08859.1\|497214_497499_-	hypothetical protein	NA	NA	NA	NA
AYY08860.1\|497482_497755_-	hypothetical protein	NA	NA	NA	NA
AYY08861.1\|497777_498377_-	hypothetical protein	NA	NA	NA	NA
AYY08862.1\|498376_498835_-	hypothetical protein	NA	NA	NA	NA
AYY08863.1\|499279_499609_-	hypothetical protein	NA	NA	NA	NA
AYY11194.1\|499630_499891_-	hypothetical protein	NA	NA	NA	NA
AYY08864.1\|501589_502495_-	hypothetical protein	D2J048	Enterococcus_phage	1.5e-05	38.8
AYY08865.1\|502491_502752_-	hypothetical protein	NA	NA	NA	NA
AYY08866.1\|502882_503128_-	hypothetical protein	NA	NA	NA	NA
AYY08867.1\|503203_503476_-	hypothetical protein	NA	NA	NA	NA
AYY08868.1\|503631_504465_+	XRE family transcriptional regulator	NA	NA	NA	NA
AYY08869.1\|504506_505658_+\|integrase	site-specific integrase	A0A1S5S7K9	Streptococcus_phage	1.3e-54	33.3
505754:505775	attR	NA	NA	NA	NA

DBSCAN-SWA_2

1404329 : 1413858

Only_Syngen_Nebraska_virus(33.33%)

transposase,tRNA

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYY09660.1\|1404329_1405376_+	ABC transporter ATP-binding protein	G9BWD6	Planktothrix_phage	3.3e-20	30.9
AYY09661.1\|1405390_1406326_+	ABC transporter ATP-binding protein	A0A1J0FA64	Only_Syngen_Nebraska_virus	2.7e-05	23.5
AYY09662.1\|1406342_1407380_+\|transposase	transposase	A0A288TXV8	Enterococcus_phage	1.7e-61	45.1
AYY09663.1\|1407578_1408409_-	NUDIX hydrolase	G3MA14	Bacillus_virus	2.7e-25	31.7
AYY09664.1\|1408454_1408745_-	hypothetical protein	NA	NA	NA	NA
AYY09665.1\|1408908_1410915_+\|tRNA	methionine--tRNA ligase	A0A1V0SHR2	Klosneuvirus	1.9e-96	37.7
AYY09666.1\|1411179_1413858_+	cation-translocating P-type ATPase	A0A1J0FA34	Only_Syngen_Nebraska_virus	1.4e-70	28.2

DBSCAN-SWA_3

2412880 : 2421934

Lysinibacillus_phage(16.67%)

transposase

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYY10536.1\|2412880_2414176_-\|transposase	ISL3-like element ISEfa5 family transposase	A0A2D1GQC1	Lysinibacillus_phage	6.5e-10	21.4
AYY10537.1\|2414359_2414746_-	hypothetical protein	NA	NA	NA	NA
AYY10538.1\|2415010_2415733_+	phosphoribosylaminoimidazolesuccinocarboxamide synthase	A0A1D7SEW9	Cyanophage	1.8e-46	41.7
AYY10539.1\|2415723_2415987_+	phosphoribosylformylglycinamidine synthase subunit PurS	NA	NA	NA	NA
AYY10540.1\|2415983_2416661_+	phosphoribosylformylglycinamidine synthase subunit PurQ	NA	NA	NA	NA
AYY10541.1\|2416653_2418882_+	phosphoribosylformylglycinamidine synthase subunit PurL	A6N228	Microbacterium_phage	3.5e-152	41.3
AYY10542.1\|2418866_2420288_+	amidophosphoribosyltransferase	A0A0M3SGR2	Mollivirus	7.3e-55	34.5
AYY10543.1\|2420309_2421356_+	phosphoribosylformylglycinamidine cyclo-ligase	Q58MH8	Prochlorococcus_phage	2.9e-61	42.6
AYY10544.1\|2421352_2421934_+	phosphoribosylglycinamide formyltransferase	M4QRX9	Synechococcus_phage	1.3e-26	38.5

DBSCAN-SWA_4

2697587 : 2706477

Lactobacillus_phage(42.86%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYY10789.1\|2697587_2700011_-	flavocytochrome c	A0A2P0ZL82	Lactobacillus_phage	0.0e+00	66.5
AYY10790.1\|2700029_2700635_-	NAD(P)H-dependent oxidoreductase	A0A2P0ZL77	Lactobacillus_phage	7.4e-73	68.2
AYY10791.1\|2700934_2701849_+	LysR family transcriptional regulator	A0A2P0ZL89	Lactobacillus_phage	3.0e-70	44.7
AYY10792.1\|2702091_2703042_-	FAD:protein FMN transferase	NA	NA	NA	NA
AYY10793.1\|2703156_2703618_-	6,7-dimethyl-8-ribityllumazine synthase	A0A2H4PQS3	Staphylococcus_phage	2.9e-37	54.0
AYY10794.1\|2703637_2704816_-	bifunctional 3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP cyclohydrolase II	A0A2H4PQS2	Staphylococcus_phage	5.6e-101	50.4
AYY10795.1\|2704808_2705429_-	riboflavin synthase	A0A2H4PQS5	Staphylococcus_phage	2.5e-31	38.9
AYY10796.1\|2705397_2706477_-	bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD	A0A1V0SE20	Indivirus	2.0e-36	35.3

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

2. CP033739

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

3542 : 36024

Streptococcus_phage(50.0%)

bacteriocin,integrase,transposase

attL 10753:10767|attR 42332:42346

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYY08359.1\|3542_4223_+\|transposase	IS6 family transposase	A0A1X9I6C6	Streptococcus_phage	2.6e-119	90.7
AYY08360.1\|4399_5446_-	LacI family transcriptional regulator	C6ZCU4	Enterobacteria_phage	3.1e-26	28.1
AYY08361.1\|5779_6094_-	PTS lactose/cellobiose transporter subunit IIA	NA	NA	NA	NA
AYY08362.1\|6170_7514_-	PTS sugar transporter subunit IIC	NA	NA	NA	NA
AYY08363.1\|7532_7835_-	PTS sugar transporter subunit IIB	NA	NA	NA	NA
AYY08364.1\|7882_9322_-	glycoside hydrolase family 1 protein	A0A0B5JD41	Pandoravirus	1.3e-51	31.5
AYY08365.1\|9395_10289_-	ROK family protein	NA	NA	NA	NA
10753:10767	attL	NA	NA	NA	NA
AYY08366.1\|11516_12203_+\|transposase	IS6-like element ISEnfa1 family transposase	A0A1X9I6C6	Streptococcus_phage	2.4e-112	88.1
AYY08367.1\|12798_13281_+	hypothetical protein	NA	NA	NA	NA
AYY08368.1\|13302_13560_+	hypothetical protein	NA	NA	NA	NA
AYY08369.1\|13677_14016_-	hypothetical protein	NA	NA	NA	NA
AYY08370.1\|14042_14612_-	ArsR family transcriptional regulator	NA	NA	NA	NA
AYY08371.1\|14867_14954_+	type I toxin-antitoxin system Fst family toxin	NA	NA	NA	NA
AYY08372.1\|15133_15406_-	hypothetical protein	NA	NA	NA	NA
AYY08373.1\|15709_16171_-	hypothetical protein	NA	NA	NA	NA
AYY08374.1\|16195_16561_-	hypothetical protein	NA	NA	NA	NA
AYY08375.1\|16958_17645_+\|transposase	IS6-like element ISEnfa1 family transposase	A0A1X9I6C6	Streptococcus_phage	2.4e-112	88.1
AYY08376.1\|17959_19639_-	D-lactate dehydrogenase	NA	NA	NA	NA
AYY08377.1\|20786_21074_-	type III secretion system protein PrgN	NA	NA	NA	NA
AYY08378.1\|21138_21819_+\|transposase	IS6 family transposase	A0A1X9I6C6	Streptococcus_phage	2.2e-118	89.8
AYY08379.1\|21906_22191_-	type III secretion system protein PrgN	NA	NA	NA	NA
AYY08380.1\|22348_23842_-	replication protein RepR	NA	NA	NA	NA
AYY08381.1\|24225_24552_-	XRE family transcriptional regulator	NA	NA	NA	NA
AYY08382.1\|24938_25904_+	ParA family protein	NA	NA	NA	NA
AYY08383.1\|25903_26368_+	hypothetical protein	NA	NA	NA	NA
AYY08384.1\|26740_28117_+	Y-family DNA polymerase	M1Q231	Streptococcus_phage	7.7e-94	41.9
AYY08385.1\|28100_28604_+	hypothetical protein	NA	NA	NA	NA
AYY08386.1\|28697_29288_+	DUF159 family protein	A0A1P8CX02	Bacillus_phage	1.3e-10	34.6
AYY08387.1\|29372_29945_-	hypothetical protein	NA	NA	NA	NA
AYY08388.1\|30076_30265_-	hypothetical protein	NA	NA	NA	NA
AYY08389.1\|30728_30830_-	hypothetical protein	NA	NA	NA	NA
AYY08390.1\|31613_32621_-	Abi family protein	A3QSC6	Clostridium_virus	4.4e-22	26.8
AYY08391.1\|33371_33560_-	hypothetical protein	NA	NA	NA	NA
AYY08392.1\|33677_34382_-	hypothetical protein	NA	NA	NA	NA
AYY08393.1\|34430_35030_-\|integrase	site-specific integrase	D2XR58	Bacillus_phage	1.9e-28	35.6
AYY08394.1\|35727_36024_-\|bacteriocin	bacteriocin immunity protein	NA	NA	NA	NA
42332:42346	attR	NA	NA	NA	NA

DBSCAN-SWA_2

54635 : 59630

Streptococcus_phage(100.0%)

integrase

attL 52075:52088|attR 60296:60309

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
52075:52088	attL	NA	NA	NA	NA
AYY08412.1\|54635_54920_-	antitoxin	A0A1X9I6E5	Streptococcus_phage	5.6e-07	96.7
AYY08413.1\|54936_55152_-	peptide-binding protein	A0A1X9I6D5	Streptococcus_phage	4.2e-31	97.1
AYY08414.1\|55225_55795_-	ParA family protein	A0A1X9I765	Streptococcus_phage	8.1e-98	95.8
AYY08415.1\|56199_56478_-	topoisomerase	A0A1X9I6W8	Streptococcus_phage	6.6e-37	92.6
AYY08416.1\|56583_57429_-	AraC family transcriptional regulator	A0A1B0RXG1	Streptococcus_phage	1.3e-22	42.9
AYY08417.1\|57583_57976_-	recombinase family protein	NA	NA	NA	NA
AYY08418.1\|58154_59348_-\|integrase	site-specific integrase	A0A1S5SEW7	Streptococcus_phage	2.4e-144	64.1
AYY08419.1\|59426_59630_-	excisionase	A0A1S5SF07	Streptococcus_phage	6.6e-26	82.1
60296:60309	attR	NA	NA	NA	NA

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

Overview of predicted results

Overview of the results

Cas Category Instructions

Results visualization

1. CP033740

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection

2. CP033739

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection