CRISPRimmunity

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Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Prophage number
CP033749	Burkholderia multivorans strain FDAARGOS_547 chromosome 2, complete sequence	0 crisprs	csa3	0
CP033750	Burkholderia multivorans strain FDAARGOS_547 chromosome 3, complete sequence	0 crisprs	WYL,cas3,DEDDh,csa3,DinG	4
CP033748	Burkholderia multivorans strain FDAARGOS_547 chromosome 1, complete sequence	0 crisprs	csa3,cas3,DinG	0

Results visualization

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CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

261480 : 270452

Hokovirus(16.67%)

The bacterium proteins that are colored denote the protein is present at specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin' and 'tRNA')

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYY58124.1\|261480_263427_+	molecular chaperone DnaK	A0A1V0SH73	Hokovirus	2.7e-148	51.2
AYY58125.1\|263691_264822_+	molecular chaperone DnaJ	A0A1V0SBY2	Catovirus	2.6e-23	30.0
AYY58126.1\|264825_266688_+	aminodeoxychorismate synthase component I	S4VT78	Pandoravirus	2.2e-59	38.2
AYY58127.1\|266793_267609_-	3-methyl-2-oxobutanoate hydroxymethyltransferase	A0A0N7HVJ8	Ostreococcus_lucimarinus_virus	3.9e-37	33.7
AYY58128.1\|267655_268342_-	deoxynucleoside kinase	A0A0M3UL55	Enterococcus_phage	2.7e-07	30.1
AYY58129.1\|268338_268878_-	2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase	NA	NA	NA	NA
AYY58130.1\|268913_270452_-	polynucleotide adenylyltransferase PcnB	H7BUW3	unidentified_phage	4.7e-23	30.7

DBSCAN-SWA_2

596000 : 604279

Bacillus_phage(16.67%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYY58406.1\|596000_597401_+	UDP-glucose/GDP-mannose dehydrogenase family protein	A0A127AXI2	Bacillus_phage	2.3e-77	37.7
AYY60811.1\|597408_598359_+	D-glycero-beta-D-manno-heptose-7-phosphate kinase	A0A2H4N7X4	Lake_Baikal_phage	1.2e-16	27.5
AYY58407.1\|598407_599400_+	ADP-L-glycero-D-mannoheptose-6-epimerase	R9S880	Prochlorococcus_phage	6.9e-28	30.9
AYY58408.1\|599475_599847_+	helix-hairpin-helix domain-containing protein	NA	NA	NA	NA
AYY58409.1\|600056_600959_+	cysteine synthase CysM	A0A1X9I5F1	Streptococcus_phage	4.8e-52	39.5
AYY60812.1\|601039_602380_-	lytic murein transglycosylase B	NA	NA	NA	NA
AYY58410.1\|602426_603350_+	histone deacetylase family protein	A0A2K9KZC4	Tupanvirus	2.3e-41	34.0
AYY58411.1\|603370_604279_-	lysophospholipase	Q6TUZ7	Yaba_monkey_tumor_virus	6.2e-15	26.4

DBSCAN-SWA_3

2267065 : 2371648

109

Burkholderia_phage(72.73%)

integrase,capsid,portal,terminase,holin,plate,protease,head,tail

attL 2300407:2300425|attR 2353625:2353643

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYY59841.1\|2267065_2268550_-\|protease	DegQ family serine endoprotease	A0A1B1IT49	uncultured_Mediterranean_phage	8.8e-27	30.6
AYY59842.1\|2268648_2269995_-	two-component sensor histidine kinase	W8CYF6	Bacillus_phage	8.8e-18	29.5
AYY60905.1\|2269998_2270661_-	response regulator	NA	NA	NA	NA
AYY60906.1\|2270836_2272369_+	D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase	NA	NA	NA	NA
AYY59843.1\|2272573_2273488_+	sterol desaturase family protein	NA	NA	NA	NA
AYY59844.1\|2273575_2274706_+	acylhydrolase	NA	NA	NA	NA
AYY59845.1\|2274825_2275854_-	threonylcarbamoyl-AMP synthase	S4VW33	Pandoravirus	2.2e-40	38.2
AYY59846.1\|2275865_2277062_-	5-(carboxyamino)imidazole ribonucleotide synthase	NA	NA	NA	NA
AYY60907.1\|2277147_2277669_-	5-(carboxyamino)imidazole ribonucleotide mutase	NA	NA	NA	NA
AYY59847.1\|2277685_2278576_-	phosphoribosylaminoimidazolesuccinocarboxamide synthase	A0A0M4JBD3	Mollivirus	5.4e-56	39.7
AYY59848.1\|2278794_2279859_-	fructose-bisphosphate aldolase class II	NA	NA	NA	NA
AYY59849.1\|2280157_2281594_-	pyruvate kinase	NA	NA	NA	NA
AYY59850.1\|2281879_2283076_-	phosphoglycerate kinase	NA	NA	NA	NA
AYY59851.1\|2283067_2283460_+	hypothetical protein	NA	NA	NA	NA
AYY59852.1\|2283354_2283678_-	AzlD domain-containing protein	NA	NA	NA	NA
AYY59853.1\|2283674_2284430_-	branched-chain amino acid ABC transporter permease	NA	NA	NA	NA
AYY59854.1\|2284621_2285545_+	branched-chain amino acid transaminase	NA	NA	NA	NA
AYY59855.1\|2285595_2285790_+	zinc-finger domain-containing protein	NA	NA	NA	NA
AYY59856.1\|2285929_2286967_+	lipopolysaccharide heptosyltransferase II	NA	NA	NA	NA
AYY59857.1\|2287082_2287529_+	hypothetical protein	NA	NA	NA	NA
AYY59858.1\|2287556_2288597_+	hydrolase	NA	NA	NA	NA
AYY59859.1\|2288589_2289186_+	DUF2946 family protein	NA	NA	NA	NA
AYY59860.1\|2289278_2290985_-	M48 family peptidase	NA	NA	NA	NA
AYY59861.1\|2291310_2291790_+	cyclic pyranopterin monophosphate synthase MoaC	NA	NA	NA	NA
AYY59862.1\|2291928_2292912_+\|integrase	site-specific integrase	A0A1L7DQ84	Ralstonia_phage	1.1e-49	38.5
AYY59863.1\|2293599_2293938_-	hypothetical protein	NA	NA	NA	NA
AYY59864.1\|2293991_2294987_-	restriction endonuclease	NA	NA	NA	NA
AYY59865.1\|2295270_2295969_-	hypothetical protein	NA	NA	NA	NA
AYY59866.1\|2296030_2296774_-	HNH endonuclease	NA	NA	NA	NA
AYY59867.1\|2296964_2297876_-	hypothetical protein	NA	NA	NA	NA
AYY59868.1\|2298554_2298905_-	TonB family protein	NA	NA	NA	NA
AYY59869.1\|2299495_2301277_-	polymerase	NA	NA	NA	NA
2300407:2300425	attL	NA	NA	NA	NA
AYY59870.1\|2301400_2302000_-	pilin	NA	NA	NA	NA
AYY59871.1\|2302101_2302806_-	TerC family protein	A0A0S4KZH7	Pseudomonas_phage	5.3e-38	46.1
AYY59872.1\|2302962_2303844_-	succinate--CoA ligase subunit alpha	NA	NA	NA	NA
AYY59873.1\|2303991_2305158_-	succinyl-CoA ligase subunit beta	NA	NA	NA	NA
AYY59874.1\|2305226_2305826_-	DUF2889 domain-containing protein	NA	NA	NA	NA
AYY59875.1\|2305972_2306830_-	recombination regulator RecX	NA	NA	NA	NA
AYY59876.1\|2306829_2307903_-	recombinase RecA	A0A0S2MVG1	Bacillus_phage	3.3e-116	65.5
AYY59877.1\|2308091_2308850_+	DNA-binding response regulator	NA	NA	NA	NA
AYY59878.1\|2308868_2310425_+	sensor histidine kinase	NA	NA	NA	NA
AYY59879.1\|2310651_2312310_+	MFS transporter	NA	NA	NA	NA
AYY59880.1\|2312726_2313245_+	PAAR domain-containing protein	A4JWV5	Burkholderia_virus	2.1e-60	66.3
AYY59881.1\|2313241_2313973_+	VRR-NUC domain-containing protein	A4JWV4	Burkholderia_virus	1.5e-48	49.1
AYY59882.1\|2313982_2315092_+	DUF3396 domain-containing protein	E5E3X7	Burkholderia_phage	1.8e-189	87.2
AYY59883.1\|2315478_2315664_+	hypothetical protein	NA	NA	NA	NA
AYY59884.1\|2315666_2318261_+	SAM-dependent DNA methyltransferase	A0A142K7G3	Mycobacterium_phage	1.5e-13	20.7
AYY59885.1\|2318747_2319800_-\|portal	phage portal protein	E5E3S7	Burkholderia_phage	3.3e-201	98.0
AYY59886.1\|2319799_2321551_-	oxidoreductase	E5E3S6	Burkholderia_phage	0.0e+00	95.6
AYY59887.1\|2321700_2322522_+\|capsid	GPO family capsid scaffolding protein	E5E3S5	Burkholderia_phage	8.9e-146	99.6
AYY59888.1\|2322558_2323584_+\|capsid	phage major capsid protein, P2 family	E5E3S4	Burkholderia_phage	3.5e-192	99.4
AYY59889.1\|2323580_2324267_+\|terminase	terminase	E5E3S3	Burkholderia_phage	2.6e-122	97.4
AYY59890.1\|2324371_2324848_+\|head	head completion/stabilization protein	E5E3S2	Burkholderia_phage	3.4e-81	97.5
AYY59891.1\|2324847_2325090_+	hypothetical protein	E5E3S1	Burkholderia_phage	4.6e-34	95.0
AYY59892.1\|2325089_2325302_+\|tail	phage tail protein	E5E3S0	Burkholderia_phage	5.2e-34	98.6
AYY59893.1\|2325304_2325679_+	hypothetical protein	E5E3R9	Burkholderia_phage	6.0e-57	98.4
AYY59894.1\|2325678_2325999_+\|holin	phage holin family protein	E5E3R8	Burkholderia_phage	2.3e-49	99.1
AYY59895.1\|2325991_2326792_+	DUF3380 domain-containing protein	E5E3R7	Burkholderia_phage	2.9e-133	91.7
AYY59896.1\|2326788_2327280_+	hypothetical protein	A0A1S5NQ26	Burkholderia_phage	9.9e-68	86.5
AYY59897.1\|2327276_2327687_+\|tail	phage tail protein	E5E3R4	Burkholderia_phage	1.2e-71	96.3
AYY59898.1\|2327686_2328136_+	phage virion morphogenesis protein	E5E3R3	Burkholderia_phage	2.1e-64	89.9
AYY59899.1\|2328553_2329186_+\|plate	phage baseplate assembly protein V	E5E3R1	Burkholderia_phage	2.3e-117	99.0
AYY59900.1\|2329182_2329560_+	hypothetical protein	E5E3R0	Burkholderia_phage	2.3e-64	98.4
AYY59901.1\|2329556_2330462_+\|plate	baseplate assembly protein	A0A1S5NR72	Burkholderia_phage	8.3e-153	92.7
AYY59902.1\|2330454_2331009_+\|tail	phage tail protein I	E5E3Q8	Burkholderia_phage	7.7e-93	91.8
AYY59903.1\|2331011_2332622_+\|tail	phage tail protein	E5E3Q7	Burkholderia_phage	7.3e-253	95.1
AYY59904.1\|2332632_2333484_+\|tail	phage tail protein	E5E3Q6	Burkholderia_phage	1.5e-148	89.4
AYY59905.1\|2333631_2333814_+	Com family DNA-binding transcriptional regulator	E5E3Q5	Burkholderia_phage	9.1e-27	95.0
AYY59906.1\|2333791_2334541_+	site-specific DNA-methyltransferase	E5E3Q4	Burkholderia_phage	3.3e-139	96.0
AYY59907.1\|2334652_2335825_+\|tail	phage tail sheath protein	E5E3Q3	Burkholderia_phage	9.5e-226	99.0
AYY59908.1\|2335854_2336364_+\|tail	phage major tail tube protein	E5E3Q2	Burkholderia_phage	1.6e-92	99.4
AYY59909.1\|2336396_2336708_+\|tail	phage tail assembly protein	E5E3Q1	Burkholderia_phage	4.6e-47	100.0
AYY59910.1\|2336704_2336827_+\|tail	GpE family phage tail protein	E5E3Q0	Burkholderia_phage	5.3e-15	97.5
AYY59911.1\|2336823_2340075_+	hypothetical protein	A0A1S5NRM8	Burkholderia_phage	0.0e+00	78.7
AYY59912.1\|2340088_2340517_+\|tail	phage tail protein	E5E3P8	Burkholderia_phage	7.3e-75	100.0
AYY59913.1\|2340513_2341662_+	phage late control D family protein	E5E3P7	Burkholderia_phage	1.4e-197	99.5
AYY59914.1\|2342128_2342509_+	LysR family transcriptional regulator	NA	NA	NA	NA
AYY59915.1\|2342514_2343006_-	XRE family transcriptional regulator	E5E3U2	Burkholderia_phage	1.3e-24	42.1
AYY60908.1\|2343123_2343309_+	hypothetical protein	A4JWR7	Burkholderia_virus	2.0e-05	54.0
AYY60909.1\|2343324_2343519_+	hypothetical protein	E5E3U0	Burkholderia_phage	2.7e-13	58.7
AYY59916.1\|2343544_2343736_+	hypothetical protein	E5E3T9	Burkholderia_phage	1.2e-10	57.8
AYY59917.1\|2343763_2344012_+	transcriptional regulator	E5E3P1	Burkholderia_phage	1.0e-36	89.0
AYY59918.1\|2344101_2344296_+	hypothetical protein	E5E3P0	Burkholderia_phage	1.5e-27	93.8
AYY59919.1\|2344300_2344504_+	hypothetical protein	E5E3N9	Burkholderia_phage	1.6e-27	98.5
AYY59920.1\|2344547_2344742_+	hypothetical protein	K4NXL1	Burkholderia_phage	3.7e-18	79.4
AYY59921.1\|2344746_2345106_+	hypothetical protein	E5E3N7	Burkholderia_phage	3.7e-40	73.1
AYY59922.1\|2345102_2345366_+	hypothetical protein	E5E3N6	Burkholderia_phage	1.3e-34	91.7
AYY59923.1\|2345368_2348164_+	hypothetical protein	E5E3N5	Burkholderia_phage	0.0e+00	96.6
AYY60910.1\|2348783_2349053_+	DUF4224 domain-containing protein	NA	NA	NA	NA
AYY59924.1\|2349053_2350133_+\|integrase	integrase	A0A2H4J8E5	uncultured_Caudovirales_phage	1.2e-28	30.7
AYY59925.1\|2350409_2351378_-	LysR family transcriptional regulator	NA	NA	NA	NA
AYY59926.1\|2351528_2352497_-	GlxA family transcriptional regulator	NA	NA	NA	NA
AYY59927.1\|2352610_2353294_+	DJ-1/PfpI family protein	NA	NA	NA	NA
AYY59928.1\|2353373_2353685_+	cupin domain-containing protein	NA	NA	NA	NA
2353625:2353643	attR	NA	NA	NA	NA
AYY59929.1\|2354126_2357234_+	glycosyl transferase family 51	NA	NA	NA	NA
AYY59930.1\|2357262_2357610_-	GFA family protein	NA	NA	NA	NA
AYY59931.1\|2357918_2359304_+	MFS transporter	NA	NA	NA	NA
AYY60911.1\|2359412_2359658_+	DUF1272 domain-containing protein	NA	NA	NA	NA
AYY59932.1\|2359770_2360481_-	FadR family transcriptional regulator	NA	NA	NA	NA
AYY59933.1\|2360599_2361748_+	galactonate dehydratase	Q6A202	Oenococcus_phage	1.6e-55	33.8
AYY59934.1\|2361864_2362797_-	copper resistance protein CopD	NA	NA	NA	NA
AYY59935.1\|2363184_2363949_+	cytochrome c4	NA	NA	NA	NA
AYY59936.1\|2363968_2365267_+	cytochrome c	NA	NA	NA	NA
AYY59937.1\|2365398_2366841_+	DEAD/DEAH box helicase	Q5GF26	Diachasmimorpha_longicaudata_entomopoxvirus	1.1e-53	34.4
AYY59938.1\|2366834_2367341_-	hypothetical protein	NA	NA	NA	NA
AYY59939.1\|2367408_2368236_-	ferritin-like domain-containing protein	NA	NA	NA	NA
AYY59940.1\|2368526_2370167_-	gamma-glutamyltransferase family protein	NA	NA	NA	NA
AYY59941.1\|2370369_2371149_+	DeoR/GlpR transcriptional regulator	A0A077SK06	Escherichia_phage	2.4e-23	29.8
AYY59942.1\|2371438_2371648_+\|tail	phage tail assembly chaperone	NA	NA	NA	NA

DBSCAN-SWA_4

3272337 : 3334110

uncultured_Caudovirales_phage(50.0%)

plate,tail,protease

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AYY60723.1\|3272337_3272859_+\|protease	ClpXP protease specificity-enhancing factor	A0A1S5R3H3	Pseudomonas_phage	7.4e-21	53.0
AYY60724.1\|3273095_3274100_+	hypothetical protein	NA	NA	NA	NA
AYY60725.1\|3274339_3275212_+	hypothetical protein	NA	NA	NA	NA
AYY60726.1\|3275639_3275906_+	hypothetical protein	NA	NA	NA	NA
AYY60727.1\|3277930_3278134_+	hypothetical protein	NA	NA	NA	NA
AYY60728.1\|3278246_3279047_-	cystine ABC transporter substrate-binding protein	NA	NA	NA	NA
AYY60987.1\|3279075_3279270_+	hypothetical protein	NA	NA	NA	NA
AYY60729.1\|3279378_3282558_+	type VI secretion system tip protein VgrG	A0A2H4JCD4	uncultured_Caudovirales_phage	7.9e-57	26.7
AYY60730.1\|3288143_3288569_+	SMI1/KNR4 family protein	NA	NA	NA	NA
AYY60731.1\|3288682_3288991_+	hypothetical protein	NA	NA	NA	NA
AYY60732.1\|3290015_3290366_+	hypothetical protein	NA	NA	NA	NA
AYY60733.1\|3291881_3292136_+	hypothetical protein	NA	NA	NA	NA
AYY60734.1\|3294661_3294784_+	DUF4237 domain-containing protein	NA	NA	NA	NA
AYY60735.1\|3297128_3297911_-	DotU family type IV/VI secretion system protein	NA	NA	NA	NA
AYY60736.1\|3297907_3299254_-\|plate	type VI secretion system baseplate subunit TssK	NA	NA	NA	NA
AYY60737.1\|3299356_3299968_-	type VI secretion system lipoprotein TssJ	NA	NA	NA	NA
AYY60738.1\|3300343_3300976_+	hypothetical protein	NA	NA	NA	NA
AYY60739.1\|3301011_3301527_+	type VI secretion system contractile sheath small subunit	NA	NA	NA	NA
AYY60740.1\|3301542_3303033_+	type VI secretion system contractile sheath large subunit	NA	NA	NA	NA
AYY60741.1\|3303104_3303608_+	type VI secretion system tube protein Hcp	NA	NA	NA	NA
AYY60742.1\|3303670_3304156_+\|plate	type VI secretion system baseplate subunit TssE	NA	NA	NA	NA
AYY60743.1\|3304234_3306070_+\|plate	type VI secretion system baseplate subunit TssF	NA	NA	NA	NA
AYY60744.1\|3306033_3307134_+\|plate	type VI secretion system baseplate subunit TssG	NA	NA	NA	NA
AYY60745.1\|3307178_3309848_+	type VI secretion system ATPase TssH	A0A223W0B1	Agrobacterium_phage	1.0e-89	30.8
AYY60746.1\|3309886_3311008_+	type VI secretion system protein TssA	NA	NA	NA	NA
AYY60747.1\|3311075_3313637_+	type VI secretion system tip protein VgrG	A0A2H4JCD4	uncultured_Caudovirales_phage	6.8e-51	28.6
AYY60748.1\|3313636_3314677_+	DUF4123 domain-containing protein	NA	NA	NA	NA
AYY60749.1\|3314867_3315704_+	DUF3304 domain-containing protein	NA	NA	NA	NA
AYY60750.1\|3315696_3317979_+	hypothetical protein	NA	NA	NA	NA
AYY60988.1\|3318032_3319388_+	DUF3304 domain-containing protein	NA	NA	NA	NA
AYY60989.1\|3320914_3321682_+	hypothetical protein	NA	NA	NA	NA
AYY60751.1\|3322135_3322951_+	DUF3304 domain-containing protein	NA	NA	NA	NA
AYY60752.1\|3323165_3323999_+	DUF3304 domain-containing protein	NA	NA	NA	NA
AYY60753.1\|3324143_3324917_+	hypothetical protein	NA	NA	NA	NA
AYY60754.1\|3325969_3326920_-	OmpA family protein	NA	NA	NA	NA
AYY60755.1\|3326924_3327914_-	type VI secretion system-associated protein TagF	NA	NA	NA	NA
AYY60756.1\|3327910_3331858_-	type VI secretion system membrane subunit TssM	NA	NA	NA	NA
AYY60757.1\|3332156_3333002_-	M15 family peptidase	NA	NA	NA	NA
AYY60758.1\|3333123_3334110_+\|tail	phage tail protein	NA	NA	NA	NA

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage