CRISPRimmunity

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Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Self targeting spacer number	Target MGE spacer number	Prophage number	Anti-CRISPR protein number
CP034433	Iodobacter sp. H11R3 chromosome, complete genome	0 crisprs	cas3,csa3,DinG,DEDDh	0	0	4	0

Results visualization

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CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

610589 : 708999

Burkholderia_phage(21.95%)

capsid,integrase,transposase,portal,terminase,tail,lysis,plate,protease

attL 622786:622810|attR 691039:691063

The bacterium proteins that are colored denote the protein is present at specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin' and 'tRNA')

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AZN35481.1\|610589_611339_+\|transposase	IS5 family transposase	NA	NA	NA	NA
AZN35482.1\|611353_612441_-\|transposase	IS3 family transposase	S5WIU1	Leptospira_phage	1.3e-43	37.5
AZN35483.1\|612796_613141_-	hypothetical protein	NA	NA	NA	NA
AZN35484.1\|613639_614545_-	hypothetical protein	NA	NA	NA	NA
AZN35485.1\|614685_618246_-	DEAD/DEAH box helicase	Q6NDX2	Leptospira_phage	6.6e-12	24.1
AZN35486.1\|618258_619458_-	restriction endonuclease subunit S	E3T4D5	Cafeteria_roenbergensis_virus	6.0e-10	26.1
AZN35487.1\|619454_621017_-	SAM-dependent DNA methyltransferase	A0A2H4UVB0	Bodo_saltans_virus	1.8e-22	29.0
AZN35488.1\|621121_622672_-	sigma-54-dependent Fis family transcriptional regulator	NA	NA	NA	NA
622786:622810	attL	NA	NA	NA	NA
AZN35489.1\|622853_623984_-	inovirus Gp2 family protein	NA	NA	NA	NA
AZN35490.1\|624242_625727_-	DUF3987 domain-containing protein	NA	NA	NA	NA
AZN35491.1\|625858_626074_-	AlpA family phage regulatory protein	A0A1V0E8E5	Vibrio_phage	2.3e-05	40.0
AZN35492.1\|626066_626708_-	hypothetical protein	NA	NA	NA	NA
AZN35493.1\|627165_628119_+	DUF932 domain-containing protein	A0A0H4INH5	Stenotrophomonas_phage	4.3e-51	39.7
AZN35494.1\|628196_629183_+	endonuclease	A6XMH8	Bacillus_virus	1.2e-51	39.2
AZN35495.1\|629179_630121_+	hypothetical protein	NA	NA	NA	NA
AZN35496.1\|630159_630828_+	hypothetical protein	NA	NA	NA	NA
AZN38049.1\|631267_634372_+	type VI secretion system tip protein VgrG	A0A2H4JCD4	uncultured_Caudovirales_phage	1.3e-88	33.1
AZN35497.1\|634451_638963_+	sugar-binding protein	A0A2H4JFP5	uncultured_Caudovirales_phage	1.8e-38	47.9
AZN35498.1\|638953_639514_+	hypothetical protein	NA	NA	NA	NA
AZN35499.1\|639675_640194_+	hypothetical protein	NA	NA	NA	NA
AZN35500.1\|640320_640677_+	hypothetical protein	NA	NA	NA	NA
AZN35501.1\|640683_641331_+	hypothetical protein	NA	NA	NA	NA
AZN35502.1\|641830_643033_+	hypothetical protein	A0A2H4JFP5	uncultured_Caudovirales_phage	2.1e-39	50.0
AZN35503.1\|643029_643401_+	hypothetical protein	NA	NA	NA	NA
AZN35504.1\|644375_645134_-	SDR family oxidoreductase	NA	NA	NA	NA
AZN35505.1\|645275_646190_+	LysR family transcriptional regulator	NA	NA	NA	NA
AZN35506.1\|646422_647406_+\|transposase	IS110 family transposase	Q75QL1	Wolbachia_phage	1.6e-16	28.4
AZN35507.1\|647478_648903_-\|integrase	site-specific integrase	A0A2H4IYX8	uncultured_Caudovirales_phage	1.1e-79	42.9
AZN35508.1\|649131_650298_-\|integrase	site-specific integrase	A0A059VF45	Pseudomonas_phage	3.3e-114	54.4
AZN35509.1\|650579_650819_-	hypothetical protein	NA	NA	NA	NA
AZN35510.1\|650906_652817_-	hypothetical protein	F1BUM9	Cronobacter_phage	3.6e-41	35.3
AZN35511.1\|652813_653272_-	hypothetical protein	NA	NA	NA	NA
AZN35512.1\|653355_653658_-	hypothetical protein	NA	NA	NA	NA
AZN35513.1\|653717_653954_-	hypothetical protein	NA	NA	NA	NA
AZN35514.1\|654040_654514_+	XRE family transcriptional regulator	NA	NA	NA	NA
AZN35515.1\|654613_654868_+	hypothetical protein	NA	NA	NA	NA
AZN35516.1\|654922_655192_-	type II toxin-antitoxin system RelE/ParE family toxin	NA	NA	NA	NA
AZN35517.1\|655175_655400_-	TraY domain-containing protein	NA	NA	NA	NA
AZN35518.1\|655637_655973_-	XRE family transcriptional regulator	A0A2D1GR59	Pseudomonas_phage	6.2e-05	35.5
AZN35519.1\|655969_656314_-	type II toxin-antitoxin system RelE/ParE family toxin	NA	NA	NA	NA
AZN35520.1\|656413_657469_-	phage late control D family protein	A0A1S5NV58	Burkholderia_phage	3.0e-106	58.9
AZN35521.1\|657465_657876_-	hypothetical protein	A0A1J0I2L5	Salmonella_phage	1.4e-30	50.4
AZN35522.1\|657875_660833_-\|tail	phage tail tape measure protein	A0A2H4JE61	uncultured_Caudovirales_phage	4.6e-160	42.2
AZN35523.1\|660829_661552_-	site-specific DNA-methyltransferase	V9IQV5	Stenotrophomonas_phage	9.4e-83	61.5
AZN35524.1\|661555_661672_-\|tail	GpE family phage tail protein	NA	NA	NA	NA
AZN35525.1\|661680_661986_-\|tail	phage tail assembly protein	E5E3Q1	Burkholderia_phage	1.7e-22	62.0
AZN35526.1\|662005_662524_-\|tail	phage major tail tube protein	E5E3U9	Burkholderia_phage	8.6e-54	60.5
AZN38050.1\|662539_663718_-\|tail	phage tail sheath protein	A0A077K9Y4	Ralstonia_phage	6.5e-142	63.5
AZN35527.1\|663808_664333_-	hypothetical protein	NA	NA	NA	NA
AZN35528.1\|664337_666791_-	hypothetical protein	F1BUP1	Erwinia_phage	2.2e-35	45.8
AZN35529.1\|666787_667333_-\|tail	phage tail protein I	A0A0F7LDF3	Escherichia_phage	1.5e-48	51.2
AZN35530.1\|667325_668225_-\|plate	baseplate assembly protein	E5G6N8	Salmonella_phage	1.1e-93	61.5
AZN35531.1\|668228_668579_-\|plate	baseplate assembly protein	V9IQW0	Stenotrophomonas_phage	2.4e-28	53.0
AZN38051.1\|668578_669262_-\|plate	phage baseplate assembly protein V	F1BUP5	Erwinia_phage	6.7e-30	52.1
AZN35532.1\|669374_670325_-	hypothetical protein	NA	NA	NA	NA
AZN35533.1\|670397_670850_-	phage virion morphogenesis protein	A0A1S5NPT5	Burkholderia_phage	5.0e-26	47.0
AZN35534.1\|670846_671308_-\|tail	phage tail protein	A0A077K8R3	Ralstonia_phage	6.9e-39	54.6
AZN35535.1\|671389_671848_-\|lysis	LysB family phage lysis regulatory protein	E5G6N2	Salmonella_phage	3.2e-12	36.7
AZN38052.1\|671844_672294_-	peptidase M15	C7BGD8	Burkholderia_phage	2.2e-26	45.9
AZN35536.1\|672124_672778_-	hypothetical protein	NA	NA	NA	NA
AZN35537.1\|672774_673143_-	hypothetical protein	A0A1S5NRL1	Burkholderia_phage	4.9e-19	45.8
AZN35538.1\|673147_673354_-\|tail	phage tail protein	A0A077K8R0	Ralstonia_phage	2.1e-16	61.8
AZN35539.1\|673923_674574_-\|terminase	terminase	A0A077K804	Ralstonia_phage	3.2e-58	52.4
AZN35540.1\|674573_675659_-\|capsid	phage major capsid protein, P2 family	K4NZP8	Burkholderia_phage	4.5e-105	56.7
AZN35541.1\|675729_676518_-\|capsid	GPO family capsid scaffolding protein	A0A077K9W8	Ralstonia_phage	2.0e-54	46.1
AZN35542.1\|676673_678425_+\|terminase	terminase ATPase subunit family protein	E5FFI8	Burkholderia_phage	1.5e-230	67.1
AZN35543.1\|678425_679448_+\|portal	phage portal protein	E5E3S7	Burkholderia_phage	4.4e-126	62.4
AZN35544.1\|679990_680257_+	hypothetical protein	NA	NA	NA	NA
AZN35545.1\|680265_680586_+	hypothetical protein	NA	NA	NA	NA
AZN35546.1\|680703_681462_+	hypothetical protein	NA	NA	NA	NA
AZN35547.1\|681468_682341_+	hypothetical protein	NA	NA	NA	NA
AZN35548.1\|682899_683910_-\|transposase	IS110 family transposase	A0A1S7J231	Thermus_phage	2.6e-22	33.1
AZN35549.1\|684317_684995_-	hypothetical protein	NA	NA	NA	NA
AZN35550.1\|684991_685603_-	hypothetical protein	NA	NA	NA	NA
AZN35551.1\|685599_686118_-	PAAR domain-containing protein	NA	NA	NA	NA
AZN35552.1\|687064_687565_+	hypothetical protein	NA	NA	NA	NA
AZN38053.1\|687729_687915_-\|transposase	transposase	NA	NA	NA	NA
AZN35553.1\|688043_688430_-	hypothetical protein	A0A218MNI5	uncultured_virus	1.0e-11	62.5
AZN35554.1\|691190_692273_-	hypothetical protein	NA	NA	NA	NA
691039:691063	attR	NA	NA	NA	NA
AZN35555.1\|692544_692994_+	PTS sugar transporter subunit IIA	NA	NA	NA	NA
AZN35556.1\|693002_693272_+	PTS sugar transporter subunit IIB	NA	NA	NA	NA
AZN35557.1\|693283_694540_+	PTS ascorbate transporter subunit IIC	NA	NA	NA	NA
AZN35558.1\|695027_695777_-\|transposase	IS5 family transposase	NA	NA	NA	NA
AZN35559.1\|698524_699343_+	DUF4123 domain-containing protein	NA	NA	NA	NA
AZN35560.1\|699427_699676_+	hypothetical protein	NA	NA	NA	NA
AZN35561.1\|699609_699876_+	DUF3304 domain-containing protein	NA	NA	NA	NA
AZN35562.1\|700003_700444_+	DUF3304 domain-containing protein	NA	NA	NA	NA
AZN35563.1\|700511_702416_+	hypothetical protein	NA	NA	NA	NA
AZN35564.1\|702796_703000_+	hypothetical protein	NA	NA	NA	NA
AZN35565.1\|703002_704166_+	hypothetical protein	NA	NA	NA	NA
AZN35566.1\|704567_706472_+\|protease	rhomboid family intramembrane serine protease	NA	NA	NA	NA
AZN35567.1\|706793_707881_-\|transposase	IS3 family transposase	S5WIU1	Leptospira_phage	1.4e-45	38.7
AZN35568.1\|708249_708999_-\|transposase	IS5 family transposase	NA	NA	NA	NA

DBSCAN-SWA_2

1237623 : 1248179

Tetraselmis_virus(33.33%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AZN35976.1\|1237623_1238316_-	lipoprotein-releasing ABC transporter ATP-binding protein LolD	G9BWD6	Planktothrix_phage	3.7e-36	40.2
AZN35977.1\|1238308_1239547_-	lipoprotein-releasing ABC transporter permease subunit	NA	NA	NA	NA
AZN35978.1\|1239705_1240491_-	pyruvate formate lyase-activating protein	A0A2P0VNQ0	Tetraselmis_virus	1.1e-25	31.9
AZN35979.1\|1240745_1240931_-	autonomous glycyl radical cofactor GrcA	A0A060AHY5	Cronobacter_phage	1.2e-15	75.0
AZN35980.1\|1241026_1243339_-	formate C-acetyltransferase	A0A2P0VNR5	Tetraselmis_virus	1.3e-170	42.4
AZN35981.1\|1243602_1245192_-	peptide chain release factor 3	A0A1B0RXH7	Streptococcus_phage	8.8e-33	28.2
AZN35982.1\|1245287_1248179_-	PAS domain S-box protein	A0A1V0SGX0	Hokovirus	2.6e-22	24.6

DBSCAN-SWA_3

2492322 : 2501411

Bacillus_virus(33.33%)

tRNA

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AZN36918.1\|2492322_2494932_-	EAL domain-containing protein	G3MA91	Bacillus_virus	2.2e-20	32.3
AZN36919.1\|2495051_2497358_-	type I DNA topoisomerase	A0A0G2YA05	Acanthamoeba_polyphaga_mimivirus	3.0e-98	36.3
AZN36920.1\|2497609_2498686_-	DNA-protecting protein DprA	S6BFL3	Thermus_phage	6.6e-24	33.8
AZN36921.1\|2498685_2499168_-	hypothetical protein	NA	NA	NA	NA
AZN36922.1\|2499127_2499745_-	LysM peptidoglycan-binding domain-containing protein	G3MBQ1	Bacillus_virus	7.4e-12	34.5
AZN36923.1\|2499893_2500397_+	peptide deformylase	E3SLL2	Synechococcus_phage	1.1e-16	39.7
AZN36924.1\|2500484_2501411_+\|tRNA	methionyl-tRNA formyltransferase	E3SNR5	Prochlorococcus_phage	3.5e-05	34.9

DBSCAN-SWA_4

2586605 : 2593723

Lake_Baikal_phage(28.57%)

protease

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AZN36990.1\|2586605_2587829_+	NADP-dependent isocitrate dehydrogenase	Q77Z09	Phage_21	1.1e-09	61.1
AZN36991.1\|2587959_2588163_-	cold-shock protein	A0A2H4N7Y6	Lake_Baikal_phage	1.8e-20	71.2
AZN36992.1\|2588277_2588481_-	cold-shock protein	A0A2H4N7Y6	Lake_Baikal_phage	1.1e-20	72.7
AZN36993.1\|2588790_2589102_+\|protease	ATP-dependent Clp protease adapter ClpS	A0A1B1IT64	uncultured_Mediterranean_phage	1.6e-10	40.0
AZN36994.1\|2589098_2591363_+\|protease	ATP-dependent Clp protease ATP-binding subunit ClpA	A0A223W0B1	Agrobacterium_phage	9.2e-169	42.1
AZN36995.1\|2591429_2592254_-	mechanosensitive ion channel family protein	NA	NA	NA	NA
AZN36996.1\|2592317_2593241_-	site-specific tyrosine recombinase XerD	A0A0K2CP59	Brevibacillus_phage	1.3e-28	27.3
AZN36997.1\|2593237_2593723_-	methylated-DNA--[protein]-cysteine S-methyltransferase	A0A2P1EL10	Moumouvirus	2.0e-12	31.9

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage