CRISPRimmunity

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Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Self targeting spacer number	Target MGE spacer number	Prophage number	Anti-CRISPR protein number
CP034842	Fusobacterium necrophorum subsp. necrophorum strain ATCC 25286 chromosome, complete genome	0 crisprs	WYL,cas14j,cas6,cas8b2,cas7,cas5,cas3,cas4,cas1,cas2,DEDDh,csm3gr7,csa3,PD-DExK,csm5gr7,csm4gr5,csm2gr11,cas10,csx20,csx1,DinG	0	0	5	0

Results visualization

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CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

490723 : 531260

Clostridium_phage(34.78%)

plate,portal,integrase,terminase,head,tail

attL 489278:489337|attR 516105:517549

The bacterium proteins that are colored denote the protein is present at specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin' and 'tRNA')

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
489278:489337	attL	NA	NA	NA	NA
AZW08558.1\|490723_491848_-\|integrase	site-specific integrase	Q77YW7	Streptococcus_phage	7.1e-21	25.1
AZW08559.1\|491987_492695_-	hypothetical protein	NA	NA	NA	NA
AZW08560.1\|492711_493134_-	XRE family transcriptional regulator	NA	NA	NA	NA
AZW08561.1\|493276_493468_+	XRE family transcriptional regulator	A0A1W6JPU6	Staphylococcus_phage	3.8e-07	44.4
AZW08562.1\|493637_493904_+	hypothetical protein	NA	NA	NA	NA
AZW08563.1\|493870_494185_-	hypothetical protein	NA	NA	NA	NA
AZW08564.1\|494273_494609_+	hypothetical protein	NA	NA	NA	NA
AZW08565.1\|494624_495371_+	oxidoreductase	A0A2H4J9M3	uncultured_Caudovirales_phage	1.5e-67	55.3
AZW08566.1\|495543_495741_+	DNA-binding protein	NA	NA	NA	NA
AZW08567.1\|495847_496114_+	hypothetical protein	NA	NA	NA	NA
AZW08568.1\|496264_497038_+	hypothetical protein	NA	NA	NA	NA
AZW08569.1\|497039_497495_+	hypothetical protein	NA	NA	NA	NA
AZW08570.1\|497494_498664_+	DUF2800 domain-containing protein	H7BVP9	unidentified_phage	3.5e-87	45.0
AZW08571.1\|498686_499265_+	DUF2815 family protein	S5MC21	Brevibacillus_phage	9.6e-38	47.5
AZW08572.1\|499310_501281_+	hypothetical protein	H7BVQ1	unidentified_phage	2.8e-206	54.9
AZW08573.1\|501290_501563_+	hypothetical protein	A0A0A7RTP9	Clostridium_phage	1.6e-11	39.1
AZW08574.1\|501752_504218_+	virulence-associated protein E	A0A0A7RTG3	Clostridium_phage	4.1e-186	42.1
AZW08575.1\|504505_504802_+	VRR-NUC domain-containing protein	S5MUC8	Brevibacillus_phage	1.1e-16	50.0
AZW08576.1\|504815_506195_+	DEAD/DEAH box helicase	S5MA26	Brevibacillus_phage	6.3e-144	54.9
AZW08577.1\|506169_506481_+	hypothetical protein	NA	NA	NA	NA
AZW10282.1\|506703_507129_+	hypothetical protein	NA	NA	NA	NA
AZW08578.1\|507139_507649_+	hypothetical protein	NA	NA	NA	NA
AZW08579.1\|507808_508633_+	LuxR family transcriptional regulator	NA	NA	NA	NA
AZW08580.1\|508840_509350_+\|terminase	terminase small subunit	A0A0A0RSW5	Bacillus_phage	3.9e-27	39.9
AZW08581.1\|510777_512082_+\|portal	phage portal protein	A0A0M4S669	Bacillus_phage	9.3e-81	42.3
AZW08582.1\|512059_513493_+\|head	phage head morphogenesis protein	A0A2H4J4W7	uncultured_Caudovirales_phage	6.3e-30	30.8
AZW08583.1\|513485_513680_+	hypothetical protein	NA	NA	NA	NA
AZW08584.1\|513758_513956_+	hypothetical protein	NA	NA	NA	NA
AZW08585.1\|513971_514361_+	hypothetical protein	NA	NA	NA	NA
AZW08586.1\|514460_514652_+	type II toxin-antitoxin system HicA family toxin	NA	NA	NA	NA
AZW08587.1\|514685_515114_+	type II toxin-antitoxin system HicB family antitoxin	A0A0C5AN56	Paenibacillus_phage	3.4e-16	37.5
AZW08588.1\|515235_516084_+	ABC transporter ATP-binding protein	NA	NA	NA	NA
AZW08589.1\|517621_517798_+	dihydrolipoamide acetyltransferase	NA	NA	NA	NA
516105:517549	attR	NA	NA	NA	NA
AZW08590.1\|517905_518805_+	phage regulatory protein	A0A141VTQ8	Staphylococcus_phage	4.8e-20	51.0
AZW08591.1\|518863_519562_+	transcriptional regulator	F8UBN0	Clostridium_phage	2.3e-17	33.5
AZW08592.1\|519848_520412_+	DUF4355 domain-containing protein	NA	NA	NA	NA
AZW08593.1\|520413_521553_+	hypothetical protein	NA	NA	NA	NA
AZW08594.1\|521567_521780_+	hypothetical protein	NA	NA	NA	NA
AZW08595.1\|521819_522116_+	hypothetical protein	C1KFI4	Lactobacillus_virus	5.5e-13	43.7
AZW08596.1\|522140_522464_+	hypothetical protein	NA	NA	NA	NA
AZW08597.1\|522460_522817_+	hypothetical protein	NA	NA	NA	NA
AZW08598.1\|522813_523191_+	HK97 gp10 family phage protein	A0A0A7RTT0	Clostridium_phage	2.8e-06	37.4
AZW08599.1\|523187_523655_+	hypothetical protein	NA	NA	NA	NA
AZW08600.1\|523665_524745_+\|tail	phage tail sheath protein	A0A0A8WJL8	Clostridium_phage	8.9e-37	32.3
AZW08601.1\|524759_525200_+\|terminase	terminase	A0A0A8WF55	Clostridium_phage	3.5e-32	50.4
AZW08602.1\|525211_525586_+	regulator	NA	NA	NA	NA
AZW08603.1\|525931_527806_+\|tail	phage tail tape measure protein	H9A124	Staphylococcus_phage	1.1e-39	31.2
AZW08604.1\|527817_528258_+	peptidoglycan-binding protein	NA	NA	NA	NA
AZW08605.1\|528263_529313_+\|terminase	terminase	NA	NA	NA	NA
AZW08606.1\|529309_529759_+	DUF2577 domain-containing protein	NA	NA	NA	NA
AZW08607.1\|529759_530197_+	DUF2634 domain-containing protein	A0A0A7RUS1	Clostridium_phage	3.7e-18	32.9
AZW08608.1\|530186_531260_+\|plate	baseplate J/gp47 family protein	A0A0A7RUN3	Clostridium_phage	4.5e-41	33.8

DBSCAN-SWA_2

878207 : 883985

Bacillus_phage(28.57%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AZW08890.1\|878207_879176_+	KpsF/GutQ family sugar-phosphate isomerase	A0A2P0VNK5	Tetraselmis_virus	2.3e-31	31.8
AZW08891.1\|879442_880993_+	DUF2828 family protein	A0A218KCC5	Bacillus_phage	6.3e-68	32.5
AZW08892.1\|881123_881606_-	NADPH-dependent 7-cyano-7-deazaguanine reductase QueF	E7DN65	Pneumococcus_phage	1.8e-50	72.1
AZW08893.1\|881606_882302_-	7-cyano-7-deazaguanine synthase QueC	A0A1U9WRB5	Streptococcus_virus	1.0e-65	56.0
AZW08894.1\|882310_882886_-	GTP cyclohydrolase I FolE	S4U0J3	uncultured_phage	3.6e-45	52.3
AZW08895.1\|882886_883555_-	putative 7-carboxy-7-deazaguanine synthase QueE	S4TZT1	uncultured_phage	2.5e-45	47.9
AZW08896.1\|883541_883985_-	6-carboxytetrahydropterin synthase QueD	J9PV91	Bacillus_phage	5.1e-07	30.2

DBSCAN-SWA_3

936889 : 989000

unidentified_phage(20.0%)

transposase

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AZW08935.1\|936889_937658_+\|transposase	IS5 family transposase	A0A0N9SHJ4	Paenibacillus_phage	1.8e-23	40.0
AZW08936.1\|937706_937862_+	hypothetical protein	NA	NA	NA	NA
AZW08937.1\|937759_938146_+	dTDP-4-keto-6-deoxy-D-glucose epimerase	NA	NA	NA	NA
AZW08938.1\|938270_938525_+	hypothetical protein	NA	NA	NA	NA
AZW08939.1\|940667_941078_+	hypothetical protein	NA	NA	NA	NA
AZW08940.1\|941120_941315_+	hypothetical protein	NA	NA	NA	NA
AZW08941.1\|941348_941588_+	hypothetical protein	NA	NA	NA	NA
AZW08942.1\|942270_943830_+	O-antigen ligase domain-containing protein	NA	NA	NA	NA
AZW08943.1\|944525_945206_+	sugar transferase	NA	NA	NA	NA
AZW08944.1\|945205_946099_+	NAD-dependent epimerase/dehydratase family protein	NA	NA	NA	NA
AZW08945.1\|946095_947232_+	glycosyltransferase family 1 protein	NA	NA	NA	NA
AZW08946.1\|947228_948038_+	glycosyltransferase	NA	NA	NA	NA
AZW08947.1\|949273_950215_+	NAD(P)-dependent oxidoreductase	NA	NA	NA	NA
AZW08948.1\|950219_951014_+	sugar phosphate isomerase/epimerase	NA	NA	NA	NA
AZW08949.1\|951038_952163_+	FAD-binding oxidoreductase	NA	NA	NA	NA
AZW08950.1\|952186_953095_+	glycosyl transferase family 2	NA	NA	NA	NA
AZW08951.1\|953757_954699_+	capsular biosynthesis protein	A0A2H4UTT0	Bodo_saltans_virus	1.4e-06	31.6
AZW10294.1\|954893_955718_+	hypothetical protein	NA	NA	NA	NA
AZW08952.1\|955741_957004_+	polysaccharide biosynthesis protein	NA	NA	NA	NA
AZW08953.1\|957016_957961_+	hypothetical protein	NA	NA	NA	NA
AZW08954.1\|957984_958698_+	acylneuraminate cytidylyltransferase family protein	A0A1B1IVD3	uncultured_Mediterranean_phage	6.3e-15	30.1
AZW08955.1\|958736_959501_+\|transposase	ISL3 family transposase	A0A1S5SBP9	Streptococcus_phage	2.8e-29	36.7
AZW08956.1\|959451_959769_+	hypothetical protein	NA	NA	NA	NA
AZW08957.1\|960706_961462_-	hypothetical protein	NA	NA	NA	NA
AZW08958.1\|961467_962568_-	UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing)	A0A1V0SAG5	Catovirus	4.8e-30	30.0
AZW08959.1\|962621_964118_-	hypothetical protein	NA	NA	NA	NA
AZW08960.1\|964114_965296_-	4Fe-4S dicluster domain-containing protein	NA	NA	NA	NA
AZW08961.1\|965298_966498_-	polysaccharide pyruvyl transferase family protein	NA	NA	NA	NA
AZW08962.1\|966564_967668_-	EpsG family protein	NA	NA	NA	NA
AZW08963.1\|967664_968750_-	glycosyl transferase family 1	NA	NA	NA	NA
AZW08964.1\|968820_969594_-	glycosyltransferase family 2 protein	NA	NA	NA	NA
AZW08965.1\|969606_970830_-	DegT/DnrJ/EryC1/StrS family aminotransferase	NA	NA	NA	NA
AZW08966.1\|970848_971436_-	sugar transferase	NA	NA	NA	NA
AZW08967.1\|971440_972541_-	polysaccharide biosynthesis protein	A0A2P1ELS8	Moumouvirus	5.7e-31	30.1
AZW08968.1\|972782_973760_+	hypothetical protein	NA	NA	NA	NA
AZW08969.1\|975342_976371_-\|transposase	IS30 family transposase	H7BUM7	unidentified_phage	4.1e-55	39.7
AZW08970.1\|976478_976661_+	CopG family transcriptional regulator	NA	NA	NA	NA
AZW08971.1\|978092_979403_-\|transposase	ISL3 family transposase	NA	NA	NA	NA
AZW08972.1\|979911_980097_-	DNA-binding protein	NA	NA	NA	NA
AZW08973.1\|980101_980464_-	DUF1353 domain-containing protein	X2KQ25	Campylobacter_phage	4.1e-10	38.0
AZW08974.1\|980615_980861_+	DNA-binding protein	NA	NA	NA	NA
AZW08975.1\|980971_981265_+	hypothetical protein	NA	NA	NA	NA
AZW08976.1\|981285_981960_-	DUF1275 domain-containing protein	NA	NA	NA	NA
AZW08977.1\|981956_982274_-	hypothetical protein	NA	NA	NA	NA
AZW08978.1\|982422_983133_+	hypothetical protein	NA	NA	NA	NA
AZW08979.1\|983329_983842_+	translation initiation factor IF-3	A0A2L0UZ54	Agrobacterium_phage	8.3e-09	28.8
AZW08980.1\|983885_984092_+	50S ribosomal protein L35	NA	NA	NA	NA
AZW08981.1\|984109_984460_+	50S ribosomal protein L20	NA	NA	NA	NA
AZW08982.1\|984529_985213_+	FadR family transcriptional regulator	NA	NA	NA	NA
AZW08983.1\|985340_986132_+	FadR family transcriptional regulator	NA	NA	NA	NA
AZW08984.1\|986329_986536_-	hypothetical protein	NA	NA	NA	NA
AZW08985.1\|986681_987596_-\|transposase	ISL3 family transposase	NA	NA	NA	NA
AZW08986.1\|987971_989000_+\|transposase	IS30 family transposase	H7BUM7	unidentified_phage	1.4e-55	39.7

DBSCAN-SWA_4

1577341 : 1585895

Streptococcus_phage(28.57%)

transposase

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AZW09422.1\|1577341_1578028_-	segregation/condensation protein A	A0A1B1IVW1	uncultured_Mediterranean_phage	9.7e-05	25.0
AZW09423.1\|1577996_1578962_-	bifunctional riboflavin kinase/FAD synthetase	A0A1V0SD03	Indivirus	2.1e-13	39.0
AZW09424.1\|1578973_1579864_-	DNA-binding protein WhiA	Q7AWZ3	Streptococcus_phage	3.1e-19	26.9
AZW09425.1\|1579876_1582561_-	DNA polymerase I	A0A218MKQ4	uncultured_virus	1.2e-50	29.2
AZW09426.1\|1582752_1583208_+	single-stranded DNA-binding protein	F8UBF4	Clostridium_phage	3.1e-23	49.5
AZW09427.1\|1583231_1583780_+	cob(I)yrinic acid a,c-diamide adenosyltransferase	NA	NA	NA	NA
AZW09428.1\|1584015_1584426_-\|transposase	IS200/IS605 family transposase	A0A286QN76	Streptococcus_phage	9.1e-51	71.4
AZW09429.1\|1584899_1585895_-	DUF4065 domain-containing protein	A0A0N9SGM1	Paenibacillus_phage	1.6e-16	36.4

DBSCAN-SWA_5

1755253 : 1762967

Prochlorococcus_phage(42.86%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
AZW09554.1\|1755253_1755874_+	MBL fold metallo-hydrolase	A0A1X9I5D3	Streptococcus_phage	9.7e-20	35.0
AZW09555.1\|1756043_1757270_-	phosphoribosylamine--glycine ligase	NA	NA	NA	NA
AZW09556.1\|1757287_1758790_-	bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase	Q58MG4	Prochlorococcus_phage	2.7e-68	45.9
AZW09557.1\|1758791_1759352_-	phosphoribosylglycinamide formyltransferase	R9S626	Prochlorococcus_phage	5.0e-23	35.7
AZW09558.1\|1759339_1760356_-	phosphoribosylformylglycinamidine cyclo-ligase	Q58MH8	Prochlorococcus_phage	1.4e-68	46.2
AZW09559.1\|1760369_1761719_-	amidophosphoribosyltransferase	A0A0M3SGR2	Mollivirus	3.8e-45	32.5
AZW09560.1\|1761746_1762463_-	phosphoribosylaminoimidazolesuccinocarboxamide synthase	A0A0E3HI61	Synechococcus_phage	5.2e-41	41.9
AZW09561.1\|1762490_1762967_-	5-(carboxyamino)imidazole ribonucleotide mutase	A0A2P0VNU7	Tetraselmis_virus	2.0e-28	47.4

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage