CRISPRimmunity

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Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Self targeting spacer number	Target MGE spacer number	Prophage number	Anti-CRISPR protein number
CP035110	Lactobacillus curvatus strain SRCM103465 chromosome, complete genome	0 crisprs	cas3,DEDDh,DinG,csa3	0	0	4	0

Results visualization

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CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

761663 : 803585

Lactobacillus_phage(23.68%)

integrase,tail,portal,protease,capsid,terminase

attL 763448:763502|attR 804017:804071

The bacterium proteins that are colored denote the protein is present at specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin' and 'tRNA')

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
QAR35229.1\|761663_763502_+	elongation factor 4	E4ZFJ7	Streptococcus_phage	1.2e-20	25.8
763448:763502	attL	NA	NA	NA	NA
QAR35230.1\|763632_764796_-\|integrase	site-specific integrase	A0A2I7SCV1	Paenibacillus_phage	2.2e-97	47.8
QAR35231.1\|764882_765173_-	hypothetical protein	NA	NA	NA	NA
QAR35232.1\|765245_766067_-	hypothetical protein	NA	NA	NA	NA
QAR35233.1\|766128_766590_-	toxin	A0A0A7RTX7	Clostridium_phage	1.2e-19	46.2
QAR35234.1\|766601_766937_-	XRE family transcriptional regulator	A8ATX5	Listeria_phage	4.6e-16	44.4
QAR35235.1\|767094_767313_+	XRE family transcriptional regulator	NA	NA	NA	NA
QAR35236.1\|767566_768124_+	XRE family transcriptional regulator	O03909	Lactobacillus_phage	8.1e-42	54.5
QAR35237.1\|768124_768382_+	hypothetical protein	NA	NA	NA	NA
QAR35238.1\|768720_768993_+	hypothetical protein	NA	NA	NA	NA
QAR35239.1\|768982_769954_+	DUF1351 domain-containing protein	U5U3Y3	Lactobacillus_phage	3.3e-22	27.2
QAR35240.1\|769953_770946_+	hypothetical protein	A0A0S2SY47	Bacillus_phage	4.0e-60	40.1
QAR35241.1\|770945_771635_+	hypothetical protein	E9LUU3	Lactobacillus_phage	1.4e-56	49.3
QAR35242.1\|771624_772098_+	single-stranded DNA-binding protein	A0A2D1GPB7	Lactobacillus_phage	6.0e-46	61.8
QAR35243.1\|772129_772855_+	DnaD domain protein	A0A1L2JY26	Aeribacillus_phage	3.2e-14	37.5
QAR35244.1\|773066_773597_+	response regulator transcription factor	NA	NA	NA	NA
QAR35245.1\|773565_773775_+	hypothetical protein	NA	NA	NA	NA
QAR35246.1\|773932_774244_+	hypothetical protein	NA	NA	NA	NA
QAR35247.1\|774440_774638_+	hypothetical protein	NA	NA	NA	NA
QAR35248.1\|774868_775108_+	hypothetical protein	NA	NA	NA	NA
QAR35249.1\|775605_775872_+	hypothetical protein	NA	NA	NA	NA
QAR35250.1\|776056_776305_+	hypothetical protein	NA	NA	NA	NA
QAR35251.1\|776301_776502_+	hypothetical protein	NA	NA	NA	NA
QAR36383.1\|776507_776816_+	hypothetical protein	A0A2K9VCF6	Lactobacillus_phage	3.0e-22	56.0
QAR35252.1\|777165_777627_+	autolysin	D2IZZ3	Enterococcus_phage	8.8e-18	39.1
QAR36384.1\|778648_778924_+	DUF2829 domain-containing protein	I6X2Q9	Vibriophage	2.4e-31	67.4
QAR35253.1\|779062_779293_+	hypothetical protein	NA	NA	NA	NA
QAR35254.1\|779330_780215_+\|terminase	terminase	V9QKX0	Oenococcus_phage	6.1e-60	46.2
QAR35255.1\|780198_781482_+\|terminase	PBSX family phage terminase large subunit	V5UQR5	Oenococcus_phage	7.8e-189	71.6
QAR35256.1\|781492_782941_+\|portal	phage portal protein	V9QKI8	Oenococcus_phage	2.3e-149	55.4
QAR35257.1\|782882_783197_+\|protease	ribosomal-processing cysteine protease Prp	D2J003	Enterococcus_phage	5.6e-08	39.0
QAR35258.1\|783199_784375_+	hypothetical protein	V5UTD3	Oenococcus_phage	6.8e-91	47.2
QAR35259.1\|784543_785191_+	DUF4355 domain-containing protein	V5URU4	Oenococcus_phage	2.9e-19	45.4
QAR35260.1\|785203_785566_+	hypothetical protein	V5UTH9	Oenococcus_phage	2.1e-30	57.0
QAR35261.1\|785589_786651_+\|capsid	major capsid protein	V9QKJ3	Oenococcus_phage	3.0e-122	63.3
QAR36385.1\|786698_786986_+	hypothetical protein	A0A059NT99	Lactococcus_phage	2.7e-17	48.4
QAR35262.1\|786982_787369_+	hypothetical protein	D7RWJ2	Brochothrix_phage	3.3e-26	55.9
QAR35263.1\|787319_787865_+	HK97 gp10 family phage protein	V5URV0	Oenococcus_phage	5.3e-54	61.0
QAR35264.1\|787866_788232_+	hypothetical protein	Q6SE74	Lactobacillus_prophage	5.5e-23	42.5
QAR35265.1\|788244_788718_+\|tail	phage tail protein	V9QKJ6	Oenococcus_phage	4.4e-41	59.9
QAR35266.1\|788987_789392_+	hypothetical protein	A0A182BQ86	Lactococcus_phage	5.3e-35	59.3
QAR35267.1\|789484_789790_+	hypothetical protein	D7RWJ9	Brochothrix_phage	7.3e-13	42.4
QAR35268.1\|789972_790347_-	DUF2513 domain-containing protein	A0A2I7SCV0	Paenibacillus_phage	4.3e-15	39.4
QAR35269.1\|790404_795222_+\|tail	phage tail protein	D2J014	Enterococcus_phage	6.3e-175	44.9
QAR35270.1\|795237_795597_+	hypothetical protein	A0A1S5SCJ5	Streptococcus_phage	3.0e-29	55.5
QAR35271.1\|795609_797592_+	hypothetical protein	D7RWK2	Brochothrix_phage	3.6e-116	52.2
QAR36386.1\|797618_799616_+	hypothetical protein	A0A1B0Y2S0	Lactobacillus_phage	2.4e-80	39.5
QAR35272.1\|799612_800062_+	DUF1617 family protein	A0A2P0ZLE0	Lactobacillus_phage	6.6e-18	37.4
QAR35273.1\|800061_800298_+	hypothetical protein	NA	NA	NA	NA
QAR35274.1\|800318_801797_+	N-acetylmuramoyl-L-alanine amidase	Q9MCC8	Lactobacillus_phage	5.6e-50	56.0
QAR35275.1\|801836_802340_-	hypothetical protein	NA	NA	NA	NA
QAR35276.1\|802326_802557_-	hypothetical protein	B8R666	Lactobacillus_phage	2.6e-10	45.8
QAR35277.1\|802965_803214_+	hypothetical protein	A0A0S2MYI8	Enterococcus_phage	3.2e-06	34.3
QAR35278.1\|803210_803585_+	hypothetical protein	E3W8I0	Leuconostoc_phage	1.4e-05	31.1
804017:804071	attR	NA	NA	NA	NA

DBSCAN-SWA_2

810711 : 818944

Bacillus_phage(50.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
QAR35287.1\|810711_811038_-	heavy metal-binding domain-containing protein	M4STD1	Rhodobacter_phage	2.4e-06	35.6
QAR35288.1\|811053_811419_-	DUF1304 domain-containing protein	NA	NA	NA	NA
QAR35289.1\|811528_812524_-	UDP-glucose 4-epimerase GalE	A0A2K9L1R4	Tupanvirus	1.4e-49	37.4
QAR35290.1\|812676_814971_+	single-stranded-DNA-specific exonuclease RecJ	A7KV88	Bacillus_phage	3.9e-74	31.4
QAR35291.1\|815005_815524_+	adenine phosphoribosyltransferase	A0A1V0SKE5	Klosneuvirus	8.9e-27	42.0
QAR35292.1\|815741_816356_-	transcriptional repressor LexA	A0A1B1P7Q3	Bacillus_phage	1.3e-16	57.4
QAR35293.1\|816491_816734_+	DUF896 domain-containing protein	NA	NA	NA	NA
QAR35294.1\|816814_817042_+	YneF family protein	NA	NA	NA	NA
QAR35295.1\|817198_818944_+	ATP-binding cassette domain-containing protein	W8CYL7	Bacillus_phage	1.5e-46	27.9

DBSCAN-SWA_3

916152 : 924556

Paramecium_bursaria_Chlorella_virus(14.29%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
QAR35381.1\|916152_916872_+	aquaporin family protein	M1HQB9	Paramecium_bursaria_Chlorella_virus	8.3e-23	30.5
QAR35382.1\|917259_918633_+	glycoside hydrolase family 1 protein	A0A0B5JD41	Pandoravirus	3.3e-44	29.3
QAR35383.1\|918676_919156_-	nucleoside 2-deoxyribosyltransferase	A0A2P0ZL88	Lactobacillus_phage	6.5e-16	37.5
QAR35384.1\|919607_921641_+	potassium transporter Kup	M1IAJ4	Acanthocystis_turfacea_Chlorella_virus	1.7e-68	35.6
QAR35385.1\|921866_922475_+	LysM peptidoglycan-binding domain-containing protein	A0A249XZV3	Enterococcus_phage	5.6e-20	48.2
QAR35386.1\|922616_922886_+	hypothetical protein	NA	NA	NA	NA
QAR36393.1\|922917_924183_+	Y-family DNA polymerase	M1Q231	Streptococcus_phage	6.9e-81	38.8
QAR35387.1\|924175_924556_+	hypothetical protein	E3W8I0	Leuconostoc_phage	8.3e-06	29.5

DBSCAN-SWA_4

1174441 : 1182323

Staphylococcus_phage(33.33%)

tRNA

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
QAR35630.1\|1174441_1175299_-	DegV family protein	A0A0N9SI50	Staphylococcus_phage	3.9e-19	41.9
QAR35631.1\|1175481_1175976_-	dihydrofolate reductase	G3MBI7	Bacillus_virus	2.5e-26	38.6
QAR35632.1\|1175991_1176942_-	thymidylate synthase	A0A0N9SH48	Staphylococcus_phage	2.8e-127	66.6
QAR35633.1\|1177567_1179451_-	ABC transporter ATP-binding protein	A0A2K9L3Z8	Tupanvirus	8.5e-51	30.6
QAR35634.1\|1179593_1180055_-	hypothetical protein	NA	NA	NA	NA
QAR35635.1\|1180118_1181318_-\|tRNA	CCA tRNA nucleotidyltransferase	H7BUW3	unidentified_phage	5.4e-43	50.0
QAR35636.1\|1181465_1182323_+	YitT family protein	M1Q1P6	Streptococcus_phage	3.9e-59	41.5

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage