CRISPRimmunity

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Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Target MGE spacer number	Prophage number
CP035137	Enterococcus faecium strain SRCM103341 plasmid unnamed1, complete sequence	0 crisprs	NA	0	1
CP035136	Enterococcus faecium strain SRCM103341 chromosome, complete genome	1 crisprs	cas1,cas2,csn2,DEDDh,cas3,csa3,DinG,cas14j	4	3
CP035138	Enterococcus faecium strain SRCM103341 plasmid unnamed2, complete sequence	0 crisprs	NA	0	0

Results visualization

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CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

15057 : 193670

162

Staphylococcus_phage(22.92%)

bacteriocin,protease,transposase,integrase

attL 29433:29455|attR 173018:173041

The bacterium proteins that are colored denote the protein is present at specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin' and 'tRNA')

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
QAR74426.1\|15057_16236_+\|transposase	IS256-like element ISEf1 family transposase	A0A220NQR7	Corynebacterium_phage	3.0e-30	25.6
QAR74427.1\|16380_16512_-\|bacteriocin	enterocin L50 family leaderless bacteriocin	NA	NA	NA	NA
QAR74428.1\|16531_16666_-\|bacteriocin	enterocin L50 family leaderless bacteriocin	NA	NA	NA	NA
QAR74429.1\|17173_17443_+	hypothetical protein	NA	NA	NA	NA
QAR74430.1\|17443_17716_+	hypothetical protein	NA	NA	NA	NA
QAR74431.1\|17705_18137_+	hypothetical protein	NA	NA	NA	NA
QAR74432.1\|18133_19519_+	hypothetical protein	NA	NA	NA	NA
QAR74433.1\|19520_20018_+	hypothetical protein	NA	NA	NA	NA
QAR74434.1\|20010_21228_+	efflux RND transporter periplasmic adaptor subunit	NA	NA	NA	NA
QAR74435.1\|21224_21908_+	ABC transporter ATP-binding protein	G9BWD6	Planktothrix_phage	8.7e-38	38.7
QAR74436.1\|21904_23104_+	FtsX-like permease family protein	NA	NA	NA	NA
QAR74437.1\|23507_23852_-	hypothetical protein	NA	NA	NA	NA
QAR74438.1\|23932_24436_-	peptidase	A0A1B2APZ3	Phage_Wrath	3.4e-39	55.0
QAR74439.1\|24398_24635_+	XRE family transcriptional regulator	A0A097PBE6	Streptococcus_pyogenes_phage	2.5e-13	52.1
QAR74440.1\|24871_26986_+\|protease	ATP-dependent Clp protease ATP-binding subunit	K4FB40	Cronobacter_phage	6.9e-118	38.7
QAR74441.1\|27131_27326_+	hypothetical protein	NA	NA	NA	NA
QAR74601.1\|27367_27598_-	hypothetical protein	NA	NA	NA	NA
29433:29455	attL	NA	NA	NA	NA
QAR74442.1\|29520_30711_+\|transposase	IS256 family transposase	A0A0N9STL0	Staphylococcus_phage	4.3e-24	28.4
29433:29455	attL	NA	NA	NA	NA
QAR74443.1\|31181_31736_+\|integrase	integrase	W8CYP1	Bacillus_phage	8.6e-36	46.1
QAR74444.1\|31908_32382_-\|integrase	integrase	A0A097PAS9	Streptococcus_pyogenes_phage	2.3e-13	44.2
QAR74445.1\|32393_33341_+	ring-cleaving dioxygenase	NA	NA	NA	NA
QAR74446.1\|35139_36165_+	LacI family transcriptional regulator	NA	NA	NA	NA
QAR74447.1\|36302_37568_+	extracellular solute-binding protein	NA	NA	NA	NA
QAR74448.1\|38562_39387_+	carbohydrate ABC transporter permease	NA	NA	NA	NA
QAR74449.1\|39418_40729_+	alpha-glucosidase/alpha-galactosidase	NA	NA	NA	NA
QAR74450.1\|42147_43020_+	sugar ABC transporter permease	NA	NA	NA	NA
QAR74451.1\|43031_43862_+	carbohydrate ABC transporter permease	NA	NA	NA	NA
QAR74452.1\|43901_46085_+	alpha-galactosidase	NA	NA	NA	NA
QAR74453.1\|46145_47366_+	oligo-1,6-glucosidase	NA	NA	NA	NA
QAR74454.1\|47362_48823_+	sucrose phosphorylase	NA	NA	NA	NA
QAR74455.1\|50327_50537_+	DUF3173 domain-containing protein	NA	NA	NA	NA
QAR74456.1\|50529_50679_+\|integrase	integrase	NA	NA	NA	NA
QAR74457.1\|51781_52867_-	tyrosine recombinase XerS	NA	NA	NA	NA
QAR74458.1\|53200_53434_+	addiction module antitoxin	NA	NA	NA	NA
QAR74459.1\|53430_53838_+	type II toxin-antitoxin system death-on-curing family toxin	A0A0N9SKD8	Staphylococcus_phage	4.0e-14	38.9
QAR74460.1\|54288_54798_-	hypothetical protein	NA	NA	NA	NA
QAR74461.1\|54938_55289_-	hypothetical protein	NA	NA	NA	NA
QAR74462.1\|55281_56607_-	Y-family DNA polymerase	M1Q231	Streptococcus_phage	1.8e-100	44.5
QAR74463.1\|56944_57235_+	hypothetical protein	NA	NA	NA	NA
QAR74602.1\|57490_57802_-	hypothetical protein	NA	NA	NA	NA
QAR74464.1\|59591_59789_+	hypothetical protein	NA	NA	NA	NA
QAR74603.1\|59881_60046_+\|bacteriocin	bacteriocin	NA	NA	NA	NA
QAR74465.1\|60050_60317_+\|bacteriocin	bacteriocin immunity protein	NA	NA	NA	NA
QAR74466.1\|60954_61643_-\|transposase	IS6-like element IS1216 family transposase	A0A1X9I6C6	Streptococcus_phage	1.5e-125	98.7
QAR74467.1\|62131_62476_-	type II toxin-antitoxin system PemK/MazF family toxin	NA	NA	NA	NA
QAR74468.1\|62469_62733_-	PbsX family transcriptional regulator	NA	NA	NA	NA
QAR74469.1\|63566_64736_-\|transposase	IS256 family transposase	NA	NA	NA	NA
QAR74470.1\|64897_65986_+	hypothetical protein	NA	NA	NA	NA
64816:64838	attR	NA	NA	NA	NA
QAR74471.1\|66126_67773_-\|transposase	IS1182 family transposase	A0ZS58	Staphylococcus_virus	3.0e-145	49.5
64816:64838	attR	NA	NA	NA	NA
QAR74472.1\|68054_68459_-	hypothetical protein	NA	NA	NA	NA
QAR74473.1\|68481_70308_-	hypothetical protein	NA	NA	NA	NA
QAR74474.1\|70482_70842_-	hypothetical protein	NA	NA	NA	NA
QAR74475.1\|71018_72174_+\|transposase	IS3 family transposase	A0A2I7SC85	Paenibacillus_phage	1.6e-79	51.3
QAR74476.1\|72158_72386_-	hypothetical protein	NA	NA	NA	NA
QAR74477.1\|72502_72607_-	resolvase	NA	NA	NA	NA
QAR74478.1\|72681_73446_-	iron export ABC transporter permease subunit FetB	NA	NA	NA	NA
QAR74479.1\|73442_74096_-	ATP-binding cassette domain-containing protein	G9BWD6	Planktothrix_phage	5.8e-23	36.0
QAR74480.1\|74179_74602_-	cupin domain-containing protein	Q2I8C5	Bacillus_phage	2.4e-38	58.5
QAR74481.1\|74670_74880_+	hypothetical protein	NA	NA	NA	NA
QAR74482.1\|76209_76404_-	hypothetical protein	NA	NA	NA	NA
QAR74483.1\|78312_78504_-	hypothetical protein	NA	NA	NA	NA
QAR74484.1\|78722_78959_-	hypothetical protein	NA	NA	NA	NA
QAR74485.1\|79529_79826_-\|bacteriocin	bacteriocin immunity protein	NA	NA	NA	NA
QAR74604.1\|79885_80428_-	ATP-binding cassette domain-containing protein	W8CYL7	Bacillus_phage	2.2e-23	42.0
QAR74486.1\|80510_81636_+\|transposase	IS3 family transposase	A0A1P8CWQ3	Bacillus_phage	8.6e-75	53.7
QAR74487.1\|82006_82420_+	Hsp20/alpha crystallin family protein	NA	NA	NA	NA
QAR74488.1\|82556_82817_+	hypothetical protein	NA	NA	NA	NA
QAR74489.1\|82934_83240_+	hypothetical protein	NA	NA	NA	NA
QAR74490.1\|83313_84273_+\|transposase	IS30 family transposase	W5R8L2	Staphylococcus_phage	2.5e-35	34.9
QAR74491.1\|84277_84541_-	hypothetical protein	NA	NA	NA	NA
QAR74492.1\|84545_84902_-	topoisomerase	NA	NA	NA	NA
QAR74493.1\|84901_85294_-	recombinase family protein	NA	NA	NA	NA
QAR74605.1\|85802_85988_+	hypothetical protein	NA	NA	NA	NA
QAR74494.1\|86104_87530_+\|transposase	IS3 family transposase	A0A1B1P773	Bacillus_phage	3.9e-48	40.2
QAR74495.1\|87825_88785_-\|transposase	IS30-like element IS6770 family transposase	W5R8L2	Staphylococcus_phage	6.1e-37	36.4
QAR74496.1\|88903_89866_-	LacI family transcriptional regulator	C6ZCU4	Enterobacteria_phage	3.0e-20	25.8
QAR74497.1\|89867_91307_-	sucrose-6-phosphate hydrolase	S6ATV4	Bacillus_phage	1.4e-16	25.1
QAR74498.1\|91491_93438_+	PTS beta-glucoside transporter subunit IIBCA	NA	NA	NA	NA
QAR74499.1\|93701_94574_+	ROK family protein	NA	NA	NA	NA
QAR74500.1\|96685_97639_+\|transposase	IS30 family transposase	W5R8L2	Staphylococcus_phage	1.6e-34	36.1
QAR74501.1\|97919_99089_-\|transposase	IS256 family transposase	NA	NA	NA	NA
QAR74502.1\|99308_101213_-	heavy metal translocating P-type ATPase	E4ZFI9	Streptococcus_phage	1.4e-98	35.0
QAR74503.1\|101410_101644_-	copper chaperone	NA	NA	NA	NA
QAR74504.1\|102630_102969_-	hypothetical protein	NA	NA	NA	NA
QAR74505.1\|103196_103901_-	Crp/Fnr family transcriptional regulator	NA	NA	NA	NA
QAR74506.1\|104045_104618_-	DNA starvation/stationary phase protection protein	A0A0A7RTZ1	Clostridium_phage	3.9e-23	42.6
QAR74507.1\|105153_106344_-\|transposase	IS256 family transposase	A0A0N9STL0	Staphylococcus_phage	4.3e-24	28.4
QAR74508.1\|107547_108045_-	GNAT family N-acetyltransferase	NA	NA	NA	NA
QAR74509.1\|108843_109047_-	transcriptional regulator	NA	NA	NA	NA
QAR74510.1\|109061_109451_-	DUF3796 domain-containing protein	NA	NA	NA	NA
QAR74511.1\|109452_110448_-	hypothetical protein	NA	NA	NA	NA
QAR74512.1\|110431_111115_-	ABC transporter ATP-binding protein	A0A2H4PQG7	Staphylococcus_phage	6.1e-15	27.4
QAR74513.1\|111699_112608_+\|transposase	IS982-like element ISEfm1 family transposase	NA	NA	NA	NA
QAR74514.1\|112995_113307_-	PadR family transcriptional regulator	NA	NA	NA	NA
QAR74515.1\|113855_114680_-	hydrolase	NA	NA	NA	NA
QAR74516.1\|114892_117055_-	PTS glucose/maltose transporter subunit IIBCA	A0A2I7SAJ6	Vibrio_phage	4.3e-06	41.1
QAR74517.1\|117335_119630_+	glycoside hydrolase family 65 protein	NA	NA	NA	NA
QAR74518.1\|119622_120288_+	beta-phosphoglucomutase	M1HXP3	Paramecium_bursaria_Chlorella_virus	2.5e-13	30.7
QAR74519.1\|120280_121315_+	galactose mutarotase	NA	NA	NA	NA
QAR74520.1\|122595_122916_-	hypothetical protein	NA	NA	NA	NA
QAR74521.1\|123195_123540_-	hypothetical protein	NA	NA	NA	NA
QAR74522.1\|123541_123697_-	DUF2087 domain-containing protein	NA	NA	NA	NA
QAR74523.1\|123936_124616_-\|transposase	IS6 family transposase	A0A1X9I6C6	Streptococcus_phage	7.9e-108	83.2
QAR74524.1\|124857_125058_+	hypothetical protein	NA	NA	NA	NA
QAR74525.1\|125263_126562_-	hypothetical protein	NA	NA	NA	NA
QAR74526.1\|126927_127836_+\|transposase	IS982 family transposase	NA	NA	NA	NA
QAR74527.1\|128298_129591_-	guanosine 5'-monophosphate oxidoreductase	NA	NA	NA	NA
QAR74606.1\|129565_130798_-	endonuclease	K4I1H4	Acidithiobacillus_phage	7.3e-19	36.1
QAR74528.1\|131176_131863_+\|transposase	IS6-like element ISEnfa1 family transposase	A0A1X9I6C6	Streptococcus_phage	4.8e-113	88.5
QAR74529.1\|132558_133623_-	2,3-butanediol dehydrogenase	E3SJ82	Synechococcus_phage	7.2e-15	27.7
QAR74530.1\|133909_135115_-	2-oxo acid dehydrogenase subunit E2	NA	NA	NA	NA
QAR74531.1\|135132_136164_-	alpha-ketoacid dehydrogenase subunit beta	NA	NA	NA	NA
QAR74532.1\|136188_137157_-	thiamine pyrophosphate-dependent dehydrogenase E1 component subunit alpha	NA	NA	NA	NA
QAR74533.1\|137173_138526_-	dihydrolipoyl dehydrogenase	A0A2K5B2C5	Erysipelothrix_phage	8.0e-43	30.2
QAR74534.1\|138879_140670_-	sigma-54-dependent Fis family transcriptional regulator	NA	NA	NA	NA
QAR74535.1\|140792_142094_-	DNA topoisomerase	A0A1X9I6W8	Streptococcus_phage	1.9e-105	99.0
QAR74536.1\|142093_142486_-	recombinase family protein	NA	NA	NA	NA
QAR74537.1\|142937_144077_-	MFS transporter	NA	NA	NA	NA
QAR74538.1\|144657_145248_-\|integrase	site-specific integrase	D2XR58	Bacillus_phage	8.3e-21	33.1
QAR74539.1\|145253_146087_-	GIY-YIG nuclease family protein	NA	NA	NA	NA
QAR74540.1\|146267_147245_-	toll/interleukin-1 receptor domain-containing protein	NA	NA	NA	NA
QAR74541.1\|147273_147540_-	hypothetical protein	A0A2H4PQP5	Staphylococcus_phage	6.2e-08	36.0
QAR74542.1\|147957_148317_-	XRE family transcriptional regulator	A0A1S5SFA6	Streptococcus_phage	4.4e-17	40.4
QAR74543.1\|148482_148680_+	DNA-binding protein	NA	NA	NA	NA
QAR74544.1\|148669_149284_+	XRE family transcriptional regulator	A0A139ZPI6	Marinitoga_camini_virus	5.5e-07	46.8
QAR74545.1\|149466_150384_+	ATP-binding cassette domain-containing protein	A0A2H4PQG7	Staphylococcus_phage	4.6e-42	43.2
QAR74546.1\|150376_151126_+	ABC transporter permease	NA	NA	NA	NA
QAR74547.1\|151125_151956_+	undecaprenyl-diphosphate phosphatase	NA	NA	NA	NA
QAR74548.1\|152381_152804_+	sigma-70 family RNA polymerase sigma factor	NA	NA	NA	NA
QAR74549.1\|154283_155440_-\|transposase	IS3 family transposase	A0A2I7SC85	Paenibacillus_phage	1.8e-75	51.3
QAR74550.1\|156610_157471_-	restriction endonuclease subunit S	NA	NA	NA	NA
QAR74551.1\|157460_159056_-	type I restriction-modification system subunit M	A0A2H4PQP4	Staphylococcus_phage	2.3e-126	51.1
QAR74552.1\|159179_159944_+	SinI family restriction endonuclease	NA	NA	NA	NA
QAR74553.1\|159957_160200_+	hypothetical protein	NA	NA	NA	NA
QAR74554.1\|161314_162487_-	hypothetical protein	A0A217EQY2	Bacillus_phage	2.6e-13	33.5
QAR74555.1\|162516_163245_-	peptidase	A0A1B0T6A2	Bacillus_phage	3.1e-09	26.1
QAR74556.1\|163504_164053_-	hypothetical protein	NA	NA	NA	NA
QAR74557.1\|164053_164911_-	ParA family protein	F0PIG8	Enterococcus_phage	8.1e-33	30.7
QAR74558.1\|165196_165484_-	type II toxin-antitoxin system RelE/ParE family toxin	NA	NA	NA	NA
QAR74559.1\|165473_165803_-	AbrB family transcriptional regulator	NA	NA	NA	NA
QAR74560.1\|165879_166152_-	Txe/YoeB family addiction module toxin	NA	NA	NA	NA
QAR74561.1\|166151_166415_-	type II toxin-antitoxin system Phd/YefM family antitoxin	NA	NA	NA	NA
QAR74562.1\|167559_168735_-	MFS transporter	NA	NA	NA	NA
QAR74563.1\|168737_170987_-	alpha-galactosidase	NA	NA	NA	NA
QAR74564.1\|171083_171938_-	AraC family transcriptional regulator	NA	NA	NA	NA
QAR74565.1\|172019_172928_-\|transposase	IS982-like element ISEfm1 family transposase	NA	NA	NA	NA
QAR74566.1\|173302_174262_-\|transposase	IS30 family transposase	W5R8L2	Staphylococcus_phage	1.6e-37	36.1
QAR74567.1\|175583_175901_-	type II toxin-antitoxin system RelE/ParE family toxin	NA	NA	NA	NA
QAR74568.1\|175901_176156_-	AbrB family transcriptional regulator	NA	NA	NA	NA
QAR74569.1\|178445_179486_+	replication protein RepA	NA	NA	NA	NA
QAR74570.1\|180309_180642_+	conjugal transfer protein TraE	NA	NA	NA	NA
QAR74571.1\|181260_181602_+	hypothetical protein	F0PIH7	Enterococcus_phage	3.1e-36	63.4
QAR74572.1\|181620_181911_+	hypothetical protein	NA	NA	NA	NA
QAR74573.1\|182102_182336_+	hypothetical protein	NA	NA	NA	NA
QAR74574.1\|182755_183427_+	class A sortase	NA	NA	NA	NA
QAR74575.1\|183479_185108_+	isopeptide-forming domain-containing fimbrial protein	NA	NA	NA	NA
QAR74576.1\|185616_186018_+\|transposase	IS200/IS605 family transposase	Q332K6	Clostridium_botulinum_C_phage	6.9e-43	62.4
QAR74577.1\|187603_188359_+	class C sortase	NA	NA	NA	NA
QAR74578.1\|188355_189183_+	hypothetical protein	NA	NA	NA	NA
QAR74607.1\|189192_189441_+	hypothetical protein	NA	NA	NA	NA
QAR74579.1\|189471_191583_+	isopeptide-forming domain-containing fimbrial protein	NA	NA	NA	NA
QAR74580.1\|191618_193670_-\|protease	serine protease	NA	NA	NA	NA

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

2. CP035136

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Crispr_ID: CP035136_1

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

CP035136_1

351729-352226

TypeII-A

II-A

Consensus_repeat	Method
GTTTTAGAGCTATGCTGATTTGAATGCTTCCAAAAC	PILER-CR
GTTTTAGAGCTATGCTGATTTGAATGCTTCCAAAAC	CRISPRCasFinder
GTTTTAGAGCTATGCTGATTTGAATGCTTCCAAAAC	CRT

7 spacers

csn2,cas2,cas1

The CRISPR arrays of CP035136_1

>merge|CP035136|1|351729-352226|PILER-CR,CRISPRCasFinder,CRT
GTTTTAGAGCTATGCTGATTTGAATGCTTCCAAAACTTTTTCATGCCATCAGTATGTCGTTGATGCGTTTTAGAGCTATGCTGATTTGAATGCTTCCAAAACCGTCATGTTCTACTACTACAGCACGTCCTTGTTTTAGAGCTATGCTGATTTGAATGCTTCCAAAACACATGGTTAAGTTGTATAGCGGAGTAACTGGTTTTAGAGCTATGCTGATTTGAATGCTTCCAAAACATGGAAGCATTAGTGTTGATCGATTTCGCCGTTTTAGAGCTATGCTGATTTGAATGCTTCCAAAACAAAAAATCGCTGGTAAGCTAGAAACTAGAGGTTTTAGAGCTATGCTGATTTGAATGCTTCCAAAACTGCTTTTATGTCTTTGCCTTCTTTAAACTGGTTTTAGAGCTATGCTGATTTGAATGCTTCCAAAGCATCTTTTTCCTACGATTTCTTTACCTGAAGGTTTTAGAGCTATGCTGATTTGAATACTACTCTAGA

>CP035136|1|1|351729-352160|PILER-CR
GTTTTAGAGCTATGCTGATTTGAATGCTTCCAAAAC	TTTTTCATGCCATCAGTATGTCGTTGATGC
GTTTTAGAGCTATGCTGATTTGAATGCTTCCAAAAC	CGTCATGTTCTACTACTACAGCACGTCCTT
GTTTTAGAGCTATGCTGATTTGAATGCTTCCAAAAC	ACATGGTTAAGTTGTATAGCGGAGTAACTG
GTTTTAGAGCTATGCTGATTTGAATGCTTCCAAAAC	ATGGAAGCATTAGTGTTGATCGATTTCGCC
GTTTTAGAGCTATGCTGATTTGAATGCTTCCAAAAC	AAAAAATCGCTGGTAAGCTAGAAACTAGAG
GTTTTAGAGCTATGCTGATTTGAATGCTTCCAAAAC	TGCTTTTATGTCTTTGCCTTCTTTAAACTG
GTTTTAGAGCTATGCTGATTTGAATGCTTCCAAAGC

>CP035136|1|1|351729-352226|CRISPRCasFinder
GTTTTAGAGCTATGCTGATTTGAATGCTTCCAAAAC	TTTTTCATGCCATCAGTATGTCGTTGATGC
GTTTTAGAGCTATGCTGATTTGAATGCTTCCAAAAC	CGTCATGTTCTACTACTACAGCACGTCCTT
GTTTTAGAGCTATGCTGATTTGAATGCTTCCAAAAC	ACATGGTTAAGTTGTATAGCGGAGTAACTG
GTTTTAGAGCTATGCTGATTTGAATGCTTCCAAAAC	ATGGAAGCATTAGTGTTGATCGATTTCGCC
GTTTTAGAGCTATGCTGATTTGAATGCTTCCAAAAC	AAAAAATCGCTGGTAAGCTAGAAACTAGAG
GTTTTAGAGCTATGCTGATTTGAATGCTTCCAAAAC	TGCTTTTATGTCTTTGCCTTCTTTAAACTG
GTTTTAGAGCTATGCTGATTTGAATGCTTCCAAAGC	ATCTTTTTCCTACGATTTCTTTACCTGAAG
GTTTTAGAGCTATGCTGATTTGAATACTACTCTAGA

>CP035136|1|1|351729-352226|CRT
GTTTTAGAGCTATGCTGATTTGAATGCTTCCAAAAC	TTTTTCATGCCATCAGTATGTCGTTGATGC
GTTTTAGAGCTATGCTGATTTGAATGCTTCCAAAAC	CGTCATGTTCTACTACTACAGCACGTCCTT
GTTTTAGAGCTATGCTGATTTGAATGCTTCCAAAAC	ACATGGTTAAGTTGTATAGCGGAGTAACTG
GTTTTAGAGCTATGCTGATTTGAATGCTTCCAAAAC	ATGGAAGCATTAGTGTTGATCGATTTCGCC
GTTTTAGAGCTATGCTGATTTGAATGCTTCCAAAAC	AAAAAATCGCTGGTAAGCTAGAAACTAGAG
GTTTTAGAGCTATGCTGATTTGAATGCTTCCAAAAC	TGCTTTTATGTCTTTGCCTTCTTTAAACTG
GTTTTAGAGCTATGCTGATTTGAATGCTTCCAAAGC	ATCTTTTTCCTACGATTTCTTTACCTGAAG
GTTTTAGAGCTATGCTGATTTGAATACTACTCTAGA

Protein	Signature genes	Signature genes Name	Protein_function
CP035136.1\|QAR72344.1\|352396_352762_+\|DUF1622-domain-containing-protein	unknown	unknown	gnl\|CDD\|377914
CP035136.1\|QAR72341.1\|345549_345747_+\|hypothetical-protein	unknown	unknown	unknown
CP035136.1\|QAR72342.1\|349778_350645_+\|type-II-CRISPR-associated-endonuclease-Cas1	cas1	cd09720_cas1_CAS-II-A:CAS-II-B:CAS-II-C	gnl\|CDD\|274694
CP035136.1\|QAR72350.1\|360109_360967_-\|LysR-family-transcriptional-regulator	unknown	unknown	gnl\|CDD\|223656
CP035136.1\|QAR72337.1\|340535_341669_-\|glutathione-dependent-formaldehyde-dehydrogenase	unknown	unknown	gnl\|CDD\|176243
CP035136.1\|QAR72339.1\|343353_345048_-\|oleate-hydratase	unknown	unknown	gnl\|CDD\|237575
CP035136.1\|QAR72340.1\|345104_345233_-\|methanol-dehydrogenase	unknown	unknown	unknown
CP035136.1\|QAR72347.1\|355417_357970_+\|HAD-family-hydrolase	unknown	unknown	gnl\|CDD\|319775
CP035136.1\|QAR74340.1\|350975_351638_+\|type-II-A-CRISPR-associated-protein-Csn2	csn2	cd09758_csn2_CAS-II-A	gnl\|CDD\|213408
CP035136.1\|QAR72348.1\|357976_359416_-\|HTH-domain-containing-protein	unknown	unknown	gnl\|CDD\|375760
CP035136.1\|QAR72352.1\|362004_363651_+\|malonate-decarboxylase-subunit-alpha	unknown	unknown	gnl\|CDD\|379906
CP035136.1\|QAR72343.1\|350641_350986_+\|CRISPR-associated-endonuclease-Cas2	cas2	COG3512_cas2_CAS-II-A:CAS-II-B:CAS-II-C	gnl\|CDD\|226043
CP035136.1\|QAR72351.1\|361086_361998_+\|ACP-S-malonyltransferase	unknown	unknown	gnl\|CDD\|132175
CP035136.1\|QAR72338.1\|341959_342820_+\|hypothetical-protein	unknown	unknown	unknown
CP035136.1\|QAR72345.1\|352758_353124_+\|DUF1622-domain-containing-protein	unknown	unknown	gnl\|CDD\|377914
CP035136.1\|QAR72346.1\|353284_354712_-\|DNA-binding-protein	unknown	unknown	gnl\|CDD\|311953
CP035136.1\|QAR72353.1\|363752_364052_+\|malonate-decarboxylase-subunit-delta	unknown	unknown	gnl\|CDD\|188283
CP035136.1\|QAR72336.1\|340190_340505_-\|hypothetical-protein	unknown	unknown	gnl\|CDD\|372928
CP035136.1\|QAR72349.1\|359559_359922_+\|hypothetical-protein	unknown	unknown	gnl\|CDD\|225197
CP035136.1\|QAR72335.1\|340003_340207_-\|hypothetical-protein	unknown	unknown	unknown

Protein	Function_ID	Function_description	E-value
CP035136.1\|QAR72344.1\|352396_352762_+\|DUF1622-domain-containing-protein	gnl\|CDD\|377914	pfam07784, DUF1622, Protein of unknown function (DUF1622). This is a family of 14 highly conserved sequences, from hypothetical proteins expressed by both bacterial and archaeal species.	1.12042e-21
CP035136.1\|QAR72339.1\|343353_345048_-\|oleate-hydratase	gnl\|CDD\|237575	PRK13977, PRK13977, myosin-cross-reactive antigen; Provisional.	0
CP035136.1\|QAR72342.1\|349778_350645_+\|type-II-CRISPR-associated-endonuclease-Cas1	gnl\|CDD\|274694	TIGR03639, cas1_NMENI, CRISPR-associated endonuclease Cas1, subtype II/NMENI. The CRISPR-associated protein Cas1 is virtually universal to CRISPR systems. CRISPR, an acronym for Clustered Regularly Interspaced Short Palindromic Repeats, is a prokaryotic immunity system for foreign DNA, mostly from phage. CRISPR systems belong to different subtypes, distinguished by both nature of the repeats, the makeup of the cohort of associated Cas proteins, and by molecular phylogeny within the more universal Cas proteins such as this one. This model is of type EXCEPTION and provides more specific information than the EQUIVALOG model TIGR00287. It describes the Cas1 variant of the NMENI subtype of CRISPR/Cas system.	1.91124e-120
CP035136.1\|QAR72337.1\|340535_341669_-\|glutathione-dependent-formaldehyde-dehydrogenase	gnl\|CDD\|176243	cd08283, FDH_like_1, Glutathione-dependent formaldehyde dehydrogenase related proteins, child 1. Members identified as glutathione-dependent formaldehyde dehydrogenase(FDH), a member of the zinc-dependent/medium chain alcohol dehydrogenase family. FDH converts formaldehyde and NAD(P) to formate and NAD(P)H. The initial step in this process the spontaneous formation of a S-(hydroxymethyl)glutathione adduct from formaldehyde and glutathione, followed by FDH-mediated oxidation (and detoxification) of the adduct to S-formylglutathione. MDH family uses NAD(H) as a cofactor in the interconversion of alcohols and aldehydes, or ketones. Like many zinc-dependent alcohol dehydrogenases (ADH) of the medium chain alcohol dehydrogenase/reductase family (MDR), these FDHs form dimers, with 4 zinc ions per dimer. The medium chain alcohol dehydrogenase family (MDR) has a NAD(P)(H)-binding domain in a Rossmann fold of a beta-alpha form. The N-terminal region typically has an all-beta catalytic domain. These proteins typically form dimers (typically higher plants, mammals) or tetramers (yeast, bacteria), and have 2 tightly bound zinc atoms per subunit.	1.57106e-174
CP035136.1\|QAR72352.1\|362004_363651_+\|malonate-decarboxylase-subunit-alpha	gnl\|CDD\|379906	pfam16957, Mal_decarbox_Al, Malonate decarboxylase, alpha subunit, transporter. Mal_decarbox_Al is a family of Na+-transporting carboxylic acid decarboxylases.	0
CP035136.1\|QAR72347.1\|355417_357970_+\|HAD-family-hydrolase	gnl\|CDD\|319775	cd02080, P-type_ATPase_cation, P-type cation-transporting ATPase similar to Exiguobacterium aurantiacum Mna, an Na(+)-ATPase, and Synechocystis sp. PCC 6803 PMA1, a putative Ca(2+)-ATPase. This subfamily includes the P-type Na(+)-ATPase of an alkaliphilic bacterium Exiguobacterium aurantiacum Mna and cyanobacterium Synechocystis sp. PCC 6803 PMA1, a cation-transporting ATPase which may translocate calcium. The P-type ATPases, are a large family of integral membrane transporters that are of critical importance in all kingdoms of life. They generate and maintain (electro-) chemical gradients across cellular membranes, by translocating cations, heavy metals and lipids, and are distinguished from other main classes of transport ATPases (F- , V- , and ABC- type) by the formation of a phosphorylated (P-) intermediate state in the catalytic cycle.	0
CP035136.1\|QAR74340.1\|350975_351638_+\|type-II-A-CRISPR-associated-protein-Csn2	gnl\|CDD\|213408	cd09758, Csn2, CRISPR/Cas system-associated protein Csn2. Csn2 is a Nmeni subtype-specific Cas protein, which may function in the adaptation process which mediates the incorporation of foreign nucleic acids into the microbial host genome. Csn 2 may interact directly with double-stranded DNA. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and associated Cas proteins comprise a system for heritable host defense by prokaryotic cells against phage and other foreign DNA. Csn2 has been predicted to be a functional analog of Cas4 based on anti-correlated phyletic patterns; also known as SPy1049 family.	1.01282e-107
CP035136.1\|QAR72348.1\|357976_359416_-\|HTH-domain-containing-protein	gnl\|CDD\|375760	pfam18333, ssDNA_DBD, Non-canonical single-stranded DNA-binding domain. This domain is found in ThermoDBP, a non-canonical single-stranded DNA-binding protein in Thermoproteales. Single-stranded DNA-binding proteins are needed for DNA metabolism, sequestering and protecting transiently formed ssDNA during DNA replication and recombination, detecting DNA damage and recruiting repair proteins. The outer edge of the ssDNA-binding cleft, formed by this domain, has a strongly positive electrostatic surface potential because of the conserved basic residues R49, K54, R65, R80, R86, R90, K97, and R112.	5.49729e-38
CP035136.1\|QAR72343.1\|350641_350986_+\|CRISPR-associated-endonuclease-Cas2	gnl\|CDD\|226043	COG3512, COG3512, CRISPR-associated protein, Cas2 homolog [Defense mechanisms].	9.51394e-45
CP035136.1\|QAR72351.1\|361086_361998_+\|ACP-S-malonyltransferase	gnl\|CDD\|132175	TIGR03131, malonate_mdcH, malonate decarboxylase, epsilon subunit. Members of this protein family are the epsilon subunit of malonate decarboxylase. This subunit has malonyl-CoA/dephospho-CoA acyltransferase activity. Malonate decarboxylase may be a soluble enzyme, or linked to membrane subunits and active as a sodium pump. The epsilon subunit is closely related to the malonyl CoA-acyl carrier protein (ACP) transacylase family described by TIGR00128, but acts on an ACP subunit of malonate decarboxylase that has an unusual coenzyme A derivative as its prothetic group.	4.19278e-66
CP035136.1\|QAR72345.1\|352758_353124_+\|DUF1622-domain-containing-protein	gnl\|CDD\|377914	pfam07784, DUF1622, Protein of unknown function (DUF1622). This is a family of 14 highly conserved sequences, from hypothetical proteins expressed by both bacterial and archaeal species.	8.2154e-25
CP035136.1\|QAR72346.1\|353284_354712_-\|DNA-binding-protein	gnl\|CDD\|311953	pfam08280, HTH_Mga, M protein trans-acting positive regulator (MGA) HTH domain. Mga is a DNA-binding protein that activates the expression of several important virulence genes in group A streptococcus in response to changing environmental conditions.	0.00286938
CP035136.1\|QAR72353.1\|363752_364052_+\|malonate-decarboxylase-subunit-delta	gnl\|CDD\|188283	TIGR03130, malonate_delta, malonate decarboxylase acyl carrier protein. Members of this protein family are the acyl carrier protein, also called the delta subunit, of malonate decarboxylase. This subunit has the same covalently bound prosthetic group, derived from and similar to coenzyme A, as does citrate lyase, although this protein and the acyl carrier protein of citrate lyase do not show significant sequence similarity. Both malonyl and acetyl groups are transferred to the prosthetic group for catalysis.	5.72696e-47
CP035136.1\|QAR72336.1\|340190_340505_-\|hypothetical-protein	gnl\|CDD\|372928	pfam14145, YrhK, YrhK-like protein. The YrhK-like protein family includes the B. subtilis YrhK protein, which is functionally uncharacterized. Its expression is under the control of the motility sigma factor sigma-D. This domain family is found in bacteria, archaea and eukaryotes, and is approximately 60 amino acids in length.	1.95591e-14
CP035136.1\|QAR72349.1\|359559_359922_+\|hypothetical-protein	gnl\|CDD\|225197	COG2315, MmcQ, Uncharacterized protein conserved in bacteria [Function unknown].	2.50194e-20
CP035136.1\|QAR72350.1\|360109_360967_-\|LysR-family-transcriptional-regulator	gnl\|CDD\|223656	COG0583, LysR, Transcriptional regulator [Transcription].	5.03447e-40

>CP035136.1|QAR74340.1|350975_351638_+|type-II-A-CRISPR-associated-protein-Csn2
MNLNFPLLEQPLPLEKATFLIVEDVTVFAKVVKWFYQYSESHELKLFNDKYKELKESDLLIITDVLGFDINSASTLKLIYADLERQLNEKVEVKSMIDKLTATISELIGYELLDHELDLEGDEITVLELFKALGIKIETRSDTIFEKLLELLQVYKYLSKKKLLVLINVCSYLTEEEISEVQDYISLYHMEVLFVEPRSIKGIKQFVLDKDYFLNLENMV
>CP035136.1|QAR72343.1|350641_350986_+|CRISPR-associated-endonuclease-Cas2
MSYRYMRMILMFDMPTETADERKAYRKFRKFLLSEGFIMHQFSVYSKLLLNPTASQAMLKRLKSNNPKKGFVTLLTVTEKQFARMVYLSGERDTSVANSDKRVIYLGEDEDAES
>CP035136.1|QAR72342.1|349778_350645_+|type-II-CRISPR-associated-endonuclease-Cas1
MGWRTVVVNTHSKLAYVNNHLIFKNAEKTERLHLSEIDVLLLETTDITITTMLLKRLVDEKILVLFCDDKRLPVAKLLPFYSRHDSSLQLTRQLAWSPEKKGQVWTAIISQKIQNQSNFLQKQLFTEKAESIQELHEGLLEFDPTNREGHAARIYFQTLFGTKFTREENNEINAGLDYGYTLLLSMFAREIVKNGCMTQFGLKHANQFNDFNLASDFMEPFRPVIDEIVYRYRNCEFPVIKRRLFDLFTNKYSYGKKEMFLTNIVSDYTSKVIKVLNNETEGVPQFRI
>CP035136.1|QAR72341.1|345549_345747_+|hypothetical-protein
MILKSKAQKKRREQRCFKKADDGQSLILTCYIDLNATNIFIIAKITAFTIGVFVFSIMMSCWMKG
>CP035136.1|QAR72340.1|345104_345233_-|methanol-dehydrogenase
MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI
>CP035136.1|QAR72339.1|343353_345048_-|oleate-hydratase
MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSNDGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTAEEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLNNITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVNRTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKMTNTLTQEEEHVTVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGNPDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDVNMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSKVDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGEFVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE
>CP035136.1|QAR72338.1|341959_342820_+|hypothetical-protein
MKQIQNKWNKRIIVMISSVCLFCLTVMLCLISTLANQHFMLEQNMQSDYAKKVAAEINQEIQSYHYEIGAPAEALEKIVSEELVQKNIELFIRNRYEKEPVELIGEKEVEEKLQEIINTYKQEEAEDDPFASEENNLLIYMTSGIFRRGIQSIYLMSFIDRILTIRERMVSFLPVISSILLVLLSGVFYLYRMSWKKVLWSFAVMIGGAGFQLLSLLVSLYLYDMTSQLDIRTQALQHLLDTYILSFIQFFSVVAIVEVVIAAGLWWIGKRVNTEKKVFSKRNEYK
>CP035136.1|QAR72337.1|340535_341669_-|glutathione-dependent-formaldehyde-dehydrogenase
MKAVTWQGKQKMEIRNVDDPRILEPTDAIIRITATAICGSDLHLYHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMESQCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNNLPDEKVLFLSDILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKAANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGTISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYEMIAEGLFDPTQIITHSLPLEEAAQAYEIFDKKEDKNIKVVLKP
>CP035136.1|QAR72336.1|340190_340505_-|hypothetical-protein
MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLLTGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKRTRKKHGKAV
>CP035136.1|QAR72335.1|340003_340207_-|hypothetical-protein
MGRPFSLLHAFLLFLCCAYFAYCDSKKFLSIEQFFGFLLYMLFHVPTLTAAFGTLAQEFLHYPEPSR
>CP035136.1|QAR72344.1|352396_352762_+|DUF1622-domain-containing-protein
MFNTQLIGYLEWIIAILNICSIGILIAGIVLVLFKLFDWSHVKKNYAERNARNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVISYFLNQEIEDKEKQRRGEVG
>CP035136.1|QAR72345.1|352758_353124_+|DUF1622-domain-containing-protein
MTTELMQFLLPYFEKLVLGLDIFAICLLIWGVCLSIKDFLKYLFVHTEAKNDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIRTIISYFLNKEIGPVDVPNK
>CP035136.1|QAR72346.1|353284_354712_-|DNA-binding-protein
MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSYLLHVCKTTLLNDINNIKKEFEEQIVYTHREKDCYTLYFSEHIPRCKIMQQLTQNSLFLKTCLLYLEENEPDYLQLTECEFISVSKAYSLKKQVLAYFHDCGIEIDRYSPRFTEMERRLLLLNVAYRLGSFKSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQLCTVTIDPTFIEEIKKRPIYNYVESAWETTDFQTYYKKEEFAFILTLFNLCNYGCHSYQLIAEDFQQLHQVFIDNTPEIKELVATFETHFHQELFGNQPFERALIHLMRSAWDNYQLFMPEKFYLLNEEQTNLYKEVQTLFSSWAKRLPYDLRFNPNCMRAFVIELSGILRLTKERLTIYIVTNSDVHYLIYREALEAVTTFDFQVAPTIYASISDIKKYAQQSSNRVLCERTLYTPDAVQYENIIPISINTIERAIVSAVQNK
>CP035136.1|QAR72347.1|355417_357970_+|HAD-family-hydrolase
MNWYKLTVEDTLKNHQTSTEGLSQEERQKRLTDQGYNEIEARQQTKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEESLNGLKQLMGQEAIVLSNGMQVKIPAKELVKGDVIVLRAGYVVPADVRLIEVHDLLVEESILTGESEASEKNHSVLAAEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKINQALQSVEQQTTPLIKKINRLNKQIFQGILCLILFLVVFTTFRYGMDWHILLSAMIALVVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNEMTVVDVAAKEETRVLSIMKNCQELKTKEEQKIENLSGNPTELALIRHTETANLPLKKIEAKIPFSSEYKYMATMHTEEKGTVIYVKGAPEVLLAKSTLSYSEQEAWGQTATAFAKKGQRVLGFAYKKVDSKQELTHESLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKMITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSEEELTRQVPKVDVFARTTPEHKLRIVKALQKNGEIVGMTGDGVNDAPALKRADIGIAMGIKGSEVSKQAADMVLGDDNFHTIAKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALILNRPLPLSPVQILWVNMVTTITLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVALLIMVPAYLLAMQFEGQSLQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKSLFLSLGILALLQGMVIFLPIGQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREFSR
>CP035136.1|QAR72348.1|357976_359416_-|HTH-domain-containing-protein
MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTERTIFSDLQSIRSHLPEGWDIFSDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLFTKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFYHRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKANCRLDSSIHISSTLSSLYDSALKKLYLKEGIYLKDTELSFASFCFLESWNYNNNYGQEIARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLVLKYHESPILIEPLERQYHRFLETYDQQYAHLYQQKENLLNDLKKEMEINEPNYFLQLLSLLIQQTIFSIKPNLFNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDSLDIPIVYISSIPTKNELNRLTELTFKTFL
>CP035136.1|QAR72349.1|359559_359922_+|hypothetical-protein
MLTEKELIDHIDETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVLMSVPEEKIYDDASDHRIIDILDLKVDPELGGILRGKEGYFPAYHMNKEHWISVDLRKIEKLDELAGLIDESFKSTQ
>CP035136.1|QAR72350.1|360109_360967_-|LysR-family-transcriptional-regulator
MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELGVPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASVSIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAIPKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATLRSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAKTMREFYLGK
>CP035136.1|QAR72351.1|361086_361998_+|ACP-S-malonyltransferase
MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENYQDTVFIQLALLTKAVFYLDEMERNGIQPDIVAGHSIGAFCAAVACRSLTFENAAELVYHRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSIEGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLFDARCLYIKNTDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKFGVDKGIRTINLDQHGIDDATFLYQKWR
>CP035136.1|QAR72352.1|362004_363651_+|malonate-decarboxylase-subunit-alpha
MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLIAPGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKIDFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNLYTGFNTEETPTIVEAAAFKDGIVIVQVNEVVDKLPRVDIPGDWVDVVVKADQPYQLEALFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLKGKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSNRTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPAWQSLRNNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSEDTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDRLRDAGIVKLPEDLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS
>CP035136.1|QAR72353.1|363752_364052_+|malonate-decarboxylase-subunit-delta
MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKKESTVKICTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK

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Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity
CP035136_1	1.2\|351831\|30\|CP035136\|PILER-CR,CRISPRCasFinder,CRT	351831-351860	30	KJ010489	Enterococcus phage IME-EFm1, complete genome	27249-27278	2	0.933
CP035136_1	1.2\|351831\|30\|CP035136\|PILER-CR,CRISPRCasFinder,CRT	351831-351860	30	LC513943	Enterococcus phage vB_EfaS-DELF1 DNA, complete genome	21235-21264	3	0.9
CP035136_1	1.2\|351831\|30\|CP035136\|PILER-CR,CRISPRCasFinder,CRT	351831-351860	30	MT119359	Enterococcus phage heks, complete genome	27870-27899	5	0.833
CP035136_1	1.2\|351831\|30\|CP035136\|PILER-CR,CRISPRCasFinder,CRT	351831-351860	30	MK125140	Enterococcus phage Nonaheksakonda, complete genome	24429-24458	5	0.833
CP035136_1	1.6\|352095\|30\|CP035136\|PILER-CR,CRISPRCasFinder,CRT	352095-352124	30	NC_007713	Sodalis glossinidius str. 'morsitans' plasmid pSG1, complete sequence	3795-3824	5	0.833
CP035136_1	1.6\|352095\|30\|CP035136\|PILER-CR,CRISPRCasFinder,CRT	352095-352124	30	NZ_LN854558	Sodalis glossinidius str. 'morsitans' isolate B4 plasmid pSG1, complete sequence	3794-3823	5	0.833
CP035136_1	1.6\|352095\|30\|CP035136\|PILER-CR,CRISPRCasFinder,CRT	352095-352124	30	NC_007182	Sodalis glossinidius pSG1 plasmid from Glossina austeni	57719-57748	5	0.833
CP035136_1	1.6\|352095\|30\|CP035136\|PILER-CR,CRISPRCasFinder,CRT	352095-352124	30	NC_007183	Sodalis glossinidius pSG1 plasmid from Glossina palpalis palpalis	57719-57748	5	0.833
CP035136_1	1.6\|352095\|30\|CP035136\|PILER-CR,CRISPRCasFinder,CRT	352095-352124	30	MN642089	Klebsiella phage N1M2, complete genome	183123-183152	7	0.767
CP035136_1	1.7\|352161\|30\|CP035136\|CRISPRCasFinder,CRT	352161-352190	30	MT773555	Myoviridae sp. isolate BML_3 genomic sequence	53738-53767	7	0.767
CP035136_1	1.1\|351765\|30\|CP035136\|PILER-CR,CRISPRCasFinder,CRT	351765-351794	30	MG945191	UNVERIFIED: Microviridae sp. isolate 696-1711, complete genome	855-884	8	0.733
CP035136_1	1.7\|352161\|30\|CP035136\|CRISPRCasFinder,CRT	352161-352190	30	NZ_CP013276	Bacillus thuringiensis serovar israelensis strain AM65-52 plasmid pAM65-52-1-360K, complete sequence	185329-185358	8	0.733
CP035136_1	1.7\|352161\|30\|CP035136\|CRISPRCasFinder,CRT	352161-352190	30	NZ_CP039722	Bacillus thuringiensis strain BT-59 plasmid p1, complete sequence	322667-322696	8	0.733
CP035136_1	1.7\|352161\|30\|CP035136\|CRISPRCasFinder,CRT	352161-352190	30	NZ_CP009349	Bacillus thuringiensis HD1002 plasmid 1, complete sequence	261401-261430	8	0.733
CP035136_1	1.7\|352161\|30\|CP035136\|CRISPRCasFinder,CRT	352161-352190	30	NZ_CP009336	Bacillus thuringiensis strain HD1011 plasmid 1, complete sequence	164265-164294	8	0.733
CP035136_1	1.7\|352161\|30\|CP035136\|CRISPRCasFinder,CRT	352161-352190	30	NZ_CP045023	Bacillus thuringiensis strain JW-1 plasmid p1, complete sequence	185330-185359	8	0.733
CP035136_1	1.6\|352095\|30\|CP035136\|PILER-CR,CRISPRCasFinder,CRT	352095-352124	30	NZ_CP048308	Escherichia coli strain 9 plasmid p009_D, complete sequence	24892-24921	9	0.7
CP035136_1	1.6\|352095\|30\|CP035136\|PILER-CR,CRISPRCasFinder,CRT	352095-352124	30	NZ_CP039706	Clostridium butyricum strain 4-1 plasmid p-butyl_plas_4-1, complete sequence	674755-674784	9	0.7
CP035136_1	1.6\|352095\|30\|CP035136\|PILER-CR,CRISPRCasFinder,CRT	352095-352124	30	NZ_CP033246	Clostridium butyricum strain CFSA3987 plasmid pCFSA3987, complete sequence	441623-441652	9	0.7
CP035136_1	1.6\|352095\|30\|CP035136\|PILER-CR,CRISPRCasFinder,CRT	352095-352124	30	NZ_CP033248	Clostridium butyricum strain CFSA3989 plasmid pCFSA3989, complete sequence	441670-441699	9	0.7
CP035136_1	1.6\|352095\|30\|CP035136\|PILER-CR,CRISPRCasFinder,CRT	352095-352124	30	NZ_AP019717	Clostridium butyricum strain NBRC 13949 plasmid pCBU1, complete sequence	713086-713115	9	0.7
CP035136_1	1.6\|352095\|30\|CP035136\|PILER-CR,CRISPRCasFinder,CRT	352095-352124	30	NZ_CP039703	Clostridium butyricum strain 29-1 plasmid p-butyl_plas_29-1, complete sequence	16345-16374	9	0.7
CP035136_1	1.6\|352095\|30\|CP035136\|PILER-CR,CRISPRCasFinder,CRT	352095-352124	30	NZ_CP013238	Clostridium butyricum strain CDC_51208 plasmid pNPD4_2, complete sequence	140046-140075	9	0.7

1. spacer 1.2|351831|30|CP035136|PILER-CR,CRISPRCasFinder,CRT matches to KJ010489 (Enterococcus phage IME-EFm1, complete genome) position: , mismatch: 2, identity: 0.933

cgtcatgttctactactacagcacgtcctt	CRISPR spacer
cctcatgttctaccactacagcacgtcctt	Protospacer
* ***********.****************

2. spacer 1.2|351831|30|CP035136|PILER-CR,CRISPRCasFinder,CRT matches to LC513943 (Enterococcus phage vB_EfaS-DELF1 DNA, complete genome) position: , mismatch: 3, identity: 0.9

cgtcatgttctactactacagcacgtcctt	CRISPR spacer
cctcatgttctacaactaccgcacgtcctt	Protospacer
* *********** ***** **********

3. spacer 1.2|351831|30|CP035136|PILER-CR,CRISPRCasFinder,CRT matches to MT119359 (Enterococcus phage heks, complete genome) position: , mismatch: 5, identity: 0.833

cgtcatgttctactactacagcacgtcctt	CRISPR spacer
ctttgtgttctaccactactgcacgtcctt	Protospacer
* *..********.***** **********

4. spacer 1.2|351831|30|CP035136|PILER-CR,CRISPRCasFinder,CRT matches to MK125140 (Enterococcus phage Nonaheksakonda, complete genome) position: , mismatch: 5, identity: 0.833

cgtcatgttctactactacagcacgtcctt	CRISPR spacer
ctttgtgttctaccactactgcacgtcctt	Protospacer
* *..********.***** **********

5. spacer 1.6|352095|30|CP035136|PILER-CR,CRISPRCasFinder,CRT matches to NC_007713 (Sodalis glossinidius str. 'morsitans' plasmid pSG1, complete sequence) position: , mismatch: 5, identity: 0.833

tgcttttatgtctttgccttcttta--aactg	CRISPR spacer
ttcttttatggctttgtcttctttagtaac--	Protospacer
* ******** *****.********  ***

6. spacer 1.6|352095|30|CP035136|PILER-CR,CRISPRCasFinder,CRT matches to NZ_LN854558 (Sodalis glossinidius str. 'morsitans' isolate B4 plasmid pSG1, complete sequence) position: , mismatch: 5, identity: 0.833

tgcttttatgtctttgccttcttta--aactg	CRISPR spacer
ttcttttatggctttgtcttctttagtaac--	Protospacer
* ******** *****.********  ***

7. spacer 1.6|352095|30|CP035136|PILER-CR,CRISPRCasFinder,CRT matches to NC_007182 (Sodalis glossinidius pSG1 plasmid from Glossina austeni) position: , mismatch: 5, identity: 0.833

tgcttttatgtctttgccttcttta--aactg	CRISPR spacer
ttcttttatggctttgtcttctttagtaac--	Protospacer
* ******** *****.********  ***

8. spacer 1.6|352095|30|CP035136|PILER-CR,CRISPRCasFinder,CRT matches to NC_007183 (Sodalis glossinidius pSG1 plasmid from Glossina palpalis palpalis) position: , mismatch: 5, identity: 0.833

tgcttttatgtctttgccttcttta--aactg	CRISPR spacer
ttcttttatggctttgtcttctttagtaac--	Protospacer
* ******** *****.********  ***

9. spacer 1.6|352095|30|CP035136|PILER-CR,CRISPRCasFinder,CRT matches to MN642089 (Klebsiella phage N1M2, complete genome) position: , mismatch: 7, identity: 0.767

tgcttttatgtctttgccttctttaaactg	CRISPR spacer
gactattatgtctttgcctactttactatg	Protospacer
 .** ************** *****   **

10. spacer 1.7|352161|30|CP035136|CRISPRCasFinder,CRT matches to MT773555 (Myoviridae sp. isolate BML_3 genomic sequence) position: , mismatch: 7, identity: 0.767

atctttttcctacgatttctttacctgaag	CRISPR spacer
ttctttttcctacgaattctctaccgtcaa	Protospacer
 ************** ****.****   *.

11. spacer 1.1|351765|30|CP035136|PILER-CR,CRISPRCasFinder,CRT matches to MG945191 (UNVERIFIED: Microviridae sp. isolate 696-1711, complete genome) position: , mismatch: 8, identity: 0.733

tttttcatgccatcagtatgtcgttgatgc	CRISPR spacer
agttccatgccctcagtatgtcgttaacaa	Protospacer
  **.****** *************.*..

12. spacer 1.7|352161|30|CP035136|CRISPRCasFinder,CRT matches to NZ_CP013276 (Bacillus thuringiensis serovar israelensis strain AM65-52 plasmid pAM65-52-1-360K, complete sequence) position: , mismatch: 8, identity: 0.733

atctttttcctacgatttctttacctgaag	CRISPR spacer
atctttttcctactatttttttataaatat	Protospacer
************* ****.****.  . *

13. spacer 1.7|352161|30|CP035136|CRISPRCasFinder,CRT matches to NZ_CP039722 (Bacillus thuringiensis strain BT-59 plasmid p1, complete sequence) position: , mismatch: 8, identity: 0.733

atctttttcctacgatttctttacctgaag	CRISPR spacer
atctttttcctactatttttttataaatat	Protospacer
************* ****.****.  . *

14. spacer 1.7|352161|30|CP035136|CRISPRCasFinder,CRT matches to NZ_CP009349 (Bacillus thuringiensis HD1002 plasmid 1, complete sequence) position: , mismatch: 8, identity: 0.733

atctttttcctacgatttctttacctgaag	CRISPR spacer
atctttttcctactatttttttataaatat	Protospacer
************* ****.****.  . *

15. spacer 1.7|352161|30|CP035136|CRISPRCasFinder,CRT matches to NZ_CP009336 (Bacillus thuringiensis strain HD1011 plasmid 1, complete sequence) position: , mismatch: 8, identity: 0.733

atctttttcctacgatttctttacctgaag	CRISPR spacer
atctttttcctactatttttttataaatat	Protospacer
************* ****.****.  . *

16. spacer 1.7|352161|30|CP035136|CRISPRCasFinder,CRT matches to NZ_CP045023 (Bacillus thuringiensis strain JW-1 plasmid p1, complete sequence) position: , mismatch: 8, identity: 0.733

atctttttcctacgatttctttacctgaag	CRISPR spacer
atctttttcctactatttttttataaatat	Protospacer
************* ****.****.  . *

17. spacer 1.6|352095|30|CP035136|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP048308 (Escherichia coli strain 9 plasmid p009_D, complete sequence) position: , mismatch: 9, identity: 0.7

tgcttttatgtctttgccttctttaaactg	CRISPR spacer
cagttttttggctttgccttctttaatgat	Protospacer
.. **** ** ***************

18. spacer 1.6|352095|30|CP035136|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP039706 (Clostridium butyricum strain 4-1 plasmid p-butyl_plas_4-1, complete sequence) position: , mismatch: 9, identity: 0.7

tgcttttatgtctttgccttctttaaactg	CRISPR spacer
atattttatttcttttccttctttaacagt	Protospacer
   ****** ***** **********

19. spacer 1.6|352095|30|CP035136|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP033246 (Clostridium butyricum strain CFSA3987 plasmid pCFSA3987, complete sequence) position: , mismatch: 9, identity: 0.7

tgcttttatgtctttgccttctttaaactg	CRISPR spacer
atattttatttcttttccttctttaacagt	Protospacer
   ****** ***** **********

20. spacer 1.6|352095|30|CP035136|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP033248 (Clostridium butyricum strain CFSA3989 plasmid pCFSA3989, complete sequence) position: , mismatch: 9, identity: 0.7

tgcttttatgtctttgccttctttaaactg	CRISPR spacer
atattttatttcttttccttctttaacagt	Protospacer
   ****** ***** **********

21. spacer 1.6|352095|30|CP035136|PILER-CR,CRISPRCasFinder,CRT matches to NZ_AP019717 (Clostridium butyricum strain NBRC 13949 plasmid pCBU1, complete sequence) position: , mismatch: 9, identity: 0.7

tgcttttatgtctttgccttctttaaactg	CRISPR spacer
atattttatttcttttccttctttaacagt	Protospacer
   ****** ***** **********

22. spacer 1.6|352095|30|CP035136|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP039703 (Clostridium butyricum strain 29-1 plasmid p-butyl_plas_29-1, complete sequence) position: , mismatch: 9, identity: 0.7

tgcttttatgtctttgccttctttaaactg	CRISPR spacer
atattttatttcttttccttctttaacagt	Protospacer
   ****** ***** **********

23. spacer 1.6|352095|30|CP035136|PILER-CR,CRISPRCasFinder,CRT matches to NZ_CP013238 (Clostridium butyricum strain CDC_51208 plasmid pNPD4_2, complete sequence) position: , mismatch: 9, identity: 0.7

tgcttttatgtctttgccttctttaaactg	CRISPR spacer
atattttatttcttttccttctttaacagt	Protospacer
   ****** ***** **********

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

269477 : 299374

Clostridium_phage(11.11%)

protease,transposase

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
QAR72276.1\|269477_270386_+\|transposase	IS982-like element ISEfm1 family transposase	NA	NA	NA	NA
QAR72277.1\|270441_270939_+	GNAT family N-acetyltransferase	NA	NA	NA	NA
QAR72278.1\|270975_271671_-	hypothetical protein	NA	NA	NA	NA
QAR72279.1\|272501_273461_+\|transposase	IS30-like element IS6770 family transposase	NA	NA	NA	NA
QAR74336.1\|273781_274219_+	hypothetical protein	NA	NA	NA	NA
QAR72280.1\|274215_274839_-	recombinase family protein	M9Q1K0	Clostridium_phage	1.7e-16	33.0
QAR72281.1\|275132_275495_+	recombinase family protein	NA	NA	NA	NA
QAR74337.1\|276509_276977_+	PTS sugar transporter subunit IIA	NA	NA	NA	NA
QAR72282.1\|276966_277248_+	PTS sugar transporter subunit IIB	NA	NA	NA	NA
QAR72283.1\|277769_278381_+	SIS domain-containing protein	A0A1V0DXF5	Yersinia_phage	2.4e-15	31.5
QAR72284.1\|278394_279297_+	dihydrodipicolinate synthase family protein	NA	NA	NA	NA
QAR72285.1\|279299_279926_+	bifunctional 2-keto-4-hydroxyglutarate aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase	NA	NA	NA	NA
QAR72286.1\|279950_282035_+	transcription antiterminator	NA	NA	NA	NA
QAR72287.1\|282031_282754_+	HAD family phosphatase	A0A1D8KNV9	Synechococcus_phage	2.4e-09	25.5
QAR72288.1\|282776_284202_+\|transposase	IS3 family transposase	A0A1B1P773	Bacillus_phage	8.7e-48	40.2
QAR72289.1\|285532_285736_-	hypothetical protein	NA	NA	NA	NA
QAR72290.1\|285977_287246_-	PTS sugar transporter subunit IIC	NA	NA	NA	NA
QAR72291.1\|287268_287547_-	PTS sugar transporter subunit IIB	NA	NA	NA	NA
QAR72292.1\|288119_288800_+\|transposase	IS6-like element ISS1N family transposase	A0A1X9I6C6	Streptococcus_phage	2.5e-109	84.1
QAR72293.1\|288887_289232_-	hypothetical protein	NA	NA	NA	NA
QAR72294.1\|289312_289816_-	peptidase	A0A1B2APZ3	Phage_Wrath	2.2e-38	54.4
QAR72295.1\|289778_290015_+	XRE family transcriptional regulator	A0A097PBE6	Streptococcus_pyogenes_phage	5.7e-13	52.1
QAR72296.1\|290251_292366_+\|protease	ATP-dependent Clp protease ATP-binding subunit	K4FB40	Cronobacter_phage	2.4e-118	39.1
QAR74338.1\|292942_293170_+	hypothetical protein	NA	NA	NA	NA
QAR72297.1\|293388_293580_+	hypothetical protein	NA	NA	NA	NA
QAR72298.1\|295189_297433_+	LPXTG cell wall anchor domain-containing protein	NA	NA	NA	NA
QAR72299.1\|297729_298179_-	hypothetical protein	NA	NA	NA	NA
QAR72300.1\|298480_299374_-\|protease	metalloprotease	A0A1I9SA48	Rhodococcus_phage	7.1e-32	34.8

DBSCAN-SWA_2

968202 : 1045204

Enterococcus_phage(47.5%)

tail,transposase,terminase,holin,tRNA,head,portal,capsid,protease,integrase

attL 988215:988236|attR 1024675:1024696

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
QAR74361.1\|968202_968955_+\|tRNA	tRNA (guanosine(37)-N1)-methyltransferase TrmD	NA	NA	NA	NA
QAR72896.1\|969098_969446_+	50S ribosomal protein L19	NA	NA	NA	NA
QAR72897.1\|969702_969996_+	DUF2087 domain-containing protein	NA	NA	NA	NA
QAR72898.1\|970083_970740_-\|protease	CPBP family intramembrane metalloprotease	NA	NA	NA	NA
QAR74362.1\|973149_973410_+	LPXTG cell wall anchor domain-containing protein	NA	NA	NA	NA
QAR72899.1\|973966_974908_+	isopeptide-forming domain-containing fimbrial protein	NA	NA	NA	NA
QAR74363.1\|975519_976335_+	LPXTG cell wall anchor domain-containing protein	NA	NA	NA	NA
QAR72900.1\|977958_978156_+	hypothetical protein	NA	NA	NA	NA
QAR74364.1\|978123_978618_-	hypothetical protein	NA	NA	NA	NA
QAR72901.1\|979206_979629_+	HTH domain-containing protein	NA	NA	NA	NA
QAR72902.1\|979794_980988_-	MFS transporter	NA	NA	NA	NA
QAR74365.1\|981184_981556_+	hypothetical protein	NA	NA	NA	NA
QAR72903.1\|983053_984718_+	acetolactate synthase AlsS	NA	NA	NA	NA
QAR72904.1\|984730_985441_+	acetolactate decarboxylase	NA	NA	NA	NA
QAR72905.1\|985569_986067_+	GAF domain-containing protein	NA	NA	NA	NA
QAR72906.1\|986305_986512_+	hypothetical protein	NA	NA	NA	NA
QAR72907.1\|986923_987460_-	VanZ family protein	NA	NA	NA	NA
988215:988236	attL	NA	NA	NA	NA
QAR72908.1\|988241_989384_-\|integrase	site-specific integrase	M1PRU8	Streptococcus_phage	1.1e-56	36.9
QAR74366.1\|989535_989751_+	hypothetical protein	NA	NA	NA	NA
QAR72909.1\|989726_990725_-	hypothetical protein	NA	NA	NA	NA
QAR72910.1\|990830_991037_-	DUF4177 domain-containing protein	NA	NA	NA	NA
QAR74367.1\|991060_991162_-	LysM domain-containing protein	NA	NA	NA	NA
QAR72911.1\|991431_992103_-	DUF5067 domain-containing protein	NA	NA	NA	NA
QAR72912.1\|992235_992889_-	hypothetical protein	NA	NA	NA	NA
QAR72913.1\|992938_993367_-	ImmA/IrrE family metallo-endopeptidase	NA	NA	NA	NA
QAR72914.1\|993371_993746_-	XRE family transcriptional regulator	O03970	Lactobacillus_phage	3.2e-18	38.7
QAR72915.1\|994049_994235_+	XRE family transcriptional regulator	Q5YAA3	Bacillus_phage	5.4e-11	53.3
QAR72916.1\|994247_994475_+	DNA-binding protein	NA	NA	NA	NA
QAR72917.1\|994825_995533_+	phage regulatory protein	D2IYT0	Enterococcus_phage	8.9e-70	56.9
QAR72918.1\|995710_996052_+	hypothetical protein	NA	NA	NA	NA
QAR72919.1\|996044_996779_+	DUF1071 domain-containing protein	A0A1X9IGE5	Lactococcus_phage	2.1e-42	48.4
QAR72920.1\|997474_998224_+	helix-turn-helix domain-containing protein	A0A1L2BY83	Clostridium_phage	1.7e-31	54.0
QAR72921.1\|998235_998505_+	hypothetical protein	NA	NA	NA	NA
QAR72922.1\|998666_998966_+	hypothetical protein	NA	NA	NA	NA
QAR72923.1\|998965_999283_+	DUF1140 family protein	A0A2H4JAZ4	uncultured_Caudovirales_phage	3.0e-09	35.4
QAR72924.1\|999279_999630_+	DUF1642 domain-containing protein	A0A126GGJ0	Streptococcus_phage	5.3e-07	36.7
QAR72925.1\|999803_1000058_-	hypothetical protein	NA	NA	NA	NA
QAR74368.1\|1000301_1000715_+	autolysin	C9E2P5	Enterococcus_phage	1.1e-56	81.0
QAR72926.1\|1001245_1001458_+	hypothetical protein	D2IZF7	Enterococcus_phage	3.8e-08	59.6
QAR74369.1\|1001464_1001692_+	hypothetical protein	NA	NA	NA	NA
QAR72927.1\|1001696_1001897_+	hypothetical protein	NA	NA	NA	NA
QAR72928.1\|1001889_1002264_+	HNH endonuclease	A0A249XUN4	Enterococcus_phage	1.1e-34	57.6
QAR72929.1\|1002430_1002910_+\|terminase	P27 family phage terminase small subunit	A0A0M3UL59	Enterococcus_phage	1.0e-40	57.7
QAR72930.1\|1002902_1004627_+\|terminase	terminase large subunit	D7PQ39	Enterococcus_phage	2.2e-170	53.8
QAR72931.1\|1004800_1005985_+\|portal	phage portal protein	V5US36	Enterococcus_phage	6.7e-78	43.1
QAR74370.1\|1005932_1006478_+\|head,protease	HK97 family phage prohead protease	A0A0M4R4P2	Enterococcus_phage	4.8e-55	59.8
QAR72932.1\|1006517_1007714_+\|capsid	phage major capsid protein	A0A0S2SXJ6	Bacillus_phage	1.3e-81	55.3
QAR72933.1\|1007758_1007968_+	hypothetical protein	A5GYM0	Lactococcus_phage	4.2e-12	61.2
QAR72934.1\|1007979_1008264_+\|head,tail	phage gp6-like head-tail connector protein	A0A0M4S687	Enterococcus_phage	2.5e-15	45.7
QAR72935.1\|1008241_1008577_+\|head,tail	head-tail adaptor protein	C0LZZ3	Enterococcus_phage	1.0e-15	37.8
QAR72936.1\|1008573_1008969_+	hypothetical protein	A0A0M5M1E5	Enterococcus_phage	5.8e-18	41.7
QAR72937.1\|1008965_1009337_+	hypothetical protein	A0A060ANH4	Enterococcus_phage	8.6e-24	47.0
QAR72938.1\|1009352_1009913_+\|tail	phage tail protein	V5UQL2	Enterococcus_phage	1.2e-40	46.2
QAR72939.1\|1010020_1010332_+	hypothetical protein	A0A0M4R2F4	Enterococcus_phage	2.3e-14	46.7
QAR72940.1\|1010355_1010547_+	hypothetical protein	NA	NA	NA	NA
QAR72941.1\|1010546_1014410_+\|tail	phage tail protein	A0A0M5M3L4	Enterococcus_phage	0.0e+00	66.4
QAR72942.1\|1014420_1015251_+\|tail	phage tail family protein	A8ASK7	Listeria_phage	9.8e-52	40.8
QAR72943.1\|1015259_1016279_+	hypothetical protein	Q9T1A5	Listeria_phage	1.3e-106	55.3
QAR72944.1\|1016278_1017325_+	hypothetical protein	A0A059T661	Listeria_phage	1.2e-17	32.0
QAR72945.1\|1017324_1019598_+	DUF2479 domain-containing protein	A0A096XSZ6	Enterococcus_phage	9.5e-73	45.2
QAR72946.1\|1019616_1020063_+	hypothetical protein	NA	NA	NA	NA
QAR72947.1\|1020064_1020202_+	XkdX family protein	NA	NA	NA	NA
QAR74371.1\|1020236_1020479_+\|holin	holin	D2J075	Enterococcus_phage	2.0e-21	62.0
QAR72948.1\|1020494_1020692_+\|holin	holin	A0A0S2MYF6	Enterococcus_phage	5.9e-24	87.5
QAR72949.1\|1020702_1021722_+	N-acetylmuramoyl-L-alanine amidase	A0A1G5SA77	Enterococcus_phage	1.9e-60	66.1
QAR72950.1\|1022038_1022617_+	hypothetical protein	NA	NA	NA	NA
QAR72951.1\|1022613_1022895_+	hypothetical protein	NA	NA	NA	NA
QAR72952.1\|1022894_1023344_+	hypothetical protein	NA	NA	NA	NA
QAR72953.1\|1024269_1024572_-	hypothetical protein	NA	NA	NA	NA
QAR72954.1\|1025329_1026103_-	Cof-type HAD-IIB family hydrolase	NA	NA	NA	NA
1024675:1024696	attR	NA	NA	NA	NA
QAR72955.1\|1026246_1026597_+	SdpI family protein	NA	NA	NA	NA
QAR72956.1\|1026577_1027294_+	response regulator transcription factor	W8CYM9	Bacillus_phage	6.1e-34	36.2
QAR72957.1\|1027293_1028742_+	HAMP domain-containing histidine kinase	A0A1V0SGX0	Hokovirus	1.0e-19	29.3
QAR72958.1\|1028814_1029324_-	hypothetical protein	NA	NA	NA	NA
QAR72959.1\|1029313_1030039_-	FtsX-like permease family protein	NA	NA	NA	NA
QAR72960.1\|1030210_1032331_-	LTA synthase family protein	W6LM83	Streptococcus_phage	5.7e-221	58.4
QAR72961.1\|1032559_1033738_+	class I SAM-dependent rRNA methyltransferase	W6LLI2	Streptococcus_phage	2.1e-103	49.7
QAR72962.1\|1033753_1034512_+	type I 3-dehydroquinate dehydratase	W6LP76	Streptococcus_phage	2.5e-25	35.6
QAR72963.1\|1034534_1035020_+\|tRNA	Cys-tRNA(Pro) deacylase	NA	NA	NA	NA
QAR72964.1\|1035090_1036344_-	NAD(P)/FAD-dependent oxidoreductase	A0A2H4PQX1	Staphylococcus_phage	4.5e-24	55.3
QAR72965.1\|1036460_1037810_+	NADP-specific glutamate dehydrogenase	NA	NA	NA	NA
QAR72966.1\|1037921_1039268_+	glucose-6-phosphate isomerase	NA	NA	NA	NA
QAR72967.1\|1040749_1041418_-	hypothetical protein	Q9AZW5	Lactococcus_phage	2.0e-39	50.3
QAR72968.1\|1041482_1041911_-	ImmA/IrrE family metallo-endopeptidase	NA	NA	NA	NA
QAR72969.1\|1041917_1042298_-	XRE family transcriptional regulator	O03970	Lactobacillus_phage	2.8e-17	34.9
QAR72970.1\|1042461_1042713_+	XRE family transcriptional regulator	NA	NA	NA	NA
QAR72971.1\|1042925_1043480_+	hypothetical protein	NA	NA	NA	NA
QAR72972.1\|1043476_1043707_+	DNA-binding protein	NA	NA	NA	NA
QAR72973.1\|1043723_1043909_+	hypothetical protein	NA	NA	NA	NA
QAR72974.1\|1044042_1045204_+\|transposase	IS3 family transposase	A0A2I7SC85	Paenibacillus_phage	1.6e-79	51.3

DBSCAN-SWA_3

1944955 : 1953427

Streptococcus_phage(66.67%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
QAR73789.1\|1944955_1947145_+	anaerobic ribonucleoside-triphosphate reductase	A0A0C5KKX3	Enterococcus_phage	6.8e-286	66.7
QAR73790.1\|1947238_1947442_+	hypothetical protein	NA	NA	NA	NA
QAR73791.1\|1947459_1948062_+	anaerobic ribonucleoside-triphosphate reductase activating protein	A0A288TZV3	Enterococcus_phage	4.9e-53	58.0
QAR73792.1\|1948115_1949237_-	DNA polymerase IV	NA	NA	NA	NA
QAR73793.1\|1949345_1950218_-	16S rRNA (cytidine(1402)-2'-O)-methyltransferase	M1PLC5	Streptococcus_phage	2.1e-89	60.1
QAR73794.1\|1950286_1950634_-	DNA replication initiation control protein YabA	M1PFV3	Streptococcus_phage	5.1e-18	41.1
QAR73795.1\|1950626_1951451_-	signal peptidase	NA	NA	NA	NA
QAR73796.1\|1951486_1952425_-	DNA polymerase III subunit delta'	M1NSC1	Streptococcus_phage	6.1e-34	33.8
QAR73797.1\|1952438_1952768_-	hypothetical protein	NA	NA	NA	NA
QAR73798.1\|1952782_1953427_-	dTMP kinase	M1PSC7	Streptococcus_phage	4.2e-58	52.6

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

Overview of predicted results

Overview of the results

Cas Category Instructions

Results visualization

1. CP035137

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection

2. CP035136

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Click the colored protein region to show detailed information

Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection