CRISPRimmunity

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Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Self targeting spacer number	Target MGE spacer number	Prophage number	Anti-CRISPR protein number
LT992492	Vibrio cholerae strain 4295STDY6534248 genome assembly, chromosome: 1	1 crisprs	cas3,RT,DEDDh,DinG,csx1,csa3,WYL	0	1	5	0
LT992493	Vibrio cholerae strain 4295STDY6534248 genome assembly, chromosome: 2	0 crisprs	csa3,cas3	0	0	1	0

Results visualization

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CRISPR-Cas detection and classification

Crispr_ID: LT992492_1

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

LT992492_1

2660179-2660422

Orphan

Consensus_repeat	Method
AAAATGTGCCTCCTTAGCTCAGTCGGTAGAGCAACGGACTGATAATCCG	PILER-CR

2 spacers

The CRISPR arrays of LT992492_1

>merge|LT992492|1|2660179-2660422|PILER-CR
TGTGCCGGCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCGCCAGTTCGATTCCGGCAGCCGGCACCACTTAAAATTTGCCTCGATAGCTCAGTCGGTAGAGCAGAGGATTGAAAATCCTCGTGTCGGTGGTTCGATTCCGCCTCGAGGCACCACTATTAAAAGTTGTGCCATAGACACAATAAAAATTGTTCCTCCTTAGCTCAGTCGGTAGAGCGACGGACTGTTAATCCG

>LT992492|1|1|2660179-2660422|PILER-CR
TGTGCCGGCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGG	TCGCCAGTTCGATTCCGGCAGCCGGCACCACTTAAAA
TTTGCCTCGATAGCTCAGTCGGTAGAGCAGAGGATTGAAAATCCTCGTG	TCGGTGGTTCGATTCCGCCTCGAGGCACCACTATTAAAAGTTGTGCCATAGACACAATAAAAAT
TGTTCCTCCTTAGCTCAGTCGGTAGAGCGACGGACTGTTAATCCG

Protein	Signature genes	Signature genes Name	Protein_function
LT992492.1\|SPM22337.1\|2670369_2671179_+\|diadenosine-tetraphosphatase	unknown	unknown	gnl\|CDD\|234673
LT992492.1\|SPM22325.1\|2655381_2656302_-\|glutaminase	unknown	unknown	gnl\|CDD\|234880
LT992492.1\|SPM22320.1\|2651995_2652553_+\|Integral-membrane-protein-YggT	unknown	unknown	gnl\|CDD\|223833
LT992492.1\|SPM22322.1\|2652888_2653320_+\|Uncharacterised-protein	unknown	unknown	gnl\|CDD\|379616
LT992492.1\|SPM22329.1\|2658820_2659948_+\|membrane-bound-lytic-murein-transglycosylase-C	unknown	unknown	gnl\|CDD\|183271
LT992492.1\|SPM22333.1\|2666800_2668096_+\|survival-protein-SurA	unknown	unknown	gnl\|CDD\|236758
LT992492.1\|SPM22330.1\|2660711_2662337_+\|methyl-accepting-chemotaxis-protein	unknown	unknown	gnl\|CDD\|223910
LT992492.1\|SPM22328.1\|2658446_2658719_+\|oxidative-damage-protection-protein	unknown	unknown	gnl\|CDD\|180059
LT992492.1\|SPM22321.1\|2652552_2652843_+\|UPF0235-protein	unknown	unknown	gnl\|CDD\|179931
LT992492.1\|SPM22332.1\|2664396_2666748_+\|organic-solvent-tolerance-protein	unknown	unknown	gnl\|CDD\|235158
LT992492.1\|SPM22331.1\|2663383_2664238_-\|Dna-J-like-membrane-chaperone-protein	unknown	unknown	gnl\|CDD\|236512
LT992492.1\|SPM22327.1\|2657371_2658433_+\|A/G-specific-adenine-glycosylase	unknown	unknown	gnl\|CDD\|182805
LT992492.1\|SPM22326.1\|2656412_2657132_-\|tRNA-(guanine-N(7)-)-methyltransferase	unknown	unknown	gnl\|CDD\|234649
LT992492.1\|SPM22335.1\|2669091_2669907_+\|dimethyladenosine-transferase	unknown	unknown	gnl\|CDD\|234708
LT992492.1\|SPM22336.1\|2669978_2670359_+\|CO2+/MG2+-efflux-protein-ApaG	unknown	unknown	gnl\|CDD\|180098
LT992492.1\|SPM22323.1\|2653389_2653992_+\|nucleoside-5-triphosphatase-RdgB	unknown	unknown	gnl\|CDD\|234648
LT992492.1\|SPM22339.1\|2671837_2672311_-\|putative-inner-membrane-protein	unknown	unknown	gnl\|CDD\|182143
LT992492.1\|SPM22334.1\|2668085_2669078_+\|4-hydroxythreonine-4-phosphate-dehydrogenase	unknown	unknown	gnl\|CDD\|234696
LT992492.1\|SPM22324.1\|2653985_2655161_+\|HemN-family-oxidoreductase	unknown	unknown	gnl\|CDD\|235550
LT992492.1\|SPM22338.1\|2671327_2671825_-\|dihydrofolate-reductase	unknown	unknown	gnl\|CDD\|365932

Protein	Function_ID	Function_description	E-value
LT992492.1\|SPM22337.1\|2670369_2671179_+\|diadenosine-tetraphosphatase	gnl\|CDD\|234673	PRK00166, apaH, symmetrical bis(5'-nucleosyl)-tetraphosphatase.	1.41419e-169
LT992492.1\|SPM22325.1\|2655381_2656302_-\|glutaminase	gnl\|CDD\|234880	PRK00971, PRK00971, glutaminase; Provisional.	0
LT992492.1\|SPM22320.1\|2651995_2652553_+\|Integral-membrane-protein-YggT	gnl\|CDD\|223833	COG0762, COG0762, Predicted integral membrane protein [Function unknown].	1.96316e-12
LT992492.1\|SPM22322.1\|2652888_2653320_+\|Uncharacterised-protein	gnl\|CDD\|379616	pfam14467, DUF4426, Domain of unknown function (DUF4426). Members of this entry are found mostly in g-proteobacteria, especially in Vibrio. Strangely enough, there seems to be one eukaryotic homolog in Nematostella vectensis (NEMVEDRAFT_v1g226006), where the PA0388-like domain is fused with a domain homogous to the Methionine biosynthesis protein MetW (see below). In several Pseudomonas species, but also in Vibrio vulnificus and Azotobacter vinelandii PA0388 homologs are genomic neighbors of Nucleoside 5-triphosphatase RdgB (dHAPTP, dITP, XTP-specific) (EC 3.6.1.15) and Methionine biosynthesis protein MetW. On the other hand, in most Vibrio species it appears as a part of a conserved operon involved in possible response to stress.	6.80534e-67
LT992492.1\|SPM22329.1\|2658820_2659948_+\|membrane-bound-lytic-murein-transglycosylase-C	gnl\|CDD\|183271	PRK11671, mltC, membrane-bound lytic murein transglycosylase MltC.	9.42926e-157
LT992492.1\|SPM22333.1\|2666800_2668096_+\|survival-protein-SurA	gnl\|CDD\|236758	PRK10770, PRK10770, peptidyl-prolyl cis-trans isomerase SurA; Provisional.	9.94454e-157
LT992492.1\|SPM22330.1\|2660711_2662337_+\|methyl-accepting-chemotaxis-protein	gnl\|CDD\|223910	COG0840, Tar, Methyl-accepting chemotaxis protein [Cell motility and secretion / Signal transduction mechanisms].	2.35326e-58
LT992492.1\|SPM22328.1\|2658446_2658719_+\|oxidative-damage-protection-protein	gnl\|CDD\|180059	PRK05408, PRK05408, oxidative damage protection protein; Provisional.	5.47988e-59
LT992492.1\|SPM22321.1\|2652552_2652843_+\|UPF0235-protein	gnl\|CDD\|179931	PRK05090, PRK05090, hypothetical protein; Validated.	2.12816e-59
LT992492.1\|SPM22332.1\|2664396_2666748_+\|organic-solvent-tolerance-protein	gnl\|CDD\|235158	PRK03761, PRK03761, LPS assembly outer membrane complex protein LptD; Provisional.	0
LT992492.1\|SPM22331.1\|2663383_2664238_-\|Dna-J-like-membrane-chaperone-protein	gnl\|CDD\|236512	PRK09430, djlA, co-chaperone DjlA.	1.60415e-153
LT992492.1\|SPM22327.1\|2657371_2658433_+\|A/G-specific-adenine-glycosylase	gnl\|CDD\|182805	PRK10880, PRK10880, adenine DNA glycosylase.	0
LT992492.1\|SPM22326.1\|2656412_2657132_-\|tRNA-(guanine-N(7)-)-methyltransferase	gnl\|CDD\|234649	PRK00121, trmB, tRNA (guanine-N(7)-)-methyltransferase; Reviewed.	6.01203e-111
LT992492.1\|SPM22335.1\|2669091_2669907_+\|dimethyladenosine-transferase	gnl\|CDD\|234708	PRK00274, ksgA, 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA.	3.27746e-146
LT992492.1\|SPM22336.1\|2669978_2670359_+\|CO2+/MG2+-efflux-protein-ApaG	gnl\|CDD\|180098	PRK05461, apaG, CO2+/MG2+ efflux protein ApaG; Reviewed.	1.16069e-74
LT992492.1\|SPM22323.1\|2653389_2653992_+\|nucleoside-5-triphosphatase-RdgB	gnl\|CDD\|234648	PRK00120, PRK00120, dITP/XTP pyrophosphatase; Reviewed.	4.099e-127
LT992492.1\|SPM22339.1\|2671837_2672311_-\|putative-inner-membrane-protein	gnl\|CDD\|182143	PRK09917, PRK09917, threonine/serine exporter.	7.45224e-67
LT992492.1\|SPM22334.1\|2668085_2669078_+\|4-hydroxythreonine-4-phosphate-dehydrogenase	gnl\|CDD\|234696	PRK00232, pdxA, 4-hydroxythreonine-4-phosphate dehydrogenase; Reviewed.	0
LT992492.1\|SPM22324.1\|2653985_2655161_+\|HemN-family-oxidoreductase	gnl\|CDD\|235550	PRK05660, PRK05660, radical SAM family heme chaperone HemW.	0
LT992492.1\|SPM22338.1\|2671327_2671825_-\|dihydrofolate-reductase	gnl\|CDD\|365932	pfam00186, DHFR_1, Dihydrofolate reductase.	1.40644e-92

>LT992492.1|SPM22329.1|2658820_2659948_+|membrane-bound-lytic-murein-transglycosylase-C
MRKLVLCITALLLSGCSREFIEKIYDVDYEPTNRFAKNLAELPGQFQKDTAALDALINSFSGNIEKRWGRRELKIAGKNNYVKYIDNYLSRSEVNFTEGRIIVETVSPIDPKAHLRNAIITTLLTPDDPAHVDLFSSKDIELKGQPFLYQQVLDQDGQPIQWSWRANRYADYLIANHLKVKQVDFKKAYYVEIPMVKDQIDIRGYKYASIVRKASRKYDIPEDLIYAIIKTESSFNPYAVSWANAYGLMQVVPKTAGRDVFKLVKNRSGEPSPEYLFNPENNIDTGTAYFYILKNRYLKEVRHPTSLEYSMISAYNGGTGGVLSTFSSDRQRAMRDLNALQPNQVYWALTKKHPNAEARRYLEKVTKFKKEFNAG
>LT992492.1|SPM22328.1|2658446_2658719_+|oxidative-damage-protection-protein
MARTVFCTRLQKEADGLDFQLYPGELGKRIFDNICKEAWAQWQTKQTMLINEKKLNMMDPEHRKLLEQEMVNFLFEGKEVHIEGYTPPAK
>LT992492.1|SPM22327.1|2657371_2658433_+|A/G-specific-adenine-glycosylase
MTPFAQAILTWYDAYGRKNLPWQQNKNAYRVWLSEIMLQQTQVATVIPYFERFLERFPTVHALAAAPQDEVLHFWTGLGYYARARNLHKAAQMVVSEYGGEFPTDLEQMNALPGVGRSTAAAVLSSVYKKPHAILDGNVKRTLARCFAVEGWPGQKSVENQLWHYAEMHTPKVDVDKYNQAMMDMGAMICTRSKPKCSLCPVESFCLAKQQGNPQEYPGKKPKTDKPVKATWFVMLYHDNAVWLEQRPQSGIWGGLYCFPQSEIANIQTTIDQRAIGDSTITSQKTLIAFRHTFSHYHLDITPILLQLSRKPDIVMEGSKGLWYNLSHPDEIGLAAPVKQLLHTLPFDIDSHI
>LT992492.1|SPM22326.1|2656412_2657132_-|tRNA-(guanine-N(7)-)-methyltransferase
MSEVITQEYTEDGKVLRRIRSFVRREGRLTKGQEAAMKECWPTMGIDYQPELLDWQQVFGNDNPVVLEIGFGMGASLVEMAKNAPEKNFLGIEVHSPGVGACLASAREAGVTNLRVMCHDAVEVFAHMIPDNSLHTLQLFFPDPWHKKRHHKRRIVQLEFAEMVRQKLMIGSGVFHMATDWENYAEHMVEVMNQAPGFANLATDGDYVPRPDERPLTKFEQRGHRLGHGVWDIKYQRTA
>LT992492.1|SPM22325.1|2655381_2656302_-|glutaminase
MKPTADILASIIEEVRPLTSKGIVADYIPALAKVPSDKLGIAVFTNQGEVITAGDAQEGFSIQSISKVLSLTLAMGLYQPDELWSRVGKEPSGQAFNSLIQLEMEQGIPRNPFINAGAIVVCDMLQSRLSAPRQRLLEFVRQLSGEPQIAYDKVVAASEMMHSDRNAAIAYLMRSFGNFHNEVIPVLHNYFHACALKMSCVELAKTFSYLANKGVSVVTGETVITPTQSKQTNALLATCGLYDGAGEFAYRVGMPGKSGVGGGIIAVVPGEMTIAVWSPALDQSGNSLAGTRALELLAQRIGRSIF
>LT992492.1|SPM22324.1|2653985_2655161_+|HemN-family-oxidoreductase
MLTPPSLSLYIHIPWCVQKCPYCDFNSHAQKGEIPEQEYLDALLQDLDRDIERYQLKGDPRLLHSIFIGGGTPSLISAEGIARLLQGVAERIAFKPEIEITMEANPGTIEAQRFAGYRTAGVTRISIGVQSFEPEKLARLGRIHGQQEAVRAAQLAHQIGLNSFNLDLMHGLPDQTPEQALADLDQAIALNPPHLSWYQLTIEPNTLFYSKPPKLPDEDALWDIFELGHQKLSDAGYVQYEISGYSKPGYQCQHNLNYWRFGDYLGIGCGAHGKLSFADGRIVRTTKTKHPRGYLAALNNLAKAYLDSEQLVADQDKPFEFFMNRFRLIEPCPKADFTATTGLTIDVIRPTLDWALSEGYLSEDDQHWQITEKGKLFLNDLLEAFMADEEE
>LT992492.1|SPM22323.1|2653389_2653992_+|nucleoside-5-triphosphatase-RdgB
MKKIVLATGNQGKVREMADLLSDFGFDVVAQSEFNVPEAAETGTTFIENAIIKARHAAQITGLPTIADDSGLEVDYLNGAPGIYSARYAGEHASDGDNLNKLLMAMQDVPDDQRSARFHCVLVLMRHADDPTPIVCHGKWEGKILTAPHGSNGFGYDPIFWVPEENCASAELEPVRKKQLSHRGKALQKLFKAIEEQRTC
>LT992492.1|SPM22322.1|2652888_2653320_+|Uncharacterised-protein
MRKWMMSLLLTLLALPASAEQQKTIKDIEVHYSAFNSTFLTPKVASSYQLTRNGYTAILNISVLDRASLGKPATEAKLTGHAKNLIGNLRELSFKQVKEGNAIYYLAEVPISNEEMLTFDIDVDAGLKGAGKLTFSQKFYTEQ
>LT992492.1|SPM22321.1|2652552_2652843_+|UPF0235-protein
MAAAWREGDDLLLRLYIQPKASRDSIVGLHGEELKVAITAPPIDGKANAHLSKYLAKLCKVAKGSVVVEKGELGRHKQVRILQPSQIPAEIAALIE
>LT992492.1|SPM22320.1|2651995_2652553_+|Integral-membrane-protein-YggT
MNSLSFLINTLFDLYIMVVILRIWLQAARADFYNPFSQFIVKATQPVVGPLRRVIPSIGSIDLATIVFAYVLCVLKFMALVLIASSGSVSFSADFLFLGLLSLIKAAGGLLFWVLLIRAILSWVSQGRSPIEYVFHQLTEPMLAPIRRIIPVMGGFDLSVLVLFIVLQFANFLMGDVIGPIWYQL
>LT992492.1|SPM22330.1|2660711_2662337_+|methyl-accepting-chemotaxis-protein
MKLKTQAYLLSAIILAALLALTATGLWTLRVASNLDNKARVTELFNSAYSILTEVEKLAQEGKMSEPEAKALATRLMRNNLYKDNEYVYVADENMTFVATPLDPQLHDTSFHDFKDGKGNSVGRLIQDVLRHQSGKLVEYTWTQKQADGSIEEKLSIARKTPHWGWVVGTGIGFNEVNARFWSTAQWQLSLCVVIAVAILSLLLVAIRKILLIIGGEPNEVRSAVQAVAQGRIRREFVIKAPKESIYGAVQQMSSSLADLVAKLEQSMVALRSELAGAGTRAKSIAELTDSQQQSTAMIATAMTEMASSANQVADSARDTAFNTDQADQQSQHTQKLIHNTVSNIQGLATQLQTASTAVADLDLDVKNIAKVLDVIGDIAEQTNLLALNAAIEAARAGEQGRGFAVVADEVRNLAGRTQTSTKEIQQMIHNLQEGSRNAIQTIQICGQTSQSSVQESENAASALALIVSALESVSSMSHQIATAAAEQTQVSDDIARRINMIEESGSKLSRVVMESHNSTQTLTKLARELEQWAAHFEVTR
>LT992492.1|SPM22331.1|2663383_2664238_-|Dna-J-like-membrane-chaperone-protein
MHIFGKILGAFFGFLFGGPFGAIFGIFLGHQFDKARRLNQAGFQSGTFGAGPSQAERQEEFFKSAFSVMGHVAKAKGQVTKEEIQLATIMMDRMNLTLEQKRAAQDAFRDGKESDFPLEQVLERVKIATGGRFDLLQFFLELQVSSAFADGDVHPSERQVLHRIARGLGFSSEQLERRLRMQEAAFRFQQGGGFGGSQQQSHSGQQWQQPSSRHQLADAYEVLGVSESASAQEVKRAYRKLMNEHHPDKLMAKGLPPEMMNVAKEKSQQIQHAYELIRKEKGIK
>LT992492.1|SPM22332.1|2664396_2666748_+|organic-solvent-tolerance-protein
MSCFSRTFLAASISAALFAPQIQAEASVDDNRAQLPNGEQCLVNQPEPTNPGQQPINVEADKLEAINGQKATYSGNVVVVQGKKRIAADNVTLHQQENVVVAEGNVQFSDGEIKTHSTKATNHLNTDEMTLENTRYQFLCEPGRGEAVYVSKTGKAVYEIEDGSITSCPDGDNAWRMRASSIDVDQNEEIATFYNPRLEVQNVPVFYLPYLTVPIGDTRKTGFLYPTASYGSRNGYSFEVPIYWNLAPQYDLETTFNYMQKRGTQLNSVFRYLTDFGAGQIKSEYLADDQLHTELGDRWAFQYEHNGIFQQAWKFEIDYSKVSDINYFSDLDSGVGNREDGQLIQEGRATYRSDNWDSALLVRDFQLLTKDTTSTNLPYRLMPQLSYNYYAPETMKYLDLDLVSHVSRFETDARGKPSATRVHIEPGLKIPFSNTWGNWTTEARVLGTYYQQDLDKTTDAKLEESVTRVIPEIRSVAGIVLERDTVLLDDYTQTLEPKIQYLYVPEKYQDNIGLYDSTLLQTDYYGLFRSRKYSGVDRIESANQVSYGASTRFFDSNYKERLNIAFGQIFYLDSKLNPSNKNPDSTSDKTSYSAWAVEMDFNFADYLFYHGGIQYDIDSQAVQLGNSTLEYRVASGYIQANYRYVAKDYIRNTVGDSITNIDDITRDGISQAGILAGYQLSRKWSASGQYYYDLTTDEALEWLANLTYTSDCWYVGFTYSNQLKSWNGNFVTDPYATPIYENNFSFNIGIIGFGTSIGAGSSMTGVDSAGNSLGYGRPFFLNN
>LT992492.1|SPM22333.1|2666800_2668096_+|survival-protein-SurA
MKLWKPTLISVLSALTLFNAHAEPKQLDSVAVIVNSGVILQSDVDSALKTIKANAKQNKQPLPQETVLREQVLEKLIIDTLQQQEADRIGVKIDDNRLNEAIKEIAKNNQQTQEQLIASVAQEGLTYPEFREQVRKEMAASDARNALVRRRINILPAEVDTLAELLAQETDATVQYKISHIQLRVDDGQDKSTAETLANKLVNDLRNGADFAQMAYAYSKGPKALQGGDWGWMRKEEMPTIFADQIKMQNKGSIIGPFRSGVGFHILKIDDVKGLETVAVTEVNARHILIKPTIILSDEGAQKQLNEFVQRIKNGEVTFAELAQQYSQDPGSAAQKGELGYQTPDLYVPEFKHQIETLPVGQISEPFKTVHGWHIVEVLDRREVDRTDSALKNKAYRILFNRKFNEEASAWLQELRASAFVEVLKDEKDEQ
>LT992492.1|SPM22334.1|2668085_2669078_+|4-hydroxythreonine-4-phosphate-dehydrogenase
MSSKRIIVTAGEPAGIGPDLVLALSAQDWPHQLVVCADKALLAQRATQLGIQVKLLDYQRDNPVQAQQAGTLLVEHIPLAEPVVAGQLNPANGHYVLKTLERAAKGCMNGEFDAIVTGPVHKGVINRAGVAFSGHTEFFAEQSKTPLVVMMLATEGLRTALVTTHLPLAEVPQAITCERLEQIVHILHKDLVEKFAIAEPKIYVCGLNPHAGEDGVLGMDEIETITPTLQRLREQYGMQLVGPLPADTIFSEKYLQQADAVLGMYHDQVLPVLKYKGFGRSVNITLGLPFIRTSVDHGTALDLAGTGQADAGSFWTALAYAIELVDKKAQ
>LT992492.1|SPM22335.1|2669091_2669907_+|dimethyladenosine-transferase
MRNDVHLGHKARKRFGQNFLNDPYIIDGIVSAINPKPGQNLVEIGPGLGAITEPVGREVDKFTVIELDRDLAERLRNHPELASKLTIHEGDAMRFDFKQLVKPNNKLRVFGNLPYNISTPLMFHLFEFHRDIQDMHFMLQKEVVNRLAAGPGTKAYGRLTVMAQYYCKVVPVLEVPPSAFVPPPKVDSAVVRLVPYEDLPHPATSLEWLDRVVREGFNQRRKTVRNCYKGLAEPETLETLGINPGMRPENLTLAQFVALANWLDATHKTHA
>LT992492.1|SPM22336.1|2669978_2670359_+|CO2+/MG2+-efflux-protein-ApaG
MDVSLPCIKIQVQTRYIEEQSNPEYQRFVFAYLITIKNLSSQTVQLMSRRWLITDADGKQTVVEGDGVVGEQPRIKANDEYTYSSGTALDTPVGVMQGQYLMIDEQGESFTVEIEPFRLAVPHVLN
>LT992492.1|SPM22337.1|2670369_2671179_+|diadenosine-tetraphosphatase
MANYIVGDIQGCFDELQQLLKQAEFNSQLDTLWFAGDLVARGPKSLETLRFVYQLGDAARVVLGNHDLHLLSVALGHHSAKRRDQTQAVLDAPDAAPLLDWLRQQPLLAEHQEFVLCHAGISPQWDLATARQAAQEVESVLRSPEWSTLIEQMYSDQPDAWHPTLQGIDRLRYIVNAFTRMRFCFPDGRLDMQCKLPPKEVTDGSLLPWFQLPQRIALEKTVIFGHWAALEGYVSETVIGLDTGCVWGGTLTMLRWEDKHYFSQAALPA
>LT992492.1|SPM22338.1|2671327_2671825_-|dihydrofolate-reductase
MMISMIAAMADQRIIGKDNQMPWHLPADFAWFKRCTLGKPVVMGRKTYQSIGRPLPGRHNIVISRDASLQIEGVDVVTSIEAALAKAGEVDEVMIIGGGSLYAACLPMAHKLYITEIHAKLDGDTQFPEWGSDWLERSREHYPADEKNAYGMDFVIFERQYPLPT
>LT992492.1|SPM22339.1|2671837_2672311_-|putative-inner-membrane-protein
MLSWDLFFGLLNDMFFAAIPAVGFALVFNVPVPALKYCALGGALGHGSRYLMMHFGVPIEWASFFAATLVGMVGVYWSRRFLAHPKVFTVAALIPMVPGVFAFKAMIALVEINHVGFSPELMEALLENFLKAMFIIAGLAVGLAVPGLLFYRRKPII

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Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity
LT992492_1	1.1\|2660228\|37\|LT992492\|PILER-CR	2660228-2660264	37	NC_049942	Escherichia phage JLK-2012, complete sequence	23524-23560	4	0.892
LT992492_1	1.1\|2660228\|37\|LT992492\|PILER-CR	2660228-2660264	37	NC_021742	Serratia liquefaciens ATCC 27592 plasmid unnamed, complete sequence	35428-35464	4	0.892

1. spacer 1.1|2660228|37|LT992492|PILER-CR matches to NC_049942 (Escherichia phage JLK-2012, complete sequence) position: , mismatch: 4, identity: 0.892

taggtcgccagttcgattccggcagccggcaccactt	CRISPR spacer
caggtcgccagttcgattccggtagccggcaccatat	Protospacer
.*********************.***********. *

2. spacer 1.1|2660228|37|LT992492|PILER-CR matches to NC_021742 (Serratia liquefaciens ATCC 27592 plasmid unnamed, complete sequence) position: , mismatch: 4, identity: 0.892

taggtcgccagttcgattccggcagccggcaccactt	CRISPR spacer
taggtcaccagttcgattccggtagccggcaccaatc	Protospacer
******.***************.*********** *.

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

647262 : 653879

Staphylococcus_phage(66.67%)

The bacterium proteins that are colored denote the protein is present at specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin' and 'tRNA')

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
SPM20561.1\|647262_648396_+	gamma-glutamyl kinase	A0A1X9I5D0	Streptococcus_phage	1.4e-64	40.4
SPM20562.1\|648407_649658_+	gamma-glutamyl phosphate reductase	A0A1X9I5D4	Streptococcus_phage	5.0e-100	49.5
SPM20563.1\|649757_650207_+	transcriptional regulator NrdR	NA	NA	NA	NA
SPM20564.1\|650232_651336_+	riboflavin biosynthesis protein RibD	A0A2H4PQS8	Staphylococcus_phage	1.3e-43	33.1
SPM20565.1\|651340_651994_+	riboflavin synthase subunit alpha	A0A2H4PQS5	Staphylococcus_phage	1.2e-31	38.2
SPM20566.1\|652034_653144_+	3,4-dihydroxy-2-butanone 4-phosphate synthase/GTP cyclohydrolase II	A0A2H4PQS2	Staphylococcus_phage	6.7e-64	39.8
SPM20567.1\|653408_653879_+	6,7-dimethyl-8-ribityllumazine synthase	A0A2H4PQS3	Staphylococcus_phage	6.8e-34	48.7

DBSCAN-SWA_2

1133964 : 1143229

Vibrio_phage(62.5%)

integrase,transposase

attL 1131513:1131526|attR 1148710:1148723

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
1131513:1131526	attL	NA	NA	NA	NA
SPM21007.1\|1133964_1134231_+	phage protein	M1Q554	Vibrio_phage	1.4e-07	33.8
SPM21008.1\|1134632_1135007_+	middle operon regulator-related protein	NA	NA	NA	NA
SPM21009.1\|1134993_1135314_+	transcriptional regulator	NA	NA	NA	NA
SPM21010.1\|1135332_1135911_+	Uncharacterised protein	NA	NA	NA	NA
SPM21011.1\|1135971_1136343_+	Uncharacterised protein	NA	NA	NA	NA
SPM21012.1\|1136391_1136751_+	Mu-like prophage FluMu protein gp41	A0A2I7S9E0	Vibrio_phage	3.7e-40	67.8
SPM21013.1\|1136885_1137926_+	Mu-like prophage FluMu protein gp42	A0A2I7S9D9	Vibrio_phage	2.6e-126	72.5
SPM21014.1\|1137968_1138277_+\|transposase	ISVch4 transposase, OrfA	Q716C1	Shigella_phage	7.1e-32	76.3
SPM21015.1\|1138519_1139146_+\|transposase	transposase OrfAB subunit B	Q716C2	Shigella_phage	1.1e-92	72.9
SPM21016.1\|1139359_1141312_+	putative helicase	Q2NP48	Hyphantria_cunea_nuclear_polyhedrosis_virus	3.1e-19	26.7
SPM21017.1\|1141442_1141898_-\|integrase	phage integrase	A0A1V0E8G8	Vibrio_phage	7.8e-43	58.4
SPM21018.1\|1141993_1143229_-\|integrase	Phage integrase	A0A1V0E8G8	Vibrio_phage	1.2e-146	62.2
1148710:1148723	attR	NA	NA	NA	NA

DBSCAN-SWA_3

1465163 : 1507171

Vibrio_phage(47.06%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
SPM21293.1\|1465163_1465331_+	Uncharacterised protein	E3U9J0	Vibrio_phage	2.3e-13	100.0
SPM21294.1\|1465323_1465491_+	Uncharacterised protein	NA	NA	NA	NA
SPM21295.1\|1466529_1467081_-	tlcR protein	NA	NA	NA	NA
SPM21296.1\|1467257_1468826_+	plasmid replication protein Cri	A7BJY2	Enterobacteria_phage	2.7e-58	32.3
SPM21297.1\|1468830_1469115_+	Uncharacterised protein	NA	NA	NA	NA
SPM21298.1\|1469244_1469937_+	Uncharacterised protein	NA	NA	NA	NA
SPM21299.1\|1470014_1470182_+	Uncharacterised protein	E3U9J0	Vibrio_phage	2.3e-13	100.0
SPM21300.1\|1470174_1470759_+	Phage protein	E3U9I9	Vibrio_phage	6.4e-114	100.0
SPM21301.1\|1471248_1471587_-	transcriptional repressor RstR	U5TNA1	Satellite_phage	7.3e-54	100.0
SPM21302.1\|1471712_1472792_+	RstA1 protein	U5TQR3	Satellite_phage	2.2e-213	100.0
SPM21303.1\|1472772_1473144_+	RstB2 protein	U5TMH5	Satellite_phage	5.7e-68	99.2
SPM21304.1\|1473237_1473462_+	RstC protein	U5TMI6	Satellite_phage	1.1e-34	100.0
SPM21305.1\|1473973_1474312_-	transcriptional repressor RstR	U5TNA1	Satellite_phage	7.3e-54	100.0
SPM21306.1\|1474437_1475517_+	RstA1 protein	U5TQR3	Satellite_phage	2.2e-213	100.0
SPM21307.1\|1475497_1475878_+	RstB2 protein	A0A0N9HE73	Vibrio_phage	1.4e-69	100.0
SPM21308.1\|1475877_1476021_+	Uncharacterised protein	NA	NA	NA	NA
SPM21309.1\|1476013_1476262_+	colonization factor	A0A0N7F140	Vibrio_phage	8.8e-33	100.0
SPM21310.1\|1476368_1477556_+	orfU	A0A142I701	Vibrio_phage	1.4e-200	100.0
SPM21311.1\|1477561_1477846_+	accessory cholera enterotoxin	A0A0F6YNQ7	Vibrio_phage	4.0e-45	100.0
SPM21312.1\|1477842_1479042_+	zona occludens toxin	A0A142I6Z1	Vibrio_phage	5.3e-240	100.0
SPM21313.1\|1479133_1479910_+	cholera enterotoxin, A subunit	A0A142I6Y0	Vibrio_phage	1.3e-151	100.0
SPM21314.1\|1479906_1480281_+	cholera enterotoxin subunit B	D1GID8	Vibrio_virus	5.9e-65	100.0
SPM21315.1\|1481194_1481374_-	Uncharacterised protein	Q9MBU8	Vibrio_virus	6.4e-25	100.0
SPM21316.1\|1481504_1482584_+	RstA1 protein	U5TQR3	Satellite_phage	2.2e-213	100.0
SPM21317.1\|1482564_1482942_+	RstB2 protein	A0A0N9HJ67	Vibrio_phage	6.8e-69	99.2
SPM21318.1\|1482941_1483085_+	Uncharacterised protein	NA	NA	NA	NA
SPM21319.1\|1483077_1483326_+	colonization factor	A0A0N7F140	Vibrio_phage	7.5e-32	98.8
SPM21320.1\|1483432_1484620_+	orfU	Q8LTP3	Vibrio_virus	2.4e-192	99.7
SPM21321.1\|1484625_1484910_+	accessory cholera enterotoxin	A0A0F6YNQ7	Vibrio_phage	4.0e-45	100.0
SPM21322.1\|1484906_1486106_+	zona occludens toxin	A0A023W604	Vibrio_virus	1.4e-237	99.2
SPM21323.1\|1486197_1486974_+	cholera enterotoxin, A subunit	A0A142I6Y0	Vibrio_phage	1.3e-151	100.0
SPM21324.1\|1486970_1487345_+	cholera enterotoxin subunit B	D1GID0	Vibrio_virus	1.3e-64	100.0
SPM21325.1\|1488258_1488438_-	Uncharacterised protein	Q9MBU8	Vibrio_virus	6.4e-25	100.0
SPM21326.1\|1488568_1489648_+	RstA1 protein	U5TQR3	Satellite_phage	2.2e-213	100.0
SPM21327.1\|1489628_1490006_+	RstB2 protein	A0A0N9HJ67	Vibrio_phage	6.8e-69	99.2
SPM21328.1\|1490005_1490149_+	Uncharacterised protein	NA	NA	NA	NA
SPM21329.1\|1490141_1490390_+	colonization factor	A0A0N7F140	Vibrio_phage	7.5e-32	98.8
SPM21330.1\|1490496_1491684_+	orfU	Q8LTP3	Vibrio_virus	5.3e-192	99.5
SPM21331.1\|1491689_1491974_+	accessory cholera enterotoxin	A0A0F6YNQ7	Vibrio_phage	4.0e-45	100.0
SPM21332.1\|1491970_1493170_+	zona occludens toxin	Q93AU9	Vibrio_virus	1.1e-237	99.5
SPM21333.1\|1493533_1507171_-	RTX toxin RtxA	B3Y8K3	Vibrio_virus	8.4e-07	100.0

DBSCAN-SWA_4

2248935 : 2256128

Anguillid_herpesvirus(16.67%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
SPM21973.1\|2248935_2249364_-	nucleoside diphosphate kinase	D2E8E1	Anguillid_herpesvirus	1.0e-20	38.4
SPM21974.1\|2249567_2250857_-	aminopeptidase B	Q6GYZ8	Mycoplasma_phage	1.7e-34	34.3
SPM21975.1\|2251076_2251271_-	Fe-S cluster assembly protein	NA	NA	NA	NA
SPM21976.1\|2251319_2251658_-	ferredoxin	NA	NA	NA	NA
SPM21977.1\|2251671_2253522_-	chaperone protein HscA	A0A0N9QXN8	Chrysochromulina_ericina_virus	5.3e-106	41.1
SPM21978.1\|2253545_2254061_-	co-chaperone HscB	NA	NA	NA	NA
SPM21979.1\|2254109_2254433_-	iron-sulfur cluster assembly protein	A0A2H4N7N5	Lake_Baikal_phage	3.0e-25	50.5
SPM21980.1\|2254493_2254877_-	NifU-related protein	A0A218MKD1	uncultured_virus	5.4e-53	78.2
SPM21981.1\|2254913_2256128_-	cysteine desulfurase	A0A0H3TPH2	Faustovirus	1.1e-32	30.5

DBSCAN-SWA_5

2572054 : 2579278

uncultured_Mediterranean_phage(33.33%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
SPM22245.1\|2572054_2572942_+	cysteine synthase B	A0A1X9I5K7	Streptococcus_phage	4.1e-56	41.1
SPM22246.1\|2573212_2575798_+	DNA mismatch repair protein MutS	A0A1V0SC35	Catovirus	2.2e-33	23.0
SPM22247.1\|2575890_2576898_-	RNA polymerase sigma factor RpoS	F4YCU2	Synechococcus_phage	6.0e-35	34.2
SPM22248.1\|2576971_2577907_-	lipoprotein NlpD	D7RWE0	Brochothrix_phage	7.8e-05	34.7
SPM22249.1\|2577906_2578533_-	protein-L-isoaspartate O-methyltransferase	A0A1B1IU40	uncultured_Mediterranean_phage	2.0e-36	52.1
SPM22250.1\|2578525_2579278_-	stationary phase survival protein SurE	A0A1B1ITZ2	uncultured_Mediterranean_phage	8.0e-69	53.3

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

2. LT992493

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

193588 : 240153

Shigella_phage(23.08%)

transposase,tRNA,integrase,tail

attL 185743:185758|attR 214944:214959

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
185743:185758	attL	NA	NA	NA	NA
SPM25633.1\|193588_194740_+\|integrase	integrase	A0A1P8DTG6	Proteus_phage	3.1e-56	33.0
SPM25634.1\|194785_195766_+\|transposase	ISVch5 transposase	Q38213	Escherichia_phage	3.0e-156	82.2
SPM25635.1\|196059_197247_+	Uncharacterised protein	NA	NA	NA	NA
SPM25636.1\|197767_198298_+	uropathogenic specific protein	A0A2R3ZY74	Campylobacter_phage	3.9e-09	40.7
SPM25637.1\|198297_198969_+	Uncharacterised protein	NA	NA	NA	NA
SPM25638.1\|198970_199321_+	Uncharacterised protein	NA	NA	NA	NA
SPM25639.1\|199589_201518_+\|tRNA	threonyl-tRNA synthetase	A0A2K9L297	Tupanvirus	2.0e-127	37.6
SPM25640.1\|201683_202073_+	translation initiation factor IF-3	A0A2L0UZ54	Agrobacterium_phage	2.6e-10	34.6
SPM25641.1\|202176_202371_+	50S ribosomal protein L35	NA	NA	NA	NA
SPM25642.1\|202412_202766_+	50S ribosomal protein L20	NA	NA	NA	NA
SPM25643.1\|202847_203810_-	site-specific recombinase IntI4	A0A1P8DJJ6	Virus_Rctr41k	1.0e-63	44.7
SPM25644.1\|204149_204350_+	vco30	NA	NA	NA	NA
SPM25645.1\|204485_204812_+	Var1-like protein	NA	NA	NA	NA
SPM25646.1\|206046_206298_+	fumarylacetoacetate hydrolase	NA	NA	NA	NA
SPM25647.1\|206450_206882_+	Uncharacterised protein	NA	NA	NA	NA
SPM25648.1\|207032_207662_+	acetyltransferase	M1HKK6	Paramecium_bursaria_Chlorella_virus	5.8e-20	37.8
SPM25649.1\|207817_208570_+	short chain dehydrogenase/reductase family oxidoreductase	F2NZ40	Diadromus_pulchellus_ascovirus	3.7e-21	31.6
SPM25650.1\|209231_209636_+	Uncharacterised protein	NA	NA	NA	NA
SPM25651.1\|210844_211522_+	HNH endonuclease	NA	NA	NA	NA
SPM25652.1\|211683_212892_+	deoxyguanosinetriphosphate triphosphohydrolase-likeprotein	NA	NA	NA	NA
SPM25653.1\|213053_213758_+	HNH endonuclease	NA	NA	NA	NA
SPM25654.1\|213915_214254_+	Uncharacterised protein	NA	NA	NA	NA
SPM25655.1\|214407_214725_-	Plasmid stabilization system protein	NA	NA	NA	NA
SPM25656.1\|214743_214986_-	prevent-host-death protein	NA	NA	NA	NA
214944:214959	attR	NA	NA	NA	NA
SPM25657.1\|215229_215616_+	NUDIX hydrolase	NA	NA	NA	NA
SPM25658.1\|215794_216016_+	Predicted Fe-S protein	NA	NA	NA	NA
SPM25659.1\|216273_216810_+	N-acetyltransferase GCN5	NA	NA	NA	NA
SPM25660.1\|216946_217462_+	outer membrane lipoprotein Blc	A0A1W6JNX6	Morganella_phage	2.2e-46	48.8
SPM25661.1\|217611_218109_-	acetyltransferase	NA	NA	NA	NA
SPM25662.1\|218105_218378_-	Uncharacterized protein conserved in bacteria	NA	NA	NA	NA
SPM25663.1\|219145_219592_+	Uncharacterised protein	NA	NA	NA	NA
SPM25664.1\|219739_220009_-	plasmid stabilization system protein	NA	NA	NA	NA
SPM25665.1\|220001_220280_-	DNA-damage-inducible protein J	NA	NA	NA	NA
SPM25666.1\|221592_221979_+	glyoxalase family protein	NA	NA	NA	NA
SPM25667.1\|222173_222425_+	fumarylacetoacetate hydrolase	NA	NA	NA	NA
SPM25668.1\|222638_222881_+	Uncharacterised protein	NA	NA	NA	NA
SPM25669.1\|224232_224700_+	general stress protein 17O	NA	NA	NA	NA
SPM25670.1\|224827_225490_+	Uncharacterised protein	NA	NA	NA	NA
SPM25671.1\|225927_226173_+	helix-turn-helix protein CopG family	NA	NA	NA	NA
SPM25672.1\|226268_227063_+	Uncharacterised protein	NA	NA	NA	NA
SPM25673.1\|227736_228210_+	Uncharacterised protein family (UPF0157)	NA	NA	NA	NA
SPM25674.1\|228358_228985_+	Lysine exporter protein	NA	NA	NA	NA
SPM25675.1\|229125_229806_+	Uncharacterised protein	NA	NA	NA	NA
SPM25676.1\|229985_230147_+	Uncharacterised protein	NA	NA	NA	NA
SPM25677.1\|230896_231292_+	lipoprotein	NA	NA	NA	NA
SPM25678.1\|231419_231719_-	Plasmid stabilization element ParE, putative	NA	NA	NA	NA
SPM25679.1\|231715_231958_-	transcriptional regulator	NA	NA	NA	NA
SPM25680.1\|232186_232765_+	Uncharacterised protein	NA	NA	NA	NA
SPM25681.1\|232938_233100_+	Uncharacterised protein	NA	NA	NA	NA
SPM25682.1\|233302_233986_+	Uncharacterised protein	NA	NA	NA	NA
SPM25683.1\|234199_234466_+\|transposase	transposase IS3	NA	NA	NA	NA
SPM25684.1\|234620_235220_+	Uncharacterised protein	NA	NA	NA	NA
SPM25685.1\|235500_236436_+	Restriction endonuclease	NA	NA	NA	NA
SPM25686.1\|236653_237676_+	Uncharacterised protein	NA	NA	NA	NA
SPM25687.1\|237620_238223_+	Uncharacterised protein	S5FXQ0	Shigella_phage	6.5e-05	28.5
SPM25688.1\|238283_238850_-\|tail	phage tail tape measure family protein	A0A2I7S9D9	Vibrio_phage	1.6e-05	61.0
SPM25689.1\|238975_239602_-\|transposase	transposase OrfAB subunit B	Q716C2	Shigella_phage	1.1e-92	72.9
SPM25690.1\|239844_240153_-\|transposase	ISVch4 transposase, OrfA	Q716C1	Shigella_phage	7.1e-32	76.3

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

Overview of predicted results

Overview of the results

Cas Category Instructions

Results visualization

1. LT992492

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Click the colored protein region to show detailed information

Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection

2. LT992493

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection