CRISPRimmunity

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Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Self targeting spacer number	Target MGE spacer number	Prophage number	Anti-CRISPR protein number
LR134487	Kocuria rosea strain NCTC7512 genome assembly, chromosome: 1	1 crisprs	DEDDh,WYL,DinG,csa3,cas3	2	3	1	0

Results visualization

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CRISPR-Cas detection and classification

Crispr_ID: LR134487_4

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

LR134487_4

3488036-3488120

Orphan

Consensus_repeat	Method
CGACCGGAAGCGACCCCTCGCGGC	CRISPRCasFinder

1 spacers

The CRISPR arrays of LR134487_4

>merge|LR134487|4|3488036-3488120|CRISPRCasFinder
CGACCGGAAGCGACCCCTCGCGGCCGGAGGAGCATAGAAAGCGACTCCTCGCGGCGGAGGAGCGCCGGAAGCGACCCCTCGCGGC

>LR134487|4|4|3488036-3488120|CRISPRCasFinder
CGACCGGAAGCGACCCCTCGCGGC	CGGAGGAGCATAGAAAGCGACTCCTCGCGGCGGAGGA
GCGCCGGAAGCGACCCCTCGCGGC

Protein	Signature genes	Signature genes Name	Protein_function
LR134487.1\|VEI52105.1\|3475288_3476815_-\|Xylulose-kinase	unknown	unknown	gnl\|CDD\|212659
LR134487.1\|VEI52104.1\|3474766_3475060_-\|Uncharacterised-protein	unknown	unknown	unknown
LR134487.1\|VEI52119.1\|3493319_3493997_+\|uncharacterized-Actinobacterial-protein	unknown	unknown	gnl\|CDD\|371689
LR134487.1\|VEI52110.1\|3481657_3483082_-\|Methionine-gamma-lyase	unknown	unknown	gnl\|CDD\|225428
LR134487.1\|VEI52124.1\|3497943_3498213_-\|Levodione-reductase	unknown	unknown	gnl\|CDD\|235506
LR134487.1\|VEI52122.1\|3496394_3497525_-\|Uncharacterised-protein	unknown	unknown	gnl\|CDD\|376263
LR134487.1\|VEI52117.1\|3491129_3492173_-\|Putative-aliphatic-sulfonates-binding-protein-precursor	unknown	unknown	gnl\|CDD\|270274
LR134487.1\|VEI52123.1\|3497685_3497904_+\|Uncharacterised-protein	unknown	unknown	unknown
LR134487.1\|VEI52107.1\|3479022_3480507_+\|Na+/alanine-symporter	unknown	unknown	gnl\|CDD\|376500
LR134487.1\|VEI52115.1\|3488125_3490252_-\|Acetyl-coenzyme-A-synthetase	unknown	unknown	gnl\|CDD\|234677
LR134487.1\|VEI52120.1\|3494082_3494547_+\|Uncharacterised-protein	unknown	unknown	gnl\|CDD\|379152
LR134487.1\|VEI52106.1\|3477032_3478865_+\|Dihydroxy-acid-dehydratase	unknown	unknown	gnl\|CDD\|237104
LR134487.1\|VEI52121.1\|3494882_3496301_+\|Pristinamycin-IIA-synthase-subunit-A	unknown	unknown	gnl\|CDD\|274819
LR134487.1\|VEI52109.1\|3481166_3481661_-\|acetyl-coenzyme-A-synthetase-(ADP-forming),-alpha-domain	unknown	unknown	gnl\|CDD\|224745
LR134487.1\|VEI52112.1\|3484552_3486172_-\|Na(+)/dicarboxylate-symporter	unknown	unknown	gnl\|CDD\|273267
LR134487.1\|VEI52113.1\|3486251_3486974_-\|Succinate-dehydrogenase-iron-sulfur-subunit	unknown	unknown	gnl\|CDD\|181038
LR134487.1\|VEI52118.1\|3492268_3493102_-\|Aliphatic-sulfonates-import-ATP-binding-protein-SsuB	unknown	unknown	gnl\|CDD\|224041
LR134487.1\|VEI52108.1\|3480578_3481100_+\|OsmC-like-protein	unknown	unknown	gnl\|CDD\|376823
LR134487.1\|VEI52116.1\|3490311_3491133_-\|Putative-aliphatic-sulfonates-transport-permease-protein-ssuC	unknown	unknown	gnl\|CDD\|223673
LR134487.1\|VEI52111.1\|3483415_3484483_+\|D-malate-dehydrogenase-[decarboxylating]	unknown	unknown	gnl\|CDD\|181281

Protein	Function_ID	Function_description	E-value
LR134487.1\|VEI52105.1\|3475288_3476815_-\|Xylulose-kinase	gnl\|CDD\|212659	cd07770, FGGY_GntK, Gluconate kinases; a subfamily of the FGGY family of carbohydrate kinases. This subfamily is composed of a group of gluconate kinases (GntK, also known as gluconokinase; EC 2.7.1.12) encoded by the gntK gene, which catalyzes the ATP-dependent phosphorylation of D-gluconate and produce 6-phospho-D-gluconate and ADP. The presence of Mg2+ might be required for catalytic activity. The prototypical member of this subfamily is GntK from Lactobacillus acidophilus. Unlike Escherichia coli GntK, which belongs to the superfamily of P-loop containing nucleoside triphosphate hydrolases, members in this subfamily are homologous to glycerol kinase, xylulose kinase, and rhamnulokinase from Escherichia coli. They have been classified as members of the FGGY family of carbohydrate kinases, which contain two large domains separated by a deep cleft that forms the active site. This model spans both the N-terminal domain, which adopts a ribonuclease H-like fold, and the structurally related C-terminal domain. Some uncharacterized homologous sequences are also included in this subfamily. The Lactobacillus gnt operon contains a single gntK gene. The gnt operons of some bacteria, such as Corynebacterium glutamicum, have two gntK genes. For example, the C. glutamicum gnt operon has both a gluconate kinase gntV gene (also known as gntK) and a second hypothetical gntK gene (also known as gntK2). Both gluconate kinases encoded by these genes belong to this family, however the protein encoded by C. glutamicum gntV is not included in this model as it is truncated in the C-terminal domain.	5.59832e-131
LR134487.1\|VEI52119.1\|3493319_3493997_+\|uncharacterized-Actinobacterial-protein	gnl\|CDD\|371689	pfam11716, MDMPI_N, Mycothiol maleylpyruvate isomerase N-terminal domain.	0.000109867
LR134487.1\|VEI52110.1\|3481657_3483082_-\|Methionine-gamma-lyase	gnl\|CDD\|225428	COG2873, MET17, O-acetylhomoserine sulfhydrylase [Amino acid transport and metabolism].	0
LR134487.1\|VEI52124.1\|3497943_3498213_-\|Levodione-reductase	gnl\|CDD\|235506	PRK05565, fabG, 3-ketoacyl-(acyl-carrier-protein) reductase; Provisional.	1.61983e-16
LR134487.1\|VEI52122.1\|3496394_3497525_-\|Uncharacterised-protein	gnl\|CDD\|376263	pfam18844, baeRF_family2, Bacterial archaeo-eukaryotic release factor family 2. Bacterial family of the archaeo-eukaryotic release factor superfamily. Likely to play roles in biological conflicts or regulation under stress conditions at the ribosome. This family contains a well-conserved 'FP' motif in the catalytic loop.	2.24427e-43
LR134487.1\|VEI52117.1\|3491129_3492173_-\|Putative-aliphatic-sulfonates-binding-protein-precursor	gnl\|CDD\|270274	cd13556, PBP2_SsuA_like_1, Substrate binding domain of putative sulfonate binding protein, a member of the type 2 periplasmic binding fold superfamily. This subfamily includes the periplasmic component of putative ABC-type sulfonate transport system similar to SsuA. These domains are found in eubacterial SsuA proteins that serve as initial receptors in the ABC transport of bicarbonate, nitrate, taurine, or a wide range of aliphatic sulfonates, while other closest homologs are involved in thiamine (vitamin B1) biosynthetic pathway and desulfurization (DszB). After binding the ligand, SsuA interacts with a cognate membrane transport complex comprised of two integral membrane domains and two cytoplasmically located ATPase domains. This interaction triggers the ligand translocation across the cytoplasmic membrane energized by ATP hydrolysis. The SsuA proteins belong to the PBP2 superfamily of periplasmic binding proteins that differ in size and ligand specificity, but have similar tertiary structures consisting of two globular subdomains connected by a flexible hinge. They have been shown to bind their ligand in the cleft between these domains in a manner resembling a Venus flytrap.	4.01104e-126
LR134487.1\|VEI52107.1\|3479022_3480507_+\|Na+/alanine-symporter	gnl\|CDD\|376500	pfam01235, Na_Ala_symp, Sodium:alanine symporter family.	0
LR134487.1\|VEI52115.1\|3488125_3490252_-\|Acetyl-coenzyme-A-synthetase	gnl\|CDD\|234677	PRK00174, PRK00174, acetyl-CoA synthetase; Provisional.	0
LR134487.1\|VEI52120.1\|3494082_3494547_+\|Uncharacterised-protein	gnl\|CDD\|379152	pfam13380, CoA_binding_2, CoA binding domain. This domain has a Rossmann fold and is found in a number of proteins including succinyl CoA synthetases, malate and ATP-citrate ligases.	1.67197e-40
LR134487.1\|VEI52106.1\|3477032_3478865_+\|Dihydroxy-acid-dehydratase	gnl\|CDD\|237104	PRK12448, PRK12448, dihydroxy-acid dehydratase; Provisional.	0
LR134487.1\|VEI52121.1\|3494882_3496301_+\|Pristinamycin-IIA-synthase-subunit-A	gnl\|CDD\|274819	TIGR03860, FMN_nitrolo, FMN-dependent oxidoreductase, nitrilotriacetate monooxygenase family. This model represents a distinctive clade, in which all characterized members are FMN-binding, within the larger family of luciferase-like monooxygenases (LLM), among which there are both FMN- and F420-binding enzymes. A well-characterized member is nitrilotriacetate monooxygenase from Aminobacter aminovorans (Chelatobacter heintzii), where nitrilotriacetate is a chelating agent used in detergents. [Unknown function, Enzymes of unknown specificity].	0
LR134487.1\|VEI52109.1\|3481166_3481661_-\|acetyl-coenzyme-A-synthetase-(ADP-forming),-alpha-domain	gnl\|CDD\|224745	COG1832, COG1832, Predicted CoA-binding protein [General function prediction only].	1.62814e-51
LR134487.1\|VEI52112.1\|3484552_3486172_-\|Na(+)/dicarboxylate-symporter	gnl\|CDD\|273267	TIGR00785, Uncharacterized_transporter_HI_0020, anion transporter. The Divalent Anion:Na+ Symporter (DASS) Family (TC 2.A.47) Functionally characterized proteins of the DASS family transport (1) organic di- and tricarboxylates of the Krebs Cycle as well as dicarboxylate amino acid, (2) inorganic sulfate and (3) phosphate. The animal NaDC-1 cotransport 3 Na+ with each dicarboxylate. Protonated tricarboxylates are also cotransported with 3Na+. [Transport and binding proteins, Anions, Transport and binding proteins, Cations and iron carrying compounds].	2.28435e-73
LR134487.1\|VEI52113.1\|3486251_3486974_-\|Succinate-dehydrogenase-iron-sulfur-subunit	gnl\|CDD\|181038	PRK07570, PRK07570, succinate dehydrogenase/fumarate reductase iron-sulfur subunit; Validated.	7.939e-104
LR134487.1\|VEI52118.1\|3492268_3493102_-\|Aliphatic-sulfonates-import-ATP-binding-protein-SsuB	gnl\|CDD\|224041	COG1116, TauB, ABC-type nitrate/sulfonate/bicarbonate transport system, ATPase component [Inorganic ion transport and metabolism].	6.93049e-105
LR134487.1\|VEI52108.1\|3480578_3481100_+\|OsmC-like-protein	gnl\|CDD\|376823	pfam02566, OsmC, OsmC-like protein. Osmotically inducible protein C (OsmC) is a stress -induced protein found in E. Coli. This family also contains a organic hydroperoxide detoxification protein that has a novel pattern of oxidative stress regulation.	5.568e-16
LR134487.1\|VEI52116.1\|3490311_3491133_-\|Putative-aliphatic-sulfonates-transport-permease-protein-ssuC	gnl\|CDD\|223673	COG0600, TauC, ABC-type nitrate/sulfonate/bicarbonate transport system, permease component [Inorganic ion transport and metabolism].	3.47925e-64
LR134487.1\|VEI52111.1\|3483415_3484483_+\|D-malate-dehydrogenase-[decarboxylating]	gnl\|CDD\|181281	PRK08194, PRK08194, tartrate dehydrogenase; Provisional.	5.85637e-171

>LR134487.1|VEI52113.1|3486251_3486974_-|Succinate-dehydrogenase-iron-sulfur-subunit
MSTFSLTVDVWRQPSPEEPGDFTAYDVPDASPDMTVLELLDRINSQLEAAGEDPVVFDDGCREGVCGKCGVTVDGVAHGPERNAASCMQHLGAYRDGDRVRIEPLRAASFPVKRDLSVDKKALRLVAKAGKFPVAMKALANGGCISCGACVASCPNGSGQLFVGTMLRELPPLAKDDAERRERSARVIDASEEQFGPCSLLGDCVEVCPAGLPIENLSAVAQENLRNRFPRRSRDAPDPG
>LR134487.1|VEI52112.1|3484552_3486172_-|Na(+)/dicarboxylate-symporter
MSERPGKIVPEQGEFDYEPDDREPARPAAKTWLFRGLGLLLAFLVWFLLGGADVSADARVVGAVATLMAVWWMTEAVPLSITSLLPIVLFPVLTALTVAETTAPYANPIVFLFLGGFLIAIAMQKWNLHRRIALLTLRRVGTHPRQIILGLMISTAFLSMWVSNTATTLMMLPIGLSVLTLVVENSRNAGTRAETEAMGEDIRGGKAISDVIDDRDVRLFGVALVLAIAWAATIGGLGTLLGSPPNAIVAGYISDELDRTVGFAQWMMLGVPIVVVFIGLAWLLITRVIFRFNLAEVPGGKELINEEIAALGPMSKGEKTVLAVFVIAAFFWIVPGLLSGIGTIAEQLPWLDLFDDTVVAIGAGVLLFLLPGDKQGNMTLEWKDAEEGLPWGVLLLFGGGLSLAAAVAGTGLDAWFGAQVSGLGALPIVLLLAAVVTIVLLLTEITSNTATAATFIPILGGVAVGIGVDPMTLLIPAALAATCAFMLPVGTPPNAIVFATGQVKIAEMVRGGIVLNIVGVILITIFTVLIGPFALGLVI
>LR134487.1|VEI52111.1|3483415_3484483_+|D-malate-dehydrogenase-[decarboxylating]
MTRTRHYTIDVIAGDGIGQEVMPSALRCVDALAQAHGFTVDWRERGWGSDHYRAHSRMMPVDGIEQLASGDGVLLGAVGAPDIPDDVTLWGLLIPIRREFHQYVNLRPIRILPGVTSPLRGLDELDLVVVRENIEGEYSEVGGRLYRGRPEEMAVQESVFTRAGVTRIARYAAELAQRRTGRLVSATKSNGIIHTMPFWDEVVEATVAEYPGVTVEKVLIDALAARMVLKPRSVDVIVASNLFGDILSDLAAGAAGSIGVAPSANLNPEKTHPSMFEPVHGSAPDIAGQGIANPVGTMWAAAMMLDHLGETGAARHLESAFEQTMAGGTLTGDLGGTASTEQFTQAVLARCTDAP
>LR134487.1|VEI52110.1|3481657_3483082_-|Methionine-gamma-lyase
MADHQFGFRTRALHAGGTPDAEHGARAVPIYQTTSFVFKDADDAANLFALQKYGNIYSRIGNPTVAAFEERVASLEGGIGAVATASGMSAEFLVFAALAQAGDHIVASAQLYGGTVTQLDVTLRRFGIETTFVPGTDPADYRAAVRPGTKAVYTEVVANPSGDVADLEGLAAVAHEAGVPLVVDSTMTPPYLVRPIEHGADIVIHSATKFLGGHGTTLGGVVVESGRFDWANGNFPSMTEPVPTYNDVTWWGNFGEYGFLTRLRTEQLRDIGPALAPQSAWNLLQGVETLPQRMDAHLANARAVARWLEEDPRVSYVNWAGLPSHPHHERARRYLPLGPGSVFAFGVSPTERLGGREVGARFIENLQLASHLANIGDARTLVLHPASTTHQQLSAEQLVGAGVREDLIRLSVGLEDVEDILWDLDQALTAATGLPRETPGRAAGAPAGATTADDTADAAAAADAADVTTEGALR
>LR134487.1|VEI52109.1|3481166_3481661_-|acetyl-coenzyme-A-synthetase-(ADP-forming),-alpha-domain
MTAETRTWTGPSAPERLSILHGTESIAVVGASDKPSRASYFVSTYLLSSSPYRVYFVNPALREIMGRPVYPSLADLPETPDVVDAFRRHDDLPGVAHEAVAVGARTLWLQLGSWHEEAARIAEEGGLSVVMDRCLKIEHARFHGGLHLAGFNTGVISSKRQVLS
>LR134487.1|VEI52108.1|3480578_3481100_+|OsmC-like-protein
MESRTDIDASALRQLQAPLKERYREHPEEARTPLHARGDFRDTGLTTTVDSWAGPVRAGLHAATGGDGSDACSGDMLLDALAACAGVTLRSVATAMGVPIRSAELRAEGMFDARGTLGLDRSVAVGVQDVVLTVRLDTDADDATLQRLAKSTERYCVVSQSLNTDVAFVVERV
>LR134487.1|VEI52107.1|3479022_3480507_+|Na+/alanine-symporter
MELVTDLVGTLGGWVWNVVFAILIGVGLYLTVRTGVVQLRLVPEMFRVLNDKPGLAEDGQKGISAFRAFTVSAASRVGTGNIAGVAIAISLGGPGAVFWMWVIAGIGAASAFTESLLGQLYKIKAPTGYVGGPAYYMERGLGARWMGLLFAVVITFTYGFVFNSVQSNSIVDALGGSLGVDIAAAEALWFRILVGAVLVGLTAAIIFGGIRRISAVSEIVVPIMAVLYLLLGLLVVFLNLGEVPAVLGQILEGAFGIREFVTGGLIGVIIQGMKRGLFSNEAGMGSAPNAGATASVSHPAKQGFLQALGVYFDTWLICSITAFIVLLSNPEFGGREGASLTQSALALQLGNWAVHFLTVAIFLFAFTSVIGNYYYGESNVRFLTGSPAAMTAYRVLVLAFVFGGAVLALDLVWSLADLFMAVMATINLVALVLLAGVAVRVLRNYEAQRKQGLEPIFHSGDVPGLSGLGAWDGTDVVTTRGYWDELAQKPTRTR
>LR134487.1|VEI52106.1|3477032_3478865_+|Dihydroxy-acid-dehydratase
MPHLRSRTSTHGRNMAGARALWRATGMGDDDFGKPIVAIANSYTQFVPGHVHLKDMGDLVASAIREAGGVAKEFNTIAVDDGIAMGHDGMLYSLPSRDIIADSVEYMVNAHRADALVCISNCDKITPGMLLAALRLNIPVVFVSGGPMESGEAVEGVVEHRLDLVDAMSMAVDESVTDEQLGQIERNACPTCGSCSGMFTANSMNCLTEALGLSLPGNGTTLATQVARKELFLEAGRRVVDLCCRYYEQDDESVLPRNIATKAAFENAMALDIAMGGSTNTILHILAAAQEGEVDFTLPDIDALSRRVPCLCKVAPNSTRFHIEHVHRAGGIPALLGELRRAGLLNEDVHAVHAPDLATWLDEWDVRSGRSSERARALFHAAPGGVRTTQAFSTANTFESLDLDGEDGCIRSVEHAYTRDGGLAILYGNIAEDGAVIKTAGIDEELFHFVGRAFVVESQDEAVFEILSKNVEPGDIVVITHEGPKGGPGMQEMLYPTSYLKGLGLGRECALITDGRFSGGTSGLSIGHISPEAAAGGAVGLIETGDEIEIDVARRVLRVNVDDATLAARRQARGPAPWYPTKPRERQVSKALRAYASMVTSADKGAVRVI
>LR134487.1|VEI52105.1|3475288_3476815_-|Xylulose-kinase
MSHHPTPPRPDDGAAFSVDLREALDPLVMALDVGSTGSRGGLHDATGTPVRGYRHKIEHEFTTAADGTSVIDPDQVTEELAAVLDLVLADPALAGRVAGVALDTFASSLVGVGTDGAAVTTCFTYADSRCAEQLAGLRGELDEPAALQRTGARLHTSYLAPRLRWLRETDPEAWERARRWMSLGEYVHLRLLGTARAGTATAAWTGMLDRRTGRWDEPLLLACGLDAEQFNPLADPHEPIPDVAASVAERWPVLEGAAWFAPVADGLSANLGTGGSDASTMVVSTATSGAARVLLHRIPERVPPGLWCYRVDGARCLLGGALNDVGRALAWLERTVALPEGTTLADVAAADVSGSTPVVLPFFTGERSTGWAGGARAVFAGVSASSTGADLARGVLEGVAVSYARVAEQLEDAAGGVERVVASGSASQDVPELLQLLADALGKPVARAEFKRATLRGTALLALESLAPDVERASPPCGPEHRPVPGRAEHYRAARARFEELYGAVVGS
>LR134487.1|VEI52104.1|3474766_3475060_-|Uncharacterised-protein
MEDSLGREQAVGLHQALERASIGHRRLWVRYLYLGGTADELEVSAYLHQCLDLPVRERDLLSWAANTLLDRRYHPRVPSSADFLRHGTGHGAPERTA
>LR134487.1|VEI52115.1|3488125_3490252_-|Acetyl-coenzyme-A-synthetase
MSFSWSDPSSPTPNPALVSEEARIAFWEAQASRLDWARPWTTAHRFEAPRRLGTDEQGGPTWSVPEIAWFEGGRLNVAHNCVDRHVEAGRGAHVALYFEGEPGDRLVYTYADLQREVSRAANALLALGVEQGDRVVIYLPVIPETVIITLACARIGAVHSLVFGGFSAEALRFRVEDTGAKVLVTTDGQHRRGAVVPVKAVADEACSGDNAVEHVVVVRRTSRPGRTDLPRGTRGTHSKEEDAVPWTRGRDVWWHELVAEQSDVHVPEAFDAETPLFIIYTSGTTGRPKGLVHTMGGYLVQTAYTHALLFDLLPDVEDEDGALRPDELATVNDPAKVDSTVHWCTADLAWVTAHTYEIYGPLVNGVSEVIYEGTPNTPHWGRHFEVVERYGVTAYYTAPTLIRSLMGAFPKGVPPLYNLNSIRLLGSVGESINPEAWRWLRAQLGRDEVPFVDTWWQSETGATVMSPRPHDPQFAPPGTFDDGAPRTALKPGCATRAVPGISTRVVDEAGETVPPGVQGFVVVDRTGPSMARTVWGDPQRYLSSYWQAYAEQGWFLAGDGARYDEEGDIYILGRVDDVINVSGHRLSTIEIESALVTHPWVVEAGAAPVRDELTGHAVLAYVVLTPEAARVPAEELTAALREHVAHEIGPIAKPRAVVAVPDLPKTRSGKITRRLLAQLWEGAPLGDRSSLQNEESLERIADVLAARR
>LR134487.1|VEI52116.1|3490311_3491133_-|Putative-aliphatic-sulfonates-transport-permease-protein-ssuC
MTQPLSPAEEAERFDDAPAAAPRRRPLLRTGLGLLLPAALLLLWHVLSTAGVFSAVQLPAPLAVLEAGIGLLAGGDLWTHVGISTQRVLIGFLIGAGLGLVAGSFIGLSRWGSTLVGPTIGAFRAVPSLAWVPLLLLWIGLGEDSKVTLVAIGAFFPVYTTVSAALQHVDRNLIEAARAFGLHGLRLLTTVQLPAVMPSVISGLRLALAQAWLFLVAAELLGASMGLGWLLTDSQNNGRTDRLLLAIVLLAVLGKATDALLGVFEKWAAKKWA
>LR134487.1|VEI52117.1|3491129_3492173_-|Putative-aliphatic-sulfonates-binding-protein-precursor
MPLPQRSVRTVPALAAVLALGLTGCMAGEGSGEPPAEAAGDGTVRVDWATYNPLSVVIKEKGWLETAFAEEGIEVEWVQSAGSNKANEALRAEAVDIGSTAGSAALLARSNGTPIRVIDLYSQPEWSALVTTAGSEVQEVADLEGRTVAATKGTDPYFFLLQALDEAGVDAGDVTIQNLQHADGRAALENGQVDAWSGLDPIMAAAEENGAELFYRNVDFNTYGFLNAHESFLEEQPEAAQTVVDVYAYARQWAQENTEETAQLLAEAAGIELSVAEKVLERTNLDIDHVPGDEQLAVLEKVGPIFVESDDVAGQEQIDEALGSIVESSYAENADASRAAELVGAGQ
>LR134487.1|VEI52118.1|3492268_3493102_-|Aliphatic-sulfonates-import-ATP-binding-protein-SsuB
MSTSLLGSAPATAPGTAKRAFSVAFEHVGKAFPSGRETHHVLQDVDVTARPGEVLAVLGPSGCGKSTLLRAAAGLDTASAGRVLIDGTPVSGIDPRCAVAFQEPRLLPWRSVRENVRLGLPRGLPRDEGDAVIDDLLRLVGLTAHAGHRPRAVSGGMAQRASLARALARNPGVLLLDEPFGALDALTRLQMQDLLLTIHAAQPTTVLLVTHDVDEALQLADRIVVLGRTGPTAPASISTILAVPGERPRDRASEVLAELRVQLLGHLGVDTHHPATR
>LR134487.1|VEI52119.1|3493319_3493997_+|uncharacterized-Actinobacterial-protein
MTSRRSPARADLVRDACAAFGAVLDGLGDDDSWAATGCPGWSVRDLTHHCAMDALRGLVALHTPTTARPDRDAATYWTDWGGDPAGAAEARRHTRVAASMVLDWEPVRALHRETTRALVHAAQHCGDGAVRTQGHVLRVEDLLSTLAVEATVHHLDLIQHLDGSPGPAVDGLAEVRRVLTDLWEREPLEHWSDEHFARVGTGRAELTAQERAELGAHADLLPLFS
>LR134487.1|VEI52120.1|3494082_3494547_+|Uncharacterised-protein
MSRNDPAVIDRLMTRPGRWAVVGLTGNTARPAHSVAKRIRDDLGMTIVPVNPKGEPVHGEPGHRTLAEVPGPIDVVDCFVNSRRVGAVVDQAIAVGAAAVWLQLGVVDEDAAARAERAGLDVVMDTCPLIELRSLLRRGWTLDPGRTPPLAPPG
>LR134487.1|VEI52121.1|3494882_3496301_+|Pristinamycin-IIA-synthase-subunit-A
MSKRIALNAFDMTCVGHQAPGLWKHPRSRADEYNTIEYWTDVAQVLEKGLFDALFLADVVGYYDVYQNSAAPVIRDATQIPVNDPFMQISAMAAVTKHLGFGVTSAITYEQPYPLARKFATLDHLTKGRMGFNVVTSYLKSAAENHGLTEQISHDERYEIAEEFMEVCYKLWEGSWEDGAVRRDRVNGVFADPALVHPIQHHGKYFDVPGIGLTEPSPQRTPLIFQAGASSRGRAFAGRHAEAVFVGGIRPDLTRVVTDKLRDEAEASGRNRDDLKIFAMLHVIVDETDAKAEQKRRDYERYADTDGALGLVGGWSGLDLGRYDENDVLEYIKTDSIQSFLTPFTTADPHKKWTIKEVAKHISMGGMGVPIVGSPQTVADELERWIDEGGLDGINLAYHVSPGSFEDFVEWVVPELQKRGRYRTGYEGTTLRENLYGKGQTKVLGTHPAAAFRGAYAGRPSAADTPARQLAL
>LR134487.1|VEI52122.1|3496394_3497525_-|Uncharacterised-protein
MTSRFHRYAELFKSKGPWCTVYTDISTGTVDSLHAIDVLPENICRELERQGASKADLAAVEAAVRPAEGVPDPVCRYLLVRGGTVEIDEVLPGPRAGSGVVQVEDVPDLTPLFRHRPDDFPYVVAEVGRDGGEICLQHASRVRAADEQQIEGETEHLKKFPGGGWSQGRWQRHTEDVWRRNTEQVAEQIDKVVAESGAQLVVLAGDLRARGLVEEQLSKASRGMLSVVDANTRAEGSKSADFDHQVEERVAEVVAHRQQALLERLAEQQGRADPTAVSGMAPVVSALQQAQAETLLIDDTHLDTEQQLIALDAEPWIALEESEAVGAGVLGRVSADAALVRAAVLTDGRPVFVPPGALPHGTDVAALLRWSAAPAS
>LR134487.1|VEI52123.1|3497685_3497904_+|Uncharacterised-protein
MSDVPEYDEADRLEQSIDADQQDLDDQDEEAPVHAPDRSEASSLEANPDDLVEQDQEVVLDEPQEVSTLEEE
>LR134487.1|VEI52124.1|3497943_3498213_-|Levodione-reductase
MAGLTKSTALTHAADGIRTNAVAPGATATGIEAPMVSDLFPGRMAGFMQNIPYTATAEQVAAVIAFLASDDASNVNGVIMPSDGGWSAV

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Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity
LR134487_2	2.1\|2083345\|21\|LR134487\|CRISPRCasFinder	2083345-2083365	21	LR134487.1	3665390-3665410	2	0.905
LR134487_2	2.2\|2083390\|21\|LR134487\|CRISPRCasFinder	2083390-2083410	21	LR134487.1	2109022-2109042	2	0.905

1. spacer 2.1|2083345|21|LR134487|CRISPRCasFinder matches to position: 3665390-3665410, mismatch: 2, identity: 0.905

ggtcgtggtgtccggggtcgt	CRISPR spacer
ggtcgtggggtccgggggcgt	Protospacer
******** ******** ***

2. spacer 2.2|2083390|21|LR134487|CRISPRCasFinder matches to position: 2109022-2109042, mismatch: 2, identity: 0.905

gccggtggcgtcccgctccac	CRISPR spacer
gccggtgtcgtcccgcaccac	Protospacer
******* ******** ****

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity
LR134487_2	2.3\|2083435\|21\|LR134487\|CRISPRCasFinder	2083435-2083455	21	NZ_CP024895	Amycolatopsis sp. AA4 plasmid unnamed1, complete sequence	87011-87031	0	1.0
LR134487_2	2.1\|2083345\|21\|LR134487\|CRISPRCasFinder	2083345-2083365	21	NZ_CP016082	Streptomyces sp. SAT1 plasmid unnamed2, complete sequence	187364-187384	1	0.952
LR134487_2	2.2\|2083390\|21\|LR134487\|CRISPRCasFinder	2083390-2083410	21	NC_017590	Thermus thermophilus JL-18 plasmid pTTJL1802, complete sequence	113165-113185	1	0.952
LR134487_2	2.2\|2083390\|21\|LR134487\|CRISPRCasFinder	2083390-2083410	21	NC_017273	Thermus thermophilus SG0.5JP17-16 plasmid pTHTHE1601, complete sequence	78601-78621	1	0.952
LR134487_2	2.2\|2083390\|21\|LR134487\|CRISPRCasFinder	2083390-2083410	21	NZ_LR027519	Thermus thermophilus isolate TTHNAR1 plasmid 3, complete sequence	6505-6525	1	0.952
LR134487_2	2.3\|2083435\|21\|LR134487\|CRISPRCasFinder	2083435-2083455	21	NZ_CP032322	Azospirillum brasilense strain MTCC4035 plasmid p1, complete sequence	972269-972289	1	0.952
LR134487_2	2.3\|2083435\|21\|LR134487\|CRISPRCasFinder	2083435-2083455	21	NZ_LR134455	Tsukamurella tyrosinosolvens strain NCTC13231 plasmid 13, complete sequence	259471-259491	1	0.952
LR134487_2	2.2\|2083390\|21\|LR134487\|CRISPRCasFinder	2083390-2083410	21	MH271318	Gordonia phage Trine, complete genome	2873-2893	2	0.905
LR134487_2	2.3\|2083435\|21\|LR134487\|CRISPRCasFinder	2083435-2083455	21	NZ_CP043441	Cupriavidus campinensis strain MJ1 plasmid unnamed1, complete sequence	2312544-2312564	2	0.905

1. spacer 2.3|2083435|21|LR134487|CRISPRCasFinder matches to NZ_CP024895 (Amycolatopsis sp. AA4 plasmid unnamed1, complete sequence) position: , mismatch: 0, identity: 1.0

gtcggcggtgtccgggccggt	CRISPR spacer
gtcggcggtgtccgggccggt	Protospacer
*********************

2. spacer 2.1|2083345|21|LR134487|CRISPRCasFinder matches to NZ_CP016082 (Streptomyces sp. SAT1 plasmid unnamed2, complete sequence) position: , mismatch: 1, identity: 0.952

ggtcgtggtgtccggggtcgt	CRISPR spacer
ggtcgtggtgtccggggccgt	Protospacer
*****************.***

3. spacer 2.2|2083390|21|LR134487|CRISPRCasFinder matches to NC_017590 (Thermus thermophilus JL-18 plasmid pTTJL1802, complete sequence) position: , mismatch: 1, identity: 0.952

gccggtggcgtcccgctccac	CRISPR spacer
cccggtggcgtcccgctccac	Protospacer
 ********************

4. spacer 2.2|2083390|21|LR134487|CRISPRCasFinder matches to NC_017273 (Thermus thermophilus SG0.5JP17-16 plasmid pTHTHE1601, complete sequence) position: , mismatch: 1, identity: 0.952

gccggtggcgtcccgctccac	CRISPR spacer
cccggtggcgtcccgctccac	Protospacer
 ********************

5. spacer 2.2|2083390|21|LR134487|CRISPRCasFinder matches to NZ_LR027519 (Thermus thermophilus isolate TTHNAR1 plasmid 3, complete sequence) position: , mismatch: 1, identity: 0.952

gccggtggcgtcccgctccac	CRISPR spacer
cccggtggcgtcccgctccac	Protospacer
 ********************

6. spacer 2.3|2083435|21|LR134487|CRISPRCasFinder matches to NZ_CP032322 (Azospirillum brasilense strain MTCC4035 plasmid p1, complete sequence) position: , mismatch: 1, identity: 0.952

gtcggcggtgtccgggccggt	CRISPR spacer
gtcggcggtctccgggccggt	Protospacer
********* ***********

7. spacer 2.3|2083435|21|LR134487|CRISPRCasFinder matches to NZ_LR134455 (Tsukamurella tyrosinosolvens strain NCTC13231 plasmid 13, complete sequence) position: , mismatch: 1, identity: 0.952

gtcggcggtgtccgggccggt	CRISPR spacer
gtcggcggtatccgggccggt	Protospacer
*********.***********

8. spacer 2.2|2083390|21|LR134487|CRISPRCasFinder matches to MH271318 (Gordonia phage Trine, complete genome) position: , mismatch: 2, identity: 0.905

gccggtggcgtcccgctccac	CRISPR spacer
gccggtggcgtcccgctcctg	Protospacer
*******************

9. spacer 2.3|2083435|21|LR134487|CRISPRCasFinder matches to NZ_CP043441 (Cupriavidus campinensis strain MJ1 plasmid unnamed1, complete sequence) position: , mismatch: 2, identity: 0.905

gtcggcggtgtccgggccggt	CRISPR spacer
cgcggcggtgtccgggccggt	Protospacer
  *******************

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

1802595 : 1809934

Bacillus_phage(33.33%)

The bacterium proteins that are colored denote the protein is present at specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin' and 'tRNA')

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
VEI50595.1\|1802595_1803318_+	Probable transcriptional regulatory protein TcrX	W8CYM9	Bacillus_phage	2.5e-35	38.3
VEI50596.1\|1803330_1804797_+	Probable sensor histidine kinase TcrY	W8CYF6	Bacillus_phage	6.2e-25	29.2
VEI50597.1\|1804806_1805829_-	Ribonuclease HI	J9Q745	Salmonella_phage	7.2e-20	42.7
VEI50598.1\|1805889_1806597_-	putative Mg(2+) transport ATPase	G3MA03	Bacillus_virus	1.1e-16	41.0
VEI50599.1\|1806747_1808379_-	chaperonin GroEL	A0A240F779	uncultured_virus	1.2e-146	53.6
VEI50600.1\|1808629_1809229_-	Uncharacterised protein	NA	NA	NA	NA
VEI50601.1\|1809356_1809575_-	Uncharacterised protein	NA	NA	NA	NA
VEI50602.1\|1809571_1809934_-	Protein of uncharacterised function (DUF3263)	A0A0A7RXU7	Mycobacterium_phage	9.0e-10	53.8

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage