CRISPRimmunity

Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Self targeting spacer number	Target MGE spacer number	Prophage number	Anti-CRISPR protein number
LR214941	Mycoplasma orale strain NCTC10112 genome assembly, plasmid: 2	0 crisprs	NA	0	0	0	0
LR214940	Mycoplasma orale strain NCTC10112 genome assembly, chromosome: 1	1 crisprs	NA	0	1	0	0
LR214944	Mycoplasma orale strain NCTC10112 genome assembly, plasmid: 5	0 crisprs	NA	0	0	0	0
LR214943	Mycoplasma orale strain NCTC10112 genome assembly, plasmid: 4	0 crisprs	NA	0	0	0	0
LR214942	Mycoplasma orale strain NCTC10112 genome assembly, plasmid: 3	0 crisprs	NA	0	0	0	0

1. LR214940

CRISPR-Cas detection and classification

Crispr_ID: LR214940_2

• CRISPR_ID: LR214940_2
• CRISPR_location: 674217-674319
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 1 spacers

Consensus repeat of LR214940_2

Consensus_repeat	Method
GAAGGGAATCGAACCCTCGTATT	CRISPRCasFinder

spacers of LR214940_2

>2.1|674240|57|LR214940|CRISPRCasFinder
AGTGAAGTCACTAATGTTTTAACCAATAAACTATCCTTATAAAAAAATGGTGCGGGC

CRISPR arrays and Neighbor proteins around LR214940_2

CRISPR arrays

The CRISPR arrays of LR214940_2

>merge|LR214940|2|674217-674319|CRISPRCasFinder
TAAAGGAATCGAACCTTTTTATTAGTGAAGTCACTAATGTTTTAACCAATAAACTATCCTTATAAAAAAATGGTGCGGGCGAAGGGAATCGAACCCTCGTATT

>LR214940|2|2|674217-674319|CRISPRCasFinder
TAAAGGAATCGAACCTTTTTATT	AGTGAAGTCACTAATGTTTTAACCAATAAACTATCCTTATAAAAAAATGGTGCGGGC
GAAGGGAATCGAACCCTCGTATT

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
LR214940.1|VEU55999.1|660085_660397_+|glutamyl-tRNA(gln)amidotransferase-subunit-C	unknown	unknown	unknown
LR214940.1|VEU56000.1|660386_661712_+|aspartyl/glutamyl-tRNA-amidotransferase-subunit-A	unknown	unknown	gnl|CDD|235945
LR214940.1|VEU56012.1|685042_686050_+|transposase	unknown	unknown	gnl|CDD|225382
LR214940.1|VEU56004.1|665431_666463_+|Uncharacterised-protein	unknown	unknown	gnl|CDD|181988
LR214940.1|VEU56017.1|688285_688480_+|Uncharacterised-protein	unknown	unknown	unknown
LR214940.1|VEU56007.1|672300_672732_-|Uncharacterised-protein	unknown	unknown	gnl|CDD|379233
LR214940.1|VEU56009.1|674666_675272_+|FMN-dependent-NADH-azoreductase	unknown	unknown	gnl|CDD|234946
LR214940.1|VEU56008.1|672874_674152_+|Uncharacterised-protein	unknown	unknown	unknown
LR214940.1|VEU56015.1|687621_687810_+|50S-ribosomal-protein-L35	unknown	unknown	gnl|CDD|234676
LR214940.1|VEU56016.1|687837_688212_+|50S-ribosomal-protein-L20	unknown	unknown	gnl|CDD|179959
LR214940.1|VEU56006.1|671398_672235_-|transposase,-IS861	unknown	unknown	gnl|CDD|222853
LR214940.1|VEU56001.1|661704_663117_+|glutamyl-tRNA(Gln)-and/or-aspartyl-tRNA(Asn)-amidotransferase,-B-subunit	unknown	unknown	gnl|CDD|235489
LR214940.1|VEU56002.1|663118_664273_+|Ribonuclease-BN-like-family	unknown	unknown	gnl|CDD|224214
LR214940.1|VEU56010.1|675434_683831_+|Uncharacterized-ABC-transporter-permease-MG468-homolog	unknown	unknown	gnl|CDD|376899
LR214940.1|VEU56005.1|666462_671367_+|Valine--tRNA-ligase	unknown	unknown	gnl|CDD|235582
LR214940.1|VEU56013.1|686192_686672_-|Uncharacterised-protein	unknown	unknown	gnl|CDD|371241
LR214940.1|VEU56011.1|683832_684867_+|ABC-transporter-ATP-binding-protein	unknown	unknown	gnl|CDD|213222
LR214940.1|VEU56014.1|686969_687641_+|translation-initiation-factor-IF-3	unknown	unknown	gnl|CDD|234582
LR214940.1|VEU56003.1|664287_665388_+|LD-carboxypeptidase	unknown	unknown	gnl|CDD|132883
LR214940.1|VEU56018.1|688486_689605_+|tRNA-specific-2-thiouridylase-mnmA	unknown	unknown	gnl|CDD|234664

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
LR214940.1|VEU56000.1|660386_661712_+|aspartyl/glutamyl-tRNA-amidotransferase-subunit-A	gnl|CDD|235945	PRK07139, PRK07139, amidase; Provisional.	0
LR214940.1|VEU56012.1|685042_686050_+|transposase	gnl|CDD|225382	COG2826, Tra8, Transposase and inactivated derivatives, IS30 family [DNA replication, recombination, and repair].	3.12207e-67
LR214940.1|VEU56004.1|665431_666463_+|Uncharacterised-protein	gnl|CDD|181988	PRK09609, PRK09609, hypothetical protein; Provisional.	3.72272e-94
LR214940.1|VEU56007.1|672300_672732_-|Uncharacterised-protein	gnl|CDD|379233	pfam13518, HTH_28, Helix-turn-helix domain. This helix-turn-helix domain is often found in transposases and is likely to be DNA-binding.	3.65592e-07
LR214940.1|VEU56009.1|674666_675272_+|FMN-dependent-NADH-azoreductase	gnl|CDD|234946	PRK01355, PRK01355, azoreductase; Reviewed.	2.15213e-86
LR214940.1|VEU56015.1|687621_687810_+|50S-ribosomal-protein-L35	gnl|CDD|234676	PRK00172, rpmI, 50S ribosomal protein L35; Reviewed.	1.68163e-17
LR214940.1|VEU56018.1|688486_689605_+|tRNA-specific-2-thiouridylase-mnmA	gnl|CDD|234664	PRK00143, mnmA, tRNA-specific 2-thiouridylase MnmA; Reviewed.	3.83406e-174
LR214940.1|VEU56016.1|687837_688212_+|50S-ribosomal-protein-L20	gnl|CDD|179959	PRK05185, rplT, 50S ribosomal protein L20; Provisional.	1.97808e-61
LR214940.1|VEU56006.1|671398_672235_-|transposase,-IS861	gnl|CDD|222853	PHA02517, PHA02517, putative transposase OrfB; Reviewed.	2.55465e-37
LR214940.1|VEU56001.1|661704_663117_+|glutamyl-tRNA(Gln)-and/or-aspartyl-tRNA(Asn)-amidotransferase,-B-subunit	gnl|CDD|235489	PRK05477, gatB, Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB.	0
LR214940.1|VEU56002.1|663118_664273_+|Ribonuclease-BN-like-family	gnl|CDD|224214	COG1295, Rbn, Ribonuclease BN family enzyme [Replication, recombination, and repair].	2.37698e-11
LR214940.1|VEU56010.1|675434_683831_+|Uncharacterized-ABC-transporter-permease-MG468-homolog	gnl|CDD|376899	pfam02687, FtsX, FtsX-like permease family. This is a family of predicted permeases and hypothetical transmembrane proteins. Buchnera aphidicola LolC has been shown to transport lipids targeted to the outer membrane across the inner membrane. Both LolC and Streptococcus cristatus TptD have been shown to require ATP. This region contains three transmembrane helices.	4.95004e-10
LR214940.1|VEU56005.1|666462_671367_+|Valine--tRNA-ligase	gnl|CDD|235582	PRK05729, valS, valyl-tRNA synthetase; Reviewed.	0
LR214940.1|VEU56013.1|686192_686672_-|Uncharacterised-protein	gnl|CDD|371241	pfam10779, XhlA, Haemolysin XhlA. XhlA is a cell-surface associated haemolysin that lyses the two most prevalent types of insect immune cells (granulocytes and plasmatocytes) as well as rabbit and horse erythrocytes. This family has had DUF1267, pfam06895, merged into it.	0.00370127
LR214940.1|VEU56011.1|683832_684867_+|ABC-transporter-ATP-binding-protein	gnl|CDD|213222	cd03255, ABC_MJ0796_LolCDE_FtsE, ATP-binding cassette domain of the transporters involved in export of lipoprotein and macrolide, and cell division protein. This family is comprised of MJ0796 ATP-binding cassette, macrolide-specific ABC-type efflux carrier (MacAB), and proteins involved in cell division (FtsE), and release of lipoproteins from the cytoplasmic membrane (LolCDE). They are clustered together phylogenetically. MacAB is an exporter that confers resistance to macrolides, while the LolCDE system is not a transporter at all. An FtsE null mutants showed filamentous growth and appeared viable on high salt medium only, indicating a role for FtsE in cell division and/or salt transport. The LolCDE complex catalyzes the release of lipoproteins from the cytoplasmic membrane prior to their targeting to the outer membrane.	2.11344e-101
LR214940.1|VEU56014.1|686969_687641_+|translation-initiation-factor-IF-3	gnl|CDD|234582	PRK00028, infC, translation initiation factor IF-3; Reviewed.	5.07974e-59
LR214940.1|VEU56003.1|664287_665388_+|LD-carboxypeptidase	gnl|CDD|132883	cd07062, Peptidase_S66_mccF_like, Microcin C7 self-immunity protein determines resistance to exogenous microcin C7. Microcin C7 self-immunity protein (mccF): MccF, a homolog of the LD-carboxypeptidase family, mediates resistance against exogenously added microcin C7 (MccC7), a ribosomally-encoded peptide antibiotic that contains a phosphoramidate linkage to adenosine monophosphate at its C-terminus. The plasmid-encoded mccF gene is transcribed in the opposite direction to the other five genes (mccA-E) and is required for the full expression of immunity but not for production. The catalytic triad residues (Ser, His, Glu) of LD-carboxypeptidase are also conserved in MccF, strongly suggesting that MccF shares the hydrolytic activity with LD-carboxypeptidases. Substrates of MccF have not been deduced, but could likely be microcin C7 precursors. The possible role of MccF is to defend producer cells against exogenous microcin from re-entering after having been exported. It is suggested that MccF is involved in microcin degradation or sequestration in the periplasm.	2.038e-83

Protein sequences

>LR214940.1|VEU56008.1|672874_674152_+|Uncharacterised-protein
MDIMVKLVDKWKAVSESSWIMWVQIAVFCLTLILAFVTGFTKGPVVLIFVGVFLIGGWLIALGISKPIAAAIAKKVDLNKYMGKYGGEDFTNVFIKTLSSFLLFLITSIFAFISLIFMRVFRKLIKKPLEKRKENNKSTIGLRLAGGLIAPIAALPLASFSANVVGIAAKPESGVSKALNGMQKFLTFKQMSALSQYSPGLISVATLAADYLKNGSNDDSIFGSINTYFQQFFNQDNYSFKGIPNNIAINAKGEPTGDIDVEFYFSLKKVDSAGNVEYNKIKYFTDSDPSTVQKIINNFTRTEESFKIFEMILKRIDTVIYKDGKPEIEKYIENIDNAFKPDVGGHQLNFKANFSNIKVFLEPWQKIVIANDEYRQKVKWLILNIAGIANVQLPQTQEQLNESDAKGKVRYIFESMFDQFITKQK
>LR214940.1|VEU56007.1|672300_672732_-|Uncharacterised-protein
MQFKFKKEKRSKWNRDIKGYLKLKLDQKIKIVELYLQDFSILEISKIMKNSYSACYSVIEKYKKFGYNSFAMEKKKGRKSKINLDAQKAANFKINIENKIEDKDLLIKQLKEENKILKLENAIAKKVSALVQLKDSLTKKNSK
>LR214940.1|VEU56006.1|671398_672235_-|transposase,-IS861
MKKSTFYEILKSQNKPDKDENLKKLIFDLFNYNKGLYGYRRITFALRNKGTMINHKKVQKLMKSLNLFGKTLRRKNKYSSFKGDAHKNIPNLLLDKENITEDFFRYKRIFSSDKYLKILGTDVTEFKLKNEEKAYFSPVVDFENREILGYSISKSPNLRMIGKMLENVEENGHSLNNVLLHSDQGWQYTHEEYINYLKEKQTIQSMSRKGNCLDNSPTECLFSVIKREFWFEEENRFKSFKEFKTALEKYISYYNNDRIVNKLKGLSPVQYRNKSKHN
>LR214940.1|VEU56005.1|666462_671367_+|Valine--tRNA-ligase
MAKTKIKSLDFKDIRIYEKQANKWSYKLRNFNMHLEQNEILGIISYNANLQEDIFKLVTNKWKSLKSYQGLASVKFDYEKNLSIKNKQYIKNLAYSFDTKLIDNDSDDSKASQISLYQYLENYFVESNLLKTSLDDFSSAWKETFDNYGFYLKKEFALNFNNLEKTLKYMLEDLLEMLETFKVESARTSLSAINKNLEELEEKFTLIINKYKDIEYELMQISYNTINRFQKGEILLNYNQYVACRDKYIDAHNKYYNFFNLRKEIKKFKAQRRKEIRNEFFIQSNDVTLLLKKIYYDIKIENKFCMYKLSKAQSNKEYAYYLENYLIDNKISNLIKILLKNAKWLNMEIISIILQKIYELKTEIFDEFSFINLNKSKRNIRKNITLLVNNSFVIKEDFYKDIIDEKKANYIEKISQYSSKKKISTDEFEINFKNRFSLEELNQIKNEYIKIKNEYLWNYNNLYNDSTSVYSREMAQISKVKKANKNIFKKISSLFIHYLYSINSVVIDNYNSFDYKLITDYHVNMMKINNLWKTLESQRNDFQGYRKIFVNGLYHDCPIIRKFIIRSLVYTIMKKSNISLEKYNFSLDSFSNETKLKVEIEKIKINKPSLIVIGHYISKLSELMQKKFLNEINKYIFENNIIGIYLLNDFQVAKDVTTNIIFINNKKPIEQGKTLKIISNPINPLVKGFVGSADERTVENITDYISYLDDYENINRYEIDTDHKVWCTWEELNKWATKENVANPKLAALIFNEVEHSESVEVNFDNPNANEKDTELFDFSNTLLNKESKGEEMKPQFDHKIVEEKRVDKWNEAKFFSTHDLNKKAFTVILPPPNVTGKLHIGHAFGDYIQDTVIRYKKLQGFDVLWIPGKDHAGIATQAKIEKRLAEKKIDKYKIGRDKFLEEAIKWKDEYSKNITNQWNKLGLALDYDAERFTLDNDANEAVIKVFIDLYNDGLIYRDVKPINWDPILKTALSNIEVINKDVEQKMYYIKYMLKNSSSFIVVATTRLETMFSDVAIAINPSDIRAKNLLGKLVINPLTKKEIPIIASELIDKDFGTGLMKVSAHAMDDFNIIKDNKLELIECIDENGILNENAMQFKGLDRFEARKQIEAYLAKNNLIEKIEKITSAVGFSERSDCPIEILVQPQWFVKMKELAEGLLAHFNQEDKVNFIPKRFEDVLRKWMENVHDWTISRQIWWGHRIPAWYKDNKVLVQKNCPGDGWVQDEDVLDTWFSSALSPFVFLGWPQSTAKLDRYFPTSLLVTGYDIIFFWVARMYFQSLYFLDKKPFDTVLIHGLVRDSQGRKMSKSLGNGIDPIDVIDKYGSDVLRNALIFNSTPGQDINFGDDKINSAKLFINKFWNIARLISQIKIKDEIDYDLHKIDKFDLWILNKFIQLRKSIDDSMSKYEFTVIFKNIQNFIVNDLSSWYLEFLKLKSNKKFIHVLFRELLLTLHPFMPFLTDYLFETIYHEELLNNISYNFNVDFVQSDLQEIDSLINIITALRQYRESKKISKSELLKFHLEEFSLSTLYATIVNKMTNFEQEKNFDYLIEIPNARIYVKLNKENIKNDINDLEAKIKQLQIDIDFNKKMLENPKFLEKASKKIIAEKTEKLNNFISQIEGYKEELTKKKNKLNEQ
>LR214940.1|VEU56004.1|665431_666463_+|Uncharacterised-protein
MFETKKKKSDIRVPGGIRHYLSWARSKVSLGYKKWSSKRIAFVSVLIAISVVFFLISVRIIPISALPSYKFSFIGLPVKITGFIFGPIIGMLTGILADLISFLLVPTYYHYLYTISISIAGFIPGLCGYYFFGLNEMLFSNKYKIFKFKEIVDFFKKQYSEATIKGNTEDVQYFSECIAYYEVKIILLENKAKPIAMINFAFFSALAVLTIQVFLIISIFIKLDPSYFENNRFIKNKLFYIFATSSGFVGMGVFLILFRIFIKKRYQLFIDIMAIVTFSAILEFLNVIILSWADAATLKIDFWVNLANHSLTGPIKIFFNVVIITTTYRIVAPLLKSKEGDKF
>LR214940.1|VEU56003.1|664287_665388_+|LD-carboxypeptidase
MKKEFIKPFFLQKGDNIAIVSLSSGLLGEEIFKWELDLLILQIKKLGFNPIFMPNSLKGIKFLDEHPECRASDLKEAFTNKNIKGIFCAIGGFDTIRLTKFLFEDNHFVNIVKENPKIFLGFSDSTINHFMFNKIGLITFYGINVLSGIVEFNKKILSYNKRSLRLFLNNKKQNFIIKSSKYWFEEYSDYSINSLGKKRVKHKEKYGYEILNNSNNLKIIEGQILGGCIDSMYEIFENPLYRQINEIYNIFPNAKEWEDKIILLETSEQKPNPELFNKMLLKLKENNVFIKPKCIIVGKPQNEVYYHEYKNILTNFFKDIPVLYNFNIGHAHPKSILPFNSKIKINFKSKKVILFRKECLNLNNLK
>LR214940.1|VEU56002.1|663118_664273_+|Ribonuclease-BN-like-family
MCRKNKWNKIKSGWQNNKKDPENENKPELANDQVILNQTHNNFLEKILKAIIYGILYIAISRYLKSSRTKGKEIVNSAHTKLKSNEFAFIPIGYAMYLFLSFIPISLILVSIIASISPEYDTVFRYAILGKIIPGIDTILPKDISSIWTNPGGAISFLILTLSIIFFTSKGYSKFIISIDALYEHKTKYRAWKGWAKGIVVSISLIAILTLMLLLFTILMTFLVKKAEFGNKHNLSDMSTLIANALTNNDKSFTPLKALELKTEFWIIYYFIVVLTLPLFTYLGFAAFYLYAPSFKLKFNQVHPGALITAIPTSFFILIFGTLASIIKYDKFGPVAAFMYIILLVTFIAYFTYMGIIVNSSFYKTFINILTIDKKSIFANSKRN
>LR214940.1|VEU56001.1|661704_663117_+|glutamyl-tRNA(Gln)-and/or-aspartyl-tRNA(Asn)-amidotransferase,-B-subunit
MNNWELVIGIEIHIELNTKTKMFSPILNNYDSAINSNVSNIDLAYPGTLPLVNKEAIIKGLKLAKALNMQIDSLVRFDRKNYFYFDLPKGYQITQQFNPIGKAGKIKAKVDGVWKDIEIERIHLEEDTAKSIYEGNDILLNYNRAGVPLIEIVSKPVMHSAKEAAAYVDAIRLMALYLDISDAKMNEGSLRVDVNVSTRKKGTNVLNTKVEIKNLNSIANVEKAINFEFLRQVKCYESNKEVSQETRKFDEQKLETVVMRKKGNAEDYKYFPEPNILPILISKEVIDSVKLNTLPFEKEKEYLAKGLNELQISQFINNLAYASFFEKIDITDFKKKANLFFSNIVPFLNDNKLDIVDLKISLPEVKELFNYLIEGKIDKKNVMTILDLKQKNSAKSLKEIIESQKMFIEVLNISLEEIVDEIFKENPNLLKDFNNNFDKNKKYLIGQIMKKTQGKAKLEKLDIILANKVK
>LR214940.1|VEU56000.1|660386_661712_+|aspartyl/glutamyl-tRNA-amidotransferase-subunit-A
MKIKGNLELAKKELLANKNNAVAYVFKDAKLNNSKGLLANCVFTLKDNFADKNVKARASSLFLETFKPSYKATCLELLEAAGATCVGRTNLDEFGLGGSGEHSAYGKIFHPKNNEYLVGGSSSGAAATFTNAISFAIGSDTGDSVRKPASNIGAVGFKPSYGAVSRYGMFAFASSLDTVSYFTHNVSDLILLSSVLYKQDRKHDLTSENLEFNYNDIKLVKPKTVAYLDCFNELEPSVKEAYQNIINKLQKENVKLIKVSEDKILLKSIDTIYRIIAFSEASSNLSNLTNITFGQKLNDSWVKNAFESRSKKIGKMAQARLILGSYFLEKDNQIKYFVQAKKIRNYLKEYFTNIHTQADIFIYPASYGSAPKIGEKQKSTYMDYILTYANLVGNPSMTIKLGKNKNNGLPFNLTIDAKIYNDTKLFSHALYIEELIGDQNE
>LR214940.1|VEU55999.1|660085_660397_+|glutamyl-tRNA(gln)amidotransferase-subunit-C
MDEKKVIELAKSIYIKPSKEVINLTLNLYKNVKDGLQDLEEKLSKKVENYKPLARVSDTIIDFNSLREDKEDDSFKLELPDILNNASSTKDDYIVTKKAQNED
>LR214940.1|VEU56009.1|674666_675272_+|FMN-dependent-NADH-azoreductase
MSNKVLVLLSSPLPKDKSISTYLTIKFVHDYLKQHPNDEINYLDLNEIESASTILKASNFSAFFENNFISDKYINLLKNSNKLIIAAPMINFNVPTTLKSFIDYICIANKTFSYKYSAKGESKGLLDNLDVQIIATQGAPKDWYLFSSHKDYLEGLWKFLGANVKPTIHVTSLKTKEFSQKSREEIYELYKSEIEKRAKEF
>LR214940.1|VEU56010.1|675434_683831_+|Uncharacterized-ABC-transporter-permease-MG468-homolog
MRRLFKEVFKSLAKNKITMICLTILIFITSGIFTLFFNIKTSYTSTVNSYNKVSRLHDLSVDLDVNPAGNIPNGGFDLIDKENKVIEKSPSEFNWDNEKSHVDYTLSLPKEHQEYIPLKDTKPENQFENWTFGPSFDNYYIRTKDFLDFYKNSKLNSNVKFDIDDYSSSNNKKLSDGITFDQARRFSLSGSSDYTFPLFKKIAGKEKFVNVTKEEQINNASNFVFNSELKLSNIAQFIYNTNKKSEYLINPVSLYLNVKENKASINIDDYNKWKDENALHVIKGSDLLNMLGFYEQEPNSGKFYFNKSKQDSTDTDIKLSSGYTSIENNINANDKIASNFKLINYLLTHQINNNALEYANITAGNYLLPSSWVRKRTITGKFLWYRYKLNWDEQLNNSNWKGSYLKYINNLKQTNSDKYDELRYFSIWTKKITTKYEHNGKINTNEEELSLHLDEDINKNDLNIKFLAADEWDGKALLGLKNNWVDGKKYKSILEIESVLNPSLNSNPVENAKKLISSAELKNNQTILKDGASQYARLAILKEIVKEVGEENIGIRQTLTIETIDELTKIKNVFHFINTGDKDQKIKGIKQNVGKLQEEAFNKTILHSSINNKDIDEFLLKSKDPSIIKKLPPVYTKEIINAIFDNYTPDFNYFVPDIRYVDYYDYYENTRFLKTVKNGKLLLLTDEADAKVSEGAKILGAITKVDTDKYIVLKNENNRWIKDNSWFLDKHYFTLDELYQQLVIKGLTIKAEIGKDGWAWVNPTFKNSLSLPVAFGSINTDLTNDIIQNKSVALLIEYAKKILYESDLMKIISKDALEVILYSAQKAIEDNGYHEMLSVGKSNPYTLTKVLFDLLNNLIISINNNSNSAYKYANINGEVFIKSTLNNIIDYFEKQYLDSGSTIEERDQKFVDILNKFLGLLDFNLENIFPSFLNIKLRDLFKFFKDKSQVFIFFKNLIDSINFSKFSSLIQDWYKKYPLQNLTDNKRAYWVLSSHQIVVDFLKSVDSNKFRTTMKNIINNVDFNKILNPNIENSLFDIWKKANQKANVAISADDEQNIKTFFINLSAYDITNPALAYKNVSEGLSEIIDNISVEKFANTLEGFLKNYRWAVEANAKVYDNFNTKTLSDVDYLSAFVSSMVSSNNSSVSNKVIAIQNALIKLANLSFKTQEISKTLKLNIPADEPGKISLTNLLALTKLSFPNNNSTSSTSHAFSPYDEEKIKDILGNINISIANKMPVELNKEEIAFLTNYVLIQEDEFNNLDTMKQKVEYYLEFIKKLKINNFKTNSEYKFDSAEASKDILSYADLGYFSSLINEESTIVDKPLLSKLHSVLAKYLGSMIGKGDGPIIDKTLNLYSMWIKLAYELNALGEKTVSYVKNPYTGENEKVINKKNDLTYDQITFILKGLLNLAKDSEVTNLFNSFEEVKEKLPGIGILGSQPGSYDALALQLSLAHTETNKLVSNIEKIFNTNAKFQNYFNSLKASGIELSDKNIQDFKSILLKHKYELVYNFGYIASANMMPTFYLSALEKFVSTFINNKDISADSKLKPLITNNFDFDLVYKMTLNASKTSQIFSIINLPSSLMNPLIALSFPQILLSYLLSNNPNEGNAGFIVRKLFSNLTNMNAEEIRSMLLPLFDKFLPDDLKIENKSDANIVIDLSWFNYLITNKLRPNNEDLEFYGLNLTKTFKDVLYKIIQPISIYNLISYTDAGSYLAKVNYGYLNKNNKEVYKGDLSKYLKSPLEMQRFVDSLDAKYKVRINTIEYVIIGVDTTADYLYPAINESNLQVDTKTQALLYVNQKGFDRIYSAYPTFALKEYVLVKAPSKLTKSGKEIYQIGKSPNDLKNIFNKIISSVSSSNSQKVYLNNEVDYLNPERSIRVTTTAKLISTIKSVTFYAVFILIILVAFIVYFIVKRYIEDRNKVIGILLAQGYTTKEIAISFCAFGWIPTLIGTIFGYITGLLLQSPTMKIFSIYWTLENKIISFSLPTLLFTIIVPFVLISLLIFLITWFSVRKKPTELMSGLVEVSIGNFSQKIMSIFRRSTIKFKYIISTAINNFWKFISLLLAFSTTSLITMLFMSTGNVFNKSINQTYENRNYRYKLDLETPTTEGGPFVTYNKNDLSKYLYVPNDLSGGGSSSTGSQLDYQNPNFFRPGYNFNTDVKVNKFDPVVLTKSSLDLLLDMSVSLSPWDITYANMPETQRARVTEIFKRVSKAMEQTQNLVPCHWSVAAPGYMSVKDINKYYADQKAGLPEDLSNRQSFFAFTTVGSGSSSDGLPKFKLVEWNPETGSYAKPVLVSTSKYRIQYRNFLTEAYKKINLKDFFVSFGGIYWNDSTNEKYTYADVGIGFYTKKSKEEKNKDYKIYGYQKDSKFIKIYSEDGKNLTQALENYNWDENKEIPLVVNHVTSKILGISEGSILNANIKNHVNRFLYRALGKDSPKVNYKFKIIGVSNTYINNEFITRKDILDKILGFDSLTRRLCEAKKDELDAIKNISDPKDYKKIEENFIKQYEAFNGILSNDETPVQTIDTLTTYSPLGYWGASATFDVDGSDNFVIWDFFKRIFLSDGSKFTSAYEHMVKSYNEYDKSNLDYKETLLRKLKITNDQFNEMLHTKNPSAEYVKLARDVLNEFFGIDSQAIYGKNIMYGASFNVDSKDIEVGFISGISQTTNTILSAFIVLSFAISIIILVVITHIMITSSQRSIAIFSILGYSNKEKVFLFFFNFIPIIVLACLFMIPVTYGFITVLNKFMMATSQIVLPIKLYVSTVLLSLAVCLSIFTITSIITWNSINKAKAIDVLKGK
>LR214940.1|VEU56011.1|683832_684867_+|ABC-transporter-ATP-binding-protein
MDKKIKNKSTPTKNTEEVKKTTRVAKTKKSSSNEKEKIVLDINSEISKGLSPIKLAKADKPFTIKDEDDNNVKVLDNKTLKKFKKANNKAWKKDGREHLKNQEGLIVEVEDVHKTYLSGNIATEVLKGVSFSIKAGEIAILYGKSGSGKSTLLNIISALDRPTSGKVIVNNVSLPYMSNEKQTLFRRKNISFIFQNYNLLQNLNSFDNVETGSYLQPVKEKRLNLKKLFRDFELTECMYKYPSQMSGGQQQRVSILRALAKNSPIIVADEPTGALDEKTSKIVLKILQQINKEYGATIIIVSHDPSVAEVADKVIYLENGHIREIIQQERKWLIDKNSSENSNE
>LR214940.1|VEU56012.1|685042_686050_+|transposase
MNYTHINYEERVLIEHYFNLNYSINKIAKEINRSASTISRELKRNFDSNIMNYNSNSASQLARKRRYHRFYFKFFSIREEYETFNDIFTNVYNKTIWTVELLHFAAKYLFKFKCPSLRTTFNWIKSGNWVLVHKDLLRKSYVKGGKRKNTTVVDRLVQSEYVIPYWARPKEINNRSEFGHWEIDLVCGKNARGHYHIITLVERQTRFAFTEKIKGKHPDNLNRILLKMIAKYNIPVFSITTDNGFEFRKLGLIGYKLGICIYQCDKYASWQKGSDENWNGLFRRFFPKGTDFNDILPEEIQRITTLINKRLRKTLNYKSAEELYVDAYNKTIESL
>LR214940.1|VEU56013.1|686192_686672_-|Uncharacterised-protein
MKDHNKKQPIKNLGDIIRKNTISKKDFISRISQYKNVKYFEMVFKKEKENLYFCAVCNVEDFKEDMPKKPFDPDQFAKDLILNVKVMIQNEIFPILEKIENRLDKIEARLDKVEARLDKVEARLDKVEKDITEIKKDVAMLKSFHIKDIEEYNKNMANN
>LR214940.1|VEU56014.1|686969_687641_+|translation-initiation-factor-IF-3
MDFVKTTFYFKEKVIQQNSNINSKLPKAEYIVNERIPYNKVFVIDENGEKIGVMLKTEAIEKAKESNMDLVLIALDNNRPITRIMDYGKFKYDKKKKNKLNKEKQSQTTNREIRLTPLIGDHDLKTKAKKAREFILDNARVKVSVKFRGREKTRTELGTEILDKFFAMVEDVAKITKPAMMVNDKFLDMFIEKDSKKVKSFNNSNKEDEDKIKEGEDNAKNED
>LR214940.1|VEU56015.1|687621_687810_+|50S-ribosomal-protein-L35
MPKMKTKSAIKKRVKITANGKVKRGNAYRSHLAQNKSTKQKRHSRKSSELSHSDFKRYKELI
>LR214940.1|VEU56016.1|687837_688212_+|50S-ribosomal-protein-L20
MRTKGGIVTRRRRNKWLKLAKGYWGHKSIGYKVAKQQVVKSWTYAFRDRKQIKREFRKLWIARINAAVRPLGVTYSRLIEGLKKANILINRKMLSELAINQPTVFNQIVEKAKKALVQKKAELK
>LR214940.1|VEU56017.1|688285_688480_+|Uncharacterised-protein
MYRPEDIDKMYKQEYEYKCVEYKNSQHFNENVNEEAAKGWRVVAVTYWVNFKALLMVTYERKLI
>LR214940.1|VEU56018.1|688486_689605_+|tRNA-specific-2-thiouridylase-mnmA
MERKKRVVIGLSGGIDSAISCYLLKNKGYEVIGLFMRNWDSFLNNDFLGNKTSFDDQCPQEKDYEDAKDVAKFFGIELHRVDFVKEYWDDVFSYLIEEYKKGRTPNPDIFCNKYIKFAKFADFAFNKLNADYIATGHYAKTKDCKLYKAFDDTKDQSYFLAQLSNKQLEKVIFPLDSITKKEVRLIANKLGLSIANKKDSTGICFIGERKFTEFLENYIPSKPGNIIDIATNKVVGKHIGAMYYTIGQRKGLNLGGYALPYFVVGHNLKDRILYVANERNKEYLLSNSLVAINTNFLETNFDKNNLTAKFRYRQKDIKIDLEILDNNKVLIKYDYLEAITPGQQVVVYEKDKVILGAIIDKVFYDGKEKTYI

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity
LR214940_1	1.1|240319|28|LR214940|CRISPRCasFinder	240319-240346	28	NZ_CP033120	Acinetobacter wuhouensis strain WCHAW010062 plasmid p11_010062, complete sequence	3310-3337	4	0.857
LR214940_1	1.1|240319|28|LR214940|CRISPRCasFinder	240319-240346	28	NZ_CP012975	Staphylococcus aureus strain ST20130943 plasmid pST20130943, complete sequence	12741-12768	6	0.786
LR214940_1	1.1|240319|28|LR214940|CRISPRCasFinder	240319-240346	28	NZ_CP047853	Staphylococcus aureus strain UP_274 plasmid unnamed, complete sequence	7248-7275	6	0.786
LR214940_1	1.1|240319|28|LR214940|CRISPRCasFinder	240319-240346	28	NZ_CP047836	Staphylococcus aureus strain UP_883 plasmid unnamed, complete sequence	16610-16637	6	0.786
LR214940_1	1.1|240319|28|LR214940|CRISPRCasFinder	240319-240346	28	NC_013550	Staphylococcus aureus plasmid pBORa53, complete sequence	7969-7996	6	0.786
LR214940_1	1.1|240319|28|LR214940|CRISPRCasFinder	240319-240346	28	AP013412	Uncultured Mediterranean phage uvMED DNA, complete genome, group G16, isolate: uvMED-CGR-C10A-MedDCM-OCT-S46-C61	24992-25019	6	0.786
LR214940_1	1.1|240319|28|LR214940|CRISPRCasFinder	240319-240346	28	NC_022111	Prevotella sp. oral taxon 299 str. F0039 plasmid, complete sequence	609751-609778	6	0.786
LR214940_1	1.1|240319|28|LR214940|CRISPRCasFinder	240319-240346	28	MN693005	Marine virus AFVG_117M50, complete genome	55465-55492	6	0.786
LR214940_1	1.1|240319|28|LR214940|CRISPRCasFinder	240319-240346	28	LC377538	Staphylococcus aureus plasmid pNTUH_3874 NTUH_3874 DNA, complete sequence	40669-40696	7	0.75
LR214940_1	1.1|240319|28|LR214940|CRISPRCasFinder	240319-240346	28	NC_013940	Deferribacter desulfuricans SSM1 megaplasmid pDF308, complete sequence	120487-120514	7	0.75
LR214940_1	1.1|240319|28|LR214940|CRISPRCasFinder	240319-240346	28	MK327941	Escherichia phage vB_EcoM_G37-3, complete genome	57466-57493	7	0.75
LR214940_1	1.1|240319|28|LR214940|CRISPRCasFinder	240319-240346	28	NC_011836	Clostridium kluyveri NBRC 12016 plasmid pCKL1, complete sequence	35699-35726	8	0.714
LR214940_1	1.1|240319|28|LR214940|CRISPRCasFinder	240319-240346	28	NC_009466	Clostridium kluyveri DSM 555 plasmid pCKL555A, complete sequence	10876-10903	8	0.714

1. spacer 1.1|240319|28|LR214940|CRISPRCasFinder matches to NZ_CP033120 (Acinetobacter wuhouensis strain WCHAW010062 plasmid p11_010062, complete sequence) position: , mismatch: 4, identity: 0.857

ttacgaagttaaaaataatat--attgcca	CRISPR spacer
ttactaagttaaaaataatatcaatttc--	Protospacer
**** ****************  *** *  

2. spacer 1.1|240319|28|LR214940|CRISPRCasFinder matches to NZ_CP012975 (Staphylococcus aureus strain ST20130943 plasmid pST20130943, complete sequence) position: , mismatch: 6, identity: 0.786

ttacgaagttaaaaataatatattgcca	CRISPR spacer
atacgaagttaaaaaaaatatatgactc	Protospacer
 ************** ******* .*. 

3. spacer 1.1|240319|28|LR214940|CRISPRCasFinder matches to NZ_CP047853 (Staphylococcus aureus strain UP_274 plasmid unnamed, complete sequence) position: , mismatch: 6, identity: 0.786

ttacgaagttaaaaataatatattgcca	CRISPR spacer
atacgaagttaaaaaaaatatatgactc	Protospacer
 ************** ******* .*. 

4. spacer 1.1|240319|28|LR214940|CRISPRCasFinder matches to NZ_CP047836 (Staphylococcus aureus strain UP_883 plasmid unnamed, complete sequence) position: , mismatch: 6, identity: 0.786

ttacgaagttaaaaataatatattgcca	CRISPR spacer
atacgaagttaaaaaaaatatatgactc	Protospacer
 ************** ******* .*. 

5. spacer 1.1|240319|28|LR214940|CRISPRCasFinder matches to NC_013550 (Staphylococcus aureus plasmid pBORa53, complete sequence) position: , mismatch: 6, identity: 0.786

ttacgaagttaaaaataatatattgcca	CRISPR spacer
atacgaagttaaaaaaaatatatgactc	Protospacer
 ************** ******* .*. 

6. spacer 1.1|240319|28|LR214940|CRISPRCasFinder matches to AP013412 (Uncultured Mediterranean phage uvMED DNA, complete genome, group G16, isolate: uvMED-CGR-C10A-MedDCM-OCT-S46-C61) position: , mismatch: 6, identity: 0.786

ttacgaagttaaaaataatatattgcca	CRISPR spacer
caggggagttaaaaataatataatgcca	Protospacer
. . *.**************** *****

7. spacer 1.1|240319|28|LR214940|CRISPRCasFinder matches to NC_022111 (Prevotella sp. oral taxon 299 str. F0039 plasmid, complete sequence) position: , mismatch: 6, identity: 0.786

ttacgaagttaaaaataatatattgcca	CRISPR spacer
ttacaaagttagaaataatatatataaa	Protospacer
****.******.***********    *

8. spacer 1.1|240319|28|LR214940|CRISPRCasFinder matches to MN693005 (Marine virus AFVG_117M50, complete genome) position: , mismatch: 6, identity: 0.786

ttacgaagttaaaaataatatattgcca	CRISPR spacer
gtacgaagataaaaattatatatttcat	Protospacer
 ******* ******* ******* *  

9. spacer 1.1|240319|28|LR214940|CRISPRCasFinder matches to LC377538 (Staphylococcus aureus plasmid pNTUH_3874 NTUH_3874 DNA, complete sequence) position: , mismatch: 7, identity: 0.75

ttacgaagttaaaaataatatattgcca	CRISPR spacer
gtacgaagttaacaataatattttagtt	Protospacer
 *********** ******** **. . 

10. spacer 1.1|240319|28|LR214940|CRISPRCasFinder matches to NC_013940 (Deferribacter desulfuricans SSM1 megaplasmid pDF308, complete sequence) position: , mismatch: 7, identity: 0.75

ttacgaagttaaaaataatatattgcca	CRISPR spacer
cttcgaagttaaaattaatatattttgt	Protospacer
.* *********** ********* .  

11. spacer 1.1|240319|28|LR214940|CRISPRCasFinder matches to MK327941 (Escherichia phage vB_EcoM_G37-3, complete genome) position: , mismatch: 7, identity: 0.75

ttacgaagttaaaaataatatattgcca	CRISPR spacer
ctacgaagttaaaaaaaacatattcttg	Protospacer
.************** **.***** ...

12. spacer 1.1|240319|28|LR214940|CRISPRCasFinder matches to NC_011836 (Clostridium kluyveri NBRC 12016 plasmid pCKL1, complete sequence) position: , mismatch: 8, identity: 0.714

ttacgaagttaaaaataatatattgcca	CRISPR spacer
aggcgaagttaaaaataatatatgtgag	Protospacer
  .********************    .

13. spacer 1.1|240319|28|LR214940|CRISPRCasFinder matches to NC_009466 (Clostridium kluyveri DSM 555 plasmid pCKL555A, complete sequence) position: , mismatch: 8, identity: 0.714

ttacgaagttaaaaataatatattgcca	CRISPR spacer
aggcgaagttaaaaataatatatgtgag	Protospacer
  .********************    .