CRISPRimmunity

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Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Target MGE spacer number	Prophage number
NZ_CP019369	Borrelia turicatae 91E135 isolate Oz1 plasmid lpU43, complete sequence	0 crisprs	NA	0	0
NZ_CP019363	Borrelia turicatae 91E135 isolate Oz1 plasmid cpD, complete sequence	0 crisprs	NA	0	0
NZ_CP019364	Borrelia turicatae 91E135 isolate Oz1 plasmid lpB34, complete sequence	0 crisprs	NA	0	0
NZ_CP019368	Borrelia turicatae 91E135 isolate Oz1 plasmid lpT39, complete sequence	1 crisprs	NA	1	0
NZ_CP019366	Borrelia turicatae 91E135 isolate Oz1 plasmid lpF33.5, complete sequence	0 crisprs	NA	0	0
NZ_CP019367	Borrelia turicatae 91E135 isolate Oz1 plasmid lpG29, complete sequence	0 crisprs	NA	0	0
NZ_CP019362	Borrelia turicatae 91E135 isolate Oz1 plasmid cp31, complete sequence	0 crisprs	NA	0	0
NC_008710	Borrelia turicatae 91E135, complete sequence	0 crisprs	NA	0	1
NZ_CP019365	Borrelia turicatae 91E135 isolate Oz1 plasmid lpE43, complete sequence	1 crisprs	NA	1	0

Results visualization

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CRISPR-Cas detection and classification

Crispr_ID: NZ_CP019368_1

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

NZ_CP019368_1

4231-4308

Orphan

Consensus_repeat	Method
TTCACTTCTAACGTTATCAATCTT	CRISPRCasFinder

1 spacers

The CRISPR arrays of NZ_CP019368_1

>merge|NZ_CP019368|1|4231-4308|CRISPRCasFinder
TTCACTTCTAACGTTATCAATCTTAGTATCGAGGTCCTTAATGTCTGATTTCAGTTCACTTCTAACGTTGTCAATCTT

>NZ_CP019368|1|1|4231-4308|CRISPRCasFinder
TTCACTTCTAACGTTATCAATCTT	AGTATCGAGGTCCTTAATGTCTGATTTCAG
TTCACTTCTAACGTTGTCAATCTT

Protein	Signature genes	Signature genes Name	Protein_function
NZ_CP019368.1\|WP_088895220.1\|12170_12596_-\|Mlp-family-lipoprotein	unknown	unknown	gnl\|CDD\|308750
NZ_CP019368.1\|WP_088895222.1\|13117_13705_+\|hypothetical-protein	unknown	unknown	gnl\|CDD\|273684
NZ_CP019368.1\|WP_088895218.1\|9826_10924_-\|hypothetical-protein	unknown	unknown	gnl\|CDD\|367076
NZ_CP019368.1\|WP_088895237.1\|2835_3849_-\|variable-large-family-protein	unknown	unknown	gnl\|CDD\|376417
NZ_CP019368.1\|WP_181005563.1\|8516_9278_-\|ParA-family-protein	unknown	unknown	gnl\|CDD\|224113
NZ_CP019368.1\|WP_161491440.1\|4897_5053_-\|hypothetical-protein	unknown	unknown	unknown
NZ_CP019368.1\|WP_088895219.1\|11143_12076_+\|hypothetical-protein	unknown	unknown	gnl\|CDD\|200235
NZ_CP019368.1\|WP_088895221.1\|12762_12981_+\|DUF603-domain-containing-protein	unknown	unknown	gnl\|CDD\|282496
NZ_CP019368.1\|WP_088895217.1\|9253_9817_-\|DUF226-domain-containing-protein	unknown	unknown	gnl\|CDD\|308504
NZ_CP019368.1\|WP_145954726.1\|5289_6195_-\|variable-large-family-protein	unknown	unknown	gnl\|CDD\|376417
NZ_CP019368.1\|WP_088895216.1\|7855_8437_-\|chromosome-replication/partitioning-protein	unknown	unknown	gnl\|CDD\|376591
NZ_CP019368.1\|WP_161491439.1\|2023_2302_+\|hypothetical-protein	unknown	unknown	unknown

Protein	Function_ID	Function_description	E-value
NZ_CP019368.1\|WP_088895218.1\|9826_10924_-\|hypothetical-protein	gnl\|CDD\|367076	pfam02414, Borrelia_orfA, Borrelia ORF-A. This protein is encoded by an open reading frame in plasmid borne DNA repeats of Borrelia species. This protein is known as ORF-A. The function of this putative protein is unknown.	1.60064e-61
NZ_CP019368.1\|WP_088895220.1\|12170_12596_-\|Mlp-family-lipoprotein	gnl\|CDD\|308750	pfam03304, Mlp, Mlp lipoprotein family. The Mlp (for Multicopy Lipoprotein) family of lipoproteins is found in Borrelia species. This family were previously known as 2.9 lipoprotein genes. These surface expressed genes may represent new candidate vaccinogens for Lyme disease. Members of this family generally are downstream of four ORFs called A,B,C and D that are involved in hemolytic activity.	1.04512e-23
NZ_CP019368.1\|WP_088895222.1\|13117_13705_+\|hypothetical-protein	gnl\|CDD\|273684	TIGR01547, Uncharacterized_protein_YqaT, phage terminase, large subunit, PBSX family. This model detects members of a highly divergent family of the large subunit of phage terminase. All members are encoded by phage genomes or within prophage regions of bacterial genomes. This is a distinct family from pfam03354. [Mobile and extrachromosomal element functions, Prophage functions].	1.63772e-21
NZ_CP019368.1\|WP_181005563.1\|8516_9278_-\|ParA-family-protein	gnl\|CDD\|224113	COG1192, Soj, ATPases involved in chromosome partitioning [Cell division and chromosome partitioning].	7.42062e-43
NZ_CP019368.1\|WP_088895237.1\|2835_3849_-\|variable-large-family-protein	gnl\|CDD\|376417	pfam00921, Lipoprotein_2, Borrelia lipoprotein. This family of lipoproteins is found in Borrelia spirochetes. The function of these proteins is uncertain.	3.15464e-59
NZ_CP019368.1\|WP_088895219.1\|11143_12076_+\|hypothetical-protein	gnl\|CDD\|200235	TIGR03033, hypothetical_protein, putative phage-type endonuclease. Members of this protein family are found often in phage genomes and in prokaryotic genomes in uncharacterized regions that resemble integrated prophage regions.	1.47755e-06
NZ_CP019368.1\|WP_145954726.1\|5289_6195_-\|variable-large-family-protein	gnl\|CDD\|376417	pfam00921, Lipoprotein_2, Borrelia lipoprotein. This family of lipoproteins is found in Borrelia spirochetes. The function of these proteins is uncertain.	4.19136e-49
NZ_CP019368.1\|WP_088895216.1\|7855_8437_-\|chromosome-replication/partitioning-protein	gnl\|CDD\|376591	pfam01672, Plasmid_parti, Putative plasmid partition protein. This family consists of conserved hypothetical proteins from Borrelia burgdorferi the lyme disease spirochaete, some of which are putative plasmid partition proteins.	1.56658e-31
NZ_CP019368.1\|WP_088895217.1\|9253_9817_-\|DUF226-domain-containing-protein	gnl\|CDD\|308504	pfam02890, DUF226, Borrelia family of unknown function DUF226. This family of proteins are found in Borrelia. The proteins are about 190 amino acids long and have no known function.	2.98894e-56
NZ_CP019368.1\|WP_088895221.1\|12762_12981_+\|DUF603-domain-containing-protein	gnl\|CDD\|282496	pfam04645, DUF603, Protein of unknown function, DUF603. This family includes several uncharacterized proteins from Borrelia species.	1.85906e-17

>NZ_CP019368.1|WP_088895237.1|2835_3849_-|variable-large-family-protein
MTLFLLLSCGSGSAKTEDPKTLFLTSIANLGKGFLDVFTSLSDMVAGAFGIKADTKKSEVGKYFNDIANTMNTVKAKLNDVVAKNDNYPKVKSVVDTFITNTLDKIADGAKIAATGATGSDAIGNAVKDTDAAPANKDAVNTLVKGIKTIVGVVLKDNEGNPDATKTSGEKNKVVGKLLGGKANAEEAQAAVASASIGAVTGADILQAIANSGEAGTGEIKIEEAKNAADIAAAKKDDNKDFGASAQKDAVIAAGIALRAMAKDGKFAANNDDKYANAVNGVAASAVGKTLSTLIIAIRNTVDTGLKSISEALAAVKQEDKSADSTTPADATVSGKQ
>NZ_CP019368.1|WP_161491439.1|2023_2302_+|hypothetical-protein
MLTSILLSRSLISDFNSFLTVSILLSSSLNLVSILVLRLFSTVLILVSSSLNLASILVSSSVLTDLISACKRASTFSSFISKFSLNTQYLYK
>NZ_CP019368.1|WP_161491440.1|4897_5053_-|hypothetical-protein
MTKTFLNLRARMSHKFRIAPIKPISNKFTKIATLIEPFATSKQMVKVMMIH
>NZ_CP019368.1|WP_145954726.1|5289_6195_-|variable-large-family-protein
MVGGVLGFDTNTKKSDVGNYFKKIQETVQGTKDKLNKIVADMKSENNPNASITETAVNKLVSETLSKIIDGAKIASEAIGSDNNPIANVATAGNDAAAGGVKGDGIENLVKGIKDLVEVILGDKGNAEAGDANKASDGGSRTANAADGEAGKLFATGNGPAGDQNNSKKVATDAAKAVGAVTGADILQAMVKDGGAATLAKNSAEQVAGANAKDSVIAGGIVLRAIAKGGKFANGNNAGNADVATVIKGAAISAVTKALGTLTIAIRNTIDVGLKEVKGAIKINATDTPVTTDNQTPETKS
>NZ_CP019368.1|WP_088895216.1|7855_8437_-|chromosome-replication/partitioning-protein
MNKKNNDEIIINARDLNNDKNSILLTNDDINVSDDQKRFKLLKSKLKDNIKDDIYNKIEAMHILREIKEKEYYKLDGYKSFSQFIKDYKLAKSQAYSYLRIASAIQDGILKEEYLVENGFRQSLSFLAEKESTSIKKSRVNSIKPLRFQLKNQDSYAFYKQNAKFTNFLMDELFKDKKDLLEEFMKKFKSLKG
>NZ_CP019368.1|WP_181005563.1|8516_9278_-|ParA-family-protein
MDRKKPKIITLASIKGGVGKSTSSIIFATLLAQKYKVLLIDMDTQASVTSYFFNEIKEKNINLMEKNICEVLKGNLEIANAIFNIEGSLDLLPSYLTLHSLNEDFYCANRHKSIDLKLKVELRRLKVKYDYIVIDTNPSLDFTLKCSLNSTDYIIVPMTAEKWTIESYELLEFFIKKLERLIPIFFIITRFKKNNTHKKLLKIIQAKDTFLGIVSERENLNKRIASNSNFDLKSDYIIEYDQILSNLLYRISC
>NZ_CP019368.1|WP_088895217.1|9253_9817_-|DUF226-domain-containing-protein
MQSVLERLKDKKLEIKDKVKSRGLFTKIEEIDNKTIYHTKVMNDLYTFGVHRRQNNKFFIAFRGLFNQEKISTINLFSIKGDDKFLGICYGYRKPVQNIITKYEENGVIRSYTFSKVYYIEFRFKKGSVFCYIKGISRLIKQEKSETQYSQFLLELIINLEEQVYKFYGKKLPEGGIITKWIEKNLK
>NZ_CP019368.1|WP_088895218.1|9826_10924_-|hypothetical-protein
MRSTKKPTNKHQHKLIVLISTLNYMNLNIQQYTQSNILYYFNNNMKKNDQKPIKLKTLQSYLYKLEKEFKVTINYHRHLGVNMGTEIHYKLKYSKKECHRIINKHFRDKKEERHKKRVSAYIKKTCIQNSNVEKWECFNNSYNKKEEDKNNIKSMERLQVEKYAKKCNFKSNAFFSILNLKADKDFKIQALKAIKIAENSGYEKINDTKPNNTKLESKQKELSKILNETKANLKNEGYNSKQLETQIQNFYEQYKNKPHFIIEKDKYSDLEKIIEKIKKTIEYTNKNTKENEKDTRNNIFSVLLEQLRSKVNISVLVPILKNYLNKQNKLEYRKVFSNHYYYELLELIINNKNYLQLEESKKITS
>NZ_CP019368.1|WP_088895219.1|11143_12076_+|hypothetical-protein
MHIRVKKIFKDKQKQVSKISRIGRKLPKIGKDECFKFNRKVDFSMQRKVLKRIGVGSMFIGADSLEKLMRERILKAIGREIPFEDNLSMRKGNELENLGFREFVRIHASNIKMLHKNKYANGVDKYNYFKKFKDRDTLVGLIIDRWFLGSGGESELLEIKIIDNFYLKNAAVEYNKTGNFLENKYFFKYYVQVQMHLLCTGLNKGNLFFIIGGELVNCVIERDNNFISDVMVNVSRLEQEVSRIAKSLKSKSDIDIENIELDELSVIIGNFLKNSFVYQDLFDIDFKFDFYSYSYHFYNEFNLFSLSLFI
>NZ_CP019368.1|WP_088895220.1|12170_12596_-|Mlp-family-lipoprotein
MNKINFILVLLLLISSCEYEHGNATPKSRVKRNLEEQSEVQKTPEEALREKLNDTEKTNLDFLKEALGNESKFSQFLSFDEEKIKAALKHINDELAKCNGNNEGKNGFKAVVQGYFSKIDNDTLNGFKENATSTCQAGGSG
>NZ_CP019368.1|WP_088895221.1|12762_12981_+|DUF603-domain-containing-protein
MSRVKKSFDDYIVYFREGKLNDASIAKEMGVSRANVGKMRRRWEEIKDNPEYMTGTSKLTICGDTFNAMIGT
>NZ_CP019368.1|WP_088895222.1|13117_13705_+|hypothetical-protein
MLKIPFRPKFSNTSYFEIDSLKVNLYGGDRANDFERFRGSNSALIYVNEATTLHKETLIECLKRLRVGMQTIIFDTNPDSSEHFFKTDYIDNTKIYSTYNFTTYDNELISREFIKTQEEIYRDIPTYKAKVLLGEWVASNDAIFTNVNLTSNHEFRVPIAYLDSAYNIGGDNTAICVLERVDQSYYAFIFQEKLL

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Click the colored protein region to show detailed information

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity
NZ_CP019368_1	1.1\|4255\|30\|NZ_CP019368\|CRISPRCasFinder	4255-4284	30	NZ_CP019365	Borrelia turicatae 91E135 isolate Oz1 plasmid lpE43, complete sequence	12042-12071	0	1.0
NZ_CP019368_1	1.1\|4255\|30\|NZ_CP019368\|CRISPRCasFinder	4255-4284	30	NZ_CP019368	Borrelia turicatae 91E135 isolate Oz1 plasmid lpT39, complete sequence	4255-4284	0	1.0
NZ_CP019368_1	1.1\|4255\|30\|NZ_CP019368\|CRISPRCasFinder	4255-4284	30	NZ_CP015636	Borrelia turicatae strain BTE5EL plasmid lp48	1725-1754	2	0.933
NZ_CP019368_1	1.1\|4255\|30\|NZ_CP019368\|CRISPRCasFinder	4255-4284	30	NZ_CP015632	Borrelia turicatae strain BTE5EL plasmid lp30	21348-21377	5	0.833
NZ_CP019368_1	1.1\|4255\|30\|NZ_CP019368\|CRISPRCasFinder	4255-4284	30	NZ_CP015632	Borrelia turicatae strain BTE5EL plasmid lp30	21294-21323	6	0.8
NZ_CP019368_1	1.1\|4255\|30\|NZ_CP019368\|CRISPRCasFinder	4255-4284	30	NC_047956	Pseudomonas phage Achelous, complete genome	24812-24841	6	0.8

1. spacer 1.1|4255|30|NZ_CP019368|CRISPRCasFinder matches to NZ_CP019365 (Borrelia turicatae 91E135 isolate Oz1 plasmid lpE43, complete sequence) position: , mismatch: 0, identity: 1.0

agtatcgaggtccttaatgtctgatttcag	CRISPR spacer
agtatcgaggtccttaatgtctgatttcag	Protospacer
******************************

2. spacer 1.1|4255|30|NZ_CP019368|CRISPRCasFinder matches to NZ_CP019368 (Borrelia turicatae 91E135 isolate Oz1 plasmid lpT39, complete sequence) position: , mismatch: 0, identity: 1.0

agtatcgaggtccttaatgtctgatttcag	CRISPR spacer
agtatcgaggtccttaatgtctgatttcag	Protospacer
******************************

3. spacer 1.1|4255|30|NZ_CP019368|CRISPRCasFinder matches to NZ_CP015636 (Borrelia turicatae strain BTE5EL plasmid lp48) position: , mismatch: 2, identity: 0.933

agtatcgaggtccttaatgtctgatttcag	CRISPR spacer
ggaatcgaggtccttaatgtctgatttcag	Protospacer
.* ***************************

4. spacer 1.1|4255|30|NZ_CP019368|CRISPRCasFinder matches to NZ_CP015632 (Borrelia turicatae strain BTE5EL plasmid lp30) position: , mismatch: 5, identity: 0.833

agtatcgaggtccttaatgtctgatttcag	CRISPR spacer
attatccaggtctttaatgtctgattttaa	Protospacer
* **** *****.**************.*.

5. spacer 1.1|4255|30|NZ_CP019368|CRISPRCasFinder matches to NZ_CP015632 (Borrelia turicatae strain BTE5EL plasmid lp30) position: , mismatch: 6, identity: 0.8

agtatcgaggtccttaatgtctgatttcag	CRISPR spacer
gttatctaggtctttaatgtctgattttaa	Protospacer
. **** *****.**************.*.

6. spacer 1.1|4255|30|NZ_CP019368|CRISPRCasFinder matches to NC_047956 (Pseudomonas phage Achelous, complete genome) position: , mismatch: 6, identity: 0.8

agtatcgaggtccttaatgtctgatttcag	CRISPR spacer
aatagcgaggtgcttaatgtctgatgtaaa	Protospacer
*.** ****** ************* * *.

Prophage detection

Region	Region Position	Protein_number	Hit_taxonomy	Key_proteins	Att_site	Prophage annotation

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

448138 : 457404

Streptococcus_phage(28.57%)

The bacterium proteins that are colored denote the protein is present at specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin' and 'tRNA')

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_011772374.1\|448138_448813_-	16S rRNA (cytidine(1402)-2'-O)-methyltransferase	M1PLC5	Streptococcus_phage	1.5e-34	41.5
WP_011772375.1\|449082_449403_+	hypothetical protein	NA	NA	NA	NA
WP_041178474.1\|449412_449802_-	DUF1893 domain-containing protein	NA	NA	NA	NA
WP_011772377.1\|449916_450495_+	YigZ family protein	A0A1X9I5T8	Streptococcus_phage	1.8e-15	32.7
WP_011772378.1\|450686_451439_+	ParA family protein	H7BUL8	unidentified_phage	3.7e-21	31.1
WP_011772379.1\|451441_452146_+	hypothetical protein	A0A1P8CWU6	Bacillus_phage	1.5e-29	46.6
WP_041178475.1\|452138_452921_-	ParB/RepB/Spo0J family partition protein	I3NLC2	Bifidobacterium_phage	2.5e-12	30.4
WP_041178476.1\|453055_455488_-	DNA topoisomerase (ATP-hydrolyzing) subunit A	G3M9Z5	Bacillus_virus	2.1e-102	32.6
WP_041178477.1\|455499_457404_-	DNA topoisomerase (ATP-hydrolyzing) subunit B	G3M9Z3	Bacillus_virus	7.3e-135	43.1

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

3. NZ_CP019365

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Crispr_ID: NZ_CP019365_1

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

NZ_CP019365_1

12018-12095

Orphan

Consensus_repeat	Method
AAGATTGACAACGTTAGAAGTGAA	CRISPRCasFinder

1 spacers

The CRISPR arrays of NZ_CP019365_1

>merge|NZ_CP019365|1|12018-12095|CRISPRCasFinder
AAGATTGACAACGTTAGAAGTGAACTGAAATCAGACATTAAGGACCTCGATACTAAGATTGATAACGTTAGAAGTGAA

>NZ_CP019365|1|1|12018-12095|CRISPRCasFinder
AAGATTGACAACGTTAGAAGTGAA	CTGAAATCAGACATTAAGGACCTCGATACT
AAGATTGATAACGTTAGAAGTGAA

Protein	Signature genes	Signature genes Name	Protein_function
NZ_CP019365.1\|WP_161491421.1\|18679_18841_-\|hypothetical-protein	unknown	unknown	unknown
NZ_CP019365.1\|WP_161491419.1\|15295_15475_-\|hypothetical-protein	unknown	unknown	unknown
NZ_CP019365.1\|WP_088895141.1\|13562_14171_+\|hypothetical-protein	unknown	unknown	gnl\|CDD\|366644
NZ_CP019365.1\|WP_088895135.1\|3417_4065_+\|hypothetical-protein	unknown	unknown	gnl\|CDD\|366644
NZ_CP019365.1\|WP_161491417.1\|9714_9930_+\|variable-large-family-protein	unknown	unknown	gnl\|CDD\|376417
NZ_CP019365.1\|WP_088895147.1\|20551_21163_+\|hypothetical-protein	unknown	unknown	gnl\|CDD\|366644
NZ_CP019365.1\|WP_088895157.1\|8483_9494_+\|variable-large-family-protein	unknown	unknown	gnl\|CDD\|376417
NZ_CP019365.1\|WP_145954709.1\|6518_7058_+\|vsp-protein	unknown	unknown	gnl\|CDD\|366644
NZ_CP019365.1\|WP_161491429.1\|14483_14876_+\|variable-large-family-protein	unknown	unknown	gnl\|CDD\|376417
NZ_CP019365.1\|WP_088895133.1\|1530_2628_-\|hypothetical-protein	unknown	unknown	gnl\|CDD\|367076
NZ_CP019365.1\|WP_145954713.1\|17913_18228_+\|variable-large-family-protein	unknown	unknown	gnl\|CDD\|376417
NZ_CP019365.1\|WP_088895138.1\|10043_11039_+\|variable-large-family-protein	unknown	unknown	gnl\|CDD\|376417
NZ_CP019365.1\|WP_088895136.1\|4267_5257_+\|variable-large-family-protein	unknown	unknown	gnl\|CDD\|376417
NZ_CP019365.1\|WP_145954714.1\|19445_20444_+\|variable-large-family-protein	unknown	unknown	gnl\|CDD\|376417
NZ_CP019365.1\|WP_161491418.1\|11273_11429_+\|hypothetical-protein	unknown	unknown	unknown
NZ_CP019365.1\|WP_161491428.1\|7377_8334_+\|variable-large-family-protein	unknown	unknown	gnl\|CDD\|376417
NZ_CP019365.1\|WP_088895140.1\|12446_13496_+\|variable-large-family-protein	unknown	unknown	gnl\|CDD\|376417
NZ_CP019365.1\|WP_161491420.1\|17581_17743_+\|hypothetical-protein	unknown	unknown	unknown
NZ_CP019365.1\|WP_088895143.1\|16179_17268_+\|variable-large-family-protein	unknown	unknown	gnl\|CDD\|376417
NZ_CP019365.1\|WP_161491427.1\|5412_6378_+\|variable-large-family-protein	unknown	unknown	gnl\|CDD\|376417

Protein	Function_ID	Function_description	E-value
NZ_CP019365.1\|WP_088895141.1\|13562_14171_+\|hypothetical-protein	gnl\|CDD\|366644	pfam01441, Lipoprotein_6, Lipoprotein. Members of this family are lipoproteins that are probably involved in evasion of the host immune system by pathogens.	2.40106e-31
NZ_CP019365.1\|WP_088895135.1\|3417_4065_+\|hypothetical-protein	gnl\|CDD\|366644	pfam01441, Lipoprotein_6, Lipoprotein. Members of this family are lipoproteins that are probably involved in evasion of the host immune system by pathogens.	1.2488e-53
NZ_CP019365.1\|WP_161491417.1\|9714_9930_+\|variable-large-family-protein	gnl\|CDD\|376417	pfam00921, Lipoprotein_2, Borrelia lipoprotein. This family of lipoproteins is found in Borrelia spirochetes. The function of these proteins is uncertain.	7.84586e-05
NZ_CP019365.1\|WP_088895157.1\|8483_9494_+\|variable-large-family-protein	gnl\|CDD\|376417	pfam00921, Lipoprotein_2, Borrelia lipoprotein. This family of lipoproteins is found in Borrelia spirochetes. The function of these proteins is uncertain.	2.07187e-60
NZ_CP019365.1\|WP_145954709.1\|6518_7058_+\|vsp-protein	gnl\|CDD\|366644	pfam01441, Lipoprotein_6, Lipoprotein. Members of this family are lipoproteins that are probably involved in evasion of the host immune system by pathogens.	1.82654e-41
NZ_CP019365.1\|WP_161491429.1\|14483_14876_+\|variable-large-family-protein	gnl\|CDD\|376417	pfam00921, Lipoprotein_2, Borrelia lipoprotein. This family of lipoproteins is found in Borrelia spirochetes. The function of these proteins is uncertain.	6.36972e-10
NZ_CP019365.1\|WP_088895133.1\|1530_2628_-\|hypothetical-protein	gnl\|CDD\|367076	pfam02414, Borrelia_orfA, Borrelia ORF-A. This protein is encoded by an open reading frame in plasmid borne DNA repeats of Borrelia species. This protein is known as ORF-A. The function of this putative protein is unknown.	9.06006e-67
NZ_CP019365.1\|WP_145954713.1\|17913_18228_+\|variable-large-family-protein	gnl\|CDD\|376417	pfam00921, Lipoprotein_2, Borrelia lipoprotein. This family of lipoproteins is found in Borrelia spirochetes. The function of these proteins is uncertain.	4.84327e-17
NZ_CP019365.1\|WP_088895138.1\|10043_11039_+\|variable-large-family-protein	gnl\|CDD\|376417	pfam00921, Lipoprotein_2, Borrelia lipoprotein. This family of lipoproteins is found in Borrelia spirochetes. The function of these proteins is uncertain.	1.65882e-29
NZ_CP019365.1\|WP_088895143.1\|16179_17268_+\|variable-large-family-protein	gnl\|CDD\|376417	pfam00921, Lipoprotein_2, Borrelia lipoprotein. This family of lipoproteins is found in Borrelia spirochetes. The function of these proteins is uncertain.	1.54969e-29
NZ_CP019365.1\|WP_145954714.1\|19445_20444_+\|variable-large-family-protein	gnl\|CDD\|376417	pfam00921, Lipoprotein_2, Borrelia lipoprotein. This family of lipoproteins is found in Borrelia spirochetes. The function of these proteins is uncertain.	1.14848e-55
NZ_CP019365.1\|WP_088895136.1\|4267_5257_+\|variable-large-family-protein	gnl\|CDD\|376417	pfam00921, Lipoprotein_2, Borrelia lipoprotein. This family of lipoproteins is found in Borrelia spirochetes. The function of these proteins is uncertain.	8.25069e-39
NZ_CP019365.1\|WP_161491428.1\|7377_8334_+\|variable-large-family-protein	gnl\|CDD\|376417	pfam00921, Lipoprotein_2, Borrelia lipoprotein. This family of lipoproteins is found in Borrelia spirochetes. The function of these proteins is uncertain.	2.31615e-60
NZ_CP019365.1\|WP_088895140.1\|12446_13496_+\|variable-large-family-protein	gnl\|CDD\|376417	pfam00921, Lipoprotein_2, Borrelia lipoprotein. This family of lipoproteins is found in Borrelia spirochetes. The function of these proteins is uncertain.	1.97121e-63
NZ_CP019365.1\|WP_088895147.1\|20551_21163_+\|hypothetical-protein	gnl\|CDD\|366644	pfam01441, Lipoprotein_6, Lipoprotein. Members of this family are lipoproteins that are probably involved in evasion of the host immune system by pathogens.	1.19359e-49
NZ_CP019365.1\|WP_161491427.1\|5412_6378_+\|variable-large-family-protein	gnl\|CDD\|376417	pfam00921, Lipoprotein_2, Borrelia lipoprotein. This family of lipoproteins is found in Borrelia spirochetes. The function of these proteins is uncertain.	2.05747e-30

>NZ_CP019365.1|WP_161491418.1|11273_11429_+|hypothetical-protein
MTKTFLNLRVRMSHKFRIAPIKPISNKFTKIATLIEPFATSKQMVKVMMIH
>NZ_CP019365.1|WP_088895138.1|10043_11039_+|variable-large-family-protein
MATESKISFFDSLIKIGHGFQEIFGVFGNAIGDAFGFTAVKSGDKRSKVGEHFDKIRKGLEDTKGKLDGLVKDITSTPHADTTGVEATIKSASDVIVKLIDSVTKLAGATGDTDISDGAGANAVAVGAEVASVKTVIEEVKAIIDVADKSGVKVKAGNAGVQVAGGAATAGAALVGKADAAAPAAGAGSKLADEVTKADSWAMIDKIKNAKFEGALAAGDNNEAGVLAVGIAGNGGDNHNGAKTNADLVAAVALKAMTKGGKFSAANDDAEAVKAAAASAVNKVLGVLDIIIKKTVESELDKVGEAVKGIKYSETTIEATEATTIQPAAAK
>NZ_CP019365.1|WP_161491417.1|9714_9930_+|variable-large-family-protein
MEFAKGGQADHVSHSADAKAVQSAGVTAVTKLLVAAEDLIKKTVKNILKIAKEKIDEARSGKGMINLKEKE
>NZ_CP019365.1|WP_088895157.1|8483_9494_+|variable-large-family-protein
MTLFLLLSCGSGSSKVEDPKTLFLTSIANLGKGFLDVFTSFSDMVAGAFGIKAETKKSDIGKYFTDIENTMNTVKAKLNDVVATNGNYPKIKEVVNKFIAGILDKISDGAKIAASGAGDNSTIGDATVDKDAVHADAASVNALVKGIKTIVDVVLKGKGDASANATKDEGEDKKYIGKLFSEVKANAAEAKEATAASASIGAVSGADILQAIVKSGNVDNSKNIEDATDAASIAAAKKDNGKKEIKNDAKKDAVIAGGIALRAMAKGGQFAIKSGENDAVPIVNRAAASAVSKTLGTLMLAIRNTVDTGLKSISEALAAVKQEDMPVETSESGTGK
>NZ_CP019365.1|WP_161491428.1|7377_8334_+|variable-large-family-protein
MANLGKGFLDVFTSLSDMVAGAFGIKADTKKSDIGQYFTDIEKTMTSVKEKLQTEVAKNGNYLKVKSVVDTFITETLDKIAEGAKTAAKGATGDVIGNATATGHGATPASKDSVVSLVKGIKTIVEVVLKKDEGNAGATKTGEDKKDVGKLFADDNGKADAKEENIAKASASVGSVTGADILQAIAKSKEDPQVDNANGIEKATDAAEISIAPAVNDKKEIKEAEAKKDAVIAAGIALRAMAKDGKFAANNNAKDADAVNGVAANAVGKTLSTLIIAIRNTVDTGLKSISDVLATVTQEDKAVDSITPVDATASGQKQ
>NZ_CP019365.1|WP_145954709.1|6518_7058_+|vsp-protein
MLDLATITKNITDAVAFAKDVKDVHTLVKSIDELAKAIKKKIQAGGLQDDTDNLNGTLLAGAYQIMADADSKLTALEGKSEKFAGIKDKVISAKQKSTAFLNKLKSENATLGAASAAVSSANAKEAIDRNNATKTKGAKELEELNTAIDTLLKAAEDAVTAAINALSTPAKSASTAQSN
>NZ_CP019365.1|WP_161491427.1|5412_6378_+|variable-large-family-protein
MSFFDSLIKIGQGFQEIFGIFGNAIGDTLGLTAVKSGDKRSKVGEHFKKIGDGLTTTKDKLDGLAKDIASAPHADITGVEITIKGASDVIVKLIDSVTKLAGVTNDSTDIGDARADANAGADGADKISVKAIIKEVKAIIETAEKSGVQIEKGTAGNSVENGNGPKVLAGGNARAAAGDAAKLADEVVKADPWAMINKIKDAKTKEGALSANANNDAGELATGTGAANVTAATNADLSSAVALKAMTKGGKFTQPAENEDGAVKAAAVTAVNKVLGILDFIIRKTVASELDKIREAVKGIKYSETTIEATETSTTQPTVTK
>NZ_CP019365.1|WP_088895136.1|4267_5257_+|variable-large-family-protein
MYCFYLVLLLNNNFYFLFLLLSCGSGQQAASSGSPGTAVSNSLIELGRSAENVFYSFMTLISDTLGFTAKSTTKKEDVGKYFTALGKKLGEASEELEQVAVKSTADVDKNDASKNSIRVAIDAAKTILITLKGYLDSLKGTGDANKVGEAATNAAGTAAADAELKAFFKALKGIVDTATTEGVAKPKAGDIAVKVGNGTDNKDGAKILGTDLAATAGDSSKAAILSTVSDEEMLASIVASKEGDAALVANATAQTSAISFAKGGNAAHLAGADAQGGQGEVQAVGVAAVNKLLVAVEDIVKKTVKNVLKTAKEKIDKARAPKSAGQQIR
>NZ_CP019365.1|WP_088895135.1|3417_4065_+|hypothetical-protein
MKRITLSALLMTLFLLMSCNNSGTSPKDGQAAKSDGTVIDLATISKNIKDTVAFAKSVKDVHTLVKSIDELAKAIGQKIQQNSDQFANDGAHNGSLISGAFQVILTVETKLKSLEDTVGLSDTLKTKVTSSKIASRAFLDKVKSKHTELGKEGASDADAKAAILVSNGTKDKGVDELVKLNTEIDALLTAAEAAVTAAINALSTPAKSDAPAQSN
>NZ_CP019365.1|WP_088895133.1|1530_2628_-|hypothetical-protein
MKSTKKTTNKYQHKLIVLISTLNYMNLNLKQYNQSNILYYFNNNMKKNDQKPVKLKTLQSYLYKLEKEFKVTINYHRHLGVNMGTEVYYKLKYSKKECYRIINKHFRDKKEERHENRVNAYLEKTCTKNSSVEKWECLYNKYNKKEEDKNNTKYIERLKVEKYAKKCNFKSNAFFSILNLEADKDFKIQALKAIKIAENSGYEKISDTKPNNTKLESKQKELSKILNETKANLKNEGYDSKQLESQIQNVYEQYKNKPHFIIERDKYSDLKKIIGKLKKTVEYTNKNTQKNERDIRNNVFSILLEQLRHKVDISVLVPILKNYLSRQNTLEYNKVFSNHYHYELLELMEDNKDYLQLGESKKVTS
>NZ_CP019365.1|WP_088895140.1|12446_13496_+|variable-large-family-protein
MKRITLSALLMTLFLLLSCGSGSAKAEDPKTLFLTSIANLGKGFLDVFTSLSDMITGAFGIKAETKKSEVGKYFTDIANTMTSVKEKLNTVVTAHGNYPKVKSVVDTFITNTLDKIADGAKTAASGATSSDAIGNAVKAGQDAAPAEVASVNALVKGIKDIVGVVLKDGEGDAGATKTEEAEKKSIGKLFSGKKDTDGTETQAAAASASIGAVTGADILKAIAKSALTEADKDIDKASNAAEIATAKADANGKDFGDGQKDAVIAAGIALRAMAKGGKFAAKTGEDKSAHAVNGAATSAVGKTLNTLIIAIRNTVDSGLKTINAVLATVTQEDKSAEATTPADATASGQ
>NZ_CP019365.1|WP_088895141.1|13562_14171_+|hypothetical-protein
MRIVFLLFISCNNSATAPKDGQAAKSDGTVIDLATITKNIKDTVAFAKSVKEVHTLVKSVDELAKAIGKKIKTDETGALEAFTADQNEQLIAGAFQVVSAVKGELESLVQVDGISDALKAKVNEAKNANDGLLVKFKSSAKDNESVKKDAEAKKVIDKTDASASDLKKLNTVIDELLTSAESAVTAAITQLTTSVKAEPTKS
>NZ_CP019365.1|WP_161491429.1|14483_14876_+|variable-large-family-protein
MANLGKGFLDVFTLLSDMITGAFGIKSETKKSDIDKYFTSIETTMNTVKAKLNDVVAKNGKFAANAEKDANAVNGVAANAIGKTLSTLIIAIRNTVDSGLKTISDALATVTQEDKSVDSTTPTDAATGGQ
>NZ_CP019365.1|WP_161491419.1|15295_15475_-|hypothetical-protein
MLRLLSTVSILVSKSLISDFNSFLTLANLLSRSLISACKVASTFSSLRLKVVSKYSISL
>NZ_CP019365.1|WP_088895143.1|16179_17268_+|variable-large-family-protein
MKHRLNERIKNFNITILISLFLLISCGSGQLQAEKLAAESKISFFDSLIKIGQGFQEIFGNAIGDILGFSAVKSTDSKSKSKVGEHLNKVKKGLEDTKTKLNDLSGKISEVTSADGNTIEAVKSVIKGTGEVFDKLIASVTKLAGVTNDGTAIGDTANADASAPANKDSVEAIIVGVKEIIDVADKSGIKIEAGNAGSPVANGAGTATATINANAAAGAGAGPKLSDEVAKADPWAMIAKIKDAKTKTEQLTAGADNNAGELATGANAADANGAKAATNADLAAAVALKAMTQGGKLSANAANNEADAVKAAAVSAVNKVLGVLDFIIRKTVSSQLEKIREAVKGIQYSETTAEASEAGTDK
>NZ_CP019365.1|WP_161491420.1|17581_17743_+|hypothetical-protein
MISLITGTLDKIAAGAKKAVSGDTNVASIADIVIVFSLQVSRTLLKEEEKLLL
>NZ_CP019365.1|WP_145954713.1|17913_18228_+|variable-large-family-protein
MVFVMMKTYYSKSKRYTTIASSIDLIAMAKDGKFAAKNGEDKSAHAVNGVAASAVGKTLSTLIIAIRNTVDIGLKTISDALVTVTQEDKSADSTTPTDVTASGQ
>NZ_CP019365.1|WP_161491421.1|18679_18841_-|hypothetical-protein
MLSRSLISDFNSFLTMSILLSSSVLTEIISDFKLRSNISNLRLKLLSKYSISL
>NZ_CP019365.1|WP_145954714.1|19445_20444_+|variable-large-family-protein
MTLFLLLSCGSGSTKAEDPQSRFLKSVISLGNDFLNVFTSLSDMVGGVLGFNTKKSDIGVYFKKVHDTLSSTKTALEKIVTDMKSENNPNTAATETAINKLVSETLDKIIEGASEAVKGAEGNDSIASVGTANVGAAGEENAVKSLIEGIGKIVEVVLGNKGSADAGDNNNAENGNARNNNGAGKLFANANAGAAAEAKKVAADAAKAVGAVTGADILQAMVKNDAVTLAKHAGGAGAESNKKDAIIAGGIALRAIAKNGKFSGASDGDADAKKAIEGVALSAVTKALNTLTIAIRNTIDVGLKGVKDAMKINPEDIPPVTNDNTTSETKKN
>NZ_CP019365.1|WP_088895147.1|20551_21163_+|hypothetical-protein
MTLFLLMSCNNSGTIPKDGHAAKSDGTLIDLATITKNIKDTVAFAKSVKDVHTLVMSIDELAKVIGKKIDANGLATESAHNGSLIAGAYSVIEVVDTKLGTLAGKVGLSSDLKAKVGSAKKESTAFLAKVKADHANLGKEDVDDAHAKNVIDVTDGTKDKGASELIKLNTVIDELLKAAEDAVTAAINALSIPAKSDSPTQSN

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Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity
NZ_CP019365_1	1.1\|12042\|30\|NZ_CP019365\|CRISPRCasFinder	12042-12071	30	NZ_CP019365	Borrelia turicatae 91E135 isolate Oz1 plasmid lpE43, complete sequence	12042-12071	0	1.0
NZ_CP019365_1	1.1\|12042\|30\|NZ_CP019365\|CRISPRCasFinder	12042-12071	30	NZ_CP019368	Borrelia turicatae 91E135 isolate Oz1 plasmid lpT39, complete sequence	4255-4284	0	1.0
NZ_CP019365_1	1.1\|12042\|30\|NZ_CP019365\|CRISPRCasFinder	12042-12071	30	NZ_CP015636	Borrelia turicatae strain BTE5EL plasmid lp48	1725-1754	2	0.933
NZ_CP019365_1	1.1\|12042\|30\|NZ_CP019365\|CRISPRCasFinder	12042-12071	30	NZ_CP015632	Borrelia turicatae strain BTE5EL plasmid lp30	21348-21377	5	0.833
NZ_CP019365_1	1.1\|12042\|30\|NZ_CP019365\|CRISPRCasFinder	12042-12071	30	NZ_CP015632	Borrelia turicatae strain BTE5EL plasmid lp30	21294-21323	6	0.8
NZ_CP019365_1	1.1\|12042\|30\|NZ_CP019365\|CRISPRCasFinder	12042-12071	30	NC_047956	Pseudomonas phage Achelous, complete genome	24812-24841	6	0.8

1. spacer 1.1|12042|30|NZ_CP019365|CRISPRCasFinder matches to NZ_CP019365 (Borrelia turicatae 91E135 isolate Oz1 plasmid lpE43, complete sequence) position: , mismatch: 0, identity: 1.0

ctgaaatcagacattaaggacctcgatact	CRISPR spacer
ctgaaatcagacattaaggacctcgatact	Protospacer
******************************

2. spacer 1.1|12042|30|NZ_CP019365|CRISPRCasFinder matches to NZ_CP019368 (Borrelia turicatae 91E135 isolate Oz1 plasmid lpT39, complete sequence) position: , mismatch: 0, identity: 1.0

ctgaaatcagacattaaggacctcgatact	CRISPR spacer
ctgaaatcagacattaaggacctcgatact	Protospacer
******************************

3. spacer 1.1|12042|30|NZ_CP019365|CRISPRCasFinder matches to NZ_CP015636 (Borrelia turicatae strain BTE5EL plasmid lp48) position: , mismatch: 2, identity: 0.933

ctgaaatcagacattaaggacctcgatact	CRISPR spacer
ctgaaatcagacattaaggacctcgattcc	Protospacer
*************************** *.

4. spacer 1.1|12042|30|NZ_CP019365|CRISPRCasFinder matches to NZ_CP015632 (Borrelia turicatae strain BTE5EL plasmid lp30) position: , mismatch: 5, identity: 0.833

ctgaaatcagacattaaggacctcgatact	CRISPR spacer
ttaaaatcagacattaaagacctggataat	Protospacer
.*.**************.***** **** *

5. spacer 1.1|12042|30|NZ_CP019365|CRISPRCasFinder matches to NZ_CP015632 (Borrelia turicatae strain BTE5EL plasmid lp30) position: , mismatch: 6, identity: 0.8

ctgaaatcagacattaaggacctcgatact	CRISPR spacer
ttaaaatcagacattaaagacctagataac	Protospacer
.*.**************.***** **** .

6. spacer 1.1|12042|30|NZ_CP019365|CRISPRCasFinder matches to NC_047956 (Pseudomonas phage Achelous, complete genome) position: , mismatch: 6, identity: 0.8

ctgaaatcagacattaaggacctcgatact	CRISPR spacer
tttacatcagacattaagcacctcgctatt	Protospacer
.* * ************* ****** **.*

Prophage detection

Region	Region Position	Protein_number	Hit_taxonomy	Key_proteins	Att_site	Prophage annotation

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

Overview of predicted results

Overview of the results

Cas Category Instructions

Results visualization

1. NZ_CP019368

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CRISPR-Cas detection and classification

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Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection

2. NC_008710

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CRISPR-Cas detection and classification

Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection

3. NZ_CP019365

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CRISPR-Cas detection and classification

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Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection