CRISPRimmunity

Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Self targeting spacer number	Target MGE spacer number	Prophage number	Anti-CRISPR protein number
NC_010172	Methylorubrum extorquens PA1, complete sequence	5 crisprs	csa3,DEDDh,WYL,cas3	1	2	2	0

1. NC_010172

CRISPR-Cas detection and classification

Crispr_ID: NC_010172_1

• CRISPR_ID: NC_010172_1
• CRISPR_location: 2134186-2134388
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 3 spacers
• Cas_protein_info: csa3

Consensus repeat of NC_010172_1

Consensus_repeat	Method
TGCCCTCCCGCGGGCGGGAGGTG	CRISPRCasFinder

spacers of NC_010172_1

>1.1|2134209|35|NC_010172|CRISPRCasFinder
ATTACGTCTCGTCACCTGTAATGGGCGAGCGGACT
>1.2|2134267|39|NC_010172|CRISPRCasFinder
CGGAGCGTGCGTCGCAGCCGATGAGGGGGTCGAGGGGGA
>1.3|2134329|37|NC_010172|CRISPRCasFinder
ACAATTCATCTAGGTATACATGGCGTTGTCGAAGGTT

CRISPR arrays and Neighbor proteins around NC_010172_1

CRISPR arrays

The CRISPR arrays of NC_010172_1

>merge|NC_010172|1|2134186-2134388|CRISPRCasFinder
TGCCCTCCCATGGGGAGGAGTGAATTACGTCTCGTCACCTGTAATGGGCGAGCGGACTTGCCCTCCCGAAGGCGGGAGGACCGGAGCGTGCGTCGCAGCCGATGAGGGGGTCGAGGGGGATGCCCTCCCGCGGGCGGGAGGTGACAATTCATCTAGGTATACATGGCGTTGTCGAAGGTTTGCCCTCCCGCGGACGGGAGGTG

>NC_010172|1|1|2134186-2134388|CRISPRCasFinder
TGCCCTCCCATGGGGAGGAGTGA	ATTACGTCTCGTCACCTGTAATGGGCGAGCGGACT
TGCCCTCCCGAAGGCGGGAGGAC	CGGAGCGTGCGTCGCAGCCGATGAGGGGGTCGAGGGGGA
TGCCCTCCCGCGGGCGGGAGGTG	ACAATTCATCTAGGTATACATGGCGTTGTCGAAGGTT
TGCCCTCCCGCGGACGGGAGGTG

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
NC_010172.1|WP_012253466.1|2138271_2139024_-|toll/interleukin-1-receptor-domain-containing-protein	unknown	unknown	gnl|CDD|379328
NC_010172.1|WP_012253456.1|2127610_2128036_+|arsenate-reductase-(glutaredoxin)	unknown	unknown	gnl|CDD|182200
NC_010172.1|WP_012253471.1|2142296_2143493_+|FIST-C-terminal-domain-containing-protein	unknown	unknown	gnl|CDD|225825
NC_010172.1|WP_012253453.1|2125934_2126681_-|arsenical-resistance-protein-ArsH	unknown	unknown	gnl|CDD|274260
NC_010172.1|WP_012253470.1|2141511_2142120_+|glutathione-S-transferase-family-protein	unknown	unknown	gnl|CDD|223698
NC_010172.1|WP_012253468.1|2139527_2140079_-|metallophosphoesterase	unknown	unknown	gnl|CDD|277336
NC_010172.1|WP_012253455.1|2127083_2127611_+|arsenate-reductase-ArsC	unknown	unknown	gnl|CDD|319973
NC_010172.1|WP_190274614.1|2143524_2144868_+|hybrid-sensor-histidine-kinase/response-regulator	unknown	unknown	gnl|CDD|223715
NC_010172.1|WP_012253461.1|2131540_2132896_+|NAD(P)-binding-protein	unknown	unknown	gnl|CDD|224983
NC_010172.1|WP_012253460.1|2131176_2131494_+|helix-turn-helix-transcriptional-regulator	unknown	unknown	gnl|CDD|378968
NC_010172.1|WP_012253458.1|2129399_2130284_-|hypothetical-protein	unknown	unknown	unknown
NC_010172.1|WP_012253465.1|2137891_2138248_+|hypothetical-protein	unknown	unknown	unknown
NC_010172.1|WP_012253457.1|2128070_2129366_+|arsenic-transporter	unknown	unknown	gnl|CDD|185342
NC_010172.1|WP_012253459.1|2130488_2131076_-|hypothetical-protein	unknown	unknown	unknown
NC_010172.1|WP_012253473.1|2144890_2145214_-|hypothetical-protein	unknown	unknown	unknown
NC_010172.1|WP_012253467.1|2139228_2139525_+|hypothetical-protein	unknown	unknown	unknown
NC_010172.1|WP_012253462.1|2132892_2134056_+|MFS-transporter	unknown	unknown	gnl|CDD|340913
NC_010172.1|WP_012253464.1|2136737_2137847_+|DNA-binding-protein	unknown	unknown	gnl|CDD|378704
NC_010172.1|WP_042509172.1|2126731_2127091_+|helix-turn-helix-transcriptional-regulator	csa3	COG0640_csa3_CAS-I-A	gnl|CDD|223713
NC_010172.1|WP_012253463.1|2134826_2136185_+|hypothetical-protein	unknown	unknown	unknown

HMMScan for Signature genes

Protein	Cas_name	Cas_description	E-value	Identity	Coverage
NC_010172.1|WP_042509172.1|2126731_2127091_+|helix-turn-helix-transcriptional-regulator	csa3	COG0640_csa3_CAS-I-A	2.3e-12	42.1	0.0

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
NC_010172.1|WP_012253466.1|2138271_2139024_-|toll/interleukin-1-receptor-domain-containing-protein	gnl|CDD|379328	pfam13676, TIR_2, TIR domain. This is a family of bacterial Toll-like receptors.	7.40663e-19
NC_010172.1|WP_012253456.1|2127610_2128036_+|arsenate-reductase-(glutaredoxin)	gnl|CDD|182200	PRK10026, PRK10026, arsenate reductase (glutaredoxin).	2.90369e-61
NC_010172.1|WP_012253471.1|2142296_2143493_+|FIST-C-terminal-domain-containing-protein	gnl|CDD|225825	COG3287, COG3287, Uncharacterized conserved protein [Function unknown].	1.25993e-80
NC_010172.1|WP_012253453.1|2125934_2126681_-|arsenical-resistance-protein-ArsH	gnl|CDD|274260	TIGR02690, arsenical_resistance_protein_ArsH_putative, arsenical resistance protein ArsH. Members of this protein family occur in arsenate resistance operons that include at least two different types of arsenate reductase. ArsH is not required for arsenate resistance in some systems. This family belongs to the larger family of NADPH-dependent FMN reductases (pfam03358). The function of ArsH is not known. [Cellular processes, Detoxification].	1.0005e-121
NC_010172.1|WP_012253455.1|2127083_2127611_+|arsenate-reductase-ArsC	gnl|CDD|319973	cd16345, LMWP_ArsC, Arsenate reductase of the LMWP family. Arsenate reductase plays an important role in the reduction of intracellular arsenate to arsenite, an important step in arsenic detoxification. The reduction involves three different thiolate nucleophiles. In arsenate reductases of the LMWP family, reduction can be coupled with thioredoxin (Trx)/thioredoxin reductase (TrxR) or glutathione (GSH)/glutaredoxin (Grx).	5.20993e-67
NC_010172.1|WP_012253468.1|2139527_2140079_-|metallophosphoesterase	gnl|CDD|277336	cd07390, MPP_AQ1575, Aquifex aeolicus AQ1575 and related proteins, metallophosphatase domain. This family includes bacterial and archeal proteins homologous to AQ1575, an uncharacterized Aquifex aeolicus protein. AQ1575 may play an accessory role in DNA repair, based on the close proximity of its gene to Holliday junction resolvasome genes. The domain present in members of this family belongs to the metallophosphatase (MPP) superfamily. MPPs are functionally diverse, but all share a conserved domain with an active site consisting of two metal ions (usually manganese, iron, or zinc) coordinated with octahedral geometry by a cage of histidine, aspartate, and asparagine residues. The MPP superfamily includes: Mre11/SbcD-like exonucleases, Dbr1-like RNA lariat debranching enzymes, YfcE-like phosphodiesterases, purple acid phosphatases (PAPs), YbbF-like UDP-2,3-diacylglucosamine hydrolases, and acid sphingomyelinases (ASMases). The conserved domain is a double beta-sheet sandwich with a di-metal active site made up of residues located at the C-terminal side of the sheets. This domain is thought to allow for productive metal coordination.	2.15349e-37
NC_010172.1|WP_190274614.1|2143524_2144868_+|hybrid-sensor-histidine-kinase/response-regulator	gnl|CDD|223715	COG0642, BaeS, Signal transduction histidine kinase [Signal transduction mechanisms].	1.79843e-45
NC_010172.1|WP_012253461.1|2131540_2132896_+|NAD(P)-binding-protein	gnl|CDD|224983	COG2072, TrkA, Predicted flavoprotein involved in K+ transport [Inorganic ion transport and metabolism].	1.09078e-08
NC_010172.1|WP_012253460.1|2131176_2131494_+|helix-turn-helix-transcriptional-regulator	gnl|CDD|378968	pfam12840, HTH_20, Helix-turn-helix domain. This domain represents a DNA-binding Helix-turn-helix domain found in transcriptional regulatory proteins.	3.28914e-09
NC_010172.1|WP_012253457.1|2128070_2129366_+|arsenic-transporter	gnl|CDD|185342	PRK15445, PRK15445, arsenical efflux pump membrane protein ArsB.	0
NC_010172.1|WP_012253470.1|2141511_2142120_+|glutathione-S-transferase-family-protein	gnl|CDD|223698	COG0625, Gst, Glutathione S-transferase [Posttranslational modification, protein turnover, chaperones].	7.37762e-37
NC_010172.1|WP_012253464.1|2136737_2137847_+|DNA-binding-protein	gnl|CDD|378704	pfam11740, KfrA_N, Plasmid replication region DNA-binding N-term. The broad host-range plasmid RK2 is able to replicate in and be inherited in a stable manner in diverse Gram-negative bacterial species. It encodes a number of co-ordinately regulated operons including a central control korF1 operon that represses the kfrA operon. The KfrA polypeptide is a site-specific DNA-binding protein whose operator overlaps the kfrA promoter. The N-terminus, containing an helix-turn-helix motif, is essential for function. Downstream from this family is an extended coiled-coil domain containing a heptad repeat segment which is probably responsible for formation of multimers, and may provide an example of a bridge to host structures required for plasmid partitioning.	4.0941e-10
NC_010172.1|WP_042509172.1|2126731_2127091_+|helix-turn-helix-transcriptional-regulator	gnl|CDD|223713	COG0640, ArsR, Predicted transcriptional regulators [Transcription].	4.95682e-15
NC_010172.1|WP_012253462.1|2132892_2134056_+|MFS-transporter	gnl|CDD|340913	cd17355, MFS_YcxA_like, MFS-type transporter YcxA and similar proteins of the Major Facilitator Superfamily of transporters. This group is composed of uncharacterized bacterial MFS-type transporters including Bacillus subtilis YcxA and YbfB. YcxA has been shown to facilitate the export of surfactin in B. subtilis. The YcxA-like group belongs to the Monocarboxylate transporter -like (MCT-like) family of the Major Facilitator Superfamily (MFS) of membrane transport proteins. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.	3.45488e-38

Protein sequences

>NC_010172.1|WP_012253462.1|2132892_2134056_+|MFS-transporter
MSGRGRLTVISALGVVEILAWGSSFYLPAVLAGPIAADTGWPLAWVVGGLSIGLLVAAVASPRVGIAIQRHGGRPVLALAAVLLAVGLAALGLAPNLPAFLAGWLVVGLGMGCGLYDPAFATLGRLYGSEARPAITTLTLWGGFASTVCWPLSAFLVEQVGWRHACLAYAGLHLLVTLPLVLGLIPRAPAAEAARGEVHHRGGMLTARERRAFLLMAGVLVLGGAVMTLVSVHLITLLQARGVALAAAVSYGALIGPAQVGARIVEMAGKGRHHPLWTLTAAMVLVAAGLAVLAAGIPAVGLALVLYGAGNGIYSIARGTVPLSLFGPERYATLVGRLARPGLAAQALAPSLGAAALAYGGADTAYVLLLALALANVVLVAALWGAR
>NC_010172.1|WP_012253461.1|2131540_2132896_+|NAD(P)-binding-protein
MPERSSLPVAVIGAGPVGLAAAAHLVARGLPVRVYEAGSEVGAHVRAWGHVRLFSPWRYNIDPAARALLEAEGWIAPSDDGFPTGHELADRYLAPLAATPELAPVVETGARVTAVGRLGMDKAGSRDRAARPFVLTVTDPGGGIRRDLARAVIDASGTWGQPNPLGAGGLPAEGEADLADRIVTGIPDVLGRERAAYAGRTTLVVGAGHSAANVLLDLARLADGVEGTKAVWATRGTDLSRVYGGGEADELAGRGDLGTRLRGLIEGGRLPLVRGFAASSVRECCGRLLVDGTVAGEPRTLGPVDRIVVATGQRPDLDLTREVRLDLDPWLESVRALGPLIDPNLHSCGSVQPHGHREVSHPEPGFYTVGIKSYGRAPTFLMLTGYEQVRSVADALAGDLAAADDVRLTLPQTGVCSTSRASHAGSGGCCGPVPADAQTADAAGCGCGVPA
>NC_010172.1|WP_012253460.1|2131176_2131494_+|helix-turn-helix-transcriptional-regulator
MNVEQAAKQLESLGSPARLRIYRALVRAGHAGLPVRQLQERVGIPAASTLSHHLHRLILTGLVTQERQATTLICRAVYPAMNALVGYLSDECCADEACSDKGEAA
>NC_010172.1|WP_012253459.1|2130488_2131076_-|hypothetical-protein
MTHDDLLDALRSHDALLIHCSPSGKGQKPDDLVYPDDLRKAIQVLDEGRGALCCSVVWPQHTAAFGTVGIVIKPRSAASVLGVVTGDGGTTYDPQTRIRTSGRTVPLTIQNLPLTFTPTTEHNEWLVEDADCIGIYFVAGEYAQVAERVGPPGPDGRYDTQIRNLTMADVKRDFPGLPIFCLMGNGVVGFMGNPY
>NC_010172.1|WP_012253458.1|2129399_2130284_-|hypothetical-protein
MIYVVDQNMMRGPRLHEIIRTEPDSAFVIPDVAFEEMSRLEKWEDTMRGSFLAFAPILDRTYCSAPCGELIDFEAQNRSPVPRERLLPAEITDFGRSFIEAVVTGSERLGAFREMMVGTRQDMLAARPDGASLKEKLQQAVSQLRGLGDPDLDKDLRAGRIDAPAQLGLIKVRADALVRQFLGFDLPDVGTPRMSNLFTARFTYAHLWYRQRWLRDSGLDNVDLKKVTNDDYDQDYALIASYFDGLLAKDEKCKACAEDLRSLTQERSEEEAMDALDAYAVSSGRIPPAATDMP
>NC_010172.1|WP_012253457.1|2128070_2129366_+|arsenic-transporter
MLALAIFLVTLVFVIWQPRGLGIGWSALAGAGVALATGVIHPGDIPVVWHIVWDATFTFVALIIISLLLDEAGFFHWAALHIARWGGGRGRRLFPLVILLGAAIAAVFANDGAALLLTPIVLAVLLRLDFKPAAALAFIVACGFVADSTSLPLVISNLVNIVSANFFDVTFGRYAAVMVPVDLVSLAATLLVLWAYFRRDVPATYPVDALERPAEAIRDPLVFRAAFPLLGVLLLAYFVTAPFGVPVSVVTCAGAAVLLLLANRGGTIPIRKVLTGAPWQIVLFSLGMYLVVYGLRNAGLTDELAKGLVWLAGHGPWVATVGTGFAAAILSSVMNNMPSVLIGALSIQQAPDLSPLTRELMVYANVIGCDLGPKFTPIGSLATLLWLHVLDSKGQRITWGQYMKVGLVITPPVLLVTLLALAVWLPVLGPQ
>NC_010172.1|WP_012253456.1|2127610_2128036_+|arsenate-reductase-(glutaredoxin)
MDVVIYHNPDCGTSRNTLAMIRNAGIEPHVVEYLKTPPARPLLVRMLARAGLSVRDVLREKGTPFAELNLGDPALTDAQLLDAIEAHPILINRPLVVSPKGVRLCRPSEAVLDLLPGQQGEFVKEDGERVVDEHGRRVATA
>NC_010172.1|WP_012253455.1|2127083_2127611_+|arsenate-reductase-ArsC
MPEPVYNVLFLCTGNSARSILAEAMLNKEGAGRFRAFSAGSQPKGEPHPLALQVLRESDHPTEGLRSKSWGEFAGPDAPVMDFVFTVCDNAAGEACPFWPGQPVTAHWGIEDPAAAEGSEMERKRAFVTAQRYLKNRISALVALPLGSLDQVALSSKVREIGQLAGATSARPEVA
>NC_010172.1|WP_042509172.1|2126731_2127091_+|helix-turn-helix-transcriptional-regulator
MDETQALAAFLALGQEHRLRLVRALVTAGPGGLASGVLADAVGVSAATVSHHLKELSHAGLVTSRREGRSIIYSAAYPALSDLVQFLMRDCCQGHPEVCAPALAALTACDCTTGDTLHA
>NC_010172.1|WP_012253453.1|2125934_2126681_-|arsenical-resistance-protein-ArsH
MDKPAQPFTDGLPNLSEAHFEAPNAERVSAAVPLAHAPRFLILYGSLRERSFSRFLAYEAARLLEAMGGDVRIYDAHGLPLPDDSTADHPKVQELRQLSIWSEGQVWVSPERHGNMTGVMKAQVDWLPLSEGSVRPTQGRTLAVMQVSGGSQSFNAVNSLRVLGRWMRMITIPNQSSVPMAYKEFDDAGRMKPGPLYDRVVDVCEELMKFTLLTRGRADYLVDRYSERKEREPKRLMAVAADIGFVKR
>NC_010172.1|WP_012253463.1|2134826_2136185_+|hypothetical-protein
MHPDPGFPHPAADVDPPGADARGLAKARQTISSLAHPHTRPIVDTAMGTLHAALSGGGLQVSKIPGLQIAVRKHVRQAWTDGIIAPLARSRHPAAREVLDAMPKDFAMKAHAEVLAVVERISRGVPEDAVATREHVARAIEYLETLPLILRALVDVEGVASGARTGRPAEATGPADLPGAIALAQRRAQAALSPHLSSLVEGLADLVYLGPLGATGNPAFRWTAAALLAPPARGARKSDRGGPQWRRGLANALSRDLVDEALAPDAGRLLRRHAGLEVGDASCTRGEGHAGRLDLALSRAGVRLVLSVGFDAASGRFERRAEAASFDGVRTPLALEDADALLDASVPGSLDTPAHRAMRRLARQDDALALHIHVNATGELQVAPGFLRDGDAGLLEGLVRAADEEGSAVRFDPVRDLVLASGLVEFDFEWHDGSAGRCLVRTPVGPARRPGA
>NC_010172.1|WP_012253464.1|2136737_2137847_+|DNA-binding-protein
MVAVNPVWQAADRIRARPAGQDGRKERVSVRSVRKELGKGSFSDIARELKVWREREDYHPVIEQADLPEAFERRLTVLGRELLEMARVEVARATLADFAGSERRRSAERDLLDEAFDRVDFLEERVAALQAELDRRPAGTGRGQAAPAQGAPAAEPEREWKGSPFADMAQGISLAKRADVFWEQVRIAVEDVFRRRGPLAVHPLFKSLPKQLKQDGEQVGFPLTEGWLRYHLLHLVQGGGLALDGYRFGLAEPAPGPDDGPAVDASAADDAAAMGRRRFWRQYMLDVHDLLVKEGPMTVAGIIDGMGPGWVEASERYRKITVGRIRYKLRGRIVEGRPFVELADGRFAAVPVEGHWDGLSAARTAEGNG
>NC_010172.1|WP_012253465.1|2137891_2138248_+|hypothetical-protein
MTPQEINAKVRGAYVRALQSSTMYSMSGSRIDDLFRDVKLYGRDAGTDFAETNLGRIVEEAARMAGCKRPCLEIQVYGWGRAALDGLAQTLRDRTGLKVDVAGPTVRLDWSEDKAGPV
>NC_010172.1|WP_012253466.1|2138271_2139024_-|toll/interleukin-1-receptor-domain-containing-protein
MRARARTHTEKPGPTSKTAAKAKPGPRSPARPAVPRPKPEKPEVRIFVSYSHRDADARQRLETHLAPLKREGVTTWFDGDMNAGDKLDTGIARALRNAHVFVGLLSPDYLASNYCWKIEYKRAMGRRANGTMRVVAVVLRPCDWKSTTAAGFKLLPEDGKEVTRWRSADDAFLNVAQGIRRVVADVRKTMAAAPPKPAPRKPAASKAAAQPPTKPGPKPRQKPGPKPNPGRAGAGAVARKRKPPAKGRKP
>NC_010172.1|WP_012253467.1|2139228_2139525_+|hypothetical-protein
MRMETAREALAQGRCLEVRYDDYARVVEVHACGFTGQGHAVMRVWQVRGGSVSGERTGWKLLRLDEALALAVTGEASEAPRRGYRRGDAGMARIVAQV
>NC_010172.1|WP_012253468.1|2139527_2140079_-|metallophosphoesterase
MPNTFFTSDSHFGHASVLAPRMDRPRPFATIAEHDEALIAAWNAVVRPGDIVWHLGDFAYRCDLAYAASIKARLNGRIRLIRGNHEELGGQLDWDKPVVDVQRVFVQDPGMPKPQAIWCSHYAHRTWPHERHGDLHLYGHSHGSLPGTRTSLDVGVDCWDWAPVRLDEILKRMAATPEPVPAA
>NC_010172.1|WP_012253470.1|2141511_2142120_+|glutathione-S-transferase-family-protein
MITVFHAPRTRSLRVLWMLEEMGLPYEVRLVDFPRHRSDAEFMRLNPAGSIPVLRDGDVVISESTAIIEYLIARYGPTPLAPQPSEAAYPSYLQYFHFGEASLAGPMTVVAHCWFMGPEDQRENAGVEAARTIFRARLPAIQSRLEEHDYLAGSAFTAADVSVVYALGLGQALRAVETYAPVIQEYLARCRARPGFQRASMK
>NC_010172.1|WP_012253471.1|2142296_2143493_+|FIST-C-terminal-domain-containing-protein
MAHGTQMRGHLCGIQTAWTDAVDALGAVAAVAGAIDRSVVGHLIVFFSPAYDAEILTAALAAQFPGVGLTGCSTSGGISPAGSIDRGLVAIAFPRQGFRIVSGLLTDIARLDVEGAASTVRGLRRTLDGGRPDGGSGHRFALSLIDSLANAEETVVSAVAWALDGIPLIGGSAGDDLAFRSTVLIHEGQVCREAAVILLVETDFPIRIFKSDNFEPTNRKFVVTAAREDERRVTELNAEPAAREYAMAVGLDPENLSTMSFAAYPLAVKIGGEYYCRSISRVEPDGSMTFFCAIGEGVVLTLAQPRDIVEATRAELESLDASLGGLDLVIGFDCVFRRLDAESRQVRHRIADLYRRYGVVGFETYGEQYRSMHLNQTFTGIAIGRAEAPERRDAKIAT
>NC_010172.1|WP_190274614.1|2143524_2144868_+|hybrid-sensor-histidine-kinase/response-regulator
MEPPAEPQTIEALTHRVAKLERINAALMAHVERTMDQQGGAYSLFQTAIMLEGRVRARTEELTALMNSLERSNAAMQAAKEEAETANRSKTRFLAAASHDLLQPLNAARLSLSALTDLAPGPEAQGIARQVERGLETIEDLIKALIDISKLDAGIVRPVIKPVRLADLVAGIEASFRPFAERKELRLVTRCADLVVETDGILLQRILQNLVSNAIRYTESGGVLIAARRRDKLCRIDVVDTGCGIPEAERALVFEEFFRGARECDDGGIGLGLGLSIVQRMASTLGHAIELHSRSGHGTRLSLTLPLATQRPDPRVTLAPLATALTGARVLAIENDASTAEALQRLLRNWDAEVQVFRDLAGVVAALGAGLARPDVMVIDYHLDNGACGLDVVDYLRRNRGWVTPVILTTADHGADIAARAQATGAELVHKPIKPAQLRSLLAYMLA
>NC_010172.1|WP_012253473.1|2144890_2145214_-|hypothetical-protein
MRRVLLASLTTLAVLAALPARAESPEGARHAAWQVCLDEAFAEQILTTSRSFAATKAVSTCRDREEAYLGVLAGSPLLDGEDVTRIRPALVARARDRLMGERRFSAL

Crispr_ID: NC_010172_2

• CRISPR_ID: NC_010172_2
• CRISPR_location: 2136420-2136510
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 1 spacers
• Cas_protein_info: csa3

Consensus repeat of NC_010172_2

Consensus_repeat	Method
GATGGCGACGATACCCTGCCAAGCGA	CRISPRCasFinder

spacers of NC_010172_2

>2.1|2136446|39|NC_010172|CRISPRCasFinder
CGGGTCTGCCCTCCCGTGGGCGGGAGGTGGGTTGAACCA

CRISPR arrays and Neighbor proteins around NC_010172_2

CRISPR arrays

The CRISPR arrays of NC_010172_2

>merge|NC_010172|2|2136420-2136510|CRISPRCasFinder
GATGGCGACGATACCCTGCCAAGCGACGGGTCTGCCCTCCCGTGGGCGGGAGGTGGGTTGAACCAGATGGCGACGATACCCTGCCAAGCGA

>NC_010172|2|2|2136420-2136510|CRISPRCasFinder
GATGGCGACGATACCCTGCCAAGCGA	CGGGTCTGCCCTCCCGTGGGCGGGAGGTGGGTTGAACCA
GATGGCGACGATACCCTGCCAAGCGA

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
NC_010172.1|WP_012253474.1|2145526_2146195_-|transglutaminase-like-cysteine-peptidase	unknown	unknown	gnl|CDD|368714
NC_010172.1|WP_012253466.1|2138271_2139024_-|toll/interleukin-1-receptor-domain-containing-protein	unknown	unknown	gnl|CDD|379328
NC_010172.1|WP_012253456.1|2127610_2128036_+|arsenate-reductase-(glutaredoxin)	unknown	unknown	gnl|CDD|182200
NC_010172.1|WP_012253471.1|2142296_2143493_+|FIST-C-terminal-domain-containing-protein	unknown	unknown	gnl|CDD|225825
NC_010172.1|WP_012253470.1|2141511_2142120_+|glutathione-S-transferase-family-protein	unknown	unknown	gnl|CDD|223698
NC_010172.1|WP_012253468.1|2139527_2140079_-|metallophosphoesterase	unknown	unknown	gnl|CDD|277336
NC_010172.1|WP_012253455.1|2127083_2127611_+|arsenate-reductase-ArsC	unknown	unknown	gnl|CDD|319973
NC_010172.1|WP_190274614.1|2143524_2144868_+|hybrid-sensor-histidine-kinase/response-regulator	unknown	unknown	gnl|CDD|223715
NC_010172.1|WP_012253461.1|2131540_2132896_+|NAD(P)-binding-protein	unknown	unknown	gnl|CDD|224983
NC_010172.1|WP_012253460.1|2131176_2131494_+|helix-turn-helix-transcriptional-regulator	unknown	unknown	gnl|CDD|378968
NC_010172.1|WP_012253458.1|2129399_2130284_-|hypothetical-protein	unknown	unknown	unknown
NC_010172.1|WP_012253465.1|2137891_2138248_+|hypothetical-protein	unknown	unknown	unknown
NC_010172.1|WP_012253457.1|2128070_2129366_+|arsenic-transporter	unknown	unknown	gnl|CDD|185342
NC_010172.1|WP_012253459.1|2130488_2131076_-|hypothetical-protein	unknown	unknown	unknown
NC_010172.1|WP_012253473.1|2144890_2145214_-|hypothetical-protein	unknown	unknown	unknown
NC_010172.1|WP_012253467.1|2139228_2139525_+|hypothetical-protein	unknown	unknown	unknown
NC_010172.1|WP_012253462.1|2132892_2134056_+|MFS-transporter	unknown	unknown	gnl|CDD|340913
NC_010172.1|WP_012253464.1|2136737_2137847_+|DNA-binding-protein	unknown	unknown	gnl|CDD|378704
NC_010172.1|WP_042509172.1|2126731_2127091_+|helix-turn-helix-transcriptional-regulator	csa3	COG0640_csa3_CAS-I-A	gnl|CDD|223713
NC_010172.1|WP_012253463.1|2134826_2136185_+|hypothetical-protein	unknown	unknown	unknown

HMMScan for Signature genes

Protein	Cas_name	Cas_description	E-value	Identity	Coverage
NC_010172.1|WP_042509172.1|2126731_2127091_+|helix-turn-helix-transcriptional-regulator	csa3	COG0640_csa3_CAS-I-A	2.3e-12	42.1	0.0

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
NC_010172.1|WP_012253474.1|2145526_2146195_-|transglutaminase-like-cysteine-peptidase	gnl|CDD|368714	pfam06035, Peptidase_C93, Bacterial transglutaminase-like cysteine proteinase BTLCP. Members of this family are predicted to be bacterial transglutaminase-like cysteine proteinases. They contain a conserved Cys-His-Asp catalytic triad. Their structure is predicted to be similar to that of Salmonella typhimurium N-hydroxyarylamine O-acetyltransferase, in pfam00797, however they lack the sub-domain which is important for arylamine recognition.	1.31821e-87
NC_010172.1|WP_012253466.1|2138271_2139024_-|toll/interleukin-1-receptor-domain-containing-protein	gnl|CDD|379328	pfam13676, TIR_2, TIR domain. This is a family of bacterial Toll-like receptors.	7.40663e-19
NC_010172.1|WP_012253456.1|2127610_2128036_+|arsenate-reductase-(glutaredoxin)	gnl|CDD|182200	PRK10026, PRK10026, arsenate reductase (glutaredoxin).	2.90369e-61
NC_010172.1|WP_012253471.1|2142296_2143493_+|FIST-C-terminal-domain-containing-protein	gnl|CDD|225825	COG3287, COG3287, Uncharacterized conserved protein [Function unknown].	1.25993e-80
NC_010172.1|WP_012253455.1|2127083_2127611_+|arsenate-reductase-ArsC	gnl|CDD|319973	cd16345, LMWP_ArsC, Arsenate reductase of the LMWP family. Arsenate reductase plays an important role in the reduction of intracellular arsenate to arsenite, an important step in arsenic detoxification. The reduction involves three different thiolate nucleophiles. In arsenate reductases of the LMWP family, reduction can be coupled with thioredoxin (Trx)/thioredoxin reductase (TrxR) or glutathione (GSH)/glutaredoxin (Grx).	5.20993e-67
NC_010172.1|WP_012253468.1|2139527_2140079_-|metallophosphoesterase	gnl|CDD|277336	cd07390, MPP_AQ1575, Aquifex aeolicus AQ1575 and related proteins, metallophosphatase domain. This family includes bacterial and archeal proteins homologous to AQ1575, an uncharacterized Aquifex aeolicus protein. AQ1575 may play an accessory role in DNA repair, based on the close proximity of its gene to Holliday junction resolvasome genes. The domain present in members of this family belongs to the metallophosphatase (MPP) superfamily. MPPs are functionally diverse, but all share a conserved domain with an active site consisting of two metal ions (usually manganese, iron, or zinc) coordinated with octahedral geometry by a cage of histidine, aspartate, and asparagine residues. The MPP superfamily includes: Mre11/SbcD-like exonucleases, Dbr1-like RNA lariat debranching enzymes, YfcE-like phosphodiesterases, purple acid phosphatases (PAPs), YbbF-like UDP-2,3-diacylglucosamine hydrolases, and acid sphingomyelinases (ASMases). The conserved domain is a double beta-sheet sandwich with a di-metal active site made up of residues located at the C-terminal side of the sheets. This domain is thought to allow for productive metal coordination.	2.15349e-37
NC_010172.1|WP_190274614.1|2143524_2144868_+|hybrid-sensor-histidine-kinase/response-regulator	gnl|CDD|223715	COG0642, BaeS, Signal transduction histidine kinase [Signal transduction mechanisms].	1.79843e-45
NC_010172.1|WP_012253461.1|2131540_2132896_+|NAD(P)-binding-protein	gnl|CDD|224983	COG2072, TrkA, Predicted flavoprotein involved in K+ transport [Inorganic ion transport and metabolism].	1.09078e-08
NC_010172.1|WP_012253460.1|2131176_2131494_+|helix-turn-helix-transcriptional-regulator	gnl|CDD|378968	pfam12840, HTH_20, Helix-turn-helix domain. This domain represents a DNA-binding Helix-turn-helix domain found in transcriptional regulatory proteins.	3.28914e-09
NC_010172.1|WP_012253457.1|2128070_2129366_+|arsenic-transporter	gnl|CDD|185342	PRK15445, PRK15445, arsenical efflux pump membrane protein ArsB.	0
NC_010172.1|WP_012253470.1|2141511_2142120_+|glutathione-S-transferase-family-protein	gnl|CDD|223698	COG0625, Gst, Glutathione S-transferase [Posttranslational modification, protein turnover, chaperones].	7.37762e-37
NC_010172.1|WP_012253464.1|2136737_2137847_+|DNA-binding-protein	gnl|CDD|378704	pfam11740, KfrA_N, Plasmid replication region DNA-binding N-term. The broad host-range plasmid RK2 is able to replicate in and be inherited in a stable manner in diverse Gram-negative bacterial species. It encodes a number of co-ordinately regulated operons including a central control korF1 operon that represses the kfrA operon. The KfrA polypeptide is a site-specific DNA-binding protein whose operator overlaps the kfrA promoter. The N-terminus, containing an helix-turn-helix motif, is essential for function. Downstream from this family is an extended coiled-coil domain containing a heptad repeat segment which is probably responsible for formation of multimers, and may provide an example of a bridge to host structures required for plasmid partitioning.	4.0941e-10
NC_010172.1|WP_042509172.1|2126731_2127091_+|helix-turn-helix-transcriptional-regulator	gnl|CDD|223713	COG0640, ArsR, Predicted transcriptional regulators [Transcription].	4.95682e-15
NC_010172.1|WP_012253462.1|2132892_2134056_+|MFS-transporter	gnl|CDD|340913	cd17355, MFS_YcxA_like, MFS-type transporter YcxA and similar proteins of the Major Facilitator Superfamily of transporters. This group is composed of uncharacterized bacterial MFS-type transporters including Bacillus subtilis YcxA and YbfB. YcxA has been shown to facilitate the export of surfactin in B. subtilis. The YcxA-like group belongs to the Monocarboxylate transporter -like (MCT-like) family of the Major Facilitator Superfamily (MFS) of membrane transport proteins. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.	3.45488e-38

Protein sequences

>NC_010172.1|WP_012253463.1|2134826_2136185_+|hypothetical-protein
MHPDPGFPHPAADVDPPGADARGLAKARQTISSLAHPHTRPIVDTAMGTLHAALSGGGLQVSKIPGLQIAVRKHVRQAWTDGIIAPLARSRHPAAREVLDAMPKDFAMKAHAEVLAVVERISRGVPEDAVATREHVARAIEYLETLPLILRALVDVEGVASGARTGRPAEATGPADLPGAIALAQRRAQAALSPHLSSLVEGLADLVYLGPLGATGNPAFRWTAAALLAPPARGARKSDRGGPQWRRGLANALSRDLVDEALAPDAGRLLRRHAGLEVGDASCTRGEGHAGRLDLALSRAGVRLVLSVGFDAASGRFERRAEAASFDGVRTPLALEDADALLDASVPGSLDTPAHRAMRRLARQDDALALHIHVNATGELQVAPGFLRDGDAGLLEGLVRAADEEGSAVRFDPVRDLVLASGLVEFDFEWHDGSAGRCLVRTPVGPARRPGA
>NC_010172.1|WP_012253462.1|2132892_2134056_+|MFS-transporter
MSGRGRLTVISALGVVEILAWGSSFYLPAVLAGPIAADTGWPLAWVVGGLSIGLLVAAVASPRVGIAIQRHGGRPVLALAAVLLAVGLAALGLAPNLPAFLAGWLVVGLGMGCGLYDPAFATLGRLYGSEARPAITTLTLWGGFASTVCWPLSAFLVEQVGWRHACLAYAGLHLLVTLPLVLGLIPRAPAAEAARGEVHHRGGMLTARERRAFLLMAGVLVLGGAVMTLVSVHLITLLQARGVALAAAVSYGALIGPAQVGARIVEMAGKGRHHPLWTLTAAMVLVAAGLAVLAAGIPAVGLALVLYGAGNGIYSIARGTVPLSLFGPERYATLVGRLARPGLAAQALAPSLGAAALAYGGADTAYVLLLALALANVVLVAALWGAR
>NC_010172.1|WP_012253461.1|2131540_2132896_+|NAD(P)-binding-protein
MPERSSLPVAVIGAGPVGLAAAAHLVARGLPVRVYEAGSEVGAHVRAWGHVRLFSPWRYNIDPAARALLEAEGWIAPSDDGFPTGHELADRYLAPLAATPELAPVVETGARVTAVGRLGMDKAGSRDRAARPFVLTVTDPGGGIRRDLARAVIDASGTWGQPNPLGAGGLPAEGEADLADRIVTGIPDVLGRERAAYAGRTTLVVGAGHSAANVLLDLARLADGVEGTKAVWATRGTDLSRVYGGGEADELAGRGDLGTRLRGLIEGGRLPLVRGFAASSVRECCGRLLVDGTVAGEPRTLGPVDRIVVATGQRPDLDLTREVRLDLDPWLESVRALGPLIDPNLHSCGSVQPHGHREVSHPEPGFYTVGIKSYGRAPTFLMLTGYEQVRSVADALAGDLAAADDVRLTLPQTGVCSTSRASHAGSGGCCGPVPADAQTADAAGCGCGVPA
>NC_010172.1|WP_012253460.1|2131176_2131494_+|helix-turn-helix-transcriptional-regulator
MNVEQAAKQLESLGSPARLRIYRALVRAGHAGLPVRQLQERVGIPAASTLSHHLHRLILTGLVTQERQATTLICRAVYPAMNALVGYLSDECCADEACSDKGEAA
>NC_010172.1|WP_012253459.1|2130488_2131076_-|hypothetical-protein
MTHDDLLDALRSHDALLIHCSPSGKGQKPDDLVYPDDLRKAIQVLDEGRGALCCSVVWPQHTAAFGTVGIVIKPRSAASVLGVVTGDGGTTYDPQTRIRTSGRTVPLTIQNLPLTFTPTTEHNEWLVEDADCIGIYFVAGEYAQVAERVGPPGPDGRYDTQIRNLTMADVKRDFPGLPIFCLMGNGVVGFMGNPY
>NC_010172.1|WP_012253458.1|2129399_2130284_-|hypothetical-protein
MIYVVDQNMMRGPRLHEIIRTEPDSAFVIPDVAFEEMSRLEKWEDTMRGSFLAFAPILDRTYCSAPCGELIDFEAQNRSPVPRERLLPAEITDFGRSFIEAVVTGSERLGAFREMMVGTRQDMLAARPDGASLKEKLQQAVSQLRGLGDPDLDKDLRAGRIDAPAQLGLIKVRADALVRQFLGFDLPDVGTPRMSNLFTARFTYAHLWYRQRWLRDSGLDNVDLKKVTNDDYDQDYALIASYFDGLLAKDEKCKACAEDLRSLTQERSEEEAMDALDAYAVSSGRIPPAATDMP
>NC_010172.1|WP_012253457.1|2128070_2129366_+|arsenic-transporter
MLALAIFLVTLVFVIWQPRGLGIGWSALAGAGVALATGVIHPGDIPVVWHIVWDATFTFVALIIISLLLDEAGFFHWAALHIARWGGGRGRRLFPLVILLGAAIAAVFANDGAALLLTPIVLAVLLRLDFKPAAALAFIVACGFVADSTSLPLVISNLVNIVSANFFDVTFGRYAAVMVPVDLVSLAATLLVLWAYFRRDVPATYPVDALERPAEAIRDPLVFRAAFPLLGVLLLAYFVTAPFGVPVSVVTCAGAAVLLLLANRGGTIPIRKVLTGAPWQIVLFSLGMYLVVYGLRNAGLTDELAKGLVWLAGHGPWVATVGTGFAAAILSSVMNNMPSVLIGALSIQQAPDLSPLTRELMVYANVIGCDLGPKFTPIGSLATLLWLHVLDSKGQRITWGQYMKVGLVITPPVLLVTLLALAVWLPVLGPQ
>NC_010172.1|WP_012253456.1|2127610_2128036_+|arsenate-reductase-(glutaredoxin)
MDVVIYHNPDCGTSRNTLAMIRNAGIEPHVVEYLKTPPARPLLVRMLARAGLSVRDVLREKGTPFAELNLGDPALTDAQLLDAIEAHPILINRPLVVSPKGVRLCRPSEAVLDLLPGQQGEFVKEDGERVVDEHGRRVATA
>NC_010172.1|WP_012253455.1|2127083_2127611_+|arsenate-reductase-ArsC
MPEPVYNVLFLCTGNSARSILAEAMLNKEGAGRFRAFSAGSQPKGEPHPLALQVLRESDHPTEGLRSKSWGEFAGPDAPVMDFVFTVCDNAAGEACPFWPGQPVTAHWGIEDPAAAEGSEMERKRAFVTAQRYLKNRISALVALPLGSLDQVALSSKVREIGQLAGATSARPEVA
>NC_010172.1|WP_042509172.1|2126731_2127091_+|helix-turn-helix-transcriptional-regulator
MDETQALAAFLALGQEHRLRLVRALVTAGPGGLASGVLADAVGVSAATVSHHLKELSHAGLVTSRREGRSIIYSAAYPALSDLVQFLMRDCCQGHPEVCAPALAALTACDCTTGDTLHA
>NC_010172.1|WP_012253464.1|2136737_2137847_+|DNA-binding-protein
MVAVNPVWQAADRIRARPAGQDGRKERVSVRSVRKELGKGSFSDIARELKVWREREDYHPVIEQADLPEAFERRLTVLGRELLEMARVEVARATLADFAGSERRRSAERDLLDEAFDRVDFLEERVAALQAELDRRPAGTGRGQAAPAQGAPAAEPEREWKGSPFADMAQGISLAKRADVFWEQVRIAVEDVFRRRGPLAVHPLFKSLPKQLKQDGEQVGFPLTEGWLRYHLLHLVQGGGLALDGYRFGLAEPAPGPDDGPAVDASAADDAAAMGRRRFWRQYMLDVHDLLVKEGPMTVAGIIDGMGPGWVEASERYRKITVGRIRYKLRGRIVEGRPFVELADGRFAAVPVEGHWDGLSAARTAEGNG
>NC_010172.1|WP_012253465.1|2137891_2138248_+|hypothetical-protein
MTPQEINAKVRGAYVRALQSSTMYSMSGSRIDDLFRDVKLYGRDAGTDFAETNLGRIVEEAARMAGCKRPCLEIQVYGWGRAALDGLAQTLRDRTGLKVDVAGPTVRLDWSEDKAGPV
>NC_010172.1|WP_012253466.1|2138271_2139024_-|toll/interleukin-1-receptor-domain-containing-protein
MRARARTHTEKPGPTSKTAAKAKPGPRSPARPAVPRPKPEKPEVRIFVSYSHRDADARQRLETHLAPLKREGVTTWFDGDMNAGDKLDTGIARALRNAHVFVGLLSPDYLASNYCWKIEYKRAMGRRANGTMRVVAVVLRPCDWKSTTAAGFKLLPEDGKEVTRWRSADDAFLNVAQGIRRVVADVRKTMAAAPPKPAPRKPAASKAAAQPPTKPGPKPRQKPGPKPNPGRAGAGAVARKRKPPAKGRKP
>NC_010172.1|WP_012253467.1|2139228_2139525_+|hypothetical-protein
MRMETAREALAQGRCLEVRYDDYARVVEVHACGFTGQGHAVMRVWQVRGGSVSGERTGWKLLRLDEALALAVTGEASEAPRRGYRRGDAGMARIVAQV
>NC_010172.1|WP_012253468.1|2139527_2140079_-|metallophosphoesterase
MPNTFFTSDSHFGHASVLAPRMDRPRPFATIAEHDEALIAAWNAVVRPGDIVWHLGDFAYRCDLAYAASIKARLNGRIRLIRGNHEELGGQLDWDKPVVDVQRVFVQDPGMPKPQAIWCSHYAHRTWPHERHGDLHLYGHSHGSLPGTRTSLDVGVDCWDWAPVRLDEILKRMAATPEPVPAA
>NC_010172.1|WP_012253470.1|2141511_2142120_+|glutathione-S-transferase-family-protein
MITVFHAPRTRSLRVLWMLEEMGLPYEVRLVDFPRHRSDAEFMRLNPAGSIPVLRDGDVVISESTAIIEYLIARYGPTPLAPQPSEAAYPSYLQYFHFGEASLAGPMTVVAHCWFMGPEDQRENAGVEAARTIFRARLPAIQSRLEEHDYLAGSAFTAADVSVVYALGLGQALRAVETYAPVIQEYLARCRARPGFQRASMK
>NC_010172.1|WP_012253471.1|2142296_2143493_+|FIST-C-terminal-domain-containing-protein
MAHGTQMRGHLCGIQTAWTDAVDALGAVAAVAGAIDRSVVGHLIVFFSPAYDAEILTAALAAQFPGVGLTGCSTSGGISPAGSIDRGLVAIAFPRQGFRIVSGLLTDIARLDVEGAASTVRGLRRTLDGGRPDGGSGHRFALSLIDSLANAEETVVSAVAWALDGIPLIGGSAGDDLAFRSTVLIHEGQVCREAAVILLVETDFPIRIFKSDNFEPTNRKFVVTAAREDERRVTELNAEPAAREYAMAVGLDPENLSTMSFAAYPLAVKIGGEYYCRSISRVEPDGSMTFFCAIGEGVVLTLAQPRDIVEATRAELESLDASLGGLDLVIGFDCVFRRLDAESRQVRHRIADLYRRYGVVGFETYGEQYRSMHLNQTFTGIAIGRAEAPERRDAKIAT
>NC_010172.1|WP_190274614.1|2143524_2144868_+|hybrid-sensor-histidine-kinase/response-regulator
MEPPAEPQTIEALTHRVAKLERINAALMAHVERTMDQQGGAYSLFQTAIMLEGRVRARTEELTALMNSLERSNAAMQAAKEEAETANRSKTRFLAAASHDLLQPLNAARLSLSALTDLAPGPEAQGIARQVERGLETIEDLIKALIDISKLDAGIVRPVIKPVRLADLVAGIEASFRPFAERKELRLVTRCADLVVETDGILLQRILQNLVSNAIRYTESGGVLIAARRRDKLCRIDVVDTGCGIPEAERALVFEEFFRGARECDDGGIGLGLGLSIVQRMASTLGHAIELHSRSGHGTRLSLTLPLATQRPDPRVTLAPLATALTGARVLAIENDASTAEALQRLLRNWDAEVQVFRDLAGVVAALGAGLARPDVMVIDYHLDNGACGLDVVDYLRRNRGWVTPVILTTADHGADIAARAQATGAELVHKPIKPAQLRSLLAYMLA
>NC_010172.1|WP_012253473.1|2144890_2145214_-|hypothetical-protein
MRRVLLASLTTLAVLAALPARAESPEGARHAAWQVCLDEAFAEQILTTSRSFAATKAVSTCRDREEAYLGVLAGSPLLDGEDVTRIRPALVARARDRLMGERRFSAL
>NC_010172.1|WP_012253474.1|2145526_2146195_-|transglutaminase-like-cysteine-peptidase
MRIAFSKLCVVRPALTTRLLAGLALAGALLLAAAPRAEAQPTAALPAMSLSAVTTGPARPIAAWAEFCERYPGECAVDRTEPASITLTPALWQTINAVNRRINRSVEAVTDGDHWQAADRWDLAEDGRGDCEDFQLLKRRLLVEAGLPRRAMRMTVVIDEKGEGHAVLTLITNRGDLVLDNKTNAVLPWRQTGYTFVKRESQEAVGWMALGRDASPLTAANR

Crispr_ID: NC_010172_3

• CRISPR_ID: NC_010172_3
• CRISPR_location: 2700341-2700612
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 4 spacers

Consensus repeat of NC_010172_3

Consensus_repeat	Method
GGCAACAACGTCGTGCTCGGCGG	CRISPRCasFinder

spacers of NC_010172_3

>3.1|2700364|31|NC_010172|CRISPRCasFinder
CACGGGCCGCGACACGATCACCATCGGCGAC
>3.2|2700418|25|NC_010172|CRISPRCasFinder
TAGCGGCCAAGCCGATTTCCAGGCC
>3.3|2700466|46|NC_010172|CRISPRCasFinder
CATCAGCCCGGAGGTCGGCGACAAGGACACGATCGCGCTCGGCAGC
>3.4|2700535|55|NC_010172|CRISPRCasFinder
TGCCGGCGGCGACAGCATCACCGCGGGCCTCCGCAACGGCGTGAAGCTCGGCACC

CRISPR arrays and Neighbor proteins around NC_010172_3

CRISPR arrays

The CRISPR arrays of NC_010172_3

>merge|NC_010172|3|2700341-2700612|CRISPRCasFinder
GGCAACAACGTCGTGCTCGGCGGCACGGGCCGCGACACGATCACCATCGGCGACGGCAACAACGTCGTGCTCGGCGATAGCGGCCAAGCCGATTTCCAGGCCGGCATCATCGTCGAGATCGCCAGCATCAGCCCGGAGGTCGGCGACAAGGACACGATCGCGCTCGGCAGCGGCCGCAACACCGTCATCGGCGGTGCCGGCGGCGACAGCATCACCGCGGGCCTCCGCAACGGCGTGAAGCTCGGCACCGGCACCAACGTCATCCTCGGCGA

>NC_010172|3|3|2700341-2700612|CRISPRCasFinder
GGCAACAACGTCGTGCTCGGCGG	CACGGGCCGCGACACGATCACCATCGGCGAC
GGCAACAACGTCGTGCTCGGCGA	TAGCGGCCAAGCCGATTTCCAGGCC
GGCATCATCGTCGAGATCGCCAG	CATCAGCCCGGAGGTCGGCGACAAGGACACGATCGCGCTCGGCAGC
GGCCGCAACACCGTCATCGGCGG	TGCCGGCGGCGACAGCATCACCGCGGGCCTCCGCAACGGCGTGAAGCTCGGCACC
GGCACCAACGTCATCCTCGGCGA

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
NC_010172.1|WP_042508838.1|2658522_2660910_+|tryptophan-7-halogenase	unknown	unknown	gnl|CDD|368139
NC_010172.1|WP_012253831.1|2657833_2658493_+|uridine-kinase	unknown	unknown	gnl|CDD|181310
NC_010172.1|WP_012253839.1|2715124_2716504_+|B12-binding-domain-containing-radical-SAM-protein	unknown	unknown	gnl|CDD|223963
NC_010172.1|WP_012253835.1|2662863_2663793_-|helix-turn-helix-domain-containing-protein	unknown	unknown	gnl|CDD|236612
NC_010172.1|WP_003600394.1|2722188_2723460_+|ATP-dependent-Clp-protease-ATP-binding-subunit-ClpX	unknown	unknown	gnl|CDD|235422
NC_010172.1|WP_012253838.1|2714194_2714986_+|methyltransferase-domain-containing-protein	unknown	unknown	gnl|CDD|225136
NC_010172.1|WP_012253840.1|2716500_2717814_+|B12-binding-domain-containing-radical-SAM-protein	unknown	unknown	gnl|CDD|223963
NC_010172.1|WP_012253843.1|2726449_2726716_+|hypothetical-protein	unknown	unknown	unknown
NC_010172.1|WP_012253842.1|2719742_2721179_+|trigger-factor	unknown	unknown	gnl|CDD|234956
NC_010172.1|WP_012253833.1|2660906_2661782_+|hypothetical-protein	unknown	unknown	unknown
NC_010172.1|WP_012253828.1|2654131_2656018_-|efflux-RND-transporter-periplasmic-adaptor-subunit	unknown	unknown	gnl|CDD|273776
NC_010172.1|WP_012253834.1|2661799_2662732_+|radical-SAM-protein	unknown	unknown	gnl|CDD|275338
NC_010172.1|WP_012253837.1|2713374_2714190_+|2OG-Fe(II)-oxygenase	unknown	unknown	gnl|CDD|379309
NC_010172.1|WP_003600393.1|2723727_2726148_+|endopeptidase-La	unknown	unknown	gnl|CDD|223542
NC_010172.1|WP_003601237.1|2664727_2665528_+|SapC-family-protein	unknown	unknown	gnl|CDD|377801
NC_010172.1|WP_012253841.1|2717921_2719361_-|multicopper-oxidase-family-protein	unknown	unknown	gnl|CDD|225043
NC_010172.1|WP_012253830.1|2656784_2657837_+|methyltransferase-domain-containing-protein	unknown	unknown	gnl|CDD|366359
NC_010172.1|WP_012253827.1|2651972_2654120_-|PqqD-family-peptide-modification-chaperone	unknown	unknown	gnl|CDD|377508
NC_010172.1|WP_003600395.1|2721310_2721937_+|ATP-dependent-Clp-protease-proteolytic-subunit	unknown	unknown	gnl|CDD|178955
NC_010172.1|WP_003606160.1|2656101_2656398_-|DUF3467-domain-containing-protein	unknown	unknown	gnl|CDD|371814

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
NC_010172.1|WP_042508838.1|2658522_2660910_+|tryptophan-7-halogenase	gnl|CDD|368139	pfam04820, Trp_halogenase, Tryptophan halogenase. Tryptophan halogenase catalyzes the chlorination of tryptophan to form 7-chlorotryptophan. This is the first step in the biosynthesis of pyrrolnitrin, an antibiotic with broad-spectrum anti-fungal activity. Tryptophan halogenase is NADH-dependent.	1.02701e-80
NC_010172.1|WP_012253831.1|2657833_2658493_+|uridine-kinase	gnl|CDD|181310	PRK08233, PRK08233, hypothetical protein; Provisional.	2.09672e-17
NC_010172.1|WP_012253839.1|2715124_2716504_+|B12-binding-domain-containing-radical-SAM-protein	gnl|CDD|223963	COG1032, COG1032, Fe-S oxidoreductase [Energy production and conversion].	1.57576e-20
NC_010172.1|WP_012253835.1|2662863_2663793_-|helix-turn-helix-domain-containing-protein	gnl|CDD|236612	PRK09685, PRK09685, DNA-binding transcriptional activator FeaR; Provisional.	2.8667e-20
NC_010172.1|WP_003600394.1|2722188_2723460_+|ATP-dependent-Clp-protease-ATP-binding-subunit-ClpX	gnl|CDD|235422	PRK05342, clpX, ATP-dependent Clp protease ATP-binding subunit ClpX.	0
NC_010172.1|WP_012253838.1|2714194_2714986_+|methyltransferase-domain-containing-protein	gnl|CDD|225136	COG2226, UbiE, Methylase involved in ubiquinone/menaquinone biosynthesis [Coenzyme metabolism].	1.15737e-17
NC_010172.1|WP_012253840.1|2716500_2717814_+|B12-binding-domain-containing-radical-SAM-protein	gnl|CDD|223963	COG1032, COG1032, Fe-S oxidoreductase [Energy production and conversion].	8.33949e-25
NC_010172.1|WP_012253842.1|2719742_2721179_+|trigger-factor	gnl|CDD|234956	PRK01490, tig, trigger factor; Provisional.	4.59294e-156
NC_010172.1|WP_012253828.1|2654131_2656018_-|efflux-RND-transporter-periplasmic-adaptor-subunit	gnl|CDD|273776	TIGR01730, COG0845:_Membrane-fusion_protein, RND family efflux transporter, MFP subunit. This model represents the MFP (membrane fusion protein) component of the RND family of transporters. RND refers to Resistance, Nodulation, and cell Division. It is, in part, a subfamily of pfam00529 (Pfam release 7.5) but hits substantial numbers of proteins missed by that model. The related HlyD secretion protein, for which pfam00529 is named, is outside the scope of this model. Attributed functions imply outward transport. These functions include nodulation, acriflavin resistance, heavy metal efflux, and multidrug resistance proteins. Most members of this family are found in Gram-negative bacteria. The proposed function of MFP proteins is to bring the inner and outer membranes together and enable transport to the outside of the outer membrane. Note, however, that a few members of this family are found in Gram-positive bacteria, where there is no outer membrane. [Transport and binding proteins, Unknown substrate].	8.58562e-25
NC_010172.1|WP_012253834.1|2661799_2662732_+|radical-SAM-protein	gnl|CDD|275338	TIGR04545, rSAM_ahbD_hemeb, heme b synthase. Members of this family are AhbD (alternative heme biosynthetic protein D), a radical SAM enzyme in sulfate-reducing bacteria and methanogens that performs the last decarboxylations to synthesize heme b from Fe-coproporphyrin III. Members include DVU_0855, previously included in error in TIGR04055, the NirJ2 family thought to be involved in heme d1 biosynthesis. [Biosynthesis of cofactors, prosthetic groups, and carriers, Heme, porphyrin, and cobalamin].	2.01619e-22
NC_010172.1|WP_012253837.1|2713374_2714190_+|2OG-Fe(II)-oxygenase	gnl|CDD|379309	pfam13640, 2OG-FeII_Oxy_3, 2OG-Fe(II) oxygenase superfamily. This family contains members of the 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily.	6.90347e-05
NC_010172.1|WP_003600393.1|2723727_2726148_+|endopeptidase-La	gnl|CDD|223542	COG0466, Lon, ATP-dependent Lon protease, bacterial type [Posttranslational modification, protein turnover, chaperones].	0
NC_010172.1|WP_003601237.1|2664727_2665528_+|SapC-family-protein	gnl|CDD|377801	pfam07277, SapC, SapC. This family contains a number of bacterial SapC proteins approximately 250 residues long. In Campylobacter fetus, SapC forms part of a paracrystalline surface layer (S-layer) that confers serum resistance.	2.50941e-91
NC_010172.1|WP_012253841.1|2717921_2719361_-|multicopper-oxidase-family-protein	gnl|CDD|225043	COG2132, SufI, Putative multicopper oxidases [Secondary metabolites biosynthesis, transport, and catabolism].	1.00369e-50
NC_010172.1|WP_012253830.1|2656784_2657837_+|methyltransferase-domain-containing-protein	gnl|CDD|366359	pfam00891, Methyltransf_2, O-methyltransferase. This family includes a range of O-methyltransferases. These enzymes utilize S-adenosyl methionine.	1.27757e-23
NC_010172.1|WP_012253827.1|2651972_2654120_-|PqqD-family-peptide-modification-chaperone	gnl|CDD|377508	pfam05402, PqqD, Coenzyme PQQ synthesis protein D (PqqD). This family contains several bacterial coenzyme PQQ synthesis protein D (PqqD) sequences. This protein is required for coenzyme pyrrolo-quinoline-quinone (PQQ) biosynthesis.	4.47038e-07
NC_010172.1|WP_003600395.1|2721310_2721937_+|ATP-dependent-Clp-protease-proteolytic-subunit	gnl|CDD|178955	PRK00277, clpP, ATP-dependent Clp protease proteolytic subunit; Reviewed.	2.92817e-146
NC_010172.1|WP_003606160.1|2656101_2656398_-|DUF3467-domain-containing-protein	gnl|CDD|371814	pfam11950, DUF3467, Protein of unknown function (DUF3467). This family of proteins are functionally uncharacterized. This protein is found in bacteria, archaea and viruses. Proteins in this family are typically between 101 to 118 amino acids in length.	2.09627e-15

Protein sequences

>NC_010172.1|WP_003601237.1|2664727_2665528_+|SapC-family-protein
MSKQLLIYERAVPVTRQRHGSWSVKAGASFDFARGVNSVPLMVAEFPNSAAEYAIVFGGAADEIIPVALLGIRDNENLYVSESGSWSGSYVPAFLRRYPFVFSSDNANDADATFTLCVDEDFSGCNDEGRGERLFDADGERTQYLQNVLGFLQAYQVQFQRTKLFVKRLQEFGLLEPMQAQFTLRSGQRSTLSGFSVVSRDRLKALSAEQLAELMQADEMELIYLHLASLRNLAPIAERIGAPAESAQADETAPASPADFETSGNA
>NC_010172.1|WP_012253835.1|2662863_2663793_-|helix-turn-helix-domain-containing-protein
MVGPLFEPRAFHPGQQSPTGSASGAIIGDIMVARVVFGAQSYRRDRDLIARTPDHILFHLYNVGGFSGLISGQQTTIWSSQVAIIDLAQEIDTLAASSDTVALIVPRALLPGLADNPLPPRLDAGRNRLLAAHLIALRERSTLIEEPDVEEVVTQTLDFLRALLDPSRAEETAAAPTLATCHLTLAEQTIRTHLASPDLSPEMIANEIGVSRATLYRMFAPYGGIMRSVQERRLLAVRAALSDPLETRRLSRLATDFGFRGKVHFSRSFRAQFGITASEFRAEQVALAQRDARSDLGVLHDWWPRLGNR
>NC_010172.1|WP_012253834.1|2661799_2662732_+|radical-SAM-protein
MGRYDHAAYVSLTMEFRCNLKCVHCMIEGTMDRLAPESDAHFEEILALNVRERRWTGLILTGSEITLRRDLPELARRARASGFAHVRIQTHGMHLGQPSYCRRLVEAGVDEFFVSIAGSDAASHDALTLVPGSFERALRGLETLDAMEGVETLTNTVVTERSYRLLPDLVTRLGHLQRLTQMEFWVYFPMSERDEKGLVARHADVLPYLREAVLRARALGRAVEVKNFPECLLGDLGNALVNGQPELHIDPDFWREFERNGFYQCAHRESCSSRECLGLNTAYVAKFGLEADLVHPLPAPRRAAWSRAPA
>NC_010172.1|WP_012253833.1|2660906_2661782_+|hypothetical-protein
MTGLDPARLELSAPGDRYADYCLWDYEPVGPTAGRLRQASLLWHSLACAGADPRLFAICDALRDGLGPGRSVWGAKLRDGVTTWEFYFYDYARLDRTVSIERVLAILAPFARCDLRYAGARPYFMFSLDLDDALARGERSLDRLNIYVGNPGSSVSSGLSYGLTADGLVFDNLYSFFDAARERNAIAAKAACSAHFDLPGLDLDAILWPELMRCGVVVVANKRLCDGVYFSRVGIDGLIAFLDRLAYPPAIRDFVRSERRRLSHLLFDVGIDYAMIDGRPTVTKSAYYGLL
>NC_010172.1|WP_042508838.1|2658522_2660910_+|tryptophan-7-halogenase
MFRSERSAPPRLVVAGGGPASWMAALYLKRVLRRAGWTVTLVGSAPAAGASAALATRPAFTRFLKGFGIDEAIFMRRCAATYRLASRYDDWFAEGQGHWHPFGACGPRIAGRDLFHYWMKLRGEGAEDEAGRYADYAPQALMAAEARGPRPLADRSSLIDSGDYGYHLDRKALVRFLRELALSEGVRGVTGRVREIERNLYGDVGALILDGGQTIEGDIFLDCTGAAAQLIGTALDEAWVAGSGPGDRVARLSLPRSPEVPPFTAYRGRAEGWTASLPLADRTEHLFVYDSRTTPPDAAAATLRRAFDGQADIVDAELRHGRRASPWQRNVVALGAAAGAVEPLLGFDLDLVLAGLETFLAYLPRQGEGGDVLRRAYAARLNRFHDDAAEAVAAHYVLGRRSEPFWAAARAVMIPDRLADRLDLYELAGHVALSEEAPFSEGDHYLLFSGADFLPRRPFAPVDVADGREIARLLASIRAQSAQIAGAMAPHVRLMDALHGQAPVAAPAVRIAAASAPAGPAALRRTPEGARLADLVAGLGQPFGYERSVKASPAGLQTDRFLMSLHRASLGLTPETTLDRLAAGLGLPERERAEAAGLIGGADILHLGYEDGPSGALYKLYVEWSSRTDAAWTGADEAGAEPMLVHRAYKWNPGGTHPPVVTLYHWPWVRGPEEIEARLTRMTGAWGPAGAPARDTAHAILLLAQERGRGAVHYLEAREEPGLRLSYDLNLYACGLTVADAEPLLGQAFVDLGVPRDAAAMVLRERLQETLGHVAGGVGRDGQPFVTVYSGVAGG
>NC_010172.1|WP_012253831.1|2657833_2658493_+|uridine-kinase
MSAPFVLAVSGPPGSGKTTLSHALSERFGGAPMLAYDAYEEITGWPPERVAAWLAGGAPLDAVPVPGLTEDLARLRRGEPVPDRERGGTLRLSRHAARPVIVLDTLLGRAHPGTGAQIDHLVWLDLPLDVALARKLRSFTGAAGRDPTAAPGLLEALDAYLGRYDALLHPTYALQRERVRPAADQILGAGTLAAHLDAIQSAIQSTLHATLTAVPTPTA
>NC_010172.1|WP_012253830.1|2656784_2657837_+|methyltransferase-domain-containing-protein
MSAPELPGPEFPRVDRFLESEAAAHALSFALTSGLIDRLARRADTAISGLADEFRLDRPQLACLTGLLVGAGIAAEPAPGRIGLSEAFRAVLPCRDLIEAKLAFAAAASEDLRRHFPAFVADLPAFMAASRTFELFRYDRCFEITPDNLDATRRWVAYTTCLTRYEAGPGLDRIEPGRHRRLLDLGGNSGEFAAQACRRAPDLTATVFDLPVVCALGRENLADRPEGARVAFQAGDMRRDPLPEGHDLVTFKSVLHDWPAGEARALLERAARALAPGGRLVIYERAPMALRERPLGYVQSADLVFQPFFRDADLYTETLAALGFVDCSVRHLAFETPFHLIEARKPGGAR
>NC_010172.1|WP_003606160.1|2656101_2656398_-|DUF3467-domain-containing-protein
MSESSKPGAADQVRAIEWREDSMSTQFANVVNVQGTREQVDLFFGTNRTWNLAETESVVVDLSNRVILTPLAAKRLHSVLGGVLTEYENRHGRLDLDA
>NC_010172.1|WP_012253828.1|2654131_2656018_-|efflux-RND-transporter-periplasmic-adaptor-subunit
MSERLAVEGASAASPAARLSLLEPALWQVLRTSQTLQAAAEAWLTLQAPMLGAPRACVLLPGTSVGLAPLAVFPAQSECEAGLRETAEAAFRENRPVVQAMAEAGPHPGAAPQGSGAWIALPLVLDEACFAVTAFALDSLDKDELRAAIRQVQWGSAWLVAKQAELIGRGERRTLARSRSVLDLLAGVLEQSRFSAACTAAVTDLAIAFSCQRAAIGFVRRGSARIAAISHSAQFGREMNLVRRLGACMNEAIDQRSLILFPPPENEATVTTAHADLARLQYGGRVLTVPMLVDDRFVGAITLERPADQPFSLDIVEQVSACAAAIGPVLEDKRQNDRWLVLKATDAVMNGARLVFGPNHTGLKLSLAAVLAVVLALSVIKIDYRVTADARVEGLVRRSVVASYDGYLKTAGHRAGDTVRKGDLLAALEDRDLALERLRWVTERQQRTYEYDKALATRQPATINVVKSQIEQADAQIRLLDEQIARSKFTAPFDGLIVSGDLSQSIGGAISRGQVLFEIAPLDSYRVVLSVDERLIADLREGQTGHVLATSLPDQPQVLTIQTITPVAEAKNGRNLFRVEGRIEDGATRLRPGMEGIAKVDVDRRLLAWVWARPIVDWVRLALWHWWP
>NC_010172.1|WP_012253827.1|2651972_2654120_-|PqqD-family-peptide-modification-chaperone
MSQSLFSQSWYRVAALRPRLRRHAEIHRQSFRGEVWYVLQDHQTGRFHRLSPAANLILCMMDGRRTLQEVWEAAARRNEDDPPTQDDTIRLISQLHGSDLLQGDLPPDLAELAERSESTARRTLIARVKNPLALRFPLFDPDPLLDRLAPYFRPLFSVWGLAVWLAVVISGLVVAGLHAGELTGDVAGQMLSAQNVALMMCLYPVIKALHEAGHACAAKAWGGEVHEVGLMLLVLFPAPYVDASTSAAFPSKWQRMMVAAAGIFVELFLAGLAAIVWATAEPGLVRAAAFNVMVIGSVSTLLFNGNPLLRFDGYYIFADLIEIPNLGSRANRYVFYLIERYALKIEGAKSPVTADGERPWLFVYAVAAFVYRTTVSIAIASFIATQYFVFGVLLAGLALFGTVVSPLVKGLKFILTDPKLNRRRSRAVTLTSAATGAVLALLFVVPLPYTTMAQGVVWAPDDTQIRTRTDGILTTLLVEPGRDAQAGEALATLEDPSLDSRVAVLEAQLAELRLRYDAARLGDRVQAQILQEQIASTQEILRVYRERQAGLIVRASQAGRLIVPGAVDLPGRYLRRGERIGYVLTADDPVVRVVVPQTDVDLIRSRTQGVAARLAEKPEEVRAGSILREVPGAQQEIPSLALATQGGGTIAVDASNPQKPVALQSIFVFDVQLAGGLPFNVLGERVYIRFDHGAEPIAWRALRSLRQVFLSQFRV
>NC_010172.1|WP_012253837.1|2713374_2714190_+|2OG-Fe(II)-oxygenase
MPFLQTISIDAAEASAHADALTRLREDALQAVVIRNVLTPTECASIVADLAANRQGFPTTSFPAPFRSHFYGMNLNLADPDLRAYFDMAPGFTRSLSELMAPWGGFETRILALLSRLDGGRPYGPPALHDGRTYMATTLRSHHEGGYIPPHFDNEQKNRPSYRHLESVVEGDIFSFVLTLSKAERGGMLEVFDAKAEAWSGRFQNRDRAREKPDLARFARHAFDVEAGTIVLLRSGRFLHQVTPVEGARLRWTACSFMARSRTGDAVHCWG
>NC_010172.1|WP_012253838.1|2714194_2714986_+|methyltransferase-domain-containing-protein
MARIPAYFDAFLAAVAEGAAIEHVHLGHWDEPGAVGPTRARAGFAQAQERLSAALVAWLDLAPGHRVLDVGCGFGGTLRAIRRSDPEVDVIGLNLDPRQLAVGLRQDNRPRNAAWVAGDACRLPFAEAAFDRLLCVEAAFHFASRRAFFGEAQRVLTREGVLVMSDIVLTRPPLPPERVAPFADGLEAAFGPWPDKWAGLDVIRRDAEAAGFTLRVTDATANTEPSYHTIASDRRGRPTGGDVLRELHRLGCLHYLYCRLDKR
>NC_010172.1|WP_012253839.1|2715124_2716504_+|B12-binding-domain-containing-radical-SAM-protein
MLEPSSPTHRRIALVCMTPRPDAQELGELLLPSYGIRRIHAALLGDPDLADASVRLFDRRTADIEAYVAEILDFEPDLVGFSIYVWSAPAMIAVARALRARRPSLAIVFGGPSARSAFFDLEPYRPAHAYLDAVVEGDGEEIICEIARLPQLDRAAWERVRGLTLPTATGWLRTERRPPITALDRIASPYAHGLMPPGGVAYLESFRGCPLSCRFCEWGTKENSKASFSADYIARELDAFRTLEAPAVFLLDAGLNLNIGAFRNLRLANQRSGFLKETLLWAEIYPSIVRDEHLAFIEEIGTAYLGVGMQSMDPAVLRLHDRPSDSPRFEAAVRALARVTNIELQIIAVLPGDTPEGFFRTLDYALSLPASVRVYHCLVLPDALLTRSRPEWNVRFDPHTLTMRSCEGWSEDAVARTRAELRRRALAAGGKAGEFWWAFPRQPERVTQARASWRQAAAR
>NC_010172.1|WP_012253840.1|2716500_2717814_+|B12-binding-domain-containing-radical-SAM-protein
MKAARSGLRIALVAQYPERDPAMPSFVPNLGLRMVEATLRAAALPGLVCRVWDLTGGDPERVAREITAFDPDIVGFSAYLWSLPFLCRVAALIKRDDPARLVVFGGPSARPVMFARPPFSKAADDIDLLVINEGEETFLEIVSLEQRTPAALGTLRGVAVRDGAAWRETPARPLANLDSLASPYALNLVQHGGLGVLQTYRGCPFTCSFCEWGTMESPKRVRAVDHLTQEFEAIAGHDVYAALLVDAGLNLNRNAFLNLRRAAEESGFFERHGLICEVYPAAVRQEHLDFLATVSNAYVGIGLQSFDNAVLAHVDRTYDERRFDETFAKLDAVASLAVEIILGLPGDNPETFRRNFERARRLPCALRVYHCVVLPSGLMVRSPPEHRLDYDPVSLKMLSCTGWSEAALAAECRFLTRQASLQGGRSGEFFWTFPPPR
>NC_010172.1|WP_012253841.1|2717921_2719361_-|multicopper-oxidase-family-protein
MRNPDPTAQPAPGSFSRRELFAGSAVLALMPRTAAAQAPAGKAPPSEPKSIKAAPAKLRLKPEPAPETAVWCLGEAEAPILRVKLGEAIRLRVENGTDKPLSLHWHGVRIVNAMDGVGGVTQEPIKPGASFTYDFTPPEAGSFLIRALVVGGSSEPSGRGIAGMLIVDEPSPPPVDADLALLVQDWRLDEAGALQPFGQVAFAAAAGRLGSVVTLNGRPIPLALDGRPGSRLRLRLANACNARSLRIRFDGLKAYVAAVDGQPTDTFEPLKATLPFAPGTRYDLLLDLPAQAGPAGTITALVGQGLPLATLTAVGEPVAQSGRAAIGSIPENKRLPAEIRLQSALRRELVLTGGVRPDKAKPGAETPYSGDPAKIWQINGASGTAGASPLFSVKRGGVVVLSIRNDTGFIQSLHLHGHVFRLLHQLDDGWEPYWLDTVQIPEGKSAQIAFVADNPGRWLLSATVLERFDTGLWTSFEVS
>NC_010172.1|WP_012253842.1|2719742_2721179_+|trigger-factor
MQVTETTSEGLKREFQVLLPANELEDRLNTELSNIKGKVQIKGFRPGKVPVAHLRKVYGKSVMADVLQNAVNEANQQIVTDKGLRLALEPQIEFPKDEEQTIIERALDAKGDLAFKVKLEVLPSFELADLSDVSIKKLVLKPSDEEVNETLERMAKDSRSFEPREEGAEAQSGDRVTIDFVGRIDGTEFEGGKGEDVDLELGSNTFIPGFEDQLVGAKVGDSRLVKVAFPADYQAEQLAGKDAEFDVTVKAVAAPGETKIDDELAKRFGMDDLEKLKEAVSKAVGSDYEAQSRRKLKKELLDALDGKYAFDLPPSLVHQEFAAVWAQVEQDLKTRGKTFEDEGTTEEASQAEYRKIAERRVRLGLVLAQVGETADIKVSDDEVNQALFARIRQFPGQEKQVYDFYRNNPQALAELRAPLFEEKVVDHVLGQVQVVEEPVSKEALFAEDDEADAVTGGAATDEKPSESNNEAAADKAAG
>NC_010172.1|WP_003600395.1|2721310_2721937_+|ATP-dependent-Clp-protease-proteolytic-subunit
MRDPVDYFHNSLVPMVVEQSSRGERAFDIYSRLLRERIIFLTGPVEDQGASLIVAQLLFLEAENPKKEISFYINSPGGVVTSGLSIYDTMQFIRCPVTTLCVGQAASMGSLLLAAGEAGHRFALPNARIMVHQPSGGFQGQATDILIHAREIEALKKRLNEIYVKHTGREYETIHQALERDNFMTADAAKEFGLIDDILHKRPEPAAA
>NC_010172.1|WP_003600394.1|2722188_2723460_+|ATP-dependent-Clp-protease-ATP-binding-subunit-ClpX
MSKTGGNDSKSTLYCSFCGKSQHEVRKLIAGPTVFICDECVELCMDIIREESKSSLVKSRDGVPTPKEIRRVLDDYVIGQDFAKKVLSVAVHNHYKRLAHATKHNDVELAKSNIMLIGPTGSGKTLLAQTLARILDVPFTMADATTLTEAGYVGEDVENIILKLLQASDYNVERAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKIMEGTVASVPPQGGRKHPQQEFLQVDTTNILFICGGAFAGLERIISQRGKGTSIGFGASVQAPDDRRTGEVFRSVEPEDLLKFGLIPEFVGRLPVLATLEDLDEEALKKILQEPKNALVKQYQRLFEMENVELTFQDEALSLVARKAIERKTGARGLRSILETILLDTMYDLPGLESVEQVVIGPEVVEGKSRPLFIHGDRNKEAPASVSA
>NC_010172.1|WP_003600393.1|2723727_2726148_+|endopeptidase-La
MTQSKSRQPVVPGSTGSYAVLPLRDIVVFPHMIVPLFVGREKSIRALEEAVRSDRHILLATQINASDDDPATDAIYTIGTLASVLQLLKLPDGTVKVLVEGAGRAQIESFVRSDEYYEAQALTLTDDLGDRVEAEALARSVISEFENYVKLNKKISPEVVSAVTQIDEPSKLADTVGSHLAVKIADKQAILEIPTVAQRLERVLSLMESEISVLQVEKRIRTRVKRQMEKTQREYYLNEQMKAIQKELGDEDGRDELAELEDKIEKTKLTKEAREKATAELKKLRQMSPMSAEATVVRNYLDWMLGIPWGKRSKIKKDLLGAQQVLDSDHFGLDKVKDRIVEYLAVQQRANKLTGPILCLVGPPGVGKTSLGKSIAKATGREFVRMSLGGVRDEAEIRGHRRTYIGSMPGKIVQSMRKAKTSNPLILLDEIDKMGMDFRGDPSAALLEVLDPEQNATFNDHYLEVDYDLSNVMFVTTANTLNIPGPLMDRMEVIRIAGYTEEEKLQIARRHLIPEAVKKHGLTPDEWSITDDGLMMLIRRYTREAGVRNLEREISNLIRKAVKEILITKVKQVEANPETLPEFLGPPKFRYGEIDADDQVGVVTGLAWTEVGGELLTIEGVMMPGKGKMTVTGNLRDVMKESISAAASYVRSRAIDFGIEPPLFERRDIHVHVPEGATPKDGPSAGIAMATAIISTLTGIPVRRDVAMTGEVTLRGRVLPIGGLKEKLLAALRGGIKTVMIPEENAKDIAEVPDSVKNGLEIIPVSRMDQVLQHALVRQPEPIEWDEPLPAKTPKRDEDGAAVIAH
>NC_010172.1|WP_012253843.1|2726449_2726716_+|hypothetical-protein
MPDPNPTVGELFKANVITHDDLNSLIDAVLAGRMNVREDLAEGYTLDVAAAVKANAFATAVLRDKTSTTGARRTATRTAILLARAQKA

Crispr_ID: NC_010172_4

• CRISPR_ID: NC_010172_4
• CRISPR_location: 4012808-4012892
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 1 spacers

Consensus repeat of NC_010172_4

Consensus_repeat	Method
AAAACAAAAGGCGAGAGCATCGT	CRISPRCasFinder

spacers of NC_010172_4

>4.1|4012831|39|NC_010172|CRISPRCasFinder
CCCGAAAGGTGGGCACCGGCTTTCGGAAAAAGATGATGC

CRISPR arrays and Neighbor proteins around NC_010172_4

CRISPR arrays

The CRISPR arrays of NC_010172_4

>merge|NC_010172|4|4012808-4012892|CRISPRCasFinder
AAAACAAAAGGCGAGAGCATCGTCCCGAAAGGTGGGCACCGGCTTTCGGAAAAAGATGATGCAAAACAAAAGGCGAGAGCATCGT

>NC_010172|4|4|4012808-4012892|CRISPRCasFinder
AAAACAAAAGGCGAGAGCATCGT	CCCGAAAGGTGGGCACCGGCTTTCGGAAAAAGATGATGC
AAAACAAAAGGCGAGAGCATCGT

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
NC_010172.1|WP_012254846.1|4006839_4007412_+|fasciclin-domain-containing-protein	unknown	unknown	gnl|CDD|225214
NC_010172.1|WP_012254861.1|4022089_4023595_-|SulP-family-inorganic-anion-transporter	unknown	unknown	gnl|CDD|223732
NC_010172.1|WP_012254855.1|4014594_4015299_+|amino-acid-transporter	unknown	unknown	unknown
NC_010172.1|WP_080518444.1|4011483_4011843_+|energy-transducer-TonB	unknown	unknown	gnl|CDD|372476
NC_010172.1|WP_012254857.1|4016381_4017272_+|16S-rRNA-(adenine(1518)-N(6)/adenine(1519)-N(6))--dimethyltransferase-RsmA	unknown	unknown	gnl|CDD|234708
NC_010172.1|WP_012254859.1|4018962_4020234_-|glycosyltransferase-family-2-protein	unknown	unknown	gnl|CDD|133045
NC_010172.1|WP_012254851.1|4010510_4011245_+|molybdate-ABC-transporter-permease-subunit	unknown	unknown	gnl|CDD|181841
NC_010172.1|WP_012254860.1|4020750_4021890_-|alpha/beta-hydrolase	unknown	unknown	gnl|CDD|223669
NC_010172.1|WP_012254845.1|4005795_4006716_-|SDR-family-oxidoreductase	unknown	unknown	gnl|CDD|235737
NC_010172.1|WP_003605770.1|4009946_4010180_+|hypothetical-protein	unknown	unknown	unknown
NC_010172.1|WP_012254847.1|4007658_4008495_+|alpha/beta-hydrolase	unknown	unknown	gnl|CDD|223669
NC_010172.1|WP_012254853.1|4012935_4014030_+|molybdenum-ABC-transporter-ATP-binding-protein	unknown	unknown	gnl|CDD|226628
NC_010172.1|WP_012254849.1|4009486_4009705_+|hypothetical-protein	unknown	unknown	unknown
NC_010172.1|WP_012254850.1|4010185_4010434_+|BrnA-antitoxin-family-protein	unknown	unknown	gnl|CDD|226045
NC_010172.1|WP_150105724.1|4014050_4014428_-|hypothetical-protein	unknown	unknown	unknown
NC_010172.1|WP_012254858.1|4017919_4018966_-|HlyD-family-efflux-transporter-periplasmic-adaptor-subunit	unknown	unknown	gnl|CDD|273776
NC_010172.1|WP_012254856.1|4015323_4016373_+|4-hydroxythreonine-4-phosphate-dehydrogenase-PdxA	unknown	unknown	gnl|CDD|235402
NC_010172.1|WP_042508953.1|4008907_4009426_+|GAF-domain-containing-protein	unknown	unknown	gnl|CDD|379056
NC_010172.1|WP_012254852.1|4012015_4012672_-|glutathione-S-transferase	unknown	unknown	gnl|CDD|198298
NC_010172.1|WP_190274556.1|4017638_4017809_-|hypothetical-protein	unknown	unknown	unknown

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
NC_010172.1|WP_012254846.1|4006839_4007412_+|fasciclin-domain-containing-protein	gnl|CDD|225214	COG2335, COG2335, Secreted and surface protein containing fasciclin-like repeats [Cell envelope biogenesis, outer membrane].	1.25977e-54
NC_010172.1|WP_080518444.1|4011483_4011843_+|energy-transducer-TonB	gnl|CDD|372476	pfam13103, TonB_2, TonB C terminal. This family contains TonB members that are not captured by pfam03544.	6.4057e-05
NC_010172.1|WP_012254857.1|4016381_4017272_+|16S-rRNA-(adenine(1518)-N(6)/adenine(1519)-N(6))--dimethyltransferase-RsmA	gnl|CDD|234708	PRK00274, ksgA, 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA.	3.21108e-116
NC_010172.1|WP_012254851.1|4010510_4011245_+|molybdate-ABC-transporter-permease-subunit	gnl|CDD|181841	PRK09421, modB, molybdate ABC transporter permease subunit.	8.00099e-126
NC_010172.1|WP_012254860.1|4020750_4021890_-|alpha/beta-hydrolase	gnl|CDD|223669	COG0596, MhpC, Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily) [General function prediction only].	1.48718e-26
NC_010172.1|WP_012254845.1|4005795_4006716_-|SDR-family-oxidoreductase	gnl|CDD|235737	PRK06197, PRK06197, short chain dehydrogenase; Provisional.	1.96726e-110
NC_010172.1|WP_012254847.1|4007658_4008495_+|alpha/beta-hydrolase	gnl|CDD|223669	COG0596, MhpC, Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily) [General function prediction only].	2.54391e-38
NC_010172.1|WP_012254853.1|4012935_4014030_+|molybdenum-ABC-transporter-ATP-binding-protein	gnl|CDD|226628	COG4148, ModC, ABC-type molybdate transport system, ATPase component [Inorganic ion transport and metabolism].	2.27639e-145
NC_010172.1|WP_012254859.1|4018962_4020234_-|glycosyltransferase-family-2-protein	gnl|CDD|133045	cd06423, CESA_like, CESA_like is the cellulose synthase superfamily. The cellulose synthase (CESA) superfamily includes a wide variety of glycosyltransferase family 2 enzymes that share the common characteristic of catalyzing the elongation of polysaccharide chains. The members include cellulose synthase catalytic subunit, chitin synthase, glucan biosynthesis protein and other families of CESA-like proteins. Cellulose synthase catalyzes the polymerization reaction of cellulose, an aggregate of unbranched polymers of beta-1,4-linked glucose residues in plants, most algae, some bacteria and fungi, and even some animals. In bacteria, algae and lower eukaryotes, there is a second unrelated type of cellulose synthase (Type II), which produces acylated cellulose, a derivative of cellulose. Chitin synthase catalyzes the incorporation of GlcNAc from substrate UDP-GlcNAc into chitin, which is a linear homopolymer of beta-(1,4)-linked GlcNAc residues and Glucan Biosynthesis protein catalyzes the elongation of beta-1,2 polyglucose chains of Glucan.	2.29298e-53
NC_010172.1|WP_012254850.1|4010185_4010434_+|BrnA-antitoxin-family-protein	gnl|CDD|226045	COG3514, COG3514, Uncharacterized protein conserved in bacteria [Function unknown].	1.86255e-11
NC_010172.1|WP_012254861.1|4022089_4023595_-|SulP-family-inorganic-anion-transporter	gnl|CDD|223732	COG0659, SUL1, Sulfate permease and related transporters (MFS superfamily) [Inorganic ion transport and metabolism].	9.61059e-141
NC_010172.1|WP_012254858.1|4017919_4018966_-|HlyD-family-efflux-transporter-periplasmic-adaptor-subunit	gnl|CDD|273776	TIGR01730, COG0845:_Membrane-fusion_protein, RND family efflux transporter, MFP subunit. This model represents the MFP (membrane fusion protein) component of the RND family of transporters. RND refers to Resistance, Nodulation, and cell Division. It is, in part, a subfamily of pfam00529 (Pfam release 7.5) but hits substantial numbers of proteins missed by that model. The related HlyD secretion protein, for which pfam00529 is named, is outside the scope of this model. Attributed functions imply outward transport. These functions include nodulation, acriflavin resistance, heavy metal efflux, and multidrug resistance proteins. Most members of this family are found in Gram-negative bacteria. The proposed function of MFP proteins is to bring the inner and outer membranes together and enable transport to the outside of the outer membrane. Note, however, that a few members of this family are found in Gram-positive bacteria, where there is no outer membrane. [Transport and binding proteins, Unknown substrate].	1.58143e-09
NC_010172.1|WP_012254856.1|4015323_4016373_+|4-hydroxythreonine-4-phosphate-dehydrogenase-PdxA	gnl|CDD|235402	PRK05312, pdxA, 4-hydroxythreonine-4-phosphate dehydrogenase PdxA.	0
NC_010172.1|WP_042508953.1|4008907_4009426_+|GAF-domain-containing-protein	gnl|CDD|379056	pfam13185, GAF_2, GAF domain.	7.89387e-08
NC_010172.1|WP_012254852.1|4012015_4012672_-|glutathione-S-transferase	gnl|CDD|198298	cd03189, GST_C_GTT1_like, C-terminal, alpha helical domain of GTT1-like Glutathione S-transferases. Glutathione S-transferase (GST) C-terminal domain family, Saccharomyces cerevisiae GTT1-like subfamily; composed of predominantly uncharacterized proteins with similarity to the S. cerevisiae GST protein, GTT1, and the Schizosaccharomyces pombe GST-III. GSTs are cytosolic dimeric proteins involved in cellular detoxification by catalyzing the conjugation of glutathione (GSH) with a wide range of endogenous and xenobiotic alkylating agents, including carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress. GSTs also show GSH peroxidase activity and are involved in the synthesis of prostaglandins and leukotrienes. The GST fold contains an N-terminal thioredoxin-fold domain and a C-terminal alpha helical domain, with an active site located in a cleft between the two domains. GSH binds to the N-terminal domain while the hydrophobic substrate occupies a pocket in the C-terminal domain. GTT1, a homodimer, exhibits GST activity with standard substrates and associates with the endoplasmic reticulum. Its expression is induced after diauxic shift and remains high throughout the stationary phase. S. pombe GST-III is implicated in the detoxification of various metals.	4.65157e-49

Protein sequences

>NC_010172.1|WP_012254852.1|4012015_4012672_-|glutathione-S-transferase
MITVHHLENSRSQRVLWLLEELGLDYTVKRYERDPATMQAPRELRAVHPLGKSPVIAADGQVVAETGAIVEFLTARADGALVPPAGTLERARYTYFLHYAEGSAMPPLLLKLIFSRLAPGSPALVRPLVRAIAGKVTGGFVDPELRRHAAYWEAELAERAYFCGADFTAADIMMSFPLEAFAARGTGAGPRLTDWLARIHARPAYKRALERGGPYAYA
>NC_010172.1|WP_080518444.1|4011483_4011843_+|energy-transducer-TonB
MAVTIMLLAWMMTSPDASAKSICRTWDGPEISEYKEKLYPIAVRLARSRIYAPHIDLVTVIAITIDKNGRLIASETARRSRSPWFDQEVLLAFPVGLRLPPLPACGPESIKAFIPIRVR
>NC_010172.1|WP_012254851.1|4010510_4011245_+|molybdate-ABC-transporter-permease-subunit
MGAVSGLADWPSAWLGLSPDETTALLLSLRVALVATLTSLPLGLLLAWLLARRRFPGHALLDGLVHLPLILPPVVTGYLLLLAFGRRGVFGAWLAEIGVVFSFRWTGAALACAVMGLPLMVRAMRLSIEAVDRKLEQAAATLGAAPPLVFLSVTLPLSLPGILAGAVLAFAKAMGEFGATITFVSNIPGETQTLPSAIYTLTQVPGGEAGALRLTLISVAVSMLALVASEGLARRMSRRIGAGR
>NC_010172.1|WP_012254850.1|4010185_4010434_+|BrnA-antitoxin-family-protein
MAIDRTRRPSDPRQAAEAAFRAVTTRKAPEETAPAPPRAPLIPGTRETVSLRIDRAVLEHYQKDGPGWQDRINAALKAAAGL
>NC_010172.1|WP_003605770.1|4009946_4010180_+|hypothetical-protein
MSDDIDAKTRAAERAERAERMAREGQQAMAEHLAAVKAVDERTVRLRALRLAKEAEEAAERAANPPAPKRGRKKATA
>NC_010172.1|WP_012254849.1|4009486_4009705_+|hypothetical-protein
MPVFVLRGACRAPDGQIGPPLFEETITAASPSEAVRLANAHDLSTKDERANALWLVDAQGVLHWSLRRADRD
>NC_010172.1|WP_042508953.1|4008907_4009426_+|GAF-domain-containing-protein
MPMLDERSQANPHLMRTAELMAAAQSLEDVVDVLAQTARRIAGSDGIAVVLREEDVCAYVAEDAIEPLWKGCRFPLDICVSGWAMQNRETAVILDVGSDPRIPVAAYAYKAIRSLVMVPIGAPEPVAALGAYWCAMVWLDTTTVSRLEALAHQATVALARLRAPQSAEPAIP
>NC_010172.1|WP_012254847.1|4007658_4008495_+|alpha/beta-hydrolase
MATITTGDGTEIFYKDWGPKDAQPIMFHHGWPLSSDDWDAQMLFFAHQGFRVVAHDRRGHGRSAQVSEGHDMDHYAADAAAVAQHLDLRNAVHIGHSTGGGEAARYVARHGEPQGRVAKAVLVSAVPPLMLKTEANPEGLPIEVFDGFRKALADNRAQFFLDVAAGPFYGFNRDGAAVREGVIRNWWRQGMMGSAKAHYEGIKAFSETDQTEDLRAISVPTLVLHGEDDQIVPIVAAARKSITLLRNGTLKTYPGLPHGMLTVNADQLNADILAFIRG
>NC_010172.1|WP_012254846.1|4006839_4007412_+|fasciclin-domain-containing-protein
MFRIRSSVTKSLTLGAALALSLAAAPTAALAKNPMVGGAPMYASKTIVENAVNSKDHTTLVAAVKAAGLVDTLSGPGPFTVFAPTDAAFAKLPPGTVDSLVQPQNKATLTGILTYHVVPGTYTAKDLMALAKRGGGEASLKTVQGEPLTVQARGKKVFVTDAKGNTATVTIANVMQSNGVIHVINGVLQP
>NC_010172.1|WP_012254845.1|4005795_4006716_-|SDR-family-oxidoreductase
MARHPWTAADIPSQAGRRAVVTGASAGLGFETARALAGAGAAVVLAVRDLEKGERAAAAIRREHPAAALSLRRIDTASLASVRAFAAEWPAEWAIDRLVLNAGIAAVPRREESVDGFERQLATNYLGHFALTGLLRPALSPSARVVSVSSLAHRSGRIRFDDLHWREIYGAQRAYRQSKLALLMFALELDRRLKAAGSGIASLAAHPGLARTEVFRRGDRAGAFQQGAGRAIFSLIGQPAAQGALPILYAATAPEAERGGYYGPDGVWEARGYPKPAAIASQALDRAAAGRLWAVSETLTGVRFAL
>NC_010172.1|WP_012254853.1|4012935_4014030_+|molybdenum-ABC-transporter-ATP-binding-protein
MTIAIDVSLRRDGFALDVAFSAGAGLTALFGRSGSGKTTVIDLIAGLAQPQRGRIAIDGTVLLDTARGIRVPVHRRRIGCVFQEARLMPHLSVRQNLRFGRLFSRGAGGPSLDAVADLLGIGPLLDRRPAGLSGGERQRVAIGRALLARPRLLLMDEPLSALDEARKREILPYIERLRDEAGLPIVYVSHSVAEVARLASTVVVLEAGRVAASGPVDAVLRRPDLIPDGAEAGSVLAMRVERHDPEAGLTRLTGPAGPLDLPLIDAPQGRRLNLRVPARDVLLALARPEALSARNVLPGRVAALREEGTACLVEVACGGATLAARLTRASARDLGLTVGCPVFAIVKSVALDAGEAPGGGRIEI
>NC_010172.1|WP_150105724.1|4014050_4014428_-|hypothetical-protein
MSVAVRTTPGGRGSSEEAIVAKIVVAAASRILRKLEGMVKGAEDEYAPTTLASRAARPSRGLDQPALSRNSQGYFVGARKIGAAQYRSPPSGSERQPDETGRVTLRIVVDVPLSLGATGCPVPMG
>NC_010172.1|WP_012254855.1|4014594_4015299_+|amino-acid-transporter
MTAPPGAPGSAHVGSGPSRDGSDGLVAASGTADRRNPSAPPEDDWSPWHPGELARRLETVARPWCVVGGWALDLWHGHQTRDHEDLEFTVLRDDVGVFRQALSDMTLYTVADGRFEILPEGAEPPPHILQIWCFDARAARWRVDLMIEPGEPDVWVCRRCPAIRHPRAEMVASTPDGIPYLRPAAVLLFKAKHRRDKDAADFERALPHLTHAERCWLREGLDRLHPGHAWAQAL
>NC_010172.1|WP_012254856.1|4015323_4016373_+|4-hydroxythreonine-4-phosphate-dehydrogenase-PdxA
MASDDRPLALTLGDPSGIGPEIALAAWQLRGERGVPPFQLIGDPEFLEATAYRLGLSVPVAEVEPDDACEVFPRALPVLPLPSGAKVIATPGAPDSANAGAIVESITAAVDLVRSGAASAVVTNPIAKFVLTRVGFAHPGHTEFLAALAAREGREPPLPVMMIWSDTLAVVPVTIHVALRRVPELLTQDLVERTARIVHADLRARFGLEHPRLVLSGLNPHAGESGTMGTEDRDVLAPAVAVLRSEGIDIRGPLPADTLFHERARATYDVALTPTHDQALIPVKTLAFDEGVNVTLGLPFVRTSPDHGTAFDIAGKGIAKPDSLIAALRLAHRLAHRPADTVLPFPVRA
>NC_010172.1|WP_012254857.1|4016381_4017272_+|16S-rRNA-(adenine(1518)-N(6)/adenine(1519)-N(6))--dimethyltransferase-RsmA
MSTEALSTDGLPPLREVVRRHGLEPKKALGQNFLFDLNLTGRIARSAGALEGVTVVEVGPGPGGLTRALLAAGAKRVVAIERDPRALPALAEIAAHYPGRLDVIDADAVGFDPRPLVGDGPVRIVANLPYNVATVLLTGWLGADTRDEAWPPWWESATLMFQREVAERIVADESDRANYGRLGVLCGWRTQATILFDVAPSAFVPPPKVTSSVVHLRPRPEPLPCRIADLERVTRAAFGQRRKMLRQSLKAATPDPIRLLTAAGLPETARAEEIPVVGFVAMARALEAGESRESGL
>NC_010172.1|WP_190274556.1|4017638_4017809_-|hypothetical-protein
MICAYIGPQYDFAAVPNGYTCPKCRRSIVSDDMACEIVGVSARCTACGKEMIVSPL
>NC_010172.1|WP_012254858.1|4017919_4018966_-|HlyD-family-efflux-transporter-periplasmic-adaptor-subunit
MNVRINRLDPHVPDPVESRRRAAGRVVRFAYAVGIFGVLGFFVVYFGAPFVFLSGPGIVSSPRHVLSLPFTVQVNRMNVRPGSAVKAGDEIGQVYSPEQETIVATYMRALSDIAGRSAELRVKARVAAESLEAARAYQRETETALERIAAMASTTTTFRVQILRERSLANKDVISQQAEAEEATKQLVSLDAFSKQIHEQLDEVKQNFRSGRVFALVGGIISTGLAQTGQSLVAGAPIADILNPNDVYVDWYIPNERLIDPRIGNDVFVLFGHRRIPGKITEILPVSALFGSSQQLTLRDRPATQIARIRFTSDASTPALNSTVCVHMHYSKISTDISRSLIKICGLD
>NC_010172.1|WP_012254859.1|4018962_4020234_-|glycosyltransferase-family-2-protein
MFAMLHVIVDTNWLLLAFSALMFDLPRYTFSLLSWSLVGIFRNSRPGDRWVEAAVSVIIPTFNGGSGLAPTIASLRQQTLSPLEIIIVDDGSTDDTRAVANRARQDGLVDMVICHGTRCGRSAAINAAARFASGDLIFTIDADTVLERDAIARLAHAFNDPRVAGACGNIAIRNESASLWTGLQSVEYMMSISAGKSILDVVGAIACLSGACSMYRRDVFARQGGLDVGPGEDLEFSLRLRRLGYRVRFVPDAWAETDGPVAAVGLLRQRARWDRDALRIRSMMYGELSFFHPLERLPDTLQRLDFIVFDLIPTMSLPFYSMYIVILFGPAHAILFIGAIYFLLLWISLFNMALAFVLFSRSPSFFSLGAAFIFPFYQGIYMKCARFVSYSSEILFATSRRDDYVPPRVRRALFADEVGKVVS
>NC_010172.1|WP_012254860.1|4020750_4021890_-|alpha/beta-hydrolase
MFPFDPSRQSRRRASRSLTRTTRQMTGQLTRSLESAAKSVTKSAAKSVTKAVTRSTEQAMREARKATIRSVKRTVRDTEKAVAAAVTRQGVKQGVEVARKPPGQFVTVDGLPVHVIVRGRGRPVVLVHGNGTMAEDFAICGLIDRLAARYRVIAIDRPGFGYSGRPRHRIWTAQAQAVLLHRVLEQLGVERPLIVGHSWGTIVALALAADGRRPLRGLVLLSGSYFPGHRTDAALIAPLAVPGLGDAMRAMVPAKVSASLTGQSFRQVFWPQPVPARFTARFSIEIAAAATQLRAVSEDAASMSSAVTGLQRRYAGLTLPVSVLAGDADAIVKASEHSVRLHTTIPGSTLRLLPGLGHMIHYGARLKVERAIDDLMKLR
>NC_010172.1|WP_012254861.1|4022089_4023595_-|SulP-family-inorganic-anion-transporter
MSSTVSTARGRFGSTWFANWFANPWADILSGIVVALALIPEAIGFSVIAGVDPKVGLYASVVIACVIAFAGGRPAMISAATAATAVVMVDLVRDHGVQYLFAATILMGVFQILAGLARLGRLMRFVSRSVMTGFVNALAILIFLAQLPELTGVTPATYGLIALGLAIIYGFPKLTRAVPSPLVAIAVLTALTAYLGLDVRTVAHLGALPTTLPSFALPDVPLTFETLRIILPYSATLAAVGLLESLLTAQIVDDMTDTGSSKNRECMGQGLANMSSAVFGGMGGCAMIGQSVINVSSGARGRLSTLVAGSFLLALLVLLQDLLAIVPVAALTAVMIMVSLNTFSWRSLLALRTNPLPSSLVMLATVAVVVATRDLAIGVLVGVLLSGVFFAGKVARMSRITAELSLDGRTRTYRVAGQVFFASAGSFAEAIDVREPVERLVIDVHAAHFWDISAVGALDRVVMKARAAGRTVEVVGLNEASATLVERFGQHDKADASLPAH

Crispr_ID: NC_010172_5

• CRISPR_ID: NC_010172_5
• CRISPR_location: 4640799-4640887
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 1 spacers

Consensus repeat of NC_010172_5

Consensus_repeat	Method
CGCCGGCTTTCGGAAAAAGATGATGC	CRISPRCasFinder

spacers of NC_010172_5

>5.1|4640825|37|NC_010172|CRISPRCasFinder
AAGAACGAAAGTGAAGAGCGTCATCCCGAAAGGTGGC

CRISPR arrays and Neighbor proteins around NC_010172_5

CRISPR arrays

The CRISPR arrays of NC_010172_5

>merge|NC_010172|5|4640799-4640887|CRISPRCasFinder
CGCCGGCTTTCGGAAAAAGATGATGCAAGAACGAAAGTGAAGAGCGTCATCCCGAAAGGTGGCCGCCGGCTTTCGGAAAAAGATGATGC

>NC_010172|5|5|4640799-4640887|CRISPRCasFinder
CGCCGGCTTTCGGAAAAAGATGATGC	AAGAACGAAAGTGAAGAGCGTCATCCCGAAAGGTGGC
CGCCGGCTTTCGGAAAAAGATGATGC

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
NC_010172.1|WP_012255304.1|4646536_4647004_+|DUF2721-domain-containing-protein	unknown	unknown	gnl|CDD|378544
NC_010172.1|WP_150105734.1|4628922_4629159_-|hypothetical-protein	unknown	unknown	unknown
NC_010172.1|WP_012255296.1|4636961_4637159_+|hypothetical-protein	unknown	unknown	unknown
NC_010172.1|WP_003599084.1|4636414_4636783_+|hypothetical-protein	unknown	unknown	unknown
NC_010172.1|WP_012255305.1|4647004_4648516_-|NAD(P)/FAD-dependent-oxidoreductase	unknown	unknown	gnl|CDD|224983
NC_010172.1|WP_012255306.1|4648512_4649448_-|alpha/beta-hydrolase	unknown	unknown	gnl|CDD|369561
NC_010172.1|WP_003599086.1|4636102_4636312_+|cold-shock-protein	unknown	unknown	gnl|CDD|224197
NC_010172.1|WP_012255307.1|4649450_4650275_-|SDR-family-NAD(P)-dependent-oxidoreductase	unknown	unknown	gnl|CDD|187632
NC_010172.1|WP_012255293.1|4629228_4631418_-|ATP-dependent-DNA-helicase-RecG	unknown	unknown	gnl|CDD|236794
NC_010172.1|WP_003599068.1|4643164_4643359_-|hypothetical-protein	unknown	unknown	unknown
NC_010172.1|WP_003599081.1|4637225_4637567_-|hypothetical-protein	unknown	unknown	unknown
NC_010172.1|WP_012255295.1|4632135_4635726_+|transcription-repair-coupling-factor	unknown	unknown	gnl|CDD|224118
NC_010172.1|WP_012255302.1|4643608_4645417_+|glycosyltransferase-family-39-protein	unknown	unknown	gnl|CDD|224720
NC_010172.1|WP_003599063.1|4646191_4646521_+|lipid-A-disaccharide-synthase-N-terminal-domain-containing-protein	unknown	unknown	gnl|CDD|226461
NC_010172.1|WP_012255298.1|4637825_4639226_-|acyl--CoA-ligase	unknown	unknown	gnl|CDD|223395
NC_010172.1|WP_042509004.1|4639523_4640777_+|ATP-binding-protein	unknown	unknown	gnl|CDD|237425
NC_010172.1|WP_012255300.1|4640976_4641339_+|response-regulator	unknown	unknown	gnl|CDD|381145
NC_010172.1|WP_012255294.1|4631609_4631906_+|succinate-dehydrogenase-assembly-factor-2	unknown	unknown	gnl|CDD|225489
NC_010172.1|WP_012255303.1|4645413_4646187_+|glycosyltransferase-family-2-protein	unknown	unknown	gnl|CDD|133022
NC_010172.1|WP_012255301.1|4641394_4643026_-|acyl-CoA-synthetase	unknown	unknown	gnl|CDD|236169

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
NC_010172.1|WP_012255304.1|4646536_4647004_+|DUF2721-domain-containing-protein	gnl|CDD|378544	pfam11026, DUF2721, Protein of unknown function (DUF2721). This family is conserved in bacteria. The function is not known.	5.69889e-22
NC_010172.1|WP_042509004.1|4639523_4640777_+|ATP-binding-protein	gnl|CDD|237425	PRK13557, PRK13557, histidine kinase; Provisional.	5.22379e-87
NC_010172.1|WP_003599086.1|4636102_4636312_+|cold-shock-protein	gnl|CDD|224197	COG1278, CspC, Cold shock proteins [Transcription].	4.06042e-32
NC_010172.1|WP_012255307.1|4649450_4650275_-|SDR-family-NAD(P)-dependent-oxidoreductase	gnl|CDD|187632	cd05374, 17beta-HSD-like_SDR_c, 17beta hydroxysteroid dehydrogenase-like, classical (c) SDRs. 17beta-hydroxysteroid dehydrogenases are a group of isozymes that catalyze activation and inactivation of estrogen and androgens. SDRs are a functionally diverse family of oxidoreductases that have a single domain with a structurally conserved Rossmann fold (alpha/beta folding pattern with a central beta-sheet), an NAD(P)(H)-binding region, and a structurally diverse C-terminal region. Classical SDRs are typically about 250 residues long, while extended SDRs are approximately 350 residues. Sequence identity between different SDR enzymes are typically in the 15-30% range, but the enzymes share the Rossmann fold NAD-binding motif and characteristic NAD-binding and catalytic sequence patterns. These enzymes catalyze a wide range of activities including the metabolism of steroids, cofactors, carbohydrates, lipids, aromatic compounds, and amino acids, and act in redox sensing. Classical SDRs have an TGXXX[AG]XG cofactor binding motif and a YXXXK active site motif, with the Tyr residue of the active site motif serving as a critical catalytic residue (Tyr-151, human 15-hydroxyprostaglandin dehydrogenase (15-PGDH) numbering). In addition to the Tyr and Lys, there is often an upstream Ser (Ser-138, 15-PGDH numbering) and/or an Asn (Asn-107, 15-PGDH numbering) contributing to the active site; while substrate binding is in the C-terminal region, which determines specificity. The standard reaction mechanism is a 4-pro-S hydride transfer and proton relay involving the conserved Tyr and Lys, a water molecule stabilized by Asn, and nicotinamide. Extended SDRs have additional elements in the C-terminal region, and typically have a TGXXGXXG cofactor binding motif. Complex (multidomain) SDRs such as ketoreductase domains of fatty acid synthase have a GGXGXXG NAD(P)-binding motif and an altered active site motif (YXXXN). Fungal type ketoacyl reductases have a TGXXXGX(1-2)G NAD(P)-binding motif. Some atypical SDRs have lost catalytic activity and/or have an unusual NAD(P)-binding motif and missing or unusual active site residues. Reactions catalyzed within the SDR family include isomerization, decarboxylation, epimerization, C=N bond reduction, dehydratase activity, dehalogenation, Enoyl-CoA reduction, and carbonyl-alcohol oxidoreduction.	9.23626e-61
NC_010172.1|WP_012255293.1|4629228_4631418_-|ATP-dependent-DNA-helicase-RecG	gnl|CDD|236794	PRK10917, PRK10917, ATP-dependent DNA helicase RecG; Provisional.	0
NC_010172.1|WP_012255306.1|4648512_4649448_-|alpha/beta-hydrolase	gnl|CDD|369561	pfam07859, Abhydrolase_3, alpha/beta hydrolase fold. This catalytic domain is found in a very wide range of enzymes.	1.28002e-57
NC_010172.1|WP_012255303.1|4645413_4646187_+|glycosyltransferase-family-2-protein	gnl|CDD|133022	cd04179, DPM_DPG-synthase_like, DPM_DPG-synthase_like is a member of the Glycosyltransferase 2 superfamily. DPM1 is the catalytic subunit of eukaryotic dolichol-phosphate mannose (DPM) synthase. DPM synthase is required for synthesis of the glycosylphosphatidylinositol (GPI) anchor, N-glycan precursor, protein O-mannose, and C-mannose. In higher eukaryotes,the enzyme has three subunits, DPM1, DPM2 and DPM3. DPM is synthesized from dolichol phosphate and GDP-Man on the cytosolic surface of the ER membrane by DPM synthase and then is flipped onto the luminal side and used as a donor substrate. In lower eukaryotes, such as Saccharomyces cerevisiae and Trypanosoma brucei, DPM synthase consists of a single component (Dpm1p and TbDpm1, respectively) that possesses one predicted transmembrane region near the C terminus for anchoring to the ER membrane. In contrast, the Dpm1 homologues of higher eukaryotes, namely fission yeast, fungi, and animals, have no transmembrane region, suggesting the existence of adapter molecules for membrane anchoring. This family also includes bacteria and archaea DPM1_like enzymes. However, the enzyme structure and mechanism of function are not well understood. The UDP-glucose:dolichyl-phosphate glucosyltransferase (DPG_synthase) is a transmembrane-bound enzyme of the endoplasmic reticulum involved in protein N-linked glycosylation. This enzyme catalyzes the transfer of glucose from UDP-glucose to dolichyl phosphate. This protein family belongs to Glycosyltransferase 2 superfamily.	2.88185e-47
NC_010172.1|WP_012255302.1|4643608_4645417_+|glycosyltransferase-family-39-protein	gnl|CDD|224720	COG1807, ArnT, 4-amino-4-deoxy-L-arabinose transferase and related glycosyltransferases of PMT family [Cell envelope biogenesis, outer membrane].	7.85551e-45
NC_010172.1|WP_003599063.1|4646191_4646521_+|lipid-A-disaccharide-synthase-N-terminal-domain-containing-protein	gnl|CDD|226461	COG3952, COG3952, Predicted membrane protein [Function unknown].	5.18556e-27
NC_010172.1|WP_012255298.1|4637825_4639226_-|acyl--CoA-ligase	gnl|CDD|223395	COG0318, CaiC, Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II [Lipid metabolism / Secondary metabolites biosynthesis, transport, and catabolism].	4.63894e-21
NC_010172.1|WP_012255295.1|4632135_4635726_+|transcription-repair-coupling-factor	gnl|CDD|224118	COG1197, Mfd, Transcription-repair coupling factor (superfamily II helicase) [DNA replication, recombination, and repair / Transcription].	0
NC_010172.1|WP_012255300.1|4640976_4641339_+|response-regulator	gnl|CDD|381145	cd18161, REC_hyHK_blue-like, phosphoacceptor receiver (REC) domain of hybrid sensor histidine kinase/response regulators similar to Pseudomonas savastanoi blue-light-activated histidine kinase. Typically, two-component regulatory systems (TCSs) consist of a sensor (histidine kinase) that responds to specific input(s) by modifying the output of a cognate response regulator (RR). TCSs allow organisms to sense and respond to changes in environmental conditions. Hybrid sensor histidine kinase (HK)/response regulators contain all the elements of a classical TCS in a single polypeptide chain. Pseudomonas savastanoi blue-light-activated histidine kinase is a photosensitive HK and RR that is involved in increased bacterial virulence upon exposure to light. RRs share the common phosphoacceptor REC domain and different effector/output domains such as DNA, RNA, ligand-binding, protein-binding, or enzymatic domains. REC domains function as phosphorylation-mediated switches within response regulators, but some also transfer phosphoryl groups in multistep phosphorelays.	3.86603e-21
NC_010172.1|WP_012255294.1|4631609_4631906_+|succinate-dehydrogenase-assembly-factor-2	gnl|CDD|225489	COG2938, COG2938, Uncharacterized conserved protein [Function unknown].	3.85398e-27
NC_010172.1|WP_012255305.1|4647004_4648516_-|NAD(P)/FAD-dependent-oxidoreductase	gnl|CDD|224983	COG2072, TrkA, Predicted flavoprotein involved in K+ transport [Inorganic ion transport and metabolism].	6.43693e-94
NC_010172.1|WP_012255301.1|4641394_4643026_-|acyl-CoA-synthetase	gnl|CDD|236169	PRK08162, PRK08162, acyl-CoA synthetase; Validated.	0

Protein sequences

>NC_010172.1|WP_042509004.1|4639523_4640777_+|ATP-binding-protein
MLELLRRLWEVENLSPHGICLLWRPELIWTHVVSDSLIAFAYFSIPIGLAYFVSRRRDVVFGWMFWSFAVFITACGATHLMAIWTLWVPDYGLEAAVKLITGMASIGTAVALWVLIPKALTLPSPTQLRQANEALQARIAERDAALAALEAANAERARAEEALRQSQKMEAIGQLTGGVAHDFNNLLNVVLLNLDRVERGLPEASPLRTRLRDAVKGAERAATMTHKLLAFARRQPLSPVSTDVNAQIASFAEFLRGTIGPRIRLETDLAPNLPRVRIDPNQFESAILNLAVNARDAMPEGGTLTLATRPLPEGDGFAVEVSDTGAGMAPEVEARAFEPFFTTKPLGQGTGLGLSQVFGFAQQSGGSAAFVRDGRPGTTVRLSFPAQAPAMSRPAARPAPVAEPGFAPLLPPVAVAG
>NC_010172.1|WP_012255298.1|4637825_4639226_-|acyl--CoA-ligase
MSAQRAITQVEASQRALQGALKGASQGTPDAASHETPADAPLRRISLGSLLSATARRYPDRIAVVDPADKPDWSDRPAITWTYAAAAEIVERLARGLRSWRLPPGSRIGLCLPGSAESALAILAVEAAGHVACLLPVSWDEDRLLAAAQNVALSAVLTQARLGSAAPAERLCAVAARYFGLRYLAAFGPDVPDGVINLDRFVLDGPAGEPAGPAPAAAGIVSFIGGDPEQPVYRSGESVVAAAAAHLVAMRVAPAERILSLIGPHDLRGLATGLAAALVAGATLETLPLFDGAAFATALRRPGPTHLVAPAFLEKNLAGRDLPTDLRSVALVHRAPVRFPSRSRAVDRPQGLRLETVIDTVAFDETAILSGRCGTTGDLSLVLGKPERLALPTALLSMRRGADDRLAFRGQACTVTPLQRGNRQAIPSGMWQETPYAPVLFAGFATAIEVVEPSDSAGSPKIFAPA
>NC_010172.1|WP_003599081.1|4637225_4637567_-|hypothetical-protein
MGALQQVLVVDDGRRAVDRSLAADLAGLGYASVTASIEAADDVLAVIARPAAILLQLSSSTNPHLAALADRLRARMRAGGIPVIEMDDAGPRPGAPVDLKSRIGPYVLAEPEL
>NC_010172.1|WP_012255296.1|4636961_4637159_+|hypothetical-protein
MFHKFKIGQRVRRAQSSPTDERLGFGTISEVVRLMPDDPTGEPSYRIRSGSAERAVRESEIIAAA
>NC_010172.1|WP_003599084.1|4636414_4636783_+|hypothetical-protein
MSFTKQNSFDDRRQDSASSREAMLARFRARPSNDDPAVQARQAERRAIAVARDERVKERETQRRLDAERLAAIAAAEREAEVARKAAEIEAAAERAREAQAKQKEERDARYAARKAKIKLRR
>NC_010172.1|WP_003599086.1|4636102_4636312_+|cold-shock-protein
MSIGTVKWFNEQKGFGFIQPEDGSKDVFVHISAVERAGLRGLTEGQKVSYEMETDRRSGKQSAGQLQVA
>NC_010172.1|WP_012255295.1|4632135_4635726_+|transcription-repair-coupling-factor
MAKPQPKPPVPKPQTARFALPKSAPLTKALDAIKRGDSPTLASVPDGFDALVAADLARALSGSFEGPAVLVHVARDSGRSNAFQNALKFVAPEIEAMSVPAWDCQPYDRVSPNGAIAAQRMTALSRLTRSRSSEEKPRILCTTVNALVQRVPPRSRVAVETFSAAIGNVVPMDKVVAWVEANGFLRTGTVRDTGEYAVRGGILDLSPPGLPNPIRLDFFGDTLESIRAFDPETQRTVGSLRSLDLVPMSEVQLTTETIRRFRQGYLSSFGAATRDDRLYETVSEGRRYAGLEHWMPLFYDGLDTLFDYLGDVPMLFDPQVEEAATERLALIQDYFQAREAALKNPQSGVAPYKPLPPRALYLTPNEWKSKVESGTVVRLTPFATPDSDERAVIDCGARAGRSFAPERADDAASVFDAAVAHIRDLQASGHHVILGSWSEGSRDRLCGVLTDHGLKKPTVINTFAAVNALKRGTDVAVAVWGLESGFTLDRLAVVAEGDILGDRLVRQKRKSKRPQDIILEVQALQPGDLVVHADHGIGRFVTLKTVTAAGAPHDCLELQYAGGLLLLPVENIELLTRYGSEDSEVALDRLGGGAWQARKAKMKRRILEMAGDLIKIAAARFVKSAPSLKAPEGLYEEFAARFAFQETEDQANAIDAVLDDLNAGRPMDRLVCGDVGFGKTEVALRAAFAAAISGKQVAVVVPTTLLARQHFRTFAERFKGLPINVAQLSRFVSAGDQRQNRAGLADGTVDIVVGTHALLAKNVAFKDLGLIIVDEEQHFGVAHKERLKALQADVHVLTLSATPIPRTLQLAMTGVRELSIIATPPMDRLAVRTFVTPFDPLLVREALLRERYRGGQSFYVAPRIEDLAEVKRFLDTEMPETTVAVAHGQMAAGQLEDVMTAFYEGKYDVLLSTTIVESGLDIPTANTLIVHRADMFGLAALYQLRGRVGRSKARAYALFTTPANRQLTTQAEQRLKVLQSLDTLGAGFQLASHDLDIRGAGNLLGDAQSGHIKEVGYELYQQMLEDAVTALKAGIEEPVEEAWSPTIALGAPVTIPEDYVEDLTVRLGLYRRLSTLENDAEMESFGAELIDRFGPLPPEVEQLLKIVTIKILCRDANVEKVEAGPKGVVVHFRDKSFANPQGLAAMVVEQGSFAKVRPDMSVVFIRELDGIPRRLKETTQILRGLVTIAKRAKKAA
>NC_010172.1|WP_012255294.1|4631609_4631906_+|succinate-dehydrogenase-assembly-factor-2
MSGTTRTSADLDPRRRRTLFRAWHRGIREMDLIMGRFADSEIGALSEEELTQFEALIEVPDRDLFRWLTGEDATPENYDTAVYRRLKAFHRHDAPIHS
>NC_010172.1|WP_012255293.1|4629228_4631418_-|ATP-dependent-DNA-helicase-RecG
MTSDTSQTLGHPADAAPPRSGGDVAPGLRPSLLDPLFAPARGLPGVGPKIAPLIEKLLGSEERPARVADLLFHLPQSGVARPLCGSIRDAPTGEPVTLGVTVVAHRPPMLGKRAFRVLVEDGTGDISLVFFGMPRGRIEKMLPLGSHRYVSGRIDLWDGTRQMVHPSRILDQEGLASLPAVEPIYGATEGLTSRVISRIVHGALDRLPVLPEWQDASYLARNRFPPFAEALRIEHRPEEAPPPPVEGQAPPASPARKRLAYDELLASQLALALVRARQRRKPGRVNAGDGHLKERVEAALPFALTGAQARAVEEIRADMAAERRMLRLLQGDVGSGKTAVALLAMASAVEAGRQAALMAPTEILARQHYERLVPLAGAMRLRLLTGRDRAAERRATLADLAAGEIDIVVGTHALFQESVAFRDLGLAVVDEQHRFGVHQRLALGAKGEAVDILVMTATPIPRTLALTFFGDMDVSVLDEKPAGRQPIKTITVPVERIDEVVGGLHRALAAGDRVYWICPLVAESEYVDLAAAAERFEDLQKHFGDAVGLIHGKMPGPEKDAAMEAFAAGRTRVLVSTTVVEVGVDVPEATIMVIEHAERFGLAQLHQLRGRVGRGSKASSCLLLYRGPLGQVAKARLEMMRGSEDGFRIAEEDLRLRGEGEVLGTRQSGAAAFRLARLETDGDLLEAARDDARLIVERDPRLQSERGRALRVLLYLFEREAAIRLIGAG
>NC_010172.1|WP_150105734.1|4628922_4629159_-|hypothetical-protein
MIARSETRSETDDPERVVQEAEAAAPDIPGCGNAAREKSVFRFQSSLCAASLALGTSVGQGSKGGITLYLPQLPLNWR
>NC_010172.1|WP_012255300.1|4640976_4641339_+|response-regulator
MADKRTVLVVEDEEMLLSAVTMEFEDAGFAVLSAGTAEEAYGLLQGAGRVDLLFTDIRLPGQLDGWDLAERARVLHPALPVIYVTGYSAEQPRQVSESVLVMKPYRMTAIMSAAQQLGVE
>NC_010172.1|WP_012255301.1|4641394_4643026_-|acyl-CoA-synthetase
MARTTIYDRDLDPNPANFQPLTPLTYLDRAARTFPDRVAVIHGPLRRSYADLYARCRRLAAALAARGIGRGDTVAVLLANTPAMIECHYGVPMTGAVLNTLNTRLDAGALAFCLDHGEAKVFIVDREFARIGREALDKAGVSPLVIDYDDPEFTGDSAPVGETDYEDVLAAGDPDFDWAMPSDEWDAISLNYTSGTTGDPKGVVYHHRGAALLSLGNVITAGLPQHAVYLWTLPMFHCNGWCFPWTLSIVAGTHVCLRQVRAPAMYAALAEHGVTHLSGAPIVMSTLLNAPEAQKRPLPRRVHFLTAAAPPPEAVLAAMGEAGFDVTHLYGLTETYGPAVVNAWHEDWDALSRDEQARKKARQGVRYPVLEGLDVRDPETMESLPADGTSLGEVMFRGNVVMRGYLKNPASTEAAFKGGWFRSGDLGVKHPDGYIQLKDRSKDIIISGGENISSIEVEDALFKHPAVAAAAVVAKPDAKWGETPCAFVELKEGREATSEELVAWCRERLAPYKLPRHVVFGELPKTSTGKVQKFVLREKAREE
>NC_010172.1|WP_003599068.1|4643164_4643359_-|hypothetical-protein
MANNSANPYTLEIEACERPAGHFTWAIRRNGKLFQRADRVQTTEEAAERSGLAALEKLLSGRER
>NC_010172.1|WP_012255302.1|4643608_4645417_+|glycosyltransferase-family-39-protein
MTRLGVAPAALSRAEGGWPLDGALRLGDRVLSFGAASHLRACLLLLLIGLASFLPGLASLQPMDRDEPRFAQASKQMLETGDLVDIRFQAEARHKKPVGIYWAQAAVVAAGEALGVPQARTQIGLYRIPSLLGALAAILLTYWAGLALLDRRRALLAAALFSACIMLSAEARLAKTDALLTACSVAAFGALARAWLGRARLERRRGPASLGTALVFWLGLALGILVKGPMVPLFAGLAVFVLCLREGSARWLLDLRPRLGLLITLAVVAPWFLAIAWKSGGAFFGEAVGRDMLGKVGTGAEKHWGPPGAYALAFFATFWPGAAFAALSLPFAWARRGEEAVALLLAWIVPMWLIFEAVPTKLPHYVLPLMPAVAILTVLALSRGALDPRRPGARWVAGLVGLIPVGLTLGLSLAAWRLDHVLPLAALPLLLAACLLAGLAWAAFARGAREGAGQGAGQGTRQEAGEGALVLAVAASVVLSGAVFGLTQPVLQSLKVSPRLAAIRDALPCEAPRVASLGLREPSLVFTVGTDLAMLNSGAEAIAFLREGGCRLVLVEDRFAAEFTAAEGGQPLTPIGRVTGFNINGGKPVGVSAYAALPGSTP
>NC_010172.1|WP_012255303.1|4645413_4646187_+|glycosyltransferase-family-2-protein
MSTTTQSVDPVFRPPRLSVVVPVKNEAGNIEALVAEIGHACQTLAPFEVIYVDDGSTDATLPTLRGLQAKHLWLRVVRHDRGGGQSGAVRSGVLRARAPVIATLDGDGQNDPSFIPVLYAALEQAGPTVGLAQGQRIGRKGAWKQFQSWIANGVRGKILKDATRDTGCGLKVFRREVYLTLPYFDALHRFMPALVTREGFDVVHRDVVDRPRFTGHSNYGMFDRLWVGILDLAGVWWLIRRRRPAPRAEEIPHSESR
>NC_010172.1|WP_003599063.1|4646191_4646521_+|lipid-A-disaccharide-synthase-N-terminal-domain-containing-protein
MLIQLAEDLPAYFYDVFVTRFDFWLVFGIGAQLVFGSRFILQWIASERAGKSVMPLSFWFLSIIGGLMTLVYGFVRREPVIIIGQGLSTFIYLRNLALIFRERRRARRA
>NC_010172.1|WP_012255304.1|4646536_4647004_+|DUF2721-domain-containing-protein
MFQTATGDPESIAHIIQVALAPAFLLSALATLLNVFSTRLGRVADKVDALSASLPGADEAARATLARRLAYLRRRSLLLDLAVVLASLGGVMTGAAVLTLFVGALRDASAASVLFACFGLALGCTILAILAFLAEILLAGRGIRLEVERKQDRPR
>NC_010172.1|WP_012255305.1|4647004_4648516_-|NAD(P)/FAD-dependent-oxidoreductase
MSEAPLDLVIVGAGFSGLAMAIRARQAGFDRLLVLEKAAGVGGTWRENTYPGAACDVPSQLYSLSFAPKSDWSRLFAPQPEIEAYLEGLVAAHGLAPFLRLETCVTRAVFDAEAGLWRVETDRGVVSARVLVGAMGALHHPALPGLPGLERFSGKAFHSAAWDHSCDLTAQRVGVIGTGASAVQFVPEIVGRVAHLTQFQRSAPWILPRGDRPTGRGFRRLMRIAPLRRLHRASLFWRREVAALAGFTRVSRVTALAEAWSRRHLAASIADPALRRQLTPDYRLGCKRVLLSDDYYPALAQPHVSLVTAPIREVLPHGIVTADGAEHPLDVLIFATGFAPGGSVVRTEVIGRDGLSLLQAWKQGADAFRGVSVAGFPNFFLLLGPNTGLGHNSVLLMVEAQVAHVLGALKRLRDQPGGVLEVRPEAQARFREEVDARMRDSIWTQGGCGSWYLDRAGRNRTLWPGTVGDYVRGTERMRVEDYRLTDPPGLCPGPTKGLVLSKP
>NC_010172.1|WP_012255306.1|4648512_4649448_-|alpha/beta-hydrolase
MASLRAHLYDLTIRLAVKGRLGGTHDVYAIRRAFEGARFPTPAGVAFTPGEVGGVPGEWAEAAGAKTEGTRAEGEHPTLLYCHGGGFIACSAQLYRPVTGRFARAGFRVFTPDYRLAPEHPFPAAVEDCTGVWATLAEAGPAVVAGDSAGGSLALTTIIEARRLGLTPPVAAALFSPATDLIGRSASFRTNGRRDAMFRPEALMRLVPAYLAGADPADPRASPIEADFSGFPPLLIHVGVREILRDDSLRLAEKAKAAGVPVELTVFPVVAHAWQLAESFLPEARRSLDEAALFLHRALKAPPHPEAEVAA
>NC_010172.1|WP_012255307.1|4649450_4650275_-|SDR-family-NAD(P)-dependent-oxidoreductase
MAQRFVLRGRTALVTGAASGIGAALSLALAGRGCALALADRDVQGLAETARAARAAGVTVSEHVLDLADREALLALPEAVRARHGGLHLLVNNAGVALGGRFEETDPADIDWLMDVNLHAVMRLTHACLPLLRAQAQAQIVNLSSVFGIIAPAGQAAYAASKFAVRGFTEALRHEVEGTGLGVTLVHPGGVATAIARNARRPRAMDPHEAAEAHLAFERLLTMSPAKAAAAILRGIETRAPRVVIGADARRALLIQRLMPVRYWSLIRRAVRDR

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity
NC_010172_4	4.1|4012831|39|NC_010172|CRISPRCasFinder	4012831-4012869	39	NC_010172.1	2717865-2717903	2	0.949
NC_010172_4	4.1|4012831|39|NC_010172|CRISPRCasFinder	4012831-4012869	39	NC_010172.1	4640786-4640824	2	0.949
NC_010172_4	4.1|4012831|39|NC_010172|CRISPRCasFinder	4012831-4012869	39	NC_010172.1	5229931-5229969	2	0.949

1. spacer 4.1|4012831|39|NC_010172|CRISPRCasFinder matches to position: 2717865-2717903, mismatch: 2, identity: 0.949

cccgaaaggtgggcaccggctttcggaaaaagatgatgc	CRISPR spacer
cccgaaaggtggtctccggctttcggaaaaagatgatgc	Protospacer
************ * ************************

2. spacer 4.1|4012831|39|NC_010172|CRISPRCasFinder matches to position: 4640786-4640824, mismatch: 2, identity: 0.949

cccgaaaggtgggcaccggctttcggaaaaagatgatgc	CRISPR spacer
cccgaaaggtggtcgccggctttcggaaaaagatgatgc	Protospacer
************ *.************************

3. spacer 4.1|4012831|39|NC_010172|CRISPRCasFinder matches to position: 5229931-5229969, mismatch: 2, identity: 0.949

cccgaaaggtgggcaccggctttcggaaaaagatgatgc	CRISPR spacer
cccgaaaggtggcctccggctttcggaaaaagatgatgc	Protospacer
************ * ************************

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity
NC_010172_3	3.2|2700418|25|NC_010172|CRISPRCasFinder	2700418-2700442	25	NC_015314	Pseudonocardia dioxanivorans CB1190 plasmid pPSED01, complete sequence	87393-87417	4	0.84
NC_010172_3	3.1|2700364|31|NC_010172|CRISPRCasFinder	2700364-2700394	31	NZ_CP010408	Streptomyces vietnamensis strain GIMV4.0001 plasmid pSVL1, complete sequence	46469-46499	5	0.839
NC_010172_3	3.1|2700364|31|NC_010172|CRISPRCasFinder	2700364-2700394	31	NZ_LR134451	Tsukamurella tyrosinosolvens strain NCTC13231 plasmid 9, complete sequence	745-775	5	0.839
NC_010172_3	3.2|2700418|25|NC_010172|CRISPRCasFinder	2700418-2700442	25	NC_010683	Ralstonia pickettii 12J plasmid pRPIC01, complete sequence	37260-37284	5	0.8
NC_010172_3	3.1|2700364|31|NC_010172|CRISPRCasFinder	2700364-2700394	31	NZ_CP017422	Arthrobacter sp. ZXY-2 plasmid pZXY21, complete sequence	256369-256399	6	0.806
NC_010172_3	3.1|2700364|31|NC_010172|CRISPRCasFinder	2700364-2700394	31	NZ_CP039697	Novosphingobium sp. ABRDHK2 plasmid pABRDHK22, complete sequence	681520-681550	6	0.806
NC_010172_3	3.1|2700364|31|NC_010172|CRISPRCasFinder	2700364-2700394	31	NZ_CP023549	Rhodobacter sp. CZR27 plasmid unnamed1, complete sequence	62521-62551	6	0.806
NC_010172_3	3.1|2700364|31|NC_010172|CRISPRCasFinder	2700364-2700394	31	NZ_CP048420	Sphingomonas insulae strain KCTC 12872 plasmid unnamed2, complete sequence	70285-70315	6	0.806
NC_010172_3	3.1|2700364|31|NC_010172|CRISPRCasFinder	2700364-2700394	31	NC_006569	Ruegeria pomeroyi DSS-3 megaplasmid, complete sequence	30928-30958	6	0.806
NC_010172_3	3.1|2700364|31|NC_010172|CRISPRCasFinder	2700364-2700394	31	NZ_CP018784	Curtobacterium pusillum strain AA3 plasmid pCPAA3, complete sequence	498426-498456	8	0.742
NC_010172_3	3.1|2700364|31|NC_010172|CRISPRCasFinder	2700364-2700394	31	CP007644	Rhizobium etli bv. phaseoli str. IE4803 plasmid pRetIE4803c, complete sequence	21554-21584	8	0.742
NC_010172_3	3.1|2700364|31|NC_010172|CRISPRCasFinder	2700364-2700394	31	NZ_CP050095	Rhizobium leguminosarum bv. trifolii strain 22B plasmid pRL22b1, complete sequence	109422-109452	8	0.742
NC_010172_3	3.1|2700364|31|NC_010172|CRISPRCasFinder	2700364-2700394	31	NZ_CP050083	Rhizobium leguminosarum bv. trifolii strain 31B plasmid pRL31b3, complete sequence	165370-165400	8	0.742
NC_010172_3	3.1|2700364|31|NC_010172|CRISPRCasFinder	2700364-2700394	31	NZ_CP050100	Rhizobium leguminosarum bv. trifolii strain 9B plasmid pRL9b3, complete sequence	101792-101822	8	0.742
NC_010172_3	3.1|2700364|31|NC_010172|CRISPRCasFinder	2700364-2700394	31	NC_008381	Rhizobium leguminosarum bv. viciae 3841 plasmid pRL10, complete sequence	306491-306521	8	0.742
NC_010172_3	3.1|2700364|31|NC_010172|CRISPRCasFinder	2700364-2700394	31	NZ_CP025017	Rhizobium leguminosarum strain Norway plasmid pRLN5, complete sequence	97123-97153	8	0.742
NC_010172_3	3.1|2700364|31|NC_010172|CRISPRCasFinder	2700364-2700394	31	NZ_CP049733	Rhizobium leguminosarum strain A1 plasmid pRL10, complete sequence	156629-156659	8	0.742
NC_010172_3	3.1|2700364|31|NC_010172|CRISPRCasFinder	2700364-2700394	31	NZ_CP053443	Rhizobium leguminosarum bv. trifolii strain CC275e plasmid pRltCC275eC, complete sequence	77895-77925	8	0.742
NC_010172_3	3.1|2700364|31|NC_010172|CRISPRCasFinder	2700364-2700394	31	NZ_CP048284	Rhizobium leguminosarum bv. viciae 248 plasmid pRle248b, complete sequence	98593-98623	8	0.742
NC_010172_3	3.1|2700364|31|NC_010172|CRISPRCasFinder	2700364-2700394	31	NZ_CP054026	Rhizobium sp. JKLM12A2 plasmid pPR12A205, complete sequence	129091-129121	8	0.742
NC_010172_3	3.1|2700364|31|NC_010172|CRISPRCasFinder	2700364-2700394	31	NZ_CP054036	Rhizobium sp. JKLM13E plasmid pPR13E05, complete sequence	255135-255165	8	0.742
NC_010172_3	3.1|2700364|31|NC_010172|CRISPRCasFinder	2700364-2700394	31	NZ_CP053207	Rhizobium leguminosarum bv. trifolii TA1 plasmid pRltTA1C, complete sequence	187281-187311	8	0.742
NC_010172_3	3.1|2700364|31|NC_010172|CRISPRCasFinder	2700364-2700394	31	NZ_CP050106	Rhizobium leguminosarum bv. trifolii strain 4B plasmid pRL4b5, complete sequence	359536-359566	8	0.742
NC_010172_3	3.1|2700364|31|NC_010172|CRISPRCasFinder	2700364-2700394	31	NC_012854	Rhizobium leguminosarum bv. trifolii WSM1325 plasmid pR132505, complete sequence	227486-227516	8	0.742
NC_010172_3	3.1|2700364|31|NC_010172|CRISPRCasFinder	2700364-2700394	31	NZ_CP018232	Rhizobium leguminosarum strain Vaf-108 plasmid unnamed4, complete sequence	218220-218250	8	0.742
NC_010172_3	3.1|2700364|31|NC_010172|CRISPRCasFinder	2700364-2700394	31	NZ_CP025506	Rhizobium leguminosarum bv. viciae strain UPM791 plasmid pRlvE, complete sequence	350341-350371	8	0.742
NC_010172_3	3.1|2700364|31|NC_010172|CRISPRCasFinder	2700364-2700394	31	NZ_CP050111	Rhizobium leguminosarum bv. trifolii strain 3B plasmid pRL3b1, complete sequence	359720-359750	8	0.742
NC_010172_3	3.1|2700364|31|NC_010172|CRISPRCasFinder	2700364-2700394	31	NZ_CP030763	Rhizobium leguminosarum strain ATCC 14479 plasmid unnamed3, complete sequence	22889-22919	8	0.742
NC_010172_3	3.1|2700364|31|NC_010172|CRISPRCasFinder	2700364-2700394	31	NZ_CP022668	Rhizobium leguminosarum bv. viciae strain BIHB 1217 plasmid pPR3, complete sequence	238448-238478	8	0.742
NC_010172_3	3.1|2700364|31|NC_010172|CRISPRCasFinder	2700364-2700394	31	NZ_CP022568	Rhizobium leguminosarum bv. viciae strain BIHB 1148 plasmid pSK04, complete sequence	289251-289281	8	0.742
NC_010172_3	3.1|2700364|31|NC_010172|CRISPRCasFinder	2700364-2700394	31	NZ_CP050088	Rhizobium leguminosarum bv. trifolii strain 23B plasmid pRL23b6, complete sequence	496042-496072	8	0.742
NC_010172_3	3.1|2700364|31|NC_010172|CRISPRCasFinder	2700364-2700394	31	NZ_CP028969	Aminobacter sp. MSH1 plasmid pUSP1, complete sequence	272474-272504	9	0.71
NC_010172_3	3.1|2700364|31|NC_010172|CRISPRCasFinder	2700364-2700394	31	NZ_CP026266	Aminobacter sp. MSH1 plasmid pAM01, complete sequence	269751-269781	9	0.71
NC_010172_3	3.1|2700364|31|NC_010172|CRISPRCasFinder	2700364-2700394	31	CP047389	Agrobacterium sp. CGMCC 11546 plasmid pA	170400-170430	9	0.71
NC_010172_3	3.1|2700364|31|NC_010172|CRISPRCasFinder	2700364-2700394	31	NZ_CP015007	Aminobacter aminovorans strain KCTC 2477 plasmid pAA02, complete sequence	276449-276479	9	0.71

1. spacer 3.2|2700418|25|NC_010172|CRISPRCasFinder matches to NC_015314 (Pseudonocardia dioxanivorans CB1190 plasmid pPSED01, complete sequence) position: , mismatch: 4, identity: 0.84

tagcggccaagccgatttccaggcc	CRISPR spacer
gcgcggcccagccgatgtccaggcc	Protospacer
  ****** ******* ********

2. spacer 3.1|2700364|31|NC_010172|CRISPRCasFinder matches to NZ_CP010408 (Streptomyces vietnamensis strain GIMV4.0001 plasmid pSVL1, complete sequence) position: , mismatch: 5, identity: 0.839

cacgggccgcgacacgatcaccatcggcgac	CRISPR spacer
catccgccgcgacacgatcgccttcggcgac	Protospacer
**.  **************.** ********

3. spacer 3.1|2700364|31|NC_010172|CRISPRCasFinder matches to NZ_LR134451 (Tsukamurella tyrosinosolvens strain NCTC13231 plasmid 9, complete sequence) position: , mismatch: 5, identity: 0.839

-cacgggccgcgacacgatcaccatcggcgac	CRISPR spacer
gcac-caccgcgacccgatccccatcggcgac	Protospacer
 ***  .******* ***** ***********

4. spacer 3.2|2700418|25|NC_010172|CRISPRCasFinder matches to NC_010683 (Ralstonia pickettii 12J plasmid pRPIC01, complete sequence) position: , mismatch: 5, identity: 0.8

tagcggccaagccgatttccaggcc	CRISPR spacer
tcgcggccaagccgatttccattgg	Protospacer
* *******************    

5. spacer 3.1|2700364|31|NC_010172|CRISPRCasFinder matches to NZ_CP017422 (Arthrobacter sp. ZXY-2 plasmid pZXY21, complete sequence) position: , mismatch: 6, identity: 0.806

cacgggccgcgacacgatcaccatcggcgac	CRISPR spacer
ctacgacggcgacacgatcaccatcggcaac	Protospacer
*   *.* ********************.**

6. spacer 3.1|2700364|31|NC_010172|CRISPRCasFinder matches to NZ_CP039697 (Novosphingobium sp. ABRDHK2 plasmid pABRDHK22, complete sequence) position: , mismatch: 6, identity: 0.806

-cacgggccgcgacacgatcaccatcggcgac	CRISPR spacer
cgacaagac-cggcacgatcaccatcggcgac	Protospacer
  **..* * **.*******************

7. spacer 3.1|2700364|31|NC_010172|CRISPRCasFinder matches to NZ_CP023549 (Rhodobacter sp. CZR27 plasmid unnamed1, complete sequence) position: , mismatch: 6, identity: 0.806

cacgggccgcgacacgatcaccatcggcgac	CRISPR spacer
cacggtccgcgacacgatcaccgacttcgtc	Protospacer
***** ****************. *  ** *

8. spacer 3.1|2700364|31|NC_010172|CRISPRCasFinder matches to NZ_CP048420 (Sphingomonas insulae strain KCTC 12872 plasmid unnamed2, complete sequence) position: , mismatch: 6, identity: 0.806

-cacgggccgcgacacgatcaccatcggcgac	CRISPR spacer
cgacaagac-cggcacgatcaccatcggcgac	Protospacer
  **..* * **.*******************

9. spacer 3.1|2700364|31|NC_010172|CRISPRCasFinder matches to NC_006569 (Ruegeria pomeroyi DSS-3 megaplasmid, complete sequence) position: , mismatch: 6, identity: 0.806

cacgggccgcgacacgatcaccatcggcgac	CRISPR spacer
caagctctgcggcatgatcaccatcggcgac	Protospacer
** *  *.***.**.****************

10. spacer 3.1|2700364|31|NC_010172|CRISPRCasFinder matches to NZ_CP018784 (Curtobacterium pusillum strain AA3 plasmid pCPAA3, complete sequence) position: , mismatch: 8, identity: 0.742

cacgggccgcgacacgatcaccatcggcgac	CRISPR spacer
cggcaaacgcgacaccgtcaccatcggcgac	Protospacer
*.  .. ******** .**************

11. spacer 3.1|2700364|31|NC_010172|CRISPRCasFinder matches to CP007644 (Rhizobium etli bv. phaseoli str. IE4803 plasmid pRetIE4803c, complete sequence) position: , mismatch: 8, identity: 0.742

cacgggccgcgacacgatcaccatcggcgac	CRISPR spacer
ctatcccggcgtcacgatcaccatccgcgac	Protospacer
*     * *** ************* *****

12. spacer 3.1|2700364|31|NC_010172|CRISPRCasFinder matches to NZ_CP050095 (Rhizobium leguminosarum bv. trifolii strain 22B plasmid pRL22b1, complete sequence) position: , mismatch: 8, identity: 0.742

cacgggccgcgacacgatcaccatcggcgac	CRISPR spacer
cccccttggcgacacgatcgccgtcggcgac	Protospacer
* *   . ***********.**.********

13. spacer 3.1|2700364|31|NC_010172|CRISPRCasFinder matches to NZ_CP050083 (Rhizobium leguminosarum bv. trifolii strain 31B plasmid pRL31b3, complete sequence) position: , mismatch: 8, identity: 0.742

cacgggccgcgacacgatcaccatcggcgac	CRISPR spacer
cccccttggcgacacgatcgccttcggcgac	Protospacer
* *   . ***********.** ********

14. spacer 3.1|2700364|31|NC_010172|CRISPRCasFinder matches to NZ_CP050100 (Rhizobium leguminosarum bv. trifolii strain 9B plasmid pRL9b3, complete sequence) position: , mismatch: 8, identity: 0.742

cacgggccgcgacacgatcaccatcggcgac	CRISPR spacer
cgcccctggcgacacgatcgccttcggcgac	Protospacer
*.*   . ***********.** ********

15. spacer 3.1|2700364|31|NC_010172|CRISPRCasFinder matches to NC_008381 (Rhizobium leguminosarum bv. viciae 3841 plasmid pRL10, complete sequence) position: , mismatch: 8, identity: 0.742

cacgggccgcgacacgatcaccatcggcgac	CRISPR spacer
cccccttggcgacacgatcgccgtcggcgac	Protospacer
* *   . ***********.**.********

16. spacer 3.1|2700364|31|NC_010172|CRISPRCasFinder matches to NZ_CP025017 (Rhizobium leguminosarum strain Norway plasmid pRLN5, complete sequence) position: , mismatch: 8, identity: 0.742

cacgggccgcgacacgatcaccatcggcgac	CRISPR spacer
cccccttggcgacacgatcgccctcggcgac	Protospacer
* *   . ***********.** ********

17. spacer 3.1|2700364|31|NC_010172|CRISPRCasFinder matches to NZ_CP049733 (Rhizobium leguminosarum strain A1 plasmid pRL10, complete sequence) position: , mismatch: 8, identity: 0.742

cacgggccgcgacacgatcaccatcggcgac	CRISPR spacer
cccccttggcgacacgatcgccttcggcgac	Protospacer
* *   . ***********.** ********

18. spacer 3.1|2700364|31|NC_010172|CRISPRCasFinder matches to NZ_CP053443 (Rhizobium leguminosarum bv. trifolii strain CC275e plasmid pRltCC275eC, complete sequence) position: , mismatch: 8, identity: 0.742

cacgggccgcgacacgatcaccatcggcgac	CRISPR spacer
cgcccctggcgacacgatcgccttcggcgac	Protospacer
*.*   . ***********.** ********

19. spacer 3.1|2700364|31|NC_010172|CRISPRCasFinder matches to NZ_CP048284 (Rhizobium leguminosarum bv. viciae 248 plasmid pRle248b, complete sequence) position: , mismatch: 8, identity: 0.742

cacgggccgcgacacgatcaccatcggcgac	CRISPR spacer
cccccttggcgacacgatcgccgtcggcgac	Protospacer
* *   . ***********.**.********

20. spacer 3.1|2700364|31|NC_010172|CRISPRCasFinder matches to NZ_CP054026 (Rhizobium sp. JKLM12A2 plasmid pPR12A205, complete sequence) position: , mismatch: 8, identity: 0.742

cacgggccgcgacacgatcaccatcggcgac	CRISPR spacer
cccccttggcgacacgatcgccgtcggcgac	Protospacer
* *   . ***********.**.********

21. spacer 3.1|2700364|31|NC_010172|CRISPRCasFinder matches to NZ_CP054036 (Rhizobium sp. JKLM13E plasmid pPR13E05, complete sequence) position: , mismatch: 8, identity: 0.742

cacgggccgcgacacgatcaccatcggcgac	CRISPR spacer
cccccttggcgacacgatcgccgtcggcgac	Protospacer
* *   . ***********.**.********

22. spacer 3.1|2700364|31|NC_010172|CRISPRCasFinder matches to NZ_CP053207 (Rhizobium leguminosarum bv. trifolii TA1 plasmid pRltTA1C, complete sequence) position: , mismatch: 8, identity: 0.742

cacgggccgcgacacgatcaccatcggcgac	CRISPR spacer
cccccttggcgacacgatcgccttcggcgac	Protospacer
* *   . ***********.** ********

23. spacer 3.1|2700364|31|NC_010172|CRISPRCasFinder matches to NZ_CP050106 (Rhizobium leguminosarum bv. trifolii strain 4B plasmid pRL4b5, complete sequence) position: , mismatch: 8, identity: 0.742

cacgggccgcgacacgatcaccatcggcgac	CRISPR spacer
cccccttggcgacacgatcgccttcggcgac	Protospacer
* *   . ***********.** ********

24. spacer 3.1|2700364|31|NC_010172|CRISPRCasFinder matches to NC_012854 (Rhizobium leguminosarum bv. trifolii WSM1325 plasmid pR132505, complete sequence) position: , mismatch: 8, identity: 0.742

cacgggccgcgacacgatcaccatcggcgac	CRISPR spacer
cgcccttggcgacacgatcgccgtcggcgac	Protospacer
*.*   . ***********.**.********

25. spacer 3.1|2700364|31|NC_010172|CRISPRCasFinder matches to NZ_CP018232 (Rhizobium leguminosarum strain Vaf-108 plasmid unnamed4, complete sequence) position: , mismatch: 8, identity: 0.742

cacgggccgcgacacgatcaccatcggcgac	CRISPR spacer
cccccttggcgacacgatcgccttcggcgac	Protospacer
* *   . ***********.** ********

26. spacer 3.1|2700364|31|NC_010172|CRISPRCasFinder matches to NZ_CP025506 (Rhizobium leguminosarum bv. viciae strain UPM791 plasmid pRlvE, complete sequence) position: , mismatch: 8, identity: 0.742

cacgggccgcgacacgatcaccatcggcgac	CRISPR spacer
cccccttggcgacacgatcgccttcggcgac	Protospacer
* *   . ***********.** ********

27. spacer 3.1|2700364|31|NC_010172|CRISPRCasFinder matches to NZ_CP050111 (Rhizobium leguminosarum bv. trifolii strain 3B plasmid pRL3b1, complete sequence) position: , mismatch: 8, identity: 0.742

cacgggccgcgacacgatcaccatcggcgac	CRISPR spacer
cccccttggcgacacgatcgccttcggcgac	Protospacer
* *   . ***********.** ********

28. spacer 3.1|2700364|31|NC_010172|CRISPRCasFinder matches to NZ_CP030763 (Rhizobium leguminosarum strain ATCC 14479 plasmid unnamed3, complete sequence) position: , mismatch: 8, identity: 0.742

cacgggccgcgacacgatcaccatcggcgac	CRISPR spacer
cccccttggcgacacgatcgccttcggcgac	Protospacer
* *   . ***********.** ********

29. spacer 3.1|2700364|31|NC_010172|CRISPRCasFinder matches to NZ_CP022668 (Rhizobium leguminosarum bv. viciae strain BIHB 1217 plasmid pPR3, complete sequence) position: , mismatch: 8, identity: 0.742

cacgggccgcgacacgatcaccatcggcgac	CRISPR spacer
cccccttggcgacacgatcgccttcggcgac	Protospacer
* *   . ***********.** ********

30. spacer 3.1|2700364|31|NC_010172|CRISPRCasFinder matches to NZ_CP022568 (Rhizobium leguminosarum bv. viciae strain BIHB 1148 plasmid pSK04, complete sequence) position: , mismatch: 8, identity: 0.742

cacgggccgcgacacgatcaccatcggcgac	CRISPR spacer
cccccttggcgacacgatcgccttcggcgac	Protospacer
* *   . ***********.** ********

31. spacer 3.1|2700364|31|NC_010172|CRISPRCasFinder matches to NZ_CP050088 (Rhizobium leguminosarum bv. trifolii strain 23B plasmid pRL23b6, complete sequence) position: , mismatch: 8, identity: 0.742

cacgggccgcgacacgatcaccatcggcgac	CRISPR spacer
cccccttggcgacacgatcgccttcggcgac	Protospacer
* *   . ***********.** ********

32. spacer 3.1|2700364|31|NC_010172|CRISPRCasFinder matches to NZ_CP028969 (Aminobacter sp. MSH1 plasmid pUSP1, complete sequence) position: , mismatch: 9, identity: 0.71

cacgggccgcgacacgatcaccatcggcgac	CRISPR spacer
ggcaagccgcgatccgatcaccatcggctca	Protospacer
 .*..*******. **************   

33. spacer 3.1|2700364|31|NC_010172|CRISPRCasFinder matches to NZ_CP026266 (Aminobacter sp. MSH1 plasmid pAM01, complete sequence) position: , mismatch: 9, identity: 0.71

cacgggccgcgacacgatcaccatcggcgac	CRISPR spacer
ggcaagccgcgatccgatcaccatcggctca	Protospacer
 .*..*******. **************   

34. spacer 3.1|2700364|31|NC_010172|CRISPRCasFinder matches to CP047389 (Agrobacterium sp. CGMCC 11546 plasmid pA) position: , mismatch: 9, identity: 0.71

cacgggccgcgacacgatcaccatcggcgac	CRISPR spacer
agtcgatcgcaacgcgatcaccatcggcgag	Protospacer
 .. *..***.**.**************** 

35. spacer 3.1|2700364|31|NC_010172|CRISPRCasFinder matches to NZ_CP015007 (Aminobacter aminovorans strain KCTC 2477 plasmid pAA02, complete sequence) position: , mismatch: 9, identity: 0.71

cacgggccgcgacacgatcaccatcggcgac	CRISPR spacer
gttgagccgcgccacgatcgccatcggcagt	Protospacer
  .*.****** *******.********...

Prophage detection

Region	Region Position	Protein_number	Hit_taxonomy	Key_proteins	Att_site
DBSCAN-SWA_1	168919 : 173047	6	Acidovorax_phage(33.33%)	NA	NA
DBSCAN-SWA_2	1589389 : 1600923	14	Paracoccus_phage(28.57%)	protease,head,tail	NA

Sequences of DBSCAN-SWA_1

Nucleotide sequences of DBSCAN-SWA_1

>NC_010172|168919:173047|DBSCAN-SWA
CATGGACACCCCGAACGCACGACCAAAGCGTGCGACCGATCTGGACCGTCTTGTTGGAGAGCGCATCCAGGTGCTGCGCAAGTCCAAGGGTCTTACGCAGACCGCGCTCGGTCAGGCCATCGGCGTCACGTTCCAGCAGGTCCAGAAGTACGAGAACGGCATGAACCGCGTCGGCGCTAGCCGTCTGAGCGACATCGCTCGCGTGCTCGAAGTGCGAGTCTCGTCCTTCTACGATGGCGACGAGGGCGAGGGCGAGGACCGAGCCGAGGTGTTTGGTTTTCTACGCACTCACGGTGCCATCGATCTCCTGCGCGCCTTCAGCGAGATCGAGGATGATCAACTGCGCCGCGAGGTTCTGGCGATTGTGCGCACGGTTGCTCGCGTCGAACAAGTGCGGAACAGCTGATTCACTTTCATGCCGGCTCCCGCTGGACCATTCGACCGCGCGGCAGGTTTACGAGCCGGCCCTCTGGGAAGCCGCAGCGGGCGGCGCCAGCGATAACGACCGCAAGGCCGAGGTGCGAAAGCCGATGCTCGCGCCGCCATTTGATTTTTAACTTATGATTGCCCAAACTAAATACATTGCTTAGGGGCTGTTGGCGGCGATCGGGTCGCAATCAAGTCGCCCCGCCACTCGCAATGTAGAGCATGAACCCATGGACGATCATAGTGCATCGCCGAAGCAGGCGACAAAGGAGGACGTTATCGTCGGCCTGCGTATCCAGACTTTGCGCAAGTCGAGGGGCATCTCACAAACTGCGCTCGGCATTGCCATCGGCGTGTCGTTCCAGCAGATCCAGAAATATGAGATTGGCGCCAACCGCGTCGGCGCCGGTCGCCTGAGCGACATCGCTCGGGTACTTGGAGCGCCGGTCTCCGTCTTCTTTGAGGAAGGGGATGCCGCCGCGGCGCAGGAGAAAACAGAGGTCTTCGATTTGCTGCGTGCCCCAGGCGCCGTCGATCTGCTGAACGCCTTCATCACGATCGAGGATGACCGGCTACGCCGCGAGGTTCTGGCCCTCGTTCGAAGCGCGGCTCGCATGGAGCAAGATCACGGCGCCTGAGCCTGAGGCACCGACAATTGCTGAATTGAGCGAACGCTAAGACGACCGCATGGCCAAGGTGCGGAAGCGGATGATTGCGGTTGCACACCTTGAGCGAGTAGAAGATCTCTTGGCGCAGGCACTCGTCACGAAGCTTCTGCTCGGAGACATCCGCCTCCTTGCACGCCAACGCTAAACTCTTGCCCTGAGCTGTCTGTACCTCGATCTTGCGCAGCTTCAGCACCACCTGCTCCGCGCTCGTCTTCTGTCCTCGTCGCATGTCCGACTCCTTCGGTCCTAGCTGATCCTCTCAGTCAGCCCGGTCCAAAACCAGCCGGTCAGGTCACGGCGCAGCCATACGTAACCAAGCAGGTGCTCGCCGAGCTTGGCAACGCGTGCATCCAAGGTCCGCTCGTCCACCAAAAGCGCACCTTCCGCCTCTCACAAGCGAAGGTCCGTTGATGGCGCGAAGCCGAAGCCGAGACGCGCGGCATCCCCCTATGCCAGCACCTTGGCTGCGTTGGCACCATGCTCGCGGACATGAACCTCAGCGAGGCGGATACGTGGGCTGAGGTTTTGCTGGGCGAGCCAACCCCTGACCCAGCTGTAGATGTGCTCGGCGAATGCCTCGCAGCTGACGTGAGGGAGAACGACGAGTTGGATCAAGCCGACCTCGTCCATCGCCCGCATCTCGGACAGCAGCGGATCGTCCGCAGCTACCAGCGTCTTGTGATCGAAGGTTTGCTCCAGCCACTGCCTCACTGGCTTGAGGCTGCCGAAGTCCATGACCCAACTGCGAGCATCGAGGTCATCAGCCTCAAACTCCAAGTGCACGGCGAGAGCGTAGCCGTGGAGAAATCGGCAGTGCGATGGCGACCGCCACTGCCGGAAGCAGCAGGACAGGCCGATATCGTGGCCATAGGTCTTCGTGCTTCGAAACCTCACCCTTGCATCCCCTTTCGCATGATGCTGGGACTTGCGCACCGTTAGGCCAAGGAGTGCGTCGTTCGCCGCCGTGCGGTCTGAAATCGGCCGGCGGCGTGACTGGCTGAATGAGAGGCGCCACGGACGCCTAGAGCTTCTCGAACACCTCGTTGTACAGCGCCTCACCCGAAAAGAACGGGGTGAGCTGCTGCCGGCAGGGCTCCAGATCGACGCCGGCCTCAGCACGAGCCAGCACCCCACGGAGGTGGTCCACCAGCTGGGGTTTGTGCCGAAGGTAATCCTCCCAGCTGGCGGTCCATTCCGATGGGTACCGGAACTCCTCCGGGTACATCTCGGTGTAGCTGAGCCGATCCGGCACCACCGGGACGGCACCTGACAGCACGCCCTCATAGCAGCCGATGCCAAGGGTCTCTTGCAGCGAGCCCGAGAACACCACCCGAGCCTCAGCTAGGCGACGGTGGTAGGTATCCTTGGAGAGGGGTGTTCGCTGGCAGGCATCGAATTGCCAGTCCGGGAACGCATTGGCGAGATCGTCGAAGATCTCTACCTGCTTCTCAGGTGCGGGAACTCGGTGGGGAAACAGCACGAGGTTGCGCTTGGGCATGCCCCTGTAGGGTTCCAGCAAGCGGGGCAGATACTCCATGGGCCAACCCACCTTCACGACGCGGCCGCCGTCTTCGGCCGCGCCAGGGCCCTTACGGAACAGGTCGATGTGCGCTTGGGTGGCGAAGGCATTCACGTCGTAGGCCTTGAACAGCGCCCGCTCCATGTCCTTTGCCCAAGGCACGTGCTGGGGTCCGATCCGACCTAGGAAGTCGTTGGGATCGTAATGCCCGGCATGCCAAAGGCCCACGATGCGCACTGGGATCGGGAGCAGCGCGCTCATATAGCGAAGCTGATGCACGCCAGGGTGCCAAGCGTCGGTGAACACGAACACATCGTTGGGTTTCACCTCACCGCGCTGGAAGCGGCGAGCCAACTCGGCAACCTGCGTGCTCTTGAAAATGTTGGTGGCGGCGAAGTCGAGGAACGCGCCTTTGGTGGGGATTTGATCCGGCACGTCGCCGTCGATGTCGATCACTCGCACATTCACTACGCAATCTTCCGCAGCGTGTTCGATCTGCTGCCGCAGGTGATGCCGCAGCTGCCCGGTATAGCGGGTCTCCACACTCTCGATGTGGAGCAGCCAGACGTTCTTCACCTCGCCCCCCGCTCATAGCGCATGAGGGCACCACACTCGCCATCTTCGTCCACCTCGATCCAGATGTCTCGGTTCTGGAAGCGCTGTTCCTCGACCATACGCGCATACAGTTCGTTGGCCAGCATCTCGCAACTTTTGCCGTCAGCCTCCAGAACACCGTCCTTGGCATACAGGCTCTCGAGCCAAGCCTGCGTCATGTGGAACTCGATCTCGCGATTGTCTCCGAACACCTCGATCCACACACGGAAGCCAAACTGATGCCGATGGCGCTCGCGCAGATATGCAACCGCTTCGGGCGCACCCGGATAACAGTGGAAGCCACGTACTTTGAACGTGACCCAGATCATACGATTGGGCGACACGCCCGCGAACATTGCAGCAGAAGTCATCGCCACTCAGCAGGTTTCAAGGAATGCGAGCGGATGGCACGTCGGCTGAGCCAGAGCCACATGAGTATGCTGGCCACCATCTTGACTGTGTAGTGCACCCAAAACAGTTGTTGATCCCAGAAGTGCCAAGGGTCGAGCCAGCTCATTATGCCGAGGAAGATCAAGCCGTCGAACGGCACTGCCACAGCTGACGAAATAACGATGCGGTCCTTAATGGGGCGCTTCGTTATTTTGAAGATGCCAAAGTCGATGCTCTCGCTCACGAAGAAGGCGGCGCCACTGGCGAACGCCACGACGTGATCCACGGCGAAATACGTCAGCACTGAAGCGAGTAGCGTGAGGGGGACGACCCAATTGCCAACGGCACGCTGCGCATAATCGCGCATCACGAAGATGGCGCCTACCAGCAGCAAGCCCGGTGGTATCTCGATGCCGAGAACCTTCTGTGGTGGCCACCATGAAATTGTGAAATTGACCAGCAGCGCCACTGCCACATAAGCGAGTGCGATCAGCGCAGCTTTTAACTGCCGGCTCGTTCTGTTCCATGTCAT

Protein sequences of DBSCAN-SWA_1

>NC_010172|168919:173047|169574_169982_+|WP_012251976.1|DBSCAN-SWA
MDDHSASPKQATKEDVIVGLRIQTLRKSRGISQTALGIAIGVSFQQIQKYEIGANRVGAGRLSDIARVLGAPVSVFFEEGDAAAAQEKTEVFDLLRAPGAVDLLNAFITIEDDRLRREVLALVRSAARMEQDHGA
>NC_010172|168919:173047|168919_169324_+|WP_012251975.1|DBSCAN-SWA
MDTPNARPKRATDLDRLVGERIQVLRKSKGLTQTALGQAIGVTFQQVQKYENGMNRVGASRLSDIARVLEVRVSSFYDGDEGEGEDRAEVFGFLRTHGAIDLLRAFSEIEDDQLRREVLAIVRTVARVEQVRNS
>NC_010172|168919:173047|170459_170906_-|WP_042508625.1|DBSCAN-SWA
MRFRSTKTYGHDIGLSCCFRQWRSPSHCRFLHGYALAVHLEFEADDLDARSWVMDFGSLKPVRQWLEQTFDHKTLVAADDPLLSEMRAMDEVGLIQLVVLPHVSCEAFAEHIYSWVRGWLAQQNLSPRIRLAEVHVREHGANAAKVLA
>NC_010172|168919:173047|172492_173047_-|WP_012251980.1|DBSCAN-SWA
MTWNRTSRQLKAALIALAYVAVALLVNFTISWWPPQKVLGIEIPPGLLLVGAIFVMRDYAQRAVGNWVVPLTLLASVLTYFAVDHVVAFASGAAFFVSESIDFGIFKITKRPIKDRIVISSAVAVPFDGLIFLGIMSWLDPWHFWDQQLFWVHYTVKMVASILMWLWLSRRAIRSHSLKPAEWR
>NC_010172|168919:173047|172103_172502_-|WP_150105673.1|DBSCAN-SWA
MAMTSAAMFAGVSPNRMIWVTFKVRGFHCYPGAPEAVAYLRERHRHQFGFRVWIEVFGDNREIEFHMTQAWLESLYAKDGVLEADGKSCEMLANELYARMVEEQRFQNRDIWIEVDEDGECGALMRYERGAR
>NC_010172|168919:173047|171033_172107_-|WP_012251978.1|DBSCAN-SWA
MKNVWLLHIESVETRYTGQLRHHLRQQIEHAAEDCVVNVRVIDIDGDVPDQIPTKGAFLDFAATNIFKSTQVAELARRFQRGEVKPNDVFVFTDAWHPGVHQLRYMSALLPIPVRIVGLWHAGHYDPNDFLGRIGPQHVPWAKDMERALFKAYDVNAFATQAHIDLFRKGPGAAEDGGRVVKVGWPMEYLPRLLEPYRGMPKRNLVLFPHRVPAPEKQVEIFDDLANAFPDWQFDACQRTPLSKDTYHRRLAEARVVFSGSLQETLGIGCYEGVLSGAVPVVPDRLSYTEMYPEEFRYPSEWTASWEDYLRHKPQLVDHLRGVLARAEAGVDLEPCRQQLTPFFSGEALYNEVFEKL

Homologous phage analysis in the prophage region

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_012251975.1|168919_169324_+	helix-turn-helix transcriptional regulator	Q8W6G2	Sinorhizobium_phage	7.0e-19	43.5
WP_012251976.1|169574_169982_+	helix-turn-helix transcriptional regulator	A0A223W054	Agrobacterium_phage	1.5e-16	39.2
WP_042508625.1|170459_170906_-	6-carboxytetrahydropterin synthase	L7TKZ6	Rhizobium_phage	2.1e-45	61.4
WP_012251978.1|171033_172107_-	hypothetical protein	A0A218M2Y2	Acidovorax_phage	1.5e-44	35.6
WP_150105673.1|172103_172502_-	hypothetical protein	A0A218M2Y7	Acidovorax_phage	6.2e-20	47.5
WP_012251980.1|172492_173047_-	hypothetical protein	A0A2I7S8X9	Vibrio_phage	4.1e-22	41.1

Sequences of DBSCAN-SWA_2

Nucleotide sequences of DBSCAN-SWA_2

>NC_010172|1589389:1600923|DBSCAN-SWA
GTCAGCGGCCGGCGCGCAGCCAGTCCCACAGGCGCGCGCGCCAGCCGGGCCGCGCGCTGGACGGCGAGGATGGGCGTTCGACGGGGGCGGCGACGGGTTGCGCATCGCTGCGTCGGGCGCGCGGCAGGGCGGTTTCGTAGGGCGTGTGGAACTGGTCGAACTCGCCCTGTCGACGGGGGATGATCGCGGCCGGGCGGTTCCACATCAGGATCGCTTCGCCCGCGCCCGCCCGGTCGCCGGCCCGCAGGCGCCGCAGCACCGTGGAGCGCCGGAACGCCGCCGGGCCGATGTTGAAGCAAAGCGAGACCAGCGCGTCGAAGGCGTGCTGCGGCAGCGGCTCGGGGATCGCCTCGGCGACGGTGCGGACGAAGGCCGCCACGTCGCGGGTGAAGATCGCGTCCGCCTCCCCGGCCTCGATGCGCAGGCCCGCCCGCGGCACGGGCGGCCCGGCGGCGGCGGTGTGGCCGATGCCGATGGTCCAGACCCCCACGCTGTCGCGGTAGGCTTCCAGCCGTCGCCCCTCCCGGGCGATCAGCACGGCGCGGCCGACGGCACTGAGGTCCATCGTCAATCTCCGGTCTTGAGGGAATGGGTCGAGAGGCGGGCTCGGCGGCCGACGGCTCTAGGTCATTGTTTTGCCGCGCCTTCTTTCGACGAACCGGTCGCCACTTCGTCGGAATGCGCTCTAATCCAAGCCCTCGAACAGCAGCGAGAACAGGATGTTGGTGGTGGGCTGCGCCCCGCTGCTCGGCACGATCTTGAGGCACCAGCGGTCCGCCGCCTCGAAGACGGCGCTGTTGACGAGGTCGGCCGCTTGGTTGGAACCGGCGCCGGCGATCGTGCAGGTCAGGGCCGTGCCGGTGAACAGGTTCTGGAGCGTGACGGTCCAGCTCTGACCGGCGCCCGGCGCGCCCTGCGTCACGACCCGCAAATCGCGGAACCGCCCCCGCCGGCCGGCGGCGGCGTAGACCACGCTGGGGTGGCCGCCGACGAGGGCGTTGGTGAAGTACCGCGTCGCCCCGGCCGGGATGGAGCCCGGCCCGCTGTTGCCGGCGATCACCTGCGCCGTCAGAACCGGCACGTCCGCGACGGTGATCCGACCGGGAAAGGCGACCCGCCCCGTCTCGCGCCCGATCCGCAGCGCCTCGAACCAGTCGCCGCCATTCGGGGAGACCTTCACACGAAGGTCGTCGTCACCGGTCAGGCCGATCTCGGCGCGGGCGGAGAACCCGGTCTGCATCAGCAGGGAGAGGGTGTTGCCGGTCGCCTGCTTGTTCAGGGTGTAGCGCAGATCGCCGCTTCCGCCCTCCGCGACCGTCAGCGCGGTCCAGAGCGCCTTGTTGAGCTTGGCCGCGAAGGGATTGTCTACATCCGCCCCGGTGCCGAGCCCGAGTCGGGTCAGATTGTCCAGGGCGGTGATGGTCGCGCGGAACGGCATCCAGGCCGAGCCAGTGAAAGTGTAGAGTTCGGCCTCGTCGGCGACGTAGGCCGTCCAGCCCACAAGCGGCTTGAGGGACAGCCACGCACCGTCCTGGAAGCGGGCGATGCGCCCGGCCCAGCCGGTCCAGGCCCCGCTCGGGTTGCCCGACACGATCAGGTAGCGGTCGCCCTCGGCGGGGCTCGCGGGTGGGGCGGCCCGATCCTTGTCGAGGACGGCGAGCTGCACCAGCGCGTCAAGCAGCGCCAGGGCCTCGTTATGGGTGACATGCTTCTGCGCCTGCCCCCCCGCGATCAGCGGCAGGGCAAGGCTCGGGGTCGTATCGGCCATGGGCCACTCCGGAAATGTCAGGTGTCAGACGGTCCGCAGGCTCACCCGTGTCCGGCGGGCGGGGCCGGGGCCGGCGAGCGAGCCGATCGGCACCAATGCGGCGTCGATCTCGGTGCGGGGCGTGCCGAAATCGGCGATCTCGTCGGCGCGTGCGTAGAGCGCGCTCGTCGTGGCGGTGCTAAGGCTGCGCAGAATCCGCCCGTCCTCGGCGAGGATGTCGAGGCGGTAGGCCTCCGCCCCCGCGCCCGGCGGCAGGTCGGCCGGCTCCCAGGCGTCGGCATCGCGCCGCGCCCGCCGGATCCAATGGAAGCGGATGCCCTCGGCCTCGCGCCGGGCGCGCAGATGGACCGGGCTTAAGGGGGTGAAGGCGGTCAGGTCGGCCGTTGCCGCGAAACCGACGAAGGTGGGATCGGCCGGATCGCGCTCCGCCGGGCCGACCCGGTAGAGAAAGCGGCGGCCGACCTCGTCGAGCCGATCGACGAGGGGCCGGACGGCGCCGTCGTCGAGGCGCACGATCAGGCTGCCCGTGCCCGCCGAACGGCCGGCGGCGGCCTCGCTGCCGGCCAAGCCTCGCAGCAGGCTCGAGAGCCGCCACGTGCCGGCCCCGATCAGCTCGGCCTTGGCCGCAGACAGGAGTTCGACCGTCCCATCCGCGCCGACCAGAGCGAACAGGTTGCCGCCGGCCAGCGCGGCAGCCTCGCCGATCGCGCTCAACCCTTCCGCGTGGCGCAGGGTGACGTCGAGGCGGGCGTTGCGGTCGAAGCGCCAGAGCGGGCCGGGCGGCAGCGGGGTCAGCGTGCGGCCGAGGCAGGCCGGATGGTCGAGGGTGCGGTGGAGCGCGAACGGCGCGCCCTCGCCGGCCGAGCGCCAGACCGCGACGGCACCGGGCCAGGGATCGGCCGAGACCGCGAGATAGGCCAGCGGCACCGGGTCGCCGCGCTCCACCGGCAGAACGAGCGGCAGGGCGAAGACCGGCCCCGGCACCGCCGGCGGCCTCCGGCCCGTTCTCGGGGCCGGCGTGGTGGGTGCGCGATCGGACCGAGGCAACGGCACGGCCCGGGTCTCGATCCGGCGGCCCGTGGGCGCGTCGTCGATCCGCACGATGCGGTGAAGCGTCCCGCTATCCGCGTCCGGGAGCGCCACCACATCGCCGGGCTCCAGATCGATCCGGCGCGGGCTCACCCCGAAAGCGGCCGTATCCCGGCCCGCGAGCGCCGCGTCGAGGACGGCCTCCGCCAGCGCCTGCACGGCGTCGCGCCGGGTGACGATGGCCGCGTCAACGCGCACCTCCTGCCGCCGCGCGCCGGCCGGGCGTGCGGCAGATGCGGTGGCGCGGCGGTAATCCGGGCTCTCGCCGTCGGAAAAGCCGATCGTGACGGAGTGCGGCAACTCGCTCTCCTGCGCCCGCACCCGGCGCAGGGGCGCCGCCTCGCCCTCGCCGAGAACGAGGTCGCCCGCGTCCAGCGCGGCGGGCGCGCTCATACGCCCGTCGCGAATCCGCAGGGCTCCCCCAGAGACCGAAACGTCGAGGTTGAACAGCCGGGCGAGCGGCTCCAGCGCCTCGCGGGCGGAGAGCGGCCGGTCGAGCATGGCCCCGTCGAGGAATCCGGCGGCATCGATGTCGAGGGGCGCGTCGATCCCGAGATCGGCCAGGATGGCCACGATCAGCCGGTCGAGTTCCAGCCCCTCGATCCGGCCGGTGATCCAGTGGCCGAGCGCCCAGTTGGCGGCGTCCGACCACAGGCTCGTGAAATCGGGGAAGGCCGGAAACGGCCGGGCATCCCAGGCCCAGACGTAGAGGTCCTCGGCCGGGATCATCCGGCCGCCATAGAGGGGCGAGACTGGATTATGAGCCGGCTCAAAACCCTCGGATTCAGGATCGAAGCGCGAGAGGATCGCGTCGAGGCCGCGGGCCTGAATCAAGTCATCGCGGCTGCCGCGGGAGAAGGGCGGCGCCGCATTCTCGGACGATTTCGGATCGGGGAAGACGTTGGGCCCGTTGGTGCCGCGATCGACCGCCGGGATGCCGATCTCGGTCAGCCAGATCGGCTTGGCGCCCGGCATCCAGGCGGTCGGCCCGGTCTCGACGCCGCCGGCCCGCTCGACATGCGGGTTCGACCACCATGCGACGAGATCCTTCGCCCGGTAGACCCACGGCTTCCCGTGGGCACCGTCGGTGATCGGCGTGCGCCGTTGCGCGGCGCGATCCTCGGGGCTGGCATAATACCAGTCGAACGCCTCGCCCGCCCCGAGGCGGTTCTTGAGATAGGCGCGGTCGTAGAGGCTCGCAGCCTCCGCCGCGTCGCGATGATCGGGCCCGTCGCGCCAGTCCGAAATCGGCGGGTAGTAGTCGATGCCGACGGCATCGATGGCCGGATCGGCAAAGAGCGGATCGAGGGGGAAGCGTACGGAGGCGCCGCCATCGCGGACATGGGCGCCGTACTCGGTCCAGTCGGCGGCGTAGACGAGGCTGACATCGCGGCCGAGGCGGGCGCGGACCTCCGTGGCGAGGCGCCGGAGTTGCACGACCGCCGGATAGGCGCCGTCCGGTCCGCGCACCCGTGTCAGGCCGACGAACTCGCTGCCAAGGATGAAACCGGCGAGCGGCGCTCCCGCCGCCGCCCAGCCGGCGGCGAGGCCGGCATAGTGCAGGATCATCGCCCGATAGCCCTGCCGCCCCTCGAAGAAGGCCGCGACCTGATCGGTCGCGCCGCCGGTCGCGTCCGGGCTTCCAGTCCGGTCGGGAGCGGGATCGCAGGTGATGCGCCCGCGCCAAGGGTAAGCCGGCTGCGTTTCGCCGGGTCGGTACGGGTCCGGCAGAGCGTTGCCGGCCGGCACGTCCATCATCACGAAGGGATAGAGCACCACCGCGAGGCCGCGGCGGGTCAGCTCCGCCACCAGCCGCCCGAGCCCGGCATCGGACGGCGTACCGCCATAGGCGGCCGTCTCACCGGAGCGGGAGACGGCCTGCGCCGTGGCGCGGTTCAGCCCGGCAACGGACCAGGGCTCGCCTGTCGTCGCCTTCGCCGTCGCGTCGACCCGCGGCGTCACGGTGCAGTGCCCGGCGCGCAAATCGTCGCCGAACCAGCTTGCCACCACCGAGACGCGCTTGAGCCGGGGGCACAAAGCCTGGAGCGCATCGAGCGAGGCGACAACGTCACTCGCGCCCTGAAACTGAAAACGGTTTGCCGGCTTTGTAGCGCCGAATCCCGCGTCCTCGCTGACGGCGAGTGGGTCGAGGCCGAACTCGGTCGAGCCCGGTATCAGGCAGACCGCGCGGATCTTGCTCGCCAGCCCGGCGACGGGGCGGATCACCTCGAAGGCGAATTGCGGGATGCGGTTGCCGAAGGCAGCGAGCGGCAGCCGCTCGAACACGATGTAAGCAAGCCCACGATAGGCCGGCGCGTTGTCCGCGCCTTCCTTGGCGACGATCAGTGGATCGGGCGCCTGCGTCGCCGAGCCGGTATGAACACGCCAATTGAGCGTCGTCAGGTCGAGTTCGCTGCCATCGGCCCAGATCCGTCGGACCAGGGCGATCTCGCCCGCGCAGAGCCCGACGGCGAGATCGATGAAGGTGCTGTAGGCGGTGCGCACGGTTTTCTGGCCGCCCGCCCCGCCCTTCGAGCCGGAGGAGGACCGCTCGACGGTGGTGTTGGCGACCTCGAGTGGGCGCGTCGCCCAGATCAGGGTGCCGCCGATGCGGGCCCGGCCATAGACGCGGGGCACCGGCGCCCCCTCCGTGGAGGCGAGGCCGCCGAGTTCGGTCAGCCGCGGGCCGTCCACGAAGCGCGGGCTCGTGCCGGCGCCGAACAGCGCGCCGTCGAGCGCCGCGCCCCCAGCCGCGCCGGCCACGCGCCCGATCATCCCGCCGATGGGACCGCCGAGCGCAGTGCCGACCGCCTCGCCGGCGGTTTGCAGGACGAGCGTCGTCATGGCGGTGACTCCGGCGGGCGCAAGGATCAGGGGATCGGTTCAGGGCGGATTGGGAAAGCGGAAGGCGTGAGCAAGGTGGCGCCGCCACCACGGCGTGAGCGCAACCTCGGTCACCGCCGCGCCATCATGGGCGTGGATCATCGCCGCCGGCCCGGTCGCGATGGCGCAGTGCTTTGCCGGCAAATGTGCGCGAAACGCGAAGAGCAGGACGTCACCGGCCCAGGGCTGATAAGCGTTCAGCGGTCCCGCGATCGGCACGAGGTGCCGCCGCGCGGCGGCGACCAGTGGGTCGGCGCCGTCGAGGGCCGATTCGGCCCACGTGGCGGCGTAGGGCGGCGCCTCCTCCGGCTCGGGGCCGAGCACCGCCCGCCAGACCCCGCGCAGCAGGCCGAGGCAGTCGCAGCCGACGCCCTTCAGCGAGGCTTGGTGCCGATAGGGCGTTCCGGCCCAGGCACGGGCCTCCGCCACGATCCGTCCGGCGAGCGTTGAGACGTCGTCATCCATGATGGCCTCATCGGAACAGGCTGGAGCCGTCGAGGCGCGCGCCGGCCTCAGGCCCGGAGCGCAGCACGAAGTCGTTGCCGGGCATGTGCGGAAAACCCTGGAAGTTGAGGGTATTGGCGAACTTGTCCCGGCAGGTGGCAAGGCTCTTGTCGCAGCCGGCGACGAGCGTGAAGGCATCGCCGGTCACAACCGGGCGCGGCGGCGGGTCCCACAGGTCGAGGATTGTCGCCGCGCCGTCGCGGCGATGGCCGCGCAGATCCGCGGCGTGTCCGGCATTGGCGCCGGTGTCCCAGGTCAGGCGACCGCCGGAGAACCAGTCGGTCGTGAAGGTCCCCGCCAGCACGACGCTGAGCCGGCCAGGCTCGGACGCGGCCCGTACGGTGCCGGTCGCGCGGAAGCGCAGGCCGGCGAGATCGACGCGGCAGCGGGCATCGCCGAGTTCCGCGTCGCAGACGGCGCGGAAGCTGCGCCCGACCGTGACGTCGAGGCGGTGCATCAGCCCGCGCAGTTCGGCGACGAAGGCGCCCCCCTCCCGGCGGATCTCGCCCAGGGTCGCCACGTCGAGGAGCAGACGCGCCTCGGGCTCGGCCCAATCGACCAGCCAGGTCTCGACCCCGGCCCCGTCATAAAGCCCCGCCGCGACGTCGATCTCCGTGATCCCGGCCGAGGTCAGCGCCCCGGCCACCTCGCCGCCGCCGACGGCGAAGCCGAGTTCCGCGCTGGCCTCGGCCGCCTCCAGCCCGCTGCGGGCGGTGTAGGTCACGCCACTGAGGACGAGATCGCGGTCGTGGTCGGTGAAGCCGAGGACGAGTCCGTCGCGGCGGCGCAGGGCCCAGCAGCGGCAGAGCGTGGTCGCGCCGCCCTCCAGGCGCGCGGCGAGACGGGGCGGGACGGCGCGCATGGGCTTCCCCTCAGGGCAGGATCTCGATCAGCGGGATGCGCGGCACCTCGCCGGCGGTGAAGGCGGCGAGATCGATGGTCAGTTCGTCGGTGTCGAAGCGGACCGGCACGTCGAACTCGAAACCGGCGGTGACGGCGGAGCCTGGTGGCGGCACCGCGTCGGCGGCGAAGGTGACCCGGCCCGTGGCCGGATCGACAGCGAGTTTCGGGGCTGCCACCTCGGCACCGTTCACTGCCACGCGCACAGTGCCGGCCACCGGCTTGGCGATCGCCCGGCGGTAAGCCTCCGGGCCGCTCCCGTAGGTCTTGGCAAGGGCAAAGATGCGGGTCGCGCCGTCGCCGGTGCCGATCCGTTGGTCGGTCGGCTCAGGCGGCCGCGAGGGCGAGCCGGAGCGGTGATCGATCCGGTCGCGGTAGCGGAACCCGTAGAGCCGCCCGCGCCGCTCCTCGAAGAAGCCGAGCACCGCATGCAGGGCGTCGAGGGTGCGGATGCCGAGCCCGGCATCGTAGCGGCGGCGCGAATCGGCCCAGCGGCTGTTGCGGTGCTCGCGGCCCGAGGCCAGCGTGACGATCTCGGTGCGCCGCACCGGGCCGCCGCTGCCGCGCAGGGCGACATCGAGGGGGAAGCGAACCTCGTGAAAGTCGGATGCCATGACGGTGCTCCTCAGAGCGCCCGTCGGCCGCGTGCCACGGCGCGGGCGAGGCGGGCAGCGATCTGGGCCTCGGAGCGGCGGAAACCCTCGATGTCCGGGGTGGCGATGTTGACGGTGACCGCGACCGGACGCTCGGCCCCGCCCGCCGCGACGCCGAGACGGCCGTCGCTGCCGCGCCTGAGCGGCAGGATCGCCTCCGGCCCGGCCTCGCCCATCAGGCCGGCGTCGCCCCCAGGGCCGGCAAGCGGAAAGTAGGTCGGCGCCGCCACGACCCCGCCGGCCGCGAAGGGGCGCACCCGTCCCCCGCCGAGAACGCCGCCAAGGCGGAACGGCACGATCCGCCCATCGGAGACGATGCCGCCTTTAGCGAGCGCCGTCGTGCCGCCGCCCTCGGAGGCCGCGCTCAGCAGGCTGTTGACGAGACTCGTCACGCCCGCCTTGACCGGAGCCAGCGCCGCCTTGACCGCGACGTTGGAGAGCTTGCTCGCCAGCGAGGCCAGCACGCCGTCGAGCTGGCGCCCGGCATTGAGATTGCGGTCGAAGGCCGAGGACAGGCTGTGGCCGAAGCGCTGGGCCAGCCGGTCGAGGGTTTCGAGCTGCCTGGCGCGCTCGGCCGTGCTGCGGTCGCTGTCGGTCATCACGGGGTCCCCGGATCGGGATGGGCGGCGAGCAGCCGTTCGAGATCGGCCCGGCTCGGCGCGTCGGGCGCCGGGCGGGTCAGGCCCGCGGCGGCGGCGAGTTCGCGGGGCGTGGCGGCCCAGAGGTCGCGCGGGCGCCAGCGCAGGGTCGCGAGACCGAGCGTCAGCACCGCATCCCACGGGAAGGCGGAGACGGGCGTCTCCGGCGCGTGGCTTCCCGTGAGGGTCAAGGGTCCGCGGGCGGCGCCTCGCCGAAGGCCGCGACCAGCGCCTCCCCGAGCGCCGAAGTCGCGGCCGCCAATCCGCCGGAGAGCGGAAGGCGGGCGACCGCCTCGTCGTCGAGGGCGTGGCCGCCGCCGCGGAGTGCCGCGCCGAGCAGCGCGATCACGTCACGGGCGGCGAGCCGCCCGCCGGCAAAGCGCTCGGCCAGGCCGGCGAGGTCGCCCGCGCCGAGCGCGTCTTCCAGTTCGGCGAGCGCGCCGAGGGTGAGGCAGAGCGTGTAGCGCTCCTGGCCTAAGGTCAGCGGCACCTCGCCGCGTCGGCGGTTGGCCATCGTCATGCCCCCGCGAAGCTCAAGGGGCCGGCGGAATCGAGGGCCATGTCGAAGGTCACCTCGCCCGCATGCTCGCCGCGATACTCAAGGCTCGTGATCTGGAACAGGCCCTCGATCGTGCCGAAATCCGGCACCACGATCTGAAACAGGCCGATCACGCCATCGAAGAACATCTGGCGCAGGCGTGCATCGGAGGCCTCGTCGCGGAACACACCTGATCCGGCGACCGCGGCGCGGCGCACACCTGCGCCGGCCAGCAGCTCGCGCCAGCGCCCGGCGGAATCGGCGTTGGTGACGTCCACCGTCTCAGCGTTGAAGGCGAGTTGCCGGGCGCGCAGGCCCGCCACCGCGGCGAAGCCGCCGGCCCCGTTACTGACTCGGACCAGGAGGTCCCTGCCTCTCTGTGCGCTCATGCTGGCGGCTCCGGCGTTCTTGAGGAAAATCAGGCGGCAACCTCGGTCACCGCCTCGAAGGAAAGGGTCGCGCGCGCCTCACCGGTGCGCTCGTCGCGCAGGCCGGCGAGGCTCTTGAGGCGCAGGAAGACGAGGGCGTGGCCGCTCACGGCGAGGTCGGCCTCGTCGAGGCGCGTGGCGATCCGCTCGGCCACCTCCAGGGCCGAGCGGGCCGAGCCCGGCTTGGCGAAGACCGTCAGCGCGAAGGCGTGGCGGTGTCGCTGCGCACCGTCGACCGAGTCGTCGCTGATCTCGCTCGGCCCGAATAGCGCGTAGACTGGGGCGGCGCCCCGTGGCGGCTCATCGTAGAGCCGCAGGCGGCCGCCCATCAGCGCGGCGAGCGGCGTATCCGCGGCAAGATGAGCGAGAAGGCCGGCGCGCAGGGCCAGCAGCGGGCTCGAACGCGTCACGGGGAAGCCTCCTCGATCACCTCTTCCACCTCGCAGACGAGGTCGCGGCGGCACCCATCGGGGTCGTCGGCCGCGCGGATCGCGAAGCGGCGAGGTCCGTCGGCGAGGCGCATGGCCGGAGTGACGCCCGGGCGGTAGCGCAGGCCGACGCGGTAGAGGCCGGGCCGGTCGGTGCGCCCGCCACGGGGCGCAGCGGCGCTGCCGAGCGACTCCAGCGAGCCCCACAGCACGGGTCCGGCGACGTAGCAGCGGAGCCGGCCGCCGAACCCGTCGGGCTCGTCCACCGGGACTTCGAGCACGAGGCGACGCCGCCGCGCGCCGAGCGGCCTATGTGTCATGGTCGGTCTCCGTGGGTATGGTCAGAGCCGCATCCGGCGGCAGGCGGCGGCGAGCCCCGCCGCCTCGGGCGGCAGCGGCCGGGGCGCGCCGTCGCCGCCCGCATCGCCCCGGTTCTCGAACCAGTCGGCGATCGTCAGGCGCAGGGCCTGAACCAGCGGCTCGGGGACCGGCGGCCCCTCGCCGCCGCAGCCGGCGCTGACCTCGACCAGCGCGGAGGCCGTGCCGAGCGGCGGCAGGTCCGGCCCGAACACGAGGCAGGGCACCTCCCATGGATCGGGGCCGAGCCGGATCGGGCCCGGCTCGATCTCGGTGAGGGCGCCGCGCCCATCGACCAGCCTGGCGCGAAGCACGGCGACGAGCGGGCTCAGCGGCAGCGGCATCAGCCCGAGCCGGGGCCAGGCTGCCAGCACCACCCGGAAACGGGCGGGCCGCAGCAGCCGGCGTGCGGTCGCCTCGACCGCCGCTCGCGCCGCCGGGATCAGGCGGGCGATCAAGGCGTCCTCGGCGGTCTCGTCGGAGGACAGCCGGAGATAGCCGCGCATCTCGGCCACGGTCACCGGCTCGACGCCGGTCTCCTCGACGCGTATCGGGGTCATGCCAGCCTCTCCTGAAAGCGTTTTTCGCAATCGATGCGCGCGCCGAACCCATCTCTTCCGCGCAGTGCAGGCCTTGGCCTCGGGGCGGCGGCGCTCACGCTCCTGCTCGCAGCCACGCCGGCGACGGCCATCGTCGGCGGCCGGGAGGGCGCCGAGGTGCCGGGCGCGGCCTCGGCGGTGATGGTGCTGACCTCCGGCGGCGGGGTCTGTTCAGGGATCGTCGTCGCGCCGGACGCGGTGCTCACCGCCGGCCATTGCGTGGCGGGCGTGGGCGAGAACCGCGTCCATTACCGCGACGAAACAGGCCGTCCGGTGCTGGCCGAGACCGCCGCCCGCAGCTTGCATCCGGCCTATGACGGCGATGCCATCCGCGGGCGCACCCGCTCTATCGATCTCGCGCTGCTGCGCACCCGCGAGCCCCTGCCCGCCCGGTTCGTGCCGGCCGCGTTGAGCGCCGCGATGCCGCGGGCCGGCCAGAGCCTGACGCTCGCCGGCTACGGCGCCGCGCGGGGCGGCGACCGTCGCTCGATCGGCCGCTATCGCGGCGCAGCGCTCAATGTCGTCGAGCCCTACGGCCCGAGCCGCATCCTAGTTTGGCTGAAGGCATCGGGAGCGGGCGGCTGCCAGGGCGATTCCGGCGGGCCGATCCTCGACGGCGCGGCAGTGGTCGCGGTCGCCGCATGGGTGAAGGATGTCTGCGGCGGCCTGACTCAAGGCATCCTGCTCGGGCCGCAGCGCGACTGGATCGACCGGACGCTGTCCGGCTGGGGCGCCTCCGCGCGCTGGTGA

Protein sequences of DBSCAN-SWA_2

>NC_010172|1589389:1600923|1598010_1598337_-|WP_012253048.1|DBSCAN-SWA
MANRRRGEVPLTLGQERYTLCLTLGALAELEDALGAGDLAGLAERFAGGRLAARDVIALLGAALRGGGHALDDEAVARLPLSGGLAAATSALGEALVAAFGEAPPADP
>NC_010172|1589389:1600923|1595128_1595593_-|WP_012253043.1|DBSCAN-SWA
MDDDVSTLAGRIVAEARAWAGTPYRHQASLKGVGCDCLGLLRGVWRAVLGPEPEEAPPYAATWAESALDGADPLVAAARRHLVPIAGPLNAYQPWAGDVLLFAFRAHLPAKHCAIATGPAAMIHAHDGAAVTEVALTPWWRRHLAHAFRFPNPP
>NC_010172|1589389:1600923|1597783_1598014_-|WP_012253047.1|tail|DBSCAN-SWA
MTLTGSHAPETPVSAFPWDAVLTLGLATLRWRPRDLWAATPRELAAAAGLTRPAPDAPSRADLERLLAAHPDPGTP
>NC_010172|1589389:1600923|1596504_1597146_-|WP_012253045.1|DBSCAN-SWA
MASDFHEVRFPLDVALRGSGGPVRRTEIVTLASGREHRNSRWADSRRRYDAGLGIRTLDALHAVLGFFEERRGRLYGFRYRDRIDHRSGSPSRPPEPTDQRIGTGDGATRIFALAKTYGSGPEAYRRAIAKPVAGTVRVAVNGAEVAAPKLAVDPATGRVTFAADAVPPPGSAVTAGFEFDVPVRFDTDELTIDLAAFTAGEVPRIPLIEILP
>NC_010172|1589389:1600923|1598339_1598750_-|WP_012253049.1|tail|DBSCAN-SWA
MSAQRGRDLLVRVSNGAGGFAAVAGLRARQLAFNAETVDVTNADSAGRWRELLAGAGVRRAAVAGSGVFRDEASDARLRQMFFDGVIGLFQIVVPDFGTIEGLFQITSLEYRGEHAGEVTFDMALDSAGPLSFAGA
>NC_010172|1589389:1600923|1595600_1596494_-|WP_012253044.1|DBSCAN-SWA
MRAVPPRLAARLEGGATTLCRCWALRRRDGLVLGFTDHDRDLVLSGVTYTARSGLEAAEASAELGFAVGGGEVAGALTSAGITEIDVAAGLYDGAGVETWLVDWAEPEARLLLDVATLGEIRREGGAFVAELRGLMHRLDVTVGRSFRAVCDAELGDARCRVDLAGLRFRATGTVRAASEPGRLSVVLAGTFTTDWFSGGRLTWDTGANAGHAADLRGHRRDGAATILDLWDPPPRPVVTGDAFTLVAGCDKSLATCRDKFANTLNFQGFPHMPGNDFVLRSGPEAGARLDGSSLFR
>NC_010172|1589389:1600923|1600167_1600923_+|WP_012253053.1|protease|DBSCAN-SWA
MRAPNPSLPRSAGLGLGAAALTLLLAATPATAIVGGREGAEVPGAASAVMVLTSGGGVCSGIVVAPDAVLTAGHCVAGVGENRVHYRDETGRPVLAETAARSLHPAYDGDAIRGRTRSIDLALLRTREPLPARFVPAALSAAMPRAGQSLTLAGYGAARGGDRRSIGRYRGAALNVVEPYGPSRILVWLKASGAGGCQGDSGGPILDGAAVVAVAAWVKDVCGGLTQGILLGPQRDWIDRTLSGWGASARW
>NC_010172|1589389:1600923|1599558_1600134_-|WP_012253052.1|DBSCAN-SWA
MTPIRVEETGVEPVTVAEMRGYLRLSSDETAEDALIARLIPAARAAVEATARRLLRPARFRVVLAAWPRLGLMPLPLSPLVAVLRARLVDGRGALTEIEPGPIRLGPDPWEVPCLVFGPDLPPLGTASALVEVSAGCGGEGPPVPEPLVQALRLTIADWFENRGDAGGDGAPRPLPPEAAGLAAACRRMRL
>NC_010172|1589389:1600923|1598779_1599199_-|WP_012253050.1|DBSCAN-SWA
MTRSSPLLALRAGLLAHLAADTPLAALMGGRLRLYDEPPRGAAPVYALFGPSEISDDSVDGAQRHRHAFALTVFAKPGSARSALEVAERIATRLDEADLAVSGHALVFLRLKSLAGLRDERTGEARATLSFEAVTEVAA
>NC_010172|1589389:1600923|1591180_1595089_-|WP_012253042.1|tail|DBSCAN-SWA
MTTLVLQTAGEAVGTALGGPIGGMIGRVAGAAGGAALDGALFGAGTSPRFVDGPRLTELGGLASTEGAPVPRVYGRARIGGTLIWATRPLEVANTTVERSSSGSKGGAGGQKTVRTAYSTFIDLAVGLCAGEIALVRRIWADGSELDLTTLNWRVHTGSATQAPDPLIVAKEGADNAPAYRGLAYIVFERLPLAAFGNRIPQFAFEVIRPVAGLASKIRAVCLIPGSTEFGLDPLAVSEDAGFGATKPANRFQFQGASDVVASLDALQALCPRLKRVSVVASWFGDDLRAGHCTVTPRVDATAKATTGEPWSVAGLNRATAQAVSRSGETAAYGGTPSDAGLGRLVAELTRRGLAVVLYPFVMMDVPAGNALPDPYRPGETQPAYPWRGRITCDPAPDRTGSPDATGGATDQVAAFFEGRQGYRAMILHYAGLAAGWAAAGAPLAGFILGSEFVGLTRVRGPDGAYPAVVQLRRLATEVRARLGRDVSLVYAADWTEYGAHVRDGGASVRFPLDPLFADPAIDAVGIDYYPPISDWRDGPDHRDAAEAASLYDRAYLKNRLGAGEAFDWYYASPEDRAAQRRTPITDGAHGKPWVYRAKDLVAWWSNPHVERAGGVETGPTAWMPGAKPIWLTEIGIPAVDRGTNGPNVFPDPKSSENAAPPFSRGSRDDLIQARGLDAILSRFDPESEGFEPAHNPVSPLYGGRMIPAEDLYVWAWDARPFPAFPDFTSLWSDAANWALGHWITGRIEGLELDRLIVAILADLGIDAPLDIDAAGFLDGAMLDRPLSAREALEPLARLFNLDVSVSGGALRIRDGRMSAPAALDAGDLVLGEGEAAPLRRVRAQESELPHSVTIGFSDGESPDYRRATASAARPAGARRQEVRVDAAIVTRRDAVQALAEAVLDAALAGRDTAAFGVSPRRIDLEPGDVVALPDADSGTLHRIVRIDDAPTGRRIETRAVPLPRSDRAPTTPAPRTGRRPPAVPGPVFALPLVLPVERGDPVPLAYLAVSADPWPGAVAVWRSAGEGAPFALHRTLDHPACLGRTLTPLPPGPLWRFDRNARLDVTLRHAEGLSAIGEAAALAGGNLFALVGADGTVELLSAAKAELIGAGTWRLSSLLRGLAGSEAAAGRSAGTGSLIVRLDDGAVRPLVDRLDEVGRRFLYRVGPAERDPADPTFVGFAATADLTAFTPLSPVHLRARREAEGIRFHWIRRARRDADAWEPADLPPGAGAEAYRLDILAEDGRILRSLSTATTSALYARADEIADFGTPRTEIDAALVPIGSLAGPGPARRTRVSLRTV
>NC_010172|1589389:1600923|1589389_1589953_-|WP_012253040.1|DBSCAN-SWA
MDLSAVGRAVLIAREGRRLEAYRDSVGVWTIGIGHTAAAGPPVPRAGLRIEAGEADAIFTRDVAAFVRTVAEAIPEPLPQHAFDALVSLCFNIGPAAFRRSTVLRRLRAGDRAGAGEAILMWNRPAAIIPRRQGEFDQFHTPYETALPRARRSDAQPVAAPVERPSSPSSARPGWRARLWDWLRAGR
>NC_010172|1589389:1600923|1590073_1591156_-|WP_012253041.1|DBSCAN-SWA
MADTTPSLALPLIAGGQAQKHVTHNEALALLDALVQLAVLDKDRAAPPASPAEGDRYLIVSGNPSGAWTGWAGRIARFQDGAWLSLKPLVGWTAYVADEAELYTFTGSAWMPFRATITALDNLTRLGLGTGADVDNPFAAKLNKALWTALTVAEGGSGDLRYTLNKQATGNTLSLLMQTGFSARAEIGLTGDDDLRVKVSPNGGDWFEALRIGRETGRVAFPGRITVADVPVLTAQVIAGNSGPGSIPAGATRYFTNALVGGHPSVVYAAAGRRGRFRDLRVVTQGAPGAGQSWTVTLQNLFTGTALTCTIAGAGSNQAADLVNSAVFEAADRWCLKIVPSSGAQPTTNILFSLLFEGLD
>NC_010172|1589389:1600923|1599195_1599537_-|WP_012253051.1|head,tail|DBSCAN-SWA
MTHRPLGARRRRLVLEVPVDEPDGFGGRLRCYVAGPVLWGSLESLGSAAAPRGGRTDRPGLYRVGLRYRPGVTPAMRLADGPRRFAIRAADDPDGCRRDLVCEVEEVIEEASP
>NC_010172|1589389:1600923|1597157_1597784_-|WP_012253046.1|tail|DBSCAN-SWA
MTDSDRSTAERARQLETLDRLAQRFGHSLSSAFDRNLNAGRQLDGVLASLASKLSNVAVKAALAPVKAGVTSLVNSLLSAASEGGGTTALAKGGIVSDGRIVPFRLGGVLGGGRVRPFAAGGVVAAPTYFPLAGPGGDAGLMGEAGPEAILPLRRGSDGRLGVAAGGAERPVAVTVNIATPDIEGFRRSEAQIAARLARAVARGRRAL

Homologous phage analysis in the prophage region

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_012253040.1|1589389_1589953_-	lysozyme	W6E9Q2	Rhizobium_phage	1.8e-25	41.4
WP_012253041.1|1590073_1591156_-	DUF2793 domain-containing protein	A0A0K1Y6G9	Rhodobacter_phage	3.4e-36	40.0
WP_012253042.1|1591180_1595089_-|tail	glycoside hydrolase/phage tail family protein	A0A0B5A7K5	Paracoccus_phage	1.1e-196	36.2
WP_012253043.1|1595128_1595593_-	peptidase P60	A0A1V0DYB6	Dinoroseobacter_phage	4.2e-28	46.4
WP_012253044.1|1595600_1596494_-	DUF2163 domain-containing protein	A0A0K0PVK1	Roseobacter_phage	1.4e-56	41.2
WP_012253045.1|1596504_1597146_-	DUF2460 domain-containing protein	A0A0B5A2K3	Paracoccus_phage	1.6e-46	50.5
WP_012253046.1|1597157_1597784_-|tail	phage tail tape measure protein	G9JXH3	Shigella_phage	2.5e-07	69.0
WP_012253047.1|1597783_1598014_-|tail	phage tail assembly chaperone	NA	NA	NA	NA
WP_012253048.1|1598010_1598337_-	gene transfer agent family protein	NA	NA	NA	NA
WP_012253049.1|1598339_1598750_-|tail	phage major tail protein, TP901-1 family	NA	NA	NA	NA
WP_012253050.1|1598779_1599199_-	DUF3168 domain-containing protein	NA	NA	NA	NA
WP_012253051.1|1599195_1599537_-|head,tail	head-tail adaptor protein	NA	NA	NA	NA
WP_012253052.1|1599558_1600134_-	phiE125 gp8 family protein	NA	NA	NA	NA
WP_012253053.1|1600167_1600923_+|protease	trypsin-like serine protease	NA	NA	NA	NA