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Overview of predicted results

Overview of the results

Contig_ID Contig_def CRISPR array number Contig Signature genes Self targeting spacer number Target MGE spacer number Prophage number Anti-CRISPR protein number
NC_012563 Clostridium botulinum A2 str. Kyoto, complete genome 5 crisprs DEDDh,csa3,DinG,WYL,cas3 1 9 7 3

Results visualization

1. NC_012563
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CRISPR_ID CRISPR_location CRISPR_type Repeat_type Spacer_info Cas_protein_info CRISPR-Cas_info
NC_012563_1 1179524-1179875 Orphan III-B
5 spacers

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CRISPR_ID CRISPR_location CRISPR_type Repeat_type Spacer_info Cas_protein_info CRISPR-Cas_info
NC_012563_2 2455808-2456100 Orphan III-B
4 spacers

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CRISPR_ID CRISPR_location CRISPR_type Repeat_type Spacer_info Cas_protein_info CRISPR-Cas_info
NC_012563_3 2459891-2460115 Orphan III-B
3 spacers

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CRISPR_ID CRISPR_location CRISPR_type Repeat_type Spacer_info Cas_protein_info CRISPR-Cas_info
NC_012563_4 2563318-2563804 Orphan III-B
7 spacers

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CRISPR_ID CRISPR_location CRISPR_type Repeat_type Spacer_info Cas_protein_info CRISPR-Cas_info
NC_012563_5 2655984-2656343 Orphan III-B
5 spacers

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CRISPR_ID Spacer_Info Spacer_region Spacer_length Hit_ID Protospacer_location Mismatch Identity
NC_012563_5 5.2|2656081|36|NC_012563|CRISPRCasFinder 2656081-2656116 36 NC_012563.1 1708437-1708472 1 0.972

1. spacer 5.2|2656081|36|NC_012563|CRISPRCasFinder matches to position: 1708437-1708472, mismatch: 1, identity: 0.972

aattatggctaatggatttgatactacacaattaga	CRISPR spacer
aattatggctaatggatttaatactacacaattaga	Protospacer
*******************.****************

CRISPR_ID Spacer_Info Spacer_region Spacer_length Hit_phage_ID Hit_phage_def Protospacer_location Mismatch Identity
NC_012563_3 3.3|2460051|35|NC_012563|PILER-CR,CRISPRCasFinder 2460051-2460085 35 NZ_CP013700 Clostridium botulinum strain AM1195 plasmid pRSJ11_1, complete sequence 121453-121487 1 0.971
NC_012563_3 3.3|2460051|35|NC_012563|PILER-CR,CRISPRCasFinder 2460051-2460085 35 NZ_CP006909 Clostridium botulinum CDC_1436 plasmid pCBG, complete sequence 164047-164081 1 0.971
NC_012563_3 3.3|2460051|35|NC_012563|PILER-CR,CRISPRCasFinder 2460051-2460085 35 NZ_CP013684 Clostridium botulinum strain AM282 plasmid pRSJ10_1, complete sequence 52651-52685 1 0.971
NC_012563_4 4.5|2563609|35|NC_012563|CRISPRCasFinder,CRT,PILER-CR 2563609-2563643 35 NZ_CP031100 Clostridium botulinum strain CFSAN034202 plasmid p1_CFSAN034202, complete sequence 1221-1255 1 0.971
NC_012563_4 4.5|2563609|35|NC_012563|CRISPRCasFinder,CRT,PILER-CR 2563609-2563643 35 NZ_CP014173 Clostridium botulinum strain B609 plasmid pRSJ22_2, complete sequence 21192-21226 1 0.971
NC_012563_4 4.5|2563609|35|NC_012563|CRISPRCasFinder,CRT,PILER-CR 2563609-2563643 35 NZ_CP014218 Clostridium botulinum strain B515 plasmid pRSJ21_3, complete sequence 30043-30077 1 0.971
NC_012563_1 1.1|1179552|39|NC_012563|CRISPRCasFinder,CRT 1179552-1179590 39 NZ_CP014218 Clostridium botulinum strain B515 plasmid pRSJ21_3, complete sequence 44654-44692 2 0.949
NC_012563_4 4.5|2563609|35|NC_012563|CRISPRCasFinder,CRT,PILER-CR 2563609-2563643 35 NZ_CP014147 Clostridium botulinum strain CDC_67190 plasmid pNPD12_2, complete sequence 35933-35967 2 0.943
NC_012563_1 1.1|1179552|39|NC_012563|CRISPRCasFinder,CRT 1179552-1179590 39 NZ_CP014173 Clostridium botulinum strain B609 plasmid pRSJ22_2, complete sequence 6458-6496 3 0.923
NC_012563_1 1.1|1179552|39|NC_012563|CRISPRCasFinder,CRT 1179552-1179590 39 NZ_CP014147 Clostridium botulinum strain CDC_67190 plasmid pNPD12_2, complete sequence 48962-49000 3 0.923
NC_012563_1 1.1|1179552|39|NC_012563|CRISPRCasFinder,CRT 1179552-1179590 39 NZ_CP031100 Clostridium botulinum strain CFSAN034202 plasmid p1_CFSAN034202, complete sequence 45867-45905 3 0.923
NC_012563_2 2.1|2455838|35|NC_012563|CRISPRCasFinder,CRT 2455838-2455872 35 NZ_CP013709 Clostridium botulinum strain F634 plasmid pRSJ2_2, complete sequence 138413-138447 3 0.914
NC_012563_2 2.1|2455838|35|NC_012563|CRISPRCasFinder,CRT 2455838-2455872 35 NZ_CP013682 Clostridium botulinum strain 1169 plasmid pRSJ8_1, complete sequence 141490-141524 3 0.914
NC_012563_2 2.1|2455838|35|NC_012563|CRISPRCasFinder,CRT 2455838-2455872 35 NZ_CP013295 Clostridium botulinum strain CDC_54064 plasmid pNPD1_1, complete sequence 152206-152240 3 0.914
NC_012563_2 2.1|2455838|35|NC_012563|CRISPRCasFinder,CRT 2455838-2455872 35 NZ_CP013844 Clostridium botulinum strain A634 plasmid pRSJ19_2, complete sequence 183850-183884 3 0.914
NC_012563_4 4.4|2563543|36|NC_012563|CRISPRCasFinder,CRT 2563543-2563578 36 KM983327 Clostridium phage phiCT453A, complete genome 27142-27177 5 0.861
NC_012563_4 4.5|2563609|35|NC_012563|CRISPRCasFinder,CRT,PILER-CR 2563609-2563643 35 MH319752 Marine virus AG-345-F15 Ga0172271_14 genomic sequence 2573-2607 6 0.829
NC_012563_3 3.6|2460042|44|NC_012563|CRT 2460042-2460085 44 NZ_CP006909 Clostridium botulinum CDC_1436 plasmid pCBG, complete sequence 164038-164081 8 0.818
NC_012563_3 3.6|2460042|44|NC_012563|CRT 2460042-2460085 44 NZ_CP013684 Clostridium botulinum strain AM282 plasmid pRSJ10_1, complete sequence 52642-52685 8 0.818
NC_012563_3 3.6|2460042|44|NC_012563|CRT 2460042-2460085 44 NZ_CP013700 Clostridium botulinum strain AM1195 plasmid pRSJ11_1, complete sequence 121453-121496 8 0.818
NC_012563_4 4.1|2563348|36|NC_012563|CRISPRCasFinder,CRT 2563348-2563383 36 NC_010180 Bacillus mycoides KBAB4 plasmid pBWB401, complete sequence 393605-393640 8 0.778
NC_012563_2 2.4|2456036|35|NC_012563|CRISPRCasFinder,CRT,PILER-CR 2456036-2456070 35 MN693317 Marine virus AFVG_25M47, complete genome 27582-27616 9 0.743
NC_012563_4 4.7|2563740|35|NC_012563|CRISPRCasFinder,CRT,PILER-CR 2563740-2563774 35 NC_041878 Pectobacterium phage CBB, complete genome 110155-110189 9 0.743
NC_012563_2 2.4|2456036|35|NC_012563|CRISPRCasFinder,CRT,PILER-CR 2456036-2456070 35 MH586730 Salmonella phage Solent, complete genome 12186-12220 10 0.714
NC_012563_2 2.4|2456036|35|NC_012563|CRISPRCasFinder,CRT,PILER-CR 2456036-2456070 35 NC_047786 Salmonella phage vB_SenS_Sasha, complete genome 9067-9101 10 0.714
NC_012563_2 2.4|2456036|35|NC_012563|CRISPRCasFinder,CRT,PILER-CR 2456036-2456070 35 KY002061 Salmonella phage vB_SenS_Sergei, complete genome 11999-12033 10 0.714

1. spacer 3.3|2460051|35|NC_012563|PILER-CR,CRISPRCasFinder matches to NZ_CP013700 (Clostridium botulinum strain AM1195 plasmid pRSJ11_1, complete sequence) position: , mismatch: 1, identity: 0.971

agaaacccttgtggatgtggttctaatgtttttca	CRISPR spacer
agaaacccttgtggatgtggttctaatatttttca	Protospacer
***************************.*******

2. spacer 3.3|2460051|35|NC_012563|PILER-CR,CRISPRCasFinder matches to NZ_CP006909 (Clostridium botulinum CDC_1436 plasmid pCBG, complete sequence) position: , mismatch: 1, identity: 0.971

agaaacccttgtggatgtggttctaatgtttttca	CRISPR spacer
agaaacccttgtggatgtggttctaatatttttca	Protospacer
***************************.*******

3. spacer 3.3|2460051|35|NC_012563|PILER-CR,CRISPRCasFinder matches to NZ_CP013684 (Clostridium botulinum strain AM282 plasmid pRSJ10_1, complete sequence) position: , mismatch: 1, identity: 0.971

agaaacccttgtggatgtggttctaatgtttttca	CRISPR spacer
agaaacccttgtggatgtggttctaatatttttca	Protospacer
***************************.*******

4. spacer 4.5|2563609|35|NC_012563|CRISPRCasFinder,CRT,PILER-CR matches to NZ_CP031100 (Clostridium botulinum strain CFSAN034202 plasmid p1_CFSAN034202, complete sequence) position: , mismatch: 1, identity: 0.971

tcacaaaagagtaatactaatgttcaacaattagg	CRISPR spacer
tcacaaaagagtaatactaatgttcaacaactagg	Protospacer
******************************.****

5. spacer 4.5|2563609|35|NC_012563|CRISPRCasFinder,CRT,PILER-CR matches to NZ_CP014173 (Clostridium botulinum strain B609 plasmid pRSJ22_2, complete sequence) position: , mismatch: 1, identity: 0.971

tcacaaaagagtaatactaatgttcaacaattagg	CRISPR spacer
tcacaaaagagtaatactaatgttcaacaactagg	Protospacer
******************************.****

6. spacer 4.5|2563609|35|NC_012563|CRISPRCasFinder,CRT,PILER-CR matches to NZ_CP014218 (Clostridium botulinum strain B515 plasmid pRSJ21_3, complete sequence) position: , mismatch: 1, identity: 0.971

tcacaaaagagtaatactaatgttcaacaattagg	CRISPR spacer
tcacaaaagagtaatactaatgttcaacaactagg	Protospacer
******************************.****

7. spacer 1.1|1179552|39|NC_012563|CRISPRCasFinder,CRT matches to NZ_CP014218 (Clostridium botulinum strain B515 plasmid pRSJ21_3, complete sequence) position: , mismatch: 2, identity: 0.949

caagggtatttaaaagcaatatagaagtaatattataga	CRISPR spacer
caagggtatttaaaagcaatatagaagtaatattataat	Protospacer
*************************************. 

8. spacer 4.5|2563609|35|NC_012563|CRISPRCasFinder,CRT,PILER-CR matches to NZ_CP014147 (Clostridium botulinum strain CDC_67190 plasmid pNPD12_2, complete sequence) position: , mismatch: 2, identity: 0.943

tcacaaaagagtaatactaatgttcaacaattagg	CRISPR spacer
tctcaaaagagtaacactaatgttcaacaattagg	Protospacer
** ***********.********************

9. spacer 1.1|1179552|39|NC_012563|CRISPRCasFinder,CRT matches to NZ_CP014173 (Clostridium botulinum strain B609 plasmid pRSJ22_2, complete sequence) position: , mismatch: 3, identity: 0.923

caagggtatttaaaagcaatatagaagtaatattataga	CRISPR spacer
caagggtatttaaaagcaatatagaagtaatattattat	Protospacer
************************************ . 

10. spacer 1.1|1179552|39|NC_012563|CRISPRCasFinder,CRT matches to NZ_CP014147 (Clostridium botulinum strain CDC_67190 plasmid pNPD12_2, complete sequence) position: , mismatch: 3, identity: 0.923

caagggtatttaaaagcaatatagaagtaatattataga	CRISPR spacer
caagggtatttaaaaacaatatagaagtaatattataat	Protospacer
***************.*********************. 

11. spacer 1.1|1179552|39|NC_012563|CRISPRCasFinder,CRT matches to NZ_CP031100 (Clostridium botulinum strain CFSAN034202 plasmid p1_CFSAN034202, complete sequence) position: , mismatch: 3, identity: 0.923

caagggtatttaaaagcaatatagaagtaatattataga	CRISPR spacer
caagggtatttaaaaacaatatagaagtaatattataat	Protospacer
***************.*********************. 

12. spacer 2.1|2455838|35|NC_012563|CRISPRCasFinder,CRT matches to NZ_CP013709 (Clostridium botulinum strain F634 plasmid pRSJ2_2, complete sequence) position: , mismatch: 3, identity: 0.914

tacttgtgattttaaaataaagaacattaggtagt	CRISPR spacer
tacttgtgattttaaaataaaaaatattaggtaga	Protospacer
*********************.**.********* 

13. spacer 2.1|2455838|35|NC_012563|CRISPRCasFinder,CRT matches to NZ_CP013682 (Clostridium botulinum strain 1169 plasmid pRSJ8_1, complete sequence) position: , mismatch: 3, identity: 0.914

tacttgtgattttaaaataaagaacattaggtagt	CRISPR spacer
tacttgtgattttaaaataaaaaatattaggtaga	Protospacer
*********************.**.********* 

14. spacer 2.1|2455838|35|NC_012563|CRISPRCasFinder,CRT matches to NZ_CP013295 (Clostridium botulinum strain CDC_54064 plasmid pNPD1_1, complete sequence) position: , mismatch: 3, identity: 0.914

tacttgtgattttaaaataaagaacattaggtagt	CRISPR spacer
tacttgtgattttaaaataaaaaatattaggtaga	Protospacer
*********************.**.********* 

15. spacer 2.1|2455838|35|NC_012563|CRISPRCasFinder,CRT matches to NZ_CP013844 (Clostridium botulinum strain A634 plasmid pRSJ19_2, complete sequence) position: , mismatch: 3, identity: 0.914

tacttgtgattttaaaataaagaacattaggtagt	CRISPR spacer
tacttgtgattttaaaataaaaaatattaggtaga	Protospacer
*********************.**.********* 

16. spacer 4.4|2563543|36|NC_012563|CRISPRCasFinder,CRT matches to KM983327 (Clostridium phage phiCT453A, complete genome) position: , mismatch: 5, identity: 0.861

tgacttctctatttagggaattaatgattaagtata	CRISPR spacer
ctacttctctatttagggaattaatgcttaaataca	Protospacer
. ************************ ****.**.*

17. spacer 4.5|2563609|35|NC_012563|CRISPRCasFinder,CRT,PILER-CR matches to MH319752 (Marine virus AG-345-F15 Ga0172271_14 genomic sequence) position: , mismatch: 6, identity: 0.829

tcacaaaagagtaatactaatgttcaacaattagg-	CRISPR spacer
tcacaaaatagtaatactaaggttcca-actcaggc	Protospacer
******** *********** **** * * *.*** 

18. spacer 3.6|2460042|44|NC_012563|CRT matches to NZ_CP006909 (Clostridium botulinum CDC_1436 plasmid pCBG, complete sequence) position: , mismatch: 8, identity: 0.818

ttgttcaacagaaacccttgtggatgtggttctaatgtttttca	CRISPR spacer
aaagggaaaagaaacccttgtggatgtggttctaatatttttca	Protospacer
  .   ** ***************************.*******

19. spacer 3.6|2460042|44|NC_012563|CRT matches to NZ_CP013684 (Clostridium botulinum strain AM282 plasmid pRSJ10_1, complete sequence) position: , mismatch: 8, identity: 0.818

ttgttcaacagaaacccttgtggatgtggttctaatgtttttca	CRISPR spacer
aaagggaaaagaaacccttgtggatgtggttctaatatttttca	Protospacer
  .   ** ***************************.*******

20. spacer 3.6|2460042|44|NC_012563|CRT matches to NZ_CP013700 (Clostridium botulinum strain AM1195 plasmid pRSJ11_1, complete sequence) position: , mismatch: 8, identity: 0.818

ttgttcaacagaaacccttgtggatgtggttctaatgtttttca	CRISPR spacer
aaagggaaaagaaacccttgtggatgtggttctaatatttttca	Protospacer
  .   ** ***************************.*******

21. spacer 4.1|2563348|36|NC_012563|CRISPRCasFinder,CRT matches to NC_010180 (Bacillus mycoides KBAB4 plasmid pBWB401, complete sequence) position: , mismatch: 8, identity: 0.778

taacattatagttttagaagattttcaaggttttaa	CRISPR spacer
atagaagatagttttagaaggttttcaaggatttta	Protospacer
  * *  *************.********* *** *

22. spacer 2.4|2456036|35|NC_012563|CRISPRCasFinder,CRT,PILER-CR matches to MN693317 (Marine virus AFVG_25M47, complete genome) position: , mismatch: 9, identity: 0.743

caaaacaataagtaattcataattcaaaaatgagg	CRISPR spacer
caaaacaataagtaatttattattcccgcatgcaa	Protospacer
*****************.** ****  . *** ..

23. spacer 4.7|2563740|35|NC_012563|CRISPRCasFinder,CRT,PILER-CR matches to NC_041878 (Pectobacterium phage CBB, complete genome) position: , mismatch: 9, identity: 0.743

aaaataggtgtagaatatgaaccaattttaactac	CRISPR spacer
cagttaggtgtagaatatgaaccatgtttgccaag	Protospacer
 *. ********************  ***. * * 

24. spacer 2.4|2456036|35|NC_012563|CRISPRCasFinder,CRT,PILER-CR matches to MH586730 (Salmonella phage Solent, complete genome) position: , mismatch: 10, identity: 0.714

caaaacaataagtaattcataattcaaaaatgagg	CRISPR spacer
ttcaataataagttattcataattcaaaaacctct	Protospacer
.  **.******* ****************.    

25. spacer 2.4|2456036|35|NC_012563|CRISPRCasFinder,CRT,PILER-CR matches to NC_047786 (Salmonella phage vB_SenS_Sasha, complete genome) position: , mismatch: 10, identity: 0.714

caaaacaataagtaattcataattcaaaaatgagg	CRISPR spacer
ttcaataataagttattcataattcaaaaacctct	Protospacer
.  **.******* ****************.    

26. spacer 2.4|2456036|35|NC_012563|CRISPRCasFinder,CRT,PILER-CR matches to KY002061 (Salmonella phage vB_SenS_Sergei, complete genome) position: , mismatch: 10, identity: 0.714

caaaacaataagtaattcataattcaaaaatgagg	CRISPR spacer
ttcaataataagttattcataattcaaaaacctct	Protospacer
.  **.******* ****************.    

Region Region Position Protein_number Hit_taxonomy Key_proteins Att_site Prophage annotation
DBSCAN-SWA_1 1685562 : 1722671 64 Clostridium_phage(56.67%) terminase,portal,integrase,head,bacteriocin,protease,plate,tail,capsid attL 1683921:1683937|attR 1701645:1701661
DBSCAN-SWA_2 2060348 : 2144395 82 Clostridium_phage(56.25%) terminase,portal,head,integrase,protease,tail,capsid attL 2102248:2102307|attR 2144535:2144632
DBSCAN-SWA_3 2561915 : 2613317 75 uncultured_Caudovirales_phage(35.29%) terminase,portal,integrase,tail,plate,capsid attL 2588983:2589000|attR 2610398:2610415
DBSCAN-SWA_4 2657572 : 2697336 48 Clostridium_phage(83.33%) portal,terminase,plate,tail NA
DBSCAN-SWA_5 2874599 : 2967887 90 Clostridium_phage(48.39%) terminase,tRNA,portal,bacteriocin,head,integrase,protease,tail,capsid attL 2927985:2928044|attR 2967949:2968028
DBSCAN-SWA_6 3293762 : 3303198 7 Synechococcus_phage(42.86%) NA NA
DBSCAN-SWA_7 3452705 : 3475565 28 Clostridium_phage(81.82%) NA NA
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Acr ID Acr position Acr size Homology with known anti Neighbor HTH/AcRanker Neighbor Aca In prophage Protospacer in prophage
NC_012563.1|WP_012704391.1|1688717_1688879_+|hypothetical-protein 1688717_1688879_+ 53 aa aa NA NA NA 1685562-1722671 yes
NC_012563.1|WP_012705376.1|1689034_1689229_+|hypothetical-protein 1689034_1689229_+ 64 aa aa NA NA NA 1685562-1722671 yes
NC_012563.1|WP_012705140.1|1689244_1689442_+|hypothetical-protein 1689244_1689442_+ 65 aa aa NA NA NA 1685562-1722671 yes