CRISPRimmunity

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Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Self targeting spacer number	Target MGE spacer number	Prophage number
NZ_CP005987	Acidithiobacillus caldus ATCC 51756 plasmid megap mpAca1.1, complete sequence	0 crisprs	Cas14u_CAS-V	0	0	2
NZ_CP005989	Acidithiobacillus caldus ATCC 51756 plasmid pACA1.2, complete sequence	0 crisprs	NA	0	0	0
NZ_CP005986	Acidithiobacillus caldus ATCC 51756 chromosome, complete genome	4 crisprs	DEDDh,csa3,c2c4_V-U1,DinG,cas6e,c2c10_CAS-V-U3,cas3,Cas9_archaeal	1	10	8
NZ_CP005988	Acidithiobacillus caldus ATCC 51756 plasmid pACA1.1, complete sequence	0 crisprs	NA	0	0	0

Results visualization

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CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

10954 : 44455

Ralstonia_phage(16.67%)

transposase

The bacterium proteins that are colored denote the protein is present at specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin' and 'tRNA')

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_169737332.1\|10954_11803_-\|transposase	IS5 family transposase	A0A077K814	Ralstonia_phage	2.2e-59	46.8
WP_040131310.1\|12025_13081_-\|transposase	IS5 family transposase	NA	NA	NA	NA
WP_153801860.1\|13129_14146_-	VWA domain-containing protein	NA	NA	NA	NA
WP_153801861.1\|14239_14920_-	hypothetical protein	NA	NA	NA	NA
WP_040131316.1\|15016_16060_-	aliphatic amidase	NA	NA	NA	NA
WP_040131318.1\|16114_16426_-	DUF190 domain-containing protein	NA	NA	NA	NA
WP_014002536.1\|16507_17725_-\|transposase	ISL3-like element ISAtc2 family transposase	A0A2D1GQC1	Lysinibacillus_phage	6.4e-07	23.2
WP_153801862.1\|19314_20439_-	outer membrane beta-barrel protein	NA	NA	NA	NA
WP_040131320.1\|20747_21779_-	alpha-E domain-containing protein	NA	NA	NA	NA
WP_004868127.1\|21763_23230_-	circularly permuted type 2 ATP-grasp protein	NA	NA	NA	NA
WP_153801863.1\|23389_26101_-	transglutaminase family protein	NA	NA	NA	NA
WP_040131322.1\|27315_28182_-	transglutaminase family protein	NA	NA	NA	NA
WP_051620880.1\|28213_28984_-	peptidase	NA	NA	NA	NA
WP_004867800.1\|29095_30037_-	N-linked glycosylation glycosyltransferase PglG	NA	NA	NA	NA
WP_004868469.1\|30181_31444_-\|transposase	IS256 family transposase	A0A2P1JQX9	Mycobacterium_phage	1.4e-89	45.3
WP_040131324.1\|31852_32617_-	hypothetical protein	NA	NA	NA	NA
WP_040131326.1\|32809_33898_+	PAS domain-containing protein	A0A1B0VMK3	Pseudomonas_phage	1.1e-07	28.0
WP_040131328.1\|33894_35340_+	nitrogen regulation protein NR(I)	NA	NA	NA	NA
WP_004871535.1\|35450_36407_+\|transposase	IS5 family transposase	E5E3P6	Burkholderia_phage	2.3e-76	49.7
WP_004873281.1\|36859_37060_+	hypothetical protein	NA	NA	NA	NA
WP_004873278.1\|37063_37648_+	hypothetical protein	NA	NA	NA	NA
WP_040131434.1\|37681_39091_-	glutamate--ammonia ligase	NA	NA	NA	NA
WP_004872123.1\|39569_39908_+	P-II family nitrogen regulator	NA	NA	NA	NA
WP_040131329.1\|39959_41267_+	ammonium transporter	NA	NA	NA	NA
WP_082179252.1\|41370_41907_+	hypothetical protein	NA	NA	NA	NA
WP_040131331.1\|41944_42700_+	type 1 glutamine amidotransferase	NA	NA	NA	NA
WP_082179306.1\|42633_43026_+	P-II family nitrogen regulator	NA	NA	NA	NA
WP_153801864.1\|43558_44455_-\|transposase	IS5 family transposase	Q38213	Escherichia_phage	3.2e-16	29.1

DBSCAN-SWA_2

65547 : 124644

Leptospira_phage(14.29%)

transposase

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_014002536.1\|65547_66765_+\|transposase	ISL3-like element ISAtc2 family transposase	A0A2D1GQC1	Lysinibacillus_phage	6.4e-07	23.2
WP_040131355.1\|67346_68138_+	membrane protein	NA	NA	NA	NA
WP_004867769.1\|68616_69024_+	DedA family protein	NA	NA	NA	NA
WP_077272805.1\|69683_69965_+	DUF4168 domain-containing protein	NA	NA	NA	NA
WP_004867765.1\|70210_71494_-	Y-family DNA polymerase	I6RSM4	Salmonella_phage	3.7e-98	47.2
WP_004867761.1\|71500_71977_-	translesion error-prone DNA polymerase V autoproteolytic subunit	A0A1W6JNS2	Morganella_phage	1.1e-28	49.2
WP_040131357.1\|71966_72212_-	hypothetical protein	NA	NA	NA	NA
WP_040131360.1\|72304_72985_-	SOS response-associated peptidase	V9IQW7	Stenotrophomonas_phage	3.4e-18	31.3
WP_153801871.1\|73236_74241_+\|transposase	IS5 family transposase	Q38213	Escherichia_phage	2.7e-19	27.4
WP_082179310.1\|74313_74778_+\|transposase	transposase	NA	NA	NA	NA
WP_004869209.1\|74809_75718_+	LysR family transcriptional regulator	NA	NA	NA	NA
WP_004869210.1\|75856_76846_+	hypothetical protein	NA	NA	NA	NA
WP_004867878.1\|79577_80633_-\|transposase	IS5-like element ISAtc5 family transposase	NA	NA	NA	NA
WP_040131447.1\|81062_82019_+	glycosyltransferase family 2 protein	F1C5B0	Cronobacter_phage	3.1e-81	51.2
WP_040131364.1\|82028_83669_+	glycosyltransferase family 39 protein	NA	NA	NA	NA
WP_153801866.1\|83881_84052_+	hypothetical protein	NA	NA	NA	NA
WP_004872622.1\|84947_85994_-\|transposase	IS630 family transposase	S5VXX4	Leptospira_phage	2.1e-38	29.8
WP_014002108.1\|86738_86831_+	K(+)-transporting ATPase subunit F	NA	NA	NA	NA
WP_038471437.1\|86827_88588_+	potassium-transporting ATPase subunit KdpA	NA	NA	NA	NA
WP_051620888.1\|88592_90653_+	potassium-transporting ATPase subunit KdpB	E4ZFI9	Streptococcus_phage	1.6e-34	26.8
WP_004870087.1\|90663_91266_+	potassium-transporting ATPase subunit KdpC	NA	NA	NA	NA
WP_038471440.1\|91511_92681_+	porin	NA	NA	NA	NA
WP_004870092.1\|92782_92977_+	hypothetical protein	NA	NA	NA	NA
WP_077272696.1\|93022_93925_+	glutamate 5-kinase	A0A1X9I5D0	Streptococcus_phage	8.5e-33	36.5
WP_004870098.1\|95432_95882_+	hypothetical protein	NA	NA	NA	NA
WP_004870103.1\|96500_98078_+	potassium-transporting ATPase subunit A	NA	NA	NA	NA
WP_004870104.1\|98122_98869_+	response regulator transcription factor	NA	NA	NA	NA
WP_004868027.1\|99112_99382_+	metal-sensitive transcriptional regulator	NA	NA	NA	NA
WP_004868019.1\|99874_100132_+	hypothetical protein	NA	NA	NA	NA
WP_077272803.1\|100195_100381_+	hypothetical protein	NA	NA	NA	NA
WP_082179318.1\|100531_100984_-	GNAT family N-acetyltransferase	NA	NA	NA	NA
WP_014002140.1\|101599_102499_+\|transposase	IS1595 family transposase	NA	NA	NA	NA
WP_004867997.1\|103143_103455_-	DUF1778 domain-containing protein	NA	NA	NA	NA
WP_051620890.1\|103515_105777_-	DEAD/DEAH box helicase	NA	NA	NA	NA
WP_082179320.1\|105746_106991_-	hypothetical protein	NA	NA	NA	NA
WP_004867740.1\|107037_107610_-	hypothetical protein	NA	NA	NA	NA
WP_040131375.1\|107776_109816_-	ATP-dependent helicase	S5MMD7	Bacillus_phage	2.1e-31	24.0
WP_051620892.1\|110009_110630_-	hypothetical protein	NA	NA	NA	NA
WP_004867732.1\|111027_112362_-	type II toxin-antitoxin system HipA family toxin	NA	NA	NA	NA
WP_014003854.1\|112361_112727_-	helix-turn-helix domain-containing protein	NA	NA	NA	NA
WP_082179322.1\|112862_113429_-	SAM-dependent methyltransferase	NA	NA	NA	NA
WP_040131460.1\|113530_113881_-	hypothetical protein	NA	NA	NA	NA
WP_004867720.1\|113947_114985_-	ribonucleotide-diphosphate reductase subunit beta	K4K678	Caulobacter_phage	1.1e-36	30.3
WP_040131378.1\|115114_116284_-	hypothetical protein	NA	NA	NA	NA
WP_040131380.1\|117458_118454_-	ParB/RepB/Spo0J family partition protein	NA	NA	NA	NA
WP_051620896.1\|118458_119508_-	ParA family protein	A0A240F4U1	Ochrobactrum_phage	9.0e-10	33.1
WP_004867714.1\|119685_120834_-	sulfotransferase family protein	NA	NA	NA	NA
WP_004867711.1\|120895_121582_-	hypothetical protein	NA	NA	NA	NA
WP_040131382.1\|121655_122318_-	hypothetical protein	NA	NA	NA	NA
WP_040131384.1\|122362_122656_-	hypothetical protein	NA	NA	NA	NA
WP_051620898.1\|122636_123599_-\|transposase	transposase	A0A1P8DJG9	Virus_Rctr71	5.5e-22	37.5
WP_040131386.1\|123597_124644_+\|transposase	IS630 family transposase	S5VXX4	Leptospira_phage	1.1e-39	30.4

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

2. NZ_CP005986

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CRISPR-Cas detection and classification

Crispr_ID: NZ_CP005986_1

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

NZ_CP005986_1

1606294-1606528

TypeV-U1

Consensus_repeat	Method
CTTGCAAGTACCAAGCCCCTGCAATGGCTA	CRISPRCasFinder
CTTGCAAGTACCAAGCCCCTGCAATGGC	PILER-CR

3 spacers

c2c4_V-U1

The CRISPR arrays of NZ_CP005986_1

>merge|NZ_CP005986|1|1606294-1606528|CRISPRCasFinder,PILER-CR
TTTGTATGGACGAAGCCCCTGCAACGGCCATAGGGTCTCTGCGCCCGGATTGACGACGAGCCAATCTTGCAAGTACGAAGCCCCTGCAATGGCCTCAGCAGTGTACTGTAATAGCATGAGCGAAACAGAGCACTTGCAAGTACCAAGCCCCTGCAATGGCTAGTGCCACCCGCGCTTCTTGCGAGGTCGCAGCAATGTGGACGCGCTTGCAAGTACCAAGCCCCTGCAATGGCTA

>NZ_CP005986|1|1|1606294-1606528|CRISPRCasFinder
TTTGTATGGACGAAGCCCCTGCAACGGCCA	TAGGGTCTCTGCGCCCGGATTGACGACGAGCCAAT
CTTGCAAGTACGAAGCCCCTGCAATGGCCT	CAGCAGTGTACTGTAATAGCATGAGCGAAACAGAGCA
CTTGCAAGTACCAAGCCCCTGCAATGGCTA	GTGCCACCCGCGCTTCTTGCGAGGTCGCAGCAATGTGGACGCG
CTTGCAAGTACCAAGCCCCTGCAATGGCTA

>NZ_CP005986|1|1|1606359-1606526|PILER-CR
CTTGCAAGTACGAAGCCCCTGCAATGGC	CTCAGCAGTGTACTGTAATAGCATGAGCGAAACAGAGCA
CTTGCAAGTACCAAGCCCCTGCAATGGC	TAGTGCCACCCGCGCTTCTTGCGAGGTCGCAGCAATGTGGACGCG
CTTGCAAGTACCAAGCCCCTGCAATGGC

Protein	Signature genes	Signature genes Name	Protein_function
NZ_CP005986.1\|WP_004868469.1\|1610582_1611845_+\|IS256-family-transposase	unknown	unknown	gnl\|CDD\|307151
NZ_CP005986.1\|WP_004872433.1\|1601171_1601933_-\|DUF669-domain-containing-protein	unknown	unknown	gnl\|CDD\|370676
NZ_CP005986.1\|WP_051620853.1\|1619901_1620882_+\|hypothetical-protein	unknown	unknown	unknown
NZ_CP005986.1\|WP_004872435.1\|1603134_1604166_-\|hypothetical-protein	unknown	unknown	unknown
NZ_CP005986.1\|WP_082179248.1\|1618248_1618569_+\|hypothetical-protein	unknown	unknown	unknown
NZ_CP005986.1\|WP_004872432.1\|1599243_1601052_-\|AAA-family-ATPase	unknown	unknown	gnl\|CDD\|350690
NZ_CP005986.1\|WP_051620751.1\|1597138_1599247_-\|DEAD/DEAH-box-helicase	unknown	unknown	gnl\|CDD\|223989
NZ_CP005986.1\|WP_153801809.1\|1604179_1605961_-\|single-stranded-DNA-specific-exonuclease-RecJ	unknown	unknown	gnl\|CDD\|182944
NZ_CP005986.1\|WP_004872439.1\|1606653_1608045_-\|transposase	c2c4_V-U1	-	unknown
NZ_CP005986.1\|WP_004872443.1\|1614047_1615481_-\|sigma-54-dependent-Fis-family-transcriptional-regulator	unknown	unknown	gnl\|CDD\|225114
NZ_CP005986.1\|WP_038471861.1\|1596744_1597137_-\|hypothetical-protein	unknown	unknown	unknown
NZ_CP005986.1\|WP_038471870.1\|1621144_1621369_+\|hypothetical-protein	unknown	unknown	unknown
NZ_CP005986.1\|WP_004872430.1\|1596211_1596757_-\|hypothetical-protein	unknown	unknown	unknown
NZ_CP005986.1\|WP_051620752.1\|1618970_1619471_+\|hydrogenase-maturation-protease	unknown	unknown	gnl\|CDD\|99874
NZ_CP005986.1\|WP_153801847.1\|1608184_1609204_-\|IS5-family-transposase	unknown	unknown	gnl\|CDD\|377531
NZ_CP005986.1\|WP_004872434.1\|1602031_1603108_-\|AAA-family-ATPase	unknown	unknown	gnl\|CDD\|379213
NZ_CP005986.1\|WP_004872438.1\|1606017_1606221_-\|hypothetical-protein	unknown	unknown	gnl\|CDD\|370418
NZ_CP005986.1\|WP_038471859.1\|1595745_1596144_-\|hypothetical-protein	unknown	unknown	unknown
NZ_CP005986.1\|WP_082179247.1\|1616649_1618140_+\|nickel-dependent-hydrogenase-large-subunit	unknown	unknown	gnl\|CDD\|223451
NZ_CP005986.1\|WP_004872444.1\|1615664_1616627_+\|hydrogenase	unknown	unknown	gnl\|CDD\|224654

Protein	Function_ID	Function_description	E-value
NZ_CP005986.1\|WP_004868469.1\|1610582_1611845_+\|IS256-family-transposase	gnl\|CDD\|307151	pfam00872, Transposase_mut, Transposase, Mutator family.	3.34455e-61
NZ_CP005986.1\|WP_004872444.1\|1615664_1616627_+\|hydrogenase	gnl\|CDD\|224654	COG1740, HyaA, Ni,Fe-hydrogenase I small subunit [Energy production and conversion].	6.47897e-115
NZ_CP005986.1\|WP_082179247.1\|1616649_1618140_+\|nickel-dependent-hydrogenase-large-subunit	gnl\|CDD\|223451	COG0374, HyaB, Ni,Fe-hydrogenase I large subunit [Energy production and conversion].	3.80605e-136
NZ_CP005986.1\|WP_004872432.1\|1599243_1601052_-\|AAA-family-ATPase	gnl\|CDD\|350690	cd17932, DEXQc_UvrD, DEXQD-box helicase domain of UvrD. UvrD is a highly conserved helicase involved in mismatch repair, nucleotide excision repair, and recombinational repair. It plays a critical role in maintaining genomic stability and facilitating DNA lesion repair in many prokaryotic species including Helicobacter pylori and Escherichia coli. UvrD is a member of the DEAD-like helicase superfamily, a diverse family of proteins involved in ATP-dependent RNA or DNA unwinding. This domain contains the ATP-binding region.	5.65812e-12
NZ_CP005986.1\|WP_051620751.1\|1597138_1599247_-\|DEAD/DEAH-box-helicase	gnl\|CDD\|223989	COG1061, SSL2, DNA or RNA helicases of superfamily II [Transcription / DNA replication, recombination, and repair].	1.26464e-58
NZ_CP005986.1\|WP_153801809.1\|1604179_1605961_-\|single-stranded-DNA-specific-exonuclease-RecJ	gnl\|CDD\|182944	PRK11070, PRK11070, single-stranded-DNA-specific exonuclease RecJ.	1.45522e-153
NZ_CP005986.1\|WP_051620752.1\|1618970_1619471_+\|hydrogenase-maturation-protease	gnl\|CDD\|99874	cd06063, H2MP_Cyano-H2up, This group of endopeptidases include HupW enzymes that are specific to the cyanobacterial hydrogenase and are involved in the C-terminal cleavage of the hydrogenase large subunit precursor protein. Cyanobacterial nickel-iron (NiFe)-hydrogenases are found exclusively in the N2-fixing strains and are encoded by hup (hydrogen uptake) genes. These uptake hydrogenases are heterodimers with a large (hupL) and small subunit (hupS) and catalyze the consumption of the H2 produced during N2 fixation. Sequence similarity shows that the putative metal-binding resides are well conserved in this group of hydrogen maturation proteases. This group also includes such proteins as the hydrogenase III from Aquifex aeolicus.	1.8356e-58
NZ_CP005986.1\|WP_004872443.1\|1614047_1615481_-\|sigma-54-dependent-Fis-family-transcriptional-regulator	gnl\|CDD\|225114	COG2204, AtoC, Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains [Signal transduction mechanisms].	3.35164e-138
NZ_CP005986.1\|WP_004872433.1\|1601171_1601933_-\|DUF669-domain-containing-protein	gnl\|CDD\|370676	pfam09770, PAT1, Topoisomerase II-associated protein PAT1. Members of this family are necessary for accurate chromosome transmission during cell division.	1.82396e-06
NZ_CP005986.1\|WP_153801847.1\|1608184_1609204_-\|IS5-family-transposase	gnl\|CDD\|377531	pfam05598, DUF772, Transposase domain (DUF772). This presumed domain is found at the N-terminus of many proteins found in transposons.	3.23552e-21
NZ_CP005986.1\|WP_004872434.1\|1602031_1603108_-\|AAA-family-ATPase	gnl\|CDD\|379213	pfam13479, AAA_24, AAA domain. This AAA domain is found in a wide variety of presumed phage proteins.	3.62056e-37
NZ_CP005986.1\|WP_004872438.1\|1606017_1606221_-\|hypothetical-protein	gnl\|CDD\|370418	pfam09302, XLF, XLF-Cernunnos, XRcc4-like factor, NHEJ component. XLF (also called Cernunnos) is Xrcc4-like-factor, and interacts with the XRCC4-DNA ligase IV complex to promote DNA non-homologous end-joining. It directly interacts with the XRCC4-Ligase IV complex and siRNA-mediated down-regulation of XLF in human cell lines leads to radio-sensitivity and impaired DNA non-homologous end-joining. This family contains Nej1 (non-homologous end-joining factor), and Lif1, ligase-interacting factor. XLF forms one of the components of the NHEJ machinery for DNA non-homologous end-joining.	0.00415697

>NZ_CP005986.1|WP_004872438.1|1606017_1606221_-|hypothetical-protein
MPSSTPEKNREHVNTHRARMREAGYVLLSNVWIPQALHKAIARRAKQENKPIWVIVAEALAKAFSET
>NZ_CP005986.1|WP_153801809.1|1604179_1605961_-|single-stranded-DNA-specific-exonuclease-RecJ
MFGVCFGYGTKGITGGSMYPPLIRRLLQDRGADPDDALDLSTISPPLGPQGFLGMKEAVAWIAYAVRHQWPTVIVGDYDVDGACASAILRGSLSGLLPVTSIVPNRFTEGYGLTPAIVDRIAPETKLVITVDNGVSAHAGVAAAKQRGMTVLVTDHHLPGPLRPPADVIVNPNQPGCPFPWKSTCGTAVAWYLVLALHQTHPHWIGREQVESQIDLVALATVADLVKLERNNRVLVANGLRRIREGKINPGLSGIATMAQIELSTIREQDFAFKIGPRLNAAGRLADMQTGIHLLLSRDAEQVERWSRFLETVNAQRKEVQAQIEQEAVEIVNGMSDSGDPVLCVGSDHWHSGVVGIVAGKLKERFERPAFVLTATGEGDLAQGSGRSLDGWHLRDALARLDTEHPGILQRYGGHAMAAGLSIPKSRFDAFRAGINGVLLEQVPTGALHRQVHCDGPLSAAECTLEMARAIEAAGPWGQGFPEPLFENTFVVESARTMKGGHWKLHLRLEDSSSVPTNLVEAVHFLDGKEVAPEPPPSGLRIRAQYRLQVNRWQGREALQLQIHSAYPAPAPVVALTSESSLTPRCDGASPGA
>NZ_CP005986.1|WP_004872435.1|1603134_1604166_-|hypothetical-protein
MHSSDEIKALVVEHMRSSAILSRAIYEISRAGGAKRPLPIGFRLYAALVPLADRYPRVQDGLLRFLLRETSFSSGVQASLENIGERESRRAVLLWSQDRERLKKTLESLAFFLDGLGFTPPQEAEYTKTVSQAILGASIHGAHLGLGGTAYAYSVRDPGKAWDRRLSETVAAWGQVVKRSGVAGNPIGLPQELREATDLAIAMLAENRIDAHTKTYLQDAVAAIDALEATARDLILHYASEPIIASRKTDCERIGAYISNSIGQPVNAQLLKNVWQVAGNMGDHWAEGSIERGVSRAIQACLIVHGILQDAKTTLLKSLQLPPAPARNSSFRTAPQSAGPSGP
>NZ_CP005986.1|WP_004872434.1|1602031_1603108_-|AAA-family-ATPase
MALKIQQGGQFEPIRMMLYGQNGIGKTTFGAGMPKPFILAVEDGIGVMPVTYHRASHEAYSEIVSDLLEVGAYFQRGEFQSLIIDSVDALQSKIIEQVLREHNKKSLNDFSYGKGYDLFKQKFEEFRNLMERFRYELNANVLLIAQTEARTIPDPINGMRDIFTMRLDKRAAGVLRDWADFVGFAQLKAFPIMDKDGKLLKMQTDGTRILSTAPSASFDAKNRFDLPPELPLSWVAVENAIKEAFKRPFDPARFAQPQNAWSTPPAPVPPAPAPASAGWGASPAVPPAAPGYTAAPSNTAAPVQPAAPSNTAAPGQPTPSVQSSGWPAVGSTGPRPQQPAQPASWPSARPNGSGGPTM
>NZ_CP005986.1|WP_004872433.1|1601171_1601933_-|DUF669-domain-containing-protein
MPYGAPVSTLGINKSSTGVGERIEPGIYEVIIKDIERIALKNGTAGYGAKFHFEILNEGPMKGREVGELLNVEAVSEKNRTISLERLAGLAIATGVPEHAVLNQQTVPIDNLMGRTLSVVLGPQRDDPRYVDVKAYMPVGASQNPENWEKMKYLFQEPAPSQQQAPATQGAWGNAPAQQAPAQQQPVAGGWGGQQPAQAPAQQQPAAGGWGNAPAQQAPAQPYAAQQQPAAGAPNGPQAAAPQRQSTWGAGPS
>NZ_CP005986.1|WP_004872432.1|1599243_1601052_-|AAA-family-ATPase
MQPNPEQMAILQAIYEGHRHIAVNALAGTGKSTTVRFSVDGGPLQGKAVQYVVFNRKNAEEAKQKLPGFVKVDTAHGLAWNGQHPEGGLIRAAYGNRMHGSLYGHLKNLRDRDFLDYVDSLKDVSITKAQAIFLVQDILRNFCQSPDETITKSHIGSDLAEKISLRMIRRGTERFFDTAMDAAVRMSNALFGMMYDKNGTMQVTHDAYLKIWSLGNPRIPADHILFDEAQDASLPMMEGILKQDAQLVFIGDTHQSIYGWRGAVDAMQELKRRYPDTVVLPLQSSYRFGQAVADAGNVFLTALYEREKTPHEFRAFLKGLGNYDSRVELGDRIAENRSPTAYLFRSNAPLVGAAIAGLDRGKKVYMAGDQAKEIVDFLEAACKFYRGEFTTHPELKFFDSFDELKKYTETREGQNLKFLTSMVENAHGDLRREIELLQRSAFCQDADYVLSTMHRSKGLEFAHVMLDAGVQKPFELDDDGNKPKFGDIVPEVWRLLYVACTRPHETLYLNGLTNVLLANVQELPDAVVAAIHNLDRYAVDAQPRAAWESLEFPPVPAPQWRTIPVAEANLRYRLKPLAEPSAQQRDLALSASTPVRENLGPA
>NZ_CP005986.1|WP_051620751.1|1597138_1599247_-|DEAD/DEAH-box-helicase
MSVVLRPYQEQAVSALLADARTGICPVGVIPTGGGKTVVMRELSSRWLRQSPQDYLLLLAHRTRLLTQAQERFAGVPVQVVSAGLGLFERPQPGTVTVASIQTLAGMPEIRDVPWSLVLVDEAHRVGMDNEESGQYRTFLDALAARSDPPIVGMTATPWRIEKGNRHVPIFGEGSIFQKASCWIHPDELIAQGFLLSPTLATVRHPLPATGVQLPHGEIATANVTDYKKTDFALRIDEMVRLTQDRHRVLVFAESLSESAAIAKALELLGESACVLHGEHPESVRNREIEKFRAGDFKYLVNVDVFTEGLDVPEIDAVVLHRRTQSVVRYLQMAGRGLRPGGRGADCLIVDFGQHVERLGPIEAARGRNALPKSWRSRRLRRSLAEAAHTADWIGEPDAVPDFTEAEAGFCVRVQRMQVIPLPVRGNRAFYLLKFWVDDPGGGPKPLSVEQKLHLWDSALQQLLRRFSALSGVKVPGYGTVNKGEAWAEAVQRMTRATTGFLAYQPYAVRMRWSGRSGKWMVDGWQWSGRAKMDISLDEERLLSAWVQRTGSSPDPLDRAVYQLLYRLAHEGVLDVRLDAPGNPERPPSVPQSPTPPPATTVPKPAEANPPLSAASDPWGRPPSAPPSFPPIPAFAQEIVRNAGSAEPVSEGGWPEPSVTDFRDTGKPPSGLAETAWGQPNRLPPEEDAQPSRPALPSQGFG
>NZ_CP005986.1|WP_038471861.1|1596744_1597137_-|hypothetical-protein
MELAILVGFPGPSLAGFLEIYAKNWIRHHPEGTPILLSSDAAWARKVGNVQVVQPETSTWKQLSGKCLLVVGREARARLTPVEIGNCGDWLLLGEDTEELIAWWPALAPHLPALKMGFCKFEIKEPSCRY
>NZ_CP005986.1|WP_004872430.1|1596211_1596757_-|hypothetical-protein
MPLLNTPLSPISMIEHASRIITPLMGLVFLASISIGLWMVVMGGWRIFRFGQLSVEDPKLDFKSVVFSFLIGAALVGMAWLMGSSLLTLTGSPKPSPLAYTGPGGQDVATAIVDILHFLQLLGVIWFAHGLRMMYRLGTNNPRQGEFHGKAWTHLLGGLGLIDVAGVAMALAHLAGTQLPF
>NZ_CP005986.1|WP_038471859.1|1595745_1596144_-|hypothetical-protein
MVKQSWRWVKRVGYAVAVTLMGTSPAWANTGLIQDVKNVTEFFSYTPGLITYGGFGLGTVAIVYTGYMLHKKGEYEQQGRPVTVKHIAFPALASAALFGAPFLTGSVQKTFFNDTQGAKATVGTTSQSVNIP
>NZ_CP005986.1|WP_004872439.1|1606653_1608045_-|transposase
MLEAALHAWQKSMQRGRPPRFAVGAEKSQDTLTIQFSNAGGCAVEDIFTGKRKDFSIQYPKKGFRKRSYTPFVFRLGAATDGIYAQGTVQVDREPPQGARVALVRLVRKVMGPKYRYSLQLLLSLPEPVRVEHFARRKPLLAIHIGWNFDEAGRRLAGLSDNGDALDASILSLPVSIEQDLRRSTEIQAIRDANRDAIVQRLKADLVLPDDLPEEDALRVLWEKLRRLPAQHISANRLHYLAGLLALRDAMPEWFELWRKADKRRWQKQVHLAKSARNRRGTFYRQTALDLARRYEAMVLEMPDLKKAAEKLNELTGEKTDLAKKARAGRVIAALYSLESALHWAACKCGTAVLHLSGEETVKSCALCRGVSMVTDPEDGQMLYCEDCGSTVDRKKNGAANAWKLAKENLEPLVTEYWETVLTKTQEAAQRKVEKAQKMAAGRKARAAARKGVDGAESADSRV
>NZ_CP005986.1|WP_153801847.1|1608184_1609204_-|IS5-family-transposase
MARRAAVNNDLLADEIHVQKANAIGDPLQKIEAVVDFSALAQAVEKIAPRPEQPKGGRPPYPTEVMVRVLVVKRLHGLSDEQTEFQLLDRRSFRRFCGLEHSRNIPDRTTIWSFENRIGVDGVTALFNDLDRQIRAHGLEARAGQIVDATLVPAPKQHFTKEEKTLLEQDAVPADWKPTKRRQKDVDASWTKKHGKSHHGYKFTVSVDRKHKFVRTWIADTAKVHDSQHLEAALDEWNTSAEIYADKGYVGAEREERLREQGYRPQIQRKAKPGKPLSACQERRNQRIAKVRARVEHVFAAITQWGGKKIRTIGQARATFAMGMMVIVYNMRRLAFLGA
>NZ_CP005986.1|WP_004868469.1|1610582_1611845_+|IS256-family-transposase
MQESTGFDGGMGELGLNIEGLLRRSARQLIQQAIEGEVQVLLEEYAAVRMVDGRRAVVRNGYLPEREILTAVGPVPVQVPKVRDRSGSGVVFRSSLVPPYVRKSRTVAAALPWLYLHGVSSGRMHEALSVLLGEEAKGLSPAVLGRLKVEWAQEHAQWQRRSLQGKRYAYWWADGVYTQLRAEDDPRMCLLVIIGVTAEGKKEVVAVTDGLRESKASWLEILRDLRDRGLQEAPLLAIGDGAMGFWAALDEIYPQTRHQRCWVHKTANILNELPKRLQGKAKAALQAIWMADTREAAEKAWQAFVRDYQAKYPRAVAKLEKDRDVLLTFFDFPAEHWRHIRSSNAIESTFATVRQRSSRTKNCVSRATFLGLSYKLIQQAERHWRGIQHPERLRELFAGVTFVDGMPANETRLDPQQDAA
>NZ_CP005986.1|WP_004872443.1|1614047_1615481_-|sigma-54-dependent-Fis-family-transcriptional-regulator
MTPILPLSPVLLLVFPDDQEARNLSLQLSAHYECVVANDAASALQIIATRHVDAVICNEELPDMTGSECLFALRTKQPATLRFLSGMAADSRSIHDALYVAAVYEYLRKPLIPDLVAISLKRAVEYNELASSYRRLSHELLLKSGNINITDSDESNPQEKFSDLFYCSRAMADVCNLAKQAAATDLPVLIYGETGTGKELLARGIHYHSRRQSQAFIAENCSSIPPELLHSELFGHRRGAFTGAISDRLGLFSAADGGTVLLDEIEGMTEHLQASLLRFLQSGEIKPVGSDRAHKADVRVIAVTNVSLEILVDQGKFRKDLYYRLKGIEIKIPPLRERPADILMLAEHFARSFAASLGKKYVGFENAAQEMLRDYAFPGNVRELENEVRRAIALTPNGEPIGPQQLSAAIRNNIEHNRVISTSELSGSLKEQVESLEKTLVSSALSRFHGNQSKAANYLGLSRVGLANKIKRYNIPS
>NZ_CP005986.1|WP_004872444.1|1615664_1616627_+|hydrogenase
MANLLWLQGGACSGNTMSFLNAEEPSACDLVTDFGINVLWQPSLGMELGDNVQKILRDCVSGAIPLDIFVFEGTVVNAPNGTGTWNRFAGRPMKEWVKELAGAAQYVVALGDCATWGGIPATAPNPSDSQGLQFLKKAHGGFLGANYRSKAGLPVINIPGCPAHPDWVTQVLVAVATGRAGELALDDLQRPKTFFKSFTQTGCTRNMHFAYKVSATEFGQRKGCLFYDLGCRGPMTHSPCNRILWNRQSSKTRAGMPCLGCTEPEFPFFDLAPGSVFKTQTVMGVPKDLPAGVDKGAYIKLTAAAKSASPAWAEQDIFVV
>NZ_CP005986.1|WP_082179247.1|1616649_1618140_+|nickel-dependent-hydrogenase-large-subunit
MATAVETLDISPVGRVEGDLDVRVDIRDGVVVEAYTQAELFRGFEVILRDKDPQAGLVVTPRACGICGASHLTCAAWALDTAWKTQVPRNAILARNIGQLSESLQSIPRHHYGLFMIDAVNENYRHSKYYEEAVKRYAPFTGKSYEIGVTISGKPVEIYALFGGQWPHSSYMVPGGVMCSPTLSDITRSWSILEYFRTNWLEPVWLGCSMERYEAIKSYDDFMAWLEERPEHANSDLGMYYGIGKDIGLTKFGAGLGKYITWGYLPDEDRYQRPTIETRQQSVYMKAGVYDAKTDKFYPVDQGVTRESTTHGWYDEGADPVHPFNRTTRPMQANNRDFNGAYSWCTEVAHEQLGRLEAGPLSRELIAGGNLGESWQHSDPLVLDMYKKMGPSVWLRHFARMHEACKIYRRIEHCLREFRLDEPFYIKPKEQDGQGWGATEAIRGALAHWIEIKGGKISNYQIIAPTTWNVGPHDARGERGPIEEALIGTPIXRQDR
>NZ_CP005986.1|WP_082179248.1|1618248_1618569_+|hypothetical-protein
MKDNFQNEERIRREIARLLKSGLKTETAPFPEDSVAFAAILSRIRDEAGNDLEKKLIIGGFTNYPVGEDRQRCQECMYYLVHRRWCDLPELDVPVEPDWWCRLWRI
>NZ_CP005986.1|WP_051620752.1|1618970_1619471_+|hydrogenase-maturation-protease
MKRRMVIGCGNLFRRDDGIGIHVIERLRKEQVPNDVELFDAGTSGLEILFQARDCHELIIIDACRSKSEPGAVFKVPGSELPLSYTPTLTLHDFRWDHALYAGTRLFGENFPKSVTAFLIEGSDFGFGDNLSKEVRHALPIVVQQVLAFLVAPQETCNGRLDPSET
>NZ_CP005986.1|WP_051620853.1|1619901_1620882_+|hypothetical-protein
MPETRATTDFNSAIRIARDPRIPAGERAEMLMEIALGLQRRPHEPTDLAYANDLYNEALQICPKDDRLLYGRLLAGQGSALMAIPEGHELLQQALERFQSAEVTLRDLATPEELAELEMNIGLVLQTLAVEGKAHFADAVRRYHSALKIFTAENHPREFAIIQNNIATAYLSMTMGEEAGKMREALAVQAYEAALKVLRLEDHPNEYAMLQNNLGNALQYASSSHPVENNLRAVQCYEEALKVRTATDRPLEYANTISNLANALSNLPDDSERPELGQSKNLDRARYLYAEAEAIFREHGEVAKARLVAETLQEIREYMFGADRVH
>NZ_CP005986.1|WP_038471870.1|1621144_1621369_+|hypothetical-protein
MWDMVWNSAGLFLRGKLFKDTRKVLLLLLANIVLAAILTVAVYVATSSFALACIIAGVISGALQPLLFAKLKYA

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Crispr_ID: NZ_CP005986_2

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

NZ_CP005986_2

1800861-1801017

Orphan

Consensus_repeat	Method
GAACTAGAGCCAGAACTAGAGCCAG	CRISPRCasFinder

2 spacers

The CRISPR arrays of NZ_CP005986_2

>merge|NZ_CP005986|2|1800861-1801017|CRISPRCasFinder
CAATTCGCGCACAAACTAGAGCCAGCACCGCCTACAAAGTTTTTTGTCATGAGTTGTCATGTGTTTTCAGAGTCGGCTTCGTGGCGCACGAACTAGAGCCAGAACTAGAGCCAGGGGGGGTCGCCCGGGGCAGGTCGTTTGGCAGAACTAGAGCCAG

>NZ_CP005986|2|2|1800861-1801017|CRISPRCasFinder
CAATTCGCGCACAAACTAGAGCCAG	CACCGCCTACAAAGTTTTTTGTCATGAGTTGTCATGTGTTTTCAGAGTCGGC
TTCGTGGCGCACGAACTAGAGCCAG	AACTAGAGCCAGGGGGGGTCGCCCGGGGCA
GGTCGTTTGGCAGAACTAGAGCCAG

Protein	Signature genes	Signature genes Name	Protein_function
NZ_CP005986.1\|WP_004872782.1\|1788965_1790282_+\|MFS-transporter	unknown	unknown	gnl\|CDD\|225133
NZ_CP005986.1\|WP_038472016.1\|1792219_1793380_-\|efflux-RND-transporter-periplasmic-adaptor-subunit	unknown	unknown	gnl\|CDD\|185090
NZ_CP005986.1\|WP_038472032.1\|1804593_1805202_+\|uracil-DNA-glycosylase	unknown	unknown	gnl\|CDD\|381680
NZ_CP005986.1\|WP_038472013.1\|1788503_1788923_+\|DUF302-domain-containing-protein	unknown	unknown	gnl\|CDD\|269820
NZ_CP005986.1\|WP_082179262.1\|1798926_1799643_-\|type-1-glutamine-amidotransferase	unknown	unknown	gnl\|CDD\|153212
NZ_CP005986.1\|WP_051620766.1\|1804481_1804694_-\|hypothetical-protein	unknown	unknown	unknown
NZ_CP005986.1\|WP_082179264.1\|1803306_1803651_+\|TraR/DksA-C4-type-zinc-finger-protein	unknown	unknown	gnl\|CDD\|274123
NZ_CP005986.1\|WP_038472035.1\|1805268_1805628_+\|hypothetical-protein	unknown	unknown	gnl\|CDD\|234750
NZ_CP005986.1\|WP_038472022.1\|1796442_1797339_+\|LysR-family-transcriptional-regulator	unknown	unknown	gnl\|CDD\|223656
NZ_CP005986.1\|WP_004872796.1\|1801373_1802315_+\|GTP-binding-protein	unknown	unknown	gnl\|CDD\|197226
NZ_CP005986.1\|WP_004872788.1\|1793372_1794755_-\|efflux-transporter-outer-membrane-subunit	unknown	unknown	gnl\|CDD\|273830
NZ_CP005986.1\|WP_038472019.1\|1794793_1796359_-\|MFS-transporter	unknown	unknown	gnl\|CDD\|129794
NZ_CP005986.1\|WP_004872797.1\|1802316_1803084_+\|metallophosphoesterase	unknown	unknown	gnl\|CDD\|277349
NZ_CP005986.1\|WP_004872799.1\|1803696_1804203_+\|single-stranded-DNA-binding-protein	unknown	unknown	gnl\|CDD\|136097
NZ_CP005986.1\|WP_004872814.1\|1806490_1807057_+\|metallophosphoesterase	unknown	unknown	gnl\|CDD\|277349
NZ_CP005986.1\|WP_004872815.1\|1807406_1807817_-\|type-II-toxin-antitoxin-system-HicB-family-antitoxin	unknown	unknown	gnl\|CDD\|224514
NZ_CP005986.1\|WP_038472026.1\|1798654_1798843_+\|hypothetical-protein	unknown	unknown	unknown
NZ_CP005986.1\|WP_004872813.1\|1805776_1806193_+\|hypothetical-protein	unknown	unknown	gnl\|CDD\|371145
NZ_CP005986.1\|WP_038472823.1\|1790296_1790683_-\|DUF488-family-protein	unknown	unknown	gnl\|CDD\|225730
NZ_CP005986.1\|WP_004872785.1\|1790867_1792142_-\|FAD-dependent-oxidoreductase	unknown	unknown	gnl\|CDD\|170080

Protein	Function_ID	Function_description	E-value
NZ_CP005986.1\|WP_004872782.1\|1788965_1790282_+\|MFS-transporter	gnl\|CDD\|225133	COG2223, NarK, Nitrate/nitrite transporter [Inorganic ion transport and metabolism].	1.91597e-52
NZ_CP005986.1\|WP_038472016.1\|1792219_1793380_-\|efflux-RND-transporter-periplasmic-adaptor-subunit	gnl\|CDD\|185090	PRK15136, PRK15136, multidrug efflux MFS transporter periplasmic adaptor subunit EmrA.	1.11186e-79
NZ_CP005986.1\|WP_038472032.1\|1804593_1805202_+\|uracil-DNA-glycosylase	gnl\|CDD\|381680	cd10030, UDG-F4_TTUDGA_SPO1dp_like, Uracil DNA glycosylase family 4, includes Thermotoga maritima TTUDGA, Bacillus phage SPO1 DNA polymerase, and similar proteins. Uracil DNA glycosylase family 4 includes Thermotoga maritima TTUDGA, a robust uracil DNA glycosylase that shares narrow substrate specificity and high catalytic efficiency with family 1, acting on double-stranded and single-stranded uracil-containing DNA. Members of this family possess four conserved cysteine residues required to coordinate the [4Fe-4S] iron-sulfur cluster. This family also includes the N-terminal domain of Bacillus phage SPO1 DNA polymerase. Bacteriophage SPO1 is one of a group of large, lytic, tailed bacteriophages of Bacillus subtilis, and contains hydroxymethyluracil (hmUra) in place of thymine in their DNA. It has been speculated that this UDG domain may help discriminate between hmUra containing SPO1 DNA and thymine-containing host DNA. Uracil-DNA glycosylases (UDGs) initiate repair of uracils in DNA. Uracil in DNA can arise as a result of mis-incorporation of dUMP residues by DNA polymerase or via deamination of cytosine. Uracil in DNA mispaired with guanine is one of the major pro-mutagenic events, causing G:C->A:T mutations. Thus, UDG is an essential enzyme for maintaining the integrity of genetic information. UDGs have been classified into various families on the basis of their substrate specificity, conserved motifs, and structural similarities. Although these families demonstrate different substrate specificities, often the function of one enzyme can be complemented by the other.	1.52202e-40
NZ_CP005986.1\|WP_038472013.1\|1788503_1788923_+\|DUF302-domain-containing-protein	gnl\|CDD\|269820	cd14797, DUF302, Uncharacterized domain family DUF302. These domains are mostly found in bacterial single-domain proteins and have been shown to form homodimers; they may also bind zinc. Also characterized as COG3439.	5.47473e-25
NZ_CP005986.1\|WP_082179262.1\|1798926_1799643_-\|type-1-glutamine-amidotransferase	gnl\|CDD\|153212	cd01741, GATase1_1, Subgroup of proteins having the Type 1 glutamine amidotransferase (GATase1) domain. This group contains a subgroup of proteins having the Type 1 glutamine amidotransferase (GATase1) domain. GATase activity catalyses the transfer of ammonia from the amide side chain of glutamine to an acceptor substrate. Glutamine amidotransferases (GATase) includes the triad family of amidotransferases which have a conserved Cys-His-Glu catalytic triad in the glutaminase active site. In this subgroup this triad is conserved. GATase activity can be found in a range of biosynthetic enzymes, including: glutamine amidotransferase, formylglycinamide ribonucleotide, GMP synthetase , anthranilate synthase component II, glutamine-dependent carbamoyl phosphate synthase, cytidine triphosphate synthetase, gamma-glutamyl hydrolase, imidazole glycerol phosphate synthase and, cobyric acid synthase. Glutamine amidotransferase (GATase) domains can occur either as single polypeptides, as in glutamine amidotransferases, or as domains in a much larger multifunctional synthase protein, such as CPSase.	8.53337e-69
NZ_CP005986.1\|WP_082179264.1\|1803306_1803651_+\|TraR/DksA-C4-type-zinc-finger-protein	gnl\|CDD\|274123	TIGR02419, kDa_protein_in_gpA_5'region, phage/conjugal plasmid C-4 type zinc finger protein, TraR family. Members of this family are putative C4-type zinc finger proteins found almost exclusively in prophage regions, actual phage, or conjugal transfer regions of the Proteobactia. This small protein (about 70 amino acids) appears homologous to but is smaller than DksA (DnaK suppressor protein), found to be critical for regulating transcription of ribosomal RNA. [Mobile and extrachromosomal element functions, Prophage functions].	5.33508e-07
NZ_CP005986.1\|WP_038472035.1\|1805268_1805628_+\|hypothetical-protein	gnl\|CDD\|234750	PRK00409, PRK00409, recombination and DNA strand exchange inhibitor protein; Reviewed.	0.00621728
NZ_CP005986.1\|WP_038472022.1\|1796442_1797339_+\|LysR-family-transcriptional-regulator	gnl\|CDD\|223656	COG0583, LysR, Transcriptional regulator [Transcription].	1.67861e-45
NZ_CP005986.1\|WP_004872796.1\|1801373_1802315_+\|GTP-binding-protein	gnl\|CDD\|197226	cd09128, PLDc_unchar1_2, Putative catalytic domain, repeat 2, of uncharacterized phospholipase D-like proteins. Putative catalytic domain, repeat 2, of uncharacterized phospholipase D (PLD, EC 3.1.4.4)-like proteins. PLD enzymes hydrolyze phospholipid phosphodiester bonds to yield phosphatidic acid and a free polar head group. They can also catalyze transphosphatidylation of phospholipids to acceptor alcohols. Members of this subfamily contain two HKD motifs (H-x-K-x(4)-D, where x represents any amino acid residue) that characterizes the PLD superfamily. The two motifs may be part of the active site and may be involved in phosphatidyl group transfer.	1.59055e-44
NZ_CP005986.1\|WP_004872788.1\|1793372_1794755_-\|efflux-transporter-outer-membrane-subunit	gnl\|CDD\|273830	TIGR01845, Outer_membrane_protein_OprM, efflux transporter, outer membrane factor (OMF) lipoprotein, NodT family. Members of this model comprise a subfamily of the Outer Membrane Factor (TCDB 1.B.17) porins. OMF proteins operate in conjunction with a primary transporter of the RND, MFS, ABC, or PET systems, and a MFP (membrane fusion protein) to tranport substrates across membranes. The complex thus formed allows transport (export) of various solutes (heavy metal cations; drugs, oligosaccharides, proteins, etc.) across the two envelopes of the Gram-negative bacterial cell envelope in a single energy-coupled step. Current data suggest that the OMF (and not the MFP) is largely responsible for the formation of both the trans-outer membrane and trans-periplasmic channels. The roles played by the MFP have yet to be determined. [Cellular processes, Detoxification, Transport and binding proteins, Porins].	6.33045e-70
NZ_CP005986.1\|WP_038472019.1\|1794793_1796359_-\|MFS-transporter	gnl\|CDD\|129794	TIGR00711, Uncharacterized_MFS-type_transporter_YhcA, drug resistance transporter, EmrB/QacA subfamily. This subfamily of drug efflux proteins, a part of the major faciliator family, is predicted to have 14 potential membrane-spanning regions. Members with known activities include EmrB (multiple drug resistance efflux pump) in E. coli, FarB (antibacterial fatty acid resistance) in Neisseria gonorrhoeae, TcmA (tetracenomycin C resistance) in Streptomyces glaucescens, etc. In most cases, the efflux pump is described as having a second component encoded in the same operon, such as EmrA of E. coli. [Cellular processes, Toxin production and resistance, Transport and binding proteins, Other].	1.52373e-54
NZ_CP005986.1\|WP_004872797.1\|1802316_1803084_+\|metallophosphoesterase	gnl\|CDD\|277349	cd07404, MPP_MS158, Microscilla MS158 and related proteins, metallophosphatase domain. MS158 is an uncharacterized Microscilla protein with a metallophosphatase domain. Microscilla proteins MS152, and MS153 are also included in this family. The domain present in members of this family belongs to the metallophosphatase (MPP) superfamily. MPPs are functionally diverse, but all share a conserved domain with an active site consisting of two metal ions (usually manganese, iron, or zinc) coordinated with octahedral geometry by a cage of histidine, aspartate, and asparagine residues. The MPP superfamily includes: Mre11/SbcD-like exonucleases, Dbr1-like RNA lariat debranching enzymes, YfcE-like phosphodiesterases, purple acid phosphatases (PAPs), YbbF-like UDP-2,3-diacylglucosamine hydrolases, and acid sphingomyelinases (ASMases). The conserved domain is a double beta-sheet sandwich with a di-metal active site made up of residues located at the C-terminal side of the sheets. This domain is thought to allow for productive metal coordination.	2.29833e-39
NZ_CP005986.1\|WP_004872799.1\|1803696_1804203_+\|single-stranded-DNA-binding-protein	gnl\|CDD\|136097	PRK06863, PRK06863, single-stranded DNA-binding protein; Provisional.	4.38766e-60
NZ_CP005986.1\|WP_004872814.1\|1806490_1807057_+\|metallophosphoesterase	gnl\|CDD\|277349	cd07404, MPP_MS158, Microscilla MS158 and related proteins, metallophosphatase domain. MS158 is an uncharacterized Microscilla protein with a metallophosphatase domain. Microscilla proteins MS152, and MS153 are also included in this family. The domain present in members of this family belongs to the metallophosphatase (MPP) superfamily. MPPs are functionally diverse, but all share a conserved domain with an active site consisting of two metal ions (usually manganese, iron, or zinc) coordinated with octahedral geometry by a cage of histidine, aspartate, and asparagine residues. The MPP superfamily includes: Mre11/SbcD-like exonucleases, Dbr1-like RNA lariat debranching enzymes, YfcE-like phosphodiesterases, purple acid phosphatases (PAPs), YbbF-like UDP-2,3-diacylglucosamine hydrolases, and acid sphingomyelinases (ASMases). The conserved domain is a double beta-sheet sandwich with a di-metal active site made up of residues located at the C-terminal side of the sheets. This domain is thought to allow for productive metal coordination.	1.38778e-19
NZ_CP005986.1\|WP_004872815.1\|1807406_1807817_-\|type-II-toxin-antitoxin-system-HicB-family-antitoxin	gnl\|CDD\|224514	COG1598, COG1598, Predicted nuclease of the RNAse H fold, HicB family [General function prediction only].	1.0931e-06
NZ_CP005986.1\|WP_004872813.1\|1805776_1806193_+\|hypothetical-protein	gnl\|CDD\|371145	pfam10577, UPF0560, Uncharacterized protein family UPF0560. This family of proteins has no known function.	2.01304e-06
NZ_CP005986.1\|WP_038472823.1\|1790296_1790683_-\|DUF488-family-protein	gnl\|CDD\|225730	COG3189, COG3189, Uncharacterized conserved protein [Function unknown].	7.93412e-39
NZ_CP005986.1\|WP_004872785.1\|1790867_1792142_-\|FAD-dependent-oxidoreductase	gnl\|CDD\|170080	PRK09754, PRK09754, phenylpropionate dioxygenase ferredoxin reductase subunit; Provisional.	1.3863e-46

>NZ_CP005986.1|WP_082179262.1|1798926_1799643_-|type-1-glutamine-amidotransferase
MSNILVLQHHPEEGLGLLEEIFLESDHHPTIHRLDLGVPVPKNSNAYSGLVIMGGPMSANDEDRYPWLSDEMQLIRATIKAEKPTLGHCLGAQLIAKAMGAQVIANPSGPEIGWFPVQKTVAGRRSLWLKGLPESFLLFHWHGETFMLPEGGIPILSSAICANQAFSIGQHCLALQGHPEVTETIVRSWTLAMAEDLKNTGVGVQSVDEITRDLAQKCASLADISRQLYARWVELLTH
>NZ_CP005986.1|WP_038472026.1|1798654_1798843_+|hypothetical-protein
MGFFEPPLSNNAAIVLLVGKLLAEQTEEWQVIRRHMNQECLVQVVGSDILTAQVLINDPQTA
>NZ_CP005986.1|WP_038472022.1|1796442_1797339_+|LysR-family-transcriptional-regulator
MAIPTPSLELLETFLVVAECGSVSKAAHILHRSQPAISERLQRLTELVGEPLYYGVGRGVRLTAAGEAYLAPARRLRDLKREWSDMVQRRRQLQEGVLKIAATNTVANYFLPQYLVRFRQDYPEVELQLRGGITDWSQYSLLDWDLFFLEEGTPMTGLPPHYAASPWLEDEILAIFPRGHAFAEKPNLDLADLASQPLVWRETQSGIRQRILQEFRKIGIEPSIRIEVNGVEAMGTAVAAGLGIGFITSTALRHRHDWAVESRRVGDPIHWTLYLVAPKTPYRSHTIARFLECLDPAK
>NZ_CP005986.1|WP_038472019.1|1794793_1796359_-|MFS-transporter
MFRILHRQLRSVSAHSVPSRFLAVNLAIGLEHFLVLFNGGAYLPMIPRVIGSLGRNMIYGDWTQDLYFLALGLGLPLGRWASQRFGNRNSLLFCLAAIFFASLVNATTREYVPFLAARIVAGLAGGLSIPLSLNLLLRHYHSDRRDHGLFLWGIAAITPFAIGPFIGGWIDDAWGWRWLFLLNLPILLMSFLGIWLWEPPERQEERPSMDWLGYALLVASLVTLQMLCNLGSKDDWWRSPEIRLLAVLTLVLGGATVWWLRRQTLPALDLRSFRYVNFRVAALGVFLTALVFQGTLALLIVQYQLSFGYSARAIGEILLSLAIFAPASATFSHWYLGRHDPRPLALISLLLLSGAAWWLSSYDLPVSPSSLVEAPMLVGLGLGGAFAAWARIGLWGLSGPSEQRAAVALNLLRSTGQALGIPLVAALWERRLDLHRHFLVEVQGSNLLFWHAAERHFANVPGLLAASVRQHAAMLAFNEIFYLAAGIFGGLALWSLLSRVPKEKSSESIAEQTAIAELVEP
>NZ_CP005986.1|WP_004872788.1|1793372_1794755_-|efflux-transporter-outer-membrane-subunit
MVGGCARLPEQLPNNPKDQVDLDIPRPTGIAAAPWPAQEWWRSANDPELNRLVQIAFARNPDLRAAAAKVLLAQADVGAARSRLLPHFDADFALTQQYFSAQGLHLSANGTSNTFGALNPIDIHYHVDLWGQDRDLVRAARGRLAMNQAEAAQARLLVSTAVVVQYFALEGDRRLLQQTEHLHTLQEQAKAVAEGGFQQGLFNASQIHAEDLALAESAQRIAKLQAAIAAEKHALAALCGEGPSMTIGSATITKQALSPALGLPKNLPLRLLARRPDIVAARWAVQAAAAEVGAARAAFYPNINLRLLAGWNSIHLGDLFNPGNFAHAVGPVITLPIFEGGALRAHLRAQNAVFLAAQDRYRSTLLAALRQIADRLSTAQKLARQERAQRHAAAAAQSQWELRQQAWRQGLSDALPSIAAEITCVQVRQRRTAITTARWQNWALLESALGGGYRHTIKDG
>NZ_CP005986.1|WP_038472016.1|1792219_1793380_-|efflux-RND-transporter-periplasmic-adaptor-subunit
MAELSEDLSQTPIRSRRNYLVFGLILALLLMGWAAWWVLVGSRRIATNDAYVEGNVTPVQAQSAGTIRRVYVENTEYVRAGTILASLQGDRSLLALQASEAALGRTVRQLRQEFAAVQETQAKLRAQQAQLTKLRDDLQRYRGAQASGAVATIQIADTTQDVNALQAEIAATAAKLQAAQALVEGTTLQNNPQVRAAVADLERRYIDWARRDLRAPISGFVVQRSAYPGLMIHPGERLFTIVPLDDLWVVANVKETQMAHLRPGQPVRLESYYYGDRVIFHGTVQGLLPGAGSAFAVLPPENATGNYIHIVERVPIRISLRRAEIRRYPLRPGLSMIAHIDIHQEGPKNVLEPITTTPTRGYESRIYAGELKRARRLAQRIIARNS
>NZ_CP005986.1|WP_004872785.1|1790867_1792142_-|FAD-dependent-oxidoreductase
MDRYGDSTQKPRVHKAEVMDEISSWDYVIVGTGPAAAEAAVAIRELDNRGKLLMLGAERQLPYQRPPLSKGLWTGKDTVEELPLRSAAEWDGLSVQIHLGDAVIELDAGARTLTSASGRIYRYERLLLATGGRARQPELAIATLRDRVFTLRNFQDYERLQAAARPGLTVLVVGGGFLGAELAAALSRQEGLKVHHAFAGNAPLAHVLPPTLQKVVLARYGKAGVQLHPQHRLEGLDWEDGSVRAHFTTQAPIACDLLVYALGMESNLELAQQAGLTLDKGGVLVNTELRSSDSRIWAAGDLATYPDPVWQEPCRLEHWDNAEATGRAAGRAMAGRSEPFRHQSLFFSDLFELGFEAVGRCDSQRVLRVAAPPGGGKAVVYYGDGDWVEGVLLWNVWGKADAAREGIAAHRPLMDLHWVETLET
>NZ_CP005986.1|WP_038472823.1|1790296_1790683_-|DUF488-family-protein
MRIWHVYDKPIPAGYRVLVERLWPRGQRKTDLSLVNWARNLAPSTALRQWFHHDPALWEEFRQRYLAELQDRVPEAQALLTAAAGQDLVLLYAAHDQQHNGALVLADFLRGLVQESHDQQHRATKLGN
>NZ_CP005986.1|WP_004872782.1|1788965_1790282_+|MFS-transporter
MAQNDVNIQGSPRSVLAGATIGFFIGAGSVSLYGPTAHIFVQAMHLSPTQIGWLVGMPMLTGALLRIPFGASVDHNGGRVPFLVLLVASLVGIAGLYWMLLTLYPNHLDAHSYPYLLALGALAGSGIATFSVGVGQVSYWYPRKKQGEALAVFGGLGDSSPGFVALILPSVILMTGLWSGYLFSLAMIILGILLYYFLGKNAPYFQYIHHMEKNAAIDHAKANGEELFPTGGVWHSLSLSASHWRTWPLVGLYFTNFGGFLALTVWLPTFWESYYHTKTMTAVILTASFSLLAAFIRVWGGALADREGGEKIAIISLTILLGGAVIMTLSQTFWLTVIGEFLVGAGMGINNGAVFKLVPHYVPDAVGGASGWVGGLGCLGGFAVPPLLGGFVDRFGTQGYPLGYSVYIGLAVVSLLLVWILVRTRDHVAGGHSISPMH
>NZ_CP005986.1|WP_038472013.1|1788503_1788923_+|DUF302-domain-containing-protein
MNDKNVRGLQQWVSSIGPDVLVTRMEAQLEKSRFTLFGRIDHAHAARSAGLFLPFCTVLIFGRPSGGTELMRVFPTLAIDLPSKILVYQREADVSVVAFTDLSYTVERHGLQGDRLLAQVDAFDREVHTIISTVLTDSG
>NZ_CP005986.1|WP_004872796.1|1801373_1802315_+|GTP-binding-protein
MPLYIEPNAGPAPILNIIDSAHHQLDIGVYYLDDRRILAAVRDAVRRGVDVRIMMEPKPYGMKPWQVRKEVRAIEATGAHFRYVPNRFVSHGDRYAFYHAKYCVNGHEAEIGTANFDWSAFHRNREYLYDTTNMTIVRAVQAVFDADWNRQHASAWAHPVLVLSPGTSADQLLRVIEQPGPIDVESEELGPYRPILDALAAKGKDLRMILPATISAEDRRDVAFLEQHGCQARLMPKKPIYMHAKMIVGNHLAFIGSENFTRTSLEDNREMGLLLNGSDLAHLQAQFDSDWAMAGGHPTSGVLGKLKGWFSRF
>NZ_CP005986.1|WP_004872797.1|1802316_1803084_+|metallophosphoesterase
MRIAFASDIHTEIVALDYYRQTVFTKILQTADVVILAGDIATTPDGLRAVAMWLRAKTRAPILFVLGNHEYYQQVFPSALRDYHAALSDVPAAYLLEKSKIRIGGVRFLGCTLWTDFAGGRHLATCEAGMNDFALIVDVETNKPIRAARIAMEHAASVDWLDTRFPDPMPTVVVTHHAPIFLSNHPRFAGSSIAGGFCANLDDRILSWRPKLWIHGHLHDAVEYTIGETTVVSHPWGYPNERGRIPWFRILDLEI
>NZ_CP005986.1|WP_082179264.1|1803306_1803651_+|TraR/DksA-C4-type-zinc-finger-protein
MLQGDEGDIASQFKEAERVAAISRLRLEPQEEPDEDDQGNRYCLDCGEMIPQARVQAVQAVRCVDCASRLERGKPRVGVGGISRYLRVENDGQQTGSSALDDDLHVEDIGIAKL
>NZ_CP005986.1|WP_004872799.1|1803696_1804203_+|single-stranded-DNA-binding-protein
MSGLNKVILLGYLGADPEMRYMPDGTAVANLSIATSESYKDKEGNRQERTEWHRVSLYRRQAEVAGEYLKKGSMAYVEGRLRTRKWTDKEGQERYTTEIVGDRLQLIGGRRDGDGQESVNGGSKTGRTAPKGSGGGANMDVPPDDFDDKIPFAVAADIARRGLRRVRF
>NZ_CP005986.1|WP_051620766.1|1804481_1804694_-|hypothetical-protein
MTSKGRWRIVSDTSGKTICLREAHSAGQRAQKNINASNLEDERVLACLRNPRGKPKAFLADLQKFLGYDA
>NZ_CP005986.1|WP_038472032.1|1804593_1805202_+|uracil-DNA-glycosylase
MFFCARCPALCASRKQIVLPDVSDTMRHLPLLVIGEAPGADEDVQGRGFVGRAGRTLHRLLESFGLVRWYQYGCANIVRCRPEGNRRPTAEEIWNCGDYLLETLERSEPEAILLVGGTATSVFCGSGPLHTHIVDLRRGDHQLNLDLCHPRLKPFFEQRAVRVFAIPHTSPLSWNRNAPDGRKWSEWAKEEVRVLSAVFAKR
>NZ_CP005986.1|WP_038472035.1|1805268_1805628_+|hypothetical-protein
MAEVQVKRRRRTAEERLADLEAKRQQMEAKLREQLAKIDEEKRRLAGSPSLRKAQMENQKRFERAVQEIAPDLDHRHFIAIIADAVESGFDTDAMADRGESLLQEHGKARRGRRPRSAA
>NZ_CP005986.1|WP_004872813.1|1805776_1806193_+|hypothetical-protein
MDIQTVDQQYQQLQQEAQGVMQSLQTLAGKLKTAADGGNQDAREWLLDLKELALNIQQEQQQVMAVMQALHQAMQNQSQPQWQPGYPQTPQQQGYGQPAMQQGQGGGFLSSFLNSGFGRAIELGAGFGIGDDLINSIF
>NZ_CP005986.1|WP_004872814.1|1806490_1807057_+|metallophosphoesterase
MIHPYLRVLSDLHVDVNPPHLVRLAPLTTDAESILVLAGDYGSPKKLADWLKLAAGSFLHTVVILGNHDYWGMSVERAPEKWRESLANHLPESIFSRVTLLERDTVDVAGIRFIGTTLWSDFDEGDPWALRAAQETMQDHKRIRARGGTKRFLPQDALRWHRDSLRWLERSMAEPWDGPKVVLTHHAP
>NZ_CP005986.1|WP_004872815.1|1807406_1807817_-|type-II-toxin-antitoxin-system-HicB-family-antitoxin
MNIAYPYTIEPQPEGGFLVQFADLEEAFTEGETEEQAAFNAAEVLTAVLAQRIEDGDAIPLPQEAQGRPVAVPDAPVQAALLLHFARQGHTLSELARAMGTSWPAVQRLMKPGNPTLKQLERAAAALGKRLVLSLE

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Crispr_ID: NZ_CP005986_3

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

NZ_CP005986_3

1830737-1830949

Unclear

I-C,I-E,II-B:NA

Consensus_repeat	Method
CGTCGTCCCCGCGTGTGCGGGGGAAATCCTAATTGCAGTC	PILER-CR
GTCGTCCCCGCGTACGCGGGGGAAAACCG	CRISPRCasFinder
GTCGTCCCCGCGNTGTGCGGGGGAAATCCT	CRT

3 spacers

cas6e,DinG

The CRISPR arrays of NZ_CP005986_3

>merge|NZ_CP005986|3|1830737-1830949|PILER-CR,CRISPRCasFinder,CRT
GTCGTCCCCGCGTGTGCGGGGGAAATCCGAGGTGCAGTCGAGGCTCCCGCTACCAGCCGACGTCGTCCCCGCGTGTGCGGGGGAAATCCTAATGATGCTGAAAAGTTGGGGCAGGTCTGGTCGTCGTCCCCGCGTGTGCGGGGGAAATCCTCCTTGCAGTCCGCATTTCGCATTTGCTGCGTTTTCGTCCTCGCAATGTGCGGGGGAAGGCTT

>NZ_CP005986|3|2|1830737-1830897|PILER-CR
GTCGTCCCCGCGTGTGCGGGGGAAATCCGAGGTGCAGTCG	AGGCTCCCGCTACCAGCCGAC
GTCGTCCCCGCGTGTGCGGGGGAAATCCTAATGATGCTGA	AAAGTTGGGGCAGGTCTGGTCGTCGTCC
CCGCGTGTGCGGGGGAAATCCTCCTTGCAGTC

>NZ_CP005986|3|3|1830737-1830888|CRISPRCasFinder
GTCGTCCCCGCGTGTGCGGGGGAAATCCG	AGGTGCAGTCGAGGCTCCCGCTACCAGCCGAC
GTCGTCCCCGCGTGTGCGGGGGAAATCCT	AATGATGCTGAAAAGTTGGGGCAGGTCTGGTC
GTCGTCCCCGCGTGTGCGGGGGAAATCCTC

>NZ_CP005986|3|1|1830737-1830949|CRT
GTCGTCCCCGCGTGTGCGGGGGAAATCCG	AGGTGCAGTCGAGGCTCCCGCTACCAGCCGAC
GTCGTCCCCGCGTGTGCGGGGGAAATCCT	AATGATGCTGAAAAGTTGGGGCAGGTCTGGTC
GTCGTCCCCGCGTGTGCGGGGGAAATCCT	CCTTGCAGTCCGCATTTCGCATTTGCTGCGTTT
TCGTCCTCGCAATGTGCGGGGGAAGGCTT

Protein	Signature genes	Signature genes Name	Protein_function
NZ_CP005986.1\|WP_004868469.1\|1834186_1835449_+\|IS256-family-transposase	unknown	unknown	gnl\|CDD\|307151
NZ_CP005986.1\|WP_169737315.1\|1835534_1836695_+\|DEAD/DEAH-box-helicase	unknown	unknown	gnl\|CDD\|350194
NZ_CP005986.1\|WP_004872869.1\|1836968_1837550_+\|lytic-transglycosylase-domain-containing-protein	unknown	unknown	gnl\|CDD\|381613
NZ_CP005986.1\|WP_004872874.1\|1839768_1839972_+\|hypothetical-protein	unknown	unknown	unknown
NZ_CP005986.1\|WP_004872861.1\|1831049_1832024_+\|hypothetical-protein	unknown	unknown	gnl\|CDD\|274435
NZ_CP005986.1\|WP_004872855.1\|1829400_1830234_+\|hypothetical-protein	unknown	unknown	unknown
NZ_CP005986.1\|WP_004872872.1\|1838507_1839713_+\|DNA-polymerase-III-subunit-beta	unknown	unknown	gnl\|CDD\|238082
NZ_CP005986.1\|WP_004872846.1\|1823770_1824661_+\|thioredoxin-fold-domain-containing-protein	unknown	unknown	gnl\|CDD\|183262
NZ_CP005986.1\|WP_004872840.1\|1821052_1821652_+\|hypothetical-protein	unknown	unknown	gnl\|CDD\|273662
NZ_CP005986.1\|WP_004872876.1\|1840058_1840304_+\|hypothetical-protein	unknown	unknown	unknown
NZ_CP005986.1\|WP_004872864.1\|1833758_1834142_+\|AAA-family-ATPase	unknown	unknown	gnl\|CDD\|350690
NZ_CP005986.1\|WP_004872862.1\|1833080_1833548_-\|hypothetical-protein	unknown	unknown	unknown
NZ_CP005986.1\|WP_153801851.1\|1822340_1823765_+\|site-specific-DNA-methyltransferase	unknown	unknown	gnl\|CDD\|223931
NZ_CP005986.1\|WP_004872849.1\|1824948_1825170_+\|superinfection-immunity-protein	unknown	unknown	gnl\|CDD\|379585
NZ_CP005986.1\|WP_004872847.1\|1824681_1824918_+\|hypothetical-protein	unknown	unknown	unknown
NZ_CP005986.1\|WP_004872854.1\|1828717_1829401_+\|hypothetical-protein	unknown	unknown	unknown
NZ_CP005986.1\|WP_004872871.1\|1837569_1838430_+\|metallophosphoesterase	unknown	unknown	gnl\|CDD\|277367
NZ_CP005986.1\|WP_004872853.1\|1826552_1828733_+\|ATP-dependent-DNA-helicase-DinG	DinG	COG1199_DinG_CAS-IV-A	gnl\|CDD\|224120
NZ_CP005986.1\|WP_070114635.1\|1830175_1830631_+\|type-I-E-CRISPR-associated-protein-Cas6/Cse3/CasE	cas6e	cd09727_cas6e_CAS-I-E	gnl\|CDD\|187858
NZ_CP005986.1\|WP_051620770.1\|1825528_1826356_+\|hypothetical-protein	unknown	unknown	unknown

Protein	Function_ID	Function_description	E-value
NZ_CP005986.1\|WP_004868469.1\|1834186_1835449_+\|IS256-family-transposase	gnl\|CDD\|307151	pfam00872, Transposase_mut, Transposase, Mutator family.	3.34455e-61
NZ_CP005986.1\|WP_169737315.1\|1835534_1836695_+\|DEAD/DEAH-box-helicase	gnl\|CDD\|350194	cd18807, SF1_C_UvrD, C-terminal helicase domain of UvrD family helicases. UvrD is a highly conserved helicase involved in mismatch repair, nucleotide excision repair, and recombinational repair. It plays a critical role in maintaining genomic stability and facilitating DNA lesion repair in many prokaryotic species including Helicobacter pylori and Escherichia coli. This family also includes ATP-dependent helicase/nuclease AddA and helicase/nuclease RecBCD subunit RecB, among others. UvrD family helicases are DEAD-like helicases belonging to superfamily (SF)1, a diverse family of proteins involved in ATP-dependent RNA or DNA unwinding. Similar to SF2 helicases, SF1 helicases do not form toroidal structures like SF3-6 helicases. Their helicase core consists of two similar protein domains that resemble the fold of the recombination protein RecA. This model describes the C-terminal domain, also called HelicC.	5.90346e-11
NZ_CP005986.1\|WP_004872869.1\|1836968_1837550_+\|lytic-transglycosylase-domain-containing-protein	gnl\|CDD\|381613	cd16892, LT_VirB1-like, VirB1-like subfamily. This subfamily includes VirB1 protein, one of twelve proteins making up type IV secretion systems (T4SS). T4SS are macromolecular assemblies generally composed of VirB1-11 and VirD4 proteins, and are used by bacteria to transport material across their membranes. VirB1 acts as a lytic transglycosylase (LT), and is important with respect to piercing the peptidoglycan layer in the periplasm. LTs catalyze the cleavage of the beta-1,4-glycosidic bond between N-acetylmuramic acid (MurNAc) and N-acetyl-D-glucosamine (GlcNAc) as do "goose-type" lysozymes. However, in addition to this, they also make a new glycosidic bond with the C6 hydroxyl group of the same muramic acid residue. Proteins similar to this family include the soluble and insoluble membrane-bound LTs in bacteria, the LTs in bacteriophage lambda, as well as the eukaryotic "goose-type" lysozymes (goose egg-white lysozyme; GEWL).	4.68229e-55
NZ_CP005986.1\|WP_004872871.1\|1837569_1838430_+\|metallophosphoesterase	gnl\|CDD\|277367	cd07424, MPP_PrpA_PrpB, PrpA and PrpB, metallophosphatase domain. PrpA and PrpB are bacterial type I serine/threonine and tyrosine phosphatases thought to modulate the expression of proteins that protect the cell upon accumulation of misfolded proteins in the periplasm. The PPP (phosphoprotein phosphatase) family, to which PrpA and PrpB belong, is one of two known protein phosphatase families specific for serine and threonine. This family also includes: PP1, PP2A, PP2B (calcineurin), PP4, PP5, PP6, PP7, Bsu1, RdgC, PrpE, and ApA4 hydrolase. The PPP catalytic domain is defined by three conserved motifs (-GDXHG-, -GDXVDRG- and -GNHE-). The PPP enzyme family is ancient with members found in all eukaryotes, and in most bacterial and archeal genomes. Dephosphorylation of phosphoserines and phosphothreonines on target proteins plays a central role in the regulation of many cellular processes. PPPs belong to the metallophosphatase (MPP) superfamily. MPPs are functionally diverse, but all share a conserved domain with an active site consisting of two metal ions (usually manganese, iron, or zinc) coordinated with octahedral geometry by a cage of histidine, aspartate, and asparagine residues. The MPP superfamily includes: Mre11/SbcD-like exonucleases, Dbr1-like RNA lariat debranching enzymes, YfcE-like phosphodiesterases, purple acid phosphatases (PAPs), YbbF-like UDP-2,3-diacylglucosamine hydrolases, and acid sphingomyelinases (ASMases). The conserved domain is a double beta-sheet sandwich with a di-metal active site made up of residues located at the C-terminal side of the sheets. This domain is thought to allow for productive metal coordination.	5.3227e-34
NZ_CP005986.1\|WP_004872861.1\|1831049_1832024_+\|hypothetical-protein	gnl\|CDD\|274435	TIGR03115, cas7_csf2, CRISPR type IV/AFERR-associated protein Csf2. Members of this family show up near CRISPR repeats in Acidithiobacillus ferrooxidans ATCC 23270, Azoarcus sp. EbN1, and Rhodoferax ferrireducens DSM 15236. In the latter two species, the CRISPR/cas locus is found on a plasmid. This family is one of several characteristic of a type of CRISPR-associated (cas) gene cluster we designate Aferr after A. ferrooxidans, where it is both chromosomal and the only type of cas gene cluster found. The gene is designated csf2 (CRISPR/cas Subtype as in A. ferrooxidans protein 2), as it lies second closest to the repeats.	3.2931e-06
NZ_CP005986.1\|WP_004872872.1\|1838507_1839713_+\|DNA-polymerase-III-subunit-beta	gnl\|CDD\|238082	cd00140, beta_clamp, Beta clamp domain. The beta subunit (processivity factor) of DNA polymerase III holoenzyme, refered to as the beta clamp, forms a ring shaped dimer that encircles dsDNA (sliding clamp) in bacteria. The beta-clamp is structurally similar to the trimeric ring formed by PCNA (found in eukaryotes and archaea) and the processivity factor (found in bacteriophages T4 and RB69). This structural correspondence further substantiates the mechanistic connection between eukaryotic and prokaryotic DNA replication that has been suggested on biochemical grounds. .	1.54808e-32
NZ_CP005986.1\|WP_004872846.1\|1823770_1824661_+\|thioredoxin-fold-domain-containing-protein	gnl\|CDD\|183262	PRK11657, dsbG, disulfide isomerase/thiol-disulfide oxidase; Provisional.	3.0897e-30
NZ_CP005986.1\|WP_004872840.1\|1821052_1821652_+\|hypothetical-protein	gnl\|CDD\|273662	TIGR01509, HAD-SF-IA-v3, haloacid dehalogenase superfamily, subfamily IA, variant 3 with third motif having DD or ED. This model represents part of one structural subfamily of the Haloacid Dehalogenase (HAD) superfamily of aspartate-nucleophile hydrolases. The superfamily is defined by the presence of three short catalytic motifs. The subfamilies are defined based on the location and the observed or predicted fold of a so-called "capping domain", or the absence of such a domain. Subfamily I consists of sequences in which the capping domain is found in between the first and second catalytic motifs. Subfamily II consists of sequences in which the capping domain is found between the second and third motifs. Subfamily III sequences have no capping domain in either of these positions. The Subfamily IA and IB capping domains are predicted by PSI-PRED to consist of an alpha helical bundle. Subfamily I encompasses such a wide region of sequence space (the sequences are highly divergent) that representing it with a single model is impossible, resulting in an overly broad description which allows in many unrelated sequences. Subfamily IA and IB are separated based on an aparrent phylogenetic bifurcation. Subfamily IA is still too broad to model, but cannot be further subdivided into large chunks based on phylogenetic trees. Of the three motifs defining the HAD superfamily, the third has three variant forms: (1) hhhhsDxxx(x)D, (2) hhhhssxxx(x)D and (3) hhhhDDxxx(x)s where _s_ refers to a small amino acid and _h_ to a hydrophobic one. All three of these variants are found in subfamily IA. Individual models were made based on seeds exhibiting only one of the variants each. Variant 3 (this model) is found in the enzymes beta-phosphoglucomutase (TIGR01990) and deoxyglucose-6-phosphatase, while many other enzymes of subfamily IA exhibit this variant as well as variant 1 (TIGR01549). These three variant models were created with the knowledge that there will be overlap among them - this is by design and serves the purpose of eliminating the overlap with models of more distantly related HAD subfamilies caused by an overly broad single model. [Unknown function, Enzymes of unknown specificity].	8.44197e-05
NZ_CP005986.1\|WP_004872864.1\|1833758_1834142_+\|AAA-family-ATPase	gnl\|CDD\|350690	cd17932, DEXQc_UvrD, DEXQD-box helicase domain of UvrD. UvrD is a highly conserved helicase involved in mismatch repair, nucleotide excision repair, and recombinational repair. It plays a critical role in maintaining genomic stability and facilitating DNA lesion repair in many prokaryotic species including Helicobacter pylori and Escherichia coli. UvrD is a member of the DEAD-like helicase superfamily, a diverse family of proteins involved in ATP-dependent RNA or DNA unwinding. This domain contains the ATP-binding region.	5.70924e-09
NZ_CP005986.1\|WP_153801851.1\|1822340_1823765_+\|site-specific-DNA-methyltransferase	gnl\|CDD\|223931	COG0863, COG0863, DNA modification methylase [DNA replication, recombination, and repair].	6.28559e-06
NZ_CP005986.1\|WP_004872849.1\|1824948_1825170_+\|superinfection-immunity-protein	gnl\|CDD\|379585	pfam14373, Imm_superinfect, Superinfection immunity protein. This family includes the E. coli bacteriophage T4 superinfection immunity (imm) protein. When E. coli is sequentially infected with two T-even type bacteriophage the DNA of the superinfecting phage is excluded from the host, into the periplasmic space. The immunity protein plays a role in this process.	5.59262e-13
NZ_CP005986.1\|WP_004872853.1\|1826552_1828733_+\|ATP-dependent-DNA-helicase-DinG	gnl\|CDD\|224120	COG1199, DinG, Rad3-related DNA helicases [Transcription / DNA replication, recombination, and repair].	1.22422e-37
NZ_CP005986.1\|WP_070114635.1\|1830175_1830631_+\|type-I-E-CRISPR-associated-protein-Cas6/Cse3/CasE	gnl\|CDD\|187858	cd09727, Cas6_I-E, CRISPR/Cas system-associated RAMP superfamily protein Cas6e. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and associated Cas proteins comprise a system for heritable host defense by prokaryotic cells against phage and other foreign DNA; Cas6e is an endoribonuclease that generates crRNA; This family is specific for CRISPR/Cas system I-E subtype; Homologous to Cas6 (RAMP superfamily protein); Possesses double RRM/ferredoxin fold; also known as Cse3 family.	4.02517e-09

>NZ_CP005986.1|WP_070114635.1|1830175_1830631_+|type-I-E-CRISPR-associated-protein-Cas6/Cse3/CasE
MKILDGEALHKKAWDIWAGKASVDERDFLFAPYRDAIVFRSRSEPLPATPEVWRMRSLLAPVMRREHRERVVKPREFRGWLASLLERHGWVLRSIEKVESMEMTIRHGRRLTVVDTVFTAQVVDRENADQSYRSGIGRYKAFGCGMLIPQG
>NZ_CP005986.1|WP_004872855.1|1829400_1830234_+|hypothetical-protein
MERTPLQEAVWFFYERLGHRFPAPDWGEQREDVCSICGTEGRGYDDERPNHRIFRCLACTSFYAPTPDHFLTTGKDRSFAGIVKNPWVLDANGRFRSPIGKASRKDDAFVVNARAHGMDFYPAENPFQTLYRGIYDGEFAFPLLFVRLGQNKIESMRMMRLSTSDMLFFWDKSEMRTFDLALVREAGRLPLGEKPWSDWARWGIPEDLAERVRFLKDVRGKAPFRVLTMHGDNRLFLRQVVDREEAKRPRPRRKRGAENEDSGRRSTAQEGLGYLGG
>NZ_CP005986.1|WP_004872854.1|1828717_1829401_+|hypothetical-protein
MHYVLKVRCKTPIAMGKAGFYSGLDAVLLHRLCLWTGGDTDAARERMPLERDGELWACGGGYNPQDALFLSRTAPRRVPVFRLFLRDGGFREMADLSPDELQTQINAFFGQETEATGDAYAVVRNARELHFRFRSTDPERVLYLLRVEPFLGPGHSVGWGEIEHVELEEKGDADALWVYTDPLNRLTRPVPMDWTGVDGQESLSALEAPYWSMPKVPARVPYPVGVR
>NZ_CP005986.1|WP_004872853.1|1826552_1828733_+|ATP-dependent-DNA-helicase-DinG
MSKLFTLDRFAGAGLRLRSLQIRVAQDVDHCLVEPLPEPEAITSRDGEALSCRRSLCGFIEAGTGVGKTIGYLVPLLEYASQHKKRVGISTYTRFQQRQVAKDLALVQQRIFDGLDLQVAIRLGRQNFVSRERVAFWRERLKGTDLPKAWEDFFAWVDRWDESTDPLDTTFLAWREIYPDMPEFSGLAEEDFALREDEPHAWYEAHAKAAQSADVVITNHATLLIHGALKTGLLGDLDAVVVDEADQLISAAQSLLSRRLRASLVVRDLRRQYPDPREFDFAEARQEADAIQDLLRELGETLEYRETLLSEWLARFPEESAALRGHLDRLIPLLPSPSARRNFQRVLAGLDGGETVDVVYSPIRHLPAFHIDDLEPERFVGKLFVAFGEDEVFLPRVVLVSATLSDPMTEKLDFVHQTFGLFRQPFLTSRYEPDRFGSMTFVLPDPRVGAPFLVDDEDLSERMWNPEWLAYVENALGIDDHHKLLLTPSFEDIARWMERRGLEVGKTRLHDGILYHQPDVPGAKLVQRIEQDPDVRVVITPSLWEGVNLRLDGKPWMQDVLISRVPIPPYSPLLQERKIAWRLARQAEQARKKRERGEKAVWRTEEEVRAELVWESAKKALRRLRQGLGRGIRDERDTVRVWMLDPRVLFPTDALNGLLFVAAGLEDGEHLVQAAEKIPAHPALRRWSQSVPARFRGQLSNADIFLRDGTLSSLVESEGALLCTTC
>NZ_CP005986.1|WP_051620770.1|1825528_1826356_+|hypothetical-protein
MKKMLLGSVLMAISTMALAGTASDGISSIVATNNEAGIAVTGNLFNYQEHITPGPSDTESGWTPGFQVKASVMRDLMGLSNVYGQVKFSYNSGGIDYHGAVENLSTGTATPYDTTDDSTQYRILGRLGKGFAITDNAMLTPYVAIGYQHWNRHLVGPYGYTEDYSAVLTGLGVKGQYALTQRLVLGANAEFLAVVDGQMTPNLDGLPLGTANFGTTGEERVGMDADYRLMGGLHLFGNLDYTHYDYTGGNLNYGFYEPFSASNQFAVGAGLAYSF
>NZ_CP005986.1|WP_004872849.1|1824948_1825170_+|superinfection-immunity-protein
MVTLFILVAFLFAAFYFLPTILAYLRKKSNLVAIFVMNLLLGWLFVGWVIALIWALSNDSPTTIVIHSGKEDR
>NZ_CP005986.1|WP_004872847.1|1824681_1824918_+|hypothetical-protein
MMEADRFLDTQIEVLDIARDMLVKEGLPQDDHHLDLALHALVRGVEIGLMDSQLADRIVRFGAAHARQYPSIGRDYPR
>NZ_CP005986.1|WP_004872846.1|1823770_1824661_+|thioredoxin-fold-domain-containing-protein
MLPMSKKMALWFGWTGFVGMGVLAAGIWTVDLLGTAHAQTNPAAAAANYRPLVDKLSAGRATVLSTFAGPDGLTGLVVGPANGAGPKSIAWGIDGENGPVLIPGPVLDSQGKNLSQQAAERHGVIPKPMAGGKLAQAMLAAPGFTVGTKGPLFTVFLDPNCIFCHDFWTKAYPLAKEGKLRFKVVPVGFLKPSSLPKAVTILQSKDPMAAWAQNEVIFNVKTEAGGTTPAKVLDPKVLAEVKANTELLARTGEVATPTIVACLKGDKTPQVFHGMAPGMMADLGRGASIKGDGTCS
>NZ_CP005986.1|WP_153801851.1|1822340_1823765_+|site-specific-DNA-methyltransferase
MMLPIFAEPEAESRYAIRVGDLLDVLPTLPENHFDGVLCDPPYHLTQKSRGGSTRKANEDDPYGRHGVGTDRGFMGETWDGGDIAFRPETWRAVMRVMKPGGYLMAFGGSRTFHRLAVAFEDAGFVLADTLMWLYGNGFPKNLDLSKAMDRHFGMQRPVVGKRKHPTLKNSDLVEEAANAAHGKNFWRREWDVTLPGHADSARWNGYGTALKPAYEPILLCRKPGEKTYAENALKWGCGALNIDGARIPVSESEGERPLREVTCDPDKNGMVYAGRRNAGGGFDGGSRAIGSTSEGRWPANAILDEEAAAMLNTQAGPRKSGAMPKGTLRRARQGNAFGRFPGVATHKDIAASVGNASRFFYVSKVSTKERQAGVTSGEPRYAHGTTLRRIENSEKVGNDHPTLKPIDLTRYLANLILPPSRGDDSRRLLVPFSGAGSEMIGALQAGWDTVLGIEQSEHYAEIARKRLIYWIGG
>NZ_CP005986.1|WP_004872840.1|1821052_1821652_+|hypothetical-protein
MYPTVALDLDGVLLDFEAAWKTCAAWTLRRPVERITDDYALEDRFGLSRREVAKVWDAFHQGNWWERVHPYPEAWDLIEHLSGLGACVWAVTNVDARHLHARALSLEGMIPESRIITLGAKAMPEDRVAILRDLRATAFLDDRLDNVHAALPYVPICSWIHRDYRGFGEPENGVLVIDDLRDFGTVLQDFLYPVLVATA
>NZ_CP005986.1|WP_004872861.1|1831049_1832024_+|hypothetical-protein
MSMLETLDVSGKLLAVSPIAVSPPGAAFSSDDKGAPQRLPSVGKNRYLPASTFRHVLRYGTIRVVQEAMKAQGKSLSLDALLMLDKGFFAQRKKDKGTLPGRSNGKMKAKDVLRMEQEVREKNPLLGLFGVWTIPSMLRVRNAFPIPNAQGQTYTVASGLVRKALEEDLVDALTKEDAEDYLQRLDDWSEADKETTGLKHISERPWEEIVAGVECEWGYQIHRFTPFQSGAVLAGLRYFASDPVIGAHKAVGRGELAMELNASVIRQDILNGPQSAEAGTLTLRRGEFRVDGLLAEHLSEFDRLAKAGFPGMDFHCLPALVEGE
>NZ_CP005986.1|WP_004872862.1|1833080_1833548_-|hypothetical-protein
MEKRSHHEWVHLPYRRGVQMLEENGVRVLGQTSATRSAWAGISHAEGQPLQVYGMLLVEHDQEYLQVHAAFFRRIGQRDKIRFLSRFFQQAFARWVECDHFSLSESPVVHLGHSPKGIMLTRDMDLPTWIRGRLAKPGRAVCRGKDEGTLRRKTA
>NZ_CP005986.1|WP_004872864.1|1833758_1834142_+|AAA-family-ATPase
MQLTDEQQKAVAMAQKMESFKVSALAGTGKTSTLVAIAKAMGNRRGLYLAFNAAIAAEAKQRFRGTQCESRTFHSLAYRECGYKYKNRMHARLTGSFLRRRFGVNELCSNLVYGRSTTSLRRVAHCC
>NZ_CP005986.1|WP_004868469.1|1834186_1835449_+|IS256-family-transposase
MQESTGFDGGMGELGLNIEGLLRRSARQLIQQAIEGEVQVLLEEYAAVRMVDGRRAVVRNGYLPEREILTAVGPVPVQVPKVRDRSGSGVVFRSSLVPPYVRKSRTVAAALPWLYLHGVSSGRMHEALSVLLGEEAKGLSPAVLGRLKVEWAQEHAQWQRRSLQGKRYAYWWADGVYTQLRAEDDPRMCLLVIIGVTAEGKKEVVAVTDGLRESKASWLEILRDLRDRGLQEAPLLAIGDGAMGFWAALDEIYPQTRHQRCWVHKTANILNELPKRLQGKAKAALQAIWMADTREAAEKAWQAFVRDYQAKYPRAVAKLEKDRDVLLTFFDFPAEHWRHIRSSNAIESTFATVRQRSSRTKNCVSRATFLGLSYKLIQQAERHWRGIQHPERLRELFAGVTFVDGMPANETRLDPQQDAA
>NZ_CP005986.1|WP_169737315.1|1835534_1836695_+|DEAD/DEAH-box-helicase
MRTIRAFLRTVDPKIEAQHVRSSDYCRVIDRKFRLALEEYRYMPDGEDRDHLRQYIALLEQHRATCHQIGKEGLSLARRVWDDMEDPKGETPIVHDFYLREYVLSQPDLGRWYRYLLFDEAQDADPLMLFLTQSQKIPVFYVGDAYQQIYEWRGAANAMESLDLPESTLTLSFRFGQAVADDANEVLDLLGSRLYLRGNPEKSSLVRMGKCGELPSAGGNGSRAIVVRTNAEAVQQTLSGLMSGRRVGLCGASSIRSFLKGYQDLIEGNPSGEFVYFQNEQELREFAQTDMGQDLRMLVKLVDEYGIEELERMVERAVDLDRSTGRVDISVTTAHKSKGLEFDRVFVSEELFNPKRLESDEEKRLLYVAKTRAKQVLCNARPLVAA
>NZ_CP005986.1|WP_004872869.1|1836968_1837550_+|lytic-transglycosylase-domain-containing-protein
MLPLDTLIAQCAPFVAPKTMSAIVRVESGGNPLAMWNNTTGQRILPPNRAAAIAYLQGAIAAGQKVDVGIAQVDTENFAAYGLTPENAFDACTNLRVGAKILQSDYARAAARFGPGQVALYHAFEAYNSGHLWGDAHYANTVLRSAGIPVYVQSSGKMVYRPLATVFSWSTAPTVSAASPPRASVSAAYVLKW
>NZ_CP005986.1|WP_004872871.1|1837569_1838430_+|metallophosphoesterase
MGFLTNPKVQRHEVNARGRDFIVGDLHGCRSMLDTMLSHVGFDGAQDRLFSTGDLVDRGPDSEGCLELLREPWFYPVLGNHDAMLIAWIYGGRIDARCRRYEDAFVNNKGWKWVRRFTRSSEFLPLLERVPLVLVVGSTIQDSPSERFHVAHAELLGSSPWSDALLDAPEEEQQRIWSEKRFIAGFDGIGDGIDAVLWGRELRICGDDFPDRDDPALSTTYVGHTITVLRENPPKLLRRASHVFLDTGAYRAEAEPNGRHGLSLWCHREQRGWHFDGKAFSSITIA
>NZ_CP005986.1|WP_004872872.1|1838507_1839713_+|DNA-polymerase-III-subunit-beta
MERQLSIDTLRKIATASAALVKGNSVQPIYDHVLIEDQRVVLGNATTQLEWFLDEEERSFGFAPEAYPARDLERIAKSLPDGALVEFGEQGQIRCGRSRFRLPVYDGAQYPVKAEPLPSAWIRVQTQDLERIFPSLRRCVAKNDTRTYLNGIHWQIRRVGEQEASLLVLEATDGHRAARFSLEYTPPLDSANDDSAASQGDCSPQGDCSPQGLDAILPVEGLSAWMKLLYSQKIAEFSIAQTRSGVIFQGGGLRLWLTSLDGRYPSLDTILAQLEKRKDVLSLSIADFQRALTLATIGVGESQAVTLSTAPDHLLSVAAKGEERQAEDQIPAQWSGSLPLRYSFNVQYLMDFVDSVKETTKGSEDTEDPQMALRFDVSEKDPTSTFAEVPGGVLVVMPIRE
>NZ_CP005986.1|WP_004872874.1|1839768_1839972_+|hypothetical-protein
MKIDIVLEDDLPLSEASRIVRRMASTIGWDVEWKGDNTFQLRRGPAEDEQDGFGRVAPITDGLRASG
>NZ_CP005986.1|WP_004872876.1|1840058_1840304_+|hypothetical-protein
MSWPLFSLFLYAFGTLVVYLLATHQTGTYFGSLLKPKNFSFLYAVLEVLMRMVIGCLSSIFLLLCSTLVEYSCSSLGDVFR

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Crispr_ID: NZ_CP005986_4

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

NZ_CP005986_4

1832065-1833062

Unclear

I-C,I-E,II-B:NA

Consensus_repeat	Method
GTCGTCCCCGCGTACGCGGGGGAAAACCG	CRISPRCasFinder
GTCGTCCCCGCGTNCGCGGGGGAAA	CRT
CGTCCCCGCGTGCGCGGGGGAAAACC	PILER-CR
GTAGTCCCCGCGTATGCGGGGGAGAACCACAA	PILER-CR

16 spacers

cas6e,DinG

The CRISPR arrays of NZ_CP005986_4

>merge|NZ_CP005986|4|1832065-1833062|CRISPRCasFinder,CRT,PILER-CR,PILER-CR
GTCGTCCCCGCGTGCGCGGGGGAAAACCGTGGGCATCTCCCAATCGCGCGCGCAGGTGTGCGTCGTCCCCGCGTGCGCGGGGGAGAACTGGCTTCGAGACCGACGCGACCGTGGTGCAGGCCGTCGTCCCCGCGTGCGCGGGGGAAAACCGGGATCAGCGGCGTACTCATCCACTTGTGGGCCGTCGTCCCCGCGTGCGCGGGGGAAAACCTTACAGTATGGCGCGGAACGCAGCCGGTGGCGTGTCGTCCCCGTGTGCGCGGTGGAAACCTGATCACCGGGACAAAACCGGGCGGCGGTCGTCCCCGCATGTGCGGGGGAAACCCGCTCCGGACTCCGCTCGTACCCGTCATTCACCGGCGTCCCCGCGTGCGCGGGGGAAAACCTCATCGCTATACCAATGCGCAAGGGGCGGACCCGGCGTCCCCGCGTGCGCGGGGGAAAACCCGCCTTGAGCTCCCTGTTCCGCCATGTGGAGGAGTCGTCCCCGCGTACGCGGGGGAAAACCGGGCGCTGCGGTGGGGGATGACATTTCCCAAAAGTCGTCCCCGCGTACGCGGGGGAAAACCGTACGCGGCTCATGCACACCATTCCAGCGATGGGTCGTCCCCGCGTGCGCGGGGGAAAACCGAGAAAGCAGGAGATTGGAGAGCGTTCTCTATTGTCGTCCCCGTGTACGCGGGGGAAAACCCCGAGCCAGCACCCGACTCTTGATAGCGAATCTGTAGTCCCCGCGTATGCGGGGGAGAACCGCAACCGTACCGGACCCCGCGCCGCCCACGCTAGTAGTCCCCGCGTATGCGGGGGAGAACCAGTCGATATCAGCGGAAACGCTTCGGACATTGCGCCGTCCCCGCGTATGCGGAGGAGAACCACAAGCGGCCAGGTCTCCCGTCATGCTCAAGAAGTCGTCCCCGCGTACGCGGGGGAAAACCGTACACCATGGCGCAAATAATCGACTTTCTGGCCGTCGTCCCCGCGTGCGCGGGGGAAAACCG

>NZ_CP005986|4|4|1832065-1833062|CRISPRCasFinder
GTCGTCCCCGCGTGCGCGGGGGAAAACCG	TGGGCATCTCCCAATCGCGCGCGCAGGTGTGC
GTCGTCCCCGCGTGCGCGGGGGAGAACTG	GCTTCGAGACCGACGCGACCGTGGTGCAGGCC
GTCGTCCCCGCGTGCGCGGGGGAAAACCG	GGATCAGCGGCGTACTCATCCACTTGTGGGCC
GTCGTCCCCGCGTGCGCGGGGGAAAACCT	TACAGTATGGCGCGGAACGCAGCCGGTGGCGT
GTCGTCCCCGTGTGCGCGGTGGAAACCTG	ATCACCGGGACAAAACCGGGCGGCG
GTCGTCCCCGCATGTGCGGGGGAAACCCG	CTCCGGACTCCGCTCGTACCCGTCATTCACC
GGCGTCCCCGCGTGCGCGGGGGAAAACCT	CATCGCTATACCAATGCGCAAGGGGCGGACCC
GGCGTCCCCGCGTGCGCGGGGGAAAACCC	GCCTTGAGCTCCCTGTTCCGCCATGTGGAGGA
GTCGTCCCCGCGTACGCGGGGGAAAACCG	GGCGCTGCGGTGGGGGATGACATTTCCCAAAA
GTCGTCCCCGCGTACGCGGGGGAAAACCG	TACGCGGCTCATGCACACCATTCCAGCGATGG
GTCGTCCCCGCGTGCGCGGGGGAAAACCG	AGAAAGCAGGAGATTGGAGAGCGTTCTCTATT
GTCGTCCCCGTGTACGCGGGGGAAAACCC	CGAGCCAGCACCCGACTCTTGATAGCGAATCT
GTAGTCCCCGCGTATGCGGGGGAGAACCG	CAACCGTACCGGACCCCGCGCCGCCCACGCTA
GTAGTCCCCGCGTATGCGGGGGAGAACCA	GTCGATATCAGCGGAAACGCTTCGGACATTGC
GCCGTCCCCGCGTATGCGGAGGAGAACCA	CAAGCGGCCAGGTCTCCCGTCATGCTCAAGAA
GTCGTCCCCGCGTACGCGGGGGAAAACCG	TACACCATGGCGCAAATAATCGACTTTCTGGCC
GTCGTCCCCGCGTGCGCGGGGGAAAACCG

>NZ_CP005986|4|2|1832065-1833058|CRT
GTCGTCCCCGCGTGCGCGGGGGAAA	ACCGTGGGCATCTCCCAATCGCGCGCGCAGGTGTGC
GTCGTCCCCGCGTGCGCGGGGGAGA	ACTGGCTTCGAGACCGACGCGACCGTGGTGCAGGCC
GTCGTCCCCGCGTGCGCGGGGGAAA	ACCGGGATCAGCGGCGTACTCATCCACTTGTGGGCC
GTCGTCCCCGCGTGCGCGGGGGAAA	ACCTTACAGTATGGCGCGGAACGCAGCCGGTGGCGT
GTCGTCCCCGTGTGCGCGGTGGAAA	CCTGATCACCGGGACAAAACCGGGCGGCG
GTCGTCCCCGCATGTGCGGGGGAAA	CCCGCTCCGGACTCCGCTCGTACCCGTCATTCACC
GGCGTCCCCGCGTGCGCGGGGGAAA	ACCTCATCGCTATACCAATGCGCAAGGGGCGGACCC
GGCGTCCCCGCGTGCGCGGGGGAAA	ACCCGCCTTGAGCTCCCTGTTCCGCCATGTGGAGGA
GTCGTCCCCGCGTACGCGGGGGAAA	ACCGGGCGCTGCGGTGGGGGATGACATTTCCCAAAA
GTCGTCCCCGCGTACGCGGGGGAAA	ACCGTACGCGGCTCATGCACACCATTCCAGCGATGG
GTCGTCCCCGCGTGCGCGGGGGAAA	ACCGAGAAAGCAGGAGATTGGAGAGCGTTCTCTATT
GTCGTCCCCGTGTACGCGGGGGAAA	ACCCCGAGCCAGCACCCGACTCTTGATAGCGAATCT
GTAGTCCCCGCGTATGCGGGGGAGA	ACCGCAACCGTACCGGACCCCGCGCCGCCCACGCTA
GTAGTCCCCGCGTATGCGGGGGAGA	ACCAGTCGATATCAGCGGAAACGCTTCGGACATTGC
GCCGTCCCCGCGTATGCGGAGGAGA	ACCACAAGCGGCCAGGTCTCCCGTCATGCTCAAGAA
GTCGTCCCCGCGTACGCGGGGGAAA	ACCGTACACCATGGCGCAAATAATCGACTTTCTGGCC
GTCGTCCCCGCGTGCGCGGGGGAAA

>NZ_CP005986|4|3|1832425-1832694|PILER-CR
CGTCCCCGCGTGCGCGGGGGAAAACC	TCATCGCTATACCAATGCGCAAGGGGCGGACCCGG
CGTCCCCGCGTGCGCGGGGGAAAACC	CGCCTTGAGCTCCCTGTTCCGCCATGTGGAGGAGT
CGTCCCCGCGTACGCGGGGGAAAACC	GGGCGCTGCGGTGGGGGATGACATTTCCCAAAAGT
CGTCCCCGCGTACGCGGGGGAAAACC	GTACGCGGCTCATGCACACCATTCCAGCGATGGGT
CGTCCCCGCGTGCGCGGGGGAAAACCGAGAAAGCAGGAGATTGGAGAGCGTTCTCTATTGTCGTCCCCGTGTACGCGGGGGAAAACCCCGAGCCAGCACCCGACTCTTGATAGCGAATCTGTAGTCCCCGCGTATGCGGGGGAGAACCGCAA	CCGTACCGGACCCCGCGCCGCCCACGCTA
GTAGTCCCCGCGTATGCGGGGGAGAACCAGTC	GATATCAGCGGAAACGCTTCGGACATTGCGCC


>NZ_CP005986|4|4|1832789-1832942|PILER-CR
	TCATCGCTATACCAATGCGCAAGGGGCGGACCCGG
CGTCCCCGCGTGCGCGGGGGAAAACC	CGCCTTGAGCTCCCTGTTCCGCCATGTGGAGGAGT
CGTCCCCGCGTACGCGGGGGAAAACC	GGGCGCTGCGGTGGGGGATGACATTTCCCAAAAGT
CGTCCCCGCGTACGCGGGGGAAAACC	GTACGCGGCTCATGCACACCATTCCAGCGATGGGT
CGTCCCCGCGTGCGCGGGGGAAAACCGAGAAAGCAGGAGATTGGAGAGCGTTCTCTATTGTCGTCCCCGTGTACGCGGGGGAAAACCCCGAGCCAGCACCCGACTCTTGATAGCGAATCTGTAGTCCCCGCGTATGCGGGGGAGAACCGCAA	CCGTACCGGACCCCGCGCCGCCCACGCTA
GTAGTCCCCGCGTATGCGGGGGAGAACCAGTC	GATATCAGCGGAAACGCTTCGGACATTGCGCC
GTCCCCGCGTATGCGGAGGAGAACCACAA

Protein	Signature genes	Signature genes Name	Protein_function
NZ_CP005986.1\|WP_004868469.1\|1834186_1835449_+\|IS256-family-transposase	unknown	unknown	gnl\|CDD\|307151
NZ_CP005986.1\|WP_169737315.1\|1835534_1836695_+\|DEAD/DEAH-box-helicase	unknown	unknown	gnl\|CDD\|350194
NZ_CP005986.1\|WP_004872869.1\|1836968_1837550_+\|lytic-transglycosylase-domain-containing-protein	unknown	unknown	gnl\|CDD\|381613
NZ_CP005986.1\|WP_004872874.1\|1839768_1839972_+\|hypothetical-protein	unknown	unknown	unknown
NZ_CP005986.1\|WP_004872871.1\|1837569_1838430_+\|metallophosphoesterase	unknown	unknown	gnl\|CDD\|277367
NZ_CP005986.1\|WP_004872855.1\|1829400_1830234_+\|hypothetical-protein	unknown	unknown	unknown
NZ_CP005986.1\|WP_004872872.1\|1838507_1839713_+\|DNA-polymerase-III-subunit-beta	unknown	unknown	gnl\|CDD\|238082
NZ_CP005986.1\|WP_153801851.1\|1822340_1823765_+\|site-specific-DNA-methyltransferase	unknown	unknown	gnl\|CDD\|223931
NZ_CP005986.1\|WP_004872846.1\|1823770_1824661_+\|thioredoxin-fold-domain-containing-protein	unknown	unknown	gnl\|CDD\|183262
NZ_CP005986.1\|WP_004872876.1\|1840058_1840304_+\|hypothetical-protein	unknown	unknown	unknown
NZ_CP005986.1\|WP_004872864.1\|1833758_1834142_+\|AAA-family-ATPase	unknown	unknown	gnl\|CDD\|350690
NZ_CP005986.1\|WP_004872862.1\|1833080_1833548_-\|hypothetical-protein	unknown	unknown	unknown
NZ_CP005986.1\|WP_004872878.1\|1840313_1842887_-\|type-IV-secretory-system-conjugative-DNA-transfer-family-protein	unknown	unknown	gnl\|CDD\|237540
NZ_CP005986.1\|WP_004872861.1\|1831049_1832024_+\|hypothetical-protein	unknown	unknown	gnl\|CDD\|274435
NZ_CP005986.1\|WP_004872849.1\|1824948_1825170_+\|superinfection-immunity-protein	unknown	unknown	gnl\|CDD\|379585
NZ_CP005986.1\|WP_004872847.1\|1824681_1824918_+\|hypothetical-protein	unknown	unknown	unknown
NZ_CP005986.1\|WP_004872854.1\|1828717_1829401_+\|hypothetical-protein	unknown	unknown	unknown
NZ_CP005986.1\|WP_004872853.1\|1826552_1828733_+\|ATP-dependent-DNA-helicase-DinG	DinG	COG1199_DinG_CAS-IV-A	gnl\|CDD\|224120
NZ_CP005986.1\|WP_070114635.1\|1830175_1830631_+\|type-I-E-CRISPR-associated-protein-Cas6/Cse3/CasE	cas6e	cd09727_cas6e_CAS-I-E	gnl\|CDD\|187858
NZ_CP005986.1\|WP_051620770.1\|1825528_1826356_+\|hypothetical-protein	unknown	unknown	unknown

Protein	Function_ID	Function_description	E-value
NZ_CP005986.1\|WP_004868469.1\|1834186_1835449_+\|IS256-family-transposase	gnl\|CDD\|307151	pfam00872, Transposase_mut, Transposase, Mutator family.	3.34455e-61
NZ_CP005986.1\|WP_169737315.1\|1835534_1836695_+\|DEAD/DEAH-box-helicase	gnl\|CDD\|350194	cd18807, SF1_C_UvrD, C-terminal helicase domain of UvrD family helicases. UvrD is a highly conserved helicase involved in mismatch repair, nucleotide excision repair, and recombinational repair. It plays a critical role in maintaining genomic stability and facilitating DNA lesion repair in many prokaryotic species including Helicobacter pylori and Escherichia coli. This family also includes ATP-dependent helicase/nuclease AddA and helicase/nuclease RecBCD subunit RecB, among others. UvrD family helicases are DEAD-like helicases belonging to superfamily (SF)1, a diverse family of proteins involved in ATP-dependent RNA or DNA unwinding. Similar to SF2 helicases, SF1 helicases do not form toroidal structures like SF3-6 helicases. Their helicase core consists of two similar protein domains that resemble the fold of the recombination protein RecA. This model describes the C-terminal domain, also called HelicC.	5.90346e-11
NZ_CP005986.1\|WP_004872869.1\|1836968_1837550_+\|lytic-transglycosylase-domain-containing-protein	gnl\|CDD\|381613	cd16892, LT_VirB1-like, VirB1-like subfamily. This subfamily includes VirB1 protein, one of twelve proteins making up type IV secretion systems (T4SS). T4SS are macromolecular assemblies generally composed of VirB1-11 and VirD4 proteins, and are used by bacteria to transport material across their membranes. VirB1 acts as a lytic transglycosylase (LT), and is important with respect to piercing the peptidoglycan layer in the periplasm. LTs catalyze the cleavage of the beta-1,4-glycosidic bond between N-acetylmuramic acid (MurNAc) and N-acetyl-D-glucosamine (GlcNAc) as do "goose-type" lysozymes. However, in addition to this, they also make a new glycosidic bond with the C6 hydroxyl group of the same muramic acid residue. Proteins similar to this family include the soluble and insoluble membrane-bound LTs in bacteria, the LTs in bacteriophage lambda, as well as the eukaryotic "goose-type" lysozymes (goose egg-white lysozyme; GEWL).	4.68229e-55
NZ_CP005986.1\|WP_004872878.1\|1840313_1842887_-\|type-IV-secretory-system-conjugative-DNA-transfer-family-protein	gnl\|CDD\|237540	PRK13880, PRK13880, conjugal transfer coupling protein TraG; Provisional.	0
NZ_CP005986.1\|WP_004872871.1\|1837569_1838430_+\|metallophosphoesterase	gnl\|CDD\|277367	cd07424, MPP_PrpA_PrpB, PrpA and PrpB, metallophosphatase domain. PrpA and PrpB are bacterial type I serine/threonine and tyrosine phosphatases thought to modulate the expression of proteins that protect the cell upon accumulation of misfolded proteins in the periplasm. The PPP (phosphoprotein phosphatase) family, to which PrpA and PrpB belong, is one of two known protein phosphatase families specific for serine and threonine. This family also includes: PP1, PP2A, PP2B (calcineurin), PP4, PP5, PP6, PP7, Bsu1, RdgC, PrpE, and ApA4 hydrolase. The PPP catalytic domain is defined by three conserved motifs (-GDXHG-, -GDXVDRG- and -GNHE-). The PPP enzyme family is ancient with members found in all eukaryotes, and in most bacterial and archeal genomes. Dephosphorylation of phosphoserines and phosphothreonines on target proteins plays a central role in the regulation of many cellular processes. PPPs belong to the metallophosphatase (MPP) superfamily. MPPs are functionally diverse, but all share a conserved domain with an active site consisting of two metal ions (usually manganese, iron, or zinc) coordinated with octahedral geometry by a cage of histidine, aspartate, and asparagine residues. The MPP superfamily includes: Mre11/SbcD-like exonucleases, Dbr1-like RNA lariat debranching enzymes, YfcE-like phosphodiesterases, purple acid phosphatases (PAPs), YbbF-like UDP-2,3-diacylglucosamine hydrolases, and acid sphingomyelinases (ASMases). The conserved domain is a double beta-sheet sandwich with a di-metal active site made up of residues located at the C-terminal side of the sheets. This domain is thought to allow for productive metal coordination.	5.3227e-34
NZ_CP005986.1\|WP_004872861.1\|1831049_1832024_+\|hypothetical-protein	gnl\|CDD\|274435	TIGR03115, cas7_csf2, CRISPR type IV/AFERR-associated protein Csf2. Members of this family show up near CRISPR repeats in Acidithiobacillus ferrooxidans ATCC 23270, Azoarcus sp. EbN1, and Rhodoferax ferrireducens DSM 15236. In the latter two species, the CRISPR/cas locus is found on a plasmid. This family is one of several characteristic of a type of CRISPR-associated (cas) gene cluster we designate Aferr after A. ferrooxidans, where it is both chromosomal and the only type of cas gene cluster found. The gene is designated csf2 (CRISPR/cas Subtype as in A. ferrooxidans protein 2), as it lies second closest to the repeats.	3.2931e-06
NZ_CP005986.1\|WP_004872872.1\|1838507_1839713_+\|DNA-polymerase-III-subunit-beta	gnl\|CDD\|238082	cd00140, beta_clamp, Beta clamp domain. The beta subunit (processivity factor) of DNA polymerase III holoenzyme, refered to as the beta clamp, forms a ring shaped dimer that encircles dsDNA (sliding clamp) in bacteria. The beta-clamp is structurally similar to the trimeric ring formed by PCNA (found in eukaryotes and archaea) and the processivity factor (found in bacteriophages T4 and RB69). This structural correspondence further substantiates the mechanistic connection between eukaryotic and prokaryotic DNA replication that has been suggested on biochemical grounds. .	1.54808e-32
NZ_CP005986.1\|WP_004872846.1\|1823770_1824661_+\|thioredoxin-fold-domain-containing-protein	gnl\|CDD\|183262	PRK11657, dsbG, disulfide isomerase/thiol-disulfide oxidase; Provisional.	3.0897e-30
NZ_CP005986.1\|WP_004872864.1\|1833758_1834142_+\|AAA-family-ATPase	gnl\|CDD\|350690	cd17932, DEXQc_UvrD, DEXQD-box helicase domain of UvrD. UvrD is a highly conserved helicase involved in mismatch repair, nucleotide excision repair, and recombinational repair. It plays a critical role in maintaining genomic stability and facilitating DNA lesion repair in many prokaryotic species including Helicobacter pylori and Escherichia coli. UvrD is a member of the DEAD-like helicase superfamily, a diverse family of proteins involved in ATP-dependent RNA or DNA unwinding. This domain contains the ATP-binding region.	5.70924e-09
NZ_CP005986.1\|WP_153801851.1\|1822340_1823765_+\|site-specific-DNA-methyltransferase	gnl\|CDD\|223931	COG0863, COG0863, DNA modification methylase [DNA replication, recombination, and repair].	6.28559e-06
NZ_CP005986.1\|WP_004872849.1\|1824948_1825170_+\|superinfection-immunity-protein	gnl\|CDD\|379585	pfam14373, Imm_superinfect, Superinfection immunity protein. This family includes the E. coli bacteriophage T4 superinfection immunity (imm) protein. When E. coli is sequentially infected with two T-even type bacteriophage the DNA of the superinfecting phage is excluded from the host, into the periplasmic space. The immunity protein plays a role in this process.	5.59262e-13
NZ_CP005986.1\|WP_004872853.1\|1826552_1828733_+\|ATP-dependent-DNA-helicase-DinG	gnl\|CDD\|224120	COG1199, DinG, Rad3-related DNA helicases [Transcription / DNA replication, recombination, and repair].	1.22422e-37
NZ_CP005986.1\|WP_070114635.1\|1830175_1830631_+\|type-I-E-CRISPR-associated-protein-Cas6/Cse3/CasE	gnl\|CDD\|187858	cd09727, Cas6_I-E, CRISPR/Cas system-associated RAMP superfamily protein Cas6e. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and associated Cas proteins comprise a system for heritable host defense by prokaryotic cells against phage and other foreign DNA; Cas6e is an endoribonuclease that generates crRNA; This family is specific for CRISPR/Cas system I-E subtype; Homologous to Cas6 (RAMP superfamily protein); Possesses double RRM/ferredoxin fold; also known as Cse3 family.	4.02517e-09

>NZ_CP005986.1|WP_004872861.1|1831049_1832024_+|hypothetical-protein
MSMLETLDVSGKLLAVSPIAVSPPGAAFSSDDKGAPQRLPSVGKNRYLPASTFRHVLRYGTIRVVQEAMKAQGKSLSLDALLMLDKGFFAQRKKDKGTLPGRSNGKMKAKDVLRMEQEVREKNPLLGLFGVWTIPSMLRVRNAFPIPNAQGQTYTVASGLVRKALEEDLVDALTKEDAEDYLQRLDDWSEADKETTGLKHISERPWEEIVAGVECEWGYQIHRFTPFQSGAVLAGLRYFASDPVIGAHKAVGRGELAMELNASVIRQDILNGPQSAEAGTLTLRRGEFRVDGLLAEHLSEFDRLAKAGFPGMDFHCLPALVEGE
>NZ_CP005986.1|WP_070114635.1|1830175_1830631_+|type-I-E-CRISPR-associated-protein-Cas6/Cse3/CasE
MKILDGEALHKKAWDIWAGKASVDERDFLFAPYRDAIVFRSRSEPLPATPEVWRMRSLLAPVMRREHRERVVKPREFRGWLASLLERHGWVLRSIEKVESMEMTIRHGRRLTVVDTVFTAQVVDRENADQSYRSGIGRYKAFGCGMLIPQG
>NZ_CP005986.1|WP_004872855.1|1829400_1830234_+|hypothetical-protein
MERTPLQEAVWFFYERLGHRFPAPDWGEQREDVCSICGTEGRGYDDERPNHRIFRCLACTSFYAPTPDHFLTTGKDRSFAGIVKNPWVLDANGRFRSPIGKASRKDDAFVVNARAHGMDFYPAENPFQTLYRGIYDGEFAFPLLFVRLGQNKIESMRMMRLSTSDMLFFWDKSEMRTFDLALVREAGRLPLGEKPWSDWARWGIPEDLAERVRFLKDVRGKAPFRVLTMHGDNRLFLRQVVDREEAKRPRPRRKRGAENEDSGRRSTAQEGLGYLGG
>NZ_CP005986.1|WP_004872854.1|1828717_1829401_+|hypothetical-protein
MHYVLKVRCKTPIAMGKAGFYSGLDAVLLHRLCLWTGGDTDAARERMPLERDGELWACGGGYNPQDALFLSRTAPRRVPVFRLFLRDGGFREMADLSPDELQTQINAFFGQETEATGDAYAVVRNARELHFRFRSTDPERVLYLLRVEPFLGPGHSVGWGEIEHVELEEKGDADALWVYTDPLNRLTRPVPMDWTGVDGQESLSALEAPYWSMPKVPARVPYPVGVR
>NZ_CP005986.1|WP_004872853.1|1826552_1828733_+|ATP-dependent-DNA-helicase-DinG
MSKLFTLDRFAGAGLRLRSLQIRVAQDVDHCLVEPLPEPEAITSRDGEALSCRRSLCGFIEAGTGVGKTIGYLVPLLEYASQHKKRVGISTYTRFQQRQVAKDLALVQQRIFDGLDLQVAIRLGRQNFVSRERVAFWRERLKGTDLPKAWEDFFAWVDRWDESTDPLDTTFLAWREIYPDMPEFSGLAEEDFALREDEPHAWYEAHAKAAQSADVVITNHATLLIHGALKTGLLGDLDAVVVDEADQLISAAQSLLSRRLRASLVVRDLRRQYPDPREFDFAEARQEADAIQDLLRELGETLEYRETLLSEWLARFPEESAALRGHLDRLIPLLPSPSARRNFQRVLAGLDGGETVDVVYSPIRHLPAFHIDDLEPERFVGKLFVAFGEDEVFLPRVVLVSATLSDPMTEKLDFVHQTFGLFRQPFLTSRYEPDRFGSMTFVLPDPRVGAPFLVDDEDLSERMWNPEWLAYVENALGIDDHHKLLLTPSFEDIARWMERRGLEVGKTRLHDGILYHQPDVPGAKLVQRIEQDPDVRVVITPSLWEGVNLRLDGKPWMQDVLISRVPIPPYSPLLQERKIAWRLARQAEQARKKRERGEKAVWRTEEEVRAELVWESAKKALRRLRQGLGRGIRDERDTVRVWMLDPRVLFPTDALNGLLFVAAGLEDGEHLVQAAEKIPAHPALRRWSQSVPARFRGQLSNADIFLRDGTLSSLVESEGALLCTTC
>NZ_CP005986.1|WP_051620770.1|1825528_1826356_+|hypothetical-protein
MKKMLLGSVLMAISTMALAGTASDGISSIVATNNEAGIAVTGNLFNYQEHITPGPSDTESGWTPGFQVKASVMRDLMGLSNVYGQVKFSYNSGGIDYHGAVENLSTGTATPYDTTDDSTQYRILGRLGKGFAITDNAMLTPYVAIGYQHWNRHLVGPYGYTEDYSAVLTGLGVKGQYALTQRLVLGANAEFLAVVDGQMTPNLDGLPLGTANFGTTGEERVGMDADYRLMGGLHLFGNLDYTHYDYTGGNLNYGFYEPFSASNQFAVGAGLAYSF
>NZ_CP005986.1|WP_004872849.1|1824948_1825170_+|superinfection-immunity-protein
MVTLFILVAFLFAAFYFLPTILAYLRKKSNLVAIFVMNLLLGWLFVGWVIALIWALSNDSPTTIVIHSGKEDR
>NZ_CP005986.1|WP_004872847.1|1824681_1824918_+|hypothetical-protein
MMEADRFLDTQIEVLDIARDMLVKEGLPQDDHHLDLALHALVRGVEIGLMDSQLADRIVRFGAAHARQYPSIGRDYPR
>NZ_CP005986.1|WP_004872846.1|1823770_1824661_+|thioredoxin-fold-domain-containing-protein
MLPMSKKMALWFGWTGFVGMGVLAAGIWTVDLLGTAHAQTNPAAAAANYRPLVDKLSAGRATVLSTFAGPDGLTGLVVGPANGAGPKSIAWGIDGENGPVLIPGPVLDSQGKNLSQQAAERHGVIPKPMAGGKLAQAMLAAPGFTVGTKGPLFTVFLDPNCIFCHDFWTKAYPLAKEGKLRFKVVPVGFLKPSSLPKAVTILQSKDPMAAWAQNEVIFNVKTEAGGTTPAKVLDPKVLAEVKANTELLARTGEVATPTIVACLKGDKTPQVFHGMAPGMMADLGRGASIKGDGTCS
>NZ_CP005986.1|WP_153801851.1|1822340_1823765_+|site-specific-DNA-methyltransferase
MMLPIFAEPEAESRYAIRVGDLLDVLPTLPENHFDGVLCDPPYHLTQKSRGGSTRKANEDDPYGRHGVGTDRGFMGETWDGGDIAFRPETWRAVMRVMKPGGYLMAFGGSRTFHRLAVAFEDAGFVLADTLMWLYGNGFPKNLDLSKAMDRHFGMQRPVVGKRKHPTLKNSDLVEEAANAAHGKNFWRREWDVTLPGHADSARWNGYGTALKPAYEPILLCRKPGEKTYAENALKWGCGALNIDGARIPVSESEGERPLREVTCDPDKNGMVYAGRRNAGGGFDGGSRAIGSTSEGRWPANAILDEEAAAMLNTQAGPRKSGAMPKGTLRRARQGNAFGRFPGVATHKDIAASVGNASRFFYVSKVSTKERQAGVTSGEPRYAHGTTLRRIENSEKVGNDHPTLKPIDLTRYLANLILPPSRGDDSRRLLVPFSGAGSEMIGALQAGWDTVLGIEQSEHYAEIARKRLIYWIGG
>NZ_CP005986.1|WP_004872862.1|1833080_1833548_-|hypothetical-protein
MEKRSHHEWVHLPYRRGVQMLEENGVRVLGQTSATRSAWAGISHAEGQPLQVYGMLLVEHDQEYLQVHAAFFRRIGQRDKIRFLSRFFQQAFARWVECDHFSLSESPVVHLGHSPKGIMLTRDMDLPTWIRGRLAKPGRAVCRGKDEGTLRRKTA
>NZ_CP005986.1|WP_004872864.1|1833758_1834142_+|AAA-family-ATPase
MQLTDEQQKAVAMAQKMESFKVSALAGTGKTSTLVAIAKAMGNRRGLYLAFNAAIAAEAKQRFRGTQCESRTFHSLAYRECGYKYKNRMHARLTGSFLRRRFGVNELCSNLVYGRSTTSLRRVAHCC
>NZ_CP005986.1|WP_004868469.1|1834186_1835449_+|IS256-family-transposase
MQESTGFDGGMGELGLNIEGLLRRSARQLIQQAIEGEVQVLLEEYAAVRMVDGRRAVVRNGYLPEREILTAVGPVPVQVPKVRDRSGSGVVFRSSLVPPYVRKSRTVAAALPWLYLHGVSSGRMHEALSVLLGEEAKGLSPAVLGRLKVEWAQEHAQWQRRSLQGKRYAYWWADGVYTQLRAEDDPRMCLLVIIGVTAEGKKEVVAVTDGLRESKASWLEILRDLRDRGLQEAPLLAIGDGAMGFWAALDEIYPQTRHQRCWVHKTANILNELPKRLQGKAKAALQAIWMADTREAAEKAWQAFVRDYQAKYPRAVAKLEKDRDVLLTFFDFPAEHWRHIRSSNAIESTFATVRQRSSRTKNCVSRATFLGLSYKLIQQAERHWRGIQHPERLRELFAGVTFVDGMPANETRLDPQQDAA
>NZ_CP005986.1|WP_169737315.1|1835534_1836695_+|DEAD/DEAH-box-helicase
MRTIRAFLRTVDPKIEAQHVRSSDYCRVIDRKFRLALEEYRYMPDGEDRDHLRQYIALLEQHRATCHQIGKEGLSLARRVWDDMEDPKGETPIVHDFYLREYVLSQPDLGRWYRYLLFDEAQDADPLMLFLTQSQKIPVFYVGDAYQQIYEWRGAANAMESLDLPESTLTLSFRFGQAVADDANEVLDLLGSRLYLRGNPEKSSLVRMGKCGELPSAGGNGSRAIVVRTNAEAVQQTLSGLMSGRRVGLCGASSIRSFLKGYQDLIEGNPSGEFVYFQNEQELREFAQTDMGQDLRMLVKLVDEYGIEELERMVERAVDLDRSTGRVDISVTTAHKSKGLEFDRVFVSEELFNPKRLESDEEKRLLYVAKTRAKQVLCNARPLVAA
>NZ_CP005986.1|WP_004872869.1|1836968_1837550_+|lytic-transglycosylase-domain-containing-protein
MLPLDTLIAQCAPFVAPKTMSAIVRVESGGNPLAMWNNTTGQRILPPNRAAAIAYLQGAIAAGQKVDVGIAQVDTENFAAYGLTPENAFDACTNLRVGAKILQSDYARAAARFGPGQVALYHAFEAYNSGHLWGDAHYANTVLRSAGIPVYVQSSGKMVYRPLATVFSWSTAPTVSAASPPRASVSAAYVLKW
>NZ_CP005986.1|WP_004872871.1|1837569_1838430_+|metallophosphoesterase
MGFLTNPKVQRHEVNARGRDFIVGDLHGCRSMLDTMLSHVGFDGAQDRLFSTGDLVDRGPDSEGCLELLREPWFYPVLGNHDAMLIAWIYGGRIDARCRRYEDAFVNNKGWKWVRRFTRSSEFLPLLERVPLVLVVGSTIQDSPSERFHVAHAELLGSSPWSDALLDAPEEEQQRIWSEKRFIAGFDGIGDGIDAVLWGRELRICGDDFPDRDDPALSTTYVGHTITVLRENPPKLLRRASHVFLDTGAYRAEAEPNGRHGLSLWCHREQRGWHFDGKAFSSITIA
>NZ_CP005986.1|WP_004872872.1|1838507_1839713_+|DNA-polymerase-III-subunit-beta
MERQLSIDTLRKIATASAALVKGNSVQPIYDHVLIEDQRVVLGNATTQLEWFLDEEERSFGFAPEAYPARDLERIAKSLPDGALVEFGEQGQIRCGRSRFRLPVYDGAQYPVKAEPLPSAWIRVQTQDLERIFPSLRRCVAKNDTRTYLNGIHWQIRRVGEQEASLLVLEATDGHRAARFSLEYTPPLDSANDDSAASQGDCSPQGDCSPQGLDAILPVEGLSAWMKLLYSQKIAEFSIAQTRSGVIFQGGGLRLWLTSLDGRYPSLDTILAQLEKRKDVLSLSIADFQRALTLATIGVGESQAVTLSTAPDHLLSVAAKGEERQAEDQIPAQWSGSLPLRYSFNVQYLMDFVDSVKETTKGSEDTEDPQMALRFDVSEKDPTSTFAEVPGGVLVVMPIRE
>NZ_CP005986.1|WP_004872874.1|1839768_1839972_+|hypothetical-protein
MKIDIVLEDDLPLSEASRIVRRMASTIGWDVEWKGDNTFQLRRGPAEDEQDGFGRVAPITDGLRASG
>NZ_CP005986.1|WP_004872876.1|1840058_1840304_+|hypothetical-protein
MSWPLFSLFLYAFGTLVVYLLATHQTGTYFGSLLKPKNFSFLYAVLEVLMRMVIGCLSSIFLLLCSTLVEYSCSSLGDVFR
>NZ_CP005986.1|WP_004872878.1|1840313_1842887_-|type-IV-secretory-system-conjugative-DNA-transfer-family-protein
MASVVHRPEQNILDYRAQQRIRNAPTLGLFATAMTGFFASYLGASLGVHIAIPEIYDPNFFPGYVAKPFLGLPVFSPWELGKMAYAEWQYPMMQKATEIAGAIAAVPTLAMGIWQFVRSQRLWQSQYQTDLHGSAHWASRDEIVKMALLPDKGLHPAKGQKPSKRVCYVGGIPNERDETDYLQHAGAEHIICFAPTRSGKGVGLVLPTLLGGWQESVVVHDIKGENYLLTAGWRKSIGQKILKFNPGFGMAADPLNGMEADNNGDLGTKEQCCHFNPLEEVRVGTPFEVKDVMNIATMIVDPDGKGLNDHWQKTGFALLTSVILHVLYAEPDKTMRGVAAFLNDPELEDVDQAFDKMMDTQHRPSNPVLLKQWTALYGNATVHPAIAQSAKEMKNKAPNEKSGVISTMMSFLSLYRDPIVAEWTEYSDFHITDLQDADAPVSLYLVTSPEDKNRLKPLIRLVINLIASKFTAEDRLIPKDGRMICKGKHPLLLLLDEFPSLGKMDIFQDAIAFFAGYNVKLFLITQSKSQLEDEGRGYGKSGGSVIIENCHVRIFYAPNDVTTAEWLSKMLGKKTVVLENVTQSLEGSVLPSPKGQSRSLNYQARDLLTPDEVLRLRGPIKRGSNIVKAGDMIVMVAGFAPVYGRQILYFKNPTFLERAQIPPPATSDEISARAKDYYAKLLGHDKPTSIESTKPDFDTFEESDYTDTPPAGSSAADGSPADGSPAGPADGSLDPTNSDTAPRSGADGSSAPRSGAPRSGADGTSDTRSGDQNGGGGGGLDTGTILEEGADEDAEDGTLDVEVSDSVTDLTEIRASFIEKLPARLLEASGGADAWMQDTQRLDRRADKSELSGMLKG

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Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity
NZ_CP005986_4	4.6\|1832392\|31\|NZ_CP005986\|CRISPRCasFinder	1832392-1832422	31	NZ_CP005988.1	7663-7693	0	1.0

1. spacer 4.6|1832392|31|NZ_CP005986|CRISPRCasFinder matches to position: 7663-7693, mismatch: 0, identity: 1.0

ctccggactccgctcgtacccgtcattcacc	CRISPR spacer
ctccggactccgctcgtacccgtcattcacc	Protospacer
*******************************

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity
NZ_CP005986_4	4.6\|1832392\|31\|NZ_CP005986\|CRISPRCasFinder	1832392-1832422	31	NZ_CP005988	Acidithiobacillus caldus ATCC 51756 plasmid pACA1.1, complete sequence	7663-7693	0	1.0
NZ_CP005986_4	4.13\|1832818\|32\|NZ_CP005986\|CRISPRCasFinder	1832818-1832849	32	NC_010600	Acidithiobacillus caldus strain MNG plasmid pTcM1, complete sequence	40375-40406	0	1.0
NZ_CP005986_4	4.37\|1832821\|29\|NZ_CP005986\|PILER-CR	1832821-1832849	29	NC_010600	Acidithiobacillus caldus strain MNG plasmid pTcM1, complete sequence	40375-40403	0	1.0
NZ_CP005986_4	4.6\|1832392\|31\|NZ_CP005986\|CRISPRCasFinder	1832392-1832422	31	NC_015854	Acidithiobacillus caldus SM-1 plasmid pLAtc3, complete sequence	15816-15846	1	0.968
NZ_CP005986_4	4.6\|1832392\|31\|NZ_CP005986\|CRISPRCasFinder	1832392-1832422	31	NZ_CP026330	Acidithiobacillus caldus strain MTH-04 plasmid p2, complete sequence	3525-3555	1	0.968
NZ_CP005986_4	4.6\|1832392\|31\|NZ_CP005986\|CRISPRCasFinder	1832392-1832422	31	NC_010600	Acidithiobacillus caldus strain MNG plasmid pTcM1, complete sequence	23971-24001	1	0.968
NZ_CP005986_4	4.2\|1832155\|32\|NZ_CP005986\|CRISPRCasFinder	1832155-1832186	32	NC_015187	Acidiphilium multivorum AIU301 plasmid pACMV2, complete sequence	30048-30079	3	0.906
NZ_CP005986_4	4.2\|1832155\|32\|NZ_CP005986\|CRISPRCasFinder	1832155-1832186	32	NC_009471	Acidiphilium cryptum JF-5 plasmid pACRY05, complete sequence	4062-4093	3	0.906
NZ_CP005986_4	4.22\|1832388\|35\|NZ_CP005986\|CRT	1832388-1832422	35	NZ_CP005988	Acidithiobacillus caldus ATCC 51756 plasmid pACA1.1, complete sequence	7663-7697	3	0.914
NZ_CP005986_4	4.29\|1832814\|36\|NZ_CP005986\|CRT	1832814-1832849	36	NC_010600	Acidithiobacillus caldus strain MNG plasmid pTcM1, complete sequence	40375-40410	3	0.917
NZ_CP005986_4	4.22\|1832388\|35\|NZ_CP005986\|CRT	1832388-1832422	35	NC_015854	Acidithiobacillus caldus SM-1 plasmid pLAtc3, complete sequence	15816-15850	4	0.886
NZ_CP005986_4	4.22\|1832388\|35\|NZ_CP005986\|CRT	1832388-1832422	35	NZ_CP026330	Acidithiobacillus caldus strain MTH-04 plasmid p2, complete sequence	3525-3559	4	0.886
NZ_CP005986_4	4.22\|1832388\|35\|NZ_CP005986\|CRT	1832388-1832422	35	NC_010600	Acidithiobacillus caldus strain MNG plasmid pTcM1, complete sequence	23967-24001	4	0.886
NZ_CP005986_4	4.37\|1832821\|29\|NZ_CP005986\|PILER-CR	1832821-1832849	29	NZ_CP054615	Azospirillum oryzae strain KACC 14407 plasmid unnamed1, complete sequence	184662-184690	4	0.862
NZ_CP005986_4	4.2\|1832155\|32\|NZ_CP005986\|CRISPRCasFinder	1832155-1832186	32	NZ_CP022191	Yangia pacifica strain YSBP01 plasmid unnamed1, complete sequence	133709-133740	5	0.844
NZ_CP005986_4	4.37\|1832821\|29\|NZ_CP005986\|PILER-CR	1832821-1832849	29	NZ_CP021814	Sinorhizobium meliloti strain M270 plasmid psymB, complete sequence	1530620-1530648	5	0.828
NZ_CP005986_3	3.2\|1830838\|28\|NZ_CP005986\|PILER-CR	1830838-1830865	28	NZ_CP026333	Pseudomonas sp. XWY-1 plasmid pXWY, complete sequence	239883-239910	6	0.786
NZ_CP005986_3	3.2\|1830838\|28\|NZ_CP005986\|PILER-CR	1830838-1830865	28	MN310372	Pseudomonas putida strain 150716811 plasmid p716811-VIM, complete sequence	102420-102447	6	0.786
NZ_CP005986_3	3.2\|1830838\|28\|NZ_CP005986\|PILER-CR	1830838-1830865	28	NC_022739	Pseudomonas sp. VLB120 plasmid pSTY, complete sequence	238878-238905	6	0.786
NZ_CP005986_4	4.13\|1832818\|32\|NZ_CP005986\|CRISPRCasFinder	1832818-1832849	32	NZ_CP040720	Rhodococcus pyridinivorans strain YF3 plasmid unnamed1, complete sequence	92909-92940	6	0.812
NZ_CP005986_4	4.18\|1832151\|36\|NZ_CP005986\|CRT	1832151-1832186	36	NC_009471	Acidiphilium cryptum JF-5 plasmid pACRY05, complete sequence	4062-4097	6	0.833
NZ_CP005986_4	4.18\|1832151\|36\|NZ_CP005986\|CRT	1832151-1832186	36	NC_015187	Acidiphilium multivorum AIU301 plasmid pACMV2, complete sequence	30044-30079	6	0.833
NZ_CP005986_4	4.37\|1832821\|29\|NZ_CP005986\|PILER-CR	1832821-1832849	29	NZ_LR594673	Variovorax sp. PBL-E5 plasmid 3	28605-28633	6	0.793
NZ_CP005986_4	4.13\|1832818\|32\|NZ_CP005986\|CRISPRCasFinder	1832818-1832849	32	KY092483	Streptomyces phage Bioscum, complete genome	30951-30982	7	0.781
NZ_CP005986_4	4.13\|1832818\|32\|NZ_CP005986\|CRISPRCasFinder	1832818-1832849	32	MK305888	Streptomyces phage Austintatious, complete genome	28644-28675	7	0.781
NZ_CP005986_4	4.13\|1832818\|32\|NZ_CP005986\|CRISPRCasFinder	1832818-1832849	32	NC_047792	Streptomyces phage Ididsumtinwong, complete genome	30954-30985	7	0.781
NZ_CP005986_4	4.13\|1832818\|32\|NZ_CP005986\|CRISPRCasFinder	1832818-1832849	32	KY092481	Streptomyces phage PapayaSalad, complete genome	31241-31272	7	0.781
NZ_CP005986_4	4.37\|1832821\|29\|NZ_CP005986\|PILER-CR	1832821-1832849	29	NZ_CP040820	Paraoceanicella profunda strain D4M1 plasmid pD4M1B, complete sequence	74349-74377	7	0.759
NZ_CP005986_4	4.37\|1832821\|29\|NZ_CP005986\|PILER-CR	1832821-1832849	29	KY092483	Streptomyces phage Bioscum, complete genome	30954-30982	7	0.759
NZ_CP005986_4	4.37\|1832821\|29\|NZ_CP005986\|PILER-CR	1832821-1832849	29	MK305888	Streptomyces phage Austintatious, complete genome	28647-28675	7	0.759
NZ_CP005986_4	4.37\|1832821\|29\|NZ_CP005986\|PILER-CR	1832821-1832849	29	NC_047792	Streptomyces phage Ididsumtinwong, complete genome	30957-30985	7	0.759
NZ_CP005986_4	4.37\|1832821\|29\|NZ_CP005986\|PILER-CR	1832821-1832849	29	KY092481	Streptomyces phage PapayaSalad, complete genome	31244-31272	7	0.759
NZ_CP005986_4	4.1\|1832094\|32\|NZ_CP005986\|CRISPRCasFinder	1832094-1832125	32	NZ_CP039697	Novosphingobium sp. ABRDHK2 plasmid pABRDHK22, complete sequence	451425-451456	8	0.75
NZ_CP005986_4	4.2\|1832155\|32\|NZ_CP005986\|CRISPRCasFinder	1832155-1832186	32	NZ_CP030074	Streptomyces sp. ZFG47 plasmid unnamed1, complete sequence	307059-307090	8	0.75
NZ_CP005986_4	4.37\|1832821\|29\|NZ_CP005986\|PILER-CR	1832821-1832849	29	NZ_CP049029	Fluviibacterium aquatile strain SC52 plasmid pSC52_1, complete sequence	353617-353645	8	0.724
NZ_CP005986_4	4.2\|1832155\|32\|NZ_CP005986\|CRISPRCasFinder	1832155-1832186	32	NZ_CP044425	Paracoccus pantotrophus strain DSM 2944 plasmid pPAN2, complete sequence	173587-173618	9	0.719
NZ_CP005986_4	4.2\|1832155\|32\|NZ_CP005986\|CRISPRCasFinder	1832155-1832186	32	AM419438	Archaeal BJ1 virus complete genome	3791-3822	9	0.719
NZ_CP005986_4	4.2\|1832155\|32\|NZ_CP005986\|CRISPRCasFinder	1832155-1832186	32	MN855787	Bacteriophage sp. isolate 71, complete genome	4112-4143	9	0.719
NZ_CP005986_4	4.2\|1832155\|32\|NZ_CP005986\|CRISPRCasFinder	1832155-1832186	32	NC_048178	Escherichia phage Sortsne, complete genome	25860-25891	9	0.719
NZ_CP005986_4	4.2\|1832155\|32\|NZ_CP005986\|CRISPRCasFinder	1832155-1832186	32	NC_008695	Archaeal BJ1 virus, complete genome	3791-3822	9	0.719
NZ_CP005986_4	4.37\|1832821\|29\|NZ_CP005986\|PILER-CR	1832821-1832849	29	AB920995	Ralstonia phage RSJ2 DNA, complete genome	20483-20511	9	0.69
NZ_CP005986_3	3.4\|1830827\|32\|NZ_CP005986\|CRISPRCasFinder,CRT	1830827-1830858	32	NZ_CP026333	Pseudomonas sp. XWY-1 plasmid pXWY, complete sequence	239880-239911	10	0.688
NZ_CP005986_3	3.4\|1830827\|32\|NZ_CP005986\|CRISPRCasFinder,CRT	1830827-1830858	32	MN310372	Pseudomonas putida strain 150716811 plasmid p716811-VIM, complete sequence	102417-102448	10	0.688
NZ_CP005986_3	3.4\|1830827\|32\|NZ_CP005986\|CRISPRCasFinder,CRT	1830827-1830858	32	NC_022739	Pseudomonas sp. VLB120 plasmid pSTY, complete sequence	238875-238906	10	0.688
NZ_CP005986_4	4.13\|1832818\|32\|NZ_CP005986\|CRISPRCasFinder	1832818-1832849	32	NZ_CP040820	Paraoceanicella profunda strain D4M1 plasmid pD4M1B, complete sequence	74346-74377	10	0.688

1. spacer 4.6|1832392|31|NZ_CP005986|CRISPRCasFinder matches to NZ_CP005988 (Acidithiobacillus caldus ATCC 51756 plasmid pACA1.1, complete sequence) position: , mismatch: 0, identity: 1.0

ctccggactccgctcgtacccgtcattcacc	CRISPR spacer
ctccggactccgctcgtacccgtcattcacc	Protospacer
*******************************

2. spacer 4.13|1832818|32|NZ_CP005986|CRISPRCasFinder matches to NC_010600 (Acidithiobacillus caldus strain MNG plasmid pTcM1, complete sequence) position: , mismatch: 0, identity: 1.0

caaccgtaccggaccccgcgccgcccacgcta	CRISPR spacer
caaccgtaccggaccccgcgccgcccacgcta	Protospacer
********************************

3. spacer 4.37|1832821|29|NZ_CP005986|PILER-CR matches to NC_010600 (Acidithiobacillus caldus strain MNG plasmid pTcM1, complete sequence) position: , mismatch: 0, identity: 1.0

ccgtaccggaccccgcgccgcccacgcta	CRISPR spacer
ccgtaccggaccccgcgccgcccacgcta	Protospacer
*****************************

4. spacer 4.6|1832392|31|NZ_CP005986|CRISPRCasFinder matches to NC_015854 (Acidithiobacillus caldus SM-1 plasmid pLAtc3, complete sequence) position: , mismatch: 1, identity: 0.968

ctccggactccgctcgtacccgtcattcacc	CRISPR spacer
ctccggactccgctcgtacccatcattcacc	Protospacer
*********************.*********

5. spacer 4.6|1832392|31|NZ_CP005986|CRISPRCasFinder matches to NZ_CP026330 (Acidithiobacillus caldus strain MTH-04 plasmid p2, complete sequence) position: , mismatch: 1, identity: 0.968

ctccggactccgctcgtacccgtcattcacc	CRISPR spacer
ctccggactccgctcgtacccatcattcacc	Protospacer
*********************.*********

6. spacer 4.6|1832392|31|NZ_CP005986|CRISPRCasFinder matches to NC_010600 (Acidithiobacillus caldus strain MNG plasmid pTcM1, complete sequence) position: , mismatch: 1, identity: 0.968

ctccggactccgctcgtacccgtcattcacc	CRISPR spacer
ctccggactccgctcgtacccatcattcacc	Protospacer
*********************.*********

7. spacer 4.2|1832155|32|NZ_CP005986|CRISPRCasFinder matches to NC_015187 (Acidiphilium multivorum AIU301 plasmid pACMV2, complete sequence) position: , mismatch: 3, identity: 0.906

gcttcgagaccgacgcgaccgtggtgcaggcc	CRISPR spacer
gcttcgagaccgtcgcgagcgtggtgcaggca	Protospacer
************ ***** ************

8. spacer 4.2|1832155|32|NZ_CP005986|CRISPRCasFinder matches to NC_009471 (Acidiphilium cryptum JF-5 plasmid pACRY05, complete sequence) position: , mismatch: 3, identity: 0.906

gcttcgagaccgacgcgaccgtggtgcaggcc	CRISPR spacer
gcttcgagaccgtcgcgagcgtggtgcaggca	Protospacer
************ ***** ************

9. spacer 4.22|1832388|35|NZ_CP005986|CRT matches to NZ_CP005988 (Acidithiobacillus caldus ATCC 51756 plasmid pACA1.1, complete sequence) position: , mismatch: 3, identity: 0.914

cccgctccggactccgctcgtacccgtcattcacc	CRISPR spacer
aaagctccggactccgctcgtacccgtcattcacc	Protospacer
   ********************************

10. spacer 4.29|1832814|36|NZ_CP005986|CRT matches to NC_010600 (Acidithiobacillus caldus strain MNG plasmid pTcM1, complete sequence) position: , mismatch: 3, identity: 0.917

accgcaaccgtaccggaccccgcgccgcccacgcta	CRISPR spacer
gatgcaaccgtaccggaccccgcgccgcccacgcta	Protospacer
. .*********************************

11. spacer 4.22|1832388|35|NZ_CP005986|CRT matches to NC_015854 (Acidithiobacillus caldus SM-1 plasmid pLAtc3, complete sequence) position: , mismatch: 4, identity: 0.886

cccgctccggactccgctcgtacccgtcattcacc	CRISPR spacer
aaagctccggactccgctcgtacccatcattcacc	Protospacer
   **********************.*********

12. spacer 4.22|1832388|35|NZ_CP005986|CRT matches to NZ_CP026330 (Acidithiobacillus caldus strain MTH-04 plasmid p2, complete sequence) position: , mismatch: 4, identity: 0.886

cccgctccggactccgctcgtacccgtcattcacc	CRISPR spacer
aaagctccggactccgctcgtacccatcattcacc	Protospacer
   **********************.*********

13. spacer 4.22|1832388|35|NZ_CP005986|CRT matches to NC_010600 (Acidithiobacillus caldus strain MNG plasmid pTcM1, complete sequence) position: , mismatch: 4, identity: 0.886

cccgctccggactccgctcgtacccgtcattcacc	CRISPR spacer
aaagctccggactccgctcgtacccatcattcacc	Protospacer
   **********************.*********

14. spacer 4.37|1832821|29|NZ_CP005986|PILER-CR matches to NZ_CP054615 (Azospirillum oryzae strain KACC 14407 plasmid unnamed1, complete sequence) position: , mismatch: 4, identity: 0.862

ccgtaccggaccccgcgccgc-ccacgcta	CRISPR spacer
ccgtaccggaccccgctccgcgccgccct-	Protospacer
**************** **** **.* **

15. spacer 4.2|1832155|32|NZ_CP005986|CRISPRCasFinder matches to NZ_CP022191 (Yangia pacifica strain YSBP01 plasmid unnamed1, complete sequence) position: , mismatch: 5, identity: 0.844

gcttcgagaccgacgcgaccgtgg-tgcaggcc	CRISPR spacer
gcttcgagaccgacgcgaccctgattgccggg-	Protospacer
******************** **. *** **

16. spacer 4.37|1832821|29|NZ_CP005986|PILER-CR matches to NZ_CP021814 (Sinorhizobium meliloti strain M270 plasmid psymB, complete sequence) position: , mismatch: 5, identity: 0.828

ccgtaccggaccccgcgccgcccacgcta	CRISPR spacer
ccgtatcggaccccgcgccgccgtcgatg	Protospacer
*****.****************  ** *.

17. spacer 3.2|1830838|28|NZ_CP005986|PILER-CR matches to NZ_CP026333 (Pseudomonas sp. XWY-1 plasmid pXWY, complete sequence) position: , mismatch: 6, identity: 0.786

gatgctgaaaagttggggcaggtctggt	CRISPR spacer
attagtgaaaaggtggggcaggtcaggt	Protospacer
. *. ******* *********** ***

18. spacer 3.2|1830838|28|NZ_CP005986|PILER-CR matches to MN310372 (Pseudomonas putida strain 150716811 plasmid p716811-VIM, complete sequence) position: , mismatch: 6, identity: 0.786

gatgctgaaaagttggggcaggtctggt	CRISPR spacer
attagtgaaaaggtggggcaggtcaggt	Protospacer
. *. ******* *********** ***

19. spacer 3.2|1830838|28|NZ_CP005986|PILER-CR matches to NC_022739 (Pseudomonas sp. VLB120 plasmid pSTY, complete sequence) position: , mismatch: 6, identity: 0.786

gatgctgaaaagttggggcaggtctggt	CRISPR spacer
attagtgaaaaggtggggcaggtcaggt	Protospacer
. *. ******* *********** ***

20. spacer 4.13|1832818|32|NZ_CP005986|CRISPRCasFinder matches to NZ_CP040720 (Rhodococcus pyridinivorans strain YF3 plasmid unnamed1, complete sequence) position: , mismatch: 6, identity: 0.812

caaccgtaccggaccccgcgccgcccacgcta-	CRISPR spacer
agaccggaccggaccccgggccgccga-gctac	Protospacer
 .**** *********** ****** * ****

21. spacer 4.18|1832151|36|NZ_CP005986|CRT matches to NC_009471 (Acidiphilium cryptum JF-5 plasmid pACRY05, complete sequence) position: , mismatch: 6, identity: 0.833

actggcttcgagaccgacgcgaccgtggtgcaggcc	CRISPR spacer
gaaggcttcgagaccgtcgcgagcgtggtgcaggca	Protospacer
.  ************* ***** ************

22. spacer 4.18|1832151|36|NZ_CP005986|CRT matches to NC_015187 (Acidiphilium multivorum AIU301 plasmid pACMV2, complete sequence) position: , mismatch: 6, identity: 0.833

actggcttcgagaccgacgcgaccgtggtgcaggcc	CRISPR spacer
gaaggcttcgagaccgtcgcgagcgtggtgcaggca	Protospacer
.  ************* ***** ************

23. spacer 4.37|1832821|29|NZ_CP005986|PILER-CR matches to NZ_LR594673 (Variovorax sp. PBL-E5 plasmid 3) position: , mismatch: 6, identity: 0.793

ccgtaccggaccccgcgccgcccacgcta	CRISPR spacer
cagcaccggacccagcgcctcccacgcct	Protospacer
* *.********* ***** *******.

24. spacer 4.13|1832818|32|NZ_CP005986|CRISPRCasFinder matches to KY092483 (Streptomyces phage Bioscum, complete genome) position: , mismatch: 7, identity: 0.781

-caaccgtaccggaccccgcgccgcccacgcta	CRISPR spacer
gccgccg-accggtcgccgcgccgcccacgcgc	Protospacer
 * .*** ***** * ***************

25. spacer 4.13|1832818|32|NZ_CP005986|CRISPRCasFinder matches to MK305888 (Streptomyces phage Austintatious, complete genome) position: , mismatch: 7, identity: 0.781

-caaccgtaccggaccccgcgccgcccacgcta	CRISPR spacer
gccgccg-accggtcgccgcgccgcccacgcgc	Protospacer
 * .*** ***** * ***************

26. spacer 4.13|1832818|32|NZ_CP005986|CRISPRCasFinder matches to NC_047792 (Streptomyces phage Ididsumtinwong, complete genome) position: , mismatch: 7, identity: 0.781

-caaccgtaccggaccccgcgccgcccacgcta	CRISPR spacer
gccgccg-accggtcgccgcgccgcccacgcgc	Protospacer
 * .*** ***** * ***************

27. spacer 4.13|1832818|32|NZ_CP005986|CRISPRCasFinder matches to KY092481 (Streptomyces phage PapayaSalad, complete genome) position: , mismatch: 7, identity: 0.781

-caaccgtaccggaccccgcgccgcccacgcta	CRISPR spacer
gccgccg-accggtcgccgcgccgcccacgcgc	Protospacer
 * .*** ***** * ***************

28. spacer 4.37|1832821|29|NZ_CP005986|PILER-CR matches to NZ_CP040820 (Paraoceanicella profunda strain D4M1 plasmid pD4M1B, complete sequence) position: , mismatch: 7, identity: 0.759

ccgtaccggaccccgcgccgcccacgcta	CRISPR spacer
ccgcaccggacccggcgccgcccgccgcc	Protospacer
***.********* *********.*  .

29. spacer 4.37|1832821|29|NZ_CP005986|PILER-CR matches to KY092483 (Streptomyces phage Bioscum, complete genome) position: , mismatch: 7, identity: 0.759

ccgtaccggaccccgcgccgcccacgcta	CRISPR spacer
gccgaccggtcgccgcgccgcccacgcgc	Protospacer
 *  ***** * ***************

30. spacer 4.37|1832821|29|NZ_CP005986|PILER-CR matches to MK305888 (Streptomyces phage Austintatious, complete genome) position: , mismatch: 7, identity: 0.759

ccgtaccggaccccgcgccgcccacgcta	CRISPR spacer
gccgaccggtcgccgcgccgcccacgcgc	Protospacer
 *  ***** * ***************

31. spacer 4.37|1832821|29|NZ_CP005986|PILER-CR matches to NC_047792 (Streptomyces phage Ididsumtinwong, complete genome) position: , mismatch: 7, identity: 0.759

ccgtaccggaccccgcgccgcccacgcta	CRISPR spacer
gccgaccggtcgccgcgccgcccacgcgc	Protospacer
 *  ***** * ***************

32. spacer 4.37|1832821|29|NZ_CP005986|PILER-CR matches to KY092481 (Streptomyces phage PapayaSalad, complete genome) position: , mismatch: 7, identity: 0.759

ccgtaccggaccccgcgccgcccacgcta	CRISPR spacer
gccgaccggtcgccgcgccgcccacgcgc	Protospacer
 *  ***** * ***************

33. spacer 4.1|1832094|32|NZ_CP005986|CRISPRCasFinder matches to NZ_CP039697 (Novosphingobium sp. ABRDHK2 plasmid pABRDHK22, complete sequence) position: , mismatch: 8, identity: 0.75

tgggcatctcccaatcgcgcgcgcaggtgtgc	CRISPR spacer
ggtacagctccgaatcgcgcgcgcaggggcgg	Protospacer
 * .** **** *************** *.*

34. spacer 4.2|1832155|32|NZ_CP005986|CRISPRCasFinder matches to NZ_CP030074 (Streptomyces sp. ZFG47 plasmid unnamed1, complete sequence) position: , mismatch: 8, identity: 0.75

gcttcgagaccgacgcgaccgtggtgcaggcc	CRISPR spacer
cctccgagaccgacgcgcccgtggggaccgtc	Protospacer
 **.************* ****** *   *.*

35. spacer 4.37|1832821|29|NZ_CP005986|PILER-CR matches to NZ_CP049029 (Fluviibacterium aquatile strain SC52 plasmid pSC52_1, complete sequence) position: , mismatch: 8, identity: 0.724

ccgtaccggaccccgcgccgcccacgcta	CRISPR spacer
ccgtacctgaccccgcgccgcatccatgg	Protospacer
******* ************* . *.. .

36. spacer 4.2|1832155|32|NZ_CP005986|CRISPRCasFinder matches to NZ_CP044425 (Paracoccus pantotrophus strain DSM 2944 plasmid pPAN2, complete sequence) position: , mismatch: 9, identity: 0.719

gcttcgagaccgacgcgaccgtggtgcaggcc	CRISPR spacer
ccatcgaggccgacgcgaccgcggtgagcatc	Protospacer
 * *****.************.**** . ..*

37. spacer 4.2|1832155|32|NZ_CP005986|CRISPRCasFinder matches to AM419438 (Archaeal BJ1 virus complete genome) position: , mismatch: 9, identity: 0.719

gcttcgagaccgacgcgaccgtggtgcaggcc	CRISPR spacer
gcttcgcgaccggcgcgaccgtgctctacatg	Protospacer
****** *****.********** * .* ..

38. spacer 4.2|1832155|32|NZ_CP005986|CRISPRCasFinder matches to MN855787 (Bacteriophage sp. isolate 71, complete genome) position: , mismatch: 9, identity: 0.719

gcttcgagaccgacgcgaccgtggtgcaggcc	CRISPR spacer
acttcgagatcgacgcgagcgtgggcggcgac	Protospacer
.********.******** *****   . * *

39. spacer 4.2|1832155|32|NZ_CP005986|CRISPRCasFinder matches to NC_048178 (Escherichia phage Sortsne, complete genome) position: , mismatch: 9, identity: 0.719

gcttcgagaccgacgcgaccgtggtgcaggcc	CRISPR spacer
acttcgagatcgacgcgagcgtgggcggcgac	Protospacer
.********.******** *****   . * *

40. spacer 4.2|1832155|32|NZ_CP005986|CRISPRCasFinder matches to NC_008695 (Archaeal BJ1 virus, complete genome) position: , mismatch: 9, identity: 0.719

gcttcgagaccgacgcgaccgtggtgcaggcc	CRISPR spacer
gcttcgcgaccggcgcgaccgtgctctacatg	Protospacer
****** *****.********** * .* ..

41. spacer 4.37|1832821|29|NZ_CP005986|PILER-CR matches to AB920995 (Ralstonia phage RSJ2 DNA, complete genome) position: , mismatch: 9, identity: 0.69

ccgtaccggaccccgcgccgcccacgcta	CRISPR spacer
taaagccggacgccgcgccgcccacgtag	Protospacer
. . .****** **************. .

42. spacer 3.4|1830827|32|NZ_CP005986|CRISPRCasFinder,CRT matches to NZ_CP026333 (Pseudomonas sp. XWY-1 plasmid pXWY, complete sequence) position: , mismatch: 10, identity: 0.688

aatgatgctgaaaagttggggcaggtctggtc	CRISPR spacer
ttcattagtgaaaaggtggggcaggtcaggtt	Protospacer
  .. *. ******* *********** ***.

43. spacer 3.4|1830827|32|NZ_CP005986|CRISPRCasFinder,CRT matches to MN310372 (Pseudomonas putida strain 150716811 plasmid p716811-VIM, complete sequence) position: , mismatch: 10, identity: 0.688

aatgatgctgaaaagttggggcaggtctggtc	CRISPR spacer
ttcattagtgaaaaggtggggcaggtcaggtt	Protospacer
  .. *. ******* *********** ***.

44. spacer 3.4|1830827|32|NZ_CP005986|CRISPRCasFinder,CRT matches to NC_022739 (Pseudomonas sp. VLB120 plasmid pSTY, complete sequence) position: , mismatch: 10, identity: 0.688

aatgatgctgaaaagttggggcaggtctggtc	CRISPR spacer
ttcattagtgaaaaggtggggcaggtcaggtt	Protospacer
  .. *. ******* *********** ***.

45. spacer 4.13|1832818|32|NZ_CP005986|CRISPRCasFinder matches to NZ_CP040820 (Paraoceanicella profunda strain D4M1 plasmid pD4M1B, complete sequence) position: , mismatch: 10, identity: 0.688

caaccgtaccggaccccgcgccgcccacgcta	CRISPR spacer
agcccgcaccggacccggcgccgcccgccgcc	Protospacer
 . ***.********* *********.*  .

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

90682 : 182143

Acidithiobacillus_phage(93.06%)

transposase,terminase,tail,capsid,portal,plate,head,integrase

attL 124588:124604|attR 174050:174066

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_141738424.1\|90682_91615_+	ATP-binding cassette domain-containing protein	A0A2H4PQG7	Staphylococcus_phage	3.1e-22	30.6
WP_004869835.1\|91611_92391_+	ABC transporter permease	NA	NA	NA	NA
WP_004869837.1\|93163_93637_-	hypothetical protein	K4I1C7	Acidithiobacillus_phage	6.6e-85	100.0
WP_004869841.1\|93633_94017_-	hypothetical protein	K4HZX2	Acidithiobacillus_phage	5.0e-67	100.0
WP_004869844.1\|94013_95393_-	recombinase family protein	K4ICM1	Acidithiobacillus_phage	2.4e-260	100.0
WP_004869845.1\|95392_95836_-	DUF2924 domain-containing protein	K4I3X1	Acidithiobacillus_phage	4.9e-82	100.0
WP_004869848.1\|95835_96027_-	hypothetical protein	K4HZ94	Acidithiobacillus_phage	5.2e-25	100.0
WP_004869851.1\|96131_98387_-	hypothetical protein	K4I1D0	Acidithiobacillus_phage	0.0e+00	100.0
WP_004869854.1\|98412_98985_-	PIN domain-containing protein	K4HZX4	Acidithiobacillus_phage	2.8e-106	100.0
WP_004869856.1\|98989_99451_-	helix-turn-helix domain-containing protein	K4ICM4	Acidithiobacillus_phage	3.4e-78	100.0
WP_004869858.1\|99677_99941_+	DNA-binding protein	K4I3X3	Acidithiobacillus_phage	2.4e-44	100.0
WP_004869861.1\|99951_100431_+	hypothetical protein	K4HZ96	Acidithiobacillus_phage	9.0e-82	100.0
WP_004869863.1\|100434_101208_+	hypothetical protein	K4I1D2	Acidithiobacillus_phage	1.9e-142	100.0
WP_153801791.1\|101204_102038_+	AAA family ATPase	K4HZX9	Acidithiobacillus_phage	9.3e-159	100.0
WP_004869867.1\|102041_102668_+	hypothetical protein	K4ICM8	Acidithiobacillus_phage	4.7e-115	100.0
WP_038471353.1\|102731_103253_+	hypothetical protein	K4I3X7	Acidithiobacillus_phage	3.9e-99	100.0
WP_004869870.1\|103249_103996_+	hypothetical protein	K4HZA0	Acidithiobacillus_phage	9.2e-142	100.0
WP_038471357.1\|103992_104247_+	hypothetical protein	K4I1D6	Acidithiobacillus_phage	6.9e-41	100.0
WP_004869874.1\|104243_106544_+	hypothetical protein	K4HZY1	Acidithiobacillus_phage	0.0e+00	100.0
WP_004869876.1\|106684_107152_+	hypothetical protein	K4ICN3	Acidithiobacillus_phage	1.2e-86	100.0
WP_004869878.1\|107148_107406_+	hypothetical protein	K4I3Y0	Acidithiobacillus_phage	2.6e-43	100.0
WP_004869881.1\|107402_107930_+	hypothetical protein	K4HZA2	Acidithiobacillus_phage	3.9e-94	100.0
WP_038471360.1\|108058_108262_+	hypothetical protein	K4I1D9	Acidithiobacillus_phage	3.5e-27	100.0
WP_004869883.1\|108248_108818_+	hypothetical protein	K4HZY5	Acidithiobacillus_phage	1.0e-100	100.0
WP_004869886.1\|109111_109645_+	hypothetical protein	K4ICN8	Acidithiobacillus_phage	1.9e-88	100.0
WP_004869887.1\|109641_111174_+	site-specific DNA-methyltransferase	K4I3Y2	Acidithiobacillus_phage	2.1e-305	100.0
WP_004869889.1\|111170_112457_+	site-specific DNA-methyltransferase	K4HZA5	Acidithiobacillus_phage	8.4e-252	100.0
WP_004869891.1\|112453_112690_+	hypothetical protein	K4I1E3	Acidithiobacillus_phage	5.8e-34	100.0
WP_153801836.1\|112665_112863_-	hypothetical protein	K4HZY7	Acidithiobacillus_phage	2.8e-29	98.5
WP_004869896.1\|112882_113248_-	hypothetical protein	K4ICP1	Acidithiobacillus_phage	1.1e-66	100.0
WP_051620694.1\|113257_113638_-	hypothetical protein	K4I3Y6	Acidithiobacillus_phage	1.4e-69	100.0
WP_004869900.1\|113641_113926_-	hypothetical protein	K4HZA7	Acidithiobacillus_phage	1.4e-42	100.0
WP_004869902.1\|113993_114191_-	hypothetical protein	K4I1E7	Acidithiobacillus_phage	1.2e-29	100.0
WP_004869903.1\|114187_114784_-	DUF3489 domain-containing protein	K4HZZ2	Acidithiobacillus_phage	1.2e-104	100.0
WP_143969850.1\|114817_115060_-	hypothetical protein	A0A1X9I6B9	Streptococcus_phage	1.1e-06	55.1
WP_082179285.1\|115257_117024_+\|terminase	phage terminase large subunit family protein	K4I3Y9	Acidithiobacillus_phage	0.0e+00	99.8
WP_004869909.1\|117020_117233_+	hypothetical protein	K4HZB1	Acidithiobacillus_phage	1.6e-30	100.0
WP_153801837.1\|117292_118687_+\|portal	phage portal protein	K4I1F2	Acidithiobacillus_phage	2.8e-269	100.0
WP_004869915.1\|118689_119952_+	S49 family peptidase	K4HZZ6	Acidithiobacillus_phage	6.6e-209	100.0
WP_004869916.1\|119948_120338_+\|head	head decoration protein	K4ICP8	Acidithiobacillus_phage	8.4e-62	100.0
WP_004869918.1\|120351_121380_+\|capsid	major capsid protein	K4I3Z3	Acidithiobacillus_phage	1.2e-197	100.0
WP_153801792.1\|121373_121709_+	hypothetical protein	K4HZB3	Acidithiobacillus_phage	7.4e-59	100.0
WP_038471373.1\|121705_122257_+	hypothetical protein	K4I1F4	Acidithiobacillus_phage	2.1e-98	100.0
WP_004869923.1\|122253_122730_+\|plate	phage baseplate assembly protein V	K4HZZ9	Acidithiobacillus_phage	2.0e-89	100.0
WP_004869926.1\|122741_123038_+	PAAR domain-containing protein	K4ICQ1	Acidithiobacillus_phage	4.4e-55	100.0
WP_038471377.1\|123034_123394_+	GPW/gp25 family protein	K4I3Z7	Acidithiobacillus_phage	3.8e-61	100.0
WP_004869930.1\|123390_124245_+\|plate	baseplate J/gp47 family protein	K4HZB7	Acidithiobacillus_phage	4.1e-154	100.0
WP_004869932.1\|124282_125854_+\|tail	phage tail protein	K4I1F8	Acidithiobacillus_phage	1.4e-296	100.0
124588:124604	attL	NA	NA	NA	NA
WP_004869934.1\|125887_126580_+	hypothetical protein	K4I002	Acidithiobacillus_phage	2.7e-127	100.0
WP_004869937.1\|126592_129748_+	DUF4815 domain-containing protein	K4ICQ5	Acidithiobacillus_phage	0.0e+00	100.0
WP_004869938.1\|129760_130009_+	hypothetical protein	K4I3Z9	Acidithiobacillus_phage	7.5e-40	100.0
WP_004869939.1\|130023_130551_+	hypothetical protein	K4HZC1	Acidithiobacillus_phage	8.5e-33	100.0
WP_004869941.1\|130572_130884_+	hypothetical protein	K4I1G2	Acidithiobacillus_phage	3.2e-48	100.0
WP_004869942.1\|130883_132296_+	hypothetical protein	K4I004	Acidithiobacillus_phage	1.7e-274	100.0
WP_004869944.1\|132292_132745_+	hypothetical protein	K4ICR0	Acidithiobacillus_phage	7.7e-75	100.0
WP_051620827.1\|132837_133074_+	hypothetical protein	K4I403	Acidithiobacillus_phage	1.3e-38	100.0
WP_004869948.1\|133155_134652_+\|tail	phage tail sheath subtilisin-like domain-containing protein	K4HZC3	Acidithiobacillus_phage	2.1e-286	100.0
WP_004869950.1\|134663_135176_+\|tail	phage major tail tube protein	K4I1G5	Acidithiobacillus_phage	1.0e-91	100.0
WP_004869952.1\|135188_135437_+\|tail	phage tail assembly protein	K4I007	Acidithiobacillus_phage	2.8e-39	100.0
WP_004869954.1\|135433_135580_+	hypothetical protein	NA	NA	NA	NA
WP_004869957.1\|135589_138670_+\|tail	phage tail tape measure protein	K4ICR4	Acidithiobacillus_phage	0.0e+00	100.0
WP_004869960.1\|138700_139570_+\|tail	phage tail protein	K4I406	Acidithiobacillus_phage	7.7e-156	100.0
WP_038472423.1\|139587_139800_+	hypothetical protein	K4I1H0	Acidithiobacillus_phage	9.9e-33	98.6
WP_004869964.1\|139803_140871_+\|tail	phage-like tail protein	K4HZC6	Acidithiobacillus_phage	3.4e-198	100.0
WP_004869968.1\|141138_141615_+	hypothetical protein	K4ICR8	Acidithiobacillus_phage	4.3e-84	100.0
WP_004869971.1\|141611_142094_+	lysozyme	K4I410	Acidithiobacillus_phage	2.6e-89	99.4
WP_004869972.1\|142422_143772_+	DNA cytosine methyltransferase	K4HZD0	Acidithiobacillus_phage	1.3e-258	100.0
WP_004869975.1\|143832_145815_+	AAA family ATPase	K4I1H4	Acidithiobacillus_phage	0.0e+00	100.0
WP_004869977.1\|145792_147118_+	hypothetical protein	K4I013	Acidithiobacillus_phage	1.5e-251	100.0
WP_004869982.1\|149007_150567_+\|transposase	IS21 family transposase	K4I413	Acidithiobacillus_phage	8.5e-299	100.0
WP_004869984.1\|150589_151324_+	ATP-binding protein	K4HZD4	Acidithiobacillus_phage	3.5e-133	100.0
WP_004869988.1\|152630_152789_-	hypothetical protein	NA	NA	NA	NA
WP_014002076.1\|153028_154153_-	Fic family protein	NA	NA	NA	NA
WP_004869991.1\|154965_156420_+	DUF3375 domain-containing protein	NA	NA	NA	NA
WP_004868469.1\|156664_157927_+\|transposase	IS256 family transposase	A0A2P1JQX9	Mycobacterium_phage	1.4e-89	45.3
WP_082179224.1\|157866_158541_+	DUF4194 domain-containing protein	NA	NA	NA	NA
WP_004870006.1\|158533_161926_+	hypothetical protein	NA	NA	NA	NA
WP_004870008.1\|161922_163116_+	hypothetical protein	NA	NA	NA	NA
WP_004870010.1\|163178_163442_+	type II toxin-antitoxin system Phd/YefM family antitoxin	NA	NA	NA	NA
WP_004870013.1\|163453_163795_+	type II toxin-antitoxin system RelE/ParE family toxin	NA	NA	NA	NA
WP_004870015.1\|164354_165662_-	ParB/RepB/Spo0J family partition protein	A0A160DCL1	Gordonia_phage	3.7e-05	35.1
WP_004870017.1\|165654_166434_-	ParA family protein	NA	NA	NA	NA
WP_004870019.1\|166430_166862_-	hypothetical protein	NA	NA	NA	NA
WP_038472437.1\|166954_167500_-	S26 family signal peptidase	NA	NA	NA	NA
WP_014002082.1\|167538_167823_-	hypothetical protein	NA	NA	NA	NA
WP_038471395.1\|167829_168054_-	hypothetical protein	NA	NA	NA	NA
WP_038471398.1\|168050_168326_-	hypothetical protein	NA	NA	NA	NA
WP_004870029.1\|169665_169863_-	hypothetical protein	NA	NA	NA	NA
WP_004870032.1\|169859_170654_-	ATP-binding protein	NA	NA	NA	NA
WP_038471401.1\|170656_171970_-\|integrase,transposase	DDE-type integrase/transposase/recombinase	NA	NA	NA	NA
WP_082179225.1\|172103_172355_-	hypothetical protein	NA	NA	NA	NA
WP_014002084.1\|173227_173548_-	hypothetical protein	NA	NA	NA	NA
WP_038471408.1\|174896_175952_+\|transposase	IS5 family transposase	NA	NA	NA	NA
174050:174066	attR	NA	NA	NA	NA
WP_038472440.1\|176405_176861_-	DsrE family protein	NA	NA	NA	NA
WP_014002136.1\|177051_178107_-\|transposase	IS5 family transposase	NA	NA	NA	NA
WP_004873230.1\|178185_179547_-	hypothetical protein	NA	NA	NA	NA
WP_038471414.1\|179732_180212_+	DUF302 domain-containing protein	NA	NA	NA	NA
WP_141738303.1\|180305_180776_-	DsrE family protein	NA	NA	NA	NA
WP_014003081.1\|180925_182143_+\|transposase	ISL3-like element ISAtc2 family transposase	A0A2D1GQC1	Lysinibacillus_phage	8.3e-07	23.2

DBSCAN-SWA_2

195692 : 235810

Streptococcus_phage(20.0%)

transposase,integrase

attL 198780:198793|attR 238065:238078

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_169737323.1\|195692_196712_+\|transposase	IS5 family transposase	NA	NA	NA	NA
WP_141738312.1\|197371_197857_+\|transposase	transposase	NA	NA	NA	NA
WP_004870068.1\|197997_198954_+	hypothetical protein	NA	NA	NA	NA
198780:198793	attL	NA	NA	NA	NA
WP_004870074.1\|199424_200270_-\|transposase	IS5 family transposase	E5E3P6	Burkholderia_phage	1.3e-64	48.5
WP_004870079.1\|203297_203996_-\|transposase	IS110 family transposase	NA	NA	NA	NA
WP_014002108.1\|204786_204879_+	K(+)-transporting ATPase subunit F	NA	NA	NA	NA
WP_038471437.1\|204875_206636_+	potassium-transporting ATPase subunit KdpA	NA	NA	NA	NA
WP_004870085.1\|206640_208701_+	potassium-transporting ATPase subunit KdpB	E4ZFI9	Streptococcus_phage	1.6e-34	26.8
WP_004870087.1\|208711_209314_+	potassium-transporting ATPase subunit KdpC	NA	NA	NA	NA
WP_038471440.1\|209559_210729_+	porin	NA	NA	NA	NA
WP_004870092.1\|210830_211025_+	hypothetical protein	NA	NA	NA	NA
WP_077272696.1\|211070_211973_+	glutamate 5-kinase	A0A1X9I5D0	Streptococcus_phage	8.5e-33	36.5
WP_014002140.1\|212654_213554_-\|transposase	IS1595 family transposase	NA	NA	NA	NA
WP_004870103.1\|214424_216002_+	potassium-transporting ATPase subunit A	NA	NA	NA	NA
WP_004870104.1\|216046_216793_+	response regulator transcription factor	NA	NA	NA	NA
WP_004870107.1\|217036_217306_+	metal-sensitive transcriptional regulator	NA	NA	NA	NA
WP_004870110.1\|217634_217775_+	hypothetical protein	NA	NA	NA	NA
WP_004868019.1\|217774_218032_+	hypothetical protein	NA	NA	NA	NA
WP_077272803.1\|218095_218281_+	hypothetical protein	NA	NA	NA	NA
WP_004870115.1\|218368_221866_-	DEAD/DEAH box helicase	A0A0N7D8L3	Dasychira_pudibunda_nucleopolyhedrovirus	7.1e-43	30.8
WP_038471449.1\|222275_222557_+	HigA family addiction module antidote protein	A0A2P1MXE5	Escherichia_phage	4.0e-05	38.9
WP_004870120.1\|222676_223684_-	hypothetical protein	NA	NA	NA	NA
WP_004873291.1\|223801_224119_-	hypothetical protein	NA	NA	NA	NA
WP_004870123.1\|224102_225665_-\|transposase	IS66 family transposase	A0A0P0ZEB3	Stx2-converting_phage	2.2e-44	30.2
WP_153801812.1\|225673_226081_-	IS66 family insertion sequence element accessory protein TnpB	S5VXZ8	Leptospira_phage	3.2e-11	43.4
WP_004867952.1\|226056_226353_-	hypothetical protein	NA	NA	NA	NA
WP_051620702.1\|226547_227108_-	cytochrome b/b6 domain-containing protein	NA	NA	NA	NA
WP_077272805.1\|229143_229425_+	DUF4168 domain-containing protein	NA	NA	NA	NA
WP_004870130.1\|229857_230862_-	hypothetical protein	NA	NA	NA	NA
WP_004870132.1\|230922_232197_-	Y-family DNA polymerase	I6RSM4	Salmonella_phage	2.5e-78	40.2
WP_004870134.1\|232193_232661_-	translesion error-prone DNA polymerase V autoproteolytic subunit	A0A1W6JNS2	Morganella_phage	2.8e-27	49.6
WP_038471461.1\|232781_233279_+	LEA type 2 family protein	NA	NA	NA	NA
WP_004870141.1\|233350_233905_+	FUSC family protein	NA	NA	NA	NA
WP_004870143.1\|234637_235810_-\|integrase	tyrosine-type recombinase/integrase	W6MYA3	Pseudomonas_phage	1.0e-49	40.1
238065:238078	attR	NA	NA	NA	NA

DBSCAN-SWA_3

508306 : 574654

Leptospira_phage(18.18%)

transposase,tRNA,protease

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_014002304.1\|508306_509632_-\|protease	ATP-dependent protease ATPase subunit HslU	A0A173GFL6	Erwinia_phage	9.2e-44	29.1
WP_004870635.1\|509634_510177_-\|protease	ATP-dependent protease subunit HslV	NA	NA	NA	NA
WP_004870636.1\|510176_511130_-	tyrosine recombinase XerC	A0A166YH27	Gordonia_phage	1.6e-13	36.2
WP_004870639.1\|511136_511826_-	DUF484 family protein	NA	NA	NA	NA
WP_004870641.1\|511822_512695_-	diaminopimelate epimerase	NA	NA	NA	NA
WP_004870642.1\|512891_513806_+	NAD(P)-dependent oxidoreductase	NA	NA	NA	NA
WP_038471545.1\|513863_514598_+	SDR family oxidoreductase	NA	NA	NA	NA
WP_004870646.1\|514610_514844_-	hypothetical protein	NA	NA	NA	NA
WP_038471548.1\|515314_516550_+	porin	NA	NA	NA	NA
WP_038471551.1\|516820_519598_+	PilZ domain-containing protein	NA	NA	NA	NA
WP_004870661.1\|519676_520783_-	cellulase	NA	NA	NA	NA
WP_004870663.1\|520817_521543_-	cellulose biosynthesis protein BcsS	NA	NA	NA	NA
WP_004870664.1\|521559_524097_-	cellulose synthase	NA	NA	NA	NA
WP_169737308.1\|524093_526349_-	UDP-forming cellulose synthase catalytic subunit	M1I518	Acanthocystis_turfacea_Chlorella_virus	2.9e-21	27.8
WP_004870667.1\|526714_527722_-	sodium:calcium antiporter	NA	NA	NA	NA
WP_004870668.1\|527796_528516_+	hypothetical protein	NA	NA	NA	NA
WP_038471554.1\|528595_530434_+	DEAD/DEAH box helicase	Q5GF26	Diachasmimorpha_longicaudata_entomopoxvirus	1.0e-56	35.3
WP_014002322.1\|530593_530797_-	cold-shock protein	A0A2H4N7Y6	Lake_Baikal_phage	2.1e-16	60.6
WP_038471557.1\|531130_532198_+	transporter	NA	NA	NA	NA
WP_004867981.1\|532279_533731_-	hypothetical protein	NA	NA	NA	NA
WP_051620728.1\|533727_535080_-	glycosyltransferase	NA	NA	NA	NA
WP_141738584.1\|535271_535964_-	hypothetical protein	NA	NA	NA	NA
WP_004867971.1\|536051_536762_-	C39 family peptidase	NA	NA	NA	NA
WP_004867968.1\|536827_538159_-	hypothetical protein	NA	NA	NA	NA
WP_153801796.1\|538212_538359_-	hypothetical protein	NA	NA	NA	NA
WP_014002140.1\|538364_539264_-\|transposase	IS1595 family transposase	NA	NA	NA	NA
WP_004867966.1\|539287_539824_-	hypothetical protein	NA	NA	NA	NA
WP_004867963.1\|539854_540163_-	hypothetical protein	NA	NA	NA	NA
WP_004867962.1\|540467_541868_-	sigma-54-dependent Fis family transcriptional regulator	NA	NA	NA	NA
WP_153801842.1\|541961_544136_-	primosomal protein N'	NA	NA	NA	NA
WP_004870671.1\|544314_545175_-	4-hydroxybenzoate octaprenyltransferase	NA	NA	NA	NA
WP_038471562.1\|545177_545696_-	chorismate lyase	NA	NA	NA	NA
WP_004870673.1\|545924_546818_+	cation transporter	A0A1V0SED0	Indivirus	1.9e-08	26.2
WP_004870674.1\|546798_547380_-	methyltransferase domain-containing protein	NA	NA	NA	NA
WP_004870675.1\|547610_548921_+	glucose-1-phosphate adenylyltransferase	NA	NA	NA	NA
WP_004870676.1\|548924_550631_+	glycoside hydrolase	NA	NA	NA	NA
WP_004870677.1\|550631_552641_+	DUF1926 domain-containing protein	NA	NA	NA	NA
WP_004870678.1\|552630_553578_+	ROK family protein	NA	NA	NA	NA
WP_004870679.1\|553686_554511_+	glutamate racemase	NA	NA	NA	NA
WP_004870680.1\|554497_554824_-	hypothetical protein	NA	NA	NA	NA
WP_004870681.1\|555022_556312_-	DUF445 domain-containing protein	NA	NA	NA	NA
WP_004870682.1\|556625_558137_+	YdiU family protein	NA	NA	NA	NA
WP_077272691.1\|558218_559568_+\|tRNA	tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB	NA	NA	NA	NA
WP_038471570.1\|559567_560560_+	PhoH family protein	A0A0S0MVD6	Pseudomonas_phage	1.6e-45	45.5
WP_004870685.1\|560556_561066_+	rRNA maturation RNase YbeY	NA	NA	NA	NA
WP_004870686.1\|561058_561937_+	CBS domain-containing protein	NA	NA	NA	NA
WP_004870687.1\|561983_563510_+	apolipoprotein N-acyltransferase	NA	NA	NA	NA
WP_004870688.1\|563893_564982_+	hypothetical protein	NA	NA	NA	NA
WP_004870689.1\|565104_566160_+\|transposase	IS5 family transposase	NA	NA	NA	NA
WP_153801797.1\|566159_566309_+	hypothetical protein	NA	NA	NA	NA
WP_038471573.1\|566649_567591_+	glycosyltransferase family 2 protein	U5P087	Shigella_phage	1.1e-83	52.2
WP_141738408.1\|567581_569321_+	hypothetical protein	NA	NA	NA	NA
WP_004870692.1\|569674_569995_+	hypothetical protein	NA	NA	NA	NA
WP_004870693.1\|569996_570362_+	IS66 family insertion sequence element accessory protein TnpB	S5VXZ8	Leptospira_phage	6.3e-11	31.1
WP_004870694.1\|570377_570824_+	hypothetical protein	NA	NA	NA	NA
WP_014002938.1\|570859_571177_-	hypothetical protein	NA	NA	NA	NA
WP_038471577.1\|571160_572723_-\|transposase	IS66 family transposase	A0A0P0ZEB3	Stx2-converting_phage	2.9e-44	30.0
WP_148262189.1\|572731_573139_-	IS66 family insertion sequence element accessory protein TnpB	S5VXZ8	Leptospira_phage	4.9e-12	35.4
WP_004867952.1\|573114_573411_-	hypothetical protein	NA	NA	NA	NA
WP_004867950.1\|573445_574654_+\|transposase	IS66 family transposase	NA	NA	NA	NA

DBSCAN-SWA_4

591500 : 604037

Staphylococcus_phage(37.5%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_004870712.1\|591500_593006_-	Do family serine endopeptidase	A0A1B1IT49	uncultured_Mediterranean_phage	4.0e-11	23.1
WP_004870713.1\|593192_594062_-	thioredoxin	A0A1J0GW78	Streptomyces_phage	5.0e-14	42.4
WP_004870714.1\|594170_594725_-	hypothetical protein	NA	NA	NA	NA
WP_004870716.1\|595767_597012_+	serine hydroxymethyltransferase	A0A219Y950	Aeromonas_phage	1.7e-95	53.5
WP_004870717.1\|596984_597506_+	transcriptional repressor NrdR	NA	NA	NA	NA
WP_004870718.1\|597516_598626_+	bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD	A0A2H4PQS8	Staphylococcus_phage	2.5e-42	33.6
WP_038471582.1\|598598_599255_+	riboflavin synthase	A0A1V0SE20	Indivirus	3.1e-16	34.6
WP_004870720.1\|599251_600385_+	3,4-dihydroxy-2-butanone-4-phosphate synthase	A0A2H4PQS2	Staphylococcus_phage	1.9e-53	35.7
WP_004870721.1\|600377_600845_+	6,7-dimethyl-8-ribityllumazine synthase	A0A2H4PQS3	Staphylococcus_phage	1.2e-33	53.6
WP_004870722.1\|600869_601313_+	transcription antitermination factor NusB	NA	NA	NA	NA
WP_103415105.1\|601420_601984_+	sel1 repeat family protein	NA	NA	NA	NA
WP_004867332.1\|602004_602337_-	LapA family protein	NA	NA	NA	NA
WP_014002389.1\|602351_604037_-	2-polyprenylphenol 6-hydroxylase	A0A2N9QVT9	Dishui_lake_phycodnavirus	3.8e-34	27.9

DBSCAN-SWA_5

640326 : 662239

Mycobacterium_phage(40.0%)

transposase,integrase

attL 642560:642574|attR 666629:666643

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_038471602.1\|640326_641604_+\|integrase	site-specific integrase	NA	NA	NA	NA
WP_004870689.1\|641890_642946_+\|transposase	IS5 family transposase	NA	NA	NA	NA
642560:642574	attL	NA	NA	NA	NA
WP_153801797.1\|642945_643095_+	hypothetical protein	NA	NA	NA	NA
WP_153801798.1\|643280_644402_+\|integrase	site-specific integrase	NA	NA	NA	NA
WP_004870797.1\|644394_645567_+	hypothetical protein	NA	NA	NA	NA
WP_004868469.1\|645611_646874_+\|transposase	IS256 family transposase	A0A2P1JQX9	Mycobacterium_phage	1.4e-89	45.3
WP_051620734.1\|646947_647865_+	hypothetical protein	NA	NA	NA	NA
WP_169737309.1\|648638_651356_+	ATP-dependent helicase	S5M596	Bacillus_phage	2.1e-34	24.4
WP_004870807.1\|651472_654385_+	DNA methylase	G8I4P9	Mycobacterium_phage	4.1e-20	24.2
WP_004870808.1\|654391_657535_+	DEAD/DEAH box helicase family protein	NA	NA	NA	NA
WP_014002938.1\|658957_659275_-	hypothetical protein	NA	NA	NA	NA
WP_014002963.1\|659258_660821_-\|transposase	IS66 family transposase	A0A0P0ZEB3	Stx2-converting_phage	1.3e-44	30.3
WP_148262189.1\|660829_661237_-	IS66 family insertion sequence element accessory protein TnpB	S5VXZ8	Leptospira_phage	4.9e-12	35.4
WP_004867952.1\|661212_661509_-	hypothetical protein	NA	NA	NA	NA
WP_004870811.1\|662005_662239_-\|integrase	phage integrase N-terminal SAM-like domain-containing protein	NA	NA	NA	NA
666629:666643	attR	NA	NA	NA	NA

DBSCAN-SWA_6

1328179 : 1362919

Burkholderia_phage(40.0%)

transposase

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_169737325.1\|1328179_1329124_+\|transposase	IS5 family transposase	E5E3P6	Burkholderia_phage	3.2e-75	49.7
WP_153801806.1\|1329361_1330369_-	GTP-binding protein	NA	NA	NA	NA
WP_004872068.1\|1330644_1332270_-	GGDEF domain-containing protein	NA	NA	NA	NA
WP_070122140.1\|1332317_1333199_-	GGDEF domain-containing protein	NA	NA	NA	NA
WP_038471791.1\|1333334_1333754_+	hypothetical protein	NA	NA	NA	NA
WP_004872071.1\|1333870_1334371_+	flagellar basal body-associated FliL family protein	NA	NA	NA	NA
WP_004872078.1\|1335240_1336164_+	flagellar motor protein MotB	NA	NA	NA	NA
WP_153801807.1\|1336227_1337235_+	GGDEF domain-containing protein	NA	NA	NA	NA
WP_004868469.1\|1337241_1338504_-\|transposase	IS256 family transposase	A0A2P1JQX9	Mycobacterium_phage	1.4e-89	45.3
WP_004873226.1\|1338627_1339689_+	EAL domain-containing protein	NA	NA	NA	NA
WP_004873225.1\|1339754_1341068_+	diguanylate cyclase	NA	NA	NA	NA
WP_038472710.1\|1341096_1341795_+	hypothetical protein	NA	NA	NA	NA
WP_038472712.1\|1341928_1342696_+	acyltransferase	NA	NA	NA	NA
WP_004873222.1\|1342734_1344819_+	acyltransferase	NA	NA	NA	NA
WP_014002833.1\|1345658_1346714_+\|transposase	IS5 family transposase	NA	NA	NA	NA
WP_038471793.1\|1346945_1347914_+\|transposase	IS5 family transposase	Q38213	Escherichia_phage	4.8e-74	48.0
WP_169737326.1\|1348096_1349041_+\|transposase	IS5 family transposase	E5E3P6	Burkholderia_phage	1.5e-75	50.3
WP_004872099.1\|1350850_1351582_+	ATP-binding protein	A0A2L1IVB6	Escherichia_phage	3.3e-35	38.6
WP_004868266.1\|1352323_1353616_-	outer membrane porin, OprD family	NA	NA	NA	NA
WP_038471796.1\|1353680_1354856_-	TDT family transporter	NA	NA	NA	NA
WP_004872104.1\|1355814_1356894_-	cytochrome d ubiquinol oxidase subunit II	NA	NA	NA	NA
WP_038471800.1\|1356942_1358397_-	cytochrome ubiquinol oxidase subunit I	NA	NA	NA	NA
WP_014002944.1\|1358672_1359404_+	Crp/Fnr family transcriptional regulator	NA	NA	NA	NA
WP_070118376.1\|1361199_1361538_+	hypothetical protein	NA	NA	NA	NA
WP_038472722.1\|1361899_1362919_+\|transposase	IS5 family transposase	NA	NA	NA	NA

DBSCAN-SWA_7

1574552 : 1611845

Burkholderia_phage(16.67%)

transposase,protease

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_004872415.1\|1574552_1575428_-\|protease	protease modulator HflC	NA	NA	NA	NA
WP_004872416.1\|1575424_1576588_-\|protease	FtsH protease activity modulator HflK	NA	NA	NA	NA
WP_004872417.1\|1576603_1577884_-	GTPase HflX	NA	NA	NA	NA
WP_004872418.1\|1577922_1578189_-	RNA chaperone Hfq	NA	NA	NA	NA
WP_004872419.1\|1578468_1581261_+	phosphoenolpyruvate carboxylase	NA	NA	NA	NA
WP_014003043.1\|1581265_1581835_+	septum formation inhibitor Maf	NA	NA	NA	NA
WP_004872644.1\|1582159_1582642_-	tryptophan-rich sensory protein	NA	NA	NA	NA
WP_004872494.1\|1582928_1583342_-	hypothetical protein	NA	NA	NA	NA
WP_169737325.1\|1583475_1584420_-\|transposase	IS5 family transposase	E5E3P6	Burkholderia_phage	3.2e-75	49.7
WP_038471849.1\|1584643_1585000_-	hypothetical protein	NA	NA	NA	NA
WP_038471851.1\|1585923_1586913_+	hypothetical protein	NA	NA	NA	NA
WP_004872422.1\|1588872_1589541_+	hypothetical protein	NA	NA	NA	NA
WP_038471853.1\|1590183_1590546_+	hypothetical protein	NA	NA	NA	NA
WP_038471855.1\|1590983_1591235_-	hypothetical protein	NA	NA	NA	NA
WP_004872425.1\|1591244_1591565_-	hypothetical protein	NA	NA	NA	NA
WP_038471857.1\|1591880_1592438_-	hypothetical protein	NA	NA	NA	NA
WP_004872427.1\|1592584_1595680_-	hypothetical protein	NA	NA	NA	NA
WP_038471859.1\|1595745_1596144_-	hypothetical protein	NA	NA	NA	NA
WP_004872430.1\|1596211_1596757_-	hypothetical protein	NA	NA	NA	NA
WP_038471861.1\|1596744_1597137_-	hypothetical protein	NA	NA	NA	NA
WP_051620751.1\|1597138_1599247_-	DEAD/DEAH box helicase	A0A2I7RS77	Vibrio_phage	5.4e-30	29.1
WP_004872432.1\|1599243_1601052_-	AAA family ATPase	A0A126DN25	Acinetobacter_phage	1.4e-37	26.6
WP_004872433.1\|1601171_1601933_-	DUF669 domain-containing protein	NA	NA	NA	NA
WP_004872434.1\|1602031_1603108_-	AAA family ATPase	A0A0K2CZH1	Paenibacillus_phage	6.2e-22	28.5
WP_004872435.1\|1603134_1604166_-	hypothetical protein	NA	NA	NA	NA
WP_153801809.1\|1604179_1605961_-	single-stranded-DNA-specific exonuclease RecJ	A7KV88	Bacillus_phage	5.6e-52	29.8
WP_004872438.1\|1606017_1606221_-	hypothetical protein	NA	NA	NA	NA
WP_004872439.1\|1606653_1608045_-\|transposase	transposase	NA	NA	NA	NA
WP_153801847.1\|1608184_1609204_-\|transposase	IS5 family transposase	NA	NA	NA	NA
WP_004868469.1\|1610582_1611845_+\|transposase	IS256 family transposase	A0A2P1JQX9	Mycobacterium_phage	1.4e-89	45.3

DBSCAN-SWA_8

1618970 : 1668315

Stx2-converting_phage(33.33%)

transposase,integrase,protease

attL 1624019:1624032|attR 1669088:1669101

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_051620752.1\|1618970_1619471_+\|protease	hydrogenase maturation protease	NA	NA	NA	NA
WP_051620853.1\|1619901_1620882_+	hypothetical protein	NA	NA	NA	NA
WP_038471870.1\|1621144_1621369_+	hypothetical protein	NA	NA	NA	NA
WP_153801848.1\|1621439_1622306_+	NifU family protein	NA	NA	NA	NA
WP_004872448.1\|1622308_1623505_+	hypothetical protein	NA	NA	NA	NA
WP_004872449.1\|1623501_1625913_+	carbamoyltransferase HypF	NA	NA	NA	NA
1624019:1624032	attL	NA	NA	NA	NA
WP_004872450.1\|1625903_1626197_+	HypC/HybG/HupF family hydrogenase formation chaperone	NA	NA	NA	NA
WP_004872451.1\|1626206_1626890_+	SIS domain-containing protein	NA	NA	NA	NA
WP_004872452.1\|1626892_1628038_+	hydrogenase formation protein HypD	NA	NA	NA	NA
WP_004872453.1\|1628037_1629096_+	hydrogenase expression/formation protein HypE	NA	NA	NA	NA
WP_004872454.1\|1629109_1629439_+	hydrogenase maturation nickel metallochaperone HypA	NA	NA	NA	NA
WP_038471873.1\|1629438_1630335_+	hydrogenase nickel incorporation protein HypB	NA	NA	NA	NA
WP_004872457.1\|1630760_1632185_+	APC family permease	NA	NA	NA	NA
WP_051620753.1\|1632638_1633094_-	restriction endonuclease	NA	NA	NA	NA
WP_153801847.1\|1633510_1634530_+\|transposase	IS5 family transposase	NA	NA	NA	NA
WP_153801810.1\|1634789_1635836_+	hypothetical protein	NA	NA	NA	NA
WP_153801811.1\|1635863_1636496_+	hypothetical protein	NA	NA	NA	NA
WP_038471879.1\|1636662_1638846_-	ATP-dependent RecD-like DNA helicase	A0A1P8DII4	Virus_Rctr197k	8.1e-53	33.6
WP_038471882.1\|1638842_1639238_-	hypothetical protein	NA	NA	NA	NA
WP_004872463.1\|1639343_1639613_+	hypothetical protein	NA	NA	NA	NA
WP_004872464.1\|1640169_1641183_+	fatty acid desaturase	NA	NA	NA	NA
WP_004872465.1\|1641302_1641929_+	TetR/AcrR family transcriptional regulator	NA	NA	NA	NA
WP_004872466.1\|1641970_1642861_-	patatin-like phospholipase family protein	NA	NA	NA	NA
WP_051620855.1\|1643100_1644378_+	MFS transporter	NA	NA	NA	NA
WP_038471885.1\|1644603_1645449_+	hypothetical protein	A0A2I6PCS6	Staphylococcus_phage	5.8e-07	30.4
WP_014002140.1\|1645906_1646806_-\|transposase	IS1595 family transposase	NA	NA	NA	NA
WP_038471888.1\|1647160_1647505_-	P-II family nitrogen regulator	NA	NA	NA	NA
WP_004872118.1\|1647486_1648242_-	type 1 glutamine amidotransferase	NA	NA	NA	NA
WP_082179252.1\|1648279_1648816_-	hypothetical protein	NA	NA	NA	NA
WP_004873291.1\|1649608_1649926_-	hypothetical protein	NA	NA	NA	NA
WP_004870123.1\|1649909_1651472_-\|transposase	IS66 family transposase	A0A0P0ZEB3	Stx2-converting_phage	2.2e-44	30.2
WP_169737312.1\|1651480_1651888_-	IS66 family insertion sequence element accessory protein TnpB	S5VXZ8	Leptospira_phage	8.3e-12	33.9
WP_004867952.1\|1651863_1652160_-	hypothetical protein	NA	NA	NA	NA
WP_169737313.1\|1652297_1652600_-	ATP-binding protein	NA	NA	NA	NA
WP_082179289.1\|1652623_1654114_-	sigma 54-interacting transcriptional regulator	NA	NA	NA	NA
WP_082179254.1\|1654297_1654540_-	zinc ribbon domain-containing protein	NA	NA	NA	NA
WP_038471897.1\|1654600_1655200_-	carbonic anhydrase	NA	NA	NA	NA
WP_004867952.1\|1655670_1655967_+	hypothetical protein	NA	NA	NA	NA
WP_153801812.1\|1655942_1656350_+	IS66 family insertion sequence element accessory protein TnpB	S5VXZ8	Leptospira_phage	3.2e-11	43.4
WP_004870123.1\|1656358_1657921_+\|transposase	IS66 family transposase	A0A0P0ZEB3	Stx2-converting_phage	2.2e-44	30.2
WP_004873291.1\|1657904_1658222_+	hypothetical protein	NA	NA	NA	NA
WP_082179290.1\|1658308_1658425_+	IS66 family insertion sequence element accessory protein TnpB	NA	NA	NA	NA
WP_004873293.1\|1658651_1659119_-	SDR family NAD(P)-dependent oxidoreductase	NA	NA	NA	NA
WP_038471900.1\|1659159_1660170_-	aldo/keto reductase	NA	NA	NA	NA
WP_038472789.1\|1660518_1661850_-	sigma 54-interacting transcriptional regulator	NA	NA	NA	NA
WP_014002140.1\|1662040_1662940_-\|transposase	IS1595 family transposase	NA	NA	NA	NA
WP_004872470.1\|1663589_1664039_-	DUF2442 domain-containing protein	NA	NA	NA	NA
WP_082179291.1\|1664035_1664272_-	DUF4160 domain-containing protein	NA	NA	NA	NA
WP_038471905.1\|1665136_1665415_+	hypothetical protein	NA	NA	NA	NA
WP_153801813.1\|1665899_1666619_+	hypothetical protein	NA	NA	NA	NA
WP_082179292.1\|1666947_1668315_-\|integrase	site-specific integrase	NA	NA	NA	NA
1669088:1669101	attR	NA	NA	NA	NA