CRISPRimmunity

Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Self targeting spacer number	Target MGE spacer number	Prophage number	Anti-CRISPR protein number
NC_017365	Helicobacter pylori F30, complete genome	1 crisprs	cas3,DEDDh	0	4	0	0
NC_017369	Helicobacter pylori F30 plasmid pHPF30, complete sequence	0 crisprs	NA	0	0	0	0

1. NC_017365

CRISPR-Cas detection and classification

Crispr_ID: NC_017365_1

• CRISPR_ID: NC_017365_1
• CRISPR_location: 1044309-1044665
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 7 spacers

Consensus repeat of NC_017365_1

Consensus_repeat	Method
AAAGAAGAAACGCAAGAN	CRT

spacers of NC_017365_1

>1.1|1044327|24|NC_017365|CRT
CAAGCCAAAGAACAAGAGCCAATC
>1.2|1044369|39|NC_017365|CRT
AATAAGGAGGAAAAACAAGAAAAAACGCAAGAAGAAGTT
>1.3|1044426|24|NC_017365|CRT
CAAGCCAAAGAACAAGAGCCAATC
>1.4|1044468|39|NC_017365|CRT
AATAAGGAGGAAAAACAAGAAAAAACGCAAGAAGAAGTT
>1.5|1044525|24|NC_017365|CRT
CAAGCCAAAGAACAAGAGCCAATC
>1.6|1044567|27|NC_017365|CRT
AATAAGGAGGAAAAACAAGAAAAAACG
>1.7|1044612|36|NC_017365|CRT
GAAACGCAAGAGCAAATCAAAGAACAAGAGCCAATC

CRISPR arrays and Neighbor proteins around NC_017365_1

CRISPR arrays

The CRISPR arrays of NC_017365_1

>merge|NC_017365|1|1044309-1044665|CRT
AAAGAAGAAACGCAAGAGCAAGCCAAAGAACAAGAGCCAATCAAAGAAGAAACGCAAGAAAATAAGGAGGAAAAACAAGAAAAAACGCAAGAAGAAGTTAAAGAAGAAACGCAAGAGCAAGCCAAAGAACAAGAGCCAATCAAAGAAGAAACGCAAGAAAATAAGGAGGAAAAACAAGAAAAAACGCAAGAAGAAGTTAAAGAAGAAACGCAAGAGCAAGCCAAAGAACAAGAGCCAATCAAAGAAGAAACGCAAGAAAATAAGGAGGAAAAACAAGAAAAAACGCAAGAAGAAGTTAAAGAAGAAACGCAAGAGCAAATCAAAGAACAAGAGCCAATCAAAGAAGAAACGCAAGAA

>NC_017365|1|1|1044309-1044665|CRT
AAAGAAGAAACGCAAGAG	CAAGCCAAAGAACAAGAGCCAATC
AAAGAAGAAACGCAAGAA	AATAAGGAGGAAAAACAAGAAAAAACGCAAGAAGAAGTT
AAAGAAGAAACGCAAGAG	CAAGCCAAAGAACAAGAGCCAATC
AAAGAAGAAACGCAAGAA	AATAAGGAGGAAAAACAAGAAAAAACGCAAGAAGAAGTT
AAAGAAGAAACGCAAGAG	CAAGCCAAAGAACAAGAGCCAATC
AAAGAAGAAACGCAAGAA	AATAAGGAGGAAAAACAAGAAAAAACG
CAAGAAGAAGTTAAAGAA	GAAACGCAAGAGCAAATCAAAGAACAAGAGCCAATC
AAAGAAGAAACGCAAGAA

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
NC_017365.1|WP_000932528.1|1042964_1043507_-|nuclease	unknown	unknown	gnl|CDD|184389
NC_017365.1|WP_096001115.1|1039813_1040707_-|CMP-N-acetylneuraminic-acid-synthetase	unknown	unknown	gnl|CDD|226488
NC_017365.1|WP_000934598.1|1047953_1048709_+|HugZ-family-heme-oxygenase	unknown	unknown	gnl|CDD|200360
NC_017365.1|WP_000710028.1|1041377_1042091_-|flagellar-basal-body-L-ring-protein-FlgH	unknown	unknown	gnl|CDD|234700
NC_017365.1|WP_000742660.1|1039274_1039817_-|UDP-4-amino-4,-6-dideoxy-N-acetyl-beta-L-altrosamine-N-acetyltransferase	unknown	unknown	gnl|CDD|274661
NC_017365.1|WP_000508604.1|1046025_1046265_+|twin-arginine-translocase-TatA/TatE-family-subunit	unknown	unknown	gnl|CDD|234822
NC_017365.1|WP_014535226.1|1036383_1037376_-|ketol-acid-reductoisomerase	unknown	unknown	gnl|CDD|235491
NC_017365.1|WP_000551231.1|1045335_1045956_+|guanylate-kinase	unknown	unknown	gnl|CDD|213788
NC_017365.1|WP_001263653.1|1049213_1050359_+|YbfB/YjiJ-family-MFS-transporter	unknown	unknown	gnl|CDD|284247
NC_017365.1|WP_001040361.1|1053658_1054558_-|polysaccharide-deacetylase	unknown	unknown	gnl|CDD|200563
NC_017365.1|WP_000094927.1|1052931_1053624_+|hypothetical-protein	unknown	unknown	unknown
NC_017365.1|WP_000833949.1|1038403_1039342_+|tetraacyldisaccharide-4'-kinase	unknown	unknown	gnl|CDD|224577
NC_017365.1|WP_000337870.1|1051329_1051716_-|hypothetical-protein	unknown	unknown	unknown
NC_017365.1|WP_001168229.1|1037624_1038407_+|NAD+-synthase	unknown	unknown	gnl|CDD|184435
NC_017365.1|WP_001154472.1|1051811_1052921_+|site-specific-DNA-methyltransferase	unknown	unknown	gnl|CDD|334588
NC_017365.1|WP_014535227.1|1042220_1042958_+|outer-membrane-protein	unknown	unknown	gnl|CDD|280099
NC_017365.1|WP_000557126.1|1046267_1047893_+|arginine--tRNA-ligase	unknown	unknown	gnl|CDD|273085
NC_017365.1|WP_001124345.1|1050364_1051330_-|GTP-binding-protein	unknown	unknown	gnl|CDD|223597
NC_017365.1|WP_001019100.1|1035553_1036360_-|septum-site-determining-protein-MinD	unknown	unknown	gnl|CDD|225447
NC_017365.1|WP_001201377.1|1040676_1041360_-|pseudaminic-acid-cytidylyltransferase	unknown	unknown	gnl|CDD|274660

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
NC_017365.1|WP_000932528.1|1042964_1043507_-|nuclease	gnl|CDD|184389	PRK13912, PRK13912, nuclease NucT; Provisional.	2.51276e-92
NC_017365.1|WP_096001115.1|1039813_1040707_-|CMP-N-acetylneuraminic-acid-synthetase	gnl|CDD|226488	COG3980, spsG, Spore coat polysaccharide biosynthesis protein, predicted glycosyltransferase [Cell envelope biogenesis, outer membrane].	8.7259e-105
NC_017365.1|WP_000934598.1|1047953_1048709_+|HugZ-family-heme-oxygenase	gnl|CDD|200360	TIGR04109, heme_ox_HugZ, heme oxygenase, HugZ family. Members of this protein family are HugZ, a class of heme oxygenase that belongs to the PPOX family (pfam01243) and lacks homology to the HmuO family (pfam01126). Characterized members of this family include HP0318 from Helicobacter pylori and CJ1613c from Campylobacter jejuni. This enzyme releases iron during the conversion of heme to biliverdin.	6.76119e-134
NC_017365.1|WP_000710028.1|1041377_1042091_-|flagellar-basal-body-L-ring-protein-FlgH	gnl|CDD|234700	PRK00249, flgH, flagellar basal body L-ring protein FlgH.	9.56681e-86
NC_017365.1|WP_000742660.1|1039274_1039817_-|UDP-4-amino-4,-6-dideoxy-N-acetyl-beta-L-altrosamine-N-acetyltransferase	gnl|CDD|274661	TIGR03585, PseH, UDP-4-amino-4,6-dideoxy-N-acetyl-beta-L-altrosamine N-acetyltransferase. Sequences in this family are members of the pfam00583 (GNAT) superfamily of acetyltransferases and are proposed to perform a N-acetylation step in the process of pseudaminic acid biosynthesis in Campylobacter species. This gene is commonly observed in apparent operons with other genes responsible for the biosynthesis of pseudaminic acid and as a component of flagellar and exopolysaccharide biosynthesis loci. Significantly, many genomes containing other components of this pathway lack this gene, indicating that some other N-acetyl transferases may be incolved and/or the step is optional, resulting in a non-acetylated pseudaminic acid variant sugar.	4.14644e-59
NC_017365.1|WP_000508604.1|1046025_1046265_+|twin-arginine-translocase-TatA/TatE-family-subunit	gnl|CDD|234822	PRK00720, tatA, twin-arginine translocase TatA/TatE family subunit.	1.06209e-19
NC_017365.1|WP_014535226.1|1036383_1037376_-|ketol-acid-reductoisomerase	gnl|CDD|235491	PRK05479, PRK05479, ketol-acid reductoisomerase; Provisional.	7.86029e-178
NC_017365.1|WP_000551231.1|1045335_1045956_+|guanylate-kinase	gnl|CDD|213788	TIGR03263, Guanylate_kinase, guanylate kinase. Members of this family are the enzyme guanylate kinase, also called GMP kinase. This enzyme transfers a phosphate from ATP to GMP, yielding ADP and GDP. [Purines, pyrimidines, nucleosides, and nucleotides, Nucleotide and nucleoside interconversions].	1.06842e-99
NC_017365.1|WP_001263653.1|1049213_1050359_+|YbfB/YjiJ-family-MFS-transporter	gnl|CDD|284247	pfam06779, MFS_4, Uncharacterized MFS-type transporter YbfB. This family represents putative bacterial membrane proteins which may be sugar transporters. Members carry twelve transmembrane regions which are characteristic of members of the major facilitator sugar-transporter superfamily.	2.51039e-21
NC_017365.1|WP_001040361.1|1053658_1054558_-|polysaccharide-deacetylase	gnl|CDD|200563	cd10938, CE4_HpPgdA_like, Catalytic domain of Helicobacter pylori peptidoglycan deacetylase (HpPgdA) and similar proteins. This family is represented by a peptidoglycan deacetylase (HP0310, HpPgdA) from the gram-negative pathogen Helicobacter pylori. HpPgdA has the ability to bind a metal ion at the active site and is responsible for a peptidoglycan modification that counteracts the host immune response. It functions as a homotetramer. The monomer is composed of a 7-stranded barrel with detectable sequence similarity to the 6-stranded barrel NodB homology domain of polysaccharide deacetylase (DCA)-like proteins in the CE4 superfamily, which removes N-linked or O-linked acetyl groups from cell wall polysaccharides. In contrast to typical NodB-like DCAs, HpPgdA does not exhibit a solvent-accessible polysaccharide binding groove, suggesting that the enzyme binds a small molecule at the active site.	7.43922e-139
NC_017365.1|WP_000833949.1|1038403_1039342_+|tetraacyldisaccharide-4'-kinase	gnl|CDD|224577	COG1663, LpxK, Tetraacyldisaccharide-1-P 4'-kinase [Cell envelope biogenesis, outer membrane].	1.78002e-88
NC_017365.1|WP_001168229.1|1037624_1038407_+|NAD+-synthase	gnl|CDD|184435	PRK13980, PRK13980, NAD synthetase; Provisional.	8.54077e-125
NC_017365.1|WP_001154472.1|1051811_1052921_+|site-specific-DNA-methyltransferase	gnl|CDD|334588	pfam01555, N6_N4_Mtase, DNA methylase. Members of this family are DNA methylases. The family contains both N-4 cytosine-specific DNA methylases and N-6 Adenine-specific DNA methylases.	9.31161e-42
NC_017365.1|WP_014535227.1|1042220_1042958_+|outer-membrane-protein	gnl|CDD|280099	pfam01856, HP_OMP, Helicobacter outer membrane protein. This family seems confined to Helicobacter. It is predicted to be an outer membrane protein based on its pattern of alternating hydrophobic amino acids similar to porins.	2.10843e-52
NC_017365.1|WP_000557126.1|1046267_1047893_+|arginine--tRNA-ligase	gnl|CDD|273085	TIGR00456, Arginine--tRNA_ligase, arginyl-tRNA synthetase. This model recognizes arginyl-tRNA synthetase in every completed genome to date. An interesting feature of the alignment of all arginyl-tRNA synthetases is a fairly deep split between two families. One family includes archaeal, eukaryotic and organellar, spirochete, E. coli, and Synechocystis sp. The second, sharing a deletion of about 25 residues in the central region relative to the first, includes Bacillus subtilis, Aquifex aeolicus, the Mycoplasmas and Mycobacteria, and the Gram-negative bacterium Helicobacter pylori. [Protein synthesis, tRNA aminoacylation].	0
NC_017365.1|WP_001124345.1|1050364_1051330_-|GTP-binding-protein	gnl|CDD|223597	COG0523, COG0523, Putative GTPases (G3E family) [General function prediction only].	4.9334e-89
NC_017365.1|WP_001019100.1|1035553_1036360_-|septum-site-determining-protein-MinD	gnl|CDD|225447	COG2894, MinD, Septum formation inhibitor-activating ATPase [Cell division and chromosome partitioning].	8.44756e-150
NC_017365.1|WP_001201377.1|1040676_1041360_-|pseudaminic-acid-cytidylyltransferase	gnl|CDD|274660	TIGR03584, PseF, pseudaminic acid cytidylyltransferase. The sequences in this family include the pfam02348 (cytidyltransferase) domain and are homologous to the NeuA protein responsible for the transfer of CMP to neuraminic acid. According to, this gene is responsible for the transfer of CMP to the structurally related sugar, pseudaminic acid which is observed as a component of sugar modifications of flagellin in Campylobacter species. This gene is commonly observed in apparent operons with other genes responsible for the biosynthesis of pseudaminic acid and as a component of flagellar and exopolysaccharide biosynthesis loci.	6.82638e-118

Protein sequences

>NC_017365.1|WP_000932528.1|1042964_1043507_-|nuclease
MLNKFKKIVGVSVLVGCLVVLQAKNSLFVLPYEQREALNALISGISNARESVKIAIYSFTHRDIARAIKSVASKGIKVQIIYDYGSNHSNNQSTIGYLDKYPNTKVCLLKGLKAKNGNYHGIMHQKVAIIDDKIVFLGSANWSKNAFENNYEVLLKTDDTETILKAKSYYQKMLGSCVGF
>NC_017365.1|WP_014535227.1|1042220_1042958_+|outer-membrane-protein
MRTLLKMLVGVSLLTHALVAKEESAAPSWTKNLYMGVNYQTGSINLMTNIHEVREITNYQTGYTNIITSVNSVKKLTNMGSNGIGLVMGYKHFFHPDKILGLRYFAFLDWQGYGMRYPKGYYGGNNMITYGVGVDAMWNFFQGSFYQDDISVDIGVFGGIAIAGNSWYIGNKGQELLGITNSRAVDNTSFQFLFNFGFKALFVDEHEFEIGFKFPTINNKYYTTDALKVQMRRVFAFYVGYNYHF
>NC_017365.1|WP_000710028.1|1041377_1042091_-|flagellar-basal-body-L-ring-protein-FlgH
MKKALYLGAVALSVAFSMASANEPKIDFNPPSYVEETPSKEFIPELNKLGSLFGQGERPLFADRRAMKPNDLITIIVSEKASANYSSSKDYKSASGGNSTPPRLTYNGLDERKKQEAQYLDDKNNYNFTKSSNNTNFKGGGSQKKSEDLEIVLSARIIKVLENGNYFIYGNKEVLVDGEKQILKVSGVIRPYDIERNNTIQSKFLADAKIEYTNLGHLSDSNKKKFAADAMETQMPY
>NC_017365.1|WP_001201377.1|1040676_1041360_-|pseudaminic-acid-cytidylyltransferase
MRAIAIVLARSSSKRIKNKNIIDFFNKPMLAYPIETALNSKLFEKVFISSDSMEYVHLAKNYGASFLNLRPKVLADDRATTLEVMAYHMKELELKDEDVACCLYGTSALLQEKHLKNAFEILKGNTDYVFTCSPFSASPYRSFSLENGVQMAFKEHLNTRTQDLKTLYHDAGLFYMGKAQAFKEMRPIFSQNSIALELSPLEVQDIDTLEDLELAKLKYSRLKNACQ
>NC_017365.1|WP_096001115.1|1039813_1040707_-|CMP-N-acetylneuraminic-acid-synthetase
MQPFEKRMPVKILCDCFLTSGLGHVRRCEKILSFIEKLGVKADLYLYKHNNISAFLEGVGNDDFLIIDSYCLNSKDFYLLKEKAKSLMVIEDEEHVKGFYPKNTKILNFTLNALKHYHYLSKDYQYYLGVGFYPVDMRFVYERPINTENKEVLITLGGSEQKTLKEIVKILENKGMHLHIISPYIPKNPPKNTHYYSPLSPLEFSSLMKFCACAISAAGQTLYELALSQTPSLIIPIASNQIIQSQEFESLGIFKQTSLKTLAKDFENLQIQKNQAWAKNLAFGSELEGALREFLEI
>NC_017365.1|WP_000742660.1|1039274_1039817_-|UDP-4-amino-4,-6-dideoxy-N-acetyl-beta-L-altrosamine-N-acetyltransferase
MKKNYSYGNIQAIDFVHLNDEEKLLVLEFRNHPNTALWMYSAFISLKMHLQFIEDLKNSPNHRYFLFKEEGVYLGVGSITKINFFHKHGYLGIYKNPFLKNRGETILKALERIAFEEFQLNSLHLEVMENNFKAIAFYEKNHYELEGRLKGFISKEKEFIDVLLYYKDKKGYNDRSALKF
>NC_017365.1|WP_000833949.1|1038403_1039342_+|tetraacyldisaccharide-4'-kinase
MKSDKPFLERYFYDPTLLQKGLIFALYPFSLIYQGIATLKRKTAKKHDFKIPLISIGNLIAGGSGKTPFILETAPRYQEVAVVSRGYQRDSKGLVVVSVKGNILVSQQTAGDEAYLLALNLKQASVIVSEKRELGVLKALELGSKIVFLDDGFRFNFNQFNLLLKPKVPPYYPFCLPSGLYRESIKNYKEAHLVITEDKDYKRITSISHPTKRMLLVTAIANPSRLDAFLPKEVVKKLYFRDHAPFDLKLLEKEFYQNNATSLLVTSKDLVKLQDCNLPLSVLNLKLEICPKILGQIDRYILSYPCNTKEHL
>NC_017365.1|WP_001168229.1|1037624_1038407_+|NAD+-synthase
MQKDYQKLIAYLCDFLEKEAQKRGFKKVVYGLSGGLDSTVVGVLCQKVFKENAHALLMPSLVSMPESKTDALDLCEKFSIPYTEYSIAPYDAIFSSHFKDASLTRKGNFCARLRMAFLYDYSLKSDSLVIGTSNKSERMLGYGTLFGDLACAINPIGELFKTEVYELACHLNIPKSILNKPPSADLFVGQSDEKDLGYPYSVIDPLLKDIEALFQTKPIHLETLTQLGYDEILVKNIISRIQKNAFKLELPTIAKRFNPK
>NC_017365.1|WP_014535226.1|1036383_1037376_-|ketol-acid-reductoisomerase
MALPVYYDKDIDLGVIQSLQVGIIGYGTQGEAQALNLRDSKVRVRIGLYQGSLSASKVKAEGFEVLEIKELVQQSDLIMALLPDELHKEVLEKEVIPFLKEGQIVGFAHGFSVHFNQVIIPKGVGTILVAPKGPGSALREEYLKNRGLYHLIAIEQESSKNNAKAVALSYAKAMGGGRMGVLETSFKEECESDLFGEQAVLCGGLEAIVRMGFETLIKAGYPEELAYFECVHEVKLVADLLHYKGVEGLRKHISNTAEFGAIKAREPMGNLLEKRMQKILKKIQNGSFAKDFLLEKSLNYPRLNTERKALKETKIEQIGEILRAPFNHKK
>NC_017365.1|WP_001019100.1|1035553_1036360_-|septum-site-determining-protein-MinD
MAIVVTITSGKGGVGKSTTTANLAIGLAESGKKVVAVDFDIGLRNLDMILGLENRIVYDVVDVMEKNCNLSQALITDKKTKNLSFLAASQSKDKNVLDKEKVAILINALRADFDYILIDSPAGIESGFEHAILHADMALVVVTPEVSSLRDSDRVIGIIDAKSNRAKKGMEVHKHLIINRLKPELVANGEMISIEEVLKILCLPLIGIIPEDSHIISATNKGEPVIRADCESAKAYQRVTRRILGEEVEYVEFKAKKGFFSALKGIFS
>NC_017365.1|WP_000551231.1|1045335_1045956_+|guanylate-kinase
MHNDFNLLILSGPSGAGKSTLTKYLQEKIPKTHFSLSTTTRKPREGEVDGLHYNFVSEEEFKQGIEKGQFLEWAIVHNHYYGTSKIPVEKALKEGKIVIFDIDVQGHEILKKHYPNACSVFISTKNQEILKERLLLRGTDSKETIEKRLINAYKEMQCLESFDYLIINEDLEKSKEIILSIAKTLVYRLKAFNFEKICKAWKNESL
>NC_017365.1|WP_000508604.1|1046025_1046265_+|twin-arginine-translocase-TatA/TatE-family-subunit
MGGFTSVWHWVIVLLVIVLLFGAKKIPELAKGLGSGIKNFKKAVKDDEEEAKNEPKTLDAQAAQTKVHETSEIKSKQES
>NC_017365.1|WP_000557126.1|1046267_1047893_+|arginine--tRNA-ligase
MHTLIKGVLEEILEAEAVIEYPKDREHGHYATPIAFNLAKVFKKSPLVIAEELALKISTHEKTQGFFDSVVACKGYINFTLSLDFLERSTQKALELKERFGSQAKSERSQKIFLEFVSANPTGPLHIGHARGAVFGDSLAKIARFLGHEVLREYYVNDMGSQIRLLGLSVWLAYREHVLQESVTYPEVFYKGEYIIEIAKQANNDLEPSLFKENEETIIEVLSSYAKDLMLLEIKDNLDALGIHFDSYASEREVFKHKDAVFERLEKANALYEKDSKIWLKSSLYQDESDRVLIKEDKSYTYLAGDIVYHDEKFKQNYTKYINIWGADHHGYIARVKASLEFLGYDSNKLEVLLAQMVRLLKDNEPYKMSKRAGNFILIKDVIDDVGKDALRFIFLSKRLDTHLEFDVNTLKKQDSSNPIYYIHYANSRIHTMLEKSPFSKEEILQTPLTNLNAEEKYLLFSALSLPKIIESSFEEYGLQKMCEYAKTLASEFHRFYNAGKILNTPKAKELLKICLMVSLSLSNAFKLLGIEIKTKISAKD
>NC_017365.1|WP_000934598.1|1047953_1048709_+|HugZ-family-heme-oxygenase
MLNRIIEHMNAHHVEDMKGLLKKFGQVHHAENVAFKSVDSQGVVIGYNNNQTLRIEFNHEVKDPKDYKNAIIELCQSVEKTHDLKGVEEEVKAFKEGFDSVCLATLHPNGHVVCSYAPLMSDGKQYYIYVSEVAEHFAGLKHNPHNVEVMFLEDESKAKSAILRKRLRYKTNARFIERGAEFDKAFDSFIEKTGGAGGIKTIRTMQDFHLIALDFKEGRFVKGFGQAYDILGDKIAYVGGKCNPHDFAHKK
>NC_017365.1|WP_001263653.1|1049213_1050359_+|YbfB/YjiJ-family-MFS-transporter
MRVFVCFLGVFVSNGLARFGYVVLIPLLILSGSLTPNQSFQLGIAVLMGYVFGSFLIQFLSPLMSLESIAKISFALIALSFLICYFDSIPFFWLWIWRFIAGVASSALMILVAPLSLPYIKENKKALVGGFIFSAVGIGSVFSGFVLPWISSYNIKWAWIFLGGSCLIAFILSLIGLKTRSLRRKSVKKEESAFKIPFHLWLLLVSCALNAIGFLPHTLFWVDYLIRHLNISPTIAGTSWALFGFGATLGSLISGPMAQKLGAKNANIFILILKSIACFLPIFFHQISLLNLSIFIMGAATTANINLFSMMALKIAGAKHFAKASSWVVFSFGIFQALFSYLFTIFLGDLGYLLIFVICGVCLVLSFIVLFPIKMQTAVNK
>NC_017365.1|WP_001124345.1|1050364_1051330_-|GTP-binding-protein
MPKIPITLITGFLGSGKTSFLSEYLNQIDHQGVALIINEIGQAALDQRILSVQYCGEKMLYLNAGCVCCNKRMDLVESLKATLNSYERHGEILKRIIIETTGLANPAPILWTILSDTFLGVHFEIQSVVVCVDALNARTHLTNNEAKEQIVFADSVLLTKTDLQNDSVALIKLKECLQTINPSAEIFDKKNIHYESFFSRKNRVQNFMPRTPKDSHSQGFETLSINFEGAMEWSAFGIWLSLLLHKYGTQILRIKGIIDIGSGFLVSINGVMHIIYPPKHILKDQNGSNLVFIMRHLEREKILNSLKGFKDFLGIKGFETQ
>NC_017365.1|WP_000337870.1|1051329_1051716_-|hypothetical-protein
MCVLCGELIRSFHWTDGTDGTDGSGSYENDENLKGQNTLVSVNKNARERKRARLKRVGLLNQILAFYGLKIDDWQGAKFVLHDKKGQSVIVNDLGDLWDKAQNLAKKEMDALDSHLLAFLNQNTNAIH
>NC_017365.1|WP_001154472.1|1051811_1052921_+|site-specific-DNA-methyltransferase
MQDYRTLTLENIYAPDEITDALSLLQSCGIESIVNPSNITLNFDIVDLKMLESTTQNTLVQKTLEELYKIRSFSSQNGKIVFKSFNKAKKVANKKQNAKARLAYESYKKEFSKETNEIQSCLNQIYCEDSLEFLKKLPNNCIDIVLTSPPYNFGINYSATQDTNLWQEYFNTLFAIFKECIRVLKSGGRIIVNIQPMFSDYIPTHHFISKFFIDEGLIWKGEILWEKNNYNCKYCTWGSWKSPAAPYLKYSWEFIEIFCKNNLKKEGDKDSIDITDDEFKKWVYGKWNFAPERNMKQYGHDAMFPEELVKRCLKLFSYQNDIILDPFNGAGTTTKVAKQLGRRFIGIDISEKYCEVARARLKEVTNLFN
>NC_017365.1|WP_000094927.1|1052931_1053624_+|hypothetical-protein
MSSIKDIIQVYNSVVKDIDKDAQGQDDRAYGGVIRSAKGKLLEDISEAIVKIAWQNIGTKANRLEINSKKIKIPIKDSYIENIANKQVRAYILERKKDYYYGLSVDKHIFVDNQLVMGIECKNYTENAMLKRILVDFHLLKTLYPNISCYLFQLESQLGGDYSALPETPLGSKPTHSIMSYFESVNLNIITLLKGERDINQPTHKNFKPLDEQILIKTIKLMENELKAYL
>NC_017365.1|WP_001040361.1|1053658_1054558_-|polysaccharide-deacetylase
MAKEILVAYGVDIDAVAGWLGSYGGEDSPDDISRGLFAGEVGIPRLLKLFKKYHLPATWFAPGHSIETFPEQMKMIVDAGHEVGAHGYSHENPIAMSAKQEEDVLLKSIELIKDLTGKAPTGYVAPWWEFSNITNELLLKHGFKYDHSLMHNDFTPYYVRMGDSWSKIDYSLEAKDWMKPLIRGVETDLVEIPANWYLDDLPPMMFIKKSPNSFGFVSPHDIGQMWIDQFDWVYREMDYAVFSMTIHPDVSARPQVLLMHEKIIEHINKHESVRWVTFNEIADDFLKRSPRKNSGWNCI

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity
NC_017365_1	1.1|1044327|24|NC_017365|CRT	1044327-1044350	24	NZ_AP019842	Leptotrichia wadei strain JMUB3936 plasmid pJMUB3934p1, complete sequence	7368-7391	3	0.875
NC_017365_1	1.1|1044327|24|NC_017365|CRT	1044327-1044350	24	NZ_CP045419	Pseudoalteromonas sp. THAF3 plasmid pTHAF3_a, complete sequence	210069-210092	3	0.875
NC_017365_1	1.3|1044426|24|NC_017365|CRT	1044426-1044449	24	NZ_AP019842	Leptotrichia wadei strain JMUB3936 plasmid pJMUB3934p1, complete sequence	7368-7391	3	0.875
NC_017365_1	1.3|1044426|24|NC_017365|CRT	1044426-1044449	24	NZ_CP045419	Pseudoalteromonas sp. THAF3 plasmid pTHAF3_a, complete sequence	210069-210092	3	0.875
NC_017365_1	1.5|1044525|24|NC_017365|CRT	1044525-1044548	24	NZ_AP019842	Leptotrichia wadei strain JMUB3936 plasmid pJMUB3934p1, complete sequence	7368-7391	3	0.875
NC_017365_1	1.5|1044525|24|NC_017365|CRT	1044525-1044548	24	NZ_CP045419	Pseudoalteromonas sp. THAF3 plasmid pTHAF3_a, complete sequence	210069-210092	3	0.875
NC_017365_1	1.1|1044327|24|NC_017365|CRT	1044327-1044350	24	NZ_CP032539	Bacillus licheniformis strain MT-B06 plasmid unnamed1, complete sequence	45311-45334	4	0.833
NC_017365_1	1.1|1044327|24|NC_017365|CRT	1044327-1044350	24	NZ_MF996509	Bacillus glycinifermentans strain KBN06P03352 plasmid unnamed1, complete sequence	8306-8329	4	0.833
NC_017365_1	1.1|1044327|24|NC_017365|CRT	1044327-1044350	24	NZ_MF996509	Bacillus glycinifermentans strain KBN06P03352 plasmid unnamed1, complete sequence	50331-50354	4	0.833
NC_017365_1	1.1|1044327|24|NC_017365|CRT	1044327-1044350	24	NZ_CP027790	Bacillus licheniformis strain TAB7 plasmid pTAB7A, complete sequence	31308-31331	4	0.833
NC_017365_1	1.3|1044426|24|NC_017365|CRT	1044426-1044449	24	NZ_CP032539	Bacillus licheniformis strain MT-B06 plasmid unnamed1, complete sequence	45311-45334	4	0.833
NC_017365_1	1.3|1044426|24|NC_017365|CRT	1044426-1044449	24	NZ_MF996509	Bacillus glycinifermentans strain KBN06P03352 plasmid unnamed1, complete sequence	8306-8329	4	0.833
NC_017365_1	1.3|1044426|24|NC_017365|CRT	1044426-1044449	24	NZ_MF996509	Bacillus glycinifermentans strain KBN06P03352 plasmid unnamed1, complete sequence	50331-50354	4	0.833
NC_017365_1	1.3|1044426|24|NC_017365|CRT	1044426-1044449	24	NZ_CP027790	Bacillus licheniformis strain TAB7 plasmid pTAB7A, complete sequence	31308-31331	4	0.833
NC_017365_1	1.5|1044525|24|NC_017365|CRT	1044525-1044548	24	NZ_CP032539	Bacillus licheniformis strain MT-B06 plasmid unnamed1, complete sequence	45311-45334	4	0.833
NC_017365_1	1.5|1044525|24|NC_017365|CRT	1044525-1044548	24	NZ_MF996509	Bacillus glycinifermentans strain KBN06P03352 plasmid unnamed1, complete sequence	8306-8329	4	0.833
NC_017365_1	1.5|1044525|24|NC_017365|CRT	1044525-1044548	24	NZ_MF996509	Bacillus glycinifermentans strain KBN06P03352 plasmid unnamed1, complete sequence	50331-50354	4	0.833
NC_017365_1	1.5|1044525|24|NC_017365|CRT	1044525-1044548	24	NZ_CP027790	Bacillus licheniformis strain TAB7 plasmid pTAB7A, complete sequence	31308-31331	4	0.833
NC_017365_1	1.6|1044567|27|NC_017365|CRT	1044567-1044593	27	NZ_CP015757	Clostridium estertheticum subsp. estertheticum strain DSM 8809 plasmid pDSM8809, complete sequence	279-305	4	0.852
NC_017365_1	1.6|1044567|27|NC_017365|CRT	1044567-1044593	27	AY766464	Lactococcus phage TP712, complete sequence	25062-25088	5	0.815
NC_017365_1	1.6|1044567|27|NC_017365|CRT	1044567-1044593	27	NZ_CP042217	Enterococcus faecalis strain L8 plasmid pL8, complete sequence	13467-13493	5	0.815
NC_017365_1	1.6|1044567|27|NC_017365|CRT	1044567-1044593	27	AP013518	Uncultured Mediterranean phage uvMED DNA, complete genome, group G21, isolate: uvMED-CGR-U-MedDCM-OCT-S31-C15	20873-20899	5	0.815
NC_017365_1	1.6|1044567|27|NC_017365|CRT	1044567-1044593	27	MK490927	Phage Altai3, complete genome	18814-18840	5	0.815
NC_017365_1	1.6|1044567|27|NC_017365|CRT	1044567-1044593	27	MT074146	Bacteroides phage SJC03, complete genome	20128-20154	5	0.815
NC_017365_1	1.6|1044567|27|NC_017365|CRT	1044567-1044593	27	NZ_AP022840	Legionella sp. TUM19329 plasmid pTUM19329-1	131878-131904	5	0.815
NC_017365_1	1.6|1044567|27|NC_017365|CRT	1044567-1044593	27	NZ_CP040335	Bacillus cereus strain DLOU-Changhai plasmid unnamed1, complete sequence	6650-6676	5	0.815
NC_017365_1	1.6|1044567|27|NC_017365|CRT	1044567-1044593	27	NC_014534	Gloeothece verrucosa PCC 7822 plasmid Cy782202, complete sequence	255238-255264	6	0.778
NC_017365_1	1.6|1044567|27|NC_017365|CRT	1044567-1044593	27	MF775677	Lactococcus phage LP9210, complete genome	24877-24903	6	0.778
NC_017365_1	1.6|1044567|27|NC_017365|CRT	1044567-1044593	27	NC_049358	Lactococcus phage 936 group phage PhiF0139, complete genome	25078-25104	6	0.778
NC_017365_1	1.6|1044567|27|NC_017365|CRT	1044567-1044593	27	KP793113	Lactococcus phage 936 group phage PhiF.17, complete genome	25082-25108	6	0.778

1. spacer 1.1|1044327|24|NC_017365|CRT matches to NZ_AP019842 (Leptotrichia wadei strain JMUB3936 plasmid pJMUB3934p1, complete sequence) position: , mismatch: 3, identity: 0.875

caagccaaagaacaagagccaatc	CRISPR spacer
caacccaaaaaacaagagccaatt	Protospacer
*** *****.*************.

2. spacer 1.1|1044327|24|NC_017365|CRT matches to NZ_CP045419 (Pseudoalteromonas sp. THAF3 plasmid pTHAF3_a, complete sequence) position: , mismatch: 3, identity: 0.875

caagccaaagaacaagagccaatc	CRISPR spacer
caagcccatgaacaagagccaata	Protospacer
****** * ************** 

3. spacer 1.3|1044426|24|NC_017365|CRT matches to NZ_AP019842 (Leptotrichia wadei strain JMUB3936 plasmid pJMUB3934p1, complete sequence) position: , mismatch: 3, identity: 0.875

caagccaaagaacaagagccaatc	CRISPR spacer
caacccaaaaaacaagagccaatt	Protospacer
*** *****.*************.

4. spacer 1.3|1044426|24|NC_017365|CRT matches to NZ_CP045419 (Pseudoalteromonas sp. THAF3 plasmid pTHAF3_a, complete sequence) position: , mismatch: 3, identity: 0.875

caagccaaagaacaagagccaatc	CRISPR spacer
caagcccatgaacaagagccaata	Protospacer
****** * ************** 

5. spacer 1.5|1044525|24|NC_017365|CRT matches to NZ_AP019842 (Leptotrichia wadei strain JMUB3936 plasmid pJMUB3934p1, complete sequence) position: , mismatch: 3, identity: 0.875

caagccaaagaacaagagccaatc	CRISPR spacer
caacccaaaaaacaagagccaatt	Protospacer
*** *****.*************.

6. spacer 1.5|1044525|24|NC_017365|CRT matches to NZ_CP045419 (Pseudoalteromonas sp. THAF3 plasmid pTHAF3_a, complete sequence) position: , mismatch: 3, identity: 0.875

caagccaaagaacaagagccaatc	CRISPR spacer
caagcccatgaacaagagccaata	Protospacer
****** * ************** 

7. spacer 1.1|1044327|24|NC_017365|CRT matches to NZ_CP032539 (Bacillus licheniformis strain MT-B06 plasmid unnamed1, complete sequence) position: , mismatch: 4, identity: 0.833

caagccaaagaacaagagccaatc	CRISPR spacer
caagccaaagaacatgagccagat	Protospacer
************** ******. .

8. spacer 1.1|1044327|24|NC_017365|CRT matches to NZ_MF996509 (Bacillus glycinifermentans strain KBN06P03352 plasmid unnamed1, complete sequence) position: , mismatch: 4, identity: 0.833

caagccaaagaacaagagccaatc	CRISPR spacer
caagccaaagaacatgagccagat	Protospacer
************** ******. .

9. spacer 1.1|1044327|24|NC_017365|CRT matches to NZ_MF996509 (Bacillus glycinifermentans strain KBN06P03352 plasmid unnamed1, complete sequence) position: , mismatch: 4, identity: 0.833

caagccaaagaacaagagccaatc	CRISPR spacer
caagccaaagaacatgagccagat	Protospacer
************** ******. .

10. spacer 1.1|1044327|24|NC_017365|CRT matches to NZ_CP027790 (Bacillus licheniformis strain TAB7 plasmid pTAB7A, complete sequence) position: , mismatch: 4, identity: 0.833

caagccaaagaacaagagccaatc	CRISPR spacer
caagccaaagaacatgagccagat	Protospacer
************** ******. .

11. spacer 1.3|1044426|24|NC_017365|CRT matches to NZ_CP032539 (Bacillus licheniformis strain MT-B06 plasmid unnamed1, complete sequence) position: , mismatch: 4, identity: 0.833

caagccaaagaacaagagccaatc	CRISPR spacer
caagccaaagaacatgagccagat	Protospacer
************** ******. .

12. spacer 1.3|1044426|24|NC_017365|CRT matches to NZ_MF996509 (Bacillus glycinifermentans strain KBN06P03352 plasmid unnamed1, complete sequence) position: , mismatch: 4, identity: 0.833

caagccaaagaacaagagccaatc	CRISPR spacer
caagccaaagaacatgagccagat	Protospacer
************** ******. .

13. spacer 1.3|1044426|24|NC_017365|CRT matches to NZ_MF996509 (Bacillus glycinifermentans strain KBN06P03352 plasmid unnamed1, complete sequence) position: , mismatch: 4, identity: 0.833

caagccaaagaacaagagccaatc	CRISPR spacer
caagccaaagaacatgagccagat	Protospacer
************** ******. .

14. spacer 1.3|1044426|24|NC_017365|CRT matches to NZ_CP027790 (Bacillus licheniformis strain TAB7 plasmid pTAB7A, complete sequence) position: , mismatch: 4, identity: 0.833

caagccaaagaacaagagccaatc	CRISPR spacer
caagccaaagaacatgagccagat	Protospacer
************** ******. .

15. spacer 1.5|1044525|24|NC_017365|CRT matches to NZ_CP032539 (Bacillus licheniformis strain MT-B06 plasmid unnamed1, complete sequence) position: , mismatch: 4, identity: 0.833

caagccaaagaacaagagccaatc	CRISPR spacer
caagccaaagaacatgagccagat	Protospacer
************** ******. .

16. spacer 1.5|1044525|24|NC_017365|CRT matches to NZ_MF996509 (Bacillus glycinifermentans strain KBN06P03352 plasmid unnamed1, complete sequence) position: , mismatch: 4, identity: 0.833

caagccaaagaacaagagccaatc	CRISPR spacer
caagccaaagaacatgagccagat	Protospacer
************** ******. .

17. spacer 1.5|1044525|24|NC_017365|CRT matches to NZ_MF996509 (Bacillus glycinifermentans strain KBN06P03352 plasmid unnamed1, complete sequence) position: , mismatch: 4, identity: 0.833

caagccaaagaacaagagccaatc	CRISPR spacer
caagccaaagaacatgagccagat	Protospacer
************** ******. .

18. spacer 1.5|1044525|24|NC_017365|CRT matches to NZ_CP027790 (Bacillus licheniformis strain TAB7 plasmid pTAB7A, complete sequence) position: , mismatch: 4, identity: 0.833

caagccaaagaacaagagccaatc	CRISPR spacer
caagccaaagaacatgagccagat	Protospacer
************** ******. .

19. spacer 1.6|1044567|27|NC_017365|CRT matches to NZ_CP015757 (Clostridium estertheticum subsp. estertheticum strain DSM 8809 plasmid pDSM8809, complete sequence) position: , mismatch: 4, identity: 0.852

aataaggaggaaaaacaagaaaaaacg	CRISPR spacer
agtaaggaggaaaaacaagtgaaaaag	Protospacer
*.***************** .**** *

20. spacer 1.6|1044567|27|NC_017365|CRT matches to AY766464 (Lactococcus phage TP712, complete sequence) position: , mismatch: 5, identity: 0.815

aataaggaggaaaaacaagaaaaaacg	CRISPR spacer
aataaggagaaaaaacatgaaaaacaa	Protospacer
*********.******* ******  .

21. spacer 1.6|1044567|27|NC_017365|CRT matches to NZ_CP042217 (Enterococcus faecalis strain L8 plasmid pL8, complete sequence) position: , mismatch: 5, identity: 0.815

aataaggaggaaaaacaagaaaaaacg	CRISPR spacer
cataaggaggaaaaacatgacaaaaaa	Protospacer
 **************** ** **** .

22. spacer 1.6|1044567|27|NC_017365|CRT matches to AP013518 (Uncultured Mediterranean phage uvMED DNA, complete genome, group G21, isolate: uvMED-CGR-U-MedDCM-OCT-S31-C15) position: , mismatch: 5, identity: 0.815

aataaggaggaaaaacaagaaaaaacg	CRISPR spacer
gatgagcaggaaaaacaagaaaaacct	Protospacer
.**.** ***************** * 

23. spacer 1.6|1044567|27|NC_017365|CRT matches to MK490927 (Phage Altai3, complete genome) position: , mismatch: 5, identity: 0.815

aataaggaggaaaaacaagaaaaaacg	CRISPR spacer
gttaaagaggaaaaacaagaagaaaag	Protospacer
. ***.***************.*** *

24. spacer 1.6|1044567|27|NC_017365|CRT matches to MT074146 (Bacteroides phage SJC03, complete genome) position: , mismatch: 5, identity: 0.815

aataaggaggaaaaacaagaaaaaacg	CRISPR spacer
aagaaggagcaaaaacaagaaaatata	Protospacer
** ****** ************* *..

25. spacer 1.6|1044567|27|NC_017365|CRT matches to NZ_AP022840 (Legionella sp. TUM19329 plasmid pTUM19329-1) position: , mismatch: 5, identity: 0.815

aataaggaggaaaaacaagaaaaaacg	CRISPR spacer
caaactggggaaaaacaagaaaaaacg	Protospacer
 * *  *.*******************

26. spacer 1.6|1044567|27|NC_017365|CRT matches to NZ_CP040335 (Bacillus cereus strain DLOU-Changhai plasmid unnamed1, complete sequence) position: , mismatch: 5, identity: 0.815

aataaggaggaaaaacaagaaaaaacg	CRISPR spacer
aggaaagaagaaaaacaagaaaaaaca	Protospacer
*. **.**.*****************.

27. spacer 1.6|1044567|27|NC_017365|CRT matches to NC_014534 (Gloeothece verrucosa PCC 7822 plasmid Cy782202, complete sequence) position: , mismatch: 6, identity: 0.778

aataaggaggaaaaacaagaaaaaacg	CRISPR spacer
aataaagaggaaaaacaagaacacctt	Protospacer
*****.*************** *  . 

28. spacer 1.6|1044567|27|NC_017365|CRT matches to MF775677 (Lactococcus phage LP9210, complete genome) position: , mismatch: 6, identity: 0.778

aataaggaggaaaaacaagaaaaaacg	CRISPR spacer
gataaagaggaaaaacaacaaaaagaa	Protospacer
.****.************ *****. .

29. spacer 1.6|1044567|27|NC_017365|CRT matches to NC_049358 (Lactococcus phage 936 group phage PhiF0139, complete genome) position: , mismatch: 6, identity: 0.778

aataaggaggaaaaacaagaaaaaacg	CRISPR spacer
gataaagaggaaaaacaacaaaaagaa	Protospacer
.****.************ *****. .

30. spacer 1.6|1044567|27|NC_017365|CRT matches to KP793113 (Lactococcus phage 936 group phage PhiF.17, complete genome) position: , mismatch: 6, identity: 0.778

aataaggaggaaaaacaagaaaaaacg	CRISPR spacer
gataaagaggaaaaacaacaaaaagaa	Protospacer
.****.************ *****. .