CRISPRimmunity

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Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Self targeting spacer number	Target MGE spacer number	Prophage number	Anti-CRISPR protein number
NC_021280	Spiroplasma chrysopicola DF-1, complete sequence	1 crisprs	DEDDh	0	1	1	0

Results visualization

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CRISPR-Cas detection and classification

Crispr_ID: NC_021280_1

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

NC_021280_1

366537-366615

Orphan

Consensus_repeat	Method
TTTTTAAAATGATTGCTAAAAAAATTA	CRISPRCasFinder

1 spacers

DEDDh

The CRISPR arrays of NC_021280_1

>merge|NC_021280|1|366537-366615|CRISPRCasFinder
TTTTTAAAATGATTGCTAAAAAAATTAAGATTAATTAATTTACTATTTTAAAATTTTTAATTAATTTTTAAAATAATTG

>NC_021280|1|1|366537-366615|CRISPRCasFinder
TTTTTAAAATGATTGCTAAAAAAATTA	AGATTAATTAATTTACTATTTTAAA
ATTTTTAATTAATTTTTAAAATAATTG

Protein	Signature genes	Signature genes Name	Protein_function
NC_021280.1\|WP_016338749.1\|371212_372208_+\|ABC-transporter-ATP-binding-protein	unknown	unknown	gnl\|CDD\|224054
NC_021280.1\|WP_016338750.1\|372375_373974_+\|MATE-efflux-family-protein	unknown	unknown	gnl\|CDD\|240548
NC_021280.1\|WP_016338746.1\|367077_367791_+\|hypothetical-protein	unknown	unknown	unknown
NC_021280.1\|WP_016338745.1\|366670_367030_+\|hypothetical-protein	unknown	unknown	unknown
NC_021280.1\|WP_016338739.1\|359045_360413_+\|glycine--tRNA-ligase	unknown	unknown	gnl\|CDD\|235240
NC_021280.1\|WP_016338736.1\|352908_357264_+\|PolC-type-DNA-polymerase-III	DEDDh	cd06127_DEDDh_CAS-I	gnl\|CDD\|234767
NC_021280.1\|WP_016338735.1\|351534_352887_+\|site-2-protease-family-protein	unknown	unknown	gnl\|CDD\|100084
NC_021280.1\|WP_041611930.1\|376209_376494_+\|50S-ribosomal-protein-L27	unknown	unknown	gnl\|CDD\|235464
NC_021280.1\|WP_016338738.1\|358232_358991_+\|DNA-repair-protein-RecO	unknown	unknown	gnl\|CDD\|273175
NC_021280.1\|WP_016338741.1\|362353_363682_+\|sigma-70-family-RNA-polymerase-sigma-factor	unknown	unknown	gnl\|CDD\|235640
NC_021280.1\|WP_016338753.1\|375892_376207_+\|ribosomal-processing-cysteine-protease-Prp	unknown	unknown	gnl\|CDD\|381747
NC_021280.1\|WP_016338737.1\|357336_358230_+\|GTPase-Era	unknown	unknown	gnl\|CDD\|234624
NC_021280.1\|WP_016338744.1\|365119_366004_-\|4-hydroxy-3-methylbut-2-enyl-diphosphate-reductase	unknown	unknown	gnl\|CDD\|376776
NC_021280.1\|WP_016338742.1\|363695_364358_+\|SAM-dependent-methyltransferase	unknown	unknown	gnl\|CDD\|378972
NC_021280.1\|WP_016338748.1\|369356_371213_+\|ABC-transporter-permease	unknown	unknown	gnl\|CDD\|224196
NC_021280.1\|WP_016338751.1\|373988_375095_-\|GNAT-family-N-acetyltransferase	unknown	unknown	gnl\|CDD\|366181
NC_021280.1\|WP_016338743.1\|364359_365109_+\|Nif3-like-dinuclear-metal-center-hexameric-protein	unknown	unknown	gnl\|CDD\|376614
NC_021280.1\|WP_016338747.1\|367867_369247_+\|DEAD/DEAH-box-helicase	unknown	unknown	gnl\|CDD\|223587
NC_021280.1\|WP_016338752.1\|375320_375890_+\|50S-ribosomal-protein-L21	unknown	unknown	gnl\|CDD\|235510
NC_021280.1\|WP_016338740.1\|360434_362348_+\|DNA-primase	unknown	unknown	gnl\|CDD\|235551

Protein	Function_ID	Function_description	E-value
NC_021280.1\|WP_016338749.1\|371212_372208_+\|ABC-transporter-ATP-binding-protein	gnl\|CDD\|224054	COG1131, CcmA, ABC-type multidrug transport system, ATPase component [Defense mechanisms].	7.28422e-72
NC_021280.1\|WP_016338750.1\|372375_373974_+\|MATE-efflux-family-protein	gnl\|CDD\|240548	cd13143, MATE_MepA_like, Subfamily of the multidrug and toxic compound extrusion (MATE)-like proteins similar to Streptococcus aureus MepA. The integral membrane proteins from the MATE family are involved in exporting metabolites across the cell membrane and are responsible for multidrug resistance (MDR) in many bacteria and animals. This subfamily includes Streptococcus aureus MepA and Vibrio vulnificus VmrA and functions most likely as a multidrug efflux pump.	8.6557e-45
NC_021280.1\|WP_016338739.1\|359045_360413_+\|glycine--tRNA-ligase	gnl\|CDD\|235240	PRK04173, PRK04173, glycyl-tRNA synthetase; Provisional.	0
NC_021280.1\|WP_016338736.1\|352908_357264_+\|PolC-type-DNA-polymerase-III	gnl\|CDD\|234767	PRK00448, polC, DNA polymerase III PolC; Validated.	0
NC_021280.1\|WP_016338735.1\|351534_352887_+\|site-2-protease-family-protein	gnl\|CDD\|100084	cd06163, S2P-M50_PDZ_RseP-like, RseP-like Site-2 proteases (S2P), zinc metalloproteases (MEROPS family M50A), cleave transmembrane domains of substrate proteins, regulating intramembrane proteolysis (RIP) of diverse signal transduction mechanisms. In Escherichia coli, the S2P homolog RseP is involved in the sigmaE pathway of extracytoplasmic stress responses. Also included in this group are such homologs as Bacillus subtilis YluC, Mycobacterium tuberculosis Rv2869c S2P, and Bordetella bronchiseptica HurP. Rv2869c S2P appears to have a role in the regulation of prokaryotic lipid biosynthesis and membrane composition and YluC of Bacillus has a role in transducing membrane stress. This group includes bacterial and eukaryotic S2P/M50s homologs with either one or two PDZ domains present. PDZ domains are believed to have a regulatory role. The RseP PDZ domain is required for the inhibitory reaction that prevents cleavage of its substrate, RseA.	5.36928e-39
NC_021280.1\|WP_041611930.1\|376209_376494_+\|50S-ribosomal-protein-L27	gnl\|CDD\|235464	PRK05435, rpmA, 50S ribosomal protein L27; Validated.	1.19459e-50
NC_021280.1\|WP_016338738.1\|358232_358991_+\|DNA-repair-protein-RecO	gnl\|CDD\|273175	TIGR00613, DNA_repair_protein_RecO, DNA repair protein RecO. All proteins in this family for which functions are known are DNA binding proteins that are involved in the initiation of recombination or recombinational repair. [DNA metabolism, DNA replication, recombination, and repair].	1.30877e-27
NC_021280.1\|WP_016338741.1\|362353_363682_+\|sigma-70-family-RNA-polymerase-sigma-factor	gnl\|CDD\|235640	PRK05901, PRK05901, RNA polymerase sigma factor; Provisional.	5.50948e-131
NC_021280.1\|WP_016338753.1\|375892_376207_+\|ribosomal-processing-cysteine-protease-Prp	gnl\|CDD\|381747	cd16332, Prp-like, ribosomal-processing cysteine protease Prp and similar proteins. This model represents a family of cysteine proteases that include members found to cleave the N-terminus extension of ribosomal subunit L27 in eubacteria. Proteins in this family are distinguished by a pair of invariant histidine and cysteine residues with conserved spacing that form the classic catalytic dyad of a cysteine protease. Dependence of Staphylococcus aureus on L27 cleavage by Prp makes the enzyme a target for antibiotic development.	7.86968e-31
NC_021280.1\|WP_016338737.1\|357336_358230_+\|GTPase-Era	gnl\|CDD\|234624	PRK00089, era, GTPase Era; Reviewed.	1.11269e-166
NC_021280.1\|WP_016338744.1\|365119_366004_-\|4-hydroxy-3-methylbut-2-enyl-diphosphate-reductase	gnl\|CDD\|376776	pfam02401, LYTB, LytB protein. The mevalonate-independent 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway for isoprenoid biosynthesis is essential in many eubacteria, plants, and the malaria parasite. The LytB gene is involved in the trunk line of the MEP pathway.	5.86489e-91
NC_021280.1\|WP_016338742.1\|363695_364358_+\|SAM-dependent-methyltransferase	gnl\|CDD\|378972	pfam12847, Methyltransf_18, Methyltransferase domain. Protein in this family function as methyltransferases.	1.14141e-58
NC_021280.1\|WP_016338748.1\|369356_371213_+\|ABC-transporter-permease	gnl\|CDD\|224196	COG1277, NosY, ABC-type transport system involved in multi-copper enzyme maturation, permease component [General function prediction only].	2.4603e-09
NC_021280.1\|WP_016338751.1\|373988_375095_-\|GNAT-family-N-acetyltransferase	gnl\|CDD\|366181	pfam00583, Acetyltransf_1, Acetyltransferase (GNAT) family. This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.	1.12122e-09
NC_021280.1\|WP_016338743.1\|364359_365109_+\|Nif3-like-dinuclear-metal-center-hexameric-protein	gnl\|CDD\|376614	pfam01784, NIF3, NIF3 (NGG1p interacting factor 3). This family contains several NIF3 (NGG1p interacting factor 3) protein homologs. NIF3 interacts with the yeast transcriptional coactivator NGG1p which is part of the ADA complex, the exact function of this interaction is unknown.	1.88269e-58
NC_021280.1\|WP_016338747.1\|367867_369247_+\|DEAD/DEAH-box-helicase	gnl\|CDD\|223587	COG0513, SrmB, Superfamily II DNA and RNA helicases [DNA replication, recombination, and repair / Transcription / Translation, ribosomal structure and biogenesis].	4.88402e-109
NC_021280.1\|WP_016338752.1\|375320_375890_+\|50S-ribosomal-protein-L21	gnl\|CDD\|235510	PRK05573, rplU, 50S ribosomal protein L21; Validated.	4.97699e-45
NC_021280.1\|WP_016338740.1\|360434_362348_+\|DNA-primase	gnl\|CDD\|235551	PRK05667, dnaG, DNA primase; Validated.	2.66971e-145

>NC_021280.1|WP_016338744.1|365119_366004_-|4-hydroxy-3-methylbut-2-enyl-diphosphate-reductase
MNVIKVTPRGYCLGVVKSIKWAKDAAKQYHDKEIFMLGYLVHNQHVVKEIIDLGIIPINDYKQNRLDLLKNLPNNSVVILSAHGSDERIKTLAAQKNIIIVDTECEWVTATKDLIREFLAKKDYEIIFIGKTHHPETNAILSISPLIHLVTTVTEIEPIIPQLDHTKKILITNQTTLSKIDIEDIVLNIKNQLPERIIEFKNDLCNATLERQNAVLALDSDQIDLLLVVGDKRSNNTLKLVEMGERKNILSYRINDKNDIKDEWLKNINTVAVTAGASTPSNIQLEVIKYLEEQ
>NC_021280.1|WP_016338743.1|364359_365109_+|Nif3-like-dinuclear-metal-center-hexameric-protein
MQAKEIFKIIEADFPLRWAEKWDHCGRQYGKKTIEVDKVLVALDLTTEVINEAIKKKVNLIITHHPFVFQKLKKEQKVAYKNKIFKLLDENNITVYATHTNFDGKMNELILSILKGENIRNFTKDNHILKVATVKTTANLISETLKKLFEINVIQHNLSDFNQKIKTVAICTGAGGSYLNILPSEIDLFITGEVKWNEWVIANEKNLNVICFNHYMENYYTKIFTQYLAEKFPALTVDAYEIKNIINYR
>NC_021280.1|WP_016338742.1|363695_364358_+|SAM-dependent-methyltransferase
MTKLSERLLLIAKQVNDKDIICDIGTDHGLVPIYLAKGNLITKAYACDIAPKPLQQAIDNIKKYDVEEVITPVLADGLNGVKGIKIDSCIIAGMGSSTIFDILQEDQTTIGRYIFCPNDDPYLLRQWVKEQKYFIEKELLIKENNYIYEIIVVNKVAGHKVKSQKDIFFGVLLRKEKNALFNEKWLLKSDYYQTLLGRLPEKSQRYKELKKKLKLINKMI
>NC_021280.1|WP_016338741.1|362353_363682_+|sigma-70-family-RNA-polymerase-sigma-factor
MELTKKEVKDMQTFDEFKEYVSKYLEENNNEIEQEKLLSLINDHFEIDDNDVDEYFEELLSNGVDFSDVTGIDEVEEEPEKPVKKAAEEKIKYKVGGISNETKIQDIIKAYFNVLGTSKILTREEEIKYAKMLESKDEEERKYGRDKLITSNLKLVVSVARKHLNRGLDFSDLIEEGNIGLMKAVDKFDYKRGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLTRIERQLTQELGREPTFDEIAERMGQGMTGEKVREIKRLSIEPVSLEKPIGDEDDTHFGDFVDDKDIFTPDEYAEKESLREVIDEVFHEILSAREEKVIRMRFGILPTKLRTVLRLAKECEDETFPELVQTIKTLDIHYDTPIEKVQSRKNKLIDKHLSKYDSPKTLEEVGREFKVTRERIRQIEAKTIRKFKPNQATSKAKVLKDFFKG
>NC_021280.1|WP_016338740.1|360434_362348_+|DNA-primase
MALISNEKIESIRSKVNIVEILSEYLQLEKRGRNFWAVCPFHQDSHPSMSISPEKQIYRCFACSAGGNVFTFLQEYKNISFIEALKIVAEKANVSLTELQNYQEVSRYSEEDKKIFAINELTKTFFMNNLQTKKGLPAKQYLEERDISNEDILAFDLGYADSNSQSLYNFLLKKGFTINEIEQAGLVNINGKGQIYDYFNDRVMFPIHDDENNIIGFSGRLVQEKTNLPKYLNTLETKVFKKEQVMYNFYRAKPFIKKSGNLILLEGYMDIISLNRMGIKNTVALMGTNLSDYHITTIKKVTPECIIFLDGDLPGVKASLKAAAKLLVNNFKVKVVYNETGKDPDELVKAGEEEFIKKIIVGANYPVNFAINYFMDKYNLEDANELAEFLTIVGNLVKVIPDPIEKELSINNLAKVTKLSPTAIATKIEHLKGNLKPVSTKGPVKPPGLVTKAPEPTGASNYFIPDEPSTVEPINPSAINEIILTWKPYRIKNLKRYTLAEEKMILQLLFSRQAMNFYQLKIGALNNNNYRLIANDIIDYYHRHLSAESVNINMLCDEINDGQLNAILMTILNKSTLKTKYNKKELEDYAILINDYANEKEIEILRKKMTEATTLEEKQVISNEVDMMNQRLKFKKG
>NC_021280.1|WP_016338739.1|359045_360413_+|glycine--tRNA-ligase
MKYSLEEVVNHLKTQGFVFPGSEIYGGLANAWDFGPLGIEIVRKLKNLWWQFFVTKSKYNVGLDSAILMNNNVWKASGHLGNFSDPLLDCKKCQARMRADKLIEEKYPDINCGGWDNKQLTDFINTEQLACPHCGAHDFTDIRQFQLMFQTNQGVLQDEKSVVYLRPETAQGIFVNFKNIQRSLRKKLPFGVGQIGKSFRNEITPGNFIFRTREFEQMELEFFYDPADQTDWFTYWVQQVTIFLTKLGLKSENYVLREHDQKELAHYAKRTIDIEYKFSFGQGELWGIADRGDFDLKSHNDYSHQNLSYLNPETNEKIIPHVIEPSVGVGRLLLALLADSYEVEKIGENDSRVVLKLSPLLSPYQIAIIPLSKQLNEQAYHLYETLLTDFDCTYDETGNIGKRYRRQDAIGTPYCITYDFDSLTDNAVTVRDRDTMKQIRIAISELPAYLKQHLK
>NC_021280.1|WP_016338738.1|358232_358991_+|DNA-repair-protein-RecO
MANNLQGLIISKINYNDYDQIITIFSREEGKLSFYAPGVRKAISKNQFSLQLFATSEFELFLSYHKNKVSKLKTGVLIKDRNLLAKDYEDYLVATLMVEVLDQAIEERTADDELYNLITTSLDCLLNNSDNLIIVIFYLFKMLKWYGLRWDFGKCQRCYKKEGIKTISFIDQGLICRGCITEKDYLFSIGLIKEIILWQDEQFTIATLASKTFVTNQELMLLFRMLCEYYLNVVGIFSYSIKEIAEKSIYFK
>NC_021280.1|WP_016338737.1|357336_358230_+|GTPase-Era
METKSGFVAIVGRPNVGKSTLLNSLLAQKVAIVSPKAQTTRNRIQGIYNDKEAQIVFMDTPGIHSAHHAMGKFMNKVALSSTKAAEVILFLVPANEYIGQNDHFILNALKERNVPVILVITKIDLVTNDQLLEKIAAWKGLYQFSEIIPISALKNNNTDRLLTILKSHLEVGPKYYPDEMISDQPELFLIREIIREKILFLTEEEVPHSVAILIDKLEEKPNLLKIIASICVERDSQKKIIIGKGGQLIKKIGTQAREELEQILNTKIYLELFVKVVNKWRDKPSMIARLGYNKESY
>NC_021280.1|WP_016338736.1|352908_357264_+|PolC-type-DNA-polymerase-III
MDQQLERLFLANNLKFDEQYFIGAKIIKSEYSTTDKVFKVVIVINDFLPVDLLLTLETTLLNNPQMPTKVGFEVLKRNYQLADIFHYLEYIRVNKSEVKQSFFGKLSNDRFTLVGQELIIAVYSDSEKKLVTEHSIYYQKQLRRYGFADLTLVINQIETVNNILELNEQELRKYQSSALAYDEKQQDNIPPALVKPKVGGYTKTNPLSPTYKKIVEIDQDTPNITIHGQVFNKARVQTAAKKYIYTIQITDKTDSIGVKFFSRNEQPDMAIEELKNGDWISVFGDIRYDTYANEQMFFAKKITKLEEKNEERQDTALKKRVELHLHTKMSIMDGLIEIPSLFKTLNTWEHCAVAITDHINVQAFPEVYNTSLKYPNLKVIYGVEADILENKVWYVKNPHHHNLKTAKYVIFDLETTGLSSEYDEIIEFGAVIYDSNTGEKKVVNHLFKPTTPVSSFTTELTGITNDLLVDKPSFIDSIDDLLAYFKDAILIAHNAEFDMGFIQSWLQKVNRPIIDNTVIDTLQLARILEPNLKNYRLGTIARCYNVIYNEEVAHRGDYDANVLSEIYEYQLRKLTSDYNITFDDEIDQIHQPLIYNKLRPKHVTILAKNQQGLKDLFTLVTEAHTKYFYGSPKLLKDCLIPVRANLLIGSACVNGEIFELARNKSKEELRATIAFYDYIEVQPLSVYKHLLQMGDLTQERLEIIVKDIISIAKELNKIVVATGDAHYLNPEDKIFREVYIHAKGIGGKAHPLYDYKQRVKDYPEQFFRTTDEMLNEFQFLQDEKLAYELVVTNSNLIAEQVEKVQVIKDKLYTPKIEGSDELLRTLCYKNAHQIYGEQLPPIVAERLERELSAIIKHGFAVIYWIAHKLVDKSLQDGYLVGSRGSVGSSFVATMSNITEVNPLQPHYLCKECCYSEFITDGSVRCGYDLPNKPCPKCQQQLFGEGHDIPFETFLGFEADKVPDIDLNFSGEYQPIAHDFTKEMFGEKSVYRAGTISTVAEKSAYGYVKSYFELKNALHLKRKAEIERIAKGCEGVKRTTGQHPGGIVVIPNDFIVEEFTPVNFPADDVTSTWLTTHFDFHAIHDNVLKLDILGHVDPTALRMLQDLTGVDPKTIPTNDLTVLSLFCNLDSLKITPDDLNGEKTGAIGIPEFGTTFVRKMLLDTKPQSFADLVQISGLSHGTDVWLGNAQELIRNQGVKISEVIGCRDDIMVNLIYKGLPAKTAFKIMEDVRKGKGLLPEYQALMLEHNVEQWYIDSCNKIKYMFPKAHATAYVLMAWRVAWFKINYPTEYYATYFSTRTDVFDIKTILKGPETIKQVVQDIQQRMDIKDFNFQNKPSQKEKDLIPVYEVALEMYGRGIKMLNISLEDSDNKNFKIVKDENNNKMILPPFSAIDGLGEAVGESIIRARLEKPFISISDLQKRTNITKAHMQSFEDLGTLNHLSLDDQISFNF
>NC_021280.1|WP_016338735.1|351534_352887_+|site-2-protease-family-protein
MSVGMIILGFVIGIIILLILVTIHEFAHFIIAKLAGAYVYEFAIGFGPKLFSWGKKETKYSLRLIPFGGYVYIASELADPPKGREDEVIPEHRKMENIKRWKKMLFIVAGAIMNFFIAVFIFTILFASLGHKPNDMTYWGATYSPQGVAYQAFQGTPNITEVNQEIVILDYWLGTDKTQIEQAALDYNNCLAQGNDNCVDSPGVTVSHLSKNNQIPDYTTTVYNFIDVLKKQYDKNPEHNDAIVFRIRTVNAHQQFSGPLLEPTITSAVAFDQTKGVYTVGIAAPNRIFTSTWQGYSYGWKETFTQSVSILKSFGLLFTGQWGQLSGPVGIAKTMSSILDNGAVPFFLYVAMLSANLFVLNLIPIPPLDGYKFLETAVEGTIISGKKINGRYKMWYYRKDEVKLAQAKIKYEEINPNWHLPNKTKLIINITGAVLFILLFIGITIKDIFF
>NC_021280.1|WP_016338745.1|366670_367030_+|hypothetical-protein
MAAIKEKQKMTKEMFDKIQRAKWMEIKKEAIAKATPAMLISINRKTGVVENKNFKDYREKDGSAASGIFRAEDLIADRLGVKPSSVKKTNAAKKSNTIPAEVITKAKPAKKVTTKKQKN
>NC_021280.1|WP_016338746.1|367077_367791_+|hypothetical-protein
MKSFGKWGISIIWIIPIIIFILCLTFLALPYSGYYWDNNSDLPTNINQIHTFSGSGFKLIQQLGFPTFLKLANTGTFAWGYNATFVFDGKTYNILEWAQSHSNEGGVIDWLDMELTTGLNFAALVGGLLTGIIIAVPVLVLVAIWVTIKLHRAMICQHSIEYIDAKNSLKKANKESKKDIKKAKKTFKKGIINKEKLIDVEKYWAEIIIERNNIFIREKAFYEEQYNDKKLLLKTKK
>NC_021280.1|WP_016338747.1|367867_369247_+|DEAD/DEAH-box-helicase
MGQFSNFGLKKFLNDGLIDLKFLEPTLVQEQVIPLLQKHQNVICLANTGTGKSHSFILPILNNLDYQLNRIQSVIVTPTRELAKQIYDNIRFFKTYQPELQVGCFIGGEDIKRQIEQLGKSQPQIIVVTPTRLKDLFLADALNFGKLSSFIIDECDMIFDLGFIDDVDYILSKVNNNVKVSVFSATIAESLKPFLLKYLKNPHFIDVNKTTVTNQNIEHVLIPTKHQDRGKVLLKVLAAINPYLCLIFVNRREEIPGYFDLLRENGYSVCQLHAGLEPRLRTQMVKRIKNLEFKYVIATDVAARGIDLDGVSHVISIDLPNNLEYYIHRSGRTGRGQYTGKSYVLYDTKNLSFVKQLEQWGITFEYQKWNSNNELVTVNLEAPRKKYQPQTTTNEVNKIINRYKTKNNNKKIKPGYKKKRKEEIDNLTKQIRREHIKNSIKKIKKEKAKERGKKLFDKE
>NC_021280.1|WP_016338748.1|369356_371213_+|ABC-transporter-permease
MNHSVLKNKNQKTSKFQEKLKNAFSLKVFRYSLIKMLKSPSTYVLLAITMVTNANLVLTFTGLSRSYGINLNEDINQQLSFYLWIFFIFYFSFSAMYIGFKSVQIIRDELDDGTLLIMASLPITRFKMIFEKWLSLQVICLSYAIIVLFIPPLFGYTTGEVGQLLTNAIYKALPYMFLTGIIIQFLLSSVAILLSLIIGSKGVIGVLFILGFLSLIGALGPFIYNATNTSNPSSNFLLSTSDSAKKKISLNNTNKNVFKNMTDIRGWDIWNLSSNGLTIKFPNDSRPNFALKYDLTNINEETEAIIAKKPNVSSEEYRTYILANASDYPLMNNLVSNHELTIDSVSLPKYIGGQNFTILENDNLGINLLSDFYENVKTKKPVTLSWKNNVIGDLGVKNFDTTNITTPENKEILQLINEWYQNELFTPNSKNYSVNNLIDLDDLSKQITKENLNGLFYLVKNSLTPPSSREIYFNDLSLNTINYFTNNLLQEIWTPILSFGKTNNDIDIANKEIDKIKQQELSYRLMGYLNIWQQWMVMWTGNKGNSGFLLPLNNFLTSELYPVKYQTGSDSFTTYSIGANFAAPVEIVNLPAMISVYTIFSIGIAGLSVWSITRKDFV
>NC_021280.1|WP_016338749.1|371212_372208_+|ABC-transporter-ATP-binding-protein
MMKALFKKKVNNINPNPTLAIEIKNFTKKFKKFTAVDNINISVKKGKIHGFIGPNGSGKTTTIKSLIGAIIATNGEFYINGLVATSVDAKKAIGYIPENARFPKHLKSLEYLAEMGYLRGIPYKEAKTKARKILVSLNLIKFKSKNPNTFSSGMKKKILLAQALMADPEVLILDEPAANLDPTARQELFNDLIKLRDQGKTILICSHILSELQDIADEITILNYGKIVYYGEVINASHNYFEFTVMEPEMLTKLGQEIKKLGYEIIHQFADSYVIKLNDTKNEIKRIAFAAFEQKIILIGIQPYFTNITKIYDDVVFSNNKNFGQASALVS
>NC_021280.1|WP_016338750.1|372375_373974_+|MATE-efflux-family-protein
MTTVLTKREQTLRYEKPWKTILIFCLPTVFLMIVQGLYNILDKSLAQVFAAPDAIHNQYYIDMYNELKGTLVNEIPLADMRSFINVATQYASQTYNLLWAFSVMFGMGCAMNFSIAFGKRDTHKMQELSGNAFAVTTLFSILISFIIFSIVYPGWGAVFITSQMGSHFNPITQALCWNYTIPMLLATPMMFLSYYFISLIRSEGRMKWVMAMVVSSLAINAGVAIFFMRVVHLEMAGAMLGTVFSWIVQVIWGFFIVFKTKDSYAKFTWANLVNIKLNNIWQFMRAGFPNFIINGAIVITSYVSTMLVVQLPNQDFNDGVSVLQELYSSMVPWMTLILSAGIGMTQGSRTILAYNFGAEKYQRIWQILKRVSIIIIAWFVLMLIVIISFGQQMMELFAFPHEYAVQYRWWIVINFATYPFCGFTYIALTLFQGINRSFLATLTSSLRTVIVILPLIGIGYAVSVKTGNPIFFYIFIGLTDLCSAAIIIPILAYFWFKYQTKLVDKPDNYEHDQISESHHLKKTKKNLANPKE
>NC_021280.1|WP_016338751.1|373988_375095_-|GNAT-family-N-acetyltransferase
MRRKKYRLNYKVKRMFKFKGAVEATQLAQNFVRYITNPFIDQTLKNELIDHENEIYMSDIISDEGAKYTIAISTQLDYQEDAERVKELVQDYVDNDLDFIWYTIGDKIDIQENQFFESIGLVQNDNLIGMIMDVKKWKKQQKKVEDQTIKFRRVNNKKRLNDFSSILKAALGPKSWDYLFYKTLLKLNKDDDIAEIDLLYKNDQPAVTGNIYFEKDLVIIDDIATHPDFRHQGLAKKMINHLLHRAVSHGYDLVGLIATPQGYNLYRKMGFRPIKLYLTEYVTKTSKSNLEQIATKISKGKLKKLQVVKKDSLLAMVNDLVCENCQEKIEDPQYITTLTNLPNFSINNYHKKCYHFSAKERWVVISEQ
>NC_021280.1|WP_016338752.1|375320_375890_+|50S-ribosomal-protein-L21
MFAIIKTGGKQIKVSQGDEIFVEMLTGVEGDKVRFDEVLMIDSKIGSPTIKGAAVTGVIMKQGKQKKIVVFHYKPKKNVHKKYGHRQPYTKVKIEEISLTGASASKAAPAASPAPAAPKAAPTVKPAAVVKPVGEKVTAVKPATEPKPAGAKPAAPKTTTTTAKPKSPSTTAAKTVATKKPAENKNTDK
>NC_021280.1|WP_016338753.1|375892_376207_+|ribosomal-processing-cysteine-protease-Prp
MIKINFIKTENKIKKVEITGHALQAESGKDLVCAAVTAISTGTLNAIDQIKEHSCNFTIDDGLIVIEVFSDDDILQIILQTMYYQLLTIYQQYQTYIQLKEVEQ
>NC_021280.1|WP_041611930.1|376209_376494_+|50S-ribosomal-protein-L27
MKFLLGLQLFASKKGVGSTKNGRDSHSKRLGAKRADGQAIHAGSIIYRQRGTKVHPGVNVGRGGDDTLFALIDGVVKFEKFGKNKTKASVYPKN

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Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity
NC_021280_1	1.1\|366564\|25\|NC_021280\|CRISPRCasFinder	366564-366588	25	NC_006872	Clostridium perfringens plasmid pBCNF5603 DNA, complete sequence	32460-32484	2	0.92
NC_021280_1	1.1\|366564\|25\|NC_021280\|CRISPRCasFinder	366564-366588	25	NZ_CP013044	Clostridium perfringens strain JP55 plasmid pJFP55J, complete sequence	36906-36930	2	0.92
NC_021280_1	1.1\|366564\|25\|NC_021280\|CRISPRCasFinder	366564-366588	25	NZ_CP011154	Bacillus cereus strain CMCC P0011 plasmid pRML05, complete sequence	148362-148386	3	0.88
NC_021280_1	1.1\|366564\|25\|NC_021280\|CRISPRCasFinder	366564-366588	25	NZ_CP011152	Bacillus cereus strain CMCC P0021 plasmid pRML04, complete sequence	398529-398553	3	0.88
NC_021280_1	1.1\|366564\|25\|NC_021280\|CRISPRCasFinder	366564-366588	25	MG945592	UNVERIFIED: Microviridae sp. isolate 4258-1801, complete genome	902-926	4	0.84
NC_021280_1	1.1\|366564\|25\|NC_021280\|CRISPRCasFinder	366564-366588	25	NZ_CP009118	Borreliella valaisiana Tom4006 plasmid cp26, complete sequence	25228-25252	4	0.84
NC_021280_1	1.1\|366564\|25\|NC_021280\|CRISPRCasFinder	366564-366588	25	NZ_CP034163	Escherichia albertii strain 06-3542 plasmid p06-3542, complete sequence	88828-88852	4	0.84
NC_021280_1	1.1\|366564\|25\|NC_021280\|CRISPRCasFinder	366564-366588	25	NZ_CP024444	Moraxella osloensis strain NP7 plasmid pNP7-1, complete sequence	7730-7754	5	0.8
NC_021280_1	1.1\|366564\|25\|NC_021280\|CRISPRCasFinder	366564-366588	25	NZ_CP040258	Moraxella osloensis strain MOXF1 plasmid pMOXF1, complete sequence	7048-7072	5	0.8
NC_021280_1	1.1\|366564\|25\|NC_021280\|CRISPRCasFinder	366564-366588	25	NZ_CP013780	Piscirickettsia salmonis strain PM51819A plasmid p2PS5, complete sequence	47666-47690	5	0.8

1. spacer 1.1|366564|25|NC_021280|CRISPRCasFinder matches to NC_006872 (Clostridium perfringens plasmid pBCNF5603 DNA, complete sequence) position: , mismatch: 2, identity: 0.92

agattaattaatttactattttaaa	CRISPR spacer
agattaatcaattgactattttaaa	Protospacer
********.**** ***********

2. spacer 1.1|366564|25|NC_021280|CRISPRCasFinder matches to NZ_CP013044 (Clostridium perfringens strain JP55 plasmid pJFP55J, complete sequence) position: , mismatch: 2, identity: 0.92

agattaattaatttactattttaaa	CRISPR spacer
agattaatcaattgactattttaaa	Protospacer
********.**** ***********

3. spacer 1.1|366564|25|NC_021280|CRISPRCasFinder matches to NZ_CP011154 (Bacillus cereus strain CMCC P0011 plasmid pRML05, complete sequence) position: , mismatch: 3, identity: 0.88

agattaattaatttactattttaaa	CRISPR spacer
atattaattgatttactattataaa	Protospacer
* *******.********** ****

4. spacer 1.1|366564|25|NC_021280|CRISPRCasFinder matches to NZ_CP011152 (Bacillus cereus strain CMCC P0021 plasmid pRML04, complete sequence) position: , mismatch: 3, identity: 0.88

agattaattaatttactattttaaa	CRISPR spacer
atattaattgatttactattataaa	Protospacer
* *******.********** ****

5. spacer 1.1|366564|25|NC_021280|CRISPRCasFinder matches to MG945592 (UNVERIFIED: Microviridae sp. isolate 4258-1801, complete genome) position: , mismatch: 4, identity: 0.84

agattaattaatttactattttaaa	CRISPR spacer
tccttaattaatttactatttttaa	Protospacer
   ******************* **

6. spacer 1.1|366564|25|NC_021280|CRISPRCasFinder matches to NZ_CP009118 (Borreliella valaisiana Tom4006 plasmid cp26, complete sequence) position: , mismatch: 4, identity: 0.84

agattaattaatttactattttaaa	CRISPR spacer
ttattaattaatttactatttataa	Protospacer
  *******************  **

7. spacer 1.1|366564|25|NC_021280|CRISPRCasFinder matches to NZ_CP034163 (Escherichia albertii strain 06-3542 plasmid p06-3542, complete sequence) position: , mismatch: 4, identity: 0.84

agattaattaatttactattttaaa	CRISPR spacer
gcactaattaatttattattttaaa	Protospacer
. *.***********.*********

8. spacer 1.1|366564|25|NC_021280|CRISPRCasFinder matches to NZ_CP024444 (Moraxella osloensis strain NP7 plasmid pNP7-1, complete sequence) position: , mismatch: 5, identity: 0.8

agattaattaatttactattttaaa	CRISPR spacer
tatttaatttatttactattttaat	Protospacer
 . ****** **************

9. spacer 1.1|366564|25|NC_021280|CRISPRCasFinder matches to NZ_CP040258 (Moraxella osloensis strain MOXF1 plasmid pMOXF1, complete sequence) position: , mismatch: 5, identity: 0.8

agattaattaatttactattttaaa	CRISPR spacer
tatttaatttatttactattttaat	Protospacer
 . ****** **************

10. spacer 1.1|366564|25|NC_021280|CRISPRCasFinder matches to NZ_CP013780 (Piscirickettsia salmonis strain PM51819A plasmid p2PS5, complete sequence) position: , mismatch: 5, identity: 0.8

agattaattaatttactattttaaa	CRISPR spacer
taattaattaatatactattttatt	Protospacer
 .********** **********

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

398673 : 407504

Bacillus_phage(25.0%)

transposase

The bacterium proteins that are colored denote the protein is present at specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin' and 'tRNA')

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_016338773.1\|398673_399543_+	thymidylate synthase	A0A0N9SH48	Staphylococcus_phage	2.8e-89	53.2
WP_016338774.1\|399542_400025_+	dihydrofolate reductase	D0VXF2	Bacillus_phage	4.4e-20	37.9
WP_016338775.1\|400025_400814_+	1-acyl-sn-glycerol-3-phosphate acyltransferase	NA	NA	NA	NA
WP_016338776.1\|400824_402135_+	thymidine phosphorylase	A0A0H3UZD4	Geobacillus_virus	5.8e-107	46.9
WP_016338777.1\|402278_403016_+	segregation/condensation protein A	A0A1B1IVW1	uncultured_Mediterranean_phage	8.8e-12	35.1
WP_016338778.1\|403031_403580_+	SMC-Scp complex subunit ScpB	A0A1B1IVT7	uncultured_Mediterranean_phage	9.2e-14	33.3
WP_016338779.1\|403581_404331_+	rRNA pseudouridine synthase	NA	NA	NA	NA
WP_016338780.1\|404346_404946_+	deoxynucleoside kinase	A0A127AVX2	Bacillus_phage	1.4e-23	33.7
WP_016338781.1\|404987_405551_-	inorganic diphosphatase	A0A2H4J8U1	uncultured_Caudovirales_phage	1.0e-23	41.0
WP_016338782.1\|405682_406384_+	hypothetical protein	NA	NA	NA	NA
WP_016338484.1\|406436_407504_-\|transposase	IS481 family transposase	A8RHK4	Spiroplasma_virus	1.1e-44	35.2

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage