CRISPRimmunity

Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Self targeting spacer number	Target MGE spacer number	Prophage number	Anti-CRISPR protein number
NZ_CP010056	Hymenobacter sp. DG25B plasmid, complete sequence	2 crisprs	NA	0	1	0	0
NZ_CP010057	Hymenobacter sp. DG25B plasmid, complete sequence	0 crisprs	NA	0	0	0	0
NZ_CP010055	Hymenobacter sp. DG25B plasmid, complete sequence	0 crisprs	DEDDh,WYL	0	0	0	0
NZ_CP010054	Hymenobacter sp. DG25B, complete genome	1 crisprs	csa3,DEDDh,cas3	0	0	1	0

1. NZ_CP010056

CRISPR-Cas detection and classification

Crispr_ID: NZ_CP010056_1

• CRISPR_ID: NZ_CP010056_1
• CRISPR_location: 6882-7135
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 4 spacers

Consensus repeat of NZ_CP010056_1

Consensus_repeat	Method
TCATCTACGTGAAAAATGGCACT	CRISPRCasFinder
CTACGTGAAAAATGGCACT	CRT

spacers of NZ_CP010056_1

>1.1|6905|21|NZ_CP010056|CRISPRCasFinder
CTGCTGCGTGAAAAATGGCAC
>1.2|6949|40|NZ_CP010056|CRISPRCasFinder
ATCTACGTGAAAAACGGCACTGTAAAGCGTGAAGGATGGC
>1.3|7012|44|NZ_CP010056|CRISPRCasFinder
TCAGGGGCGGCGTACCCCGGTTGTAAGGCTGCGGATGCGGCCGG
>1.4|7079|34|NZ_CP010056|CRISPRCasFinder
GTCGGGCTGGCTGGGCCCGGTAACTTAGGCTTGT
>1.5|6905|25|NZ_CP010056|CRT
CTGCTGCGTGAAAAATGGCACTCTC
>1.6|6949|44|NZ_CP010056|CRT
ATCTACGTGAAAAACGGCACTGTAAAGCGTGAAGGATGGCACTC
>1.7|7012|48|NZ_CP010056|CRT
TCAGGGGCGGCGTACCCCGGTTGTAAGGCTGCGGATGCGGCCGGTCAT
>1.8|7079|38|NZ_CP010056|CRT
GTCGGGCTGGCTGGGCCCGGTAACTTAGGCTTGTTGAT

CRISPR arrays and Neighbor proteins around NZ_CP010056_1

CRISPR arrays

The CRISPR arrays of NZ_CP010056_1

>merge|NZ_CP010056|1|6882-7135|CRISPRCasFinder,CRT
TTTTCTGCGTGAAAAACGGCACTCTGCTGCGTGAAAAATGGCACTCTCCTACGTGAAGGATGGCACCATCTACGTGAAAAACGGCACTGTAAAGCGTGAAGGATGGCACTCCTACGTGAAAAATGGCACTTCAGGGGCGGCGTACCCCGGTTGTAAGGCTGCGGATGCGGCCGGTCATCTACGTGAAAAATGGCACTGTCGGGCTGGCTGGGCCCGGTAACTTAGGCTTGTTGATCTACGTGAAAAATGGCACT

>NZ_CP010056|1|1|6882-7135|CRISPRCasFinder
TTTTCTGCGTGAAAAACGGCACT	CTGCTGCGTGAAAAATGGCAC
TCTCCTACGTGAAGGATGGCACC	ATCTACGTGAAAAACGGCACTGTAAAGCGTGAAGGATGGC
ACTCCTACGTGAAAAATGGCACT	TCAGGGGCGGCGTACCCCGGTTGTAAGGCTGCGGATGCGGCCGG
TCATCTACGTGAAAAATGGCACT	GTCGGGCTGGCTGGGCCCGGTAACTTAGGCTTGT
TGATCTACGTGAAAAATGGCACT

>NZ_CP010056|1|1|6886-7135|CRT
CTGCGTGAAAAACGGCACT	CTGCTGCGTGAAAAATGGCACTCTC
CTACGTGAAGGATGGCACC	ATCTACGTGAAAAACGGCACTGTAAAGCGTGAAGGATGGCACTC
CTACGTGAAAAATGGCACT	TCAGGGGCGGCGTACCCCGGTTGTAAGGCTGCGGATGCGGCCGGTCAT
CTACGTGAAAAATGGCACT	GTCGGGCTGGCTGGGCCCGGTAACTTAGGCTTGTTGAT
CTACGTGAAAAATGGCACT

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
NZ_CP010056.1|WP_044517964.1|10646_12059_-|chloride-channel-protein	unknown	unknown	gnl|CDD|238506
NZ_CP010056.1|WP_044518146.1|13556_14015_+|DUF305-domain-containing-protein	unknown	unknown	gnl|CDD|367619
NZ_CP010056.1|WP_082017242.1|7149_8256_+|replication-initiation-protein	unknown	unknown	gnl|CDD|376444
NZ_CP010056.1|WP_044517980.1|20594_21944_+|HAMP-domain-containing-histidine-kinase	unknown	unknown	gnl|CDD|223715
NZ_CP010056.1|WP_082017243.1|9009_10350_+|hypothetical-protein	unknown	unknown	gnl|CDD|223104
NZ_CP010056.1|WP_044517966.1|12083_13352_-|sodium:proton-antiporter	unknown	unknown	gnl|CDD|223104
NZ_CP010056.1|WP_048826535.1|2051_2366_+|LacI-family-transcriptional-regulator	unknown	unknown	gnl|CDD|224525
NZ_CP010056.1|WP_044517978.1|19871_20543_+|response-regulator-transcription-factor	unknown	unknown	gnl|CDD|223816
NZ_CP010056.1|WP_044517957.1|5831_6284_+|hypothetical-protein	unknown	unknown	gnl|CDD|237030
NZ_CP010056.1|WP_082017244.1|14029_15571_-|DUF389-domain-containing-protein	unknown	unknown	gnl|CDD|377221
NZ_CP010056.1|WP_044517954.1|5034_5832_+|ParA-family-protein	unknown	unknown	gnl|CDD|224113
NZ_CP010056.1|WP_082017241.1|2391_3111_+|LacI-family-transcriptional-regulator	unknown	unknown	gnl|CDD|380491
NZ_CP010056.1|WP_044517972.1|16247_16922_+|SOS-response-associated-peptidase	unknown	unknown	gnl|CDD|376837
NZ_CP010056.1|WP_044517952.1|1577_1787_+|heavy-metal-associated-domain-containing-protein	unknown	unknown	gnl|CDD|225328
NZ_CP010056.1|WP_044517975.1|17042_19526_-|glycoside-hydrolase-family-2-protein	unknown	unknown	gnl|CDD|225789

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
NZ_CP010056.1|WP_044517964.1|10646_12059_-|chloride-channel-protein	gnl|CDD|238506	cd01034, EriC_like, ClC chloride channel family. These protein sequences, closely related to the ClC Eric family, are putative halogen ion (Cl-, Br- and I-) transport proteins found in eubacteria. They belong to the ClC superfamily of chloride ion channels, which share a unique double-barreled architecture and voltage-dependent gating mechanism. This superfamily lacks any structural or sequence similarity to other known ion channels and exhibit unique properties of ion permeation and gating. The voltage-dependent gating is conferred by the permeating anion itself, acting as the gating charge.	2.73843e-97
NZ_CP010056.1|WP_044518146.1|13556_14015_+|DUF305-domain-containing-protein	gnl|CDD|367619	pfam03713, DUF305, Domain of unknown function (DUF305). Domain found in small family of bacterial secreted proteins with no known function. Also found in Paramecium bursaria chlorella virus 1. This domain is short and found in one or two copies. The domain has a conserved HH motif that may be functionally important. This domain belongs to the ferritin superfamily. It contains two sequence similar repeats each of which is composed of two alpha helices.	1.3509e-10
NZ_CP010056.1|WP_082017242.1|7149_8256_+|replication-initiation-protein	gnl|CDD|376444	pfam01051, Rep_3, Initiator Replication protein. This protein is an initiator of plasmid replication. RepB possesses nicking-closing (topoisomerase I) like activity. It is also able to perform a strand transfer reaction on ssDNA that contains its target. This family also includes RepA which is an E.coli protein involved in plasmid replication. The RepA protein binds to DNA repeats that flank the repA gene.	7.07854e-42
NZ_CP010056.1|WP_044517980.1|20594_21944_+|HAMP-domain-containing-histidine-kinase	gnl|CDD|223715	COG0642, BaeS, Signal transduction histidine kinase [Signal transduction mechanisms].	2.50968e-52
NZ_CP010056.1|WP_082017243.1|9009_10350_+|hypothetical-protein	gnl|CDD|223104	COG0025, NhaP, NhaP-type Na+/H+ and K+/H+ antiporters [Inorganic ion transport and metabolism].	5.22458e-26
NZ_CP010056.1|WP_044517966.1|12083_13352_-|sodium:proton-antiporter	gnl|CDD|223104	COG0025, NhaP, NhaP-type Na+/H+ and K+/H+ antiporters [Inorganic ion transport and metabolism].	2.14896e-60
NZ_CP010056.1|WP_048826535.1|2051_2366_+|LacI-family-transcriptional-regulator	gnl|CDD|224525	COG1609, PurR, Transcriptional regulators [Transcription].	3.47573e-34
NZ_CP010056.1|WP_044517978.1|19871_20543_+|response-regulator-transcription-factor	gnl|CDD|223816	COG0745, OmpR, Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain [Signal transduction mechanisms / Transcription].	5.73849e-79
NZ_CP010056.1|WP_044517957.1|5831_6284_+|hypothetical-protein	gnl|CDD|237030	PRK12270, kgd, multifunctional oxoglutarate decarboxylase/oxoglutarate dehydrogenase thiamine pyrophosphate-binding subunit/dihydrolipoyllysine-residue succinyltransferase subunit.	1.44368e-07
NZ_CP010056.1|WP_082017244.1|14029_15571_-|DUF389-domain-containing-protein	gnl|CDD|377221	pfam04087, DUF389, Domain of unknown function (DUF389). Family of hypothetical bacterial proteins with an undetermined function.	5.97521e-50
NZ_CP010056.1|WP_044517954.1|5034_5832_+|ParA-family-protein	gnl|CDD|224113	COG1192, Soj, ATPases involved in chromosome partitioning [Cell division and chromosome partitioning].	1.97482e-27
NZ_CP010056.1|WP_082017241.1|2391_3111_+|LacI-family-transcriptional-regulator	gnl|CDD|380491	cd06267, PBP1_LacI_sugar_binding-like, ligand binding domain of the LacI transcriptional regulator family belonging to the type 1 periplasmic-binding fold protein superfamily. Ligand binding domain of the LacI transcriptional regulator family belonging to the type 1 periplasmic-binding fold protein superfamily. In most cases, ligands are monosaccharide including lactose, ribose, fructose, xylose, arabinose, galactose/glucose, and other sugars. The LacI family of proteins consists of transcriptional regulators related to the lac repressor. In this case, the domain sugar binding changes the DNA binding activity of the repressor domain.	2.91793e-73
NZ_CP010056.1|WP_044517972.1|16247_16922_+|SOS-response-associated-peptidase	gnl|CDD|376837	pfam02586, SRAP, SOS response associated peptidase (SRAP). The SRAP family functions as a DNA-associated autoproteolytic switch that recruits diverse repair enzymes onto DNA damage. We propose that the human protein Q96FZ2:UniProtKB, the eukaryotic member of the SRAP family, which has been recently shown to bind specifically to DNA with 5-hydroxymethylcytosine, 5-formylcytosine and 5-carboxycytosine, is a sensor for these oxidized bases generated by the TET (tetrahedral aminopeptidase of the M42 family) enzymes from methylcytosine. Hence, its autoproteolytic activity might help it act as a switch that recruits DNA repair enzymes to remove these oxidized methylcytosine species as part of the DNA demethylation pathway downstream of the TET enzymes.	2.31785e-94
NZ_CP010056.1|WP_044517952.1|1577_1787_+|heavy-metal-associated-domain-containing-protein	gnl|CDD|225328	COG2608, CopZ, Copper chaperone [Inorganic ion transport and metabolism].	9.04735e-08
NZ_CP010056.1|WP_044517975.1|17042_19526_-|glycoside-hydrolase-family-2-protein	gnl|CDD|225789	COG3250, LacZ, Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].	2.94002e-63

Protein sequences

>NZ_CP010056.1|WP_044517957.1|5831_6284_+|hypothetical-protein
MAIPIQNPKIPTRRGPIPVSEEARSQARANMGGEQVKTVLPVPAEAPAAAPAPTAAPTVAPAPAATAPAPAQKAPVAARRGRKPAPPEAVPMETERSVKIPESVWDEIRLALVLLPKGQDSPASIKAYLVAAHRVYDASLRKAGKLPPVK
>NZ_CP010056.1|WP_044517954.1|5034_5832_+|ParA-family-protein
MPVSKIISFATQKGGSGKSTLALVLAAPLAIDYGLKVAILDCDYQQSIVKTREQRDAHNLALLQEADPTASYPYDVFPIALDKIFEFIDDPAHDYDLVIIDVPGRADGDDVFNALTTCDAVIVPMVADYLDRASTAEFMGILEAIKQSAVEAHLEFQYYGLSTKRFAGQREEKEMDEYIDGLGLSRFDSFLSHRTAYKRASTLYSLLNPAYRAYAGGNKDTSDEICRVCEELIERLGLPNRQAQAEAAPQVSTAVIPSTSTIETE
>NZ_CP010056.1|WP_082017241.1|2391_3111_+|LacI-family-transcriptional-regulator
MEALLAAQVEGILISVSATTFSETHHFEQVRQQGTPLVFFDRMPELPQSMAVILDDFQGAYQAVRHLIEQGCTRIAHLAGPQHLNTSRNRFLGYKAALEAHGLPFDEQWVYALPALTHDAGRLGMQHLLAQIPPLDAVFAAYAIPTVGALEVLREKSKRVPQDIALACFSNEPFTTMIQPQLTVVDQRAEQMGETAVRLFLQLLKRGPSYVPPHLVLKPELIIRESSLHRSQEARPLKS
>NZ_CP010056.1|WP_048826535.1|2051_2366_+|LacI-family-transcriptional-regulator
MAASRQKTSIRDIAQLLGLSTSTVSRGLADHKDISEATKERIRQKAQELNYRPNQLAAALRKGHSKTLGVIVPHINGYFFPAVMNGIEKIATQEGFTLMMCPVE
>NZ_CP010056.1|WP_044517952.1|1577_1787_+|heavy-metal-associated-domain-containing-protein
MKTLQFKTNINCGGCIKAVTPALNQEAGAGNWQVDTANPDKILTVTTDQLTAEQIVAVVENAGFEIQAA
>NZ_CP010056.1|WP_082017242.1|7149_8256_+|replication-initiation-protein
MKGGIFSRSSVYLRVIPFYPFAYLLVAVTPAPLYPRAYQANALVRAPLKLTHVEARIFALALGCIHQDQTELPGISIPLSRVMTQKKGGSVYETIRDACKSLMSKVVSIESQTGNKKRFTAYSIISYIDLNEGTGYLTGNFAPEIKPFLLQLAEQYTHVEIETLLTLKSAHAHRLYWLLKSWDDVGLWEVDFDTLRKQVLGDDNDVTYTLFYDFKRYVLEPALRELHSLGWEVTYEPVKEGKKVKGVRFSIPKPQVRIQEGGLEERPTRTAGKARLAEKQMSLPLSGELLTLQQRIVTRLQKLRMTAAQIQHVLEFVGDDEVKIARLMKVTHPLLRDFEAGNKVFDNLGGAAINILKSEFPGLYATLK
>NZ_CP010056.1|WP_082017243.1|9009_10350_+|hypothetical-protein
MFSATGMRWVNGTQPRPVNYWPMTTPLLIILIGFLIFLGHYLAQFFQRTRVPDVVGLLLIGMLLGPVFHLIDPASFGKAGRVFTNLVLVFILFESGLEVRFEQLRSALRGTMSLTTFNFIVTTLVVAALAQWLTDLDFLSSLILGTILGGTSSAVVTTLARQARILPQTATTLVMESAFSDIYTLAIPISLISFYSGATFNVASLLGQLLSSLVVAVLVGVGCGYLWLLLLNRVPTLLKTRFSTPAFAFILYGFIELMQFSGPIAVLFFSITLGNLPLLRRGWLSAILPARAVELTEVEKDFFAEIVFLLRTFFFVYVGMSVVLDNFYLIALGTGMAVLLFLVRIPVVMAAAERNTPQFDMAMMSIMIPKGLGAAVLATLPAQRGLPGGEIIQTITFAVIVATTVLCTGLFFLLEKRHILGFYQLFFRSPRPIDAEAAPALSKRGN
>NZ_CP010056.1|WP_044517964.1|10646_12059_-|chloride-channel-protein
MSDSPSRYARILDWLDQHVIRPFYTDRVRRLILQSFPFWIASILTGMVAVGYEKVFVWAEEVSFSWLGRTPWLAFVLTPLAFGASWLLVRQWAPAARGSGIPQVMAGIELSNPGQHHRTGYLLSLRVAIVKVLSSVVLLLGGGVIGREGPTIQISAAIFRAINKLQPAGWPQLSRQIALVTGGAAGLAAAFNTPLGGIVFVVEELTQMHMARFRMAVFTAVIIAGLTAQTFLGPYLYLGFPKIVPAAGRVQVLVLVVALGCGLAGAAFAKTLLWLGRYRQRFTSLAAQAGWVVGCGMLLGGLAYLVGSEGVGTGKPIINRLLFQNNGQTPWYLFPVRFAGMALSYSSGGAGGIFATSLSAGAILGDGICQVFNVAVADRNLLILVAMVSFLTGVVRSPFTAAILVLEMTDRHSAIFQLLISGLLAQGVASLVDPHSLYEHLKAGFIRETGDVDRATQLPPRPHNGSQTAD
>NZ_CP010056.1|WP_044517966.1|12083_13352_-|sodium:proton-antiporter
MELYNTLALLIVVAAVFGYINHRFLKLPGTIGLMVLALVSSLLAIGLGKLGVEWVLTAGQLVRGIDFHTVLMQVMLSFLLFAGSLHVDVRALGREGLAVGTMATVGTLLSTALVGTAFYYLLPVFGLSTDFIYCLLFGALISPTDPIAVLGILKEARIDKRLEIRIVGESLFNDGIAVVVFISLYQIALYGAEKATPGIIGHLFLQEAVGGILLGAALGYATYWALRSIDNYQVEVMITLALVMGGTALATALHTSGPLAMVVAGLIVGDQGRELGMSDETREYLDKFWELLDEILNAILFVLIGLEMLVLHISHTTLLVGLVSIVLVLLARWVSVGVPLVVLQRFYPFDRQTLRVLTWGGLRGGISVALALSLPDSMPRDLLVGVTYVVVIFSIIVQGLSIGPLVKRLGLSTGPAEDPAAH
>NZ_CP010056.1|WP_044518146.1|13556_14015_+|DUF305-domain-containing-protein
MSPHPYRKFALMLVVSFVVMYAVMFLNVYQPNHIYLSLTRTYMSLLMVAAMALVMLGLMRHMYPDKRTNLLIAGGSVAVFVLVLTLLRAQIPVGDVQYMKAMIPHHSSAIMVSSSAELKDPEVKKLAEGIIQSQEREIAEMKAAIARLEGQQ
>NZ_CP010056.1|WP_082017244.1|14029_15571_-|DUF389-domain-containing-protein
MVSEYGGEDTAGALRGFAQPTSGAAPAHRRGGAGPATLRGGSHFRGYCASHCVVKSRINPMPIPFLHWLRSRFDLANDMADPAEIVADVEAGIAFKGTNLWVLLFAILVASVGLNVNSTAVIIGAMLISPLMGPIVGVGFGAATANLDLVRRGLKNLSIAAGLSVLVSALYFSFTPLTEAGSELLARTTPTTWDVAIALFGGAAGAIGFTRREVSNVVPGVSIATALMPPLCTAGYGLATAHWSFLAGAMYLFCINVAFISLAMFLVARLLPLPHHAFVNRQQARRAKLLFWGVAVLTAVPSVYLAARIVRRTTFEHNAQRFVDAELNLPGAYVVTRRIEADKGTINVLLTGRMLTLTQLRTVRARLQDYHLRRAQLTVRQGLAQLDSADARALRTSLLEDLRSRNEQTLAGYEARLAQLQQLLTQQATYAARLPAPNVLLREVQAEHPTVRQLALSQLTHSATDSLRADTTVVVSIRATRPLPAAEVQRLTEWLTVRAGTRLPVRLLLDGAL
>NZ_CP010056.1|WP_044517972.1|16247_16922_+|SOS-response-associated-peptidase
MCGRYTFITPPGLIEERFEATMPDFPATYNAAPSQQLPVITNTAPQQVQLVQWGLVPAWVKDRTSGPQPINARAETLAEKPSFRQLLQRRRCLVLADSFYEWQEKETHGLPAKTPHRILLRSGEPYAFAGLWDEWIDRATGEVLPTFTIVTTAPNALMQPLHHRMPVILQGRAAERAWLNDAVSAAEHQQLLAPYPAELMQEYVISKLVNSPAHNSPEVLEPVA
>NZ_CP010056.1|WP_044517975.1|17042_19526_-|glycoside-hydrolase-family-2-protein
MTEFIARIAARSLAAGLLLTACLTAQAQTRQEYLLTTNWKFAKGDQPNAALPDFPDAKWQTVSVPHDWAIYGPFSSSNDLQTVKIEQNNEKEAKTKAGRTGGLPFIGTGWYRRRLEVPGFAPGKQAILLFDGAMSDAHVFVNGQEAGAWPYGYNSFAVDITPYLHAKGANTLAVRLQNKPEASRWYPGAGLYRNVHLIVTQDVHIPVWGTYLTTPVINADFARVKLRTQVETAGKAFQPLRLRTEIRDAAGKVVATTSTMLAATDGQEFEQNMVVPKPQLWSPETPVLYTASSKLYAGEVLKDEYQTPFGIRSFTFDPAKGFSLNGQPRKFKGVCNHHDLGPLGAAVNTAALRHQLTLLKDLGCDAIRTSHNMPAPELVQLCDEMGFMLMVESFDEWKTPKVKNGYSLLFDQWAEKDLVNMVHRDRNHPSVIMWSIGNEVPDQWAPGGTKLAKRLQDIVHREDPTRPVTAGMDQFDAAVGNGFAALLDIPGFNYKPSRYPEAYTKLPQGMLLGSETASTVSSRGVYKFPVVQAKDKQYPDNQSSSYDLEASNWSQIPDEEFAAQDDLPYTLGEFVWTGFDYLGEPWPYDEKWPSHSSYFGIIDLAGIPKDRYYLYRARWNTTQPTLHVLPHWSWLGREGQTTPVFVYTSYPSAELFVNGVSQGRQTKYKSDKPQTRYRLMWNEVKYQPGKIKVVAYDAQGKPVAEEEVRTAGKPHHIRLVADRDTLTADGQDLAYVTARVEDAQGNLCPEATNQLQFKVSGAGSFRAVGNGDPTNLQPFHLPRMKAFQGQLVAIVQAAAIAGEVQLKAISPSLKGTTLRLHTTKTAR
>NZ_CP010056.1|WP_044517978.1|19871_20543_+|response-regulator-transcription-factor
MKILLVEDEVKLSSFVRKGLEGEGYWVDTAFDGQLGLSMALRGEYDLVILDVSLPQLNGFEVCRRLRAERPQVAILLLTALGGIAHKTEGYDAGADDYLVKPFEFQELLLRVRALYRRYHDAGPGRTLSVADLTLHLTTKRVERSGQPIALTAREYALLEYLLLNQGHVVSRVDIAEKVWELKFDTNTNIIEVYITYLRRKIDKDFSPKLIHTVVGMGYVLRA
>NZ_CP010056.1|WP_044517980.1|20594_21944_+|HAMP-domain-containing-histidine-kinase
MTIRARLTLFFAGLMLALVAAVSCGVYFFERNSQRGQFEGLLLRKAEATAKVYVRRHEHLEPTDAILLLTQQNQYEAIYDDDNNLLYASRPNSGLAITPGFLARTRLAGRLFFSPGPAVGVALYTKSGNEGLVVVITAEDKYGQEGLQRLRNNLLISNILGLTFVVGLANLFAGRALKPVQRLSLEIQALSSRTLDQRLSTGNGRDELARLAAQFNALLARLADTVAQNRNFVWYASHELRTPLANLLGTLDISRAYDERPQDMRATLSSATEEVRRLISLTNDLLLFAELSQEKPAHDLPLTPVLLTEPLLDAVQAVQRRYPANTIELHLPEAVEDCQARAHAELLTLALVNLLDNACKYSPAGSAVHVSLARSGGGCAFTVRDTGIGIAPEALPHIFEPMYRAEDVRTRFSGNGVGLSLVQRVAELHGGGVAVQSTVGKGTEFRLWL

Crispr_ID: NZ_CP010056_2

• CRISPR_ID: NZ_CP010056_2
• CRISPR_location: 101589-101666
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 1 spacers

Consensus repeat of NZ_CP010056_2

Consensus_repeat	Method
TCGGCTTATAACATTTATTAAACG	CRISPRCasFinder

spacers of NZ_CP010056_2

>2.1|101613|30|NZ_CP010056|CRISPRCasFinder
GAGGTTCAACAGGTGTTATACCTCAGCCTA

CRISPR arrays and Neighbor proteins around NZ_CP010056_2

CRISPR arrays

The CRISPR arrays of NZ_CP010056_2

>merge|NZ_CP010056|2|101589-101666|CRISPRCasFinder
TCGGCTTATAACATTTATTAAACGGAGGTTCAACAGGTGTTATACCTCAGCCTAATGGCTTATAACATTTATTAAACG

>NZ_CP010056|2|2|101589-101666|CRISPRCasFinder
TCGGCTTATAACATTTATTAAACG	GAGGTTCAACAGGTGTTATACCTCAGCCTA
ATGGCTTATAACATTTATTAAACG

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
NZ_CP010056.1|WP_044518092.1|122058_123384_+|sugar-porter-family-MFS-transporter	unknown	unknown	gnl|CDD|340917
NZ_CP010056.1|WP_156126560.1|94922_96074_+|hypothetical-protein	unknown	unknown	gnl|CDD|377898
NZ_CP010056.1|WP_052430620.1|106291_107047_-|copper-homeostasis-protein-CutC	unknown	unknown	gnl|CDD|225684
NZ_CP010056.1|WP_044518086.1|111007_112888_-|gamma-glutamyltransferase	unknown	unknown	gnl|CDD|223482
NZ_CP010056.1|WP_082017255.1|102295_103588_+|T9SS-type-A-sorting-domain-containing-protein	unknown	unknown	gnl|CDD|379232
NZ_CP010056.1|WP_082017254.1|101810_102320_+|Ig-like-domain-containing-protein	unknown	unknown	gnl|CDD|379073
NZ_CP010056.1|WP_048826584.1|98898_100653_+|hypothetical-protein	unknown	unknown	gnl|CDD|366935
NZ_CP010056.1|WP_048826582.1|93354_93963_+|hypothetical-protein	unknown	unknown	gnl|CDD|378065
NZ_CP010056.1|WP_048826578.1|91381_92086_-|alpha/beta-hydrolase	unknown	unknown	gnl|CDD|223669
NZ_CP010056.1|WP_156126558.1|94284_94968_+|hypothetical-protein	unknown	unknown	unknown
NZ_CP010056.1|WP_044518059.1|89447_90191_-|response-regulator-transcription-factor	unknown	unknown	gnl|CDD|225818
NZ_CP010056.1|WP_044518083.1|109759_110815_-|Fic-family-protein	unknown	unknown	gnl|CDD|225718
NZ_CP010056.1|WP_052430621.1|116891_120050_+|TonB-dependent-receptor	unknown	unknown	gnl|CDD|274948
NZ_CP010056.1|WP_044518074.1|107043_108072_-|N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase	unknown	unknown	gnl|CDD|271335
NZ_CP010056.1|WP_082017253.1|96429_98850_+|hypothetical-protein	unknown	unknown	gnl|CDD|227511
NZ_CP010056.1|WP_156126556.1|92937_93267_+|hypothetical-protein	unknown	unknown	unknown
NZ_CP010056.1|WP_048826576.1|90187_91327_-|sensor-histidine-kinase	unknown	unknown	gnl|CDD|225814
NZ_CP010056.1|WP_044518076.1|108581_109550_+|tyrosine-type-recombinase/integrase	unknown	unknown	gnl|CDD|271180
NZ_CP010056.1|WP_044518066.1|101012_101393_-|hypothetical-protein	unknown	unknown	unknown
NZ_CP010056.1|WP_044518089.1|120069_121794_+|RagB/SusD-family-nutrient-uptake-outer-membrane-protein	unknown	unknown	gnl|CDD|369630

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
NZ_CP010056.1|WP_044518092.1|122058_123384_+|sugar-porter-family-MFS-transporter	gnl|CDD|340917	cd17359, MFS_XylE_like, D-xylose-proton symporter and similar transporters of the Major Facilitator Superfamily. This subfamily includes bacterial transporters such as D-xylose-proton symporter (XylE or XylT), arabinose-proton symporter (AraE), galactose-proton symporter (GalP), major myo-inositol transporter IolT, glucose transport protein, putative metabolite transport proteins YfiG, YncC, and YwtG, and similar proteins. The symporters XylE, AraE, and GalP facilitate the uptake of D-xylose, arabinose, and galactose, respectively, across the boundary membrane with the concomitant transport of protons into the cell. IolT is involved in polyol metabolism and myo-inositol degradation into acetyl-CoA. The XylE-like subfamily belongs to the Glucose transporter -like (GLUT-like) family of the Major Facilitator Superfamily (MFS) of membrane transport proteins. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.	1.56448e-136
NZ_CP010056.1|WP_156126560.1|94922_96074_+|hypothetical-protein	gnl|CDD|377898	pfam07695, 7TMR-DISM_7TM, 7TM diverse intracellular signalling. This entry represents the transmembrane region of the 7TM-DISM (7TM Receptors with Diverse Intracellular Signalling Modules).	1.2092e-06
NZ_CP010056.1|WP_052430620.1|106291_107047_-|copper-homeostasis-protein-CutC	gnl|CDD|225684	COG3142, CutC, Uncharacterized protein involved in copper resistance [Inorganic ion transport and metabolism].	3.67009e-90
NZ_CP010056.1|WP_048826584.1|98898_100653_+|hypothetical-protein	gnl|CDD|366935	pfam02140, Gal_Lectin, Galactose binding lectin domain.	1.05425e-13
NZ_CP010056.1|WP_082017255.1|102295_103588_+|T9SS-type-A-sorting-domain-containing-protein	gnl|CDD|379232	pfam13517, VCBS, Repeat domain in Vibrio, Colwellia, Bradyrhizobium and Shewanella. This domain of about 100 residues is found in multiple (up to 35) copies in long proteins from several species of Vibrio, Colwellia, Bradyrhizobium, and Shewanella (hence the name VCBS) and in smaller copy numbers in proteins from several other bacteria. The large protein size and repeat copy numbers, species distribution, and suggested activities of several member proteins suggests a role for this domain in adhesion (TIGR).	2.98849e-09
NZ_CP010056.1|WP_044518083.1|109759_110815_-|Fic-family-protein	gnl|CDD|225718	COG3177, COG3177, Fic family protein [Function unknown].	6.51968e-43
NZ_CP010056.1|WP_048826576.1|90187_91327_-|sensor-histidine-kinase	gnl|CDD|225814	COG3275, LytS, Putative regulator of cell autolysis [Signal transduction mechanisms].	4.2557e-44
NZ_CP010056.1|WP_048826582.1|93354_93963_+|hypothetical-protein	gnl|CDD|378065	pfam08843, AbiEii, Nucleotidyl transferase AbiEii toxin, Type IV TA system. This family was recently identified as belonging to the nucleotidyltransferase superfamily. AbiEii is the cognate toxin of the type IV toxin-antitoxin 'innate immunity' bacterial abortive infection (Abi) system that protects bacteria from the spread of a phage infection. The Abi system is activated upon infection with phage to abort the cell thus preventing the spread of phage through viral replication. There are some 20 or more Abis, and they are predominantly plasmid-encoded lactococcal systems. TA, toxin-antitoxin, systems on plasmids function by killing cells that lose the plasmid upon division. AbiE phage resistance systems function as novel Type IV TAs and are widespread in bacteria and archaea. The cognate antitoxin is pfam13338.	0.00239494
NZ_CP010056.1|WP_048826578.1|91381_92086_-|alpha/beta-hydrolase	gnl|CDD|223669	COG0596, MhpC, Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily) [General function prediction only].	2.79643e-08
NZ_CP010056.1|WP_044518059.1|89447_90191_-|response-regulator-transcription-factor	gnl|CDD|225818	COG3279, LytT, Response regulator of the LytR/AlgR family [Transcription / Signal transduction mechanisms].	3.50802e-51
NZ_CP010056.1|WP_044518089.1|120069_121794_+|RagB/SusD-family-nutrient-uptake-outer-membrane-protein	gnl|CDD|369630	pfam07980, SusD_RagB, SusD family. This domain is found in bacterial cell surface proteins such SusD and SusD-like proteins, as as well RagB, outer membrane surface receptor antigen. Bacteroidetes, one of the two dominant bacterial phyla in the human gut, are Gram-negative saccharolytic microorganisms that utilize a diverse array of glycans. Hence, they express starch-utilization system (Sus) for glycan uptake. SusD has 551 amino acids, and is almost entirely alpha-helical, with 22 alpha-helices, eight of which form 4 tetra-trico peptide repeats (TPRs: helix-turn-helix motifs involved in protein-protein interactions). The four TPRs pack together to create a right-handed super-helix. This is predicted to mediate the formation of SusD and SusC porin complex at the cell surface. The interaction between SusC and TPR1/TPR2 region of SusD is predicted to be of functional importance since it allows SusD to be in position for oligosaccharide capture from other Sus lipoproteins and delivery of these glycans to the SusC porin. The non-TPR containing portion of SusD is where starch binding occurs. The binding site is a shallow surface cavity located on top of TPR1. SusD homologs such as SusD-like proteins have a critical role in carbohydrate acquisition. Both SusD and its homologs, contain about 15-20 residues at the N-terminus that might be a flexible linker region, anchoring the protein to the membrane and the glycan-binding domain. Other homologs to SusD have been examined in Porphyromonas gingivalis such as RagB, an immunodominant outer-membrane surface receptor antigen. Structural characterization of RagB shows substantial similarity with Bacteroides thetaiotaomicron SusD (i.e alpha-helices and TPR regions). Based on this structural similarity, functional studies suggest that, RagB binding of glycans occurs at pockets on the molecular surface that are distinct from those of SusD.	1.71207e-41
NZ_CP010056.1|WP_052430621.1|116891_120050_+|TonB-dependent-receptor	gnl|CDD|274948	TIGR04056, OMP_RagA_SusC, TonB-linked outer membrane protein, SusC/RagA family. This model describes a distinctive clade among the TonB-linked outer membrane proteins (OMP). Members of this family are restricted to the Bacteriodetes lineage (except for Gemmatimonas aurantiaca T-27 from the novel phylum Gemmatimonadetes) and occur in high copy numbers, with over 100 members from Bacteroides thetaiotaomicron VPI-5482 alone. Published descriptions of members of this family are available for RagA from Porphyromonas gingivalis, SusC from Bacteroides thetaiotaomicron, and OmpW from Bacteroides caccae. Members form pairs with members of the SusD/RagB family (pfam07980). Transporter complexes including these outer membrane proteins are likely to import large degradation products of proteins (e.g. RagA) or carbohydrates (e.g. SusC) as nutrients, rather than siderophores. [Transport and binding proteins, Unknown substrate].	0
NZ_CP010056.1|WP_082017253.1|96429_98850_+|hypothetical-protein	gnl|CDD|227511	COG5184, ATS1, Alpha-tubulin suppressor and related RCC1 domain-containing proteins [Cell division and chromosome partitioning / Cytoskeleton].	6.74304e-28
NZ_CP010056.1|WP_044518074.1|107043_108072_-|N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase	gnl|CDD|271335	cd04513, Glycosylasparaginase, Glycosylasparaginase and similar proteins. Glycosylasparaginase catalyzes the hydrolysis of the glycosylamide bond of asparagine-linked glycoproteins. This enzyme is an amidase located inside lysosomes. Mutation of this gene in humans causes a genetic disorder known as aspartylglycosaminuria (AGU). The glycosylasparaginase precursor undergoes autoproteolysis through an N-O or N-S acyl rearrangement of the peptide bond, which leads to the cleavage of a peptide bond between an Asp and a Thr. This proteolysis step generates an exposed N-terminal catalytic threonine and activates the enzyme.	2.92242e-131
NZ_CP010056.1|WP_082017254.1|101810_102320_+|Ig-like-domain-containing-protein	gnl|CDD|379073	pfam13205, Big_5, Bacterial Ig-like domain.	8.82879e-12
NZ_CP010056.1|WP_044518086.1|111007_112888_-|gamma-glutamyltransferase	gnl|CDD|223482	COG0405, Ggt, Gamma-glutamyltransferase [Amino acid transport and metabolism].	4.81885e-122
NZ_CP010056.1|WP_044518076.1|108581_109550_+|tyrosine-type-recombinase/integrase	gnl|CDD|271180	cd00799, INT_Cre_C, C-terminal catalytic domain of Cre recombinase (also called integrase). Cre-like recombinases are tyrosine based site specific recombinases. They belong to the superfamily of DNA breaking-rejoining enzymes, which share the same fold in their catalytic domain and the overall reaction mechanism. The bacteriophage P1 Cre recombinase maintains the circular phage replicon in a monomeric state by catalyzing a site-specific recombination between two loxP sites. The catalytic core domain of Cre recombinase is linked to a more divergent helical N-terminal domain, which interacts primarily with the DNA major groove proximal to the crossover region.	5.19109e-55

Protein sequences

>NZ_CP010056.1|WP_044518066.1|101012_101393_-|hypothetical-protein
MIRSIRSTGLLATLFLTLSFAACEKKQTPAPAPPAPIKFDVIVRDDYEPNKYALDNIKILDATYSGTTAHLKLQGNLKNGSLLTVEFYQPEGTAEANKTTGVRVHSSLWGRAWPRARAAMSRRTAR
>NZ_CP010056.1|WP_048826584.1|98898_100653_+|hypothetical-protein
MKLPGNAWTSLAAGYYHAAGEQSCHAVWAWGDNSNGQIGTGKGYIDANIYQFPELVIGPGPGLIRFTPASAAVGATVVATGTGLSGLTALSVNGTDALASVTNNSDNSFTFVVPAGATATGTTSVDAGCGSASSTAFTVLPCLTPNLQSPTLTLAGSGQGTVGAGAFDAGSTASCGAITFEVQKLRTGTVYGTMAEPGLLSLTAPAGAVFTSVDFASFGNPTGSEGNYTLGSCNAPNSLSRVQEALLNRGGTVNISASSGFFNGDPCNGTSKQLAVRATYTYPVGSASSSLEYACADLGSQPVQLTLTDAQGVSMTRPVSVTVLSPPTATITGLNPVSATPGTTVMASGSNLTGATALTVNGASAAISNLTATGFTFIVPAQAASSGNLILSLPCTQLLTQPFSTTPLPVELVQFTATLAGPEAVRLTWMTASEKNSAYFEVEASADGHTYRRVGQVAGHGNTTHQQEYQLVDKGIRQYATGLVYYRLRQVDQDGTATLSPVRTVKVAPTRELALFPNPTTGSATLTGVQPGALVTVLDAVGRPVFTLHASAAGTAALVLPPGIAWGLYVVRVGPQALPLIVSQ
>NZ_CP010056.1|WP_082017253.1|96429_98850_+|hypothetical-protein
MLPFYPLHWTAPGRFARLALALLLAWIPLLAWAGTPTLRLEATVTTGDVTAFVDQVEIINAVTGAVVAGAVPNAGFETFTSLDNGSYGYQPTGATWNFAPRSGITSDGGALGNPTASPDGTHAAFLQPASGGTSLSQTLPELAAGYYQVRVQLAQRNFGTADQGVRLLVDGRDLGNSVPANDGGYHSFTSAVFAVGAELRFEGGGSVYLPGFDVTAFLDQLELVDAATGAAVPGMIANPGFETTGSLGSDLYGYRPTGTGWTFADASGIATNGSAFSSPTAPDGTHVGFIQSDNFNVGFRQILGGALAGSYQLRLKAAQRVMGDEPANQQVLVWLNNQLLATLLPSSNGTFQDFTVAFTVATPPVPVISSFTPGIGGPGTSLSLTGANLSGTRAITFPGGVVVNSGFRMSTDGTKITDVVVPAGAQSGPLTATLSGGQRAVSPQDFTIVTLPTLAQVSPYLGTPGTVLTLTGTNLTGTTSINFTGSAGVKTVTSGFTVNAAGTQITGIVVPTGAQSGPITATTATSGTSLVGPALFFRARTVAAGYQHTVAVRADGTLWAWGWNHGAQLGDGTTTNRNAPTQIGTDNNWATVSAGSGTAAIRTDGTLWAWGAGLVGDGTGLVRRYVPVQIGTATNWATVDIGEDYTVALRADGTLWIWGNGPSGYFDVPTQVGTETNWAAVDAGLYHAVGVRTDGTLWAWGFNQYGQLGTGNASNAINRGPTQVGTGTNWVSASAGGLHTMAIQANGTLWGWGRNEYNQLGTPALFDRPSPIQIGYGGSTRKTQWANVAAGKEFTLALQASGEVLT
>NZ_CP010056.1|WP_156126560.1|94922_96074_+|hypothetical-protein
MASGQSYGYSLNNVLMHLSVVLLLTGFVQMTRAYLPTQARLPRLDAALRYALRAYVVFTAVVGLVNISGFFLNYYTLLLDNVLVFGVVGLLLATTAVAYRLRLPLARTYLLANALPLILVVLMVGYHIVFGFDNNGNLLLPDLAIVSHALGFSAAISIRLQSVQQTLLAKVREANDLALNIQQKELRHREIVLKNQHIQTVLLEMQHRQQARDQHRQQLSADIQQQEANNQELQQQLEANQRELASTTLYVEQKSALLAELQKQIQELSKQRPDTQRDLTGLQSILQTSLYLDEDWSKFKRHFEQVHPRFFEELQVHYPALTKHEQRLASYFHIQLSTKEIAALLNIDPASVRRAKTRLYKKMAAADLAAGRTPPAAEPLVGE
>NZ_CP010056.1|WP_156126558.1|94284_94968_+|hypothetical-protein
MTPPSMGTVGVLPDRNYAWERIRTDTTLAFQAADSLRLAQDHRFWLKLTVRNPSRYTAAVQLNVLPNLDNTLFYFDEDARTWRTRRAGVAVVTDSQRVKGPLRLLLPGQSTTPVYVLIRLNQRAALPPAIRLQVKLENEAEARSRDAFFRIAWTVSMAVLGLLLLTNLPTYIRFRDRTTLYYLCTQVGAMLYITAFRAILRWCGPRRFSANWYWPLGRAMVTRSTTY
>NZ_CP010056.1|WP_048826582.1|93354_93963_+|hypothetical-protein
MNLYAATSLGIPEPRVTDDVDCIVEGAPWASFYQLEEELRALGFTNDMESGVICRWRFGQLVVDMMPTEGDILGFINPLYPEGFAHAIDYQLPDRTSIRFLAPVYFLATKLVALRDRGWEDLRLSQDLEDVVHLVDNRPQLAQEVAAADEGVRRYIQQQIREVLTHPDLLEALEWTLPAGSGYERKFEIERRLQHVAGLTSG
>NZ_CP010056.1|WP_156126556.1|92937_93267_+|hypothetical-protein
MRHGLPYVFPAHPGFQARGVLTGPSASFLQPQFGEGIAYVWPTADGEAWGAAVTPLYPGAPAAARQDPTLYELLALVDTIRLGRPREVKLAGGLLQQRLLTLPQRMHAS
>NZ_CP010056.1|WP_048826578.1|91381_92086_-|alpha/beta-hydrolase
MPRDQPGGFRGRGAGRWASSTARFLPAVRDQLLAYIKTQKLERPAIIGHSLGGFMGLWLSTTQPEAVGPLVILDSLPFLAAVQNPATTVETARPMAEGMRQQMKTGNMSVAQQRQLATGMVSDTAHQSLVARWGQASDPASVGQAMYEMYTTDLRAALGTVRQPALVLGSWAAYQPYGATLASTRAIFAAQYAQLPQHRIEMSPAGKHFLMYDDTAWFFQQTDAFLQAHYAGKK
>NZ_CP010056.1|WP_048826576.1|90187_91327_-|sensor-histidine-kinase
MSMLSAPKPSRNYWRTQVLAWTLYGIFNLVLYRAFVPPTSSTPRWVFDVMSVLIAATALGASHVLRQLIWRGQWLARPPLALLGRLLLANAVLAVGSVVMIGLVSKLALPLSVASRGPQGWAAQLGYCMNLAILLTLWAAAYVGWYYLHRSRRAEIEKWQLTAAVREAELNTLRAQLNPHFLFNGLNNIRALVSEDPERARQALAHLAELLRYVMQHSATPLVPLGQELDIVADYLALEALQLEDRLQYSLDVDPATRAVPLPPLTVQLLVENAIKHGVAGRPAGGSVALTAQLDADGWLCLTVRSPGRYYPAQAAESSGLGLRSLRERLAQAFGPAAAFALANDPAAADTVLATLRLPGSTRMAPELSSDFTILPLVR
>NZ_CP010056.1|WP_044518059.1|89447_90191_-|response-regulator-transcription-factor
MRVLLVDDSRLARAELRRLLAAFPDVEVVGEARHAEEARAQLAALRPDLLLLDIHMPGETGFELLASLEAAPPVVFTTAYDAHALRAFEVNALDYLLKPVQEARLAAALDKVRAQMPAESPDNRLQSPATAAPALLTGQDQVFVKDGERCWFVRLADVRLFEINGSYTQLYFDQHRPLIPRTLAQLEARLDPKEFFRANRQQIINLHFVAGVEPWFSHSLRLTLRGGAEVVEVSRQQSVRFRELLSL
>NZ_CP010056.1|WP_082017254.1|101810_102320_+|Ig-like-domain-containing-protein
MASIFVAYSFLLLVILKISTPYTLARTTALYLVSCLLPNLALAQAFTVSAVTPTHNTLAVPRAASVGVTFSQAVGNSASSLAGLQVFSAQRGGKLAGTSTVAGSTLTFDPLRAFKPGEQISVTLTSALKSTAGAALAKPYVYQFTAAATGGTGSYTRAPISRWVGLSLV
>NZ_CP010056.1|WP_082017255.1|102295_103588_+|T9SS-type-A-sorting-domain-containing-protein
MGRVVSGVTAADVDQDGDMDMLSVDSREGSATVRLNKGDGTFVDGSQVTVGSGPGTIAAVDVDNDGDLDVLTSNWEEGTISVRMNNGQGVFSGTQQVAVKQKPNSITTGDVDGDGDMDMLIPNWGMACTADGCPVPTVSIRLNDGKGIFSGSQNVTVLKGPTEVVAADIDSDGDLDFVVSCQEAISIRFNDGKGNYSLGKDMLLDPSVGAPTTFANITSTDVDGDGDIDLILPDGEETVGIYLNNGQGEFTLDSRITIGLVAPLCVKTCDVDGDGDQDVLCSTQFDNYIHIRLNDGHGRFSGTQKEVVWKTVCSIANADLDGDGDMDLLTATFEKNIISVHYNNGTGAVLAAASAQQPAGVSLFPNPMQGALRVQGLASQATLIVRNAVGQQVYAAQANQKGEAILSLEAAPAGVYFVQAGNQVYRFLKE
>NZ_CP010056.1|WP_052430620.1|106291_107047_-|copper-homeostasis-protein-CutC
MNTRRLEICAGSVQSALAAQAGGADRIELCQNLEQGGIKPSYGTIQRVLSQLSIPVFVLIRPRPGHFCYDAEEQAIMVADIRQCQELGCAGVVLGALDRAGRPDLTCCRTLMAAAQGMQVTFHRAFDACADQFRALEDVIGLGCQRVLTSGGQRTALAGQKQLAALVQQAGGRICVMPGAGITPAIIPEVAQQTGAHEFHASAKRLVVPGPSAQASVFDTPVWETDAAIVAELTKQLGSCQPGRVLNRGPG
>NZ_CP010056.1|WP_044518074.1|107043_108072_-|N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase
MPTRRKFLTSSSAALALLSAGRSLGAPNPSPLAATGKPLVISTWDAGLAANLGAWHILSKSGRALDAVEAGVMVTEASQNCCVGLGGNPDRDGIVTLDACIMDDKFNCGSVAALERIKHPISVARRVMERTPHVMLVGEGAQQFAVAQGFPLEPQKLSPDAEKAYREWLKTSQYQPVVNIENTGNQKKPTDPVGGANNHDTIAMLAQDAQGNLSGSCTTSGMGFKMRGRLGDSPLIGAGLFVDNAVGAAAATGQGEDVIRMAGAHTVVELMRQGLSPKAACKAAVERIAKIKGAQARDIQVCFIAISAGGEHGAFALQKGFSYAVCDAANQQQLIQSEYLLK
>NZ_CP010056.1|WP_044518076.1|108581_109550_+|tyrosine-type-recombinase/integrase
MATESQLPVIPSPPAAQVPDHLVKSTARYVESGLRGAANTVRAYAGDWKRFSTWCELHQLAALPAPVEALVGFLTELADAGKKFATIERHAAAIAKAHELAGVESPTADKKLKVLLRGIAREIKTRQKQAPAFSLESFKRTIKSIDVSVPAGLRNRALLLLGFTGAFRRSELEALNMEDLAFDDDGLVVTLPQSKTNQLGQAEEKAIFYSPDSALCPIRSLQAWLRVLERTEGPVFVSLRKNNQVTSRRMTTKYTNLIVQQYLGPQYTAHSLRASFVTVSKLNGADDSKVMNQTKHKTSAMIRRYTRLDNVRQHNSAKELGL
>NZ_CP010056.1|WP_044518083.1|109759_110815_-|Fic-family-protein
MPWQPTFLPPSQELETRPVLKKLAAAHRALAELKGIAAAIPNEGILLDTLPLQEAKDSSAVENIITTHDELYQAELQLGESQAAKEVQRYTAALRLGLGLVQQYGFLSVNHLALIQQELEQNNGGYRRLPGTTLKNQRGEIVYTPPQDNDTIMALMGNFEAYLNDDTLSEVDPLVKMAVLHFQFESIHPFYDGNGRTGRILNILYLVLKGLLDLPVLYLSRYLIRHKAAYYHHLQAVRDTGAWEDWILYLLDGVEQTARESIVLIGEMRQLMQHTKQQLRAYKFYSQDLLNHLFRHPYTRIEFVQQELDVSRPTAASYLNQLAADGVLVKYRRMTANYYVNEPLFALLARE
>NZ_CP010056.1|WP_044518086.1|111007_112888_-|gamma-glutamyltransferase
MKLISALLALMAFPLSGLAQQTQKPPLHGKHWMAITGKPLAATAGAMTFQKGGNAVDAACAMLAATCTMWDVLSWGGETQALIYNPKTKKVIALNAMGVAPTGATCEFFKDKGYNFPPEYGPLAATTPGTPGGLMHMLANYGTLSLAQVLAPALEMAAGYPIEAQTANSIEKNKALIKQWPYSKKVLLTHPGQEREAPEAGEIFVQRDLLQTLAKLVEAEKAALKQGKSRKEAIMAAYDRFYTGDIAQEFVRGSKEQGGLITMQDLARWKPLEEEPLTVNYKGVEVYKLQQWTQGPAMLQALNILENFDLKGMGYNSTKYIHTVYQAMSLSFADRDFYYGDSHVAPAEPIQGLLSKVYARQRARLIQYQKNDPGIGPGDPYPFEGRKNPYRKLLKARGFELDTTKRNFAPKHDVGNHSAPAEYQDRLWRGTTTVEAADEEGWVVSITPSGGWLPACIAGNTGVGMSQRMQSFVLDPALNPFNVVAPGKRPRVTLTPSLALKDGKPFLAFGVQGGDTQDQNLLQFFLNVVEFGMSVQQASEAANFNTNQLLLSLGGTKTEDRKPKPGQILLNESTPEPVREELKKLGYTMSFDDRTSGPINAIYFDWQHGSLWGGSSNHGEDYGIGW
>NZ_CP010056.1|WP_052430621.1|116891_120050_+|TonB-dependent-receptor
MKETRPIVRQALFLSLLTTAPLAFPGAVQAEAMRPRTADARRADTPISGRVTDEKGAGLPGVTVVVKGTTVGTSTGPEGEFTINAPENATLLFSFIGYKTQEVAVAGKTTFNLALAPDVTALNEVVVTGYQVQKKADLTGAVAVVKIDEVRDLPSSNPVRNLQGRVPGVNITTDGSPNGNAQVRIRGFGTLGNNDPLYVIDGIPTKEGIGQINQNDIESIQVLKDASAASIYGSRAGNGVIIITTKKAKKGVSRVDFSTFLTVQKPGQHIEMLNTQDYGRVYWQASVNDGITPNSPFYTFQSSTDANGSPVLDGVTVPEFLDAEKTMRTADTNWFDETQQNGIIQSYNLNVTNGGERGGMLLSLNYYDNKGTLKYTGLDRMTARLNSDYSFLNNKLKIGENLTLAKTRQSEVGIDYVRDRSSQLLAIVPVRTVDGVGWGGPVSGIADRDNPVRVLTDSKQNRSHSGRAFGNMFADLEVLKGLHLRSSLGIDYTLYKFRDIYKTYRAGYLSEANNRVTQNERFYGNWVLQNTLNYNLNLGDKHQLDLLAGHERISYNYESFFASRTNFASEDPDYVFLDAGAANQNNGGSGTAYRLASLFGKVNYSFLDRYLLSGTLRRDGSSRFGKDNRFGVFPAVSAGWRLSEEAFVKNTAPMVSDLKLRVGWGQTGNQDIADFASRGLYQSILGIQDPNFEYDRGTAYDIYGTNSNLPSGYRRVQQPNDQLKWETTTQTNYGIDFGLLDNKLTGSVDYFVKNSKDILVNLPYIGSVGEGGDKFVNGASIENKGWEFQLGYQNQLSSGLTYSVTGNLSSYRNKLTFLPAEVINAYGGNGQDVTRLGHSVNAVYGYVANGLFQNAEEVTSHATQTGAAPGRIRYEDLNKDGKIDNFDQTWITEATPDFIYGLNLGSSYKGFDLQLFMQGVQGLDAYNNVKFRTDFSFVSGENWGQRTLDAWSPTNTGSSIPASSLINTNNEGRASTYFIENASYLKLRNIQLGYSLPESVVSKVKLQGARVYVQGQNFFTIKSKELSTPDPEVTNYQYPIPVIFTTGLNISL
>NZ_CP010056.1|WP_044518089.1|120069_121794_+|RagB/SusD-family-nutrient-uptake-outer-membrane-protein
MKSIKTCALVVSLLAVSFSCSDKNFLDVSPKGTLADEDLNTPANIEKQVIAAYSQLGNDVYRAPYTSMWPYGNIRGGDAYKGGGGTGDVDAFHFYETFTFNRPDIGNTDEVWFLLYIGISRTNDALRRLNAVSEAEMPNKAVRQGEMRFLRGHFYFLLKELFGQVPYIDETIPTADYGKVSNVALNNAQLWDKIAEDFRFAEANVPASQPEIGRVTKSAAQAYLAKTLLYAAYTQNESHAVTGTDQAKLEEVVRLTDEVISSGKYSLHQDFATNFLSSGDNGSESVFAIQFSRNDGTPKGRTQRGNELNYPMNPDYGCCSFHQPSQNLVNAFKTDAQGLPLFSTFNTSGNLSDSADFKKNTVDPRLDHTVAINSHPYKYDPSFVFRNSWVRVPQVYGYFMSMKETVLPSDPSFQKNPPFMSTSKNWALIRYADVLLWKAEALIELNRPAEALPLINQIRQRAQNSTAMLRQKDGQATSNYKIGLYPADKWTQDYAREALRFERRLEFAMEGYRFFDLVRWGIAAEYLNNYFTVEKTRRQYLQDARFTKGRDEYLPIPINQINFSKGLYKQNPGW
>NZ_CP010056.1|WP_044518092.1|122058_123384_+|sugar-porter-family-MFS-transporter
MKNSNVFFWSLVVALGGFLFGFDTAVISGAEKAIQQLWGLSTVEHGFTIAIALIGTVFGAVFGGVPSDKLGRRKTLIIIAVLYLVSALGAALTSSWVAFLVFRFLGGLGVGASSVTAPLYISEVSPAARRGRMVAMFQFNIVFGILVAYLSNYLLTGTGENDWRWMLGVQALPALAFFLLLFRVPESPRWLLGRGMVEEARAILHRIDPATANQDVNSILTANALDEAGGGESLFARQYRAPVLLAVLFAVFNQVSGINAIIYYAPRIFEMTGLGKGSALLSSAGIGLVNFLFTLLAMNFIDRFGRRKLMLIGSVGLIATLGLVSRAFYLQQFGGMMVPVLLFVYIAFFAFSQGAVIWVFISEIFPNTVRAKGQALGSSTHWVMASIIAFTFPYFAEKLGGGHTFLFFCSMMVLQLIFVWRYMPETKGTSLEQLEKTLVIH

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity
NZ_CP010056_1	1.5|6905|25|NZ_CP010056|CRT	6905-6929	25	NZ_CP049908	Hymenobacter sp. HDW8 plasmid p_unnamed1, complete sequence	135546-135570	3	0.88
NZ_CP010056_1	1.5|6905|25|NZ_CP010056|CRT	6905-6929	25	NZ_CP037921	Hymenobacter sp. 17J36-26 plasmid unnamed1, complete sequence	46932-46956	3	0.88
NZ_CP010056_1	1.5|6905|25|NZ_CP010056|CRT	6905-6929	25	NZ_CP049908	Hymenobacter sp. HDW8 plasmid p_unnamed1, complete sequence	135418-135442	4	0.84
NZ_CP010056_1	1.5|6905|25|NZ_CP010056|CRT	6905-6929	25	NZ_CP049908	Hymenobacter sp. HDW8 plasmid p_unnamed1, complete sequence	135097-135121	4	0.84
NZ_CP010056_1	1.5|6905|25|NZ_CP010056|CRT	6905-6929	25	NZ_CP037921	Hymenobacter sp. 17J36-26 plasmid unnamed1, complete sequence	46804-46828	4	0.84
NZ_CP010056_1	1.5|6905|25|NZ_CP010056|CRT	6905-6929	25	NZ_CP037921	Hymenobacter sp. 17J36-26 plasmid unnamed1, complete sequence	46483-46507	4	0.84
NZ_CP010056_1	1.5|6905|25|NZ_CP010056|CRT	6905-6929	25	NZ_CP007144	Hymenobacter swuensis DY53 plasmid pHsw1, complete sequence	158549-158573	4	0.84
NZ_CP010056_1	1.5|6905|25|NZ_CP010056|CRT	6905-6929	25	NZ_CP007144	Hymenobacter swuensis DY53 plasmid pHsw1, complete sequence	159021-159045	4	0.84

1. spacer 1.5|6905|25|NZ_CP010056|CRT matches to NZ_CP049908 (Hymenobacter sp. HDW8 plasmid p_unnamed1, complete sequence) position: , mismatch: 3, identity: 0.88

ctgctgcgtgaaaaatggcactctc	CRISPR spacer
cttctgcgtgaaaaacggcactcgc	Protospacer
** ************.******* *

2. spacer 1.5|6905|25|NZ_CP010056|CRT matches to NZ_CP037921 (Hymenobacter sp. 17J36-26 plasmid unnamed1, complete sequence) position: , mismatch: 3, identity: 0.88

ctgctgcgtgaaaaatggcactctc	CRISPR spacer
cttctgcgtgaaaaacggcactcgc	Protospacer
** ************.******* *

3. spacer 1.5|6905|25|NZ_CP010056|CRT matches to NZ_CP049908 (Hymenobacter sp. HDW8 plasmid p_unnamed1, complete sequence) position: , mismatch: 4, identity: 0.84

ctgctgcgtgaaaaatggcactctc	CRISPR spacer
tatctgcgtgaaaaatggcactctg	Protospacer
.  ********************* 

4. spacer 1.5|6905|25|NZ_CP010056|CRT matches to NZ_CP049908 (Hymenobacter sp. HDW8 plasmid p_unnamed1, complete sequence) position: , mismatch: 4, identity: 0.84

ctgctgcgtgaaaaatggcactctc	CRISPR spacer
catctgcgtgaaaaatggcactgcc	Protospacer
*  ******************* .*

5. spacer 1.5|6905|25|NZ_CP010056|CRT matches to NZ_CP037921 (Hymenobacter sp. 17J36-26 plasmid unnamed1, complete sequence) position: , mismatch: 4, identity: 0.84

ctgctgcgtgaaaaatggcactctc	CRISPR spacer
tatctgcgtgaaaaatggcactctg	Protospacer
.  ********************* 

6. spacer 1.5|6905|25|NZ_CP010056|CRT matches to NZ_CP037921 (Hymenobacter sp. 17J36-26 plasmid unnamed1, complete sequence) position: , mismatch: 4, identity: 0.84

ctgctgcgtgaaaaatggcactctc	CRISPR spacer
catctgcgtgaaaaatggcactgcc	Protospacer
*  ******************* .*

7. spacer 1.5|6905|25|NZ_CP010056|CRT matches to NZ_CP007144 (Hymenobacter swuensis DY53 plasmid pHsw1, complete sequence) position: , mismatch: 4, identity: 0.84

ctgctgcgtgaaaaatggcactctc	CRISPR spacer
catctacgtgaaaaatggcactcta	Protospacer
*  **.****************** 

8. spacer 1.5|6905|25|NZ_CP010056|CRT matches to NZ_CP007144 (Hymenobacter swuensis DY53 plasmid pHsw1, complete sequence) position: , mismatch: 4, identity: 0.84

ctgctgcgtgaaaaatggcactctc	CRISPR spacer
caattgcgtgaaaaatggcactccc	Protospacer
* ..*******************.*

2. NZ_CP010054

CRISPR-Cas detection and classification

Crispr_ID: NZ_CP010054_1

• CRISPR_ID: NZ_CP010054_1
• CRISPR_location: 1715134-1715299
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 2 spacers

Consensus repeat of NZ_CP010054_1

Consensus_repeat	Method
GTAGCGCGAAGCTCCGGCTTCGCG	CRISPRCasFinder

spacers of NZ_CP010054_1

>1.1|1715158|40|NZ_CP010054|CRISPRCasFinder
GAGTAACGCACCGAGTGGTAGAAAATACCTGGCAGTGAGA
>1.2|1715222|54|NZ_CP010054|CRISPRCasFinder
TACGAGCGAAGCGAGTTCCTACGCGGTGAGCATACGCCTGTTACTCGCGTTGCT

CRISPR arrays and Neighbor proteins around NZ_CP010054_1

CRISPR arrays

The CRISPR arrays of NZ_CP010054_1

>merge|NZ_CP010054|1|1715134-1715299|CRISPRCasFinder
GTAGCGCGAAGCTCCGGCTTCGCGGAGTAACGCACCGAGTGGTAGAAAATACCTGGCAGTGAGAGTAGCGCGAAGCTCCGGCTTCGCGTACGAGCGAAGCGAGTTCCTACGCGGTGAGCATACGCCTGTTACTCGCGTTGCTCGTGCGCGAAGCCGGAGCTTCGCG

>NZ_CP010054|1|1|1715134-1715299|CRISPRCasFinder
GTAGCGCGAAGCTCCGGCTTCGCG	GAGTAACGCACCGAGTGGTAGAAAATACCTGGCAGTGAGA
GTAGCGCGAAGCTCCGGCTTCGCG	TACGAGCGAAGCGAGTTCCTACGCGGTGAGCATACGCCTGTTACTCGCGTTGCT
CGTGCGCGAAGCCGGAGCTTCGCG

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
NZ_CP010054.1|WP_044512953.1|1724825_1726022_+|phosphoglycerate-kinase	unknown	unknown	gnl|CDD|234613
NZ_CP010054.1|WP_044512947.1|1720189_1720975_-|PASTA-domain-containing-protein	unknown	unknown	gnl|CDD|275384
NZ_CP010054.1|WP_044512926.1|1710392_1712312_+|RecQ-family-ATP-dependent-DNA-helicase	unknown	unknown	gnl|CDD|223588
NZ_CP010054.1|WP_044512920.1|1706646_1707441_+|hypothetical-protein	unknown	unknown	gnl|CDD|237865
NZ_CP010054.1|WP_044512935.1|1715320_1716313_-|NADP-dependent-oxidoreductase	unknown	unknown	gnl|CDD|176190
NZ_CP010054.1|WP_044512949.1|1721188_1723960_-|D-alanine--D-alanine-ligase	unknown	unknown	gnl|CDD|234993
NZ_CP010054.1|WP_044512942.1|1717531_1717834_-|rhodanese-like-domain-containing-protein	unknown	unknown	gnl|CDD|238089
NZ_CP010054.1|WP_082017008.1|1717912_1719760_-|T9SS-type-A-sorting-domain-containing-protein	unknown	unknown	gnl|CDD|275036
NZ_CP010054.1|WP_082017007.1|1705706_1706360_+|ABC-transporter-ATP-binding-protein	unknown	unknown	gnl|CDD|213222
NZ_CP010054.1|WP_044512923.1|1707588_1710147_+|cation-translocating-P-type-ATPase	unknown	unknown	gnl|CDD|319839
NZ_CP010054.1|WP_044512938.1|1716408_1716843_-|organic-hydroperoxide-resistance-protein	unknown	unknown	gnl|CDD|274649
NZ_CP010054.1|WP_156126289.1|1703325_1704294_+|hypothetical-protein	unknown	unknown	unknown
NZ_CP010054.1|WP_044512930.1|1712905_1713586_-|type-1-glutamine-amidotransferase-domain-containing-protein	unknown	unknown	gnl|CDD|153235
NZ_CP010054.1|WP_044512933.1|1713956_1715114_-|iron-containing-alcohol-dehydrogenase	unknown	unknown	gnl|CDD|224890
NZ_CP010054.1|WP_156126290.1|1726185_1726821_-|hypothetical-protein	unknown	unknown	unknown
NZ_CP010054.1|WP_044512940.1|1716872_1717328_-|MarR-family-transcriptional-regulator	unknown	unknown	gnl|CDD|197670
NZ_CP010054.1|WP_044512915.1|1704304_1705432_+|MBOAT-family-protein	unknown	unknown	gnl|CDD|224610
NZ_CP010054.1|WP_044512928.1|1712308_1712707_-|hypothetical-protein	unknown	unknown	unknown
NZ_CP010054.1|WP_052430537.1|1713643_1713952_-|antibiotic-biosynthesis-monooxygenase	unknown	unknown	gnl|CDD|367760
NZ_CP010054.1|WP_044512951.1|1724020_1724623_-|PorT-family-protein	unknown	unknown	gnl|CDD|372635

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
NZ_CP010054.1|WP_044512953.1|1724825_1726022_+|phosphoglycerate-kinase	gnl|CDD|234613	PRK00073, pgk, phosphoglycerate kinase; Provisional.	0
NZ_CP010054.1|WP_044512947.1|1720189_1720975_-|PASTA-domain-containing-protein	gnl|CDD|275384	sd00037, PASTA, PASTA domain. PASTA domain is found at the C-termini of several penicillin-binding proteins (PBPs) and bacterial serine/threonine kinases. It is a small globular domain consisting of 3 beta-sheets and an alpha-helix. The name PASTA is derived from PBP and Serine/Threonine kinase Associated domain.	2.19764e-19
NZ_CP010054.1|WP_044512926.1|1710392_1712312_+|RecQ-family-ATP-dependent-DNA-helicase	gnl|CDD|223588	COG0514, RecQ, Superfamily II DNA helicase [DNA replication, recombination, and repair].	3.53748e-148
NZ_CP010054.1|WP_044512920.1|1706646_1707441_+|hypothetical-protein	gnl|CDD|237865	PRK14951, PRK14951, DNA polymerase III subunits gamma and tau; Provisional.	0.000235463
NZ_CP010054.1|WP_044512935.1|1715320_1716313_-|NADP-dependent-oxidoreductase	gnl|CDD|176190	cd05288, PGDH, Prostaglandin dehydrogenases. Prostaglandins and related eicosanoids are metabolized by the oxidation of the 15(S)-hydroxyl group of the NAD+-dependent (type I 15-PGDH) 15-prostaglandin dehydrogenase (15-PGDH) followed by reduction by NADPH/NADH-dependent (type II 15-PGDH) delta-13 15-prostaglandin reductase (13-PGR) to 15-keto-13,14,-dihydroprostaglandins. 13-PGR is a bifunctional enzyme, since it also has leukotriene B(4) 12-hydroxydehydrogenase activity. These 15-PGDH and related enzymes are members of the medium chain dehydrogenase/reductase family. The medium chain dehydrogenases/reductase (MDR)/zinc-dependent alcohol dehydrogenase-like family, which contains the zinc-dependent alcohol dehydrogenase (ADH-Zn) and related proteins, is a diverse group of proteins related to the first identified member, class I mammalian ADH. MDRs display a broad range of activities and are distinguished from the smaller short chain dehydrogenases (~ 250 amino acids vs. the ~ 350 amino acids of the MDR). The MDR proteins have 2 domains: a C-terminal NAD(P) binding-Rossmann fold domain of a beta-alpha form and an N-terminal catalytic domain with distant homology to GroES.	3.26818e-176
NZ_CP010054.1|WP_044512949.1|1721188_1723960_-|D-alanine--D-alanine-ligase	gnl|CDD|234993	PRK01966, ddl, D-alanine--D-alanine ligase.	1.49983e-45
NZ_CP010054.1|WP_044512942.1|1717531_1717834_-|rhodanese-like-domain-containing-protein	gnl|CDD|238089	cd00158, RHOD, Rhodanese Homology Domain (RHOD); an alpha beta fold domain found duplicated in the rhodanese protein. The cysteine containing enzymatically active version of the domain is also found in the Cdc25 class of protein phosphatases and a variety of proteins such as sulfide dehydrogenases and certain stress proteins such as senesence specific protein 1 in plants, PspE and GlpE in bacteria and cyanide and arsenate resistance proteins. Inactive versions (no active site cysteine) are also seen in dual specificity phosphatases, ubiquitin hydrolases from yeast and in sulfuryltransferases, where they are believed to play a regulatory role in multidomain proteins.	5.48553e-31
NZ_CP010054.1|WP_082017008.1|1717912_1719760_-|T9SS-type-A-sorting-domain-containing-protein	gnl|CDD|275036	TIGR04183, hypothetical_protein, Por secretion system C-terminal sorting domain. Species that include Porphyromonas gingivalis, Fibrobacter succinogenes, Flavobacterium johnsoniae, Cytophaga hutchinsonii, Gramella forsetii, Prevotella intermedia, and Salinibacter ruber average twenty or more copies of a C-terminal domain, represented by this model, associated with sorting to the outer membrane and covalent modification.	2.64081e-08
NZ_CP010054.1|WP_082017007.1|1705706_1706360_+|ABC-transporter-ATP-binding-protein	gnl|CDD|213222	cd03255, ABC_MJ0796_LolCDE_FtsE, ATP-binding cassette domain of the transporters involved in export of lipoprotein and macrolide, and cell division protein. This family is comprised of MJ0796 ATP-binding cassette, macrolide-specific ABC-type efflux carrier (MacAB), and proteins involved in cell division (FtsE), and release of lipoproteins from the cytoplasmic membrane (LolCDE). They are clustered together phylogenetically. MacAB is an exporter that confers resistance to macrolides, while the LolCDE system is not a transporter at all. An FtsE null mutants showed filamentous growth and appeared viable on high salt medium only, indicating a role for FtsE in cell division and/or salt transport. The LolCDE complex catalyzes the release of lipoproteins from the cytoplasmic membrane prior to their targeting to the outer membrane.	3.98465e-111
NZ_CP010054.1|WP_044512923.1|1707588_1710147_+|cation-translocating-P-type-ATPase	gnl|CDD|319839	cd07538, P-type_ATPase, uncharacterized subfamily of P-type ATPase transporters. This subfamily contains P-type ATPase transporters of unknown function. The P-type ATPases, are a large family of integral membrane transporters that are of critical importance in all kingdoms of life. They generate and maintain (electro-) chemical gradients across cellular membranes, by translocating cations, heavy metals and lipids. They are distinguished from other main classes of transport ATPases (F- , V- , and ABC- type) by the formation of a phosphorylated (P-) intermediate state in the catalytic cycle. A general characteristic of P-type ATPases is a bundle of transmembrane helices which make up the transport path, and three domains on the cytoplasmic side of the membrane. Members include pumps that transport various light metal ions, such as H(+), Na(+), K(+), Ca(2+), and Mg(2+), pumps that transport indispensable trace elements, such as Zn(2+) and Cu(2+), pumps that remove toxic heavy metal ions, such as Cd2+, and pumps such as aminophospholipid translocases which transport phosphatidylserine and phosphatidylethanolamine.	0
NZ_CP010054.1|WP_052430537.1|1713643_1713952_-|antibiotic-biosynthesis-monooxygenase	gnl|CDD|367760	pfam03992, ABM, Antibiotic biosynthesis monooxygenase. This domain is found in monooxygenases involved in the biosynthesis of several antibiotics by Streptomyces species. It's occurrence as a repeat in Streptomyces coelicolor SCO1909 is suggestive that the other proteins function as multimers. There is also a conserved histidine which is likely to be an active site residue.	7.33047e-17
NZ_CP010054.1|WP_044512930.1|1712905_1713586_-|type-1-glutamine-amidotransferase-domain-containing-protein	gnl|CDD|153235	cd03141, GATase1_Hsp31_like, Type 1 glutamine amidotransferase (GATase1)-like domain found in proteins similar to Escherichia coli Hsp31 protein. Type 1 glutamine amidotransferase (GATase1)-like domain found in proteins similar to Escherichia coli Hsp31 protein (EcHsp31). This group includes EcHsp31 and Saccharomyces cerevisiae Ydr533c protein. EcHsp31 has chaperone activity. Ydr533c is upregulated in response to various stress conditions along with the heat shock family. EcHsp31 coordinates a metal ion using a 2-His-1-carboxylate motif present in various ions that use iron as a cofactor such as Carboxypeptidase A. The catalytic triad typical of GATase1 domains is not conserved in this GATase1-like domain. However, in common with a typical GATase1 domain, a reactive Cys residue is found in the sharp turn between a beta strand and an alpha helix termed the nucleophile elbow. For EcHsp31, this Cys together with a different His and, an Asp (rather than a Glu) residue form a different catalytic triad from the typical GATase1 domain. For Ydr533c a catalytic triad forms from the conserved Cys together with a different His and Glu from that of the typical GATase1domain. Ydr533c protein and EcHsp31 are homodimers.	4.3831e-111
NZ_CP010054.1|WP_044512933.1|1713956_1715114_-|iron-containing-alcohol-dehydrogenase	gnl|CDD|224890	COG1979, COG1979, Uncharacterized oxidoreductases, Fe-dependent alcohol dehydrogenase family [Energy production and conversion].	0
NZ_CP010054.1|WP_044512940.1|1716872_1717328_-|MarR-family-transcriptional-regulator	gnl|CDD|197670	smart00347, HTH_MARR, helix_turn_helix multiple antibiotic resistance protein.	1.27707e-22
NZ_CP010054.1|WP_044512915.1|1704304_1705432_+|MBOAT-family-protein	gnl|CDD|224610	COG1696, DltB, Predicted membrane protein involved in D-alanine export [Cell envelope biogenesis, outer membrane].	1.42682e-45
NZ_CP010054.1|WP_044512938.1|1716408_1716843_-|organic-hydroperoxide-resistance-protein	gnl|CDD|274649	TIGR03561, organ_hyd_perox, peroxiredoxin, Ohr subfamily. pfam02566, OsmC-like protein, contains several deeply split clades of homologous proteins. The clade modeled here includes the protein Ohr, or organic hydroperoxide resistance protein. [Cellular processes, Detoxification].	5.01976e-67
NZ_CP010054.1|WP_044512951.1|1724020_1724623_-|PorT-family-protein	gnl|CDD|372635	pfam13568, OMP_b-brl_2, Outer membrane protein beta-barrel domain. This domain is found in a wide range of outer membrane proteins. This domain assumes a membrane bound beta-barrel fold.	4.38453e-15

Protein sequences

>NZ_CP010054.1|WP_044512933.1|1713956_1715114_-|iron-containing-alcohol-dehydrogenase
MKNFQFYNPVRILFGKGQIASVAQQVPAGAKVLITYGGGSIKQNGVYEQVTAALQGFDTVEFGGIEANPHYETLMQAVELARTEKVDFILAVGGGSVVDGTKFIAAAVPFQGEDPWDILSKRAKVTSALPFGAVLTLPATGSEMNSGSVVTRVSTKEKLAFSSPLTFPKFSILDPETCFTLPKRQIANGIADAFTHVLEQYLTYPVNTPLQDRQAEAVLLTLIEEAPKVLENPRGYDAMANFMWAATNALNGTLAAGVVTDWSTHYVAHELTALHGIDHARTLAIVLPAMLRYRRAGKLQKLVQYGQRVWNITAGTDEERAEAAVQATVRFFESLEIKTRLSDYEVGEETIRHIVQRFTERGVKNLGERADVQIADVEQILTLSL
>NZ_CP010054.1|WP_052430537.1|1713643_1713952_-|antibiotic-biosynthesis-monooxygenase
MPKPEDIYCVAAEWLVQPGQAETVRRLLKEAAAAVRQHEPGNLVYTAHQSAEEPERFFIYEQYVDQNAQLTHRAAPHFQDLVLGQIVPLLAERKTTFYRLLL
>NZ_CP010054.1|WP_044512930.1|1712905_1713586_-|type-1-glutamine-amidotransferase-domain-containing-protein
MNILMVLTSHDQLGNTGHKTGFWLEEFAAPYYVFKDAGATLTLASPAGGQPPLDPKSDDPSAQTEATERFKKDSEAQKALASTVKLDSVSAADFDAVFYPGGHGPLWDLAEDKKSIELIETMYAAGKPVAAVCHAPGVLRHVKAPDGSSIVKGKSVAGFTNTEEEAVQLTNVVPFLVEDMLKQNGGNYSKGADWQPYIVTAGNLITGQNPASSEPAAKELLKQLAK
>NZ_CP010054.1|WP_044512928.1|1712308_1712707_-|hypothetical-protein
MADLLYHTARKHNISEITLNVLTGQIEPVALQTAPFESNLIRIKTLLDTRLQDLGFNSGFIKEVKFRIEVPANKLLLFCWPSMQDLIGRSYETKRMAINVYATHMNEVYPKPELPHFSLWKAIKYLFQQNYS
>NZ_CP010054.1|WP_044512926.1|1710392_1712312_+|RecQ-family-ATP-dependent-DNA-helicase
MPHPTDDILHVLRQTWGHTQFRPLQEDIIRSVLAGQDTLALLPTGGGKSICFQVPALARPGLCLVVSPLIALMKDQVENLRKRGIKAEAVFSGMTHTEIDQTLDNCVYGPVKFLYVSPERLLTDMFRARVGKMKVSLLAVDEAHCLSQWGYDFRPPYLKIAELRELLPGVPCIALTATATEQVRQDIVEKLHFGATHRLFQQSFARPNLSYSVLSTEDKLKRLLEVVRGVGAEKTSIVYARTRRQTEDTAAFLQQHNVVAAAYHAGLPAEQRTRTQQDWMGNKTRCIVATNAFGMGIDKPDVRLVVHLDAPDNLEAYYQEAGRAGRDEKYAFAVLLSGPNDADELRRRTRQAYPPLDVVRRVYQALANFSRTAVGGGELVAFDFDLQQFAETYRLKALDAHNSLRTLAREGFVQLNEAVNNPARVHIPINHTDLYRFQVANQQHDQLIKSLLRFNGGELFAGFQRISENSLAQHLKLSVVDVRKMLLFLHRSGIIQYQPKHESPQALFTTPRFDADKLPLDQKRLLQAQELAVHKTESVIRYTAGGRCRQQLLLEYFGELDAPACGVCDFCLAKKKARQDAAPTPALREQLLKLLRTTPQTPREVLAHFVPGQAPAVTELLRELVERGELKYAPDGRLG
>NZ_CP010054.1|WP_044512923.1|1707588_1710147_+|cation-translocating-P-type-ATPase
MATASETAILDATPATLPPGLTPAEVEQQRQRFGPNKLTDNAGNRTWQILRGVVTEPMFVLLVVTAGVYMVIGSYQEGFTMLGAIVLVAGISLYQEARSDKALRALQQLSQPLAHVRRAGEQLDVPAEDVVVGDVLLVSEGEQVVADGLLLEAHDAQADESFLTGESLPVDKVADGAQAVYLGSTLTSGAVWARVTAVGSQTRLGKIGGLMQAVKPEQTPLQRQIAVFVRRMALVGIVAFVLVFGYNWWESGSLVHGLLHGLALAMAVLPEEIPVALSTFMALGAWRLMKQGILTKQPQTVEALGSATVIGVDKTGTLTQNRMTLTRAYCPDDTVPWEIRPELVLAPAQARLLATAMWASEPQPFDPMERALHEAYAQFSSVDERPRFQLVHEYPLGGHPPMMTHVFAAADGRRIIACKGAPEGIMALSHLSAAEQQAAQQALESLTSVGLRVLGVAWSEWTSTEYPERQQDFNWQFAGLVGFSDPPKANIPQVLEQFYQAGIEVKIITGDNAVTAAAIARQIGFRHPAPVLTGAEIMALDEAALNQRVQEVGLYARMFPEAKLRVINALKANGEVTAMTGDGVNDGPALKAAHIGIAMGHRGTELARQAASLVLLDDDLSRMVTAIGFGRRIYDNLKKAVQYIISIHIPLILTVLVPSLLAWKYPALFGPVHVIFLELLMGPTCSIVFENEPMEAGTMHEKPRPASDTFLSFRELGLSLVQGSVAAAGVLGVYFYSLQVAHSEILTRTLVFTTLVLANVVLTLVSRSRHLTVLTTLRYPNPLVPLALGITLALLALCLTFAPMQHLFQLQPLSLIQLAICAAVALVSVGWFEVYKAVRYRRAAAIERTASK
>NZ_CP010054.1|WP_044512920.1|1706646_1707441_+|hypothetical-protein
MNEVTKNTKEPARKMKVESYDIARSDETLHLATDLAKFIKDNKLTTSVQGKEFVNVEGWQYAGSRLGIVPIVDHVINVSTESEIKYQAKVTLFDLRSGHTVGAGFAICSNKEQGKKFYQEFAIMSMAQTRAIGKAYRNILAWIIRAAGYEPTPAEEMEYHGNTPEVKAAPAPSAAPAPAAPTAMKAVPAEPVAPVAEPAPAVAYATAAQKEEIIRLLNHPVITRQEKTKMLLNINRLDEERAVQAIAKLKKAIEDRENGETAAA
>NZ_CP010054.1|WP_082017007.1|1705706_1706360_+|ABC-transporter-ATP-binding-protein
MLQAINIRKSYNTLEVLKGIDLTIKKSEIVSIVGSSGAGKSTLLHILGTLDTPDSGEVLFDGENVSALGRSSLARFRNRHIGFIFQFHNLLPEFTALENVCLPAYLAGRSEKETRVRARELLGMLNLERRADHKPSEMSGGEQQRTAVARALINSPEIIFADEPSGNLDSQNAQELHQIFFLLRKELGQTFVIVTHNDQLAAMADRTITMKDGYISE
>NZ_CP010054.1|WP_044512915.1|1704304_1705432_+|MBOAT-family-protein
MLVLHTIVFFSLLLLVPAAWLLPERWQISAVSLLTAAFLLYVAPISALMLLLTAAASYWVFKSSWLRPTGAVLLAIGQAVAVWMVFKSEWPTQAGWWTQVGVPLGLSYYTFRQIHYAIERYKQTLPRHTWADYLRYLFFLPTMLVGPINRFPDFLRDVRRRRWDAALASVGLERVLYGYVKVVVLGNALLTNKLGHLLTALEPQHPALATYLGILRFAGNAYVQFAGYSDIAIGLSLLLGFRTGENFAAPFLAPNLNDFWKRYHISLSQWCRDYVFSPLLSLTRRPMLAIAASMLVLGLWHEWSGRYLVWGALQGLGILFWHQYQKFAEKRPNVVPVLPVWLGVFLTLHFYCFSCVIIREPSWQAVAHTFYRLLS
>NZ_CP010054.1|WP_156126289.1|1703325_1704294_+|hypothetical-protein
MRARNSPPAEPAPIWVWSVALGMLLSFGTVYGWGFVHSVSTYHQEVVAGARQLLQREWQDRTDSVTRVVILGSSLTGNGVMPSAYFTRKTNGRCRVVRLFRISANLESFTDRAPILKYLQEYPPDILCIEENLLLYDLKDWSELSPDSFLLKNVARYSYQLVNRSKQRLGLTHEVIDVNRPGLAMQDVDMPAPTTPWDTTHLTEFVAEIRQRKVRPFAPRHPVHQALRILRGQGVRIVLLHFPRPAPLEKAIYSGRNGDSLRYRIAQYQRAYQASYWHEPGPYHFRYFYDKAHMNFQGRALYSTWLAKKLRQETNPTLLTAR
>NZ_CP010054.1|WP_044512935.1|1715320_1716313_-|NADP-dependent-oxidoreductase
MKTILLASRPQGAPTLANFRFEEQEIPQPQAGQVLLKMRYASVDPYMRGRMSDAKSYVAPFEVGQPINGGVVAEVVESHNEQLPVGSIVVGNLPWQEYCVSGGQGLTRVPANQAPLSYYLGLLGMTGLTAYFGLLDICQPKPGETVVVSGAAGAVGMVVGQLAKIKGARVIGTAGSDEKVAELKKLGFDEAINYKTTPDIAQALAAAAPRGVDCYFDNVGGAITDAVYDLLNKHARIALCGQISMYNATSVPMGPRPEPKLLKTSALLKGFIVSDYLERWPEGIQHLAEWYQQGKLQAEETITEGFDQLPAAFLGLFQGENTGKAIVKVA
>NZ_CP010054.1|WP_044512938.1|1716408_1716843_-|organic-hydroperoxide-resistance-protein
MKLEKKLFTAQAKAKGGRDGHVSSNDEVLNIALSTPKAMGGPGKTGATNPEQLFAAGYAACFEGALGVAARQAGVRLEGVTVEGLIGFGQAEDGGYGISADLHVNIPGLAQSQAEELVEAAHQICPYSRATRGNIEVNLHTTTN
>NZ_CP010054.1|WP_044512940.1|1716872_1717328_-|MarR-family-transcriptional-regulator
MSNSDPTPTPDPSWLKLENQLCFPLYAVSRMLTKAYQPLLQELDLTYPQYLVLLLLWEHEQLTVKALGDNLLLDSGTLTPLLKRMEQKQWISRHRDPQDERSVIISLLPAGRTLQNSACRIPEKMLEKLHLSPAEVDNLRQQLNTLLARLT
>NZ_CP010054.1|WP_044512942.1|1717531_1717834_-|rhodanese-like-domain-containing-protein
MPDITPAELKQRQAEGTAPIIIDVRETWENEEGHIEGSRNIPMGELPGKLDELEGLKNQEVVVHCKGGGRSASAKAYLTQQGFTNVRNLLGGFQAYQQAQ
>NZ_CP010054.1|WP_082017008.1|1717912_1719760_-|T9SS-type-A-sorting-domain-containing-protein
MMRSSFLLCLLLTWATQLRAQNVQPLSADPGRASPVGNASHSAARGTSIALPFFDDFAQQPEGTPNAEHWEPRGGTLVNNRFPVAPPSRNVVTFDGLKANGQPYGSSSFYSDTDTLTSLPIDLSGLTAGSNVYLSFFWQAGSIVGSPSAASSTRQVAFTLEFLNDAGQWQSIWSQRSTGRRTDFAQEIVAVTEPQYLHANFRFRFHSIGNQANTRDAWSLDYLKLDRNRSAADTTYRDIATSAPLTSLLKRYASMPAVQLAAAPTPADELNDQTGTTINNFDIGPAPTPITWRGTAQNLPRGPELQFLTGNRSIDASARQVPIMGDVRTVAASLLTGGPQRIRHRILLGTNETDSRIQPNDSISRVTELSDYYAYDDGTAEAAVSLPALTGGASTFFAYRIDLNQPDQVRSVRLYPVLPNAAGRAITLSVWADENGKPAEKPLASVPYTVPATAPAGGFLDVPFSQPVGVSGTFYVGYGQASLGQFVQFGLDLNSSPPANYFFYQVRNEWSAVTDFSGALMLRPVMTGTVTAATPSRAAALLTLYPNPSTGIVQVKGRYTHATVLDALGRTVWQQTGLAQGQPALDLSRLPAGVYFMRLALPDGSLATKRLVLTR
>NZ_CP010054.1|WP_044512947.1|1720189_1720975_-|PASTA-domain-containing-protein
MSFFKSDTPLDVLKHLLAMAAVVAIMVLGFFYVYLPMTTNHGETIVVPKITGMQQTDLEDYLDERNLLFFVDDSSYNPGTRPGTVLTQDPAPGEKVKEDRKIYISVSMKNPPVIKMPRLTDGSVKNAQMILKSYDLVVGQIQLVPDLAQNAVLKQLVNGKEIAPGAPIAKGTKVDLVVGDGQGNQEFPVPNVVNMPADEAATLLAGQGLQVGEIFYQAAEEGQEDGNVVKQRPVSSPGATIRTGQLVDLWVAGQAPLKPVE
>NZ_CP010054.1|WP_044512949.1|1721188_1723960_-|D-alanine--D-alanine-ligase
MKIGIFFGGPSREREISFAGGRTVYDNLDKSLFQAVPVFVDSRGNFILLDWHYIYKGTIRDFYPPVSALPASEHKLQVYLESLGELSLAQQDEIINQVGRRIQPQELSSLMDFAFLALHGPGGEDGAIQGLLEWYGIPYSGSGVLPSAFGIDKIAQKKLLHALNRPTPEFRVVSAEEWDAADPAATLAYLERELGLPLVFKAPRQGSSIGISILRTANVEAFHRAMERSLFRKTVTRDDWQRLSERDKIMWVQQLTDIREGIGLPVMLDADDEHIIYHPEALLNTLAERLQTAEQVRLTNVDGETQVLVESFVQGREFSCIVVEDPNGQPLALPPTEIVKGEEMFDYRSKYLPGLARKITPIDLPEDEIQRIREACEEMFRTFGFQVYARLDGFISSQLPVAGSQISGEETGNSQPATENSIFLNDPNTTSGMLPASFFFHQAAEIGLNPSQFLTYLIRTSLAARRRAGMRPVQLQRLLRGLDAAVAARGHEERQRIKVAVIMGGYSSERHISVESGRNIYEKLSSSIKYEPVPVFLTGNNQDFRLYVLPINVMLKDNADDIKEKVEHMEAGHSLHPILERIRREAEAITSTYAGQPTAQPRRVSFEELAKMVDEVFIALHGRPGEDGALQQQLEKFGLPYNGSGVGSSSITINKFETNRRLREAGMRVAEHRMANRLEWQADAEGFYRSLETQFPYPFIAKPADDGCSSAVKKVKNRPELEAFTRLIFREQEDLMIPDAETLHLGFKEEFPRKDAFLVETLIERDGAAHFLEITGGLLTHWRSDGQLDIEVFEASEALATGEVLSLEEKFLAGEGQNITPARYAADAIERQRISDEVKEELRRVAEILNIQGYARIDAFVRVRDNGAVEVIIIEVNSLPGMTPATCIFHQTALAGYTPYDFIDQILEFGKERTRKEKLAVNS
>NZ_CP010054.1|WP_044512951.1|1724020_1724623_-|PorT-family-protein
MYIRFLLAVPALLLSQLAVGQHLRFGVKAGANYSRDKVKHTVGPDRLLGINGGLVARYSLTEDGFWSIQSELLYSAKGAKDKYGSTTTGARTTNYYHRNYLDLPILTKINAKGLTFEAGPQLSYLLSFKNDVNNDLYEQPQRRDFNSLQLGYVAGIGYELPSGPNITLRYCGDITHPLQYSPTRNSVFQAQLGYMFGAKQ
>NZ_CP010054.1|WP_044512953.1|1724825_1726022_+|phosphoglycerate-kinase
MKTLDQYNFAGKKAVVRVDFNVPLDSDLRITDDTRIRAATPTVKKILADGGSVILLSHMGRPKGGPDKKNSLRNLVLRLQQEYGQEVKFADDVLGQEAAQMAASLQPGEILLLENLRFYAEEEKGDEAFAEKLARLGDVYVNDAFGAAHRRHASTAVMAHHFTPENRVAGYVMQGELENAKRVLDHAERPFTAIMGGAKISDKIQIIEQLLDKVDNLLIGGGMSYTFAKAEGGQIGNSLLEGDKMDMALDLMRKAKEKGVNLVLPVDSIIANQFANDADIDVAGSHSIPATWMGLDIGPETRELFAEIIRNSKTILWNGPMGVFEMSNFSVGTEFVARAIAEATENGAFSLIGGGDSAAAVNQLGFADKVSYISTGGGALLEYMEGKELPGVAALEGR
>NZ_CP010054.1|WP_156126290.1|1726185_1726821_-|hypothetical-protein
MLLEQNRILLGTTQLIMREHGLYACQRSTQNIPLLEVEVPYEKILPVQLERTRQVPTRWLLYMLALIVWVAIEPVQESWATGTVTPGLVGVLVFLAVLGAFIALEWDRRWFLLHFRTAQLNLTFADRRRDRAQFYTFAHTLEQRVKEYLRTHYARINPLGPIEQQVRRLQWLQEMQVLSEPEARALMVRLTGRQQETLQGMGHELEGLYEN

Prophage detection

Region	Region Position	Protein_number	Hit_taxonomy	Key_proteins	Att_site
DBSCAN-SWA_1	3172257 : 3179100	8	Microbacterium_phage(16.67%)	tRNA	NA

Sequences of DBSCAN-SWA_1

Nucleotide sequences of DBSCAN-SWA_1

>NZ_CP010054|3172257:3179100|DBSCAN-SWA
CTTACCGGCGTCCCAACTGCCGCAAGTGCGCTACGTGTGAAGCCACGGCATACCGCATGTTCGGATACTCGTTGTACTCAATCCCGAACTCCAGGCACATCTGGCGCAGAATCTTGTTCAGGGCCGGGTAGTGCACGTGGGAGATGGTGGGGAAGAGGTGGTGCTCTACCTGGAAGTTGAGGCCGCCTACCAGCCAGCTGATGATTTTGCTGTCGGTAGCGAAGTTGGCGGTGGTTTTAATCTGGTGAATGGCCCACTCGTCCTCAATCTTGTTAGTGGTGGCGTGGGGCACTACAAAGCTGGTGTGCTCTACGGTGTGCGCCAGCTGGAATACAATGCTGAGCGAGAAGCCGGCCACGCAGGCAAACACCACAAAGCCAATCAGCCAGGGCAGGAAGCCCACCATATAAATAGGCAAGGCCACAAACAGGCCCAGGTGCAACACCTTAAAGCCCCAGAACACACCCTCATCGGTGGCGGTCATTTTCTTGATGGGCATCTCCCCTATTTTCCGCTGGAAATACTTCTGGTAATCCATGTAGAAAATCCAGGCAATGAACAGCAGGGCGTAGAAAAACCAGAAGTACAGGTGCTGGAAGCGGTGCAGCATGCGGCGGGGCTGGTCGGGGCTGAGGCGCAGCCAGGGCTGGGCGTTCAGGTCATCGTCTACGCCTTCCACGTTGGTGTACATGTGGTGAATGAGGTTGTGCTTGGCATTCCACATGAAGGAGCTGCCGCCCATTACGTTCAGGGTGAAGGCGGCAAACTGGTTCAGCCACTTGGATTTGCTGAAGGAGCCGTGGGCGCCGTCGTGCATTACGTTAAAGCCAATAGCGGCGCCAATACCACCCAACAAGGCGCATTCCAGCACGGCAACCCAGGCAGGCGGCGTGAGGAACACAATGTGCAGATACACGGCTACGAAGGAAGCCGTGAGGAGAATGGCTTTGAAGTACAGGCTTTTATTGCCGGTGGGGGCAGTGCCCGCCTCGGCGAAATAGGCATTGGTGCGGCGCTTAAGCTCCTGGTGGAAAGAGCGTGACGCGGCAAATTTGGGTGCTTGCATTGAAATGGGAAAAAAGAATGGCGACAAAGGTACCGCCAACTGCTGCGTTAACGCCGCGTAAGCCGTAAAAAAGCCGGATTTTATAAGTCAGGGGTGTCTCCCAGTCTGATGTGGGGCACGGCCGAAGGGCTTTATACTCTTTCAGGACAGGCTCAGCAGTAGAATGGTTCAGAAGTGGGGCGCCGCCACGGGCAGCCTGCGGGCTTCCGGTGGCGGTAAAAGGCTGGTAAGCGGAAGCGTTGTGCTTAGCTCGACAGGTAGGTAACAGCTATACGAGGAAAATGGCTGAGCGGCTTCATGTACAACATATTGGCACGCACGGTCCGGCACCCGGAGTGCCGCGTATGTAGGCACACTGTTCTTTCATTTTTACTGCATTTGTACCTGATATGAGCTACATCAAAGCCGGCCCCGACGCCAATGGCCACCCCGTAAAGCTGCACTATACCGACCTGGGCCAGGGCGCCCCCATCGTCCTGATTCATGGCTGGCCCCTGAACCACGAATCCTGGGAGTATCAGCTGAAAGAGCTGCCCAAGCACGGCGTGCGGGTTATTGCCTACACGCGCCGGGGCTTCGGCAACTCCGATAAGCCCTGGGACGGCTACGACTACGACACGCTGGCCGACGACCTGAAAGCCGTACTGGACGAGCTGAACCTGCAGAACGTAACGCTGGTGGGCTTTTCCATGGCCGGCGGCGAAGTGGCCCGCTACATGAGCCGGCACGGCGGCGCCCGCGTGAGTCGGGTGGCCTTTGTTTCGGCCGTAACACCTTTCCTGCTGAAAACCGCCGACAACCCGGATGGCGCCCCGGAATCAGCCTTTACCGGCATATTTGAAGGCCTGGAAAAAGACCGGCCCGATTTCATGCGGTCCTTCGCCAAGAAATTCTACGGTGTGGGCCTCATCAGTCACCCCGTGAGCGACGCTACCCTGGACTGGAACCAGTCTCTGGTGATGCTGGGCTCACCGCGCGCTACCGAAGCCTGCGCCCACTCCTTTGCCGAAACCGACTTCCGTCAGGACCTAAAGACTATTAAAGTACCAACCCTGGTTATCCACGGCGACCAGGATGAGACCGTACCGATTGAAGTAAGCGGCGCCCGCATGCAGGAGTTTGTGCCGCACGCTACCTATAAAGTGTACAAAGGAGCACCCCACGGCCTTTTCATCACGGAAAAGGACAAGCTGAATGAGGATATCATGGCTTTTGCGGCCGGCAAAGCGTAAGTGTCATTCCGAGCGAAAGCGAGGAATCTGAGTTTGATGACCAGCTTGCGGAAACAGCGTCTGTGGGCAGTCCGGCAACCTCTTCCTTCAGATTCCTCGCTGGCGCTCGGAATGACAATTTTCTACCAACGAAAAACGGCAGCGAGCTTTAGAGCTTGCTGCCGTTAGTGTAGATAGGCGTGCCAGTTTTCATCAATGGTACCGGCACTGAGCTTGAGGAAACCCGAAAGTGTGGGTTTTTTGGGCTGTTGCCTGATGGTGAGCCCGGCTTCATGGGGCGTTCGGTGGCCTTTGGCGTGGTTGCAGCGGGAGCAGGCCGTGAGCAGGTTGCCCCAGCTGGAGTCGCCGCCGCGGCTGCGGGGCAGCACGTGGTCCAGCGTCAGGTTTTTGGTGGAGCCGCAGTACTGGCACTCGAAATGGTCGCGCTTCATGATGTTGTGCCGGCTCAGGGCAATGCCTTTGTAGGGCACGCGCACATAGCGCTGCAGCCGGATGATGCTGGGCTTGGGGTAAGCCTCTGAAATAGTGCGCAGAAAACCACCCTCCGACTTGGCAATCATTTCGGCTTTGTCGAGGAAGAGCAGTACAAACGCTTTCTGCACGCTGCAGAGCGTTATGGCGGTGTAGTCGCCGTTTAGCACAAGCACTTTCTGGTCCATATACACGGTGGCAAGAATCATCTGAGGAGTAACGAAAGCTAGATGATTCTAAGCGCATTAACAATAGCTACCACGTTTAGTTCGAGGGAAAAACCGGCCTTGTAAGGGCAGTAGTATTCATTTCGTTGAGCGCGGAAAAGGCCCCGGGGTTGGCTCCGCCGCGTGCTGCCGCTTCCGCTGCGGGCAGGGCGGTTTCCGGGCGGCGGGTCCACGTCACAGTTTCTTGATGGAAGGGTAATATTAAGCTGCTCTTTCCTACGGCCGCGGCCTTCACCTTTGTGTCGGATATCCAAGTGAAAAAGAATCAGCCACCCTTACCGAAGTCTATGAAACCCTTCCTGTCAACCTCCCGCAAGCTGCTGCCGCTGCTGTCCCTGGCAGCCCTATGTCTGGCCGAACCTGTTTTTGCTCAACGCGCAGAAGAAACCAGCCCCCAGCCCGTGGGCATTGAGCGGGTAGATACCTTCCGCTACCTGGTGCGCATCAGCAACCCGGCCCTGCAGAAAGCCCGTCTGCAGCTGGTGCGGCTCTCCGACAGTGTGGTGCTGTATGAGGAAAACAGCGCCAAAGCTACGTTTGGCCAAAAGCTGAACGTACAAAGCCTGCCCGATGGACAATATGCCATTGTAGTGCGCGTGGGCCCGCAGCAGTACCGGTATACCCTGCAGCTGCAAACCGAAACCCACCGCACCACCGACCTAAGTACTGCCAGCGTAGTACTGCGCCCCACCAACAAACTTCGCTAACCGCCGCCCCGATATCCGCTACCTGTTTATCGTATTCGCTATAGCCATCCATCATTCGAATTGCTATCATAAAAAAACGCCCCTGCAGCAAGCGGGGGCGTTTTTTTGGTGGATTACGGAAGAGACTTAGTCGGCCAGCAGGCGCTTAATCAGGCGCAGCTTGTGGGAGTACTTCTGCCGGTCGCGGTTGAAGATGCCGTTATGGTCGAGCGGGTCGATGCGGACTTCGCCGTGGGCGTGGAGGATGGTCTGGTCGTCGAGGATCAGGCCTACGTGCACAATGTTGCCGTCGGCGTTATCGAAGAAGGCCAGGTCGCCGGGGCGGGACTGGGCCACGAAGTGCACCGTCTGGCCGTGGTCTATCTGCTGGCGGGCATCGCGCGGGAGCGGCACCCCAATCAGGCCGTAGAGCTGCTGCACCAGCCCGGAGCAGTCGAGCCCGAAGATGCTTTTGCCCCCCCACACGTAGGGCGCTTTCAGGTAGAGCTGGGCAATTTTGATGAGCAGGCGCAAGCGCTGGTCCTGAGGGCCTTGGGGGCCGTGGCCGTTCTGGGGGTTGGTAGCCGCGCCGTTGTAGAAGTACTGGTGCTGGCCCAGGCGCAGCGTCATGCCATCGAAAAACGGCAGTCGGCAGCCCAGCGTCACCGGAATGCGGCTTTTGCTCTCGCTTACTATCTGCACCACATCCAGGCTGCGGGGGTGGTCCTGCTGCTGCCAGGCAGTGAAATACTCGCTGGTGACGGGCGTGTGCTGTTTAAAATCAAGCCAGCCCACGTACTGGTCGGCGGCAATACGGATCTGGTGCCACTGGCCCTGCGTCTGCAGAATGGTATAGCATTCGCCAAACATCAACTCCGTTACCAGCTCGGCTTTATCGGTAGGCTCGGCGCGTACCGGCACGGCGCTCAACGCGCAGATTCCGTATTCCAAAAGGTGAAATTGTGAGGTGTGAAGTTGTGAATGGCTGGGTAGCAACGCGCATTCCCGTTAAGCAACCTCAGCAGTAAAGTAATACCTGAGCTTGTGCCTGTAAGCAATGCGGCAAATATCCGCTCTAAAACTCACAATTTCACAAACTCACTATTTCACTTAATAGTCCCGCAGGCGGTCCATTTCGCGCTTCTGGTCCTTGGCTTTCAGGTCTTCGCGCTTGTCGTAGAGCTTTTTGCCTTTGGCCAAGGCTATTTCCAGCTTGGCAAAGCCCCGATCGGTAACGAACAGGCGGATGGGAATAATGGTGAGGCCCTGCTCCTGACTTTTATCGGCCAGTTGGCGCAGCTCCCGCTTATTAAGCAGCAGCTTCCGCTCGCGCTTGGGCTCGTGGTTATTGTAAGTGCCCAGGGCGTACTGCGCAATGCTCAGATTGTGCACCCACAGGCTGCCATCGGAGTGAAAGGTGCAGAAACCGTCCTGCAGGTTCACGCTGCCGTCGCGGATGCTTTTAATTTCGGTGCCCTGCAGCATCATGCCCGCATCATACTTCACCAGAAACGCATATTCGTGGCTGGCACGGCGGTTCAGAATGCTGATGCGCTTGGGAGTATCGTCTTTTTGTTTTGCCATGAGGGGTTTGCCGCCGGTGGGCGGGTAATGCAGACTTCGCAGATTGGCCCGGTAAGGTTGCGCGGCCCGCAGAAAGAACGGGAGAAACCGCTAATCCGCCGACTCGCGCAACAACGCAAAGCGGGCGGCCAGCCGGGCACCGTCTACAATGCGCTGGCCGGTGCGGCCGGTAGCCACCAGCCGCTCGCCTACGCGGGCTTCTACGTGGCAGGTCAGCTCCTGGCCTTCCAGATGCTCAAAAGTAGCCTGAAATTCCACGGTTTCGCCTTCAAAAGCCGGCGCGTGGTGCTTTATGTGGAGCATGGTGCCAATTCCTTCCTCGCCTTCTTCCAGCATTTCTTCCACAAACTGCCGGCTGCTCCACTCCAGGGCCTGCCCCAGCGCAAACGTGCTGAGCACGGCATGAATCAGCTTGCCATCCAGCATGGCAAAATCCGAAGGCTTTACCTGGATGGAATACGTGCGGACGTCGCCGGGGAGAAAGGGGTTTTTCATTTCAGTTGCCAGTTGCGAGTTGTCAGTTTTATAAGTGTCTGTTGAACGAACTGTCAACCGACAACTGGCAACTCAGGAGAGTTTCAACCGTGGGTCGGGGCTGGTGAGTTGGGTGGTGAAGTCGCCGGCCAGATACTGGAAATGGGCCGTCATGGCCACCATGCCGGCATTATCGGTGCAGTACTGAAATTCCGGGATAAACACTTCCCAGCCCTGCGCCAGGGCTTCCTGCTGCAGGGCTTCGCGCAGGCCGGAGTTGGCCGCCACGCCCCCGGCCAGGGCAATGTGTGTCAGGCCCTGGTCGTGGGCGGCGCGGCGCAGCTGGCGCAGCAGGGTTTGGATAATGGTGTGCTGAATGCTGGCACAGAGGTCAGCCTGGTTTTCCTGGATGAAGGCAGGATTTTGGGCAGTTTCCTTTTTCAGGAAGTACATCACCGCCGTTTTCAAGCCGCTGAAGCTGAAATCGTAGCCAGGCATGGCACCTACCGGGAAGGGGAAGCGCGTGGGGTTACCCTGCCGCGCCAGCTTATCCAGGTGGGGGCCGCCGGGATAAGGCAACCCCAACAGCTTGGCCGTTTTATCGAAGGCCTCGCCGGCGGCATCATCAATCGTCTGGCCGATGACCTGCATTTCCTGGGGGCTGCGCACCACTACTAACTGGGTGTGGCCGCCGCTTACCGTGAGGCACAGAAAGGGAAACGCAGGCCGGGGCTCCTGAATAAAGTGCGCCAGAATGTGAGCCCGCATGTGGTTCACGGCAATCAGCGGCTTATCAAGCCCCAGTGCCAGGCTTTTGGCAAACATGCTGCCGACCAGCAGGGAGCCCAGCAGGCCAGGCCCTTGAGTAAAGGCCACGGCATCCAGCTGGGTTTTGTGGATGCCGGCGCGCTGCAGTGCGGCTTCCACTACCGGAATAATATGCTGCTGGTGGGCGCGGGAAGCCAGCTCGGGCACTACGCCGCCATATTGCTCGTGCACTTGCTGGGTGGCTACCACGTTGGAGCGGATTTCGCCGCCCACCATCACGGCAGCCGAGGTATCGTCGCAGGACGATTCAATAGCCAGGATTACGGGTGCATTCAT

Protein sequences of DBSCAN-SWA_1

>NZ_CP010054|3172257:3179100|3175539_3175959_+|WP_044514729.1|DBSCAN-SWA
MKPFLSTSRKLLPLLSLAALCLAEPVFAQRAEETSPQPVGIERVDTFRYLVRISNPALQKARLQLVRLSDSVVLYEENSAKATFGQKLNVQSLPDGQYAIVVRVGPQQYRYTLQLQTETHRTTDLSTASVVLRPTNKLR
>NZ_CP010054|3172257:3179100|3178089_3179100_-|WP_044514741.1|tRNA|DBSCAN-SWA
MNAPVILAIESSCDDTSAAVMVGGEIRSNVVATQQVHEQYGGVVPELASRAHQQHIIPVVEAALQRAGIHKTQLDAVAFTQGPGLLGSLLVGSMFAKSLALGLDKPLIAVNHMRAHILAHFIQEPRPAFPFLCLTVSGGHTQLVVVRSPQEMQVIGQTIDDAAGEAFDKTAKLLGLPYPGGPHLDKLARQGNPTRFPFPVGAMPGYDFSFSGLKTAVMYFLKKETAQNPAFIQENQADLCASIQHTIIQTLLRQLRRAAHDQGLTHIALAGGVAANSGLREALQQEALAQGWEVFIPEFQYCTDNAGMVAMTAHFQYLAGDFTTQLTSPDPRLKLS
>NZ_CP010054|3172257:3179100|3177612_3178017_-|WP_044514738.1|DBSCAN-SWA
MKNPFLPGDVRTYSIQVKPSDFAMLDGKLIHAVLSTFALGQALEWSSRQFVEEMLEEGEEGIGTMLHIKHHAPAFEGETVEFQATFEHLEGQELTCHVEARVGERLVATGRTGQRIVDGARLAARFALLRESAD
>NZ_CP010054|3172257:3179100|3177048_3177522_-|WP_044514736.1|DBSCAN-SWA
MAKQKDDTPKRISILNRRASHEYAFLVKYDAGMMLQGTEIKSIRDGSVNLQDGFCTFHSDGSLWVHNLSIAQYALGTYNNHEPKRERKLLLNKRELRQLADKSQEQGLTIIPIRLFVTDRGFAKLEIALAKGKKLYDKREDLKAKDQKREMDRLRDY
>NZ_CP010054|3172257:3179100|3174718_3175213_-|WP_044517148.1|DBSCAN-SWA
MDQKVLVLNGDYTAITLCSVQKAFVLLFLDKAEMIAKSEGGFLRTISEAYPKPSIIRLQRYVRVPYKGIALSRHNIMKRDHFECQYCGSTKNLTLDHVLPRSRGGDSSWGNLLTACSRCNHAKGHRTPHEAGLTIRQQPKKPTLSGFLKLSAGTIDENWHAYLH
>NZ_CP010054|3172257:3179100|3176085_3176859_-|WP_048826433.1|DBSCAN-SWA
MPVRAEPTDKAELVTELMFGECYTILQTQGQWHQIRIAADQYVGWLDFKQHTPVTSEYFTAWQQQDHPRSLDVVQIVSESKSRIPVTLGCRLPFFDGMTLRLGQHQYFYNGAATNPQNGHGPQGPQDQRLRLLIKIAQLYLKAPYVWGGKSIFGLDCSGLVQQLYGLIGVPLPRDARQQIDHGQTVHFVAQSRPGDLAFFDNADGNIVHVGLILDDQTILHAHGEVRIDPLDHNGIFNRDRQKYSHKLRLIKRLLAD
>NZ_CP010054|3172257:3179100|3173711_3174554_+|WP_044514727.1|DBSCAN-SWA
MSYIKAGPDANGHPVKLHYTDLGQGAPIVLIHGWPLNHESWEYQLKELPKHGVRVIAYTRRGFGNSDKPWDGYDYDTLADDLKAVLDELNLQNVTLVGFSMAGGEVARYMSRHGGARVSRVAFVSAVTPFLLKTADNPDGAPESAFTGIFEGLEKDRPDFMRSFAKKFYGVGLISHPVSDATLDWNQSLVMLGSPRATEACAHSFAETDFRQDLKTIKVPTLVIHGDQDETVPIEVSGARMQEFVPHATYKVYKGAPHGLFITEKDKLNEDIMAFAAGKA
>NZ_CP010054|3172257:3179100|3172257_3173322_-|WP_044514725.1|DBSCAN-SWA
MQAPKFAASRSFHQELKRRTNAYFAEAGTAPTGNKSLYFKAILLTASFVAVYLHIVFLTPPAWVAVLECALLGGIGAAIGFNVMHDGAHGSFSKSKWLNQFAAFTLNVMGGSSFMWNAKHNLIHHMYTNVEGVDDDLNAQPWLRLSPDQPRRMLHRFQHLYFWFFYALLFIAWIFYMDYQKYFQRKIGEMPIKKMTATDEGVFWGFKVLHLGLFVALPIYMVGFLPWLIGFVVFACVAGFSLSIVFQLAHTVEHTSFVVPHATTNKIEDEWAIHQIKTTANFATDSKIISWLVGGLNFQVEHHLFPTISHVHYPALNKILRQMCLEFGIEYNEYPNMRYAVASHVAHLRQLGRR

Homologous phage analysis in the prophage region

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_044514725.1|3172257_3173322_-	acyl-CoA desaturase	A6N231	Microbacterium_phage	1.9e-07	24.9
WP_044514727.1|3173711_3174554_+	alpha/beta hydrolase	A0A1D8EVD1	Mycobacterium_phage	4.0e-08	25.9
WP_044517148.1|3174718_3175213_-	HNH endonuclease	H6WG01	Cyanophage	1.1e-23	35.2
WP_044514729.1|3175539_3175959_+	hypothetical protein	NA	NA	NA	NA
WP_048826433.1|3176085_3176859_-	C40 family peptidase	A0A2H5BMT3	Streptomyces_phage	8.7e-10	39.8
WP_044514736.1|3177048_3177522_-	SsrA-binding protein SmpB	W5RAM5	Staphylococcus_phage	2.6e-25	45.7
WP_044514738.1|3177612_3178017_-	hypothetical protein	NA	NA	NA	NA
WP_044514741.1|3178089_3179100_-|tRNA	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD	A0A0R6PI74	Moraxella_phage	2.5e-65	42.7