CRISPRimmunity

Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Self targeting spacer number	Target MGE spacer number	Prophage number	Anti-CRISPR protein number
NZ_AP012327	Bifidobacterium catenulatum subsp. kashiwanohense JCM 15439 = DSM 21854 strain JCM 15439	5 crisprs	cas3,csa3,DEDDh,WYL	0	6	0	0

1. NZ_AP012327

CRISPR-Cas detection and classification

Crispr_ID: NZ_AP012327_1

• CRISPR_ID: NZ_AP012327_1
• CRISPR_location: 276934-277058
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 2 spacers

Consensus repeat of NZ_AP012327_1

Consensus_repeat	Method
TGTCCGTTTCTGCAGAATCGGAC	CRISPRCasFinder

spacers of NZ_AP012327_1

>1.1|276957|28|NZ_AP012327|CRISPRCasFinder
ATATGGAAGCCCATTTTGGGTGCGTACG
>1.2|277008|28|NZ_AP012327|CRISPRCasFinder
GTTCGCAGGCTGGTTTTGGGTGTGTGGC

CRISPR arrays and Neighbor proteins around NZ_AP012327_1

CRISPR arrays

The CRISPR arrays of NZ_AP012327_1

>merge|NZ_AP012327|1|276934-277058|CRISPRCasFinder
TGTCCGATTCTGGAGATTCGGACATATGGAAGCCCATTTTGGGTGCGTACGTGTCCGTTTCTGCAGAATCGGACGTTCGCAGGCTGGTTTTGGGTGTGTGGCTGTCCGTTTCGGCAGAATCGGAC

>NZ_AP012327|1|1|276934-277058|CRISPRCasFinder
TGTCCGATTCTGGAGATTCGGAC	ATATGGAAGCCCATTTTGGGTGCGTACG
TGTCCGTTTCTGCAGAATCGGAC	GTTCGCAGGCTGGTTTTGGGTGTGTGGC
TGTCCGTTTCGGCAGAATCGGAC

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
NZ_AP012327.1|WP_033500718.1|272987_274178_+|pyridoxal-phosphate-dependent-aminotransferase	unknown	unknown	gnl|CDD|180720
NZ_AP012327.1|WP_033500716.1|270777_271983_+|DUF2974-domain-containing-protein	unknown	unknown	gnl|CDD|371412
NZ_AP012327.1|WP_033500723.1|277910_278645_+|FadR-family-transcriptional-regulator	unknown	unknown	gnl|CDD|225097
NZ_AP012327.1|WP_081962248.1|274459_275425_-|decarboxylating-6-phosphogluconate-dehydrogenase	unknown	unknown	gnl|CDD|236582
NZ_AP012327.1|WP_033500715.1|269551_270778_+|DUF2974-domain-containing-protein	unknown	unknown	gnl|CDD|371412
NZ_AP012327.1|WP_033500724.1|279287_279611_+|translation-repressor-RelB	unknown	unknown	gnl|CDD|274102
NZ_AP012327.1|WP_033500711.1|265172_266036_+|ABC-transporter-permease-subunit	unknown	unknown	gnl|CDD|224098
NZ_AP012327.1|WP_033500726.1|283045_285784_+|ATP-dependent-DNA-helicase-RecG	unknown	unknown	gnl|CDD|236794
NZ_AP012327.1|WP_128832451.1|280147_280636_+|MerR-family-transcriptional-regulator	unknown	unknown	gnl|CDD|131585
NZ_AP012327.1|WP_033500714.1|268540_269326_-|mechanosensitive-ion-channel	unknown	unknown	gnl|CDD|366370
NZ_AP012327.1|WP_033500725.1|279600_279990_+|hypothetical-protein	unknown	unknown	unknown
NZ_AP012327.1|WP_033500722.1|277082_277634_-|gluconokinase	unknown	unknown	gnl|CDD|225804
NZ_AP012327.1|WP_033500721.1|275567_276896_-|gluconate-transporter	unknown	unknown	gnl|CDD|308194
NZ_AP012327.1|WP_003807649.1|282483_282678_+|50S-ribosomal-protein-L28	unknown	unknown	gnl|CDD|234736
NZ_AP012327.1|WP_033500713.1|267344_268445_+|CPBP-family-intramembrane-metalloprotease	unknown	unknown	gnl|CDD|376806
NZ_AP012327.1|WP_081962249.1|278839_279028_-|Vacuole-effluxer-Atg22-like-protein	unknown	unknown	gnl|CDD|341035
NZ_AP012327.1|WP_033500717.1|272151_272826_+|TetR/AcrR-family-transcriptional-regulator	unknown	unknown	gnl|CDD|224228
NZ_AP012327.1|WP_033500808.1|280810_282067_-|MFS-transporter	unknown	unknown	gnl|CDD|341035
NZ_AP012327.1|WP_033500727.1|285846_286449_+|16S-rRNA-(guanine(966)-N(2))-methyltransferase-RsmD	unknown	unknown	gnl|CDD|223813
NZ_AP012327.1|WP_033500712.1|266032_267214_+|ABC-transporter-ATP-binding-protein	unknown	unknown	gnl|CDD|226361

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
NZ_AP012327.1|WP_033500718.1|272987_274178_+|pyridoxal-phosphate-dependent-aminotransferase	gnl|CDD|180720	PRK06836, PRK06836, pyridoxal phosphate-dependent aminotransferase.	0
NZ_AP012327.1|WP_033500716.1|270777_271983_+|DUF2974-domain-containing-protein	gnl|CDD|371412	pfam11187, DUF2974, Protein of unknown function (DUF2974). This bacterial family of proteins has no known function.	1.76128e-125
NZ_AP012327.1|WP_033500723.1|277910_278645_+|FadR-family-transcriptional-regulator	gnl|CDD|225097	COG2186, FadR, Transcriptional regulators [Transcription].	6.98733e-31
NZ_AP012327.1|WP_081962248.1|274459_275425_-|decarboxylating-6-phosphogluconate-dehydrogenase	gnl|CDD|236582	PRK09599, PRK09599, NADP-dependent phosphogluconate dehydrogenase.	1.35026e-173
NZ_AP012327.1|WP_033500715.1|269551_270778_+|DUF2974-domain-containing-protein	gnl|CDD|371412	pfam11187, DUF2974, Protein of unknown function (DUF2974). This bacterial family of proteins has no known function.	4.50274e-90
NZ_AP012327.1|WP_033500724.1|279287_279611_+|translation-repressor-RelB	gnl|CDD|274102	TIGR02384, Putative_antitoxin_RelB, addiction module antitoxin, RelB/DinJ family. Plasmids may be maintained stably in bacterial populations through the action of addiction modules, in which a toxin and antidote are encoded in a cassette on the plasmid. In any daughter cell that lacks the plasmid, the toxin persists and is lethal after the antidote protein is depleted. Toxin/antitoxin pairs are also found on main chromosomes, and likely represent selfish DNA. Sequences in the seed for this alignment all were found adjacent to toxin genes. The resulting model appears to describe a narrower set of proteins than pfam04221, although many in the scope of this model are not obviously paired with toxin proteins. Several toxin/antitoxin pairs may occur in a single species. [Cellular processes, Toxin production and resistance, Mobile and extrachromosomal element functions, Other].	3.99999e-10
NZ_AP012327.1|WP_033500711.1|265172_266036_+|ABC-transporter-permease-subunit	gnl|CDD|224098	COG1177, PotC, ABC-type spermidine/putrescine transport system, permease component II [Amino acid transport and metabolism].	1.96188e-24
NZ_AP012327.1|WP_033500726.1|283045_285784_+|ATP-dependent-DNA-helicase-RecG	gnl|CDD|236794	PRK10917, PRK10917, ATP-dependent DNA helicase RecG; Provisional.	1.57949e-127
NZ_AP012327.1|WP_128832451.1|280147_280636_+|MerR-family-transcriptional-regulator	gnl|CDD|131585	TIGR02533, Type_II_secretion_system_protein_E, type II secretion system protein E. This family describes GspE, the E protein of the type II secretion system, also called the main terminal branch of the general secretion pathway. This model separates GspE from the PilB protein of type IV pilin biosynthesis. [Protein fate, Protein and peptide secretion and trafficking, Cellular processes, Pathogenesis].	0.000296498
NZ_AP012327.1|WP_033500714.1|268540_269326_-|mechanosensitive-ion-channel	gnl|CDD|366370	pfam00924, MS_channel, Mechanosensitive ion channel. Two members of this protein family of M. jannaschii have been functionally characterized. Both proteins form mechanosensitive (MS) ion channels upon reconstitution into liposomes and functional examination by the patch-clamp technique. Therefore this family are likely to also be MS channel proteins.	2.87148e-29
NZ_AP012327.1|WP_033500722.1|277082_277634_-|gluconokinase	gnl|CDD|225804	COG3265, GntK, Gluconate kinase [Carbohydrate transport and metabolism].	5.38858e-77
NZ_AP012327.1|WP_033500721.1|275567_276896_-|gluconate-transporter	gnl|CDD|308194	pfam02447, GntP_permease, GntP family permease. This is a family of integral membrane permeases that are involved in gluconate uptake. E. coli contains several members of this family including GntU, a low affinity transporter, and GntT, a high affinity transporter.	1.96829e-126
NZ_AP012327.1|WP_003807649.1|282483_282678_+|50S-ribosomal-protein-L28	gnl|CDD|234736	PRK00359, rpmB, 50S ribosomal protein L28; Reviewed.	7.51845e-22
NZ_AP012327.1|WP_033500713.1|267344_268445_+|CPBP-family-intramembrane-metalloprotease	gnl|CDD|376806	pfam02517, Abi, CAAX protease self-immunity. Members of this family are probably proteases (after a isoprenyl group is attached to the Cys residue in the C-terminal CAAX motif of a protein to attach it to the membrane, the AAX tripeptide being removed by one of the CAAX prenyl proteases). The family contains the CAAX prenyl protease. The proteins contain a highly conserved Glu-Glu motif at the amino end of the alignment. The alignment also contains two histidine residues that may be involved in zinc binding. While they are involved in membrane anchoring of proteins in eukaryotes, little is known about their function in prokaryotes. In some known bacteriocin loci, Abi genes have been found downstream of bacteriocin structural genes where they are probably involved in self-immunity. Investigation of the bacteriocin-like loci in the Gram positive bacteria locus from Lactobacillus sakei 23K confirmed that the bacteriocin-like genes (sak23Kalphabeta) exhibited antimicrobial activity when expressed in a heterologous host and that the associated Abi gene (sak23Ki) conferred immunity against the cognate bacteriocin. Interestingly, the immunity genes from three similar systems conferred a high degree of cross-immunity against each other's bacteriocins, suggesting the recognition of a common receptor. Site-directed mutagenesis demonstrated that the conserved motifs constituting the putative proteolytic active site of the Abi proteins are essential for the immunity function of Sak23Ki - thus a new concept in self-immunity.	1.02833e-14
NZ_AP012327.1|WP_081962249.1|278839_279028_-|Vacuole-effluxer-Atg22-like-protein	gnl|CDD|341035	cd17482, MFS_YxiO_like, Bacillus subtilis YxiO, Listeria monocytogenes BtlA, and similar transporters of the Major Facilitator Superfamily. This family is composed of Bacillus subtilis MFS-type transporter YxiO, and similar proteins including Listeria monocytogenes BtlA. The function of B. subtilis YxiO is still unknown, and L. monocytogenes BtlA is a putative secondary transporter involved in bile tolerance and general stress resistance. This family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.	2.07221e-05
NZ_AP012327.1|WP_033500717.1|272151_272826_+|TetR/AcrR-family-transcriptional-regulator	gnl|CDD|224228	COG1309, AcrR, Transcriptional regulator [Transcription].	2.03659e-08
NZ_AP012327.1|WP_033500808.1|280810_282067_-|MFS-transporter	gnl|CDD|341035	cd17482, MFS_YxiO_like, Bacillus subtilis YxiO, Listeria monocytogenes BtlA, and similar transporters of the Major Facilitator Superfamily. This family is composed of Bacillus subtilis MFS-type transporter YxiO, and similar proteins including Listeria monocytogenes BtlA. The function of B. subtilis YxiO is still unknown, and L. monocytogenes BtlA is a putative secondary transporter involved in bile tolerance and general stress resistance. This family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.	4.48119e-123
NZ_AP012327.1|WP_033500727.1|285846_286449_+|16S-rRNA-(guanine(966)-N(2))-methyltransferase-RsmD	gnl|CDD|223813	COG0742, COG0742, N6-adenine-specific methylase [DNA replication, recombination, and repair].	2.04462e-61
NZ_AP012327.1|WP_033500712.1|266032_267214_+|ABC-transporter-ATP-binding-protein	gnl|CDD|226361	COG3842, PotA, ABC-type spermidine/putrescine transport systems, ATPase components [Amino acid transport and metabolism].	1.51067e-132

Protein sequences

>NZ_AP012327.1|WP_033500721.1|275567_276896_-|gluconate-transporter
MNLIAAAIIGIAIIVLLIAVCKVHPFLSLLAGSFTMAVCAGVEYDEAFGSFTSGVGSTIANVGLLIAFGSIIGTILFKSGGADTIVDTIMAKTPLQRLPWAMALIAFIVGIPMFFEVGVVILIPVVLFAARRAKAPVVLLGIPALAGLSTLHAFVPPHPGPLTAIGALNANLGITLALGLLVAIPTVIISGPIFGKLAAKWVPITAPENKAEAEAPKSAENRPSFASAIAVILLPVVLMLAASIVDLTGQSETTWGRALAFIGTPLTALLITTVFAMVALGYLQKFSRDAVNGMVGQSFSSVAGIILIVAAGGGFKQTLVDSGIGDVIANSITESAMNPLIAGWLVAVLIRLATGSATVATVTASGIMVPLATGMSPTHLALLVLAIGAGSVFFSHLNDAGFWLVKEYFGMTVGQTLKTWSLMETILSVTGLGCVMLLSLVL
>NZ_AP012327.1|WP_081962248.1|274459_275425_-|decarboxylating-6-phosphogluconate-dehydrogenase
MYGRHQSQSNPQFHIKHNRRKKNIMQLAMIGLGRMGGNMAKRITAAGHEVVGYDRSPESDRTVASLEEMVTALTAPRIVWVMVPAGEATDSTINELAELLEPGDMIIDGGNSRYTDDARHAAELEPKGIHFMDCGVSGGVWGIDRGYALMVGGSEEDFERARPIFEALKPEGDSGLVLAGPVGGGHFAKMVHNGIEYGMMQAFGEGFATMVKSDLIKDPAAVMSSWRDGSVVQSWLLDLLAIAFKSDPTLQSMPPVANESGEAKWMIEAALELGVPTPATAAALYARQTSRGGADDILRVVSTMRAQFGGHVTKIDEIATH
>NZ_AP012327.1|WP_033500718.1|272987_274178_+|pyridoxal-phosphate-dependent-aminotransferase
MINEAYKAMLGGKSVIRELSEYATVRGAEIGYENVFDYSLGNPSVPCPQSFTDEMIALYQGVEPVALHGYSPSLGIASVRAAIAESLNRRFDMDYTADYIFPTTGAAGALAHALRVVTKPGDEVITFAPYFPEYQPYVNGTGAHLTVVPADTESFQINFEAFEQVLNPGTAAVLINTPNNPSGAVYSAETLTRLAEVLRAKQVEYGHDIFLISDEPYREIVFDGGVQPYPSKFYDNTLSCYSYSKSLSLPGERIGYVAVNPRATDAELIVPMCGQVSRETGHNCPGSSVQLAVAKVVDETSDLSVYEKNMNLLYDALVRLGFDVVRPGGTFYIFPKALEEDANAFCMKAKDYDLILVPSDSFGVPGYFRMAYCINTEKVERSIAALEKFVHEVYGR
>NZ_AP012327.1|WP_033500717.1|272151_272826_+|TetR/AcrR-family-transcriptional-regulator
MPRPRRDSEILPAKERLENAFWELLSEREYNKITVTDIVRAADVNRNSFYYHFSGLPELADSAILHAVENTPMPGVPGRDFDPDVEWRKHVTALLRDPEQRQRLDRLALLAGPHSSPELVSSLKEFGRLTMISVLGLNADNLDLKTDLMLDFTVGGMLSVLQRWPKLHEKLPIDTVFNEDVAVLAAGMFMSMSKADMLEYWRRIFSEGFVPSMAISQQQPPSAK
>NZ_AP012327.1|WP_033500716.1|270777_271983_+|DUF2974-domain-containing-protein
MGNIIDYARTETRSFEALPFREADALVLAQLSYDEVPKCVLLLDDLVAKYGTLQARAKQFDIRRPIASLRMLRTPPFGGATIARADDELNHDKPVADHDVENVGLVDPQVTHDFYHAVAANPRFSDVEMSAYCEQFDGDAQTQFAAVTFRLPSGTLVVAFRGTDDSLVGWKEDFNMAFQYPVPAQVSAAEYLKKVASLWDGPILLTGHSKGGNLAVYAAMNAEDEIKDRVERIYSLDGPGFPEEVVKSFEYASVSDRIVKIVPDSSVVGMVFETPEHCVVVKSDVDGIMQHFAFSWQMHGGEFAKAEDVADSSVVFNKSLNGWLAGLSKEQREHAVDALFSVLEASGAGSISAIVAAGPKVIPEMLGTYVGLSSADRRNINQALVILLQAALARNPKVQRK
>NZ_AP012327.1|WP_033500715.1|269551_270778_+|DUF2974-domain-containing-protein
MGNVVDYVRREFRGFGELAFSNVDSLVLSELSYMRLLGLVPVFGEAKSVATVPIRELLRAESYDEMFVSNSSEMNDYRLSLLRAVCESPRFRGLRVGEYAERLDEGEQQQFAAMTFDLGADFGLYVAFRGTDGTLVGWKEDFNMAVRCPVPSQESAYRYADSILDRTERLLSAKESPGIMIGGHSKGGNMAVYAAMQITQSDIAATSERAQRLGLLPALGGSVPGRNCRISRIFSHDGPGMSQVMVHSRAYQAIAARIDKTVPESSIIGMLLQSQIKPTLVKADAISILQHMGSSWQVTQSGEFERASELTSGAQLIGKTIDGWFDRVSQEQRERAINQMYDIFAAAGYGNIADLAANWTDSLPKIVAAARGTDVETRELIKDVLKAIPASAAKVAVGELRNDKEGDA
>NZ_AP012327.1|WP_033500714.1|268540_269326_-|mechanosensitive-ion-channel
MEPIEIAVDWLKTNSGKIILLIVVALLAAVIDKALTRIVSKVLDRSQVPNASIFINILRAVVWTLAVGTVLQPVFGINPTTIFTALGIGGLAVSLGMKDTIANIIGGFGLMLGRVIQPGDLVNVAGTDGVVEDINWRQTVVRERNGNVKIIPNSILNTTSLEKLNPSNEMLVRVPFTAKAGTDIDEMTRQVVQAVQRDTADLADPRFPPKVRLTGYSPYGINVEVCAYAKPGSLLPDMINAVARSIANADFLENRAINGES
>NZ_AP012327.1|WP_033500713.1|267344_268445_+|CPBP-family-intramembrane-metalloprotease
MTFPQYPQYPQRPQQQNPQQPFQYQQQPQYPRPQVNPYERWLRRVKSVFSRIGVGMCLMVVIWYALATVISGVLYQVLHTTNLPNWAVYVASDAPLYLVAMPLTVLIMGKSSVIETRKFDMKPGQFFKLLVMCFPLMYVGSLIGNMLASLLSGGKASNSVSDLAMQFDVWNVVFLVILGPLFEEWIFRKELISRTRKYGEKTAIVFSAMFFGLVHMNLFQFFYAFALGLVFGYVYVRTSKLRYSVVMHMCVNFMGGVVAPWVVTNIDIDSLMNVLTSAENGDGTAMMQWMGQNATGTMIFGIYMLLYYGLIIAGVVLLIRERKNFVFYTAPEELPRGVRTRTAILNVGVITYIVVTVILTAINLMR
>NZ_AP012327.1|WP_033500712.1|266032_267214_+|ABC-transporter-ATP-binding-protein
MTLADQKATIARTSEAFVDADLSAETVTRAAREVLNDSAERGGGVQMLNVDKIYPGATNKALDNFNLEINPGEMVVLLGGSGCGKSTALRSLAGLEDIQAGRILVGGQDVTGVPANKRNMAMVFQAYSLFPHMTALQNVEFGLEIRGMGKTERRKQAMEKLELVGLADQAGKYTQQMSGGQQQRVALARALAVNPRVLLLDEPLSALDAKVRVQLRDEIRRIQLEAGTTTVFVTHDQEEALAVADRIGVMNHGCIEQIADPQTLYRRPASEYVATFIGLTNRLPGITNGEEAVVFGQRVPLLEASKRDASTVLVRPENLVITLAANDGNMVGERGRVDMVHFLGALVRVDLTVAAGAGHLPVTVQLPANELPAGLTVGSEVVVAPRPIAALAV
>NZ_AP012327.1|WP_033500711.1|265172_266036_+|ABC-transporter-permease-subunit
MAVNHTKTMPPQLRAARVRKQRVIKAIILTFTLLFLFVPLVSMFVFTIRHPLSGKWSLDPWIAIFTGDGSALGADLSTLWNGLGSSMGLCVVTVVIMLLLMVPTMVIVHIRSKQLERAIEWVATLPLTIPAIVLVVGLGPIYRWLSADVLNTNPIWLCFAYVILVMPFAFRALAVGLNSIDVKTLCEASRSLGASWPRVFFRVLIPNLWQSILSASFISIAVVLGEYTVASLLGRMNLQVALYQLGQSNSQISTAMSLLALLFGVILLVALDLISDALRKSKESNES
>NZ_AP012327.1|WP_033500722.1|277082_277634_-|gluconokinase
MSIHVVVMGVAGCGKSTVAEAIHERLGYVYAEGDDFHPQANIDKMSAGIPLTDEDRWPWLNVINSWMVAREALDENTVVSSSALKRSYREVLAKDVPTFFIHLNGSHELIQQRLSERKGHFMPPALLPSQFAILEPLAPEENGVEISIEGSVDEMVDRAIEALNAYAATVAATVTAQTATQAA
>NZ_AP012327.1|WP_033500723.1|277910_278645_+|FadR-family-transcriptional-regulator
MAEGTVIAENAGGELMQDSVSRTLAIEMLDGEWKAGESLTLEALQSRFGISRTVAREVAKTLESMNAVLVKRRIGLVARPFGEWQALNHQVIEWRLHSAQREQQLSSLTELRLAVEPAAAASAARLAPIDVKAKFPVYAAQMRQIAEANEEGEAGLAQFHDLDVEFHTLILHESGNELFAALSDTIATVLRGRVELGKYPMKPKSAALDAHDAVADAIAKGEPERARSAMLDIVDEVARALNFF
>NZ_AP012327.1|WP_081962249.1|278839_279028_-|Vacuole-effluxer-Atg22-like-protein
MISRVPRSHYCFFPKTASTIGTSLVATTTSLTGNASVGVLSIAILLIVALIFLLPQKDPTRK
>NZ_AP012327.1|WP_033500724.1|279287_279611_+|translation-repressor-RelB
MSTTTVRMDDDLKAEVNAILDSMGLNFNTFVNMASVQLVSQRRIPFEVKAPEPVLPRVGRVAANGVTYRGVDEQGYPVVEVPNAMVLNPSRGADGVAVLPKAWRDGE
>NZ_AP012327.1|WP_033500725.1|279600_279990_+|hypothetical-protein
MANNHDIPELMPVPEQLHVFDVYRMQVRFEDDPTKSRNHYVAAIVIQADDDGGVAVKLTSNPTWHGAGDVQLPEYEHAGLTHLTTARCAQLVRFLRSDLQGFTGRLSRNDAIRVANAVGEVKPEEQVWL
>NZ_AP012327.1|WP_128832451.1|280147_280636_+|MerR-family-transcriptional-regulator
MVDLLDQQTASLQSGIAEQKQRLDDCLQLRKALTVSASDFVSSTLTDMATVMNTTTTHSLRTTYFVMLAIALPATLLQIACLVIGVMTDVWWPLPVAVLLAIALTVAAMKYYRSRVQYLCPACHETFQPGMREFVFAAHTPKTRKLTCPHCGHRGYCMELSI
>NZ_AP012327.1|WP_033500808.1|280810_282067_-|MFS-transporter
MLKPNAKLTTREKKWIVYDVGNSAFVMLSTAVVPIYANSLLQSAGQSNIVSTWGYAQTIASLIIAVMMPVLGSMADVQGMKVKFFTGFFLTGVVACCAMALPLSWLVFLVVCILATVGLNGSLTFYDSMLVDITSNERMDRVSSHGFAWGYIGSTIPFIACIALIFGGPSLFGWSTVACTRASFVITALWWVAFTIPLLTSYKQVHYRATAGQTGEAIRGTLAELGSTFRAIRKNKPLWMFMIAFFFYIDAVNTVISMSTSYGTQLGIDSTHLVMALLVTQFVAFPSAIAYGKLAGHFGAKTMIATAVIAYICIVLFAAFFLRSATEFWILAILVGLFQGGIQALSRSYYGKIIPKNRANEYYGFYDIFGKTASIIGTFLVATTTSLTGNASVGVLSIAILLIVALIFLLLQKDPTQA
>NZ_AP012327.1|WP_003807649.1|282483_282678_+|50S-ribosomal-protein-L28
MAARCAVCGKGPQTGFTVSHSHIRNKRTFRPNLQPVRTTIDGENVRVRVCVKCIKAGKVQRVEA
>NZ_AP012327.1|WP_033500726.1|283045_285784_+|ATP-dependent-DNA-helicase-RecG
MSTTLETPISSIESNRRRVGALKSLGVVSVGDALTYYPFRVTDPVPAHALHEAKIGEKMAFAAHVLETRVFPMARRGFRLIATVTDDDFAARRNTPKSLASLVFFSYRKSYVDWVQRKLHTGALLVVAGEPSVYDNRLQFTHPDLLTINPVQSQAESGEWNDGFGDPANPPLGNLKYDAQTVDEALKRVCRPRPVYHANSRISSEHIHESVLKYMDALRGSEYLPSQTPNNSSDNIAQEGEFDAPQVDREVQIKSLSVAIPDIIPEDFREQYGLMHRAEAFMAIHDPVDRKNFDNALQTLRYEEALICQTALVKARDASRKNKATACSVTRLKDDFIASLPFALTSGQRQVIADISADMTRDYPMQRLLQGEVGSGKTVVAVAAMMQAVGSGRQAVLVAPTQVLAEQHYASISKMVAKLTDADDKTASEIRENTAPTKASAIQQNGESAQQTINNNAVDKGAQLADLLDIAEISNVDNSNNDNSITLRSEQSHISDAQKVGIPSKGNDIFGTKDGEIPVFLLTGGMRLAERRRVLAAAASGMPCIVVATHAAFSKSFQAPNLTLAVIDEQHRFGVEQRESLNSKGATAPHLLVMTATPIPRTAAMTWFGDLDISALTELPGGRKPIRTFVVPESNASLMGEMFALIRKRIDAGERAYVVCPRIDDDSENADDVLAESAASSSKTTGSPVPAFDEAYDLGEDDEQRAQRPPLHSVAEIVERLQSLPQFKGIRFATLTGRDDDTTKSQVMADFEAGITPILVATTVIEVGVDVAKASCIVIFDADRYGLSQLHQLRGRVGRGGTDSGAFLISRAPADSDAARRLDVIQGTLDGAEIAQADLEFRGAGDVLGDAQSGGKTGLKLLRVVKDMKIIEQARAEATQLVAADPDLLGFVQLAGAVLDFTRGNEMFLTSN
>NZ_AP012327.1|WP_033500727.1|285846_286449_+|16S-rRNA-(guanine(966)-N(2))-methyltransferase-RsmD
MRVISGRFKGVALTTPKSGTRPTTDRTKEAIFSHLDSWGVLDDARVLDLFAGTGALGIEALSRGARELVAVESSAPAAALIAQTLTALKHNRSWEHGMSARVVKARAEKYAASASAVEPFDMVFIDPPYAFETNDCNQLLADLASRGLTSSNTVIMLERSTRSEAPTAPANWEITDRRDYGETAVYYIEPSEPISDNDQQ

Crispr_ID: NZ_AP012327_2

• CRISPR_ID: NZ_AP012327_2
• CRISPR_location: 360478-360781
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 6 spacers

Consensus repeat of NZ_AP012327_2

Consensus_repeat	Method
GTCGTTCCGATCGTGACGANCG	CRT

spacers of NZ_AP012327_2

>2.1|360500|29|NZ_AP012327|CRT
TCGCGGCAGCCGTGGCCGTCGTGATGATC
>2.2|360551|26|NZ_AP012327|CRT
TGATTATGATGATCGTCCGCGTCGTC
>2.3|360599|29|NZ_AP012327|CRT
CCGCGACTATGATGATCGTCCGCGTCGTC
>2.4|360650|20|NZ_AP012327|CRT
TGATTACGATCGTGATGATC
>2.5|360692|26|NZ_AP012327|CRT
TGATTATGATGATCGTCCGCGTCGTC
>2.6|360740|20|NZ_AP012327|CRT
TGATTACGATCGTGATGATC

CRISPR arrays and Neighbor proteins around NZ_AP012327_2

CRISPR arrays

The CRISPR arrays of NZ_AP012327_2

>merge|NZ_AP012327|2|360478-360781|CRT
AGCGTTTCGATCGCGATGACCGTCGCGGCAGCCGTGGCCGTCGTGATGATCGTCGTTCCGATCGTGACGATCGTGATTATGATGATCGTCCGCGTCGTCGTCGTTCTGATCGTGACGATGACCGCGACTATGATGATCGTCCGCGTCGTCGTCGTTCCGATCGTGACGACCGTGATTACGATCGTGATGATCGTCGTTCCGATCGTGACGATCGTGATTATGATGATCGTCCGCGTCGTCGTCGTTCCGATCGTGACGACCGTGATTACGATCGTGATGATCGTCGTTCCGACCGTCGCCGCAG

>NZ_AP012327|2|1|360478-360781|CRT
AGCGTTTCGATCGCGATGACCG	TCGCGGCAGCCGTGGCCGTCGTGATGATC
GTCGTTCCGATCGTGACGATCG	TGATTATGATGATCGTCCGCGTCGTC
GTCGTTCTGATCGTGACGATGA	CCGCGACTATGATGATCGTCCGCGTCGTC
GTCGTTCCGATCGTGACGACCG	TGATTACGATCGTGATGATC
GTCGTTCCGATCGTGACGATCG	TGATTATGATGATCGTCCGCGTCGTC
GTCGTTCCGATCGTGACGACCG	TGATTACGATCGTGATGATC
GTCGTTCCGACCGTCGCCGCAG

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
NZ_AP012327.1|WP_033500774.1|372505_373846_+|C1-family-peptidase	unknown	unknown	gnl|CDD|238328
NZ_AP012327.1|WP_004220095.1|361090_361651_+|LemA-family-protein	unknown	unknown	gnl|CDD|377192
NZ_AP012327.1|WP_033500764.1|354811_356629_+|amylosucrase	unknown	unknown	gnl|CDD|200463
NZ_AP012327.1|WP_003836102.1|356942_357617_+|hypothetical-protein	unknown	unknown	unknown
NZ_AP012327.1|WP_033500759.1|336942_339042_+|membrane-protein	unknown	unknown	unknown
NZ_AP012327.1|WP_033500760.1|340040_340622_-|biotin-transporter-BioY	unknown	unknown	gnl|CDD|376871
NZ_AP012327.1|WP_033500767.1|364074_365481_+|AAA-family-ATPase	unknown	unknown	gnl|CDD|224586
NZ_AP012327.1|WP_033500762.1|344538_353868_+|type-I-polyketide-synthase	unknown	unknown	gnl|CDD|227315
NZ_AP012327.1|WP_003807806.1|357749_358019_+|30S-ribosomal-protein-S15	unknown	unknown	gnl|CDD|180170
NZ_AP012327.1|WP_081962251.1|339104_340016_+|biotin--[acetyl-CoA-carboxylase]-ligase	unknown	unknown	gnl|CDD|223417
NZ_AP012327.1|WP_033500771.1|369326_370154_+|2-C-methyl-D-erythritol-4-phosphate-cytidylyltransferase	unknown	unknown	gnl|CDD|133009
NZ_AP012327.1|WP_033500761.1|342758_344441_+|acyl-CoA-carboxylase-subunit-beta	unknown	unknown	gnl|CDD|227136
NZ_AP012327.1|WP_033500770.1|368222_369239_+|laccase-domain-containing-protein	unknown	unknown	gnl|CDD|376831
NZ_AP012327.1|WP_048349492.1|371034_372513_+|cell-division-protein	unknown	unknown	gnl|CDD|173412
NZ_AP012327.1|WP_033500769.1|366600_368139_+|U32-family-peptidase	unknown	unknown	gnl|CDD|223896
NZ_AP012327.1|WP_033500816.1|340863_342759_+|ATP-grasp-domain-containing-protein	unknown	unknown	gnl|CDD|227111
NZ_AP012327.1|WP_033500768.1|365574_366438_+|aldo/keto-reductase	unknown	unknown	gnl|CDD|381314
NZ_AP012327.1|WP_033500772.1|370196_370886_+|pyroglutamyl-peptidase-I	unknown	unknown	gnl|CDD|238279
NZ_AP012327.1|WP_033500766.1|361762_363976_+|DUF2207-domain-containing-protein	unknown	unknown	gnl|CDD|378333
NZ_AP012327.1|WP_082139057.1|354095_354614_+|4'-phosphopantetheinyl-transferase-superfamily-protein	unknown	unknown	gnl|CDD|234610

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
NZ_AP012327.1|WP_033500774.1|372505_373846_+|C1-family-peptidase	gnl|CDD|238328	cd00585, Peptidase_C1B, Peptidase C1B subfamily (MEROPS database nomenclature); composed of eukaryotic bleomycin hydrolases (BH) and bacterial aminopeptidases C (pepC). The proteins of this subfamily contain a large insert relative to the C1A peptidase (papain) subfamily. BH is a cysteine peptidase that detoxifies bleomycin by hydrolysis of an amide group. It acts as a carboxypeptidase on its C-terminus to convert itself into an aminopeptidase and peptide ligase. BH is found in all tissues in mammals as well as in many other eukaryotes. Bleomycin, a glycopeptide derived from the fungus Streptomyces verticullus, is an effective anticancer drug due to its ability to induce DNA strand breaks. Human BH is the major cause of tumor cell resistance to bleomycin chemotherapy, and is also genetically linked to Alzheimer's disease. In addition to its peptidase activity, the yeast BH (Gal6) binds DNA and acts as a repressor in the Gal4 regulatory system. BH forms a hexameric ring barrel structure with the active sites imbedded in the central channel. The bacterial homolog of BH, called pepC, is a cysteine aminopeptidase possessing broad specificity. Although its crystal structure has not been solved, biochemical analysis shows that pepC also forms a hexamer. .	0
NZ_AP012327.1|WP_004220095.1|361090_361651_+|LemA-family-protein	gnl|CDD|377192	pfam04011, LemA, LemA family. The members of this family are related to the LemA protein. LemA contains an amino terminal predicted transmembrane helix. It has been predicted that the small amino terminus is extracellular. The exact molecular function of this protein is uncertain.	4.30523e-80
NZ_AP012327.1|WP_033500764.1|354811_356629_+|amylosucrase	gnl|CDD|200463	cd11324, AmyAc_Amylosucrase, Alpha amylase catalytic domain found in Amylosucrase. Amylosucrase is a glucosyltransferase that catalyzes the transfer of a D-glucopyranosyl moiety from sucrose onto an acceptor molecule. When the acceptor is another saccharide, only alpha-1,4 linkages are produced. Unlike most amylopolysaccharide synthases, it does not require any alpha-D-glucosyl nucleoside diphosphate substrate. In the presence of glycogen it catalyzes the transfer of a D-glucose moiety onto a glycogen branch, but in its absence, it hydrolyzes sucrose and synthesizes polymers, smaller maltosaccharides, and sucrose isoforms. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase.	0
NZ_AP012327.1|WP_033500772.1|370196_370886_+|pyroglutamyl-peptidase-I	gnl|CDD|238279	cd00501, Peptidase_C15, Pyroglutamyl peptidase (PGP) type I, also known as pyrrolidone carboxyl peptidase (pcp) type I: Enzymes responsible for cleaving pyroglutamate (pGlu) from the N-terminal end of specialized proteins. The N-terminal pGlu protects these proteins from proteolysis by other proteases until the pGlu is removed by a PGP. PGPs are cysteine proteases with a Cys-His-Glu/Asp catalytic triad. Type I PGPs are found in a wide variety of prokaryotes and eukaryotes. It is not clear whether the functional form is a monomer, a homodimer, or a homotetramer.	4.10707e-52
NZ_AP012327.1|WP_033500760.1|340040_340622_-|biotin-transporter-BioY	gnl|CDD|376871	pfam02632, BioY, BioY family. A number of bacterial genes are involved in bioconversion of pimelate into dethiobiotin. BioY is a component of the BioMNY transport system involved in biotin uptake in prokaryotes.	4.95946e-28
NZ_AP012327.1|WP_033500767.1|364074_365481_+|AAA-family-ATPase	gnl|CDD|224586	COG1672, COG1672, Predicted ATPase (AAA+ superfamily) [General function prediction only].	3.39597e-41
NZ_AP012327.1|WP_033500762.1|344538_353868_+|type-I-polyketide-synthase	gnl|CDD|227315	COG4982, COG4982, 3-oxoacyl-[acyl-carrier protein].	0
NZ_AP012327.1|WP_003807806.1|357749_358019_+|30S-ribosomal-protein-S15	gnl|CDD|180170	PRK05626, rpsO, 30S ribosomal protein S15; Reviewed.	9.54707e-57
NZ_AP012327.1|WP_081962251.1|339104_340016_+|biotin--[acetyl-CoA-carboxylase]-ligase	gnl|CDD|223417	COG0340, BirA, Biotin-(acetyl-CoA carboxylase) ligase [Coenzyme metabolism].	2.45627e-36
NZ_AP012327.1|WP_033500771.1|369326_370154_+|2-C-methyl-D-erythritol-4-phosphate-cytidylyltransferase	gnl|CDD|133009	cd02516, CDP-ME_synthetase, CDP-ME synthetase is involved in mevalonate-independent isoprenoid production. 4-diphosphocytidyl-2-methyl-D-erythritol synthase (CDP-ME), also called 2C-methyl-d-erythritol 4-phosphate cytidylyltransferase catalyzes the third step in the alternative (non-mevalonate) pathway of Isopentenyl diphosphate (IPP) biosynthesis: the formation of 4-diphosphocytidyl-2C-methyl-D-erythritol from CTP and 2C-methyl-D-erythritol 4-phosphate. This mevalonate independent pathway that utilizes pyruvate and glyceraldehydes 3-phosphate as starting materials for production of IPP occurs in a variety of bacteria, archaea and plant cells, but is absent in mammals. Thus, CDP-ME synthetase is an attractive targets for the structure-based design of selective antibacterial, herbicidal and antimalarial drugs.	2.96816e-70
NZ_AP012327.1|WP_033500761.1|342758_344441_+|acyl-CoA-carboxylase-subunit-beta	gnl|CDD|227136	COG4799, COG4799, Acetyl-CoA carboxylase, carboxyltransferase component (subunits alpha and beta) [Lipid metabolism].	0
NZ_AP012327.1|WP_033500770.1|368222_369239_+|laccase-domain-containing-protein	gnl|CDD|376831	pfam02578, Cu-oxidase_4, Multi-copper polyphenol oxidoreductase laccase. Laccases are multi-copper oxidoreductases able to oxidize a wide variety of phenolic and non-phenolic compounds and are widely distributed among both prokaryotes and eukaryotes. There are two main active catalytic sites with conserved histidines that are capable of binding four copper atoms.	6.96823e-63
NZ_AP012327.1|WP_048349492.1|371034_372513_+|cell-division-protein	gnl|CDD|173412	PTZ00121, PTZ00121, MAEBL; Provisional.	1.08829e-06
NZ_AP012327.1|WP_033500769.1|366600_368139_+|U32-family-peptidase	gnl|CDD|223896	COG0826, COG0826, Collagenase and related proteases [Posttranslational modification, protein turnover, chaperones].	4.68728e-114
NZ_AP012327.1|WP_033500816.1|340863_342759_+|ATP-grasp-domain-containing-protein	gnl|CDD|227111	COG4770, COG4770, Acetyl/propionyl-CoA carboxylase, alpha subunit [Lipid metabolism].	0
NZ_AP012327.1|WP_033500768.1|365574_366438_+|aldo/keto-reductase	gnl|CDD|381314	cd19088, AKR_AKR13B1, AKR13B family of aldo-keto reductase (AKR). Xylella fastidiosa phenylacetaldehyde dehydrogenase is a founding member of aldo-keto reductase family 13 member B1 (AKR13B1). phenylacetaldehyde dehydrogenase (EC 1.2.1.39) catalyzes the NAD+-dependent oxidation of phenylactealdehyde to phenylacetic acid.	7.97027e-86
NZ_AP012327.1|WP_033500766.1|361762_363976_+|DUF2207-domain-containing-protein	gnl|CDD|378333	pfam09972, DUF2207, Predicted membrane protein (DUF2207). This domain, found in various hypothetical bacterial proteins, has no known function.	3.52697e-78
NZ_AP012327.1|WP_082139057.1|354095_354614_+|4'-phosphopantetheinyl-transferase-superfamily-protein	gnl|CDD|234610	PRK00070, acpS, 4'-phosphopantetheinyl transferase; Provisional.	3.24746e-21

Protein sequences

>NZ_AP012327.1|WP_003807806.1|357749_358019_+|30S-ribosomal-protein-S15
MALTAEEKQEIIKTYATHEGDTGSPEVQVALLTKRIADLTEHLKEHKHDHHSRRGLLLMVGDRRRMLDYLKRVDINRYRSLIERLGLRR
>NZ_AP012327.1|WP_003836102.1|356942_357617_+|hypothetical-protein
MAQTQGNKVTEVVDGVVEQVDVSKHPTASIEASDLSLADIERRKSHPGHWALFIALMLVALIVPYWWGRFLAVKDAAWIVANLGFLDPKGVALISWTVTIMAMTGLGLMVADVKKWLWGTVFVVGLAAEQFVAGMCLLSFNFWNATYVMYGDSSGLANAANLGIIAAGCGVAVYAVLWVGLLVCIKKESKFNVLTRSWASFLLFFVIEGISLAVVLFGGLLNVV
>NZ_AP012327.1|WP_033500764.1|354811_356629_+|amylosucrase
MVRSKSDQAFDERIAKNSEELRELLTDLYGEQAEEAFQNLTELMKASHAARPADLKRLDTKRLADPEWYKRGNMFGMTMYTDLFAGDLKKLAGKVPYLKERKLTYLHLMPLLKMPHPQNDGGYAVEDFDTVDPSLGTNEDLAALTKKLRAAGISLCLDFVMNHTASSHRWAKAAQAGDPKYQDYYYCYDDRTVPDQYDAVVPEVFPATAPGNFTWNEQMHKWVLTSFYPFQWDLNYRNPNVLVSVLSSVLGLANLGVEVFRIDAVPYIWKELGTNCRNLPQVHTIVRMLRIVLECVCPAVVLKGEVVMAPKELAAYFGTPEKPECHMLYNVSIMVNLWSALASGDVRLLKDQLDKLNALPENCWFVNYLRCHDDIGWGLDEPMEESLGIDPLKHKEFLYHFYEGSVPGSWAMGELYNYDEASKDARSCGTTASLCGVERALITHDKPLLDISMKRDLMMHSAMSFLRGFPMLSCGDEIVQLNGWEYKEDPDRVEDSRNLHRSPFNWENAAKRKQTGALQKQMWDGLKSVREMRDDPAFAPEAWVTTWDAHNDAVLAVVRHVEGRTVLGLFNFSSARQIARLDSDGDIVLPAEVALEPYQVCVVEK
>NZ_AP012327.1|WP_082139057.1|354095_354614_+|4'-phosphopantetheinyl-transferase-superfamily-protein
MLCGNRHGSFLPENFRKVGKCWVWGDDVVDVPVFAEQLGESGSRMRNLFSTRELWQTTQRARVKRDGEAVHLAARWAGKEAFLKAWCEAISCRAKGDGDIAYPYTLDDFPWARIEILDDSHGVPHVMLSPEVRCKLRQSLGLPVDGDGQSCEMHISISHDGAVASAVVTIEA
>NZ_AP012327.1|WP_033500762.1|344538_353868_+|type-I-polyketide-synthase
MNTFFLDRLNGEPHALAFAGQSTPWPVALTDQSANPVLNEALHAHADAAQTLLYPVSADLLATTGRPVDLFGFEPNPARLGAAAAASASVPGIALAQLGALLDAASLGYNPAVAKPVAVLGHSQGVLAVHMVQSIVEAGSIEAASAQIDEILAIATLIGVAGTRQARQLGLAARHGEATPMLSVKDITRAQVDALIKRVSGARGPIAVAVTNSATHYVLSGYPEDLTAFGVEVAKEHKHQAKLREEKVRGGRVFEPVLEYLDVTLPFHSPLMADAVSQAVSWAESCGIDADHARELAAEVLLNHVDWAARVRALMKSTDPSALWVLDFGPGTTVGKLFSTVAQGTGVGVVEASTVADRGELSTLETLPERTQNWTRFAPSIIHTSAGDKVRTAFTELTGKAPVLLAGMTPTTVEPEIVAAAANAGYWAELAGGGQVTASVFDRHVAKLEEELEEGRTVEFNAMFMDRYLWNLQFGSQRIVPKKRASGTPIDGVVVSAGIPELDEAVELIHNLNADGFPYVSFKPGTVDQIRQVVRIAKAVAPVKVLIEVEGGSAGGHHSWESLDDLLLSTYAEVREQSNLVLVVGGGIGTPERGADYITGEWSAEYGRPLMPVDGVLVGTAVMTAKEAHTSPEVKQMLVNTPGIPAKGADADPFAPLGEQWVPSGQAKGGVTSGLSHLHADIYELENSSARCGRLLVRVMKHPEELESRREEIIEALNATAKPYFGDLKAMTYLAWAQRFADLAFPWVDETYADRFLHLLQRIEARVTTQESGEFASLFASRADVESDPHAAIAKLAEAYPQADELTVTPMDEAWFPVLVREYPKPMPFVPVIDNDLLRWWGQDQLWQSEDSRYSADSVRIIPGPISVAGITTVDEPVADILGRFEAAMVKRVSAESPIAAKTAFAQLGVASSAEEFIRKSPNISWVGHLMANPAYGTSNGDANYEIRKISSEGSVEKYDLDIHLDTYWDNDPDGGKSKHAVRDIVIPLNVDGNESGLFPVVDRDRLPEHVYRMLADTAGIGNTAITGDKLTAMPVIEQVADKASYPFGQAHTSYTLSANLGFDHEAATGGALPSNLVPSRIAPDALVGPAWPAIYAALGSVYVNGFPVIEGLLNAVHLDHLIELEVSEDELLKHIGEQIALTSWAEDYFESASGRVVTIHVTHSTQDGTVLANETERFAIRGRAYSDALPPEAPDYGGIDAEIESTPRRLLRRVKVTAPHEMTAFARTSGDFNPIHTSHRGAAVSGLAAPLVHGMWLSATAQYAVQALDGKGAHYEIAGWTYNMYGMVQLDDEVEISIERVGRVAHSGMVLEVTSRIKGNIVSRGTAIVRAPKSAFVYPGQGIQRQGMVLDERAKSPAARDVWERADKVTREKLGFSILAVVRDNPKELTANGVTYRHPEGLLNLTQFTQVALATVAFAQTARLREAGADIWPSYFAGHSLGEYNALSSFAGVIPLETVIELVFHRGSTMHHLIPRDAKGRSNYRMGALRPNQFGVGDDGVREYVESVSKASGEFLQIVNYNLAGQQYAVAGTIAGLKALKADSSRRVAEYGGKPAFMLVPGIDVPFHSTLLRKGVPEFRDKLDALLPQTIDYRGRLVGRYIPNLVAAPFEMTKEFAAKILEVVPSERIQAALDDPQIWDSYAADDQKLGRLLLTELLSWQFASPVRWIETQALLFGSSEQGGLGVEEYVEVGLGNAPTLANLGSKTLRLPQFAGRDVTVYNVGRDEGRVYMTDSDSLVPEEDADDSAVVTSASSSAAPAVASAPAAAPAAVAEVPVVAALAGAPSDVAVADIAFRASDAIATLLAYSAKVRPDQIGESDTTDTLTNGVSSRRNQLLMDISSELGVASVDGAAEATVKALSALVNKVAPNYKAFGPVLSDIVRDRVRGMFGAAGVKLGQITKRVTDTWQLGEGWASHVVATLVLETREGASSRGGDLASLSTDAASNATAANALIDAAVQKVAADKGIAVAMPSAGGAAGGAVVDSAALDAFAAKVTGADGVLASTAKFVLNQLGVVAPVAEETADENAAVAAAVEAELGADWPKQVEPRFDERKAILFDDRWASAREDLARAYYNNDESALNGSFIGLGKTIADEAAWYAGKSDNADLKAAFNRVAGEAVENASESAENSRFAGDIAVVTGVAPNSIAAQVVNGLLAGGATVVATSHSFKPSVKAWAKQTYREHAAGDAKLWLVPANLSSYRDVDALVAWVGNVQKKTSGATTTILKPAYEPSLFFPFAAPPVHGNLADSGELFESQARLMLWGVERAIAGLAKIGADTDVQHKLHVVLPGSPNRGIFGGDGAYGEVKSAFDAIVNRARAEKVWSSRVTFAHPKIGWVRGTGLMGGNDPLVEVVERHGLKTYSTAEIAVELLNLSTRESRIEAAKAPLDVDLTGGLGNEPIDIKALRAEAMEDAVQAEAANAKNAIAGDESDAAAKTLIKALPSPRAPRQAKVDLADWQNVTARPEDEIVIVSVGELGPWGSGRTRFEAELGIHSDGQVDLSAGAVLELAWNMGLLTWNDSPKPGWYDVDGNLVPEEDIAERYHDEVVARSGVRPFDEGMGNDYKDGTDEEEAEVFLDHDVTFSVPTREIAEEYVKLDETHTSFASDVESGEWNVTRHAGSMIRVPRRAAMTRTVGGQFPKGFDPVKWGIPASMVGDVDKIALWNIVTTVDAYLSAGFTPTEILEAVHPSMVASTQGTGFGGMASMRKLYLDRFLNHEIPTDILQEALPNVVAAHVMQSYIGGYGNMVQPVSACATAAVSLEEGADKIALGKADFVVTGAIDDIGVESVIGFGNMNATANSEEMYAKGIDARFFSRANDRRRGGFVESQGGGTILLTRGDIALKLGLPVAGVIGFVHSYADGAHTSIPAPGLGALAAGMGGKDSKLVRDLARLGVTPDDIAVVSKHDTSTNANDPNESELHNTLAHAIGRADGNPLFVISQKTLTGHAKGGACIFQVNGLTQLFKSGVIPANAALDCVDPKLMRDDHMVWLREPLKVGSVKAGLATSLGFGHVSGFAAIVNPGAFEAAVANTAGVEALNAWHDRANERLAAGQRRLEEGMMGRAALYEPIDNRRFHEDGRGYNAHEVEKAMLLDPNARLSASGYFEV
>NZ_AP012327.1|WP_033500761.1|342758_344441_+|acyl-CoA-carboxylase-subunit-beta
MTDIIASPAVKEAVKIVMEQQERLGKNAAEAVSAAATASATDVPGASQPIRASVLRAAQLAHEAEDHARNRQHVKGKLTARERLDLLLDTGSFEEIGRFCGGDINGGRAGAAVITGFGEVFGRKVAVYAQDFSVRGGTLGVAEGRKICRLMDKALDLKVPIIALIDSGGARIQEGVAALTQYGHLFRKTCDASGFVPQLSLILGPCAGGAVYCPALTDLIIMTRENSNMFVTGPDVVKAATGEVISMDDLGGGYVHNATSGVAHYLGEDEADAIDYARTVLAYLPSNSDLQPPTYAYAATRSDRDVAKRLATIVPDNDRQPYDVLDVIRCIVDYGEFVQVHELFASSAVVGFACVNGHPVGIVANQPNVNAGILDVDSSEKVARFVRLCDAFNLPVVTLVDTPGYKPGAEQEHAGIIRRGAKVIYAYANAQVPLVTVILRKAFGGAYIVMGSKAIGADLNYAWPSSQIAVLGASGAVNIIHRKDLQKAKDAGKDVEALRAKYVADYERTTVNANLSLEMGEIDAMIDPEQTRETIIESLKLLSSKKRVRKTTKHHGNQPL
>NZ_AP012327.1|WP_033500816.1|340863_342759_+|ATP-grasp-domain-containing-protein
MPKNVNKLLVANRGEIALRVVRTAREMGIPTVAVYAEQDRHAQYVQMADDAYLLSGDTYKDTYLNEDLLIDILQRSGADAVHPGYGFLSEVASFAQKVIDAGAAWVGPNPEALVDLGDKITARRVATFAKVPPVPGISQSISDMRLLLDFAHTHGYPLMLKRTDGGGGRGITLVHNDDELRGFYMNHDALQGGDLNEYFVERFIDKGRHVETQCGRDSHGNFTVYSTRDCSVQRRNQKLVEEAPAPFLPSDVTDQLETYSRRLFEAVDYEGLGTCEFMVTEQGKVYFLEVNPRLQVEHTVSEEVCGLDLVREQLTIANGGELTVNHPLRGHSFELRLTCEDPAKNLAPSSGTLTSLRWPSGPGIRVDSGVVEGDTISPKFDSMMGKLVVTASDRAAAVARVRRALSELKVEGVPTPASLFEQIFNDDEFTAEHGAAYDVSTKWLERKYLNKEAASSQGGQPASVSGVTGSLDVDKKESSETFVIEVNNRRVSLTVPNDIVANLTGGAKARDAKRAIQPLRGQGLHHAQKKQQSNDGQSGVIASPMQAVVTRINVAEGQQVAKGDLLVVLESMKMENYVYAPVKGAVTKIFVGPAAGVEAGETLMTIDVTGASGNGKQADGTAVGDTTEGGK
>NZ_AP012327.1|WP_033500760.1|340040_340622_-|biotin-transporter-BioY
MLTLKTTLKTATSGTLGSVLKPALFAVLMWAASAAGAVPIPGTPVPITLQTFVVMLAALMLPWKQAGAAMLMYLAAGAVGLPVFAGGASTMALVGPSAGFLIGFLPAAVVTSLLKGEARTDSLKHGALTAARYLFACVAGCIVLDYLLGFIVQSAITGVALPIVAVASMGFIAGDAVKAVVASAVVAGLAKLK
>NZ_AP012327.1|WP_081962251.1|339104_340016_+|biotin--[acetyl-CoA-carboxylase]-ligase
MMAILDSAMIMPRTCAAADQVLLFDTVGSTNTYAADLVREGVLFGANGADGVNAGKSVWSGHEIVAIAANDQTAGRGRLDHTWTSVPGESFIVSLVVSVPVSIMRDLSVSGWLQMIAGCEMREAIVGAVRDCGGGFDGNIGNIALKWPNDIFVDGRKLGGVLAEMVPITGCGDRVAIVIGVGLNLAIAQARLPIDMATSLQLIAHDLPDAAVMRDAIAARWVQGLRSRLADFEEDPHREAMRAREAMIPICWTLGKSCEAHFVDGTTLRGKAIALNDDASLTIEDQEGVLRRVSTADVGVLSK
>NZ_AP012327.1|WP_033500759.1|336942_339042_+|membrane-protein
MPLMSEVKKTGKRGEKVGTFLSWTLAVLACLWVAWCCAIGPLYWFGGGLSDFGWLNVLYLAVAFAICFSIVVALVRFGHGRRVLPRYFVRRYERLCDEIAGTPTTVLGIMLRHLHDAAHSRPGRALHAMIVWLGRKLTRCTDCWWKLMLVFVIGWLWVPTTLLAAFGADIRSQIREFSWALNQWMGLKQPYIGFFSFVPMDIYPTAHYMWPDNPTYLTDQHNVVLTVFYGAVAAVSRYFTDSNDAGIVVLAALQWLFAAFCCSATANRFFNLPWRRLGVGTFDFSRPERHDCWNMRDYTHPEAGVVARPSRLRAGAKTRFVILLFFMVCPLAVFATISLTKSPLFAFAFVWWFGVWYELHMTHVKVLSTINGKPTRLRKRSIMALTISSCVMLISAKYAWYIILFTLILALINDRKRWKTYLVALLLPTILIHGGIVFLTNTGAIIGGDPIESRGIQLQQIARVAKYNPNGIPADAAEKLEPVFNLDQMAEAYSWQDADPVKSSGIQSKKVSYKWRTVTEDDMKNFNEAWLEIVVANPVIALDAFFAECFGYFDVADLPYVSMDYYVDNDYVQSGNVWIHLYNHDWRDAVAGFAKGWGNIPVIGWVTHGNLYVTLMLLVGAAEVVLRRWRSLSWHLPLLLLMGVMITAPANNFERHMLPVAFVFGFVCLQFWRESRNARLAANANMVSEYEASQVRQNE
>NZ_AP012327.1|WP_004220095.1|361090_361651_+|LemA-family-protein
MGVGIIIAVIVVLIVLWAVSAYNGLVTLRNRVKNGWAQIDVQLKQRTDLIPNLVETVKGYAAHESQVFTQVTQARAGVVQAAQSGDVAQRIAAENQLSRALMNLQVVSENYPQLQANTNFMDLQSQLKSLEEKIAYARQFYNDVVQKYNTQTEVVPTNIIAGLFHFEQAPYFQVDEADRQVPQVKF
>NZ_AP012327.1|WP_033500766.1|361762_363976_+|DUF2207-domain-containing-protein
MAAKQHASRAVRSFGIAIVVTVGIVAALLLFMASDDGGADLSYKTLDYDVQVQSNGDLKVTQHIDMKLADRSDDDGDHPWKQLYQQYTLKESDLTNITDISVRNATTGEAYQQGDIAIPSSYSTDEWNREHAKQWYIADVSQGDSNPQPFDPAKDGLVSGNTDDRSKKIEIGWNIPSITSASSLKFDVTMTMQGVTTAYQDMATFQWEPLGVSNQIPIGKVTGTVTFPEGIDADNSWAWLHTECTSETERDKNGSLKFTVYDVRAGDYVDVVAMFDVGVTGGVQRTRDTTIKNGIMKSEAKQEQQWRDQQRKQARIRLITWIAIAVVGLVLCVIAIIFALRSFNRSQYHGGVEYWRDEPEMSPASAAELLHMVDNKHSNTLTSRKMSASVLSLASRGAIAIYPGPAAMYRGIDMSQANNADIARMIANDPARTRDVGKTSTVVILPVVFDNVQSLQLRPSEQAALDLLVTASERIGSPVFDLDQMNENFSDWENGYKLQEKFTNTCDNEFAMLGATSICGGGAFAAGICAVTLAFLSMLYFGAIGNLALFAVISAPMMFASVFALSYLRLKGLTDNGQYLAGQVLGLKHYMEDFSEFKDRGVTDMTLWGRYMVYATAFGISEKAMKQLLKAYPQLADPHWLDANAADSLLYWSYRSWYFDHRYAGPASFDTNSMDFSQFSANFGDIGAQLESGFADIQSTISAASPSGSFGGSGGSFSGGGFGGSSGGSGSGSFGGR
>NZ_AP012327.1|WP_033500767.1|364074_365481_+|AAA-family-ATPase
MTFIGRQDELAFLEDCYASNKAQLVVLYGRRRVGKTELLAQFARNKQHVFFASPSATKDEQLAAFSRQMFEAGAPAGRYLQQYSDWESALADIVNLPTQDGKRRLIIIDEFPYLAKSDSSLPSVLQNLWDHTLKDSNVMMVLCGSSMSFIEKEMLSEKSPLYGRATGMLKLLPMPYWDAAQFFPDYSAQDNALVYAILGGIPHYLAQFDPSESVENNIRRTILRRGMPLYSETEFLMHQEFREPATYNTILQAVALGATQLNDIAQRTMLSGSAVSTYLKNLMEVHIVEREFPVDAKPVEQSKAMRGLYQVSDSFFRFWYAFVGSNRSELEIGDAKGVYEYEIAPYLHDFAAAPFERMCADWLRRKSMQGELGFRASHVGRWWDSTREIDVVAIDKTNAHVLVGECKFRNKPMDNAMLESLRNKSLALKGGDRAYYLFSLNGFDGGVQAVADRNPSVKLIGIDDLYDL
>NZ_AP012327.1|WP_033500768.1|365574_366438_+|aldo/keto-reductase
MALFRNLGPFHTTAIGHGEMPLTIENNRGHDVGIETLHASLDAGCRHIDTAWAYYTPGEEEQTGEKLVREALDSWKGPREEVTVATKVGLRRAWEGDKPVWPRDGKPEHLIEYGKQSAMALGVDSIDLLYLHRHDPEVPYNESCEGIKALLDQGVAQWAGVSNVSIEQLKIAQEILGDKLVAVQNQYSPIHLDTQDTLDYCAEQGLAFVCWSPLGGYRHPYDEHLFDPFREVAEARGVSYQRVVLAWELAKGDHMFVIPGAHRPETILDSLKADELELTEAELAKLG
>NZ_AP012327.1|WP_033500769.1|366600_368139_+|U32-family-peptidase
MQRRNKPEVLAPAGNLRGLKTAVDYGADAVYCGGKAFGMRSAPKNLSLEDFEEGSRYAHERGARVYVTCNVLPRNNEVEAMREYIGQLKDTGVDALIVSDIGVMLMAREVAPNLELHVSTQAGVTNYQAANAFYQLGARRVVLAREMDLQAVRDIRARIPDDLDIECFVHGAMCMAFSGRCLFSNYLTGRDGNHGECAQPCRWKYSIVEEKRPGQYFPIEQTKEGAYLFNSQDMNMLAHIDDLLDSGATSLKIEGRSKSAYYIAAMTNAYKTAVDAYMVQRGFEDEDGTVLKPFHDRVIRPSDPDYGKPETTDSIMEHADGAFAGKPDIAAVPVASAGEPDDLSYHARSTRRKSNTAAEILPEGWYHAGVRPAEHVSLPDWLLDEPDKVAHRDYSTGFYYPEHKVQQSTDRSAYFRAWLVVGEVLSWSPEDGGRVTIMSRNKIEAGQEVEFVLPGAAPLAYTVPAGGFRDADGHWVEAINNPAHVFSMPCPQEVPVNAAIRSRTKKPTLKAE
>NZ_AP012327.1|WP_033500770.1|368222_369239_+|laccase-domain-containing-protein
MTDRQYDDAILDSNAIAPTDVDGNPIPVTIPIMLAPGVKVVYTTRLGGTSEGDYAHCNLGGKTGDDPNHVLANRVALSNAIDAKLSLVSQVHSGVAVDVDESFVMNRPFGFDVSGSKGETAQQVGESADEPAVVEADGQVTAQKNVALGMFAADCLPVLMADAEAGVIGAAHCGRRGLQNGVIGATVEKMVAKGAKPERIVATLGPCICGDCYEVGGDIADEFDAQFPGTFTLSRFGQPGIDIAAAALQELAKTGVPADNVVSSRARVNAATQYLSEDEELAEICQTDGEGEPQLAERFKKIRRSLCTLENPLWFSHRRATIAGKAHEGRMLALIVRE
>NZ_AP012327.1|WP_033500771.1|369326_370154_+|2-C-methyl-D-erythritol-4-phosphate-cytidylyltransferase
MSEREQMQEQKMPVVAVVLAAGFGTRFDENNPKQLVSVGDKPIVCWSIEAFECNDRVSDIIVVVNERVSNMVNMLIDEAGYTKVRAVIPGGSERVDSTRAALDLLKQAGIPDGAKILIHDSVRPFVEQQSIDGCIDSLDQFNAATVAYASTDTILLTEDLGERKVVRSVPDRTNAFRAQTPQAFRFGTITKAYELATADPDFHPTDDTRVVVDYLPNEPVAIVAGSETNLKITTQADMPIAENIARSLDPEHAKQEAKASMHAVFAEAFSQMHKH
>NZ_AP012327.1|WP_033500772.1|370196_370886_+|pyroglutamyl-peptidase-I
MQQINIVISGFTPYDGIDVNPAVLVPEALAEYWNDPSQSVALSDDLLQDVSISVTTVTLPVSFANAWPMLLDAIEQANPDIVIATGLKRSARGVLLERCATNLMDAAKPDADNVIPPRRPINPEGPAAYWTRLPLRSILRDFAKHEIPAALSSDAGTFVCNSLFYHLLDWSAAQEKRILSGFVSLPIVNEQPHPQHGLSIAQQIAAGNDVIRESVRYYLQPSSSEILLG
>NZ_AP012327.1|WP_048349492.1|371034_372513_+|cell-division-protein
MVEQFPIVLRGYDKERVDEAFASAQETVDRLREQVKADDQTILELQAQLQEEKNKKANPNSFASLGANAQQMLASAEQTSAELLDRAKKDASAARATVQLQAETLLNNAKLDAKRILDEAKAKAEAVLSKANNDAGTLKSNAQREADQLRGEAQKAVVAQRQTVDLELTNSREEHDKRLASERATQERELSDMRAEATEQIAAQRKNANDEINRMKSETNDQIESALAEANKKLADVREQVSKMMTDAQRRASEITDTAKAKAQEITDEAEVHRTKTISQVNAEVEQIRADISAQQDEATKKVNELLADLNERRETAKKQSEELISEAQHTRDEAEAYASDKREAADKQAASILKQAAEEADEQINERREAAKSELEGVQQRIADLQTREAQITQRVSELRAMFANAFSGFGANGLSVVNSIDGEQYESAAEAETPAESHDESGETVAGEPEVTFDEDVVSDEQQNNDDFEEPSEDEQSDQGEEAQEEQNND
>NZ_AP012327.1|WP_033500774.1|372505_373846_+|C1-family-peptidase
MTDITPLSADALATLVEDFEASDHNRMAMNAVTAAGINKVARNYDRARLMQRRFSTIVDNGTATHQDRSGRCWLFSSLNVARFVAKKNMGLKEFEFSQNYAMYYDKLERVNYFLQDVAELVKAGEPSDSRLIQHLLADVMGDGGQWTMAMNVYKKYGAVPKDLFPETESSKNTGEMNVQLRRLLHTAVAHMYADPASIESVIAEATAAGHRILTIHLGEPPKSFDWEWTDKDGEFHRDGEITPVEFWQKYVGSADLESYVCLVDDPRQEHAKGKKIGIEHLGNVAGGDPTEYLNVPNQFMKDCVRQILEEQGIPVWFGADCHPMMDRENGAWATDLFEYGKVYGVDFDLNKEDRVRFADSAMNHAMAFVGVDVAEDGTTTRRWRVENSWGDKIADKGYFTMSDDWFTEYVYEVAVPKALLPAEYQAALDEPATMLPAWDPMGALAD

Crispr_ID: NZ_AP012327_3

• CRISPR_ID: NZ_AP012327_3
• CRISPR_location: 2098427-2098569
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 2 spacers

Consensus repeat of NZ_AP012327_3

Consensus_repeat	Method
GCCTCCCACAGTCCAAATTCCTCCACCCACTGGAGGA	PILER-CR

spacers of NZ_AP012327_3

>3.1|2098464|26|NZ_AP012327|PILER-CR
ACAATCAGTACACCCACAGGCCTTCT
>3.2|2098527|16|NZ_AP012327|PILER-CR
AGACCCCCACTAACGG

CRISPR arrays and Neighbor proteins around NZ_AP012327_3

CRISPR arrays

The CRISPR arrays of NZ_AP012327_3

>merge|NZ_AP012327|3|2098427-2098569|PILER-CR
GCTTCTCACAGACCAAATTCCTCCAGCCACTGGAACAATCAGTACACCCACAGGCCTTCTACGGTCCGAAAACCTCCACTCACTGGAGGAAGACCCCCACTAACGGGCCTCCCAGCATCCAAATTCCTCCACCCACTGGAGGA

>NZ_AP012327|3|1|2098427-2098569|PILER-CR
GCTTCTCACAGACCAAATTCCTCCAGCCACTGGAACA	ATCAGTACACCCACAGGCCTTCTACG
GTCCGAAAACCTCCACTCACTGGAGGAAGACCCCCAC	TAACGGGCCTCCCAGC
ATCCAAATTCCTCCACCCACTGGAGGA

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
NZ_AP012327.1|WP_033501950.1|2114013_2114889_-|carbohydrate-ABC-transporter-permease	unknown	unknown	gnl|CDD|223472
NZ_AP012327.1|WP_033501491.1|2090745_2091924_-|DNA-(cytosine-5-)-methyltransferase	unknown	unknown	gnl|CDD|365904
NZ_AP012327.1|WP_033501956.1|2105663_2107034_-|ATP-binding-protein	unknown	unknown	gnl|CDD|379306
NZ_AP012327.1|WP_033501949.1|2115877_2117170_-|carbohydrate-ABC-transporter-substrate-binding-protein	unknown	unknown	gnl|CDD|224567
NZ_AP012327.1|WP_033501962.1|2114927_2115863_-|sugar-ABC-transporter-permease	unknown	unknown	gnl|CDD|224096
NZ_AP012327.1|WP_154304303.1|2111133_2111301_-|hypothetical-protein	unknown	unknown	unknown
NZ_AP012327.1|WP_033501513.1|2088302_2088617_-|restriction-endonuclease	unknown	unknown	gnl|CDD|225973
NZ_AP012327.1|WP_157872378.1|2107304_2107457_+|hypothetical-protein	unknown	unknown	unknown
NZ_AP012327.1|WP_081962280.1|2093824_2094505_-|AAA-family-ATPase	unknown	unknown	gnl|CDD|316042
NZ_AP012327.1|WP_033501492.1|2092021_2092783_+|helix-turn-helix-transcriptional-regulator	unknown	unknown	gnl|CDD|238045
NZ_AP012327.1|WP_033501493.1|2092880_2093174_-|hypothetical-protein	unknown	unknown	unknown
NZ_AP012327.1|WP_033501495.1|2095669_2095864_-|helix-turn-helix-domain-containing-protein	unknown	unknown	gnl|CDD|378935
NZ_AP012327.1|WP_033501957.1|2104762_2105518_+|NAD-dependent-deacetylase	unknown	unknown	gnl|CDD|238698
NZ_AP012327.1|WP_081962281.1|2094590_2095667_-|hypothetical-protein	unknown	unknown	gnl|CDD|173779
NZ_AP012327.1|WP_033501951.1|2112191_2113607_+|ATP-binding-protein	unknown	unknown	gnl|CDD|224586
NZ_AP012327.1|WP_033501954.1|2108931_2110752_-|alpha-glucosidase	unknown	unknown	gnl|CDD|200472
NZ_AP012327.1|WP_033501955.1|2107440_2108706_-|threonine-ammonia-lyase	unknown	unknown	gnl|CDD|236182
NZ_AP012327.1|WP_033501490.1|2089815_2090733_-|DUF4928-family-protein	unknown	unknown	gnl|CDD|374468
NZ_AP012327.1|WP_033501494.1|2093410_2093812_-|hypothetical-protein	unknown	unknown	unknown
NZ_AP012327.1|WP_157872376.1|2089537_2089696_+|hypothetical-protein	unknown	unknown	unknown

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
NZ_AP012327.1|WP_033501491.1|2090745_2091924_-|DNA-(cytosine-5-)-methyltransferase	gnl|CDD|365904	pfam00145, DNA_methylase, C-5 cytosine-specific DNA methylase.	2.23471e-46
NZ_AP012327.1|WP_033501956.1|2105663_2107034_-|ATP-binding-protein	gnl|CDD|379306	pfam13635, DUF4143, Domain of unknown function (DUF4143). This domain is almost always found C-terminal to an ATPase core family.	7.16784e-36
NZ_AP012327.1|WP_033501949.1|2115877_2117170_-|carbohydrate-ABC-transporter-substrate-binding-protein	gnl|CDD|224567	COG1653, UgpB, ABC-type sugar transport system, periplasmic component [Carbohydrate transport and metabolism].	6.46902e-35
NZ_AP012327.1|WP_033501962.1|2114927_2115863_-|sugar-ABC-transporter-permease	gnl|CDD|224096	COG1175, UgpA, ABC-type sugar transport systems, permease components [Carbohydrate transport and metabolism].	1.43883e-69
NZ_AP012327.1|WP_081962280.1|2093824_2094505_-|AAA-family-ATPase	gnl|CDD|316042	pfam13481, AAA_25, AAA domain. This AAA domain is found in a wide variety of presumed DNA repair proteins.	3.52388e-23
NZ_AP012327.1|WP_033501513.1|2088302_2088617_-|restriction-endonuclease	gnl|CDD|225973	COG3440, COG3440, Predicted restriction endonuclease [Defense mechanisms].	4.51952e-08
NZ_AP012327.1|WP_033501492.1|2092021_2092783_+|helix-turn-helix-transcriptional-regulator	gnl|CDD|238045	cd00093, HTH_XRE, Helix-turn-helix XRE-family like proteins. Prokaryotic DNA binding proteins belonging to the xenobiotic response element family of transcriptional regulators.	0.00342137
NZ_AP012327.1|WP_033501495.1|2095669_2095864_-|helix-turn-helix-domain-containing-protein	gnl|CDD|378935	pfam12728, HTH_17, Helix-turn-helix domain. This domain is a DNA-binding helix-turn-helix domain.	1.65035e-06
NZ_AP012327.1|WP_033501957.1|2104762_2105518_+|NAD-dependent-deacetylase	gnl|CDD|238698	cd01407, SIR2-fam, SIR2 family of proteins includes silent information regulator 2 (Sir2) enzymes which catalyze NAD+-dependent protein/histone deacetylation, where the acetyl group from the lysine epsilon-amino group is transferred to the ADP-ribose moiety of NAD+, producing nicotinamide and the novel metabolite O-acetyl-ADP-ribose. Sir2 proteins, also known as sirtuins, are found in all eukaryotes and many archaea and prokaryotes and have been shown to regulate gene silencing, DNA repair, metabolic enzymes, and life span. The most-studied function, gene silencing, involves the inactivation of chromosome domains containing key regulatory genes by packaging them into a specialized chromatin structure that is inaccessible to DNA-binding proteins. The oligomerization state of Sir2 appears to be organism-dependent, sometimes occurring as a monomer and sometimes as a multimer.	1.1069e-93
NZ_AP012327.1|WP_081962281.1|2094590_2095667_-|hypothetical-protein	gnl|CDD|173779	cd01029, TOPRIM_primases, TOPRIM_primases: The topoisomerase-primase (TORPIM) nucleotidyl transferase/hydrolase domain found in the active site regions of bacterial DnaG-type primases and their homologs. Primases synthesize RNA primers for the initiation of DNA replication. DnaG type primases are often closely associated with DNA helicases in primosome assemblies. The TOPRIM domain has two conserved motifs, one of which centers at a conserved glutamate and the other one at two conserved aspartates (DxD). This glutamate and two aspartates, cluster together to form a highly acid surface patch. The conserved glutamate may act as a general base in nucleotide polymerization by primases. The DXD motif may co-ordinate Mg2+, a cofactor required for full catalytic function. The prototypical bacterial primase. Escherichia coli DnaG is a single subunit enzyme.	0.000101529
NZ_AP012327.1|WP_033501951.1|2112191_2113607_+|ATP-binding-protein	gnl|CDD|224586	COG1672, COG1672, Predicted ATPase (AAA+ superfamily) [General function prediction only].	6.9731e-33
NZ_AP012327.1|WP_033501954.1|2108931_2110752_-|alpha-glucosidase	gnl|CDD|200472	cd11333, AmyAc_SI_OligoGlu_DGase, Alpha amylase catalytic domain found in Sucrose isomerases, oligo-1,6-glucosidase (also called isomaltase; sucrase-isomaltase; alpha-limit dextrinase), dextran glucosidase (also called glucan 1,6-alpha-glucosidase), and related proteins. The sucrose isomerases (SIs) Isomaltulose synthase (EC 5.4.99.11) and Trehalose synthase (EC 5.4.99.16) catalyze the isomerization of sucrose and maltose to produce isomaltulose and trehalulose, respectively. Oligo-1,6-glucosidase (EC 3.2.1.10) hydrolyzes the alpha-1,6-glucosidic linkage of isomaltooligosaccharides, pannose, and dextran. Unlike alpha-1,4-glucosidases (EC 3.2.1.20), it fails to hydrolyze the alpha-1,4-glucosidic bonds of maltosaccharides. Dextran glucosidase (DGase, EC 3.2.1.70) hydrolyzes alpha-1,6-glucosidic linkages at the non-reducing end of panose, isomaltooligosaccharides and dextran to produce alpha-glucose.The common reaction chemistry of the alpha-amylase family enzymes is based on a two-step acid catalytic mechanism that requires two critical carboxylates: one acting as a general acid/base (Glu) and the other as a nucleophile (Asp). Both hydrolysis and transglycosylation proceed via the nucleophilic substitution reaction between the anomeric carbon, C1 and a nucleophile. Both enzymes contain the three catalytic residues (Asp, Glu and Asp) common to the alpha-amylase family as well as two histidine residues which are predicted to be critical to binding the glucose residue adjacent to the scissile bond in the substrates. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase.	0
NZ_AP012327.1|WP_033501955.1|2107440_2108706_-|threonine-ammonia-lyase	gnl|CDD|236182	PRK08198, PRK08198, threonine dehydratase; Provisional.	0
NZ_AP012327.1|WP_033501490.1|2089815_2090733_-|DUF4928-family-protein	gnl|CDD|374468	pfam16280, DUF4928, Domain of unknown function (DUF4928). This family consists of uncharacterized proteins around 330 residues in length and is mainly found in various Bacteria species, such as Enterobacteriales, Clostridiales, Actinomycetales and so on. The function of this family is unknown.	1.53957e-52
NZ_AP012327.1|WP_033501950.1|2114013_2114889_-|carbohydrate-ABC-transporter-permease	gnl|CDD|223472	COG0395, UgpE, ABC-type sugar transport system, permease component [Carbohydrate transport and metabolism].	3.20042e-67

Protein sequences

>NZ_AP012327.1|WP_033501495.1|2095669_2095864_-|helix-turn-helix-domain-containing-protein
MAAIPTKNDYPRLTAKPAQVAEMLGYEDVKSVYRLIRTGKIRARKVGNTFLVNLTSVREFAGEE
>NZ_AP012327.1|WP_081962281.1|2094590_2095667_-|hypothetical-protein
MATSYERVLEAARRYPRDLRLQELPRDTSGRQHAQCSCSAHDGDSGTSLHITYDQSNGKTMLHCFGAEHCDTASIAGALGLKIIDLFDQQSGPQGHADYSGYNSYSCDSRLTAADLAAIRHANVTNAPLLSRNETTTITPAHTENSAEIRQRYEMPEPYLEPLALGRSPMFAAVCAAIAVQPNTGLLHGTCPNCGAADSLTVLYSPLESATLLRCSTCQADDVLNALNAPATLARSNGYEAMIYDSERGTAYEYADGLIVRRSPTKRISQSGNTADKHPLWMAADAKRAMASGEPVYLIEGEKDAGTLRALGYAAVTAAGGGGNFAAKLDLDSARLALSGADVVAIVDKDDTGTRWKE
>NZ_AP012327.1|WP_081962280.1|2093824_2094505_-|AAA-family-ATPase
MSGLEFEILPSESSQPESELKTELMPRTYLLTRLADVKPERPLWLRPGHIPKGKIVLIDGDPATGKSTLALDIAAHVTTGTSMAGWVCWMRPRKRAAADCGRWTGGHREPTHSRRTRRRIQDVRPIGSFQGWQGGSDYAHRRTATEIAQAVREQHIGLIVVDVLISFLGSDTNSYKGQDIRAQVMAPLKRLCEETGCTVIALRHLNKGQGAAIYRRGGSIGIVGAA
>NZ_AP012327.1|WP_033501494.1|2093410_2093812_-|hypothetical-protein
MAKDPENGGGQRLFAPVKFNLGSMPRTLAYRLEDNPLLGCAHVRWLGETDDTVESLNQSAYGTSEREDSDVRTFIQDYFDHNKELTPDGLYGGVPSYRVINEAKGEFSKQQVLDGRRRPKPLLASKKYGNMWW
>NZ_AP012327.1|WP_033501493.1|2092880_2093174_-|hypothetical-protein
MGDPEKTQDLQCLTANEPTSELVRRQDGASENATHHPATGEADGSVTDIAGWKASLPPYATDLHYQQLNATQLNELIGKGGLWASKARREIARRECE
>NZ_AP012327.1|WP_033501492.1|2092021_2092783_+|helix-turn-helix-transcriptional-regulator
MTKLSDAVNASNLDFHELARRSNVSEKRLNGYLSGRHSFSRAPLEDAVAIANALGGRIEDYMEDAAEDGEAEKLDDRLRLQLFLDSVDEYELIMKNVGRPQAVTIGGSGEKTEKRYSQLIVEGMLLRKYYSSDEVQLAKVIDSFRRLIPDDASPDVLHTVDPLEKDTPSQGNKSQIVFSNADGSYSSGVDLLFDVLYGITLHGDPDRIQRLARWPITAQCLALYESNVERRKGVVTVRQYIDLAIKDGLFALD
>NZ_AP012327.1|WP_033501491.1|2090745_2091924_-|DNA-(cytosine-5-)-methyltransferase
MNDNHTHAATGKACPQALEFFAGIGLARLGLEESGFQVEWSNDIDSAKCEMYRNNFTDTPGHTLVEGDMSELSGDDLPHDASIAWGSSPCTDLSLAGTRTGLNGKQSGAFWQYVRLLEELGDSRPPIAILENVNGLATSHSGEDLAAAVHAFNVLGYSVDVLSIDTRRFIPQSRPRLFLVGLQNPPSAEQFDSSLRPDWLQQVFADTSLRTHRAPLPEPPSPLMSGLNMYVENMADDDERWWDAERTAKFLDSLSPVQTARVETLRNETTVSYRTAYRRTRNGKAVWEVRADDISGCLRTARGGSSRQAIIRMGNGQAAVRWMTPREYAALMGAAHYNITGLRSNQVMFGFGDAVSVPVVEWLGKHYFMPALNNTLGKDLYVEQTLLSNHTA
>NZ_AP012327.1|WP_033501490.1|2089815_2090733_-|DUF4928-family-protein
MDLTPIDDFRKSHQVNTKGALAAAIQLNRFFSADTLPIDASDYHTNKEGQVKGISKDNCQKILAEYGITRLLAAEGGRTSRGTMALMHDYAELINELRPTAEQFTEIEQYWVKRIQAFFTSKPFKLESDNSLSVDAAVEHLLQQAAQRQKENPGTMYVGTVLQHLVAAKLTIVAPEVEINGASVADDPTGRGGDFNVGDTAIHCITAPASLLMNKCQRNIKAGLHPIIITVRDRVKTAWDLASDMGFENRLEVWDLQSFLSSNVHEHGHFTNAARHETLSRLVKAYNQIIDEHESDPSLHIEYNG
>NZ_AP012327.1|WP_157872376.1|2089537_2089696_+|hypothetical-protein
MEIQWQAKMYVGISMISTFPRTPVGTILRQHGTYMEIYSAYMEISLHQSVGA
>NZ_AP012327.1|WP_033501513.1|2088302_2088617_-|restriction-endonuclease
MQLPTLLIASHIKPWKASTPTEKTAASNGLLLNAFHDRAFDQGLISIDNDYCIMVNHDKVKHSDINDKWLYDFEGREISLPAISQPSHEFIEYHHKHVFLADAA
>NZ_AP012327.1|WP_033501957.1|2104762_2105518_+|NAD-dependent-deacetylase
MSKKIAVLTGAGISTSAGIPDFRGPDGVWTKHPEQTKVYDIDAFLANEEDRVYSWRWQKESPVWNAQPGTAHKALVKLEQAGLLTLLATQNFDALHEKAGNSSDVIVNLHGTIGTSHCMKCHAKYDTADIMANLDNEPDPHCHRRLPYSGNMPCNGLIKTDVVYFGEALPDGALEKSYRLATQADELWVIGSTLEVMPAASIVPMAAQSGVPITIMNMGRTQYDRLADRLIHDDIAVALPQLVDETIAAAQ
>NZ_AP012327.1|WP_033501956.1|2105663_2107034_-|ATP-binding-protein
MTKNSNQQVKPQGYRPRIVDQQIERYMRIFGAVEIAGTKWCGKTWSARQHAASITYVDRGNNLMAAQSDPSLMLLGERPHVIDEWQLVPPIWDEVRHKVDDEPGEKGQWLLTGSSTPMHDKRPKHTGAGRIGHVRMSPMSLFESGDSTGEVSLSSLFEGDFNPAKVETPTERLFDFVCRGGWPEAQNLPVSDAQILIREYVHLTLNEGVSRQRRDPEVARRLLRSLSRNVSQSVTFKTLGLDMYGAELDLDSDVADKQNDSQKKDLQKDLEDFISPRTISEYVAMFERMFVIDGVPGWVPPSRSPKRLQTKPRRYFADPSIAAAVLGVNPRALLEDWQTFGFLFENLCVRDLLVYARALPDIGDEPVRYYHDDSGLECDAIIELTDGRWAGIEIKSSQSKVDDAAKNLLRLREKLLKNPQSRVRAPEFLAVLVGVGDFAYQREDGIYVIPITALGA
>NZ_AP012327.1|WP_157872378.1|2107304_2107457_+|hypothetical-protein
MTEQLAAQQSNKATKRVLEGFATINNHRKAFQDPYLLKLVLKSEEISKYA
>NZ_AP012327.1|WP_033501955.1|2107440_2108706_-|threonine-ammonia-lyase
MEQNEVLKALQRNHAAELKLAAERLEGTARHTEIIPSKVLSEMTGHEILLKPENLQVTGSFKIRGAYNKIASLSDEQLARGIVTASAGNHAQGVAYAARERGAKATICMPQITPPLKVDATKAYGADVVLYGEVFDESAARAQELSDKEGMIYVPPFDDYEVLCGQGTIGLEILEDVPNVTDVVVPLGGGGLGAGVALAIKTFKPEVRVIGAIPEGSPAWKNSLAAGHVVAADQVRTSAEGVAVKRPGDLTFALLNEFLDDIVTVTERDINEMILLMLEKHKLVVEAAGAVSLAALEHLNLRSRKFAEAKGPHVVVPILSGGNEDTVSISAVIQKGMIARGRIMNFEVELPDTPGQLVKVATILAEQRANVIALDHDQFKASSHYTDSVSLGVTVETNGPEHIDRILDAMREAGFQPKRIY
>NZ_AP012327.1|WP_033501954.1|2108931_2110752_-|alpha-glucosidase
MTDFNRSILPDAVRSNGATPNPWWANAVVYQIYPRSFQDTNGDGVGDLKGITSRLDYLADLGVDVVWLSPVYRSPQDDNGYDISDYQDIDPLFGTLEDMDELLAEAHRRGLKIVMDLVVNHTSDEHAWFQASRDKDDPHADWYWWRPARPGHEPGTPGAEPNRWGSYFGGSAWQYDPKRGEYYLHQYSRKQPDLNWENPEVRKAVYRMMNWWMDRGIDGFRMDVITQVSKTVDKNGKLPGEDGSEIEDYPVGEEGYSSPFSWCSDGPRIDEFLAEMRREVFEGREGYMNVGEAPGITPDRNEFITDPKNGELDMLFLFDHVGIDQEGSKWNTVPFEVRNLRTRLAAQQEAVKDAGWASLFFCNHDQPRVVSRWGDDSDRESRELGAKAFGMLLHMHRGTPYIYEGEELGMTNAHFTSLDQYRDLEAINAYRQRVEEARCQSPESMMAALALIGRDNARTPMQWDASRYAGFTAPDAAAEPWIGVNPNHVEINAAEEFDDPDSVYGFYKKLIAMRHNNATVATGEWNLLAADDEQVYAFTRTNGDDTILVVVNLTDKSAGLPGEVAELLGDGVAESQILLSTYDAVHGVKSIARGELARWEGVVIQL
>NZ_AP012327.1|WP_154304303.1|2111133_2111301_-|hypothetical-protein
MAAKSACPWNGVRVDASDVRVQRLINTSLHDLDGLRMSIPALPEDRFLAVDDFMI
>NZ_AP012327.1|WP_033501951.1|2112191_2113607_+|ATP-binding-protein
MFVGRKQELATLEQQFSRHRFEMTVIYGRRRIGKTALIDQFVANKKTLYFTALQRSSAINLHLFTIQIANFFELPTLPDFTDWYSALQFIIAQAKTLNERFVFVFDEFPYAALMSESLPSEMQIAIDHGFKDLNCMIILSGSNEGFMESEVLGYKSPLYGRRTSQIRLQPLNYTAIGEFVPQSSVFDRINYYATFGGTPYYLTQLDPDADFETNVLNLCFNQLGLLHEEPMMLLREELREPALYASVLQAIAGGSSTPKIIAEHSGVDPNSINKYLRILEELGLVSRNVPFGERPEKSRKALYSIKDPFFAYWYQFVAPAQSAIESHAGPNAARQLAFNDAFTTYVGQQFEVICLQWLLKHNGENGKKCAVPFLATQFGKWWGNDPRKREQTDIDIVLGNPQTKQLLMCECKWRNTLKETEAVEALRDRVGLIRGYGETHLMFFSKYPISDATQRKYEGQVTFISAEQLFA
>NZ_AP012327.1|WP_033501950.1|2114013_2114889_-|carbohydrate-ABC-transporter-permease
MTSATVAKTGVQAQAKPEKIRRDHKVNWWLTAVVAVLSLTVLVPLYFAVVTALKTPGEAGTFSLPTTWEWHNFADAWNKVNYPKAALNSAIITVCAVVLTLLTNTFVAYAVARNMDKRFFRFLYYFFLAMMFVPFTVIMLPIAKEMGSLHLDNQLGLIILYTILGIGTNLFIAIGFIRSIPISLEEAARIDGASTWRIFWTIIFPLMGPINATIAILTALWAWNDFLLPLITLTDQSNQTIPLAQYVFQSQFTSNYPMAFASYLMAMAPVLIVYIFAQKWVVGGVMRGAVK
>NZ_AP012327.1|WP_033501962.1|2114927_2115863_-|sugar-ABC-transporter-permease
MTTAHTANTAKKSAKTSKNKVSKYSKRKVDPAFYWMTVPAVILVSIFLYWPFLQGAAYSFTNSQGYGDFKFIGLKNYIAMFQDSRVGHAYLFTIFIAILITVGTNFLGMFLAVLLNSKIAFKNGFRAIFFIPYTLSVLVIGYVFKYIFMTPLPAIGQALHIDWLSTSMISNPDLAWFPIVFLSIWQGVAYSTLLYLAGLQTIDNEIYEAAAIDGVNAWQNFWQITFPLIGPFFTINMVLSLKNSLGAFDQIMALTKGGPDSKTETVSYLIFQNGLGNGEYSYQMANAVTFFIVLAILAFVQLKFFSGKEKV
>NZ_AP012327.1|WP_033501949.1|2115877_2117170_-|carbohydrate-ABC-transporter-substrate-binding-protein
MKPDSTTSVGKFRRIVYRTLTAVCAVALTAGLAGCGNSTAGTVTLDFFQFKAEAADWFTAKAKEFEKTHPNIKINVNNSSDATTDLRTRLVKNREPDVITINGDINFGMLAEAGVFHDFTDDDIVDELNPGMVNIAKSLVQTNDESKKRLYGLPYAGNASGYIINADVWEQAGEDPDNPPQTWSEFIDLLQRFKSKGIVPIEASTADSWTVQAPLASLNSTLVPESEYLSLKDGSKKFSDLWGTVSDQLVEIYQNYTQKNPAVTYQQATQDLAAGKAAILPLGTYAIPQIRLINSDANLRFAQMPATDNAEEQQLTAGDDVILTMGAHTKHEKEAREFIDFLMNDENIKDYSKRQSAFTPYKDTYVGDEALNGVLDFYRAGKLADFCDHYVPASINLAGFLQTLIQSGNTEKFLNSMQSEYDKIEARNFR

Crispr_ID: NZ_AP012327_4

• CRISPR_ID: NZ_AP012327_4
• CRISPR_location: 2118657-2118815
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 1 spacers

Consensus repeat of NZ_AP012327_4

Consensus_repeat	Method
TATTTAGCGCTCTCGACGGGCGCAAGCACAGCCTTGAAAAGGACTGCTTGC	CRISPRCasFinder

spacers of NZ_AP012327_4

>4.1|2118708|57|NZ_AP012327|CRISPRCasFinder
CGAACCGTTAAGCGGGAAGCCCAGTGGGCTTCCCGTAGGTGAGGGGAGAGAGCGCGC

CRISPR arrays and Neighbor proteins around NZ_AP012327_4

CRISPR arrays

The CRISPR arrays of NZ_AP012327_4

>merge|NZ_AP012327|4|2118657-2118815|CRISPRCasFinder
TATTTAGCGCTCTCGACGGGCGCAAGCACAGCCTTGAAAAGGACTGCTTGCCGAACCGTTAAGCGGGAAGCCCAGTGGGCTTCCCGTAGGTGAGGGGAGAGAGCGCGCTATTCAGCGCTCTCGACGGGCGCAAGCACAGCCTTGAAAAGGACTGCTTGC

>NZ_AP012327|4|2|2118657-2118815|CRISPRCasFinder
TATTTAGCGCTCTCGACGGGCGCAAGCACAGCCTTGAAAAGGACTGCTTGC	CGAACCGTTAAGCGGGAAGCCCAGTGGGCTTCCCGTAGGTGAGGGGAGAGAGCGCGC
TATTCAGCGCTCTCGACGGGCGCAAGCACAGCCTTGAAAAGGACTGCTTGC

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
NZ_AP012327.1|WP_004223871.1|2125338_2125920_+|dCTP-deaminase	unknown	unknown	gnl|CDD|234753
NZ_AP012327.1|WP_033501950.1|2114013_2114889_-|carbohydrate-ABC-transporter-permease	unknown	unknown	gnl|CDD|223472
NZ_AP012327.1|WP_033501956.1|2105663_2107034_-|ATP-binding-protein	unknown	unknown	gnl|CDD|379306
NZ_AP012327.1|WP_033501963.1|2134297_2137294_-|cation-translocating-P-type-ATPase	unknown	unknown	gnl|CDD|223550
NZ_AP012327.1|WP_033501959.1|2124371_2125106_+|esterase	unknown	unknown	gnl|CDD|223477
NZ_AP012327.1|WP_128832457.1|2139717_2140935_-|MFS-transporter	unknown	unknown	gnl|CDD|340874
NZ_AP012327.1|WP_157872378.1|2107304_2107457_+|hypothetical-protein	unknown	unknown	unknown
NZ_AP012327.1|WP_048349547.1|2137636_2138890_-|hypothetical-protein	unknown	unknown	gnl|CDD|238881
NZ_AP012327.1|WP_051920323.1|2126474_2127350_+|aminotransferase-class-III-fold-pyridoxal-phosphate-dependent-enzyme	unknown	unknown	gnl|CDD|180426
NZ_AP012327.1|WP_033501960.1|2121205_2121655_-|nucleoside-deaminase	unknown	unknown	gnl|CDD|223663
NZ_AP012327.1|WP_033501954.1|2108931_2110752_-|alpha-glucosidase	unknown	unknown	gnl|CDD|200472
NZ_AP012327.1|WP_033501949.1|2115877_2117170_-|carbohydrate-ABC-transporter-substrate-binding-protein	unknown	unknown	gnl|CDD|224567
NZ_AP012327.1|WP_033501962.1|2114927_2115863_-|sugar-ABC-transporter-permease	unknown	unknown	gnl|CDD|224096
NZ_AP012327.1|WP_128832406.1|2126162_2126285_+|hypothetical-protein	unknown	unknown	gnl|CDD|372570
NZ_AP012327.1|WP_033501951.1|2112191_2113607_+|ATP-binding-protein	unknown	unknown	gnl|CDD|224586
NZ_AP012327.1|WP_033501961.1|2117377_2118580_+|ROK-family-protein	unknown	unknown	gnl|CDD|224851
NZ_AP012327.1|WP_033501964.1|2138911_2139721_-|alpha/beta-hydrolase	unknown	unknown	gnl|CDD|223999
NZ_AP012327.1|WP_033501955.1|2107440_2108706_-|threonine-ammonia-lyase	unknown	unknown	gnl|CDD|236182
NZ_AP012327.1|WP_033501947.1|2121762_2123835_+|sodium:proton-antiporter	unknown	unknown	gnl|CDD|223104
NZ_AP012327.1|WP_154304303.1|2111133_2111301_-|hypothetical-protein	unknown	unknown	unknown

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
NZ_AP012327.1|WP_004223871.1|2125338_2125920_+|dCTP-deaminase	gnl|CDD|234753	PRK00416, dcd, deoxycytidine triphosphate deaminase; Reviewed.	4.37887e-92
NZ_AP012327.1|WP_033501956.1|2105663_2107034_-|ATP-binding-protein	gnl|CDD|379306	pfam13635, DUF4143, Domain of unknown function (DUF4143). This domain is almost always found C-terminal to an ATPase core family.	7.16784e-36
NZ_AP012327.1|WP_033501959.1|2124371_2125106_+|esterase	gnl|CDD|223477	COG0400, COG0400, Predicted esterase [General function prediction only].	8.92284e-20
NZ_AP012327.1|WP_128832457.1|2139717_2140935_-|MFS-transporter	gnl|CDD|340874	cd17316, MFS_SV2_like, Metazoan Synaptic vesicle glycoprotein 2 (SV2) and related small molecule transporters of the Major Facilitator Superfamily. This family is composed of metazoan synaptic vesicle glycoprotein 2 (SV2) and related small molecule transporters including those that transport inorganic phosphate (Pht), aromatic compounds (PcaK and related proteins), proline/betaine (ProP), alpha-ketoglutarate (KgtP), citrate (CitA), shikimate (ShiA), and cis,cis-muconate (MucK), among others. SV2 is a transporter-like protein that serves as the receptor for botulinum neurotoxin A (BoNT/A), one of seven neurotoxins produced by the bacterium Clostridium botulinum. BoNT/A blocks neurotransmitter release by cleaving synaptosome-associated protein of 25 kD (SNAP-25) within presynaptic nerve terminals. Also included in this family is synaptic vesicle 2 (SV2)-related protein (SVOP) and similar proteins. SVOP is a transporter-like nucleotide binding protein that localizes to neurotransmitter-containing vesicles. The SV2-like family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.	2.03651e-22
NZ_AP012327.1|WP_033501963.1|2134297_2137294_-|cation-translocating-P-type-ATPase	gnl|CDD|223550	COG0474, MgtA, Cation transport ATPase [Inorganic ion transport and metabolism].	0
NZ_AP012327.1|WP_048349547.1|2137636_2138890_-|hypothetical-protein	gnl|CDD|238881	cd01844, SGNH_hydrolase_like_6, SGNH_hydrolase subfamily. SGNH hydrolases are a diverse family of lipases and esterases. The tertiary fold of the enzyme is substantially different from that of the alpha/beta hydrolase family and unique among all known hydrolases; its active site closely resembles the Ser-His-Asp(Glu) triad found in other serine hydrolases.	3.51464e-46
NZ_AP012327.1|WP_051920323.1|2126474_2127350_+|aminotransferase-class-III-fold-pyridoxal-phosphate-dependent-enzyme	gnl|CDD|180426	PRK06148, PRK06148, hypothetical protein; Provisional.	7.17973e-53
NZ_AP012327.1|WP_033501960.1|2121205_2121655_-|nucleoside-deaminase	gnl|CDD|223663	COG0590, CumB, Cytosine/adenosine deaminases [Nucleotide transport and metabolism / Translation, ribosomal structure and biogenesis].	9.0803e-58
NZ_AP012327.1|WP_033501954.1|2108931_2110752_-|alpha-glucosidase	gnl|CDD|200472	cd11333, AmyAc_SI_OligoGlu_DGase, Alpha amylase catalytic domain found in Sucrose isomerases, oligo-1,6-glucosidase (also called isomaltase; sucrase-isomaltase; alpha-limit dextrinase), dextran glucosidase (also called glucan 1,6-alpha-glucosidase), and related proteins. The sucrose isomerases (SIs) Isomaltulose synthase (EC 5.4.99.11) and Trehalose synthase (EC 5.4.99.16) catalyze the isomerization of sucrose and maltose to produce isomaltulose and trehalulose, respectively. Oligo-1,6-glucosidase (EC 3.2.1.10) hydrolyzes the alpha-1,6-glucosidic linkage of isomaltooligosaccharides, pannose, and dextran. Unlike alpha-1,4-glucosidases (EC 3.2.1.20), it fails to hydrolyze the alpha-1,4-glucosidic bonds of maltosaccharides. Dextran glucosidase (DGase, EC 3.2.1.70) hydrolyzes alpha-1,6-glucosidic linkages at the non-reducing end of panose, isomaltooligosaccharides and dextran to produce alpha-glucose.The common reaction chemistry of the alpha-amylase family enzymes is based on a two-step acid catalytic mechanism that requires two critical carboxylates: one acting as a general acid/base (Glu) and the other as a nucleophile (Asp). Both hydrolysis and transglycosylation proceed via the nucleophilic substitution reaction between the anomeric carbon, C1 and a nucleophile. Both enzymes contain the three catalytic residues (Asp, Glu and Asp) common to the alpha-amylase family as well as two histidine residues which are predicted to be critical to binding the glucose residue adjacent to the scissile bond in the substrates. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase.	0
NZ_AP012327.1|WP_033501949.1|2115877_2117170_-|carbohydrate-ABC-transporter-substrate-binding-protein	gnl|CDD|224567	COG1653, UgpB, ABC-type sugar transport system, periplasmic component [Carbohydrate transport and metabolism].	6.46902e-35
NZ_AP012327.1|WP_033501962.1|2114927_2115863_-|sugar-ABC-transporter-permease	gnl|CDD|224096	COG1175, UgpA, ABC-type sugar transport systems, permease components [Carbohydrate transport and metabolism].	1.43883e-69
NZ_AP012327.1|WP_128832406.1|2126162_2126285_+|hypothetical-protein	gnl|CDD|372570	pfam13333, rve_2, Integrase core domain.	0.000815927
NZ_AP012327.1|WP_033501951.1|2112191_2113607_+|ATP-binding-protein	gnl|CDD|224586	COG1672, COG1672, Predicted ATPase (AAA+ superfamily) [General function prediction only].	6.9731e-33
NZ_AP012327.1|WP_033501961.1|2117377_2118580_+|ROK-family-protein	gnl|CDD|224851	COG1940, NagC, Transcriptional regulator/sugar kinase [Transcription / Carbohydrate transport and metabolism].	3.76182e-31
NZ_AP012327.1|WP_033501964.1|2138911_2139721_-|alpha/beta-hydrolase	gnl|CDD|223999	COG1073, COG1073, Hydrolases of the alpha/beta superfamily [General function prediction only].	8.74457e-09
NZ_AP012327.1|WP_033501955.1|2107440_2108706_-|threonine-ammonia-lyase	gnl|CDD|236182	PRK08198, PRK08198, threonine dehydratase; Provisional.	0
NZ_AP012327.1|WP_033501947.1|2121762_2123835_+|sodium:proton-antiporter	gnl|CDD|223104	COG0025, NhaP, NhaP-type Na+/H+ and K+/H+ antiporters [Inorganic ion transport and metabolism].	6.97245e-79
NZ_AP012327.1|WP_033501950.1|2114013_2114889_-|carbohydrate-ABC-transporter-permease	gnl|CDD|223472	COG0395, UgpE, ABC-type sugar transport system, permease component [Carbohydrate transport and metabolism].	3.20042e-67

Protein sequences

>NZ_AP012327.1|WP_033501961.1|2117377_2118580_+|ROK-family-protein
MAVSPLPQWFEGSEYTHKVATCVMKYGPISRITLAQILGLSQGAVSRITSDLIYAGVIEETPMPSGKAGKLPKGFVQKENAERRGRPQTGLRIIENARSFVGMKINATHISAVAVNAIGQIITGCHDLEIEDTSPQAVVALIKQLTIDCSDEAEMAGWPKPCAVGISLGGHVVDNAVVTFAPFMHWSQPVELSAMVREATGLPTGIYNDIDSLVVDACMFGSGVGLDSFAVLTFGAGVGYSLTVNGELVSCPDKSYGLVGHILVDPDGPRCVSGHKGCAQCLSDNSIAAEYSQIIGHPTSFDDFARDARANKPQATNLVNRTCFRLGTMVATIANLAMPDKIMIAGESSFIAKFGIDDLRNGINMYRHSQAAPVDFEIPDHDWALWAKAAAAQAIRQYVG
>NZ_AP012327.1|WP_033501949.1|2115877_2117170_-|carbohydrate-ABC-transporter-substrate-binding-protein
MKPDSTTSVGKFRRIVYRTLTAVCAVALTAGLAGCGNSTAGTVTLDFFQFKAEAADWFTAKAKEFEKTHPNIKINVNNSSDATTDLRTRLVKNREPDVITINGDINFGMLAEAGVFHDFTDDDIVDELNPGMVNIAKSLVQTNDESKKRLYGLPYAGNASGYIINADVWEQAGEDPDNPPQTWSEFIDLLQRFKSKGIVPIEASTADSWTVQAPLASLNSTLVPESEYLSLKDGSKKFSDLWGTVSDQLVEIYQNYTQKNPAVTYQQATQDLAAGKAAILPLGTYAIPQIRLINSDANLRFAQMPATDNAEEQQLTAGDDVILTMGAHTKHEKEAREFIDFLMNDENIKDYSKRQSAFTPYKDTYVGDEALNGVLDFYRAGKLADFCDHYVPASINLAGFLQTLIQSGNTEKFLNSMQSEYDKIEARNFR
>NZ_AP012327.1|WP_033501962.1|2114927_2115863_-|sugar-ABC-transporter-permease
MTTAHTANTAKKSAKTSKNKVSKYSKRKVDPAFYWMTVPAVILVSIFLYWPFLQGAAYSFTNSQGYGDFKFIGLKNYIAMFQDSRVGHAYLFTIFIAILITVGTNFLGMFLAVLLNSKIAFKNGFRAIFFIPYTLSVLVIGYVFKYIFMTPLPAIGQALHIDWLSTSMISNPDLAWFPIVFLSIWQGVAYSTLLYLAGLQTIDNEIYEAAAIDGVNAWQNFWQITFPLIGPFFTINMVLSLKNSLGAFDQIMALTKGGPDSKTETVSYLIFQNGLGNGEYSYQMANAVTFFIVLAILAFVQLKFFSGKEKV
>NZ_AP012327.1|WP_033501950.1|2114013_2114889_-|carbohydrate-ABC-transporter-permease
MTSATVAKTGVQAQAKPEKIRRDHKVNWWLTAVVAVLSLTVLVPLYFAVVTALKTPGEAGTFSLPTTWEWHNFADAWNKVNYPKAALNSAIITVCAVVLTLLTNTFVAYAVARNMDKRFFRFLYYFFLAMMFVPFTVIMLPIAKEMGSLHLDNQLGLIILYTILGIGTNLFIAIGFIRSIPISLEEAARIDGASTWRIFWTIIFPLMGPINATIAILTALWAWNDFLLPLITLTDQSNQTIPLAQYVFQSQFTSNYPMAFASYLMAMAPVLIVYIFAQKWVVGGVMRGAVK
>NZ_AP012327.1|WP_033501951.1|2112191_2113607_+|ATP-binding-protein
MFVGRKQELATLEQQFSRHRFEMTVIYGRRRIGKTALIDQFVANKKTLYFTALQRSSAINLHLFTIQIANFFELPTLPDFTDWYSALQFIIAQAKTLNERFVFVFDEFPYAALMSESLPSEMQIAIDHGFKDLNCMIILSGSNEGFMESEVLGYKSPLYGRRTSQIRLQPLNYTAIGEFVPQSSVFDRINYYATFGGTPYYLTQLDPDADFETNVLNLCFNQLGLLHEEPMMLLREELREPALYASVLQAIAGGSSTPKIIAEHSGVDPNSINKYLRILEELGLVSRNVPFGERPEKSRKALYSIKDPFFAYWYQFVAPAQSAIESHAGPNAARQLAFNDAFTTYVGQQFEVICLQWLLKHNGENGKKCAVPFLATQFGKWWGNDPRKREQTDIDIVLGNPQTKQLLMCECKWRNTLKETEAVEALRDRVGLIRGYGETHLMFFSKYPISDATQRKYEGQVTFISAEQLFA
>NZ_AP012327.1|WP_154304303.1|2111133_2111301_-|hypothetical-protein
MAAKSACPWNGVRVDASDVRVQRLINTSLHDLDGLRMSIPALPEDRFLAVDDFMI
>NZ_AP012327.1|WP_033501954.1|2108931_2110752_-|alpha-glucosidase
MTDFNRSILPDAVRSNGATPNPWWANAVVYQIYPRSFQDTNGDGVGDLKGITSRLDYLADLGVDVVWLSPVYRSPQDDNGYDISDYQDIDPLFGTLEDMDELLAEAHRRGLKIVMDLVVNHTSDEHAWFQASRDKDDPHADWYWWRPARPGHEPGTPGAEPNRWGSYFGGSAWQYDPKRGEYYLHQYSRKQPDLNWENPEVRKAVYRMMNWWMDRGIDGFRMDVITQVSKTVDKNGKLPGEDGSEIEDYPVGEEGYSSPFSWCSDGPRIDEFLAEMRREVFEGREGYMNVGEAPGITPDRNEFITDPKNGELDMLFLFDHVGIDQEGSKWNTVPFEVRNLRTRLAAQQEAVKDAGWASLFFCNHDQPRVVSRWGDDSDRESRELGAKAFGMLLHMHRGTPYIYEGEELGMTNAHFTSLDQYRDLEAINAYRQRVEEARCQSPESMMAALALIGRDNARTPMQWDASRYAGFTAPDAAAEPWIGVNPNHVEINAAEEFDDPDSVYGFYKKLIAMRHNNATVATGEWNLLAADDEQVYAFTRTNGDDTILVVVNLTDKSAGLPGEVAELLGDGVAESQILLSTYDAVHGVKSIARGELARWEGVVIQL
>NZ_AP012327.1|WP_033501955.1|2107440_2108706_-|threonine-ammonia-lyase
MEQNEVLKALQRNHAAELKLAAERLEGTARHTEIIPSKVLSEMTGHEILLKPENLQVTGSFKIRGAYNKIASLSDEQLARGIVTASAGNHAQGVAYAARERGAKATICMPQITPPLKVDATKAYGADVVLYGEVFDESAARAQELSDKEGMIYVPPFDDYEVLCGQGTIGLEILEDVPNVTDVVVPLGGGGLGAGVALAIKTFKPEVRVIGAIPEGSPAWKNSLAAGHVVAADQVRTSAEGVAVKRPGDLTFALLNEFLDDIVTVTERDINEMILLMLEKHKLVVEAAGAVSLAALEHLNLRSRKFAEAKGPHVVVPILSGGNEDTVSISAVIQKGMIARGRIMNFEVELPDTPGQLVKVATILAEQRANVIALDHDQFKASSHYTDSVSLGVTVETNGPEHIDRILDAMREAGFQPKRIY
>NZ_AP012327.1|WP_157872378.1|2107304_2107457_+|hypothetical-protein
MTEQLAAQQSNKATKRVLEGFATINNHRKAFQDPYLLKLVLKSEEISKYA
>NZ_AP012327.1|WP_033501956.1|2105663_2107034_-|ATP-binding-protein
MTKNSNQQVKPQGYRPRIVDQQIERYMRIFGAVEIAGTKWCGKTWSARQHAASITYVDRGNNLMAAQSDPSLMLLGERPHVIDEWQLVPPIWDEVRHKVDDEPGEKGQWLLTGSSTPMHDKRPKHTGAGRIGHVRMSPMSLFESGDSTGEVSLSSLFEGDFNPAKVETPTERLFDFVCRGGWPEAQNLPVSDAQILIREYVHLTLNEGVSRQRRDPEVARRLLRSLSRNVSQSVTFKTLGLDMYGAELDLDSDVADKQNDSQKKDLQKDLEDFISPRTISEYVAMFERMFVIDGVPGWVPPSRSPKRLQTKPRRYFADPSIAAAVLGVNPRALLEDWQTFGFLFENLCVRDLLVYARALPDIGDEPVRYYHDDSGLECDAIIELTDGRWAGIEIKSSQSKVDDAAKNLLRLREKLLKNPQSRVRAPEFLAVLVGVGDFAYQREDGIYVIPITALGA
>NZ_AP012327.1|WP_033501960.1|2121205_2121655_-|nucleoside-deaminase
MQWSDDMGLAIELAREAAAEGEVPVGAVVLDAGGAVIGRGRNLREAHADPLAHAEVKAMTQAARSLGTWNLADCTLIVTLEPCPMCAGACLQTHVGRIVFGAWDAKLGACGSIWDIPRDPHIGHVPEVIGGVREAECARLMTDFFAGKR
>NZ_AP012327.1|WP_033501947.1|2121762_2123835_+|sodium:proton-antiporter
MAVFELIVCIIAAVVLSSFISRFIPKVSTPLVQIALGTLASQLPFFPNVTLDPELFMVLFIAPLLYLEAHEIDKSELLKSVKLSLSLAIGLAIATMVAVGFTLHAAWPAIPLAAALALGAALGPTDAVAVSSLGKEAALTQRQTSVLKGESLFNDASGIVGFQFAIAAAISGVFEVGDSAAQFVFSFFGGAIFGLAVGMVADLLFESLRSFGWETTTSRILMELFLPFILYLGAEAVHVSGILSVVAAGLIIRFDHTGIGPNVARTNIVSSSVWGVFSFTLNGTVFILLGMLLPNAMSASWDDPQVSNWRLLVAILTVSAVVIVMRFLWISLMLRLARDTVTGKRRKMTAQRWRSAAVMTFGGPKGTITLSLMFTIPYTITGGAWFPMRDELIFIAGGVIVVTLLLANFLLPLLAPNRNKDASVEMTEITIEVLRRTVEELTGRVTPDNRRAVLMIIDSYTKRITRLKQRIGEIDPQGYMQLQIDALNWEKEYVKARLAKVKATPNDDRAAHDLEIEACERLLDQIMSSLRHIETEHNSGRTIWRVKGRFRALQRRMSTMLKRVNSRIRRTTPLFSDDELFAHTRVVQVDAIEHVIDRLYEEMGRDTYNTEHCSALLLDYRRGEATLRSRPNVGTSAEAQTQAEDVKRESYGIELSVIQDMYEAGDITRAQSKQLRRNVYVMSVDADAQI
>NZ_AP012327.1|WP_033501959.1|2124371_2125106_+|esterase
MIGRESQVVPGRFGEPLQVTHVQYSRGGGNAAPRRPLFLCLHGWGSNEADLADMMRYVAPYNDFASLRAPLVLQEAGSGEFGFGQGAYSWFHDCVPSGEDLDRDAYAAAQAIDDWVARNVPEDRAVVPIGFSQGGLLAIQLLRVHPERYAASISLSGFLAPGIIAGSAPADSHVAELNIPVFYGYGKNDTVIPMPELFATAAWLDEHTFLTSKSYRGLDHAVSLNEFSDLRDWLAAHDIAPGIL
>NZ_AP012327.1|WP_004223871.1|2125338_2125920_+|dCTP-deaminase
MLLSDRDILAAQAEGHISLDPWTPEMVQPASIDVRLDRFFRLFNNHAYTYVDPAENQGELTEQFEVAPGEPWILHPGEFALGSTWEYVKLDPTIAARLEGKSSLGRLGILTHSTAGFIDPGFEGHITLELSNVSTLPVKLWPGMKIGQMCFFQLSSPAEHPYGSQGTGSHYQGQRGPTPSRSYVNFYKADITD
>NZ_AP012327.1|WP_128832406.1|2126162_2126285_+|hypothetical-protein
MKTESVYLGHWKECTRDEVLVLIDDYIHWHDHERIKQSLS
>NZ_AP012327.1|WP_051920323.1|2126474_2127350_+|aminotransferase-class-III-fold-pyridoxal-phosphate-dependent-enzyme
MAEKTTILDMNRFDDSNLDGLDENKRELVRRRRILGKGYRLFYKDPVEIVRGKGSHVFDEKGNDYLDAYNNVVSVGHCHPHVVEAIAKQAATLNTHTRYLHETILDYSEALIAKMPREIDRITYQCTGSEANDLAIRIARNYTGGRGIIITNEAYHGNTEKKPDEPGDPWFNFHAGRNDFKLRYALQGTRYWGSYITDAIKDYQETNSGEVEKTLRKDPKRVDENLKGLREELELLGGKPVLVALGYAAEKNLRRMRSEGYEVVRILHPARYIGKKEYRDRVLEVLDNIRK
>NZ_AP012327.1|WP_033501963.1|2134297_2137294_-|cation-translocating-P-type-ATPase
MVDRKEDQSTQFRDTSSGNFEQAKNIATQTQVDPSLADAQTVAQSLGVDLNTGLSQAEAKRRLDKYGPNELASAPPVPKWKKFLEQFKDPLVYLLLAATGISLAAWFIERANAVPGAEGGEALPFDAIVIVLILIVNAVLGYIQESKAEAAVEALSSMTAPQTNVLRDGQIERINTVGVVPGDIIVLGEGDSVSADGRLFTAASLRIAEASLTGESVPVGKKTDTLAQAKALGDRANMVFNGTSVTQGTGRAIVTSTGMGTQVGKIADLLQATEDDETPLQKEMNYVSKILGIAVCIIAVVVLVALALTEGFQDVHDVIDSLLLAVSLAVAAVPEGLAAILTVVLALGVQRMAMHNAIVKKLHSVETLGSASVICSDKTGTLTRNEMTVERVVTPSGEVQLTGTGYAPEGRMVVDSQTMEYAQIREIIESEAVATLAVGALANDGELREVAASAGNAENVTWEAVGDPTEVSLIVAARKVKANRKYANYERVGEIPFTSERKRMSIVAQDNTDAGRLTVFSKGAPDVLLGYCSRIAVGGAVRPLTEGDRQQILATVEQLSSDAYRTLGQAYRPLGTASLAQVPGIMLNSAGHVADIAEQSDVLENDLIWVGMVGIIDPPRTEVRDSVAEAHRAGIRTVMITGDHPLTAARIATDLGIIDKDGKAMTGSQLDELPDEAAFDKVTSEVSVYARVAPEHKLKIVESLQRQGNIVAMTGDGVNDAPAVKTADIGVAMGITGTEVTKQSAKMILADDNFSTIVAAVREGRGIFNNIRKFLRYLLSSNVGEVFTVFGGVMLAGFLGITQPGSQGVIVPLLATQLLWINLLTDAAPALAMGVDPSTDDVMARKPRKLTDRVIDAEMWGDIIFIGVIMAAVTLIGMDMHLAGGLFTDRSVDAIGHDAQMTEARTMGFTILVFAQMLNAIASRSHLQSAFVGLFSNKWLWGALALSTVLQLAVIYIPFLNIAFGTVPLSAGAWVECLGLAMIVLIASELRKCVLRAR
>NZ_AP012327.1|WP_048349547.1|2137636_2138890_-|hypothetical-protein
MCNDVSVIASSDASNASRVLNLPEGGSVRLCGAMDVQRVTIGERTGLRPIRLPLSGLVQRSLYEYETVRTVVSGCSGVHLRVRTAADAIRLAVRAARVDYGELNSEFNAFAATVDGRTVCEVTSQPDAIERVSRDGRTCVRTELDECSVVEFTGLGAIGEKTVVIWLPQTVIVDLLGVSGVHGEPVEAAEESSTPRWLHYGSSISHCHTPSSPLQVWPVLAAGLNDLEVTNLGFAGQCMLDPYVAKAIAASDADVITLSVGVNITGARAMNQRTFTPALHGFLDVIRERHPDTPIVLVSSIYWPDSDDVPGPSDVRFHDDGSVTCYSYGDRKEIAVGALTLEQSREELQHVTQVRHDTYGENIQYLDGLTLFGPSDVGNFCLPDGLHPDAQLYAEIARRFSCQVFADNGLVPRASLH
>NZ_AP012327.1|WP_033501964.1|2138911_2139721_-|alpha/beta-hydrolase
MNLKPISFDVEGLTLRGSVYEPKGGFEQEKRYPTALLFHGFGGNRIDFSGFMMQMARSLSQQGIVAITYDRAGHGESDGTFFDVSISREVRHAKQVLQQVCSLDYVDENNLHFGGISLGAVVESIVASETAAKPRSMVMVSSAASCVDEITSGKIQGKSLEGLRTQSYFDFMGAKMGPAMVEDVKGLDLYAKVTGCTSDVLLIHGTADFVPVEYAQRYKDVFGDRATLIVKENGDHGFCSVPDREFVMSKTAQFIAEHAGVSYQGVEIF
>NZ_AP012327.1|WP_128832457.1|2139717_2140935_-|MFS-transporter
MATFVDNAATMGVSTAFVLYQSGGRLTGNQIGMLTSSVTIGVALGSFLGGRLGDRFGRRHVFVGTMLIIILGAFIPFISTDFAFMLPGVLLLGLGIGADLPVAMATISETATDKNRAKILVFSNLLGGFGILMSVLLGIFFGNSGVFGGHVIFGAFGGVAVIVLLMRLTIPETETWLKANEERKEGVHTLRADNSSVKDLMKTPWRKPFVTLVCYYSLVAISVSVSSGFGTYIAYNVANISVSTYSTASLACMPLAILGAAWFMAVADSKWRMTYYVIGSVLVAVSGLIPVIFGFNLVTLCAFTYIVTFAGAFCYETIMKVWTQESFPTMLRGTAQGFIYGISRVLTAIMSAVTPALLVFNPKMLYVSIALLSAVGFFVGWLGFHKSTGNTFQTEAQVESEGALQ

Crispr_ID: NZ_AP012327_5

• CRISPR_ID: NZ_AP012327_5
• CRISPR_location: 2257698-2257880
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 3 spacers

Consensus repeat of NZ_AP012327_5

Consensus_repeat	Method
ATTCCTCCAGTCAGTGGAGAAAT	CRISPRCasFinder

spacers of NZ_AP012327_5

>5.1|2257721|30|NZ_AP012327|CRISPRCasFinder
TACCCCGCTGACAAGCCCATCAACAACCCA
>5.2|2257774|30|NZ_AP012327|CRISPRCasFinder
TCTCCCGCTAACAGCCACCTCAGAGTCCAG
>5.3|2257827|31|NZ_AP012327|CRISPRCasFinder
CTGGACTGGAAAATACCCCCGTACTGCCCAA

CRISPR arrays and Neighbor proteins around NZ_AP012327_5

CRISPR arrays

The CRISPR arrays of NZ_AP012327_5

>merge|NZ_AP012327|5|2257698-2257880|CRISPRCasFinder
ATTCTTCCAGTCAGTGGAAGAATTACCCCGCTGACAAGCCCATCAACAACCCAATTCCTCCAGTCAGTGGAGAAATTCTCCCGCTAACAGCCACCTCAGAGTCCAGATTCCTCCAGTCAGTGGAGGAATCTGGACTGGAAAATACCCCCGTACTGCCCAAAACCTTCCAGCGAGTGGAGGAAT

>NZ_AP012327|5|3|2257698-2257880|CRISPRCasFinder
ATTCTTCCAGTCAGTGGAAGAAT	TACCCCGCTGACAAGCCCATCAACAACCCA
ATTCCTCCAGTCAGTGGAGAAAT	TCTCCCGCTAACAGCCACCTCAGAGTCCAG
ATTCCTCCAGTCAGTGGAGGAAT	CTGGACTGGAAAATACCCCCGTACTGCCCAA
AACCTTCCAGCGAGTGGAGGAAT

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
NZ_AP012327.1|WP_033501785.1|2255244_2256261_+|LacI-family-DNA-binding-transcriptional-regulator	unknown	unknown	gnl|CDD|380486
NZ_AP012327.1|WP_033501788.1|2246997_2248020_-|LacI-family-transcriptional-regulator	unknown	unknown	gnl|CDD|224525
NZ_AP012327.1|WP_033501777.1|2268775_2269402_+|hypothetical-protein	unknown	unknown	unknown
NZ_AP012327.1|WP_048349552.1|2248732_2250979_-|glycosyltransferase	unknown	unknown	gnl|CDD|379305
NZ_AP012327.1|WP_033501786.1|2252655_2255112_+|EAL-domain-containing-protein	unknown	unknown	gnl|CDD|376350
NZ_AP012327.1|WP_033501781.1|2263501_2264206_-|TENA/THI-4-family-protein	unknown	unknown	gnl|CDD|381693
NZ_AP012327.1|WP_033501790.1|2244337_2245276_-|sugar-ABC-transporter-permease	unknown	unknown	gnl|CDD|224096
NZ_AP012327.1|WP_048349590.1|2242191_2243322_-|DUF2961-domain-containing-protein	unknown	unknown	gnl|CDD|378582
NZ_AP012327.1|WP_048349553.1|2260940_2261882_-|ROK-family-protein	unknown	unknown	gnl|CDD|224851
NZ_AP012327.1|WP_033501778.1|2266697_2268560_-|methionine--tRNA-ligase	unknown	unknown	gnl|CDD|237029
NZ_AP012327.1|WP_033501787.1|2251233_2252547_+|carbohydrate-binding-protein	unknown	unknown	gnl|CDD|119360
NZ_AP012327.1|WP_033501776.1|2269841_2270825_-|16S-rRNA-(cytidine(1402)-2'-O)-methyltransferase	unknown	unknown	gnl|CDD|223390
NZ_AP012327.1|WP_033501814.1|2269469_2269724_+|hypothetical-protein	unknown	unknown	unknown
NZ_AP012327.1|WP_033501780.1|2264378_2266484_-|glycoside-hydrolase-family-2	unknown	unknown	gnl|CDD|225789
NZ_AP012327.1|WP_033501791.1|2243390_2244269_-|carbohydrate-ABC-transporter-permease	unknown	unknown	gnl|CDD|223472
NZ_AP012327.1|WP_033501782.1|2262363_2263488_+|ROK-family-protein	unknown	unknown	gnl|CDD|224851
NZ_AP012327.1|WP_128832412.1|2256340_2257615_-|N-acetylglucosamine-6-phosphate-deacetylase	unknown	unknown	gnl|CDD|238434
NZ_AP012327.1|WP_033501789.1|2245441_2246749_-|extracellular-solute-binding-protein	unknown	unknown	gnl|CDD|270303
NZ_AP012327.1|WP_033501783.1|2258849_2260862_-|family-20-glycosylhydrolase	unknown	unknown	gnl|CDD|119335
NZ_AP012327.1|WP_033501784.1|2257968_2258781_-|glucosamine-6-phosphate-deaminase	unknown	unknown	gnl|CDD|179028

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
NZ_AP012327.1|WP_033501785.1|2255244_2256261_+|LacI-family-DNA-binding-transcriptional-regulator	gnl|CDD|380486	cd01544, PBP1_GalR, ligand-binding domain of DNA transcription repressor GalR which is one of two regulatory proteins involved in galactose transport and metabolism. Ligand-binding domain of DNA transcription repressor GalR which is one of two regulatory proteins involved in galactose transport and metabolism. Transcription of the galactose regulon genes is regulated by Gal iso-repressor (GalS) and Gal repressor (GalR) in different ways, but both repressors recognize the same DNA binding site in the absence of D-galactose. GalR is a dimeric protein like GalS and is exclusively involved in the regulation of galactose permease, the low-affinity galactose transporter. GalS is involved in regulating expression of the high-affinity galactose transporter encoded by the mgl operon. GalS and GalR are members of the LacI-GalR family of transcription regulators and both contain the type 1 periplasmic binding protein-like fold. Hence, they are structurally homologous to the periplasmic sugar binding of ABC-type transport systems.	3.53304e-81
NZ_AP012327.1|WP_033501788.1|2246997_2248020_-|LacI-family-transcriptional-regulator	gnl|CDD|224525	COG1609, PurR, Transcriptional regulators [Transcription].	9.79771e-54
NZ_AP012327.1|WP_048349552.1|2248732_2250979_-|glycosyltransferase	gnl|CDD|379305	pfam13632, Glyco_trans_2_3, Glycosyl transferase family group 2. Members of this family of prokaryotic proteins include putative glucosyltransferases, which are involved in bacterial capsule biosynthesis.	1.47307e-24
NZ_AP012327.1|WP_033501786.1|2252655_2255112_+|EAL-domain-containing-protein	gnl|CDD|376350	pfam00563, EAL, EAL domain. This domain is found in diverse bacterial signaling proteins. It is called EAL after its conserved residues. The EAL domain is a good candidate for a diguanylate phosphodiesterase function. The domain contains many conserved acidic residues that could participate in metal binding and might form the phosphodiesterase active site.	1.68548e-25
NZ_AP012327.1|WP_033501781.1|2263501_2264206_-|TENA/THI-4-family-protein	gnl|CDD|381693	cd19358, TenA_E_Spr0628-like, TenA_E proteins similar to Streptococcus pneumoniae Spr0628 and Saccharomyces cerevisiae S288C PET18. This family of TenA proteins belongs to the TenA_E class, and lacks the conserved active site Cys residue of the TenA_C class; most have a pair of structurally conserved Glu residues in the active site. TenA_C proteins (EC 3.5.99.2; aminopyrimidine aminohydrolase, also known as thiaminase II) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway; the role of TenA_E proteins is less clear. Arabidopsis thaliana TenA_E (not belonging to this family) hydrolyzes amino-HMP to AMP, and the N-formyl derivative of amino-HMP to amino-HMP. Members of this family include the putative thiaminase Streptococcus pneumoniae Spr0628, and Saccharomyces cerevisiae S288C PET18, a protein of unknown function whose expression is induced in the absence of thiamin. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. Many proteins in this family have yet to be characterized.	4.13002e-66
NZ_AP012327.1|WP_033501790.1|2244337_2245276_-|sugar-ABC-transporter-permease	gnl|CDD|224096	COG1175, UgpA, ABC-type sugar transport systems, permease components [Carbohydrate transport and metabolism].	7.06195e-78
NZ_AP012327.1|WP_048349590.1|2242191_2243322_-|DUF2961-domain-containing-protein	gnl|CDD|378582	pfam11175, DUF2961, Protein of unknown function (DUF2961). This family of proteins has no known function.	6.83468e-115
NZ_AP012327.1|WP_048349553.1|2260940_2261882_-|ROK-family-protein	gnl|CDD|224851	COG1940, NagC, Transcriptional regulator/sugar kinase [Transcription / Carbohydrate transport and metabolism].	2.08452e-48
NZ_AP012327.1|WP_033501778.1|2266697_2268560_-|methionine--tRNA-ligase	gnl|CDD|237029	PRK12268, PRK12268, methionyl-tRNA synthetase; Reviewed.	0
NZ_AP012327.1|WP_033501787.1|2251233_2252547_+|carbohydrate-binding-protein	gnl|CDD|119360	cd06543, GH18_PF-ChiA-like, PF-ChiA is an uncharacterized chitinase found in the hyperthermophilic archaeon Pyrococcus furiosus with a glycosyl hydrolase family 18 (GH18) catalytic domain as well as a cellulose-binding domain. Members of this domain family are found not only in archaea but also in eukaryotes and prokaryotes. PF-ChiA exhibits hydrolytic activity toward both colloidal and crystalline (beta/alpha) chitins at high temperature.	1.26471e-92
NZ_AP012327.1|WP_033501776.1|2269841_2270825_-|16S-rRNA-(cytidine(1402)-2'-O)-methyltransferase	gnl|CDD|223390	COG0313, COG0313, Predicted methyltransferases [General function prediction only].	4.08958e-103
NZ_AP012327.1|WP_033501780.1|2264378_2266484_-|glycoside-hydrolase-family-2	gnl|CDD|225789	COG3250, LacZ, Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].	1.87079e-34
NZ_AP012327.1|WP_033501791.1|2243390_2244269_-|carbohydrate-ABC-transporter-permease	gnl|CDD|223472	COG0395, UgpE, ABC-type sugar transport system, permease component [Carbohydrate transport and metabolism].	7.87792e-65
NZ_AP012327.1|WP_033501782.1|2262363_2263488_+|ROK-family-protein	gnl|CDD|224851	COG1940, NagC, Transcriptional regulator/sugar kinase [Transcription / Carbohydrate transport and metabolism].	3.61257e-37
NZ_AP012327.1|WP_128832412.1|2256340_2257615_-|N-acetylglucosamine-6-phosphate-deacetylase	gnl|CDD|238434	cd00854, NagA, N-acetylglucosamine-6-phosphate deacetylase, NagA, catalyzes the hydrolysis of the N-acetyl group of N-acetyl-glucosamine-6-phosphate (GlcNAc-6-P) to glucosamine 6-phosphate and acetate. This is the first committed step in the biosynthetic pathway to amino-sugar-nucleotides, which is needed for cell wall peptidoglycan and teichoic acid biosynthesis. Deacetylation of N-acetylglucosamine is also important in lipopolysaccharide synthesis and cell wall recycling.	5.025e-128
NZ_AP012327.1|WP_033501789.1|2245441_2246749_-|extracellular-solute-binding-protein	gnl|CDD|270303	cd13585, PBP2_TMBP_like, The periplasmic-binding component of ABC transport systems specific for trehalose/maltose and similar oligosaccharides; possess type 2 periplasmic binding fold. This family includes the periplasmic trehalose/maltose-binding component of an ABC transport system and related proteins from archaea and bacteria. Members of this group belong to the type 2 periplasmic-binding fold superfamily. PBP2 is comprised of two globular subdomains connected by a flexible hinge and bind their ligand in the cleft between these domains in a manner resembling a Venus flytrap. The majority of PBP2 proteins function in the uptake of small soluble substrates in eubacteria and archaea. After binding their specific ligand with high affinity, they can interact with a cognate membrane transport complex comprised of two integral membrane domains and two cytoplasmically located ATPase domains. This interaction triggers the ligand translocation across the cytoplasmic membrane energized by ATP hydrolysis.	1.08882e-35
NZ_AP012327.1|WP_033501783.1|2258849_2260862_-|family-20-glycosylhydrolase	gnl|CDD|119335	cd06565, GH20_GcnA-like, Glycosyl hydrolase family 20 (GH20) catalytic domain of N-acetyl-beta-D-glucosaminidase (GcnA, also known as BhsA) and related proteins. GcnA is an exoglucosidase which cleaves N-acetyl-beta-D-galactosamine (NAG) and N-acetyl-beta-D-galactosamine residues from 4-methylumbelliferylated (4MU) substrates, as well as cleaving NAG from chito-oligosaccharides (i.e. NAG polymers). In contrast, sulfated forms of the substrate are unable to be cleaved and act instead as mild competitive inhibitors. Additionally, the enzyme is known to be poisoned by several first-row transition metals as well as by mercury. GcnA forms a homodimer with subunits comprised of three domains, an N-terminal zincin-like domain, this central catalytic GH20 domain, and a C-terminal alpha helical domain. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by solvent or the enzyme, but by the substrate itself.	6.79505e-91
NZ_AP012327.1|WP_033501784.1|2257968_2258781_-|glucosamine-6-phosphate-deaminase	gnl|CDD|179028	PRK00443, nagB, glucosamine-6-phosphate deaminase; Provisional.	1.20565e-122

Protein sequences

>NZ_AP012327.1|WP_128832412.1|2256340_2257615_-|N-acetylglucosamine-6-phosphate-deacetylase
MDVALAQSARPFAIHNARKVDARGVAEHYWLVSDGSAIIATGNRDDDFATACASVGLDADADSDSVSSVGSGDSMTGSVGSVTNAAGRMMTPGYVDIHAHGSWGSSFDDGVQGIRTARAGHMMHGTTRQVLSLITNPLDVMCANLQTVRGMMNARPDILGAHLEGPFLALSRKGAHDPECLKDPVPEYVDRLLQAADGCLRQITIAPELPHGIYAIRRFAAAGVVPAVGHCDADYATARKGFNAGAGIMTHMFNAMNGLHHREPGPIPAAVEDPRVTVELINDGFHVQDPMVRLGFGFAPHRIAFVTDAMAATDCPDGHYLLGALDVNVIDGHARLASNGAIAGSTLTLEKAVQRAVLELGFTPTDAVEAATLTPAKAFGFDRANPVTKQPLGLLAPGYAADVLLLNPATWAVEHVWCDAHLLR
>NZ_AP012327.1|WP_033501785.1|2255244_2256261_+|LacI-family-DNA-binding-transcriptional-regulator
MSTIREIAKRVGFSQATVSRVLKDDPTFSVKDSTREKILNASLEMGYKNVPQYQRITIPQNVAILDNVVPDEGLQDAYFDELREVLVKHAEEQLMNVTMQKDVDSLIANADKYAGFISIGPSPLDRKTLHRLHKKLPHGVFIDINPAPALFDSVQPDLEQTILDAIDALMASGYSRIGFIGGLGNIMGEHEYPEDVRTFAFRNWALRLGLDVKGLVYADGPFTVENGRLQGRQLVQDHADDLPDAVIVAADPLAVGVLQAFATENVMVPRDIKVISINNQEIAEYTSPALSSYDISQDELAKAAVLMLAEALTANRKISQHMRISTSLVVRDSFTPQD
>NZ_AP012327.1|WP_033501786.1|2252655_2255112_+|EAL-domain-containing-protein
MASKQSKHQATTGSHSRDGIRKPVRLTILAVIYTLFLAVAIAFHAQEGVQRLMEAPARICIWSLCFLLCLIYHKKRSSYRVAAEHTVLPPLTVILLVTGYFPNVAMFLIIVTIITSIITARSLKIGILHAGTTLAPIPLIKILFANTSHVLSIIIRQNSVNAAINPYQFQHWLLPLLIITAAVPIIRLICDIVTFFFVQIPIRKAMHEYSLSHILAMALADLMAIVWIPEILEFANIGSDTATVLSVFMLALIACSLLLMTVDTMSRLARSRSALKCIANVSDALPLPNQVPEETVVRRINRGLTRMRCFISNANNLDKRGYSYRYSAPISTGSRQYYLATERSIWNRPFMSTDETILLTCGEILTESLRVNKEVTLLRTESETDTLTGALTYRAFIGHLKSLQTENVHNLVAVVYFSVEHLRTVNEHYGRKIGNAVLRSVGMRLSQLLPNNVTLYRVNGAEFAMIVTDVTSTSDVEELATRMRNLAVMPVHTEEGEVSVDVSSSISFSNATDGFAVLLADASAHIYALESSNLPAVDGTTATLAAQNSSDDYVNASDVLRHAIEDNTISVLYQPIFDVNTKRITSLDTIVRVHDAKGRTLAPYFVTAEAHRLNMSVQLTLDVLETCVKDMTAFRQVAPELDIVDICMNGSELGASIFHERMEQLTHEQPQLRFGLQLGSHAIHVAHDEVDDEVADLAALPNVELGLTNAGTTYSEVAAFAHLPLDFVRFDKTVVRDFRTPRAKQIMQRTLDISRDNDAFHVVFDGVETLDQVEFIRSIGGTLAEGTLLSNAMSANEFLMRLETMGTALPEAAPRQAE
>NZ_AP012327.1|WP_033501787.1|2251233_2252547_+|carbohydrate-binding-protein
MMKPLFPGRRFSFLRLFIAILCIALVAAGTWSWITFTRTAAKKLPEPWFGGYVDVTTTPSYEFESKVGNVYRNVILGFVTAGDGCQPSWGGYYTLDEAASTLDLDSRIAQTYKTDRTVTVSFGGQNGTELAAACTDVDALANAYQQVIDRYHITSLDFDIENTNLDGYSETATRRAQAVVKLIANEKAKNKGKDDTSHDLIISLTLPADAKGLTAQGMQTVNAFLDAGVTLSTVNLMTMDFNVASTSITQSTLIKSSLNAAHAQYKTLLYSRGRLFSDHQIWELLGATVLIGQNDTKNEYFTLDNAREINTFALETSLGHLSMWSLNRDQQCGENYTNTNTLKTFCSGRKQTDGEFATTLGSGFRGTPGTLVDFDNTSWNSSQQAYPTWEPDVLYKQGDKVIWNGNIYESLGNNENEQPDSAEEGTNAPWRIIGPVL
>NZ_AP012327.1|WP_048349552.1|2248732_2250979_-|glycosyltransferase
MKIGGNEGGDRKHQWGAETSTQPLAIMHAKPSPTRVVLGYVAIACTVISWLVYMITMILSLFVNNPSLTLRFVVEGVLYMTIVTTLIFSALVYLTTRQAALYRFIRHERVPRAMLDDHFAHNYSKGITVLIPSYVEQPKVVEKTIWSAALQEFPDLAVVLLIDDPPHSKNDEARAILKASRELMPKVLAELAAPAERFTKARDETAATLANQMSARRSVVAHCAEDYRAAAQWLEHKADMWLIEDHTDDFFCDQVLRGLARDLRLTEQALNESITLQQHVDANRILQLYERLVRIFTAKGWSFERKLYASTSREGNKAMNLNSFIGLMGHSLKRVETSDGVILRDVREDESPDFVMRNSEYVLTLDADSMLLRDYCLRLVYQMEQPGNERVAVIQTPYSSYRGAPTRIERIAAATTDIQHMLHQGMTYYDATFWVGANAVIRKAALDDICVVSTEGTRTVKTYIQDRTVIEDTESSIDLGYFGWHLVNYPERLSYSATPPDFGSLVVQRRRWANGGLLIIGKFFRIVHARHQQGNDVTLGEWALRVNYMASIAWSSFGLMFLLAYPFDQRLLSPWVVFASLPYFATMSSDLKRNGYKRSDIFRIYGFNIVLLTVNLSGTLKSIQQGMTNTKIPFARTPKVNNRTASPALYVTMPWVIIIYSCMVFYADTFSHNWGNAVFAGFNAIVTFWALTAYIGIWHSVQDMVVGLIRWFFVPIKEPQQEAARKKSSWRDVLYFGDRQMIYESRPL
>NZ_AP012327.1|WP_033501788.1|2246997_2248020_-|LacI-family-transcriptional-regulator
MAGIKDVAQEAGVSVSTVSYALSGKRSISAKTTDKVMAAVERLGYTPDASARKMRGMRNHIFALSAPIRGDINQAKYNAYFLQTAWQAKNRGYDVLLLTGEDAVADIRRVTQSNLVDGVVLLDIEERDERTAQAGSYSKPCVAIGYPAEHEGCACVDIDFAEAGRMAADCLYSKGHRNAMFLRDNETDYDRGSGYVLLFREALLERAKELGMTIHESSKHRDDRFDAAEFVRELRSSGERPTAIVNQANANVLKLVLQQLQASELRVPDDISVLSCGTYFEGELMPQPITEMPVMPQELCSKAVDLLVEAIDEHRDIKGLVELSTPVMHSRGSVTKVGDA
>NZ_AP012327.1|WP_033501789.1|2245441_2246749_-|extracellular-solute-binding-protein
MKITQGIALAAAAAMSLATLAACGSDTAQDAETPDSLSFWYYEEDDAGQTQAWRRAAEAFEKETGVEIKFERKSFTQIAQNGSQFLNSDEAPDLMESNRGNGSAGVLSTMGLLTDLGDYVSQYGWDKKVTGANAAVAKYDENGIMDGDTWYGMTSYAEFQRVYYNKDLFAKYGLEIPTTYDEFVEVCQKFVDAGVTPIAADAQEYGVMWLWWQLVSKNADAKFIDNWQLYKGDVDWNSKILTNSVNTINDWLDKGFISRNVTGMKAEDTTQAFIKGEYPIYQTGTWNQGRFVKQITTFDWDAAVMPESNFAIGCAGNLLVIPERSHHKDLAAKFIDYVLSDDVQNYLGNAGGIPVAGDVSKITDEKSKDMIEEYTSYANDGKLSYYPDYAASNLTDAVPAEFQELVNGTKKPADVLKGIHEKYDVGVEDMGVKTN
>NZ_AP012327.1|WP_033501790.1|2244337_2245276_-|sugar-ABC-transporter-permease
MSKKTKTGGVSMSRLPGNRSAKFVPYLIPGLVGLLVIVVIPFVWNIYLSFTRWRGVGPVKFVGLQNWKRLFADSTFWISFANSFWIIVAIVVIPTILGLFISSLLTDVIQKKFGGKTASFLRAMFYLPQLLPVAVAAIIMGWIFRPEDGAVNALLVKLGLGSLQHNWLGSPDSALPVLMFILVWIQLGYPIVIFMSGLQRVDPELYEAAGLDGANWWQKFRVVTLPSIIPELLVVILTATIGALKTFAPVYLLTKGGPGTATTVPSYYSYNQFFQVQQVGYGAAISTALTVVIIVFSIVFTMVQKHVEKELV
>NZ_AP012327.1|WP_033501791.1|2243390_2244269_-|carbohydrate-ABC-transporter-permease
MTQTMTASAARKASKKTQHVRSTGDWITLIVLIAAALLVLFPLLVLTINAFKTPVDYNATGPLSLPKHFTMDGIISFWTTTKFPLKFWNSLIISLVVAAAAVVLSVLNSFALGIGRVKGNTWIVLAIMLANMMPQEALLYPLYTMFKQLGLYNTKLAIIIVFTIIQSAYGTYLLSSVYGTFPQAILEAAAIDGASRWQILRKVVLPISWPTISVLFVFFFVWTWNEYMIPMAFLMGDDVQTIPLALATLQGQRTMDATTLASASLLSIVPTIIFFIIFQRKLSQGITAGAVK
>NZ_AP012327.1|WP_048349590.1|2242191_2243322_-|DUF2961-domain-containing-protein
MQVDSLGAFSARNPLDSLAVAAPGRTRCVNAENPTGGKGTAATAASALGPSRKGSPCIQTVKAGENVTLMDVDGPGVIRHIWMTVTDRTSPTGPNVLRNLILEFYWDGEETPSVQCPIGDFFCCGHAQACRVNSMPVVVVPNRGFNCYFSMPFEHARIVLRNDHNEDVPAFFYQIDYTEYDALPADTMRFHAQWRRERVTELARDYVVLDGVHGRGAYIGTYLALTALESRWWGEGEVKMYIDGDDQYPTWCSTGAEDYFGGAWSFADFDEHGRMHEQTFCAPYVGFPFYSQRLASHRESAYWDVNTPVTRGLYRWHIPDPIYFEHDLRVEWQQIGTEEGGNFERQDDVASVAYWYQLEPHTPFTPIGDRHFRQPR
>NZ_AP012327.1|WP_033501784.1|2257968_2258781_-|glucosamine-6-phosphate-deaminase
MTEVIIVKNEREAGDIYGRSVADLIKTKPNAVLGLATGSSPLAAYQSLARIVHSEHIDVSQVRGFALDEYLGLPLDHPQSYHSTIHRTVVEPLGLNPDLVHVPSDVLDGAPLSDGERIAAAGPAYDRAIEEAGGIDVQILGIGTDGHVGFNEPGSSLASGTRVKTLVEQTRIDNARFFDDDINKVPTHCITQGIGTILKARHLVLLAFGSGKAEAVAETVEGGVSAFCPASALQMHPHATIIVDEEAASRLRHKDYYRYAYAHKPSWQHI
>NZ_AP012327.1|WP_033501783.1|2258849_2260862_-|family-20-glycosylhydrolase
MTNENTREWAIIPEPNSMTVGEGAVSLPYAGRVNESIAIGDGDYLLARQLVAGIRSTTGLDWDYATGCMWPGFISLHIADSDASEESGTPGAYTLTIDTDGIAITGYDFEGVRNGVQTLRQLIGQNGAVLPVLQIEDAPAYQIRGYYLDATRGRVPTLAWLKQWADKLCLYKYNQLQLYIEHTFAFDGMSETWRGSSPLTPADILEFDQYCANLGIELVPSVSTFGHQYVAMRTQELRELGEFPEDADRPFSFIERMRHHTLNVADDRSFAFSTQLVDSYLQLFRTKRFNICADETFDLGKGQSKQEAQRVGVATLYATYVGKLCEHLSQQGHEPMFWGDIAIEMPEILETLPNNVTLLNWQYEPKATDEKIQLVAKAGAKQIVCPAVWGWNALLPRVDDAWNNIARIARYGRDCGAEGMLVTDWGDFGHVNDPRMAVPGMIFGAQYAWNPTENIAGDTDENSAENDLLARISRVEYDDCSARFVTLLRSASAQAVFTWRELVEYLELDDGTGNCNTDVAQTIPCLTERLANPGNALTLREARDRMMRNMATTIERIPQANRKLQQCMVEIAPIMAHIGKPGIAAAVRIAMEGQLLLNRVGFALAGAHDVRDAHDVDDQQPSDCCALARDLECWCEAYAKEWAVTSQPSELHRIQETVWKAADELRRISK
>NZ_AP012327.1|WP_048349553.1|2260940_2261882_-|ROK-family-protein
MNARLHHNNRPQGCIGIDIGGTKIEGVLLDATDRVVDVCRMPSHSGENNVVTDIVRVARTLSNEPLPIGIGIPGQVNCATGQVRNVVNLGIETLELGERISEIMHVPVHVENDVNAAAVGATEFVEHVDGNATVVFLNFGTGLAAGLVRDGQAEHGYSGCIGEIGHLPVDPNGFACPCGQRGCLETVASGGAVAKLWPSANPPMPDLIRKARQGDEHANDVLGKVAHGMGDVIQIVAQAYDPQRIIIGGGMAKTGNDLIEVIQAELSRRAEGCRFLNTLDIASKIRIAAVDKPIGAIGAALAAKRAQLGNQTM
>NZ_AP012327.1|WP_033501782.1|2262363_2263488_+|ROK-family-protein
MSGTQAKASPSDIRISNITAAFEQLFPATQMSRANIGKLIGLSRFTTSEVTGEMVDNRILRELGPDNREGRGKRSQVLAIDTDFWRIVSIDLSNPKLAKGALADLSGRIVDRTELPADNGCSVDDVIGLCKQLIASTPLPILGIGIAVTGIVEPDGVVRKSVHLEWNELPLKAEVENATGVPTLVGNDTNAALVAERFFGDCSPNSMLISIGRGVGAALCLNDVIIEGSSSTAGEIAHVVVDPNGPTCECGKRGCLESLVSDDRLQERIAQDPASRTRILAEAGALLGRVMAIPTGLLDLRDISIYGAPEIVNAQFIDAMEDEIRRTMTTDYQKAPQIHRCRQGEDLTLRGQATIVIRTLVPTIHERGSLIGDA
>NZ_AP012327.1|WP_033501781.1|2263501_2264206_-|TENA/THI-4-family-protein
MTVNHVPEAPQSASGDYAWLGAEPGSVADQLYMSAADEWNQAINSRFVNEMLDDTLPESILKSYLIQDFKFFNQGIMSHAIELAPRQETKDMLAKQSQWFADNEATYFTGFLKEYGITDEEYNNSEQTPANREYCEYLTKLVQGTWEELITALCCMEWIYLAWAKRTIDAGVIQQIPAHKGWVDLHEGDHFRAWTGRLIALVNEYASVDGSEAEVFRTIVHLERRFFEDSYPQQ
>NZ_AP012327.1|WP_033501780.1|2264378_2266484_-|glycoside-hydrolase-family-2
MLDVKRVLKAAPTKMRSQEPLQQLWTPWGEQIASEWLSSNAAHKAFCPAKDGERKLKNGRTPILNEHPRPTMMRSNHTMLNGFWDYAIVSIPESDNGPKCRKSATALTRDEAVQAVKETAIPETFDGKILVPFSPESALSGVHQTIYPDNMIWYRTIVHPVTLGGEEAVRQIGDANRLILHFEAVDYVCACFINGQFAGVHVGGYLPFDVDISAFVDPDDDFEIALCVYDPNDSGTQMRGKQKIEREGIWYTAQSGIWQSVWMEIVPEAYIQTLTLKGAADGRLFIRAEIGGDKPNAKLHIVVTDSADGTIVADERFAAGMRKVRTEIATRAEHLWSPEDPYLYDVTAMLEFGAAKSQEKQDVVRSYCAFRTVEIKPDLKGVARFHLNGKPLFLKGVLDQGYWPDGLLTAPSDEALIHDITAMKESGFNMLRKHIKIESARWYYHCDRLGMLVWQDAVSGGGEYNAWTTNRKPTLVRTTWNKYRDDTAKHFAALGADDSTYRRDWSRTCDAMVHMLAGHPSIAAWVLFNEGWGQFNACDAAERIHALDPTRPIDATSGWYDQRCGDFHSVHNYFRPLEIYPDKGPLRGYVAEYEKRHKRRRRAAHYAVLPVAQHGVRAFMISEFGGLAQLVADHAAVSRAYGYGEYDSIEDWRAAVRSVLASAESLESRGLAGYVYTQVSDVEEELNGLLTYDRRLNKFVQ
>NZ_AP012327.1|WP_033501778.1|2266697_2268560_-|methionine--tRNA-ligase
MSHILVNVAWPYANGPRHIGHVAGFGVPSDVYARYERMKGNDVLMVSGTDEHGTPILVEADKEGVSAQELANRYNRVIAKDLCDLGLSYDLFTRTTTGNHEQVVQELFKQCLANGYIYKGTQKVAISPSTGRTLPDRYIEGTCPICGADGARGDQCDACGNELDPDELLNPVSKINGETPRFEETEHFFLDLPALAEANLAWLKTREGWRTNVINFSIGLFKEVKPRAITRDIDWGIPVPVAGWIDNPNKKLYVWFDAVIGYLSASIEWARRKGDPEAWRAWWNDPETPGYYFMGKDNITFHSQIWPSEMLGYNGQGSKGGETGKLGPLNMPEQVVASEFMTMEGKKFSSSRGIVIYVKDILARYPVDAVRYYISVAGPESSDSDFTWAEFVRHNNEELAASWGNLVNRVANLMNKNFGEIPALDENELTDEDRALLSETKAAFNTVGSLIENHRQKNALSEAMRVVGDINKYISATEPWKIKDNPARLGTVLHVAAQAVSDANHLLAPFLPHSAQKVWEALGGTGTFSPLPRLEEVEDLDKPGFMYPIITGDYKLGETVHPWASEPIVAGAPVPKPHPIFAKIPPEAVEEELARFDSELKARREAEAARLAAEKAKLEG
>NZ_AP012327.1|WP_033501777.1|2268775_2269402_+|hypothetical-protein
MDTSPPLLGNADLPGPMCLNLLASSGALCKLDSANAYSSHDSDTLYGRACIIATLLPSRSVACARTAAWVWLGGTFPDSIDIIAKAHYRTLRYGRKINVFNRVAPSEHTIKVGPITVTTPVRTACDLALNCVPATESKVSEIICMLMQEFHFRSNDCMQIMEDAKHIRNAPQARNYILAIDGSSYEHGNRKDCEDRKNRKDAEYVRGA
>NZ_AP012327.1|WP_033501814.1|2269469_2269724_+|hypothetical-protein
MEPPSPPILLTPLVACSPGTAQDVLWHIAEYAPRLRKWLIANPSATPAMLEYLAQVGGPDVARSLQILLESLESRALDAIAHDG
>NZ_AP012327.1|WP_033501776.1|2269841_2270825_-|16S-rRNA-(cytidine(1402)-2'-O)-methyltransferase
MERMNERECEDKREREDENADGDVGLDALVSGANIPHGTVVLAATPIGNTADASARLIALLERADIVAAEDTRRLYALANRLGVHVNGRVVAYHDHNERDKSDGLLDQVETGATVLVVSDAGMPTINDPGLAIVRRAIERGLPVTCAPGPSAVLDALCLSGLPTDRFCYEGFLPRKHSERVQYLRTLRSERRTIVFYETLHRIDEAMADLLDVFGPNRKMALCRELTKDFEQIRRGTIAQIRQSVIDEPPRGEMVLVVAGASEEEADAAVPASLSIEDLAVLSIDRAREDNLRIKDAISQVIAEHPLADGSLANRKQVYNTVLAMKE

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity
NZ_AP012327_2	2.1|360500|29|NZ_AP012327|CRT	360500-360528	29	NZ_CP032685	Rhizobium sp. CCGE531 plasmid pRCCGE531d, complete sequence	360455-360483	5	0.828
NZ_AP012327_2	2.1|360500|29|NZ_AP012327|CRT	360500-360528	29	NZ_CP032690	Rhizobium sp. CCGE532 plasmid pRCCGE532d, complete sequence	360454-360482	5	0.828
NZ_AP012327_2	2.1|360500|29|NZ_AP012327|CRT	360500-360528	29	NZ_CP018385	Burkholderia pseudomallei strain 2008724860 plasmid p1, complete sequence	286054-286082	5	0.828
NZ_AP012327_2	2.1|360500|29|NZ_AP012327|CRT	360500-360528	29	CP000619	Burkholderia vietnamiensis G4 plasmid pBVIE03, complete sequence	167204-167232	5	0.828
NZ_AP012327_2	2.1|360500|29|NZ_AP012327|CRT	360500-360528	29	CP000617	Burkholderia vietnamiensis G4 plasmid pBVIE01, complete sequence	13935-13963	5	0.828
NZ_AP012327_2	2.1|360500|29|NZ_AP012327|CRT	360500-360528	29	NZ_CP039340	Ralstonia solanacearum strain UW386 plasmid pUW386, complete sequence	738081-738109	6	0.793
NZ_AP012327_2	2.1|360500|29|NZ_AP012327|CRT	360500-360528	29	NZ_CP022999	Rhizobium sp. 11515TR strain 10195 plasmid p11515TR-A, complete sequence	637092-637120	6	0.793
NZ_AP012327_2	2.2|360551|26|NZ_AP012327|CRT	360551-360576	26	NZ_CP022999	Rhizobium sp. 11515TR strain 10195 plasmid p11515TR-A, complete sequence	1295910-1295935	6	0.769
NZ_AP012327_2	2.3|360599|29|NZ_AP012327|CRT	360599-360627	29	NZ_CP022999	Rhizobium sp. 11515TR strain 10195 plasmid p11515TR-A, complete sequence	1295910-1295938	6	0.793
NZ_AP012327_2	2.5|360692|26|NZ_AP012327|CRT	360692-360717	26	NZ_CP022999	Rhizobium sp. 11515TR strain 10195 plasmid p11515TR-A, complete sequence	1295910-1295935	6	0.769
NZ_AP012327_1	1.2|277008|28|NZ_AP012327|CRISPRCasFinder	277008-277035	28	NZ_AP022578	Mycolicibacterium aubagnense strain JCM 15296 plasmid pJCM15296, complete sequence	15399-15426	7	0.75
NZ_AP012327_1	1.2|277008|28|NZ_AP012327|CRISPRCasFinder	277008-277035	28	NZ_AP022578	Mycolicibacterium aubagnense strain JCM 15296 plasmid pJCM15296, complete sequence	252250-252277	7	0.75
NZ_AP012327_2	2.1|360500|29|NZ_AP012327|CRT	360500-360528	29	NC_024970	Streptomyces aureofaciens strain CCM3239 plasmid pSA3239, complete sequence	113987-114015	7	0.759
NZ_AP012327_2	2.1|360500|29|NZ_AP012327|CRT	360500-360528	29	NZ_KJ396772	Streptomyces lavendulae subsp. lavendulae strain CCM3239 plasmid pSA3239, complete sequence	113987-114015	7	0.759
NZ_AP012327_2	2.1|360500|29|NZ_AP012327|CRT	360500-360528	29	NZ_CP015032	Burkholderia cenocepacia strain 842 plasmid pBcn842-1, complete sequence	109827-109855	7	0.759
NZ_AP012327_2	2.1|360500|29|NZ_AP012327|CRT	360500-360528	29	NZ_AP022593	Mycolicibacterium arabiense strain JCM 18538 plasmid pJCM18538, complete sequence	1879440-1879468	7	0.759
NZ_AP012327_2	2.1|360500|29|NZ_AP012327|CRT	360500-360528	29	NZ_AP022593	Mycolicibacterium arabiense strain JCM 18538 plasmid pJCM18538, complete sequence	5215291-5215319	7	0.759
NZ_AP012327_2	2.1|360500|29|NZ_AP012327|CRT	360500-360528	29	NZ_CP024986	Streptomyces lavendulae subsp. lavendulae strain CCM 3239 plasmid pSA3239, complete sequence	127062-127090	7	0.759
NZ_AP012327_2	2.1|360500|29|NZ_AP012327|CRT	360500-360528	29	NZ_CP016083	Streptomyces sp. SAT1 plasmid unnamed3, complete sequence	793769-793797	7	0.759
NZ_AP012327_2	2.1|360500|29|NZ_AP012327|CRT	360500-360528	29	NZ_AP014810	Methylorubrum populi strain P-1M plasmid pMPPM01, complete sequence	17084-17112	7	0.759
NZ_AP012327_2	2.1|360500|29|NZ_AP012327|CRT	360500-360528	29	NZ_AP019534	Enterobacter asburiae strain MRY18-106 plasmid pMRY18-106EAS_1, complete sequence	20870-20898	7	0.759
NZ_AP012327_2	2.1|360500|29|NZ_AP012327|CRT	360500-360528	29	MK460246	Mycobacterium phage Nibb, complete genome	51564-51592	7	0.759
NZ_AP012327_5	5.1|2257721|30|NZ_AP012327|CRISPRCasFinder	2257721-2257750	30	NZ_AF135182	Serratia entomophila strain A1MO2 plasmid pADAP, complete sequence	130487-130516	7	0.767
NZ_AP012327_5	5.1|2257721|30|NZ_AP012327|CRISPRCasFinder	2257721-2257750	30	NC_002523	Serratia entomophila plasmid pADAP, complete sequence	130049-130078	7	0.767
NZ_AP012327_5	5.1|2257721|30|NZ_AP012327|CRISPRCasFinder	2257721-2257750	30	NZ_CP030128	Indioceanicola profundi strain SCSIO 08040 plasmid unnamed2, complete sequence	446685-446714	8	0.733

1. spacer 2.1|360500|29|NZ_AP012327|CRT matches to NZ_CP032685 (Rhizobium sp. CCGE531 plasmid pRCCGE531d, complete sequence) position: , mismatch: 5, identity: 0.828

tcgcggcagccgtggccgtcgtgatgatc	CRISPR spacer
tcgcggcagccgttgccggcgtgatgcgg	Protospacer
************* **** *******   

2. spacer 2.1|360500|29|NZ_AP012327|CRT matches to NZ_CP032690 (Rhizobium sp. CCGE532 plasmid pRCCGE532d, complete sequence) position: , mismatch: 5, identity: 0.828

tcgcggcagccgtggccgtcgtgatgatc	CRISPR spacer
tcgcggcagccgttgccggcgtgatgcgg	Protospacer
************* **** *******   

3. spacer 2.1|360500|29|NZ_AP012327|CRT matches to NZ_CP018385 (Burkholderia pseudomallei strain 2008724860 plasmid p1, complete sequence) position: , mismatch: 5, identity: 0.828

tcgcggcagccgtggccgtcgtgatgatc	CRISPR spacer
tcgtcgatgccgtggccgtcgcgatgatc	Protospacer
***. *  *************.*******

4. spacer 2.1|360500|29|NZ_AP012327|CRT matches to CP000619 (Burkholderia vietnamiensis G4 plasmid pBVIE03, complete sequence) position: , mismatch: 5, identity: 0.828

tcgcggcagccgtggccgtcgtgatgatc	CRISPR spacer
tcgtcgatgccgtggccgtcgcgatgatc	Protospacer
***. *  *************.*******

5. spacer 2.1|360500|29|NZ_AP012327|CRT matches to CP000617 (Burkholderia vietnamiensis G4 plasmid pBVIE01, complete sequence) position: , mismatch: 5, identity: 0.828

tcgcggcagccgtggccgtcgtgatgatc	CRISPR spacer
tcgtcgatgccgtggccgtcgcgatgatc	Protospacer
***. *  *************.*******

6. spacer 2.1|360500|29|NZ_AP012327|CRT matches to NZ_CP039340 (Ralstonia solanacearum strain UW386 plasmid pUW386, complete sequence) position: , mismatch: 6, identity: 0.793

tcgcggcagccgtggccgtcgtgatgatc	CRISPR spacer
acgcggcagccgtgcccgtcgtgcccgtc	Protospacer
 ************* ******** . .**

7. spacer 2.1|360500|29|NZ_AP012327|CRT matches to NZ_CP022999 (Rhizobium sp. 11515TR strain 10195 plasmid p11515TR-A, complete sequence) position: , mismatch: 6, identity: 0.793

tcgcggcagccgtggccgtcgtgatgatc	CRISPR spacer
ttgcggcagccgtcgccggcgtgatgcgg	Protospacer
*.*********** **** *******   

8. spacer 2.2|360551|26|NZ_AP012327|CRT matches to NZ_CP022999 (Rhizobium sp. 11515TR strain 10195 plasmid p11515TR-A, complete sequence) position: , mismatch: 6, identity: 0.769

tgattatgatgatcgtccgcgtcgtc	CRISPR spacer
catgaatgatgagcgtccgcgtcgtc	Protospacer
..   ******* *************

9. spacer 2.3|360599|29|NZ_AP012327|CRT matches to NZ_CP022999 (Rhizobium sp. 11515TR strain 10195 plasmid p11515TR-A, complete sequence) position: , mismatch: 6, identity: 0.793

--ccgcgactatgatgatcgtccgcgtcgtc	CRISPR spacer
gttcatga--atgatgagcgtccgcgtcgtc	Protospacer
  .*..**  ******* *************

10. spacer 2.5|360692|26|NZ_AP012327|CRT matches to NZ_CP022999 (Rhizobium sp. 11515TR strain 10195 plasmid p11515TR-A, complete sequence) position: , mismatch: 6, identity: 0.769

tgattatgatgatcgtccgcgtcgtc	CRISPR spacer
catgaatgatgagcgtccgcgtcgtc	Protospacer
..   ******* *************

11. spacer 1.2|277008|28|NZ_AP012327|CRISPRCasFinder matches to NZ_AP022578 (Mycolicibacterium aubagnense strain JCM 15296 plasmid pJCM15296, complete sequence) position: , mismatch: 7, identity: 0.75

gttcgcaggctggttttgggtgtgtggc	CRISPR spacer
tgcagcaggctgattttgggcgtgtggg	Protospacer
  . ********.*******.****** 

12. spacer 1.2|277008|28|NZ_AP012327|CRISPRCasFinder matches to NZ_AP022578 (Mycolicibacterium aubagnense strain JCM 15296 plasmid pJCM15296, complete sequence) position: , mismatch: 7, identity: 0.75

gttcgcaggctggttttgggtgtgtggc	CRISPR spacer
tgcagcaggctgattttgggcgtgtggg	Protospacer
  . ********.*******.****** 

13. spacer 2.1|360500|29|NZ_AP012327|CRT matches to NC_024970 (Streptomyces aureofaciens strain CCM3239 plasmid pSA3239, complete sequence) position: , mismatch: 7, identity: 0.759

tcgcggcagccgtggccgtcgtgatgatc	CRISPR spacer
cggcggctgccgtgaccgtcgtgatgtgg	Protospacer
. ***** ******.***********   

14. spacer 2.1|360500|29|NZ_AP012327|CRT matches to NZ_KJ396772 (Streptomyces lavendulae subsp. lavendulae strain CCM3239 plasmid pSA3239, complete sequence) position: , mismatch: 7, identity: 0.759

tcgcggcagccgtggccgtcgtgatgatc	CRISPR spacer
cggcggctgccgtgaccgtcgtgatgtgg	Protospacer
. ***** ******.***********   

15. spacer 2.1|360500|29|NZ_AP012327|CRT matches to NZ_CP015032 (Burkholderia cenocepacia strain 842 plasmid pBcn842-1, complete sequence) position: , mismatch: 7, identity: 0.759

tcgcggcagccgtggccgtcgtgatgatc	CRISPR spacer
cgctcgcagccctggccggcgtgatgatc	Protospacer
.  . ****** ****** **********

16. spacer 2.1|360500|29|NZ_AP012327|CRT matches to NZ_AP022593 (Mycolicibacterium arabiense strain JCM 18538 plasmid pJCM18538, complete sequence) position: , mismatch: 7, identity: 0.759

tcgcggcagccgtggccgtcgtgatgatc	CRISPR spacer
gcgcagcagcggtggccgtcgtgatccag	Protospacer
 ***.***** **************    

17. spacer 2.1|360500|29|NZ_AP012327|CRT matches to NZ_AP022593 (Mycolicibacterium arabiense strain JCM 18538 plasmid pJCM18538, complete sequence) position: , mismatch: 7, identity: 0.759

tcgcggcagccgtggccgtcgtgatgatc	CRISPR spacer
cgaaggcagccgtggccgcggtgatgaac	Protospacer
. . **************. ******* *

18. spacer 2.1|360500|29|NZ_AP012327|CRT matches to NZ_CP024986 (Streptomyces lavendulae subsp. lavendulae strain CCM 3239 plasmid pSA3239, complete sequence) position: , mismatch: 7, identity: 0.759

tcgcggcagccgtggccgtcgtgatgatc	CRISPR spacer
cggcggctgccgtgaccgtcgtgatgtgg	Protospacer
. ***** ******.***********   

19. spacer 2.1|360500|29|NZ_AP012327|CRT matches to NZ_CP016083 (Streptomyces sp. SAT1 plasmid unnamed3, complete sequence) position: , mismatch: 7, identity: 0.759

tcgcggcagccgtggccgtcgtgatgatc	CRISPR spacer
cggcggcggccgtggccgtcgtggcgtac	Protospacer
. *****.***************..*  *

20. spacer 2.1|360500|29|NZ_AP012327|CRT matches to NZ_AP014810 (Methylorubrum populi strain P-1M plasmid pMPPM01, complete sequence) position: , mismatch: 7, identity: 0.759

tcgcggcagccgtggccgtcgtgatgatc	CRISPR spacer
tcgcggctgccgaggccgtcgtgggtggc	Protospacer
******* **** **********.  . *

21. spacer 2.1|360500|29|NZ_AP012327|CRT matches to NZ_AP019534 (Enterobacter asburiae strain MRY18-106 plasmid pMRY18-106EAS_1, complete sequence) position: , mismatch: 7, identity: 0.759

tcgcggcagccgtggccgtcgtgatgatc	CRISPR spacer
tactggcaggcgtggccgtcgtgattccc	Protospacer
*  .***** ***************  .*

22. spacer 2.1|360500|29|NZ_AP012327|CRT matches to MK460246 (Mycobacterium phage Nibb, complete genome) position: , mismatch: 7, identity: 0.759

tcgcggcagccgtggccgtcgtgatgatc	CRISPR spacer
tcgcggcggcggtggccgtcgtgccgcct	Protospacer
*******.** ************ .* ..

23. spacer 5.1|2257721|30|NZ_AP012327|CRISPRCasFinder matches to NZ_AF135182 (Serratia entomophila strain A1MO2 plasmid pADAP, complete sequence) position: , mismatch: 7, identity: 0.767

taccccgctgacaagcccatcaacaaccca	CRISPR spacer
gtcctctgtgccaagcccatcaacaacccg	Protospacer
  **.*  ** ******************.

24. spacer 5.1|2257721|30|NZ_AP012327|CRISPRCasFinder matches to NC_002523 (Serratia entomophila plasmid pADAP, complete sequence) position: , mismatch: 7, identity: 0.767

taccccgctgacaagcccatcaacaaccca	CRISPR spacer
gtcctctgtgccaagcccatcaacaacccg	Protospacer
  **.*  ** ******************.

25. spacer 5.1|2257721|30|NZ_AP012327|CRISPRCasFinder matches to NZ_CP030128 (Indioceanicola profundi strain SCSIO 08040 plasmid unnamed2, complete sequence) position: , mismatch: 8, identity: 0.733

taccccgctgacaagcccatcaacaaccca	CRISPR spacer
gaatccgctgacaagccgatcaacagcggc	Protospacer
 * .************* *******.*