CRISPRimmunity

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Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Self targeting spacer number	Target MGE spacer number	Prophage number	Anti-CRISPR protein number
NZ_CP017579	Sphingomonas melonis TY plasmid unnamed, complete sequence	0 crisprs	NA	0	0	0	0
NZ_CP017578	Sphingomonas melonis TY chromosome, complete genome	1 crisprs	DEDDh,csa3,RT	0	1	5	0

Results visualization

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CRISPR-Cas detection and classification

Crispr_ID: NZ_CP017578_1

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

NZ_CP017578_1

1262423-1262538

Orphan

Consensus_repeat	Method
TGCGGCGAGGGGAATGGCTGGAGGCTCGGACG	CRISPRCasFinder

1 spacers

csa3

The CRISPR arrays of NZ_CP017578_1

>merge|NZ_CP017578|1|1262423-1262538|CRISPRCasFinder
TGCGGCGAGGGGAATGGCTGGAGGCTCGGACGCTGCCCCTGCGGCTGGGTGGACCCCGGAACAAGTCCGGGGTGACGAGGGGTGTGCGGCGAGGGGGATGGCTGGAGGCTCGGACG

>NZ_CP017578|1|1|1262423-1262538|CRISPRCasFinder
TGCGGCGAGGGGAATGGCTGGAGGCTCGGACG	CTGCCCCTGCGGCTGGGTGGACCCCGGAACAAGTCCGGGGTGACGAGGGGTG
TGCGGCGAGGGGGATGGCTGGAGGCTCGGACG

Protein	Signature genes	Signature genes Name	Protein_function
NZ_CP017578.1\|WP_017977666.1\|1253604_1254651_+\|5-methyltetrahydrofolate--homocysteine-methyltransferase	unknown	unknown	gnl\|CDD\|236539
NZ_CP017578.1\|WP_018250505.1\|1269291_1270095_+\|transglutaminase-family-protein	unknown	unknown	gnl\|CDD\|224224
NZ_CP017578.1\|WP_018250504.1\|1272826_1273324_-\|hypothetical-protein	unknown	unknown	unknown
NZ_CP017578.1\|WP_017979475.1\|1273646_1274810_-\|IS110-family-transposase	unknown	unknown	gnl\|CDD\|226077
NZ_CP017578.1\|WP_017977665.1\|1252489_1253431_+\|methylenetetrahydrofolate-reductase-[NAD(P)H]	unknown	unknown	gnl\|CDD\|181852
NZ_CP017578.1\|WP_017977675.1\|1268353_1269292_+\|alpha-E-domain-containing-protein	unknown	unknown	gnl\|CDD\|377234
NZ_CP017578.1\|WP_024310446.1\|1271419_1272211_-\|arginyltransferase	unknown	unknown	gnl\|CDD\|234939
NZ_CP017578.1\|WP_017977663.1\|1251046_1251418_-\|hypothetical-protein	unknown	unknown	unknown
NZ_CP017578.1\|WP_017977664.1\|1251524_1252493_+\|metalloregulator-ArsR/SmtB-family-transcription-factor	csa3	COG0640_csa3_CAS-I-A	gnl\|CDD\|225136
NZ_CP017578.1\|WP_017977679.1\|1272465_1272714_+\|hypothetical-protein	unknown	unknown	unknown
NZ_CP017578.1\|WP_017977674.1\|1266666_1268166_+\|circularly-permuted-type-2-ATP-grasp-protein	unknown	unknown	gnl\|CDD\|225190
NZ_CP017578.1\|WP_017977670.1\|1260293_1261220_+\|hypothetical-protein	unknown	unknown	gnl\|CDD\|271210
NZ_CP017578.1\|WP_017977668.1\|1257842_1258697_+\|cupin-like-domain-containing-protein	unknown	unknown	gnl\|CDD\|372651
NZ_CP017578.1\|WP_017977671.1\|1261335_1262385_-\|exo-alpha-sialidase	unknown	unknown	gnl\|CDD\|372469
NZ_CP017578.1\|WP_017977677.1\|1270450_1271413_+\|cell-wall-hydrolase	unknown	unknown	gnl\|CDD\|226296
NZ_CP017578.1\|WP_018250506.1\|1264380_1266408_+\|hypothetical-protein	unknown	unknown	gnl\|CDD\|226685
NZ_CP017578.1\|WP_017977669.1\|1258882_1260091_-\|sodium/hydrogen-exchanger	unknown	unknown	gnl\|CDD\|223551
NZ_CP017578.1\|WP_017977672.1\|1262621_1264319_-\|alkaline-phosphatase-family-protein	unknown	unknown	gnl\|CDD\|293740
NZ_CP017578.1\|WP_081609112.1\|1254728_1257479_+\|methionine-synthase	unknown	unknown	gnl\|CDD\|236539
NZ_CP017578.1\|WP_017977662.1\|1250605_1250845_-\|hypothetical-protein	unknown	unknown	unknown

Protein	Function_ID	Function_description	E-value
NZ_CP017578.1\|WP_017977666.1\|1253604_1254651_+\|5-methyltetrahydrofolate--homocysteine-methyltransferase	gnl\|CDD\|236539	PRK09490, metH, B12-dependent methionine synthase; Provisional.	0
NZ_CP017578.1\|WP_018250505.1\|1269291_1270095_+\|transglutaminase-family-protein	gnl\|CDD\|224224	COG1305, COG1305, Transglutaminase-like enzymes, putative cysteine proteases [Amino acid transport and metabolism].	3.20745e-22
NZ_CP017578.1\|WP_017979475.1\|1273646_1274810_-\|IS110-family-transposase	gnl\|CDD\|226077	COG3547, COG3547, Transposase and inactivated derivatives [DNA replication, recombination, and repair].	9.82355e-33
NZ_CP017578.1\|WP_017977665.1\|1252489_1253431_+\|methylenetetrahydrofolate-reductase-[NAD(P)H]	gnl\|CDD\|181852	PRK09432, metF, methylenetetrahydrofolate reductase.	1.68634e-139
NZ_CP017578.1\|WP_017977675.1\|1268353_1269292_+\|alpha-E-domain-containing-protein	gnl\|CDD\|377234	pfam04168, Alpha-E, A predicted alpha-helical domain with a conserved ER motif. An uncharacterized alpha helical domain containing a highly conserved ER motif and typically found as a tandem duplication. Contextual analysis suggests that it functions in a distinct peptide synthesis/modification system comprising of a transglutaminase, a peptidase of the NTN-hydrolase superfamily, an active and inactive circularly permuted ATP-grasp domains and a transglutaminase fused N-terminal to a circularly permuted COOH-NH2 ligase domain.	3.86818e-86
NZ_CP017578.1\|WP_024310446.1\|1271419_1272211_-\|arginyltransferase	gnl\|CDD\|234939	PRK01305, PRK01305, arginyl-tRNA-protein transferase; Provisional.	9.70618e-127
NZ_CP017578.1\|WP_017977664.1\|1251524_1252493_+\|metalloregulator-ArsR/SmtB-family-transcription-factor	gnl\|CDD\|225136	COG2226, UbiE, Methylase involved in ubiquinone/menaquinone biosynthesis [Coenzyme metabolism].	1.51951e-22
NZ_CP017578.1\|WP_017977674.1\|1266666_1268166_+\|circularly-permuted-type-2-ATP-grasp-protein	gnl\|CDD\|225190	COG2308, COG2308, Uncharacterized conserved protein [Function unknown].	0
NZ_CP017578.1\|WP_017977670.1\|1260293_1261220_+\|hypothetical-protein	gnl\|CDD\|271210	cd14251, PL-6, Polysaccharide Lyase Family 6. Polysaccharide Lyase Family 6 is a family of beta-helical polysaccharide lyases. Members include alginate lyase (EC 4.2.2.3) and chondroitinase B (EC 4.2.2.19). Chondroitinase B is an enzyme that only cleaves the beta-(1,4)-linkage of dermatan sulfate (DS), leading to 4,5-unsaturated dermatan sulfate disaccharides as the product. DS is a highly sulfated, unbranched polysaccharide belonging to a family of glycosaminoglycans (GAGs) composed of alternating hexosamine (gluco- or galactosamine) and uronic acid (D-glucuronic or L-iduronic acid) moieties. DS contains alternating 1,4-beta-D-galactosamine (GalNac) and 1,3-alpha-L-iduronic acid units. The related chondroitin sulfate (CS) contains alternating GalNac and 1,3-beta-D-glucuronic acid units. Alginate lyases (known as either mannuronate (EC 4.2.2.3) or guluronate lyases (EC 4.2.2.11) catalyze the degradation of alginate, a copolymer of alpha-L-guluronate and its C5 epimer beta-D-mannuronate.	1.07494e-06
NZ_CP017578.1\|WP_017977668.1\|1257842_1258697_+\|cupin-like-domain-containing-protein	gnl\|CDD\|372651	pfam13621, Cupin_8, Cupin-like domain. This cupin like domain shares similarity to the JmjC domain.	8.84094e-37
NZ_CP017578.1\|WP_017977671.1\|1261335_1262385_-\|exo-alpha-sialidase	gnl\|CDD\|372469	pfam13088, BNR_2, BNR repeat-like domain. This family of proteins contains BNR-like repeats suggesting these proteins may act as sialidases.	5.5654e-90
NZ_CP017578.1\|WP_017977677.1\|1270450_1271413_+\|cell-wall-hydrolase	gnl\|CDD\|226296	COG3773, SleB, Cell wall hydrolyses involved in spore germination [Cell envelope biogenesis, outer membrane].	2.47539e-36
NZ_CP017578.1\|WP_017977669.1\|1258882_1260091_-\|sodium/hydrogen-exchanger	gnl\|CDD\|223551	COG0475, KefB, Kef-type K+ transport systems, membrane components [Inorganic ion transport and metabolism].	7.06605e-25
NZ_CP017578.1\|WP_018250506.1\|1264380_1266408_+\|hypothetical-protein	gnl\|CDD\|226685	COG4232, COG4232, Thiol:disulfide interchange protein [Posttranslational modification, protein turnover, chaperones / Energy production and conversion].	5.76149e-66
NZ_CP017578.1\|WP_017977672.1\|1262621_1264319_-\|alkaline-phosphatase-family-protein	gnl\|CDD\|293740	cd16016, AP-SPAP, SPAP is a subclass of alkaline phosphatase (AP). Alkaline phosphatases are non-specific phosphomonoesterases that catalyze the hydrolysis reaction via a phosphoseryl intermediate to produce inorganic phosphate and the corresponding alcohol, optimally at high pH. Alkaline phosphatase exists as a dimer, each monomer binding 2 zinc atoms and one magnesium atom, which are essential for enzymatic activity. Although SPAP is a subclass of alkaline phosphatase, SPAP has many differences from other APs: 1) the catalytic residue is a threonine instead of serine, 2) there is no binding pocket for the third metal ion, and 3) the arginine residue forming bidentate hydrogen bonding is deleted in SPAP. A lysine and an asparagine residue, recruited together for the first time into the active site, bind the substrate phosphoryl group in a manner not observed before in any other AP.	3.09846e-163
NZ_CP017578.1\|WP_081609112.1\|1254728_1257479_+\|methionine-synthase	gnl\|CDD\|236539	PRK09490, metH, B12-dependent methionine synthase; Provisional.	0

>NZ_CP017578.1|WP_017977671.1|1261335_1262385_-|exo-alpha-sialidase
MGWLALVITAAATPALAQSPILQSQFIDPAPPYPQAHASTIVELPDGQLAAAWFGGSGESRPDVKIWFARHSAQGWDTPVAVADGRGADGKLYPTWNPVLFQPAGQPLHLFYKIGPNPRDWWGMVITSTDGGRHWSQPTRLPDGILGPIKNKMFVTRDGSWLAPSSREVGTPEKNRWFLKMERSTDRGKTWQAEAEIPSPMNIEAIQPSVLSYPDGRLELISRTRQGALAMSWSKDDGKSWSPLAAIDLPNPNAGTDAVTLQDGRQLIIYNHAAHRPDTPGDGPRWPLNIGVSADGVDWHKALTLESKPMPDGYAYPAVIQTRDGLVHITYTWNRTRIRYVVVDPKKLK
>NZ_CP017578.1|WP_017977670.1|1260293_1261220_+|hypothetical-protein
MTRTLIALALVASPAFAQQQAPFTVVETGQGFTDLQEAVSSIRMGTGTIRIAPGVYRQCAVQQGGRITFRATQPGTAIFETTACEDKAGLVLRGQGSTVDGLVFRGYRVPDGNGAGIRTEMGDLTVTNSMFLDSQEGILGGEPTGQRIRIDHATFSGLGQCDETPDCAHSIYLANRGQVTITNSRFERGRGGHYVKLRVPNVTISDNSFDDTAGQKTNYMIDLPEGARGLITRNTFVQGKAKENHSGLIVVAAEAKTYPSAGLRIEGNDARLAPGAPTNPAFVADLSHERLAVGANRLGPGIRMFETR
>NZ_CP017578.1|WP_017977669.1|1258882_1260091_-|sodium/hydrogen-exchanger
MHAMSATEVFLLAILIIFTLPYAVWRLGRTDYWAPLVVVQIIGGILLGPGILGAIFPDYYAFVFAPATMGALNGIAWWSVMLFVWVAGLELDVGEAWRRRRETGVTAGLALGVPLIAGSLAALVILRYPGWRGPSGANWQVVLGIGMACAVTALPILVLLMEKLAILRAPLGQRVLRYASLDDIAIWGVLALILLDWERVGRQAGFLIGFAVATWAVRKLLRALPENDRWYVGLIWLALCAFAADWAGLHFMVGAFLAGAVLDAHWFGEARMDRFRGTILLAVMPVYFLSTGLKTQWGMGGASVFAVAALLLAASVGGKLAGIHLAGRILKWPKGDASAIGWLLQTKALIMIIFVNILLDKKIITSETFTALLLMAVASTMLTIPVVAPQLRRNPGLAKRGF
>NZ_CP017578.1|WP_017977668.1|1257842_1258697_+|cupin-like-domain-containing-protein
MPGSVALPGFASVAAERDAWRVRTRQRQQALSPVLTAVPRIRGPVSAETFLDLFYAPGIPVVIEGLAAKWPAVRQWTTKKLARKVGSAEITYQGGRNSAPDFEIAKTRHTRTMPFDAYIEMVTSADTGNDAYITAYNNVANTVALQPLMGDFGPVPFLDERIGSIWIGPKGTFTPLHHDLNNNLLVQLMGTKRLFLLPPEETSKLANNDFVFSDVHDIMSDDKVAQYPEVLSARCYHVDIHPGDALFMPVGWWHQVEALSFSAMLTYHNFIWPNDAFRDFPPNR
>NZ_CP017578.1|WP_081609112.1|1254728_1257479_+|methionine-synthase
MESPLHPSASPSGPPPRERGGLEEKTMTNSSAQFVNIGERTNVTGSAAFKKMIMAGDYTRAVEVARQQVENGAQVVDVNMDEGLLDAEHAMTTFLKLIAAEPDIARVPIMIDSSKWSVIEAGLKCVSGKPIVNSISMKEGEESFLHYAKRCMAYGAAVVVMAFDEVGQADTKDRKVEICARAYDLLMGIGFPPEDIIFDPNIFAVATGIEEHNNYGVDFIEACKEIKARCPHVHISGGLSNLSFSFRGNEPVRRAMHSVFLYHAIPAGMDMGIVNAGQLDVYDTIDAELRTACEDVILNRDPEAGDRLVALAERFRGTDAVAEKAAAEWRSLPVVKRLEYALVKGIDAHVVDDTEECRQAFARPIEVIEGPLMDGMNVVGDLFGSGKMFLPQVVKSARVMKKAVAHLLPFIEAAKEPGAKGKGKIVLATVKGDVHDIGKNIVGVVLQCNGFEVVDLGVMVPWSKILEAANENDADMIGLSGLITPSLDEMVTVAEEMKRAGMSMPLLIGGATTSKVHTALRISPAYDGPIVHVLDASRAVGVATTLVSDTQRDAYVAGVAEDYEKVRIARANKGQNDLLPLAEARARGLSVDMALKAPAAAQPGVHVFDDWDLADLRAYIDWTPFFRAWELAGNFPAILTDEVVGESASSLYADAQAMLDRIVAEKWLTARGVAGLWACHRHDDDVWVYTQHREAHRSPDGASIPEGFQIPNLDRTQEASVRLPFLRQQIAKREGRANMCLADFIDPAGDWMGGFAVGIHGIEPHLARFKADNDDYNDILLKALADRLAEAFAERLHAHVRTDLWGYAPGEQLTNEALVREQYRGIRPAPGYPACPEHSLKVTLFEMLDAQGNAGLELTDSFAMLPTAAVSGFYFGHAQAEYFGVARIGEDQVADYATRRGVSVEQATRWLRPNLD
>NZ_CP017578.1|WP_017977666.1|1253604_1254651_+|5-methyltetrahydrofolate--homocysteine-methyltransferase
MTIRDTFLGEAAKRILITDGAFGTEIQNWKLSEADYAGTLGLSHDQKGNNDILALTKPEVPEAIHRAYFEAGADIAETNTFSANRISQADYGAEALVREINVESAKLARRIADEFQAKDGRPRFVAGAIGPTNKTLSLSPDVNDPGYREIDFDYLKDVYREQIDALVEGGADFILIETVFDTLNAKAGIMATLDAAEALGRDLPLMLSMTLTDLSGRNLSGHTVEAFWHAVRHAKPVTIGLNCSFGAEQLRPHVKTLSETCDTLIMVYPNAGLPNELGAYDEAPSTTAGLVGEWASAGQVNVLGGCCGSTPAHIKAIADMARGVAPRTIPTPPVRTRLAGLEPFTMAA
>NZ_CP017578.1|WP_017977665.1|1252489_1253431_+|methylenetetrahydrofolate-reductase-[NAD(P)H]
MTISVNQLEEAQIALKAPLYADVGGHLDVSFEFFPPKTEKMDEQLWEAVRTLEPLGPSFVSVTYGAGGSTRERTHATVARIQRETSLAAAAHLTCVEATRAEIDQVAEDYWAAGVRHIVALRGDPPAAGAKFESHPGGYENAAALVAGLKRLHPFEISVAAYPECHPDSVSVAADLDNLKAKIDAGATRAITQFFHEPETFFRYRDKVAAAGITAEIVPGIMPVTNFASVVRMSKMCGTEVPAWLARLFDGLEDHPAARQLVAATLAAELSRKLYAGGCKSLHFYTLNKAELSFAICHLLGVRAKPMQDAAAA
>NZ_CP017578.1|WP_017977664.1|1251524_1252493_+|metalloregulator-ArsR/SmtB-family-transcription-factor
MAHALDIFRALADATRLRIFSLLRTMELSVGELAQVLGQSQPRVSRHVKILCDAGLAERRKEGSWVFVALGETAATQPVAGAIDAWTQYEPDHWAVADAARLAAVRADRASSAAAWFEGHAGEWDAIRSLHVAESEVEAAMAAVLGTGPLGSLIDIGTGTGRMLELFGDQADSALGIDRSSEMLRLARAKLHDRPNTELRQADLYALPMADGAADVAILHHVLHFAQQPGAAIAEAARVLGPGGRLLIVDFAPHDREELRTRNAHTRLGFADDQIAGWFGNAGLALARTETLGGGELTVTLWLGRKSGASLREVPLQKVGAA
>NZ_CP017578.1|WP_017977663.1|1251046_1251418_-|hypothetical-protein
MKRLLILAAPLALGVAACSQNAQDQTAEAGNAIAADAAATTRNAVSDVDAATDEAFGSAERHLDNAGNAIDRAADRADARADRAGENIDRGLDRAGRSISNAADRAADATGNTLERAGRALKD
>NZ_CP017578.1|WP_017977662.1|1250605_1250845_-|hypothetical-protein
MSNRIATALIPLLLFASLAACSGGSDDPRAVTPDEAAQLNDAAAMLDANSIDLDAVSDDAANDTQPAANPPAANQMDAS
>NZ_CP017578.1|WP_017977672.1|1262621_1264319_-|alkaline-phosphatase-family-protein
MKALIAAALFATAAAPAIAQTAPAPGFRPEPSPAPRIPTSAPAPGATSTALRTPPKLIVAISVDQFSADLFQQYRNHFTEGFARLLTGVVFPSGYQSHAATETCPGHSTILTGMRPAHTGIVANNWVEQNIARADKIVYCTEDETVPGTDHEHYVVSDKHLKVPTLGEMMKAADPRTRVVSVAGKDRAAVMMGGHKVDELWWWDGKTYSSYAGRAVPRAVQRGRDAVAALVAKPQEPLPLPSYCEALDRPITVAGKVYGQGRFARAAGDLKGFRVSPASDAAVFAIAAGLIQDMGLGKGPQTDIIDIGASATDYIGHALGTQGTEMCIQMDQLDHTLGSFLNTLDSWGIDYEVVLTADHGAHDMTERQQQHAMPMEEHVSGDASIKVVNAALMREFGLTVPALVGAEGDVYLGKDLPAALRPRVLAEAQKRFAAMPQVAGAFTRAQIAATPFATTPPENWTLLERARASFNPDVSGDLLVLLKPRVTTIEEPKEGYIETHGSPWDYDRRVPILFWRKGIAGFEQPLSVETVDIAPTLAATIHLPVKGLDGRCLDLDPGTGDTCAR
>NZ_CP017578.1|WP_018250506.1|1264380_1266408_+|hypothetical-protein
MGKPRTAVVAMRALCCALMTIVASLWAVPASADLAPAGGAPHLAMQLVPESDSPRAGQALTIAIATQPEKGWHGYWRNPGDAGFAPALQWTLPKGVTAAPDLQWPVPSTLVVAGLMNYVYEAPYAPLATLRVPAGLAKGTRLPVRLAINYLVCTDTICVPEAQALSLDLTIGDGAPSHGADFDRWRAAIPKPLDAAATYQVTDGKLVMAVPLPAAAKVSDVAFFPYGPDMVTYAAAQSVTRDGDRLLLTTEGAPKGEIAGVLRIGRDLGLSVTAKPGTVASPDDRSGWMAALLAFAGAVLGGLILNVMPCVFPILSLKALHLARGGSDERAARIDALGYMLGTVLMCVGLGALILALRAGGSAVGWAFQLQDPRIVVLLIALTFAITLNLAGRFEVPVPAFASHGGAAGSIATGALAAFVATPCSGPFMGAALGAALVLPWPAALAVFAGLGLGLALPFLAIGFVPALRRRLPKPGLWMERFRRVLAVPMALTVLALLWLAWRQAGTMGLALGLATLVATGVILSLGGTRQRRGQGFGWALALAALVVPAGLAAALTGGAVKPAHTGGDAFSEERLASLRKQGRPVFAYFTADWCLSCKVNEASSIDTNATRAAFERNNVAVLVGDWTDGDPALGRFIQAHNRAGVPLYLYYAPGAAEPRVLPQVLTPGMLAELK
>NZ_CP017578.1|WP_017977674.1|1266666_1268166_+|circularly-permuted-type-2-ATP-grasp-protein
MGKRPAFDEIMGQHGDTPPRPELAALGTWLADTPDDDLRRRQQAAENTFRQLGITFAVYGDTDTRERIIPFDIVPRVFLHDEWARLSEGLVQRVEAINAFLDDIYGERRILKEGVLPADLILGNPQFRPEIAGMRPPHGIWAHICGIDLVRTGPDQFFVLEDNARTPSGVSYMLENREAMVRLCPELFREYRVEPVDSYPDRLLQTMKSVAPPGRGSQPVCVVLTPGHYNSAYYEHSFLADSMGIELVEAADLVVDDDRVWMRTIAGRVQVDVIYRRIDDDFLDPLVFRPDSMLGVPGLIAAYAAGNVAIINAPGNGIADDKAIYSYMPEIVRFYSGGEAKLPNVETWRCREPEALSYVLDHLDEVVVKLVDGSGGYGMLVGPTASKAEIEAFRAALVAEPHRYIAQPTLALSTVPTLTDAGVAPRHVDFRPFVLTGSCGVQVVPGGLTRVALKEGSLVVNSSQGGGTKDSFVLMAEETPRQGQTQTMGGMTQSQGGVA
>NZ_CP017578.1|WP_017977675.1|1268353_1269292_+|alpha-E-domain-containing-protein
MMLSRTGASLYWLGRYLERADFIARLIEATVRLDVMSSRPAGEIAWSSALAVTETEEAFAASGAALRQTEVVRFLTLDTSHPGSVIRCLDRARDNAKAVRTALSREAWTAINRAWLVFENRNQVGDTAQTLSLVEAVKNETRGFEGAVHQMLRNQSHWFIRLGQAVERADNTARLLDVKYHILLPEGEAVGGVVDRDQWTTILQTVSAVTAYRWLYSDGLTPANVIDLLIANRDLPRSLLASAETTVDLLGLLAERTGAHGEADRMARMRAARLGRTRSGEVIASGLHQYLQAFIRENGQLHEAIGRQFRFG
>NZ_CP017578.1|WP_018250505.1|1269291_1270095_+|transglutaminase-family-protein
MRLSIDHRTVYRFSEPQSRLVQMLRMTPENSHDQTVARWRIDVDHDATMRRGRDGFGNAVTMLYVEGPLLDIEIAVRGEVLTSHSDGVVRGVNEVLPPAVFLRATDATPRDPAIAAYAREAVAGADGVIAQLHRLNHAFHGRFALDRRRPEAGLSAAVAFTREAATSRDLAQMFAVAARSLDIPARYVSGYSIVEGSLRPTPHGWAEAHVPGLGWVGFDPCTGRSPQEESVRVAAALDAAGAAPVAGSRLGEGEEELDVAVKVSAAQ
>NZ_CP017578.1|WP_017977677.1|1270450_1271413_+|cell-wall-hydrolase
MLVSAASCVPPRQGVSRVSQAVPPPTPRQLLRDLALDVPGDLPGLAGLPASLRDAVDGDDAAAVAPQAAPPFLAQMHSQEDAARATDCLTAAVYYEARSEPIDGQRAVAQVVLNRVRDRAFPHSVCGVVFQGSTRRTGCQFSFTCDGSMAHRREPYAWARARSVAEAALAGNVYAPVGGATFYHTDAILPWWASSLTRIGSVGAHIFYRWRGAMESALSFRAAYAGVEPGVVAGGGDGAAQPQVATLYRDSQDDRGVTVHRGRMTPVASVAEAPTTDAPKADAPAPQMMIAAGVRVHRGSEAPRRLMTEGAVIGAESDPT
>NZ_CP017578.1|WP_024310446.1|1271419_1272211_-|arginyltransferase
MTAPFRFPRFFVTTPAPCPYLPGRQERKVFTELSGPHAAELNDALGRIGFRRSQGVAYRPSCAGCAACVSVRVVTDEFVPNATQRKLLRRNEDLEVSACRPWATDEQFQLLRRYLGARHPGGGMAGMDEEDYADMVEHSPVNSHVIEYRTPSVGGVRGQLVGACLTDRQADGLSMIYSFFDADGDDPATARPGLGNYIIMDHILRARDSGLPYVYLGYWVKGSARMAYKTRYRPIEMLGPNGWRALTDEELAPPPLPDAMAVA
>NZ_CP017578.1|WP_017977679.1|1272465_1272714_+|hypothetical-protein
MTKIAGSLAALALTTASIATPAFAAPVNPAASLSVAKSVRASAPSAQKNKLAGGGLLAAIIAASVVAIGVVAIVDDGNSDSN
>NZ_CP017578.1|WP_018250504.1|1272826_1273324_-|hypothetical-protein
MYAARAKRTYPSIWRVILAFVVVPGAAALLMAIAMPAYEGITDPLERIWRSAVAFAVFGAYPPAFIIGLPAFFMLRRHVNATIINCAATGAVVAALPWLVLALISRPDNASIDGRSTVIDGSLTAYGWLMNFYYVGQIALLGAIAGALFWFIAAAGSRTGKVEQI
>NZ_CP017578.1|WP_017979475.1|1273646_1274810_-|IS110-family-transposase
MPVSVSCHAAVSTQLNAIFVSLELSRSTWLVTSLSPGRGERISRHQVKGSDVPALLERFAQLQAKAEARMGEQYPLVVIQEAGLDGFWIHRCLVREGIESYVVDAASIAASRRSRRAKTDKLDGEALVRTLLAYKRGEPRVCAMVAVPSVEDEDRRRISRERRALVVERVRHVNRIKGLLFAQGVGDYEPLRADRRARLDELVTGDGRPLPRHVRREIERELDRLEMVVGQIKQVEAERDALLEPRSDAAGEADTGPRLIQLRGIGPEFAGVLHAEAFFRSFSNRRQVAAYAGLAPSPWQSGSMDREQGVSKAGNKRLRTTMIQVAWLWLRHQPDSELTKWYAERVGANGGRARRVMIVAVARKLLIALWQFVSAGVVPAGALLKAA

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Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity
NZ_CP017578_1	1.1\|1262455\|52\|NZ_CP017578\|CRISPRCasFinder	1262455-1262506	52	NZ_CP009572	Sphingomonas taxi strain ATCC 55669 plasmid STP1, complete sequence	160147-160198	11	0.788

1. spacer 1.1|1262455|52|NZ_CP017578|CRISPRCasFinder matches to NZ_CP009572 (Sphingomonas taxi strain ATCC 55669 plasmid STP1, complete sequence) position: , mismatch: 11, identity: 0.788

ctgcccctgcggctgggtggaccccggaacaagtccggggtgacgaggggtg	CRISPR spacer
gcacgcctgcggctcggtggaccccggaacgagtccggggtgacggatattg	Protospacer
 ..* ********* ***************.**************.. . **

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

94196 : 108693

Rhizobium_phage(30.0%)

terminase,capsid,protease,portal,tail,head

The bacterium proteins that are colored denote the protein is present at specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin' and 'tRNA')

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_062125875.1\|94196_95318_+	DNA polymerase III subunit beta	B4UTW9	Rhizobium_phage	6.4e-46	37.8
WP_017978568.1\|95329_95527_+	hypothetical protein	NA	NA	NA	NA
WP_062125871.1\|95529_96165_+	hypothetical protein	NA	NA	NA	NA
WP_082824938.1\|96314_96638_+	DUF2312 domain-containing protein	J7FAQ3	Agrobacterium_phage	1.6e-13	58.1
WP_062125868.1\|96637_97099_+	single-stranded DNA-binding protein	R9TP78	Rhizobium_phage	7.9e-43	55.0
WP_062125866.1\|97185_100047_+	DNA primase	R9TSC7	Rhizobium_phage	8.9e-76	32.7
WP_062125863.1\|100335_100818_+	hypothetical protein	NA	NA	NA	NA
WP_062125924.1\|100981_101335_+	HNH endonuclease	I3ULZ4	Rhodobacter_phage	1.5e-33	61.7
WP_062125860.1\|101331_101547_+	hypothetical protein	NA	NA	NA	NA
WP_062125858.1\|101689_102169_+	hypothetical protein	NA	NA	NA	NA
WP_062125856.1\|102170_103928_+\|terminase	terminase large subunit	Q3HQS7	Burkholderia_phage	3.3e-150	49.8
WP_082824937.1\|103981_105214_+\|portal	phage portal protein	A0A0U2BXP2	Paracoccus_phage	2.1e-106	49.9
WP_156487358.1\|105210_105510_+	hypothetical protein	NA	NA	NA	NA
WP_062125854.1\|105509_106139_+\|head,protease	HK97 family phage prohead protease	A0A2H4JAI0	uncultured_Caudovirales_phage	1.0e-37	47.7
WP_082824935.1\|106162_107458_+\|capsid	phage major capsid protein	A0A2H4JD98	uncultured_Caudovirales_phage	6.4e-98	50.3
WP_062125852.1\|107535_108027_+	hypothetical protein	NA	NA	NA	NA
WP_070240660.1\|108034_108349_+\|head,tail	phage gp6-like head-tail connector protein	NA	NA	NA	NA
WP_062125847.1\|108348_108693_+\|head,tail	head-tail adaptor protein	A0A0U2BXJ0	Paracoccus_phage	3.1e-12	43.9

DBSCAN-SWA_2

2901917 : 2968750

Escherichia_phage(22.22%)

tRNA,transposase

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_017979895.1\|2901917_2903030_+\|tRNA	tRNA 2-thiouridine(34) synthase MnmA	NA	NA	NA	NA
WP_018250646.1\|2903026_2903281_+	hypothetical protein	NA	NA	NA	NA
WP_017979897.1\|2903419_2904217_-	PhzF family phenazine biosynthesis protein	NA	NA	NA	NA
WP_017979898.1\|2904216_2905170_-	cation diffusion facilitator family transporter	NA	NA	NA	NA
WP_017979899.1\|2905166_2905745_-	pyridoxamine 5'-phosphate oxidase	NA	NA	NA	NA
WP_017979900.1\|2905866_2906742_+	Ku protein	NA	NA	NA	NA
WP_070240687.1\|2912903_2914064_-	hypothetical protein	NA	NA	NA	NA
WP_167692823.1\|2914070_2915687_-	glycosyltransferase	NA	NA	NA	NA
WP_062126906.1\|2915731_2916475_-	hypothetical protein	NA	NA	NA	NA
WP_156487330.1\|2916556_2917750_-	hypothetical protein	NA	NA	NA	NA
WP_156487331.1\|2917746_2919690_-	hypothetical protein	NA	NA	NA	NA
WP_063113184.1\|2919782_2921837_-	asparagine synthase (glutamine-hydrolyzing)	NA	NA	NA	NA
WP_062126931.1\|2921840_2923988_-	Gfo/Idh/MocA family oxidoreductase	NA	NA	NA	NA
WP_156487332.1\|2924286_2925585_+	polyprenyl glycosylphosphotransferase	NA	NA	NA	NA
WP_062127390.1\|2926351_2927551_+\|transposase	IS256 family transposase	A0A218MNI5	uncultured_virus	1.6e-42	42.8
WP_062127305.1\|2930635_2930914_-	hypothetical protein	NA	NA	NA	NA
WP_070240689.1\|2930903_2931572_-	ParA family protein	B0ZSI1	Halomonas_phage	7.0e-08	25.1
WP_062127311.1\|2931822_2932635_-	helix-turn-helix domain-containing protein	NA	NA	NA	NA
WP_156487348.1\|2934468_2934645_+	hypothetical protein	NA	NA	NA	NA
WP_156487365.1\|2934901_2936001_-\|transposase	IS3 family transposase	S5WIU1	Leptospira_phage	7.7e-52	43.4
WP_082824919.1\|2936340_2937756_+	L-serine ammonia-lyase	NA	NA	NA	NA
WP_062127033.1\|2937840_2938896_+	dTDP-glucose 4,6-dehydratase	A0A1D7XFE8	Escherichia_phage	3.0e-85	46.9
WP_062127036.1\|2938892_2939819_+	glucose-1-phosphate thymidylyltransferase RfbA	I7I009	Enterobacteria_phage	6.2e-87	54.9
WP_062127037.1\|2940188_2941208_-	phytase	NA	NA	NA	NA
WP_062127070.1\|2941204_2943880_-	TonB-dependent receptor	NA	NA	NA	NA
WP_070240691.1\|2944126_2944891_-	tyrosine-protein phosphatase	NA	NA	NA	NA
WP_062127045.1\|2945075_2947445_-	TonB-dependent receptor	NA	NA	NA	NA
WP_062127048.1\|2947624_2948791_+	PQQ-dependent sugar dehydrogenase	NA	NA	NA	NA
WP_167692824.1\|2948974_2949685_-	RES domain-containing protein	NA	NA	NA	NA
WP_062127055.1\|2950314_2953680_+	S8 family serine peptidase	NA	NA	NA	NA
WP_062127058.1\|2953880_2954312_+	DUF2384 domain-containing protein	NA	NA	NA	NA
WP_062127061.1\|2954308_2954746_+	RES family NAD+ phosphorylase	NA	NA	NA	NA
WP_062127064.1\|2954817_2955261_+	hypothetical protein	NA	NA	NA	NA
WP_156782885.1\|2955290_2955989_-	hypothetical protein	NA	NA	NA	NA
WP_037447094.1\|2957950_2958448_-	hypothetical protein	NA	NA	NA	NA
WP_062127316.1\|2958482_2958785_-	hypothetical protein	NA	NA	NA	NA
WP_062127319.1\|2958989_2959586_+	recombinase family protein	E5FFF9	Burkholderia_phage	9.0e-39	50.3
WP_062127322.1\|2959578_2961231_+\|transposase	IS481 family transposase	NA	NA	NA	NA
WP_037447103.1\|2961595_2963155_-\|transposase	IS66 family transposase	A0A0P0ZBS5	Stx2-converting_phage	9.6e-117	45.7
WP_119184635.1\|2963210_2963504_-	IS66 family insertion sequence element accessory protein TnpB	A0A0P0ZDM8	Stx2-converting_phage	3.9e-27	62.0
WP_007407193.1\|2963554_2963917_-	IS66 family insertion sequence hypothetical protein	NA	NA	NA	NA
WP_061781607.1\|2964651_2965380_-	ATP-binding protein	A0A2L1IVB6	Escherichia_phage	1.7e-31	33.2
WP_083273243.1\|2965366_2966878_-\|transposase	IS21 family transposase	NA	NA	NA	NA
WP_083273268.1\|2966953_2967226_+	hypothetical protein	NA	NA	NA	NA
WP_062127199.1\|2967394_2968750_+\|transposase	transposase	NA	NA	NA	NA

DBSCAN-SWA_3

2980007 : 3034118

Stx2-converting_phage(16.67%)

integrase,transposase

attL 3027373:3027389|attR 3037441:3037457

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_056439051.1\|2980007_2980385_+\|transposase	transposase	NA	NA	NA	NA
WP_076711207.1\|2980381_2980729_+	IS66 family insertion sequence element accessory protein TnpB	A0A0P0ZDM8	Stx2-converting_phage	4.7e-32	60.7
WP_070240769.1\|2980840_2982274_+\|transposase	IS66 family transposase	A0A0P0ZEB3	Stx2-converting_phage	1.8e-130	52.3
WP_070240697.1\|2982258_2983356_-	hypothetical protein	NA	NA	NA	NA
WP_062127172.1\|2983437_2986800_-	relaxase domain-containing protein	NA	NA	NA	NA
WP_156487343.1\|2986796_2987594_-	hypothetical protein	NA	NA	NA	NA
WP_062127165.1\|2987709_2988135_+	helix-turn-helix domain-containing protein	NA	NA	NA	NA
WP_062127162.1\|2988137_2988404_+	hypothetical protein	NA	NA	NA	NA
WP_082824924.1\|2989355_2989988_-	recombinase family protein	A0A0C4UR34	Shigella_phage	2.6e-36	49.2
WP_062127154.1\|2990125_2993095_+\|transposase	Tn3 family transposase	A0A1B0V7H9	Salmonella_phage	3.2e-230	44.3
WP_062127151.1\|2993501_2994713_+\|transposase	IS256 family transposase	A0A220NQR7	Corynebacterium_phage	2.7e-98	48.8
WP_082824923.1\|2994741_2995119_-\|transposase	transposase	NA	NA	NA	NA
WP_082824922.1\|2995137_2995356_-	hypothetical protein	NA	NA	NA	NA
WP_062127390.1\|2995728_2996928_+\|transposase	IS256 family transposase	A0A218MNI5	uncultured_virus	1.6e-42	42.8
WP_156487328.1\|2997792_2998167_-	hypothetical protein	NA	NA	NA	NA
WP_156487329.1\|2998177_2999338_-	hypothetical protein	NA	NA	NA	NA
WP_062126865.1\|2999423_3001637_-	polysaccharide biosynthesis tyrosine autokinase	NA	NA	NA	NA
WP_062126868.1\|3001653_3002367_-	polysaccharide export protein	NA	NA	NA	NA
WP_063113181.1\|3002406_3003786_-	O-antigen ligase family protein	NA	NA	NA	NA
WP_070240699.1\|3003829_3004780_-	hypothetical protein	NA	NA	NA	NA
WP_083273245.1\|3004857_3005472_-	hypothetical protein	NA	NA	NA	NA
WP_062126885.1\|3005353_3006616_-	UDP-N-acetyl-D-mannosamine dehydrogenase	M1HZW8	Acanthocystis_turfacea_Chlorella_virus	1.1e-22	26.4
WP_062126889.1\|3006612_3007761_-	UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing)	A0A1V0SAG5	Catovirus	6.8e-27	28.6
WP_062126937.1\|3007757_3008984_-	glycosyltransferase family 4 protein	NA	NA	NA	NA
WP_083273246.1\|3009035_3010631_-	alginate lyase family protein	NA	NA	NA	NA
WP_062126896.1\|3010627_3012742_-	Gfo/Idh/MocA family oxidoreductase	NA	NA	NA	NA
WP_062126899.1\|3012738_3014064_-	hypothetical protein	NA	NA	NA	NA
WP_167692825.1\|3014070_3015231_-	glycosyltransferase	NA	NA	NA	NA
WP_070240702.1\|3015125_3015725_-	hypothetical protein	NA	NA	NA	NA
WP_062126906.1\|3015730_3016474_-	hypothetical protein	NA	NA	NA	NA
WP_156487330.1\|3016555_3017749_-	hypothetical protein	NA	NA	NA	NA
WP_070240704.1\|3018598_3019582_-	hypothetical protein	NA	NA	NA	NA
WP_167692826.1\|3019724_3020048_-	hypothetical protein	NA	NA	NA	NA
WP_083273250.1\|3020026_3020377_-	asparagine synthase	NA	NA	NA	NA
WP_156782886.1\|3022127_3022289_+	hypothetical protein	NA	NA	NA	NA
WP_156782887.1\|3022358_3022643_-	hypothetical protein	NA	NA	NA	NA
WP_156782888.1\|3023296_3024475_-	serine hydrolase	NA	NA	NA	NA
WP_062126245.1\|3024728_3025733_+	UDP-glucose 4-epimerase GalE	A0A2K9L5H6	Tupanvirus	9.5e-41	34.8
WP_062126248.1\|3025769_3026726_+	SDR family oxidoreductase	A0A2K9KZK0	Tupanvirus	1.1e-83	46.6
WP_062126252.1\|3027214_3028369_+	hypothetical protein	NA	NA	NA	NA
3027373:3027389	attL	NA	NA	NA	NA
WP_062126255.1\|3028382_3029855_+	D-glycero-beta-D-manno-heptose-7-phosphate kinase	A0A0K0KVL9	Prochlorococcus_phage	9.7e-18	40.3
WP_062126258.1\|3029851_3030841_+	ADP-glyceromanno-heptose 6-epimerase	A0A1D8KJQ4	Synechococcus_phage	2.2e-26	30.3
WP_082824957.1\|3030827_3031478_+	HAD-IIIA family hydrolase	NA	NA	NA	NA
WP_156487384.1\|3031586_3032402_-	SAM-dependent methyltransferase	NA	NA	NA	NA
WP_062126265.1\|3033041_3034118_-\|integrase	tyrosine-type recombinase/integrase	NA	NA	NA	NA
3037441:3037457	attR	NA	NA	NA	NA

DBSCAN-SWA_4

3063449 : 3072405

Stx2-converting_phage(66.67%)

transposase

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_062127357.1\|3063449_3064616_-	protein kinase	A0A0M3SGV1	Mollivirus	1.2e-07	30.7
WP_156487352.1\|3065808_3066021_-	hypothetical protein	NA	NA	NA	NA
WP_062127372.1\|3066235_3066598_+	IS66 family insertion sequence hypothetical protein	NA	NA	NA	NA
WP_119184635.1\|3066648_3066942_+	IS66 family insertion sequence element accessory protein TnpB	A0A0P0ZDM8	Stx2-converting_phage	3.9e-27	62.0
WP_070240668.1\|3066997_3068557_+\|transposase	IS66 family transposase	A0A0P0ZBS5	Stx2-converting_phage	1.7e-121	47.2
WP_062127372.1\|3068693_3069056_+	IS66 family insertion sequence hypothetical protein	NA	NA	NA	NA
WP_119184635.1\|3069106_3069400_+	IS66 family insertion sequence element accessory protein TnpB	A0A0P0ZDM8	Stx2-converting_phage	3.9e-27	62.0
WP_070240668.1\|3069455_3071015_+\|transposase	IS66 family transposase	A0A0P0ZBS5	Stx2-converting_phage	1.7e-121	47.2
WP_062127343.1\|3071187_3072405_-	hypothetical protein	Q858S3	Enterobacteria_phage	1.7e-20	46.9

DBSCAN-SWA_5

3913472 : 3920977

Caulobacter_virus(16.67%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_017978379.1\|3913472_3914234_-	serine/threonine protein phosphatase	K4JTY8	Caulobacter_virus	1.8e-23	35.1
WP_024310508.1\|3914319_3915618_-	UDP-N-acetyl-D-mannosamine dehydrogenase	M1HFY9	Paramecium_bursaria_Chlorella_virus	4.0e-23	28.6
WP_017978381.1\|3915601_3916711_-	UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing)	NA	NA	NA	NA
WP_017978382.1\|3916825_3917695_+	UTP--glucose-1-phosphate uridylyltransferase GalU	A0A127AW70	Bacillus_phage	8.1e-65	48.9
WP_017978383.1\|3917699_3918452_-	molybdopterin-synthase adenylyltransferase MoeB	A0A1V0SAV8	Catovirus	2.5e-09	28.9
WP_017978384.1\|3918474_3918870_-	hypothetical protein	NA	NA	NA	NA
WP_017978385.1\|3918866_3919310_-	dUTP diphosphatase	K4JSC8	Caulobacter_phage	6.9e-36	59.3
WP_017978386.1\|3919358_3919742_-	VOC family protein	NA	NA	NA	NA
WP_017978387.1\|3919795_3920977_-	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	Q9HH70	Methanothermobacter_phage	1.0e-38	33.1

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage