CRISPRimmunity

Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Self targeting spacer number	Target MGE spacer number	Prophage number	Anti-CRISPR protein number
NZ_CP019572	Rhodococcus sp. MTM3W5.2 chromosome, complete genome	2 crisprs	WYL,cas3,csa3,cas4,DEDDh	0	1	0	0

1. NZ_CP019572

CRISPR-Cas detection and classification

Crispr_ID: NZ_CP019572_1

• CRISPR_ID: NZ_CP019572_1
• CRISPR_location: 3115392-3115493
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 1 spacers

Consensus repeat of NZ_CP019572_1

Consensus_repeat	Method
CGAACGCGGTGCAGCCCTTGCGGAGACGGCACAGGA	CRISPRCasFinder

spacers of NZ_CP019572_1

>1.1|3115428|30|NZ_CP019572|CRISPRCasFinder
ACACGCCCCGGAACTCCTCGCGCTCGCCCG

CRISPR arrays and Neighbor proteins around NZ_CP019572_1

CRISPR arrays

The CRISPR arrays of NZ_CP019572_1

>merge|NZ_CP019572|1|3115392-3115493|CRISPRCasFinder
CGAACGCGGTGCAGCCCTTGCGGAGACGGCACAGGAACACGCCCCGGAACTCCTCGCGCTCGCCCGCGAACGCGGTGCAGCCCTTGCGGAGACGGCACAGGA

>NZ_CP019572|1|1|3115392-3115493|CRISPRCasFinder
CGAACGCGGTGCAGCCCTTGCGGAGACGGCACAGGA	ACACGCCCCGGAACTCCTCGCGCTCGCCCG
CGAACGCGGTGCAGCCCTTGCGGAGACGGCACAGGA

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
NZ_CP019572.1|WP_027503190.1|3108368_3108563_-|50S-ribosomal-protein-L35	unknown	unknown	gnl|CDD|234676
NZ_CP019572.1|WP_077041811.1|3118548_3119001_-|universal-stress-protein	unknown	unknown	gnl|CDD|366180
NZ_CP019572.1|WP_077041810.1|3116178_3118479_+|AAA-family-ATPase	unknown	unknown	gnl|CDD|226482
NZ_CP019572.1|WP_077041801.1|3102804_3103278_-|hypothetical-protein	unknown	unknown	gnl|CDD|224755
NZ_CP019572.1|WP_077041802.1|3103390_3105877_-|phenylalanine--tRNA-ligase-subunit-beta	unknown	unknown	gnl|CDD|234804
NZ_CP019572.1|WP_077041806.1|3110003_3110456_+|DUF1801-domain-containing-protein	unknown	unknown	gnl|CDD|378058
NZ_CP019572.1|WP_027502000.1|3127961_3128174_+|hypothetical-protein	unknown	unknown	unknown
NZ_CP019572.1|WP_077041807.1|3114080_3114770_+|MBL-fold-metallo-hydrolase	unknown	unknown	gnl|CDD|293792
NZ_CP019572.1|WP_077041817.1|3126616_3127837_-|dephospho-CoA-kinase	unknown	unknown	gnl|CDD|179560
NZ_CP019572.1|WP_077041813.1|3121959_3122871_+|ABC-transporter-permease	unknown	unknown	gnl|CDD|224196
NZ_CP019572.1|WP_077041803.1|3105940_3107011_-|phenylalanine--tRNA-ligase-subunit-alpha	unknown	unknown	gnl|CDD|234780
NZ_CP019572.1|WP_083742936.1|3115670_3116060_+|hypothetical-protein	unknown	unknown	gnl|CDD|226867
NZ_CP019572.1|WP_077041815.1|3123824_3125987_-|excinuclease-ABC-subunit-B	unknown	unknown	gnl|CDD|235395
NZ_CP019572.1|WP_077041812.1|3119149_3121963_+|alpha/beta-fold-hydrolase	unknown	unknown	gnl|CDD|224054
NZ_CP019572.1|WP_077043710.1|3108616_3109324_-|translation-initiation-factor-IF-3	unknown	unknown	gnl|CDD|234582
NZ_CP019572.1|WP_027503191.1|3107911_3108301_-|50S-ribosomal-protein-L20	unknown	unknown	gnl|CDD|179959
NZ_CP019572.1|WP_077041816.1|3126067_3126577_+|DUF402-domain-containing-protein	unknown	unknown	gnl|CDD|225188
NZ_CP019572.1|WP_077041814.1|3122887_3123754_-|hypothetical-protein	unknown	unknown	unknown
NZ_CP019572.1|WP_077041805.1|3109617_3109962_+|recombinase-RecA	unknown	unknown	unknown
NZ_CP019572.1|WP_077041804.1|3107075_3107888_-|RNA-methyltransferase	unknown	unknown	gnl|CDD|223640

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
NZ_CP019572.1|WP_027503190.1|3108368_3108563_-|50S-ribosomal-protein-L35	gnl|CDD|234676	PRK00172, rpmI, 50S ribosomal protein L35; Reviewed.	3.72494e-22
NZ_CP019572.1|WP_077041811.1|3118548_3119001_-|universal-stress-protein	gnl|CDD|366180	pfam00582, Usp, Universal stress protein family. The universal stress protein UspA is a small cytoplasmic bacterial protein whose expression is enhanced when the cell is exposed to stress agents. UspA enhances the rate of cell survival during prolonged exposure to such conditions, and may provide a general "stress endurance" activity. The crystal structure of Haemophilus influenzae UspA reveals an alpha/beta fold similar to that of the Methanococcus jannaschii MJ0577 protein, which binds ATP, though UspA lacks ATP-binding activity.	1.08365e-23
NZ_CP019572.1|WP_077041810.1|3116178_3118479_+|AAA-family-ATPase	gnl|CDD|226482	COG3973, COG3973, Superfamily I DNA and RNA helicases [General function prediction only].	7.0878e-122
NZ_CP019572.1|WP_077041801.1|3102804_3103278_-|hypothetical-protein	gnl|CDD|224755	COG1842, PspA, Phage shock protein A (IM30), suppresses sigma54-dependent transcription [Transcription / Signal transduction mechanisms].	5.83267e-05
NZ_CP019572.1|WP_077041802.1|3103390_3105877_-|phenylalanine--tRNA-ligase-subunit-beta	gnl|CDD|234804	PRK00629, pheT, phenylalanyl-tRNA synthetase subunit beta; Reviewed.	0
NZ_CP019572.1|WP_077041807.1|3114080_3114770_+|MBL-fold-metallo-hydrolase	gnl|CDD|293792	cd06262, metallo-hydrolase-like_MBL-fold, mainly hydrolytic enzymes and related proteins which carry out various biological functions; MBL-fold metallohydrolase domain. Members of the MBL-fold metallohydrolase superfamily are mainly hydrolytic enzymes which carry out a variety of biological functions. The class B metal beta-lactamases (MBLs) for which this fold was named perform only a small fraction of the activities included in this superfamily. Activities carried out by superfamily members include class B beta-lactamases which can catalyze the hydrolysis of a wide range of beta-lactam antibiotics, hydroxyacylglutathione hydrolases (also called glyoxalase II) which hydrolyze S-d-lactoylglutathione to d-lactate in the second step of the glycoxlase system, AHL lactonases which catalyze the hydrolysis and opening of the homoserine lactone rings of acyl homoserine lactones (AHLs), persulfide dioxygenase which catalyze the oxidation of glutathione persulfide to glutathione and persulfite in the mitochondria, flavodiiron proteins which catalyze the reduction of oxygen and/or nitric oxide to water or nitrous oxide respectively, cleavage and polyadenylation specificity factors such as the Int9 and Int11 subunits of Integrator, Sdsa1-like and AtsA-like arylsulfatases, 5'-exonucleases human SNM1A and yeast Pso2p, ribonuclease J which has both 5'-3' exoribonucleolytic and endonucleolytic activity and ribonuclease Z which catalyzes the endonucleolytic removal of the 3' extension of the majority of tRNA precursors, cyclic nucleotide phosphodiesterases which decompose cyclic adenosine and guanosine 3', 5'-monophosphate (cAMP and cGMP) respectively, insecticide hydrolases, and proteins required for natural transformation competence. The diversity of biological roles is reflected in variations in the active site metallo-chemistry, for example classical members of the superfamily are di-, or less commonly mono-, zinc-ion-dependent hydrolases, human persulfide dioxygenase ETHE1 is a mono-iron binding member of the superfamily; Arabidopsis thaliana hydroxyacylglutathione hydrolases incorporates iron, manganese, and zinc in its dinuclear metal binding site, and flavodiiron proteins contains a diiron site.	4.25164e-43
NZ_CP019572.1|WP_077041817.1|3126616_3127837_-|dephospho-CoA-kinase	gnl|CDD|179560	PRK03333, coaE, dephospho-CoA kinase/protein folding accessory domain-containing protein; Provisional.	0
NZ_CP019572.1|WP_077041813.1|3121959_3122871_+|ABC-transporter-permease	gnl|CDD|224196	COG1277, NosY, ABC-type transport system involved in multi-copper enzyme maturation, permease component [General function prediction only].	2.50005e-07
NZ_CP019572.1|WP_077041803.1|3105940_3107011_-|phenylalanine--tRNA-ligase-subunit-alpha	gnl|CDD|234780	PRK00488, pheS, phenylalanyl-tRNA synthetase subunit alpha; Validated.	0
NZ_CP019572.1|WP_083742936.1|3115670_3116060_+|hypothetical-protein	gnl|CDD|226867	COG4460, COG4460, Uncharacterized protein conserved in bacteria [Function unknown].	1.87063e-09
NZ_CP019572.1|WP_077041815.1|3123824_3125987_-|excinuclease-ABC-subunit-B	gnl|CDD|235395	PRK05298, PRK05298, excinuclease ABC subunit UvrB.	0
NZ_CP019572.1|WP_077041812.1|3119149_3121963_+|alpha/beta-fold-hydrolase	gnl|CDD|224054	COG1131, CcmA, ABC-type multidrug transport system, ATPase component [Defense mechanisms].	2.59385e-87
NZ_CP019572.1|WP_077043710.1|3108616_3109324_-|translation-initiation-factor-IF-3	gnl|CDD|234582	PRK00028, infC, translation initiation factor IF-3; Reviewed.	3.50519e-97
NZ_CP019572.1|WP_027503191.1|3107911_3108301_-|50S-ribosomal-protein-L20	gnl|CDD|179959	PRK05185, rplT, 50S ribosomal protein L20; Provisional.	7.75884e-59
NZ_CP019572.1|WP_077041816.1|3126067_3126577_+|DUF402-domain-containing-protein	gnl|CDD|225188	COG2306, COG2306, Predicted RNA-binding protein, associated with RNAses E/G family [General function prediction only].	1.00591e-35
NZ_CP019572.1|WP_077041806.1|3110003_3110456_+|DUF1801-domain-containing-protein	gnl|CDD|378058	pfam08818, DUF1801, Domain of unknown function (DU1801). This large family of bacterial proteins is uncharacterized. They contain a presumed domain about 110 amino acids in length.	2.77541e-18
NZ_CP019572.1|WP_077041804.1|3107075_3107888_-|RNA-methyltransferase	gnl|CDD|223640	COG0566, SpoU, rRNA methylases [Translation, ribosomal structure and biogenesis].	1.18529e-64

Protein sequences

>NZ_CP019572.1|WP_077041807.1|3114080_3114770_+|MBL-fold-metallo-hydrolase
MDGQQMTIDDHYTGELTADSDPQRRSFAGASITKISVGPMDNNAYLVVCSETGESLLIDAANDADRLVSLVGAAAPTLTSIVTTHQHPDHWLALAEVVGKTGVPTIAHALDAGPLPVAPDRTVTDGDLVEVGRLSLEVIHLAGHTPGSIALALTDPATGQVHLFSGDSLFPGGVGKTADAEKFASLYRDVTTKLFERFPDDTVVYPGHGKDTTIGAERPFLADWQERGW
>NZ_CP019572.1|WP_077041806.1|3110003_3110456_+|DUF1801-domain-containing-protein
MAAGVSGDTDPVANKTQPTGADVDEFLGTLTDDVKRADCERLIDLMRAATGEPAAMWGSSIIGFGDRTYRYASGHTGNTALIGFAPRSTALTLYLSLNLDDHRDALQRLGKHRTGKGCLYIKRLADVDESELRALIESSVRAARALSADS
>NZ_CP019572.1|WP_077041805.1|3109617_3109962_+|recombinase-RecA
MTEQTHTGDVRELADVPAVEVISRAAVMLMSSAAEKLGLSSPDPDESDHLDLDEARRVITALAGLVTASVEYLGPHAGPIREGLQALQRAFREASAHPDEPGNGPGEKYTGPVY
>NZ_CP019572.1|WP_077043710.1|3108616_3109324_-|translation-initiation-factor-IF-3
MPAVRTTTPLNLGGPISTETRINDRIRVPEVRLVGPGGEQVGIVRVEDALRLALEADLDLVEVAPDARPPVCKIMDYGKFKYEAAQKARESRKNQTLTVIKEQKLRPKIDDHDYETKKRNVVRFLEAGSKVKVTIMFRGREQSRPELGFRLLQRLGADVADLGFVETSAKQDGRNMTMVLAPHKGAKTRVKAQESAQAPVPQVPRPASAPPVPEAPAAAAPEAPAEGAAPEAPTS
>NZ_CP019572.1|WP_027503190.1|3108368_3108563_-|50S-ribosomal-protein-L35
MPKMKSHSGTSKRFKVSGSGKILRQKAGRRHLLEHKPTKVTRRLDGKAVVSKADTPRIKRLLGI
>NZ_CP019572.1|WP_027503191.1|3107911_3108301_-|50S-ribosomal-protein-L20
MARVKRAVNAQKKRRSILEASKGYRGQRSRLYTKAKEQQLHSLTYAYRDRRARKGDFRKLWITRINAAARANDMTYNRFIQGLNAAGVEVDRKILAELAVSDAEAFAGLVAIAKAALPADVNAPAGEAA
>NZ_CP019572.1|WP_077041804.1|3107075_3107888_-|RNA-methyltransferase
MDPFTERTPRVVSAVKLLRGAERKKAGRFLAEGANSVTAALATGRVEELFFTDEAAGRYGDLIAGATAAGVRVSPITERAARSLSDTVTPPGLVAVCGLLDVPLGEALDGSPRLLAVPVSVSEPGNAGTVIRVADAVGADAAILAGDSVDPHNGKCVRSSAGSVFHLPIARERDVALVLEQITAAGVQILATAADGEVDLDDADELLALPTAWLFGNEAHGLDPVIAARADHRVRIPIHGRAESLNLATAAAICLYASARVQHRQDRPTT
>NZ_CP019572.1|WP_077041803.1|3105940_3107011_-|phenylalanine--tRNA-ligase-subunit-alpha
MAKNEAESSGPIDTEALTEDALGAAAERAEAAFAAAGDLDALAKAKIDHLGDKAPLSLVRRALGALPGKEKADAGKRVNAARTRATTAFDARREVLLAERDAAVLVAEAIDVTLPAQRRPVGARHPITIISEQVSDVFVAMGWEVAEGPEVETEHFNFDALNFLPDHPARTMQDTFHIAPEGSRQVLRTHTSPVQVRTMLSRDVPIYVVCPGRTFRTDELDATHTPVFSQLEGLAIDKGLTMAHLRGTLDAFARALFGPDTRTRMRPNYFPFTEPSAEVDVWFPGKKGGAGWVEWGGCGMVNPKVLRASGIDPDEYSGFAFGMGLERTLQFRNGIPDMRDIVEGDVRFTLPFGVRA
>NZ_CP019572.1|WP_077041802.1|3103390_3105877_-|phenylalanine--tRNA-ligase-subunit-beta
MRVAQSWLTEILQRATPDWEVTAEELDAGFVRVGLEVEEVDVLHRVPGPLVVGRVVSITELTEFKKPIRMCQVDVGESEPRGIVCGARNFAEGDLIVAALPGAVLPGDFKIAARKTYGQVSDGMICSVAELGIGKDHSGILVLEPGTAEPGADANDLLGLGDTIIELNITPDRGYCFSVRGLTRELACGFDLEFADPAVLPTHGADGEAWPLTVLPESGSPRFALRRVTGIDPAAVSPWWLQRRLLLSGVRPISPAVDVTNYVLLELGQPLHAFDANKLTGGLVVRRAAADEKLTTLDGVERTLDAEDVVIADETGAVSLAGVMGGASTEVGAGTTEVLLEAAIWNPLAVFRTSRRHKLISEASKRYEREVDQALPVAALDRAAALLVEIAGGRIEPVLTDVGGVNEPAPIRIDIDLPDRVAGVAYPNGTAARRLTQVGCAVDVGVSEAGHGQLVVTPPTWRPDLEQAADLVEEVLRLEGLEQIPSVLPAAPAGRGLTPQQRRRRAVGRALAFDGYVEVLPTVFLPTGVFDTWGLDADDPRRTTSKVLNPLEADRPELASTLLPGLLEILSRNTSRGQRDLALFGIAQVVLPGPDTKPVQALPVDRRPTDDQIADLLRSLPAQPTHVAAVLTGLRVPSGPWGSGRAVEASDAFAAAETIAEAAGVSLERRAAQYLPWHPGRGAELLVDGIVVGHAGELHPAVLERSGLPPRTCAVEIDLTALPIAESLPAPRVSPFPAVLQDVAVVVEATVPAATVEAALRSGAGELLEEIRLFDVFEGAQVGEGRKSLAFALRFRAADRTLTEDEASAARDAAVAAASAAVGAQLRA
>NZ_CP019572.1|WP_077041801.1|3102804_3103278_-|hypothetical-protein
MTDSQSVADGLSRLTDQELVRVIAAATAGRPALASIAAAANAIGGEIPPSPDVPIPAEVPFPADVPIPPDPTVVAGLPVPTAGPGLPTAMPAPDYTDSGVPTFDSVREKIETRAGTAAGATELDAESPAGRTVAEQWEERQKAAKDKLDEIRKTMDS
>NZ_CP019572.1|WP_083742936.1|3115670_3116060_+|hypothetical-protein
MIREIEDHHAVLERWLGTVADPALLTEFIGAHTADFSLVTTDGHVVSGDQLADSLRGAGGAVPGLRVVITAVEVQIATAEVAIARFTETHHTETGTGSGVRVVTAVLIPAPAARNGLRWRSVHETLRTD
>NZ_CP019572.1|WP_077041810.1|3116178_3118479_+|AAA-family-ATPase
MPDEALQHDEREREQQYVSMLYDRLDSLRDYAATRLRKVLLETGGTPQARSERESFSLMYTEDIAKYDAAENGLCFGRIDLDGQDPDEEAPDGDARRYIGRVGILDEANDYETLLLDWRAPLARPFYLATPAAPEGVTRRRHIRTRSRKVTGLGDEYLDLATAERAGALDGADGVAGESALLTALNAARTGHMNDIVETIQSEQDAIIRSEHKSVMVVQGGPGTGKTAVALHRAAYLLYTYRQQLAKSGVLIIGPNDTFLDYIGQVLPSLGETGVLLSTIGDLYPGVRATHQDSLECGEVKGSLAMVEVLKQAVRDRQETPPEPIRLRFDSYPISLDRKVVTRARGRARSSRRPHNLARPIFVSAVIEALADQHAARIGSNVLGGANLLSRDDIADIRDEMRGDPEVMAAIDGLWPQLTPQRVLADLLGSRKRLDTAARQLTAAQRELLHRTGVAGFSAADAPLLDELAELLGVDDAAERERADREWRAQIADAQGALDILTGSAPQDLEDELDPEILMAYDLIDAGQLAQRQDLRQSQTAAERAAADRTWTYGHVIVDEAQELSEMAWRMLMRRIPNRWMTLVGDTAQTGDPAGSSSWQTVLEPYVARRWKLTELTVNYRTPSEIMEVAQDVLALIDPDQTVPRSVRDSGTRPWARRVPEGDVAEQVRRLVAAETGPGLVAVLTSESGVDEYIDLISDSVSVLSVRQAKGLEFDAVLIVEPGQIVDESPRGMNDLYVALTRATQRLGVVHAHPLPDGLSKLSALE
>NZ_CP019572.1|WP_077041811.1|3118548_3119001_-|universal-stress-protein
MSAYRTIVVGTDGSDSSLKAVDKAASLAADAGAKLVIACAYYPADPKDIEAAQDTLREDAYQITGSAPTFEILRTARERAHAAGAADIVERAVVGAPVDSLLALIDEVEADLLVIGNRGLNTLTGRLLGSVPSDAARKSTSDVLIVHTVR
>NZ_CP019572.1|WP_077041812.1|3119149_3121963_+|alpha/beta-fold-hydrolase
MRIPRPQGIRTRIVVAVALVAVGAGAIAVTRSITEPPAPVTEDVAVTMPAGPSESDQVKIDARLYLPQHTPAPAVLLAHGFGGSKDSVAAQAGALAEDGFVVLAYSARGFGQSTGQISLNDPDREVADARGLVDWLAARGEVERDGPGDPRIGVAGGSYGGALALMLGGLDSRIDAIAAAITWNDLGHALFPNFGTIESPPPSTPAAAGYGGPGVLKRGWAGIFFGAGAGAAPSEAGAEAAEPRPEPGPCGRFALEFCREYAAAAVSGKPSRELLDLLRAHSPAAVAGAITAPTLLIQGEQDTLFGLDQSDATARQIAAAGTPVQVRWFPGGHDAGGEGTRFSESATSRFLSEQLRGSGEVDEEFVFSLPGTPTDQGRPSYRRMTAADYPGLVADAPPTPTIDLPLAGPASQSILRPPGAAPAALSAVPGLGSALSNLSAVGGPAADLVADPPGQSAAFATAPLDHPLTITGVSTARLTVTAASAPGDAVLFAKLYDVSPDGRRTLPGGAVSAIRLPDGPGPHPIAVALPGIAFQVPQGHHLEVVVATTDQAYANPLSPAQYSVALADDAGLLVPSVPAASSSRSLPIGPLIGIACILGGAALLVTIGVIRRRRRVAAEVDPALVDTPLVITDLRKTYDNGFRAVDGVSFTVRRGQVLGLLGPNGAGKTTTLRMLMGLITPTEGEIRVFGHRVTPGAPVLSRLGSFVEGSGLLPHLSGIDNLRLYWEATGRPAGEARMDEALEIADLGDAIERKVRSYSQGMRQRLAIAQAMLGLPDLMVLDEPTNGLDPPQIRTMREVLINYARTGRTVLVSSHLLAEVEQTCTDVVVMNHGRVISSGTVEELTSAEGATELRVDDPARAAVVLESVDGLGPVRLIEGENPRLRVDLGETDPAALVRTLVGAGIGVRGLSKTTRLEDVFLRLVDISDHREHDREDA
>NZ_CP019572.1|WP_077041813.1|3121959_3122871_+|ABC-transporter-permease
MTSHADGGAVTSHADGGAVMSHPDGRAAGYRATHTLPLRTELKRQFKRRRTHLALGFLALLPVILAIAFAIGGSDSSGSGGVDLIALGTTGGVNFTVFCLFMSVGFLLIVVVALFFGDSVASEASWSSLRYLLAIPVPRSRLLQQKAIVSAALSALAILILFGVALTLGTALYGTGPLVTPFGDSLGYREAVGRLLVALAFIAVNLTWVAGLALLLSVLTDAPLGAVGGTVMVSILVQILDQITALGHWRDYLPGHFSFAWTRLLAPTVDWHDIVVGSFSALAYAAVFGLLAWQRFAVKDITS
>NZ_CP019572.1|WP_077041814.1|3122887_3123754_-|hypothetical-protein
MDARTAGQAARTLDLIHGMSYFAPEVNESLVAAGLTPGRMCYFAGRSAAMGAVGAGVVAATFYNFNPEAIAPVIPTAWTQARPAAVVEARYRGVDAAMRRIYGAEAIGSSVFDEAASLAATAARAIPEAAGRPLFAGHAEIEWPSEPHLVLWHALTLLREYRGDGHIAALQTAGLSGIEALVSHTATGNGFQEQFARSRRGWSDEQWRGAVESLTDRGLLGDDGELTGAGWELRECVEELTDEMAVAPWAALGEDGAARLLELGTDLREVLVDQAVFPDGVYGSAVSR
>NZ_CP019572.1|WP_077041815.1|3123824_3125987_-|excinuclease-ABC-subunit-B
MAFASEHPVVAHSEFRPVGDIERSEARFEVVSPHQPAGDQPAAIAELEKRIRADEKNVVLLGATGTGKSATTAWLIEKLQRPTLVMAPNKTLAAQLANELREMLPNNAVEYFVSYYDYYQPEAYIAQTDTYIEKDSSINDDVERLRHSATSSLLSRRDVVVVASVSCIYGLGTPQSYLDRSIQLDVGVEVPRDALLRLLVDVQYNRNDLSFTRGSFRVRGDTVEIIPSYEELAIRIEFFGDEIEALYYLHPLTGDVVRQVDSLRIFPATHYVAGPERMERAVKDIEAELEERLADLEGRGKLLEAQRLRMRTNYDIEMIKQVGFCSGIENYSRHIDGRGAGTAPATLIDYFPEDFLLVIDESHVTVPQIGAMYEGDMSRKRNLVEFGFRLPSATDNRPLTWEEFTQRIGQTVYLSATPGKYELGQAGGEFVEQVIRPTGLVDPEVVVKPTKGQIDDLVHEIRERTDKDERVLVTTLTKKMAEDLTDYLLELGIRVRYLHSDIDTLRRVELLRQLRLGEYDVLVGINLLREGLDLPEVSLVAILDADKEGFLRSSTSLIQTIGRAARNVSGQVHMYADKITDSMAHAIEETDRRREKQIAYNREMGVDPQPLRKKIADILDQVYEEADDTAAGVDVGGSGRNASRGRRAQGEAGRAVSAGVYEGRDVKSMPRAELADLVKELTDQMMNAARELQFELAGRLRDEIQDLKKELRGMDAAGLS
>NZ_CP019572.1|WP_077041816.1|3126067_3126577_+|DUF402-domain-containing-protein
MEGEVHAPKIESFSLADKTNTDPKGFVRPVDHYRLEPWGLYMARTSDHAHFHYLESWLLPDLGLRASIFHYHPFHARDQNHYVDIGEFTAAPGVWRSEDHYLDLVVRTGRDTELLDVDELIAAHAAGLLDAATTELAIHRAVTAADGIAAHGHDLDAWLASVGMPISWR
>NZ_CP019572.1|WP_077041817.1|3126616_3127837_-|dephospho-CoA-kinase
MLRIGLTGGIGAGKSTVSRILTDLGAVVVDSDLVAREVVEPGTEGLAALVAEFGDSILQPDGRLDRPALAAVAFGDDEARARLNAVVHPLVGRRTTELVESAGADAVVVQDIPLLVEGRMGALFNLVLVVYVDAEERVRRLVELRGMPEHDARARLAAQATDDQRRAAADVWLDNSGPQGGLDAEVKALWEQRLVPFEENLRTGTVVRVRPVLAPADPTWPDQARRLIERLWLACGAGALRIDHVGSTSVPGLEAKDVIDVQITVSSIAAADALAGPLAAAGFPRIESITRDDPKPDYAIGGESDPALWDKRIHGGADPGRPVEISLRVDGWPGQRFALLLRDWLRADAAARAEFLEVKRVAVRWAAADAHTDEATVTYAAALAPWFDLGYQRAWEWAERSGWSLS
>NZ_CP019572.1|WP_027502000.1|3127961_3128174_+|hypothetical-protein
MQSHYILQGRHRLGIVNGVHCILIPEVATALAEHRRNWFTQMISPARHSFATMRKRTAVMQSAWAPAAVS

Crispr_ID: NZ_CP019572_2

• CRISPR_ID: NZ_CP019572_2
• CRISPR_location: 4545983-4546088
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 1 spacers

Consensus repeat of NZ_CP019572_2

Consensus_repeat	Method
CGACCGGCGGCTGGGCCTGGCCCGG	CRISPRCasFinder

spacers of NZ_CP019572_2

>2.1|4546008|56|NZ_CP019572|CRISPRCasFinder
TTCGAGAACATCCGAATCGGCGACGTGGCCCGGACCCTCGAAGGGTCCCTTCGGAC

CRISPR arrays and Neighbor proteins around NZ_CP019572_2

CRISPR arrays

The CRISPR arrays of NZ_CP019572_2

>merge|NZ_CP019572|2|4545983-4546088|CRISPRCasFinder
CGACCGGCGGCTGGGCCTGGCCCGGTTCGAGAACATCCGAATCGGCGACGTGGCCCGGACCCTCGAAGGGTCCCTTCGGACCGACCGGCGGCTCGGCCTGGCCCGG

>NZ_CP019572|2|2|4545983-4546088|CRISPRCasFinder
CGACCGGCGGCTGGGCCTGGCCCGG	TTCGAGAACATCCGAATCGGCGACGTGGCCCGGACCCTCGAAGGGTCCCTTCGGAC
CGACCGGCGGCTCGGCCTGGCCCGG

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
NZ_CP019572.1|WP_145982376.1|4547571_4548483_-|Ig-like-domain-repeat-protein	unknown	unknown	gnl|CDD|379864
NZ_CP019572.1|WP_077043865.1|4544035_4545103_+|ABC-transporter-permease	unknown	unknown	gnl|CDD|223650
NZ_CP019572.1|WP_077042636.1|4554654_4555467_-|CPBP-family-intramembrane-metalloprotease	unknown	unknown	gnl|CDD|376806
NZ_CP019572.1|WP_189636690.1|4541583_4542042_+|redox-sensitive-transcriptional-activator-SoxR	unknown	unknown	gnl|CDD|133385
NZ_CP019572.1|WP_083742975.1|4536872_4537748_+|SGNH/GDSL-hydrolase-family-protein	unknown	unknown	gnl|CDD|238861
NZ_CP019572.1|WP_077042635.1|4553018_4554449_-|Ig-like-domain-repeat-protein	unknown	unknown	gnl|CDD|379864
NZ_CP019572.1|WP_169310523.1|4557376_4558033_-|GNAT-family-N-acetyltransferase	unknown	unknown	gnl|CDD|224584
NZ_CP019572.1|WP_077042622.1|4534975_4535404_-|nuclear-transport-factor-2-family-protein	unknown	unknown	gnl|CDD|379275
NZ_CP019572.1|WP_077042634.1|4551181_4552960_+|long-chain-fatty-acid--CoA-ligase	unknown	unknown	gnl|CDD|223953
NZ_CP019572.1|WP_077042624.1|4539212_4539557_+|arsenate-reductase-(glutaredoxin)	unknown	unknown	gnl|CDD|239332
NZ_CP019572.1|WP_169310525.1|4542065_4542698_-|response-regulator-transcription-factor	unknown	unknown	gnl|CDD|225107
NZ_CP019572.1|WP_077042626.1|4540243_4541476_-|arginine-deiminase	unknown	unknown	gnl|CDD|234949
NZ_CP019572.1|WP_077042637.1|4555729_4556431_-|phosphoglycerate-mutase-family-protein	unknown	unknown	gnl|CDD|214859
NZ_CP019572.1|WP_077042631.1|4546638_4547487_-|SDR-family-oxidoreductase	unknown	unknown	gnl|CDD|181139
NZ_CP019572.1|WP_169310522.1|4549248_4549392_-|hypothetical-protein	unknown	unknown	unknown
NZ_CP019572.1|WP_145982374.1|4539626_4540070_-|hypothetical-protein	unknown	unknown	unknown
NZ_CP019572.1|WP_077042633.1|4548479_4548953_-|hypothetical-protein	unknown	unknown	unknown
NZ_CP019572.1|WP_077042629.1|4545153_4545837_+|ABC-transporter-ATP-binding-protein	unknown	unknown	gnl|CDD|213222
NZ_CP019572.1|WP_077042638.1|4556441_4557191_-|hypothetical-protein	unknown	unknown	gnl|CDD|340928
NZ_CP019572.1|WP_077042623.1|4537825_4539190_+|MFS-transporter	unknown	unknown	gnl|CDD|340886

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
NZ_CP019572.1|WP_145982376.1|4547571_4548483_-|Ig-like-domain-repeat-protein	gnl|CDD|379864	pfam16640, Big_3_5, Bacterial Ig-like domain (group 3). This family consists of bacterial domains with an Ig-like fold.	2.0087e-07
NZ_CP019572.1|WP_077043865.1|4544035_4545103_+|ABC-transporter-permease	gnl|CDD|223650	COG0577, SalY, ABC-type antimicrobial peptide transport system, permease component [Defense mechanisms].	1.13384e-07
NZ_CP019572.1|WP_077042636.1|4554654_4555467_-|CPBP-family-intramembrane-metalloprotease	gnl|CDD|376806	pfam02517, Abi, CAAX protease self-immunity. Members of this family are probably proteases (after a isoprenyl group is attached to the Cys residue in the C-terminal CAAX motif of a protein to attach it to the membrane, the AAX tripeptide being removed by one of the CAAX prenyl proteases). The family contains the CAAX prenyl protease. The proteins contain a highly conserved Glu-Glu motif at the amino end of the alignment. The alignment also contains two histidine residues that may be involved in zinc binding. While they are involved in membrane anchoring of proteins in eukaryotes, little is known about their function in prokaryotes. In some known bacteriocin loci, Abi genes have been found downstream of bacteriocin structural genes where they are probably involved in self-immunity. Investigation of the bacteriocin-like loci in the Gram positive bacteria locus from Lactobacillus sakei 23K confirmed that the bacteriocin-like genes (sak23Kalphabeta) exhibited antimicrobial activity when expressed in a heterologous host and that the associated Abi gene (sak23Ki) conferred immunity against the cognate bacteriocin. Interestingly, the immunity genes from three similar systems conferred a high degree of cross-immunity against each other's bacteriocins, suggesting the recognition of a common receptor. Site-directed mutagenesis demonstrated that the conserved motifs constituting the putative proteolytic active site of the Abi proteins are essential for the immunity function of Sak23Ki - thus a new concept in self-immunity.	0.000246089
NZ_CP019572.1|WP_189636690.1|4541583_4542042_+|redox-sensitive-transcriptional-activator-SoxR	gnl|CDD|133385	cd01110, HTH_SoxR, Helix-Turn-Helix DNA binding domain of the SoxR transcription regulator. Helix-turn-helix (HTH) transcriptional regulator SoxR. The global regulator, SoxR, up-regulates gene expression of another transcription activator, SoxS, which directly stimulates the oxidative stress regulon genes in E. coli. The soxRS response renders the bacterial cell resistant to superoxide-generating agents, macrophage-generated nitric oxide, organic solvents, and antibiotics. The SoxR proteins share the N-terminal DNA binding domain with other transcription regulators of the MerR superfamily that promote transcription by reconfiguring the unusually long spacer between the -35 and -10 promoter elements. They also harbor a regulatory C-terminal domain containing an iron-sulfur center.	1.00368e-81
NZ_CP019572.1|WP_083742975.1|4536872_4537748_+|SGNH/GDSL-hydrolase-family-protein	gnl|CDD|238861	cd01823, SEST_like, SEST_like. A family of secreted SGNH-hydrolases similar to Streptomyces scabies esterase (SEST), a causal agent of the potato scab disease, which hydrolyzes a specific ester bond in suberin, a plant lipid. The tertiary fold of this enzyme is substantially different from that of the alpha/beta hydrolase family and unique among all known hydrolases; its active site closely resembles two of the three components of typical Ser-His-Asp(Glu) triad from other serine hydrolases, but may lack the carboxylic acid.	2.86127e-50
NZ_CP019572.1|WP_077042635.1|4553018_4554449_-|Ig-like-domain-repeat-protein	gnl|CDD|379864	pfam16640, Big_3_5, Bacterial Ig-like domain (group 3). This family consists of bacterial domains with an Ig-like fold.	5.05168e-08
NZ_CP019572.1|WP_169310523.1|4557376_4558033_-|GNAT-family-N-acetyltransferase	gnl|CDD|224584	COG1670, RimL, Acetyltransferases, including N-acetylases of ribosomal proteins [Translation, ribosomal structure and biogenesis].	3.19434e-33
NZ_CP019572.1|WP_077042622.1|4534975_4535404_-|nuclear-transport-factor-2-family-protein	gnl|CDD|379275	pfam13577, SnoaL_4, SnoaL-like domain. This family contains a large number of proteins that share the SnoaL fold.	2.6186e-33
NZ_CP019572.1|WP_077042634.1|4551181_4552960_+|long-chain-fatty-acid--CoA-ligase	gnl|CDD|223953	COG1022, FAA1, Long-chain acyl-CoA synthetases (AMP-forming) [Lipid metabolism].	4.72044e-170
NZ_CP019572.1|WP_077042624.1|4539212_4539557_+|arsenate-reductase-(glutaredoxin)	gnl|CDD|239332	cd03034, ArsC_ArsC, Arsenate Reductase (ArsC) family, ArsC subfamily; arsenic reductases similar to that encoded by arsC on the R733 plasmid of Escherichia coli. E. coli ArsC catalyzes the reduction of arsenate [As(V)] to arsenite [As(III)], the first step in the detoxification of arsenic, using reducing equivalents derived from glutathione (GSH) via glutaredoxin (GRX). ArsC contains a single catalytic cysteine, within a thioredoxin fold, that forms a covalent thiolate-As(V) intermediate, which is reduced by GRX through a mixed GSH-arsenate intermediate. This family of predominantly bacterial enzymes is unrelated to two other families of arsenate reductases which show similarity to low-molecular-weight acid phosphatases and phosphotyrosyl phosphatases.	5.68191e-51
NZ_CP019572.1|WP_169310525.1|4542065_4542698_-|response-regulator-transcription-factor	gnl|CDD|225107	COG2197, CitB, Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain [Signal transduction mechanisms / Transcription].	2.12555e-70
NZ_CP019572.1|WP_077042626.1|4540243_4541476_-|arginine-deiminase	gnl|CDD|234949	PRK01388, PRK01388, arginine deiminase; Provisional.	0
NZ_CP019572.1|WP_077042637.1|4555729_4556431_-|phosphoglycerate-mutase-family-protein	gnl|CDD|214859	smart00855, PGAM, Phosphoglycerate mutase family. Phosphoglycerate mutase (PGAM) and bisphosphoglycerate mutase (BPGM) are structurally related enzymes that catalyse reactions involving the transfer of phospho groups between the three carbon atoms of phosphoglycerate... Both enzymes can catalyse three different reactions with different specificities, the isomerization of 2-phosphoglycerate (2-PGA) to 3-phosphoglycerate (3-PGA) with 2,3-diphosphoglycerate (2,3-DPG) as the primer of the reaction, the synthesis of 2,3-DPG from 1,3-DPG with 3-PGA as a primer and the degradation of 2,3-DPG to 3-PGA (phosphatase activity). In mammals, PGAM is a dimeric protein with two isoforms, the M (muscle) and B (brain) forms. In yeast, PGAM is a tetrameric protein.	2.75613e-32
NZ_CP019572.1|WP_077042631.1|4546638_4547487_-|SDR-family-oxidoreductase	gnl|CDD|181139	PRK07832, PRK07832, SDR family oxidoreductase.	9.16622e-177
NZ_CP019572.1|WP_077042629.1|4545153_4545837_+|ABC-transporter-ATP-binding-protein	gnl|CDD|213222	cd03255, ABC_MJ0796_LolCDE_FtsE, ATP-binding cassette domain of the transporters involved in export of lipoprotein and macrolide, and cell division protein. This family is comprised of MJ0796 ATP-binding cassette, macrolide-specific ABC-type efflux carrier (MacAB), and proteins involved in cell division (FtsE), and release of lipoproteins from the cytoplasmic membrane (LolCDE). They are clustered together phylogenetically. MacAB is an exporter that confers resistance to macrolides, while the LolCDE system is not a transporter at all. An FtsE null mutants showed filamentous growth and appeared viable on high salt medium only, indicating a role for FtsE in cell division and/or salt transport. The LolCDE complex catalyzes the release of lipoproteins from the cytoplasmic membrane prior to their targeting to the outer membrane.	1.08064e-90
NZ_CP019572.1|WP_077042638.1|4556441_4557191_-|hypothetical-protein	gnl|CDD|340928	cd17370, MFS_MJ1317_like, MJ1317 and similar transporters of the Major Facilitator Superfamily. This family is composed of Methanocaldococcus jannaschii MFS-type transporter MJ1317, Mycobacterium bovis protein Mb2288, and similar proteins. They are uncharacterized transporters belonging to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.	0.00346126
NZ_CP019572.1|WP_077042623.1|4537825_4539190_+|MFS-transporter	gnl|CDD|340886	cd17328, MFS_spinster_like, Protein spinster and spinster homologs of the Major Facilitator Superfamily of transporters. The protein spinster family includes Drosophila protein spinster, its vertebrate homologs, and similar proteins. Humans contain three homologs called protein spinster homologs 1 (SPNS1), 2 (SPNS2), and 3 (SPNS3). Protein spinster and its homologs may be sphingolipid transporters that play central roles in endosomes and/or lysosomes storage. SPNS2 is also called sphingosine 1-phosphate (S1P) transporter and is required for migration of myocardial precursors. S1P is a secreted lipid mediator that plays critical roles in cardiovascular, immunological, and neural development and function. The spinster-like family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.	1.79401e-81

Protein sequences

>NZ_CP019572.1|WP_077042629.1|4545153_4545837_+|ABC-transporter-ATP-binding-protein
MSITLKNTVLTYPDGDTRLRALDDVSLTVERGQLAAITGPSGSGKSSLLAVAGLLITPDSGTVTIDGTDVTGLDRTARTTVRRQQLGFVFQQANLMASLTATEQLLMITHLGGGSVRGQRARARDLLSTLGLEKQLDRRPHQLSGGQRQRVAIARSLMNDPSVLLVDEPTSALDEDRSREIVELLAALSHERNVAIVMVTHDLSQLDLVDGVYEMHSGALSTAAVSA
>NZ_CP019572.1|WP_077043865.1|4544035_4545103_+|ABC-transporter-permease
MFLSIRDLRFAKGRFALMSAVLFLISLMVVMLSGLTAGLGNQSIAAIQSVGADRFAFGEPAEGQKVAFTDSQVTEAQRAQLASTPGVDEASILGIAPVRITADGRDVSASGFGVAGDSFAAPAQLHTGEIAVDRKLADDNGWAVGTTVDIAGHPLTIGTLVDDSYYSHQSVVWLDLADWRLLPTAGDSEGTTIALRTSSDFDAAAAESLTGTAITDEKGALNAIGAYSSERGSLLLMQGMLLAISALVVGAFFTIWTIQRGQDLAVLKAVGASTWYLLVDALGQGLIVLTLGAGLGTLAAIGLGSVAAAAVPFTLTVTGTLVPFFALIALGMLGAAAALTRIVKVDPLTALGAAR
>NZ_CP019572.1|WP_169310525.1|4542065_4542698_-|response-regulator-transcription-factor
MTGPRLRLLLVDDHPVVRAGLRALLSSHEGLDVVAEAASGEQALELVETTRPDVVLMDLRLGAGMDGAQSTEAIMARPDPPRVVVLTTYDTDADILRAVEAGAAGYLLKDSDPQVLLDAVWAASRGETVLNPDVAQRLYRRIREPRVDLSARETEIVRLVADGLSNRAIAKQLFVTEATVKSHLVHVFSKLGVDSRTAAVAAARERGLLR
>NZ_CP019572.1|WP_189636690.1|4541583_4542042_+|redox-sensitive-transcriptional-activator-SoxR
MPDWKAKQLTPGQLSERSGVAVSALHFYEREGLIESRRTSGNQRRYARDTLRRVAFIRISQQVGIPLAEIREALDTLPEGRTPTRADWERLSTSWHADLDRRIEQLTRLRDNLTGCIGCGCLSMNSCALANPHDRLATAGPGARVLDVRLRC
>NZ_CP019572.1|WP_077042626.1|4540243_4541476_-|arginine-deiminase
MERSVVGNEGPSVLGANSEVGTLRTVLLHRPGDELKRLTPRNNDQLLFDGLPWVDRAQEEHDAFAALLRSRGVEVLLLSEVLTETLTVSGAARMQGISAAVDPRRLGHPLAQELAAHLRTVEPAALATILTAGMTFDELPLGPGGASLVRRMHHGGDFVIEPLPNLLFTRDSSFWIGDRVAITALALPARVREASLTDLIYAHHPRFLGVRRAYESHTAPVEGGDVLLLAPGVVAVGVGERTTPAGAEALARSVFEDGLAHSVLVVPIAQSRASMHLDTVCTMVDVDAVVMYPAIQDSLSAFTIHREDVGVSIRGAEPFLKAAADAMGIGKLRVIDTGLDNVTAEREQWDDGNNTLALEPGVVVAYERNVVTNSRLEASGIEVLRISGSELGSGRGGPRCMSCPVSRDLL
>NZ_CP019572.1|WP_145982374.1|4539626_4540070_-|hypothetical-protein
MSIASVAVAGGVLAGAGVAFAGTPVITHDGGFQVGSAGLAPGTYESTGPTTLGAPCTWTRLSLTNGQPTVIEYMQTNGPSAVTLQATDSTFVTYNCGVWTPEEVVVPETPVVTEPEPVEAEEEDASGSLDFGSMDLGSVDFLGSAGS
>NZ_CP019572.1|WP_077042624.1|4539212_4539557_+|arsenate-reductase-(glutaredoxin)
MADATIYHNQRCSTSRTVLKLIEEAGIEPTIVKYLETPPSRDGLRKLLDDAGLKPSEAIRKKEALYKELGLAEASEDELLEAMVENPILIERPIVVTAKGTVLARPAERVSTIL
>NZ_CP019572.1|WP_077042623.1|4537825_4539190_+|MFS-transporter
MSVHEPTVAPSALPAATTKVVSGALIALFLANLVNFFDRAIPSVVAEPIKAEFSLSDLQLGLIMSAFTLVYAVAGLPLGRLADRGNRPKIIGAGLLLWSGLTAATGAAGNYVLFILARLGVGVGEASFTPAANSMIGDMYPAERRSRALGVFMLGLPVGLMLAYFTVGKITEAFGSWRAPFFVAAVPGVLLALVMFRMRDPERGGSEHTAAGAVLTDDAAITRPIRRLLSIPTLRWLILAGLTFNFAAYAVNSFVVPLLMRQYELKLTPAAAVTGIIVGLTGLIGLLAGARLADRAATTSPNARLTIGVVSCAVAAPLTALALMQNGGSVVAFTALFAAGWLLAYGFYISVYPAIHDVVVPRLRATATALFFAAMYLLGGFSGPVVVGAISDWFAQRALDAAGTGTLAQYSGQGLNSAMYLIPLMLVLTAVFIYLATRTIAADSTRMRRSLESR
>NZ_CP019572.1|WP_083742975.1|4536872_4537748_+|SGNH/GDSL-hydrolase-family-protein
MIRRATRSGATAAATLCLLAAAAAGNAGAAPLQPGAIDYVALGDSAAAGPLLPDQDMSAPGCLRSRDNYPAVVAAALGATLTDVTCSSARTAHITDTRQGTFAGAVPPQLDALSPDTDLVTVTIGANDFNLFSTALSCTNPLPQPTGTSCRDKFTAGGRNQQRDLIRDWAPTWGAMLDKIRERAPHAEIAVVGYGTYLRPGGCPAQQPIWPQDADYLQSVMADANEAMAAEAARRDIRFIDIRQATSGHDICAGPTQAHYAGLVPAEPSVPLHPTVLGMQAIGAHVAGELR
>NZ_CP019572.1|WP_077042622.1|4534975_4535404_-|nuclear-transport-factor-2-family-protein
MDLQTISDRIEIQDLFTRYAHAVDSKDWDLYRTVFIPDAHIDYSTAGGPVGDLEFVVAQLATMLDLFAQTQHFVSNIDVELDGDRARVRAMFFNPMIVKKDRQFFCGGWYNHELVRTPEGWRSRSLVEESAWFDGVAEAFAD
>NZ_CP019572.1|WP_077042631.1|4546638_4547487_-|SDR-family-oxidoreductase
MREGHREGPAGKKCLITGAGSGIGRATALAAAAEGAELFLTDINEVGLAETVVMAGSAVTASKALDVSDYDAVTAWATEIDGAHGAMDVVMNVAGISAWGTVENLEHRHWKSMVDVNLMGPIHIIENFVPPMVRAGRGGHLVNVSSAAGLLALPWHAAYSASKFGLRGVSEVLRFDLKRHGIGVSLVVPGGVKTPLVGTVQIAGVDRDDPRIRKLVHRFEKRAVSPEKVAECIVRGIKKDRYLVYTSNDIRFGYWWARKFAPPYEFVMQKANDQFSKLLPPV
>NZ_CP019572.1|WP_145982376.1|4547571_4548483_-|Ig-like-domain-repeat-protein
MTKNVSTTVLEPVGPLKAGVESTLKSKVLGGRAGDLVKFFSGTNEIGSGALDAAGAASFKWKPAANQAGPHDLTAKFLATPFATESVSAVQSVTVDPGDQTTQTTLIMPSSSPINVDVIIEAQVSPFPGAGTLTFKNGLTVLGTAPVGADGKASVTQNFTVPGNKTITAVFSGAPGFAGSTSVAQTLNITDPGAADVATTTTLNVPAIAQKGVFVFLRANVAPRANGGTVQFFSGAEPLGNPATVKDGQASLSYLFTTSGVRDVRAVYSGAPGYLGSEASTTVTVQDEPVTGGGGGSLPNFGS
>NZ_CP019572.1|WP_077042633.1|4548479_4548953_-|hypothetical-protein
MRRTIAMGGAFAVTAGFAVTLGAGGASAAPATITWADGGKNFTRTISNVAPAEGEVVTVSTTIAGTDTVNWFEDHRNTCLTYKKDSAKVAGLPVPLASNGAGLLRVAGSWDASTSPTFEFQYVIGANCAREVALNTGIKYGAVASSGRTSPSVRRTW
>NZ_CP019572.1|WP_169310522.1|4549248_4549392_-|hypothetical-protein
MTDNELRKAIRTLRDRADEARRHGDPEDADSIEKTIRDYQDEMSTRL
>NZ_CP019572.1|WP_077042634.1|4551181_4552960_+|long-chain-fatty-acid--CoA-ligase
MNTSNASPQTLCEAFQTTAATYPDAVALRTPGDGVVITWRLYAERVRRIAAGLAKLGVRHRDTVGLMLTNRPEFHLLDTAALHAGATPFSVYNMLAAEQLNYVLTNAGNKVMICEEQFLPVLRDAIAGTAVERIVCVDGDAEDTTSLRDLEANGDRDFDFEASWRAVEPDDLLTIIYTSGTTGPPKGVELTHANILAEYDAVRDIANLGPADRYLSYLPDAHIANRLFHYGSLKGGIQITTLADAKQAVTALPDVRPTVLLAVPQVWYKLKAAIELALIDEPSTAKRRLADWAIGLGRRKVRLESDQAPVPRTLRIQHAVADRLVLGSVRAKLGLDQVRVAISAAAALSPDTLEFVLGLGIPCVEAWGLSETTGGVTVNRPDAIRIGTVGRAVKGAVLRLAEDGELLVSGPVVMKGYRNDPAKTAEALDPDGWLHTGDIATIDADGYVTIVDRKKELIINAGGKNMSPSNIEGTIRAASSLIGGAVVIGNDRPYVVALLTLDPDAAAAFATRNGIHNGSPGDLASNPVVRAEIDSAIASANAKLSRVEQVKKFTLLAAIWEPGGDEFTPTMKLRRKPIDAKYAREIEELYAG
>NZ_CP019572.1|WP_077042635.1|4553018_4554449_-|Ig-like-domain-repeat-protein
MSDKSIRRVVGGAGVLALSAGFAATVGAGVAGAAPASVTWDDGSERITRTISDTTPAEGDIITSTTQFDRTGGVVEYIYEVKDVHPACLTYVDGSAKVDGSPRGLDSQGADFAKVKGSSIEWPLYPHISPNLRTFEFSYKVGANCDRNVPLMTTVHYGGSLGSGTYQDKGPTVSVRINTSTTALATPANVQVGQSVPLKATVTGGAEGNPVEFFDGTVKIGQAALNGAGEATFNWTPATRGAHGLSAKFLATPRAEGSQSPVQTVQVSQADAVSSTVLAPISGAEVGRSTVLKATVSPTGAGGTVAFKDGAATLADVPVDANGVATYTWVPSTAGSHSIAATFSGRSGVTGSTANATVTVAEAPAGNTNSTTVLTLGGAPKVGAAQTISAQVTPGDAGGTVTFKDGDIVIGTAQVNAGGQASLSWTPANEGQRIITAEYSGAGTVNASADQESVVVTGTGGGTPGGAGSLGSIFGS
>NZ_CP019572.1|WP_077042636.1|4554654_4555467_-|CPBP-family-intramembrane-metalloprotease
MNAPEHEAAPRITGRHLSYLAFAVVVIVYLAIIQFGGHLIAGWGDEGDTLTTRGVVFTMVIPLGLALAFTYGVIAALGWWRPAIKDDRPVNRWVWIVPVILLASILLGIDYSALSEKGWLFIIALLVATQFVGWGEEGMFRGIGVTMLRNHGLREGRVALWSSLIFGAVHLSNIFGHGVSAIPQAIMVSLAGYFFYLTRRVSGSNALNSVLHGLFDFALLSGSAIVLDQHGYIGSFAPILAYPILAIILLVKRRDIEPSHRTDHAVAPRT
>NZ_CP019572.1|WP_077042637.1|4555729_4556431_-|phosphoglycerate-mutase-family-protein
MADGAGPERIVLLRHGESEANIDPGIYERVPDYRIPLTARGVEQSRVAGERLREQFDGQKVCVYVSPYLRAYQTLEALGIDDLIERRIEEPRLREQDWANYQDPDEIARQTGLRNTYGHFFYRFNEGESGADVFDRVSTFMETLYRHRAKPGFPPNTLVVTHGLTMRLFCMRWFHWSVEYFESLNNPGNAETRTLVRQADGRFALEQPFDQWRAVPEGQTILDTTRAGVAGPE
>NZ_CP019572.1|WP_077042638.1|4556441_4557191_-|hypothetical-protein
MPNSILWIALVGMWLFVLLPMQVKRRPRIRQTTDAALATRVLDRDQLARQQHRGPASGHRSDPNWRREPTRPRYAEDRMDTQVEVDELDDERAEAREYVPQRRGRGGFDPEADALARAARYGFRQRAVLGLILLAIMSAALALIISPMLWWVAAAAFVGLGGYLAYMRRQVQFEQEIRRRRMARLSRSRLGVESREDDELRLIPSRLRRPGAVVLEVDDEDPVFEHLEHYDVRFEEEAAPQEMRRVVGQ
>NZ_CP019572.1|WP_169310523.1|4557376_4558033_-|GNAT-family-N-acetyltransferase
MGTSLRKYSIHPGWPAHLGPLTVGAGRVTLRPIRLRDAAAWSRIRIRDQEHLEPWEPTGEGPWEDRHHIGNWPGLCASLRSEARKGRMVPLVIELDGKFCGQLTIGNVVRGALRSAWIGYWVTREVGGQGVATAAVALGLDHSFGALGLHRVEATVRPENLASQAVLRNVGFREEGLLRRYLDVDGAWRDHLLVAMTIEEVEGTVSDRLVRAGKAAWG

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity
NZ_CP019572_1	1.1|3115428|30|NZ_CP019572|CRISPRCasFinder	3115428-3115457	30	NZ_CP044425	Paracoccus pantotrophus strain DSM 2944 plasmid pPAN2, complete sequence	458514-458543	6	0.8
NZ_CP019572_1	1.1|3115428|30|NZ_CP019572|CRISPRCasFinder	3115428-3115457	30	NZ_CP030074	Streptomyces sp. ZFG47 plasmid unnamed1, complete sequence	396836-396865	6	0.8
NZ_CP019572_1	1.1|3115428|30|NZ_CP019572|CRISPRCasFinder	3115428-3115457	30	NZ_LR594663	Variovorax sp. RA8 plasmid 2	395799-395828	7	0.767
NZ_CP019572_1	1.1|3115428|30|NZ_CP019572|CRISPRCasFinder	3115428-3115457	30	MH155867	Gordonia phage Easley, complete genome	33014-33043	7	0.767
NZ_CP019572_1	1.1|3115428|30|NZ_CP019572|CRISPRCasFinder	3115428-3115457	30	NZ_CP049749	Rhodococcus fascians A21d2 plasmid pA21d2, complete sequence	130429-130458	7	0.767
NZ_CP019572_1	1.1|3115428|30|NZ_CP019572|CRISPRCasFinder	3115428-3115457	30	NC_013857	Azospirillum sp. B510 plasmid pAB510c, complete sequence	328270-328299	7	0.767
NZ_CP019572_1	1.1|3115428|30|NZ_CP019572|CRISPRCasFinder	3115428-3115457	30	NZ_CP045549	Streptomyces sp. SYP-A7193 plasmid unnamed2, complete sequence	7431-7460	8	0.733
NZ_CP019572_1	1.1|3115428|30|NZ_CP019572|CRISPRCasFinder	3115428-3115457	30	NZ_CP019604	Croceicoccus marinus strain E4A9 plasmid pCME4A9II, complete sequence	3239-3268	8	0.733
NZ_CP019572_1	1.1|3115428|30|NZ_CP019572|CRISPRCasFinder	3115428-3115457	30	NZ_CP016083	Streptomyces sp. SAT1 plasmid unnamed3, complete sequence	278153-278182	8	0.733
NZ_CP019572_1	1.1|3115428|30|NZ_CP019572|CRISPRCasFinder	3115428-3115457	30	CP000662	Rhodobacter sphaeroides ATCC 17025 plasmid pRSPA01, complete sequence	239523-239552	8	0.733
NZ_CP019572_1	1.1|3115428|30|NZ_CP019572|CRISPRCasFinder	3115428-3115457	30	NZ_LR134418	Legionella adelaidensis strain NCTC12735 genome assembly, plasmid: 9	65269-65298	8	0.733
NZ_CP019572_1	1.1|3115428|30|NZ_CP019572|CRISPRCasFinder	3115428-3115457	30	NC_007336	Cupriavidus pinatubonensis JMP134 megaplasmid, complete sequence	188696-188725	9	0.7

1. spacer 1.1|3115428|30|NZ_CP019572|CRISPRCasFinder matches to NZ_CP044425 (Paracoccus pantotrophus strain DSM 2944 plasmid pPAN2, complete sequence) position: , mismatch: 6, identity: 0.8

acacgccccggaactcctcgcgctcgcccg	CRISPR spacer
tgatgcccaggaactcctcgcgctcggcgg	Protospacer
  *.**** ***************** * *

2. spacer 1.1|3115428|30|NZ_CP019572|CRISPRCasFinder matches to NZ_CP030074 (Streptomyces sp. ZFG47 plasmid unnamed1, complete sequence) position: , mismatch: 6, identity: 0.8

acacgccccggaactcctcgcgctcgcccg	CRISPR spacer
tcgcgccccgcacctcctcgcgctcgcgct	Protospacer
 *.******* * ************** * 

3. spacer 1.1|3115428|30|NZ_CP019572|CRISPRCasFinder matches to NZ_LR594663 (Variovorax sp. RA8 plasmid 2) position: , mismatch: 7, identity: 0.767

acacgccccggaactcctcgcgctcgcccg	CRISPR spacer
cgggacgccggaactcctggcgctcgcccg	Protospacer
  . .* *********** ***********

4. spacer 1.1|3115428|30|NZ_CP019572|CRISPRCasFinder matches to MH155867 (Gordonia phage Easley, complete genome) position: , mismatch: 7, identity: 0.767

acacgccccggaactcctcgcgctcgcccg	CRISPR spacer
aaccggaccggaactcctcgcgcacgccgt	Protospacer
*  **  **************** ****  

5. spacer 1.1|3115428|30|NZ_CP019572|CRISPRCasFinder matches to NZ_CP049749 (Rhodococcus fascians A21d2 plasmid pA21d2, complete sequence) position: , mismatch: 7, identity: 0.767

acacgccccggaactcctcgcgctcgcccg	CRISPR spacer
gcaacgaccggatctgctcgcgctcgcccg	Protospacer
.**    ***** ** **************

6. spacer 1.1|3115428|30|NZ_CP019572|CRISPRCasFinder matches to NC_013857 (Azospirillum sp. B510 plasmid pAB510c, complete sequence) position: , mismatch: 7, identity: 0.767

acacgccccggaactcctcgcgctcgcccg	CRISPR spacer
tcagccgccggaactcctcgccctcgccga	Protospacer
 **  * ************** ****** .

7. spacer 1.1|3115428|30|NZ_CP019572|CRISPRCasFinder matches to NZ_CP045549 (Streptomyces sp. SYP-A7193 plasmid unnamed2, complete sequence) position: , mismatch: 8, identity: 0.733

acacgccccggaactcctcgcgctcgcccg	CRISPR spacer
cagtgcccgggaactcgtcgcgctcgccga	Protospacer
  ..**** ******* *********** .

8. spacer 1.1|3115428|30|NZ_CP019572|CRISPRCasFinder matches to NZ_CP019604 (Croceicoccus marinus strain E4A9 plasmid pCME4A9II, complete sequence) position: , mismatch: 8, identity: 0.733

acacgccccggaactcctcgcgctcgcccg	CRISPR spacer
tcttgcgccggaactcctcgcgctctcgtt	Protospacer
 * .** ****************** * . 

9. spacer 1.1|3115428|30|NZ_CP019572|CRISPRCasFinder matches to NZ_CP016083 (Streptomyces sp. SAT1 plasmid unnamed3, complete sequence) position: , mismatch: 8, identity: 0.733

acacgccccggaactcctcgcgctcgcccg	CRISPR spacer
agacgccccggaactcctcgggcgacaggg	Protospacer
* ****************** **      *

10. spacer 1.1|3115428|30|NZ_CP019572|CRISPRCasFinder matches to CP000662 (Rhodobacter sphaeroides ATCC 17025 plasmid pRSPA01, complete sequence) position: , mismatch: 8, identity: 0.733

acacgccccggaactcctcgcgctcgcccg	CRISPR spacer
acacgccgcggacctcctcgcgcagcggca	Protospacer
******* **** **********     *.

11. spacer 1.1|3115428|30|NZ_CP019572|CRISPRCasFinder matches to NZ_LR134418 (Legionella adelaidensis strain NCTC12735 genome assembly, plasmid: 9) position: , mismatch: 8, identity: 0.733

acacgccccggaactcctcgcgctcgcccg	CRISPR spacer
tagtgccccagaactcctcccgctcgcgca	Protospacer
  ..*****.********* ******* *.

12. spacer 1.1|3115428|30|NZ_CP019572|CRISPRCasFinder matches to NC_007336 (Cupriavidus pinatubonensis JMP134 megaplasmid, complete sequence) position: , mismatch: 9, identity: 0.7

acacgccccggaactcctcgcgctcgcccg	CRISPR spacer
cgatagcccggaattcctcgcgctcgcgac	Protospacer
  *.. *******.*************