CRISPRimmunity

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Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Self targeting spacer number	Target MGE spacer number	Prophage number	Anti-CRISPR protein number
NZ_CP020352	Bacillus paralicheniformis strain MDJK30 chromosome, complete genome	4 crisprs	csa3,WYL,DinG,cas3,DEDDh	5	11	4	0

Results visualization

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CRISPR-Cas detection and classification

Crispr_ID: NZ_CP020352_1

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

NZ_CP020352_1

57448-57529

Unclear

Consensus_repeat	Method
TGCACGGGCGCATGCATTTGTAC	CRISPRCasFinder

1 spacers

cas3

The CRISPR arrays of NZ_CP020352_1

>merge|NZ_CP020352|1|57448-57529|CRISPRCasFinder
TGCACGGGCGCATGCATTTGTACTTGTAAGGATGACCGCATGCTAAAGGCGCGGCGTCCTGCGCGGGCGCATGCATTTGTAC

>NZ_CP020352|1|1|57448-57529|CRISPRCasFinder
TGCACGGGCGCATGCATTTGTAC	TTGTAAGGATGACCGCATGCTAAAGGCGCGGCGTCC
TGCGCGGGCGCATGCATTTGTAC

Protein	Signature genes	Signature genes Name	Protein_function
NZ_CP020352.1\|WP_003178189.1\|57114_57300_+\|small,-acid-soluble-spore-protein,-alpha/beta-type	unknown	unknown	gnl\|CDD\|365990
NZ_CP020352.1\|WP_023855980.1\|55591_56473_+\|sporulation-peptidase-YabG	unknown	unknown	gnl\|CDD\|274322
NZ_CP020352.1\|WP_020449810.1\|62947_63574_+\|50S-ribosomal-protein-L25/general-stress-protein-Ctc	unknown	unknown	gnl\|CDD\|235534
NZ_CP020352.1\|WP_020449808.1\|60525_61896_+\|bifunctional-UDP-N-acetylglucosamine-diphosphorylase/glucosamine-1-phosphate-N-acetyltransferase-GlmU	unknown	unknown	gnl\|CDD\|184643
NZ_CP020352.1\|WP_020449807.1\|59372_59750_+\|RidA-family-protein	unknown	unknown	gnl\|CDD\|129116
NZ_CP020352.1\|WP_003178210.1\|64313_64544_+\|anti-sigma-F-factor-Fin-family-protein	unknown	unknown	gnl\|CDD\|371314
NZ_CP020352.1\|WP_041817399.1\|47829_48705_+\|16S-rRNA-(cytidine(1402)-2'-O)-methyltransferase	unknown	unknown	gnl\|CDD\|223390
NZ_CP020352.1\|WP_020449801.1\|52636_53833_+\|DUF348-domain-containing-protein	unknown	unknown	gnl\|CDD\|226111
NZ_CP020352.1\|WP_020449800.1\|51624_52392_+\|TatD-family-hydrolase	unknown	unknown	gnl\|CDD\|223162
NZ_CP020352.1\|WP_020449812.1\|64608_68142_+\|transcription-repair-coupling-factor	cas3	cd09639_cas3_CAS-I	gnl\|CDD\|224118
NZ_CP020352.1\|WP_020449803.1\|54553_55432_+\|16S-rRNA-(adenine(1518)-N(6)/adenine(1519)-N(6))--dimethyltransferase-RsmA	unknown	unknown	gnl\|CDD\|234708
NZ_CP020352.1\|WP_020449809.1\|61916_62867_+\|ribose-phosphate-diphosphokinase	unknown	unknown	gnl\|CDD\|234929
NZ_CP020352.1\|WP_003178198.1\|59930_60224_+\|septation-protein-SpoVG	unknown	unknown	gnl\|CDD\|237321
NZ_CP020352.1\|WP_085959887.1\|48754_49045_-\|AbrB/MazE/SpoVT-family-DNA-binding-domain-containing-protein	unknown	unknown	gnl\|CDD\|224913
NZ_CP020352.1\|WP_020449806.1\|58521_59355_+\|pur-operon-repressor	unknown	unknown	gnl\|CDD\|236414
NZ_CP020352.1\|WP_020449799.1\|49552_51541_+\|methionine--tRNA-ligase	unknown	unknown	gnl\|CDD\|237028
NZ_CP020352.1\|WP_003178187.1\|56697_56958_+\|hypothetical-protein	unknown	unknown	gnl\|CDD\|226872
NZ_CP020352.1\|WP_025809574.1\|57595_58465_+\|4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol-kinase	unknown	unknown	gnl\|CDD\|234652
NZ_CP020352.1\|WP_020449802.1\|54000_54561_+\|ribonuclease-M5	unknown	unknown	gnl\|CDD\|273019
NZ_CP020352.1\|WP_020449811.1\|63690_64257_+\|aminoacyl-tRNA-hydrolase	unknown	unknown	gnl\|CDD\|235457

Protein	Function_ID	Function_description	E-value
NZ_CP020352.1\|WP_003178189.1\|57114_57300_+\|small,-acid-soluble-spore-protein,-alpha/beta-type	gnl\|CDD\|365990	pfam00269, SASP, Small, acid-soluble spore proteins, alpha/beta type.	8.31803e-05
NZ_CP020352.1\|WP_023855980.1\|55591_56473_+\|sporulation-peptidase-YabG	gnl\|CDD\|274322	TIGR02855, Sporulation-specific_protease_YabG, sporulation peptidase YabG. Members of this family are the protein YabG, demonstrated for Bacillus subtilis to be an endopeptidase able to release N-terminal peptides from a number of sporulation proteins, including CotT, CotF, and SpoIVA. It appears to be expressed under control of sigma-K. [Cellular processes, Sporulation and germination].	0
NZ_CP020352.1\|WP_020449810.1\|62947_63574_+\|50S-ribosomal-protein-L25/general-stress-protein-Ctc	gnl\|CDD\|235534	PRK05618, PRK05618, 50S ribosomal protein L25/general stress protein Ctc; Reviewed.	3.34025e-67
NZ_CP020352.1\|WP_020449808.1\|60525_61896_+\|bifunctional-UDP-N-acetylglucosamine-diphosphorylase/glucosamine-1-phosphate-N-acetyltransferase-GlmU	gnl\|CDD\|184643	PRK14354, glmU, bifunctional UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase GlmU.	0
NZ_CP020352.1\|WP_020449807.1\|59372_59750_+\|RidA-family-protein	gnl\|CDD\|129116	TIGR00004, RutC_family_protein, reactive intermediate/imine deaminase. This protein was described initially as an inhibitor of protein synthesis intiation, then as an endoribonuclease active on single-stranded mRNA, endoribonuclease L-PSP. Members of this family, conserved in all domains of life and often with several members per bacterial genome, appear to catalyze a reaction that minimizes toxic by-products from reactions catalyzed by pyridoxal phosphate-dependent enzymes. [Cellular processes, Other].	2.44736e-69
NZ_CP020352.1\|WP_003178210.1\|64313_64544_+\|anti-sigma-F-factor-Fin-family-protein	gnl\|CDD\|371314	pfam10955, DUF2757, Protein of unknown function (DUF2757). Members in this family of proteins are annotated as YabK however currently no function is known.	8.40804e-42
NZ_CP020352.1\|WP_041817399.1\|47829_48705_+\|16S-rRNA-(cytidine(1402)-2'-O)-methyltransferase	gnl\|CDD\|223390	COG0313, COG0313, Predicted methyltransferases [General function prediction only].	1.06463e-135
NZ_CP020352.1\|WP_020449801.1\|52636_53833_+\|DUF348-domain-containing-protein	gnl\|CDD\|226111	COG3583, COG3583, Uncharacterized protein conserved in bacteria [Function unknown].	3.09343e-88
NZ_CP020352.1\|WP_020449800.1\|51624_52392_+\|TatD-family-hydrolase	gnl\|CDD\|223162	COG0084, TatD, Mg-dependent DNase [DNA replication, recombination, and repair].	8.52027e-134
NZ_CP020352.1\|WP_020449812.1\|64608_68142_+\|transcription-repair-coupling-factor	gnl\|CDD\|224118	COG1197, Mfd, Transcription-repair coupling factor (superfamily II helicase) [DNA replication, recombination, and repair / Transcription].	0
NZ_CP020352.1\|WP_020449803.1\|54553_55432_+\|16S-rRNA-(adenine(1518)-N(6)/adenine(1519)-N(6))--dimethyltransferase-RsmA	gnl\|CDD\|234708	PRK00274, ksgA, 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA.	1.39153e-140
NZ_CP020352.1\|WP_020449809.1\|61916_62867_+\|ribose-phosphate-diphosphokinase	gnl\|CDD\|234929	PRK01259, PRK01259, ribose-phosphate diphosphokinase.	0
NZ_CP020352.1\|WP_003178198.1\|59930_60224_+\|septation-protein-SpoVG	gnl\|CDD\|237321	PRK13259, PRK13259, septation regulator SpoVG.	4.50746e-59
NZ_CP020352.1\|WP_085959887.1\|48754_49045_-\|AbrB/MazE/SpoVT-family-DNA-binding-domain-containing-protein	gnl\|CDD\|224913	COG2002, AbrB, Regulators of stationary/sporulation gene expression [Transcription].	7.65552e-20
NZ_CP020352.1\|WP_020449806.1\|58521_59355_+\|pur-operon-repressor	gnl\|CDD\|236414	PRK09213, PRK09213, pur operon repressor; Provisional.	0
NZ_CP020352.1\|WP_020449799.1\|49552_51541_+\|methionine--tRNA-ligase	gnl\|CDD\|237028	PRK12267, PRK12267, methionyl-tRNA synthetase; Reviewed.	0
NZ_CP020352.1\|WP_003178187.1\|56697_56958_+\|hypothetical-protein	gnl\|CDD\|226872	COG4466, Veg, Uncharacterized protein conserved in bacteria [Function unknown].	6.55438e-39
NZ_CP020352.1\|WP_025809574.1\|57595_58465_+\|4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol-kinase	gnl\|CDD\|234652	PRK00128, ipk, 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase; Provisional.	0
NZ_CP020352.1\|WP_020449802.1\|54000_54561_+\|ribonuclease-M5	gnl\|CDD\|273019	TIGR00334, Ribonuclease_M5, ribonuclease M5. This family of orthologous proteins shows a weak but significant similarity to the central region of the DnaG-type DNA primase. The region of similarity is termed the Toprim (topoisomerase-primase) domain and is also shared by RecR, OLD family nucleases, and type IA and II topoisomerases. [Transcription, RNA processing].	2.76855e-74
NZ_CP020352.1\|WP_020449811.1\|63690_64257_+\|aminoacyl-tRNA-hydrolase	gnl\|CDD\|235457	PRK05426, PRK05426, peptidyl-tRNA hydrolase; Provisional.	1.45189e-100

>NZ_CP020352.1|WP_003178189.1|57114_57300_+|small,-acid-soluble-spore-protein,-alpha/beta-type
MGRRRSIMSEEFKNELAKDLGFYDTVKTEGWGGIRARDAGNMVKRAIELAEQHMAQNQQNH
>NZ_CP020352.1|WP_003178187.1|56697_56958_+|hypothetical-protein
MAKTLSDIKRSLDGHLGKRLTLKANGGRRKTIERSGILAETYPSVFVIQLDQDENSFERVSYSYADILTETVELRFSDDKTSSVAL
>NZ_CP020352.1|WP_023855980.1|55591_56473_+|sporulation-peptidase-YabG
MQFKIGDMVVRKSYRRDILFRIIRIDQSANGEPVAVLHGDEVRLIADAYLTDLEMVREAERQMRKREEESRMKESLDLLRQDYKLLHNKHEYRATNQYNNQQQYFHMPGRVLHLDGDSAYLKKCLALYEKIGVPVYGIHCYEKKMSSVIEELIDEYRPDLLVITGHDAYSKQKGDINNLDAYRHSKDFIETVQKARKKIPHLDQLVIFAGACQSHFESLIRAGANFASSPSRVNIHALDPVYIVAKISFTPFMDRINVWEVLRNTLTREKGLGGIETRGVLRIGMPYKTKEND
>NZ_CP020352.1|WP_020449803.1|54553_55432_+|16S-rRNA-(adenine(1518)-N(6)/adenine(1519)-N(6))--dimethyltransferase-RsmA
MNKDIATPIRTKEILNKYGFSFKKSLGQNFLIDTNILDRIVDHAGVTERTGVIEIGPGIGALTEQLAKRAKKVTAFEIDQRLLPILKDTLSPYDNVTVIHQDVLKADVRAVMDEQFQDCDEVMVVANLPYYVTTPIIMKLLEENLPLKGIVVMLQKEVADRMAAKPSTKEYGSLSIAVQFYTEAKTVMNVPKTVFVPQPNVDSAVIRLTLRGEPAVAVQDEAFFFQVVKSSFAQRRKTLFNNLVNNLPNGKENKSKIERALQDSQIDGKRRGESLSIEEFAVLSDRLRAVLL
>NZ_CP020352.1|WP_020449802.1|54000_54561_+|ribonuclease-M5
MKIKEIIVVEGRDDTAKIKSAVDADTIETNGSAIGEDVIKRIRLAQETRGVIILTDPDFPGEKIRKTIAEKVPGCKHAFLPKHLAKPKNKRGIGVEHASLEAIRECLKTVQEEMEATEPEIEARDLIEAGLIGGPLAKQRRERLGEILNIGYTNGKQLQKRLQMFQIKKSDYLSALDVVLREEEHE
>NZ_CP020352.1|WP_020449801.1|52636_53833_+|DUF348-domain-containing-protein
MKKLFSVKLSKSRWILIAAGLLVAGSGTAYGAHELAKQQVTISINGKKQDVRTNAETVGELLKSLDIETRKEDHISPAEDTKLTANTNVEYVAAKPVHFAVDGQEKEIWTTAGTVGKLLKDLNLELAEHDQIEPSVDKEITKDMKLTLQRAFKVTVKDAGKDKDIWTTSTTVADFLKHHKISIKGDDEVKPALDKKLTKDQAIHITRIKKVTDVVEENIPFEVRKKEDDSLEKGKEKVVRDGKHGKLKKHYAVVMKNGKEVSRELVKEETAAESKAKIIAVGTKQAAVATVSANVSRGNQSSGKEFYVSSTAYTASCNGCSGHTATGVNLKNNPGAKVIAVDPSVIPLGSKVHVEGYGYAVAADTGSAIKGRKIDVFFPSKSTAYRWGNKRVKIKVLN
>NZ_CP020352.1|WP_020449800.1|51624_52392_+|TatD-family-hydrolase
MLFDTHAHLNAEQFNEDLEEVIARAKEEKVEHIVVVGFDRPTIKRAMELIEAYDFIYAAIGWHPVDAIDMTDEDLAWIKDLSAHEKVVAIGEMGLDYHWDKSPKDVQKEVFRKQIALAKEVGLPIVIHNRDATEDVVSILREEGAEEVGGIMHCYTGSAEIAQECINMNFYLSFGGPVTFKNAKKPKEVVKQIPNDRLLIETDCPYLTPHPYRGKRNEPSYVKLVAEQIAELKGMTYEEIAAITTENAKKLFGIS
>NZ_CP020352.1|WP_020449799.1|49552_51541_+|methionine--tRNA-ligase
MPQEKNTFYITTPIYYPSGKLHIGHAYTTVAGDAMARYKRLRGFDVRYLTGTDEHGQKIQQTAEKENITPQELVDRAAEDIQQLWKKLDISNDDFIRTTEERHKNVIEKVFQKLLDNGDIYLDEYEGWYSIPDETFYTETQLVDVERNEKGEVIGGKSPDSGHPVELIKEESYFFRMGKYADRLLAFYEENPEFIQPESRKNEMINNFIKPGLEDLAVSRTTFDWGIKVPGNPKHVIYVWIDALFNYITALGFNTANDENYQKYWPADVHLVGKEIVRFHTIYWPIMLMALDLPLPKKVFAHGWLLMKDGKMSKSKGNVVDPVTLIDKYGLDALRYYLLREVPFGSDGVFTPEGFVERINYDLANDLGNLLNRTVAMVNKYFDGRVQSYEGPVTAFDEQLSSFSQKTIEAYEQAIENMEFSVALSSLWQFVSRTNKYIDETAPWVLAKDKDKEKDLQSVMYHLAESLRITAVLLQPFLTQTPEKIFAQLGVTDASLKTWDSIKSFGQLKSVKVQKGEPLFPRLEAEDEVAYIKSKMQGTAPKEEPKQEEKATERLPEITIDDFMSTELRVAEVIHAEPVKKADRLLKLQLDLGFEKRQVVSGIAKHYKPEELVGRKVICVTNLKPVKLRGELSQGMILAGEDNGVLSLAAVDSSLSNGTRIK
>NZ_CP020352.1|WP_085959887.1|48754_49045_-|AbrB/MazE/SpoVT-family-DNA-binding-domain-containing-protein
MNMKSTGIVRKVDELGRVVIPIELRRTLGIAEKDALEIYVDDEKIILKKYKPNMTCQVTGEVSDDNLKLAGGKLVLSPEGAEQIINEIQAQLQSQK
>NZ_CP020352.1|WP_041817399.1|47829_48705_+|16S-rRNA-(cytidine(1402)-2'-O)-methyltransferase
MINRQKSFDQKSDMGMLYLVPTPIGNLEDMTFRAVSVLKEADIIAAEDTRQTKKLCHVYEIETQLVSYHEHNKESSGHKILEWLKEGKNVALVSDAGMPTISDPGAEIVRDFIEMGGYVVPLPGANAALTALIASGIAPQPFFFFGFLDRNKRERKKQLEALKNRHETMIFYESPHRLKETLSAIREVFGNRQIALTRELTKKFEEFIRGSIEEALTWAEQEQVRGEFCLVIEGSREETIEESFWWEHLSEKEHVEHYISEGYHSKEAIKKTAVDRGVPKRKIYDAYHIQN
>NZ_CP020352.1|WP_025809574.1|57595_58465_+|4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol-kinase
MRILEKAPAKINLSLDVRRKRPDGYHEVEMVMTTIDLADRIELTELAEDKVTVSSHNRFVPDDQRNLAYQAAMLMKERYGIKKGVSIFITKVIPVAAGLAGGSSDAAAVFRGLNRLWDLKLSMNELAELGAEIGSDVSFCVHGGTALATGRGEKIRHIETPPHCWVVLAKPTIGVSTAEVYKQLKVDEIEHPDVQGMISAIEEKNFQKMCDKLGNVLESVTLNMHPEVAMIKNQMKRFGADAVLMSGSGPTVFGLVQYESKVQRIYNGLRGFCDQVYAVRMIGEQNELD
>NZ_CP020352.1|WP_020449806.1|58521_59355_+|pur-operon-repressor
MKFRRSGRLVDLTNYLLTHPHVLVPLTFFSERYQSAKSSISEDLTIIKQTFEQQGIGTLQTVPGAAGGVKYIPKVKRDEAEEFVKSLGESLIDSERILPGGYLYLTDILGKPSVLTKAGKIFASAFSDREIDVVMTVATKGIPLAYAVASYLDVPTVVVRKDNKVTEGSTVSINYVSGSSNRIQTMSLAKRNLQTGSRVLIIDDFMKAGGTINGMINLLEEFNAHVAGIGVLVEAQGVKERLVDEYMSLLTLSEIDMKEKKVEIQNGNFQRFFADNE
>NZ_CP020352.1|WP_020449807.1|59372_59750_+|RidA-family-protein
MTKVVQTKEAPAAIGPYSQGIVVNNMFYSSGQIPLTPSGEMVTGDITEQTHQVFRNLKAVLKEANASLETVVKATVFLADMNQFAEVNEVYGQYFDTHKPARSCVEVARLPKDALVEIEVVALVK
>NZ_CP020352.1|WP_003178198.1|59930_60224_+|septation-protein-SpoVG
MEVTDVRLRRVNTDGRMRAIASITLDHEFVVHDIRVIDGNNGLFVAMPSKRTPDGEFRDIAHPINSSTRGKIQDAVLNEYHRLGDVEEIEYEEAGAS
>NZ_CP020352.1|WP_020449808.1|60525_61896_+|bifunctional-UDP-N-acetylglucosamine-diphosphorylase/glucosamine-1-phosphate-N-acetyltransferase-GlmU
MDKRFAVVLAAGQGTRMKSKLYKVLHPVCGKPMVEHVVDEARKLSLEKLVTIVGHGAEDVKKHLGEKSEYALQAEQLGTAHAVKQAKSILGSEKGTTIVICGDTPLLTAETMEAMLNEHQKKAAKVTILTAVAEDPTGYGRVIRNENGDVAKIVEHKDATDEERLVKEINTGTYCFDNEALFQTIEQVSNDNVQGEYYLPDVIEILKNQGETVAAYQTGNFQETLGVNDRVALSQAEIYMKQRINKRHMGNGVSLIDPDNTYISPEAVIGRDTVIYPGTVIKGRVVIGEDAVIGQNSELENSTIGSRTVIKQSVVVNSEVGDDVTIGPFAHIRPDSKIGNEVRIGNFVEVKKSEFGDRSKASHLSYIGDAEIGTDVNLGCGSITVNYDGKHKFKTKIENGAFIGCNSNLVAPVTIGEGAYVAAGSTITDDVPGRALSIARARQVNKEDYAENIHKK
>NZ_CP020352.1|WP_020449809.1|61916_62867_+|ribose-phosphate-diphosphokinase
MSKYEDDNLKIFSLNSNPGLAKEIADIVGVELGKSSVKRFSDGEVQINIEESIRGCDCYVIQSTSAPVNEHIMELLIMVDALKRASAKTINIVMPYYGYARQDRKARSREPITAKLVANLLETAGATRVIALDLHAPQIQGFFDIPIDHLMGVPILGNYFEEKNFKDVVIVSPDHGGVTRARKLADRLKAPIAIIDKRRPKPNVAEVMNIIGHIEGKTAILIDDIIDTAGTITLAANALVESGAEEVYACCTHPVLSGPAIERIDNSKIKELVVTNSIELPEEKKIAKFKQLSVAPLLAEAIIRVHEKQSVSFLFD
>NZ_CP020352.1|WP_020449810.1|62947_63574_+|50S-ribosomal-protein-L25/general-stress-protein-Ctc
MAILKAEERTDSRRSSLRKIRQSGYVPGVIYGRNLENKSVALDSIELLKILRAEGKNTIINLDINGDQHSVMVTELQTDPLKDSIVHADFKVVDMEAEMEATVPVNLTGEAEGIKQGGVLQQPLYELSVTAKPKNIPQTIEVDISSLEVNDVLTVGDIPSKGDYSYNHEPDEVVASILPPQKQEETEAESAAQDVEEPEKGTEEEKEE
>NZ_CP020352.1|WP_020449811.1|63690_64257_+|aminoacyl-tRNA-hydrolase
MLVFAGLGNPGKTYENTRHNVGFMTIDELSKEWNIPLDKTKFNGQYGIGFVSGKKVLLVKPLTYMNLSGECLRPLLDYYEIPVDNLKVIYDDLDLPTGRIRLRTKGSAGGHNGIKSMIQHLGTTEFNRIRIGIGRPVNGMKIVDYVLGAFTDEEEPVIQEAVRQSAKACEASLEKPFLEVMNEFNAKV
>NZ_CP020352.1|WP_003178210.1|64313_64544_+|anti-sigma-F-factor-Fin-family-protein
MALHYYCRHCGVKVGSLDHSYVNSEQLGFNHLTNDERNDMISYMDNGDLHVKTICEDCQEALERNPDYHQYHSFIQ
>NZ_CP020352.1|WP_020449812.1|64608_68142_+|transcription-repair-coupling-factor
MNNIQSYITKSDDFKSIVNGLNEGLKEQLLAGLSGSARSLFTAALTKETRRPVFLITHNLYQAQKITDDLTGLIKDQPVLLYPVNELISSEIAVASPELRSQRLDVLNRLASGETPIVIAPAAAVRRMLPPVEVWQNSQIRIETGRDIDPEQLLQKLVQMGYERTDMVAAPGEFSIRGGIIDVYSLTEENPVRIELFDTEVDSIRIFNTDDQRSLETRDEVTIGPAKELIVRDEDRQRALEQIDKGLANSLKKLKLDKQKEILDQNIAEDKERLKEGHIGQEMVKYLSYFYERPASLLDYFPKNTLLILDEISRIHEMEDQLQKEEAEWITSLLEEGKILHDTAMSFPFHSLISKQSRPILYYSLFLRHVQHTSPQNIVNVSSKQMQSFHGQMNVLKNEIERFKKSKYTTVFLGDSKERVEKLASVLSDYEIEAAQADQDAALAQGQIYIMEGGLQSGFELPMIKLAVITEEELFKKRVKKQARKQKLTNAERIKSYSELQIGDYVVHVNHGIGKYLGIETLEINGIHKDYLNIHYQGSDKLYVPVDQIDQVQKYVGSEGKEPKLYKLGGSEWKRVKKKVESSVQDIADDLIKLYAEREASKGYAFSPDHEMQREFEAAFPYQETEDQLRSIHEIKRDMEKERPMDRLLCGDVGYGKTEVAIRAAFKAIADGKQVAILVPTTILAQQHYETILERFQDYPINIGLLSRFRTRKEANETIKGLKNGTIDIVIGTHRLLSKDVVYKDLGLLIIDEEQRFGVTHKEKIKRIKANVDVLTLTATPIPRTLHMSMLGVRDLSVIETPPENRFPVQTYVVEYNGALVREAIERELARGGQVYFLYNRVEDIERKAEEVSMLVPDAKVTYAHGKMTENELESVMLNFLEGESDVLVSTTIIETGVDIPNVNTLIVFDADKMGLSQLYQLRGRVGRSNRVAYAYFTYRKDKVLTEVAEKRLQAIKEFTELGSGFKIAMRDLTIRGAGNLLGAQQHGFIDSVGFDLYSQMLKEAIEARKGDVPQAEKFEPEIDLELDAYIPQTYVTDGKQKIDMYKRFRAISTIEEKSELQDEMIDRFGEYPKEVEYLFAIAEAKVYAITERVELIKQDKDAVRLTIDEKASAEIDGQKLFELGSKYGRQIGLGMEGKKLKISIQVKNRNPEEWLETLLEILKGLQNVKKQTIASS

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Crispr_ID: NZ_CP020352_2

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

NZ_CP020352_2

877976-879602

Orphan

Consensus_repeat	Method
CNACNGGNNCNACNGGNNCAACNGG	CRT

24 spacers

The CRISPR arrays of NZ_CP020352_2

>merge|NZ_CP020352|2|877976-879602|CRT
GAACAGGTCCAACCGGCCCGACAGGTCCAACAGGTCCAACCGGCCCGACTGGAGCAACCGGCCCAACAGGAGCAACCGGCCCGACCGGAGCAACCGGCCCAACAGGAGCAACCGGCCCGACGGGAGCAACCGGCCCAACAGGAGCAACCGGTCCGACGGGAGCAACCGGCCCAACAGGAGCAACCGGCCCGACGGGAGCAACCGGCCCAACAGGTCCAACCGGTCCGACGGGAGCAACCGGTCCGACGGGAGCAACCGGCCCAACAGGTCCAACAGGCGCAACCGGAGCAACAGGCGCAACGGGAGCGACGGGAGCAACCGGCCCAACAGGAGCAACCGGCCCGACGGGAGCAACCGGCCCAACAGGTCCAACAGGCGCAACCGGAGCAACAGGCGCAACGGGAGCGACGGGAGCAACCGGTCCGACGGGAGCAACGGGAGCGACAGGCGCAACCGGAGCAACCGGCCCGACGGGAGCAACCGGCCCGACGGGAGCAACGGGAGCGACAGGCGCAACCGGAGCAACCGGCCCGACAGGCCCAACCGGAGCGACAGGAGCAACCGGTCCGACGGGAGCAACCGGCCCGACAGGAGCAACGGGAGCGACAGGCGCAACAGGTCCGACGGGAGCAACGGGAGCGACAGGCGCAACAGGTCCGACGGGAGCAACCGGCCCAACAGGTCCAACAGGCGCAACCGGAGCGACAGGCGCAACAGGTCCGACGGGAGCAACCGGAGCGACAGGCGCAACCGGAGCGACAGGAGCAACCGGTCCGACAGGAGCAACCGGAGCGACAGGCGCAACCGGAGCGACAGGCGCAACGGGAGCGACAGGCGCAACGGGAGCGACAGGCGCAACGGGAGCGACAGGCGCAACGGGAGCGACAGGAGCAACCGGTCCGACGGGAGCAACGGGAGCGACAGGAGCAACCGGCCCAACAGGTCCGACGGGAGCAACGGGAGCGACAGGAGCAACCGGCCCAACGGGAGCAACGGGAGCGACAGGAGCAACCGGTCCGACGGGAGCAACCGGCCCGACAGGAGCAACGGGAGCGACAGGAGCAACGGGAGCGACAGGAGCAACAGGAGCGACAGGAGCAACCGGCCCGACAGGAGCAACAGGTCCAACAGGAGCAACAGGAGCGACAGGCCCAACAGGTCCGACGGGAGCAACTGGCCCGACAGGAGCAACCGGGGCAACCGGTCCGACAGGAGCAACCGGTCCGACAGGCCCAACCGGAGCAACGGGAGCAACCGGCCCAACGGGAGCAACCGGCCCGACGGGAGCAACAGGTCCGACGGGAGCAACCGGGGCAACCGGAGCAACGGGAGCAACCGGCCCGACGGGAGCAACCGGTCCAACCGGAGCAACGGGAGCGACAGGCGCAACCGGTCCAACAGGTCCAACCGGAGCGACAGGTCCAACCGGCCCAACAGGTCCGACGGGAGCAACCGGTCCAACCGGAGCAACGGGAGCGACAGGTCCAACAGGTCCAACCGGAGCGACAGGTCCAACTGGCCCGACAGGTCCAACTGGCCCGACAGGTCCAACTGGCCCGACAGGTCCAACTGGCCCGACGGGTCCAACTGGCCCGACAGGTCCAACTGGCCCGACAGGTCCAACCGG

>NZ_CP020352|2|1|877976-879602|CRT
GAACAGGTCCAACCGGCCCGACAGG	TCCAACAGGTCCAACCGGCC
CGACTGGAGCAACCGGCCCAACAGG	AGCAACCGGCCCGACCGGAGCAACCGGCCCAACAGGAGCAACCGGCC
CGACGGGAGCAACCGGCCCAACAGG	AGCAACCGGTCCGACGGGAGCAACCGGCCCAACAGGAGCAACCGGCC
CGACGGGAGCAACCGGCCCAACAGG	TCCAACCGGTCCGACGGGAGCAACCGGTC
CGACGGGAGCAACCGGCCCAACAGG	TCCAACAGGCGCAACCGGAGCAACAGGCGCAACGGGAG
CGACGGGAGCAACCGGCCCAACAGG	AGCAACCGGCCCGACGGGAG
CAACCGGCCCAACAGGTCCAACAGG	CGCAACCGGAGCAACAGGCGCAACGGGAGCGACGGGAG
CAACCGGTCCGACGGGAGCAACGGG	AGCGACAGGCGCAACCGGAGCAACCGGCCCGACGGGAG
CAACCGGCCCGACGGGAGCAACGGG	AGCGACAGGCGCAACCGGAGCAACCGGCCCGACAGGCCCAACCGGAGCGACAGGAG
CAACCGGTCCGACGGGAGCAACCGG	CCCGACAGGAGCAACGGGAGCGACAGGCGCAACAGGTCCGACGGGAGCAACGGGAGCGACAGGCG
CAACAGGTCCGACGGGAGCAACCGG	CCCAACAGGTCCAACAGGCGCAACCGGAGCGACAGGCG
CAACAGGTCCGACGGGAGCAACCGG	AGCGACAGGCGCAACCGGAGCGACAGGAG
CAACCGGTCCGACAGGAGCAACCGG	AGCGACAGGCGCAACCGGAGCGACAGGCG
CAACGGGAGCGACAGGCGCAACGGG	AGCGACAGGCGCAACGGGAGCGACAGGCG
CAACGGGAGCGACAGGAGCAACCGG	TCCGACGGGAGCAACGGGAGCGACAGGAGCAACCGGCCCAACAGGTCCGACGGGAG
CAACGGGAGCGACAGGAGCAACCGG	CCCAACGGGAGCAACGGGAGCGACAGGAGCAACCGGTCCGACGGGAGCAACCGGCCCGACAGGAG
CAACGGGAGCGACAGGAGCAACGGG	AGCGACAGGAGCAACAGGAGCGACAGGAGCAACCGGCCCGACAGGAGCAACAGGTC
CAACAGGAGCAACAGGAGCGACAGG	CCCAACAGGTCCGACGGGAGCAACTGGCCCGACAGGAGCAACCGGGGCAACCGGTC
CGACAGGAGCAACCGGTCCGACAGG	CCCAACCGGAGCAACGGGAGCAACCGGCCCAACGGGAGCAACCGGCC
CGACGGGAGCAACAGGTCCGACGGG	AGCAACCGGGGCAACCGGAGCAACGGGAGCAACCGGCC
CGACGGGAGCAACCGGTCCAACCGG	AGCAACGGGAGCGACAGGCGCAACCGGTCCAACAGGTC
CAACCGGAGCGACAGGTCCAACCGG	CCCAACAGGTCCGACGGGAGCAACCGGTCCAACCGGAGCAACGGGAGCGACAGGTCCAACAGGTC
CAACCGGAGCGACAGGTCCAACTGG	CCCGACAGGTCCAACTGGCCCGACAGGTC
CAACTGGCCCGACAGGTCCAACTGG	CCCGACGGGTCCAACTGGCCCGACAGGTC
CAACTGGCCCGACAGGTCCAACCGG

Protein	Signature genes	Signature genes Name	Protein_function
NZ_CP020352.1\|WP_025810396.1\|868915_869608_+\|N-acetyltransferase	unknown	unknown	gnl\|CDD\|100048
NZ_CP020352.1\|WP_080131270.1\|864896_866309_+\|PTS-system-trehalose-specific-EIIBC-component	unknown	unknown	gnl\|CDD\|273916
NZ_CP020352.1\|WP_080131278.1\|889915_891211_+\|nucleotide-sugar-dehydrogenase	unknown	unknown	gnl\|CDD\|274399
NZ_CP020352.1\|WP_080131274.1\|881450_882425_-\|NAD-dependent-epimerase/dehydratase-family-protein	unknown	unknown	gnl\|CDD\|187566
NZ_CP020352.1\|WP_080131271.1\|866382_868071_+\|alpha,alpha-phosphotrehalase	unknown	unknown	gnl\|CDD\|274115
NZ_CP020352.1\|WP_080131559.1\|871564_877261_+\|NTTRR-F1-domain	unknown	unknown	gnl\|CDD\|223021
NZ_CP020352.1\|WP_080131279.1\|891291_892203_+\|alpha/beta-hydrolase	unknown	unknown	gnl\|CDD\|223999
NZ_CP020352.1\|WP_023855858.1\|884018_886262_-\|glycosyltransferase	unknown	unknown	gnl\|CDD\|133055
NZ_CP020352.1\|WP_020450556.1\|886455_887538_+\|glycosyltransferase	unknown	unknown	gnl\|CDD\|133005
NZ_CP020352.1\|WP_020450546.1\|868092_868809_+\|trehalose-operon-repressor	unknown	unknown	gnl\|CDD\|274116
NZ_CP020352.1\|WP_080131276.1\|887659_888769_+\|DegT/DnrJ/EryC1/StrS-family-aminotransferase	unknown	unknown	gnl\|CDD\|99740
NZ_CP020352.1\|WP_080131272.1\|869734_871339_+\|collagen-like-protein	unknown	unknown	gnl\|CDD\|236090
NZ_CP020352.1\|WP_080131277.1\|888765_889830_+\|Gfo/Idh/MocA-family-oxidoreductase	unknown	unknown	gnl\|CDD\|223745
NZ_CP020352.1\|WP_026580665.1\|880890_881433_-\|transferase	unknown	unknown	gnl\|CDD\|100047
NZ_CP020352.1\|WP_080131273.1\|879802_880894_-\|DegT/DnrJ/EryC1/StrS-family-aminotransferase	unknown	unknown	gnl\|CDD\|99740
NZ_CP020352.1\|WP_080131269.1\|863295_864708_+\|sodium:alanine-symporter-family-protein	unknown	unknown	gnl\|CDD\|224040
NZ_CP020352.1\|WP_020450542.1\|862847_863225_+\|DUF1992-domain-containing-protein	unknown	unknown	gnl\|CDD\|378159
NZ_CP020352.1\|WP_080131268.1\|860623_862585_-\|LTA-synthase-family-protein	unknown	unknown	gnl\|CDD\|224287
NZ_CP020352.1\|WP_080131275.1\|882491_883865_-\|glycosyltransferase	unknown	unknown	gnl\|CDD\|223515
NZ_CP020352.1\|WP_035336955.1\|859745_860498_-\|type-I-methionyl-aminopeptidase	unknown	unknown	gnl\|CDD\|237252

Protein	Function_ID	Function_description	E-value
NZ_CP020352.1\|WP_025810396.1\|868915_869608_+\|N-acetyltransferase	gnl\|CDD\|100048	cd03358, LbH_WxcM_N_like, WcxM-like, Left-handed parallel beta-Helix (LbH) N-terminal domain: This group is composed of Xanthomonas campestris WcxM and proteins with similarity to the WcxM N-terminal domain. WcxM is thought to be bifunctional, catalyzing both the isomerization and transacetylation reactions of keto-hexoses. It contains an N-terminal LbH domain responsible for the transacetylation function and a C-terminal isomerase domain. The LbH domain contains imperfect tandem repeats of a hexapeptide repeat motif (X-[STAV]-X-[LIV]-[GAED]-X), typical of enzymes with acyltransferase activity.	2.3885e-50
NZ_CP020352.1\|WP_080131270.1\|864896_866309_+\|PTS-system-trehalose-specific-EIIBC-component	gnl\|CDD\|273916	TIGR01992, phosphotransferase_system_trehalose_permease, PTS system, trehalose-specific IIBC component. This model represents the fused enzyme II B and C components of the trehalose-specific PTS sugar transporter system. Trehalose is converted to trehalose-6-phosphate in the process of translocation into the cell. These transporters lack their own IIA domains and instead use the glucose IIA protein (IIAglc or Crr). The exceptions to this rule are Staphylococci and Streptococci which contain their own A domain as a C-terminal fusion. This family is closely related to the sucrose transporting PTS IIBC enzymes and the B and C domains of each are described by subfamily-domain level TIGRFAMs models (TIGR00826 and TIGR00852, respectively). In E. coli, B. subtilis and P. fluorescens the presence of this gene is associated with the presence of trehalase which degrades T6P to glucose and glucose-6-P. Trehalose may also be transported (in Salmonella) via the mannose PTS or galactose permease systems, or (in Sinorhizobium, Thermococcus and Sulfolobus, for instance) by ABC transporters.	0
NZ_CP020352.1\|WP_080131278.1\|889915_891211_+\|nucleotide-sugar-dehydrogenase	gnl\|CDD\|274399	TIGR03026, NDP-sugDHase, nucleotide sugar dehydrogenase. Enzymes in this family catalyze the NAD-dependent alcohol-to-acid oxidation of nucleotide-linked sugars. Examples include UDP-glucose 6-dehydrogenase (1.1.1.22), GDP-mannose 6-dehydrogenase (1.1.1.132), UDP-N-acetylglucosamine 6-dehydrogenase (1.1.1.136), UDP-N-acetyl-D-galactosaminuronic acid dehydrogenase, and UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase. These enzymes are most often involved in the biosynthesis of polysaccharides and are often found in operons devoted to that purpose. All of these enzymes contain three Pfam domains, pfam03721, pfam00984, and pfam03720 for the N-terminal, central, and C-terminal regions respectively.	1.07435e-179
NZ_CP020352.1\|WP_080131274.1\|881450_882425_-\|NAD-dependent-epimerase/dehydratase-family-protein	gnl\|CDD\|187566	cd05256, UDP_AE_SDR_e, UDP-N-acetylglucosamine 4-epimerase, extended (e) SDRs. This subgroup contains UDP-N-acetylglucosamine 4-epimerase of Pseudomonas aeruginosa, WbpP, an extended SDR, that catalyzes the NAD+ dependent conversion of UDP-GlcNAc and UDPGalNA to UDP-Glc and UDP-Gal. This subgroup has the characteristic active site tetrad and NAD-binding motif of the extended SDRs. Extended SDRs are distinct from classical SDRs. In addition to the Rossmann fold (alpha/beta folding pattern with a central beta-sheet) core region typical of all SDRs, extended SDRs have a less conserved C-terminal extension of approximately 100 amino acids. Extended SDRs are a diverse collection of proteins, and include isomerases, epimerases, oxidoreductases, and lyases; they typically have a TGXXGXXG cofactor binding motif. SDRs are a functionally diverse family of oxidoreductases that have a single domain with a structurally conserved Rossmann fold, an NAD(P)(H)-binding region, and a structurally diverse C-terminal region. Sequence identity between different SDR enzymes is typically in the 15-30% range; they catalyze a wide range of activities including the metabolism of steroids, cofactors, carbohydrates, lipids, aromatic compounds, and amino acids, and act in redox sensing. Classical SDRs have an TGXXX[AG]XG cofactor binding motif and a YXXXK active site motif, with the Tyr residue of the active site motif serving as a critical catalytic residue (Tyr-151, human 15-hydroxyprostaglandin dehydrogenase numbering). In addition to the Tyr and Lys, there is often an upstream Ser and/or an Asn, contributing to the active site; while substrate binding is in the C-terminal region, which determines specificity. The standard reaction mechanism is a 4-pro-S hydride transfer and proton relay involving the conserved Tyr and Lys, a water molecule stabilized by Asn, and nicotinamide. Atypical SDRs generally lack the catalytic residues characteristic of the SDRs, and their glycine-rich NAD(P)-binding motif is often different from the forms normally seen in classical or extended SDRs. Complex (multidomain) SDRs such as ketoreductase domains of fatty acid synthase have a GGXGXXG NAD(P)-binding motif and an altered active site motif (YXXXN). Fungal type ketoacyl reductases have a TGXXXGX(1-2)G NAD(P)-binding motif.	1.06637e-69
NZ_CP020352.1\|WP_080131271.1\|866382_868071_+\|alpha,alpha-phosphotrehalase	gnl\|CDD\|274115	TIGR02403, Trehalose-6-phosphate_hydrolase, alpha,alpha-phosphotrehalase. Trehalose is a glucose disaccharide that serves in many biological systems as a compatible solute for protection against hyperosmotic and thermal stress. This family describes trehalose-6-phosphate hydrolase, product of the treC (or treA) gene, which is often found together with a trehalose uptake transporter and a trehalose operon repressor.	0
NZ_CP020352.1\|WP_080131559.1\|871564_877261_+\|NTTRR-F1-domain	gnl\|CDD\|223021	PHA03247, PHA03247, large tegument protein UL36; Provisional.	2.33156e-06
NZ_CP020352.1\|WP_080131279.1\|891291_892203_+\|alpha/beta-hydrolase	gnl\|CDD\|223999	COG1073, COG1073, Hydrolases of the alpha/beta superfamily [General function prediction only].	7.05595e-17
NZ_CP020352.1\|WP_023855858.1\|884018_886262_-\|glycosyltransferase	gnl\|CDD\|133055	cd06433, GT_2_WfgS_like, WfgS and WfeV are involved in O-antigen biosynthesis. Escherichia coli WfgS and Shigella dysenteriae WfeV are glycosyltransferase 2 family enzymes involved in O-antigen biosynthesis. GT-2 enzymes have GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. These are enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. Glycosyltransferases have been classified into more than 90 distinct sequence based families.	1.86082e-23
NZ_CP020352.1\|WP_020450556.1\|886455_887538_+\|glycosyltransferase	gnl\|CDD\|133005	cd02511, Beta4Glucosyltransferase, UDP-glucose LOS-beta-1,4 glucosyltransferase is required for biosynthesis of lipooligosaccharide. UDP-glucose: lipooligosaccharide (LOS) beta-1-4-glucosyltransferase catalyzes the addition of the first residue, glucose, of the lacto-N-neotetrase structure to HepI of the LOS inner core. LOS is the major constituent of the outer leaflet of the outer membrane of gram-positive bacteria. It consists of a short oligosaccharide chain of variable composition (alpha chain) attached to a branched inner core which is lined in turn to lipid A. Beta 1,4 glucosyltransferase is required to attach the alpha chain to the inner core.	1.16222e-45
NZ_CP020352.1\|WP_020450546.1\|868092_868809_+\|trehalose-operon-repressor	gnl\|CDD\|274116	TIGR02404, Trehalose_operon_transcriptional_repressor, trehalose operon repressor, B. subtilis-type. This family consists of repressors of the GntR family typically associated with trehalose utilization operons. Trehalose is imported as trehalose-6-phosphate and then hydrolyzed by alpha,alpha-phosphotrehalase to glucose and glucose-6-P. This family includes repressors mostly from Gram-positive lineages and does not include the TreR from E. coli. [Regulatory functions, DNA interactions].	2.87712e-117
NZ_CP020352.1\|WP_080131276.1\|887659_888769_+\|DegT/DnrJ/EryC1/StrS-family-aminotransferase	gnl\|CDD\|99740	cd00616, AHBA_syn, 3-amino-5-hydroxybenzoic acid synthase family (AHBA_syn). AHBA_syn family belongs to pyridoxal phosphate (PLP)-dependent aspartate aminotransferase superfamily (fold I). The members of this CD are involved in various biosynthetic pathways for secondary metabolites. Some well studied proteins in this CD are AHBA_synthase, protein product of pleiotropic regulatory gene degT, Arnb aminotransferase and pilin glycosylation protein. The prototype of this family, the AHBA_synthase, is a dimeric PLP dependent enzyme. AHBA_syn is the terminal enzyme of 3-amino-5-hydroxybenzoic acid (AHBA) formation which is involved in the biosynthesis of ansamycin antibiotics, including rifamycin B. Some members of this CD are involved in 4-amino-6-deoxy-monosaccharide D-perosamine synthesis. Perosamine is an important element in the glycosylation of several cell products, such as antibiotics and lipopolysaccharides of gram-positive and gram-negative bacteria. The pilin glycosylation protein encoded by gene pglA, is a galactosyltransferase involved in pilin glycosylation. Additionally, this CD consists of ArnB (PmrH) aminotransferase, a 4-amino-4-deoxy-L-arabinose lipopolysaccharide-modifying enzyme. This CD also consists of several predicted pyridoxal phosphate-dependent enzymes apparently involved in regulation of cell wall biogenesis. The catalytic lysine which is present in all characterized PLP dependent enzymes is replaced by histidine in some members of this CD.	2.84219e-171
NZ_CP020352.1\|WP_080131272.1\|869734_871339_+\|collagen-like-protein	gnl\|CDD\|236090	PRK07764, PRK07764, DNA polymerase III subunits gamma and tau; Validated.	2.14569e-09
NZ_CP020352.1\|WP_080131277.1\|888765_889830_+\|Gfo/Idh/MocA-family-oxidoreductase	gnl\|CDD\|223745	COG0673, MviM, Predicted dehydrogenases and related proteins [General function prediction only].	1.47367e-70
NZ_CP020352.1\|WP_026580665.1\|880890_881433_-\|transferase	gnl\|CDD\|100047	cd03357, LbH_MAT_GAT, Maltose O-acetyltransferase (MAT) and Galactoside O-acetyltransferase (GAT): MAT and GAT catalyze the CoA-dependent acetylation of the 6-hydroxyl group of their respective sugar substrates. MAT acetylates maltose and glucose exclusively at the C6 position of the nonreducing end glucosyl moiety. GAT specifically acetylates galactopyranosides. Furthermore, MAT shows higher affinity toward artificial substrates containing an alkyl or hydrophobic chain as well as a glucosyl unit. Active MAT and GAT are homotrimers, with each subunit consisting of an N-terminal alpha-helical region and a C-terminal left-handed parallel alpha-helix (LbH) subdomain with 6 turns, each containing three imperfect tandem repeats of a hexapeptide repeat motif (X-[STAV]-X-[LIV]-[GAED]-X).	1.10558e-36
NZ_CP020352.1\|WP_080131273.1\|879802_880894_-\|DegT/DnrJ/EryC1/StrS-family-aminotransferase	gnl\|CDD\|99740	cd00616, AHBA_syn, 3-amino-5-hydroxybenzoic acid synthase family (AHBA_syn). AHBA_syn family belongs to pyridoxal phosphate (PLP)-dependent aspartate aminotransferase superfamily (fold I). The members of this CD are involved in various biosynthetic pathways for secondary metabolites. Some well studied proteins in this CD are AHBA_synthase, protein product of pleiotropic regulatory gene degT, Arnb aminotransferase and pilin glycosylation protein. The prototype of this family, the AHBA_synthase, is a dimeric PLP dependent enzyme. AHBA_syn is the terminal enzyme of 3-amino-5-hydroxybenzoic acid (AHBA) formation which is involved in the biosynthesis of ansamycin antibiotics, including rifamycin B. Some members of this CD are involved in 4-amino-6-deoxy-monosaccharide D-perosamine synthesis. Perosamine is an important element in the glycosylation of several cell products, such as antibiotics and lipopolysaccharides of gram-positive and gram-negative bacteria. The pilin glycosylation protein encoded by gene pglA, is a galactosyltransferase involved in pilin glycosylation. Additionally, this CD consists of ArnB (PmrH) aminotransferase, a 4-amino-4-deoxy-L-arabinose lipopolysaccharide-modifying enzyme. This CD also consists of several predicted pyridoxal phosphate-dependent enzymes apparently involved in regulation of cell wall biogenesis. The catalytic lysine which is present in all characterized PLP dependent enzymes is replaced by histidine in some members of this CD.	6.02545e-107
NZ_CP020352.1\|WP_080131269.1\|863295_864708_+\|sodium:alanine-symporter-family-protein	gnl\|CDD\|224040	COG1115, AlsT, Na+/alanine symporter [Amino acid transport and metabolism].	0
NZ_CP020352.1\|WP_020450542.1\|862847_863225_+\|DUF1992-domain-containing-protein	gnl\|CDD\|378159	pfam09350, DUF1992, Domain of unknown function (DUF1992). This family of proteins are functionally uncharacterized.	9.13129e-19
NZ_CP020352.1\|WP_080131268.1\|860623_862585_-\|LTA-synthase-family-protein	gnl\|CDD\|224287	COG1368, MdoB, Phosphoglycerol transferase and related proteins, alkaline phosphatase superfamily [Cell envelope biogenesis, outer membrane].	0
NZ_CP020352.1\|WP_080131275.1\|882491_883865_-\|glycosyltransferase	gnl\|CDD\|223515	COG0438, RfaG, Glycosyltransferase [Cell envelope biogenesis, outer membrane].	1.60392e-16
NZ_CP020352.1\|WP_035336955.1\|859745_860498_-\|type-I-methionyl-aminopeptidase	gnl\|CDD\|237252	PRK12896, PRK12896, methionine aminopeptidase; Reviewed.	1.68788e-142

>NZ_CP020352.1|WP_080131559.1|871564_877261_+|NTTRR-F1-domain
MENRIINGGFETGSLPPWTGFNAVVTSNFSHSGIYSVNLVTSSIGILAQSFLVNPGENFEFVISISKASSAPSPAIVITVDYYNSSFDFLSQGLSLTIPVNRLSAVNVWNEIYATTNSAPPGTVIGVVTIQKLPLSGGAPILIDDVAMITIDQPGATGPTGATGPTGATGPTGATGATGPTGATGATGPTGATGPTGATGATGPTGPTGATGPTGPTGATGPTGPTGATGATGPTGATGSTGATGPTGATGPTGATGATGVTGPTGSTGPTGVTGPTGATGPTGPTGATGPTGPTGATGATGPTGATGSTGATGPTGATGPTGATGATGVTGPTGSTGPTGVTGPTGVTGPTGVTGPTGATGPTGATGATGAMGPTGATGPTGPTGATGPTGVTGATGATGATGATGPTGATGATGPTGSTGPTGATGATGPTGSTGPTGATGATGATGSTGPTGVTGATGATGVTGPTGPTGVTGATGATGPTGATGATGPTGSTGPTGATGPTGATGPTGSTGATGPTGATGPTGATGATGATGATGPTGSTGATGPTGPTGATGATGATGATGATGPTGATGPTGPTGATGPTGVTGATGATGPTGATGATGATGPTGATGATGATGPTGSTGATGPTGPTGATGATGATGATGVTGPTGVTGPTGATGATGATGPTGATGPTGPTGATGPTGATGATGPTGATGPTGATGATGAIGATGPTGSTGATGPTGPTGATGATGATGATGATGATGPTGATGPTGPTGATGPTGVTGATGATGPTGATGATGATGPTGATGATGATGPTGSTGATGPTGPTGATGATGATGATGVTGPTGVTGPTGATGATGATGPTGATGPTGPTGATGPTGVTGPTGATGPTGSTGPTGATGATGATGSTGPTGATGPTGVTGATGATGVTGPTGPTGVTGATGATGPTGATGATGATGPTGTTGPTGATGPTGATGPTGATGATGPTGATGPTGATGATGATGATGPTGSTGATGPTGPTGATGATGATGATGATGATGPTGATGPTGPTGATGATGATGATGPTGPTGATGATGATGATGVTGPTGVTGPTGATGATGATGPTGATGPTGPTGATGPTGATGPTGPTGSTGPTGATGATGATGSTGPTGATGPTGVTGATGATGVTGPTGPTGVTGATGATGPTGATGATGATGPTGTTGPTGATGPTGATGPTGATGATGPTGATGPTGATGATGATGSTGPTGATGPIGATGATGPTGATGATGPTGATGATGPTGATGPTGPTGATGATGATGATGATGATGPTGATGPTGATGATGPTGATGPTGATGATGATGSTGPTGATGPIGATGATGPTGPTGATGSTGPTGATGATGATGPTGATGPTGATGPTGPTGVTGATGATGPTGATGPTGATGATGPTGSTGATGPTGPTGATGATGATGATGATGPTGATGPTGPTGVTGATGATGPTGATGPTGVTGATGATGPTGPTGATGATGPTGAAGPTGPTGATGATGPTGAAGPTGATGPTGATGPTGATGPTGATGPTGVTGPTGATGPTGVTGATGATGATGATGATGPTGATGATGPTGATGPTGVTGATGATGPTGATGATGPTGATGPTGVTGATGATGATGATGATGATGPTGATGPTGPTGATGPTGVTGATGATGPTGATGATGPTGATGPTGVTGATGATGPTGATGPTGPTGATGPTGVTGATGATGPTGATGATGPTGATGPTGPQGPTGPPGEVGVMVVAGPTGPTGPASPQTAIFFNNLFGSTTVYNPPTIPLNTEVTVVSVPNVSVTSGQNIKVDYALAIEAVNTANSNIIFEIRLYRGGTLVQTRIFNRSLAAAGTQRFPLASTIVDTIPATATVEYSVRVAVTMASNVTSAAAFNRNLNLIRFP
>NZ_CP020352.1|WP_080131272.1|869734_871339_+|collagen-like-protein
MSIQNAVMNGGFETGAFSPWVGINASVTSQFSHSGFFSARFLGGTATSYIAQFVPARAGEGSEFLVSLAKAGFLPAPSITIQVTYFDSQFNFLEYGLFATVPSSRIPAAENGTWLEVYQTTSPAPPGTTQAFILINNIPQAGTASVLVDDVALLSVEGGGGPTGATGPTGATGPTGPTGPTGATGATGPTGSTGPTGATGSTGPTGSTGPTGSTGPTGATGPTGSTGPTGATGATGPTGSTGPTGATGPTGATGPTGVTGPTGSTGATGSTGPTGATGSTGPTGATGATGPTGVTGPTGATGATGSTGPTGATGSTGATGPTGATGATGATGPTGVTGPTGATGPTGATGATGATGPTGATGPTGATGPTGPTGATGPTGVTGPTGATGPTGATGPTGPGSLLGTPAQSTDPVSLTVGSETPILTTTVTSSAGQNIKLDSMAEVDIIIGPATTFEYSIIFTLYRNDVAIAQVSVDSQDEKSAGSVRVYGDIPNLTWVDTPGAGTQTYEVRINVTGTNLLAANVNTRALNAVIFD
>NZ_CP020352.1|WP_025810396.1|868915_869608_+|N-acetyltransferase
MIHETVKIGKNVVLGEHAVIEENVVIGDNVTIGHHAIIKKDTQIGSGVKIGDLAVLGKAASSNKKMARQPKQAGAPLRIEDDAVVGASAVIYRDVLLEKGVFVGDMASIRENVAIGRESIIGRNAMVENNTKIGKRVTIQTGCYITADMTIEDEVFIGPCCSTSNDKYMGMGNYPYQGPTIKRGAKIGNNATLLPAVVIGEHAVIGAGAVITKDVPAGKTAVGNPGRLLK
>NZ_CP020352.1|WP_020450546.1|868092_868809_+|trehalose-operon-repressor
MKINKYMMIYKQIAEQIDQSILNAGDILPSENDLAEQHDTSRETVRKALNVLAQNGYIQKIKGKGSVVLHREKMQFPVSGLVSFKELSETLGKETSTTVHEFGLTHPDAYIQKQLRTSGDEEVWRVVRSRNINGERIILDKDYFIRKHVPLLTKEICEDSIYQYLEGECGLVISYAHKEIVVEPCTAEDQEYLDLNGYEHIVVVKNFVFLDDTTLFQYTESRHRLDKFRFVDFARREK
>NZ_CP020352.1|WP_080131271.1|866382_868071_+|alpha,alpha-phosphotrehalase
METKENPWWKKAVVYQIYPKSFKDTTGNGVGDIRGIIEKLDYIKELACDVIWLTPIYQSPQNDNGYDISDYYNIHEEYGTMADFEELLEEAHKRGIKVIMDLVVNHTSTEHKWFKEAASGKENPYRDFYIWKDMKPDGAPPTNWESKFGGSAWEFHEETGQYYLHLYDVTQADLNWENDEVRKKVYKMMHFWFEKGIDGFRLDVINVISKDQRFPDDDQGDGRRFYTDGPRVHEFLNEMNREVFSKYDSMTVGEMSSTTIADCIRYTNPESRELDMVFNFHHLKADYPNGEKWALADFDFLKLKKILSEWQTEMHKGGGWNALFWCNHDQPRIVSRYGDDGKYRIKSAKMLATAIHMLQGTPYIYQGEELGMTNPKFDDISLYRDVESLNMYRLLKEAGKPEAEIIEILKAKSRDNSRTPVQWNGEKNAGFTTGTPWIPVPDNYKEINAEKALSDPDSIFYHYKKLNKLRKEFDIITTGDYQLILEDDQALYAYLRNGADEKLLVINNFYGKETEFQLPADIDIEGYDAKVLISNDTDLPESFTRFKVKPYQSIVYHLAKPC
>NZ_CP020352.1|WP_080131270.1|864896_866309_+|PTS-system-trehalose-specific-EIIBC-component
MTDLNQSARRIVDAVGGADNIAAVTHCVTRLRFALLDESKVNQEVLEQIDVVKGSFSTNGQFQVVIGQGTVNNVYAELVKATGIGESSKDDVKKASEKNMNPLQRAVKTLADIFIPILPAIVTAGLLMGINNILTAQDIFFSGKSIVEVYPQWADLANMINLIAGTAFAFLPALIGWSAVKRFGGNPLLGIVLGVMLVHPDLLNAWGYGAAEQSGEIPVWNLFGLEVQKVGYQGQVLPILLASYLLAKIERFLTKRTPESIQLLVVAPITLLVTGFASFIVIGPITFAIGNVLTSGLIAVFEQFAVLGGLLYGGLYAALVITGMHHTFLAVDLQLIGSKLGGTFLWPMLALSNIAQGSAALAMMFIVKDEKQKGLSLTSGISAYLGITEPALFGVNLRYRFPFVIAMISSGIAGMFISSQGVLASSVGVGGVPGIFSIMSQFWGAFAVGMAIVLIAPFAGTYLYAKLKMK
>NZ_CP020352.1|WP_080131269.1|863295_864708_+|sodium:alanine-symporter-family-protein
MERLLNWVEYISDWLWGPPLIILLTGTGVYLTILLKFFQFRYPLYIFKQTIGSVGKKPKGEGTVTPLQALTSALSSTVGAANIVGVPAAIMFGGPGAVFWMWLVALFAMAIKFSESVLAVHYREKNEEGEYVGGPMYYITKGLSMKWLGVFFSVALMIELVPSIMVQGNSVSASLAESFSFNRLYTGIGIAFLIGLVVIGGIKRIGKVTELAVPLMAGAYIGAGFLIVVMNLSSVPAFFSLIFSNAFTSSSAFGGFAGAAVAETVRWGFARGLYSNEAGMGTAPIAHAAAMTDHPVRQGFWAVIGIVVDTLLICTTTAFVVLASGVWTDENAASNPGALAAAAFRSYFGPVGGYFVSVSLVFFVLSTITVVIFYGVKQAEFLFGRLAGHMIKFVYLAAVIIGAAGGAKAIWGFLDLALVFILIPNILAVLLLSKKVRSLYDEFFTSEKYYLKDIGQQDGADERDEKVNNL
>NZ_CP020352.1|WP_020450542.1|862847_863225_+|DUF1992-domain-containing-protein
MDFSSIVSEDKIKRAIKDGEFEGLPGMGKPLKKDDAAHVPEHLRMAYRVMKNAGMTGDEGALKKELMTIDDLIAKCADEDERDRLIERKTETRLRLDRLADKKGMFSKPASAFYKEKVYRRLSGK
>NZ_CP020352.1|WP_080131268.1|860623_862585_-|LTA-synthase-family-protein
MKRFIKERGLAFFLIAAILLWLKTYAAYVIEFNLGITNTVQQILLFVNPISSSLFFLGLGLLFKKKIQQTAIIVIHFLMSFLLYANVVYYRFFSDFITLPTIMQAKTNGGQLGDSIFSLMHPLDVLYFIDTFILIGLAVFYNRPSETTTKRPFKIVMGTSILVFLVNLAVAEIDRPELLTRSFDRNYLVKYLGTYNFTIYDAVQNIKSNSQRALASSSDVTEVENYIKANHDKPNAEYFGKAKGMNVVYISLESLQTFAIDYKIDGKEVTPFLNSLAHDNETFYFDNFFHQTGQGKTSDAEFMMDNSLYPLSQGSVFINKAQNTLQSTPAILKSQGYTSAAFHGNYKTFWNRNEMYKAIGYDKFFDATYYDMSEDKTKNYGLKDKPFFEESMPLLKSLKQPFYTKFITLSNHFPFEMDEGDTDFPAGDTGDSVVDHYFQSANYMDQAIEQFFNDLKKSGLYDNTIVVMYGDHYGISENHNKAMAKVLGKDITPYENAQLQRVPLFIHAPGVKGIKSNKYGGEVDVAPTLLHLMGVNTKDYLMSGSDLLSPEHREVIPFRNGDFVSPEYTKTNGNYYDTKTGELLNEKDIDPNIDESVKKELEMSDKIVDGDLLRFYKPKGFKAINPSDYDYTNHNPKTSESDAAQTTKSDSGQ
>NZ_CP020352.1|WP_035336955.1|859745_860498_-|type-I-methionyl-aminopeptidase
MIVTNDQELEGLKKIGRIVALAREEMKAKAAPGMSTKELDLIGKEVLEAHGAAPAPEKEYDFPGTTCISVNDEVAHGIPSESTILKEGDLINIDISAEFGGYYSDTGISFVLGKGDETLHKLCQSAESAFQKGLLQARAGKKQNQIGRAVYNEAKANGFTVIKNLTGHGIGKNLHEAPNHILNYYDPFDNALFKNGTVIALEPFVSTKAEHTIEAGDGWTFKTPDGSFVAQIEHTIVITKNEPIILTQLD
>NZ_CP020352.1|WP_080131273.1|879802_880894_-|DegT/DnrJ/EryC1/StrS-family-aminotransferase
MIRLIEPYITFEEVEAELKEIFSSGILTKGPYVRTFADHIKQYVSAKHAFLTTSATTALSMCLKSLNVQPGDEVIVSDFSFPATVNVIEDLGAKPIFADVDLETFNMLPEELESQITSRTKAVLFVDALGNPTGITNIKHICEEYGLPLVDDAACAIGSSEYGCKSGKIADLTCFSFHPRKLLTTGEGGAITTDREELKDWFSVKLNHGAAISDGQLDFMDFGYNYRLSDIQAALGIKQLQKLDDIIHRRNLQQKAYSEQLEPLGFQSQQIGPDVVHNTQSAVFKVPKNIVRDELIQYLSDCHIESTFGTYCLSGTTYYRRKYRQIKPNSIFLEQNTITLPCHDQVNVDQVVSAIQSFSQRKG
>NZ_CP020352.1|WP_026580665.1|880890_881433_-|transferase
MKQWKSEGKGEFTLSQLGGCGENVVIEDGVRIFHPENIYIGDNVYIGHDTILKGYYKHDLIIGSNSWIGQQCFIHGAGGVTIGKFAGIGPNVRIHAAYHTDPDIPDSTILFSPLTFAPIHIEENCNIGIGASILAGVTIGAHSKIGANAVVNRHIPPYSIAVGVPAKVIKNRRMKDEERS
>NZ_CP020352.1|WP_080131274.1|881450_882425_-|NAD-dependent-epimerase/dehydratase-family-protein
MSDMTELSGQHIFITGGAGFIGSSLIGKLIERNSVTVYDNFSRDSLRYKPYRDHPHLKVVQGDILDVNTLKKAIQGASHIVHAAGIAGIDTVIQNPVKTMQVNMIGSANLLEAAAGLTECKRVVCFSTSEVFGQIAFRARETSHTVLGAVGEARWTYAVSKLAEEHMAYAYFKEHGLPTVTVRPFNVYGPEQVGEGAIKTMVHRALLDEPIYIHGDGTQIRAWCYVDDMIDGILRCLTMKEAIGESFNIGNERTVITVYGLASTIIRVLGSKSQIFFREKKEADIELRIPQVNKAKEMLGFNAKVDLEEGITRTAESIRKNLDQ
>NZ_CP020352.1|WP_080131275.1|882491_883865_-|glycosyltransferase
MKDFKSDIIHCLEQKDWNKAMKRLKEWEADGSHNEPDFYFLQASLSVYLGHDHNAWLWLWRGLDLFPDNRSLNLLMGKVCLRTGREQESAAYLQKGDGAETESGPKLDLPVHEKTEPPAGQIRVLQGTMEIANQMNTLAKGLSQHGALAHTLNYYPYYLNYAADYTWSLLKERNTPAMNAKLRRLASDLLPSYDLFHFHFGTSFTLDMSDYPILKQAGKPMIMHHWGSDVRLYSTLAKTNPYAVVKTKNEALIRYHLKRISQYVQHCIVADMELYEYVKDYYEHIHMIPTMIQLDRYIPDYRSNEKPLIVHAPTSPGIKGTRHILKAVESLKEKYDFHFHLVRGVSHEQAKKIYQKADLIIDQLHIGSYGLFAVESMAMGKPVICWVSDFMKEHYPSELPLIRANPDNITEVIENVLKNRDMLPEIGQKGRKYAEVHHDMVKNSKKMLAVYQALLSG
>NZ_CP020352.1|WP_023855858.1|884018_886262_-|glycosyltransferase
MDNELEYLLDSVGLSWRETEISQLIFQHKWHDAAQKLEERLEAAPFDPGLLISAAFVYEKKQQVQRASELYDDALLFVRREHQHRLMKEKADLFKKMVRRPKIIFFVKPDLDRFLNAVVEHLSETYRTRKIAVHDLNQIDTGMQWADICWFEWCDDLLIHGSKVPSAAAKTLICRLHSYEAFTFYIKKVYWQAVDQVIFDGEHMREYVLDQVKSLKKEKTAVVPNGIKLDEYTFQQRQKGFHIAYVGYINYKKGPMLLLQAFKALIDKDPRYKLFIAGTFQDARYQLYFKQMIDELRLTDNVQFDGWQDDMNQYLEDKHYLICTSLLESQHMSAMEAMAKGIKPLIHNFYGAKTIYDESVVWNTIDEFVAMITSGDYTSLHYRSFIEEHYSYDLSMRKIEEMISGFAKQSELTVMPNPPLITVGIINYNYSNFIDECLQSVLNQLYPNIDILIVDDHSTDDSMQKISSYQKTHPHIRTICHSENTKLPDLAFKEIFQEAKGEYVLLLSADDFLPHNLVLHDFMQCFFGEEGLDYVYGDLLLVDQHGKNAGLWTYQEYGDEELVRTIFQRGGSGIVPMVGMFKASYYQQKGFTWLVDPQKPLAGDVLNCLANTKRGWKRRYLRKPVLCYRRHQNNISYQLHKRIQSLVYVIDYIVKEFDESVYFPHIKWKELEENQRQSLKYFSIGKTFWRMARRYAQNGYTRHLDQDAKKECIQPLLDKMDEYFQLSLASDNQYERYMKNIKKIQLI
>NZ_CP020352.1|WP_020450556.1|886455_887538_+|glycosyltransferase
MVTISLCMIVKNEEEVLARCLDTVKDIVDEINIVDTGSTDQTVEIAKRYTDRVFFFEWIGDFAAARNESFKHATKDYILYLDADDVLLEKDQKKLKELKETLDPSVDSVSMYYDAGTDEFGNVTLRYRRNRLVKREKNFKWKGDCHQYLEVFGNIINADISVTHKKIRHSVGRNLNIYQKKIDRGDTFTPRDYFYYGNELRENRHYEKAIESYNKNIEMKEGWIEDKVYACINKADCHRFLGDLDNELRSLFQSFEFSKTPRAETCSRIGYNFQRRREYNAAIYWYELATTLVPDSNKWSFTYPAYYTWYPHLQMCVCYYNLGDFEKSYQHNEEARKYRPDDKSVLHNKQLLEGKLGINK
>NZ_CP020352.1|WP_080131276.1|887659_888769_+|DegT/DnrJ/EryC1/StrS-family-aminotransferase
MIPLINLKRQFQTVKEDILQEFEHVLDSGQYILGPKVEELEKRIAEKLGVKEAVAVANGTDALVLTLEAFGIGKGDEVITTPFTFFATAEAVSRVGAKPVFADVDRETCNLDPEKIEENITPATKAIIPVHIFGQPADMDEIMAIAKKHGLLVIEDACQAFGASYKGRPVGSIGDAACFSFFPTKNLGTLGDGGMVTISDPDAARQLRTLRTHGTSKKYFHDKIGFNSRLDELHAAALLILLEKIDGWNEQRRKVASRYKEGLKTAEHLTLPAEKEDRTHIYHLYCIGAKDRDEFIQSLKEQNIHSGVYYPCCLHLQTVYSSLQYKKGDFPIAESLSETLFAIPMDPFLAAEEQEQIISALLKKGGGGK
>NZ_CP020352.1|WP_080131277.1|888765_889830_+|Gfo/Idh/MocA-family-oxidoreductase
MTVHFGLIGCGYMSRKHLQALAGCGDAKLSAVSDLQVERMKEAEQYYASFAGDESRMTRYQQYQALLSDSKIEAVIIAAVSGLHAEMAKAALQAGKHVIVEKPITLSLQDADELIELAEQNGLKLMVCHQMRHRPIMKKLKETIERGKLGRIYLGTVSLRLNRSPAYYEAATWRGSWEKDGGMLVNQGIHLIDLLQWFLGDVKGVYGDILGGSGAKETEDVAVGVLTFASQAKGVVEANIVTQPNNIGSSLSIFGEHGTISLEGPALNRISRWYIHGEKDAAEEAAQLIDLGGEQTAMYQNFISAVKRQDTLLVDGYEGKKAIETIFGIYQSVLQKQPVQLPLSSFQTSDMKNK
>NZ_CP020352.1|WP_080131278.1|889915_891211_+|nucleotide-sugar-dehydrogenase
MSKQEMHNETIAVIGLGFVGLPLAMLLISKGIRVIGVDLDKKKVEALRKGNSYISDINDQAVQDGIGTGFFRPVSDYGLIGEADAVIICVPTPLNDDAGPDLTYVKRAAQSIGGHLKKGQLVVLESSTYPGTTRDIVKPILEESGLVCGKDFYLAYSPERVDPGNQFPIEVVPKVVGGATEACLEKVHQLYSGIYEEVVPVTSPETAELTKLLENTYRFINISFINEFAMICDKLGVNVWEVIEAASTKPYGFAAFYPGPGIGGHCIPVDPLYLQFVMKQSGLSSDFIELSEATNDRIVRYIAERSLQLAAKDKSTDARILIYGVTYKRNIADVRESKSLDIIEKLIGMGADVSYHDPHVPELDLSGKKMASMPIDSEMLKAYDCVVVLTDHDALPKELLLNHATVIFDTRGVLREAAGKAKVIQLGNGCS
>NZ_CP020352.1|WP_080131279.1|891291_892203_+|alpha/beta-hydrolase
MWIGILLMVFGIGISIFAVSLIVSYLFVMPNRSTYEETFFSGLKHGEIIDDVFESHDKEELFLTAGDGCRLHALLFPVQKSRKVVIVSHGIKWSLFGGYKYVELFQSIGYNVLLCDSRCHGLSGGSHVSYGFYEKDDLARWADELENRFGKDLWIGVLGESLGAAAAIELMKQDRRIKFCIADSCFSDLTELCRFQLKAAVKLSVPFLIPLASGLIKRRHGWSLADISPVSNLEQSDTPLFIIHGTNDQLVPPEMAKSLYDRKKGIKKLYLIEGAGHVGGFRHDPEEYLSRIKDFLLKIEPAN

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Crispr_ID: NZ_CP020352_3

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

NZ_CP020352_3

2245097-2245195

Orphan

Consensus_repeat	Method
TCAGGCAGCGATTCTTTTAGCGTT	CRISPRCasFinder

1 spacers

csa3,WYL

The CRISPR arrays of NZ_CP020352_3

>merge|NZ_CP020352|3|2245097-2245195|CRISPRCasFinder
TCAGGCAGCGATTCTTTTAGCGTTTCTACAACTGACCGCTCGACACTTTTCGTAGAGCCGGCGGCCGTCCCACTATCAGACAGCGATTCTTTTAGCGTT

>NZ_CP020352|3|2|2245097-2245195|CRISPRCasFinder
TCAGGCAGCGATTCTTTTAGCGTT	TCTACAACTGACCGCTCGACACTTTTCGTAGAGCCGGCGGCCGTCCCACTA
TCAGACAGCGATTCTTTTAGCGTT

Protein	Signature genes	Signature genes Name	Protein_function
NZ_CP020352.1\|WP_025811320.1\|2243991_2244639_-\|peptidase	unknown	unknown	gnl\|CDD\|370127
NZ_CP020352.1\|WP_020451772.1\|2255158_2255680_-\|hypothetical-protein	unknown	unknown	unknown
NZ_CP020352.1\|WP_003182557.1\|2238967_2239300_-\|Zn(II)-responsive-metalloregulatory-transcriptional-repressor-CzrA	csa3	COG0640_csa3_CAS-I-A	gnl\|CDD\|238042
NZ_CP020352.1\|WP_020451755.1\|2236964_2237249_-\|hypothetical-protein	unknown	unknown	gnl\|CDD\|378913
NZ_CP020352.1\|WP_079289250.1\|2242134_2242833_+\|lysozyme-family-protein	unknown	unknown	gnl\|CDD\|381612
NZ_CP020352.1\|WP_033155124.1\|2238417_2238927_+\|hypothetical-protein	unknown	unknown	unknown
NZ_CP020352.1\|WP_025811322.1\|2240350_2240755_-\|hypothetical-protein	unknown	unknown	gnl\|CDD\|340167
NZ_CP020352.1\|WP_023855697.1\|2249400_2250366_+\|LD-carboxypeptidase	unknown	unknown	gnl\|CDD\|132883
NZ_CP020352.1\|WP_023855705.1\|2239385_2240210_-\|hypothetical-protein	unknown	unknown	gnl\|CDD\|293813
NZ_CP020352.1\|WP_020451756.1\|2237302_2238238_-\|YafY-family-transcriptional-regulator	WYL	COG2378_WYL_CAS-I:CAS-III	gnl\|CDD\|225253
NZ_CP020352.1\|WP_020451769.1\|2250397_2251987_-\|solute:sodium-symporter-family-transporter	unknown	unknown	gnl\|CDD\|236699
NZ_CP020352.1\|WP_011201670.1\|2256084_2256312_+\|putative-motility-protein	unknown	unknown	gnl\|CDD\|379452
NZ_CP020352.1\|WP_023855698.1\|2248846_2249356_+\|GNAT-family-N-acetyltransferase	unknown	unknown	gnl\|CDD\|366181
NZ_CP020352.1\|WP_023855703.1\|2240754_2242092_+\|APC-family-permease	unknown	unknown	gnl\|CDD\|223605
NZ_CP020352.1\|WP_023855696.1\|2252147_2252957_-\|LysM-peptidoglycan-binding-domain-containing-protein	unknown	unknown	gnl\|CDD\|226112
NZ_CP020352.1\|WP_080131569.1\|2253193_2254975_-\|DNA-helicase-RecQ	unknown	unknown	gnl\|CDD\|223588
NZ_CP020352.1\|WP_020451766.1\|2247636_2248617_-\|hypothetical-protein	unknown	unknown	unknown
NZ_CP020352.1\|WP_020451764.1\|2245563_2246781_-\|glycosyltransferase-family-4-protein	unknown	unknown	gnl\|CDD\|340831
NZ_CP020352.1\|WP_020451761.1\|2242966_2243953_-\|NAD-dependent-epimerase/dehydratase-family-protein	unknown	unknown	gnl\|CDD\|367154
NZ_CP020352.1\|WP_020451765.1\|2246950_2247484_-\|DUF420-domain-containing-protein	unknown	unknown	gnl\|CDD\|225204

Protein	Function_ID	Function_description	E-value
NZ_CP020352.1\|WP_025811320.1\|2243991_2244639_-\|peptidase	gnl\|CDD\|370127	pfam08795, DUF1796, Putative papain-like cysteine peptidase (DUF1796).	1.70321e-34
NZ_CP020352.1\|WP_003182557.1\|2238967_2239300_-\|Zn(II)-responsive-metalloregulatory-transcriptional-repressor-CzrA	gnl\|CDD\|238042	cd00090, HTH_ARSR, Arsenical Resistance Operon Repressor and similar prokaryotic, metal regulated homodimeric repressors. ARSR subfamily of helix-turn-helix bacterial transcription regulatory proteins (winged helix topology). Includes several proteins that appear to dissociate from DNA in the presence of metal ions.	3.85328e-19
NZ_CP020352.1\|WP_020451755.1\|2236964_2237249_-\|hypothetical-protein	gnl\|CDD\|378913	pfam12674, Zn_ribbon_2, Putative zinc ribbon domain. This domain appears to be a zinc binding DNA-binding domain.	9.76662e-33
NZ_CP020352.1\|WP_079289250.1\|2242134_2242833_+\|lysozyme-family-protein	gnl\|CDD\|381612	cd16891, CwlT-like, CwlT-like N-terminal lysozyme domain and similar domains. CwlT is a bifunctional cell wall hydrolase containing an N-terminal lysozyme domain and a C-terminal NlpC/P60 endopeptidase domain (gamma-d-D-glutamyl-L-diamino acid endopeptidase), and has been implicated in the spread of transposons. Proteins similar to this family include the soluble and insoluble membrane-bound LTs in bacteria, the LTs in bacteriophage lambda, as well as the eukaryotic "goose-type" lysozymes (goose egg-white lysozyme; GEWL).	5.7057e-77
NZ_CP020352.1\|WP_025811322.1\|2240350_2240755_-\|hypothetical-protein	gnl\|CDD\|340167	pfam17452, YnfE, Uncharacterized protein YnfE. This is a family of unknown function found in Bacillus.	1.17769e-33
NZ_CP020352.1\|WP_023855697.1\|2249400_2250366_+\|LD-carboxypeptidase	gnl\|CDD\|132883	cd07062, Peptidase_S66_mccF_like, Microcin C7 self-immunity protein determines resistance to exogenous microcin C7. Microcin C7 self-immunity protein (mccF): MccF, a homolog of the LD-carboxypeptidase family, mediates resistance against exogenously added microcin C7 (MccC7), a ribosomally-encoded peptide antibiotic that contains a phosphoramidate linkage to adenosine monophosphate at its C-terminus. The plasmid-encoded mccF gene is transcribed in the opposite direction to the other five genes (mccA-E) and is required for the full expression of immunity but not for production. The catalytic triad residues (Ser, His, Glu) of LD-carboxypeptidase are also conserved in MccF, strongly suggesting that MccF shares the hydrolytic activity with LD-carboxypeptidases. Substrates of MccF have not been deduced, but could likely be microcin C7 precursors. The possible role of MccF is to defend producer cells against exogenous microcin from re-entering after having been exported. It is suggested that MccF is involved in microcin degradation or sequestration in the periplasm.	3.97567e-106
NZ_CP020352.1\|WP_023855705.1\|2239385_2240210_-\|hypothetical-protein	gnl\|CDD\|293813	cd07727, YmaE-like_MBL-fold, uncharacterized subgroup which includes Bacillus subtilis YmaE and related proteins; MBL-fold metallo hydrolase domain. Includes the uncharacterized Bacillus subtilis YmaE and Nostoc all1228 proteins.Members of this subgroup belong to the MBL-fold metallo-hydrolase superfamily which is comprised mainly of hydrolytic enzymes which carry out a variety of biological functions. The class B metal beta-lactamases (MBLs) from which this fold was named are only a small fraction of the activities which are included in this superfamily. Activities carried out by superfamily members include class B beta-lactamases, hydroxyacylglutathione hydrolases, AHL (acyl homoserine lactone) lactonases, persulfide dioxygenases, flavodiiron proteins, cleavage and polyadenylation specificity factors such as the Int9 and Int11 subunits of Integrator, Sdsa1-like and AtsA-like arylsulfatases, 5'-exonucleases human SNM1A and yeast Pso2p, ribonuclease J and ribonuclease Z, cyclic nucleotide phosphodiesterases, insecticide hydrolases, and proteins required for natural transformation competence. Classical members of the superfamily are di-, or less commonly mono-, zinc-ion-dependent hydrolases, however the diversity of biological roles is reflected in variations in the active site metallo-chemistry. .	2.65923e-68
NZ_CP020352.1\|WP_020451756.1\|2237302_2238238_-\|YafY-family-transcriptional-regulator	gnl\|CDD\|225253	COG2378, COG2378, Predicted transcriptional regulator [Transcription].	1.66695e-73
NZ_CP020352.1\|WP_020451769.1\|2250397_2251987_-\|solute:sodium-symporter-family-transporter	gnl\|CDD\|236699	PRK10484, PRK10484, putative transporter; Provisional.	0
NZ_CP020352.1\|WP_011201670.1\|2256084_2256312_+\|putative-motility-protein	gnl\|CDD\|379452	pfam14070, YjfB_motility, Putative motility protein. This family of proteins is regulated in B. subtilis by SigD, and is likely to be involved in motility or flagellin production, Proteins in this family are approximately 60 amino acids in length, and contain two highly conserved asparagine residues.	4.76798e-09
NZ_CP020352.1\|WP_023855698.1\|2248846_2249356_+\|GNAT-family-N-acetyltransferase	gnl\|CDD\|366181	pfam00583, Acetyltransf_1, Acetyltransferase (GNAT) family. This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.	0.00346203
NZ_CP020352.1\|WP_023855703.1\|2240754_2242092_+\|APC-family-permease	gnl\|CDD\|223605	COG0531, PotE, Amino acid transporters [Amino acid transport and metabolism].	3.89024e-37
NZ_CP020352.1\|WP_023855696.1\|2252147_2252957_-\|LysM-peptidoglycan-binding-domain-containing-protein	gnl\|CDD\|226112	COG3584, COG3584, Uncharacterized protein conserved in bacteria [Function unknown].	1.10066e-46
NZ_CP020352.1\|WP_080131569.1\|2253193_2254975_-\|DNA-helicase-RecQ	gnl\|CDD\|223588	COG0514, RecQ, Superfamily II DNA helicase [DNA replication, recombination, and repair].	0
NZ_CP020352.1\|WP_020451764.1\|2245563_2246781_-\|glycosyltransferase-family-4-protein	gnl\|CDD\|340831	cd03801, GT4_PimA-like, phosphatidyl-myo-inositol mannosyltransferase. This family is most closely related to the GT4 family of glycosyltransferases and named after PimA in Propionibacterium freudenreichii, which is involved in the biosynthesis of phosphatidyl-myo-inositol mannosides (PIM) which are early precursors in the biosynthesis of lipomannans (LM) and lipoarabinomannans (LAM), and catalyzes the addition of a mannosyl residue from GDP-D-mannose (GDP-Man) to the position 2 of the carrier lipid phosphatidyl-myo-inositol (PI) to generate a phosphatidyl-myo-inositol bearing an alpha-1,2-linked mannose residue (PIM1). Glycosyltransferases catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. The acceptor molecule can be a lipid, a protein, a heterocyclic compound, or another carbohydrate residue. This group of glycosyltransferases is most closely related to the previously defined glycosyltransferase family 1 (GT1). The members of this family may transfer UDP, ADP, GDP, or CMP linked sugars. The diverse enzymatic activities among members of this family reflect a wide range of biological functions. The protein structure available for this family has the GTB topology, one of the two protein topologies observed for nucleotide-sugar-dependent glycosyltransferases. GTB proteins have distinct N- and C- terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. The members of this family are found mainly in certain bacteria and archaea.	5.41588e-63
NZ_CP020352.1\|WP_020451761.1\|2242966_2243953_-\|NAD-dependent-epimerase/dehydratase-family-protein	gnl\|CDD\|367154	pfam02719, Polysacc_synt_2, Polysaccharide biosynthesis protein. This is a family of diverse bacterial polysaccharide biosynthesis proteins including the CapD protein, WalL protein, mannosyl-transferase, and several putative epimerases (e.g. WbiI).	9.8559e-117
NZ_CP020352.1\|WP_020451765.1\|2246950_2247484_-\|DUF420-domain-containing-protein	gnl\|CDD\|225204	COG2322, COG2322, Predicted membrane protein [Function unknown].	3.45867e-63

>NZ_CP020352.1|WP_025811320.1|2243991_2244639_-|peptidase
MNLKEIKGQYNAVFSLGHLCLTAIQLKNNDLRPFSGPLDWVTIHSLTDLNLLLRNRFTGFMEPKNLRVIGYNTGKKKEEGSTIYVSDDFYNIGSVHDFDANQNTLDHLATYPEVIEKYNRRIDRFIEKMSTSKRILFVRTEGEGSFKEAQELELVLSDLVQHDFSVLLVKHSKVNGIVEKNWPLEKTCTVELPDKEIWESNNDLWASLFKGITLV
>NZ_CP020352.1|WP_020451761.1|2242966_2243953_-|NAD-dependent-epimerase/dehydratase-family-protein
MFENKTIFITGGTGSWGQELILQLLSLNPKKLIVFSRNEERQVQMRRQIEDPRLTFFIGDIRDRKALNTGLKGVDYVFHLAALKHVPVCEEQPLEALKTNVTGTENVIEAAIENGVKKVINVSTDKAADPANFYGMTKAIGEKLIVYANHLYSDTDFICVRGGNVLGSSGSVLQLFKDQIRKKNQVSITDKRMTRFFLTKEKTIRLLLKAAESGQGGEIFIMNSPACRILDMAEVLIEAYGNEKTSIAETGARPGEKLHELLISPHERQHAVSFTNDLAVVLPAIKMPELHKKYADCQPLETDHLSSKDFLISKKEVKEMLKEGGFLE
>NZ_CP020352.1|WP_079289250.1|2242134_2242833_+|lysozyme-family-protein
MRLYTVLHQGATEVKPLKKKKSGCLAGAGCLSFFVFVPLIIFFAISGLEQLKWLPLDTESNILERLKDYKPLVEEELDEQDLSQYTALLLGMMYQESKGRGGDPMQSSESLGMKRNEIDDPKASIKQGVRHFTAMYKKGQKKGVDIETIIQSYNMGSGYIDYVSKHGGKHSEKLAKAFSEEQVKKNPDLYNCGGDTNNFRYPYCYGDYSYTEKVMERTKKVEEHIEGLTLEH
>NZ_CP020352.1|WP_023855703.1|2240754_2242092_+|APC-family-permease
MKNETALKRSLTLGPLVVIGLAYMDPLVVFDSYGVVAQLTKGHVPAAYIFTVAALLLTALSYGSMVKAYPKAGSVYTYAYESIHPHAGFLAGWTIMLDYLFLPMVNFAIGSAYLTAAFPEVPHGFWVVLLALSITVVNILGIRLTANITGLFVAFQVIVALTFAGFMIAGLADGLGTGELVSADPFFRSDIEPSLIVSGATILCFSFLGFDAISTMSEETVNPKKNIPLAIFLTVAIGGTLFTAISYLMQQIYPSYDSFQHADSASLEIALYTGGAFLKSFFLAGTMVAVVSSSLSSHASASRLLYAMGRDSSLPKRFFGYVHPTLKTPVFNIMFIGLISLTAMIGELEVIYSFISFGALIGFIFVHLSVIGHYYIRKQERGFLKTLRYLVLPFLGASFCGWLLLSISKNALMIGAGWLIAGFIYFLFRIRTLPNVTFGFDRDPR
>NZ_CP020352.1|WP_025811322.1|2240350_2240755_-|hypothetical-protein
MTSKSPYVKNTEIKTYFICFFALEQLYKTSFFRIKIATVANPLKKGKEYEGMDILRGYVEAVKRNMETMNASDYDGKEEDLRRQQEELERYEQRLKEKSASADRFDQVVDAAVDCAAGDISFSQLEHMYQQEGS
>NZ_CP020352.1|WP_023855705.1|2239385_2240210_-|hypothetical-protein
MTNYICTTCGVQYDAAPKEPAECIICNEERQYVNHSGQAWTTLEEMKQSGRFQNEIALEEERLYSLTTTPEFGIGQSAYLIQEPGFNLLWDCITYLDQRTIENIERMGGVQAIALSHPHYYAAQTEWAEMFDAPIYIHEDDKQWVQRPSSRIVFWSGDSLKLSSGLTLHRLGGHFKGGAVCHWANGNGGKGILLSGDIIQVVQDRRWVSFMYSYPNLIPLPAGKVEQIADQLKNVPFNRLYGAFRRSVKNHAGERVQLSAARYAEALLGTLFST
>NZ_CP020352.1|WP_003182557.1|2238967_2239300_-|Zn(II)-responsive-metalloregulatory-transcriptional-repressor-CzrA
MSEHNQETTEKQVRAELDEETLFLVSQTFKALSDPTRIKILHLLSQGEHSVNEIAEKLSLLQSTVSHQLRFLKNLRLVKSRRAGTSIFYSPEDQHVLDVLQQMIDHTQHD
>NZ_CP020352.1|WP_033155124.1|2238417_2238927_+|hypothetical-protein
MYYFLQFFAIVSFVYIKFKRSVGYQPLKPARMLFRIILFSGIFVFLLTMSALHPLSYFYDLIGIALGLILTAYALKHVSIENRDGILYFRTHLWIELIVLLLFLYRFLYRIAEIGQLQTAVSNGGSAAYGALFAQDPATMIGFFVLAVYYVGFSFFVLKKGRNEEKRSA
>NZ_CP020352.1|WP_020451756.1|2237302_2238238_-|YafY-family-transcriptional-regulator
MKIDRLLAIVILLINKRQVQAKELAGMFEVSVRTIYRDIEAINQAGIPIVTLQGAGGGISIMERFRLENKLLSADELAAITTALESVSSTYEVLHHGSALEKIRFLVSEEEMPEFRKKTEKWFVDISSWGESPVIKEKIRLLDEAADSTRAVSFTYVNREGQTGTRKAEPHTLVLKGRHWYLYAFCLKRQAFRFFKLLRMKDVQMLEEKFERKEVDLSSLPWETSWYKPDNMTELTLLIKPSGESLAREWFGSEALTYENGQCVATVSYPEDQWLYGFLLQFGTHIEVLTPPRIRQKIKNLANQIVHLYET
>NZ_CP020352.1|WP_020451755.1|2236964_2237249_-|hypothetical-protein
MEHHICQSCGMPMTSELFGTEKDGGKNRDYCMYCYENGAYTHPDATLKEMIDICVPYIKEEGKTEAEARSLMERTLPHLKRWASDTQTKGGQRR
>NZ_CP020352.1|WP_020451764.1|2245563_2246781_-|glycosyltransferase-family-4-protein
MKILLATYWSIPHVGGVWTYMVQLKEKLESLGHEVDLLGYGEENSIIHIVNENRSIAKDQLLPLVKAMMSEEDFPEIYANQLVSYTELQRNAYELGAAYLGLDKYDIIHAQDVISSACIKRIMPKDKVLVTSIHGSVTYEIRDQLNNIHRSPTSYIAKEYFDHLEHTGAMSAETVIVANNWLKDILTEEFAVPDGKIHVSQYGYDIDGFLARMNTKTADTPETDKKVILFTSRMIGNKGIHYLIEALSQLKDTRNDWVCWMAGEGEKLAGLKIQSEQLGLVDDVVFLKNREDVPYLLSLADIYVLPSLLDNQPLSLIEAQIAGVPAVVSDAGGLPEMVEHEETGLIVPKGDAAALSSSLERLLKDDDFRNKLGINAKEFAKKHWDMDQAVTRILDIYQNTNGKEE
>NZ_CP020352.1|WP_020451765.1|2246950_2247484_-|DUF420-domain-containing-protein
MTHTQKNKNYTGIVVALTIIINGVIALLFFMPKSDKFAHLDLTFLPLLNAVCNSFTFIFLVAALIMIKQKNIKAHKRFIFAAFTTTFIFLVSYLTYHSLMPDTHFGGEGIIRPIYYFILITHIVLSALIVPLALFTLFRGLSMQVERHRKIARWTMPLWLYVSLTGVLVYMMISPYY
>NZ_CP020352.1|WP_020451766.1|2247636_2248617_-|hypothetical-protein
MKKRIFSLLMLMSAFVLVLAACQQADPKGSYATAYKKLISAKSYEFSSDIGLKVDASNLGAEDKQVADMLNDATLTISGKTNAETKQSEAVIKAKLKVQNMSLDFDIPVYMDEKKQVGYIKIDSVLDSFGFFLGTAGMNFDSVKGKYLEFPLEDDKTSSQDTDELQKQLMESIKEAADNLPADKFEKADLTDKEKEQGAAQKVAFSFSDKEIKAAVVKFIEIAGKASGEAVPKSELNKIKDGLKDVTFKKFDVTSTIDDKENLLTENADISLSVDSKKAPQSLGITASTTYKNIDGDVKFEHKPNKEDIVTEEEFEKLMAKDAMNF
>NZ_CP020352.1|WP_023855698.1|2248846_2249356_+|GNAT-family-N-acetyltransferase
MITDEELQKDSSGLQKELNELQFQLFRMRENMKDISKDSRVLGIDQSKDDEWMIVHSIDDGRTCKIMLSDCQSPYRGRCDFSLVASYKEEKCAIHIGDIKGPAGYGYGSICMKYLKEKAREHNIPVITGDIAERDWDHVDRLIHFYEKHHFEVTIDPGLKSGEIQWQDL
>NZ_CP020352.1|WP_023855697.1|2249400_2250366_+|LD-carboxypeptidase
MFPEKLQPGDEIRVIAPSRSLSLLNTDKIEKARTVLNNLGFRISFSAHANEIDECSSSSIQSRVSDLHDSFSDQRVKGILTVLGGYNSNQLLSHLNYELIKANPKVFCGFSDITALSAAIYAKTGLVTYSGPHFSSFSMGYGLNYMTDYFLKCCCASGVYDVEPSSMWCEEIETEECPKQNSGPVVINEGSADGKIIGGNLCTLNLLQGTEYFPDIKNAVLFLEDDWESPAAFFDRDLQSLLHLPDFSGVKAVLIGRFQTASAVTLERLKQIVQTKKELSQIPVIANIDFGHTTPFFTFPIGGRCRIDAQKEHVSLCITKH
>NZ_CP020352.1|WP_020451769.1|2250397_2251987_-|solute:sodium-symporter-family-transporter
MLWIIMTFLLINAGIFIFSYLRTKDINLKTSDGYFLGGRSLTALYIGSSLLLTNLSTEQMVGLNGQSYSGTMSAMAWEVTAPLALIMLAFVFLPRYLSSGVTTIPDFLEKRYDQRTRQIVSILLLLGYATSFLPTVLYSGALVLDQVFNISSALGISTFATVFFISLVIGAASCCYIFFGGLKAAASADAIYGAGLILGGLFIFVLGIMAIGDGSLLNGYTELTSVHKEKLNAITADNAAEVPWPTIFTGLLVNNLFYWAVNQSIVQRALGAKNLAEGQKGALLAGVFKTIGVFYLVLPGVIAYHLYGGHIENADTVYPKLIVDLLPTALIGVFAAILFGAILSSFNGSLNSTITLFTLDLYKPIFKPEAKEEELVKTGRIFVVILAAVSIIIAPFILFAPSGLYNYLQEMFGFFNVPVLAAVVVGFFTKKVPALAVKWAIFAHIVLYTISKIYFGHIHFLYILAVLFPFSVLLMLFIGKLKPRPSEFKLEKNAKVDLRPWKHAKLASILVICLMACIYLLFSPWGLAK
>NZ_CP020352.1|WP_023855696.1|2252147_2252957_-|LysM-peptidoglycan-binding-domain-containing-protein
MKKTFMSFVAVAALSSTAFGASASAKEVTVQKGDTLWGISQKQGVNLQDLKEWNQLSSDLIIPGQKLNVSEKQTEEKKQYTIKKGDTLWKIAQQFGVSVNDLKNWNNIKSDIIYPNTSITVDGQATVQAAAAQPAETKPAVQKEAKVEKAAPAPKQEKETASRSNESQSSQSAAKELTVTATAYTANDGGMTGVTATGFDLNANKNAKIIAVDPNVIPLGSKVYVEGYGEATAADTGGAIKGNKIDVFVPSKSAAKNWGVKTVKVKVLK
>NZ_CP020352.1|WP_080131569.1|2253193_2254975_-|DNA-helicase-RecQ
MLEKAEALLQHYYGYPSLRPGQKKAIQSVVSEKRDTACIMPTGGGKSICYQIPALLMEGTTIVISPLISLMKDQVDALNQLGIQAAYVNSSLGHGEIAERLSVLKEGGYKLFYVTPERLTSPDFIRVLSRIHIPLTAIDEAHCISQWGHDFRPSYRHIESFLNSLGTRPVVLALTATATPEVHEDICTQLGIEKESTVFTGFSRDNLTFKILKGENKDRFIESYVEKNRSEAGIIYTATRKEAERISSRLSQKGIAAGCYHGGMNDEERDRQQDLFLNDEISVMTATSAFGMGIDKSNIRYVIHAQIPKNMESYYQEAGRAGRDGLDSECILLFSPQDIRLQRFLIEQSTEDEDIRAQDVKKLRQMVDFCHTEGCLERFILSYFGEVSTEDCGRCSSCLDTRSITDVTKEAQMVLSCIIRMGERFGKTMVAQVLAGSKNKKVAENGFQQLSTYGILKNRSIADISDFIEFLIADEYIQMSDGTYPVLFVANKGRNVLLGREAVMRKEKMQTSQIVKDDELFERLRALRRKLAQEQGVPPFVIFSDETLREMSGKVPLTDEELLSVKGVGEQKRVKYGAVFLQELQAYKTEKEA
>NZ_CP020352.1|WP_020451772.1|2255158_2255680_-|hypothetical-protein
MDWLEFVSATVQSLAWPATLMMCVLLLKEPLSERMKELIKLKYKDFELEFDKRLQKLVSVREKRLSGLTSVRSAPAGENMIESAWSDVHNVLRETASTFDQNAIRLEGDELIQMLHDRGMIQDDAASVLSELSALRRDASDRASITGALAYKTLCLEEAKRLQLLAEQQTAIK
>NZ_CP020352.1|WP_011201670.1|2256084_2256312_+|putative-motility-protein
MSKVLTSLTDTHVKEVVMLDIAVLSMAMSQAALYQNVQMSLTKTALDTAEQSAQQLVDMVQAPHPVSGHHIDLKG

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Crispr_ID: NZ_CP020352_4

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

NZ_CP020352_4

2391771-2391986

Unclear

Consensus_repeat	Method
AAGCCTTGCAAGAAGGATTACGATTATTGCAAAAAGGATTATTACTACCATGAG	CRISPRCasFinder

1 spacers

DinG,cas3

The CRISPR arrays of NZ_CP020352_4

>merge|NZ_CP020352|4|2391771-2391986|CRISPRCasFinder
GATCCTTACAAGAAAGATCACGATTATTGCAAAAAGGACTACTACTACCATGAGCCTTGCAAAAAAGACTTCTATTACCATGAACCTTGCAAGAAGGATTACGATTATTGCAAAAAGGATTATTACTACCATGAGGATTACAAAAAAGACTACGATTACCACAAGCCTTGCAAGAAGGATTACGATTATTGCAAAAAGGATTATTACTACCATGAG

>NZ_CP020352|4|3|2391771-2391986|CRISPRCasFinder
GATCCTTACAAGAAAGATCACGATTATTGCAAAAAGGACTACTACTACCATGAG	CCTTGCAAAAAAGACTTCTATTACCATGAACCTTGCAAGAAGGATTACGATTATTGCAAAAAGGATTATTACTACCATGAGGATTACAAAAAAGACTACGATTACCAC
AAGCCTTGCAAGAAGGATTACGATTATTGCAAAAAGGATTATTACTACCATGAG

Protein	Signature genes	Signature genes Name	Protein_function
NZ_CP020352.1\|WP_003182873.1\|2393998_2394295_-\|cell-division-regulator-GpsB	unknown	unknown	gnl\|CDD\|237616
NZ_CP020352.1\|WP_020451911.1\|2395043_2395313_-\|spore-coat-protein-CotD	unknown	unknown	gnl\|CDD\|371387
NZ_CP020352.1\|WP_025811239.1\|2396873_2399153_-\|DEAD/DEAH-box-helicase	cas3	mkCas0139_cas3_CAS-I	gnl\|CDD\|224126
NZ_CP020352.1\|WP_080131430.1\|2386816_2388322_-\|carboxypeptidase-M32	unknown	unknown	gnl\|CDD\|341054
NZ_CP020352.1\|WP_035338546.1\|2382286_2382790_-\|hypothetical-protein	unknown	unknown	gnl\|CDD\|224669
NZ_CP020352.1\|WP_026579404.1\|2390836_2391199_-\|hypothetical-protein	unknown	unknown	unknown
NZ_CP020352.1\|WP_048350159.1\|2382797_2383895_-\|chalcone-synthase	unknown	unknown	gnl\|CDD\|225958
NZ_CP020352.1\|WP_020451912.1\|2395554_2396868_-\|nucleic-acid-binding-enzyme-YprB	unknown	unknown	gnl\|CDD\|225895
NZ_CP020352.1\|WP_048350162.1\|2385874_2386459_-\|xanthine-phosphoribosyltransferase	unknown	unknown	gnl\|CDD\|181705
NZ_CP020352.1\|WP_025811237.1\|2399938_2400358_+\|hypothetical-protein	unknown	unknown	gnl\|CDD\|223149
NZ_CP020352.1\|WP_050820871.1\|2388434_2390354_-\|ATP-dependent-DNA-helicase	DinG	COG1199_DinG_CAS-IV-A	gnl\|CDD\|224120
NZ_CP020352.1\|WP_025811247.1\|2383936_2384488_-\|GNAT-family-N-acetyltransferase	unknown	unknown	gnl\|CDD\|224584
NZ_CP020352.1\|WP_025811238.1\|2399256_2399763_-\|PTS-glucose-transporter-subunit-IIA	unknown	unknown	gnl\|CDD\|376324
NZ_CP020352.1\|WP_025811241.1\|2394375_2394927_-\|DUF1273-domain-containing-protein	unknown	unknown	gnl\|CDD\|237461
NZ_CP020352.1\|WP_025811236.1\|2400376_2400820_-\|hypothetical-protein	unknown	unknown	gnl\|CDD\|372950
NZ_CP020352.1\|WP_003182857.1\|2382127_2382223_-\|Fur-regulated-basic-protein-FbpC	unknown	unknown	gnl\|CDD\|380216
NZ_CP020352.1\|WP_050820870.1\|2392312_2393464_-\|class-I-SAM-dependent-RNA-methyltransferase	unknown	unknown	gnl\|CDD\|223194
NZ_CP020352.1\|WP_011198064.1\|2400998_2401199_+\|hypothetical-protein	unknown	unknown	gnl\|CDD\|290882
NZ_CP020352.1\|WP_035338547.1\|2378419_2381983_-\|dynamin	unknown	unknown	gnl\|CDD\|206739
NZ_CP020352.1\|WP_048350161.1\|2384561_2385878_-\|purine-permease	unknown	unknown	gnl\|CDD\|274468

Protein	Function_ID	Function_description	E-value
NZ_CP020352.1\|WP_003182873.1\|2393998_2394295_-\|cell-division-regulator-GpsB	gnl\|CDD\|237616	PRK14127, PRK14127, cell division regulator GpsB.	2.30627e-48
NZ_CP020352.1\|WP_020451911.1\|2395043_2395313_-\|spore-coat-protein-CotD	gnl\|CDD\|371387	pfam11122, Spore-coat_CotD, Inner spore coat protein D. This family is conserved in the Enterobacteriaceae family. CotD is an inner spore coat protein that is expressed in the middle phase of mother cell gene expression. Along with CotD, CotH, CotS and CotT it is assumed to assemble into the loose skeleton of the matrix, between the shells of SpoIVA and CotE. Coat proteins do not share much sequence similarity between species, but this does not imply they do not share secondary, tertiary, or quaternary features.	6.15083e-12
NZ_CP020352.1\|WP_025811239.1\|2396873_2399153_-\|DEAD/DEAH-box-helicase	gnl\|CDD\|224126	COG1205, COG1205, Distinct helicase family with a unique C-terminal domain including a metal-binding cysteine cluster [General function prediction only].	0
NZ_CP020352.1\|WP_080131430.1\|2386816_2388322_-\|carboxypeptidase-M32	gnl\|CDD\|341054	cd06460, M32_Taq, Peptidase family M32, which includes thermostable carboxypeptidases TaqCP, PfuCP and FisCP. Peptidase family M32 is a subclass of metallocarboxypeptidases, distributed mainly in bacteria and archaea, whose members contain a HEXXH motif that participates in coordinating a divalent cation such as Zn2+ or Co2+. It includes the thermostable carboxypeptidases (E.C. 3.4.17.19) from Thermus aquaticus (TaqCP) and Pyrococcus furiosus (PfuCP), which have broad specificities toward a wide range of C-terminal substrates that include basic, aromatic, neutral and polar amino acids. These enzymes have a similar fold to the M3 peptidases such as neurolysin and the M2 angiotensin converting enzyme (ACE). The keratin-degrading extremophilic eubacterium Fervidobacterium islandicum M32 carboxypeptidase (FisCP) plays an important role in cellular metabolism, and significantly enhances the degradation of native chicken feathers. It has been shown to mainly cleave the C-termini of peptides with a basic amino acid sequence. Novel M32 peptidases from some eukaryotes: protozoa Trypanosoma cruzi, a causative agent of Chagas' disease, and Leishmania major, a parasite that causes leishmaniasis, have been identified, thus making these enzymes an attractive potential target for drug development against these organisms.	0
NZ_CP020352.1\|WP_035338546.1\|2382286_2382790_-\|hypothetical-protein	gnl\|CDD\|224669	COG1755, COG1755, Uncharacterized protein conserved in bacteria [Function unknown].	2.44809e-49
NZ_CP020352.1\|WP_048350159.1\|2382797_2383895_-\|chalcone-synthase	gnl\|CDD\|225958	COG3424, BcsA, Predicted naringenin-chalcone synthase [Secondary metabolites biosynthesis, transport, and catabolism].	8.79143e-154
NZ_CP020352.1\|WP_020451912.1\|2395554_2396868_-\|nucleic-acid-binding-enzyme-YprB	gnl\|CDD\|225895	COG3359, COG3359, Predicted exonuclease [DNA replication, recombination, and repair].	2.3008e-97
NZ_CP020352.1\|WP_048350162.1\|2385874_2386459_-\|xanthine-phosphoribosyltransferase	gnl\|CDD\|181705	PRK09219, PRK09219, xanthine phosphoribosyltransferase; Validated.	3.15758e-121
NZ_CP020352.1\|WP_025811237.1\|2399938_2400358_+\|hypothetical-protein	gnl\|CDD\|223149	COG0071, IbpA, Molecular chaperone (small heat shock protein) [Posttranslational modification, protein turnover, chaperones].	4.87699e-06
NZ_CP020352.1\|WP_050820871.1\|2388434_2390354_-\|ATP-dependent-DNA-helicase	gnl\|CDD\|224120	COG1199, DinG, Rad3-related DNA helicases [Transcription / DNA replication, recombination, and repair].	2.85025e-127
NZ_CP020352.1\|WP_025811247.1\|2383936_2384488_-\|GNAT-family-N-acetyltransferase	gnl\|CDD\|224584	COG1670, RimL, Acetyltransferases, including N-acetylases of ribosomal proteins [Translation, ribosomal structure and biogenesis].	2.6944e-14
NZ_CP020352.1\|WP_025811238.1\|2399256_2399763_-\|PTS-glucose-transporter-subunit-IIA	gnl\|CDD\|376324	pfam00358, PTS_EIIA_1, phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 1.	5.41366e-67
NZ_CP020352.1\|WP_025811241.1\|2394375_2394927_-\|DUF1273-domain-containing-protein	gnl\|CDD\|237461	PRK13660, PRK13660, hypothetical protein; Provisional.	1.13882e-100
NZ_CP020352.1\|WP_025811236.1\|2400376_2400820_-\|hypothetical-protein	gnl\|CDD\|372950	pfam14179, YppG, YppG-like protein. The YppG-like protein family includes the B. subtilis YppG protein, which is functionally uncharacterized. This family of proteins is found in bacteria. Proteins in this family are typically between 115 and 181 amino acids in length. There are two completely conserved residues (F and G) that may be functionally important.	9.4554e-18
NZ_CP020352.1\|WP_003182857.1\|2382127_2382223_-\|Fur-regulated-basic-protein-FbpC	gnl\|CDD\|380216	NF033217, Fur_reg_FbpC, Fur-regulated basic protein FbpC. Members of this family are FbpC, Fur-regulated basic protein C. This protein has also been described as MrgC (metal-regulated gene C). Members of this family are found so far only in the genus Bacillus, although the small size may have interferred in gene-finding.	8.14213e-08
NZ_CP020352.1\|WP_035338547.1\|2378419_2381983_-\|dynamin	gnl\|CDD\|206739	cd09912, DLP_2, Dynamin-like protein including dynamins, mitofusins, and guanylate-binding proteins. The dynamin family of large mechanochemical GTPases includes the classical dynamins and dynamin-like proteins (DLPs) that are found throughout the Eukarya. This family also includes bacterial DLPs. These proteins catalyze membrane fission during clathrin-mediated endocytosis. Dynamin consists of five domains; an N-terminal G domain that binds and hydrolyzes GTP, a middle domain (MD) involved in self-assembly and oligomerization, a pleckstrin homology (PH) domain responsible for interactions with the plasma membrane, GED, which is also involved in self-assembly, and a proline arginine rich domain (PRD) that interacts with SH3 domains on accessory proteins. To date, three vertebrate dynamin genes have been identified; dynamin 1, which is brain specific, mediates uptake of synaptic vesicles in presynaptic terminals; dynamin-2 is expressed ubiquitously and similarly participates in membrane fission; mutations in the MD, PH and GED domains of dynamin 2 have been linked to human diseases such as Charcot-Marie-Tooth peripheral neuropathy and rare forms of centronuclear myopathy. Dynamin 3 participates in megakaryocyte progenitor amplification, and is also involved in cytoplasmic enlargement and the formation of the demarcation membrane system. This family also includes mitofusins (MFN1 and MFN2 in mammals) that are involved in mitochondrial fusion. Dynamin oligomerizes into helical structures around the neck of budding vesicles in a GTP hydrolysis-dependent manner.	1.09591e-44
NZ_CP020352.1\|WP_011198064.1\|2400998_2401199_+\|hypothetical-protein	gnl\|CDD\|290882	pfam14178, YppF, YppF-like protein. The YppF-like protein family includes the B. subtilis YppF protein, which is functionally uncharacterized. This family of proteins is found in bacteria. Proteins in this family are approximately 60 amino acids in length. There is a conserved LLDF sequence motif.	8.01632e-25
NZ_CP020352.1\|WP_050820870.1\|2392312_2393464_-\|class-I-SAM-dependent-RNA-methyltransferase	gnl\|CDD\|223194	COG0116, COG0116, Predicted N6-adenine-specific DNA methylase [DNA replication, recombination, and repair].	0
NZ_CP020352.1\|WP_048350161.1\|2384561_2385878_-\|purine-permease	gnl\|CDD\|274468	TIGR03173, pbuX, xanthine permease. All the seed members of this model are observed adjacent to genes for either xanthine phosphoribosyltransferase (for the conversion of xanthine to guanine, GenProp0696) or genes for the conversion of xanthine to urate and its concomitant catabolism (GenProp0640, GenProp0688, GenProp0686 and GenProp0687). A number of sequences scoring higher than trusted to this model are found in different genomic contexts, and the possibility exist that these transport related compounds in addition to or instead of xanthine itself. The outgroup to this family are sequences which are characterized as uracil permeases or are adjacent to established uracil phosphoribosyltransferases.	0

>NZ_CP020352.1|WP_026579404.1|2390836_2391199_-|hypothetical-protein
MNSVSQEIQDFSIQSLESTFNKLTNAYKSTKEKGSNTTLVKKRLNAVKIGLESLKGIWNGEDFGYNEEIILTSKEVLQGIIPSIEKQIAKAKEGSPQKTLKERRLTALKLAIESLENRLI
>NZ_CP020352.1|WP_050820871.1|2388434_2390354_-|ATP-dependent-DNA-helicase
MTASRLPFSLTKTKNFYEELNNWIGDVFYDILPEKGFDLRDEQIFMAFQLERAFKEKKVMFAEAGVGTGKTLVYLLFALSYARYTGKPAVIACADETLIEQLVKKEGDIYKISEHLGLTIDARLSKSHDQYLCLKKLEKTMQRSDDEKWLDLYESLPSFVHESHGMQTFYPYGDRKEYSSLSNDEWSEIGYDSFQDCLTCDMRHRCGLTLSRDHYRKSTDFIICSHDFYMEHVWTEEARKREGQLPLLPEHSAVVFDEGHLLEFAAQKALTYRVKQSTLEMFLERLLGNDIREEFAELVEEALRANDEFFGLLSENAEKAEGSERMRIKDHANLKKAAAELYDLLEKIGEALVFEGELYTIDQYALTVVEEYLEQMAYSLSLYRKNAISWLEQKELESTFVVMPRTVSEVLGEKVFSKQIPFIFSSATLSENGSFDYLATSLGIRDYLSFTVDSPYDYEEQMKVYIHRIEEREAKEKAAIRAIKESGGKTLILFPSFEELLSFKRRTAQESFSFPVYFEGDEEISTIVEKFQTVEESVLCSVHLWEGLDIPGDPLQNVIIWSLPFPPHDPVFEAKRSGLENAFREVDLPYMLLRLRQGIGRLIRSNSDRGTISIFAGDEKEWNVIKEVESVLPVQPVYV
>NZ_CP020352.1|WP_080131430.1|2386816_2388322_-|carboxypeptidase-M32
MMSINTYEEEFLETLKKISRYEQAINVMYWDMRTGAPRKGEESRAEAIGQLSADVFQMRTSPRMKELLDALAEQKQDLPADTQKAVEEAKKEYDLNSKIPEEEFKEYTTLTSKAESVWEKAKKASDFSLFAPYLEKIIDYKKRFISYWGYEGHPYNTLLDQYEPGVTVEKLDGLFAELREAIVPLVQKIAASDQKPDTSFVHERFPKERQRDVCHFFLKELGYDFEAGRLDETVHPFQVTISQGDARVTTAYNENDFRSAVFGTIHECGHAIYEQNIDEALGGTNLADGASMGIHESQSLFYENFIGRHQGFWKRYFEKIKEASPDQFAGVTAEGFYRAVNESKPSLIRIEADELTYPLHIMIRYEIEKAIFSGEVSVGDLPKLWNDKYEAYLGITPPDDAKGILQDVHWSGGDFGYFPSYALGYMYAAQLKSSMQKDFPEFDRLIENGEFQPIRKWLTEHVHIHGKRKKPLDIIKSATGEELNVRHLIRYLKEKYTTLYL
>NZ_CP020352.1|WP_048350162.1|2385874_2386459_-|xanthine-phosphoribosyltransferase
MEKLKRKIAEHGTVLSDEVLKVDSFLNHQIDPELMLAVGEEFASLFREEGVTKIVTIESSGIAPAVMAGLKLGVPVVFARKRQSLTLTENLLTASVYSFTKKTESTIAVSASHLSKDDKVLIIDDFLANGQAAKGLVSIIEQAGAKVCGIGIVIEKSFQTGREELDNLGIRVESLARIASLANGKVTFLQEVGS
>NZ_CP020352.1|WP_048350161.1|2384561_2385878_-|purine-permease
MKLTAGKTVSLSIQHVLAMYAGAVVVPLIVGGALGLTPAELTYLVSADILMCGVATLLQVWKNRFFGIGLPVVLGCTFTAVSPMIAIASKYGIPAIYGSIIAGGVIVVIVSFFFGKLVKFFPPVVTGSVVTVIGITLIPVAMNNMAGGEGSSDFGELSNLALAFSVLLVIVLLYRFTTGFVKSISILIGLIAGTAGAALIGKVDFTEVANAGWVQMIHPFYFGAPVFEVTPILTMSLVLIVSLVESTGVYFALGDLTDRRLTEKDLAKGYRAEGIAVLLGGVFNAFPYTAYSQNVGLVQLTGVKKNSVIALAGAFLILLGLFPKAAALTTIIPKPVLGGAMVAMFGMVIAYGIKMLSRVDFGKQENLLIVACSVGIGLGVTVVPQMFQNLPDAVKLLTENGIVAGSFTAILLNAIFHIHPFSRHAEQPEAVPTEKSAV
>NZ_CP020352.1|WP_025811247.1|2383936_2384488_-|GNAT-family-N-acetyltransferase
METVNELVERISTKDGGEAVIRPVKMADAPDIIEAVKDVIHTGVYIQKERPRTLEEERAFISDMMEKDNMYAAVEIDGAVYGIARVIRGDLKMKRHTGLFRTWLHSKAQGLGIGREIMSYTLNWCRLHRLHKLCLTVFSANQAALSLYEKSGFIIEGVQKEQVLADGNYEDEVLMAYFFERST
>NZ_CP020352.1|WP_048350159.1|2382797_2383895_-|chalcone-synthase
MAYVISAGTSLPRFHVDQKEAAVFAREMFKDSFKDIDRLLAAFQNGEIASRQFVMPLDWYKEARSFKEKNQAYIEMAVRHSAEAVSNCLSDERFLKKEIPFEKIEAIFFISSTGISTPGIEAKLMNILPFSKRMKRIPIWGLGCAGGASGIARAFEYCKAFPKAHVLVIAAELCSLTFQKDDQSKSNLIGTSLFGDGIAALLMCGKEADISSAGLEVLPEVVSSQSATLEDSEDVMGWEINDNGFRVVFSRDIPTLIKTHLKNDVEAFLHKEQIALENIQVFLAHPGGKKVLDAYLESLGFSEAHIDCSRKILARHGNMSSATVMHVLKEYCLHGSPIPGKYGLLGALGPGFSSELLLLKWTKRE
>NZ_CP020352.1|WP_035338546.1|2382286_2382790_-|hypothetical-protein
MFWTILSIFAAQRLFELAVAKFNEKNALKEGAVEYGRRHYPYIVAMHVLFFICLIWETIAFQRGPLPMALVVLTGLIFTQALRYWALFSLGRCWNTKILVIPGTNLVRKGPYQWFKHPNYVAVALEFLLLPLLFQAYATALLFTVLNAAVMYIRIRTEEQALEKLHH
>NZ_CP020352.1|WP_003182857.1|2382127_2382223_-|Fur-regulated-basic-protein-FbpC
MTMFFLLAAVSTYSLLIGYVLKGIAGANPQK
>NZ_CP020352.1|WP_035338547.1|2378419_2381983_-|dynamin
MAQINTKDDLLKRAGAVFGELRKNGDDKRSAQLAGIIRKWLRKEVYIALTGHYSAGKSSLLNALLQEDVLPTSPIPTSANLVLVRRGEMKTTLHTIDGRYARMDGSYDKEKVQAYCRDGSQIEMVEIEGPFSGIAPQAVLIDTPGIDSTDDAHFLSASSILHQADALFYVVHYNHVHSEENVKFLRSIKDQIPNIFFIVNQIDRHDEAETGFHAYKKQVLDMLQKEGIDEDHLFFTSVTEAGHPLNEFTWLRAVLERLQHQSEQQLKAYTEQKISSLIHEHIKMLISDEDTALSEETASLREKVESVKARLADLIEQEKRAEEQIKKDIHTIAQNANLTPFEMRELAKSYLESIEKGFKKGFLFSKAKTLEEKQKRKEVFLADVQKRVQAEIDWHAIEAIKAFMNRFNVQSDELLRDVLQFRTEVDENELLRSQKKGAELTPEYVLNYTKELAENIRRKAKRQAQPLIDQLKTFIKERNGLQAEAMKVEYEAEQAKLAELESRLIEQQKSYEKANRLWDQWEHGDQSELPANWYAEEKKPLEKPLDARRAEPVRVQGENSSRKDDVLHPKKGVSEYIEHFFDLARFLADIPALEKQRKAFLDKTERLHTRQFTLALFGAFSSGKSSFANALCGRKVLPSSPTPTTATINKITKPSGTKRNGTAEVVFKTEEEIRAELDQLLEGKMAAAKGSAFGEKLGKLLKKGDLDDDERLLIKNFLKAYERYGSYINDREILTISAEDLHPYVAEEQTACAVREVTVYLSTPVTEKGITIVDTPGASSMNKRHTELAFQYMKDADALLYLTYYQHAFSKADRSFLRKLGLIKDAFSMDKMFFILNAADLAENPAELQTVEDYVRGELLKEGIQNPHLYHVSSKQELSKKASPNNDFSRLKRELDDFIENGLARTAVEELLDEGKKLCETVFQLRRSLHRSAEEKEAEQKRIAKAYQAALAVIEEAEKGSSILQMTEKDVEEQFYHMRQRLAFYAHDLFKAAIHPGLQNGDWRANLQTALKSCLKEYEFEFLQELKALDIRMENIMLKYAGEQWANAVKERLADNPYFAVHITADRPETGSWETAAASADEGEFADELKMFKTPKAFFQQNGKARLVEAFTAKLSAITDEWIQREKRTCLKRCKTSAGQLQKAAVLSAKAQLEEQKEAYFHEPLESAERENIEKAHNLAGEWLRSS
>NZ_CP020352.1|WP_050820870.1|2392312_2393464_-|class-I-SAM-dependent-RNA-methyltransferase
MKTYTLIATAPMGIEAIVAKEVRDLGYDCTVDNGKVLFEGDALAICRANLWLRTADRVKVQVAEFTAKTFDELFEKTKAIDWSAYLPEHASFPVTGKSVKSVLASVPDCQRIVKKAIAEKLKSAYQTQGGWLEETGPEYKIEVSLLKDKAVITLDSSGAGLHKRGYRTGQGEAPLKETLAAALILLTNWTPDRPFVDPFCGSGTIPIEAALIGQNIAPGFNRDFASERWEWIGKEVWDQARLEVEDKANYDQPLSISGYDLDHRMIEIAKANAEEAGLADFIEFKQMQVKDFRTKEEYGVIVGNPPYGERLGEKKAVEAMYKDMGEAFAGLDTWSVYMLTSNERFEECYGKKATKKRKLFNGFIKTDYYQYWGKRPPKPNKEK
>NZ_CP020352.1|WP_003182873.1|2393998_2394295_-|cell-division-regulator-GpsB
MLADKVKLSAKEILEKEFKTGVRGYKQEDVDKFLDMVIKDYEAFHQEIEELQQENLQLKKQLEEANKRQPAQSNTTNFDILKRLSNLEKHVFGSKLYD
>NZ_CP020352.1|WP_025811241.1|2394375_2394927_-|DUF1273-domain-containing-protein
MKVLAVTGYKPFELGIFKQDDKALFYIKKALENRLTALLEEGLEWILISGQLGTELWAAEVAFSLQEEYPDLKVAVITPFLEQEKKWNEKNQEQYEAVLAASDFTESLTHRPYESPVQFKQKNRFFIEKADGLLILYDEEREGSPVYMLNEAKKAQEKRDFPIYTITMDDLRVTVEECSFYEE
>NZ_CP020352.1|WP_020451911.1|2395043_2395313_-|spore-coat-protein-CotD
MHCKPHVMAPIVHPTQCCYNHTQSKTVVPHIHPQHTTNVNHQHFQHVHYFPQTQSAVNQASHQHFVSPGAGMMPGPGAMPGPGMGMGPY
>NZ_CP020352.1|WP_020451912.1|2395554_2396868_-|nucleic-acid-binding-enzyme-YprB
MSLKRKLNRYKHHLGNQAKEEKTDSPAEKRTSVSVGFEKEWAELGVKPYVFEECYCLIREVVYPLTHVHGHYAFSELHETVRHWNNSAVSHSLSSKGYTPSQLFFFDTETTGLGGGAGNAIFLLGHARVYDDCVVVKQHLLPKPGHEAALYHSFLSEVDIQSLVTYNGKAFDWPQVKTRHTLIRDRVPKLPDFGHFDLLHGSRRLWKHKFDRVSLGTVEKEELGVGRTEDTPGYLAPMMYFHFLKEERPEIIEGVLRHNELDVLSLISLYIHLSKKILAPEQTAEANEKYAMAKWLLANRETELATAQLQELEKKPFEQSERASFDLSMQYKKQGMLKEAASLWLKLLNGKDGKTAWRAGIELAKYLEHRKRDIRAALEVTDNLMLQHGSQKFELTEKEVADLLKRRERLERKFGRNIPRASAENDGLRKPNISSML
>NZ_CP020352.1|WP_025811239.1|2396873_2399153_-|DEAD/DEAH-box-helicase
MTALFQKRNLTDMIQLLKEDQAFSKQIVHWHTIEEKEADYRDFPEGLDKKIVHALQQRGIEKLYSHQADAFLHSAAGKNVTLVTPTASGKTLCYNLPVIQRITEDQDARSLYLFPTKALSQDQKSELNELIDEIGASINCYTYDGDTSPSIRQKVRKAGHIVITNPDMLHSAILPHHTKWVSLFENLKYIVIDELHVYRGVFGSHVANVIRRLMRIAEFYGSKPQFICTSATIANPLELAETLTGQPMHLISNNGAPSGRKHFLFYNPPMVNRSLNIRKSATLEVRKLASEFLKNGIQTIVFARSRVRVEIILTYLQEVMKPIVGPNSIQGYRGGYLPNERRVIEKGLRSGDIKGVVSTNALELGVDIGQLQTCIMTGYPGTISSAWQQAGRAGRRHNEALIVMVASSAPLDQYIVQNPDYFFKQNPETAVINPDNLVILVDHIKCAAFELPFKRTDTFGGAEIEDILDYLAEERVLHLTGNTYHWMNDAFPAHGISLRSASQENVVIIDQSKREKAKVIGEMDRFSAMTLLHDEAIYLHQGIQYQVEKLDWEEKKAFVREVQVDYYTDANLAVLLRVLEVDKEKKTETAAFGFGDVTVQAKATIFKKIKFETHENIGSGPIHLPEEELHTNAAWVQVNDDERQNWNDIKLEEALLGTANVLGHVAALKVMCDPSDLHVFPQIKAVHNEKPTIFLYDRYPGGVGLSQKVYEQMPEIISEAHRLISRCPCESGCPSCIGVGGSGDQTKNNALRLLKGWKG
>NZ_CP020352.1|WP_025811238.1|2399256_2399763_-|PTS-glucose-transporter-subunit-IIA
MLKKLFGIGQKQNEVKEETVFSPVDGQVLELSKVPDPVFSQKMMGDGMAVEPSGGEIVSPVDGEVIQLFHTKHAIGIRSLSGVELLIHIGLETVAMEGVGFTAHVKEGDKVKVGDPLITCDLELIKEKASSTITPIVIMNGDVLERVTKHEEAKAKKSETKLMELKVK
>NZ_CP020352.1|WP_025811237.1|2399938_2400358_+|hypothetical-protein
MDFKENDNHSLSKKDEIQEAVEQLFYNSPFHDILNSFQKLISDQLQNSTISMNIREENEVLHVDIAVPESFQNGDIMIEAKSRYLHVALKEKKENGGSSSFTSLSRTVLLPYPVREETMKTSWNRNILTVSFPEQKKHE
>NZ_CP020352.1|WP_025811236.1|2400376_2400820_-|hypothetical-protein
MEERRTAVYPYSGPGAYVQADQQNHHAYHPVQPYYSQETVPYAHPAQRPQAYQPIEQNVDPFSVYHQPVQQQMQPPLPPFQAFPYQHPYPSPRPNLHQPSQFQSFLSQFKKKNGQFDFSKMMDTAGQMVSTVNQVGALAKGFMGFFK
>NZ_CP020352.1|WP_011198064.1|2400998_2401199_+|hypothetical-protein
MNVSYLRNCFAEMKQYETDCMNKLMDFAKFLYIQGHLTLNEFRTSMKVLEANGAQHPAYDMNTDAS

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Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity
NZ_CP020352_2	2.6\|878307\|20\|NZ_CP020352\|CRT	878307-878326	20	NZ_CP020352.1	873127-873146	1	0.95
NZ_CP020352_2	2.6\|878307\|20\|NZ_CP020352\|CRT	878307-878326	20	NZ_CP020352.1	873649-873668	1	0.95
NZ_CP020352_2	2.6\|878307\|20\|NZ_CP020352\|CRT	878307-878326	20	NZ_CP020352.1	874477-874496	1	0.95
NZ_CP020352_2	2.6\|878307\|20\|NZ_CP020352\|CRT	878307-878326	20	NZ_CP020352.1	875188-875207	1	0.95
NZ_CP020352_2	2.6\|878307\|20\|NZ_CP020352\|CRT	878307-878326	20	NZ_CP020352.1	875485-875504	1	0.95
NZ_CP020352_2	2.6\|878307\|20\|NZ_CP020352\|CRT	878307-878326	20	NZ_CP020352.1	876016-876035	1	0.95
NZ_CP020352_2	2.6\|878307\|20\|NZ_CP020352\|CRT	878307-878326	20	NZ_CP020352.1	876070-876089	1	0.95
NZ_CP020352_2	2.6\|878307\|20\|NZ_CP020352\|CRT	878307-878326	20	NZ_CP020352.1	876124-876143	1	0.95
NZ_CP020352_2	2.14\|878820\|29\|NZ_CP020352\|CRT	878820-878848	29	NZ_CP020352.1	873469-873497	1	0.966
NZ_CP020352_2	2.14\|878820\|29\|NZ_CP020352\|CRT	878820-878848	29	NZ_CP020352.1	874000-874028	1	0.966
NZ_CP020352_2	2.14\|878820\|29\|NZ_CP020352\|CRT	878820-878848	29	NZ_CP020352.1	874720-874748	1	0.966
NZ_CP020352_2	2.1\|878001\|20\|NZ_CP020352\|CRT	878001-878020	20	NZ_CP020352.1	876736-876755	2	0.9
NZ_CP020352_2	2.6\|878307\|20\|NZ_CP020352\|CRT	878307-878326	20	NZ_CP020352.1	872659-872678	2	0.9
NZ_CP020352_2	2.6\|878307\|20\|NZ_CP020352\|CRT	878307-878326	20	NZ_CP020352.1	873541-873560	2	0.9
NZ_CP020352_2	2.6\|878307\|20\|NZ_CP020352\|CRT	878307-878326	20	NZ_CP020352.1	873766-873785	2	0.9
NZ_CP020352_2	2.6\|878307\|20\|NZ_CP020352\|CRT	878307-878326	20	NZ_CP020352.1	874072-874091	2	0.9
NZ_CP020352_2	2.6\|878307\|20\|NZ_CP020352\|CRT	878307-878326	20	NZ_CP020352.1	874594-874613	2	0.9
NZ_CP020352_2	2.6\|878307\|20\|NZ_CP020352\|CRT	878307-878326	20	NZ_CP020352.1	874792-874811	2	0.9
NZ_CP020352_2	2.6\|878307\|20\|NZ_CP020352\|CRT	878307-878326	20	NZ_CP020352.1	875647-875666	2	0.9
NZ_CP020352_2	2.6\|878307\|20\|NZ_CP020352\|CRT	878307-878326	20	NZ_CP020352.1	875728-875747	2	0.9
NZ_CP020352_2	2.13\|878766\|29\|NZ_CP020352\|CRT	878766-878794	29	NZ_CP020352.1	873469-873497	2	0.931
NZ_CP020352_2	2.13\|878766\|29\|NZ_CP020352\|CRT	878766-878794	29	NZ_CP020352.1	874000-874028	2	0.931
NZ_CP020352_2	2.13\|878766\|29\|NZ_CP020352\|CRT	878766-878794	29	NZ_CP020352.1	874720-874748	2	0.931
NZ_CP020352_2	2.13\|878766\|29\|NZ_CP020352\|CRT	878766-878794	29	NZ_CP020352.1	875305-875333	2	0.931
NZ_CP020352_2	2.14\|878820\|29\|NZ_CP020352\|CRT	878820-878848	29	NZ_CP020352.1	875305-875333	2	0.931
NZ_CP020352_2	2.23\|879495\|29\|NZ_CP020352\|CRT	879495-879523	29	NZ_CP020352.1	879585-879613	2	0.931

1. spacer 2.6|878307|20|NZ_CP020352|CRT matches to position: 873127-873146, mismatch: 1, identity: 0.95

agcaaccggcccgacgggag	CRISPR spacer
agcaaccggtccgacgggag	Protospacer
*********.**********

2. spacer 2.6|878307|20|NZ_CP020352|CRT matches to position: 873649-873668, mismatch: 1, identity: 0.95

agcaaccggcccgacgggag	CRISPR spacer
agcaaccggtccgacgggag	Protospacer
*********.**********

3. spacer 2.6|878307|20|NZ_CP020352|CRT matches to position: 874477-874496, mismatch: 1, identity: 0.95

agcaaccggcccgacgggag	CRISPR spacer
agcaaccggtccgacgggag	Protospacer
*********.**********

4. spacer 2.6|878307|20|NZ_CP020352|CRT matches to position: 875188-875207, mismatch: 1, identity: 0.95

agcaaccggcccgacgggag	CRISPR spacer
agcaaccggtccgacgggag	Protospacer
*********.**********

5. spacer 2.6|878307|20|NZ_CP020352|CRT matches to position: 875485-875504, mismatch: 1, identity: 0.95

agcaaccggcccgacgggag	CRISPR spacer
agcaaccggtccgacgggag	Protospacer
*********.**********

6. spacer 2.6|878307|20|NZ_CP020352|CRT matches to position: 876016-876035, mismatch: 1, identity: 0.95

agcaaccggcccgacgggag	CRISPR spacer
agcaaccggcccgacaggag	Protospacer
***************.****

7. spacer 2.6|878307|20|NZ_CP020352|CRT matches to position: 876070-876089, mismatch: 1, identity: 0.95

agcaaccggcccgacgggag	CRISPR spacer
agcaaccggcccgacaggag	Protospacer
***************.****

8. spacer 2.6|878307|20|NZ_CP020352|CRT matches to position: 876124-876143, mismatch: 1, identity: 0.95

agcaaccggcccgacgggag	CRISPR spacer
agcaaccggcccgacaggag	Protospacer
***************.****

9. spacer 2.14|878820|29|NZ_CP020352|CRT matches to position: 873469-873497, mismatch: 1, identity: 0.966

agcgacaggcgcaacgggagcgacaggcg	CRISPR spacer
agccacaggcgcaacgggagcgacaggcg	Protospacer
*** *************************

10. spacer 2.14|878820|29|NZ_CP020352|CRT matches to position: 874000-874028, mismatch: 1, identity: 0.966

agcgacaggcgcaacgggagcgacaggcg	CRISPR spacer
agccacaggcgcaacgggagcgacaggcg	Protospacer
*** *************************

11. spacer 2.14|878820|29|NZ_CP020352|CRT matches to position: 874720-874748, mismatch: 1, identity: 0.966

agcgacaggcgcaacgggagcgacaggcg	CRISPR spacer
agccacaggcgcaacgggagcgacaggcg	Protospacer
*** *************************

12. spacer 2.1|878001|20|NZ_CP020352|CRT matches to position: 876736-876755, mismatch: 2, identity: 0.9

tccaacaggtccaaccggcc	CRISPR spacer
tccaacaggagcaaccggcc	Protospacer
*********  *********

13. spacer 2.6|878307|20|NZ_CP020352|CRT matches to position: 872659-872678, mismatch: 2, identity: 0.9

agcaaccggcccgacgggag	CRISPR spacer
agcaaccggcgcaacgggag	Protospacer
********** *.*******

14. spacer 2.6|878307|20|NZ_CP020352|CRT matches to position: 873541-873560, mismatch: 2, identity: 0.9

agcaaccggcccgacgggag	CRISPR spacer
agcaaccggcgcaacgggag	Protospacer
********** *.*******

15. spacer 2.6|878307|20|NZ_CP020352|CRT matches to position: 873766-873785, mismatch: 2, identity: 0.9

agcaaccggcccgacgggag	CRISPR spacer
agcaaccggagcgacgggag	Protospacer
*********  *********

16. spacer 2.6|878307|20|NZ_CP020352|CRT matches to position: 874072-874091, mismatch: 2, identity: 0.9

agcaaccggcccgacgggag	CRISPR spacer
agcaaccggcgcaacgggag	Protospacer
********** *.*******

17. spacer 2.6|878307|20|NZ_CP020352|CRT matches to position: 874594-874613, mismatch: 2, identity: 0.9

agcaaccggcccgacgggag	CRISPR spacer
agcaaccggagcgacgggag	Protospacer
*********  *********

18. spacer 2.6|878307|20|NZ_CP020352|CRT matches to position: 874792-874811, mismatch: 2, identity: 0.9

agcaaccggcccgacgggag	CRISPR spacer
agcaaccggcgcaacgggag	Protospacer
********** *.*******

19. spacer 2.6|878307|20|NZ_CP020352|CRT matches to position: 875647-875666, mismatch: 2, identity: 0.9

agcaaccggcccgacgggag	CRISPR spacer
agcgaccggaccgacgggag	Protospacer
***.***** **********

20. spacer 2.6|878307|20|NZ_CP020352|CRT matches to position: 875728-875747, mismatch: 2, identity: 0.9

agcaaccggcccgacgggag	CRISPR spacer
agccaccggcccgaccggag	Protospacer
*** *********** ****

21. spacer 2.13|878766|29|NZ_CP020352|CRT matches to position: 873469-873497, mismatch: 2, identity: 0.931

agcgacaggcgcaaccggagcgacaggcg	CRISPR spacer
agccacaggcgcaacgggagcgacaggcg	Protospacer
*** *********** *************

22. spacer 2.13|878766|29|NZ_CP020352|CRT matches to position: 874000-874028, mismatch: 2, identity: 0.931

agcgacaggcgcaaccggagcgacaggcg	CRISPR spacer
agccacaggcgcaacgggagcgacaggcg	Protospacer
*** *********** *************

23. spacer 2.13|878766|29|NZ_CP020352|CRT matches to position: 874720-874748, mismatch: 2, identity: 0.931

agcgacaggcgcaaccggagcgacaggcg	CRISPR spacer
agccacaggcgcaacgggagcgacaggcg	Protospacer
*** *********** *************

24. spacer 2.13|878766|29|NZ_CP020352|CRT matches to position: 875305-875333, mismatch: 2, identity: 0.931

agcgacaggcgcaaccggagcgacaggcg	CRISPR spacer
agcgacaggcccaacaggagcgacaggcg	Protospacer
********** **** *************

25. spacer 2.14|878820|29|NZ_CP020352|CRT matches to position: 875305-875333, mismatch: 2, identity: 0.931

agcgacaggcgcaacgggagcgacaggcg	CRISPR spacer
agcgacaggcccaacaggagcgacaggcg	Protospacer
********** ****.*************

26. spacer 2.23|879495|29|NZ_CP020352|CRT matches to position: 879585-879613, mismatch: 2, identity: 0.931

cccgacaggtccaactggcccgacaggtc	CRISPR spacer
cccgacaggtccaaccggcccaacaggtc	Protospacer
***************.*****.*******

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity
NZ_CP020352_2	2.4\|878190\|29\|NZ_CP020352\|CRT	878190-878218	29	NZ_CP009336	Bacillus thuringiensis strain HD1011 plasmid 1, complete sequence	72681-72709	2	0.931
NZ_CP020352_2	2.13\|878766\|29\|NZ_CP020352\|CRT	878766-878794	29	NZ_AP022593	Mycolicibacterium arabiense strain JCM 18538 plasmid pJCM18538, complete sequence	3051385-3051413	2	0.931
NZ_CP020352_2	2.13\|878766\|29\|NZ_CP020352\|CRT	878766-878794	29	NC_021304	Streptomyces phage Sujidade, complete genome	17743-17771	2	0.931
NZ_CP020352_2	2.12\|878712\|29\|NZ_CP020352\|CRT	878712-878740	29	NZ_AP022593	Mycolicibacterium arabiense strain JCM 18538 plasmid pJCM18538, complete sequence	3051385-3051413	3	0.897
NZ_CP020352_2	2.12\|878712\|29\|NZ_CP020352\|CRT	878712-878740	29	NC_021304	Streptomyces phage Sujidade, complete genome	17743-17771	3	0.897
NZ_CP020352_2	2.23\|879495\|29\|NZ_CP020352\|CRT	879495-879523	29	NC_019718	Enterobacteria phage vB_EcoS_Rogue1, complete genome	10355-10383	3	0.897
NZ_CP020352_2	2.4\|878190\|29\|NZ_CP020352\|CRT	878190-878218	29	NC_010180	Bacillus mycoides KBAB4 plasmid pBWB401, complete sequence	116700-116728	4	0.862
NZ_CP020352_2	2.12\|878712\|29\|NZ_CP020352\|CRT	878712-878740	29	NZ_AP022593	Mycolicibacterium arabiense strain JCM 18538 plasmid pJCM18538, complete sequence	3051349-3051377	4	0.862
NZ_CP020352_2	2.13\|878766\|29\|NZ_CP020352\|CRT	878766-878794	29	NZ_AP022593	Mycolicibacterium arabiense strain JCM 18538 plasmid pJCM18538, complete sequence	3051349-3051377	4	0.862
NZ_CP020352_2	2.13\|878766\|29\|NZ_CP020352\|CRT	878766-878794	29	NC_048029	Sphingomonas phage Scott isolate SPS, complete genome	4951-4979	4	0.862
NZ_CP020352_2	2.13\|878766\|29\|NZ_CP020352\|CRT	878766-878794	29	NC_048029	Sphingomonas phage Scott isolate SPS, complete genome	4969-4997	4	0.862
NZ_CP020352_2	2.14\|878820\|29\|NZ_CP020352\|CRT	878820-878848	29	NZ_CP033971	Cupriavidus pauculus strain FDAARGOS_614 plasmid unnamed2, complete sequence	251784-251812	4	0.862
NZ_CP020352_2	2.20\|879279\|38\|NZ_CP020352\|CRT	879279-879316	38	CP011076	Brevibacillus laterosporus strain B9 plasmid unnamed2, complete sequence	251919-251956	4	0.895
NZ_CP020352_2	2.23\|879495\|29\|NZ_CP020352\|CRT	879495-879523	29	MF158039	Shigella phage Sf12, complete genome	35337-35365	4	0.862
NZ_CP020352_2	2.23\|879495\|29\|NZ_CP020352\|CRT	879495-879523	29	MF158042	Shigella phage Sd1, complete genome	31393-31421	4	0.862
NZ_CP020352_2	2.4\|878190\|29\|NZ_CP020352\|CRT	878190-878218	29	NZ_AP014705	Methylobacterium aquaticum strain MA-22A plasmid pMaq22A_1p, complete sequence	515451-515479	5	0.828
NZ_CP020352_2	2.12\|878712\|29\|NZ_CP020352\|CRT	878712-878740	29	NC_048029	Sphingomonas phage Scott isolate SPS, complete genome	4951-4979	5	0.828
NZ_CP020352_2	2.12\|878712\|29\|NZ_CP020352\|CRT	878712-878740	29	NC_048029	Sphingomonas phage Scott isolate SPS, complete genome	4969-4997	5	0.828
NZ_CP020352_2	2.14\|878820\|29\|NZ_CP020352\|CRT	878820-878848	29	NZ_CP009336	Bacillus thuringiensis strain HD1011 plasmid 1, complete sequence	72411-72439	5	0.828
NZ_CP020352_2	2.23\|879495\|29\|NZ_CP020352\|CRT	879495-879523	29	NZ_CP009336	Bacillus thuringiensis strain HD1011 plasmid 1, complete sequence	71449-71477	5	0.828
NZ_CP020352_2	2.24\|879549\|29\|NZ_CP020352\|CRT	879549-879577	29	MF158039	Shigella phage Sf12, complete genome	35337-35365	5	0.828
NZ_CP020352_2	2.24\|879549\|29\|NZ_CP020352\|CRT	879549-879577	29	MF158042	Shigella phage Sd1, complete genome	31393-31421	5	0.828
NZ_CP020352_2	2.4\|878190\|29\|NZ_CP020352\|CRT	878190-878218	29	NZ_CP009336	Bacillus thuringiensis strain HD1011 plasmid 1, complete sequence	73702-73730	6	0.793
NZ_CP020352_2	2.4\|878190\|29\|NZ_CP020352\|CRT	878190-878218	29	NZ_CP053933	Bacillus thuringiensis strain FDAARGOS_794 plasmid unnamed2, complete sequence	41402-41430	6	0.793
NZ_CP020352_2	2.4\|878190\|29\|NZ_CP020352\|CRT	878190-878218	29	NZ_CP053936	Bacillus thuringiensis strain FDAARGOS_794 plasmid unnamed4, complete sequence	11750-11778	6	0.793
NZ_CP020352_2	2.4\|878190\|29\|NZ_CP020352\|CRT	878190-878218	29	NZ_CP009718	Bacillus thuringiensis strain HD682 plasmid pBGN_2, complete sequence	15394-15422	6	0.793
NZ_CP020352_2	2.4\|878190\|29\|NZ_CP020352\|CRT	878190-878218	29	NZ_CP015063	Mesorhizobium ciceri strain CC1192 plasmid pMc1192, complete sequence	545-573	6	0.793
NZ_CP020352_2	2.4\|878190\|29\|NZ_CP020352\|CRT	878190-878218	29	NZ_CP015063	Mesorhizobium ciceri strain CC1192 plasmid pMc1192, complete sequence	1166-1194	6	0.793
NZ_CP020352_2	2.4\|878190\|29\|NZ_CP020352\|CRT	878190-878218	29	NZ_CP015063	Mesorhizobium ciceri strain CC1192 plasmid pMc1192, complete sequence	648064-648092	6	0.793
NZ_CP020352_2	2.12\|878712\|29\|NZ_CP020352\|CRT	878712-878740	29	NZ_CP011054	Halomonas sp. KO116 plasmid unnamed2, complete sequence	48395-48423	6	0.793
NZ_CP020352_2	2.13\|878766\|29\|NZ_CP020352\|CRT	878766-878794	29	NZ_CP011054	Halomonas sp. KO116 plasmid unnamed2, complete sequence	48395-48423	6	0.793
NZ_CP020352_2	2.14\|878820\|29\|NZ_CP020352\|CRT	878820-878848	29	NZ_CP016082	Streptomyces sp. SAT1 plasmid unnamed2, complete sequence	220862-220890	6	0.793
NZ_CP020352_2	2.14\|878820\|29\|NZ_CP020352\|CRT	878820-878848	29	NZ_CP032340	Azospirillum brasilense strain MTCC4038 plasmid p1, complete sequence	1460513-1460541	6	0.793
NZ_CP020352_2	2.14\|878820\|29\|NZ_CP020352\|CRT	878820-878848	29	NZ_CP012915	Azospirillum brasilense strain Sp 7 plasmid ABSP7_p1, complete sequence	671859-671887	6	0.793
NZ_CP020352_2	2.23\|879495\|29\|NZ_CP020352\|CRT	879495-879523	29	CP024687	Bacillus wiedmannii bv. thuringiensis strain FCC41 plasmid pFCC41-3-257K, complete sequence	236390-236418	6	0.793
NZ_CP020352_2	2.23\|879495\|29\|NZ_CP020352\|CRT	879495-879523	29	NZ_MF600313	Mycolicibacterium sp. CBMA 213 plasmid pCBMA213_1, complete sequence	135804-135832	6	0.793
NZ_CP020352_2	2.23\|879495\|29\|NZ_CP020352\|CRT	879495-879523	29	NZ_CP013556	Rhizobium phaseoli strain N931 plasmid pRphaN931d, complete sequence	1039789-1039817	6	0.793
NZ_CP020352_2	2.23\|879495\|29\|NZ_CP020352\|CRT	879495-879523	29	NZ_CP013567	Rhizobium phaseoli strain N831 plasmid pRphaN831d, complete sequence	1039789-1039817	6	0.793
NZ_CP020352_2	2.4\|878190\|29\|NZ_CP020352\|CRT	878190-878218	29	NZ_CP043441	Cupriavidus campinensis strain MJ1 plasmid unnamed1, complete sequence	1423777-1423805	7	0.759
NZ_CP020352_2	2.11\|878649\|38\|NZ_CP020352\|CRT	878649-878686	38	CP024687	Bacillus wiedmannii bv. thuringiensis strain FCC41 plasmid pFCC41-3-257K, complete sequence	235991-236028	7	0.816
NZ_CP020352_2	2.12\|878712\|29\|NZ_CP020352\|CRT	878712-878740	29	NZ_CP033971	Cupriavidus pauculus strain FDAARGOS_614 plasmid unnamed2, complete sequence	251658-251686	7	0.759
NZ_CP020352_2	2.13\|878766\|29\|NZ_CP020352\|CRT	878766-878794	29	NZ_CP033971	Cupriavidus pauculus strain FDAARGOS_614 plasmid unnamed2, complete sequence	251658-251686	7	0.759
NZ_CP020352_2	2.14\|878820\|29\|NZ_CP020352\|CRT	878820-878848	29	NZ_CP009336	Bacillus thuringiensis strain HD1011 plasmid 1, complete sequence	71380-71408	7	0.759
NZ_CP020352_2	2.14\|878820\|29\|NZ_CP020352\|CRT	878820-878848	29	NZ_CP009336	Bacillus thuringiensis strain HD1011 plasmid 1, complete sequence	72483-72511	7	0.759
NZ_CP020352_2	2.23\|879495\|29\|NZ_CP020352\|CRT	879495-879523	29	HQ632825	Prochlorococcus phage P-SSM5 genomic sequence	61495-61523	7	0.759
NZ_CP020352_2	2.23\|879495\|29\|NZ_CP020352\|CRT	879495-879523	29	HQ632825	Prochlorococcus phage P-SSM5 genomic sequence	65835-65863	7	0.759
NZ_CP020352_2	2.23\|879495\|29\|NZ_CP020352\|CRT	879495-879523	29	AY939844	Prochlorococcus phage P-SSM2, complete genome	21119-21147	7	0.759
NZ_CP020352_2	2.23\|879495\|29\|NZ_CP020352\|CRT	879495-879523	29	AY939844	Prochlorococcus phage P-SSM2, complete genome	25459-25487	7	0.759
NZ_CP020352_2	2.23\|879495\|29\|NZ_CP020352\|CRT	879495-879523	29	CP024687	Bacillus wiedmannii bv. thuringiensis strain FCC41 plasmid pFCC41-3-257K, complete sequence	234925-234953	7	0.759
NZ_CP020352_2	2.23\|879495\|29\|NZ_CP020352\|CRT	879495-879523	29	NZ_CP012101	Bacillus thuringiensis strain HS18-1 plasmid pHS18-2, complete sequence	131426-131454	7	0.759
NZ_CP020352_2	2.23\|879495\|29\|NZ_CP020352\|CRT	879495-879523	29	NZ_CP012101	Bacillus thuringiensis strain HS18-1 plasmid pHS18-2, complete sequence	132887-132915	7	0.759
NZ_CP020352_2	2.23\|879495\|29\|NZ_CP020352\|CRT	879495-879523	29	NZ_CP012101	Bacillus thuringiensis strain HS18-1 plasmid pHS18-2, complete sequence	253740-253768	7	0.759
NZ_CP020352_2	2.23\|879495\|29\|NZ_CP020352\|CRT	879495-879523	29	NZ_CP012105	Bacillus thuringiensis strain HS18-1 plasmid pHS18-6, complete sequence	6689-6717	7	0.759
NZ_CP020352_2	2.23\|879495\|29\|NZ_CP020352\|CRT	879495-879523	29	MN693383	Marine virus AFVG_25M295, complete genome	19328-19356	7	0.759
NZ_CP020352_2	2.23\|879495\|29\|NZ_CP020352\|CRT	879495-879523	29	MN693263	Marine virus AFVG_25M296, complete genome	13978-14006	7	0.759
NZ_CP020352_2	2.24\|879549\|29\|NZ_CP020352\|CRT	879549-879577	29	CP024687	Bacillus wiedmannii bv. thuringiensis strain FCC41 plasmid pFCC41-3-257K, complete sequence	236390-236418	7	0.759
NZ_CP020352_2	2.5\|878244\|38\|NZ_CP020352\|CRT	878244-878281	38	MN694114	Marine virus AFVG_250M252, complete genome	19685-19722	8	0.789
NZ_CP020352_2	2.19\|879207\|47\|NZ_CP020352\|CRT	879207-879253	47	MN694114	Marine virus AFVG_250M252, complete genome	19685-19731	8	0.83
NZ_CP020352_2	2.20\|879279\|38\|NZ_CP020352\|CRT	879279-879316	38	KY421186	Flavobacterium phage FL-1, complete genome	26874-26911	9	0.763
NZ_CP020352_2	2.5\|878244\|38\|NZ_CP020352\|CRT	878244-878281	38	NZ_CP011054	Halomonas sp. KO116 plasmid unnamed2, complete sequence	48386-48423	10	0.737
NZ_CP020352_2	2.20\|879279\|38\|NZ_CP020352\|CRT	879279-879316	38	CP011076	Brevibacillus laterosporus strain B9 plasmid unnamed2, complete sequence	252018-252055	11	0.711
NZ_CP020352_2	2.3\|878118\|47\|NZ_CP020352\|CRT	878118-878164	47	MN694114	Marine virus AFVG_250M252, complete genome	19685-19731	12	0.745

1. spacer 2.4|878190|29|NZ_CP020352|CRT matches to NZ_CP009336 (Bacillus thuringiensis strain HD1011 plasmid 1, complete sequence) position: , mismatch: 2, identity: 0.931

tccaaccggtccgacgggagcaaccggtc	CRISPR spacer
tccaaccggtccgactggagcaacgggtc	Protospacer
*************** ******** ****

2. spacer 2.13|878766|29|NZ_CP020352|CRT matches to NZ_AP022593 (Mycolicibacterium arabiense strain JCM 18538 plasmid pJCM18538, complete sequence) position: , mismatch: 2, identity: 0.931

agcgacaggcgcaaccggagcgacaggcg	CRISPR spacer
agcgaccggcgcaaccggagcgactggcg	Protospacer
****** ***************** ****

3. spacer 2.13|878766|29|NZ_CP020352|CRT matches to NC_021304 (Streptomyces phage Sujidade, complete genome) position: , mismatch: 2, identity: 0.931

agcgacaggcgcaaccggagcgacaggcg	CRISPR spacer
agcgaccggcgcaaccggagcgaccggcg	Protospacer
****** ***************** ****

4. spacer 2.12|878712|29|NZ_CP020352|CRT matches to NZ_AP022593 (Mycolicibacterium arabiense strain JCM 18538 plasmid pJCM18538, complete sequence) position: , mismatch: 3, identity: 0.897

agcgacaggcgcaaccggagcgacaggag	CRISPR spacer
agcgaccggcgcaaccggagcgactggcg	Protospacer
****** ***************** ** *

5. spacer 2.12|878712|29|NZ_CP020352|CRT matches to NC_021304 (Streptomyces phage Sujidade, complete genome) position: , mismatch: 3, identity: 0.897

agcgacaggcgcaaccggagcgacaggag	CRISPR spacer
agcgaccggcgcaaccggagcgaccggcg	Protospacer
****** ***************** ** *

6. spacer 2.23|879495|29|NZ_CP020352|CRT matches to NC_019718 (Enterobacteria phage vB_EcoS_Rogue1, complete genome) position: , mismatch: 3, identity: 0.897

cccgacaggtccaactggcccgacaggtc	CRISPR spacer
tccaacaggtccaactggcccggcaggtc	Protospacer
.**.******************.******

7. spacer 2.4|878190|29|NZ_CP020352|CRT matches to NC_010180 (Bacillus mycoides KBAB4 plasmid pBWB401, complete sequence) position: , mismatch: 4, identity: 0.862

tccaaccggtccgacgggagcaaccggtc	CRISPR spacer
cccaaccggtccaacgggagcaacaggta	Protospacer
.***********.*********** ***

8. spacer 2.12|878712|29|NZ_CP020352|CRT matches to NZ_AP022593 (Mycolicibacterium arabiense strain JCM 18538 plasmid pJCM18538, complete sequence) position: , mismatch: 4, identity: 0.862

agcgacaggcgcaaccggagcgacaggag	CRISPR spacer
agcgaccggcgcaaccggagcgaccggta	Protospacer
****** ***************** ** .

9. spacer 2.13|878766|29|NZ_CP020352|CRT matches to NZ_AP022593 (Mycolicibacterium arabiense strain JCM 18538 plasmid pJCM18538, complete sequence) position: , mismatch: 4, identity: 0.862

agcgacaggcgcaaccggagcgacaggcg	CRISPR spacer
agcgaccggcgcaaccggagcgaccggta	Protospacer
****** ***************** **..

10. spacer 2.13|878766|29|NZ_CP020352|CRT matches to NC_048029 (Sphingomonas phage Scott isolate SPS, complete genome) position: , mismatch: 4, identity: 0.862

agcgacaggcgcaaccggagcgacaggcg	CRISPR spacer
ctcgacaggctcgaccggagcgacaggcg	Protospacer
  ******** *.****************

11. spacer 2.13|878766|29|NZ_CP020352|CRT matches to NC_048029 (Sphingomonas phage Scott isolate SPS, complete genome) position: , mismatch: 4, identity: 0.862

agcgacaggcgcaaccggagcgacaggcg	CRISPR spacer
agcgacaggcgcagccggagcggcaaccg	Protospacer
*************.********.**. **

12. spacer 2.14|878820|29|NZ_CP020352|CRT matches to NZ_CP033971 (Cupriavidus pauculus strain FDAARGOS_614 plasmid unnamed2, complete sequence) position: , mismatch: 4, identity: 0.862

agcgacaggcgcaacgggagcgacaggcg	CRISPR spacer
cgcgacaggcgcaacgggcgcgaccggtg	Protospacer
 ***************** ***** **.*

13. spacer 2.20|879279|38|NZ_CP020352|CRT matches to CP011076 (Brevibacillus laterosporus strain B9 plasmid unnamed2, complete sequence) position: , mismatch: 4, identity: 0.895

agcaaccggggcaaccggagcaacgggagcaaccggcc	CRISPR spacer
agcaaccggggcaaccggagcaaccggagcaactggag	Protospacer
************************ ********.**

14. spacer 2.23|879495|29|NZ_CP020352|CRT matches to MF158039 (Shigella phage Sf12, complete genome) position: , mismatch: 4, identity: 0.862

cccgacaggtccaactggcccgacaggtc	CRISPR spacer
tccagcaggcccaactggcccgacaggtc	Protospacer
.**..****.*******************

15. spacer 2.23|879495|29|NZ_CP020352|CRT matches to MF158042 (Shigella phage Sd1, complete genome) position: , mismatch: 4, identity: 0.862

cccgacaggtccaactggcccgacaggtc	CRISPR spacer
tccagcaggcccaactggcccgacaggtc	Protospacer
.**..****.*******************

16. spacer 2.4|878190|29|NZ_CP020352|CRT matches to NZ_AP014705 (Methylobacterium aquaticum strain MA-22A plasmid pMaq22A_1p, complete sequence) position: , mismatch: 5, identity: 0.828

tccaaccggtccgacgggagcaaccggtc	CRISPR spacer
ggccaccggtccgacgggcgcgaccggtc	Protospacer
  * ************** **.*******

17. spacer 2.12|878712|29|NZ_CP020352|CRT matches to NC_048029 (Sphingomonas phage Scott isolate SPS, complete genome) position: , mismatch: 5, identity: 0.828

agcgacaggcgcaaccggagcgacaggag	CRISPR spacer
ctcgacaggctcgaccggagcgacaggcg	Protospacer
  ******** *.************** *

18. spacer 2.12|878712|29|NZ_CP020352|CRT matches to NC_048029 (Sphingomonas phage Scott isolate SPS, complete genome) position: , mismatch: 5, identity: 0.828

agcgacaggcgcaaccggagcgacaggag	CRISPR spacer
agcgacaggcgcagccggagcggcaaccg	Protospacer
*************.********.**.  *

19. spacer 2.14|878820|29|NZ_CP020352|CRT matches to NZ_CP009336 (Bacillus thuringiensis strain HD1011 plasmid 1, complete sequence) position: , mismatch: 5, identity: 0.828

agcgacaggcgcaacgggagcgacaggcg	CRISPR spacer
tgagacaggagcaacgggaacgacaggag	Protospacer
 * ****** *********.******* *

20. spacer 2.23|879495|29|NZ_CP020352|CRT matches to NZ_CP009336 (Bacillus thuringiensis strain HD1011 plasmid 1, complete sequence) position: , mismatch: 5, identity: 0.828

cccgacaggtccaactggcccgacaggtc	CRISPR spacer
tccaacaggtccaactggccctacaggag	Protospacer
.**.***************** *****

21. spacer 2.24|879549|29|NZ_CP020352|CRT matches to MF158039 (Shigella phage Sf12, complete genome) position: , mismatch: 5, identity: 0.828

cccgacgggtccaactggcccgacaggtc	CRISPR spacer
tccagcaggcccaactggcccgacaggtc	Protospacer
.**..*.**.*******************

22. spacer 2.24|879549|29|NZ_CP020352|CRT matches to MF158042 (Shigella phage Sd1, complete genome) position: , mismatch: 5, identity: 0.828

cccgacgggtccaactggcccgacaggtc	CRISPR spacer
tccagcaggcccaactggcccgacaggtc	Protospacer
.**..*.**.*******************

23. spacer 2.4|878190|29|NZ_CP020352|CRT matches to NZ_CP009336 (Bacillus thuringiensis strain HD1011 plasmid 1, complete sequence) position: , mismatch: 6, identity: 0.793

tccaaccggtccgacgggagcaaccggtc	CRISPR spacer
ggtaaccggcccaacgggagcaaccggcc	Protospacer
  .******.**.**************.*

24. spacer 2.4|878190|29|NZ_CP020352|CRT matches to NZ_CP053933 (Bacillus thuringiensis strain FDAARGOS_794 plasmid unnamed2, complete sequence) position: , mismatch: 6, identity: 0.793

tccaaccggtccgacgggagcaaccggtc	CRISPR spacer
tgtaaccggaccgacgggagcaacaggaa	Protospacer
* .****** ************** **

25. spacer 2.4|878190|29|NZ_CP020352|CRT matches to NZ_CP053936 (Bacillus thuringiensis strain FDAARGOS_794 plasmid unnamed4, complete sequence) position: , mismatch: 6, identity: 0.793

tccaaccggtccgacgggagcaaccggtc	CRISPR spacer
tgtaaccggaccgacgggagcaacaggaa	Protospacer
* .****** ************** **

26. spacer 2.4|878190|29|NZ_CP020352|CRT matches to NZ_CP009718 (Bacillus thuringiensis strain HD682 plasmid pBGN_2, complete sequence) position: , mismatch: 6, identity: 0.793

tccaaccggtccgacgggagcaaccggtc	CRISPR spacer
tgtaaccggaccgacgggagcaacaggaa	Protospacer
* .****** ************** **

27. spacer 2.4|878190|29|NZ_CP020352|CRT matches to NZ_CP015063 (Mesorhizobium ciceri strain CC1192 plasmid pMc1192, complete sequence) position: , mismatch: 6, identity: 0.793

tccaaccggtccgacgggagcaaccggtc	CRISPR spacer
tgcgaccggttcgactggagcaaccggct	Protospacer
* *.******.**** ***********..

28. spacer 2.4|878190|29|NZ_CP020352|CRT matches to NZ_CP015063 (Mesorhizobium ciceri strain CC1192 plasmid pMc1192, complete sequence) position: , mismatch: 6, identity: 0.793

tccaaccggtccgacgggagcaaccggtc	CRISPR spacer
tgcgaccggttcgactggagcaaccggct	Protospacer
* *.******.**** ***********..

29. spacer 2.4|878190|29|NZ_CP020352|CRT matches to NZ_CP015063 (Mesorhizobium ciceri strain CC1192 plasmid pMc1192, complete sequence) position: , mismatch: 6, identity: 0.793

tccaaccggtccgacgggagcaaccggtc	CRISPR spacer
tgcgaccggttcgactggagcaaccggct	Protospacer
* *.******.**** ***********..

30. spacer 2.12|878712|29|NZ_CP020352|CRT matches to NZ_CP011054 (Halomonas sp. KO116 plasmid unnamed2, complete sequence) position: , mismatch: 6, identity: 0.793

agcgacaggcgcaaccggagcgacaggag	CRISPR spacer
tggcacaggcgcaaccgaagcaacaggtg	Protospacer
 *  *************.***.***** *

31. spacer 2.13|878766|29|NZ_CP020352|CRT matches to NZ_CP011054 (Halomonas sp. KO116 plasmid unnamed2, complete sequence) position: , mismatch: 6, identity: 0.793

agcgacaggcgcaaccggagcgacaggcg	CRISPR spacer
tggcacaggcgcaaccgaagcaacaggtg	Protospacer
 *  *************.***.*****.*

32. spacer 2.14|878820|29|NZ_CP020352|CRT matches to NZ_CP016082 (Streptomyces sp. SAT1 plasmid unnamed2, complete sequence) position: , mismatch: 6, identity: 0.793

agcgacaggcgcaacgggagcgacaggcg	CRISPR spacer
agcgacaggagcgacgggagcgacgcgat	Protospacer
********* **.***********. *

33. spacer 2.14|878820|29|NZ_CP020352|CRT matches to NZ_CP032340 (Azospirillum brasilense strain MTCC4038 plasmid p1, complete sequence) position: , mismatch: 6, identity: 0.793

agcgacaggcgcaacgggagcgacaggcg	CRISPR spacer
tccggggggcgcaacgggagagacaggcg	Protospacer
  **. .************* ********

34. spacer 2.14|878820|29|NZ_CP020352|CRT matches to NZ_CP012915 (Azospirillum brasilense strain Sp 7 plasmid ABSP7_p1, complete sequence) position: , mismatch: 6, identity: 0.793

agcgacaggcgcaacgggagcgacaggcg	CRISPR spacer
tccggggggcgcaacgggagagacaggcg	Protospacer
  **. .************* ********

35. spacer 2.23|879495|29|NZ_CP020352|CRT matches to CP024687 (Bacillus wiedmannii bv. thuringiensis strain FCC41 plasmid pFCC41-3-257K, complete sequence) position: , mismatch: 6, identity: 0.793

cccgacaggtccaactggcccgacaggtc	CRISPR spacer
agtaacaggtccaactggcccaacagggc	Protospacer
  ..*****************.***** *

36. spacer 2.23|879495|29|NZ_CP020352|CRT matches to NZ_MF600313 (Mycolicibacterium sp. CBMA 213 plasmid pCBMA213_1, complete sequence) position: , mismatch: 6, identity: 0.793

cccgacaggtccaactggcccgacaggtc	CRISPR spacer
ctgaccaggtcaaaccggcccgacaggtc	Protospacer
*. . ****** ***.*************

37. spacer 2.23|879495|29|NZ_CP020352|CRT matches to NZ_CP013556 (Rhizobium phaseoli strain N931 plasmid pRphaN931d, complete sequence) position: , mismatch: 6, identity: 0.793

cccgacaggtccaactggcccgacaggtc--	CRISPR spacer
accgacaggtcgaactggcccg--cgatcaa	Protospacer
 ********** **********   *.**

38. spacer 2.23|879495|29|NZ_CP020352|CRT matches to NZ_CP013567 (Rhizobium phaseoli strain N831 plasmid pRphaN831d, complete sequence) position: , mismatch: 6, identity: 0.793

cccgacaggtccaactggcccgacaggtc--	CRISPR spacer
accgacaggtcgaactggcccg--cgatcaa	Protospacer
 ********** **********   *.**

39. spacer 2.4|878190|29|NZ_CP020352|CRT matches to NZ_CP043441 (Cupriavidus campinensis strain MJ1 plasmid unnamed1, complete sequence) position: , mismatch: 7, identity: 0.759

tccaaccggtccgacgggagcaaccggtc	CRISPR spacer
gccaaccggtccgactggaccaacgcccc	Protospacer
 ************** *** ****   .*

40. spacer 2.11|878649|38|NZ_CP020352|CRT matches to CP024687 (Bacillus wiedmannii bv. thuringiensis strain FCC41 plasmid pFCC41-3-257K, complete sequence) position: , mismatch: 7, identity: 0.816

cccaacaggtccaacaggcgcaaccggagcgacaggcg	CRISPR spacer
cccaacaggaccaacaggggcaaccggactaacgggtg	Protospacer
********* ******** ********* ..**.**.*

41. spacer 2.12|878712|29|NZ_CP020352|CRT matches to NZ_CP033971 (Cupriavidus pauculus strain FDAARGOS_614 plasmid unnamed2, complete sequence) position: , mismatch: 7, identity: 0.759

agcgacaggcgcaaccggagcgacaggag	CRISPR spacer
ctccacaggcgcaaccggcgccacaggtc	Protospacer
  * ************** ** *****

42. spacer 2.13|878766|29|NZ_CP020352|CRT matches to NZ_CP033971 (Cupriavidus pauculus strain FDAARGOS_614 plasmid unnamed2, complete sequence) position: , mismatch: 7, identity: 0.759

agcgacaggcgcaaccggagcgacaggcg	CRISPR spacer
ctccacaggcgcaaccggcgccacaggtc	Protospacer
  * ************** ** *****.

43. spacer 2.14|878820|29|NZ_CP020352|CRT matches to NZ_CP009336 (Bacillus thuringiensis strain HD1011 plasmid 1, complete sequence) position: , mismatch: 7, identity: 0.759

agcgacaggcgcaacgggagcgacaggcg	CRISPR spacer
tgaaacaggagcaacgggagcgacgggaa	Protospacer
 * .***** **************.** .

44. spacer 2.14|878820|29|NZ_CP020352|CRT matches to NZ_CP009336 (Bacillus thuringiensis strain HD1011 plasmid 1, complete sequence) position: , mismatch: 7, identity: 0.759

agcgacaggcgcaacgggagcgacaggcg	CRISPR spacer
tgaaacaggagcaacgggaacgacaggaa	Protospacer
 * .***** *********.******* .

45. spacer 2.23|879495|29|NZ_CP020352|CRT matches to HQ632825 (Prochlorococcus phage P-SSM5 genomic sequence) position: , mismatch: 7, identity: 0.759

cccgacaggtccaactggcccgacaggtc	CRISPR spacer
tggatcaggtcctactggtccgacaggtc	Protospacer
.  . ******* *****.**********

46. spacer 2.23|879495|29|NZ_CP020352|CRT matches to HQ632825 (Prochlorococcus phage P-SSM5 genomic sequence) position: , mismatch: 7, identity: 0.759

cccgacaggtccaactggcccgacaggtc	CRISPR spacer
aaatccaggtccaactggtcccacaggtc	Protospacer
     *************.** *******

47. spacer 2.23|879495|29|NZ_CP020352|CRT matches to AY939844 (Prochlorococcus phage P-SSM2, complete genome) position: , mismatch: 7, identity: 0.759

cccgacaggtccaactggcccgacaggtc	CRISPR spacer
aaatccaggtccaactggtcccacaggtc	Protospacer
     *************.** *******

48. spacer 2.23|879495|29|NZ_CP020352|CRT matches to AY939844 (Prochlorococcus phage P-SSM2, complete genome) position: , mismatch: 7, identity: 0.759

cccgacaggtccaactggcccgacaggtc	CRISPR spacer
tggatcaggtcctactggtccgacaggtc	Protospacer
.  . ******* *****.**********

49. spacer 2.23|879495|29|NZ_CP020352|CRT matches to CP024687 (Bacillus wiedmannii bv. thuringiensis strain FCC41 plasmid pFCC41-3-257K, complete sequence) position: , mismatch: 7, identity: 0.759

cccgacaggtccaactggcccgacaggtc	CRISPR spacer
agtaacaggtccaactggcccaatagggc	Protospacer
  ..*****************.*.*** *

50. spacer 2.23|879495|29|NZ_CP020352|CRT matches to NZ_CP012101 (Bacillus thuringiensis strain HS18-1 plasmid pHS18-2, complete sequence) position: , mismatch: 7, identity: 0.759

cccgacaggtccaactggcccgacaggtc	CRISPR spacer
tataacaggtccaactggacctacagggc	Protospacer
. ..************** ** ***** *

51. spacer 2.23|879495|29|NZ_CP020352|CRT matches to NZ_CP012101 (Bacillus thuringiensis strain HS18-1 plasmid pHS18-2, complete sequence) position: , mismatch: 7, identity: 0.759

cccgacaggtccaactggcccgacaggtc	CRISPR spacer
tataacaggtccaactggacctacagggc	Protospacer
. ..************** ** ***** *

52. spacer 2.23|879495|29|NZ_CP020352|CRT matches to NZ_CP012101 (Bacillus thuringiensis strain HS18-1 plasmid pHS18-2, complete sequence) position: , mismatch: 7, identity: 0.759

cccgacaggtccaactggcccgacaggtc	CRISPR spacer
tgtaacaggtccgactggcccaacaggcc	Protospacer
. ..********.********.*****.*

53. spacer 2.23|879495|29|NZ_CP020352|CRT matches to NZ_CP012105 (Bacillus thuringiensis strain HS18-1 plasmid pHS18-6, complete sequence) position: , mismatch: 7, identity: 0.759

cccgacaggtccaactggcccgacaggtc	CRISPR spacer
aggaacaggtccaactggtcctacagggc	Protospacer
   .**************.** ***** *

54. spacer 2.23|879495|29|NZ_CP020352|CRT matches to MN693383 (Marine virus AFVG_25M295, complete genome) position: , mismatch: 7, identity: 0.759

cccgacaggtccaactggcccgacaggtc	CRISPR spacer
tggtacaggtccaactggtcctacaggac	Protospacer
.   **************.** ***** *

55. spacer 2.23|879495|29|NZ_CP020352|CRT matches to MN693263 (Marine virus AFVG_25M296, complete genome) position: , mismatch: 7, identity: 0.759

cccgacaggtccaactggcccgacaggtc	CRISPR spacer
tggagcaggtccaactggccctgcaggtc	Protospacer
.  ..**************** .******

56. spacer 2.24|879549|29|NZ_CP020352|CRT matches to CP024687 (Bacillus wiedmannii bv. thuringiensis strain FCC41 plasmid pFCC41-3-257K, complete sequence) position: , mismatch: 7, identity: 0.759

cccgacgggtccaactggcccgacaggtc	CRISPR spacer
agtaacaggtccaactggcccaacagggc	Protospacer
  ..**.**************.***** *

57. spacer 2.5|878244|38|NZ_CP020352|CRT matches to MN694114 (Marine virus AFVG_250M252, complete genome) position: , mismatch: 8, identity: 0.789

tccaacaggcgcaaccggagcaacaggcgcaacgggag	CRISPR spacer
cacgcagggcgcaacgggagcaaccggcgcaacgggag	Protospacer
. *.  .******** ******** *************

58. spacer 2.19|879207|47|NZ_CP020352|CRT matches to MN694114 (Marine virus AFVG_250M252, complete genome) position: , mismatch: 8, identity: 0.83

cccaaccggagcaacgggagcaaccggcccaacgggagcaaccggcc	CRISPR spacer
cacgcagggcgcaacgggagcaaccggcgcaacgggagcaaccggcg	Protospacer
* *.   ** ****************** *****************

59. spacer 2.20|879279|38|NZ_CP020352|CRT matches to KY421186 (Flavobacterium phage FL-1, complete genome) position: , mismatch: 9, identity: 0.763

agcaaccggggcaaccggagcaacgggagcaaccggcc	CRISPR spacer
ttaaaccggagcaacgggagcaacgggagcaacggaag	Protospacer
   ******.***** ***************** *.

60. spacer 2.5|878244|38|NZ_CP020352|CRT matches to NZ_CP011054 (Halomonas sp. KO116 plasmid unnamed2, complete sequence) position: , mismatch: 10, identity: 0.737

tccaacaggcgcaaccggagcaacaggcgcaacgggag	CRISPR spacer
tggcacaggcgcaaccgaagcaacaggtgcaaattgca	Protospacer
*   *************.*********.****   * .

61. spacer 2.20|879279|38|NZ_CP020352|CRT matches to CP011076 (Brevibacillus laterosporus strain B9 plasmid unnamed2, complete sequence) position: , mismatch: 11, identity: 0.711

agcaaccggggcaaccggagcaacgggagcaaccggcc	CRISPR spacer
ggcaacaggggcaaccggagcaaccggaggtattttag	Protospacer
.***** ***************** ****  *..

62. spacer 2.3|878118|47|NZ_CP020352|CRT matches to MN694114 (Marine virus AFVG_250M252, complete genome) position: , mismatch: 12, identity: 0.745

agcaaccggtccgacgggagcaaccggcccaacaggagcaaccggcc	CRISPR spacer
cacgcagggcgcaacgggagcaaccggcgcaacgggagcaaccggcg	Protospacer
 .*.   **. *.*************** ****.************

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

739689 : 749614

Synechococcus_phage(50.0%)

The bacterium proteins that are colored denote the protein is present at specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin' and 'tRNA')

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_020450435.1\|739689_740985_+	adenylosuccinate lyase	A0A2H4UUU6	Bodo_saltans_virus	5.9e-19	27.1
WP_020450436.1\|741059_741776_+	phosphoribosylaminoimidazolesuccinocarboxamide synthase	A0A0E3G7P8	Synechococcus_phage	7.4e-48	43.2
WP_003179532.1\|741777_742032_+	phosphoribosylformylglycinamidine synthase subunit PurS	M4QPE7	Synechococcus_phage	8.0e-05	33.3
WP_080131266.1\|742028_742712_+	phosphoribosylformylglycinamidine synthase subunit PurQ	NA	NA	NA	NA
WP_020450438.1\|742695_744924_+	phosphoribosylformylglycinamidine synthase subunit PurL	A6N228	Microbacterium_phage	3.8e-159	41.9
WP_023855588.1\|744899_746330_+	amidophosphoribosyltransferase	A0A0M3SGR2	Mollivirus	4.5e-52	32.9
WP_035337048.1\|746454_747495_+	phosphoribosylformylglycinamidine cyclo-ligase	A0A0E3F760	Synechococcus_phage	2.7e-62	46.3
WP_035337045.1\|747491_748079_+	phosphoribosylglycinamide formyltransferase	M4QRX9	Synechococcus_phage	7.0e-28	38.9
WP_035337043.1\|748075_749614_+	bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase	Q58MG4	Prochlorococcus_phage	5.6e-77	52.0

DBSCAN-SWA_2

1358956 : 1385662

Bacillus_phage(25.0%)

holin,coat

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_080131326.1\|1358956_1359436_+\|coat	spore coat protein CotO	NA	NA	NA	NA
WP_023856676.1\|1359472_1359922_-\|coat	spore coat protein	NA	NA	NA	NA
WP_003180694.1\|1360072_1360555_-\|coat	spore coat protein	NA	NA	NA	NA
WP_003180696.1\|1360687_1361194_-\|coat	spore coat protein	NA	NA	NA	NA
WP_003180698.1\|1361261_1361615_-	hypothetical protein	NA	NA	NA	NA
WP_003180700.1\|1361663_1362050_-\|coat	spore coat protein	NA	NA	NA	NA
WP_003180704.1\|1362197_1362554_+	DUF1360 domain-containing protein	NA	NA	NA	NA
WP_003180706.1\|1362840_1363050_+	hypothetical protein	NA	NA	NA	NA
WP_020450965.1\|1363129_1363261_+	YjcZ family sporulation protein	NA	NA	NA	NA
WP_003180710.1\|1363387_1363645_+	sporulation protein	NA	NA	NA	NA
WP_059261123.1\|1363683_1365963_-	ATP-dependent helicase	A0A068EQC7	Bacillus_phage	6.6e-90	35.5
WP_003180714.1\|1366084_1366342_+	hypothetical protein	NA	NA	NA	NA
WP_003180716.1\|1366381_1366969_-	DedA family protein	NA	NA	NA	NA
WP_003180718.1\|1367064_1368051_-	UDP-glucose 4-epimerase GalE	A0A2K9L5H6	Tupanvirus	3.6e-53	38.1
WP_080131327.1\|1368047_1368938_-	UTP--glucose-1-phosphate uridylyltransferase GalU	A0A127AW70	Bacillus_phage	1.5e-85	56.7
WP_020450971.1\|1368960_1369356_-	GtrA family protein	NA	NA	NA	NA
WP_003180726.1\|1369521_1369941_-	GNAT family N-acetyltransferase	NA	NA	NA	NA
WP_003180728.1\|1369950_1370460_-	hypothetical protein	NA	NA	NA	NA
WP_003180731.1\|1370524_1371247_-	esterase family protein	NA	NA	NA	NA
WP_003180732.1\|1371358_1371814_-	hypothetical protein	NA	NA	NA	NA
WP_003180734.1\|1371825_1372314_-	DNA damage-inducible protein DinB	NA	NA	NA	NA
WP_003180735.1\|1372394_1373342_-	YafY family transcriptional regulator	NA	NA	NA	NA
WP_003180736.1\|1373651_1374776_+	methionine biosynthesis PLP-dependent protein	A0A0B5JD48	Pandoravirus	9.0e-16	25.6
WP_035338954.1\|1374765_1375941_+	cystathionine beta-lyase	A0A0B5JD48	Pandoravirus	4.8e-20	28.0
WP_035338952.1\|1375985_1377176_-	DUF819 domain-containing protein	NA	NA	NA	NA
WP_035338950.1\|1377347_1377917_+	MarR family transcriptional regulator	NA	NA	NA	NA
WP_003180743.1\|1377906_1378191_+	hypothetical protein	NA	NA	NA	NA
WP_003180744.1\|1378368_1379742_+	serine hydrolase	A0A1D8EXR7	Mycobacterium_phage	3.4e-09	24.6
WP_035334158.1\|1379836_1380271_+	SMI1/KNR4 family protein	NA	NA	NA	NA
WP_051967072.1\|1380358_1381309_-	family 43 glycosylhydrolase	NA	NA	NA	NA
WP_020450980.1\|1382337_1382670_-	hypothetical protein	NA	NA	NA	NA
WP_020450981.1\|1382681_1384124_-	hypothetical protein	NA	NA	NA	NA
WP_080131328.1\|1384234_1384516_+	DUF1433 domain-containing protein	NA	NA	NA	NA
WP_044805482.1\|1384593_1384770_-	helix-turn-helix transcriptional regulator	NA	NA	NA	NA
WP_020450984.1\|1385113_1385383_+	phage-like protein XhlA	A0A2H4JD40	uncultured_Caudovirales_phage	3.7e-24	62.9
WP_020450985.1\|1385398_1385662_+\|holin	phage holin	A0A2H4J6M0	uncultured_Caudovirales_phage	1.5e-30	77.0

DBSCAN-SWA_3

1403050 : 1429188

Bacillus_phage(23.08%)

terminase,holin,plate,capsid,portal,tail

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_020451003.1\|1403050_1403698_+	poly-gamma-glutamate hydrolase family protein	A0A0Y0AJU6	Bacillus_phage	2.6e-44	46.5
WP_020451004.1\|1403710_1404367_-	ImmA/IrrE family metallo-endopeptidase	A0A2H4JA43	uncultured_Caudovirales_phage	6.8e-40	49.7
WP_020451005.1\|1404555_1404909_-	helix-turn-helix transcriptional regulator	A0A2H4J6F9	uncultured_Caudovirales_phage	3.6e-19	49.6
WP_023856709.1\|1405081_1405342_+	helix-turn-helix transcriptional regulator	S5MA07	Brevibacillus_phage	3.0e-07	43.8
WP_033155144.1\|1405331_1405628_+	hypothetical protein	NA	NA	NA	NA
WP_023856711.1\|1405628_1406453_+\|portal	phage portal protein	S6BFM4	Thermus_phage	2.1e-25	29.6
WP_025811606.1\|1406352_1407153_+	ATP-binding protein	Q0H276	Geobacillus_phage	8.0e-59	47.7
WP_023856714.1\|1407422_1407764_+	hypothetical protein	NA	NA	NA	NA
WP_020451011.1\|1407760_1407964_+	hypothetical protein	A0A2H4J4M6	uncultured_Caudovirales_phage	6.4e-13	56.2
WP_080131330.1\|1408081_1408585_+	sigma-70 family RNA polymerase sigma factor	A0A0K2CNQ1	Brevibacillus_phage	4.9e-22	39.3
WP_026579866.1\|1408729_1409530_+\|terminase	terminase	A0A0S2MVB6	Bacillus_phage	2.6e-65	51.4
WP_080131331.1\|1409526_1410825_+\|terminase	PBSX family phage terminase large subunit	M4ZRM5	Bacillus_phage	1.1e-150	61.5
WP_020451015.1\|1410828_1412343_+\|portal	phage portal protein	A0A1B1P7C8	Bacillus_phage	6.4e-142	55.3
WP_020451016.1\|1412350_1413199_+	hypothetical protein	A0A1B1P7E4	Bacillus_phage	8.8e-56	60.6
WP_020451017.1\|1413216_1414152_+\|capsid	phage capsid protein	A0A1B1P7E3	Bacillus_phage	3.6e-103	64.1
WP_020451018.1\|1414261_1414642_+	DUF3199 family protein	NA	NA	NA	NA
WP_026579862.1\|1414638_1414995_+	DUF3599 family protein	NA	NA	NA	NA
WP_080131332.1\|1414991_1415480_+	HK97 gp10 family phage protein	A0A249XXA4	Clostridium_phage	1.1e-34	43.3
WP_035338940.1\|1415492_1415933_+	hypothetical protein	NA	NA	NA	NA
WP_020451022.1\|1415933_1416158_+	hypothetical protein	NA	NA	NA	NA
WP_080131333.1\|1416157_1417504_+\|tail	phage tail sheath protein	S5MNC1	Brevibacillus_phage	5.3e-79	40.2
WP_003180830.1\|1417505_1417949_+\|tail	phage tail tube protein	A0A0K2CNG3	Brevibacillus_phage	2.1e-24	44.7
WP_020451025.1\|1418106_1418556_+	hypothetical protein	A0A0A7RTY8	Clostridium_phage	4.1e-12	38.7
WP_095290311.1\|1418597_1418735_+	hypothetical protein	NA	NA	NA	NA
WP_020451027.1\|1418738_1422503_+	transglycosylase SLT domain-containing protein	A0A1L2JY60	Aeribacillus_phage	3.4e-43	48.1
WP_080131334.1\|1422495_1423152_+	LysM peptidoglycan-binding domain-containing protein	A0A090DBR9	Clostridium_phage	6.9e-24	32.1
WP_080131335.1\|1423208_1424189_+\|portal	phage portal protein	H7BV96	unidentified_phage	1.3e-39	32.0
WP_023856723.1\|1424185_1424494_+	DUF2577 domain-containing protein	S6C459	Thermus_phage	1.4e-06	37.3
WP_020451031.1\|1424512_1424938_+	DUF2634 domain-containing protein	A0A2H4J6K5	uncultured_Caudovirales_phage	1.6e-13	37.3
WP_050820764.1\|1424930_1425974_+\|plate	baseplate J/gp47 family protein	S6AVU3	Thermus_phage	2.4e-71	43.5
WP_048350281.1\|1425960_1426881_+	YmfQ family protein	A0A0A7RTT8	Clostridium_phage	1.8e-14	31.7
WP_020451034.1\|1426894_1427281_+	phage protein	NA	NA	NA	NA
WP_020451036.1\|1428637_1428907_+	PBSX phage-like protein XhlA	A0A2H4JD40	uncultured_Caudovirales_phage	3.3e-25	64.0
WP_041817189.1\|1428921_1429188_+\|holin	phage holin	A0A2H4J6M0	uncultured_Caudovirales_phage	1.2e-27	63.2

DBSCAN-SWA_4

2494477 : 2506117

Staphylococcus_phage(55.56%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_023857488.1\|2494477_2495071_-	SMC-Scp complex subunit ScpB	A0A1B1IVT7	uncultured_Mediterranean_phage	2.6e-14	37.6
WP_035338491.1\|2495060_2495816_-	segregation/condensation protein A	A0A1B1IVW1	uncultured_Mediterranean_phage	4.7e-08	37.5
WP_003183108.1\|2495998_2496094_+	YjcZ family sporulation protein	NA	NA	NA	NA
WP_048350675.1\|2496213_2496735_+	DUF309 domain-containing protein	NA	NA	NA	NA
WP_020452011.1\|2496745_2497120_-	GNAT family N-acetyltransferase	NA	NA	NA	NA
WP_003183115.1\|2497221_2497686_-	6,7-dimethyl-8-ribityllumazine synthase	A0A2H4PQS3	Staphylococcus_phage	1.7e-45	70.7
WP_048350676.1\|2497720_2498917_-	bifunctional 3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP cyclohydrolase II	A0A2H4PQS2	Staphylococcus_phage	9.6e-117	54.2
WP_048350677.1\|2498938_2499586_-	riboflavin synthase	A0A2H4PQS5	Staphylococcus_phage	2.0e-39	44.2
WP_048350678.1\|2499597_2500686_-	bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD	A0A2H4PQS8	Staphylococcus_phage	4.7e-62	37.5
WP_020452015.1\|2501048_2501393_-	hypothetical protein	NA	NA	NA	NA
WP_080131432.1\|2501655_2503845_-	AAA family ATPase	A0A220BYT7	Staphylococcus_phage	1.4e-153	41.5
WP_023857494.1\|2504061_2504331_-	hypothetical protein	NA	NA	NA	NA
WP_043054368.1\|2504320_2505451_-	tetratricopeptide repeat protein	A0A1P8CWN8	Bacillus_phage	1.8e-72	41.2
WP_020452019.1\|2505679_2506117_-	peptidylprolyl isomerase	A0A2H4UTF4	Bodo_saltans_virus	9.2e-17	44.6

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

Overview of predicted results

Overview of the results

Cas Category Instructions

Results visualization

1. NZ_CP020352

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CRISPR-Cas detection and classification

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Click the colored protein region to show detailed information

Click the colored protein region to show detailed information

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Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection