CRISPRimmunity

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Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Self targeting spacer number	Target MGE spacer number	Prophage number	Anti-CRISPR protein number
NZ_CP020449	Streptococcus agalactiae strain FDAARGOS_254 chromosome, complete genome	1 crisprs	RT,cas3,csa3,DEDDh,csm6,csn2,cas2,cas1,cas9,DinG	0	5	9	0

Results visualization

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CRISPR-Cas detection and classification

Crispr_ID: NZ_CP020449_1

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

NZ_CP020449_1

1582185-1582409

TypeII

II-A

Consensus_repeat	Method
CATTCGAAACAGCACAGCTCTAAAACC	CRT
AGTTTTGGAACCATTCGAAACAGCACAGCTCTAAAACC	PILER-CR
GTTTTGGAACCATTCGAAACAGCACAGCTCTAAAAC	CRISPRCasFinder

3 spacers

csn2,cas2,cas1,cas9

The CRISPR arrays of NZ_CP020449_1

>merge|NZ_CP020449|1|1582185-1582409|CRT,PILER-CR,CRISPRCasFinder
GCATAATAACCGCGCAGCTCTAAAACCGTCCAGGAATCTATATAGACGATATTAAAGTTTTGGAACCATTCGAAACAGCACAGCTCTAAAACACTGTTCGCTGTTTGTGCGACTATTGTATTGTTTTGGAATCATTCGAAACAGCACAGCTCTAAAACCATGTTGTTCCATCATCAAATAACATTGCAGTTTTGGAACCATTCGAAACAGCACAGCTCTAAAACC

>NZ_CP020449|1|1|1582185-1582409|CRT
GCATAATAACCGCGCAGCTCTAAAACC	GTCCAGGAATCTATATAGACGATATTAAAGTTTTGGAAC
CATTCGAAACAGCACAGCTCTAAAACA	CTGTTCGCTGTTTGTGCGACTATTGTATTGTTTTGGAAT
CATTCGAAACAGCACAGCTCTAAAACC	ATGTTGTTCCATCATCAAATAACATTGCAGTTTTGGAAC
CATTCGAAACAGCACAGCTCTAAAACC

>NZ_CP020449|1|1|1582240-1582409|PILER-CR
AGTTTTGGAACCATTCGAAACAGCACAGCTCTAAAACA	CTGTTCGCTGTTTGTGCGACTATTGTAT
TGTTTTGGAATCATTCGAAACAGCACAGCTCTAAAACC	ATGTTGTTCCATCATCAAATAACATTGC
AGTTTTGGAACCATTCGAAACAGCACAGCTCTAAAACC

>NZ_CP020449|1|1|1582241-1582408|CRISPRCasFinder
GTTTTGGAACCATTCGAAACAGCACAGCTCTAAAAC	ACTGTTCGCTGTTTGTGCGACTATTGTATT
GTTTTGGAATCATTCGAAACAGCACAGCTCTAAAAC	CATGTTGTTCCATCATCAAATAACATTGCA
GTTTTGGAACCATTCGAAACAGCACAGCTCTAAAAC

Protein	Signature genes	Signature genes Name	Protein_function
NZ_CP020449.2\|WP_000678289.1\|1576370_1579004_-\|pneumococcal-type-histidine-triad-protein	unknown	unknown	gnl\|CDD\|380193
NZ_CP020449.2\|WP_000122194.1\|1583160_1583502_-\|CRISPR-associated-endonuclease-Cas2	cas2	COG3512_cas2_CAS-II-A:CAS-II-B:CAS-II-C	gnl\|CDD\|226043
NZ_CP020449.2\|WP_000159234.1\|1592643_1593015_-\|YbgA-family-protein	unknown	unknown	gnl\|CDD\|377982
NZ_CP020449.2\|WP_000168908.1\|1571759_1574552_-\|cation-transporting-P-type-ATPase	unknown	unknown	gnl\|CDD\|223550
NZ_CP020449.2\|WP_000146568.1\|1569899_1570538_+\|CatB-related-O-acetyltransferase	unknown	unknown	gnl\|CDD\|100040
NZ_CP020449.2\|WP_000665410.1\|1574704_1575139_-\|peptide-methionine-(R)-S-oxide-reductase-MsrB	unknown	unknown	gnl\|CDD\|234692
NZ_CP020449.2\|WP_001241816.1\|1592238_1592619_-\|hypothetical-protein	unknown	unknown	gnl\|CDD\|131381
NZ_CP020449.2\|WP_000590703.1\|1582508_1583174_-\|type-II-A-CRISPR-associated-protein-Csn2	csn2	pfam09711_csn2_CAS-II-A	gnl\|CDD\|213408
NZ_CP020449.2\|WP_000914659.1\|1591015_1592146_-\|oxygen-independent-coproporphyrinogen-III-oxidase	unknown	unknown	gnl\|CDD\|236309
NZ_CP020449.2\|WP_001019128.1\|1579249_1581082_-\|elongation-factor-4	unknown	unknown	gnl\|CDD\|235462
NZ_CP020449.2\|WP_000621143.1\|1575138_1575603_-\|GNAT-family-N-acetyltransferase	unknown	unknown	gnl\|CDD\|366181
NZ_CP020449.2\|WP_001040096.1\|1584369_1588482_-\|type-II-CRISPR-RNA-guided-endonuclease-Cas9	cas9	COG3513_cas9_CAS-II-A:CAS-II-B:CAS-II-C	gnl\|CDD\|226044
NZ_CP020449.2\|WP_000323544.1\|1589503_1590274_-\|TIGR01457-family-HAD-type-hydrolase	unknown	unknown	gnl\|CDD\|319832
NZ_CP020449.2\|WP_000523795.1\|1590274_1591012_-\|acyl-ACP-thioesterase	unknown	unknown	gnl\|CDD\|366738
NZ_CP020449.2\|WP_000929489.1\|1583498_1584368_-\|type-II-CRISPR-associated-endonuclease-Cas1	cas1	cd09720_cas1_CAS-II-A:CAS-II-B:CAS-II-C	gnl\|CDD\|274694
NZ_CP020449.2\|WP_000653355.1\|1588857_1589514_-\|TIGR01906-family-membrane-protein	unknown	unknown	gnl\|CDD\|226884
NZ_CP020449.2\|WP_000065324.1\|1570656_1571760_-\|DUF2974-domain-containing-protein	unknown	unknown	gnl\|CDD\|371412
NZ_CP020449.2\|WP_000438313.1\|1581217_1581634_-\|nucleoside-diphosphate-kinase	unknown	unknown	gnl\|CDD\|179085
NZ_CP020449.2\|WP_001056394.1\|1567040_1567652_-\|TVP38/TMEM64-family-protein	unknown	unknown	gnl\|CDD\|223475
NZ_CP020449.2\|WP_000180011.1\|1575611_1576271_-\|HD-domain-containing-protein	unknown	unknown	gnl\|CDD\|224336

Protein	Function_ID	Function_description	E-value
NZ_CP020449.2\|WP_000678289.1\|1576370_1579004_-\|pneumococcal-type-histidine-triad-protein	gnl\|CDD\|380193	NF033189, internalin_A, class 1 internalin InlA. Internalins, as found in the intracellular human pathogen Listeria monocytogenes, are paralogous surface or secreted proteins with an N-terminal signal peptide, leucine-rich repeats, and usually a C-terminal LPXTG processing and cell surface anchoring site. See PMID:17764999 for a general discussion of internalins. Members of this family are internalin A (InlA), a class 1 (LPXTG-type) internalin.	5.90067e-22
NZ_CP020449.2\|WP_000122194.1\|1583160_1583502_-\|CRISPR-associated-endonuclease-Cas2	gnl\|CDD\|226043	COG3512, COG3512, CRISPR-associated protein, Cas2 homolog [Defense mechanisms].	2.38084e-52
NZ_CP020449.2\|WP_000159234.1\|1592643_1593015_-\|YbgA-family-protein	gnl\|CDD\|377982	pfam08349, DUF1722, Protein of unknown function (DUF1722). This domain of unknown function is found in bacteria and archaea and is homologous to the hypothetical protein ybgA from E. coli.	6.08694e-28
NZ_CP020449.2\|WP_000168908.1\|1571759_1574552_-\|cation-transporting-P-type-ATPase	gnl\|CDD\|223550	COG0474, MgtA, Cation transport ATPase [Inorganic ion transport and metabolism].	0
NZ_CP020449.2\|WP_000146568.1\|1569899_1570538_+\|CatB-related-O-acetyltransferase	gnl\|CDD\|100040	cd03349, LbH_XAT, Xenobiotic acyltransferase (XAT): The XAT class of hexapeptide acyltransferases is composed of a large number of microbial enzymes that catalyze the CoA-dependent acetylation of a variety of hydroxyl-bearing acceptors such as chloramphenicol and streptogramin, among others. Members of this class of enzymes include Enterococcus faecium streptogramin A acetyltransferase and Pseudomonas aeruginosa chloramphenicol acetyltransferase. They contain repeated copies of a six-residue hexapeptide repeat sequence motif (X-[STAV]-X-[LIV]-[GAED]-X) and adopt a left-handed parallel beta helix (LbH) structure. The active enzyme is a trimer with CoA and substrate binding sites at the interface of two separate LbH subunits. XATs are implicated in inactivating xenobiotics leading to xenobiotic resistance in patients.	2.15538e-71
NZ_CP020449.2\|WP_000665410.1\|1574704_1575139_-\|peptide-methionine-(R)-S-oxide-reductase-MsrB	gnl\|CDD\|234692	PRK00222, PRK00222, peptide-methionine (R)-S-oxide reductase MsrB.	6.40832e-86
NZ_CP020449.2\|WP_001241816.1\|1592238_1592619_-\|hypothetical-protein	gnl\|CDD\|131381	TIGR02328, TIGR02328, conserved hypothetical protein. Members of this protein are found in a small number of taxonomically well separated species, yet are strongly conserved, suggesting lateral gene transfer. Members are found in Treponema denticola, Clostridium acetobutylicum, and several of the Firmicutes. The function of this protein is unknown. [Hypothetical proteins, Conserved].	6.45558e-80
NZ_CP020449.2\|WP_000590703.1\|1582508_1583174_-\|type-II-A-CRISPR-associated-protein-Csn2	gnl\|CDD\|213408	cd09758, Csn2, CRISPR/Cas system-associated protein Csn2. Csn2 is a Nmeni subtype-specific Cas protein, which may function in the adaptation process which mediates the incorporation of foreign nucleic acids into the microbial host genome. Csn 2 may interact directly with double-stranded DNA. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and associated Cas proteins comprise a system for heritable host defense by prokaryotic cells against phage and other foreign DNA. Csn2 has been predicted to be a functional analog of Cas4 based on anti-correlated phyletic patterns; also known as SPy1049 family.	4.68487e-122
NZ_CP020449.2\|WP_000914659.1\|1591015_1592146_-\|oxygen-independent-coproporphyrinogen-III-oxidase	gnl\|CDD\|236309	PRK08599, PRK08599, oxygen-independent coproporphyrinogen III oxidase.	0
NZ_CP020449.2\|WP_001019128.1\|1579249_1581082_-\|elongation-factor-4	gnl\|CDD\|235462	PRK05433, PRK05433, GTP-binding protein LepA; Provisional.	0
NZ_CP020449.2\|WP_000621143.1\|1575138_1575603_-\|GNAT-family-N-acetyltransferase	gnl\|CDD\|366181	pfam00583, Acetyltransf_1, Acetyltransferase (GNAT) family. This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.	1.29827e-18
NZ_CP020449.2\|WP_001040096.1\|1584369_1588482_-\|type-II-CRISPR-RNA-guided-endonuclease-Cas9	gnl\|CDD\|226044	COG3513, COG3513, Predicted CRISPR-associated nuclease, contains McrA/HNH-nuclease and RuvC-like nuclease domain [Defense mechanisms].	0
NZ_CP020449.2\|WP_000323544.1\|1589503_1590274_-\|TIGR01457-family-HAD-type-hydrolase	gnl\|CDD\|319832	cd07530, HAD_Pase_UmpH-like, UmpH/NagD family phosphatase, similar to Escherichia coli UmpH UMP phosphatase/NagD nucleotide phosphatase and Mycobacterium tuberculosis Rv1692 glycerol 3-phosphate phosphatase. Escherichia coli UmpH/NagD is a ribonucleoside tri-, di-, and monophosphatase with a preference for purines, it shows peak activity with UMP and functions in UMP-degradation. It is also an effective phosphatase with AMP, GMP and CMP. Mycobacterium tuberculosis phosphatase, Rv1692 is a glycerol 3-phosphate phosphatase. Rv1692 is the final enzyme involved in glycerophospholipid recycling/catabolism. This subfamily belongs to the UmpH/NagD phosphatase family, and to the haloacid dehalogenase-like (HAD) hydrolases, a large superfamily of diverse enzymes that catalyze carbon or phosphoryl group transfer reactions on a range of substrates, using an active site aspartate in nucleophilic catalysis. Members of this superfamily include 2-L-haloalkanoic acid dehalogenase, azetidine hydrolase, phosphonoacetaldehyde hydrolase, phosphoserine phosphatase, phosphomannomutase, P-type ATPases and many others. HAD hydrolases are found in all three kingdoms of life, and most genomes are predicted to contain multiple HAD-like proteins. Members possess a highly conserved alpha/beta core domain, and many also possess a small cap domain, the fold and function of which is variable. HAD hydrolases are sometimes referred to as belonging to the DDDD superfamily of phosphohydrolases.	1.19143e-132
NZ_CP020449.2\|WP_000523795.1\|1590274_1591012_-\|acyl-ACP-thioesterase	gnl\|CDD\|366738	pfam01643, Acyl-ACP_TE, Acyl-ACP thioesterase. This family consists of various acyl-acyl carrier protein (ACP) thioesterases (TE) these terminate fatty acyl group extension via hydrolysing an acyl group on a fatty acid.	3.68196e-111
NZ_CP020449.2\|WP_000929489.1\|1583498_1584368_-\|type-II-CRISPR-associated-endonuclease-Cas1	gnl\|CDD\|274694	TIGR03639, cas1_NMENI, CRISPR-associated endonuclease Cas1, subtype II/NMENI. The CRISPR-associated protein Cas1 is virtually universal to CRISPR systems. CRISPR, an acronym for Clustered Regularly Interspaced Short Palindromic Repeats, is a prokaryotic immunity system for foreign DNA, mostly from phage. CRISPR systems belong to different subtypes, distinguished by both nature of the repeats, the makeup of the cohort of associated Cas proteins, and by molecular phylogeny within the more universal Cas proteins such as this one. This model is of type EXCEPTION and provides more specific information than the EQUIVALOG model TIGR00287. It describes the Cas1 variant of the NMENI subtype of CRISPR/Cas system.	2.54315e-139
NZ_CP020449.2\|WP_000653355.1\|1588857_1589514_-\|TIGR01906-family-membrane-protein	gnl\|CDD\|226884	COG4478, COG4478, Predicted membrane protein [Function unknown].	8.37758e-51
NZ_CP020449.2\|WP_000065324.1\|1570656_1571760_-\|DUF2974-domain-containing-protein	gnl\|CDD\|371412	pfam11187, DUF2974, Protein of unknown function (DUF2974). This bacterial family of proteins has no known function.	2.245e-105
NZ_CP020449.2\|WP_000438313.1\|1581217_1581634_-\|nucleoside-diphosphate-kinase	gnl\|CDD\|179085	PRK00668, ndk, mulitfunctional nucleoside diphosphate kinase/apyrimidinic endonuclease/3'-; Validated.	6.25805e-79
NZ_CP020449.2\|WP_001056394.1\|1567040_1567652_-\|TVP38/TMEM64-family-protein	gnl\|CDD\|223475	COG0398, COG0398, Uncharacterized conserved protein [Function unknown].	6.1265e-31
NZ_CP020449.2\|WP_000180011.1\|1575611_1576271_-\|HD-domain-containing-protein	gnl\|CDD\|224336	COG1418, COG1418, Predicted HD superfamily hydrolase [General function prediction only].	2.46285e-32

>NZ_CP020449.2|WP_000438313.1|1581217_1581634_-|nucleoside-diphosphate-kinase
MEQTFFMIKPDGVKRGFIGEVISRIERRGFSIDRLEVRHADADILKRHYAELTDRPFFPTLVDYMTSGPVIIGVISGEEVISTWRTMMGSTNPKDALPGTIRGDFAQAPSPNQATCNIVHGSDSPESATREIAIWFNN
>NZ_CP020449.2|WP_001019128.1|1579249_1581082_-|elongation-factor-4
MNIEDLKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSREMQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLAACEGAILVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRAEVEDVIGLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGEVDAPLQALIFDSVYDAYRGVILQVRIINGMVKPGDKIQMMSNGKTFDVTEVGIFNPKAVGRDFLATGDVGYIAASIKTVADTRVGDTITLANNPAIEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQFEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVVYHVNTTDGEMLEVSNPSEFPDPTRVDSIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVNVIYQIPLAEIVFDFFDKLKSSTRGYASFDYEISEYRRSQLVKMDILLNGDKVDALSFIVHKEFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGGDVSRKRKLLEKQKAGKKRMKAIGSVEVPQEAFLSVLSMDDDDKK
>NZ_CP020449.2|WP_000678289.1|1576370_1579004_-|pneumococcal-type-histidine-triad-protein
MKGQKIIALAGLVLSCHFALTACHTQEHKNSHHIKTKQVAKKKANKKKVSVKESHKKRKGVAGVDFPTDDGFLLTKDSKILSHTDSGIVVAHGNHSHFIFYSDLKGSKFSYLIPNANAKTNKNQAVRNFKAGAVAVNTLNDGYVFNPADIVSEDANGYVVRHGDHFHYIPKASLSQQKQVQASRAVSRLGNQSNSHYRVNSSKIAGLHHPTSDGFLFNGQGIKGTTPTGILVEHHSHLHFISFADLRKGGWGSIADRYQPQKKVDSKKQSPSSKKPRNENTLPKDIKDKLAYLARELHLDISRIRVLKTLNGEIGFEYPHDDHTHVIMAKDIDLSKPIPNPHHDDEDHHKGHHHDESDHKHEEHEHTKSNKLSDEDQKKLIYLAEKLGLNPNQIEVLTSEDGSIIFKYPHDDHSHTIASKDIEIGKPIPDGHHDHSHAKDKVGMATLKQIGFDDEIIQDILHADAPTPFPSNETNPEKMRQWLATVTKINIGQRTNPFQRFGLSLMPNIEVLGIGFTPINDMTPVLQFKKLKQLWMTNTGITDYSFLDKMPLLEGLDISQNGIKDLSFLTKYKQLSLIAAANNGITSLKPLAELPNLQFLVLSHNNISDLTPLSNLTKLQELHLDHNNVKNLSALSGKKDLKVLDLSNNNSADLSTLKTTSLETLLLNETNTSNLSFLKQNPKVSNLTINNAKLSSLDGIEESDEIVKVEAEGNQIKSLVLKNKQGSLKFLNVTNNQLTSLEGVNNYTSLETLSVSKNKLESLDIKTPNKTVTNLDFSHNNVPTSQLKLNEKNIPEAVAKNFPAVVEGSMVGNGSLAEKAAMASKEEKQVSDNTNHQKNTEKSAQANADSKKENPKTHDEHHDHEETDHAHVGHHHH
>NZ_CP020449.2|WP_000180011.1|1575611_1576271_-|HD-domain-containing-protein
MTMVKREDVILNATENMVYHKLKNDPSGHDWFHIVRVRNLAVELAHKEGANTFICQMVALLHDIIDDKICQDSKQASYELTQWLYSQDLTIAEVEHILDILENISFKAGTGLTMKTLEGQIVQDADRLDAMGAIGIARTMAYSGSKGRLIHDPNLKPRENLTLEEYRNGQDTAIMHFYEKLLKLKDLMNTKQGKMLAQKRHDFLELYLAEFYAEWNGKR
>NZ_CP020449.2|WP_000621143.1|1575138_1575603_-|GNAT-family-N-acetyltransferase
MIRRAKEKDLPDIAELLKQILMLHHEVRPDIFHTRGSKFSKEQLKEMLMDESRPIFVYESDEGKVVAHLFLQLQEKRDLPRKSFKTLYIDDLCIDEEVRGQQIGQKLIDFARQYAKKHGCYNITLNVWNDNQRAVSFYEKLGFKPQQTQMEEIL
>NZ_CP020449.2|WP_000665410.1|1574704_1575139_-|peptide-methionine-(R)-S-oxide-reductase-MsrB
MKETQEELRQRIGHTAYQVTQNSATEHAFTGKYDDFFEEGIYVDIVSGEVLFSSLDKFQSGCGWPAFSKPIENRMVTNHQDHSHGMHRIEVRSRQADSHLGHVFNDGPVDAGGLRYCINSAALDFIPYDQMAKRGYGDYLSLFD
>NZ_CP020449.2|WP_000168908.1|1571759_1574552_-|cation-transporting-P-type-ATPase
MASTNNVSDTNTRFAKEELEEVFEELGTTQGGLSDEEVAVRQKKYGLNLLSEVKQESIILLFLKNFTSLMAILLWVGGFVAIVSNSLELGLAIWMVNVINGIFSFIQEYRASQATQALKKMLPSYSRVLRKSSEEKILSEQLVPGDIVLIEEGDRISADGRLIKTTDLQVNQSALTGESNPIYKDSNVENDQSKTLIECDNMVFAGTTVSSGSATMVVTAIGMQTQFGQIADLTQGMKSEKSPLQRELDRLTKQISIISITVGIIFFLAATFFVKEPVSKSFIFALGMIVAFIPEGLLPTVTLSLAMAVQRMAKEHALVKKLSSVETLGATSVICSDKTGTLTQNEMTVNRLWQNGKSYQVTGLGYAPEGQILFEGDNICFGNSDRGDLEKLIRFAHLCSNAQVLPPNDDRSTYTVLGDPTEACLNVLLEKSGINRQENRKFAPRLKELPFDSVRKRMTTIHSLGGDEKDKKISITKGAPKEILNLSDYVLSDDKVIPLNKEERNKIQLANDTFAKDGLRVLAVSYCDIEGFSKEQWTQENLEQHMVFIGLIAMSDPPREGVREAIDKCHAASIRIIMVTGDYGLTALSIAKNIGIIRNDNAKVISGLELSEMTDSQLKKELSGEVVFARVAPEQKYRVVTILQEMGEVVAVTGDGVNDAPALKKSDIGVAMGVTGTDVAKESADMILTDDHFASIVHAVEEGRAVYQNIKKFLTYIFNSNTPEAVPSAFFLFSKGFIPLPLTVMQILAVDLGTDMLPALGLGVEPPETDVMNRPPRRLTDRLLDKGLLIKSFLWYGTIESVLAMGGFFWAHYLRYGNFTFFVANGIPYREATTMTLGAIIFSQIGMVMNSRTSYQSIRALSIFGNKLINFGIIMEILAFLVLVYVPPFHNLFNTASLGLSHWLYLISCPFIMIGLDEVRKLFSSRKNKR
>NZ_CP020449.2|WP_000065324.1|1570656_1571760_-|DUF2974-domain-containing-protein
MSNIITYLKNNSNLTFDELALNDVDILCLNEFGYISFEKLINTTEMKSVLVCELYHEYLQTMAKSYSFMFTSQRHDLCQLMMTSKRFKNLTLSYYRAEVSLEFEKQFAAMVFTIPNIDYHQVVFRGTDANLIGWKEDFKLTYMREISAHRSAIKYLNTILPYFDKVVLSGHSKGGNLALYAAMFTKPDLKAKIDLIWLIDSPGLQKTLLPTTEYKTTKQKCIRLLPEESIVGMMLYSDIEPLIISSNARGILQHDVTTWEIQEPAILKTGAGLSLKSICFEKTFQQWMAELKSQERKLFFDLLFDSFLSSGVSSLDDFNLASRAKMMKAFHSFRELDDDKKRLFNKSLKLLVTIFWGAYHDNSRKTK
>NZ_CP020449.2|WP_000146568.1|1569899_1570538_+|CatB-related-O-acetyltransferase
MASILKKHLEKRLELKNDPMRTDMSTGASYPKYGFSVGKYTYGYQQFFYEGVNLKEIGAFCSIAQNVTITGMNHPTDHITTNPFIYYKSRGFINEDRADLIDEKKNGKVIIGNDVWIGTNVTILPSVTIGNGAIIGAGSVITKDIPDYAVVAGKPAKIIKYRFSEEEITLLNASQWWNWSDEAIKEHISEFSDKKEFFNTLKSISENKNHKL
>NZ_CP020449.2|WP_001056394.1|1567040_1567652_-|TVP38/TMEM64-family-protein
MNMKLSKRYRFWQKVIKALGVLALIATLVLVVYLYKLGILNDSNELKDLVHKYEFWGPMIFIVAQIVQIVFPVIPGGVTTVAGFLIFGPTLGFIYNYIGIIIGSVILFWLVKFYGRKFVLLFMDQKTFDKYESKLETSGYEKFFIFCMASPISPADIMVMITGLSNMSIKRFVTIIMITKPISIIGYSYLWIYGGDILKNFLN
>NZ_CP020449.2|WP_000590703.1|1582508_1583174_-|type-II-A-CRISPR-associated-protein-Csn2
MIKINFPILDEPLVLSNATILTIEDVSVYSSLVKHFYQYDVDEHLKLFDDKQKSLKAAELMLVTDILGYDVNSAPILKLIHGDLENQFNEKPEVKSMVEKLAATITELIAFECLENELDLEYDEITILELIKALGVKIETQSDTIFEKCFEIIQVYHYLTKKNLLVFVNSGAYLTKDEVIKLCEYINLMQKSVLFLEPRRLYDLPQYVIDKDYFLIGENMV
>NZ_CP020449.2|WP_000122194.1|1583160_1583502_-|CRISPR-associated-endonuclease-Cas2
MSYRYMRMILMFDMPTETAEERKAYRKFRKFLLSEGFIMHQFSVYSKLLLNNTANNAMIGRLKVNNPKKGNITLLTVTEKQFARMVYLHGERNTSVANSDSRLVFLGDSYDQD
>NZ_CP020449.2|WP_000929489.1|1583498_1584368_-|type-II-CRISPR-associated-endonuclease-Cas1
MAGWRTVVVNTHSKLSYKNNHLIFKDSYQTEMIHLSEIDILIMETTDIVLSTMLIKRLVDENILVIFCDDKRLPTAMLMPYYARHDSSLQLSRQMSWIEDVKADVWTSIIAQKILNQSFYLGECSFFEKSQSIMNLYHDLEPFDPSNREGHAARIYFNTLFGNDFSREQDNPINAGLDYGYSLLLSMFAREVVKCGCMTQFGLKHANQFNQFNLASDIMEPFRPIVDRIIYENRQSDFVKMKRELFSMFSETYSYNGKEMYLSNIVSDYTKKVIKSLNSDGNGIPEFRI
>NZ_CP020449.2|WP_001040096.1|1584369_1588482_-|type-II-CRISPR-RNA-guided-endonuclease-Cas9
MNKPYSIGLDIGTNSVGWSIITDDYKVPAKKMRVLGNTDKEYIKKNLIGALLFDGGNTAADRRLKRTARRRYTRRRNRILYLQEIFAEEMSKVDDSFFHRLEDSFLVEEDKRGSKYPIFATMQEEKYYHEKFPTIYHLRKELADKKEKADLRLIYLALAHIIKFRGHFLIEDDRFDVRNTDIQKQYQAFLEIFDTTFENNDLLSQNVDVEAILTDKISKSAKKDRILARYPNQKSTGIFAEFLKLIVGNQADFKKHFNLEDKTPLQFAKDSYDEDLENLLGQIGDEFADLFSAAKKLYDSVLLSGILTVTDLSTKAPLSASMIQRYDEHREDLKQLKQFVKASLPEKYQEIFADSSKDGYAGYIEGKTNQEAFYKYLSKLLTKQEGSEYLLEKIKNEDFLRKQRTFDNGSIPHQVHLTELRAIIRRQSEYYPFLKENLDRIEKILTFRIPYYVGPLAREKSDFAWMTRKTDDSIRPWNFEDLVDKEKSAEAFIHRMTNNDLYLPEEKVLPKHSLIYEKFTVYNELTKVRFLAEGFKDFQFLNRKQKETIFNSLFKEKRKVTEKDIISFLNKVDGYEGIAIKGIEKQFNASLSTYHDLKKILGKDFLDNTDNELILEDIVQTLTLFEDREMIRKRLDIYKDFFTESQLKKLYRRHYTGWGRLSAKLINGIRNKENQKTILDYLIDDGSANRNFMQLIKDAGLSFKPIIDKARTGSHLDNLKEVVGELAGSPAIKKGILQSLKIVDELVKVMGYEPEQIVVEMARENQTTAKGLSRSRQRLTTLRESLANLKSNILEEKKPKYVKDQVENHHLSDDRLFLYYLQNGKDMYTDDELDIDNLSQYDIDHIIPQAFIKDDSIDNRVLVSSAKNRGKSDDVPSLEIVKDCKVFWKKLLDAKLMSQRKYDNLTKAERGGLTSDDKARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIRKVKIVTLKSNLVSNFRKEFGFYKIREVNNYHHAHDAYLNAVVAKAILTKYPQLEPEFVYGDYPKYNSYKTRKSATEKLFFYSNIMNFFKTKVTLADGTVVVKDDIEVNNDTGEIVWDKKKHFATVRKVLSYPQVNIVKKTEVQTGGFSKESILAHGNSDKLIPRKTKDIYLDPKKYGGFDSPIVAYSVLVVADIKKGKAQKLKTVTELLGITIMERSRFEKNPSAFLESKGYLNIRTDKLIILPKYSLFELENGRRRLLASAGELQKGNELALPTQYMKFLYLASRYNELKGKPEEIEQKQEFVVQHVSYFDDILQIINDFSNRVILADANLEKINKLYQDNKENIPVDELANNIINLFTFTSLGAPAAFKFFDKIVDRKRYTSTKEVLNSTLIHQSITGLYETRIDLGKLGEG
>NZ_CP020449.2|WP_000653355.1|1588857_1589514_-|TIGR01906-family-membrane-protein
MKDKLLVVLTWIWIISLATLATIYIAWLIYPIEIQFLKLEKVVYLKAETIYYNFNKLMIYLTHPFISDLNMPSFPSSEDGLKHFADVKYLFTLAHGLFVILTFPVIYFLRHGWKQKSIFLYEGFFKIAIMLPIFIVVCAFLLGFDQFFTLFHEVLFPGDSTWQFNPLTDPVIWILPETFFLHCFIIFLLIYETITIILLIIGRKHLKLRKYKNKMQAL
>NZ_CP020449.2|WP_000323544.1|1589503_1590274_-|TIGR01457-family-HAD-type-hydrolase
MAYKGYLIDLDGTIYKGKSRIPAGERFIERLQEKGIPYMLVTNNTTRTPESVQEMLRGFNVETPLETIYTATMATVDYMNDMNRGKTAYVIGEEGLKKAIADAGYVEDTKNPAYVVVGLDWNVTYDKLATATLAIQNGALFIGTNPDLNIPTERGLLPGAGSLNALLEAATRIKPVFIGKPNAIIMNKALEILNIPRNQAVMVGDNYLTDIMAGINNDIDTLLVTTGFTTVEEVPDLPIQPSYVLASLDEWTFNEG
>NZ_CP020449.2|WP_000523795.1|1590274_1591012_-|acyl-ACP-thioesterase
MGLLYRETYEVPFYESDTNHYMKLPQLLALALQISAKQSLKLGIGDDIVFKRYGLVWVVTDYIIDIERLPKHAEKIVIETEAKAHNKLLCYRYFYIYGEDGQKIITISSAFVLMDFKTRKIHPVLDDITSIYQSQRIKKVIRGPKYHPIGDSKVKQYHVRYFDLDMNGHVNNSKYLEWMYDVLDLDFLSSHIPKKIDLKYIKEIQYGTDIKSHWYQDGLVTRHDIIGGDAIHAQARIEWQEKKED
>NZ_CP020449.2|WP_000914659.1|1591015_1592146_-|oxygen-independent-coproporphyrinogen-III-oxidase
MLKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDAYLQALIREFRSYDITELRTLYIGGGTPTSISAVQLDYLLTELIRDLNLNTLEEFTIEANPGDLTVDKIEVLQKSAVNRVSLGVQTFNDKHLKRIGRSHNEAQIYSTIDALKTAGFQNISIDLIYALPGQTMDDVRSNVAKALSLNIPHLSLYSLILEHHTVFMNKMRRGKLHLPTEDLEAEMFEYIISEMERNGFEHYEISNFTKPGFESRHNLMYWENVEYYGVGAGASGYLDGIRYRNRGPIQHYLKGVSEGNARLSEEVLSKNEMMEEELFLGLRKKEGVSIGKFEQKFGTSFEKRYGQIVQELQSDGLLKENNGFIQMTKKGLFLGDTVAEKFIVE
>NZ_CP020449.2|WP_001241816.1|1592238_1592619_-|hypothetical-protein
MRLWHQDIIELLPRQQLLGQHRECCALRGNGWGRKHETVDYVFRYSPYRLFAYHQLVMEEMMERGYRVSKEWLIAEYRGMKCPRYDTLNPVDLETPIYPEHNQDYLQECLWNLKAKGIDLPINKIK
>NZ_CP020449.2|WP_000159234.1|1592643_1593015_-|YbgA-family-protein
MTKEAELLWAKHKYLVLSKSQKIYLDIRQTLKSPNCTVLDVQSLIDQAVLLEESPSQVTNAYMHIWGYFKNKAERQEKEEFLTLLEKYRKTGYQRRKLLAFLKQLLAKYPNSYLQNSSIFEEE

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Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity
NZ_CP020449_1	1.4\|1582278\|28\|NZ_CP020449\|PILER-CR	1582278-1582305	28	MK448729	Streptococcus phage Javan31, complete genome	33404-33431	0	1.0
NZ_CP020449_1	1.4\|1582278\|28\|NZ_CP020449\|PILER-CR	1582278-1582305	28	MK448691	Streptococcus phage Javan17, complete genome	33404-33431	0	1.0
NZ_CP020449_1	1.4\|1582278\|28\|NZ_CP020449\|PILER-CR	1582278-1582305	28	MK448948	Streptococcus phage Javan46, complete genome	33348-33375	0	1.0
NZ_CP020449_1	1.4\|1582278\|28\|NZ_CP020449\|PILER-CR	1582278-1582305	28	MK448768	Streptococcus phage Javan47, complete genome	35056-35083	0	1.0
NZ_CP020449_1	1.6\|1582277\|30\|NZ_CP020449\|CRISPRCasFinder	1582277-1582306	30	MK448729	Streptococcus phage Javan31, complete genome	33403-33432	0	1.0
NZ_CP020449_1	1.6\|1582277\|30\|NZ_CP020449\|CRISPRCasFinder	1582277-1582306	30	MK448691	Streptococcus phage Javan17, complete genome	33403-33432	0	1.0
NZ_CP020449_1	1.6\|1582277\|30\|NZ_CP020449\|CRISPRCasFinder	1582277-1582306	30	MK448948	Streptococcus phage Javan46, complete genome	33347-33376	0	1.0
NZ_CP020449_1	1.6\|1582277\|30\|NZ_CP020449\|CRISPRCasFinder	1582277-1582306	30	MK448768	Streptococcus phage Javan47, complete genome	35055-35084	0	1.0
NZ_CP020449_1	1.7\|1582343\|30\|NZ_CP020449\|CRISPRCasFinder	1582343-1582372	30	NZ_LN906635	Lactobacillus reuteri plasmid p53608_1, complete genome, strain ATCC 53608	48868-48897	5	0.833
NZ_CP020449_1	1.2\|1582278\|39\|NZ_CP020449\|CRT	1582278-1582316	39	MK448729	Streptococcus phage Javan31, complete genome	33393-33431	6	0.846
NZ_CP020449_1	1.2\|1582278\|39\|NZ_CP020449\|CRT	1582278-1582316	39	MK448691	Streptococcus phage Javan17, complete genome	33393-33431	6	0.846
NZ_CP020449_1	1.2\|1582278\|39\|NZ_CP020449\|CRT	1582278-1582316	39	MK448948	Streptococcus phage Javan46, complete genome	33337-33375	6	0.846
NZ_CP020449_1	1.2\|1582278\|39\|NZ_CP020449\|CRT	1582278-1582316	39	MK448768	Streptococcus phage Javan47, complete genome	35045-35083	6	0.846
NZ_CP020449_1	1.5\|1582344\|28\|NZ_CP020449\|PILER-CR	1582344-1582371	28	NZ_CP024772	Bacillus thuringiensis LM1212 plasmid pLM1, complete sequence	40320-40347	6	0.786
NZ_CP020449_1	1.5\|1582344\|28\|NZ_CP020449\|PILER-CR	1582344-1582371	28	MF417965	Uncultured Caudovirales phage clone 3S_17, partial genome	3161-3188	6	0.786
NZ_CP020449_1	1.5\|1582344\|28\|NZ_CP020449\|PILER-CR	1582344-1582371	28	MH179477	Aeromonas phage 60AhydR15PP, complete genome	133528-133555	7	0.75
NZ_CP020449_1	1.5\|1582344\|28\|NZ_CP020449\|PILER-CR	1582344-1582371	28	NC_014635	Aeromonas phage phiAS4, complete genome	33181-33208	7	0.75
NZ_CP020449_1	1.5\|1582344\|28\|NZ_CP020449\|PILER-CR	1582344-1582371	28	MH179476	Aeromonas phage 50AhydR13PP, complete genome	93641-93668	7	0.75
NZ_CP020449_1	1.5\|1582344\|28\|NZ_CP020449\|PILER-CR	1582344-1582371	28	NC_020879	Aeromonas phage Aes012, complete genome	134557-134584	7	0.75
NZ_CP020449_1	1.5\|1582344\|28\|NZ_CP020449\|PILER-CR	1582344-1582371	28	NC_008208	Aeromonas phage 25, complete genome	134599-134626	7	0.75
NZ_CP020449_1	1.5\|1582344\|28\|NZ_CP020449\|PILER-CR	1582344-1582371	28	DQ529280	Aeromonas salmonicida bacteriophage 25, complete genome	134599-134626	7	0.75
NZ_CP020449_1	1.5\|1582344\|28\|NZ_CP020449\|PILER-CR	1582344-1582371	28	MF479730	Aeromonas phage AS-gz, complete genome	51898-51925	7	0.75
NZ_CP020449_1	1.5\|1582344\|28\|NZ_CP020449\|PILER-CR	1582344-1582371	28	JX306041	Stenotrophomonas phage IME13, complete genome	139584-139611	7	0.75
NZ_CP020449_1	1.5\|1582344\|28\|NZ_CP020449\|PILER-CR	1582344-1582371	28	NC_019543	Aeromonas phage Aes508, complete genome	133764-133791	7	0.75
NZ_CP020449_1	1.5\|1582344\|28\|NZ_CP020449\|PILER-CR	1582344-1582371	28	CP016197	Bacillus thuringiensis serovar coreanensis strain ST7 plasmid pST7-3, complete sequence	84107-84134	7	0.75
NZ_CP020449_1	1.7\|1582343\|30\|NZ_CP020449\|CRISPRCasFinder	1582343-1582372	30	NZ_CP024772	Bacillus thuringiensis LM1212 plasmid pLM1, complete sequence	40319-40348	7	0.767
NZ_CP020449_1	1.6\|1582277\|30\|NZ_CP020449\|CRISPRCasFinder	1582277-1582306	30	NZ_AP021845	Azospira sp. I09 plasmid pAZI09, complete sequence	290535-290564	8	0.733
NZ_CP020449_1	1.7\|1582343\|30\|NZ_CP020449\|CRISPRCasFinder	1582343-1582372	30	NC_020879	Aeromonas phage Aes012, complete genome	134556-134585	8	0.733
NZ_CP020449_1	1.7\|1582343\|30\|NZ_CP020449\|CRISPRCasFinder	1582343-1582372	30	MH179477	Aeromonas phage 60AhydR15PP, complete genome	133527-133556	8	0.733
NZ_CP020449_1	1.7\|1582343\|30\|NZ_CP020449\|CRISPRCasFinder	1582343-1582372	30	NC_008208	Aeromonas phage 25, complete genome	134598-134627	8	0.733
NZ_CP020449_1	1.7\|1582343\|30\|NZ_CP020449\|CRISPRCasFinder	1582343-1582372	30	NC_014635	Aeromonas phage phiAS4, complete genome	33180-33209	8	0.733
NZ_CP020449_1	1.7\|1582343\|30\|NZ_CP020449\|CRISPRCasFinder	1582343-1582372	30	DQ529280	Aeromonas salmonicida bacteriophage 25, complete genome	134598-134627	8	0.733
NZ_CP020449_1	1.7\|1582343\|30\|NZ_CP020449\|CRISPRCasFinder	1582343-1582372	30	MF479730	Aeromonas phage AS-gz, complete genome	51897-51926	8	0.733
NZ_CP020449_1	1.7\|1582343\|30\|NZ_CP020449\|CRISPRCasFinder	1582343-1582372	30	JX306041	Stenotrophomonas phage IME13, complete genome	139583-139612	8	0.733
NZ_CP020449_1	1.7\|1582343\|30\|NZ_CP020449\|CRISPRCasFinder	1582343-1582372	30	MH179476	Aeromonas phage 50AhydR13PP, complete genome	93640-93669	8	0.733
NZ_CP020449_1	1.7\|1582343\|30\|NZ_CP020449\|CRISPRCasFinder	1582343-1582372	30	NC_019543	Aeromonas phage Aes508, complete genome	133763-133792	8	0.733
NZ_CP020449_1	1.7\|1582343\|30\|NZ_CP020449\|CRISPRCasFinder	1582343-1582372	30	CP016197	Bacillus thuringiensis serovar coreanensis strain ST7 plasmid pST7-3, complete sequence	84106-84135	8	0.733
NZ_CP020449_1	1.7\|1582343\|30\|NZ_CP020449\|CRISPRCasFinder	1582343-1582372	30	MF417965	Uncultured Caudovirales phage clone 3S_17, partial genome	3160-3189	8	0.733

1. spacer 1.4|1582278|28|NZ_CP020449|PILER-CR matches to MK448729 (Streptococcus phage Javan31, complete genome) position: , mismatch: 0, identity: 1.0

ctgttcgctgtttgtgcgactattgtat	CRISPR spacer
ctgttcgctgtttgtgcgactattgtat	Protospacer
****************************

2. spacer 1.4|1582278|28|NZ_CP020449|PILER-CR matches to MK448691 (Streptococcus phage Javan17, complete genome) position: , mismatch: 0, identity: 1.0

ctgttcgctgtttgtgcgactattgtat	CRISPR spacer
ctgttcgctgtttgtgcgactattgtat	Protospacer
****************************

3. spacer 1.4|1582278|28|NZ_CP020449|PILER-CR matches to MK448948 (Streptococcus phage Javan46, complete genome) position: , mismatch: 0, identity: 1.0

ctgttcgctgtttgtgcgactattgtat	CRISPR spacer
ctgttcgctgtttgtgcgactattgtat	Protospacer
****************************

4. spacer 1.4|1582278|28|NZ_CP020449|PILER-CR matches to MK448768 (Streptococcus phage Javan47, complete genome) position: , mismatch: 0, identity: 1.0

ctgttcgctgtttgtgcgactattgtat	CRISPR spacer
ctgttcgctgtttgtgcgactattgtat	Protospacer
****************************

5. spacer 1.6|1582277|30|NZ_CP020449|CRISPRCasFinder matches to MK448729 (Streptococcus phage Javan31, complete genome) position: , mismatch: 0, identity: 1.0

actgttcgctgtttgtgcgactattgtatt	CRISPR spacer
actgttcgctgtttgtgcgactattgtatt	Protospacer
******************************

6. spacer 1.6|1582277|30|NZ_CP020449|CRISPRCasFinder matches to MK448691 (Streptococcus phage Javan17, complete genome) position: , mismatch: 0, identity: 1.0

actgttcgctgtttgtgcgactattgtatt	CRISPR spacer
actgttcgctgtttgtgcgactattgtatt	Protospacer
******************************

7. spacer 1.6|1582277|30|NZ_CP020449|CRISPRCasFinder matches to MK448948 (Streptococcus phage Javan46, complete genome) position: , mismatch: 0, identity: 1.0

actgttcgctgtttgtgcgactattgtatt	CRISPR spacer
actgttcgctgtttgtgcgactattgtatt	Protospacer
******************************

8. spacer 1.6|1582277|30|NZ_CP020449|CRISPRCasFinder matches to MK448768 (Streptococcus phage Javan47, complete genome) position: , mismatch: 0, identity: 1.0

actgttcgctgtttgtgcgactattgtatt	CRISPR spacer
actgttcgctgtttgtgcgactattgtatt	Protospacer
******************************

9. spacer 1.7|1582343|30|NZ_CP020449|CRISPRCasFinder matches to NZ_LN906635 (Lactobacillus reuteri plasmid p53608_1, complete genome, strain ATCC 53608) position: , mismatch: 5, identity: 0.833

catgt-tgttccatcatcaaataacattgca	CRISPR spacer
-atgtactttccatcatcaaataaagttgca	Protospacer
 **** . **************** .*****

10. spacer 1.2|1582278|39|NZ_CP020449|CRT matches to MK448729 (Streptococcus phage Javan31, complete genome) position: , mismatch: 6, identity: 0.846

ctgttcgctgtttgtgcgactattgtattgttttggaat-	CRISPR spacer
ctgttcgctgtttgtgcgactattgtatta-atcgcagta	Protospacer
*****************************.  *.* *.*

11. spacer 1.2|1582278|39|NZ_CP020449|CRT matches to MK448691 (Streptococcus phage Javan17, complete genome) position: , mismatch: 6, identity: 0.846

ctgttcgctgtttgtgcgactattgtattgttttggaat-	CRISPR spacer
ctgttcgctgtttgtgcgactattgtatta-atcgcagta	Protospacer
*****************************.  *.* *.*

12. spacer 1.2|1582278|39|NZ_CP020449|CRT matches to MK448948 (Streptococcus phage Javan46, complete genome) position: , mismatch: 6, identity: 0.846

ctgttcgctgtttgtgcgactattgtattgttttggaat-	CRISPR spacer
ctgttcgctgtttgtgcgactattgtatta-atcgcagta	Protospacer
*****************************.  *.* *.*

13. spacer 1.2|1582278|39|NZ_CP020449|CRT matches to MK448768 (Streptococcus phage Javan47, complete genome) position: , mismatch: 6, identity: 0.846

ctgttcgctgtttgtgcgactattgtattgttttggaat-	CRISPR spacer
ctgttcgctgtttgtgcgactattgtatta-atcgcagta	Protospacer
*****************************.  *.* *.*

14. spacer 1.5|1582344|28|NZ_CP020449|PILER-CR matches to NZ_CP024772 (Bacillus thuringiensis LM1212 plasmid pLM1, complete sequence) position: , mismatch: 6, identity: 0.786

atgttgttccatcatcaaataacattgc	CRISPR spacer
ctaatgttacatcatcaaataacatatc	Protospacer
 *. **** ****************  *

15. spacer 1.5|1582344|28|NZ_CP020449|PILER-CR matches to MF417965 (Uncultured Caudovirales phage clone 3S_17, partial genome) position: , mismatch: 6, identity: 0.786

atgttgttccatcatcaaataacattgc	CRISPR spacer
tttatgttccatcatcaaataccaatga	Protospacer
 *  ***************** ** **

16. spacer 1.5|1582344|28|NZ_CP020449|PILER-CR matches to MH179477 (Aeromonas phage 60AhydR15PP, complete genome) position: , mismatch: 7, identity: 0.75

atgttgttccatcatcaaataacattgc	CRISPR spacer
atgttgtttcatcatcaaataaagcata	Protospacer
********.************* ..

17. spacer 1.5|1582344|28|NZ_CP020449|PILER-CR matches to NC_014635 (Aeromonas phage phiAS4, complete genome) position: , mismatch: 7, identity: 0.75

atgttgttccatcatcaaataacattgc	CRISPR spacer
atgttgtttcatcatcaaataaagcata	Protospacer
********.************* ..

18. spacer 1.5|1582344|28|NZ_CP020449|PILER-CR matches to MH179476 (Aeromonas phage 50AhydR13PP, complete genome) position: , mismatch: 7, identity: 0.75

atgttgttccatcatcaaataacattgc	CRISPR spacer
atgttgtttcatcatcaaataaagcata	Protospacer
********.************* ..

19. spacer 1.5|1582344|28|NZ_CP020449|PILER-CR matches to NC_020879 (Aeromonas phage Aes012, complete genome) position: , mismatch: 7, identity: 0.75

atgttgttccatcatcaaataacattgc	CRISPR spacer
atgttgtttcatcatcaaataaagcata	Protospacer
********.************* ..

20. spacer 1.5|1582344|28|NZ_CP020449|PILER-CR matches to NC_008208 (Aeromonas phage 25, complete genome) position: , mismatch: 7, identity: 0.75

atgttgttccatcatcaaataacattgc	CRISPR spacer
atgttgtttcatcatcaaataaagcata	Protospacer
********.************* ..

21. spacer 1.5|1582344|28|NZ_CP020449|PILER-CR matches to DQ529280 (Aeromonas salmonicida bacteriophage 25, complete genome) position: , mismatch: 7, identity: 0.75

atgttgttccatcatcaaataacattgc	CRISPR spacer
atgttgtttcatcatcaaataaagcata	Protospacer
********.************* ..

22. spacer 1.5|1582344|28|NZ_CP020449|PILER-CR matches to MF479730 (Aeromonas phage AS-gz, complete genome) position: , mismatch: 7, identity: 0.75

atgttgttccatcatcaaataacattgc	CRISPR spacer
atgttgtttcatcatcaaataaagcata	Protospacer
********.************* ..

23. spacer 1.5|1582344|28|NZ_CP020449|PILER-CR matches to JX306041 (Stenotrophomonas phage IME13, complete genome) position: , mismatch: 7, identity: 0.75

atgttgttccatcatcaaataacattgc	CRISPR spacer
atgttgtttcatcatcaaataaagcata	Protospacer
********.************* ..

24. spacer 1.5|1582344|28|NZ_CP020449|PILER-CR matches to NC_019543 (Aeromonas phage Aes508, complete genome) position: , mismatch: 7, identity: 0.75

atgttgttccatcatcaaataacattgc	CRISPR spacer
atgttgtttcatcatcaaataaagcata	Protospacer
********.************* ..

25. spacer 1.5|1582344|28|NZ_CP020449|PILER-CR matches to CP016197 (Bacillus thuringiensis serovar coreanensis strain ST7 plasmid pST7-3, complete sequence) position: , mismatch: 7, identity: 0.75

atgttgttccatcatcaaataacattgc	CRISPR spacer
ccaatgttacatcatcaaataacatatc	Protospacer
 .. **** ****************  *

26. spacer 1.7|1582343|30|NZ_CP020449|CRISPRCasFinder matches to NZ_CP024772 (Bacillus thuringiensis LM1212 plasmid pLM1, complete sequence) position: , mismatch: 7, identity: 0.767

catgttgttccatcatcaaataacattgca	CRISPR spacer
actaatgttacatcatcaaataacatatca	Protospacer
  *. **** ****************  **

27. spacer 1.6|1582277|30|NZ_CP020449|CRISPRCasFinder matches to NZ_AP021845 (Azospira sp. I09 plasmid pAZI09, complete sequence) position: , mismatch: 8, identity: 0.733

actgttcgctgtttgtgcgactattgtatt-	CRISPR spacer
actgttcgctgtctgtgcgac-atgacgccg	Protospacer
************.******** ** .....

28. spacer 1.7|1582343|30|NZ_CP020449|CRISPRCasFinder matches to NC_020879 (Aeromonas phage Aes012, complete genome) position: , mismatch: 8, identity: 0.733

catgttgttccatcatcaaataacattgca	CRISPR spacer
tatgttgtttcatcatcaaataaagcataa	Protospacer
.********.************* ..   *

29. spacer 1.7|1582343|30|NZ_CP020449|CRISPRCasFinder matches to MH179477 (Aeromonas phage 60AhydR15PP, complete genome) position: , mismatch: 8, identity: 0.733

catgttgttccatcatcaaataacattgca	CRISPR spacer
tatgttgtttcatcatcaaataaagcataa	Protospacer
.********.************* ..   *

30. spacer 1.7|1582343|30|NZ_CP020449|CRISPRCasFinder matches to NC_008208 (Aeromonas phage 25, complete genome) position: , mismatch: 8, identity: 0.733

catgttgttccatcatcaaataacattgca	CRISPR spacer
tatgttgtttcatcatcaaataaagcataa	Protospacer
.********.************* ..   *

31. spacer 1.7|1582343|30|NZ_CP020449|CRISPRCasFinder matches to NC_014635 (Aeromonas phage phiAS4, complete genome) position: , mismatch: 8, identity: 0.733

catgttgttccatcatcaaataacattgca	CRISPR spacer
tatgttgtttcatcatcaaataaagcataa	Protospacer
.********.************* ..   *

32. spacer 1.7|1582343|30|NZ_CP020449|CRISPRCasFinder matches to DQ529280 (Aeromonas salmonicida bacteriophage 25, complete genome) position: , mismatch: 8, identity: 0.733

catgttgttccatcatcaaataacattgca	CRISPR spacer
tatgttgtttcatcatcaaataaagcataa	Protospacer
.********.************* ..   *

33. spacer 1.7|1582343|30|NZ_CP020449|CRISPRCasFinder matches to MF479730 (Aeromonas phage AS-gz, complete genome) position: , mismatch: 8, identity: 0.733

catgttgttccatcatcaaataacattgca	CRISPR spacer
tatgttgtttcatcatcaaataaagcataa	Protospacer
.********.************* ..   *

34. spacer 1.7|1582343|30|NZ_CP020449|CRISPRCasFinder matches to JX306041 (Stenotrophomonas phage IME13, complete genome) position: , mismatch: 8, identity: 0.733

catgttgttccatcatcaaataacattgca	CRISPR spacer
tatgttgtttcatcatcaaataaagcataa	Protospacer
.********.************* ..   *

35. spacer 1.7|1582343|30|NZ_CP020449|CRISPRCasFinder matches to MH179476 (Aeromonas phage 50AhydR13PP, complete genome) position: , mismatch: 8, identity: 0.733

catgttgttccatcatcaaataacattgca	CRISPR spacer
tatgttgtttcatcatcaaataaagcataa	Protospacer
.********.************* ..   *

36. spacer 1.7|1582343|30|NZ_CP020449|CRISPRCasFinder matches to NC_019543 (Aeromonas phage Aes508, complete genome) position: , mismatch: 8, identity: 0.733

catgttgttccatcatcaaataacattgca	CRISPR spacer
tatgttgtttcatcatcaaataaagcataa	Protospacer
.********.************* ..   *

37. spacer 1.7|1582343|30|NZ_CP020449|CRISPRCasFinder matches to CP016197 (Bacillus thuringiensis serovar coreanensis strain ST7 plasmid pST7-3, complete sequence) position: , mismatch: 8, identity: 0.733

catgttgttccatcatcaaataacattgca	CRISPR spacer
accaatgttacatcatcaaataacatatca	Protospacer
  .. **** ****************  **

38. spacer 1.7|1582343|30|NZ_CP020449|CRISPRCasFinder matches to MF417965 (Uncultured Caudovirales phage clone 3S_17, partial genome) position: , mismatch: 8, identity: 0.733

catgttgttccatcatcaaataacattgca	CRISPR spacer
ttttatgttccatcatcaaataccaatgag	Protospacer
. *  ***************** ** ** .

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

28911 : 36433

Bacillus_virus(50.0%)

transposase

The bacterium proteins that are colored denote the protein is present at specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin' and 'tRNA')

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_000041114.1\|28911_30246_-	aminopeptidase C	R4TV59	Phaeocystis_globosa_virus	1.1e-63	33.3
WP_041326139.1\|30335_31796_-\|transposase	IS1182 family transposase	A0A1X9I5T2	Streptococcus_phage	1.3e-208	76.5
WP_000174854.1\|31924_32746_-	ammonia-dependent NAD(+) synthetase	G3MA24	Bacillus_virus	7.7e-73	55.1
WP_000276183.1\|32742_34203_-	nicotinate phosphoribosyltransferase	G3MA18	Bacillus_virus	3.4e-100	42.3
WP_000272353.1\|34360_35275_-	thioredoxin-disulfide reductase	G3MA85	Bacillus_virus	2.1e-87	52.8
WP_000478229.1\|35343_35568_-	DUF4059 family protein	NA	NA	NA	NA
WP_000593862.1\|35689_36433_-	amino acid ABC transporter ATP-binding protein	G9BWD6	Planktothrix_phage	4.7e-29	38.1

DBSCAN-SWA_2

159324 : 177376

Streptococcus_phage(60.0%)

tRNA,integrase

attL 160674:160691|attR 173924:173941

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_041326067.1\|159324_160584_+\|tRNA	tyrosine--tRNA ligase	A0A1S6UA79	Serratia_phage	8.1e-74	41.2
160674:160691	attL	NA	NA	NA	NA
WP_041326065.1\|160848_161229_-	type II toxin-antitoxin system HicB family antitoxin	A0A1X9I5X0	Streptococcus_phage	4.7e-49	76.0
WP_003038349.1\|161265_161457_-	type II toxin-antitoxin system HicA family toxin	A0A1X9I5T5	Streptococcus_phage	1.7e-23	83.9
WP_041326062.1\|162235_162571_-	hypothetical protein	NA	NA	NA	NA
WP_024053142.1\|162570_163149_-	hypothetical protein	NA	NA	NA	NA
WP_024053143.1\|163196_163640_-	DUF1492 domain-containing protein	A0A1S5S8W3	Streptococcus_phage	7.9e-08	28.5
WP_041326850.1\|165079_166528_-	DNA primase family protein	Q9AZI5	Lactococcus_phage	8.8e-72	40.5
WP_041326060.1\|166610_167468_-	primase C-terminal domain-containing protein	A0A1X9I6L2	Streptococcus_phage	1.0e-123	74.7
WP_181970976.1\|167469_167616_-	hypothetical protein	A0A1X9I5Y0	Streptococcus_phage	8.3e-07	80.0
WP_041326058.1\|167602_167767_-	hypothetical protein	NA	NA	NA	NA
WP_041326056.1\|167763_168036_-	hypothetical protein	A0A1X9I5U9	Streptococcus_phage	3.2e-20	53.5
WP_024053150.1\|168038_168389_-	HTH domain-containing protein	A0A218MNC1	uncultured_virus	2.0e-06	36.8
WP_041326054.1\|168385_168622_-	hypothetical protein	NA	NA	NA	NA
WP_174721377.1\|168611_168788_-	hypothetical protein	NA	NA	NA	NA
WP_024053152.1\|168784_169312_-	hypothetical protein	NA	NA	NA	NA
WP_024053153.1\|169759_170392_-	Rha family transcriptional regulator	R9QNB1	Lactococcus_phage	1.0e-32	43.4
WP_041326052.1\|170382_171129_-	hypothetical protein	A0A0A7RW33	Clostridium_phage	7.3e-22	49.6
WP_024053155.1\|171145_171553_-	hypothetical protein	A0A1X9I5V7	Streptococcus_phage	1.4e-35	57.5
WP_003066809.1\|171567_171771_-	transcriptional regulator	NA	NA	NA	NA
WP_052471775.1\|171993_172584_+	helix-turn-helix transcriptional regulator	A0A1X9I723	Streptococcus_phage	7.6e-14	65.1
WP_041326046.1\|172777_173923_+\|integrase	site-specific integrase	A0A1X9I5Y5	Streptococcus_phage	2.3e-107	53.8
WP_000777421.1\|174621_175653_+	DNA/RNA non-specific endonuclease	NA	NA	NA	NA
173924:173941	attR	NA	NA	NA	NA
WP_000923270.1\|175863_176676_-	metal ABC transporter permease	NA	NA	NA	NA
WP_001269501.1\|176665_177376_-	metal ABC transporter ATP-binding protein	W6JKT0	Anomala_cuprea_entomopoxvirus	2.6e-08	24.9

DBSCAN-SWA_3

307837 : 316044

Synechococcus_phage(33.33%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_000783416.1\|307837_309142_-	LysM peptidoglycan-binding domain-containing protein	A0A2D1GNZ3	Streptomyces_phage	4.9e-05	40.5
WP_001045909.1\|309288_310188_-	peptidoglycan DD-metalloendopeptidase family protein	A0A221J6U0	Arthrobacter_phage	4.1e-19	45.9
WP_000166561.1\|310380_311928_-	bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase	Q58MG4	Prochlorococcus_phage	3.0e-78	50.2
WP_000780014.1\|311947_312700_-	GNAT family N-acetyltransferase	NA	NA	NA	NA
WP_000717515.1\|312722_313274_-	phosphoribosylglycinamide formyltransferase	M4QRX9	Synechococcus_phage	4.7e-26	35.8
WP_001293628.1\|313536_314562_-	phosphoribosylformylglycinamidine cyclo-ligase	R9TMC7	Synechococcus_phage	8.1e-64	42.7
WP_000638884.1\|314589_316044_-	amidophosphoribosyltransferase	A0A0M3SGR2	Mollivirus	1.7e-54	32.7

DBSCAN-SWA_4

383693 : 395772

Streptococcus_phage(44.44%)

transposase

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_017771557.1\|383693_385106_-\|transposase	IS1182 family transposase	A0A1X9I5T2	Streptococcus_phage	1.7e-208	76.5
WP_000264865.1\|385215_386325_-	DNA replication/repair protein RecF	NA	NA	NA	NA
WP_000384868.1\|386327_386681_-	S4 domain-containing protein YaaA	A0A1X9I5V8	Streptococcus_phage	9.4e-20	61.8
WP_000706187.1\|386919_388164_+	insulinase family protein	A0A1X9I714	Streptococcus_phage	6.3e-87	41.3
WP_000217756.1\|388165_389449_+	insulinase family protein	A0A2H4UVM3	Bodo_saltans_virus	2.2e-05	23.6
WP_000239225.1\|389571_390114_+	CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase	NA	NA	NA	NA
WP_000181757.1\|390113_390953_+	energy-coupling factor transporter ATPase	G9BWD6	Planktothrix_phage	3.5e-20	30.0
WP_000510606.1\|390928_391771_+	energy-coupling factor transporter ATPase	A0A2H4PQG7	Staphylococcus_phage	2.0e-15	28.5
WP_000359357.1\|391763_392558_+	energy-coupling factor transporter transmembrane protein EcfT	NA	NA	NA	NA
WP_000029072.1\|392773_393313_+	LysM peptidoglycan-binding domain-containing protein	A0A249XZV3	Enterococcus_phage	3.0e-17	46.4
WP_001042657.1\|393418_394123_+	transglycosylase SLT domain-containing protein	A0A2H4J6C5	uncultured_Caudovirales_phage	6.4e-28	65.3
WP_041326845.1\|394317_395772_+\|transposase	IS1182 family transposase	A0A1X9I5T2	Streptococcus_phage	1.3e-208	76.5

DBSCAN-SWA_5

410330 : 419822

Streptococcus_phage(81.82%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_001080836.1\|410330_410918_-	helix-turn-helix domain-containing protein	A0A1X9I5U6	Streptococcus_phage	4.0e-15	37.5
WP_000909285.1\|411072_411258_+	hypothetical protein	A0A1X9I5U7	Streptococcus_phage	6.2e-07	54.8
WP_001258609.1\|411273_411876_+	phage repressor protein	A0A1W6JPC3	Staphylococcus_phage	6.7e-42	46.7
WP_001021393.1\|412111_412750_+	Bro-N domain-containing protein	NA	NA	NA	NA
WP_000130289.1\|412848_413022_+	hypothetical protein	NA	NA	NA	NA
WP_001259478.1\|413110_413617_+	hypothetical protein	A0A1X9I5V7	Streptococcus_phage	4.5e-07	35.4
WP_000877499.1\|413693_414026_+	hypothetical protein	NA	NA	NA	NA
WP_001256559.1\|414022_414244_+	hypothetical protein	NA	NA	NA	NA
WP_001157003.1\|414246_414438_+	hypothetical protein	NA	NA	NA	NA
WP_001100460.1\|414778_415051_+	hypothetical protein	A0A1X9I5U9	Streptococcus_phage	1.8e-18	56.8
WP_001029296.1\|415051_415909_+	primase C-terminal domain-containing protein	A0A1X9I6L2	Streptococcus_phage	3.0e-120	72.9
WP_000656430.1\|415920_417315_+	hypothetical protein	W8CQP1	Croceibacter_phage	3.7e-43	33.7
WP_001069293.1\|417607_417865_+	hypothetical protein	NA	NA	NA	NA
WP_000694573.1\|417861_418035_+	hypothetical protein	A0A1X9I5U0	Streptococcus_phage	4.3e-10	56.1
WP_001258765.1\|418192_418750_+	hypothetical protein	A0A1X9I5U4	Streptococcus_phage	2.4e-30	59.8
WP_000891152.1\|418822_419311_+	hypothetical protein	A0A1X9I5V2	Streptococcus_phage	3.5e-49	59.3
WP_000038826.1\|419444_419822_+	DUF1492 domain-containing protein	Q7Y4J3	Streptococcus_phage	3.2e-10	37.9

DBSCAN-SWA_6

697495 : 723777

Streptococcus_phage(85.71%)

transposase,integrase

attL 683517:683531|attR 725572:725586

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
683517:683531	attL	NA	NA	NA	NA
WP_000181801.1\|697495_698719_+	glycine betaine/L-proline ABC transporter ATP-binding protein	G3M9Y6	Bacillus_virus	1.6e-29	37.0
WP_000429367.1\|698737_700465_+	ABC transporter permease/substrate binding protein	NA	NA	NA	NA
WP_000420682.1\|700983_701298_+	YdcP family protein	A0A1S5SF38	Streptococcus_phage	5.2e-54	100.0
WP_000985015.1\|701313_701700_+	YdcP family protein	A0A1S5SF96	Streptococcus_phage	3.0e-64	100.0
WP_000813488.1\|701728_703114_+	DNA translocase FtsK	A0A1S5SFB5	Streptococcus_phage	1.1e-265	100.0
WP_000879507.1\|703116_703269_+	hypothetical protein	NA	NA	NA	NA
WP_000398284.1\|703291_704497_+	replication initiation factor domain-containing protein	A0A1S5SEX3	Streptococcus_phage	2.8e-233	100.0
WP_001009056.1\|704539_704761_+	hypothetical protein	A0A1S5SEY0	Streptococcus_phage	4.3e-31	100.0
WP_000342539.1\|704877_705375_+	antirestriction protein ArdA	A0A1S5SF25	Streptococcus_phage	1.7e-91	100.0
WP_000506270.1\|705349_705856_+	conjugal transfer protein	A0A1S5SEX7	Streptococcus_phage	8.6e-91	100.0
WP_000331160.1\|705839_708287_+	ATP-binding protein	A0A1S5SF64	Streptococcus_phage	0.0e+00	100.0
WP_000804748.1\|708289_710467_+	membrane protein	A0A1S5SF30	Streptococcus_phage	0.0e+00	100.0
WP_000769868.1\|710463_711465_+	lysozyme family protein	A0A1S5SEZ8	Streptococcus_phage	5.0e-191	100.0
WP_001224318.1\|711461_712394_+	conjugal transfer protein	A0A1S5SF22	Streptococcus_phage	3.1e-171	99.4
WP_001791010.1\|712638_712755_+	tetracycline resistance determinant leader peptide	NA	NA	NA	NA
WP_000691736.1\|712770_714690_+	tetracycline resistance ribosomal protection protein Tet(M)	A0A1S5SF82	Streptococcus_phage	0.0e+00	99.5
WP_192573652.1\|714808_714976_+	cysteine-rich KTR domain-containing protein	D0R0F6	Streptococcus_phage	2.1e-14	68.0
WP_001814874.1\|715588_715672_+	23S rRNA methyltransferase attenuation leader peptide	NA	NA	NA	NA
WP_001038790.1\|715796_716534_+	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	E4ZFQ0	Streptococcus_phage	1.4e-134	99.6
WP_000576156.1\|716888_717443_+	recombinase family protein	A0A0F7LA37	Escherichia_phage	2.3e-36	52.1
WP_001240982.1\|717446_720365_+\|transposase	Tn3 family transposase	A0A1B0V7H9	Salmonella_phage	1.1e-169	36.1
WP_000804885.1\|721164_721587_+	sigma-70 family RNA polymerase sigma factor	A0A1S5SEW0	Streptococcus_phage	2.5e-72	100.0
WP_000857133.1\|721583_721814_+	helix-turn-helix domain-containing protein	A0A1S5SEX1	Streptococcus_phage	2.7e-36	100.0
WP_000814511.1\|722274_722478_+	excisionase	A0A1S5SF07	Streptococcus_phage	4.0e-31	100.0
WP_001291561.1\|722559_723777_+\|integrase	tyrosine-type recombinase/integrase	A0A1S5SEW7	Streptococcus_phage	3.7e-233	100.0
725572:725586	attR	NA	NA	NA	NA

DBSCAN-SWA_7

939728 : 955454

Streptococcus_phage(86.67%)

transposase

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_001185985.1\|939728_940493_+	ABC transporter ATP-binding protein	A0A2H4PQG7	Staphylococcus_phage	2.6e-14	24.8
WP_000178150.1\|940492_941203_+	ABC transporter ATP-binding protein	G9BWD6	Planktothrix_phage	4.7e-18	28.5
WP_001144242.1\|941221_941881_+	CBS domain-containing protein	M1NSC5	Streptococcus_phage	8.0e-65	56.5
WP_000715592.1\|941969_942605_+	dTMP kinase	M1PSC7	Streptococcus_phage	2.8e-67	61.1
WP_000364568.1\|942624_943488_+	DNA polymerase III subunit delta'	M1NSC1	Streptococcus_phage	2.5e-74	50.0
WP_000358198.1\|943518_943845_+	DNA replication initiation control protein YabA	M1PFV3	Streptococcus_phage	3.2e-30	61.1
WP_000425866.1\|943844_944714_+	16S rRNA (cytidine(1402)-2'-O)-methyltransferase	M1PLC5	Streptococcus_phage	4.1e-109	70.4
WP_017771557.1\|944855_946268_+\|transposase	IS1182 family transposase	A0A1X9I5T2	Streptococcus_phage	1.7e-208	76.5
WP_001292556.1\|946317_947595_-	group II intron reverse transcriptase/maturase	NA	NA	NA	NA
WP_000603271.1\|948408_949044_-	copper homeostasis protein CutC	NA	NA	NA	NA
WP_000158397.1\|949176_950268_+	3-phosphoserine/phosphohydroxythreonine transaminase	M1Q1P2	Streptococcus_phage	3.9e-165	78.0
WP_001198082.1\|950336_950885_+	GNAT family acetyltransferase	M1PSC3	Streptococcus_phage	1.4e-54	57.9
WP_000232154.1\|950946_952128_+	phosphoglycerate dehydrogenase	M1NSB9	Streptococcus_phage	9.1e-168	75.3
WP_000966769.1\|952183_952660_+	methylated-DNA--[protein]-cysteine S-methyltransferase	M1PFU9	Streptococcus_phage	1.6e-38	50.9
WP_000287943.1\|952661_953018_+	arsenate reductase family protein	M1PLC0	Streptococcus_phage	5.2e-42	72.6
WP_041326615.1\|953147_954605_+\|transposase	IS1182 family transposase	A0A1X9I5T2	Streptococcus_phage	1.3e-208	76.5
WP_000767486.1\|954626_955454_-	exodeoxyribonuclease III	M1PSC0	Streptococcus_phage	1.1e-124	78.0

DBSCAN-SWA_8

1178191 : 1242691

Bacillus_phage(42.86%)

transposase,protease,tRNA,integrase

attL 1178097:1178156|attR 1241463:1242779

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
1178097:1178156	attL	NA	NA	NA	NA
WP_000564846.1\|1178191_1179325_+\|transposase	ISAs1-like element IS1548 family transposase	NA	NA	NA	NA
WP_000452063.1\|1179489_1179870_+	DUF1149 family protein	NA	NA	NA	NA
WP_000761617.1\|1179869_1180718_+	DegV family protein	NA	NA	NA	NA
WP_001238956.1\|1180902_1182111_+\|tRNA	CCA tRNA nucleotidyltransferase	H7BUW3	unidentified_phage	3.3e-40	47.5
WP_001279321.1\|1182121_1183990_+	ABC-F family ATP-binding cassette domain-containing protein	A0A2K9L3Z8	Tupanvirus	1.6e-62	31.4
WP_000323499.1\|1184525_1186265_+	ABC transporter ATP-binding protein	W8CYL7	Bacillus_phage	1.3e-37	24.5
WP_001227931.1\|1186269_1188039_+	ABC transporter ATP-binding protein	W8CYL7	Bacillus_phage	2.0e-54	29.6
WP_000870955.1\|1188079_1188589_-	DUF308 domain-containing protein	NA	NA	NA	NA
WP_000200441.1\|1188756_1190106_+	NADP-specific glutamate dehydrogenase	NA	NA	NA	NA
WP_000594934.1\|1190175_1190586_+	peptide deformylase	A0A2I7QLT9	Vibrio_phage	1.0e-09	35.0
WP_000726912.1\|1190622_1192695_-	bifunctional metallophosphatase/5'-nucleotidase	NA	NA	NA	NA
WP_000431168.1\|1192963_1193404_+	MarR family transcriptional regulator	NA	NA	NA	NA
WP_000481823.1\|1193450_1195268_+	ABC transporter ATP-binding protein	W8CYL7	Bacillus_phage	2.4e-34	26.8
WP_000851088.1\|1195257_1197012_+	ABC transporter ATP-binding protein	W8CYL7	Bacillus_phage	3.0e-50	30.2
WP_000449636.1\|1197119_1197746_+	GTP pyrophosphokinase	NA	NA	NA	NA
WP_001264422.1\|1197747_1198434_+	response regulator transcription factor	NA	NA	NA	NA
WP_000489520.1\|1198433_1199663_+	HAMP domain-containing histidine kinase	NA	NA	NA	NA
WP_000659719.1\|1199778_1200657_+	mevalonate kinase	NA	NA	NA	NA
WP_094248914.1\|1200638_1201583_+	diphosphomevalonate decarboxylase	NA	NA	NA	NA
WP_000241520.1\|1201575_1202568_+	phosphomevalonate kinase	NA	NA	NA	NA
WP_000184894.1\|1202564_1203560_+	type 2 isopentenyl-diphosphate Delta-isomerase	NA	NA	NA	NA
WP_000594205.1\|1203627_1203846_+	DUF4649 family protein	NA	NA	NA	NA
WP_000206685.1\|1203891_1204746_-	glutathione S-transferase C-terminal domain-containing protein	NA	NA	NA	NA
WP_000833762.1\|1204846_1205761_-	diacylglycerol kinase family lipid kinase	NA	NA	NA	NA
WP_000910679.1\|1205847_1206504_-	hemolysin III family protein	NA	NA	NA	NA
WP_001039193.1\|1206496_1206946_-	DUF1836 domain-containing protein	NA	NA	NA	NA
WP_000161688.1\|1207037_1208321_-	hydroxymethylglutaryl-CoA reductase, degradative	NA	NA	NA	NA
WP_001244949.1\|1208322_1209600_-	hydroxymethylglutaryl-CoA synthase	NA	NA	NA	NA
WP_000158597.1\|1209648_1210488_+	thymidylate synthase	U5J9N5	Bacillus_phage	9.9e-84	53.6
WP_000166043.1\|1210567_1211062_+	dihydrofolate reductase	A0A0A8JB64	Ralstonia_phage	6.3e-22	35.3
WP_000918412.1\|1211279_1212506_+\|protease	ATP-dependent Clp protease ATP-binding subunit ClpX	G3M9Z9	Bacillus_virus	8.1e-135	60.0
WP_000796925.1\|1212516_1213113_+	YihA family ribosome biogenesis GTP-binding protein	NA	NA	NA	NA
WP_000140673.1\|1213093_1213723_-	TetR/AcrR family transcriptional regulator	NA	NA	NA	NA
WP_000120090.1\|1214475_1215126_+	HXXEE domain-containing protein	NA	NA	NA	NA
WP_000532330.1\|1216636_1217581_+	homocysteine S-methyltransferase	NA	NA	NA	NA
WP_000002804.1\|1217674_1219783_-\|protease	ATP-dependent Clp protease ATP-binding subunit	H6X3M6	Enterobacteria_phage	8.2e-119	37.4
WP_001287285.1\|1220216_1220717_+	50S ribosomal protein L10	NA	NA	NA	NA
WP_001196976.1\|1220780_1221146_+	50S ribosomal protein L7/L12	NA	NA	NA	NA
WP_000974945.1\|1221422_1225034_+	Eco57I restriction-modification methylase domain-containing protein	A0A1B1IUC6	uncultured_Mediterranean_phage	1.9e-06	24.3
WP_001129984.1\|1225047_1225539_+	hypothetical protein	NA	NA	NA	NA
WP_000884910.1\|1225590_1226796_+	hypothetical protein	NA	NA	NA	NA
WP_000857661.1\|1227030_1227345_+	hypothetical protein	NA	NA	NA	NA
WP_000161972.1\|1227344_1227848_+	hypothetical protein	NA	NA	NA	NA
WP_000581744.1\|1227911_1228421_+	hypothetical protein	NA	NA	NA	NA
WP_000031192.1\|1229170_1229737_+	replication protein	NA	NA	NA	NA
WP_000198709.1\|1229740_1229974_+	DUF3173 family protein	NA	NA	NA	NA
WP_001284550.1\|1229991_1231173_+\|integrase	site-specific integrase	NA	NA	NA	NA
WP_000248019.1\|1231374_1232610_-	hypothetical protein	NA	NA	NA	NA
WP_000007459.1\|1233025_1234840_+	3'-5' exoribonuclease	A0A0A7RWA3	Clostridium_phage	5.0e-32	31.9
WP_161800227.1\|1236160_1237318_+\|transposase	ISLre2 family transposase	NA	NA	NA	NA
WP_094248915.1\|1237854_1241307_+	C5a peptidase ScpA/B	A0A218KC60	Bacillus_phage	7.6e-05	39.4
WP_000564846.1\|1241557_1242691_+\|transposase	ISAs1-like element IS1548 family transposase	NA	NA	NA	NA
1241463:1242779	attR	NA	NA	NA	NA

DBSCAN-SWA_9

1925759 : 1982960

Streptococcus_phage(71.88%)

holin,portal,capsid,transposase,terminase,tail,integrase

attL 1942111:1942128|attR 1980650:1980667

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_000564843.1\|1925759_1926893_-\|transposase	ISAs1-like element IS1548 family transposase	NA	NA	NA	NA
WP_000078930.1\|1927062_1928268_-	rod shape-determining protein RodA	NA	NA	NA	NA
WP_017771557.1\|1928588_1930001_+\|transposase	IS1182 family transposase	A0A1X9I5T2	Streptococcus_phage	1.7e-208	76.5
WP_001292556.1\|1930050_1931328_-	group II intron reverse transcriptase/maturase	NA	NA	NA	NA
WP_000027835.1\|1932113_1932302_-	hypothetical protein	J7KIW4	Streptococcus_phage	3.3e-16	78.2
WP_000076708.1\|1932343_1932544_-	CsbD family protein	J7KJ36	Streptococcus_phage	8.4e-26	97.0
WP_000734169.1\|1933420_1934740_-	HAMP domain-containing histidine kinase	W8CYF6	Bacillus_phage	2.2e-05	25.9
WP_000699093.1\|1934736_1935390_-	response regulator transcription factor	W8CYM9	Bacillus_phage	8.1e-25	33.6
WP_000594351.1\|1935486_1936863_-	FtsX-like permease family protein	NA	NA	NA	NA
WP_000353149.1\|1936862_1937519_-	ABC transporter ATP-binding protein	G9BWD6	Planktothrix_phage	2.2e-22	31.8
WP_000594360.1\|1937528_1938806_-	ABC transporter permease	NA	NA	NA	NA
WP_000508795.1\|1939548_1939710_+	NINE protein	NA	NA	NA	NA
WP_001867107.1\|1939882_1940092_-\|transposase	transposase	A0A0C5AEA5	Paenibacillus_phage	1.4e-15	55.4
WP_001865698.1\|1940217_1940478_-\|transposase	IS3 family transposase	A0A0C5AEA5	Paenibacillus_phage	3.4e-11	43.0
WP_001122305.1\|1940499_1940700_-\|transposase	IS3 family transposase	NA	NA	NA	NA
WP_000809083.1\|1940861_1941089_-	helix-turn-helix domain-containing protein	NA	NA	NA	NA
WP_001881173.1\|1941265_1941532_+	hypothetical protein	Q77YW7	Streptococcus_phage	7.1e-20	65.2
WP_079218646.1\|1941534_1942149_+\|integrase	site-specific integrase	F8HGP4	Streptococcus_phage	9.2e-55	60.7
1942111:1942128	attL	NA	NA	NA	NA
WP_000965642.1\|1942248_1942428_-	hypothetical protein	J7KIW4	Streptococcus_phage	2.1e-20	86.2
WP_000356856.1\|1942844_1943015_+	hypothetical protein	A0A286QNA2	Streptococcus_phage	1.1e-07	63.8
WP_000076715.1\|1943055_1943256_-	CsbD family protein	J7KJ36	Streptococcus_phage	9.6e-22	87.9
WP_000812952.1\|1943359_1944331_-	Abi family protein	A0A059NT88	Lactococcus_phage	1.3e-74	42.6
WP_000236381.1\|1944663_1945383_-	CHAP domain-containing protein	A0A1U9WRD0	Streptococcus_virus	1.6e-58	72.1
WP_001001742.1\|1945384_1945720_-\|holin	phage holin	A0A1P8VVK6	Streptococcus_phage	6.8e-36	71.2
WP_000215499.1\|1945721_1946024_-	hypothetical protein	X2KT07	Streptococcus_phage	2.2e-25	59.6
WP_000698337.1\|1946036_1946249_-	hypothetical protein	J7KDP6	Streptococcus_phage	2.1e-14	60.0
WP_000494205.1\|1946223_1946553_-	DUF1366 domain-containing protein	J7KDI9	Streptococcus_phage	7.2e-22	54.3
WP_000536253.1\|1946578_1948585_-	hypothetical protein	J7KK23	Streptococcus_phage	3.6e-140	43.3
WP_000955555.1\|1948595_1952720_-\|tail	phage tail protein	J7KBT9	Streptococcus_phage	0.0e+00	75.6
WP_172581606.1\|1952720_1954196_-\|tail	phage tail family protein	J7KH53	Streptococcus_phage	2.9e-203	74.6
WP_000224695.1\|1954234_1956247_-	hypothetical protein	Q9F4J3	Streptococcus_phage	9.8e-13	58.9
WP_000916438.1\|1956246_1956618_-	DUF5361 domain-containing protein	A0A1P8BMT6	Lactococcus_phage	7.3e-31	54.0
WP_000858406.1\|1956632_1956878_-	hypothetical protein	A0A0B5A2F3	Streptococcus_phage	9.7e-16	55.3
WP_000226354.1\|1956877_1957435_-\|tail	tail protein	M1PKG8	Streptococcus_phage	3.4e-64	65.8
WP_000573598.1\|1957444_1957780_-	hypothetical protein	A0A1P8BMR5	Lactococcus_phage	9.8e-35	64.9
WP_000032787.1\|1957780_1958017_-	hypothetical protein	A0A1P8BMT2	Lactococcus_phage	2.5e-21	66.7
WP_000517229.1\|1958009_1958348_-	hypothetical protein	A0A0B5A086	Streptococcus_phage	1.4e-41	67.9
WP_000180800.1\|1958298_1958730_-	phage Gp19/Gp15/Gp42 family protein	A0A0B5A2F6	Streptococcus_phage	3.8e-47	70.1
WP_000640307.1\|1958743_1958959_-	hypothetical protein	M1PRX2	Streptococcus_phage	6.5e-08	52.9
WP_000841023.1\|1958955_1959858_-\|capsid	phage major capsid protein	A0A0B5A5W3	Streptococcus_phage	9.7e-146	86.0
WP_001288694.1\|1959860_1960325_-	DUF4355 domain-containing protein	A0A0B5A7G6	Streptococcus_phage	9.7e-41	51.3
WP_000256853.1\|1960405_1961821_-\|terminase	terminase	A0A0B5A091	Streptococcus_phage	6.3e-216	74.8
WP_000421991.1\|1961928_1962117_-	hypothetical protein	NA	NA	NA	NA
WP_000227333.1\|1962116_1963337_-	hypothetical protein	Q7Y4I9	Streptococcus_phage	6.6e-113	66.0
WP_001108669.1\|1963329_1964598_-\|portal	phage portal protein	A0A1X9I693	Streptococcus_phage	8.2e-191	76.2
WP_000725957.1\|1964594_1964951_-	hypothetical protein	A0A0B5A7G9	Streptococcus_phage	5.9e-22	49.6
WP_017646473.1\|1965102_1965480_-	HNH endonuclease	Q7Y4J1	Streptococcus_phage	2.1e-41	71.3
WP_000533373.1\|1966062_1966488_-	DUF1492 domain-containing protein	A0A0B5A564	Streptococcus_phage	6.4e-31	49.3
WP_000660741.1\|1966878_1967145_-	hypothetical protein	NA	NA	NA	NA
WP_001019148.1\|1967141_1967675_-	DUF1642 domain-containing protein	A0A097PAV5	Streptococcus_pyogenes_phage	1.6e-23	41.4
WP_000052302.1\|1967981_1968314_-	hypothetical protein	J7KDM7	Streptococcus_phage	2.6e-27	55.8
WP_000139629.1\|1968316_1968586_-	hypothetical protein	A7J287	Streptococcus_phage	1.3e-24	70.8
WP_000159369.1\|1968582_1968747_-	hypothetical protein	NA	NA	NA	NA
WP_000612734.1\|1968743_1969160_-	hypothetical protein	Q938M1	Temperate_phage	1.1e-32	58.5
WP_000763909.1\|1969294_1969597_-	DUF1599 domain-containing protein	J7KDH9	Streptococcus_phage	1.0e-30	74.7
WP_000129030.1\|1969608_1969956_-	hypothetical protein	J7KK12	Streptococcus_phage	2.2e-53	87.0
WP_000143298.1\|1969945_1970422_-	RusA family crossover junction endodeoxyribonuclease	J7KBT1	Streptococcus_phage	1.3e-59	98.2
WP_000165747.1\|1970411_1970615_-	hypothetical protein	J7KIX6	Streptococcus_phage	1.7e-26	96.8
WP_000609377.1\|1970618_1971035_-	single-stranded DNA-binding protein	Q938M7	Temperate_phage	1.5e-56	80.6
WP_001231960.1\|1971027_1971702_-	ERF family protein	Q938M8	Temperate_phage	7.6e-95	82.6
WP_000323859.1\|1971702_1972185_-	siphovirus Gp157 family protein	Q938M9	Temperate_phage	1.2e-44	92.5
WP_001001351.1\|1972186_1972348_-	hypothetical protein	J7KDI4	Streptococcus_phage	5.2e-18	84.9
WP_000229417.1\|1972350_1972605_-	hypothetical protein	J7KK18	Streptococcus_phage	1.1e-30	79.8
WP_000166038.1\|1972615_1972756_-	hypothetical protein	NA	NA	NA	NA
WP_000371783.1\|1972752_1972986_-	hypothetical protein	Q938N1	Temperate_phage	8.0e-36	94.8
WP_000774716.1\|1972966_1973827_-	phage replisome organizer N-terminal domain-containing protein	J7KBV5	Streptococcus_phage	5.6e-58	85.5
WP_000431178.1\|1973837_1974062_-	hypothetical protein	J7KBY5	Streptococcus_phage	1.7e-35	95.9
WP_000879943.1\|1974199_1974430_-	hypothetical protein	NA	NA	NA	NA
WP_001072517.1\|1974734_1975484_-	phage antirepressor Ant	F8HGQ0	Streptococcus_phage	6.5e-79	60.0
WP_001183891.1\|1975551_1975743_-	helix-turn-helix transcriptional regulator	NA	NA	NA	NA
WP_000573030.1\|1975794_1976601_+	DUF4393 domain-containing protein	A0A1S5SD60	Streptococcus_phage	1.9e-28	36.5
WP_000394501.1\|1976583_1976772_-	hypothetical protein	NA	NA	NA	NA
WP_001104154.1\|1976801_1976960_-	hypothetical protein	J7KH24	Streptococcus_phage	9.9e-22	92.3
WP_001107539.1\|1977018_1977243_+	DUF2188 domain-containing protein	E8ZD70	Streptococcus_phage	2.2e-22	67.6
WP_000392846.1\|1977239_1977383_-	hypothetical protein	NA	NA	NA	NA
WP_000550055.1\|1977743_1978541_+	XRE family transcriptional regulator	A0A1S5S8S8	Streptococcus_phage	2.6e-65	74.7
WP_000891067.1\|1978542_1979100_+	GTP pyrophosphokinase	Q0H269	Geobacillus_phage	1.6e-18	30.2
WP_000505363.1\|1979202_1979406_+	hypothetical protein	O34033	Streptococcus_phage	1.7e-10	48.4
WP_000264734.1\|1979528_1980608_+\|integrase	site-specific integrase	A0A1X9IGD8	Lactococcus_phage	3.2e-63	40.4
WP_001068667.1\|1980832_1981180_-	50S ribosomal protein L19	NA	NA	NA	NA
1980650:1980667	attR	NA	NA	NA	NA
WP_000595706.1\|1981763_1982960_-	voltage-gated chloride channel family protein	A0A1X9I5Z9	Streptococcus_phage	3.6e-103	54.5

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

Overview of predicted results

Overview of the results

Cas Category Instructions

Results visualization

1. NZ_CP020449

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Click the colored protein region to show detailed information

Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection