CRISPRimmunity

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Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Prophage number
NZ_CP022341	Bacillus velezensis strain 157 chromosome, complete genome	0 crisprs	csa3,DinG,casR,cas3,DEDDh,WYL	6
NZ_CP022342	Bacillus velezensis strain 157 plasmid p1-157 sequence	0 crisprs	NA	0
NZ_CP022343	Bacillus velezensis strain 157 plasmid p2-157 sequence	0 crisprs	NA	0

Results visualization

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CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

657283 : 667174

Synechococcus_phage(50.0%)

The bacterium proteins that are colored denote the protein is present at specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin' and 'tRNA')

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_022552701.1\|657283_658576_+	adenylosuccinate lyase	A0A2H4UUU6	Bodo_saltans_virus	4.5e-19	27.3
WP_014417101.1\|658651_659371_+	phosphoribosylaminoimidazolesuccinocarboxamide synthase	A0A0E3G7P8	Synechococcus_phage	1.7e-47	44.3
WP_014417102.1\|659370_659625_+	phosphoribosylformylglycinamidine synthase subunit PurS	M4QPE7	Synechococcus_phage	4.7e-05	33.3
WP_014417103.1\|659621_660305_+	phosphoribosylformylglycinamidine synthase subunit PurQ	NA	NA	NA	NA
WP_022552702.1\|660288_662517_+	phosphoribosylformylglycinamidine synthase subunit PurL	A6N228	Microbacterium_phage	1.0e-156	40.5
WP_014417105.1\|662492_663923_+	amidophosphoribosyltransferase	A0A0M3SGR2	Mollivirus	6.2e-54	32.7
WP_014417106.1\|664014_665055_+	phosphoribosylformylglycinamidine cyclo-ligase	A0A0E3F760	Synechococcus_phage	1.8e-63	41.1
WP_007408902.1\|665051_665639_+	phosphoribosylglycinamide formyltransferase	M4QRX9	Synechococcus_phage	1.3e-26	38.8
WP_014417107.1\|665635_667174_+	bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase	Q58MG4	Prochlorococcus_phage	3.3e-77	50.5

DBSCAN-SWA_2

1218049 : 1249921

Bacillus_phage(33.33%)

terminase,tail,portal,plate,holin

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_089072425.1\|1218049_1219186_+	tetratricopeptide repeat protein	A0A1P8CWN8	Bacillus_phage	2.2e-94	47.9
WP_022553496.1\|1219175_1219310_+	PhrA family phosphatase inhibitor	NA	NA	NA	NA
WP_022553495.1\|1219452_1220406_+	LysM peptidoglycan-binding domain-containing protein	A0A218KC88	Bacillus_phage	4.1e-62	69.3
WP_007610770.1\|1220443_1220821_-	PH domain-containing protein	A5GYQ0	Lactococcus_phage	1.5e-15	41.4
WP_022553494.1\|1220930_1221536_+	poly-gamma-glutamate hydrolase family protein	A0A0Y0AJU6	Bacillus_phage	6.1e-43	48.3
WP_014417517.1\|1221674_1222265_-	ImmA/IrrE family metallo-endopeptidase	A0A2H4JA43	uncultured_Caudovirales_phage	3.0e-39	51.7
WP_003154871.1\|1222412_1222751_-	helix-turn-helix transcriptional regulator	A0A2H4J6F9	uncultured_Caudovirales_phage	8.4e-18	45.8
WP_014417518.1\|1222941_1223121_+	hypothetical protein	NA	NA	NA	NA
WP_022553493.1\|1223110_1223938_+	phage-like element PBSX protein xkdB	S6BFM4	Thermus_phage	5.2e-21	51.0
WP_022553492.1\|1223837_1224638_+	ATP-binding protein	A6XMI1	Bacillus_virus	4.0e-58	45.3
WP_014417522.1\|1224902_1225244_+	hypothetical protein	NA	NA	NA	NA
WP_003154859.1\|1225233_1225437_+	hypothetical protein	A0A2H4J4M6	uncultured_Caudovirales_phage	5.4e-12	48.5
WP_012117362.1\|1225543_1226056_+	sigma-70 family RNA polymerase sigma factor	A0A0K2CNQ1	Brevibacillus_phage	2.9e-22	44.6
WP_022553491.1\|1226168_1226966_+\|terminase	PBSX phage terminase small subunit	A0A0S2MVB6	Bacillus_phage	2.3e-58	49.4
WP_022553490.1\|1226962_1228261_+\|terminase	PBSX family phage terminase large subunit	M4ZRM5	Bacillus_phage	2.7e-149	58.9
WP_089072483.1\|1228309_1229701_+\|portal	phage portal protein	A0A1B1P7C8	Bacillus_phage	1.4e-138	55.8
WP_003154849.1\|1229720_1230566_+\|portal	phage portal protein	A0A1B1P7E4	Bacillus_phage	2.5e-55	58.1
WP_022553488.1\|1230592_1231528_+	phage-like element PBSX protein xkdG	A0A1B1P7E3	Bacillus_phage	1.9e-104	62.8
WP_021494026.1\|1231544_1231928_+	DUF3199 family protein	NA	NA	NA	NA
WP_022553487.1\|1231924_1232281_+	DUF3599 family protein	NA	NA	NA	NA
WP_022553486.1\|1232277_1232781_+	HK97 gp10 family phage protein	NA	NA	NA	NA
WP_022553485.1\|1232777_1233224_+	hypothetical protein	NA	NA	NA	NA
WP_022553484.1\|1233220_1233430_+	hypothetical protein	NA	NA	NA	NA
WP_007610810.1\|1233429_1234827_+\|portal	phage portal protein	A0A0A7RTT5	Clostridium_phage	9.0e-82	40.4
WP_003154837.1\|1234828_1235272_+\|tail	phage tail tube protein	A0A0K2CNG3	Brevibacillus_phage	8.4e-26	45.2
WP_003154836.1\|1235345_1235792_+\|portal	phage portal protein	A0A0A7RTY8	Clostridium_phage	1.0e-10	35.8
WP_015239684.1\|1235833_1235986_+	hypothetical protein	NA	NA	NA	NA
WP_038461382.1\|1235973_1241100_+	transglycosylase SLT domain-containing protein	A0A1L2JY60	Aeribacillus_phage	3.4e-41	46.6
WP_022553482.1\|1241092_1241752_+	LysM peptidoglycan-binding domain-containing protein	G3MBQ1	Bacillus_virus	1.8e-08	45.3
WP_022553481.1\|1241765_1242743_+	hypothetical protein	A0A1L6BY20	Clostridium_phage	3.7e-34	30.6
WP_022553480.1\|1242742_1243009_+	DUF2577 domain-containing protein	S6C459	Thermus_phage	3.4e-06	37.5
WP_003154825.1\|1243112_1243538_+	DUF2634 domain-containing protein	A0A2H4J6K5	uncultured_Caudovirales_phage	1.5e-11	35.9
WP_022553478.1\|1243530_1244577_+\|plate	baseplate J/gp47 family protein	S6AVU3	Thermus_phage	1.0e-69	44.1
WP_029973069.1\|1244560_1245139_+	YmfQ family protein	A0A2H4J717	uncultured_Caudovirales_phage	2.4e-12	31.0
WP_003154822.1\|1245135_1245408_+	hypothetical protein	NA	NA	NA	NA
WP_022553476.1\|1245410_1247033_+	hypothetical protein	A0A1P8CWR7	Bacillus_phage	8.1e-42	39.7
WP_022553475.1\|1247045_1247417_+	hypothetical protein	NA	NA	NA	NA
WP_007610833.1\|1247421_1247619_+	XkdX family protein	A0A2H4JAA1	uncultured_Caudovirales_phage	7.3e-14	61.8
WP_022553474.1\|1247675_1248437_+	hypothetical protein	NA	NA	NA	NA
WP_022553473.1\|1248488_1248752_+	hypothetical protein	A0A2H4JD40	uncultured_Caudovirales_phage	1.5e-22	61.9
WP_003154813.1\|1248765_1249029_+\|holin	phage holin	A0A2H4J6M0	uncultured_Caudovirales_phage	2.3e-23	65.5
WP_007407257.1\|1249042_1249921_+	LysM peptidoglycan-binding domain-containing protein	Q9ZXD7	Bacillus_phage	3.7e-81	60.5

DBSCAN-SWA_3

1808138 : 1814352

Bacillus_phage(50.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_014417834.1\|1808138_1808531_+	class Ib ribonucleoside-diphosphate reductase assembly flavoprotein NrdI	G3MBF1	Bacillus_virus	5.0e-30	59.1
WP_007611605.1\|1808490_1810593_+	class 1b ribonucleoside-diphosphate reductase subunit alpha	U5PY35	Bacillus_phage	0.0e+00	85.7
WP_012117608.1\|1810610_1811600_+	class 1b ribonucleoside-diphosphate reductase subunit beta	F8WQ21	Bacillus_phage	1.1e-155	83.0
WP_014417835.1\|1811648_1812269_+	hypothetical protein	A0A127AYS1	Bacillus_phage	5.4e-47	48.4
WP_022553310.1\|1812318_1813077_-	N-acetylmuramoyl-L-alanine amidase	E5DV68	Deep-sea_thermophilic_phage	2.4e-52	50.0
WP_021493645.1\|1813110_1813335_-	hypothetical protein	NA	NA	NA	NA
WP_015417523.1\|1813383_1814352_+	stage V sporulation protein K	G3MAX6	Bacillus_virus	8.0e-53	40.9

DBSCAN-SWA_4

2108629 : 2115799

Bacillus_phage(100.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_022553207.1\|2108629_2108806_-	hypothetical protein	O64196	Bacillus_phage	6.5e-22	93.1
WP_038461826.1\|2109167_2109521_-	DUF3221 domain-containing protein	NA	NA	NA	NA
WP_021493939.1\|2110311_2110686_+	hypothetical protein	A0A1P8CWZ2	Bacillus_phage	8.1e-30	52.8
WP_021493940.1\|2110932_2111385_-	hypothetical protein	O64117	Bacillus_phage	6.3e-61	76.7
WP_022553203.1\|2111752_2112868_+	tetratricopeptide repeat protein	D6R410	Bacillus_phage	3.1e-93	48.1
WP_029974280.1\|2112864_2112987_+	phosphatase RapK inhibitor	NA	NA	NA	NA
WP_022553201.1\|2113530_2114547_+	hypothetical protein	NA	NA	NA	NA
WP_021493945.1\|2114607_2114967_-	hypothetical protein	O64028	Bacillus_phage	7.5e-33	61.4
WP_022553199.1\|2115574_2115799_-	helix-turn-helix transcriptional regulator	A0A1P8CWU2	Bacillus_phage	3.4e-23	80.3

DBSCAN-SWA_5

2272215 : 2278468

Staphylococcus_phage(66.67%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_007409428.1\|2272215_2272809_-	SMC-Scp complex subunit ScpB	A0A1B1IVT7	uncultured_Mediterranean_phage	2.6e-14	36.6
WP_007409427.1\|2272798_2273554_-	segregation/condensation protein A	A0A1B1IVW1	uncultured_Mediterranean_phage	2.9e-10	25.7
WP_003153376.1\|2273761_2273851_+	YjcZ family sporulation protein	NA	NA	NA	NA
WP_021494254.1\|2273938_2274460_+	DUF309 domain-containing protein	NA	NA	NA	NA
WP_003153373.1\|2274525_2274900_-	GNAT family N-acetyltransferase RibT	NA	NA	NA	NA
WP_003153372.1\|2275016_2275481_-	6,7-dimethyl-8-ribityllumazine synthase	A0A2H4PQS3	Staphylococcus_phage	1.2e-43	59.3
WP_014418281.1\|2275513_2276710_-	bifunctional 3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP cyclohydrolase II	A0A2H4PQS2	Staphylococcus_phage	2.1e-116	54.7
WP_021494255.1\|2276724_2277372_-	riboflavin synthase	A0A2H4PQS5	Staphylococcus_phage	2.6e-39	44.3
WP_022553020.1\|2277352_2278468_-	bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD	A0A2H4PQS8	Staphylococcus_phage	1.3e-54	36.3

DBSCAN-SWA_6

3665637 : 3714932

Staphylococcus_phage(16.67%)

protease,coat

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_003151043.1\|3665637_3666297_-\|protease	site-2 protease family protein	NA	NA	NA	NA
WP_003151042.1\|3666402_3666591_+	4-oxalocrotonate tautomerase	NA	NA	NA	NA
WP_007407650.1\|3666628_3667048_-	MarR family transcriptional regulator	NA	NA	NA	NA
WP_015240784.1\|3667431_3668811_+	amino acid permease	NA	NA	NA	NA
WP_007407653.1\|3668875_3669376_-	YwgA family protein	NA	NA	NA	NA
WP_022553940.1\|3669415_3670717_-	HD domain-containing protein	E3T4P8	Cafeteria_roenbergensis_virus	1.0e-23	29.5
WP_003151034.1\|3670876_3671101_-	DUF1450 domain-containing protein	NA	NA	NA	NA
WP_021494783.1\|3671303_3672077_+	RsfA family transcriptional regulator	NA	NA	NA	NA
WP_021494782.1\|3672376_3672652_+	PadR family transcriptional regulator	NA	NA	NA	NA
WP_022553942.1\|3672652_3673207_+	DUF1700 domain-containing protein	NA	NA	NA	NA
WP_022553943.1\|3673304_3674225_+	ATP-binding cassette domain-containing protein	A0A2H4PQG7	Staphylococcus_phage	1.3e-36	36.6
WP_022553944.1\|3674221_3675175_+	ABC transporter permease	NA	NA	NA	NA
WP_022553945.1\|3675164_3676001_-	hypothetical protein	NA	NA	NA	NA
WP_014419140.1\|3675991_3676789_-	hypothetical protein	NA	NA	NA	NA
WP_014721963.1\|3676757_3677681_-	hypothetical protein	NA	NA	NA	NA
WP_003151018.1\|3677729_3677909_-	hypothetical protein	NA	NA	NA	NA
WP_014419142.1\|3678061_3678925_-	lipoate--protein ligase family protein	NA	NA	NA	NA
WP_014419143.1\|3678971_3679871_-	LysR family transcriptional regulator	A0A2H4J8I9	uncultured_Caudovirales_phage	2.7e-07	40.2
WP_014419144.1\|3679986_3680964_+	putative sulfate exporter family transporter	NA	NA	NA	NA
WP_003151012.1\|3681000_3681972_-	phosphate acetyltransferase	NA	NA	NA	NA
WP_014419145.1\|3682228_3682993_+	heme-dependent peroxidase	NA	NA	NA	NA
WP_021494773.1\|3683112_3683892_+	SDR family oxidoreductase	NA	NA	NA	NA
WP_021494772.1\|3683908_3685108_-	pyridoxal phosphate-dependent aminotransferase	NA	NA	NA	NA
WP_089072468.1\|3685120_3686302_-	MFS transporter	NA	NA	NA	NA
WP_014419149.1\|3686298_3687717_-	ATP-grasp domain-containing protein	NA	NA	NA	NA
WP_014419150.1\|3687734_3688496_-	dihydroanticapsin 7-dehydrogenase	Q06VL0	Trichoplusia_ni_ascovirus	2.2e-21	30.1
WP_003151000.1\|3688492_3689203_-	cupin domain-containing protein	NA	NA	NA	NA
WP_014721966.1\|3689192_3689807_-	bacilysin biosynthesis protein BacA	NA	NA	NA	NA
WP_014419152.1\|3689968_3691207_-	MFS transporter	NA	NA	NA	NA
WP_014419153.1\|3691429_3692632_+	multidrug effflux MFS transporter	S4TR35	Salmonella_phage	7.1e-27	26.1
WP_012118711.1\|3692664_3694083_-	amino acid permease	NA	NA	NA	NA
WP_014419154.1\|3694107_3695790_-	M20/M25/M40 family metallo-hydrolase	NA	NA	NA	NA
WP_014419155.1\|3695861_3697409_-	L-glutamate gamma-semialdehyde dehydrogenase	NA	NA	NA	NA
WP_014419156.1\|3697616_3698903_-	Glu/Leu/Phe/Val dehydrogenase	NA	NA	NA	NA
WP_014419157.1\|3699134_3700070_-	sensor histidine kinase	W8CYF6	Bacillus_phage	9.8e-24	28.9
WP_014419158.1\|3700071_3700770_-	response regulator transcription factor	W8CYM9	Bacillus_phage	3.2e-35	35.8
WP_014419159.1\|3700961_3701828_-	DUF4097 family beta strand repeat protein	NA	NA	NA	NA
WP_014419160.1\|3701848_3702553_-	ABC transporter permease subunit	NA	NA	NA	NA
WP_025649399.1\|3702618_3703545_-	ABC transporter ATP-binding protein	A0A2H4PQG7	Staphylococcus_phage	1.1e-43	45.9
WP_003150986.1\|3703903_3704359_-\|coat	spore coat protein	NA	NA	NA	NA
WP_015418465.1\|3704355_3705204_-	dTDP-4-dehydrorhamnose reductase	A0A291LA50	Escherichia_phage	5.4e-37	37.3
WP_021494763.1\|3705224_3706172_-	dTDP-glucose 4,6-dehydratase	I7HTA3	Enterobacteria_phage	1.3e-68	42.9
WP_014419164.1\|3706174_3706912_-	NTP transferase domain-containing protein	G3MA50	Bacillus_virus	5.0e-47	42.7
WP_021494761.1\|3706939_3707944_-\|coat	spore coat protein	NA	NA	NA	NA
WP_089072469.1\|3707945_3708689_-\|coat	spore coat protein	NA	NA	NA	NA
WP_007614529.1\|3708678_3709800_-\|coat	spore coat protein	NA	NA	NA	NA
WP_014419167.1\|3709799_3710663_-	GNAT family N-acetyltransferase	NA	NA	NA	NA
WP_014419168.1\|3710663_3711833_-	DegT/DnrJ/EryC1/StrS family aminotransferase	NA	NA	NA	NA
WP_017418666.1\|3711855_3713280_-	CDP-glycerol glycerophosphotransferase family protein	NA	NA	NA	NA
WP_017418665.1\|3713284_3714055_-	glycosyltransferase family 2 protein	A0A0F7L2F7	uncultured_marine_virus	8.9e-07	28.0
WP_014419171.1\|3714335_3714932_+\|coat	spore coat protein GerQ	NA	NA	NA	NA

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage