CRISPRimmunity

Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Self targeting spacer number	Target MGE spacer number	Prophage number	Anti-CRISPR protein number
NZ_CP022385	Capnocytophaga sputigena strain KC1668 chromosome, complete genome	3 crisprs	DEDDh,cas3,WYL,PD-DExK,DinG,cas9	1	2	2	0

1. NZ_CP022385

CRISPR-Cas detection and classification

Crispr_ID: NZ_CP022385_1

• CRISPR_ID: NZ_CP022385_1
• CRISPR_location: 741406-741827
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 6 spacers

Consensus repeat of NZ_CP022385_1

Consensus_repeat	Method
TTACACTATTAGGAATA	PILER-CR

spacers of NZ_CP022385_1

>1.1|741423|52|NZ_CP022385|PILER-CR
ACTATAGATTTCAAAGATGTACAACCTGAAAAAGCACTCTCCCCTATACTTG
>1.2|741492|52|NZ_CP022385|PILER-CR
GCTATAGATTTCAAAGATGTACAAGCTTTAAAAGCATTTTTCTCAATACTTA
>1.3|741561|52|NZ_CP022385|PILER-CR
ACTATAGATTTCAAAGATGTACAACCTGAAAAAGCACTCTCCCCAATACTTG
>1.4|741630|49|NZ_CP022385|PILER-CR
GTAACAGATGCTAAAGAAGTACAACCATTAAAAGCTCCTGAAATATCTG
>1.5|741696|49|NZ_CP022385|PILER-CR
GTAACAGATGCTAAAGAAGTACAACCTCCAAAGGCTCCTGAAATTTTTG
>1.6|741762|49|NZ_CP022385|PILER-CR
GTAACAGATGCTAAAGAAGTACAACCAACAAAGACTCCTGAAATATCTG

CRISPR arrays and Neighbor proteins around NZ_CP022385_1

CRISPR arrays

The CRISPR arrays of NZ_CP022385_1

>merge|NZ_CP022385|1|741406-741827|PILER-CR
TTACACTATTAGGAATAACTATAGATTTCAAAGATGTACAACCTGAAAAAGCACTCTCCCCTATACTTGTTACACTATTAGGAATAGCTATAGATTTCAAAGATGTACAAGCTTTAAAAGCATTTTTCTCAATACTTATTACACTATTAGGAATAACTATAGATTTCAAAGATGTACAACCTGAAAAAGCACTCTCCCCAATACTTGTTACACTATTAGGAATAGTAACAGATGCTAAAGAAGTACAACCATTAAAAGCTCCTGAAATATCTGTTACGCTATTAGGAATAGTAACAGATGCTAAAGAAGTACAACCTCCAAAGGCTCCTGAAATTTTTGTTACACTATTAGGAATAGTAACAGATGCTAAAGAAGTACAACCAACAAAGACTCCTGAAATATCTGTTAAACTATTAGGAATA

>NZ_CP022385|1|1|741406-741827|PILER-CR
TTACACTATTAGGAATA	ACTATAGATTTCAAAGATGTACAACCTGAAAAAGCACTCTCCCCTATACTTG
TTACACTATTAGGAATA	GCTATAGATTTCAAAGATGTACAAGCTTTAAAAGCATTTTTCTCAATACTTA
TTACACTATTAGGAATA	ACTATAGATTTCAAAGATGTACAACCTGAAAAAGCACTCTCCCCAATACTTG
TTACACTATTAGGAATA	GTAACAGATGCTAAAGAAGTACAACCATTAAAAGCTCCTGAAATATCTG
TTACGCTATTAGGAATA	GTAACAGATGCTAAAGAAGTACAACCTCCAAAGGCTCCTGAAATTTTTG
TTACACTATTAGGAATA	GTAACAGATGCTAAAGAAGTACAACCAACAAAGACTCCTGAAATATCTG
TTAAACTATTAGGAATA

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
NZ_CP022385.1|WP_002678693.1|746452_747817_-|DNA-repair-protein-RadA	unknown	unknown	gnl|CDD|236994
NZ_CP022385.1|WP_002678881.1|737815_739756_+|TonB-dependent-receptor	unknown	unknown	gnl|CDD|227112
NZ_CP022385.1|WP_002678662.1|744592_745012_+|rRNA-maturation-RNase-YbeY	unknown	unknown	gnl|CDD|272871
NZ_CP022385.1|WP_002678665.1|730270_730933_+|RNA-methyltransferase	unknown	unknown	gnl|CDD|349965
NZ_CP022385.1|WP_002678914.1|732574_733363_+|hypothetical-protein	unknown	unknown	gnl|CDD|224495
NZ_CP022385.1|WP_002678807.1|751912_752845_-|malate-dehydrogenase	unknown	unknown	gnl|CDD|133424
NZ_CP022385.1|WP_002678816.1|739968_740496_-|hypothetical-protein	unknown	unknown	unknown
NZ_CP022385.1|WP_002678747.1|748724_749897_-|metallophosphoesterase	unknown	unknown	gnl|CDD|277368
NZ_CP022385.1|WP_002678860.1|730929_731478_+|protein-tyrosine-phosphatase	unknown	unknown	gnl|CDD|350378
NZ_CP022385.1|WP_002678857.1|750031_751165_+|glycosyl-hydrolase-family-5	unknown	unknown	gnl|CDD|376532
NZ_CP022385.1|WP_002678703.1|744373_744589_+|DUF2892-domain-containing-protein	unknown	unknown	gnl|CDD|378561
NZ_CP022385.1|WP_002678900.1|737031_737745_+|heme-binding-protein-HmuY	unknown	unknown	gnl|CDD|379448
NZ_CP022385.1|WP_002678688.1|735948_736932_+|polyprenyl-synthetase-family-protein	unknown	unknown	gnl|CDD|223220
NZ_CP022385.1|WP_050772795.1|743113_743761_-|collagen-like-protein	unknown	unknown	gnl|CDD|189968
NZ_CP022385.1|WP_002678648.1|731474_732572_+|Nif3-like-dinuclear-metal-center-hexameric-protein	unknown	unknown	gnl|CDD|376614
NZ_CP022385.1|WP_002678866.1|747825_748704_-|glucose-1-phosphate-thymidylyltransferase-RfbA	unknown	unknown	gnl|CDD|130274
NZ_CP022385.1|WP_002678663.1|751182_751776_-|M15-family-metallopeptidase	unknown	unknown	gnl|CDD|350617
NZ_CP022385.1|WP_002678683.1|745024_746440_-|oligosaccharide-flippase-family-protein	unknown	unknown	gnl|CDD|240533
NZ_CP022385.1|WP_002678748.1|733552_733924_+|ribosome-silencing-factor	unknown	unknown	gnl|CDD|376779
NZ_CP022385.1|WP_002678652.1|733928_735896_+|ATP-dependent-metallopeptidase-FtsH/Yme1/Tma-family-protein	unknown	unknown	gnl|CDD|273520

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
NZ_CP022385.1|WP_002678693.1|746452_747817_-|DNA-repair-protein-RadA	gnl|CDD|236994	PRK11823, PRK11823, DNA repair protein RadA; Provisional.	0
NZ_CP022385.1|WP_002678881.1|737815_739756_+|TonB-dependent-receptor	gnl|CDD|227112	COG4771, FepA, Outer membrane receptor for ferrienterochelin and colicins [Inorganic ion transport and metabolism].	2.72839e-61
NZ_CP022385.1|WP_002678662.1|744592_745012_+|rRNA-maturation-RNase-YbeY	gnl|CDD|272871	TIGR00043, Endoribonuclease_YbeY, rRNA maturation RNase YbeY. This metalloprotein family is represented by a single member sequence only in nearly every bacterium. Crystallography demonstrated metal-binding activity, possibly to nickel. It is a predicted to be a metallohydrolase, and more recently it was shown that mutants have a ribosomal RNA processing defect. [Protein synthesis, Other].	3.55228e-31
NZ_CP022385.1|WP_002678665.1|730270_730933_+|RNA-methyltransferase	gnl|CDD|349965	cd18092, SpoU-like_TrmH, SAM-dependent tRNA methylase related to TrmH. TrmH catalyzes the transfer of the methyl group from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of the ribose of the universally conserved guanosine 18 (G18) position in tRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.	3.10443e-76
NZ_CP022385.1|WP_002678914.1|732574_733363_+|hypothetical-protein	gnl|CDD|224495	COG1579, COG1579, Zn-ribbon protein, possibly nucleic acid-binding [General function prediction only].	1.88175e-12
NZ_CP022385.1|WP_002678807.1|751912_752845_-|malate-dehydrogenase	gnl|CDD|133424	cd01339, LDH-like_MDH, L-lactate dehydrogenase-like malate dehydrogenase proteins. Members of this subfamily have an LDH-like structure and an MDH enzymatic activity. Some members, like MJ0490 from Methanococcus jannaschii, exhibit both MDH and LDH activities. Tetrameric MDHs, including those from phototrophic bacteria, are more similar to LDHs than to other MDHs. LDH catalyzes the last step of glycolysis in which pyruvate is converted to L-lactate. MDH is one of the key enzymes in the citric acid cycle, facilitating both the conversion of malate to oxaloacetate and replenishing levels of oxalacetate by reductive carboxylation of pyruvate. The LDH-like MDHs are part of the NAD(P)-binding Rossmann fold superfamily, which includes a wide variety of protein families including the NAD(P)-binding domains of alcohol dehydrogenases, tyrosine-dependent oxidoreductases, glyceraldehyde-3-phosphate dehydrogenases, formate/glycerate dehydrogenases, siroheme synthases, 6-phosphogluconate dehydrogenases, aminoacid dehydrogenases, repressor rex, and NAD-binding potassium channel domains, among others.	1.2896e-150
NZ_CP022385.1|WP_002678688.1|735948_736932_+|polyprenyl-synthetase-family-protein	gnl|CDD|223220	COG0142, IspA, Geranylgeranyl pyrophosphate synthase [Coenzyme metabolism].	1.49566e-83
NZ_CP022385.1|WP_002678747.1|748724_749897_-|metallophosphoesterase	gnl|CDD|277368	cd07425, MPP_Shelphs, Shewanella-like phosphatases, metallophosphatase domain. This family includes bacterial, eukaryotic, and archeal proteins orthologous to the Shewanella cold-active protein-tyrosine phosphatase, CAPTPase. CAPTPase is an uncharacterized protein that belongs to the Shelph (Shewanella-like phosphatase) family of PPP (phosphoprotein phosphatases). The PPP family is one of two known protein phosphatase families specific for serine and threonine. In addition to Shelps, the PPP family also includes: PP1, PP2A, PP2B (calcineurin), PP4, PP5, PP6, PP7, Bsu1, RdgC, PrpE, PrpA/PrpB, and ApA4 hydrolase. The PPP catalytic domain is defined by three conserved motifs (-GDXHG-, -GDXVDRG- and -GNHE-). The PPP enzyme family is ancient with members found in all eukaryotes, and in most bacterial and archeal genomes. Dephosphorylation of phosphoserines and phosphothreonines on target proteins plays a central role in the regulation of many cellular processes. PPPs belong to the metallophosphatase (MPP) superfamily. MPPs are functionally diverse, but all share a conserved domain with an active site consisting of two metal ions (usually manganese, iron, or zinc) coordinated with octahedral geometry by a cage of histidine, aspartate, and asparagine residues. The MPP superfamily includes: Mre11/SbcD-like exonucleases, Dbr1-like RNA lariat debranching enzymes, YfcE-like phosphodiesterases, purple acid phosphatases (PAPs), YbbF-like UDP-2,3-diacylglucosamine hydrolases, and acid sphingomyelinases (ASMases). The conserved domain is a double beta-sheet sandwich with a di-metal active site made up of residues located at the C-terminal side of the sheets. This domain is thought to allow for productive metal coordination.	1.00056e-37
NZ_CP022385.1|WP_002678860.1|730929_731478_+|protein-tyrosine-phosphatase	gnl|CDD|350378	cd14529, TpbA-like, bacterial protein tyrosine and dual-specificity phosphatases related to Pseudomonas aeruginosa TpbA. This subfamily contains bacterial protein tyrosine phosphatases (PTPs) and dual-specificity phosphatases (DUSPs) related to Pseudomonas aeruginosa TpbA, a DUSP that negatively regulates biofilm formation by converting extracellular quorum sensing signals and to Mycobacterium tuberculosis PtpB, a PTP virulence factor that attenuates host immune defenses by interfering with signal transduction pathways in macrophages. PTPs (EC 3.1.3.48) catalyze the dephosphorylation of phosphotyrosine peptides, while DUSPs function as protein-serine/threonine phosphatases (EC 3.1.3.16) and PTPs.	3.58444e-27
NZ_CP022385.1|WP_002678857.1|750031_751165_+|glycosyl-hydrolase-family-5	gnl|CDD|376532	pfam01341, Glyco_hydro_6, Glycosyl hydrolases family 6.	9.6596e-46
NZ_CP022385.1|WP_002678703.1|744373_744589_+|DUF2892-domain-containing-protein	gnl|CDD|378561	pfam11127, DUF2892, Protein of unknown function (DUF2892). This family is conserved in bacteria. The function is not known.	4.42024e-09
NZ_CP022385.1|WP_002678900.1|737031_737745_+|heme-binding-protein-HmuY	gnl|CDD|379448	pfam14064, HmuY, HmuY protein. HmuY is a novel heme-binding protein that recruits heme from host carriers and delivers it to its cognate outer-membrane transporter, the TonB-dependent receptor HmuR. This family of proteins is found in bacteria. Proteins in this family are typically between 214 and 278 amino acids in length.	7.19819e-28
NZ_CP022385.1|WP_050772795.1|743113_743761_-|collagen-like-protein	gnl|CDD|189968	pfam01391, Collagen, Collagen triple helix repeat (20 copies). Members of this family belong to the collagen superfamily. Collagens are generally extracellular structural proteins involved in formation of connective tissue structure. The alignment contains 20 copies of the G-X-Y repeat that forms a triple helix. The first position of the repeat is glycine, the second and third positions can be any residue but are frequently proline and hydroxy-proline. Collagens are post translationally modified by proline hydroxylase to form the hydroxy-proline residues. Defective hydroxylation is the cause of scurvy. Some members of the collagen superfamily are not involved in connective tissue structure but share the same triple helical structure. The family includes bacterial collagen-like triple-helix repeat proteins.	3.972e-07
NZ_CP022385.1|WP_002678648.1|731474_732572_+|Nif3-like-dinuclear-metal-center-hexameric-protein	gnl|CDD|376614	pfam01784, NIF3, NIF3 (NGG1p interacting factor 3). This family contains several NIF3 (NGG1p interacting factor 3) protein homologs. NIF3 interacts with the yeast transcriptional coactivator NGG1p which is part of the ADA complex, the exact function of this interaction is unknown.	8.55709e-45
NZ_CP022385.1|WP_002678866.1|747825_748704_-|glucose-1-phosphate-thymidylyltransferase-RfbA	gnl|CDD|130274	TIGR01207, Glucose-1-phosphate_thymidylyltransferase_1, glucose-1-phosphate thymidylyltransferase, short form. Alternate name: dTDP-D-glucose synthase homotetramer This model describes a tightly conserved but broadly distributed subfamily (here designated as short form) of known and putative bacterial glucose-1-phosphate thymidylyltransferases. It is well characterized in several species as the first of four enzymes involved in the biosynthesis of dTDP-L-rhamnose, a cell wall constituent and a feedback inhibitor of the enzyme. [Cell envelope, Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides].	0
NZ_CP022385.1|WP_002678663.1|751182_751776_-|M15-family-metallopeptidase	gnl|CDD|350617	cd14840, D-Ala-D-Ala_dipeptidase_Aad, D-Ala-D-Ala dipeptidase (includes Lactobacillus plantarum Aad peptidase). D-Ala-D-Ala dipeptidase (also known as D-alanyl-D-alanine dipeptidase vanX; VanX; EC 3.4.13.22) is a Zn2+-dependent enzyme that mediates resistance to the antibiotic vancomycin in Enterococci and other bacteria (both Gram-positive and Gram-negative). It is part of a gene cluster that affects cell-wall biosynthesis. The operon triggers the termination of peptidoglycan precursors by D-Ala-(R)-lactate instead of D-Ala-D-Ala dipeptides. The enzyme is stereospecific, as L-Ala-L-Ala, D-Ala-L-Ala and L-Ala-D-Ala are not substrates. This subfamily includes Lactobacillus Aad peptidase and belongs in the MEROPS peptidase family M15, subfamily D.	2.95724e-96
NZ_CP022385.1|WP_002678683.1|745024_746440_-|oligosaccharide-flippase-family-protein	gnl|CDD|240533	cd13128, MATE_Wzx_like, Wzx, a subfamily of the multidrug and toxic compound extrusion (MATE)-like proteins. Escherichia coli Wzx and related proteins from other gram-negative bacteria are thought to act as flippases, assisting in the membrane translocation of lipopolysaccharides including those containing O-antigens. Proteins from the MATE family are involved in exporting metabolites across the cell membrane and are often responsible for multidrug resistance (MDR).	2.34123e-28
NZ_CP022385.1|WP_002678748.1|733552_733924_+|ribosome-silencing-factor	gnl|CDD|376779	pfam02410, RsfS, Ribosomal silencing factor during starvation. This family is expressed by almost all bacterial and eukaryotic genomes but not by archaea. Its function is to down-regulate protein synthesis under conditions of nutrient shortage, and it does this by binding to protein L14 of the large ribosomal subunit, thus acting as a ribosomal silencing factor (RsfS) by blocking the joining of the ribosomal subunits. This family is structurally homologous to nucleotidyltransferases.	1.11551e-39
NZ_CP022385.1|WP_002678652.1|733928_735896_+|ATP-dependent-metallopeptidase-FtsH/Yme1/Tma-family-protein	gnl|CDD|273520	TIGR01241, ATP-dependent_zinc_metalloprotease_FtsH, ATP-dependent metalloprotease FtsH. HflB(FtsH) is a pleiotropic protein required for correct cell division in bacteria. It has ATP-dependent zinc metalloprotease activity. It was formerly designated cell division protein FtsH. [Cellular processes, Cell division, Protein fate, Degradation of proteins, peptides, and glycopeptides].	0

Protein sequences

>NZ_CP022385.1|WP_002678816.1|739968_740496_-|hypothetical-protein
MKVILIQGKANTGKTTLCNRIDEWLLNNRIYELQQLINNRKNGQNFSIKEITKIEVIKQILPNNDFYAVYDIQFSIGGNKRVIINTLSDYNTITEFVNFYNNNKNKGYDIFITTIRDEGRPKEVIEEFCKEELKKINPPTQTKEIIDLNKNPITNVVESYAMLYYEFIPLFEKLI
>NZ_CP022385.1|WP_002678881.1|737815_739756_+|TonB-dependent-receptor
MQKFYIAAFIFLYSSLIFAQNDTLQNDLINLKEVIVTATRAQKNLKNVPITVQVVTAEGIRKSQAINFQSFLETEFAGINFTYNGGMPNINMMGFGGKYVLFLIDGERMAGETFDNIDYNRINLEDIERIEIIKGASSSLYGSNALGGVINIITKNAKKSFEGDLSYQYETIESHRANIGIGSKQRWGNIRLTSFYNFREPYLLKDREPLITYKNGVAEPSPKSELNIAGFTTYGVTPKLSFNLSPKTDLTLTPNYYFSERNQGDRDAERKRERYYNYTMSAKLNTALTESKKLSLSGAFDRYDKFNYFPLLKEKEKNYENTLARAGAQYNQSLWTKHSLVAGAEIFSDELLSFRFNETGTEAKENAQNYTLFTQQEWTLSPAFTLVTGVRVDYHSLFKEHFTYRLSGMLKVESFTFRGGFSSGFRSPTLKELYTNWFHPWGGGFQIIGNKDLKPESSNNFNFSVDFDSPKVNITAMTQLSLMKDKIDYRWTVASDTIRYINFKDKTQIISSELAATYRPTKALRFKASYAYYYISNSANENRPHTLTLKAEYIPKHNALYIPNIVFSGKYVSATRIHETDTSNNQLYTYYEPYSIWQLQVFSKLPYHLTLTAGIDNLFNYITPTTSFYSSISKGRTYSLGLKWNY
>NZ_CP022385.1|WP_002678900.1|737031_737745_+|heme-binding-protein-HmuY
MRLQFLAPAIIAGLMMFTTSCSKDDKKTEAPKLPYKEQKVDASSYDKWVYFSFENGVVTSTTATPTTTDWDIAFNRYSVRTNSGTSGSGNGGALTTNETDWDKVATASSTASFTVDGSIYVFERGQNNQGGLGTTNASRVISGAHGENFWNMISRMPNVAKIDKSSIVHNNGWLTMDYKPNGNQLAPSYTYNNWVYIVKTPAGKFVKIQLTDYKNAKDETGYITFKYQIANDKNEFK
>NZ_CP022385.1|WP_002678688.1|735948_736932_+|polyprenyl-synthetase-family-protein
MKITAQIKEPIREEMELFEQKFRNSMSSKVALLNRITYYIVNRKGKQMRPMFVFLVAKMLSDDSKVSERTYRGASVIELIHTATLVHDDVVDDSNKRRGFFSINALWKNKIAVLVGDYLLSKGLLLSIDNGDFDLLRIISVAVREMSEGELLQIEKARRLDIVEDVYYEIIRQKTATLIAACCAMGACSVQPEQPELIEKMRLFGEYIGMAFQIKDDLFDYTEDAIGKPTGIDIKEQKMTLPLIYVLNTCTEKEKQWLINSVKKYNKDKKRVKEVISFVKTHNGLEYATAKMKEFQQKALNILNDFPASSYKEALTLMVNYVIDRKI
>NZ_CP022385.1|WP_002678652.1|733928_735896_+|ATP-dependent-metallopeptidase-FtsH/Yme1/Tma-family-protein
MSDNKSNGNRFSAYWLYGVLLLILIGFNYYSGASFWSQPKEIPQSKFEEYLSNGDVAKIIILNRKEANVYLTEDALKKEEHKEVKPTGNSLMQGANAGEVPQYRFELGDLSNFENRFDQIVKDNNLSTTRENKTQQNVIGDLLFTLLPIIVVIGLWIFVMRRMAGAGGPGGIFSIGKSKARMFDEKKETRVTFQDVAGLEGAKEEVQEIVDFLKNPDKYTSLGGKIPRGALLVGPPGTGKTLLAKAVAGEAQVPFFSLSGSDFVEMFVGVGASRVRDLFKQAKEKSPSIIFIDEIDAIGRARGKNAMTGANDERENTLNQLLTEMDGFGSHTNVIVLAATNRADILDKALMRAGRFDRQIYVELPNLNERKQIFQVHLRPIKTAETLDIDFLAKQTPGFSGADIANVCNEAALIAARKGKTAVSKQEFLDAVDRIVGGLEKKTKILTPEEREAIAFHEAGHATVSWLLEHAAPLVKVTIVPRGQSLGAAWYLPEERQIVRTEQILDEMCAALGGRAAEKVVFNKISTGALSDLEKVTKQARAMVTIYGLNEKIGNLTYYDSAQSDYSFTKPYSEKTAHLIDEEISKIIEEQYQRAIDILTENKDKLTTLANLLLDREVIFRDDLENIFGKRKFKDPHNSNEEETPLKTENTSPTE
>NZ_CP022385.1|WP_002678748.1|733552_733924_+|ribosome-silencing-factor
MNKEISSNQLISNIIAGIEKVKGTDITIMDLREVENTVCDYFILCNGSSNTQVSAISGAIQKMVSQADGHQHPWHVEGEANAEWILIDYVDVVVHIFQTKVREHYDLEGLWGDAKFINLETNY
>NZ_CP022385.1|WP_002678914.1|732574_733363_+|hypothetical-protein
MAQKVDITVEEKLRALYDLQLIDSKIDELQYTRGELPLEVSDLEDEVEGLKKRHAKFKEELDALQAGIAEKKATIEEAKALIKKYTTQQKNVRNNREYTSLSKEIEFQELEVQLAEKRIKEGKAQVDQKKEAIKQVDERKAQRDAHLKHKKAELNSVLAETEKEEAFLKEKAEEYAAKIEDRLLKAYQRIRSSVMNKLAVVPIQRGASGGSFFTIPPQVQVEIASRKKIITDEHSGRILVDAALAEEEKEKMEALFKSFKAS
>NZ_CP022385.1|WP_002678648.1|731474_732572_+|Nif3-like-dinuclear-metal-center-hexameric-protein
MKITDIIQELEKLAPLQYAEGFDNVGLLVGDANAEVKGVLITLDTLEAVVDEAIAKKCNLIVSFHPIIFSGLKSLTGKNYVERVVMKAIQHQIAIYSMHTALDNQFLGVNASICNRLELRNRRILIPQPHTIQKLITYVPKSDAENLRKALFAAGAGNIGNYAECSFNLEGKGTYKGNEDSHPTIGEPNVFHTEDETQIGVIFPKHLQRQILQALRQNHPYEEVAFEIYTLENEHQHIGMGMIGELNKSMPEKDFLAYLKERMQVSVVRHSALLGKDVKKVAVLGGSGAFAIENAKRAKADVYITADLKYHEFFKAEGQILLADIGHFESEQYIKSLLFDYLSKIFPTFALSISNVDTNPIKYYS
>NZ_CP022385.1|WP_002678860.1|730929_731478_+|protein-tyrosine-phosphatase
MKKTLLICFILFALLPLWSQHKATLIHADANLYKVDSLLYRSEQLVTEDKAIIKNIPIKSIVNLRYFTRSGDKKIFNASDNIKLINHPLLTWRIKAPEIAQTLKIIREHQKQGAVLIHCYHGADRTGIMVAMYRIIYHNWTIEQAKKEMLNGPYGYHSVWKNLEALFTESTVKEVRKHLGMQ
>NZ_CP022385.1|WP_002678665.1|730270_730933_+|RNA-methyltransferase
MNKKLLSYLERFITEERKERFLEIIVQRTNHFTVAMEDVFQMHNTSAVVRTCEVFGVQQAHSIEGRFGKRLDAKIAMGAQKWVDVFRYNDTQSCIDTLRTQGYQIVATTPHKNAYFLNDFDITKKSAFFFGTEKEGLSAQVLSQADTFLKIPMVGFTESLNISVAVAIVLQQLTDKLRRSEVAWQLSDEERLNTLIHWTKQSIRNVDDVLTRYEEIKNTL
>NZ_CP022385.1|WP_050772795.1|743113_743761_-|collagen-like-protein
MKKMFFIAVAMFAMLTNVSCGKETITERIEVRKGNLIHSGYGVPSASLGEIGDYYIDLQNIHLYGAKTSQGWGTPINLKGEKGDRGEKGFMGWQGDKGDKGDRGQKGDQGEKGDRGQKGDQGEKGDQGEKGDRGEKGDKGDKGEKGEIGQKGDKGASTGEKGDKGKDGARIYGGTSVPNPSLGVEGDWYIDTLNKKLYGPKTNSGWGQGVSIFNN
>NZ_CP022385.1|WP_002678703.1|744373_744589_+|DUF2892-domain-containing-protein
MKRNISTLDKNIRLLIIVITAILGYFNEFSITIASVLSGVSILLLVTILINFSPIYALLGISTYKPKEKNS
>NZ_CP022385.1|WP_002678662.1|744592_745012_+|rRNA-maturation-RNase-YbeY
MTSFFNETPFVFPYNKNSVKRWVKSVAKSEGKETGNINYIFCDDEYLHKINVEYLQHDTLTDIITFDYTEEKVLHSDIYISVERVKENAEIFKVPFQRELLRVLAHGLLHLCGYKDKTPKDSALMRQKEEEMMLLFDQL
>NZ_CP022385.1|WP_002678683.1|745024_746440_-|oligosaccharide-flippase-family-protein
MLRKLFKDTIIYGIATVLPRVLTLLLTRLYVNKLDTADFGIYSGLFVYLILGNVLLSYGMETAFFRFMNKGEQKKKVQSTALTSLTISSLVFLLLAYLLRYAIASWLNYDVQYIVFAIYILVLDALVVIPFAWLRNKGKAKLYAMVKIGNTALNLALNIYYLNYLSAEKLATNGGVYYIFLANVIASLATFIVLLPIYLKIRFKFYYPLWKEMMIYAFPVLLAGIAFAVNEGFDRVFLRMLLPADTADGTIGIYSACYKMGVFMNLFVTAYKLGIEPFFFSSSQDKNAPKTYARITEYFIVCGGFILLFITVFTDVFKLILIPNKAYWDALWIVPIILLANLCLGIYHSLSVWYKVTDRTSFGAIVSLIGMAITVVFNFALIPWLSYKGAALATLITYAAMMSISYYYGQKYYPVPYKKRKIKIFLGMSVLFSFINFYAFGNSIYIGILFLLIYGYLASFAIKLKALRSKA
>NZ_CP022385.1|WP_002678693.1|746452_747817_-|DNA-repair-protein-RadA
MAKLKTAYFCQNCGAQYSKWQGQCYTCKQWNTIVEEVVQKDTAPAWQEKESPIKNKAPKYIPISQIDTQQEPRLNSNNHELNNVLGGGIVPGSVTLLGGEPGIGKSTLLLQIALNLPYRTLYVSGEESEKQIKMRADRIPHKLDNCFILTETKTQNIFQQIKELLPEIVIIDSIQTLQSDYIEASAGSISQIRECTSELIKFAKTTHTPVILIGHITKDGQIAGPKILEHMVDTVLQFEGDRNHVYRILRSLKNRFGSTSELGIYEMLSNGLREVNNPSEILISKTDETISGTSIAATLEGMRPLMIEIQALVSTAVYGTPQRSTTGFNAKRLNMLLAVLEKRAGFRLGAKDVFLNITGGITIDDPATDLGVAMAILSSNEDIAIEKNVCFAGEVGLGGEIRPVQRVEQRITEAEKLGFNTIFVSKYNKIALKNTKIKVITVAKIEDAIQELFG
>NZ_CP022385.1|WP_002678866.1|747825_748704_-|glucose-1-phosphate-thymidylyltransferase-RfbA
MKGIILAGGSGTRLYPITKGVSKQLLPIYDKPMIYYPLSVLMLSGIREILVISTPQDLPGFERLLGDGSDFGIRLSYAEQPSPDGLAQAFIIGEEFIGDDDVCLVLGDNIFYGQSFSKMLSQAVENVTKERKATVFGYYVKDPERYGVAEFDNAGNVLSIEEKPAHPKSNYAVVGLYFYPNKVVKVAKNIKPSARGELEITTVNQEFLNDGELKVQLLGRGFAWLDTGTHDSLSEASNFVETLEKRQGLKISCLEEIAYRKGWITAEKLQELAKPMLKNQYGQYLLQITSNR
>NZ_CP022385.1|WP_002678747.1|748724_749897_-|metallophosphoesterase
MKKTYITFLAFLCCFVLGNNWLQAQELVPILSQARLHKDNSKSGFDGPYIFYTDKGIVVKQVSEKKGVVAPSIQTFTKDIKGKKFTAQLSEKESFSFKIKKELKNETAVYNMPEKLIAISDIEGEFEAFKQFLIANGVMNAKYQWKYGKGHLVTVGDFFDRGLWVTQTLWLIYHLEQQAEKAGGKVHFILGNHDLMNMNNDFRYVRKKYFQNASLLQDEYLHFYKPNTELGRWLATKNIVEKIGDYVFVHAGISIEIANLGLTVQELNDKARDYYFDNLKARKRKDSLYSILYQFGISPTWYRGWGKQTIDITEAETILERWQVGKFVIGHTLHSEVTYLMNKCVIDLDVAHAKGVIQGLLIENGNEYKVDNQGNKTTIIENASIPEDDD
>NZ_CP022385.1|WP_002678857.1|750031_751165_+|glycosyl-hydrolase-family-5
MNKLVSLVAFLWLIIACEKSGEDNEPSTQTSTTQSTTTQTPTSPPITPPTPPQPPQNNIPYDWDFYLEPSRLNAVKKEPNVELKKLYYQVYGIPCGGWYDGMSPNSEKNNGWLKNFVEGAERAHKTPIVVLYGIPERDCGSFSKGGHPNAASYKAWIDRVSAIIGQRRAIVIIEPDAINYCGHKKGSAKYNERAELLRYAAEKLNKNNPKVMSYIHAGNGPLVTNNPEAIATAIIDGGLKYMRGFALNVSGLGGTAEEQAAAEKFVTYLATKGFKNVHYVIDTGRSGINRPKHQNANAPYNSCNNFNAALGPRSTTKTTGAHADAYLWINGGGGSDGECNMGAPAAGQPYPEYTRALINNAIRVKSIQILELPNNLK
>NZ_CP022385.1|WP_002678663.1|751182_751776_-|M15-family-metallopeptidase
MKYILAFFVTFIATAQQTDFVLLKGLSSDFVFDMKYATPDNFLKQAVYECGECYLRKKTAEALVKANEEFKTLGYRIKLFDCYRPLEVQKKMWKILPGTHYVANPAKGSKHNRGAAVDLTLVDKDGKELDMGTPFDFFGEKAHHTCTTLPKKVLENRKLLKDILNKYNFKSIYSEWWHYEYRPEMQSKVENFQWQCE
>NZ_CP022385.1|WP_002678807.1|751912_752845_-|malate-dehydrogenase
MKVTVVGAGAVGASCAEYIAIKDFASEVVLIDIKEGFAEGKAMDLMQTASLNGFDTRITGVTNDYSKTAGSDVAVITSGIPRKPGMTREELIGTNANIVKSVVEQLVKYSPNVIVIVVSNPMDTMAYLVHKATKLPKNHIIGMGGALDSARFKYRLAEALNSPISDVDGMVIAAHSDSGMLPLTRLASYRGVPVTEFLSADRLNQVAEDTKVGGATLTKLLGTSAWYAPGAAVSALVQAIACDQKKLFPCSVLLEGEYGQKDVCVGVPVIIGRDGVEKIVEVKLNDAEKAKFAESTEAVREVNKALASVL

Crispr_ID: NZ_CP022385_2

• CRISPR_ID: NZ_CP022385_2
• CRISPR_location: 1995454-1995592
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 1 spacers
• Cas_protein_info: DEDDh

Consensus repeat of NZ_CP022385_2

Consensus_repeat	Method
TGAGAAACTCCGAGAAGCTCAGGGCTTTCAGGGAATCTCTAATT	CRISPRCasFinder

spacers of NZ_CP022385_2

>2.1|1995498|51|NZ_CP022385|CRISPRCasFinder
GTTAATTATTAGTTGTTAATTGTTCATTATCTAATTATGACTCTTGAGTTG

CRISPR arrays and Neighbor proteins around NZ_CP022385_2

CRISPR arrays

The CRISPR arrays of NZ_CP022385_2

>merge|NZ_CP022385|2|1995454-1995592|CRISPRCasFinder
TGAGAAACTCCGAGAAGCTCAGGGCTTTCAGGGAATCTCTAATTGTTAATTATTAGTTGTTAATTGTTCATTATCTAATTATGACTCTTGAGTTGTGAGAAACTCTGAGAAGCTCAGGGCTTTCAGGGAATCTCTAATT

>NZ_CP022385|2|1|1995454-1995592|CRISPRCasFinder
TGAGAAACTCCGAGAAGCTCAGGGCTTTCAGGGAATCTCTAATT	GTTAATTATTAGTTGTTAATTGTTCATTATCTAATTATGACTCTTGAGTTG
TGAGAAACTCTGAGAAGCTCAGGGCTTTCAGGGAATCTCTAATT

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
NZ_CP022385.1|WP_002679376.1|1991116_1991566_-|hypothetical-protein	unknown	unknown	unknown
NZ_CP022385.1|WP_002679384.1|2001582_2002119_-|DUF4303-domain-containing-protein	unknown	unknown	gnl|CDD|372922
NZ_CP022385.1|WP_002679409.1|1993410_1993998_-|PorT-family-protein	unknown	unknown	gnl|CDD|372635
NZ_CP022385.1|WP_040362047.1|1982348_1982726_-|glyoxalase	unknown	unknown	gnl|CDD|319947
NZ_CP022385.1|WP_002679253.1|1996652_1997459_-|hypothetical-protein	unknown	unknown	unknown
NZ_CP022385.1|WP_002679482.1|1992310_1993390_-|sensor-histidine-kinase	unknown	unknown	gnl|CDD|225814
NZ_CP022385.1|WP_002679511.1|2002133_2002709_-|DUF4303-domain-containing-protein	unknown	unknown	gnl|CDD|372922
NZ_CP022385.1|WP_002679439.1|1999318_2000893_+|Na+/H+-antiporter	unknown	unknown	gnl|CDD|129911
NZ_CP022385.1|WP_002679378.1|1991621_1992314_-|response-regulator-transcription-factor	unknown	unknown	gnl|CDD|225818
NZ_CP022385.1|WP_002679433.1|2003911_2004961_-|hypothetical-protein	unknown	unknown	unknown
NZ_CP022385.1|WP_002679382.1|1997458_1998517_-|AAA-family-ATPase	unknown	unknown	gnl|CDD|379114
NZ_CP022385.1|WP_002679381.1|2000919_2001225_-|hypothetical-protein	unknown	unknown	unknown
NZ_CP022385.1|WP_002679239.1|1998581_1999220_-|NAD(P)H-binding-protein	unknown	unknown	gnl|CDD|187560
NZ_CP022385.1|WP_002682376.1|1983661_1984156_-|3'-5'-exonuclease	DEDDh	cd06127_DEDDh_CAS-I	gnl|CDD|99834
NZ_CP022385.1|WP_002679260.1|1994838_1995429_-|PorT-family-protein	unknown	unknown	gnl|CDD|372635
NZ_CP022385.1|WP_002679518.1|2004982_2005660_-|tetratricopeptide-repeat-protein	unknown	unknown	gnl|CDD|276809
NZ_CP022385.1|WP_040360811.1|1981945_1982290_+|hypothetical-protein	unknown	unknown	unknown
NZ_CP022385.1|WP_002679282.1|1995620_1996556_-|hypothetical-protein	unknown	unknown	unknown
NZ_CP022385.1|WP_002682374.1|1982802_1983582_-|AraC-family-transcriptional-regulator	unknown	unknown	gnl|CDD|378964
NZ_CP022385.1|WP_002679268.1|1994091_1994727_-|PorT-family-protein	unknown	unknown	gnl|CDD|372635

HMMScan for Signature genes

Protein	Cas_name	Cas_description	E-value	Identity	Coverage
NZ_CP022385.1|WP_002682376.1|1983661_1984156_-|3'-5'-exonuclease	DEDDh	cd06127_DEDDh_CAS-I	1.5e-34	114.2	0.0

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
NZ_CP022385.1|WP_002679384.1|2001582_2002119_-|DUF4303-domain-containing-protein	gnl|CDD|372922	pfam14136, DUF4303, Domain of unknown function (DUF4303). This family of proteins is functionally uncharacterized. This family of proteins is found in bacteria. Proteins in this family are typically between 169 and 192 amino acids in length.	2.16995e-40
NZ_CP022385.1|WP_002679409.1|1993410_1993998_-|PorT-family-protein	gnl|CDD|372635	pfam13568, OMP_b-brl_2, Outer membrane protein beta-barrel domain. This domain is found in a wide range of outer membrane proteins. This domain assumes a membrane bound beta-barrel fold.	4.60149e-08
NZ_CP022385.1|WP_040362047.1|1982348_1982726_-|glyoxalase	gnl|CDD|319947	cd08359, VOC_like, uncharacterized subfamily of vicinal oxygen chelate (VOC) family. The vicinal oxygen chelate (VOC) superfamily is composed of structurally related proteins with paired beta.alpha.beta.beta.beta motifs that provide a metal coordination environment with two or three open or readily accessible coordination sites to promote direct electrophilic participation of the metal ion in catalysis. VOC domain is found in a variety of structurally related metalloproteins, including the bleomycin resistance protein, glyoxalase I, and type I ring-cleaving dioxygenases. A bound metal ion is required for protein activities for the members of this superfamily. A variety of metal ions have been found in the catalytic centers of these proteins including Fe(II), Mn(II), Zn(II), Ni(II) and Mg(II). The protein superfamily contains members with or without domain swapping. The proteins of this family share three conserved metal binding amino acids with the type I extradiol dioxygenases, which shows no domain swapping.	8.12475e-28
NZ_CP022385.1|WP_002679482.1|1992310_1993390_-|sensor-histidine-kinase	gnl|CDD|225814	COG3275, LytS, Putative regulator of cell autolysis [Signal transduction mechanisms].	2.7721e-34
NZ_CP022385.1|WP_002679511.1|2002133_2002709_-|DUF4303-domain-containing-protein	gnl|CDD|372922	pfam14136, DUF4303, Domain of unknown function (DUF4303). This family of proteins is functionally uncharacterized. This family of proteins is found in bacteria. Proteins in this family are typically between 169 and 192 amino acids in length.	2.92534e-05
NZ_CP022385.1|WP_002679439.1|1999318_2000893_+|Na+/H+-antiporter	gnl|CDD|129911	TIGR00831, Putative_Na+/H+_exchanger_Rv2287/MT2345/Mb2309., Na+/H+ antiporter, bacterial form. The Monovalent Cation:Proton Antiporter-1 (CPA1) Family (TC 2.A.36) The CPA1 family is a large family of proteins derived from Gram-positive and Gram-negative bacteria, blue green bacteria, yeast, plants and animals. Transporters from eukaryotes have been functionally characterized, and all of these catalyze Na+:H+ exchange. Their primary physiological functions may be in (1) cytoplasmic pH regulation, extruding the H+ generated during metabolism, and (2) salt tolerance (in plants), due to Na+ uptake into vacuoles. This model is specific for the bacterial members of this family. [Transport and binding proteins, Cations and iron carrying compounds].	5.57702e-84
NZ_CP022385.1|WP_002679378.1|1991621_1992314_-|response-regulator-transcription-factor	gnl|CDD|225818	COG3279, LytT, Response regulator of the LytR/AlgR family [Transcription / Signal transduction mechanisms].	3.04458e-47
NZ_CP022385.1|WP_002679382.1|1997458_1998517_-|AAA-family-ATPase	gnl|CDD|379114	pfam13304, AAA_21, AAA domain, putative AbiEii toxin, Type IV TA system. Several members are annotated as being of the abortive phage resistance system, in which case the family would be acting as the toxin for a type IV toxin-antitoxin resistance system.	1.09332e-17
NZ_CP022385.1|WP_002679518.1|2004982_2005660_-|tetratricopeptide-repeat-protein	gnl|CDD|276809	sd00006, TPR, Tetratricopeptide repeat. The Tetratricopeptide repeat (TPR) typically contains 34 amino acids and is found in a variety of organisms including bacteria, cyanobacteria, yeast, fungi, plants, and humans. It is present in a variety of proteins including those involved in chaperone, cell-cycle, transcription, and protein transport complexes. The number of TPR motifs varies among proteins. Those containing 5-6 tandem repeats generate a right-handed helical structure with an amphipathic channel that is thought to accommodate an alpha-helix of a target protein. It has been proposed that TPR proteins preferentially interact with WD-40 repeat proteins, but in many instances several TPR-proteins seem to aggregate to multi-protein complexes.	2.25353e-05
NZ_CP022385.1|WP_002679239.1|1998581_1999220_-|NAD(P)H-binding-protein	gnl|CDD|187560	cd05250, CC3_like_SDR_a, CC3(TIP30)-like, atypical (a) SDRs. Atypical SDRs in this subgroup include CC3 (also known as TIP30) which is implicated in tumor suppression. Atypical SDRs are distinct from classical SDRs. Members of this subgroup have a glycine rich NAD(P)-binding motif that resembles the extended SDRs, and have an active site triad of the SDRs (YXXXK and upstream Ser), although the upstream Asn of the usual SDR active site is substituted with Asp. For CC3, the Tyr of the triad is displaced compared to the usual SDRs and the protein is monomeric, both these observations suggest that the usual SDR catalytic activity is not present. NADP appears to serve an important role as a ligand, and may be important in the interaction with other macromolecules. Atypical SDRs generally lack the catalytic residues characteristic of the SDRs, and their glycine-rich NAD(P)-binding motif is often different from the forms normally seen in classical or extended SDRs. Atypical SDRs include biliverdin IX beta reductase (BVR-B,aka flavin reductase), NMRa (a negative transcriptional regulator of various fungi), progesterone 5-beta-reductase like proteins, phenylcoumaran benzylic ether and pinoresinol-lariciresinol reductases, phenylpropene synthases, eugenol synthase, triphenylmethane reductase, isoflavone reductases, and others. SDRs are a functionally diverse family of oxidoreductases that have a single domain with a structurally conserved Rossmann fold, an NAD(P)(H)-binding region, and a structurally diverse C-terminal region. Sequence identity between different SDR enzymes is typically in the 15-30% range; they catalyze a wide range of activities including the metabolism of steroids, cofactors, carbohydrates, lipids, aromatic compounds, and amino acids, and act in redox sensing. Classical SDRs have an TGXXX[AG]XG cofactor binding motif and a YXXXK active site motif, with the Tyr residue of the active site motif serving as a critical catalytic residue (Tyr-151, human 15-hydroxyprostaglandin dehydrogenase numbering). In addition to the Tyr and Lys, there is often an upstream Ser and/or an Asn, contributing to the active site; while substrate binding is in the C-terminal region, which determines specificity. The standard reaction mechanism is a 4-pro-S hydride transfer and proton relay involving the conserved Tyr and Lys, a water molecule stabilized by Asn, and nicotinamide. In addition to the Rossmann fold core region typical of all SDRs, extended SDRs have a less conserved C-terminal extension of approximately 100 amino acids, and typically have a TGXXGXXG cofactor binding motif. Complex (multidomain) SDRs such as ketoreductase domains of fatty acid synthase have a GGXGXXG NAD(P)-binding motif and an altered active site motif (YXXXN). Fungal type ketoacyl reductases have a TGXXXGX(1-2)G NAD(P)-binding motif.	8.04659e-57
NZ_CP022385.1|WP_002682376.1|1983661_1984156_-|3'-5'-exonuclease	gnl|CDD|99834	cd06130, DNA_pol_III_epsilon_like, an uncharacterized bacterial subgroup of the DEDDh 3'-5' exonuclease domain family with similarity to the epsilon subunit of DNA polymerase III. This subfamily is composed of uncharacterized bacterial proteins with similarity to the epsilon subunit of DNA polymerase III (Pol III), a multisubunit polymerase which is the main DNA replicating enzyme in bacteria, functioning as the chromosomal replicase. The Pol III holoenzyme is a complex of ten different subunits, three of which (alpha, epsilon, and theta) compose the catalytic core. The Pol III epsilon subunit, encoded by the dnaQ gene, is a DEDDh-type 3'-5' exonuclease which is responsible for the proofreading activity of the polymerase, increasing the fidelity of DNA synthesis. It contains three conserved sequence motifs termed ExoI, ExoII and ExoIII, with a specific Hx(4)D conserved pattern at ExoIII. These motifs are clustered around the active site and contain four conserved acidic residues that serve as ligands for the two metal ions required for catalysis. The epsilon subunit of Pol III also functions as a stabilizer of the holoenzyme complex.	1.00987e-78
NZ_CP022385.1|WP_002679260.1|1994838_1995429_-|PorT-family-protein	gnl|CDD|372635	pfam13568, OMP_b-brl_2, Outer membrane protein beta-barrel domain. This domain is found in a wide range of outer membrane proteins. This domain assumes a membrane bound beta-barrel fold.	1.14055e-11
NZ_CP022385.1|WP_002682374.1|1982802_1983582_-|AraC-family-transcriptional-regulator	gnl|CDD|378964	pfam12833, HTH_18, Helix-turn-helix domain.	2.16283e-07
NZ_CP022385.1|WP_002679268.1|1994091_1994727_-|PorT-family-protein	gnl|CDD|372635	pfam13568, OMP_b-brl_2, Outer membrane protein beta-barrel domain. This domain is found in a wide range of outer membrane proteins. This domain assumes a membrane bound beta-barrel fold.	1.51907e-18

Protein sequences

>NZ_CP022385.1|WP_002679260.1|1994838_1995429_-|PorT-family-protein
MKRIIILTALIAFTATATAQVKFGVRGGYTLSPVSKSIHTDRYENQAHSESASSCGSAYAGLTAEIPASEKLLFDVGLIYINQVTIGEGSTMLHNINLPIAVKYDISGFRPKVGLYASYIPAVTSFEDEHRSLTKNDLKKFDYGATFGLEYNFFDRFFIEANFNLGIANLLKEDPRHSSDYLRTRSILIGVGYRFN
>NZ_CP022385.1|WP_002679268.1|1994091_1994727_-|PorT-family-protein
MKKIITFVALIALTATATAQNESMGFGIRGGGSITGYNGKTEANYTNVKYKAGYYVGIFSDSQLLPFLDLRTELLFSSYGAKWEFTQGNEKYSNTLHTQYINLPILARLKIGNLGILAGVQGGILVNKPSLENTFNGRTVKLDRSDFAKFDFGAVVGAEFNVTDHFLIDIRFNQGLVNALNSSNSSVRNLNISSNNNFLNVSYTLGVGYRF
>NZ_CP022385.1|WP_002679409.1|1993410_1993998_-|PorT-family-protein
MKKIITIIALIALTATATAQVKFGVHAGAGIGLMSKDVHDDRYEGQNKTDKEPFSTSSYGGFFVEIPASEKLLLDLGAMYTNYSTIGKGKTMLHRIDVPLAIKYDISGLRPKVGGYVSYIPGVTSFENKYRSLTRKDIEKFDYGALFGLEYNFNEKLFIETNFYLGLANLLTKDPRHSSDYLRTRSILIGVGYRF
>NZ_CP022385.1|WP_002679482.1|1992310_1993390_-|sensor-histidine-kinase
MNENPYIFLKNNFFLQFLIADRFRIYRHIVACSSLLLFFILGRQWGDYEGMIDYWDWIEKWLGITATFYLNMYVLIPRFFFKGKLATYIILLTLWVLFMLGSTYYLNFVLWKDYMIGKEIKIASEIPVSVAIFSSMVYIVPFIMTSTLIKIFQVWVKDSERMQELERQATESELQALRSQIQPHFLFNMLNSINTLLYIDPEKASYTITKLSDFLRHLLYQTHEEQVFLSSELRFLNDYLSLEAMRRDDFTYELTYSEKNVQGIQIPPNILLILAENAVKHSVDTLSPTYIYMKCEEKEGLLHFSVRNSVPLEPHRKHKCSGIGLPNLNRRLELLYGERFSLSQERLPQEYIVTLTLPL
>NZ_CP022385.1|WP_002679378.1|1991621_1992314_-|response-regulator-transcription-factor
MNCLIIDDEPLARIGMERLIRQYSNLKVVGTFKNAVGVTDFLKKNEVHLLFLDIEMPGINGLELAKILPEQTLVIFTTAYSQYALESYEVDALDYLVKPITPERFKKAIAKAESYHQLLSEQKTDLEATDAQYISIRANRRNYRIAHSDILYIEALKDYVIIHTFTDRYITWINLKNIHSQLPESVFVRVNKSYVVNIQHISSYTHQFVYIGDTEIPIGRTYEVIEKLGN
>NZ_CP022385.1|WP_002679376.1|1991116_1991566_-|hypothetical-protein
MKKIFLFCLALVGFVATAQTYEFKVVTAVESVVPYGVGRSRLISTNDERNYKDFTTTRSEEGDERNKSDRDEIRVKGFDETKLLNFFNIGGIRFQNIAANDALITSKLNAMSEEGWELAFVSSGVESNSGKDDSTGLFITRFVFKRLKK
>NZ_CP022385.1|WP_002682376.1|1983661_1984156_-|3'-5'-exonuclease
MNSFVALDFETANQYRSSVCSIGLVFVENNEIVDTYYQLIKPAPNFYSYFNTQVHGLSQYDTENAPLFPEVWTEILPKIKDLPLVAHNSMFDEGCLREVLAYYALPQHTNPFFCTLRAARKSLPQLVNHKLNTVSAYFGFDLKQHHHALADAEACAHIALELFK
>NZ_CP022385.1|WP_002682374.1|1982802_1983582_-|AraC-family-transcriptional-regulator
MLSLRSYYHPMQSVIPAEEKEVTYTEFLPCEALHPFIYCYWELKSPPRNTPFFYRVVADGCIDLFFDCNAPQMSSLMGYCNTYIEFPITGAFHYVGVRFLPSAFPLIFGQDAFEISAQEIPLSEVVPALATFIAQQLEAPLSLPNIAQHLDNYFLRHLSQRPLQIDNRFFSALLQILQSKGTMHISELDTGISARQLRRLFEYYIGDSPKTFSNIVRFQHILSTKAYHNHSFLDTYYDQAHFIKSFKTFYGDTPSKVLG
>NZ_CP022385.1|WP_040362047.1|1982348_1982726_-|glyoxalase
MKLNAGIITEKLAESKAFYTQVLQFGVTFENDFYLLLHTPNQEAEISFLLPNHPSQQSLFQKPFTQQGVYLTIEVENVDEYYHKLKEQGVPISIDLREEPWGDRHFAIIDPNGIGIDIVTYSAPE
>NZ_CP022385.1|WP_040360811.1|1981945_1982290_+|hypothetical-protein
MKKRFLIAALFTLFLGGIATAQSQEQPSTLAELQAEKERLEAKLAKVLEDKENAKNSLTGKYADVAFAFNRHISKKQKKFAKTVERGAKRQEKQIKKKLKKLNKRIEEISKLEK
>NZ_CP022385.1|WP_002679282.1|1995620_1996556_-|hypothetical-protein
MKEIFTFITLFLGVNSFAQISVKTEYISNSKFYDAVSDSNIGDGSAMIYSVGALVPLSMKAPEEDDLYQRPTLWGVSLSGSFVKMNNHNFPIGKELPSQVMNLGATFLHLRPLKERWSMLMALGAGSYTPENRLSSIHIGENVVANGAFVLVWHWRPNIEIGGGVALNNTFGYPMVFPAFYFKYNGAFSDKFTIEVGSIDGLKVTLGYNYRENLDFKLIANMGGYSAYLRRNGEKEIFSQQTFVVGLQPEFKIGKHVSIPLTVGGSFIRSGRYRERKLSAMFASEAKNEDGSARSSVFLPALYFAAGVTIK
>NZ_CP022385.1|WP_002679253.1|1996652_1997459_-|hypothetical-protein
MKIINLYCEGKRGSHDFDILEKVIEGRLITIKPIGSKYGANAIMEYQEKNGNVRSYFYLLFRDRDFDQPIPEEEKLIIDRKTYYSYRTTIENYLLDVSTFFHFLQEQKNNYKLDTEEAVKALFIKVAKKLKYYQAVRHTLGKLRFNNSFNTTWVTGSGTLPNALDLETCRGEAWQLIENILNKSNQQWTKEIFEATLQEFLTLFEAESFFDELKFLVYYQGKDFAKALTKELPNFSIENYYKYAKKHFDYTKYLDLVELRAIIEKHLV
>NZ_CP022385.1|WP_002679382.1|1997458_1998517_-|AAA-family-ATPase
MKIKEITLRNYKRFVEQKTIFFHKDGEINDLTLIVGNNGTGKTSLLQAIVMMIAPLTRDHFSVEDIDWSGFEYRFIQSGGRMPLKVEATIDFSEEELEKTLLYAKRIDKYGKKIVFPNKNKMINVFFDYEKKKPIVRGKGGGNLFQFFGHQYAKQLTSFEIDKNKLFEGVGNIYWYTEQRTSYSVNNMFEGEVSQLDAIRSFLANAYSFHIAITEGRRTLKAGEFDFYQKISELYGKVFTDRKFVGATPRFDIYEKSTAPDFFLNDGKNDYELSEMSAGERAIFPILMDFARYNINNSIVIIDEIELHLHAPLQQAFIRALPKLGHNNQFILTSHSDYVTTMFEENQIIKLN
>NZ_CP022385.1|WP_002679239.1|1998581_1999220_-|NAD(P)H-binding-protein
MKGLIIGATGATGKDLVTQLLADDTYSEVHCFVRKPMSITHPKLYAHVVNFETPEAWADLLHGDVAFSCLGTTLAVADSKDAQWRVDYDYQYAFAEHCKNNGVPTFVLISAAGATAQSKLFYNRMKGQLEDAIKALDFSCLLIFQPSILIRSNSDRGIENFTVKAFKFLNKLGILKRYRPMPTEILAEKILSAIYNFRKGTFTFALNKIFEL
>NZ_CP022385.1|WP_002679439.1|1999318_2000893_+|Na+/H+-antiporter
MVQYFPYLLLLMVAIMLLIMFANRLKVAYPILLVLGGLAISFIPNVPTITLNPDLVLIIFLPPLLYADVFSLSLKEMWKWRRIIFSFAFIVVFITAAAVAWVANMVFPGFSLALGFLLGGVIGSTDAVSASTIMKFVKVPKRISTIIESESLLNDASSLIVFRFAALVVTTGQLVWTDMALNFLWVVIGGTLIGLGVAWVMRKLHKYLPTDANMDVILTFIVPYIMYIVANEVGASGVLAVVAGGMYMSFHLTSILAASTSNKGWAVWDILGFVLNGLAFMLIGLALPEVLIGIEAEGMSLATATNYGLLITGVVVGVRLLSSYGALLITQVMKHFITVADRRNLGFRLPLVLGWAGMRGVLSLAVALSIPLTLENGEPFPHRSLILYITFIVILATLLVQGLSLPALIRWAKFPDYEDHIPEAEAESLIRKSLAQAALDYMQKHYKEGINDSLLLQNQKDIWQYQLDLSKSNTTTPEERKKYIAILHHQREILRQLNKNPRIDEEQIRNFHRQLNLEEEKWEE
>NZ_CP022385.1|WP_002679381.1|2000919_2001225_-|hypothetical-protein
MSEEEFQFLWQIQSSLSFHYKHAPTFVLCCEQLYLFTPFKIKNIDPKKVEKVGYHYARGGSFLVEIQSPETTKFEVYNSVYPYFASLIEMYNPNADIENYE
>NZ_CP022385.1|WP_002679384.1|2001582_2002119_-|DUF4303-domain-containing-protein
MTPELQPLGGAIFEATQKAFLKLFENGEHYYYCVLLTTGEALPPCIAAWSVEALERFVNENAIPQEEIPYYKYSFADSPYYAFGYEEYFQQVKQLFEQRDSLIDYNNEEQWNEEYNLRLAAMVYAMKKLDETGIFALNQPREQVYINVELMPPDDTDIERALSLNNSEDIKEWLSIFS
>NZ_CP022385.1|WP_002679511.1|2002133_2002709_-|DUF4303-domain-containing-protein
MTQEQKNHIQQLAKEHFRYSFSHIKAKADSKGAHLEAYYLFLVDDFFAVGDAAISREAIKAYDPNATILDAIWSGECEEDYNGSYVPVQENADNWLDNQSHLYKEIYLLVQALEGDDEEEQYNALRKDFEDCLLNALAECDKEGLFGNREENGLLLFAFYIDDYDENDEGSLLYRSTELLNPKKDWDKLKG
>NZ_CP022385.1|WP_002679433.1|2003911_2004961_-|hypothetical-protein
MNEQARKLYDQAQADYPKLKAQIEAQVVRWFWAAGGVGYFSLEPFYFEQNRFSKSKILKDAPEDSDNKYQYGVNANDEIIVERSYTEFKGQCYETFYFRKENQIISYRFEYSEEKECDNVKVFIYKDGLLQYIYSAFEEHYLEETMYYEGNKLIRRKTKGLDYYSNPIDNTLLYTYDMLGKLNSITNETGYVYYQKKDKKISYKALSEKAAERYYALLLPTIKAYPVKEPLYCINLSFDYQNILPTRIGFGTESERQEYQKYGKEAKHYLWNTAEYAHTIDIEPNEEDAALFDLFNQETEMQEKSSAATKLLVACAKRLKEEWASLSIPSTDDFVVVVSDEEESFLKKV
>NZ_CP022385.1|WP_002679518.1|2004982_2005660_-|tetratricopeptide-repeat-protein
MNPIIKQWDTAKSLKRANQYEEALAIYETLCPKVETELSDTFDRAMFFGDYFGLLADVAQYDKAEAMAAKALKYITENEYTTLNYIFYNYGNMYLHQAKWEEAIPWLEKALNRNSDGWINEKRKAYYGTALGGALFHLGRTDEAEEELLKAVEAGKYTVANKDWEPFFYLKEIYKQKGDEKLMAKYEKMYLTRLKKVKEEDLIYPLSEHRANKQALEDWKKLNNP

Crispr_ID: NZ_CP022385_3

• CRISPR_ID: NZ_CP022385_3
• CRISPR_location: 2150212-2150713
• CRISPR_type: TypeII
• Repeat_type: NA
• Spacer_info: 6 spacers
• Cas_protein_info: cas9

Consensus repeat of NZ_CP022385_3

Consensus_repeat	Method
GTTGTTATCAATCACAAAACTACAAAATTTGAAAGCAATTCACAAC	CRISPRCasFinder
GTTGTTATCAATCACAAAACTACAAAATTTGAAAGCAATTCACAAC	PILER-CR

spacers of NZ_CP022385_3

>3.1|2150258|30|NZ_CP022385|CRISPRCasFinder
AAATGCGTTCGCCTGTATCAAGGTCGTTAT
>3.2|2150334|30|NZ_CP022385|CRISPRCasFinder,PILER-CR
TACTAAGGGCGGAGGAGTATCTTTTAGCCG
>3.3|2150410|30|NZ_CP022385|CRISPRCasFinder,PILER-CR
TCGAGGGACACAAGTTGTATATGAACCGTA
>3.4|2150486|30|NZ_CP022385|CRISPRCasFinder,PILER-CR
CCAAAATCCTTTTTTATTCAACAATTCTTC
>3.5|2150562|30|NZ_CP022385|CRISPRCasFinder,PILER-CR
TCATCGCGGTAGTACCACAATTTTTTTACA
>3.6|2150638|30|NZ_CP022385|CRISPRCasFinder,PILER-CR
ACAAATTAAACTATGTTAAGTAGAGTAGAG

CRISPR arrays and Neighbor proteins around NZ_CP022385_3

CRISPR arrays

The CRISPR arrays of NZ_CP022385_3

>merge|NZ_CP022385|3|2150212-2150713|CRISPRCasFinder,PILER-CR
GTTGTTATCAATCACAAAACTACGAAATTTGAAAGCAATTCACAACAAATGCGTTCGCCTGTATCAAGGTCGTTATGTTGTTATCAATCACAAAACTACAAAATTTGAAAGCAATTCACAACTACTAAGGGCGGAGGAGTATCTTTTAGCCGGTTGTTATCAATCACAAAACTACGAAATTTGAAAGCAATTCACAACTCGAGGGACACAAGTTGTATATGAACCGTAGTTGTTATCAATCACAAAACTACAAAATTTGAAAGCAATTCACAACCCAAAATCCTTTTTTATTCAACAATTCTTCGTTGTTATCAATCACAAAACTACAAAATTTGAAAGCAATTCACAACTCATCGCGGTAGTACCACAATTTTTTTACAGTTGTTATCAATCACAAAACTACAAAATTTGAAAGCAATTCACAACACAAATTAAACTATGTTAAGTAGAGTAGAGGTTGTTATCAATCGCAAAACTACAAAATTTGAAAGCAATTCACAAC

>NZ_CP022385|3|2|2150212-2150713|CRISPRCasFinder
GTTGTTATCAATCACAAAACTACGAAATTTGAAAGCAATTCACAAC	AAATGCGTTCGCCTGTATCAAGGTCGTTAT
GTTGTTATCAATCACAAAACTACAAAATTTGAAAGCAATTCACAAC	TACTAAGGGCGGAGGAGTATCTTTTAGCCG
GTTGTTATCAATCACAAAACTACGAAATTTGAAAGCAATTCACAAC	TCGAGGGACACAAGTTGTATATGAACCGTA
GTTGTTATCAATCACAAAACTACAAAATTTGAAAGCAATTCACAAC	CCAAAATCCTTTTTTATTCAACAATTCTTC
GTTGTTATCAATCACAAAACTACAAAATTTGAAAGCAATTCACAAC	TCATCGCGGTAGTACCACAATTTTTTTACA
GTTGTTATCAATCACAAAACTACAAAATTTGAAAGCAATTCACAAC	ACAAATTAAACTATGTTAAGTAGAGTAGAG
GTTGTTATCAATCGCAAAACTACAAAATTTGAAAGCAATTCACAAC

>NZ_CP022385|3|2|2150288-2150713|PILER-CR
GTTGTTATCAATCACAAAACTACAAAATTTGAAAGCAATTCACAAC	TACTAAGGGCGGAGGAGTATCTTTTAGCCG
GTTGTTATCAATCACAAAACTACGAAATTTGAAAGCAATTCACAAC	TCGAGGGACACAAGTTGTATATGAACCGTA
GTTGTTATCAATCACAAAACTACAAAATTTGAAAGCAATTCACAAC	CCAAAATCCTTTTTTATTCAACAATTCTTC
GTTGTTATCAATCACAAAACTACAAAATTTGAAAGCAATTCACAAC	TCATCGCGGTAGTACCACAATTTTTTTACA
GTTGTTATCAATCACAAAACTACAAAATTTGAAAGCAATTCACAAC	ACAAATTAAACTATGTTAAGTAGAGTAGAG
GTTGTTATCAATCGCAAAACTACAAAATTTGAAAGCAATTCACAAC

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
NZ_CP022385.1|WP_040361178.1|2131712_2132669_+|hypothetical-protein	unknown	unknown	unknown
NZ_CP022385.1|WP_002678419.1|2150844_2151282_-|hypothetical-protein	unknown	unknown	gnl|CDD|234827
NZ_CP022385.1|WP_002678556.1|2152089_2152341_+|GlsB/YeaQ/YmgE-family-stress-response-membrane-protein	unknown	unknown	gnl|CDD|225170
NZ_CP022385.1|WP_002679420.1|2132717_2133272_+|TerD-family-protein	unknown	unknown	gnl|CDD|376767
NZ_CP022385.1|WP_002678553.1|2157068_2158109_+|aminotransferase-class-V-fold-PLP-dependent-enzyme	unknown	unknown	gnl|CDD|224919
NZ_CP022385.1|WP_002678372.1|2155510_2156140_+|hypothetical-protein	unknown	unknown	unknown
NZ_CP022385.1|WP_002679408.1|2134010_2134430_+|hypothetical-protein	unknown	unknown	unknown
NZ_CP022385.1|WP_002678487.1|2154231_2154783_+|DUF3127-domain-containing-protein	unknown	unknown	gnl|CDD|378639
NZ_CP022385.1|WP_002678554.1|2152908_2153157_-|hypothetical-protein	unknown	unknown	unknown
NZ_CP022385.1|WP_002679499.1|2133335_2133995_+|tellurium-resistance-protein	unknown	unknown	gnl|CDD|376767
NZ_CP022385.1|WP_002679481.1|2135787_2136831_+|VWA-domain-containing-protein	unknown	unknown	gnl|CDD|238741
NZ_CP022385.1|WP_002678519.1|2145845_2150126_+|type-II-CRISPR-RNA-guided-endonuclease-Cas9	cas9	cd09643_cas9_CAS-II-A:CAS-II-B:CAS-II-C	gnl|CDD|375967
NZ_CP022385.1|WP_002678420.1|2152413_2152791_-|hypothetical-protein	unknown	unknown	unknown
NZ_CP022385.1|WP_002678339.1|2154785_2155424_+|leucyl/phenylalanyl-tRNA--protein-transferase	unknown	unknown	gnl|CDD|234720
NZ_CP022385.1|WP_002679227.1|2134486_2135125_+|VWA-domain-containing-protein	unknown	unknown	gnl|CDD|238741
NZ_CP022385.1|WP_002678524.1|2153146_2153524_-|hypothetical-protein	unknown	unknown	unknown
NZ_CP022385.1|WP_002679255.1|2138208_2139717_+|kinase	unknown	unknown	gnl|CDD|226699
NZ_CP022385.1|WP_002678349.1|2153528_2153855_-|YtxH-domain-containing-protein	unknown	unknown	gnl|CDD|378938
NZ_CP022385.1|WP_002679250.1|2136830_2138207_+|protein-phosphatase-2C-domain-containing-protein	unknown	unknown	gnl|CDD|379325
NZ_CP022385.1|WP_002679473.1|2135127_2135766_+|VWA-domain-containing-protein	unknown	unknown	gnl|CDD|238741

HMMScan for Signature genes

Protein	Cas_name	Cas_description	E-value	Identity	Coverage
NZ_CP022385.1|WP_002678519.1|2145845_2150126_+|type-II-CRISPR-RNA-guided-endonuclease-Cas9	cas9	cd09643_cas9_CAS-II-A:CAS-II-B:CAS-II-C	1.1e-62	207.3	62.2

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
NZ_CP022385.1|WP_002678419.1|2150844_2151282_-|hypothetical-protein	gnl|CDD|234827	PRK00733, hppA, membrane-bound proton-translocating pyrophosphatase; Validated.	0.000680589
NZ_CP022385.1|WP_002678487.1|2154231_2154783_+|DUF3127-domain-containing-protein	gnl|CDD|378639	pfam11325, DUF3127, Domain of unknown function (DUF3127). This bacterial family of proteins has no known function. However, it does show distant similarity to pfam00436, with proteins such as Prevotella buccalis HMPREF0650_0099 being similar to both families. This suggests that this family may have a DNA-binding function.	2.70384e-37
NZ_CP022385.1|WP_002678556.1|2152089_2152341_+|GlsB/YeaQ/YmgE-family-stress-response-membrane-protein	gnl|CDD|225170	COG2261, COG2261, Predicted membrane protein [Function unknown].	1.22072e-05
NZ_CP022385.1|WP_002679420.1|2132717_2133272_+|TerD-family-protein	gnl|CDD|376767	pfam02342, TerD, TerD domain. The TerD domain is found in TerD family proteins that include the paralogous TerD, TerA, TerE, TerF and TerZ proteins It is found in a stress response operon with TerB and TerC. TerD has a maximum of two calcium-binding sites depending on the conservation of aspartates. It has various fusions to nuclease domains, RNA binding domains, ubiquitin related domains, and metal binding domains. The ter gene products lie at the centre of membrane-linked metal recognition complexes with regulatory ramifications encompassing phosphorylation-dependent signal transduction, RNA-dependent regulation, biosynthesis of nucleoside-like metabolites and DNA processing linked to novel pathways.	3.30801e-103
NZ_CP022385.1|WP_002679499.1|2133335_2133995_+|tellurium-resistance-protein	gnl|CDD|376767	pfam02342, TerD, TerD domain. The TerD domain is found in TerD family proteins that include the paralogous TerD, TerA, TerE, TerF and TerZ proteins It is found in a stress response operon with TerB and TerC. TerD has a maximum of two calcium-binding sites depending on the conservation of aspartates. It has various fusions to nuclease domains, RNA binding domains, ubiquitin related domains, and metal binding domains. The ter gene products lie at the centre of membrane-linked metal recognition complexes with regulatory ramifications encompassing phosphorylation-dependent signal transduction, RNA-dependent regulation, biosynthesis of nucleoside-like metabolites and DNA processing linked to novel pathways.	7.7714e-36
NZ_CP022385.1|WP_002679481.1|2135787_2136831_+|VWA-domain-containing-protein	gnl|CDD|238741	cd01464, vWA_subfamily, VWA subfamily: Von Willebrand factor type A (vWA) domain was originally found in the blood coagulation protein von Willebrand factor (vWF). Typically, the vWA domain is made up of approximately 200 amino acid residues folded into a classic a/b para-rossmann type of fold. The vWA domain, since its discovery, has drawn great interest because of its widespread occurrence and its involvement in a wide variety of important cellular functions. These include basal membrane formation, cell migration, cell differentiation, adhesion, haemostasis, signaling, chromosomal stability, malignant transformation and in immune defenses In integrins these domains form heterodimers while in vWF it forms multimers. There are different interaction surfaces of this domain as seen by the various molecules it complexes with. Ligand binding in most cases is mediated by the presence of a metal ion dependent adhesion site termed as the MIDAS motif that is a characteristic feature of most, if not all A domains. Members of this subgroup have no assigned function. This subfamily is typified by the presence of a conserved MIDAS motif.	2.59477e-72
NZ_CP022385.1|WP_002678519.1|2145845_2150126_+|type-II-CRISPR-RNA-guided-endonuclease-Cas9	gnl|CDD|375967	pfam18541, RuvC_III, RuvC endonuclease subdomain 3. Cas9 proteins are abundant across the bacterial kingdom, but vary widely in both sequence and size. All known Cas9 enzymes contain an HNH domain that cleaves the DNA strand complementary to the guide RNA sequence (target strand), and a RuvC nuclease domain required for cleaving the noncomplementary strand (non-target strand), yielding double-strand DNA breaks (DSBs). The crystal structures of type II-A and II-C Cas9 proteins highlight the features in Cas9 enzymes that support their function as RNA-guided endonucleases. Cas9 enzymes adopt a bilobed architecture composed of a nuclease lobe containing juxtaposed RuvC and HNH nuclease domains and a variable alpha-helical lobe likely to be involved in nucleic acid binding. The RuvC domain forms the structural core of the nuclease lobe, a six-stranded beta sheet surrounded by four alpha helices, with all three conserved subdomains (I, II, III) contributing catalytic residues to the active site.	4.62671e-58
NZ_CP022385.1|WP_002678553.1|2157068_2158109_+|aminotransferase-class-V-fold-PLP-dependent-enzyme	gnl|CDD|224919	COG2008, GLY1, Threonine aldolase [Amino acid transport and metabolism].	6.00348e-97
NZ_CP022385.1|WP_002678339.1|2154785_2155424_+|leucyl/phenylalanyl-tRNA--protein-transferase	gnl|CDD|234720	PRK00301, aat, leucyl/phenylalanyl-tRNA--protein transferase; Reviewed.	5.05352e-121
NZ_CP022385.1|WP_002679227.1|2134486_2135125_+|VWA-domain-containing-protein	gnl|CDD|238741	cd01464, vWA_subfamily, VWA subfamily: Von Willebrand factor type A (vWA) domain was originally found in the blood coagulation protein von Willebrand factor (vWF). Typically, the vWA domain is made up of approximately 200 amino acid residues folded into a classic a/b para-rossmann type of fold. The vWA domain, since its discovery, has drawn great interest because of its widespread occurrence and its involvement in a wide variety of important cellular functions. These include basal membrane formation, cell migration, cell differentiation, adhesion, haemostasis, signaling, chromosomal stability, malignant transformation and in immune defenses In integrins these domains form heterodimers while in vWF it forms multimers. There are different interaction surfaces of this domain as seen by the various molecules it complexes with. Ligand binding in most cases is mediated by the presence of a metal ion dependent adhesion site termed as the MIDAS motif that is a characteristic feature of most, if not all A domains. Members of this subgroup have no assigned function. This subfamily is typified by the presence of a conserved MIDAS motif.	1.20976e-77
NZ_CP022385.1|WP_002679255.1|2138208_2139717_+|kinase	gnl|CDD|226699	COG4248, COG4248, Uncharacterized protein with protein kinase and helix-hairpin-helix DNA-binding domains [General function prediction only].	9.95558e-10
NZ_CP022385.1|WP_002678349.1|2153528_2153855_-|YtxH-domain-containing-protein	gnl|CDD|378938	pfam12732, YtxH, YtxH-like protein. This family of proteins is found in bacteria. Proteins in this family are typically between 100 and 143 amino acids in length. The N-terminal region is the most conserved. Proteins is this family are functionally uncharacterized.	1.94121e-13
NZ_CP022385.1|WP_002679250.1|2136830_2138207_+|protein-phosphatase-2C-domain-containing-protein	gnl|CDD|379325	pfam13672, PP2C_2, Protein phosphatase 2C. Protein phosphatase 2C is a Mn++ or Mg++ dependent protein serine/threonine phosphatase.	7.35974e-34
NZ_CP022385.1|WP_002679473.1|2135127_2135766_+|VWA-domain-containing-protein	gnl|CDD|238741	cd01464, vWA_subfamily, VWA subfamily: Von Willebrand factor type A (vWA) domain was originally found in the blood coagulation protein von Willebrand factor (vWF). Typically, the vWA domain is made up of approximately 200 amino acid residues folded into a classic a/b para-rossmann type of fold. The vWA domain, since its discovery, has drawn great interest because of its widespread occurrence and its involvement in a wide variety of important cellular functions. These include basal membrane formation, cell migration, cell differentiation, adhesion, haemostasis, signaling, chromosomal stability, malignant transformation and in immune defenses In integrins these domains form heterodimers while in vWF it forms multimers. There are different interaction surfaces of this domain as seen by the various molecules it complexes with. Ligand binding in most cases is mediated by the presence of a metal ion dependent adhesion site termed as the MIDAS motif that is a characteristic feature of most, if not all A domains. Members of this subgroup have no assigned function. This subfamily is typified by the presence of a conserved MIDAS motif.	3.4843e-58

Protein sequences

>NZ_CP022385.1|WP_002678519.1|2145845_2150126_+|type-II-CRISPR-RNA-guided-endonuclease-Cas9
MKNILGLDLGITSISFAHIVEDENKEKSEIKELGVRIVSLTTDEQSDFEKGKSITTNANRTLKHGARLNLDRYQQRRKYLIDLLQKANLITSSSILAENGKNTTHSTWQLRAKAVTERIEKEEFARVLLAINKKRGYKSSRKAKTEDEGQAIDGMAIAKRLYDENLTPGQLSLQLLQQNKKLLPDFYRSDLQREFDLVWNFQKQFYPDILTDSFYKELQGKGKDATSKAFSKRYHFDTAENKGSKESVRLQAYQWRAEAISKQLSKEEVAYVLTEINNNLNNASGYLGAISDRSKELYFNRQTVGQYLYAQLQENRHNSLKNKVFYRQDYLDEFERIWETQASFHKELTDELKKQIRDVVIFYQRKLKSQKGLISFCEFESKEIEIEKDGKTITKTIGARVVPKSSPLFQEFKIWQILNNVTCKRKGIRKKKSSAKSTELDLFNEPSQTIFSLDMECKQLLFDELNLKGDLKSDKVLKLLGYSPQEWEINYNQLEGNRTQKALYEAYLKIVEMEAHDVKDILQIKSAKDDWSLDESPLSASEIKEKVKDIFQALGICTKILYFDPLLPVKEFEKQDSYQLWHLLYSYESDDSTSGNETLYRILEKKYAFKREHARILANVALQDDYGSLSTKAIRKIYPNIKENQYSKACELAGYKHSKLSLTTEELEARELKNIIPLLKKNALRNPVVEKILNQMINVVNALIEKNSERDAEGKITKYFHFDEIRIELARELKKNAQKRYEMTQNINKAKLEHQKISEILQKKFGIKNPTKSDIIRYRLYQELEHNGYKELYTNAPIARDMLFSKNIEIEHIVPKARVFDDSFSNKTLTFHRINSNKGEYTAFDYITSLNSEEELNQYLTRVENAYKTKSISLTKYKNLLKKASEIGDGFINRDLRDTQYIAKKAKEILFQVTKNVLSTSGSITDRLREDWGLVDVMKELNMPKYQSLGLTEVEERKEGNKVTVIKNWTKRNDHRHHAMDALTVAFTKPSYIQYLNHLNARKDENNKNYSVILAIEEKETIKVPTNNGKNKRVFIEPIPNFRQVAKKHLEEIFISHKAKNKVVTKNVNKPAGTDKQQITLTPRGQLHKETIYGKYQYYVNKEEKVGVKFDERTITKVSNPLYREALLKRLQANDNDPKKAFTGKNALSKNPIYLDETKTKTLPEKVNLTYLEEDFSIRKDISPDNFKDLKSIEKVIDQGVKRILIKRLQDYDNDPKKAFVDLEKNPIWLNKEKGIAIKRVTISGVSNAQPLHIGKDHLGKTTLNKEGNEIPVDYVSTGNNHHVAIYRDKEGNLQEQIVSFFEAVVRTQQGLPIIDKTYKQEEGWQFLFTMKQNEMFVFPNATTGFNPAEIDLLDPKNKKLISPNLFRVQKIATKDYFFRHHLETNVETDNVLKNTTWKREGLSGLKDIVKVRINHLGDIVSIGEY
>NZ_CP022385.1|WP_002679255.1|2138208_2139717_+|kinase
MNTITVKSIIDTSKSYQYVDDGNPKRGGVKDVYFSPDRRYVVAFFRDPLDFNQKERIKRIVSLYLENIKKGNAPDYFLNEIFRWPYDAVEKDGKTGVIVPIYDRKFFFAKGRVADDGLKGEEKKGKWFTTPSFRDPQYPLCLAPSELGDWLSYFQIAINICRGIKKMHQMGLAHSDLSYNNILIDPVTKSANIIDLDGLVVPGMFPPEVIGTADFIAPEVLSTKHLDIKDPKRALPNQKTDLHALAVLIYMYLLRRHPLKGSKVWDLDAEKDELLAMGEKALFIEHPTDATNRVKPDQLRKWDAFWGDPAKLPYTITGPYLTELFNRAFIDGLHNPMLRPIANEWETALLKTVDLIQPCSNPSCTQKWYVFDNTARPKCPFCGTPHRGSLPIADLYYNAGNNVWKPENHRLMVYHNQFLFKWHVSRKLIRNENLTNDDKKPVGYFTFYQNRWVLVNQSLPDMKDVTENKPIPIGTMVELTNEKKIILSNDEGGRLLYITLVN
>NZ_CP022385.1|WP_002679250.1|2136830_2138207_+|protein-phosphatase-2C-domain-containing-protein
MDEIIQTIINAEYPNAQPNPRVAAKVLSILSQSEKISSLAHQLQEEVKEKYQIHYEIESFQDAHLPIANANAKKDYNFSFNLEEFPHIHISRISHLEEFGLTFFPDTRTIEGKPTMAASTELEIHFYCTTDPDLIERVKRVPFIINPDPKDLWQNIPLHKEARFYKTEEATLKTSFLDKKLVVASKRGRSHAHQGGARDDDFYTLPLPNDWQVIAVADGAGSAKFARKGSEIATRFICDSFNDSGFLNTIDQEVVAYFATAEDLTHKSNIVNAIYRKLRELFRHLEDFANAEEIALKDLHTTLIFALAKKYDFGYVLLTFGVGDCPICILNEAQTEVQLLNLLDIGEFGGGTRFITMPEIYSNDAEIPVGKRFGIHRFNDFSKLFLMTDGIYDPKFVVENNLTKIERWNDFLADLNGENDDCCQVDFQNDNQIDQQLLQWLDFWSKGNSDDRTLAIIY
>NZ_CP022385.1|WP_002679481.1|2135787_2136831_+|VWA-domain-containing-protein
MRRLPIYFLLDVSESMVGDPIEHVQDGMATIIKELKADPFALETVWLSIIGFAGKSKVITPLQDIITFYPPKIPIGGGTSLASGLNELMNAIDREVVKTTLERKGDWKPLVFLFTDGIPTDDPAQAIERWNAHYRRKVNLVAISLGENTNYNLLGQLTDQVLQFNNTNAAAYKEFFKWITASIKTTSEQVNNTNTDVIKLAKPDHHLIESIDLTKRHDVPDENFVVIRGKCSNTQKQYLIKFQKSSRSSGIYGMQTRIYMLQGAYKIDENSYKEFSNETNYALKVSSDELMGNPSCPCCGNAYALATCSCGGVHCISGEGENSCPWCGTVANYGMSDEEFDINRTLG
>NZ_CP022385.1|WP_002679473.1|2135127_2135766_+|VWA-domain-containing-protein
MNRRLLAYFLLDTSGSMSGEPIQALNNGFYGLINMLRNDPQAMDSLHLSVITFDREVRNIIPLIDLASFQPIEIKCPESGPTHTGEALEMVCDLVKTELIRGSAQQKGDWKPLLFIFTDGKPSDIQKYREYIPKVKALDFGVIVGCAAGPKAEVSYLQELTPDVVKLDTTDANMLTTFFKWVSSSIEMGGKSQGTGESMTLPPPPRELNLVV
>NZ_CP022385.1|WP_002679227.1|2134486_2135125_+|VWA-domain-containing-protein
MRRLPVYLLLDTSGSMTGEPIEAVKNGVQMMVHSLRQNPQAIETAFVSIITFDSEAKQLIPLTDLASFQMVDLKAAGTTALGAALSLLADKLENEVTKTTLEQKGDWKPIVFIMTDGVPTDDWQAGFQKLKAMKKGLIVGCAAGNNADDKVLKEISDQVVRLSNTDADSIGKFFQWVSASIATTSTKVEETGIDLTKKDQLPPPPSELVIVS
>NZ_CP022385.1|WP_002679408.1|2134010_2134430_+|hypothetical-protein
MNDINNVEGLSTNQIRDLVNQGGKFVIYTYCISLVVVTLKRSSGIYFIKPGKSRVTQGLGWFFLTLILGWWGIPWGPIYTIGCLYNVLSGGKDVTLEVMSFINQNDPEYGASGYGQGLSQNASTYGAGLNNNNSTYNIS
>NZ_CP022385.1|WP_002679499.1|2133335_2133995_+|tellurium-resistance-protein
MAINLQKGQKIDLRKESGEKLQNFCIGVNWGAIETKALFGLMKNKQDVDLDLSCVLIDDNNNVCDHLYSPLYNIQILQQFGLPKGKLDSNDGALHHTGDDLQGDKGGDDGLDNEIITVNLDRVAPNVSKIFFFLNNVGKEDFSQIPYAKIRMFEGTPQRVNSVFASYNVAAEPAYAGKRALILAKLYKRNGEWKFDAIGDPVDDSFLGQTIHRIVQNYL
>NZ_CP022385.1|WP_002679420.1|2132717_2133272_+|TerD-family-protein
MAINLQKGQRESINAPKFTVGLGWDTNSSTTGTDFDLDASVFILGENKKLLSDSHLVFYNNLKSPNEAVVHTGDNRTGAGDGDDEQILVDLSKIEPNAAEICIVVTIHEAAQRGQNFGQVRNSFIRIFDSVTNQEMLKYELEEDFSIETAVEFGRIYKRNGEWKFEAVGMGMKGGLEDYLNKYN
>NZ_CP022385.1|WP_040361178.1|2131712_2132669_+|hypothetical-protein
MGTETKILNDSKRRLQKLKILENFFKQTDLVGIVVRTEIIHSFFEKNTEALDVNKLELFHLQYTDSLIELLTKLKKQKEAAITNIDTEIAAHKDYIAQLARQGKAENRFETDQRYHEAEVSAVFQAAYSTLTGSYKPYNLTAIRSFPNHYAEEYFRDDDDITHLLVVPGTKLYTLEGLNIERLLLSRLASKNFKVRFMCGFNVDFQIFELFRILGSDDEFIWNLQTNGFYLLKDAFKHQLNREKNVSNGHSLVGELERKIRDLEEKRKEIRNSFPEDVKELLAKYRETIDNNNLTEEQLNIEEEKNILTSMLDLNLNK
>NZ_CP022385.1|WP_002678419.1|2150844_2151282_-|hypothetical-protein
MKKSVQIFLELISAIVLYLFINSIAYGDHLVTLPLYLTGVVIMSYLFVRERPQECLQSALRIVLPFTGLMLIAGSLPDAHFSLALIYIVSTGAAFLLGMLIATVSKTYQKIGLSALTFLLVVAVRLSFSAEFQYLIGVHWYTLGS
>NZ_CP022385.1|WP_002678556.1|2152089_2152341_+|GlsB/YeaQ/YmgE-family-stress-response-membrane-protein
MGIIATLIIGAIAGWLGGTIYKGSGLGLIGNIIIGILGSGVGYWLLGNVFHISLGEGWIGAILTGAIGAIVILFLINLVFKKK
>NZ_CP022385.1|WP_002678420.1|2152413_2152791_-|hypothetical-protein
MKKIIALVSVICLSLVAFSCSKNDDPADNDLFVGTYKGSISYLNGQEKKAFDNGSVTVVKTGNDYYFRFSDGIEDLKGVSFKKEGDNTLINVDLQDGLKVIRINASSLYILYTTDGKTWTANAKR
>NZ_CP022385.1|WP_002678554.1|2152908_2153157_-|hypothetical-protein
MNNKTYSSFEEIDNEIRLLRLEKEIDRLSLTQQVSASAESLSPKNLLANTWVSLLFNNKRWINIALEYGMQLLAKRYLNKNR
>NZ_CP022385.1|WP_002678524.1|2153146_2153524_-|hypothetical-protein
MAIYSIKETFVEVPTKANSAFNSQFAYYKLVVFRFIAKSSYGLITFFVYAFASLLILFFLSLAGAYAIGEALGSNGLGFAIVGAFYILLSLVVLLFRKKLIERSLLRKLSEIYFKTDPEEEENEQ
>NZ_CP022385.1|WP_002678349.1|2153528_2153855_-|YtxH-domain-containing-protein
MSKTANAVLGLAVGTAIGVGLGILFAPDEGKNTRKKIKDTLRDKSDDLKEQLDNLTESVREKSLELKGSLEEKVDRLFSKASNKSEDIISLLEKKLASLKEEAAKIKK
>NZ_CP022385.1|WP_002678487.1|2154231_2154783_+|DUF3127-domain-containing-protein
MEIQGRIIKINPTQTVGQNGFQRRDVVIMTEEQYPQYIPFDFVQEKCVLLDNFQEGQTVRISFNIRGREWVNPQGETKYIVNLQGWRIENAEIAPNYPPQGQPYPQGYGQPAYPQQGYAQPQYQQAPPQYQQAAPQYQQQVPPQYPPQMPQQQFQPTPQQAFGTPPVQNAPVAPAEPEDDLPF
>NZ_CP022385.1|WP_002678339.1|2154785_2155424_+|leucyl/phenylalanyl-tRNA--protein-transferase
MIYLINDETPFPSPETASEEGIVAFGGNLNPARLVEAYSQGIFPWYNEGEPVLWWCPDPRFVLFPEKLHISKNMRKLLSKAPYRVTYNRCFTQVMQQCATVPRKDQHGTWIHPELIEAYTTLHQQGIAHSVEVWQDETLVGGLYGLQIGKIFCGESMFSKQPNASQYGFITFLQAHPHIALVDCQIHSEYLESLGAEEIPRATFLKLLTINR
>NZ_CP022385.1|WP_002678372.1|2155510_2156140_+|hypothetical-protein
MNEPLDIILKSVRKVEWRTLKDCLGFIDYNIADILNNLIDDDPHKRNIAYWQLDNQIVIQGGLSESCFYILPFLNELVTITNNRLELLNIIFEIFNGNDRNAQGEHIIIKYNAEIESFEHFIPTSVGREAPLLPSIITYMEANKSVYLKLLNDISSVEELETLLYIILCFKRDPFIKECVYSAKNNEKNSIYNEIFKGFIADIEEVHSL
>NZ_CP022385.1|WP_002678553.1|2157068_2158109_+|aminotransferase-class-V-fold-PLP-dependent-enzyme
MKISFKNDYAEGAHPMILEALMYTNAVQQAGYGNDSYSAAAKELIKNKIENPEAQIFFVSGGTQANMLVIGTLLRTYQSVIAPETGHIADNETGTIEAVGHKIHLAPATNGKITPESITDFATRYTNYPHQVQPRLVYITNATEIGTIYTLAELKAIYKCCQAHNLLLFMDGARLGSALNAEDNDIQWKDLARYTDVFYIGATKNGGLLGEAIVFNKPALADGFEYYLKQQGALLAKGRLLGLQFLTLFENGLYDALALHANKQMKRIKEAFVANGYTLLSNTCTNQLFPILTHTQIAALAEQFDFYQWQKVDETHTAIRLISSWATTDAQVDTLIQAIQLLSVAK

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity
NZ_CP022385_3	3.2|2150334|30|NZ_CP022385|CRISPRCasFinder,PILER-CR	2150334-2150363	30	NZ_CP022385.1	651381-651410	2	0.933
NZ_CP022385_3	3.2|2150334|30|NZ_CP022385|CRISPRCasFinder,PILER-CR	2150334-2150363	30	NZ_CP022385.1	3002943-3002972	2	0.933

1. spacer 3.2|2150334|30|NZ_CP022385|CRISPRCasFinder,PILER-CR matches to position: 651381-651410, mismatch: 2, identity: 0.933

tactaagggcggaggagtatcttttagccg	CRISPR spacer
tactaagggcggaggagcatcttttaaccg	Protospacer
*****************.********.***

2. spacer 3.2|2150334|30|NZ_CP022385|CRISPRCasFinder,PILER-CR matches to position: 3002943-3002972, mismatch: 2, identity: 0.933

tactaagggcggaggagtatcttttagccg	CRISPR spacer
tactaagggcggaggagcatcttttaaccg	Protospacer
*****************.********.***

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity
NZ_CP022385_3	3.4|2150486|30|NZ_CP022385|CRISPRCasFinder,PILER-CR	2150486-2150515	30	CP000314	Clostridium perfringens SM101 plasmid pSM101B, complete sequence	3648-3677	8	0.733
NZ_CP022385_3	3.4|2150486|30|NZ_CP022385|CRISPRCasFinder,PILER-CR	2150486-2150515	30	NZ_MK275625	Clostridium perfringens strain JXJA17 plasmid p7, complete sequence	6323-6352	8	0.733
NZ_CP022385_3	3.4|2150486|30|NZ_CP022385|CRISPRCasFinder,PILER-CR	2150486-2150515	30	NC_014628	Paenibacillus polymyxa SC2 plasmid pSC2, complete sequence	413939-413968	8	0.733
NZ_CP022385_3	3.4|2150486|30|NZ_CP022385|CRISPRCasFinder,PILER-CR	2150486-2150515	30	NC_006872	Clostridium perfringens plasmid pBCNF5603 DNA, complete sequence	24479-24508	8	0.733
NZ_CP022385_3	3.4|2150486|30|NZ_CP022385|CRISPRCasFinder,PILER-CR	2150486-2150515	30	NC_001388	Clostridium perfringens plasmid pIP404, complete sequence	7899-7928	8	0.733
NZ_CP022385_3	3.4|2150486|30|NZ_CP022385|CRISPRCasFinder,PILER-CR	2150486-2150515	30	NZ_CP023411	Clostridium perfringens strain LLY_N11 plasmid pLLY_N11_1, complete sequence	1144-1173	8	0.733
NZ_CP022385_3	3.4|2150486|30|NZ_CP022385|CRISPRCasFinder,PILER-CR	2150486-2150515	30	NZ_CP013044	Clostridium perfringens strain JP55 plasmid pJFP55J, complete sequence	31457-31486	8	0.733
NZ_CP022385_3	3.4|2150486|30|NZ_CP022385|CRISPRCasFinder,PILER-CR	2150486-2150515	30	KT895374	Bacillus phage vB_BpuM-BpSp, complete genome	108746-108775	9	0.7
NZ_CP022385_3	3.5|2150562|30|NZ_CP022385|CRISPRCasFinder,PILER-CR	2150562-2150591	30	NC_011775	Bacillus cereus G9842 plasmid pG9842_209, complete sequence	119020-119049	9	0.7
NZ_CP022385_3	3.5|2150562|30|NZ_CP022385|CRISPRCasFinder,PILER-CR	2150562-2150591	30	CP013001	Bacillus thuringiensis strain XL6 plasmid, complete sequence	281429-281458	9	0.7

1. spacer 3.4|2150486|30|NZ_CP022385|CRISPRCasFinder,PILER-CR matches to CP000314 (Clostridium perfringens SM101 plasmid pSM101B, complete sequence) position: , mismatch: 8, identity: 0.733

ccaaaatccttttttattcaacaattcttc	CRISPR spacer
ctccaatccatttttatttaacaattcaag	Protospacer
*.  ***** ********.********   

2. spacer 3.4|2150486|30|NZ_CP022385|CRISPRCasFinder,PILER-CR matches to NZ_MK275625 (Clostridium perfringens strain JXJA17 plasmid p7, complete sequence) position: , mismatch: 8, identity: 0.733

ccaaaatccttttttattcaacaattcttc	CRISPR spacer
ctccaatccatttttatttaacaattcaag	Protospacer
*.  ***** ********.********   

3. spacer 3.4|2150486|30|NZ_CP022385|CRISPRCasFinder,PILER-CR matches to NC_014628 (Paenibacillus polymyxa SC2 plasmid pSC2, complete sequence) position: , mismatch: 8, identity: 0.733

ccaaaatccttttttattcaacaattcttc	CRISPR spacer
ctcagctccttttttattcaactattcccg	Protospacer
*. *. **************** ****.. 

4. spacer 3.4|2150486|30|NZ_CP022385|CRISPRCasFinder,PILER-CR matches to NC_006872 (Clostridium perfringens plasmid pBCNF5603 DNA, complete sequence) position: , mismatch: 8, identity: 0.733

ccaaaatccttttttattcaacaattcttc	CRISPR spacer
ctccaatccatttttatttaacaattcaag	Protospacer
*.  ***** ********.********   

5. spacer 3.4|2150486|30|NZ_CP022385|CRISPRCasFinder,PILER-CR matches to NC_001388 (Clostridium perfringens plasmid pIP404, complete sequence) position: , mismatch: 8, identity: 0.733

ccaaaatccttttttattcaacaattcttc	CRISPR spacer
ctccaatccatttttatttaacaattcaag	Protospacer
*.  ***** ********.********   

6. spacer 3.4|2150486|30|NZ_CP022385|CRISPRCasFinder,PILER-CR matches to NZ_CP023411 (Clostridium perfringens strain LLY_N11 plasmid pLLY_N11_1, complete sequence) position: , mismatch: 8, identity: 0.733

ccaaaatccttttttattcaacaattcttc	CRISPR spacer
ctccaatccatttttatttaacaattcaag	Protospacer
*.  ***** ********.********   

7. spacer 3.4|2150486|30|NZ_CP022385|CRISPRCasFinder,PILER-CR matches to NZ_CP013044 (Clostridium perfringens strain JP55 plasmid pJFP55J, complete sequence) position: , mismatch: 8, identity: 0.733

ccaaaatccttttttattcaacaattcttc	CRISPR spacer
ctccaatccatttttatttaacaattcaag	Protospacer
*.  ***** ********.********   

8. spacer 3.4|2150486|30|NZ_CP022385|CRISPRCasFinder,PILER-CR matches to KT895374 (Bacillus phage vB_BpuM-BpSp, complete genome) position: , mismatch: 9, identity: 0.7

ccaaaatccttttttattcaacaattcttc	CRISPR spacer
tattaatccttttttattgaacaatatatt	Protospacer
.   ************** ****** . *.

9. spacer 3.5|2150562|30|NZ_CP022385|CRISPRCasFinder,PILER-CR matches to NC_011775 (Bacillus cereus G9842 plasmid pG9842_209, complete sequence) position: , mismatch: 9, identity: 0.7

tcatcgcggtagtaccacaatttttttaca	CRISPR spacer
gtgtaagggttgtaccacaatttttttatg	Protospacer
 ..* . *** *****************..

10. spacer 3.5|2150562|30|NZ_CP022385|CRISPRCasFinder,PILER-CR matches to CP013001 (Bacillus thuringiensis strain XL6 plasmid, complete sequence) position: , mismatch: 9, identity: 0.7

tcatcgcggtagtaccacaatttttttaca	CRISPR spacer
gtgtaagggttgtaccacaatttttttatg	Protospacer
 ..* . *** *****************..

Prophage detection

Region	Region Position	Protein_number	Hit_taxonomy	Key_proteins	Att_site
DBSCAN-SWA_1	621294 : 627182	7	Moraxella_phage(50.0%)	NA	NA
DBSCAN-SWA_2	2184260 : 2192273	8	Synechococcus_phage(28.57%)	NA	NA

Sequences of DBSCAN-SWA_1

Nucleotide sequences of DBSCAN-SWA_1

>NZ_CP022385|621294:627182|DBSCAN-SWA
TTTATATTTTATATTTTTCACTTTTAATGTATTCTTCTACAGAGTCAAGGAAATGAGTTAAATGTTCAATAGTAAAATCTTTATAATTTTTTAATCCTAAAATATTATCTATCTCATTATACGTAATTTCTCTTCCTATTTCAGAAAAAGACTTTTTGCCATGAACTAAGGAATTTCTATATTCTTTTATTTTTTGTAGACATTTACCATGTTTATCCTCATTGTAATAATGTTTTGTAGAAAAGTCAATTCCTAAATCATTTGAGACCTTTCGTATCTTATTAGCATCAAGACTTCCTCCATATTCAATCCTATTAATTATTTCAATAGGTATATTATTTATTATTTCATCTACTATTTGATGAAATCTCTTAAATAGTCTTTTTTCTGTAATAGTTTCATCATATTTATATTTATGTTTAGACCAATATAGTCTAATTTTTTCACTAATATCTGTATATGAAAGATTTTGTTGCTTTATTTCATCAAAAATAGCAACAACAGTATTAAGAATCGTAGATTCAATTAAATTATAAAGTATTAAAAAACCATTAGCTTTTAAAATCTTAAACAATTCATCATTGATAATAAAAGGCTTTTCTTCACCTTTTTTAAATAGAAATACATATTCATACTCTACTTTTTCAAGGAAAGAAAAATAGTTCTCAACTTCACAAACTTTTGAGTCAAAATCATTTCTAAAAATCATTTTTATCCAAAACTTAAATCTTCTTTTTTTATTCTATTAAGTAAACAATCTCTTACAAATTCAATTCTACTTATTAGTTTATTCCTATTATTAGCTGCATCAGATGTTGTATGTATTTTAAATTCTTTAGAATCAATCCAATCCATATACACAGGTTCTAAATTTATATTCTCTCTAAGTGCTAAATTAGTTCCTATTGAAATAGCTTCAAACCTAACTCTAGGAATGGCTTTTGTATTTTCCTCCTTTTTAAATCCATTTGGAAAATATTTTTTAACAAAATTTAGAGTAGCATTCAGTTCATTTTGTTTCTTTATTTTTTCTTCTTCTGTAAAATTATCTTTTTTATTTGAGATATATTCATCTATAAATGTTTTTACAGAATGTGAAAATTCCATATAATTATCTGAATAGGCAAAAAAACGAGAAATTAATTCTTCTTTTTCTCCTCTAAGTTTTTCAGAAGGTTTTGCTGAGGAAAAAACCTCATTAAAAAGAGTACTTTCTGAACAATCTAATATAAAAGAGTAGAAAGAATTTTTCATAAAAGCACCTTTTCGTATCTCTGCTTCTGTTAATTTTTTTCCTGTAGAATTTATTCGGTTGTAAATATCAGCTCTTATACCTTCATTTGCTTTCTCTGAAAAAACAAAGATCCTCAAACCTAAATCCTTAAATTTTCGTTGTCTTGATGGATGTAAATCTTTAAATCTATAATTATTTAAATTTTCTAACAACTTTAAATTTGTAATCTTAAATTTATTATCAACAAACTCCTTTATTGTTGTAAGTCTCTGAACTCCATCTATTAATTCCATATTGCCAGTCTCATCTAAAGTAAAGGCAAATAATGGAGGCATTGGGACTCCTAACATTAAAGATTCTATGAATTTGCATTTTAAATCATTTCCCCAGACAAAATTTCTTTGATAAGAAGGAATAAAGATTATAGAAGATAAGTTACTTTTTTCTAATTCATAATCTCTTCCTTTAAATTTTTCTAAAGCAATTTCTATAGTATATTCTCTAAGATTATAGTTATAATCTTGTTGTAGTTCTTTAATCTGTCTTTCTAACTCTTCTATACTTTCCTTTTTATTTTTCATATTATTTTAGTTTTAAATGCTGTTTTATACTTTTTGCTATCACTTCTCCCAATTTTACTGGAACTGCATTCCCTATTTGTCTTGCTATATTTACAACAGGAAGGCTTCCTTCTTGTTCATTATAAAAAATATAATCTTTAGGAAATGTTTGGAATAAAGATGCTTCTCTTAAAGAAATAGCTCTATCTTGCTCAGGATGTCCAAATCTCCCGTTCCCATATCCTATACAATGAGTTGTCATTGTAGGAGATGGCTCCTCCCATTTCATCCTTCCATATACACTAGGATATGACTTTCCAGACTCTTTTTTATGACAAGCTAATTTAATATCATCATCCCAGTCCTTCCAACTTCCTCCATATGGAGTTTGTTGTATACGTTTCTTATTTAAAGGACTTAACTTTCTTGCATAATGAAGTCTATCCAATTCATTATATTCTCCATCTTTTATAGCAGGTAATTGTCCTATAGCATCTCTTACTGTTACATAATTATCTTTAGAATGAGTTTTATCTATGAGTTTAATATTACCAAATTTGGAAGCTAGTAAAACTAATCTTTTCCTATGCTGAGGAACACCATATTCAGGACAATTTACTATTTCATAATGAATAAAATACCCCATTTCTTCTAAAATTTTTAAAAAATCATTAAAAACAGGTGCTTTCTTAAAATTCAATAATTGTGGAACATTTTCCATAGAAACAATATCTGGATTAACTCCTTTTATCAATCTTGAAAATTCATAAAGAAGTTTCCATTTTTCTGTTTCTCTCTCTTCATCATCTTCTTTTTTATTTTTAAATGAGTATGATGAAAAAGGTTGGCAAGGAGCACATCCTACAAGTATTTTAATTTTTGTATCTTTATATAATTTGTTAATATCTTCCTTTGTAACATCAGAGATATTTTTTGAAATAAATACAGCTCTATTATTTATTTCATAGGCATATTTGCAAGTATTGTCAATATCAAAACCTGCAATAACATTAAAACCCTCTTTAACAAATCCTTGTGATAAACCTCCTATTCCACAAAATAAATCTATGTATAATCCGCAAAACGAAATGTACTAAAAATTAAAACGAAATGTACATTTTTTGAGCGTTTGAGCGGGGGTTGAAAGGGGCAAAGGTAATAAAAAAACACGGATAGGCAGGGTGCTTATCCGTGTTTTTTTGCCTATACATATTATATAGGGTAGTTGGAAGTAAGGACTTCTATACGCTTCTTGCCTGTGCTATTGCTGCTCCCTAAATGCATTGACACTTCTTTTTGATGCCAACCGTGCTGCTGTACGTATTTGGTTAGCTCTTCATTGTGGTAGGAGCTTAGCAGGAATTTGCCTTTGAGGGTGGCAAGTGTGGTTAATAGTTGGTTAAAATGTTCCTGTTCATAACCTCCGTAATGCCCTTGCTTGGCTCCTACATAGGGTGGATCTACATAGTGGAAGGTGTCGGGGGTGTCGTGGCGGGTGAGGACTTCGCTGGCATCGTTGTTGTCTATTTGAACGCCTCGCAGGCGAGCGGAGTAGGTGTCGGTAAAGTTGGTAATTTTGTTGTTGAGGGCTGACACGTTCTTGCTGTTGGTTGTGATACGGCAGTTACCAACTTGGTTAGAGTAACCGCAGTTAGTGGCGTACCAAAATGCCCACGCTTGTTGCACTTCGGTAAAAGCAAAAGGGGCGTGGTAGATTACCAATGCTGACTTGTAGGCTTCACGGCTCACAACTGACTGCTGTACGAGGGTTTGTAGTTCGGCAAAGCGTGTTTGCAGGACTTTATAGAAGGTATAAACATTGGTATTAAAGTCGTTGATGATTTCGGTTTTGACGGGCTGTTTTGCCCAAAAGACTGCACCTCCTCCGAAAAAGGCTTCGGTGTAAATAGTATGTGCAGGGATAAGGGGTAGGATATGTGGCAGCATTGTTTGTTTTCCACCATAGTAGGATATTGGCGTACGTTGCCAAGTATTGGATGTTGATTTCATTGCTTATGTTTCTTAGTATTTGTATATCATTAAATCTTCGTATGAACTTGAGTAATTGACACTTGTGCTAACGGTTACCCGCGTAGTGTTGTTGAATGGACTGGGGAGGTTGTATTCGTTGGCAAGGAAGTCGAAAAGGTCGAGGAGTTGTCCTTTATTGCTTCCGAAGTAGATGTAACGGGGCATTGTATTAAGTGCTTTGACTATGTGTAGGTAGTCCTTTAGCTTCCAATCTTGCGCACCACGGTAGGAACTGATGTCGGTAGAAAGATAGGGTGGGTCGAGGATAAAGACGGTATTTGGGGTATATTCAAACTCGGCGATGAGTTTGCGATAGTCGGTTTGACGACGTTCTACTCCTGCAAGATAACCATCAGCATTGTAGGGTGTTTGAGAAACTTTAGCATAGAAGCCGTCTTTAGCAAGGGCTTCCAAGCTGGTGGCGTACTTGCCGCTGAAAAGGAGATTGGCAGAGAGGGTAATATAATCTAAGGCTTCGGGTGGGTATTGGCGGAGGACTTCCAAAATAGTGGGTTTTACTTCATTGATACGAGTTCCTTTAGGGTGATTTGCTACGATTGGGCGCAATTGGGCGATGATTTCGTTGGTGGTGGGTATGAGTGCCAATCGGTGAGCGAAGTCGTCGTAATCGTTCCATATTACGCGGGCGTTGGGATGAGTGGTTTTGACGGTGTGAGAGAGCAAGCCTGAACCGCCGAATAGGTCGATGTAGGTGGCGTTGGCAGGGAAGTGTTTTAGGGCTTCTTTGAAGTGTTTGACAAATTTTCGCTTTTGCCCTTGAAAAGGTAATGGTGATGTAGTGTGATTTTTCATTTTTTTTAGTGTTAGTGTTCGGTTTATCTTCGGTTAGTGTTCGGTGCGAGCCGCACTGGTAGTGTTTTTTTGGTGTTTTAAAAAATAGTTGTACTTTTGCAGTCCCCAACTTATAGGAAACAAAAAGCACGCTGACGCAGAAGACATATTGTCCTCCGCAGTCAGCGTGCTGATGTTTCTATGAATAAGTTGGGGAACTTTAGTAGAAAAGCGGAGGACATTTTTTATACTGCTATCCTCCTATTTTAGCAGTGCTTTAAAAGCGTTTTAAAAGCTGTTTAAATTCTTATTAAATCTTCCACCGAAACGGCTTAAACTTCCAAATGATAAATACTAACACGGCAATAAGCAAGAGCCAAAGGGTATGCCTTACGGGGCTACTTGTCACTTGTTTGCTTACTTGCTTAACTTGAGTGTATGCGTGTTTTTGTACTTCGGCTTTAGTTCTTGTGTATGAATTATTATAAAGGGTACTATCAGCCTGCTGTAGGCTCTTAGAATGGGTGCTTGTAGCTCGTAGCTTAACCTTTCCGTTGAGTACTCTTATAACTTCATTATCGCCGTCACGAATGCGGGTGTAGATAAGTTCACGTGGTTTGCCTACACTATCGGTGAGGCTTTCGAGTTCGAGTTCAAAGGACTGGTCGGACTGGTGGGACAAGTCTGTTTTGCGACCTTCATAGGCAAAAAGCTGTGAACTATCCTTGTAATGGATAAAGTGCTCTTTCTGGGCGTGACGTTGCTCGGTAGAGGTGACCTTGCGGGTACGGCAACCTACTAAAGCAAGGAACGCTAATAATAATAGGGTTAATTTTCTCATTTGCTAATGTTTTTGTATTCGTCTTTTGCGTTGAAACAAGGGCAAGCTTTAGCTACTCCTGGGAAGTCTCTATGACCTAAGATTTCAGCTTGTGGGTATAAAGCCTTTAACTCTTTGAGAAGCTTTTTAAGGGCTTCTTTTTGGGCATCGGTGCGGGTGTCTTTGGGCTGCAGCGTGTTTTTATCTATCCCCCCAATATAACAGATACCGATGCTGTCCTTATTGTGTCCTTCTACGTGGGCAGGAATTTTATTTACATCTCTGCCCTCTTCTATGGTGCCGTCGAGGCGTACGATGTAGTTGTAGCCGATTTCGTTAAAACCTCTTTGTTTGTGCCATAAATTAATGTCTTTGGCGGTGTGGTCGCGACCTTCGGGGGTTGCCGAGCAGTGTACTACGAGGTAGCGGATGTTGCGTGTGCTTTTTTTCAT

Protein sequences of DBSCAN-SWA_1

>NZ_CP022385|621294:627182|626747_627182_-|WP_002681601.1|DBSCAN-SWA
MKKSTRNIRYLVVHCSATPEGRDHTAKDINLWHKQRGFNEIGYNYIVRLDGTIEEGRDVNKIPAHVEGHNKDSIGICYIGGIDKNTLQPKDTRTDAQKEALKKLLKELKALYPQAEILGHRDFPGVAKACPCFNAKDEYKNISK
>NZ_CP022385|621294:627182|626220_626751_-|WP_002681585.1|DBSCAN-SWA
MRKLTLLLLAFLALVGCRTRKVTSTEQRHAQKEHFIHYKDSSQLFAYEGRKTDLSHQSDQSFELELESLTDSVGKPRELIYTRIRDGDNEVIRVLNGKVKLRATSTHSKSLQQADSTLYNNSYTRTKAEVQKHAYTQVKQVSKQVTSSPVRHTLWLLLIAVLVFIIWKFKPFRWKI
>NZ_CP022385|621294:627182|623109_624177_-|WP_095909094.1|DBSCAN-SWA
MSFCGLYIDLFCGIGGLSQGFVKEGFNVIAGFDIDNTCKYAYEINNRAVFISKNISDVTKEDINKLYKDTKIKILVGCAPCQPFSSYSFKNKKEDDEERETEKWKLLYEFSRLIKGVNPDIVSMENVPQLLNFKKAPVFNDFLKILEEMGYFIHYEIVNCPEYGVPQHRKRLVLLASKFGNIKLIDKTHSKDNYVTVRDAIGQLPAIKDGEYNELDRLHYARKLSPLNKKRIQQTPYGGSWKDWDDDIKLACHKKESGKSYPSVYGRMKWEEPSPTMTTHCIGYGNGRFGHPEQDRAISLREASLFQTFPKDYIFYNEQEGSLPVVNIARQIGNAVPVKLGEVIAKSIKQHLKLK
>NZ_CP022385|621294:627182|625109_625931_-|WP_002681604.1|DBSCAN-SWA
MKNHTTSPLPFQGQKRKFVKHFKEALKHFPANATYIDLFGGSGLLSHTVKTTHPNARVIWNDYDDFAHRLALIPTTNEIIAQLRPIVANHPKGTRINEVKPTILEVLRQYPPEALDYITLSANLLFSGKYATSLEALAKDGFYAKVSQTPYNADGYLAGVERRQTDYRKLIAEFEYTPNTVFILDPPYLSTDISSYRGAQDWKLKDYLHIVKALNTMPRYIYFGSNKGQLLDLFDFLANEYNLPSPFNNTTRVTVSTSVNYSSSYEDLMIYKY
>NZ_CP022385|621294:627182|624299_625097_-|WP_002681612.1|DBSCAN-SWA
MKSTSNTWQRTPISYYGGKQTMLPHILPLIPAHTIYTEAFFGGGAVFWAKQPVKTEIINDFNTNVYTFYKVLQTRFAELQTLVQQSVVSREAYKSALVIYHAPFAFTEVQQAWAFWYATNCGYSNQVGNCRITTNSKNVSALNNKITNFTDTYSARLRGVQIDNNDASEVLTRHDTPDTFHYVDPPYVGAKQGHYGGYEQEHFNQLLTTLATLKGKFLLSSYHNEELTKYVQQHGWHQKEVSMHLGSSNSTGKKRIEVLTSNYPI
>NZ_CP022385|621294:627182|621294_622002_-|WP_002682023.1|DBSCAN-SWA
MIFRNDFDSKVCEVENYFSFLEKVEYEYVFLFKKGEEKPFIINDELFKILKANGFLILYNLIESTILNTVVAIFDEIKQQNLSYTDISEKIRLYWSKHKYKYDETITEKRLFKRFHQIVDEIINNIPIEIINRIEYGGSLDANKIRKVSNDLGIDFSTKHYYNEDKHGKCLQKIKEYRNSLVHGKKSFSEIGREITYNEIDNILGLKNYKDFTIEHLTHFLDSVEEYIKSEKYKI
>NZ_CP022385|621294:627182|622004_623108_-|WP_002682021.1|DBSCAN-SWA
MKNKKESIEELERQIKELQQDYNYNLREYTIEIALEKFKGRDYELEKSNLSSIIFIPSYQRNFVWGNDLKCKFIESLMLGVPMPPLFAFTLDETGNMELIDGVQRLTTIKEFVDNKFKITNLKLLENLNNYRFKDLHPSRQRKFKDLGLRIFVFSEKANEGIRADIYNRINSTGKKLTEAEIRKGAFMKNSFYSFILDCSESTLFNEVFSSAKPSEKLRGEKEELISRFFAYSDNYMEFSHSVKTFIDEYISNKKDNFTEEEKIKKQNELNATLNFVKKYFPNGFKKEENTKAIPRVRFEAISIGTNLALRENINLEPVYMDWIDSKEFKIHTTSDAANNRNKLISRIEFVRDCLLNRIKKEDLSFG

Homologous phage analysis in the prophage region

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_002682023.1|621294_622002_-	hypothetical protein	A0A0R6PKK0	Moraxella_phage	2.7e-10	26.2
WP_002682021.1|622004_623108_-	DUF262 domain-containing protein	A0A0R6PJX3	Moraxella_phage	1.8e-53	37.0
WP_095909094.1|623109_624177_-	DNA (cytosine-5-)-methyltransferase	A0A0R6PG08	Moraxella_phage	8.9e-98	52.1
WP_002681612.1|624299_625097_-	DNA adenine methylase	A0A0K1LM14	Caulobacter_phage	2.4e-15	28.6
WP_002681604.1|625109_625931_-	N6 adenine-specific DNA methyltransferase	H7BVG3	unidentified_phage	3.5e-49	39.5
WP_002681585.1|626220_626751_-	hypothetical protein	NA	NA	NA	NA
WP_002681601.1|626747_627182_-	lysozyme	A0A1B1INV7	uncultured_Mediterranean_phage	4.7e-37	52.2

Sequences of DBSCAN-SWA_2

Nucleotide sequences of DBSCAN-SWA_2

>NZ_CP022385|2184260:2192273|DBSCAN-SWA
ATCATACATTTATTCCTTCAACTAGCGAAATTTCTGCTTTCCAATTAGGTAGTTTTTCTCCCTTATCCCACACTTTCATAACCTCTCTTTTTCTATAAGGTCGTTTACCAAAAGTAATATTTAATTTTTTATTCGTTTGTCGTTCAAAAATCTGAACTATTTCTTTTAATGTATAGATCTCATCTGAAACTACGGCATAATGTTTATGCTTATTTATTTGCTGTGTTTCTAACATCTCAAAAGCTAATATAAAGGCTTTACAAACATCGGAGATGTGTACTAAACTTAACTGTTGTTCACCTGCTGACATATCTAAAGCAGTACCTTCTGTAGCAACTTTTTTAAGTAGATTCATCAGTTTGGGTCGTGTGTCGTTTTCACCATAAGTATCAAACAACACTAAAGTTATTACTTTAAAATCTCCTGCTTGTATGTAATACTCTATTATAGCCTCAAAAGCTTGTTTAGTAGCAGCATATAGACATACAGGGTTATAATCATCATTATTGTAGTATTGCCAAGAGGTGCCTGTATTTATTAAGTTTTTTACATTAGCCTCTTTCATAGCTTCCAAAAGTTGTGTAGAAAATAATATATTGCTATTTATCAATGTATCTACTTCTTCTGTTTTATGCTCTGCAATAAATTTAGAAGCTAAATGAAATACTCCTATGGGCTGTATTTTCTTAAAAAAAGAAACCATTTGCATTATATCTCCTGAATATCTGAAAATATGGGTTTCATCTATTTTTTTTAGAGAGGAAAAATCTGATTCCAATCGGATGATTACAAATACTTCATAGCCCTTTTCTAATAGAGCATTTACTAAATGAGATCCTACGAATCCTGTAGCACCTGTTACTACTACTCGCTTTTTCATTTTAAAAAGGGTTTATAGTTGAAAAATCATCTAAAGTAGGGAAAGATAAATCTCTTGTGGAAAGTATGGGTTTTTCTAAATCCCAATTAAATCCAAAGCTATTGTAATGTATCCCTGCATCACTAGTAGGATTATAAGTTGTACCTACATTATACATCATTATAGTATTGTTTTCTAATGATTTAAAACCGTGTGCTAATCCTTTAGGGATATAGACACTTTTATGGTTTTTATCGCTCAACTCAATAGAGATGTATTGTTTGTAAGTTTTGGATTCTTTTCGTAAATCGAGCACTACATCTATAACACTCCCTGATATTACATATACTAATTTCTGATGGTCATCAGGAGGCAACTGAAAGTGCATCCCTCTTATCACATTTTTGTTAGAAATTGAGTAATAACTTTCATTTATTTGAAAATCAATACCTTTTTGATTTAAAAAATCTGTGTTAAATGTTTTTACAAATAATCCTCTATCATCAAAAGCATTAAAATTTTCTATTATGTAAACCCCCTCAATAGAAGTTGGTATAATTGTTTTCATTTCTTAGAAATTTACTCCAAAATATTCTTCTAATTTTTCTGCGATATAGTCTAATTGTTCTTCACCTAAAGCTGGATAAATACCTACCCAAAAGGTTTGATTCATTGTGATATCGGTATTAGTAAGGTCTCCTACTACACGGTATTCCACTCCTTGGAAATAAGGCTGCTTAGTGAGGTTACCCGCAAACAGCAAACGTGTACCTATCTTGTGCTGGTCTAAGAAGCGGAGCAAATCTACCCGTGCAGTGCCTGCATCTTCTTTAAGGGTGATAGTAAAACCAAACCACGAAGGGTCGCTATTGGGGGTTGCCTCTGGCAGATATAAAAAGTCAGTAAGCGTTTGGAGTCGCTCTTTGAGGTAGGCGAAATTTTTACGGCGTTTTTCTATAAACTCCTCTACGTGGTCTAACTGTGCTAAGCCACAAGCGGCTTGCATGTCGCTGATTTTTAGGTTGTAACCTATATGTGAATACACGTATTTATGGTCGTAACCAAAAGGCAAATCGCCGTGCTGTTGTGAAAATCTACAATTGCAAGTGTTGTCTTTCCCTGGTGCGCAATAGCAATCACGACCCCAGTCGCGGAAAGACTCGGCTATGGTTTTTAGCTCTGCATTATTAGTGAAAACAGCACCTCCCTCGCCCATAGTGATATGGTGAGCTGGATAGAAACTAAGTGTGGCAATATCGCCAAAAGTTCCTACTTTTTGTCCGTTGTAAGTAGCGCCGAGTGCATCGCAACAGTCTTCTACGAGCCAAAGGTTATATTTCTCACAAAGGGCTTTTATTTTCTCTAAGTTGAAAGGATTCCCTAAAGTATGAGCGAGCATAATCGCCTTGGTTTTAGGGGTGATAGCTTGTTCTATAAGGTCGGCGTTTACATTGTGAGTATGTAGGTCTACATCTACAAATACTGGTACAGCCCCGAACTGTATAATAGGGTTCACTGTAGTAGGGAATCCTGCTGCTACGCCTATTACTTCATCACCTTTTTTGATAGCACGATCACCTAATTTAGGCGAAGTGAGGGTATTGAAGGCTACTAAGTTAGCTGAAGAACCTGAATTTACTGTAATGCAGTATTTCACCCCTAGGAAGGCGGCTAACTTCTGTTCAAATGCAGCATTAAAGCGTCCTGTGGTGAGCCAAGCGTCGAGTGAGGCAGCGACCATATTTTGGAGTTCCTGCTCACCGATTACTTTTCCTGAAGGAGGGATTACTGTTTTTCCTGCTTCAAAGGGTTTGGGTTTGTAAGCCTCTTCAGCATATTGGGTTACTAATTTTGCTATTTCTTCTCTAATTGTTTCTAATGACATATTTTCTTTTTTGATTATAAAAGTTTTCAGCTTGCGCTGATGGGGTTAGGATTCCATATATCCTTTGATTTCATATAAGCATCGGGTTTGCATATCTGCTCCTTGTAACCAAGAGCGATGCCAGTGTACGATTTTCTCTAAAGTATCGGCAAGTCGCCATTGAGGATGCCATTTAAGTCGGGTGGCAGCTTTAGAACAGTCGAGTTTCAAGAAATTAGCTTCGTGAGGCTGTTCGCTCTTGTCCCATTCATAACGAGCTCCTTCGCCCCAAAATTGTACCATTTTCTCTAATATCCATTGTACTGATTGGCAATCGTCGTCCTTAGGTCCAAAATTCCAACCTTCGGCAAAGGCATTGCCTTCATTATATAATCGTTCAGCTAATACTAAATAGCCTGAAAGGGGTTCCAAAACGTGTTGCCACGGACGGGTGGATAGTGGGTTGCGGATGATTACAGGTTGTTGTTTTTCGAAAGCGCGCAAGATATCGGGTATTAATCTATCGTCAGCCCAATCGCCACCTCCTATCACATTACCTGCACGGGCTGAGGCTACTGCTGCGGTATCGTTAGTATAAAAAAACGAACGGCGATAGGCAGAGACTACTAACTCAGCACAGCCTTTGCTATTGCTATAAGGGTCGTGTCCGCCCATAGGTTCGTTTTCACGATAGCCCCATTCCCATTCGCGGTTCTCATAACACTTATCGGTAGTGATGATTACCACTGATTTTAGGTTAGAGGCATAACGAGCTGCTTCTAAAACGTTTACCGTACCCATTACATTGGTACTATAAGTGTCTACAGGTTCTTTGTAGGAAAGTCGGACTAAAGGTTGGGCAGCTAAGTGTAACAGCACATCGGGATTGAAAGCACGTATGCTTTGTGATAGCTGCTGGAGGTTGCGAATGTCGCCTATTTCAGTTTGCATATTGTCACCTACTTTAGCTACTTCAAAGAGGGCGGGCTGGGTAGGAGGGGTTAATGAAAATCCTTTTACTTCGGCTCCCATCAGTTGTAGCCATAGTGATAGCCACGAACCTTTGAAGCCCGTGTGCCCTGTAACAAATACGCGTTTGCCTTTCCAAAATTGTGGGTTTACTTTTCCTATTGCCATACTTTCCAAGGAGCTTTATCGTTTTGCCATAATTCTTCTAAGAGCATTTTATCGCGGAGGGTATCCATTGGTTGCCAGAAACCTTCGTGTTTGTAGCTCATCAGCTGTCCGTCGGCAGCTAATCCTTTGAGAGGTTCTTGTTCCCAAATTGTATTATCGTCTTTGATGCGCTCTATCACTTTGGGAGAAAGCACAAAGAAACCTCCGTTTATGAGAGCACCATCGCCTTTGGGTTTTTCTTGAAACTTCATAATCTGATTGTCTATTATATCTAAAGCTCCAAAGCGCCCTGGGGGATAGACAGCTGTGAGGGTGGCTTGCTTGCCGTGTGCTTCATGGAATGCCACTAATTTAGCGATATCTATATCAGCTACCCCATCGCCATAAGTAAAACAGAAAGCTTCATCGTTTTCAATGTATTGCAAAACACGTTTGAGGCGACCTCCCGTCATTGAATGTTCCCCTGTATCAATAAGGGTAACTTTCCAAGGTTCGGCGCGTTTTTGGTGTACGTGCATCTCGTTGTTTGCCATATCGAAAGTGATATCCGATTGGTGCATAAAGTAATTGGCGAAGTACTCTTTGATGATATATCCTTTGTATCCACAACAGATGATGAACTCGTTGATACCATAGTGTGAATAGATTTTGAGGATATGCCACAAGATGGGCATTCCTCCTATTTCTACCATTGGTTTGGGACGTATGGCGGTTTCTTCACTGAGGCGTGTACCGAGACCGCCTGCTAATATTACTGCTTTCATAAAAATTAATCTACTTATTGCTTGAAAATTAATCACTTATTTGCCTGCAACAAATGCATCAGCGAAAAAATTTTCTTCTTTCAATCCTTGTTTAGTGAGAAGTTCCTGAGCGCTTTGTATCATTGCCTCGCTACCACAAGCATACACTTGCGCTGTAGGCCAACTGATAGCCTGCGCTGAAGCTATCTCTTGCACATACCCTTTTGCTCCTTGCCAATCGTCCTCACGAGTCAATACGGGTATATACTCAAAATGACCAAAAGTAAAATCAGGCTTCCAAAATAAGTCTTCTTTTTTTCTTCCTCCCCACAAAAGCCACACTTTTTTATTAACTAATAATTCCGGACTGGCTTGCAGCTGTTCTAAAATGGCTTTTATAGGTGCAATACCAGTACCTGTAGCTAATAATACAATATCGGTGCATACTTCATTATGTCGGTAAAAGAAAGTGCCCAAAGGTCCCTCTACTCTAAGCAAATCATTAGGCTTAGCCTCATTAAACCAATAGTTGCTAAACAGTCCCCTTTCATAATTGCGAATAAAGAGTTTCAGGTGGTTACCTTCATACTGACTATGACTAATAGAATAGCTGCGCTTATGTCCATTTCTGATAATATCTACATATTGCCCCGCTAAAAACTGAAAATCTACCTTAGGAGGCAAACGCAACACTACTTCTACTATATGTGGGGTTAGTGCTGTAATGCTATTGATTTTACAAGGTGTTACTTGTGGTTTAGGCAATGTTACTGCCAAGTCTTCTACGTCTAATACTACTTCTGATCTAGGAACTACATTGCAGGAGAGTACATAGCCTGCTGCTTTTTCTTCTGCTGTAAGGACTTGTTCATCTTGCAAATTCTCTACCTCACCCTCAAGTATTTTTACACGGCAACTGCTGCAACGGGCGTTGAGGCAAGAGTATTCCAAAGCAATACCACTATCCAAAGCGGCTTGTAGCAAAGAGGTGTTCTCTTCACAAGTATAAGACTTATTATTTTTTAAATATATAGTATACTGGTTCAAAATATATTATAGACAATGTTTTTCCTCTTCTATAAACTCTTTTATATGCCTCTTAGCTTTCCAGCCAAGTTCTTCTGTTTTTTTAGTAACCACTTCAGCAGTCATACGGTTGCCTTTGCGTTCAGGCAACATTTGTATCTCTCCACCAAATAACTGTGCAATTTCTAATACTGTATAAGTCTCAGGACTACCAATGCCATAGCCATCACCCTTGCCCTTCTCACCTACGATGAGTAGTCCATCTACAATATCGTCTATGTGGGTGAAATTGCGCTGTTGGGTACCAGGACTCACTACCGTTAGGGGCTCTCCTTTCTTCATTTTTTCAGTAAAAAGAGCTATGAGGGTAGCGTATTTACCGGTGCGTATTTCTCGTTTGCCATAGACATTGTAAAAATATACAATGGCATATTCTAATCCATACCAATTACCATAGTTCTTAACTAATTCGGTATTAGAGGATTTGCTCCACGCATAGGGGCTTTGATCTTTGCCTATACCACCATCACCAAATTTAGTGCTACTGCCTGCATAGACGAGTTTACACCCGCGTTTACGGCAAAACTCTAATACTTTCAGCGTACCTATTTTGTTGAACAATAGAACTTTGTCTATATCGTCGAAACTCTGTTCTACACGAGAATATTCCCCTAAATGGTAAATTAAATCGGGGATGAAATTGATCAACTCAAAAATGTGTTCGGTACTACCTTCTATGTAGGTTACTCCTGGCACGTGGTTGTCTCGGCTTCCGGTAAAGTAATTGTCTAATGAATAGACATCGTTTTGAGGGTCTTTAGCTAGGGCTTCACAAAGGTTTGAGCCTACAAAGCCAGCTCCTCCTGTTACTAATATTTTCATTGTGCAAATGCAGAATTAAAATGAGATTTATCAGTGGGCTCAGAGAGACTCTGAGAAATTTAGATGATTGGGCAATTTTATAAAACAGAATATCAGTTTATTAGAGTTTGACAAAGCTTGAGCTATATGGGAGCTGTGAGAGCTGTGAGAGCTATGGGAATTGTGGGAACTCTCACCTAACTACTAAACTCCTATTTGTATATATTCCCAGCCTTTTTGGGGTAACTCAAAGGCATTGTATTGGTTACGACCGTCAAAGATTACTTTTTCGTTTAGTAATTTGGCTATTTCATCGAAATCGGGGACGCGAAATTCTTTCCATTCGGTAAGTAAAATGAGGGCATCAGCTCCTTTTAGAGCTTCGTATTTGCTCTCTACATAAGTAACCTCTACATCTTTTAGGTAACATACTTTTGCCTCGTGAACTGCTTTGGGATCGTAGGCTTGTACTTTAGCACCGCGTTTCACCAATTCCTTAATGACGTAAATTGCAGGTGCTTCGCGCATATCATCAGTTTCGGGCTTAAATGAGAGCCCCCATACAGCAAAGGTTTTACCACTGAGGTCTTCTCCATATTTGGCAACCACTTTTTGGGCAATCACATATTTTTGTCGGTTGTTCACCTTATCAACGGACTCAATGAGTTCTGCCTTGTAATCAACCTCTTCGGCTAATTTTTTTAATGCCAATACATCTTTTGGGAAGCAGGAACCTCCATACCCACAACCGGGGTAAATAAAACTATAGCCTATACGGCTATCAGATCCTATCCCGATACGCACTTTGTTTACATCAGCCCCTACACGCTCACAGATATTGGCGATCTCGTTCATAAACGATATTTTAGTAGCGAGCATAGTGTTAGCAGCATATTTGGTCATTTCAGCTGAGCGAATGTCCATTATGATCATCCGTTCGTTTTGCATATAAAACGGGCTGTAGAGTTCTTTCATCTTCTTGAAAGCATGTTCGTTATCGGCTCCAATTACCACGCGATCAGGCTTCATAAAGTCTTCAATAGCTTTACCTTCTTTTAAAAACTCGGGATTGGATACTACGTGAAATTTGTAATTCACTCCGCGTTTATCTAAGGCAGTTTGTACAGTAGCACGCACTTTGTCGGCTGTACCTACAGGAACGGTAGATTTATCGACTACTATGAGTTCTCCTTGCATCGTTTCGCCTATTGCTTGGGCTACTGAAAGTACATATTGCAAATCGGCAGACCCATCATCGCCCATAGGCGTTCCCACAGCAATGAAAGCAACCTCGGCATCTTTGATAGCTTCGGCTATATCAGTTGTAAAAAACAAGGTTTGATGAGCTACATTGCTCAGTACCATCTCTTCCAATCCAGGTTCGTAGATGGGGATAATCCCTTGTTTGAGTTTGTTTATCTTTTCAGTATTGACATCTACACAGGTTACTTTATTACCCATTTCGGCAAAGCAAGTGCCTGATACTAAGCCTACATAACCTGTGCCTATAACTGCTATCTTCAT

Protein sequences of DBSCAN-SWA_2

>NZ_CP022385|2184260:2192273|2184260_2185139_-|WP_002678528.1|DBSCAN-SWA
MKKRVVVTGATGFVGSHLVNALLEKGYEVFVIIRLESDFSSLKKIDETHIFRYSGDIMQMVSFFKKIQPIGVFHLASKFIAEHKTEEVDTLINSNILFSTQLLEAMKEANVKNLINTGTSWQYYNNDDYNPVCLYAATKQAFEAIIEYYIQAGDFKVITLVLFDTYGENDTRPKLMNLLKKVATEGTALDMSAGEQQLSLVHISDVCKAFILAFEMLETQQINKHKHYAVVSDEIYTLKEIVQIFERQTNKKLNITFGKRPYRKREVMKVWDKGEKLPNWKAEISLVEGINV
>NZ_CP022385|2184260:2192273|2187048_2188119_-|WP_040360930.1|DBSCAN-SWA
MAIGKVNPQFWKGKRVFVTGHTGFKGSWLSLWLQLMGAEVKGFSLTPPTQPALFEVAKVGDNMQTEIGDIRNLQQLSQSIRAFNPDVLLHLAAQPLVRLSYKEPVDTYSTNVMGTVNVLEAARYASNLKSVVIITTDKCYENREWEWGYRENEPMGGHDPYSNSKGCAELVVSAYRRSFFYTNDTAAVASARAGNVIGGGDWADDRLIPDILRAFEKQQPVIIRNPLSTRPWQHVLEPLSGYLVLAERLYNEGNAFAEGWNFGPKDDDCQSVQWILEKMVQFWGEGARYEWDKSEQPHEANFLKLDCSKAATRLKWHPQWRLADTLEKIVHWHRSWLQGADMQTRCLYEIKGYMES
>NZ_CP022385|2184260:2192273|2188919_2189909_-|WP_002678611.1|DBSCAN-SWA
MNQYTIYLKNNKSYTCEENTSLLQAALDSGIALEYSCLNARCSSCRVKILEGEVENLQDEQVLTAEEKAAGYVLSCNVVPRSEVVLDVEDLAVTLPKPQVTPCKINSITALTPHIVEVVLRLPPKVDFQFLAGQYVDIIRNGHKRSYSISHSQYEGNHLKLFIRNYERGLFSNYWFNEAKPNDLLRVEGPLGTFFYRHNEVCTDIVLLATGTGIAPIKAILEQLQASPELLVNKKVWLLWGGRKKEDLFWKPDFTFGHFEYIPVLTREDDWQGAKGYVQEIASAQAISWPTAQVYACGSEAMIQSAQELLTKQGLKEENFFADAFVAGK
>NZ_CP022385|2184260:2192273|2188109_2188883_-|WP_002678539.1|DBSCAN-SWA
MKAVILAGGLGTRLSEETAIRPKPMVEIGGMPILWHILKIYSHYGINEFIICCGYKGYIIKEYFANYFMHQSDITFDMANNEMHVHQKRAEPWKVTLIDTGEHSMTGGRLKRVLQYIENDEAFCFTYGDGVADIDIAKLVAFHEAHGKQATLTAVYPPGRFGALDIIDNQIMKFQEKPKGDGALINGGFFVLSPKVIERIKDDNTIWEQEPLKGLAADGQLMSYKHEGFWQPMDTLRDKMLLEELWQNDKAPWKVWQ
>NZ_CP022385|2184260:2192273|2189915_2190770_-|WP_002678373.1|DBSCAN-SWA
MKILVTGGAGFVGSNLCEALAKDPQNDVYSLDNYFTGSRDNHVPGVTYIEGSTEHIFELINFIPDLIYHLGEYSRVEQSFDDIDKVLLFNKIGTLKVLEFCRKRGCKLVYAGSSTKFGDGGIGKDQSPYAWSKSSNTELVKNYGNWYGLEYAIVYFYNVYGKREIRTGKYATLIALFTEKMKKGEPLTVVSPGTQQRNFTHIDDIVDGLLIVGEKGKGDGYGIGSPETYTVLEIAQLFGGEIQMLPERKGNRMTAEVVTKKTEELGWKAKRHIKEFIEEEKHCL
>NZ_CP022385|2184260:2192273|2185140_2185686_-|WP_002678310.1|DBSCAN-SWA
MKTIIPTSIEGVYIIENFNAFDDRGLFVKTFNTDFLNQKGIDFQINESYYSISNKNVIRGMHFQLPPDDHQKLVYVISGSVIDVVLDLRKESKTYKQYISIELSDKNHKSVYIPKGLAHGFKSLENNTIMMYNVGTTYNPTSDAGIHYNSFGFNWDLEKPILSTRDLSFPTLDDFSTINPF
>NZ_CP022385|2184260:2192273|2190953_2192273_-|WP_002678394.1|DBSCAN-SWA
MKIAVIGTGYVGLVSGTCFAEMGNKVTCVDVNTEKINKLKQGIIPIYEPGLEEMVLSNVAHQTLFFTTDIAEAIKDAEVAFIAVGTPMGDDGSADLQYVLSVAQAIGETMQGELIVVDKSTVPVGTADKVRATVQTALDKRGVNYKFHVVSNPEFLKEGKAIEDFMKPDRVVIGADNEHAFKKMKELYSPFYMQNERMIIMDIRSAEMTKYAANTMLATKISFMNEIANICERVGADVNKVRIGIGSDSRIGYSFIYPGCGYGGSCFPKDVLALKKLAEEVDYKAELIESVDKVNNRQKYVIAQKVVAKYGEDLSGKTFAVWGLSFKPETDDMREAPAIYVIKELVKRGAKVQAYDPKAVHEAKVCYLKDVEVTYVESKYEALKGADALILLTEWKEFRVPDFDEIAKLLNEKVIFDGRNQYNAFELPQKGWEYIQIGV
>NZ_CP022385|2184260:2192273|2185689_2187003_-|WP_002678521.1|DBSCAN-SWA
MSLETIREEIAKLVTQYAEEAYKPKPFEAGKTVIPPSGKVIGEQELQNMVAASLDAWLTTGRFNAAFEQKLAAFLGVKYCITVNSGSSANLVAFNTLTSPKLGDRAIKKGDEVIGVAAGFPTTVNPIIQFGAVPVFVDVDLHTHNVNADLIEQAITPKTKAIMLAHTLGNPFNLEKIKALCEKYNLWLVEDCCDALGATYNGQKVGTFGDIATLSFYPAHHITMGEGGAVFTNNAELKTIAESFRDWGRDCYCAPGKDNTCNCRFSQQHGDLPFGYDHKYVYSHIGYNLKISDMQAACGLAQLDHVEEFIEKRRKNFAYLKERLQTLTDFLYLPEATPNSDPSWFGFTITLKEDAGTARVDLLRFLDQHKIGTRLLFAGNLTKQPYFQGVEYRVVGDLTNTDITMNQTFWVGIYPALGEEQLDYIAEKLEEYFGVNF

Homologous phage analysis in the prophage region

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_002678528.1|2184260_2185139_-	NAD(P)-dependent oxidoreductase	M4QPK0	Synechococcus_phage	2.3e-14	27.1
WP_002678310.1|2185140_2185686_-	dTDP-4-keto-6-deoxy-D-glucose epimerase	H9NC63	Sphingomonas_phage	5.5e-19	36.0
WP_002678521.1|2185689_2187003_-	lipopolysaccharide biosynthesis protein RfbH	A0A0P0YLZ6	Yellowstone_lake_phycodnavirus	3.8e-50	33.0
WP_040360930.1|2187048_2188119_-	CDP-glucose 4,6-dehydratase	K7QJG5	Escherichia_phage	1.7e-11	23.8
WP_002678539.1|2188109_2188883_-	glucose-1-phosphate cytidylyltransferase	NA	NA	NA	NA
WP_002678611.1|2188919_2189909_-	2Fe-2S iron-sulfur cluster binding domain-containing protein	A0A1D8KP97	Synechococcus_phage	8.0e-08	40.3
WP_002678373.1|2189915_2190770_-	NAD-dependent epimerase/dehydratase family protein	A0A0P0YMS6	Yellowstone_lake_phycodnavirus	1.3e-22	28.4
WP_002678394.1|2190953_2192273_-	UDP-glucose/GDP-mannose dehydrogenase family protein	A0A127AXI2	Bacillus_phage	3.3e-78	38.2