CRISPRimmunity

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Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Self targeting spacer number	Target MGE spacer number	Prophage number
NZ_CP017203	Borreliella burgdorferi strain B331 plasmid B331_cp32_1, complete sequence	0 crisprs	NA	0	0	0
NZ_CP017206	Borreliella burgdorferi strain B331 plasmid B331_cp32_4, complete sequence	0 crisprs	NA	0	0	0
NZ_CP017211	Borreliella burgdorferi strain B331 plasmid B331_lp17, complete sequence	0 crisprs	NA	0	0	0
NZ_CP017215	Borreliella burgdorferi strain B331 plasmid B331_lp28_6, complete sequence	0 crisprs	NA	0	0	0
NZ_CP017209	Borreliella burgdorferi strain B331 plasmid B331_cp32_7, complete sequence	0 crisprs	NA	0	0	0
NZ_CP017216	Borreliella burgdorferi strain B331 plasmid B331_lp36, complete sequence	0 crisprs	NA	0	0	0
NZ_CP017202	Borreliella burgdorferi strain B331 plasmid B331_cp26, complete sequence	0 crisprs	NA	0	0	0
NZ_CP017207	Borreliella burgdorferi strain B331 plasmid B331_cp32_5, complete sequence	0 crisprs	NA	0	0	0
NZ_CP017204	Borreliella burgdorferi strain B331 plasmid B331_cp32_10, complete sequence	1 crisprs	NA	1	2	0
NZ_CP017210	Borreliella burgdorferi strain B331 plasmid B331_cp9, complete sequence	0 crisprs	NA	0	0	0
NZ_CP017214	Borreliella burgdorferi strain B331 plasmid B331_lp28_5, complete sequence	0 crisprs	NA	0	0	0
NZ_CP017212	Borreliella burgdorferi strain B331 plasmid B331_lp25, complete sequence	0 crisprs	NA	0	0	0
NZ_CP017201	Borreliella burgdorferi strain B331 chromosome, complete genome	0 crisprs	NA	0	0	1
NZ_CP017208	Borreliella burgdorferi strain B331 plasmid B331_cp32_6, complete sequence	0 crisprs	NA	0	0	0
NZ_CP017217	Borreliella burgdorferi strain B331 plasmid B331_lp38, complete sequence	0 crisprs	NA	0	0	0
NZ_CP017213	Borreliella burgdorferi strain B331 plasmid B331_lp28_3, complete sequence	0 crisprs	NA	0	0	0
NZ_CP017218	Borreliella burgdorferi strain B331 plasmid B331_lp54, complete sequence	0 crisprs	NA	0	0	0
NZ_CP017205	Borreliella burgdorferi strain B331 plasmid B331_cp32_11, complete sequence	0 crisprs	NA	0	0	0
NZ_CP017219	Borreliella burgdorferi strain B331 plasmid B331_lp56, complete sequence	0 crisprs	NA	0	0	0

Results visualization

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CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region	Region Position	Protein_number	Hit_taxonomy	Key_proteins	Att_site	Prophage annotation

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

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CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

447770 : 457190

Streptococcus_phage(28.57%)

The bacterium proteins that are colored denote the protein is present at specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin' and 'tRNA')

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_002656220.1\|447770_448487_-	16S rRNA (cytidine(1402)-2'-O)-methyltransferase	M1PLC5	Streptococcus_phage	1.1e-35	40.8
WP_002657899.1\|448656_448974_+	hypothetical protein	NA	NA	NA	NA
WP_002557021.1\|449003_449393_-	DUF1893 domain-containing protein	NA	NA	NA	NA
WP_002656815.1\|449506_450112_+	YigZ family protein	A0A1X9I5T8	Streptococcus_phage	9.8e-17	30.5
WP_002658820.1\|450220_450973_+	ParA family protein	H7BUL8	unidentified_phage	1.1e-17	28.2
WP_002662385.1\|450975_451689_+	hypothetical protein	A0A1P8CWU6	Bacillus_phage	1.2e-24	38.7
WP_002662380.1\|451940_452723_-	ParB/RepB/Spo0J family partition protein	I3NLC2	Bifidobacterium_phage	3.8e-13	30.1
WP_002658988.1\|452841_455274_-	DNA topoisomerase (ATP-hydrolyzing) subunit A	G3M9Z5	Bacillus_virus	1.0e-104	32.9
WP_002662387.1\|455285_457190_-	DNA topoisomerase (ATP-hydrolyzing) subunit B	G3M9Z3	Bacillus_virus	3.4e-132	43.7

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

3. NZ_CP017204

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CRISPR-Cas detection and classification

Crispr_ID: NZ_CP017204_1

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

NZ_CP017204_1

22450-22645

Orphan

Consensus_repeat	Method
ACACTAAAATAGATAATGTAGAAAAGAATTTAAAC	PILER-CR

2 spacers

The CRISPR arrays of NZ_CP017204_1

>merge|NZ_CP017204|1|22450-22645|PILER-CR
TAATGTAGAAAAGAATTTACAAAAGGATATATCTAATTTAGACATTAAGATTGATACTGTAGAAAAGAATTTAAACACTAAAATAGACAATGTTGAAAAGAATTTAAACACTAAAATAGACAATGTTGAAAAGAATTTAAATCTAAAAATAGATAATTTAGACTCTAAAATAGATGCTGTAGAAAAGAATTTAAAC

>NZ_CP017204|1|1|22450-22645|PILER-CR
TAATGTAGAAAAGAATTTACAAAAGGATATATCTAATTT	AGACATTAAGATTGATACTGTAGAAAAGAATTTAAACACTAAAATAGACAATG
TTGAAAAGAATTTAAACACTAAAATAGACAATGTTGAAA	AGAATTTAAATCTAAAAATAGATAATTTAGACTCTAAAATAGATGCTGTAG
AAAAGAATTTAAAC

Protein	Signature genes	Signature genes Name	Protein_function
NZ_CP017204.1\|WP_012593001.1\|21751_22294_+\|chromosome-replication/partitioning-protein	unknown	unknown	gnl\|CDD\|376591
NZ_CP017204.1\|WP_032492911.1\|28276_28843_+\|DUF603-domain-containing-protein	unknown	unknown	gnl\|CDD\|282496
NZ_CP017204.1\|WP_002658486.1\|15712_16048_+\|hypothetical-protein	unknown	unknown	gnl\|CDD\|368373
NZ_CP017204.1\|WP_096890817.1\|27552_28095_+\|DUF643-domain-containing-protein	unknown	unknown	gnl\|CDD\|282691
NZ_CP017204.1\|WP_096890811.1\|20428_20995_+\|DUF226-domain-containing-protein	unknown	unknown	gnl\|CDD\|308504
NZ_CP017204.1\|WP_096890807.1\|16855_17281_+\|Mlp-family-lipoprotein	unknown	unknown	gnl\|CDD\|308750
NZ_CP017204.1\|WP_096890816.1\|26336_27359_+\|laminin-binding-protein-ErpX	unknown	unknown	gnl\|CDD\|311003
NZ_CP017204.1\|WP_012621296.1\|24680_25106_+\|DUF261-domain-containing-protein	unknown	unknown	gnl\|CDD\|281224
NZ_CP017204.1\|WP_096890814.1\|23302_24628_+\|DUF244-domain-containing-protein	unknown	unknown	gnl\|CDD\|281152
NZ_CP017204.1\|WP_086440117.1\|16040_16394_+\|hypothetical-protein	unknown	unknown	gnl\|CDD\|308571
NZ_CP017204.1\|WP_096890818.1\|28839_30192_+\|PBSX-family-phage-terminase-large-subunit	unknown	unknown	gnl\|CDD\|273684
NZ_CP017204.1\|WP_010883868.1\|15375_15723_+\|holin,-BlyB-family	unknown	unknown	gnl\|CDD\|368373
NZ_CP017204.1\|WP_096890812.1\|20970_21711_+\|ParA-family-protein	unknown	unknown	gnl\|CDD\|224113
NZ_CP017204.1\|WP_096890808.1\|17302_17917_+\|Mlp-family-lipoprotein	unknown	unknown	gnl\|CDD\|308750
NZ_CP017204.1\|WP_096890809.1\|17993_18941_-\|ERF-family-protein	unknown	unknown	gnl\|CDD\|377346
NZ_CP017204.1\|WP_096890810.1\|19318_20419_+\|hypothetical-protein	unknown	unknown	gnl\|CDD\|367076
NZ_CP017204.1\|WP_096890815.1\|25111_25906_-\|site-specific-integrase	unknown	unknown	gnl\|CDD\|271175

Protein	Function_ID	Function_description	E-value
NZ_CP017204.1\|WP_012593001.1\|21751_22294_+\|chromosome-replication/partitioning-protein	gnl\|CDD\|376591	pfam01672, Plasmid_parti, Putative plasmid partition protein. This family consists of conserved hypothetical proteins from Borrelia burgdorferi the lyme disease spirochaete, some of which are putative plasmid partition proteins.	3.74758e-34
NZ_CP017204.1\|WP_032492911.1\|28276_28843_+\|DUF603-domain-containing-protein	gnl\|CDD\|282496	pfam04645, DUF603, Protein of unknown function, DUF603. This family includes several uncharacterized proteins from Borrelia species.	2.77658e-105
NZ_CP017204.1\|WP_002658486.1\|15712_16048_+\|hypothetical-protein	gnl\|CDD\|368373	pfam05289, BLYB, Borrelia hemolysin accessory protein. This family consists of several borrelia hemolysin accessory proteins (BLYB). BLYB was thought to be an accessory protein, which was proposed to comprise a hemolysis system but it is now thought that BlyA and BlyB function instead as a prophage-encoded holin or holin-like system.	1.27368e-28
NZ_CP017204.1\|WP_096890817.1\|27552_28095_+\|DUF643-domain-containing-protein	gnl\|CDD\|282691	pfam04867, DUF643, Protein of unknown function (DUF643). Protein of unknown function found in Borrelia burgdorferi, the Lyme disease spirochete.	1.26399e-35
NZ_CP017204.1\|WP_096890811.1\|20428_20995_+\|DUF226-domain-containing-protein	gnl\|CDD\|308504	pfam02890, DUF226, Borrelia family of unknown function DUF226. This family of proteins are found in Borrelia. The proteins are about 190 amino acids long and have no known function.	2.77332e-59
NZ_CP017204.1\|WP_096890807.1\|16855_17281_+\|Mlp-family-lipoprotein	gnl\|CDD\|308750	pfam03304, Mlp, Mlp lipoprotein family. The Mlp (for Multicopy Lipoprotein) family of lipoproteins is found in Borrelia species. This family were previously known as 2.9 lipoprotein genes. These surface expressed genes may represent new candidate vaccinogens for Lyme disease. Members of this family generally are downstream of four ORFs called A,B,C and D that are involved in hemolytic activity.	5.01302e-46
NZ_CP017204.1\|WP_096890816.1\|26336_27359_+\|laminin-binding-protein-ErpX	gnl\|CDD\|311003	pfam06780, Erp_C, Erp protein C-terminus. This family represents the C-terminus of bacterial Erp proteins that seem to be specific to Borrelia burgdorferi (a causative agent of Lyme disease). Borrelia Erp proteins are particularly heterogeneous, which might enable them to interact with a wide variety of host components.	4.08268e-59
NZ_CP017204.1\|WP_012621296.1\|24680_25106_+\|DUF261-domain-containing-protein	gnl\|CDD\|281224	pfam03196, DUF261, Protein of unknown function, DUF261.	4.87531e-70
NZ_CP017204.1\|WP_096890814.1\|23302_24628_+\|DUF244-domain-containing-protein	gnl\|CDD\|281152	pfam03112, DUF244, Uncharacterized protein family (ORF7) DUF. Several members of this family are Borrelia burgdorferi plasmid proteins of uncharacterized function.	4.31889e-99
NZ_CP017204.1\|WP_086440117.1\|16040_16394_+\|hypothetical-protein	gnl\|CDD\|308571	pfam02999, Borrelia_orfD, Borrelia orf-D family. Borrelia burgdorferi supercoiled plasmids encode multicopy tandem open reading frames called Orf-A, Orf-B, Orf-C and Orf-D. This family corresponds to Orf-D. The putative product of this gene has no known function.	7.07902e-39
NZ_CP017204.1\|WP_096890818.1\|28839_30192_+\|PBSX-family-phage-terminase-large-subunit	gnl\|CDD\|273684	TIGR01547, Uncharacterized_protein_YqaT, phage terminase, large subunit, PBSX family. This model detects members of a highly divergent family of the large subunit of phage terminase. All members are encoded by phage genomes or within prophage regions of bacterial genomes. This is a distinct family from pfam03354. [Mobile and extrachromosomal element functions, Prophage functions].	1.87669e-44
NZ_CP017204.1\|WP_010883868.1\|15375_15723_+\|holin,-BlyB-family	gnl\|CDD\|368373	pfam05289, BLYB, Borrelia hemolysin accessory protein. This family consists of several borrelia hemolysin accessory proteins (BLYB). BLYB was thought to be an accessory protein, which was proposed to comprise a hemolysis system but it is now thought that BlyA and BlyB function instead as a prophage-encoded holin or holin-like system.	1.06895e-39
NZ_CP017204.1\|WP_096890812.1\|20970_21711_+\|ParA-family-protein	gnl\|CDD\|224113	COG1192, Soj, ATPases involved in chromosome partitioning [Cell division and chromosome partitioning].	6.78185e-54
NZ_CP017204.1\|WP_096890808.1\|17302_17917_+\|Mlp-family-lipoprotein	gnl\|CDD\|308750	pfam03304, Mlp, Mlp lipoprotein family. The Mlp (for Multicopy Lipoprotein) family of lipoproteins is found in Borrelia species. This family were previously known as 2.9 lipoprotein genes. These surface expressed genes may represent new candidate vaccinogens for Lyme disease. Members of this family generally are downstream of four ORFs called A,B,C and D that are involved in hemolytic activity.	3.81329e-34
NZ_CP017204.1\|WP_096890809.1\|17993_18941_-\|ERF-family-protein	gnl\|CDD\|377346	pfam04404, ERF, ERF superfamily. The DNA single-strand annealing proteins (SSAPs), such as RecT, Red-beta, ERF and Rad52, function in RecA-dependent and RecA-independent DNA recombination pathways. This family includes proteins related to ERF.	1.01589e-40
NZ_CP017204.1\|WP_096890810.1\|19318_20419_+\|hypothetical-protein	gnl\|CDD\|367076	pfam02414, Borrelia_orfA, Borrelia ORF-A. This protein is encoded by an open reading frame in plasmid borne DNA repeats of Borrelia species. This protein is known as ORF-A. The function of this putative protein is unknown.	3.76166e-63
NZ_CP017204.1\|WP_096890815.1\|25111_25906_-\|site-specific-integrase	gnl\|CDD\|271175	cd00397, DNA_BRE_C, DNA breaking-rejoining enzymes, C-terminal catalytic domain. The DNA breaking-rejoining enzyme superfamily includes type IB topoisomerases and tyrosine based site-specific recombinases (integrases) that share the same fold in their catalytic domain containing conserved active site residues. The best-studied members of this diverse superfamily include Human topoisomerase I, the bacteriophage lambda integrase, the bacteriophage P1 Cre recombinase, the yeast Flp recombinase, and the bacterial XerD/C recombinases. Their overall reaction mechanism is essentially identical and involves cleavage of a single strand of a DNA duplex by nucleophilic attack of a conserved tyrosine to give a 3' phosphotyrosyl protein-DNA adduct. In the second rejoining step, a terminal 5' hydroxyl attacks the covalent adduct to release the enzyme and generate duplex DNA. The enzymes differ in that topoisomerases cleave and then rejoin the same 5' and 3' termini, whereas a site-specific recombinase transfers a 5' hydroxyl generated by recombinase cleavage to a new 3' phosphate partner located in a different duplex region. Many DNA breaking-rejoining enzymes also have N-terminal domains, which show little sequence or structure similarity.	2.40084e-07

>NZ_CP017204.1|WP_012593001.1|21751_22294_+|chromosome-replication/partitioning-protein
MDIKVNKRDLSDNENYIITDSVLNHYNSLKEKLKINSKKEIYCKLETLKILKEIKDNHYYRFDGYKSFEAFSKDYRLARAQVYNYLKIANAIEDGIIQEEFLIKNGILETLIVLRNKESKTIKKSKQNPIKPLRFQLKRQESYDFYKSNAKFTGFMLDKLFSDGKEIIKKLMKEYKELKG
>NZ_CP017204.1|WP_096890812.1|20970_21711_+|ParA-family-protein
MDNKKPKIITIASIKGGVGKSTSAIILATLLSKNNKVLLIDMDTQASITSYFYEKIEKLGINFTKFNIYEILKENVDIDSTIINVDNNLDLIPSYLTLHNFSEDKIEHKDFLLKTSLGTLYYKYDYIVIDTNPSLDVTLKNALLCSDYVIIPMTAEKWAVESLDLFNFFVRKLNLFLPIFLIITRFKKNRTHKTLFEILKTKDRFLGTISEREDLNRRIAENNNFDLNKDYIKEYENILEIFFKKI
>NZ_CP017204.1|WP_096890811.1|20428_20995_+|DUF226-domain-containing-protein
MENALEPIETVKKGKCKVECQNKERFILIEKENGKAMYHTKIMMDIYKFGVYEKKHEFRLSLRALFNGERIVEETHLYPIKEGDKFIGIFYGYRKPIKKPLIKYQINGTRKAYALARAYYMEFRFKAGSVFCYFKGLYRLLDKKRTDNHYNKVLFSMFTDLEQQVYKFYGKKYPEQGPLIKWIIKNLK
>NZ_CP017204.1|WP_096890810.1|19318_20419_+|hypothetical-protein
MKDFLITTKNPTCHNKHQHKLIYLTSTVDFLNKKDKKYTQQNILYYYNKNLKRNGLAPTTLRTMQNYLYKLEKVLKVTTNYYQHMGVNCGTEIYYKLKYPKKECYQKINKYFKERKNSRFKSRVNNHFKDNVSKNSSVNSVECLSNKNNIKEERKIKEIEKYQVIKYFNKSNFLCKEILPFLSTLNVDKDTMIKIIKNTKRVENKLLKNTNLNKSCFKEKQEKLKKILNNTQKEFEQNGYNPEQLKTNLQKVYENYKYKPHFIIENHKYSDLNNIKRKLEKSIERKKENSQQNYQNLKENIFNILIEQLKKEVNIEILKPIIKEYLNNQKKIEYNKVFGIYHLELLEIIKNEKNSLTTEEFSITAV
>NZ_CP017204.1|WP_096890809.1|17993_18941_-|ERF-family-protein
MENLSNNNNQEIQNNIQAEIDFLNDMDTLRMNLPRIDKSLKGYGYKYQDFNVIVEVIQNVIKNHNLKLGFWQFPTFVYGKNGEVPVVRTTFYSKSTGYKESLDTLIHTDKLQWNGENGSKNLNTMPQLVGAAITYFKRYALVAYLDIKSEFDTDAAPIYNNHENENSMPSKQVTVNQKQEQKKEQKQEKNQLNNFNKNLKSGKAYCYEIFRDALFNIKNWVNEGEEKNNINALIRALCTDNDDALEDLFEKNAELKSIEYWVNILKKYFNKTNRFDDLNKLKVFMSDNRDVYKTKVLKFFCMLKKERQFNYIFAV
>NZ_CP017204.1|WP_096890808.1|17302_17917_+|Mlp-family-lipoprotein
MKIINILFCLLLLVLNSCNANDNNTFNNNSVQQAESRKKRDLAQKEPPQEKITLTPDEEKMFNSLVTTFKYTIDKLNNEIQGCINGNKSKCNDFFDWLSSTDTQKQKELAQAFTTAYNFLESKRQSKAQNETFDTYIKGAIDCKNNNNNNKYGNGSNDIEQYFRGVADSIFIDKNSNEEIYKCLKDELLNENNHYAGLTANWQN
>NZ_CP017204.1|WP_096890807.1|16855_17281_+|Mlp-family-lipoprotein
MKIINILFCLFLLILNSCAANDTHTQQTKSRGKRDLTQKELTQEKPKSKEELLREKLSDDQKTHLDWLKEALESDREFDKFLEYDESKIKSALDHIKSELDKCNGKDNANDQKNTFKQVVKGALGGGIDTFTAQATTTCNN
>NZ_CP017204.1|WP_086440117.1|16040_16394_+|hypothetical-protein
MLKRLHCLLIALLLCCTTIANLPEEPKPPIIQTLKSLAKYETQLSEYVMYLVTFLAKTKVKVNDPNYPEYPYPDLSTLKDEHSITAVRHNINIYLEYIKKTKPIAEKVYNKYSQLKM
>NZ_CP017204.1|WP_002658486.1|15712_16048_+|hypothetical-protein
MQNNTIGLGLNLLSSLTNIAKTDTNIDHNYINTFSKVIDFFYKTYISTLKSMETAESTKIFEEIQDILKYNIEIIEAISTDKSKRIITSLKATRNKIMKEYIKILKRGENA
>NZ_CP017204.1|WP_010883868.1|15375_15723_+|holin,-BlyB-family
MKLSKDNVELGLTSLSTLIDIFSKFEDEFDEIAHKGFFLVYELYSHYKLIYTANMERLESALTPAINAALAPLNEKINQCIDLVNSDEKNLKISNDLKFNQEGKPIYKERTNNAK
>NZ_CP017204.1|WP_096890814.1|23302_24628_+|DUF244-domain-containing-protein
MENLSNNNNPQDNIQGDLKMISVNQQSFTGCEIFEEKFSPIKEKSKLSKIGKKLPGISSQECFRFNRNIDFSVQRNKLDKYGASEVGNILVGGAGLKDLMINRVLKYFRMSLPFEENLYMLKGKELENLGFREFVKAYGDNIDILYKNKYANGVDKYNYFKKMGSSETLVGSTIDGWFINNNGDLELLEIKSSDSNYMSSAIAEYNKNGNFLSSKYFFKYYVQAQMQLACTGLEYCNLFFLIDAAPINCKIKRDEALISKVFEFVNKCELEIINLKKDIYSNYREEYLMAHNFNEDTFIKLVEDLVERSDFYSSGVEFDWAREFIEYVDCADLEIKDNQSAENLAYDLMEINSLQKELNRIQNENKKREKPIKDRLKMLIYNITNTYPLIEQLNYKFGEFVFTLDPKKRAISDRLKGLLSTSGTVFSPSNIAFANSVSVPM
>NZ_CP017204.1|WP_012621296.1|24680_25106_+|DUF261-domain-containing-protein
MLINKIKQDNRTLRLEIQKWGCYFLCLHYYTSLFKKREFSAYEINAAYYRFIGLGYIKSNCFIINPCMILNYYGIRSSVRYESLNYLGAANEFEISEVKIDKVNGYHFIATKNKEILYDSLDLKPRGKIFKVTSKRIFKLK
>NZ_CP017204.1|WP_096890815.1|25111_25906_-|site-specific-integrase
MDINNSFNLNNFNMDFTLKLFQEYQNALNKNKILENENKILKSLENSPKRKKKNSKPTSKFYLTPKSIKLILKCAKTLKQIDPISGWFVHLLLISGCRGTEMQKVKMQDISTFLSKTGKTLYNIKVNVAKKRNASCIREIVINSEEFEAIQTAHKNHFQEKTLDSRRTYLFQKSKHKFKDNQIDIVHISKKFKNLLKKSGFRVNKSLHLCRNLFISNLKSNGYNSFQIKELMKYSSTNEIDNIYGLSSANKIQAYECAKKCLKL
>NZ_CP017204.1|WP_096890816.1|26336_27359_+|laminin-binding-protein-ErpX
MNKKMKIFIICAVFVLISSCKIDATGKDATGKDATGKDATGKNAEQNIKGKVQGFLEKILDPVKDKIASNGPIADELAKKLQEEEKVNNGEEENDKAVFLGEESKEDEEENEQAVNLEEKNAEEDKKVVNLEEKELEVKKETEEDEDKEEIEKQKQEVEKAQERKQRQEEKKRKKQEQQEEKKRKRQEQRKERRAKNKIKKLADKIDEISWNIDGIESQTSVKPKAVIDKITGPVYDYFTDDNKKAIYKTWGDLEDEEGEGLGKLLKELSDTRDELRTKLNKDNKKYYAHENEPPLKENVDVSEIKEDLEKVKSGLEKVKEYLKDNSKFEEIKGYISDSQ
>NZ_CP017204.1|WP_096890817.1|27552_28095_+|DUF643-domain-containing-protein
MTYTNEISDFDDNLYKKTKKEIGKLINKLYLTSQITLKQKRQIYSAVERMQKYVIKTGKSVLLESEKEFVKDTLKRKNLIKKFKSFKVDFSYKERMLEKCLERLGEDKSIEFLIFVYQILNGIREKVLELDFQIDAIEEFRDILFLSIHYYDKRLFTSKNLMNEMKDFSEKVELIYSNMQ
>NZ_CP017204.1|WP_032492911.1|28276_28843_+|DUF603-domain-containing-protein
MKKVKRPFDDYVVYFREGSLNDREIAEKLGVSKVNVWRMRQKWESGESIVNQDARVTISEDTLEHLLSQTFRSEVNARKVISELDIECANLKLGFIRAFKQYSSVELTGMRTKIENLRAEIDALNKASSKKNKQFVNGEINFLKSELDEYIKECSIREMELYYECMKKLTTANEAESKSNYKNSKGHK
>NZ_CP017204.1|WP_096890818.1|28839_30192_+|PBSX-family-phage-terminase-large-subunit
MNLYQTKLFTTLQKEYKNKYGVDISQFVKLTNSSINFDKFEEKQLTLKQKNVIKSIKKNNEKKIILSGGIASGKTYLACYLFLKSLIENKKLYSSDTNNFIIGNSQRSVEVNVLGQFEKLCKLLKIPYIPRHTNNSYILIDSLRINLYGGDKASDFERFRGSNSALIFVNEATTLHKQTLEEVLKRLRCGQETIIFDTNPDHPEHYFKTDYIDNIATFKTYNFTTYDNVLLSKGFIETQEKLYKDIPSYKARVLLGEWIASTDSIFTQINITDDYVFTSPIAYLDPAFSVGGDNTALCVMERVDDKYYAFVFQDQRPANDPYIMNMVKTVIENFNVHTLYLEDRDNTKGAGGLTREYILLRSNISQYFRIVPVKPKSNKFSRITTLITPFTYKKLYITKYCSSSVFNDIYSYKGDNKTHDDALDAISAAYLMLSLGYRERSVHFGNQRFL

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Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NZ_CP017206.1	17338-17388	2	0.961

1. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to position: 17338-17388, mismatch: 2, identity: 0.961

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ctaaaatagacaatgtagaaaagaatttaaatctaaaaatagatagtttag	Protospacer
**************** ****************************.*****

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity
NZ_CP017204_1	1.1\|22489\|53\|NZ_CP017204\|PILER-CR	22489-22541	53	NZ_CP019920	Borreliella burgdorferi strain PAbe plasmid p_cp32-3, complete sequence	18321-18373	0	1.0
NZ_CP017204_1	1.1\|22489\|53\|NZ_CP017204\|PILER-CR	22489-22541	53	NZ_CP019926	Borreliella burgdorferi strain PAbe plasmid p_lp56, complete sequence	26052-26104	0	1.0
NZ_CP017204_1	1.1\|22489\|53\|NZ_CP017204\|PILER-CR	22489-22541	53	NC_017427	Borreliella burgdorferi JD1 plasmid JD1 cp32-3, complete sequence	22324-22376	0	1.0
NZ_CP017204_1	1.1\|22489\|53\|NZ_CP017204\|PILER-CR	22489-22541	53	NC_017424	Borreliella burgdorferi N40 plasmid N40_cp32-10, complete sequence	22474-22526	0	1.0
NZ_CP017204_1	1.1\|22489\|53\|NZ_CP017204\|PILER-CR	22489-22541	53	NC_011720	Borreliella burgdorferi ZS7 plasmid ZS7_cp32-3+10, complete sequence	13004-13056	0	1.0
NZ_CP017204_1	1.1\|22489\|53\|NZ_CP017204\|PILER-CR	22489-22541	53	NC_000949	Borreliella burgdorferi B31 plasmid cp32-3, complete sequence	22755-22807	0	1.0
NZ_CP017204_1	1.1\|22489\|53\|NZ_CP017204\|PILER-CR	22489-22541	53	NC_000956	Borreliella burgdorferi B31 plasmid lp56, complete sequence	26052-26104	0	1.0
NZ_CP017204_1	1.1\|22489\|53\|NZ_CP017204\|PILER-CR	22489-22541	53	NZ_CP019847	Borreliella burgdorferi plasmid cp32-3, complete sequence	22755-22807	0	1.0
NZ_CP017204_1	1.1\|22489\|53\|NZ_CP017204\|PILER-CR	22489-22541	53	NZ_CP019855	Borreliella burgdorferi plasmid lp56, complete sequence	26052-26104	0	1.0
NZ_CP017204_1	1.1\|22489\|53\|NZ_CP017204\|PILER-CR	22489-22541	53	NZ_CP019758	Borreliella burgdorferi strain B31_NRZ isolate B31 plasmid p_cp32-3, complete sequence	22754-22806	0	1.0
NZ_CP017204_1	1.1\|22489\|53\|NZ_CP017204\|PILER-CR	22489-22541	53	NZ_CP019766	Borreliella burgdorferi strain B31_NRZ isolate B31 plasmid p_lp56, complete sequence	26032-26084	0	1.0
NZ_CP017204_1	1.1\|22489\|53\|NZ_CP017204\|PILER-CR	22489-22541	53	NZ_CP017204	Borreliella burgdorferi strain B331 plasmid B331_cp32_10, complete sequence	22469-22521	0	1.0
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NZ_CP019920	Borreliella burgdorferi strain PAbe plasmid p_cp32-3, complete sequence	18379-18429	0	1.0
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NC_019006	Borrelia burgdorferi 297 plasmid 297_cp32-3, complete sequence	22923-22973	0	1.0
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NC_017424	Borreliella burgdorferi N40 plasmid N40_cp32-10, complete sequence	22532-22582	0	1.0
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NC_011720	Borreliella burgdorferi ZS7 plasmid ZS7_cp32-3+10, complete sequence	13062-13112	0	1.0
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NC_000949	Borreliella burgdorferi B31 plasmid cp32-3, complete sequence	22813-22863	0	1.0
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NZ_CP015800	Borrelia mayonii strain MN14-1539 plasmid cp32-3, complete sequence	22157-22207	0	1.0
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NZ_CP019847	Borreliella burgdorferi plasmid cp32-3, complete sequence	22813-22863	0	1.0
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NZ_CP019758	Borreliella burgdorferi strain B31_NRZ isolate B31 plasmid p_cp32-3, complete sequence	22812-22862	0	1.0
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NZ_CP017204	Borreliella burgdorferi strain B331 plasmid B331_cp32_10, complete sequence	22560-22610	0	1.0
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NZ_CP015784	Borrelia mayonii strain MN14-1420 plasmid cp32-3, complete sequence	22124-22174	0	1.0
NZ_CP017204_1	1.1\|22489\|53\|NZ_CP017204\|PILER-CR	22489-22541	53	NC_015904	Borreliella bissettii DN127 plasmid cp32-4, complete sequence	16799-16851	1	0.981
NZ_CP017204_1	1.1\|22489\|53\|NZ_CP017204\|PILER-CR	22489-22541	53	NC_015908	Borreliella bissettii DN127 plasmid cp32-3, complete sequence	17448-17500	1	0.981
NZ_CP017204_1	1.1\|22489\|53\|NZ_CP017204\|PILER-CR	22489-22541	53	NZ_CP015800	Borrelia mayonii strain MN14-1539 plasmid cp32-3, complete sequence	22066-22118	1	0.981
NZ_CP017204_1	1.1\|22489\|53\|NZ_CP017204\|PILER-CR	22489-22541	53	NZ_CP015784	Borrelia mayonii strain MN14-1420 plasmid cp32-3, complete sequence	22066-22118	1	0.981
NZ_CP017204_1	1.1\|22489\|53\|NZ_CP017204\|PILER-CR	22489-22541	53	NC_015906	Borreliella bissettii DN127 plasmid cp32-quad, complete sequence	40024-40076	1	0.981
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NZ_CP019926	Borreliella burgdorferi strain PAbe plasmid p_lp56, complete sequence	26143-26193	1	0.98
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NC_017427	Borreliella burgdorferi JD1 plasmid JD1 cp32-3, complete sequence	22382-22432	1	0.98
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NC_000956	Borreliella burgdorferi B31 plasmid lp56, complete sequence	26143-26193	1	0.98
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NZ_CP019855	Borreliella burgdorferi plasmid lp56, complete sequence	26143-26193	1	0.98
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NZ_CP019766	Borreliella burgdorferi strain B31_NRZ isolate B31 plasmid p_lp56, complete sequence	26123-26173	1	0.98
NZ_CP017204_1	1.1\|22489\|53\|NZ_CP017204\|PILER-CR	22489-22541	53	NZ_CP019918	Borreliella burgdorferi strain PAbe plasmid p_cp32-5-1, complete sequence	46353-46405	2	0.962
NZ_CP017204_1	1.1\|22489\|53\|NZ_CP017204\|PILER-CR	22489-22541	53	NC_019003	Borrelia burgdorferi 297 plasmid 297_cp32-1, complete sequence	22584-22636	2	0.962
NZ_CP017204_1	1.1\|22489\|53\|NZ_CP017204\|PILER-CR	22489-22541	53	NC_017394	Borreliella burgdorferi JD1 plasmid JD1 cp32-1+5, complete sequence	22679-22731	2	0.962
NZ_CP017204_1	1.1\|22489\|53\|NZ_CP017204\|PILER-CR	22489-22541	53	NZ_CP031408	Borreliella burgdorferi strain MM1 plasmid plsm_cp32-1, complete sequence	11880-11932	2	0.962
NZ_CP017204_1	1.1\|22489\|53\|NZ_CP017204\|PILER-CR	22489-22541	53	NC_000948	Borreliella burgdorferi B31 plasmid cp32-1, complete sequence	22401-22453	2	0.962
NZ_CP017204_1	1.1\|22489\|53\|NZ_CP017204\|PILER-CR	22489-22541	53	NZ_CP019846	Borreliella burgdorferi plasmid cp32-1, complete sequence	22377-22429	2	0.962
NZ_CP017204_1	1.1\|22489\|53\|NZ_CP017204\|PILER-CR	22489-22541	53	NZ_CP019756	Borreliella burgdorferi strain B31_NRZ isolate B31 plasmid p_cp32-1_1, complete sequence	53069-53121	2	0.962
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NZ_CP019920	Borreliella burgdorferi strain PAbe plasmid p_cp32-3, complete sequence	18466-18516	2	0.961
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NC_019006	Borrelia burgdorferi 297 plasmid 297_cp32-3, complete sequence	23010-23060	2	0.961
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NC_011720	Borreliella burgdorferi ZS7 plasmid ZS7_cp32-3+10, complete sequence	13149-13199	2	0.961
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NC_000949	Borreliella burgdorferi B31 plasmid cp32-3, complete sequence	22900-22950	2	0.961
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NZ_CP015800	Borrelia mayonii strain MN14-1539 plasmid cp32-3, complete sequence	16694-16744	2	0.961
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NZ_CP019847	Borreliella burgdorferi plasmid cp32-3, complete sequence	22900-22950	2	0.961
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NZ_CP019758	Borreliella burgdorferi strain B31_NRZ isolate B31 plasmid p_cp32-3, complete sequence	22899-22949	2	0.961
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NZ_CP015784	Borrelia mayonii strain MN14-1420 plasmid cp32-3, complete sequence	16694-16744	2	0.961
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NC_017427	Borreliella burgdorferi JD1 plasmid JD1 cp32-3, complete sequence	22469-22519	2	0.961
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NC_018982	Borrelia burgdorferi 297 plasmid 297_cp32-9, complete sequence	8492-8542	2	0.961
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NC_018982	Borrelia burgdorferi 297 plasmid 297_cp32-9, complete sequence	14255-14305	2	0.961
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NC_019003	Borrelia burgdorferi 297 plasmid 297_cp32-1, complete sequence	16749-16799	2	0.961
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NC_017397	Borreliella burgdorferi JD1 plasmid JD1 cp32-9, complete sequence	16778-16828	2	0.961
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NC_015906	Borreliella bissettii DN127 plasmid cp32-quad, complete sequence	10762-10812	2	0.961
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NC_004971	Borrelia burgdorferi 297 plasmid cp18-2, complete sequence	8475-8525	2	0.961
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NC_004971	Borrelia burgdorferi 297 plasmid cp18-2, complete sequence	14237-14287	2	0.961
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NC_011731	Borreliella burgdorferi ZS7 plasmid ZS7_cp32-1, complete sequence	16685-16735	2	0.961
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NZ_CP017206	Borreliella burgdorferi strain B331 plasmid B331_cp32_4, complete sequence	17338-17388	2	0.961
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NC_015909	Borreliella bissettii DN127 plasmid cp32-5, complete sequence	16761-16811	6	0.882
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NZ_CP019920	Borreliella burgdorferi strain PAbe plasmid p_cp32-3, complete sequence	18553-18603	7	0.863
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NC_019006	Borrelia burgdorferi 297 plasmid 297_cp32-3, complete sequence	23097-23147	7	0.863
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NC_017424	Borreliella burgdorferi N40 plasmid N40_cp32-10, complete sequence	22619-22669	7	0.863
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NC_000949	Borreliella burgdorferi B31 plasmid cp32-3, complete sequence	22987-23037	7	0.863
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NZ_CP015800	Borrelia mayonii strain MN14-1539 plasmid cp32-3, complete sequence	22244-22294	7	0.863
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NZ_CP019847	Borreliella burgdorferi plasmid cp32-3, complete sequence	22987-23037	7	0.863
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NZ_CP019758	Borreliella burgdorferi strain B31_NRZ isolate B31 plasmid p_cp32-3, complete sequence	22986-23036	7	0.863
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NZ_CP015784	Borrelia mayonii strain MN14-1420 plasmid cp32-3, complete sequence	22211-22261	7	0.863
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NZ_CP019926	Borreliella burgdorferi strain PAbe plasmid p_lp56, complete sequence	26230-26280	7	0.863
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NC_017427	Borreliella burgdorferi JD1 plasmid JD1 cp32-3, complete sequence	22556-22606	7	0.863
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NC_000956	Borreliella burgdorferi B31 plasmid lp56, complete sequence	26230-26280	7	0.863
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NZ_CP019855	Borreliella burgdorferi plasmid lp56, complete sequence	26230-26280	7	0.863
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NZ_CP019766	Borreliella burgdorferi strain B31_NRZ isolate B31 plasmid p_lp56, complete sequence	26210-26260	7	0.863
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NC_015910	Borreliella bissettii DN127 plasmid cp32-7, complete sequence	23005-23055	7	0.863
NZ_CP017204_1	1.2\|22581\|51\|NZ_CP017204\|PILER-CR	22581-22631	51	NC_015910	Borreliella bissettii DN127 plasmid cp32-7, complete sequence	22777-22827	8	0.843
NZ_CP017204_1	1.1\|22489\|53\|NZ_CP017204\|PILER-CR	22489-22541	53	NC_015903	Borreliella bissettii DN127 plasmid cp32-11, complete sequence	17192-17244	12	0.774
NZ_CP017204_1	1.1\|22489\|53\|NZ_CP017204\|PILER-CR	22489-22541	53	NC_015903	Borreliella bissettii DN127 plasmid cp32-11, complete sequence	17225-17277	12	0.774
NZ_CP017204_1	1.1\|22489\|53\|NZ_CP017204\|PILER-CR	22489-22541	53	NZ_CP015798	Borrelia mayonii strain MN14-1539 plasmid cp32-1, complete sequence	16833-16885	12	0.774

1. spacer 1.1|22489|53|NZ_CP017204|PILER-CR matches to NZ_CP019920 (Borreliella burgdorferi strain PAbe plasmid p_cp32-3, complete sequence) position: , mismatch: 0, identity: 1.0

caaaaggatatatctaatttagacattaagattgatactgtagaaaagaattt	CRISPR spacer
caaaaggatatatctaatttagacattaagattgatactgtagaaaagaattt	Protospacer
*****************************************************

2. spacer 1.1|22489|53|NZ_CP017204|PILER-CR matches to NZ_CP019926 (Borreliella burgdorferi strain PAbe plasmid p_lp56, complete sequence) position: , mismatch: 0, identity: 1.0

caaaaggatatatctaatttagacattaagattgatactgtagaaaagaattt	CRISPR spacer
caaaaggatatatctaatttagacattaagattgatactgtagaaaagaattt	Protospacer
*****************************************************

3. spacer 1.1|22489|53|NZ_CP017204|PILER-CR matches to NC_017427 (Borreliella burgdorferi JD1 plasmid JD1 cp32-3, complete sequence) position: , mismatch: 0, identity: 1.0

caaaaggatatatctaatttagacattaagattgatactgtagaaaagaattt	CRISPR spacer
caaaaggatatatctaatttagacattaagattgatactgtagaaaagaattt	Protospacer
*****************************************************

4. spacer 1.1|22489|53|NZ_CP017204|PILER-CR matches to NC_017424 (Borreliella burgdorferi N40 plasmid N40_cp32-10, complete sequence) position: , mismatch: 0, identity: 1.0

caaaaggatatatctaatttagacattaagattgatactgtagaaaagaattt	CRISPR spacer
caaaaggatatatctaatttagacattaagattgatactgtagaaaagaattt	Protospacer
*****************************************************

5. spacer 1.1|22489|53|NZ_CP017204|PILER-CR matches to NC_011720 (Borreliella burgdorferi ZS7 plasmid ZS7_cp32-3+10, complete sequence) position: , mismatch: 0, identity: 1.0

caaaaggatatatctaatttagacattaagattgatactgtagaaaagaattt	CRISPR spacer
caaaaggatatatctaatttagacattaagattgatactgtagaaaagaattt	Protospacer
*****************************************************

6. spacer 1.1|22489|53|NZ_CP017204|PILER-CR matches to NC_000949 (Borreliella burgdorferi B31 plasmid cp32-3, complete sequence) position: , mismatch: 0, identity: 1.0

caaaaggatatatctaatttagacattaagattgatactgtagaaaagaattt	CRISPR spacer
caaaaggatatatctaatttagacattaagattgatactgtagaaaagaattt	Protospacer
*****************************************************

7. spacer 1.1|22489|53|NZ_CP017204|PILER-CR matches to NC_000956 (Borreliella burgdorferi B31 plasmid lp56, complete sequence) position: , mismatch: 0, identity: 1.0

caaaaggatatatctaatttagacattaagattgatactgtagaaaagaattt	CRISPR spacer
caaaaggatatatctaatttagacattaagattgatactgtagaaaagaattt	Protospacer
*****************************************************

8. spacer 1.1|22489|53|NZ_CP017204|PILER-CR matches to NZ_CP019847 (Borreliella burgdorferi plasmid cp32-3, complete sequence) position: , mismatch: 0, identity: 1.0

caaaaggatatatctaatttagacattaagattgatactgtagaaaagaattt	CRISPR spacer
caaaaggatatatctaatttagacattaagattgatactgtagaaaagaattt	Protospacer
*****************************************************

9. spacer 1.1|22489|53|NZ_CP017204|PILER-CR matches to NZ_CP019855 (Borreliella burgdorferi plasmid lp56, complete sequence) position: , mismatch: 0, identity: 1.0

caaaaggatatatctaatttagacattaagattgatactgtagaaaagaattt	CRISPR spacer
caaaaggatatatctaatttagacattaagattgatactgtagaaaagaattt	Protospacer
*****************************************************

10. spacer 1.1|22489|53|NZ_CP017204|PILER-CR matches to NZ_CP019758 (Borreliella burgdorferi strain B31_NRZ isolate B31 plasmid p_cp32-3, complete sequence) position: , mismatch: 0, identity: 1.0

caaaaggatatatctaatttagacattaagattgatactgtagaaaagaattt	CRISPR spacer
caaaaggatatatctaatttagacattaagattgatactgtagaaaagaattt	Protospacer
*****************************************************

11. spacer 1.1|22489|53|NZ_CP017204|PILER-CR matches to NZ_CP019766 (Borreliella burgdorferi strain B31_NRZ isolate B31 plasmid p_lp56, complete sequence) position: , mismatch: 0, identity: 1.0

caaaaggatatatctaatttagacattaagattgatactgtagaaaagaattt	CRISPR spacer
caaaaggatatatctaatttagacattaagattgatactgtagaaaagaattt	Protospacer
*****************************************************

12. spacer 1.1|22489|53|NZ_CP017204|PILER-CR matches to NZ_CP017204 (Borreliella burgdorferi strain B331 plasmid B331_cp32_10, complete sequence) position: , mismatch: 0, identity: 1.0

caaaaggatatatctaatttagacattaagattgatactgtagaaaagaattt	CRISPR spacer
caaaaggatatatctaatttagacattaagattgatactgtagaaaagaattt	Protospacer
*****************************************************

13. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NZ_CP019920 (Borreliella burgdorferi strain PAbe plasmid p_cp32-3, complete sequence) position: , mismatch: 0, identity: 1.0

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	Protospacer
***************************************************

14. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NC_019006 (Borrelia burgdorferi 297 plasmid 297_cp32-3, complete sequence) position: , mismatch: 0, identity: 1.0

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	Protospacer
***************************************************

15. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NC_017424 (Borreliella burgdorferi N40 plasmid N40_cp32-10, complete sequence) position: , mismatch: 0, identity: 1.0

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	Protospacer
***************************************************

16. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NC_011720 (Borreliella burgdorferi ZS7 plasmid ZS7_cp32-3+10, complete sequence) position: , mismatch: 0, identity: 1.0

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	Protospacer
***************************************************

17. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NC_000949 (Borreliella burgdorferi B31 plasmid cp32-3, complete sequence) position: , mismatch: 0, identity: 1.0

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	Protospacer
***************************************************

18. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NZ_CP015800 (Borrelia mayonii strain MN14-1539 plasmid cp32-3, complete sequence) position: , mismatch: 0, identity: 1.0

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	Protospacer
***************************************************

19. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NZ_CP019847 (Borreliella burgdorferi plasmid cp32-3, complete sequence) position: , mismatch: 0, identity: 1.0

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	Protospacer
***************************************************

20. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NZ_CP019758 (Borreliella burgdorferi strain B31_NRZ isolate B31 plasmid p_cp32-3, complete sequence) position: , mismatch: 0, identity: 1.0

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	Protospacer
***************************************************

21. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NZ_CP017204 (Borreliella burgdorferi strain B331 plasmid B331_cp32_10, complete sequence) position: , mismatch: 0, identity: 1.0

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	Protospacer
***************************************************

22. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NZ_CP015784 (Borrelia mayonii strain MN14-1420 plasmid cp32-3, complete sequence) position: , mismatch: 0, identity: 1.0

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	Protospacer
***************************************************

23. spacer 1.1|22489|53|NZ_CP017204|PILER-CR matches to NC_015904 (Borreliella bissettii DN127 plasmid cp32-4, complete sequence) position: , mismatch: 1, identity: 0.981

caaaaggatatatctaatttagacattaagattgatactgtagaaaagaattt	CRISPR spacer
caaaaggatatatctaatttagacattaagatagatactgtagaaaagaattt	Protospacer
******************************** ********************

24. spacer 1.1|22489|53|NZ_CP017204|PILER-CR matches to NC_015908 (Borreliella bissettii DN127 plasmid cp32-3, complete sequence) position: , mismatch: 1, identity: 0.981

caaaaggatatatctaatttagacattaagattgatactgtagaaaagaattt	CRISPR spacer
caaaaggatatatctaatttagacattaagatagatactgtagaaaagaattt	Protospacer
******************************** ********************

25. spacer 1.1|22489|53|NZ_CP017204|PILER-CR matches to NZ_CP015800 (Borrelia mayonii strain MN14-1539 plasmid cp32-3, complete sequence) position: , mismatch: 1, identity: 0.981

caaaaggatatatctaatttagacattaagattgatactgtagaaaagaattt	CRISPR spacer
caaaaggatatatctaatttagacattaagattgatgctgtagaaaagaattt	Protospacer
************************************.****************

26. spacer 1.1|22489|53|NZ_CP017204|PILER-CR matches to NZ_CP015784 (Borrelia mayonii strain MN14-1420 plasmid cp32-3, complete sequence) position: , mismatch: 1, identity: 0.981

caaaaggatatatctaatttagacattaagattgatactgtagaaaagaattt	CRISPR spacer
caaaaggatatatctaatttagacattaagattgatgctgtagaaaagaattt	Protospacer
************************************.****************

27. spacer 1.1|22489|53|NZ_CP017204|PILER-CR matches to NC_015906 (Borreliella bissettii DN127 plasmid cp32-quad, complete sequence) position: , mismatch: 1, identity: 0.981

caaaaggatatatctaatttagacattaagattgatactgtagaaaagaattt	CRISPR spacer
caaaaggatatatctaatttagacattaagatagatactgtagaaaagaattt	Protospacer
******************************** ********************

28. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NZ_CP019926 (Borreliella burgdorferi strain PAbe plasmid p_lp56, complete sequence) position: , mismatch: 1, identity: 0.98

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ctaaaatagacaatgtagaaaagaatttaaatctaaaaatagataatttag	Protospacer
**************** **********************************

29. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NC_017427 (Borreliella burgdorferi JD1 plasmid JD1 cp32-3, complete sequence) position: , mismatch: 1, identity: 0.98

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ctcaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	Protospacer
** ************************************************

30. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NC_000956 (Borreliella burgdorferi B31 plasmid lp56, complete sequence) position: , mismatch: 1, identity: 0.98

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ctaaaatagacaatgtagaaaagaatttaaatctaaaaatagataatttag	Protospacer
**************** **********************************

31. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NZ_CP019855 (Borreliella burgdorferi plasmid lp56, complete sequence) position: , mismatch: 1, identity: 0.98

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ctaaaatagacaatgtagaaaagaatttaaatctaaaaatagataatttag	Protospacer
**************** **********************************

32. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NZ_CP019766 (Borreliella burgdorferi strain B31_NRZ isolate B31 plasmid p_lp56, complete sequence) position: , mismatch: 1, identity: 0.98

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ctaaaatagacaatgtagaaaagaatttaaatctaaaaatagataatttag	Protospacer
**************** **********************************

33. spacer 1.1|22489|53|NZ_CP017204|PILER-CR matches to NZ_CP019918 (Borreliella burgdorferi strain PAbe plasmid p_cp32-5-1, complete sequence) position: , mismatch: 2, identity: 0.962

caaaaggatatatctaatttagacattaagattgatactgtagaaaagaattt	CRISPR spacer
caaaaggatatatctaatttagacatcaagattgataatgtagaaaagaattt	Protospacer
**************************.********** ***************

34. spacer 1.1|22489|53|NZ_CP017204|PILER-CR matches to NC_019003 (Borrelia burgdorferi 297 plasmid 297_cp32-1, complete sequence) position: , mismatch: 2, identity: 0.962

caaaaggatatatctaatttagacattaagattgatactgtagaaaagaattt	CRISPR spacer
caaaaggatatatctaatttagacatcaagattgataatgtagaaaagaattt	Protospacer
**************************.********** ***************

35. spacer 1.1|22489|53|NZ_CP017204|PILER-CR matches to NC_017394 (Borreliella burgdorferi JD1 plasmid JD1 cp32-1+5, complete sequence) position: , mismatch: 2, identity: 0.962

caaaaggatatatctaatttagacattaagattgatactgtagaaaagaattt	CRISPR spacer
caaaaggatatatctaatttagacatcaagattgataatgtagaaaagaattt	Protospacer
**************************.********** ***************

36. spacer 1.1|22489|53|NZ_CP017204|PILER-CR matches to NZ_CP031408 (Borreliella burgdorferi strain MM1 plasmid plsm_cp32-1, complete sequence) position: , mismatch: 2, identity: 0.962

caaaaggatatatctaatttagacattaagattgatactgtagaaaagaattt	CRISPR spacer
caaaaggatatatctaatttagacatcaagattgataatgtagaaaagaattt	Protospacer
**************************.********** ***************

37. spacer 1.1|22489|53|NZ_CP017204|PILER-CR matches to NC_000948 (Borreliella burgdorferi B31 plasmid cp32-1, complete sequence) position: , mismatch: 2, identity: 0.962

caaaaggatatatctaatttagacattaagattgatactgtagaaaagaattt	CRISPR spacer
caaaaggatatatctaatttagacatcaagattgataatgtagaaaagaattt	Protospacer
**************************.********** ***************

38. spacer 1.1|22489|53|NZ_CP017204|PILER-CR matches to NZ_CP019846 (Borreliella burgdorferi plasmid cp32-1, complete sequence) position: , mismatch: 2, identity: 0.962

caaaaggatatatctaatttagacattaagattgatactgtagaaaagaattt	CRISPR spacer
caaaaggatatatctaatttagacatcaagattgataatgtagaaaagaattt	Protospacer
**************************.********** ***************

39. spacer 1.1|22489|53|NZ_CP017204|PILER-CR matches to NZ_CP019756 (Borreliella burgdorferi strain B31_NRZ isolate B31 plasmid p_cp32-1_1, complete sequence) position: , mismatch: 2, identity: 0.962

caaaaggatatatctaatttagacattaagattgatactgtagaaaagaattt	CRISPR spacer
caaaaggatatatctaatttagacatcaagattgataatgtagaaaagaattt	Protospacer
**************************.********** ***************

40. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NZ_CP019920 (Borreliella burgdorferi strain PAbe plasmid p_cp32-3, complete sequence) position: , mismatch: 2, identity: 0.961

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ctaaaatagacaatgttgaaaagaatttaaacctaaaaatggataatttag	Protospacer
*******************************.********.**********

41. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NC_019006 (Borrelia burgdorferi 297 plasmid 297_cp32-3, complete sequence) position: , mismatch: 2, identity: 0.961

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ctaaaatagacaatgttgaaaagaatttaaacctaaaaatggataatttag	Protospacer
*******************************.********.**********

42. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NC_011720 (Borreliella burgdorferi ZS7 plasmid ZS7_cp32-3+10, complete sequence) position: , mismatch: 2, identity: 0.961

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ctaaaatagacaatgttgaaaagaatttaaacctaaaaatggataatttag	Protospacer
*******************************.********.**********

43. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NC_000949 (Borreliella burgdorferi B31 plasmid cp32-3, complete sequence) position: , mismatch: 2, identity: 0.961

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ctaaaatagacaatgttgaaaagaatttaaacctaaaaatggataatttag	Protospacer
*******************************.********.**********

44. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NZ_CP015800 (Borrelia mayonii strain MN14-1539 plasmid cp32-3, complete sequence) position: , mismatch: 2, identity: 0.961

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ctaaaatagacaatgtagaaaagaatttaaatctaaaaatagatagtttag	Protospacer
**************** ****************************.*****

45. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NZ_CP019847 (Borreliella burgdorferi plasmid cp32-3, complete sequence) position: , mismatch: 2, identity: 0.961

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ctaaaatagacaatgttgaaaagaatttaaacctaaaaatggataatttag	Protospacer
*******************************.********.**********

46. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NZ_CP019758 (Borreliella burgdorferi strain B31_NRZ isolate B31 plasmid p_cp32-3, complete sequence) position: , mismatch: 2, identity: 0.961

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ctaaaatagacaatgttgaaaagaatttaaacctaaaaatggataatttag	Protospacer
*******************************.********.**********

47. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NZ_CP015784 (Borrelia mayonii strain MN14-1420 plasmid cp32-3, complete sequence) position: , mismatch: 2, identity: 0.961

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ctaaaatagacaatgtagaaaagaatttaaatctaaaaatagatagtttag	Protospacer
**************** ****************************.*****

48. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NC_017427 (Borreliella burgdorferi JD1 plasmid JD1 cp32-3, complete sequence) position: , mismatch: 2, identity: 0.961

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ctaaaatagacaatgttgaaaagaatttaaacctaaaaatggataatttag	Protospacer
*******************************.********.**********

49. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NC_018982 (Borrelia burgdorferi 297 plasmid 297_cp32-9, complete sequence) position: , mismatch: 2, identity: 0.961

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ctaaaatagacaatgtagaaaagaatttaaatctaaaaatagatagtttag	Protospacer
**************** ****************************.*****

50. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NC_018982 (Borrelia burgdorferi 297 plasmid 297_cp32-9, complete sequence) position: , mismatch: 2, identity: 0.961

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ccaaaatagacaatgttgaaaagaatttaaatctaaaaatggataatttag	Protospacer
*.**************************************.**********

51. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NC_019003 (Borrelia burgdorferi 297 plasmid 297_cp32-1, complete sequence) position: , mismatch: 2, identity: 0.961

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ctaaaatagacaatgtagaaaagaatttaaatctaaaaatagatagtttag	Protospacer
**************** ****************************.*****

52. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NC_017397 (Borreliella burgdorferi JD1 plasmid JD1 cp32-9, complete sequence) position: , mismatch: 2, identity: 0.961

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ctaaaatagacaatgtagaaaagaatttaaatctaaaaatagatagtttag	Protospacer
**************** ****************************.*****

53. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NC_015906 (Borreliella bissettii DN127 plasmid cp32-quad, complete sequence) position: , mismatch: 2, identity: 0.961

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ctaaaatagacaatgtagaaaagaatttaaatctaaaaatagatagtttag	Protospacer
**************** ****************************.*****

54. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NC_004971 (Borrelia burgdorferi 297 plasmid cp18-2, complete sequence) position: , mismatch: 2, identity: 0.961

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ctaaaatagacaatgtagaaaagaatttaaatctaaaaatagatagtttag	Protospacer
**************** ****************************.*****

55. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NC_004971 (Borrelia burgdorferi 297 plasmid cp18-2, complete sequence) position: , mismatch: 2, identity: 0.961

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ccaaaatagacaatgttgaaaagaatttaaatctaaaaatggataatttag	Protospacer
*.**************************************.**********

56. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NC_011731 (Borreliella burgdorferi ZS7 plasmid ZS7_cp32-1, complete sequence) position: , mismatch: 2, identity: 0.961

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ctaaaatagacaatgtagaaaagaatttaaatctaaaaatagatagtttag	Protospacer
**************** ****************************.*****

57. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NZ_CP017206 (Borreliella burgdorferi strain B331 plasmid B331_cp32_4, complete sequence) position: , mismatch: 2, identity: 0.961

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ctaaaatagacaatgtagaaaagaatttaaatctaaaaatagatagtttag	Protospacer
**************** ****************************.*****

58. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NC_015909 (Borreliella bissettii DN127 plasmid cp32-5, complete sequence) position: , mismatch: 6, identity: 0.882

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ctaaaatagataatgtagaaaagaatttaaatctaaaaatagatagtgtta	Protospacer
**********.***** ****************************.* * .

59. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NZ_CP019920 (Borreliella burgdorferi strain PAbe plasmid p_cp32-3, complete sequence) position: , mismatch: 7, identity: 0.863

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ctaaaatagacaatgttgaaaagaatttaaacctaaaaatggatggcttga	Protospacer
*******************************.********.***...**..

60. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NC_019006 (Borrelia burgdorferi 297 plasmid 297_cp32-3, complete sequence) position: , mismatch: 7, identity: 0.863

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ctaaaatagacaatgttgaaaagaatttaaacctaaaaatggatggcttga	Protospacer
*******************************.********.***...**..

61. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NC_017424 (Borreliella burgdorferi N40 plasmid N40_cp32-10, complete sequence) position: , mismatch: 7, identity: 0.863

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ctaaaatagacaatgttgaaaagaatttaaacctaaaaatggatggcttga	Protospacer
*******************************.********.***...**..

62. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NC_000949 (Borreliella burgdorferi B31 plasmid cp32-3, complete sequence) position: , mismatch: 7, identity: 0.863

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ctaaaatagacaatgttgaaaagaatttaaacctaaaaatggatggcttga	Protospacer
*******************************.********.***...**..

63. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NZ_CP015800 (Borrelia mayonii strain MN14-1539 plasmid cp32-3, complete sequence) position: , mismatch: 7, identity: 0.863

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ctaaaatagacaatgttgaaaagaatttaaacctaaaaatggatggcttga	Protospacer
*******************************.********.***...**..

64. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NZ_CP019847 (Borreliella burgdorferi plasmid cp32-3, complete sequence) position: , mismatch: 7, identity: 0.863

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ctaaaatagacaatgttgaaaagaatttaaacctaaaaatggatggcttga	Protospacer
*******************************.********.***...**..

65. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NZ_CP019758 (Borreliella burgdorferi strain B31_NRZ isolate B31 plasmid p_cp32-3, complete sequence) position: , mismatch: 7, identity: 0.863

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ctaaaatagacaatgttgaaaagaatttaaacctaaaaatggatggcttga	Protospacer
*******************************.********.***...**..

66. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NZ_CP015784 (Borrelia mayonii strain MN14-1420 plasmid cp32-3, complete sequence) position: , mismatch: 7, identity: 0.863

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ctaaaatagacaatgttgaaaagaatttaaacctaaaaatggatggcttga	Protospacer
*******************************.********.***...**..

67. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NZ_CP019926 (Borreliella burgdorferi strain PAbe plasmid p_lp56, complete sequence) position: , mismatch: 7, identity: 0.863

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ctaaaatagacaatgttgaaaagaatttaaacctaaaaatggatggcttga	Protospacer
*******************************.********.***...**..

68. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NC_017427 (Borreliella burgdorferi JD1 plasmid JD1 cp32-3, complete sequence) position: , mismatch: 7, identity: 0.863

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ctaaaatagacaatgttgaaaagaatttaaacctaaaaatggatggcttga	Protospacer
*******************************.********.***...**..

69. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NC_000956 (Borreliella burgdorferi B31 plasmid lp56, complete sequence) position: , mismatch: 7, identity: 0.863

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ctaaaatagacaatgttgaaaagaatttaaacctaaaaatggatggcttga	Protospacer
*******************************.********.***...**..

70. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NZ_CP019855 (Borreliella burgdorferi plasmid lp56, complete sequence) position: , mismatch: 7, identity: 0.863

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ctaaaatagacaatgttgaaaagaatttaaacctaaaaatggatggcttga	Protospacer
*******************************.********.***...**..

71. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NZ_CP019766 (Borreliella burgdorferi strain B31_NRZ isolate B31 plasmid p_lp56, complete sequence) position: , mismatch: 7, identity: 0.863

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ctaaaatagacaatgttgaaaagaatttaaacctaaaaatggatggcttga	Protospacer
*******************************.********.***...**..

72. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NC_015910 (Borreliella bissettii DN127 plasmid cp32-7, complete sequence) position: , mismatch: 7, identity: 0.863

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
ctaaaatagacaatgttgaaaagaatttaaacctaaaaatggatggcttta	Protospacer
*******************************.********.***...** .

73. spacer 1.2|22581|51|NZ_CP017204|PILER-CR matches to NC_015910 (Borreliella bissettii DN127 plasmid cp32-7, complete sequence) position: , mismatch: 8, identity: 0.843

ctaaaatagacaatgttgaaaagaatttaaatctaaaaatagataatttag	CRISPR spacer
aaaaattgattaatgtagaaaagaatttaaatctaaaaatagataatttag	Protospacer
  *** *.. .***** **********************************

74. spacer 1.1|22489|53|NZ_CP017204|PILER-CR matches to NC_015903 (Borreliella bissettii DN127 plasmid cp32-11, complete sequence) position: , mismatch: 12, identity: 0.774

---------caaaaggatatatctaatttagacattaagattgatactgtagaaaagaat	CRISPR spacer
gatactgtagaaaag---------aatttagacattaagattgatactgtagaaaagaat	Protospacer
          *****         ************************************

75. spacer 1.1|22489|53|NZ_CP017204|PILER-CR matches to NC_015903 (Borreliella bissettii DN127 plasmid cp32-11, complete sequence) position: , mismatch: 12, identity: 0.774

---------caaaaggatatatctaatttagacattaagattgatactgtagaaaagaat	CRISPR spacer
gatactgtagaaaag---------aatttagacattaagattgatactgtagaaaagaat	Protospacer
          *****         ************************************

76. spacer 1.1|22489|53|NZ_CP017204|PILER-CR matches to NZ_CP015798 (Borrelia mayonii strain MN14-1539 plasmid cp32-1, complete sequence) position: , mismatch: 12, identity: 0.774

-----caaaaggatatatctaatttagacattaagattgatactgtagaaaagaattt	CRISPR spacer
gatgccgtagagaag-----aatttagacattaagattgatactgtagagaagaattt	Protospacer
     *. *..** .     *****************************.********

Prophage detection

Region	Region Position	Protein_number	Hit_taxonomy	Key_proteins	Att_site	Prophage annotation

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

Overview of predicted results

Overview of the results

Cas Category Instructions

Results visualization

1. NZ_CP017206

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection

2. NZ_CP017201

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection

3. NZ_CP017204

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Click the colored protein region to show detailed information

Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection