CRISPRimmunity

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Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Self targeting spacer number	Target MGE spacer number	Prophage number	Anti-CRISPR protein number
NZ_CP025743	Brucella abortus strain CIIMS-NV-4 chromosome I, complete sequence	0 crisprs	csa3,WYL,DEDDh	0	0	2	0
NZ_CP025744	Brucella abortus strain CIIMS-NV-4 chromosome II, complete sequence	1 crisprs	csa3,cas3,DEDDh	0	0	0	0

Results visualization

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CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

545749 : 651907

Paenibacillus_phage(10.0%)

holin,capsid,integrase,tail,protease,transposase,portal,head

attL 644486:644500|attR 654378:654392

The bacterium proteins that are colored denote the protein is present at specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin' and 'tRNA')

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_109790517.1\|545749_546506_+\|transposase	IS5 family transposase	A0A0N9SJT9	Paenibacillus_phage	2.7e-16	41.0
WP_002968716.1\|546877_547996_+	glycosyltransferase family 4 protein	NA	NA	NA	NA
WP_077281774.1\|548753_548906_-	hypothetical protein	NA	NA	NA	NA
WP_126358176.1\|549140_549503_-	hypothetical protein	NA	NA	NA	NA
WP_094866392.1\|549665_550428_+\|transposase	IS5 family transposase	A0A0N9SHJ4	Paenibacillus_phage	1.3e-21	40.8
WP_002975117.1\|550325_550643_-	helix-turn-helix domain-containing protein	NA	NA	NA	NA
WP_002963688.1\|551253_552609_+	phosphomannomutase	NA	NA	NA	NA
WP_002963689.1\|552679_554104_+	mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase	A0A1V0SH58	Hokovirus	9.0e-53	30.8
WP_002963690.1\|554136_555309_+	AGE family epimerase/isomerase	NA	NA	NA	NA
WP_002969456.1\|555391_556501_+	glycosyltransferase	NA	NA	NA	NA
WP_109790518.1\|556849_557053_+	hypothetical protein	NA	NA	NA	NA
WP_002963692.1\|557192_558728_+	sugar ABC transporter ATP-binding protein	A0A2H4PQG7	Staphylococcus_phage	1.4e-14	25.3
WP_002963693.1\|558748_559750_+	ABC transporter permease	NA	NA	NA	NA
WP_002969455.1\|559828_560791_+	ABC transporter substrate-binding protein	NA	NA	NA	NA
WP_002963695.1\|560848_561901_+	sugar phosphate isomerase/epimerase	NA	NA	NA	NA
WP_002969454.1\|561908_563006_+	Gfo/Idh/MocA family oxidoreductase	NA	NA	NA	NA
WP_002966715.1\|563047_564355_-	xylose isomerase	NA	NA	NA	NA
WP_002966716.1\|564401_565853_-	xylulokinase	NA	NA	NA	NA
WP_002963699.1\|565885_566917_-	LacI family DNA-binding transcriptional regulator	NA	NA	NA	NA
WP_002971540.1\|567178_567697_-	helix-turn-helix transcriptional regulator	NA	NA	NA	NA
WP_002969452.1\|567603_568173_-	AraC family transcriptional regulator	NA	NA	NA	NA
WP_022576149.1\|568710_570174_-	betaine-aldehyde dehydrogenase	NA	NA	NA	NA
WP_002966717.1\|570325_572008_-\|holin	choline dehydrogenase	A0A1V0S9M4	Catovirus	7.9e-40	30.6
WP_002969060.1\|572010_572607_-	transcriptional regulator BetI	NA	NA	NA	NA
WP_002963705.1\|572797_573820_+	asparaginase	NA	NA	NA	NA
WP_002963706.1\|573864_574335_+	transcription elongation factor	NA	NA	NA	NA
WP_002963707.1\|574331_574721_-	type II toxin-antitoxin system death-on-curing family toxin	A0A1B3AYM0	Gordonia_phage	3.5e-07	44.6
WP_002963708.1\|574717_574948_-	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	NA	NA	NA	NA
WP_002963709.1\|575008_576001_-	magnesium transporter CorA family protein	NA	NA	NA	NA
WP_002963710.1\|576156_576834_+	FadR family transcriptional regulator	NA	NA	NA	NA
WP_002963712.1\|578165_578528_-	hypothetical protein	NA	NA	NA	NA
WP_002963713.1\|578863_580375_+	DUF853 domain-containing protein	A0A248XCZ8	Klebsiella_phage	1.4e-83	44.3
WP_002963714.1\|580564_581281_-	L,D-transpeptidase	NA	NA	NA	NA
WP_002971536.1\|581339_581582_+	hypothetical protein	NA	NA	NA	NA
WP_002963715.1\|581583_582237_-	haloacid dehalogenase type II	NA	NA	NA	NA
WP_002963716.1\|582459_583059_+	superoxide dismutase	Q56AR7	Bacillus_thuringiensis_phage	1.2e-38	42.6
WP_002963717.1\|583230_583566_-	hypothetical protein	NA	NA	NA	NA
WP_002963718.1\|583683_585792_-	S9 family peptidase	NA	NA	NA	NA
WP_002963720.1\|586364_586793_+	MucR family transcriptional regulator	A0A1P8VVG0	Erythrobacter_phage	1.0e-20	47.6
WP_002969861.1\|587000_587312_-	hypothetical protein	NA	NA	NA	NA
WP_002966719.1\|587492_587948_+	hypothetical protein	NA	NA	NA	NA
WP_002969449.1\|587944_588739_+	hypothetical protein	NA	NA	NA	NA
WP_002966720.1\|588849_590166_+	amidase	NA	NA	NA	NA
WP_002963725.1\|590206_590632_-	SufE family protein	NA	NA	NA	NA
WP_002963726.1\|590711_591161_-	hypothetical protein	NA	NA	NA	NA
WP_002969448.1\|591451_593281_+	HAMP domain-containing histidine kinase	W8CYF6	Bacillus_phage	4.7e-22	28.9
WP_002971535.1\|593255_594224_+	peptidoglycan-binding protein	NA	NA	NA	NA
WP_002963729.1\|594237_594585_+	DUF1491 family protein	NA	NA	NA	NA
WP_002969865.1\|594595_595672_-	DUF2336 domain-containing protein	NA	NA	NA	NA
WP_002963731.1\|595869_596412_-	hypothetical protein	NA	NA	NA	NA
WP_002963732.1\|596404_597016_-	DUF1214 domain-containing protein	NA	NA	NA	NA
WP_002969866.1\|597022_599197_-	penicillin-binding protein 1A	NA	NA	NA	NA
WP_002963734.1\|599536_600049_+	hypothetical protein	NA	NA	NA	NA
WP_002971534.1\|599987_601235_+	DNA-packaging protein	A0A1V0DY66	Dinoroseobacter_phage	1.9e-70	40.2
WP_002963736.1\|601266_602460_+\|portal	phage portal protein	W8ECU7	Geobacillus_phage	7.0e-67	40.0
WP_002963737.1\|602456_602834_+	hypothetical protein	NA	NA	NA	NA
WP_002963738.1\|602820_603489_+\|head,protease	HK97 family phage prohead protease	A0A2H4JAI0	uncultured_Caudovirales_phage	1.7e-30	41.1
WP_002966724.1\|603510_604785_+\|capsid	phage major capsid protein	Q8HAD2	Salmonella_phage	2.8e-82	43.6
WP_002963740.1\|604949_605516_+	hypothetical protein	NA	NA	NA	NA
WP_002963741.1\|605512_605851_+\|head	phage head closure protein	NA	NA	NA	NA
WP_006072374.1\|605973_606381_+	DUF3168 domain-containing protein	NA	NA	NA	NA
WP_002963743.1\|606421_606835_+\|tail	phage major tail protein, TP901-1 family	NA	NA	NA	NA
WP_002963744.1\|606831_607173_+	gene transfer agent family protein	NA	NA	NA	NA
WP_002963745.1\|607216_607387_+\|tail	phage tail assembly chaperone	NA	NA	NA	NA
WP_002970984.1\|607391_607937_+\|tail	phage tail tape measure protein	A0A0S2SYD9	Pseudomonas_phage	2.8e-07	46.4
WP_002963747.1\|607939_608572_+	TIGR02217 family protein	A0A0B5A2K3	Paracoccus_phage	2.9e-48	47.6
WP_002963749.1\|609439_609862_+	peptidase P60	NA	NA	NA	NA
WP_002969445.1\|613793_614000_+	hypothetical protein	NA	NA	NA	NA
WP_002971526.1\|614161_614458_+	hypothetical protein	NA	NA	NA	NA
WP_002963753.1\|614536_615226_+	response regulator transcription factor	NA	NA	NA	NA
WP_002969444.1\|615300_616611_+	hypothetical protein	NA	NA	NA	NA
WP_002971524.1\|616704_617157_+	hypothetical protein	NA	NA	NA	NA
WP_002969443.1\|617257_618397_+	c-type cytochrome biogenesis protein CcmI	NA	NA	NA	NA
WP_002963757.1\|618393_618891_+	cytochrome c maturation protein CcmE	NA	NA	NA	NA
WP_002963758.1\|618910_620902_+	heme lyase CcmF/NrfE family subunit	NA	NA	NA	NA
WP_002963759.1\|620898_621375_+	cytochrome c-type biogenesis protein CcmH	NA	NA	NA	NA
WP_002963760.1\|621516_623058_+\|protease	DegQ family serine endoprotease	A0A1B1IT49	uncultured_Mediterranean_phage	1.7e-28	29.8
WP_002963761.1\|623151_623859_+	response regulator transcription factor	NA	NA	NA	NA
WP_002966726.1\|623855_625262_+	HAMP domain-containing histidine kinase	NA	NA	NA	NA
WP_002963763.1\|625384_628336_+	bifunctional [glutamine synthetase] adenylyltransferase/[glutamine synthetase]-adenylyl-L-tyrosine phosphorylase	NA	NA	NA	NA
WP_002966727.1\|628380_630492_-	hypothetical protein	NA	NA	NA	NA
WP_002963765.1\|630762_633114_-	PAS domain-containing protein	A0A1V0SGX0	Hokovirus	6.3e-27	34.8
WP_002968720.1\|633504_636156_-	aminopeptidase N	NA	NA	NA	NA
WP_002963767.1\|636381_637341_+	DMT family transporter	NA	NA	NA	NA
WP_002970986.1\|637345_638602_-	MFS transporter	NA	NA	NA	NA
WP_002963769.1\|638763_639636_-	uracil-DNA glycosylase	NA	NA	NA	NA
WP_002963770.1\|640024_641716_+	electron transfer flavoprotein-ubiquinone oxidoreductase	NA	NA	NA	NA
WP_002969439.1\|641831_642800_-	AP endonuclease	NA	NA	NA	NA
WP_002963772.1\|642968_644471_-	AMP nucleosidase	NA	NA	NA	NA
644486:644500	attL	NA	NA	NA	NA
WP_002963775.1\|645037_645748_+	thiol:disulfide oxidoreductase	NA	NA	NA	NA
WP_002963776.1\|645806_646154_-	DUF2147 domain-containing protein	NA	NA	NA	NA
WP_002969438.1\|646150_646384_-	hypothetical protein	NA	NA	NA	NA
WP_002963778.1\|646653_646911_+	hypothetical protein	NA	NA	NA	NA
WP_002963779.1\|646970_647741_-	YitT family protein	NA	NA	NA	NA
WP_002963780.1\|648397_649048_+	hypothetical protein	NA	NA	NA	NA
WP_002963781.1\|649092_649467_-	DUF423 domain-containing protein	NA	NA	NA	NA
WP_002963782.1\|649484_650618_-	pyridoxal phosphate-dependent aminotransferase	A0A1X7QHI1	Faustovirus	9.1e-16	23.9
WP_002967515.1\|650980_651907_-\|integrase	site-specific integrase	A0A076YL28	Mesorhizobium_phage	2.9e-28	36.3
654378:654392	attR	NA	NA	NA	NA

DBSCAN-SWA_2

875887 : 887815

uncultured_Mediterranean_phage(90.0%)

tRNA

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_002968731.1\|875887_876739_+	segregation/condensation protein A	A0A1B1IVW1	uncultured_Mediterranean_phage	4.4e-31	33.5
WP_002964011.1\|876731_877457_+	SMC-Scp complex subunit ScpB	A0A1B1IVT7	uncultured_Mediterranean_phage	1.5e-43	45.7
WP_002964012.1\|877602_877821_+	twin-arginine translocase TatA/TatE family subunit	A0A1B1IVR9	uncultured_Mediterranean_phage	1.5e-07	65.1
WP_002969412.1\|877931_878513_+	twin-arginine translocase subunit TatB	NA	NA	NA	NA
WP_002964014.1\|878509_879334_+	twin-arginine translocase subunit TatC	A0A1B1IVR7	uncultured_Mediterranean_phage	9.8e-44	41.1
WP_002964015.1\|879410_880694_+\|tRNA	serine--tRNA ligase	A0A1B1IVT2	uncultured_Mediterranean_phage	1.2e-104	47.2
WP_002966786.1\|880842_881610_+	5'/3'-nucleotidase SurE	A0A1B1ITZ2	uncultured_Mediterranean_phage	1.0e-39	40.2
WP_002964017.1\|881606_882275_+	protein-L-isoaspartate(D-aspartate) O-methyltransferase	A0A1B1IU40	uncultured_Mediterranean_phage	4.2e-13	37.8
WP_002964018.1\|882419_882605_+	hypothetical protein	NA	NA	NA	NA
WP_002964019.1\|882653_883952_+	peptidoglycan DD-metalloendopeptidase family protein	Q8SBN9	Clostridium_phage	1.0e-18	41.1
WP_002964020.1\|884000_884867_-	ATP-binding protein	NA	NA	NA	NA
WP_002964021.1\|885027_885369_+	preprotein translocase subunit YajC	A0A1B1IVR5	uncultured_Mediterranean_phage	9.1e-12	40.2
WP_002969969.1\|885487_887815_+	protein translocase subunit SecDF	A0A1B1IVP7	uncultured_Mediterranean_phage	6.2e-51	43.5

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

2. NZ_CP025744

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CRISPR-Cas detection and classification

Crispr_ID: NZ_CP025744_1

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

NZ_CP025744_1

181991-182091

Orphan

Consensus_repeat	Method
ATGGACTTCTTGGAAGTCCATGCCGCCC	CRISPRCasFinder

1 spacers

The CRISPR arrays of NZ_CP025744_1

>merge|NZ_CP025744|1|181991-182091|CRISPRCasFinder
ATGGACTTCTTGGAAGTCCATGCCGCCCCCGCGCCGCTTTGCGGCGCAGGCATTTAGTTCATGGGCGAGCTTTATGGACTTCTTAGAAGTCCATGCCGCCC

>NZ_CP025744|1|1|181991-182091|CRISPRCasFinder
ATGGACTTCTTGGAAGTCCATGCCGCCC	CCGCGCCGCTTTGCGGCGCAGGCATTTAGTTCATGGGCGAGCTTT
ATGGACTTCTTAGAAGTCCATGCCGCCC

Protein	Signature genes	Signature genes Name	Protein_function
NZ_CP025744.1\|WP_002966386.1\|183803_184100_-\|co-chaperone-GroES	unknown	unknown	gnl\|CDD\|178988
NZ_CP025744.1\|WP_002966396.1\|173356_173974_+\|LysE-family-translocator	unknown	unknown	gnl\|CDD\|224199
NZ_CP025744.1\|WP_002967192.1\|190975_191536_+\|cytochrome-b	unknown	unknown	gnl\|CDD\|225580
NZ_CP025744.1\|WP_002966377.1\|193929_194493_+\|16S-rRNA-(guanine(966)-N(2))-methyltransferase-RsmD	unknown	unknown	gnl\|CDD\|223813
NZ_CP025744.1\|WP_002972153.1\|175601_176732_+\|ATP-phosphoribosyltransferase-regulatory-subunit	unknown	unknown	gnl\|CDD\|183413
NZ_CP025744.1\|WP_002966383.1\|185516_186506_+\|bifunctional-riboflavin-kinase/FAD-synthetase	unknown	unknown	gnl\|CDD\|235536
NZ_CP025744.1\|WP_002966379.1\|191589_192063_-\|nucleoside-deaminase	unknown	unknown	gnl\|CDD\|223663
NZ_CP025744.1\|WP_002966390.1\|179524_180916_-\|class-II-fumarate-hydratase	unknown	unknown	gnl\|CDD\|234779
NZ_CP025744.1\|WP_002966393.1\|176728_177424_+\|ATP-phosphoribosyltransferase	unknown	unknown	gnl\|CDD\|237438
NZ_CP025744.1\|WP_002969286.1\|194578_196123_+\|insulinase-family-protein	unknown	unknown	gnl\|CDD\|223685
NZ_CP025744.1\|WP_002966382.1\|186849_189768_+\|isoleucine--tRNA-ligase	unknown	unknown	gnl\|CDD\|237514
NZ_CP025744.1\|WP_002967189.1\|172581_173238_+\|DNA-3-methyladenine-glycosylase-I	unknown	unknown	gnl\|CDD\|377025
NZ_CP025744.1\|WP_002966378.1\|192203_193949_+\|pseudouridine-synthase	unknown	unknown	gnl\|CDD\|224108
NZ_CP025744.1\|WP_002968994.1\|170505_171333_-\|aldo/keto-reductase	unknown	unknown	gnl\|CDD\|381357
NZ_CP025744.1\|WP_002972151.1\|181065_181320_-\|hypothetical-protein	unknown	unknown	unknown
NZ_CP025744.1\|WP_002972147.1\|184580_185429_+\|TIGR01459-family-HAD-type-hydrolase	unknown	unknown	gnl\|CDD\|319827
NZ_CP025744.1\|WP_002971287.1\|189959_190631_+\|hypothetical-protein	unknown	unknown	unknown
NZ_CP025744.1\|WP_002966392.1\|177496_178735_-\|sugar-MFS-transporter	unknown	unknown	gnl\|CDD\|340952
NZ_CP025744.1\|WP_002968826.1\|174084_175593_+\|histidine--tRNA-ligase	unknown	unknown	gnl\|CDD\|223202
NZ_CP025744.1\|WP_002966391.1\|179020_179422_-\|DoxX-family-protein	unknown	unknown	gnl\|CDD\|225168

Protein	Function_ID	Function_description	E-value
NZ_CP025744.1\|WP_002966386.1\|183803_184100_-\|co-chaperone-GroES	gnl\|CDD\|178988	PRK00364, groES, co-chaperonin GroES; Reviewed.	5.80408e-54
NZ_CP025744.1\|WP_002966396.1\|173356_173974_+\|LysE-family-translocator	gnl\|CDD\|224199	COG1280, RhtB, Putative threonine efflux protein [Amino acid transport and metabolism].	9.51794e-45
NZ_CP025744.1\|WP_002966379.1\|191589_192063_-\|nucleoside-deaminase	gnl\|CDD\|223663	COG0590, CumB, Cytosine/adenosine deaminases [Nucleotide transport and metabolism / Translation, ribosomal structure and biogenesis].	5.96751e-65
NZ_CP025744.1\|WP_002966377.1\|193929_194493_+\|16S-rRNA-(guanine(966)-N(2))-methyltransferase-RsmD	gnl\|CDD\|223813	COG0742, COG0742, N6-adenine-specific methylase [DNA replication, recombination, and repair].	1.21532e-78
NZ_CP025744.1\|WP_002966392.1\|177496_178735_-\|sugar-MFS-transporter	gnl\|CDD\|340952	cd17394, MFS_FucP_like, Fucose permease and similar proteins of the Major Facilitator Superfamily of transporters. This subfamily is composed of L-fucose permease (also called L-fucose-proton symporter) and similar proteins such as glucose/galactose transporter and N-acetyl glucosamine transporter NagP. L-fucose permease facilitates the uptake of L-fucose across the boundary membrane with the concomitant transport of protons into the cell; it can also transport L-galactose and D-arabinose. Glucose/galactose transporter functions in the uptake of of glucose and galactose. The FucP-like subfamily belongs to the bacterial fucose permease, eukaryotic Major facilitator superfamily domain-containing protein 4 (FucP/MFSD4) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.	1.18971e-166
NZ_CP025744.1\|WP_002966383.1\|185516_186506_+\|bifunctional-riboflavin-kinase/FAD-synthetase	gnl\|CDD\|235536	PRK05627, PRK05627, bifunctional riboflavin kinase/FAD synthetase.	2.77791e-157
NZ_CP025744.1\|WP_002966390.1\|179524_180916_-\|class-II-fumarate-hydratase	gnl\|CDD\|234779	PRK00485, fumC, fumarate hydratase; Reviewed.	0
NZ_CP025744.1\|WP_002966393.1\|176728_177424_+\|ATP-phosphoribosyltransferase	gnl\|CDD\|237438	PRK13583, hisG, ATP phosphoribosyltransferase.	1.16784e-135
NZ_CP025744.1\|WP_002969286.1\|194578_196123_+\|insulinase-family-protein	gnl\|CDD\|223685	COG0612, PqqL, Predicted Zn-dependent peptidases [General function prediction only].	4.11164e-117
NZ_CP025744.1\|WP_002967192.1\|190975_191536_+\|cytochrome-b	gnl\|CDD\|225580	COG3038, CybB, Cytochrome B561 [Energy production and conversion].	7.89145e-52
NZ_CP025744.1\|WP_002967189.1\|172581_173238_+\|DNA-3-methyladenine-glycosylase-I	gnl\|CDD\|377025	pfam03352, Adenine_glyco, Methyladenine glycosylase. The DNA-3-methyladenine glycosylase I is constitutively expressed and is specific for the alkylated 3-methyladenine DNA.	3.88001e-109
NZ_CP025744.1\|WP_002966378.1\|192203_193949_+\|pseudouridine-synthase	gnl\|CDD\|224108	COG1187, RsuA, 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases [Translation, ribosomal structure and biogenesis].	6.08227e-83
NZ_CP025744.1\|WP_002968994.1\|170505_171333_-\|aldo/keto-reductase	gnl\|CDD\|381357	cd19131, AKR_AKR5C2, Escherichia coli 2,5-diketo-D-gluconic acid reductase A (DkgA/YqhE) and similar proteins. Escherichia coli DkgA/YqhE is a founding member of aldo-keto reductase family 5 member C2 (AKR5C2). DkgA/YqhE (EC 1.1.1.274), also called 2,5-DKG reductase A, or 2,5-DKGR A, or 25DKGR-A, or AKR5C, catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG). It is also capable of stereoselective -keto ester reductions on ethyl acetoacetate and other 2-substituted derivatives.	3.08102e-178
NZ_CP025744.1\|WP_002966382.1\|186849_189768_+\|isoleucine--tRNA-ligase	gnl\|CDD\|237514	PRK13804, ileS, isoleucyl-tRNA synthetase; Provisional.	0
NZ_CP025744.1\|WP_002972147.1\|184580_185429_+\|TIGR01459-family-HAD-type-hydrolase	gnl\|CDD\|319827	cd07525, HAD_like, uncharacterized family of the haloacid dehalogenase-like (HAD) hydrolase superfamily. The haloacid dehalogenase-like (HAD) hydrolases are a large superfamily of diverse enzymes that catalyze carbon or phosphoryl group transfer reactions on a range of substrates, using an active site aspartate in nucleophilic catalysis. Members include 2-L-haloalkanoic acid dehalogenase (C-Cl bond hydrolysis), azetidine hydrolase (C-N bond hydrolysis); phosphonoacetaldehyde hydrolase (C-P bond hydrolysis), phosphoserine phosphatase and phosphomannomutase (CO-P bond hydrolysis), P-type ATPases (PO-P bond hydrolysis) and many others. Members are found in all three kingdoms of life, and most genomes are predicted to contain multiple HAD-like proteins. Members possess a highly conserved alpha/beta core domain, and many also possess a small cap domain, the fold and function of which is variable. HAD hydrolases are sometimes referred to as belonging to the DDDD superfamily of phosphohydrolases.	2.51221e-90
NZ_CP025744.1\|WP_002972153.1\|175601_176732_+\|ATP-phosphoribosyltransferase-regulatory-subunit	gnl\|CDD\|183413	PRK12295, hisZ, ATP phosphoribosyltransferase regulatory subunit; Provisional.	2.67267e-157
NZ_CP025744.1\|WP_002968826.1\|174084_175593_+\|histidine--tRNA-ligase	gnl\|CDD\|223202	COG0124, HisS, Histidyl-tRNA synthetase [Translation, ribosomal structure and biogenesis].	2.87411e-159
NZ_CP025744.1\|WP_002966391.1\|179020_179422_-\|DoxX-family-protein	gnl\|CDD\|225168	COG2259, COG2259, Predicted membrane protein [Function unknown].	2.4232e-20

>NZ_CP025744.1|WP_002972151.1|181065_181320_-|hypothetical-protein
MRKELPIETAPKDGRKITVIWIDDDDQRNESIAQYRTLEQLRAGGGQWDEADTGWWTFTDNKTQRKIEPTAWIRETDDDESQED
>NZ_CP025744.1|WP_002966390.1|179524_180916_-|class-II-fumarate-hydratase
MAATRTETDTFGPIDVPADRYWGAQTQRSLQNFRIGGERMPLPLVHALGVVKRAAAETNIALGKLDPVLGQVIAVAASEVIEGKLDDHFPLVVWQTGSGTQSNMNANEVISNRAIELLGGEMGSKKPIHPNDHVNMSQSSNDSFPTAIHIATAVETVNRLYPALEHLTKALKVKEEAFKDIIKIGRTHTQDATPVTLGQEFSGYRAALEYARHRLEQSLADVFLLAQGGTAVGTGLNAPVGFDKGFAEAVSEITGLSFKTAPNKFEALASHGAVLNFHGSLNALAADLFKIANDIRFLGSGPRSGLGELSLPENEPGSSIMPGKVNPTQAEAMTMVATQVFGNQTAVTVAASQGHFELNVFKPVIAYNVLQSIRLLSDTMVSFADHCVEGIEPNTARIKELLERSLMLVTALAPAIGYDNAARIAKTAHKNGTTLREEALASGLVSEEDYDRLVRAERMIAPQ
>NZ_CP025744.1|WP_002966391.1|179020_179422_-|DoxX-family-protein
MSSITPKANGAATLIIRVFLSVLFIIAGYNKLTALDGTADYFASIGLPMPIVTAVVVGLIEFVGGLAILFGFKTRIAAVIVGLFTLGATLVAHMDFAEGMNLLIAQKNLAIAGGLFLLFLHGAGSLSIDARRG
>NZ_CP025744.1|WP_002966392.1|177496_178735_-|sugar-MFS-transporter
MATSIPTNNPLHTETSSQKNYGFALTSLTLLFFMWGFITCLNDILIPHLKNVFQLNYTQSMLIQFCFFGAYFIVSLPAGQLVKRISYKRGIVVGLIVAAIGCALFIPAASYRVYALFLGALFVLASGVTILQVAANPYVTILGKPETAASRLTLTQAFNSLGTTVAPVFGAVLILSAATDATVNAEADAVRFPYLLLALAFTVLAIIFAILKPPDVQEDEPALSDKKEGSAWQYRHLVLGAIGIFVYVGAEVSVGSFLVNFLSDPTVAGLSETDAAHHVAYFWGGAMVGRFIGSAAMRYIDDGKALAFNAFVAIILLFITVATTGHIAMWSVLAIGLFNSIMFPTIFSLALHGLGSHTSQGSGILCLAIVGGAIVPLIQGALADAIGIHLAFLMPIICYAYIAFYGLIGSKS
>NZ_CP025744.1|WP_002966393.1|176728_177424_+|ATP-phosphoribosyltransferase
MSVTLALPSKGRLKEKTLAVLEKAGYKVVLPDDDRNYRARVEGEDDLDILFLSASEIARELGYGSVDLGVTGEDLVRETLAHADERVAIEAQLGFGHADVVVAVPEVWRDVTTMADLDDVAADFRQRHGRRLRIATKYWRLTQQFFSQKHGIQVYRIVESLGATEGAPAAGSADMIVDITSTGSTLRANRLKVLEDGIILRSQACLVSARRSHTSRRVEEIAARIRAGLEI
>NZ_CP025744.1|WP_002972153.1|175601_176732_+|ATP-phosphoribosyltransferase-regulatory-subunit
MVGSRTSPIFNALRVELNAREAELVEIPLIQPADPFLDMAGEDLRRRIFLTENENGDSLCLRPEFTIPVCRNHIALNAATPKRYAYLGEVFRQRRDGAAEFLQAGIEDLGAADEAASDARSLADALSCVKAIAPDAPLEIVLGDQSVFAGMLKALGLPQGWRKKLLRSFGDAHSMDLALAELTGTQRRDPLPESLAVLVAEGDEIGLARMLEAEMLEAGISPGAGRTPVEIARRLIEKEDLAATHFPAAALDLLRQFLAIRVSLDMAAVTLRAFAADNALDLGAVLQKFEARADAIAQAGIEMKDIIYDASFGRPLDYYTGLVYEIRDASNRQDGVLAGGGRYDRLLTMLGACEAIPGVGFSIWLDRLQALAGEKQ
>NZ_CP025744.1|WP_002968826.1|174084_175593_+|histidine--tRNA-ligase
MADKADKMKARLPRGFVDRVPDDLRAAEKMMATIREVYDLYGFEPVETPLVEYTDALGKFLPDQDRPNEGVFSFQDDDEQWLSLRYDLTAPLARYVAENFETLPKPYRSYRNGWVFRNEKPGPGRFRQFMQFDADTVGAPNVSADAEMCMMMADTLERLGIQRGDYAIRVNNRKVLDGVLDAIGLEGEGNAAKRLNVLRAIDKLDKFGPEGVRLLLGKGRLDESGDFTKGAQLPEAAIEKVLAFTAAGGADGAQTIANLQAVVAGNAKGEEGVQELADMQALFFAGGYEGRVKIDPSVVRGLEYYTGPVFEAELLFDVTNEDGQKVVFGSVGGGGRYDGLVSRFRGEPVPATGFSIGVSRLMTALKNLGKLDVSDTVGPVVVLVMDKDTQNLGRYQKMVSDLRKAGIRAEMYVGGSGMKAQMKYADRRAAPCVVIQGSQEREAGEVQIKDLVEGKRLSAEIEDNVTWLESRPAQITVREDGLVDAVREILDAQARDRAEQSK
>NZ_CP025744.1|WP_002966396.1|173356_173974_+|LysE-family-translocator
MDLMSLVAFAGILVVAAGTPGPKVGALVARVISRGHKGVFPFMFGLWLGDAVWLSLAVWGLSALAHTFQTAFLVLKYAGVAYLIYLSWKMWFAPVDEEADENTIPRQGEGKRLFLSALGVTLGNPKIMVFYLAILPTVVDITHVSLTGWVELLLVMFMVLAAVDTAWVVLAAQARKFLRSPRMMRLANRTSAGMMAGAAVAIAAR
>NZ_CP025744.1|WP_002967189.1|172581_173238_+|DNA-3-methyladenine-glycosylase-I
MSDMAEARAGLVVGADGRTRCFWPGELPDYLAYHDNEWGVPVADDFRLFEKICLEGFQSGLSWLTILRKRENFRAAFDGFNFHRIATYDESDVERLLADKGIVRHRGKIVSVINNAGRAIELIEEKGSLAAYFWSFEPGAENRPAVVDYPTLVANPITATSTRISKDLKKRGWSFVGPTTVYAFMQAMGLVNDHIEGCHCRATIEKLRQNFKRPGKKL
>NZ_CP025744.1|WP_002968994.1|170505_171333_-|aldo/keto-reductase
MTVPTVKLNDGNHIPQLGYGVWQISNDEAVSAVSEALKAGYRHIDTATIYGNEEGVGKAINGSGIARADIFLTTKLWNSDQGYESTLKAFDTSLKKLGTDYVDLYLIHWPMPSKDLFMETWRAFIKLKEEGRVKSIGVSNFRTADLERLIKESGVTPVLNQIELHPQFQQDELRLFHGKHDIATEAWSPLGQGKLLEDPTLKSIAEKHAKSVAQIILRWHIETGNIVIPKSITPARIKENFDIFDFTLNGTDHDAITKLDMADGRIGPNPSVFSA
>NZ_CP025744.1|WP_002966386.1|183803_184100_-|co-chaperone-GroES
MADIKFRPLHDRVVVRRVESEAKTAGGIIIPDTAKEKPQEGEVVAAGAGARDEAGKLVPLDVKAGDRVLFGKWSGTEVKIGGEDLLIMKESDILGIVG
>NZ_CP025744.1|WP_002972147.1|184580_185429_+|TIGR01459-family-HAD-type-hydrolase
MILPERLDDLTDRYDAIFCDVWGVVHNGETSFAPAIAALQRARAKGVTIILVTNSPRPHPGVVAQMSLLGVPEDAYDRVVTSGDVTRDLIAEGPRRIFHIGCERELAIYDGLDVELVEEFEAAGVVCTGLYDDEVETPEDYRELLQRLRARNLPFICANPDIMVERGPRLIWCAGALAREYGQLGGRTLIAGKPHRPIYEAALRAVESIRGGSVDKSRILGIGDGVLTDVKGAADFGLDVLYISGGVHAADYAVNGDLDMAKMEAFLEKHGHRPIASLHALV
>NZ_CP025744.1|WP_002966383.1|185516_186506_+|bifunctional-riboflavin-kinase/FAD-synthetase
MKKSSFQRLTGTEGLPPALHDCVVAIGNFDGVHRGHQAVLERALELAERESRPAVVLTFEPHPRSFFKQDQPVDRLTDAAEKAEILRLMGFDAVMEQPFTAEFSQRSAEDFVQHILVEKLRASRVVTGYDFHFGKGRRGTPEFLCEAGKKAGFSVTLVDAFTDEGGMLVSSTRIRNLLSEGEVAEAAGLLGYRYRICGEVIHGKKLGRTLGFPTANMALAPQTSLKHGIYAVRFRLDDAMLYDGVASFGRRPTVDDEGAPLLETFLFDFSGDLYGKTAAVSFFGFLRGEVKFDGLDPLIDQMKQDEAQARALLADVRPISGLDRMLSFN
>NZ_CP025744.1|WP_002966382.1|186849_189768_+|isoleucine--tRNA-ligase
MTDTTKIDYSKTLYLPQTEFPMRAGLPQREPLFVQRWEEMNLYKKLREQAKDRPLYVLHDGPPYANGNIHIGHALNKILKDVITRSFQMRGYNSNYVPGWDCHGLPIEWKIEEKYRAAGKNKDEVPINEFRKECREFASNWIKVQTEEFKRLAILGDFENPYTTMNFHAEARIAGELLKFAASGQLYRGSKPVMWSVVERTALAEAEVEYHDIESDMIWVKFPVAGEVATENDLSGSAVVIWTTTPWTIPGNRAVSYSSRIEYGLFEITEAENDFGPRPGERLVFADKLVEECCAKAKLQFKRLRSVSAEELGKIVLDHPLKGFGGGYEFVVPMLDGDHVTDDAGTGFVHTAPSHGREDFEAWMDNARQLEARGIDPNIPFPVGDDGFYTKDAPGFGPDREGGPARVIDDNGKKGDANKVVIEQLIAADKLFARGRLKHSYPHSWRSKKPVIFRNTPQWFVYMDKNLGDGTTLRSRALKAIDETRFVPAAGQTRLRSMIEGRPDWVLSRQRAWGVPICVFVDEEGNILQDDAVNKRIMDAFEKEGADAWFADGARERFLGARAGEGWTQVRDILDVWFDSGSTHTFTLEDRPDLKWPADVYLEGSDQHRGWFHSSLLESCGTRGRAPYNAVVTHGFTMDEHGKKMSKSLGNTVTPQDVIKESGADILRLWVMTTDYWEDQRLGKSIIQTNIDAYRKLRNTIRWMLGTLAHDEGENVAYADLPELERLMLHRLTELDELVRSGYDTFDFKRIARALVDFMNVELSAFYFDIRKDALYCDAPSSIRRKAALQTVREIFVRLTTWLAPMLPFTMEEAWLDRYPQSVSIHAEQFRPTPAEWRDDVLAEKWRKVRAVRRVVTGALELERADKRIGSSLEAAPVVYIADKSLSDSLEGLDFAEICITSGISVSDAAAPEGAFTLGDVKGVAVVPERAKGEKCARSWRYTTDVGADPEFPEVSARDAAALRELQALGKL
>NZ_CP025744.1|WP_002971287.1|189959_190631_+|hypothetical-protein
MKIEGRVLVLMTALAGLSLSGCVSSPTYGTDKSAGAQLLDDVSNMASFGQGKKKERIDYKPRPDLVRPAPGDKGYLPAPQQSLASSSDPNWPESPEQRRKRVRDEITAHRDDPNYVSPVLQDAPVADNIRTALPRGNSRREDYASRTPTSDPAKVAALKAARAQQNQGSATTRRYLSEPPLAYRQPASTAAVGELGPDEAQKERERKKAASGSRGWRDYLPWN
>NZ_CP025744.1|WP_002967192.1|190975_191536_+|cytochrome-b
MSSKTYIWDDRSGYGLVSRGFHWLMALLFLWQFASAILRVFAKDTPIYSFFWSAHFQLGFALLVLALLRGVWGLMNIHRRPHKDGAIGKLAVLGHLAIYVLMITIPSLALLRAYGSGRGFSFLGMPIFAKTDIQNAMLTAPANALHGPLGWALLAAIVGHVAMALVYHFALRDDTLRYMTGHGTPN
>NZ_CP025744.1|WP_002966379.1|191589_192063_-|nucleoside-deaminase
MRKAASITKANSATPMDIALEEAHAAGERGEVPIGAVIVRDGEIIARAGNRTREFNDVTAHAEILTIRQAGEMLGSERLIDCDLYVTLEPCAMCAAAISFARIRRLYYGASDPKGGGIEHGGRFYTQPTCHHAPEIYPGFCEADARKILKDFFREKR
>NZ_CP025744.1|WP_002966378.1|192203_193949_+|pseudouridine-synthase
MMAQGDAVTLLKGLYQMTTEDDNKGGKPPHGRKGHSDRPGRPDWDSRPRREGGDRDKPRKFGSRGAPVEEETSERIAKRLARAGIASRREAETMIAAGRIAVNGKVLESPAVNVKRTDIITVDGKPLRQTERTRLWLYHKPAGLVTTNRDPEGRPTVFEALPPEMPRVLSVGRLDINTEGLLLLTNDGGLSRVLELPSTGWLRRYRVRAHGKVTQQQLDELKNGIAVDGVFYGSVEAELERVQGANVWISIGLREGKNREVKNILGALGLTVGRLIRVSFGPFQLGDLEEGAVREIKGRTLRDQLGDKLIEESGADFDAPVLNEFSNAAVQGRTRREMEEGELPQEGERRRAPREREWISSTGERRRGREEKTEKVEPRRRGNANVWMAPGARPKGEKEAAKPARVEHQQPHRSAKFSGDLQMRPRRFSEEERGGEESGERRYKRPEGRFEREGGRPDNREKYEKHEGAGKERRPGKRERAELRNAEGQTTERGKGGFEGRKPGKPGGPRSEGRFGGADRDTRSGPRGSGPRGAGDGPRRGHDGKPGGKPGGKPTGRPGGKPGTKPGGGGRSGGGGADRRR
>NZ_CP025744.1|WP_002966377.1|193929_194493_+|16S-rRNA-(guanine(966)-N(2))-methyltransferase-RsmD
MRIVGGKFRGRALVTPSTNAIRPTTDRTRESLFNILAHSFPDKVEGVRVLDLFAGTGALGLEALSRGARYATFVEESAEGRGILRQNIEALGLQGHTKILRRDACQLGIVGTMEPFDLVFADPPYGRRMGEKAFLSALQGGWLNPDALLVLEEDAQAALELDDRFAVREERNYGGTVIRLIELKAAS
>NZ_CP025744.1|WP_002969286.1|194578_196123_+|insulinase-family-protein
MNTPHSGCLLLSTVLGFALALPLAGGAVRAEMQPAAPAETQPAPAAPAAPVAPAALPEIFRLDGVSNFTLPNGMQVVVIPDHRAPVVTQMVWYHVGAADEAPGVSGIAHFLEHLMFKGTKNHPAGEFSARIASIGGQENAFTSYDYTAYFQRVSPEALEMVMDFESDRMENLVLDEEAVKTEREVILEERRMRIDSNPGAMLMENTDAVLFYNHPYRKPVIGWQQEMEKLSLKNAIDFYNQYYTPNNATLVIAGDVTPERVRELAMKTWANVHKRAEVLLRERPQEPAKHAARVVTLHDERVSTPSFRISWLVPSYANEKRFANAKPGDAPALDLLSEILGGSQLSQLYQQLIVKQGIAAETGASYDGDALDDGTFSVYGVPRNGASLGDVEKAVAAQVDRIIRDGVTQAELDQARNRFLKAVIFARDSQTGMARIYGSALSVGQTVDDIQKWPDLIKSVTVDQIKDVARRYLVKDQAVTSYLLPPDTEAESARKKPNAPANDASASGAGGAIQ

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Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region	Region Position	Protein_number	Hit_taxonomy	Key_proteins	Att_site	Prophage annotation

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

Overview of predicted results

Overview of the results

Cas Category Instructions

Results visualization

1. NZ_CP025743

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CRISPR-Cas detection and classification

Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection

2. NZ_CP025744

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CRISPR-Cas detection and classification

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Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection