CRISPRimmunity

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Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Self targeting spacer number	Target MGE spacer number	Prophage number	Anti-CRISPR protein number
NZ_CP026648	Vibrio cholerae O1 biovar El Tor strain HC1037 chromosome II, complete sequence	0 crisprs	csa3,cas3	0	0	0	0
NZ_CP026647	Vibrio cholerae O1 biovar El Tor strain HC1037 chromosome I, complete sequence	0 crisprs	csa3,DEDDh,cas3,WYL,DinG,csx1,RT	0	0	5	0

Results visualization

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CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

51695 : 105858

Bacillus_phage(25.0%)

integrase,tRNA,protease,transposase

attL 38993:39008|attR 114933:114948

The bacterium proteins that are colored denote the protein is present at specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin' and 'tRNA')

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
38993:39008	attL	NA	NA	NA	NA
WP_000146472.1\|51695_52883_+\|tRNA	tyrosine--tRNA ligase	NA	NA	NA	NA
WP_001890498.1\|52950_54414_-	serine-type D-Ala-D-Ala carboxypeptidase	NA	NA	NA	NA
WP_001883445.1\|54493_55546_-	porin OmpU	NA	NA	NA	NA
WP_001883448.1\|56073_56547_+	transcription elongation factor GreA	NA	NA	NA	NA
WP_001054291.1\|56623_56920_-	ribosome assembly RNA-binding protein YhbY	NA	NA	NA	NA
WP_000043220.1\|57039_57669_+	23S rRNA (uridine(2552)-2'-O)-methyltransferase RlmE	NA	NA	NA	NA
WP_001883452.1\|57769_59725_+\|protease	ATP-dependent zinc metalloprotease FtsH	A0A0P0BXI2	Ostreococcus_lucimarinus_virus	2.4e-120	44.4
WP_000913082.1\|59791_60628_+	dihydropteroate synthase	A0A0B5J4J5	Pandoravirus	3.2e-18	32.6
WP_001281065.1\|60653_61994_+	phosphoglucosamine mutase	NA	NA	NA	NA
WP_000495557.1\|62250_62586_+	preprotein translocase subunit SecG	NA	NA	NA	NA
WP_000147148.1\|63016_63472_+	ribosome maturation factor RimP	NA	NA	NA	NA
WP_000031601.1\|63490_64978_+	transcription termination/antitermination protein NusA	NA	NA	NA	NA
WP_000192207.1\|65002_67699_+	translation initiation factor IF-2	NA	NA	NA	NA
WP_001123656.1\|67800_68199_+	30S ribosome-binding factor RbfA	NA	NA	NA	NA
WP_000124044.1\|68198_69137_+\|tRNA	tRNA pseudouridine(55) synthase TruB	NA	NA	NA	NA
WP_000055654.1\|69278_69548_+	30S ribosomal protein S15	NA	NA	NA	NA
WP_000462070.1\|69910_72040_+	polyribonucleotide nucleotidyltransferase	NA	NA	NA	NA
WP_001883460.1\|72155_73067_+	lipoprotein NlpI	NA	NA	NA	NA
WP_001883461.1\|73153_73573_+	MarR family transcriptional regulator	NA	NA	NA	NA
WP_000981941.1\|73619_74957_+	sodium-coupled multidrug efflux MATE transporter VcrM	NA	NA	NA	NA
WP_000872504.1\|75009_75888_-	U32 family peptidase	NA	NA	NA	NA
WP_000421327.1\|75899_76913_-	U32 family peptidase	NA	NA	NA	NA
WP_001111411.1\|77020_79075_-	bifunctional diguanylate cyclase/phosphodiesterase	NA	NA	NA	NA
WP_000932672.1\|79307_79835_+	SCP2 domain-containing protein	NA	NA	NA	NA
WP_001883466.1\|79770_80322_+	N-acetyltransferase	A0A1P8CWJ6	Bacillus_phage	1.5e-19	32.2
WP_000953212.1\|80432_80840_+	DNA polymerase III subunit psi	NA	NA	NA	NA
WP_001883468.1\|80800_81286_+	ribosomal protein S18-alanine N-acetyltransferase	NA	NA	NA	NA
WP_000160066.1\|81274_83332_-	EAL domain-containing protein	A0A127AWB9	Bacillus_phage	6.9e-14	34.3
WP_001910883.1\|83535_83634_+	hypothetical protein	NA	NA	NA	NA
WP_000127615.1\|84072_84261_+	hypothetical protein	NA	NA	NA	NA
WP_000211147.1\|84406_86425_+	DNA mismatch repair protein	NA	NA	NA	NA
WP_001218618.1\|86554_87796_-\|integrase	integrase family protein	A0A291AWU1	Escherichia_phage	1.3e-76	39.8
WP_001995600.1\|87797_88067_-	hypothetical protein	NA	NA	NA	NA
WP_000497807.1\|88069_89044_-	ParM/StbA family protein	NA	NA	NA	NA
WP_000348524.1\|89508_89952_+	hypothetical protein	NA	NA	NA	NA
WP_000985631.1\|92125_95104_+\|transposase	Tn3 family transposase	NA	NA	NA	NA
WP_001120888.1\|95521_97015_+\|transposase	IS91 family transposase	NA	NA	NA	NA
WP_001447541.1\|97045_97930_+	DUF3363 domain-containing protein	NA	NA	NA	NA
WP_000214119.1\|98146_99361_+	chloramphenicol/florfenicol efflux MFS transporter FloR	S4TR35	Salmonella_phage	4.2e-19	28.7
WP_001255015.1\|99388_99694_+	LysR family transcriptional regulator	NA	NA	NA	NA
WP_001120891.1\|99805_100345_+\|transposase	IS91 family transposase	NA	NA	NA	NA
WP_000480968.1\|100316_101153_-	aminoglycoside O-phosphotransferase APH(6)-Id	NA	NA	NA	NA
WP_001082316.1\|101152_101956_-	aminoglycoside O-phosphotransferase APH(3'')-Ib	Q75ZG1	Hepacivirus	3.8e-24	34.3
WP_001043260.1\|102016_102832_-	sulfonamide-resistant dihydropteroate synthase Sul2	NA	NA	NA	NA
WP_001122813.1\|104646_105858_-\|transposase	IS256 family transposase	A0A218MNI5	uncultured_virus	4.2e-51	51.4
114933:114948	attR	NA	NA	NA	NA

DBSCAN-SWA_2

262444 : 269637

Faustovirus(16.67%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_000775253.1\|262444_263659_+	IscS subfamily cysteine desulfurase	A0A0H3TPH2	Faustovirus	1.1e-32	30.5
WP_000331703.1\|263695_264079_+	Fe-S cluster assembly scaffold IscU	A0A218MKD1	uncultured_virus	5.4e-53	78.2
WP_000301571.1\|264139_264463_+	iron-sulfur cluster assembly protein IscA	A0A2H4N7N5	Lake_Baikal_phage	3.0e-25	50.5
WP_001105747.1\|264511_265027_+	co-chaperone HscB	NA	NA	NA	NA
WP_001196560.1\|265050_266901_+	Fe-S protein assembly chaperone HscA	A0A0N9QXN8	Chrysochromulina_ericina_virus	5.3e-106	41.1
WP_001124187.1\|266914_267253_+	ISC system 2Fe-2S type ferredoxin	NA	NA	NA	NA
WP_000872176.1\|267301_267496_+	Fe-S cluster assembly protein IscX	NA	NA	NA	NA
WP_000107237.1\|267715_269005_+	aminopeptidase PepB	Q6GYZ8	Mycoplasma_phage	1.7e-34	34.3
WP_001162850.1\|269208_269637_+	nucleoside-diphosphate kinase	D2E8E1	Anguillid_herpesvirus	1.0e-20	38.4

DBSCAN-SWA_3

1024987 : 1041651

Vibrio_phage(44.44%)

transposase

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_000593523.1\|1024987_1025362_-	cholera enterotoxin binding subunit CtxB	B9V4E7	Vibrio_virus	1.0e-64	100.0
WP_001881225.1\|1025358_1026135_-	cholera enterotoxin catalytic subunit CtxA	A0A142I6Y0	Vibrio_phage	1.3e-151	100.0
WP_000021616.1\|1026240_1027440_-	zonula occludens toxin ZOT	A0A142I6Z1	Vibrio_phage	5.3e-240	100.0
WP_000979342.1\|1027436_1027730_-	accessory cholera enterotoxin	A0A0F6YNQ7	Vibrio_phage	3.7e-46	100.0
WP_001881237.1\|1027726_1029010_-	hypothetical protein	A0A142I701	Vibrio_phage	9.1e-222	100.0
WP_000493022.1\|1029020_1029269_-	colonization factor	A0A0N7F140	Vibrio_phage	8.8e-33	100.0
WP_001888513.1\|1029404_1029785_-	hypothetical protein	A0A0N9HJ67	Vibrio_phage	4.0e-69	100.0
WP_000743997.1\|1029762_1030842_-	hypothetical protein	U5TQR3	Satellite_phage	2.2e-213	100.0
WP_000693566.1\|1030967_1031306_+	helix-turn-helix transcriptional regulator	U5TNA1	Satellite_phage	7.3e-54	100.0
WP_000053920.1\|1031817_1032042_-	RstC protein	U5TMI6	Satellite_phage	1.1e-34	100.0
WP_010895442.1\|1032135_1032510_-	hypothetical protein	B9V3G5	Vibrio_virus	1.2e-68	100.0
WP_000743997.1\|1032487_1033567_-	hypothetical protein	U5TQR3	Satellite_phage	2.2e-213	100.0
WP_000693566.1\|1033692_1034031_+	helix-turn-helix transcriptional regulator	U5TNA1	Satellite_phage	7.3e-54	100.0
WP_000170634.1\|1034520_1035105_-	DNA-binding protein	E3U9I9	Vibrio_phage	6.4e-114	100.0
WP_001161489.1\|1035097_1035265_-	hypothetical protein	E3U9J0	Vibrio_phage	2.3e-13	100.0
WP_001052673.1\|1035342_1036035_-	helix-turn-helix transcriptional regulator	NA	NA	NA	NA
WP_001881265.1\|1036164_1036449_-	hypothetical protein	NA	NA	NA	NA
WP_002003155.1\|1036453_1038019_-	replication protein	A7BJY2	Enterobacteria_phage	3.9e-57	32.3
WP_001265358.1\|1038180_1038747_+	helix-turn-helix transcriptional regulator	NA	NA	NA	NA
WP_086026889.1\|1039055_1040277_+\|transposase	IS3-like element ISVch4 family transposase	Q716C2	Shigella_phage	1.5e-117	65.1
WP_001122813.1\|1040439_1041651_-\|transposase	IS256 family transposase	A0A218MNI5	uncultured_virus	4.2e-51	51.4

DBSCAN-SWA_4

1876678 : 1883295

Staphylococcus_phage(66.67%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_000864130.1\|1876678_1877149_-	6,7-dimethyl-8-ribityllumazine synthase	A0A2H4PQS3	Staphylococcus_phage	6.8e-34	48.7
WP_001122865.1\|1877413_1878523_-	bifunctional 3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP cyclohydrolase II	A0A2H4PQS2	Staphylococcus_phage	6.7e-64	39.8
WP_000493874.1\|1878563_1879217_-	riboflavin synthase	A0A2H4PQS5	Staphylococcus_phage	1.2e-31	38.2
WP_001131994.1\|1879221_1880325_-	bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD	A0A2H4PQS8	Staphylococcus_phage	1.3e-43	33.1
WP_000543544.1\|1880350_1880800_-	transcriptional regulator NrdR	NA	NA	NA	NA
WP_001890340.1\|1880899_1882153_-	glutamate-5-semialdehyde dehydrogenase	A0A1X9I5D4	Streptococcus_phage	5.0e-100	49.5
WP_000210573.1\|1882161_1883295_-	glutamate 5-kinase	A0A1X9I5D0	Streptococcus_phage	1.4e-64	40.4

DBSCAN-SWA_5

2957091 : 2964354

uncultured_Mediterranean_phage(33.33%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_001883363.1\|2957091_2957883_+	5'/3'-nucleotidase SurE	A0A1B1ITZ2	uncultured_Mediterranean_phage	8.4e-69	51.6
WP_000002982.1\|2957875_2958502_+	protein-L-isoaspartate(D-aspartate) O-methyltransferase	A0A1B1IU40	uncultured_Mediterranean_phage	2.0e-36	52.1
WP_000177568.1\|2958501_2959437_+	murein hydrolase activator NlpD	D7RWE0	Brochothrix_phage	7.8e-05	34.7
WP_000116737.1\|2959510_2960518_+	RNA polymerase sigma factor RpoS	F4YCU2	Synechococcus_phage	6.0e-35	34.2
WP_001894770.1\|2960610_2963199_-	DNA mismatch repair protein MutS	A0A1V0SC35	Catovirus	2.2e-33	23.0
WP_001279365.1\|2963466_2964354_-	cysteine synthase CysM	A0A1X9I5K7	Streptococcus_phage	4.1e-56	41.1

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage