CRISPRimmunity

Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Self targeting spacer number	Target MGE spacer number	Prophage number	Anti-CRISPR protein number
NZ_CP032479	Helicobacter pylori strain 21-F-EK1 chromosome, complete genome	3 crisprs	DEDDh	0	3	0	0

1. NZ_CP032479

CRISPR-Cas detection and classification

Crispr_ID: NZ_CP032479_1

• CRISPR_ID: NZ_CP032479_1
• CRISPR_location: 350863-351090
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 3 spacers

Consensus repeat of NZ_CP032479_1

Consensus_repeat	Method
CGCTAACAATGAAATAAACACCAAAGAAAC	CRISPRCasFinder

spacers of NZ_CP032479_1

>1.1|350893|36|NZ_CP032479|CRISPRCasFinder
CCAAAGCCTTGAAGCGATTACACTTGCTAAAACAAC
>1.2|350959|36|NZ_CP032479|CRISPRCasFinder
GCAAGTGCTAAATAACATTAACGAAGCGAAAGAAAG
>1.3|351025|36|NZ_CP032479|CRISPRCasFinder
CGAAAGCCTTGAAGCGATCACAGAAGCGAAAACGAA

CRISPR arrays and Neighbor proteins around NZ_CP032479_1

CRISPR arrays

The CRISPR arrays of NZ_CP032479_1

>merge|NZ_CP032479|1|350863-351090|CRISPRCasFinder
CGCTAACAATGAAATAAACACCAAAGAAACCCAAAGCCTTGAAGCGATTACACTTGCTAAAACAACCGCTAACAATGAAATAAACACCAAAGAAACGCAAGTGCTAAATAACATTAACGAAGCGAAAGAAAGCACTACAACGCAAATAAACACCAACAAAACCGAAAGCCTTGAAGCGATCACAGAAGCGAAAACGAACGCTAACAATCAAATTACCGCTAACCAAAC

>NZ_CP032479|1|1|350863-351090|CRISPRCasFinder
CGCTAACAATGAAATAAACACCAAAGAAAC	CCAAAGCCTTGAAGCGATTACACTTGCTAAAACAAC
CGCTAACAATGAAATAAACACCAAAGAAAC	GCAAGTGCTAAATAACATTAACGAAGCGAAAGAAAG
CACTACAACGCAAATAAACACCAACAAAAC	CGAAAGCCTTGAAGCGATCACAGAAGCGAAAACGAA
CGCTAACAATCAAATTACCGCTAACCAAAC

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
NZ_CP032479.1|WP_001179287.1|339601_340159_+|peptidylprolyl-isomerase	unknown	unknown	gnl|CDD|223976
NZ_CP032479.1|WP_000260887.1|341895_342330_+|hypothetical-protein	unknown	unknown	unknown
NZ_CP032479.1|WP_139542785.1|347497_350323_+|DUF3519-domain-containing-protein	unknown	unknown	gnl|CDD|376185
NZ_CP032479.1|WP_000238147.1|355762_356707_-|pyruvate-ferredoxin-oxidoreductase-subunit-beta	unknown	unknown	gnl|CDD|239471
NZ_CP032479.1|WP_139542790.1|359880_361188_-|hypothetical-protein	unknown	unknown	unknown
NZ_CP032479.1|WP_139542787.1|353431_354265_-|outer-membrane-beta-barrel-protein	unknown	unknown	gnl|CDD|280099
NZ_CP032479.1|WP_000656174.1|357952_358345_-|4Fe-4S-binding-protein	unknown	unknown	gnl|CDD|236596
NZ_CP032479.1|WP_139542788.1|354330_355653_-|adenylosuccinate-lyase	unknown	unknown	gnl|CDD|181437
NZ_CP032479.1|WP_139543558.1|351444_353421_+|excinuclease-ABC-subunit-B	unknown	unknown	gnl|CDD|235395
NZ_CP032479.1|WP_139542791.1|361373_362570_+|glycosyltransferase-family-8-protein	unknown	unknown	gnl|CDD|224359
NZ_CP032479.1|WP_139543557.1|340825_341764_+|DNA-(cytosine-5-)-methyltransferase	unknown	unknown	gnl|CDD|273211
NZ_CP032479.1|WP_001874956.1|359211_359874_+|outer-membrane-protein	unknown	unknown	gnl|CDD|280099
NZ_CP032479.1|WP_139542782.1|342331_344152_+|flagellar-hook-associated-protein-FlgK	unknown	unknown	gnl|CDD|236270
NZ_CP032479.1|WP_000486469.1|358360_358921_-|pyruvate-flavodoxin-oxidoreductase-subunit-gamma	unknown	unknown	gnl|CDD|180284
NZ_CP032479.1|WP_139542789.1|356719_357943_-|2-oxoacid:ferredoxin-oxidoreductase-subunit-alpha	unknown	unknown	gnl|CDD|181999
NZ_CP032479.1|WP_001088955.1|340190_340397_+|hypothetical-protein	unknown	unknown	unknown
NZ_CP032479.1|WP_139542784.1|347328_347529_-|hypothetical-protein	unknown	unknown	unknown
NZ_CP032479.1|WP_139542783.1|344351_346055_+|gamma-glutamyltransferase	unknown	unknown	gnl|CDD|129176
NZ_CP032479.1|WP_108521961.1|346320_347091_+|HP1117-family-Sel1-like-repeat-protein	unknown	unknown	gnl|CDD|223861
NZ_CP032479.1|WP_001022310.1|340433_340664_+|flagellar-biosynthesis-anti-sigma-factor-FlgM	unknown	unknown	gnl|CDD|377300

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
NZ_CP032479.1|WP_001179287.1|339601_340159_+|peptidylprolyl-isomerase	gnl|CDD|223976	COG1047, SlpA, FKBP-type peptidyl-prolyl cis-trans isomerases 2 [Posttranslational modification, protein turnover, chaperones].	2.07879e-52
NZ_CP032479.1|WP_139542788.1|354330_355653_-|adenylosuccinate-lyase	gnl|CDD|181437	PRK08470, PRK08470, adenylosuccinate lyase; Provisional.	0
NZ_CP032479.1|WP_139542785.1|347497_350323_+|DUF3519-domain-containing-protein	gnl|CDD|376185	pfam18763, ddrB-ParB, ddrB-like ParB superfamily domain. A member of the ParB/sulfiredoxin superfamily of proteins found in polyvalent proteins prototyped by the version in the phage P1 ddRB protein. These proteins are predicted to function as nucleases.	1.74059e-32
NZ_CP032479.1|WP_000238147.1|355762_356707_-|pyruvate-ferredoxin-oxidoreductase-subunit-beta	gnl|CDD|239471	cd03376, TPP_PFOR_porB_like, Thiamine pyrophosphate (TPP family), PFOR porB-like subfamily, TPP-binding module; composed of proteins similar to the beta subunit (porB) of the Helicobacter pylori four-subunit pyruvate ferredoxin oxidoreductase (PFOR), which are also found in archaea and some hyperthermophilic bacteria. PFOR catalyzes the oxidative decarboxylation of pyruvate to form acetyl-CoA, a crucial step in many metabolic pathways. Archaea, anaerobic bacteria and eukaryotes that lack mitochondria (and therefore pyruvate dehydrogenase) use PFOR to oxidatively decarboxylate pyruvate, with ferredoxin or flavodoxin as the electron acceptor. The 36-kDa porB subunit contains the binding sites for the cofactors, TPP and a divalent metal cation, which are required for activity.	3.64339e-136
NZ_CP032479.1|WP_139542787.1|353431_354265_-|outer-membrane-beta-barrel-protein	gnl|CDD|280099	pfam01856, HP_OMP, Helicobacter outer membrane protein. This family seems confined to Helicobacter. It is predicted to be an outer membrane protein based on its pattern of alternating hydrophobic amino acids similar to porins.	3.99884e-34
NZ_CP032479.1|WP_000656174.1|357952_358345_-|4Fe-4S-binding-protein	gnl|CDD|236596	PRK09625, porD, pyruvate flavodoxin oxidoreductase subunit delta; Reviewed.	5.72644e-84
NZ_CP032479.1|WP_139543558.1|351444_353421_+|excinuclease-ABC-subunit-B	gnl|CDD|235395	PRK05298, PRK05298, excinuclease ABC subunit UvrB.	0
NZ_CP032479.1|WP_139542791.1|361373_362570_+|glycosyltransferase-family-8-protein	gnl|CDD|224359	COG1442, RfaJ, Lipopolysaccharide biosynthesis proteins, LPS:glycosyltransferases [Cell envelope biogenesis, outer membrane].	2.03418e-82
NZ_CP032479.1|WP_001874956.1|359211_359874_+|outer-membrane-protein	gnl|CDD|280099	pfam01856, HP_OMP, Helicobacter outer membrane protein. This family seems confined to Helicobacter. It is predicted to be an outer membrane protein based on its pattern of alternating hydrophobic amino acids similar to porins.	3.83763e-36
NZ_CP032479.1|WP_139542782.1|342331_344152_+|flagellar-hook-associated-protein-FlgK	gnl|CDD|236270	PRK08471, flgK, flagellar hook-associated protein FlgK; Validated.	0
NZ_CP032479.1|WP_139542789.1|356719_357943_-|2-oxoacid:ferredoxin-oxidoreductase-subunit-alpha	gnl|CDD|181999	PRK09622, porA, 2-oxoacid:ferredoxin oxidoreductase subunit alpha.	0
NZ_CP032479.1|WP_000486469.1|358360_358921_-|pyruvate-flavodoxin-oxidoreductase-subunit-gamma	gnl|CDD|180284	PRK05844, PRK05844, pyruvate flavodoxin oxidoreductase subunit gamma; Validated.	2.19254e-128
NZ_CP032479.1|WP_139542783.1|344351_346055_+|gamma-glutamyltransferase	gnl|CDD|129176	TIGR00066, Contains:_Gamma-glutamyltranspeptidase_large_chain, gamma-glutamyltranspeptidase. Also called gamma-glutamyltranspeptidase (ggt). Some members of this family have antibiotic synthesis or resistance activities. In the case of a cephalosporin acylase from Pseudomonas sp., the enzyme was shown to retain some gamma-glutamyltranspeptidase activity. Other, more distantly related proteins have ggt-related activities and score below the trusted cutoff. [Biosynthesis of cofactors, prosthetic groups, and carriers, Glutathione and analogs].	0
NZ_CP032479.1|WP_139543557.1|340825_341764_+|DNA-(cytosine-5-)-methyltransferase	gnl|CDD|273211	TIGR00675, Modification_methylase, DNA-methyltransferase (dcm). All proteins in this family for which functions are known are DNA-cytosine methyltransferases. This family is based on the phylogenomic analysis of JA Eisen (1999, Ph.D. Thesis, Stanford University). [DNA metabolism, DNA replication, recombination, and repair].	3.10734e-119
NZ_CP032479.1|WP_001022310.1|340433_340664_+|flagellar-biosynthesis-anti-sigma-factor-FlgM	gnl|CDD|377300	pfam04316, FlgM, Anti-sigma-28 factor, FlgM. FlgM binds and inhibits the activity of the transcription factor sigma 28. Inhibition of sigma 28 prevents the expression of genes from flagellar transcriptional class 3, which include genes for the filament and chemotaxis. Correctly assembled basal body-hook structures export FlgM, relieving inhibition of sigma 28 and allowing expression of class 3 genes. NMR studies show that free FlgM is mostly unfolded, which may facilitate its export. The C terminal half of FlgM adopts a tertiary structure when it binds to sigma 28. All mutations in FlgM that prevent sigma 28 inhibition affect the C-terminal domain and is the region thought to constitute the binding domain. A minimal binding domain has been identified between Glu 64 and Arg 88 in Salmonella typhimurium. The N-terminal portion remains unstructured and may be necessary for recognition by the export machinery.	8.32524e-05
NZ_CP032479.1|WP_108521961.1|346320_347091_+|HP1117-family-Sel1-like-repeat-protein	gnl|CDD|223861	COG0790, COG0790, FOG: TPR repeat, SEL1 subfamily [General function prediction only].	3.36315e-38

Protein sequences

>NZ_CP032479.1|WP_139542785.1|347497_350323_+|DUF3519-domain-containing-protein
MKLPKALNEATAGAALKYHIKRALERSHSISEFSKNLELSAKNAKFSNNTLKIIEELNNGVKQASEEIKEATKTSNLVKSIREQDTRPFEVIEDKEAFFKDLNKNLKDNATPLPKGMSVEEFKQSLESVENKDRLLEHLETRDNSQDRLKFLSLIEPILREPHIEIFIKDKDSGIAKKEYIKAFKDENKTRLYMLITQDNDTILRTFIPKLNERYMRSHVRDADIIHSFIQQNRTAKSDNALSDVVVYGDNPTPKPLNSQEDLLKTSENLNETTKDAKNLSPLEQANAKKLAKLQREQEQSEQEFLKAKEQETKRKVALKKKLEHERGNAGNIESQTKIEVGEDIPTNTQEQLPKSRVRLNEREIYDLDYAIVKAKDLKPSFTTGGTQKRTDMNEEQIKSIAQNFDPKKIFGSGGFEDLPIILHDGQVIAGNHRIAGMLNFTPKSRFAYEKAIKEYYHIDLKPDDLLVRIPNKRLNNTEINNLAASSNQGRFNSESDHAIAVLSHYEAKLKELDKKLDADSIYSLKNIVAKNLNFDKATHPNVGDSNLALLMFNMPRTKTQGIELLNRWQKEFSNDIKSYEKVKKMFVDNAGSFHNLIHDMNFPNVSLNAYLSDIMDRSFANLKNYQSTSESLKDLSEKFYKTSSLEMFEKSEQGSSDISEILGGAIARFARFDDPSKALFEALRSDNIKKGLKEFKIADVTKDMFNADRKEFKDIDIYDFTHYLLMANREPNENNPTLKRLIEAVKDMQKESEKGIKEKLEIPSEWGHNYSEFKGDGLGAINKLLETKKGFVAGAFYKEGLGDIDLVWGTPKTKDSNGYGLAHILERRISNEMKKGLNETEAKEYAINIVKSIPEVLEKGTKGTDHLGRVFVDYGNKRVGLNNEWKNEKLENHWVISSYELYDTEKQALRSTPQAITKEKAFNSLNSVEPNPTTKKLNKE
>NZ_CP032479.1|WP_139542784.1|347328_347529_-|hypothetical-protein
MASFKALGNFTPKMRSGALRYSVPSVNLIHWYFNAPLKVVAKPWLKFFVVAGFRLKAILASFLKSL
>NZ_CP032479.1|WP_108521961.1|346320_347091_+|HP1117-family-Sel1-like-repeat-protein
MGYASKLALKICLASLCLFSVLGAEHLEQKRNFIYKGEEAYNNKEYERAASFYKSAIKNGEPLAYVLLGIMYENGRGVPKDYKKAAEYFQKAVDNDIPRGYNNLGVMYKEGRGVPKDEKKAVEYFRIATEKGYANAYINLGIMYMEGRGVPSNYVKATECFRKAMHKGNVEAYILLGDIYYSGNDQLGIEPDKDKAIVYYKMAADMSSSRAYEGLAESYQYGLGVEKDKKKAEEYMQKACDFDIDKNCKKKNTSSR
>NZ_CP032479.1|WP_139542783.1|344351_346055_+|gamma-glutamyltransferase
MRRSFLKTIGLGVIALSLGLLSPLSAASYPPIKNTKVGLALSSHPLASEIGQKVLEDGGNAIDAAVAIGFALAVVHPAAGNIGGGGFAVIHLANGENVALDFREKAPLKATKNMFLDKQGNVVPKLSEDGYLAAGVPGTVAGMEAMLKKYGTKKLSQLIDPAIKLAENGYAISQRQAETLKEARERFLKYSSSKKYFFKKGHLDYQEGDLFVQKDLAKTLNQIKTLGAKGFYQGQVAELIEKDMKKNGGIITKEDLASYNVKWRKPVMGSYRGYKIISMSPPSSGGTHLIQILNVMENADLSALGYGASKNIHIAAEAMRQAYADRSVYMGDSDFVSVPVDKLINKAYAKKIFDTIQPDTVTPSSQIKPGMGQLHEGSNTTHYSVADRWGNAVSVTYTINASYGSAASIDGAGFLLNNEMDDFSIKPGNPNLYGLVGGDANAIEANKRPLSSMSPTIVLKNNKVFLVVGSPGGSRIITTVLQVISNVIDYNMNISEAVSAPRFHMQWLPDELRIEKFGMPADVKDNLTKMGYQIVTKPVMGDVNAIQVLPKTKGSVFYGSTDPRKEF
>NZ_CP032479.1|WP_139542782.1|342331_344152_+|flagellar-hook-associated-protein-FlgK
MGGILSSLNTSYTGLQAHQSMVDVTGNNISNASDEFYSRQRVIAKPQAAYMYGTKNVNMGVDIEAIERVHDEFVFARYTKANYENTYYDTEFSHLKEASAYFPDIDEASLFTDLQDYFNSWKELSKNAKDSAQKQALAQKTEALTHNIKDTRERLTTLQHKASEELKSVIKEVNSLGSQIAEINKRIKEVENNKSLKHANELRDKRDELEFHLRELLGGNVFKSSIKTHSLTDKDSADFDESYNLNIGHGFNIIDGSIFHPLVVKESENKGGLNQVYFQSDDFKLTNITDKLNQGKVGALLNVYNDGSNGTLKGKLQDYIDLLDSFARGLIESTNAIYAQSASHHIEGEPVEFNSDEAFKDTNYNIKNGSFDLIAYNTDGKEIARKTIAITPITTMNDIIQAINANTDDNQDNNTENDFDDYFTASFNNETKKFVIQPKNASQGLFVSMKDDGTNFMGALKLNPFFQGDDASNISLNKEYKKEPTTIRPWLAPINGNFDVANMMQQLQYDSVDFYNDKFDIKPMKISEFYQFLTGKINTDAEKSGRILDTKKSMLETIKKEQLSISQVSVDEEMVNLIKFQSGYAANAKVITAIDRMIDTLLGIKQ
>NZ_CP032479.1|WP_000260887.1|341895_342330_+|hypothetical-protein
MVVLHSHLENALNQLKELIDLTECDIRDIKLAKHTEIFERNHQKQLAIQAFEKEKANIDAQMLSLKNQFPNKEMSELLDEKTSDFLNQMRESLLVLKEKNLIYSRMAFAVSEFYSSLIQQIIPHDTCDYKGSRHVGSHFLRVQA
>NZ_CP032479.1|WP_139543557.1|340825_341764_+|DNA-(cytosine-5-)-methyltransferase
MDFCSGIGGGRLGLERCHLKCVGHAEINDEALRTYELFFKDTHNFGDLMRINPNDLPDFDVLVSGFPCQAFSINGKRKGLEDERGTIIYGLIRILKVKQPKCFLLENVKGLIHHKQQETFKTIIKALQEAGYTTYYQILNSADFQLAQKRERLYIVGFRKDLKHPFHFPLGLANDYYFKDFLDAGNECYLDVSNAAFQRYLRNPYNHNRVFLENILTLENAVLDTRQSDLRLYFNVFPTLRTSRHGLFYTQKGKIKKLNAIESLLLQGFPRGLIAKIKNNPNFKESHLLSQAGNAMSVNVIAAIAKQMLKAI
>NZ_CP032479.1|WP_001022310.1|340433_340664_+|flagellar-biosynthesis-anti-sigma-factor-FlgM
MNIKLKDFTMINAVSSLAPVQSLGNYKRVEKNEKVENSEAALDRVAEIKKAIENNQYKINLHETSHKMAQDLLGIS
>NZ_CP032479.1|WP_001088955.1|340190_340397_+|hypothetical-protein
MNSSNLKNWLFPTICFFLFCYILIFLMFFMFKSLQSQSFGSVAETGKKPITTTKKFGKELQKQISKIH
>NZ_CP032479.1|WP_001179287.1|339601_340159_+|peptidylprolyl-isomerase
MQNHDLESIKQAALIEYEVREQGSSEVLDGNISKEPLEFIIGANQIIAGLEKAVLKAQIGEWEEVVIAPEEAYGVYESSYLQEVPRDQFEGIELEKGMSVFGQTEDNQTIQATIKDFSNTHVMVDYNHPLAGKTLAFRFKVLGFREVSEEEILASHHGGGTGCCGGHGGHGGKKGGGCGCSCSHG
>NZ_CP032479.1|WP_139543558.1|351444_353421_+|excinuclease-ABC-subunit-B
MPLFDLKSPYPPAGDQPQAIEALTKSLKNNNHYQTLVGVTGSGKTYTMANIIAQTNKPALIMSHNKTLCAQLYSEFKAFFPHNRVEYFISHFDYYQPESYIPRRDLFIEKDSSINDDLERLRLSATTSLLGYDDVIVIASVSANYGLGNPEEYLKVMEKIKVGEKRVYKSFLLKLVEMGYSRNEVVFDRGSFRATGECVDIFPAYNDAEFIRIEFFGDEIERIAVFDALERNEIRRLDSVMLYAASQFAVGSERLNLAIKSIEDELALRLKFFKEQDKMLEYNRLKQRTEHDLEMISATGVCKGIENYARHFTGKAPNETPFCLFDYLGIFEREFLVIVDESHVSLPQFGGMYAGDMSRKSVLVEYGFRLPSALDNRPLKFDEFIHKNCQFLFVSATPNKLELELSKKNVAEQIIRPTGLLDPKFEVRDSDKQVQDLFDEIKLVVARDERVLITTLTKKMAEELCKYYAEWGLKVRYMHSEIDAIERNHIIRSLRLKEFDILIGINLLREGLDLPEVSLVAIMDADKEGFLRSETSLIQTMGRAARNANGKVLLYAKKITQSMQKAFEITSYRRAKQEEFNKIHNITPKTVTRALEEELKLRDDEAKIAKALKKDKIPKSEREKIIKELDKKMRECAKNLDFEEAMRLRDEIAQLRTL
>NZ_CP032479.1|WP_139542787.1|353431_354265_-|outer-membrane-beta-barrel-protein
MKRALYLILGLSYALHADSFKDVLTKGDYTFFNKKVVSPIKRYADRSAFYLGLGYQLGSIQHNYSNLNLSQQFNKSQIIFSDGLSPVFKNSYVSNGLGVQVGYKWVGKHEETKWFGFRWGLFYDLSVSLYGLKESQSVIISTYGSYMDLLLNAYNGDKFFAGFNLGIAFAGVYDKVSDASLYQALLLDTFGGKVNPNGFQFLVDLGVRLGNKRNQFGFGIKIPTYYFNHYYSMNNISNNSEDVLKVLRFLEYGINSLLYQVDFRRNYSVYFNYTYSF
>NZ_CP032479.1|WP_139542788.1|354330_355653_-|adenylosuccinate-lyase
MLERYANEEMKTLWNEQTKFETYLEVEKAVVRAWNKLGQIQDSDCEKICAKAAFNLERIKEIEKTTKHDLIAFTTCVAESLGEESRFFHYGITSSDCIDTAMALLMTKSLKLIQKGVKNLYETLKNRALEHKDTLMVGRSHGVFGEPITFGLVLALFADEIKRHLKALDLTMEFISVGAISGAMGNFAHAPLELEELACEFLGLKTANISNQVIQRDRYARLACDLALLASSCEKIAVNIRHLQRSEVYEVEEAFSTGQKGSSAMPHKRNPILSENITGLCRVIRSFTTPMLENVALWHERDMSHSSVERFALPDLFITSDFMLSRLNSVIENLVVYPKNMLKNLALSGGLVFSQRVLLELPKKGLSREESYSIVQENAMKIWEVLQQGAFKNADENLFLNALLNDERLKKYLSEDEIKACFDYSYYTKNMGAIFKRVFG
>NZ_CP032479.1|WP_000238147.1|355762_356707_-|pyruvate-ferredoxin-oxidoreductase-subunit-beta
MVKEVKTLKGFSQSAEKFQGSHLLCPGCGHGIIVREVLNAVDGPIVLGNSTGCLEVCSAVYPHTSWDVPWIHIGFENGSTAISGVEAMYKALVNKGRYQGQKPKFVAFGGDGASYDIGFQFISGCMERGHDMTYICLDNENYANTGGQRSGSTPLGASTSTTPAGSVSFGKKEKKKDIVNIMASHGVPYVAQLSPNKWKDMNKKIKTALDTEGPCFINALSPCTTEWKFESNKTIELADMAVDSLMFPLFEIFNGRELKITYRPRNIIPVRDYLAAQKRFKHLFKKENEHIIEELQKDVNERWEYLQRREEAKV
>NZ_CP032479.1|WP_139542789.1|356719_357943_-|2-oxoacid:ferredoxin-oxidoreductase-subunit-alpha
MARSIELQEIEVWDGNTASSNALRQAQIDVIAAYPITPSTPIVQNYGSFKDNGYVDGEFVLVESEHAAMSACVGAAAAGGRVSTATSSQGLALMVEVLYQASGMRLPIVLNLVNRALAAPLNIHGDHSDMYLSRDSGWISLCTCNPQEAYDFTLMAFRIAEHQKVRVPAIVNQDGFLCSHTVQNVRPLSDAVAYQFVGEYQTKHSLLDFDKPVSYGAQAEEEWHYEHKAQLHHAIMSASSVIEEVFNDFAKLTGRQYHLTKTFQLEDAEIAIFALGTTYESAIVAAKEMRKKGIKAGVATIHSLRPFPYERLGQDLKNLKALAILDKSSPAGAMGAMFNEVTSAVYQTQGTKHPVVSNYIYGLGERDMTIAHLCEIFEEINKDALKGTLTHPTQQFVGLHGPKMSFF
>NZ_CP032479.1|WP_000656174.1|357952_358345_-|4Fe-4S-binding-protein
MKDWNEFEMGAVLFPFEKNAQSEMEKHNDERHYTEQSYFTTSVAHWRVAKPVHNNNICINCFNCWVYCPDAAILSREGKLKGVDYSHCKGCGVCVDVCPTNPKSLWMFEEQIEPATALTQWPQKQEKKKS
>NZ_CP032479.1|WP_000486469.1|358360_358921_-|pyruvate-flavodoxin-oxidoreductase-subunit-gamma
MFQIRWHARAGQGAITGAKGLADVISKTGKEVQAFASYGSAKRGAAMMAYNRVDDEPILNHERFMQPDYVLVIDPGLVFIENIFANEKEDTTYIITSYLSKEELFEKKPELKTRKVFLVDCLKISMETLKRPIPNTPMLGALMKVSGMLEIEAFKEAFKKVLGKKLTQEVIDANMLAIQRAYEEVQ
>NZ_CP032479.1|WP_001874956.1|359211_359874_+|outer-membrane-protein
MNKTTVKILMGMALLSSLQAAEAELDEKSKKPKFADRNTFYLGVGYQLSAINTSFSTESVDKSYFMTGNGFGVVLGGKFVAKTQAVEHVGFRYGLFYDQTFSSHKSYISTYGLEFSGLWDAFNSPKMFLGLEFGLGIAGATYMPGGAMHGIIAQNLGKENSLFQLLVKVGFRFGFFHNEITFGLKFPVIPNKRTEIVDGLSATTLWHRLPVAYFNYIYNF
>NZ_CP032479.1|WP_139542790.1|359880_361188_-|hypothetical-protein
MIFLKKSLCALLFSGFLTPPLVKAASFVYDLKFMSFNFNLVAPNNNPYWNSLTKMQGRLMPQIGVQLDKRQALMFGAWFIQNLHTHYSYFPYSWGVTMYYQYIGKNLRFFLGIVPRSYQIGHYPLSAFKKLFWFIDPTFRGGAFQFKPAYDPNRWWNGWFEGVVDWYGGRNWNNQPKKKNYDFDQFLYFVSSEFQFLKGYLGLGGQLVVFHNASHHSMGDNYPYGGNSYLKPGDITPQWPNGYPYFSQKNNPQGGEIGKYSNPTILDRVYYHAYLKADFKNLMPYMDNIFMTFGTQSSQTHYCVRYASECKNARFYNSFGGEFYAQAQYKGFGIFNRYYFSNKPQMHFYATYGQSLYTGLPWYRAPNFDMIGLYYVYKNKWISVRADAFFNFLGGGDGYHLYGKGGKWITTYQQFLTLTIDTRELIDFVKSKTSK
>NZ_CP032479.1|WP_139542791.1|361373_362570_+|glycosyltransferase-family-8-protein
MTSDSSHSFKEQDFHIPIAFAFDKNYLIPAGACLYSLLESIAKANKKIRYTLHALVVGLNEEDKAKLNQITEPFKEFAVLEVKDIEPFLDTIPNPFDEDFTKRFSKMVLVKYFLADLFPKYSKMVWSDVDVIFCNEFSADFLSIKENDENYFYGVLEVEKHHMMEGFLFCNLDYQRKKNFTLRMHEILKGNETKGELDFTKWCWPNMKALGIEYCVFPYYYTIRDFSNAYLNENYKKTILEARENPTIIHYDAWWGAVKPWDYPFGLKADLWLNALAKTPFMSDYTKKMHTNESFYTTKMAEQHYFSSVKSSKEIVFKAPYLFFKSYLFVVFKERKIHSRVFELTCNLVKKFFNKLIYFGFFMPKALAKRVVSKILRILGLHGIVKKILIQLKLLRKG

Crispr_ID: NZ_CP032479_2

• CRISPR_ID: NZ_CP032479_2
• CRISPR_location: 548323-548399
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 1 spacers

Consensus repeat of NZ_CP032479_2

Consensus_repeat	Method
TCAATCCGCTTTTTAATCTTTAA	CRISPRCasFinder

spacers of NZ_CP032479_2

>2.1|548346|31|NZ_CP032479|CRISPRCasFinder
ATTTTTGATCTTTAATTTTTTCACTGATTTT

CRISPR arrays and Neighbor proteins around NZ_CP032479_2

CRISPR arrays

The CRISPR arrays of NZ_CP032479_2

>merge|NZ_CP032479|2|548323-548399|CRISPRCasFinder
TCAATCCGCTTTTTAATCTTTAAATTTTTGATCTTTAATTTTTTCACTGATTTTTCAATCCGCTTTTCAATCTTTAA

>NZ_CP032479|2|2|548323-548399|CRISPRCasFinder
TCAATCCGCTTTTTAATCTTTAA	ATTTTTGATCTTTAATTTTTTCACTGATTTT
TCAATCCGCTTTTCAATCTTTAA

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
NZ_CP032479.1|WP_139542897.1|548472_548784_+|hypothetical-protein	unknown	unknown	unknown
NZ_CP032479.1|WP_139542901.1|552474_556416_-|autotransporter-outer-membrane-beta-barrel-domain-containing-protein	unknown	unknown	gnl|CDD|111576
NZ_CP032479.1|WP_139542895.1|543394_544507_+|hydrogenase-formation-protein-HypD	unknown	unknown	gnl|CDD|237892
NZ_CP032479.1|WP_000973846.1|542190_542319_+|hypothetical-protein	unknown	unknown	unknown
NZ_CP032479.1|WP_139542904.1|559588_561433_+|DUF342-domain-containing-protein	unknown	unknown	unknown
NZ_CP032479.1|WP_139542890.1|534445_536263_-|flagellar-hook-basal-body-complex-protein	unknown	unknown	gnl|CDD|235620
NZ_CP032479.1|WP_139542894.1|541880_542180_+|cupin-domain-containing-protein	unknown	unknown	gnl|CDD|380358
NZ_CP032479.1|WP_139542900.1|551636_552404_+|ATP-binding-cassette-domain-containing-protein	unknown	unknown	gnl|CDD|224045
NZ_CP032479.1|WP_000003606.1|542427_543156_+|hydrogenase-nickel-incorporation-protein-HypB	unknown	unknown	gnl|CDD|272891
NZ_CP032479.1|WP_000921449.1|550656_551637_+|iron-ABC-transporter-permease	unknown	unknown	gnl|CDD|376439
NZ_CP032479.1|WP_100969100.1|543155_543395_+|HypC/HybG/HupF-family-hydrogenase-formation-chaperone	unknown	unknown	gnl|CDD|223375
NZ_CP032479.1|WP_139542899.1|549815_550664_+|SDR-family-NAD(P)-dependent-oxidoreductase	unknown	unknown	gnl|CDD|187632
NZ_CP032479.1|WP_139542902.1|556638_558036_-|cysteine--tRNA-ligase	unknown	unknown	gnl|CDD|234705
NZ_CP032479.1|WP_139542892.1|537200_538766_-|flagellar-hook-length-control-protein-FliK	unknown	unknown	gnl|CDD|376734
NZ_CP032479.1|WP_139542898.1|548770_549043_+|type-II-toxin-antitoxin-system-mRNA-interferase-toxin,-HP0892-family	unknown	unknown	gnl|CDD|225583
NZ_CP032479.1|WP_139542903.1|558036_559497_-|murein-biosynthesis-integral-membrane-protein-MurJ	unknown	unknown	gnl|CDD|367301
NZ_CP032479.1|WP_139542896.1|545494_547699_+|outer-membrane-beta-barrel-protein	unknown	unknown	gnl|CDD|375731
NZ_CP032479.1|WP_139542891.1|536259_537150_-|flagellar-hook-assembly-protein-FlgD	unknown	unknown	gnl|CDD|235621
NZ_CP032479.1|WP_033592703.1|549151_549676_-|acyl-CoA-thioesterase	unknown	unknown	gnl|CDD|224523
NZ_CP032479.1|WP_139542893.1|539956_540565_+|phosphate-acetyltransferase	unknown	unknown	gnl|CDD|235537

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
NZ_CP032479.1|WP_139542901.1|552474_556416_-|autotransporter-outer-membrane-beta-barrel-domain-containing-protein	gnl|CDD|111576	pfam02691, VacA, Vacuolating cyotoxin. This family consists of Vacuolating cyotoxin proteins form Proteobacteria. These proteins are an important virulence determinate in H. pylori and induce cytoplasmic vacuolation in a variety of mammalian cell lines.	0
NZ_CP032479.1|WP_139542895.1|543394_544507_+|hydrogenase-formation-protein-HypD	gnl|CDD|237892	PRK15062, PRK15062, hydrogenase isoenzymes formation protein HypD; Provisional.	0
NZ_CP032479.1|WP_139542890.1|534445_536263_-|flagellar-hook-basal-body-complex-protein	gnl|CDD|235620	PRK05841, flgE, flagellar hook protein FlgE; Validated.	0
NZ_CP032479.1|WP_139542894.1|541880_542180_+|cupin-domain-containing-protein	gnl|CDD|380358	cd02230, cupin_HP0902-like, Helicobacter pylori HP0902 and related proteins, cupin domain. This family includes prokaryotic and archaeal proteins homologous to HP0902, a functionally uncharacterized protein from Helicobacter pylori and Spy1581, a protein of unknown function from Streptococcus pyogenes. These proteins demonstrate all-beta cupin folds that cannot bind metal ions due to the absence of a metal-binding histidine that is conserved in many metallo-cupins. HP0902 is able to bind bacterial endotoxin lipopolysaccharides (LPS) through its surface-exposed loops, where metal-binding sites are usually found in other metallo-cupins, and thus may have a putative role in H. pylori pathogenicity.	5.22766e-19
NZ_CP032479.1|WP_139542900.1|551636_552404_+|ATP-binding-cassette-domain-containing-protein	gnl|CDD|224045	COG1120, FepC, ABC-type cobalamin/Fe3+-siderophores transport systems, ATPase components [Inorganic ion transport and metabolism / Coenzyme metabolism].	3.48118e-99
NZ_CP032479.1|WP_000003606.1|542427_543156_+|hydrogenase-nickel-incorporation-protein-HypB	gnl|CDD|272891	TIGR00073, nickel_incorporation_protein_HypB, hydrogenase accessory protein HypB. A GTP hydrolase for assembly of nickel metallocenter of hydrogenase. A similar protein, ureG, is an accessory protein for urease, which also uses nickel. hits scoring 75 and above are safe as orthologs. [SS 1/05/04 I changed the role_ID and process GO from protein folding to to protein modification, since a protein folding role has not been established, but HypB is implicated in insertion of nickel into the large subunit of NiFe hydrogenases.] [Protein fate, Protein modification and repair].	1.82685e-108
NZ_CP032479.1|WP_000921449.1|550656_551637_+|iron-ABC-transporter-permease	gnl|CDD|376439	pfam01032, FecCD, FecCD transport family. This is a sub-family of bacterial binding protein-dependent transport systems family. This Pfam entry contains the inner components of this multicomponent transport system.	1.38217e-66
NZ_CP032479.1|WP_100969100.1|543155_543395_+|HypC/HybG/HupF-family-hydrogenase-formation-chaperone	gnl|CDD|223375	COG0298, HypC, Hydrogenase maturation factor [Posttranslational modification, protein turnover, chaperones].	1.46363e-31
NZ_CP032479.1|WP_139542899.1|549815_550664_+|SDR-family-NAD(P)-dependent-oxidoreductase	gnl|CDD|187632	cd05374, 17beta-HSD-like_SDR_c, 17beta hydroxysteroid dehydrogenase-like, classical (c) SDRs. 17beta-hydroxysteroid dehydrogenases are a group of isozymes that catalyze activation and inactivation of estrogen and androgens. SDRs are a functionally diverse family of oxidoreductases that have a single domain with a structurally conserved Rossmann fold (alpha/beta folding pattern with a central beta-sheet), an NAD(P)(H)-binding region, and a structurally diverse C-terminal region. Classical SDRs are typically about 250 residues long, while extended SDRs are approximately 350 residues. Sequence identity between different SDR enzymes are typically in the 15-30% range, but the enzymes share the Rossmann fold NAD-binding motif and characteristic NAD-binding and catalytic sequence patterns. These enzymes catalyze a wide range of activities including the metabolism of steroids, cofactors, carbohydrates, lipids, aromatic compounds, and amino acids, and act in redox sensing. Classical SDRs have an TGXXX[AG]XG cofactor binding motif and a YXXXK active site motif, with the Tyr residue of the active site motif serving as a critical catalytic residue (Tyr-151, human 15-hydroxyprostaglandin dehydrogenase (15-PGDH) numbering). In addition to the Tyr and Lys, there is often an upstream Ser (Ser-138, 15-PGDH numbering) and/or an Asn (Asn-107, 15-PGDH numbering) contributing to the active site; while substrate binding is in the C-terminal region, which determines specificity. The standard reaction mechanism is a 4-pro-S hydride transfer and proton relay involving the conserved Tyr and Lys, a water molecule stabilized by Asn, and nicotinamide. Extended SDRs have additional elements in the C-terminal region, and typically have a TGXXGXXG cofactor binding motif. Complex (multidomain) SDRs such as ketoreductase domains of fatty acid synthase have a GGXGXXG NAD(P)-binding motif and an altered active site motif (YXXXN). Fungal type ketoacyl reductases have a TGXXXGX(1-2)G NAD(P)-binding motif. Some atypical SDRs have lost catalytic activity and/or have an unusual NAD(P)-binding motif and missing or unusual active site residues. Reactions catalyzed within the SDR family include isomerization, decarboxylation, epimerization, C=N bond reduction, dehydratase activity, dehalogenation, Enoyl-CoA reduction, and carbonyl-alcohol oxidoreduction.	2.32965e-90
NZ_CP032479.1|WP_139542902.1|556638_558036_-|cysteine--tRNA-ligase	gnl|CDD|234705	PRK00260, cysS, cysteinyl-tRNA synthetase; Validated.	0
NZ_CP032479.1|WP_139542892.1|537200_538766_-|flagellar-hook-length-control-protein-FliK	gnl|CDD|376734	pfam02120, Flg_hook, Flagellar hook-length control protein FliK. This is the C terminal domain of FliK. FliK controls the length of the flagellar hook by directly measuring the hook length as a molecular ruler. This family also includes YscP of the Yersinia type III secretion system, and equivalent proteins in other pathogenic bacterial type III secretion systems.	4.01481e-12
NZ_CP032479.1|WP_139542898.1|548770_549043_+|type-II-toxin-antitoxin-system-mRNA-interferase-toxin,-HP0892-family	gnl|CDD|225583	COG3041, COG3041, Uncharacterized protein conserved in bacteria [Function unknown].	5.30393e-37
NZ_CP032479.1|WP_139542903.1|558036_559497_-|murein-biosynthesis-integral-membrane-protein-MurJ	gnl|CDD|367301	pfam03023, MVIN, MviN-like protein. Deletion of the mviN virulence gene in Salmonella enterica serovar. Typhimurium greatly reduces virulence in a mouse model of typhoid-like disease. Open reading frames encoding homologs of MviN have since been identified in a variety of bacteria, including pathogens and non-pathogens and plant-symbionts. In the nitrogen-fixing symbiont Rhizobium tropici, mviN is required for motility. The MviM protein is predicted to be membrane-associated.	5.4951e-115
NZ_CP032479.1|WP_139542896.1|545494_547699_+|outer-membrane-beta-barrel-protein	gnl|CDD|375731	pfam18304, SabA_adhesion, SabA N-terminal extracellular adhesion domain. This is the N-terminal extracellular adhesion domain of Sialic acid binding adhesin (SabA) present in Helicobacter pylori. The N-terminal domain of SabA functions as a sugar-binding adhesion domain with conserved disulfide bonds. Notably, these amino acid residues are not only conserved among SabA orthologs but also between SabA and BabA.	1.18205e-111
NZ_CP032479.1|WP_139542891.1|536259_537150_-|flagellar-hook-assembly-protein-FlgD	gnl|CDD|235621	PRK05842, flgD, flagellar hook assembly protein FlgD.	3.33906e-175
NZ_CP032479.1|WP_033592703.1|549151_549676_-|acyl-CoA-thioesterase	gnl|CDD|224523	COG1607, COG1607, Acyl-CoA hydrolase [Lipid metabolism].	9.24635e-64
NZ_CP032479.1|WP_139542893.1|539956_540565_+|phosphate-acetyltransferase	gnl|CDD|235537	PRK05632, PRK05632, phosphate acetyltransferase; Reviewed.	1.65515e-122

Protein sequences

>NZ_CP032479.1|WP_139542896.1|545494_547699_+|outer-membrane-beta-barrel-protein
MKRHILSLTLGSLLVSTLSAEDDGFYMSAGYQIGEAAQMVANTKGIQQLSDNYEKLNNLLNNYSTLNTLIKLSADPSAVSGAINNLNAGATGLLKEKTNSPAYQAVSLALNAAVGLWNTIGYAVMCGNGNDTGSGPGSVVFNGEPGQHSTRITCNRYESTGPGKSMSIDEFKKLNEAYQIIQQALKNQSGFPELGGQGTSVNVNYEYECRQTDNNINGGVDQFCKAKNGSSGSNGSGGTSIQTTTQDGVKITTKYEDNKATVEFSITNNAQELLAQAANIMQVLNTQCPLVRSKYHEDVPGDGEPWGLSTSGNACQIFQQEFSQVTSMIKNAQEIVAQSKIANANQKAEIANPSNFNPFTDAGFAQGMLKNARAQASMFNLAEQVRKNLEVMENNNNVTGFGKGMTNFVSAFLASCKADGTLPNAGVTSDTWGAGCAYVEETITALTNSIAHFGTQAQQIQQAENIADTLVNFKSRYSELGNTYNSIATALSSIPNAQSLQNAVGKKNNPYSPQGIETNYYLNQNSYNQIQTINQELGRNPFRKVGIVSSQTNNGAMNGIGIQVGYKQFFGQKRKWGARYYGFFDYNHAFIKSSFFNSASDVWTYGFGADALYNFINDKATNFLGKNNKLSVGLFGGIALAGTSWLNSEYVNLATMNNVYNAKMNVANFQFLFNMGVRMNLARPKKKDSDHAAQHGIELGLKIPTINTNYYSFMGAELKYRRLYSVYLNYVFAY
>NZ_CP032479.1|WP_139542895.1|543394_544507_+|hydrogenase-formation-protein-HypD
MSVDHLISPFRDKQTLLALSNEIKKLASKLEKKLVIMEVCGGHTHSLMKYGLLDLMPKNLEFVHGPGCPVCVMPRARLDEAYELAMIKDSIILSLGDMMRVPGSYGSLIQAREKGLDARFLYSPMQALEIAKENPHKKVIYIAIGFETTTPMSASVLKNAQKEKINNLFFHINHILVPPSVSAILKDPACQINALLAPSHVSVISGAQIYAPLVDRFKIPIIVSGFEPVDILESVLMLIKQALNKEAKLEIQYKRAVSYEGNVKAQELVNACMEVRENFEWRGLGNIKHSALKLKEIFASYDAEKVFKEHLSHKTSKENKACKCGEILKGIAKPLDCSLFATTCTPQNPIGSCMVSSEGACAAYYRYKRV
>NZ_CP032479.1|WP_100969100.1|543155_543395_+|HypC/HybG/HupF-family-hydrogenase-formation-chaperone
MCLAIPSKVIAINDNVALLETLGVQREASLDLMGESVKVGDYVLLHIGYVMSKIDEKEALESIELYQEMIAKMNETHEQ
>NZ_CP032479.1|WP_000003606.1|542427_543156_+|hydrogenase-nickel-incorporation-protein-HypB
MSEQRKESLQNNPNLSKKDVKIVEKILSKNDIKAAEMKERYLKEGLYVLNFMSSPGSGKTTMLENLADFKDFKFCVVEGDLQTNRDADRLRSKGVSAHQITTGEACHLEASMIEGAFDLLKDEGALEKSDFLIIENVGNLVCPSSYNLGAAMNIVLLSVPEGDDKVLKYPTMFMCADAVIISKADMIEVFNFRVSQVKEDMQKLKPEAPIFLMSSKDPKSLEDFKNFLLEKKRENYQSTHSF
>NZ_CP032479.1|WP_000973846.1|542190_542319_+|hypothetical-protein
MMKAVFINVWYLLLTIILLIIIWRIIKLVLKDKNDSDDKGLG
>NZ_CP032479.1|WP_139542894.1|541880_542180_+|cupin-domain-containing-protein
MKMVHFLEGVHFEKLHIEALNENSSNKEIRICMPKGAVMDKHKAPGAISVQVLEGKIIFEVENEKIEMPKGALISLEAQVLHRLDALENSVIRLSLSKK
>NZ_CP032479.1|WP_139542893.1|539956_540565_+|phosphate-acetyltransferase
MPWFLGSITADTIRPALQIIKTKPGVSLVSSVFLMCLDTQVLVFGDCTIIPNPKELAEIATASAQTAKQFNIVPKVALLSYATGNSAQGEMIDKINEALTIAQKLDPQLEIDGPLQFDASIDKGVAKKKMPNSQVAGQASVFIFPDLNAGNIAYKAVQRSDKAVAIGPILQGLNKPINDLSRGALVEDIVNTVLISALQAQD
>NZ_CP032479.1|WP_139542892.1|537200_538766_-|flagellar-hook-length-control-protein-FliK
MPSPVNPIHTNASTNASALNSGAKNEDTKNAPKSASKDFSKILNQKISKDKTAPKENPNASKVTPKDAKEDAKTLKTPTLPHQHAKDLVKDQQAPTLKDWLNHKKTTASHETQHETHEANETNPKTPNETLNKNEKKPNGVTSNAHQTNLTHKNPLSPTNRANNAIKNPTTPTHNAKESKTLKDIQTLSQKHDLNASNIQVVASLEKKETPLNASDHLALKTTQTPTNHTLAKNDAKNTANLSSVLQSLEKKEPHNKEHANPQNNKKKTPPLKEALQMNAIKRDKTLSKKKPEKTPIHAKTQTTTPNATPENAPKIPLKTPPLMPLIGANPPPNDNPPTLLEKEETTKEVSDNKEKAKETSSSAQSAQNAQASDKTSENKSIAPKETIKHFTQQLKQEIQEYKPPMSRISMDLFPKELGKVEVIIQKVGKNLKVSVISHNNSLQTFLDNQQDLKNSLNALGFEGVDLSFSQDSSKEQPKEQLRELFKEQESSPLKENALKSYQENTDHENQETSMQITLYA
>NZ_CP032479.1|WP_139542891.1|536259_537150_-|flagellar-hook-assembly-protein-FlgD
MAIDLAEVTGAKAAQERKKEQPTIANGLDKNAFMKLFLEQLKNQDPTAPMETDKIITQTAQLTQVEMQEENKKTMQEVASAMKSNKETNESLKDFQGTLKDTMENLNKGMDDSLKANNALREVTALNSVSMIGKIAETDVSGANFDGNNKLSFSLFFDEKIDASKGVPAIQILNENNELVKTIPLKDYNGQKGYINFEWDGTNEKGEKVPKGNYKIKAEYNLDSHSKQYLQTRIGRGEVESVIFDKGKPMLRMGEMILPIDSAIEFYKPDQKPLEQKLSDQRPLEQKPQTPPKETA
>NZ_CP032479.1|WP_139542890.1|534445_536263_-|flagellar-hook-basal-body-complex-protein
MNDTLLNAYSGIKTHQFGIDSLSNNIANVNTLGYRSNDPEFKTLFSSHLDALNAKSVVANDRNYGVTGSGNTLSNKDGEYMPSEGEFHMAYQGKGWFVIGPNKNGEMTINKDGFSKKQDNFLTRAGNFARDADGYLVTPEGYYVYGIDLKKIKDGTLNSTARDEDIEKLHGNTLSPLQIPQDLTYQPVLSTKVGISVNLNPKDHLKGVQDFFLNDKGEIIKERFLNQDINALANDDNEPIDAITNRKLNISIQKEDGKKEDFVFTYGDAEKGENQFKTLGDLQKLIKEKTGLDLNLIKSEKDAKSPPLLLEIANPSETPITFSLSGGIADKLGLKADGMELKKGISRDSVAIKIPYYSTEVDIYDKAGDKYLLQSEYYMTNSNDPTSSPTSKRKNQTWEVKSYIVDPKNKTPINDPTWEIVGFDSATHKMKSAPMTLDFKGNKLTYSLDKSENHDSSDLSYQDSKLLEASQDGKPRGIFRDMRIEENGVISLAFSNGVVEPVARIGILAFTNDQGLRKIGGNLYEMQEGTINGENRPLSGNPILGWDEEGKLKFGKIRHKYLETSNVNAGNALTNLILMQRGYSMNARAFGAGDDMIKEAISLKK
>NZ_CP032479.1|WP_139542897.1|548472_548784_+|hypothetical-protein
MPNTTAKKDYTKYSQRQLFNFLNSIKTKQKRALEKLKEIQAQKQRIKKALQFKALHLTENGYTIEEEREILARAKDTKNHLCFKSVEDFKKHCENLRFKNADD
>NZ_CP032479.1|WP_139542898.1|548770_549043_+|type-II-toxin-antitoxin-system-mRNA-interferase-toxin,-HP0892-family
MLTIETSKKFDKDLKILVKNGFDLKLLYKVVENLAKEQPLAPKYKDHPLKGGLKDFRECHLKPDLLLVYQIKKQENTLFLVRLGSHSELF
>NZ_CP032479.1|WP_033592703.1|549151_549676_-|acyl-CoA-thioesterase
MPQIQSSHSSHFDFTIDTADRTKLLMSYLVVPTTANFNNVMHGGELLNLLDKVAYVCSTRYCAKGTVTLSVDGVTFKYPIPVGNLLTFLASINYVGNTSCEVGIKVLSEDIKTREITHTNSCYFTMVAVENGKPTPMPKYEPKTEVEIRRYEGALKRKEMRMQGYLKSGKHEGV
>NZ_CP032479.1|WP_139542899.1|549815_550664_+|SDR-family-NAD(P)-dependent-oxidoreductase
MGEKKESQKVAVITGASSGIGLECALMLLDQGYKVYALSRHATLCVALNHALCESVDIDVSDSNALKEVFLNISAKEDHCDVLINSAGYGVFGSVEDTPIEEVKKQFGVNFFALCEVVQFCLPLLKNKPYSKIFNLSSIAGRVSMLFLGHYSASKHALEAYSDALRLELKPFNVQVCLIEPGPVKSNWEKTAFSVENFEGEDSLYALEVNAAKTFYASVYENALSAKVVAQKIVFLSMSQKIKARYLIGLKTQLLLALYRILPSSWYDSLFRLIVLKRKRDA
>NZ_CP032479.1|WP_000921449.1|550656_551637_+|iron-ABC-transporter-permease
MLKTYHIALACVILAVVVLLFGGESLSFEEWQEVCLNVKNHFLHNEELSSLSIIILEIRLPRVILALLVGASLSGSGVVMQTIFRNPLVDPFLLGISSGAMLGVAMAIAVVESNIAILAFFGAILASLAVLAMNRVLGNSVLSLVLSGVVLSAFLSALAGAIKFFVIPQKAQAIVVWLLGSLSLSSYKDCLIAFIGLSLGFIPLFLLRWRINLLSLSDAQSLSLGINPVLLRSLCLVCVSVASALAVSVSGTIGWIGLVIPHVARLFFGANLQKLLLSSLLMGAFFLLLADVVAKTITPYDLPVGIATSVLGAPFFLWLLFRTRGV
>NZ_CP032479.1|WP_139542900.1|551636_552404_+|ATP-binding-cassette-domain-containing-protein
MVLEVKNLSFKYSQKLILDKLSFSVPKNSITSILAPNGSGKTTLLKCLLGLLRPLEETEIKACNKDILPLKPYEKAKLIAYIPQVEYYAFNFSVLDFVLMGKATHLNLFAMPKAKHIKEATSVLERLDLESLKDQGINDLSGGQRQMVLLARSLLQRTPLLLLDEPTSALDLKNQALFFDAIKDEMKKRELSVLVNIHDPNLVARHSTHVVMLKDKKLFLQASTPIAMTSHNLSALYDTPLEAIWHDNKLVVYAL
>NZ_CP032479.1|WP_139542901.1|552474_556416_-|autotransporter-outer-membrane-beta-barrel-domain-containing-protein
MEIQQTHRKINRPIISLALVGVLIGTELGANTPNDPIHSESRAFFTTVIIPAIVGGIATGAAVGTVSGLLSWGLKQAEQANKAPDKPDKVWRIQAGRGFDNFPHKQYDLYKSLLSSKIDGGWDWGNAARHYWVKDGQWNKLEVDMQNAVGTYNLSGLINFTGGDLDVNMQKATLRLGQFNGNSFTSFKDSADRTTRVNFDAKNILIDNFVEINNRVGSGAGRKASSTVLTLKSSEKITSRENAEISLYDGATLNLVSSSNQSVDLYGKVWMGRLQYVGAYLAPSYSTINTSKVTGEMNFRHLAVGDQNAAQAGIIANKKTNIGTLDLWQSAGLSIITPPEGGYESKTKDTPSQNNPKNDTQKTEIQPTQVIDGPFAGGKDTVVNIFHLNTKADGTIRAGGFKASLSTNAAHLHIGEGGINLSNQASGRTLLVENLTGNITVEGTLRVNNQVGGSAVAGSSANFEFKAGEDTNNATATFNNDIHLGKAVNLRVDAHTAYFNGNIYLGKSTNLRVNGHSAHFKNIDATKSDNGLNTSALDFSGVTDKVNINKLTTSATNVNVKNFDIKELVVTTRVQSFGQYTIFGENIGDKSRIGVVSLQTGYSPAYSGGVTFKSGKKLVIDEIYHAPWNYFDARNVTDVEINKRILFGAPGNIAGKTGLMFNNLTLNSNASMDYGKDLDLTIQGHFTNNQGTMNLFVQDGRVATLNAGHQASMIFNNLVDSATGFYKPLIKVNNAQNLTKNKEHVLVKARNIDYNLVGVQGASYDNISASNTNLQEQFKEHLALYNNNNRMDICVVRKDNLNDIKACGMAIGNQSMVNNPENYKYLEGKAWKNTGINKTANNTTIAVNLGNNSTPTSSESNTTNLPTNTTNNARFASYALVKNAPFARYSATPNLVAINQHDFGTIESVFELANRSSDIDTLYTHSGAQGRDLLQTLLIDSHDAGYARTMIDATSANEITKQLNAATTTLNNIASLEHKTSGLQTLSLSNAMILNSRLVNLSRRHTNNIDSFAKRLQALKDQRFASLESAAEVLYQFAPKYEKPTNVWANAIGGTSLNSGGNASLYGTSAGVDAYLNGEVEAIVGGFGSYGYSSFSNQANSLNSGANNTNFGVYSRIFANQHEFDFEAQGVLGSDQSSLNFKSALLQDLNQSYHYLAYSAATRASYGYDFAFFRNALVLKPSVGVSYNHLGSTNFKSNSTNQVALKNGSSSQHLFNASANVEARYYYGDTSYFYMNAGVLQEFAHVGSNNAASLNTFKVNAARNPLNTHARVMMGGELQLAKEVFLNLGVVYLHNLISNASHFASNLGMRYSF
>NZ_CP032479.1|WP_139542902.1|556638_558036_-|cysteine--tRNA-ligase
MFIYDTKSKQKVPFEPLVKNKANIYVCGPTVYDDAHLGHARSAIAFDLLRRTLELSGYEVMLVRNFTDIDDKIINKALKENKSIQELSSIYIESYTRDLNALNVKKPSLEPKASEYLDAMVRMIETLLEKNFAYRVSNGDIYLDTSKDKDYGSLSVHNSSMEFGRIGLVQEKRLEQDFVLWKSYKGDNDVGFDSPLGKGRPGWHIECSSMVFETLALTNTPYQIDIHAGGADLLFPHHENEACQTRCAFGVEIAKYWMHNGFVNINNEKMSKSLGNSFFIKDALKNYDGEILRNYLLGVHYRSVLNFNEEDLLVSKKRLDKIYRLKQRVLGTLGGINPNFKKEILECMQDDLNVSKALSVLESMLSSTNEKLDQNPKNKALKGEILANLKFVEELLGIGFKDPSAYFQLGVSESEKQEIEKKIEERKRAKEQKDFLKADSIREELLNHKIALMDTPQGTIWEKLF
>NZ_CP032479.1|WP_139542903.1|558036_559497_-|murein-biosynthesis-integral-membrane-protein-MurJ
MLKKIFLTNSLGILCSRIFGFLRDLMMANILGAGVYSDIFFVAFKLPNLFRRIFAEGSFSQSFLPSFIRSSIKGSFAGLVGLIFCGVLFIWCLLVALNPLWLTKLLAYGFDEETLKLCAPIVAINFWYLLLVFITTFLGTLLQYKHSFFASAYSASLLNLCMILALFISKEKTHLEALYYLSYGVLLGGVAQILLHFYPLVKLGLLNLLFKGFLSFKTKNANKKEYRLNRVKKDLKGFFKQFLPSVLGNSSAQIASFLDTTIASFLASGSVSYLYYANRVFQLPLALFAIAISTALFPSIAIAIKNHQQDLILKRLQKAWFFLVGVLLLCSIGGIMLSKEITELLFERGQFSPKDTLITSQVFSLYLLGLLPFGLTKLFSLWLYAKLEQKKAAKISLISLFLGLAASLSLMPLLGVLGLALANSLSGLFLFVLTIKAFGIQAFLDIIKNLKLWLVILFLACVEILLLLAFKSWVTHLYLFYYFQGF
>NZ_CP032479.1|WP_139542904.1|559588_561433_+|DUF342-domain-containing-protein
MSLEHFAPIKVERCKDIQKELKKAAAENKLQTEDLWFEILKTSIFIKNSAKDDFSEAFGGELQQLEEDEYYEKKELTLYQTHDIKIKSNAYKRFFEVEVDEDLSKIEIILDECFIVLDTEEHYQEMFAYIKECLAFQGVVFRHLSQMYENLKTELRKYKKEAQNKHFILYASLTFIPNIEEQSHFLLEEEYLPTHTIFLSDQEESFVKENYYIAKENQKVACVNYPKQGRDGRNLKGLYIELPKVAHSPTPIGHDKNAFEEREENNALVYYSKALQGVKMEKGRLVSKQNFIFKNGIKSIETPNLLGGVESGLVLEIQAKDELSDAIDSNLILEASVINIKGNVGKNVILVAKEITIEGQIHPESYVYANKVRITNHKGVCYAKEFECKYLERAKVYANSVKVEASAGSVVYAKEIALEKLKSDNKLYFSKQCWIDEVDGNGNRFIFYAFGGRENQEELKIAKQKLNALGLKSKKIIAQHQSLNHLVKNHQAIMEKLKNATEEIKRSLMQQESVKDAYSEFMFALKRLKILKAQMLELQKINNECYAKLISIENSFQHASITTKNPFKQENIVIYHRNYPKVSNLSAMLSHNESVNVIYEDHKIKKVPKSTIKG

Crispr_ID: NZ_CP032479_3

• CRISPR_ID: NZ_CP032479_3
• CRISPR_location: 1516779-1517040
• CRISPR_type: Orphan
• Repeat_type: NA
• Spacer_info: 2 spacers
• Cas_protein_info: DEDDh

Consensus repeat of NZ_CP032479_3

Consensus_repeat	Method
TCACCATAGTTTAACTCGCAAGCTTTAGAGTAATATTCAAAAGCTTTTTTAA	PILER-CR

spacers of NZ_CP032479_3

>3.1|1516831|56|NZ_CP032479|PILER-CR
GATCTTGCGTTACGCTTTCACCAAGAAAATAATCCCCTCCTAATTTAGAACACCCT
>3.2|1516939|56|NZ_CP032479|PILER-CR
AATTTTGTGTTACGCCTTCACCATTATGATAAATATCCCCTAAAATCGCACAGCCA

CRISPR arrays and Neighbor proteins around NZ_CP032479_3

CRISPR arrays

The CRISPR arrays of NZ_CP032479_3

>merge|NZ_CP032479|3|1516779-1517040|PILER-CR
TCGTTTAATTCGCAAGCTTTAGAGTAATATCCAAAAGCTTTTTTAAGATCTTGCGTTACGCTTTCACCAAGAAAATAATCCCCTCCTAATTTAGAACACCCTTCACCATTATTCAACTTACAAGCTTTAGAGTAATATTTGAAAGCTTTTTTAAAATTTTGTGTTACGCCTTCACCATTATGATAAATATCCCCTAAAATCGCACAGCCATCGCCATAGTTTAACTCGCAAGCTTTAGAGTAATATTCTAGATCTTTTTTAT

>NZ_CP032479|3|1|1516779-1517040|PILER-CR
TCGTTTAATTCGCAAGCTTTAGAGTAATATCCAAAAGCTTTTTTAAGATCTT	GCGTTACGCTTTCACCAAGAAAATAATCCCCTCCTAATTTAGAACACCCTTCACCA
TTATTCAACTTACAAGCTTTAGAGTAATATTTGAAAGCTTTTTTAAAATTTT	GTGTTACGCCTTCACCATTATGATAAATATCCCCTAAAATCGCACAGCCATCGCCA
TAGTTTAACTCGCAAGCTTTAGAGTAATATTCTAGATCTTTTTTAT

Neighbor Proteins Overview

Protein	Signature genes	Signature genes Name	Protein_function
NZ_CP032479.1|WP_139543506.1|1512209_1512941_-|type-II-restriction-endonuclease	unknown	unknown	unknown
NZ_CP032479.1|WP_139543511.1|1517953_1519261_-|DUF814-domain-containing-protein	unknown	unknown	gnl|CDD|224212
NZ_CP032479.1|WP_139543518.1|1528552_1531078_+|TonB-dependent-receptor-plug-domain-containing-protein	unknown	unknown	gnl|CDD|227113
NZ_CP032479.1|WP_139543507.1|1512933_1513830_-|SAM-dependent-methyltransferase	unknown	unknown	unknown
NZ_CP032479.1|WP_139543513.1|1520844_1521699_-|NAD(+)/NADH-kinase	unknown	unknown	gnl|CDD|223139
NZ_CP032479.1|WP_139543515.1|1524515_1525613_-|alcohol-dehydrogenase-catalytic-domain-containing-protein	unknown	unknown	gnl|CDD|176186
NZ_CP032479.1|WP_139543505.1|1510996_1512064_+|elongation-factor-Ts	unknown	unknown	gnl|CDD|236491
NZ_CP032479.1|WP_000258276.1|1510202_1510997_+|30S-ribosomal-protein-S2	unknown	unknown	gnl|CDD|235396
NZ_CP032479.1|WP_078215307.1|1521884_1522613_+|outer-membrane-protein	unknown	unknown	gnl|CDD|280099
NZ_CP032479.1|WP_139543508.1|1514045_1514918_-|class-1-fructose-bisphosphatase	unknown	unknown	gnl|CDD|223236
NZ_CP032479.1|WP_139543512.1|1519257_1520832_-|DNA-repair-protein-RecN	unknown	unknown	gnl|CDD|223571
NZ_CP032479.1|WP_139543503.1|1505762_1507079_-|sodium/proton-antiporter-NhaA	unknown	unknown	gnl|CDD|184858
NZ_CP032479.1|WP_139543509.1|1514988_1515642_+|ribulose-phosphate-3-epimerase	unknown	unknown	gnl|CDD|273475
NZ_CP032479.1|WP_139543514.1|1522623_1524384_-|DUF262-domain-containing-protein	unknown	unknown	gnl|CDD|224396
NZ_CP032479.1|WP_000539101.1|1517637_1517934_-|hypothetical-protein	unknown	unknown	unknown
NZ_CP032479.1|WP_139543504.1|1507102_1509952_-|RecB-like-helicase	unknown	unknown	gnl|CDD|237554
NZ_CP032479.1|WP_128062418.1|1513842_1514049_-|hypothetical-protein	unknown	unknown	unknown
NZ_CP032479.1|WP_139543517.1|1527228_1528197_+|arginase	unknown	unknown	gnl|CDD|162262
NZ_CP032479.1|WP_120898811.1|1515619_1516483_+|3'-5'-exonuclease	DEDDh	cd06127_DEDDh_CAS-I	gnl|CDD|236281
NZ_CP032479.1|WP_139543516.1|1525882_1527025_-|NAD-binding-protein	unknown	unknown	gnl|CDD|223758

HMMScan for Signature genes

Protein	Cas_name	Cas_description	E-value	Identity	Coverage
NZ_CP032479.1|WP_120898811.1|1515619_1516483_+|3'-5'-exonuclease	DEDDh	cd06127_DEDDh_CAS-I	4e-37	122.5	0.0

rpsblast for functional proteins

Protein	Function_ID	Function_description	E-value
NZ_CP032479.1|WP_139543511.1|1517953_1519261_-|DUF814-domain-containing-protein	gnl|CDD|224212	COG1293, COG1293, Predicted RNA-binding protein homologous to eukaryotic snRNP [Transcription].	1.99758e-43
NZ_CP032479.1|WP_139543518.1|1528552_1531078_+|TonB-dependent-receptor-plug-domain-containing-protein	gnl|CDD|227113	COG4772, FecA, Outer membrane receptor for Fe3+-dicitrate [Inorganic ion transport and metabolism].	0
NZ_CP032479.1|WP_139543513.1|1520844_1521699_-|NAD(+)/NADH-kinase	gnl|CDD|223139	COG0061, nadF, NAD kinase [Coenzyme metabolism].	9.04355e-79
NZ_CP032479.1|WP_139543515.1|1524515_1525613_-|alcohol-dehydrogenase-catalytic-domain-containing-protein	gnl|CDD|176186	cd05283, CAD1, Cinnamyl alcohol dehydrogenases (CAD). Cinnamyl alcohol dehydrogenases (CAD), members of the medium chain dehydrogenase/reductase family, reduce cinnamaldehydes to cinnamyl alcohols in the last step of monolignal metabolism in plant cells walls. CAD binds 2 zinc ions and is NADPH- dependent. CAD family members are also found in non-plant species, e.g. in yeast where they have an aldehyde reductase activity. The medium chain dehydrogenases/reductase (MDR)/zinc-dependent alcohol dehydrogenase-like family, which contains the zinc-dependent alcohol dehydrogenase (ADH-Zn) and related proteins, is a diverse group of proteins related to the first identified member, class I mammalian ADH. MDRs display a broad range of activities and are distinguished from the smaller short chain dehydrogenases (~ 250 amino acids vs. the ~ 350 amino acids of the MDR). The MDR proteins have 2 domains: a C-terminal NAD(P) binding-Rossmann fold domain of a beta-alpha form and an N-terminal catalytic domain with distant homology to GroES. The MDR group contains a host of activities, including the founding alcohol dehydrogenase (ADH), quinone reductase, sorbitol dehydrogenase, formaldehyde dehydrogenase, butanediol DH, ketose reductase, cinnamyl reductase, and numerous others. The zinc-dependent alcohol dehydrogenases (ADHs) catalyze the NAD(P)(H)-dependent interconversion of alcohols to aldehydes or ketones. Active site zinc has a catalytic role, while structural zinc aids in stability. ADH-like proteins typically form dimers (typically higher plants, mammals) or tetramers (yeast, bacteria), and generally have 2 tightly bound zinc atoms per subunit. The active site zinc is coordinated by a histidine, two cysteines, and a water molecule. The second zinc seems to play a structural role, affects subunit interactions, and is typically coordinated by 4 cysteines.	3.01719e-155
NZ_CP032479.1|WP_139543504.1|1507102_1509952_-|RecB-like-helicase	gnl|CDD|237554	PRK13909, PRK13909, RecB-like helicase.	0
NZ_CP032479.1|WP_000258276.1|1510202_1510997_+|30S-ribosomal-protein-S2	gnl|CDD|235396	PRK05299, rpsB, 30S ribosomal protein S2; Provisional.	6.12541e-155
NZ_CP032479.1|WP_139543508.1|1514045_1514918_-|class-1-fructose-bisphosphatase	gnl|CDD|223236	COG0158, Fbp, Fructose-1,6-bisphosphatase [Carbohydrate transport and metabolism].	4.58812e-113
NZ_CP032479.1|WP_139543512.1|1519257_1520832_-|DNA-repair-protein-RecN	gnl|CDD|223571	COG0497, RecN, ATPase involved in DNA repair [DNA replication, recombination, and repair].	2.5816e-129
NZ_CP032479.1|WP_139543503.1|1505762_1507079_-|sodium/proton-antiporter-NhaA	gnl|CDD|184858	PRK14856, nhaA, sodium/proton antiporter NhaA.	0
NZ_CP032479.1|WP_139543509.1|1514988_1515642_+|ribulose-phosphate-3-epimerase	gnl|CDD|273475	TIGR01163, Ribulose-phosphate_3-epimerase, ribulose-phosphate 3-epimerase. This family consists of Ribulose-phosphate 3-epimerase, also known as pentose-5-phosphate 3-epimerase (PPE). PPE converts D-ribulose 5-phosphate into D-xylulose 5-phosphate in Calvin's reductive pentose phosphate cycle. It has been found in a wide range of bacteria, archebacteria, fungi and plants. [Energy metabolism, Pentose phosphate pathway].	2.59689e-117
NZ_CP032479.1|WP_139543514.1|1522623_1524384_-|DUF262-domain-containing-protein	gnl|CDD|224396	COG1479, COG1479, Uncharacterized conserved protein [Function unknown].	5.32963e-27
NZ_CP032479.1|WP_078215307.1|1521884_1522613_+|outer-membrane-protein	gnl|CDD|280099	pfam01856, HP_OMP, Helicobacter outer membrane protein. This family seems confined to Helicobacter. It is predicted to be an outer membrane protein based on its pattern of alternating hydrophobic amino acids similar to porins.	6.66579e-47
NZ_CP032479.1|WP_139543505.1|1510996_1512064_+|elongation-factor-Ts	gnl|CDD|236491	PRK09377, tsf, elongation factor Ts; Provisional.	1.84138e-125
NZ_CP032479.1|WP_139543517.1|1527228_1528197_+|arginase	gnl|CDD|162262	TIGR01229, rocF_arginase, arginase. This model helps resolve arginases from known and putative agmatinases, formiminoglutamases, and other related proteins of unknown specifity. The pathway from arginine to the polyamine putrescine may procede by hydrolysis to remove urea (arginase) followed by decarboxylation (ornithine decarboxylase), or by decarboxylation first (arginine decarboxylase) followed by removal of urea (agmatinase).	1.83977e-138
NZ_CP032479.1|WP_120898811.1|1515619_1516483_+|3'-5'-exonuclease	gnl|CDD|236281	PRK08517, PRK08517, 3'-5' exonuclease.	5.41595e-137
NZ_CP032479.1|WP_139543516.1|1525882_1527025_-|NAD-binding-protein	gnl|CDD|223758	COG0686, Ald, Alanine dehydrogenase [Amino acid transport and metabolism].	2.07787e-173

Protein sequences

>NZ_CP032479.1|WP_120898811.1|1515619_1516483_+|3'-5'-exonuclease
MPINLLHKDIQALIARLKHQDLSLGMLEKSLSRLIYDEINLEYLKACGLNFIETSENLITLKNLKTPLKDEVFSFIDLETTGSCPLKHEILEIGAVQVKGGEIINRFETLVKVKSVPDYIAELTGIAYEDTLNAPSVHEALQELRLFLGNSVFVAHNANFDYNFLGRYFVEKLHCPLLNLKLCTLDLSKRAILSMRYSLSFLKELLGFGIEVSHRAYADALASYKLFEICLLNLPSYIKTTMDLIDFSKCANTLIKRPPRSKYQETPPPFSLFERTKGLFNIIKATS
>NZ_CP032479.1|WP_139543509.1|1514988_1515642_+|ribulose-phosphate-3-epimerase
MKVAPSLLSADFMHLAKEIESVSNADFLHVDVMDGHYVPNLTMGPVVLENVTQMSQLPLDVHLMVENASFFVELFAPLKPQIISIHAENEKHPHRVLQLIKNSGITPGIVLNPHTHEESVKYLLESVGLVLLMSVNPGFGGQKFLDLVLEKCLKVKELIKRYNPSCLLEVDGGVNDKNIFELQQAGVDVVVSGSYIFKSKDRKLAIEGLQNANKSLA
>NZ_CP032479.1|WP_139543508.1|1514045_1514918_-|class-1-fructose-bisphosphatase
MDYKHFKCKHANIVIEIISLLEKGVKKAQEILEKPDAGSYTKLENSSGDTPIKADLALDKFLEENFLSLENIKSVFSEEKETPVTKENGSYLIAYDPLDGSSVMEANFLVGTIIGVYEKDYKAQNLVASLYVVFGHKIELVVALDKVYRYAFYQNKFHFIETIVLENKGKIVASGGNQKDFSLGLKKALEGFFAENYRLRYSGSMVADVHHVLIKKGGMFSYPQKKLRKLFEVFPLALMVEKAKGEAFYFDKGVKKRLLEQSVESYHEKSECYLASPHEAHILEKYLKGE
>NZ_CP032479.1|WP_128062418.1|1513842_1514049_-|hypothetical-protein
MMQNSTKKLEYEERFNDALLKLKACQEEKQVTSCLKCEKVLKCEIRNSYVDAAYESMSLGEAGGFDFN
>NZ_CP032479.1|WP_139543507.1|1512933_1513830_-|SAM-dependent-methyltransferase
MVSNTILQKNLDAFYTHPKIARFCLDLLRDLIVQNLGLDLNVFHFLEPSAGSGSFVGALKGLGIADWDALDIAPKAQGIQKKDYLLELIEFDKKRVIIGNPPFGHRGKLALDFLNKSLNEAPIVAFILPNLFKRYSIQKHIDKCAKLVLNVPLEKNAFIFNERPYDVKCVFQIYMHKNIALNLKDERIIAPPKIRHSDFITYIHNNTPHTLKYFNKEKYQWDFAVVRQGFYDYNEKITNANLLIKNRQYFFIKAHSKEALRIIHKIDFNKLAHKNTQVLGFSTYDFVEEYCKLKEMHA
>NZ_CP032479.1|WP_139543506.1|1512209_1512941_-|type-II-restriction-endonuclease
MLEKVFQEITNKRKFFASSSTGEQFENQFRNELKKLFSEINGNLTEELSHIEEKPNKEIKTTFNQLKKQVLEKNYPHTLKNPFSNLTSHFLYQPFGSQNYPDFLVFIFDHVVGIEIKFSKNDKGEKNLQTSRPMWNSNLPKPNAIYVYGVANADITFFKGSDILSYETREVLLKYFDTLDKDEGNLKNALKDLENPFGFAPYIRKAYEHKKEFSNHHQIESFFSPNHILREQNVLKFLKTLTH
>NZ_CP032479.1|WP_139543505.1|1510996_1512064_+|elongation-factor-Ts
MSGISAQLVKKLRDLTDAGMMDCKKALVEVAGDLQKAIDFLREKGLSKAAKKADRIAAEGVVALEVAPDFKSAMMVEINSETDFVAKNEGFKELVKKTLETIKAHNIHTTEELLKSPLDNKPFEEYLHSQIAVIGENILVRKIAHLKAPSSHIINGYAHSNARVGVLIGIKYNNEKNAPKVVELARNIAMHAAAMKPQVLDCKDFSLDFVKKETLALIAEIEKDNEEAKRLGKPLKNIPTFGSRIELSDEVLAHQKKAFENELKEQGKPEKIWDKIVPGKMERFIADNTLIDQRLTLLGQFYVMDDKKTIAQVIADCSKEWDDDLKITEYARFELGEGIEKKAENFAEEVALQMK
>NZ_CP032479.1|WP_000258276.1|1510202_1510997_+|30S-ribosomal-protein-S2
MVTMKDLLECGVHFGHQTRRWNPKTKKFIFGVRKNIHIIDLQKTLRYFRYTYNIVRDASAQGKSIMFVGTKKQANETLKEFAESIQVPYVNYRWLGGMLTNFSTIRKSVRKLEIIEEMENSGQIDLLTKKEKLMILRKKEKLDKYLGGVRHMKKIPDMIFVIDVAKEKIAVAEARKLHIPIVAPLDTNCDPDLVDYPIPGNDDAIRSIRLFCKEMSEAILEGRELMQEEIVHADENSEEIEFVSNEEKEEMLAEIQKEITQGAE
>NZ_CP032479.1|WP_139543504.1|1507102_1509952_-|RecB-like-helicase
MDTKRQCMALKASAGSGKTFALSVRFLALLFKGANPSEILTLTFTKKATAEMKERILDYLKILQQENLENEKEKAQNILKELEEKYHLDPSLVQNSTQKIYQRFLNAEIRISTIDAFFQSILRKFCWFVGLSANFEVNEDTKAHQQQLNEGFLSALNGEQLEELSVFIAQCLSYDSYTSDSILERLRFLKNKLYLFDPNKKEPAFDEKGFLEKLRSLNEQIQSMETASDRAKTAIKCDSFRGFLNSSLTWLEKKSEYQSFKKLKSEIPTLESECEEIENDLKRYYEAKETAIFKKFPKFIQLYNNATSKIQALDFDAIKDKVHVLLNGYEEMPAEFFYFRLDSKIAHILIDEFQDTSLNDYKILAPFIDEIKAGIGQAKWQRSVFFVGDVKQSIYAFRGSFSSLFESVAKDFYHDNLQFNHRSAPLIINYVNTIFKKAYQNSPTAYLEQKYPKTSQNKHATDGYVKVSLVADERELLLDQVLQEAQNLLEHRIEPKDITILCATNKDALEVKNYLQERLSAIRPSTESSARLSQFVESKIIKNALKYALAEEPYKPFYKHSVLKLAGYLHDDVIALPGFNPKKESVAGFVWKVMEQFELYGEPAQSCLELAVGCEDADGFLEKLEAKSIASSHSKGAQIMTIHKSKGMQFPYVIVCERLGKPNSSHSNQLLEEYNGTELLRLYYRMKNREVVDKDYARALDKEEAAKNHEETNVYYVAFTRAELGLIVVAKDKKESKKESKQESKNKTMREKLDLVPLEEGEIAPVISPQKEPLITSALIKPHAYGEQVQEIEEEPSDYEKNNDQEAINFGIALHKGLEYQYAYNIPKQSVLEYLNYHYGFYSLDYQALEESLELFENDAEIQALFKNYFLKGEAAFLLEGVVSRIDVLLWDRGQNLYVLDYKSSQNYQQSHKVQVSHYAEFLKTQAPHFKIQAGIIYAHKRLLEKLWV
>NZ_CP032479.1|WP_139543503.1|1505762_1507079_-|sodium/proton-antiporter-NhaA
MNLKKTENALSVTLKSFIKSESFGGIFLFLNAVLAMVVANSFLKESYFALWHTPFGFQIGDFFIGFSLHNWIDDVLMALFFLMIGLEIKRELLFGELSSFKKASFPVIAALGGMIAPGLIYFFLNANTPSQHGFGIPMATDIAFALGVIMLLGKRVPTALKVFLITLAVADDLGAIVVIALFYTTNLKFAWLLGALGVVLVLAVLNRLNMRSLIPYLLLGVLLWFCVHESGIHATIAAVILAFMIPVKIPKDSKNVELLELGKRYAETSSGALLSKEQQEILHSIEEKASALQSPLERLEHFLAPISGYFIMPLFAFANAGVSVDSSINLEVDKVLLGVILGLCLGKPLGIFLITFISEKLKITARPKGISWWHILGAGLLAGIGFTMSMFISNLAFTSEHKDAMEVAKIAILLGSLISGIIGALYLFALDKRAALKK
>NZ_CP032479.1|WP_000539101.1|1517637_1517934_-|hypothetical-protein
MGVSALGNVTYINQNAPLNSAIQQGARSDMLDLAQSFQSKLELAQETRALENMQAISDKDPKEGSKNRYPNSRRAKAFGLEEEEIELWHEEHLLDIVG
>NZ_CP032479.1|WP_139543511.1|1517953_1519261_-|DUF814-domain-containing-protein
MKFFLLKKFSEFLNAQTHFSLKRLSASSFLLETFSKEKHAFIVDLSAPYIGLSKKPPESVLKNTLALDFCLNKFTKNAKILQANIIDNDRILEITGAKDLAYKSENFILRLEMIPKKANLMILDKEKCVIEAFRFNDRVAKNDILGALPPNTYEHQEEDLDFKGLLEILEKDFLSYQHKELEHKKNQIIKRLNIQKERLKEKLEKLENPKNLQLEAKELQTQAQLLLTYQHLIHRHESRVVLKDFEDKECAIEIDKSMPLNAFINKKFTLSKKKKQKSQFLHLEEENLKEKIAFKENQINYVKGVQEESVLEMFMPFKNSKIKRPMNGYEVLYYKDFKIGLGKNQKENIKLLQDARANDLWMHVRDIPGSHLIVFCQKNTPKDEVIMELAKMLIKMQKDAFNSYEIDYTQRKFVKIIKGANVIYSKYRTISLKDT
>NZ_CP032479.1|WP_139543512.1|1519257_1520832_-|DNA-repair-protein-RecN
MRDFNNAQITRLKVRQNAVFEKLDLEFKDGLSAISGASGVGKSVLIASLLGAFGLKESNASNIEVELIAPFLDTEEYGIFREDNHEPLVISVIKKEKTRYFLNQTSLSKNTLKALLKGLIKRLSNDRFSQNELNDILMLSLLDGYIQNENEAFSPLLGALEEKFTRLEKLEKERRLLEDKKRFQKDLEERLNFEKMKLERLDLKEDEYERLLEQKKLLSSKEKLNDKIALALEVLENTHKITHALESVGHSAEFLKSALLEASALLEKEQAKLEECERLDIEKVLERLGMLSGIIKDYGSIMRAKERLGHVKNELHNLKEIDHHCETYHKEIEQLKTECLKLCEEISGFRKEYLAGFNALLSAKAKDLLLKSPSLVLEDAPMSEKGAQKLVLNLQNSQLETLSSGEYSRLRLAFMLLEMEFLKDFKGVLVLDEMDSNLSGEESLAVSKALETLSSHSQIFAISHQVHIPALAKNHILVFKENHKSLAKTLNNEERVLEIARMIGGSENIESAISFAKEKLKAQE
>NZ_CP032479.1|WP_139543513.1|1520844_1521699_-|NAD(+)/NADH-kinase
MKDSHQTIGVFVRPTHYQNPLFKELEQAKEWVLKLLEDEGFESFMIDSLDGAKDARLIEKAYAFLCLGGDGTILGALRMTHSYNKPCFGVRIGNLGFLSAVELSGLKDFLQDLKQNRIKLEEHLALEGRIGKISFYAINEIVIAKKKALGVLDIKAYAGHTPFNTYKGDGLIIATPLGSTAYNLSAHGPIVHALSQSYILTPLCDFSLTQRPLVLGAEFCLNFCAHEDALVVIDGQATYDLKANQPLYIQKSPTTTKLLQKNSRDYFKVLKEKLLWGESPSKKK
>NZ_CP032479.1|WP_078215307.1|1521884_1522613_+|outer-membrane-protein
MKKFFSQSLLALIVSMNALLAMDGNGVFLGAGYLQGQAQMHADINSQKQATNATIKGFDALLGYQFFFEKHFGLRLYGFFDYAHANSIRLKNPNYNNEVAQLAGQILGKQEINRLTSLADPKTFEPNMLTYGGAMDVMVNVINNGIMSLGAFGGVQLAGNSWLMATPSFEGILVEQALVSKKATSFQFLFNVGARLRILKHSSIEAGVKFPMLKKNPYITAKNLDIGFRRVYSWYVNYVFTF
>NZ_CP032479.1|WP_139543514.1|1522623_1524384_-|DUF262-domain-containing-protein
MAKIESNDLNLRDILKDELYYQIPIYQRPYQWTEENCEKLLDDLFFIIEDDRESDYFCDSLVLILISEDPKKAKTYDVVDGQQRLSTFILLTKVLATLYKNNEVLNDKTSRDFLEKSLGDTDGEKRKRLTFNTIGLNAKDDFQDALDFFDNSHASKNNKNNYLKNAVCLKNYLKEKEIENINAFIKWLYFKVVFIKTTCPNISMALRIFSVLNARGLPLHAIDIFKAELLKKLANKKDQEEFVFRWNALRQKCSKNESKFPKRKESKREKNAAEILFSWYLAYLNPVTSKEKMEKRLVTWFKNLNKTPLEYFKGVEDFYNAYCEVLEMQDRHAYLLSYKDDDHLCVMLCTSLLHHYSDQEIKALKELLVKFYYQGWVAEKTKSIRSQTCCNIINALKEKQSVENIASIMKKYFKDKNITQRFKENLQDSNLYTKFYYTGKTPKKNSWLKPILILVNYFMSDDANPAYIKMDDDFHVEHILPQKPDSSSQWVKDFSEEERELYTHSLANLTLLGGTKNSQASNLDFKDKKKIYMGEENRLRKTKTSKVMTCYKMTIDIAHHYTEWTPKSLEKRKEELIQKIESVLVL
>NZ_CP032479.1|WP_139543515.1|1524515_1525613_-|alcohol-dehydrogenase-catalytic-domain-containing-protein
MHNACNCNNQRVPFKGFAIDNKDGKFHEWEHSRHAMGEYDVVIDTHFSGICHTDIHFAHSDWLPGIYPMVPGHEIAGVVSAVGSKVTKFKVGDHAGVGCMVNSCGECHTCKHEHQEQWCENNKTIYTYSWEDSFHNNEPTYGGYSNNIVVSENFVISIPKEAPLDKAAPLLCAGITVYSPLKFSKVTKGSKVGIAGFGGLGHMALKYAVAMGAQVSVFARNDKKKQMAQKLGATNFYTSVEECKEKFDLIISSIPTQYDLLAYTKLLKYGGEVAIVGIPPKDPQRNLDFGDLVLFSGNHKVYGSWIGGVKETQEMMDFSIKHGIYPEIELVTGQEIDTTWDKLLNGQGNFRYVIDMKKSMENFKK
>NZ_CP032479.1|WP_139543516.1|1525882_1527025_-|NAD-binding-protein
MTIGLVKESMDLESRVALVPDDVALIVQKGVGVLVENHAGANSGYSNEAYESVGAKIVDTKTAWGQDLVVKCKEPLEHEYPLLKEKATLFSYLDLAYQKSLCEMFIDKKITSICTETIAGPKNDYPILAPMSVVAGRLAAHLIQHYLLALKHVKGFMGKGVMLGGLSGAQRAKIVVVGGGVVGMESAKVLSQMGAKVTILELDYAKLQNHPYYHLYDLEVLSVNEANIIQALSGAVGLVGAVLVTASQTPKVILRRHLKYMQKQGVVVDVACDLGGCIETIHQTSHSNPVYVEEDLLHYGVPNMPGIVAKTSSVAYSHASAPYLLYYLEHGLKGFLTANTKIVANTLGGLSAYNGYIAQEGIAKAFNLAFKSPLEVLKEL
>NZ_CP032479.1|WP_139543517.1|1527228_1528197_+|arginase
MILVGLEAELGASKRGTDKGVRRLREALSKTHGDVIKGMQTITQERCVLYKEFRYAKNFEDYYLFCKENLIPCMKEVFEKKEFPLILSSEHANMFGIFQAFRSVHKDKKIGILYLDAHADIHTAYDSDSKHIHGMPLGMVLNRVRSGFNRMSESEEKAWQKLCSLGLEKGGLEIDPKCLVYFGVRSTEKSERDVIKELEIPLFSVDAIRENMQEVVQKTKESLKAVDIIYLSLDLDIMDGKLFTSTGVRENNGLSFDELKQLLGLLLENFKDRLKAVEVTEYNPTVSTKHNNEEEKQVLEILDLIINRCKIKDKKRSFARSY
>NZ_CP032479.1|WP_139543518.1|1528552_1531078_+|TonB-dependent-receptor-plug-domain-containing-protein
MHKKVLLALTASLICQESLFSKEKDYTLGKVSTAGKKDRSDYSGQVNLGYSGITAPKSWQDEEVKKYTGSRTVISNKALTQQANQSIEEALQNVPGLQIRNATGVGAMPTIQIRGFGAGGSGHSDATLMLVNGIPVYMAPYAHIELDIFPVTFQAIDRIDVIKGGGSVQYGPNTYGGIVNIITKPIPTQWENQAAERITYWAKARNAGFAAPPDKTGDPSFIKSLGNNLLYNTYVRSGGMINKHVGIQAQANWVRGQGFRDNSPSNISNYWLDGIYDINENNGIKAYYQYYDFAIAQPGSLSEQDYKINRFANLRPLNQKGGRSQRFGAVYENRFGDLDKVGGTFSFTYYGQLMTRDFQVSSSYNSANMVTCFSEAACRAAGLPAGYNLAVPYYATNYNGWAEVENPVRSINNAFEPKVNLIVNTGKVKQTFIMGLRFMTTTFLQRQYLNTNECATKTSGEGAGFLCEGANVMSGWKPHIKHGVYRNWNNWRNNYTAVYLSDRIEAWDGRFFIVPGLRYAFVQYNNENAANWMQIPEKDLRKIKHMNNWMPSTNIGFIPVQGDHNVLTYFNYQRSFVPPQLDVLSYGGAEYFTQHFDTVEAGARYTYKDKFSFNADYFRIWARDFATGQYSVYTSGPMKGNVRPINGYSQGVELELYYRPIRGLQFHAAFNYIDTRVTSHGPLTDLNGDVLKGTSYNKHFPFVSPFQFIFDARYNWRKTTIGISSYFYSRAYSGISNSAAGGYYGMQYYSGGNNYESVLNSGYQCEAWCMTQHEGLLPWYWVWNIQVSQIFWENGRHRVTGSLQINNIFNMKYYFTGIGSSPAGLQPAPGRSVTAYLNYTF

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity
NZ_CP032479_1	1.3|351025|36|NZ_CP032479|CRISPRCasFinder	351025-351060	36	KX119177	Helicobacter phage SwA626G, complete genome	14992-15027	2	0.944
NZ_CP032479_1	1.3|351025|36|NZ_CP032479|CRISPRCasFinder	351025-351060	36	KX119177	Helicobacter phage SwA626G, complete genome	15058-15093	3	0.917
NZ_CP032479_1	1.1|350893|36|NZ_CP032479|CRISPRCasFinder	350893-350928	36	KX119206	Helicobacter phage UKEN32U, complete genome	14386-14421	4	0.889
NZ_CP032479_1	1.1|350893|36|NZ_CP032479|CRISPRCasFinder	350893-350928	36	KX119174	Helicobacter phage UKEN31U, complete genome	14386-14421	4	0.889
NZ_CP032479_1	1.1|350893|36|NZ_CP032479|CRISPRCasFinder	350893-350928	36	KX119199	Helicobacter phage Pt5771G, complete genome	13859-13894	5	0.861
NZ_CP032479_2	2.1|548346|31|NZ_CP032479|CRISPRCasFinder	548346-548376	31	NZ_LR214986	Mycoplasma cynos strain NCTC10142 plasmid 13	748932-748962	6	0.806
NZ_CP032479_2	2.1|548346|31|NZ_CP032479|CRISPRCasFinder	548346-548376	31	NZ_LR214986	Mycoplasma cynos strain NCTC10142 plasmid 13	725202-725232	7	0.774
NZ_CP032479_2	2.1|548346|31|NZ_CP032479|CRISPRCasFinder	548346-548376	31	KP682381	Escherichia phage PA28, complete genome	11580-11610	8	0.742
NZ_CP032479_2	2.1|548346|31|NZ_CP032479|CRISPRCasFinder	548346-548376	31	MH937459	Streptococcus phage CHPC595, complete genome	7992-8022	8	0.742
NZ_CP032479_2	2.1|548346|31|NZ_CP032479|CRISPRCasFinder	548346-548376	31	MF580769	Streptococcus phage L5A1, complete genome	7950-7980	8	0.742
NZ_CP032479_2	2.1|548346|31|NZ_CP032479|CRISPRCasFinder	548346-548376	31	MH937458	Streptococcus phage CHPC572, complete genome	8032-8062	8	0.742
NZ_CP032479_2	2.1|548346|31|NZ_CP032479|CRISPRCasFinder	548346-548376	31	MH937483	Streptococcus phage CHPC1005, complete genome	9418-9448	8	0.742
NZ_CP032479_2	2.1|548346|31|NZ_CP032479|CRISPRCasFinder	548346-548376	31	MF580773	Streptococcus phage STP1, complete genome	7961-7991	8	0.742
NZ_CP032479_2	2.1|548346|31|NZ_CP032479|CRISPRCasFinder	548346-548376	31	MH892359	Streptococcus phage SW10, complete genome	8877-8907	8	0.742
NZ_CP032479_2	2.1|548346|31|NZ_CP032479|CRISPRCasFinder	548346-548376	31	KY421186	Flavobacterium phage FL-1, complete genome	5706-5736	8	0.742
NZ_CP032479_2	2.1|548346|31|NZ_CP032479|CRISPRCasFinder	548346-548376	31	MF580766	Streptococcus phage B0, complete genome	7961-7991	8	0.742
NZ_CP032479_2	2.1|548346|31|NZ_CP032479|CRISPRCasFinder	548346-548376	31	NC_002072	Streptococcus phage DT1, complete genome	8510-8540	8	0.742
NZ_CP032479_2	2.1|548346|31|NZ_CP032479|CRISPRCasFinder	548346-548376	31	AF085222	Streptococcus thermophilus bacteriophage DT1, complete genome	8510-8540	8	0.742
NZ_CP032479_2	2.1|548346|31|NZ_CP032479|CRISPRCasFinder	548346-548376	31	NC_013645	Streptococcus phage Abc2, complete genome	7962-7992	8	0.742
NZ_CP032479_2	2.1|548346|31|NZ_CP032479|CRISPRCasFinder	548346-548376	31	MF580774	Streptococcus phage STP2, complete genome	7949-7979	8	0.742
NZ_CP032479_2	2.1|548346|31|NZ_CP032479|CRISPRCasFinder	548346-548376	31	MF580767	Streptococcus phage B5, complete genome	8202-8232	8	0.742
NZ_CP032479_2	2.1|548346|31|NZ_CP032479|CRISPRCasFinder	548346-548376	31	NC_018989	Borrelia burgdorferi 297 plasmid 297_lp28-5, complete sequence	14226-14256	8	0.742
NZ_CP032479_2	2.1|548346|31|NZ_CP032479|CRISPRCasFinder	548346-548376	31	NC_015704	Runella slithyformis DSM 19594 plasmid pRUNSL02, complete sequence	47820-47850	8	0.742
NZ_CP032479_2	2.1|548346|31|NZ_CP032479|CRISPRCasFinder	548346-548376	31	GQ866233	Aggregatibacter phage S1249, complete sequence	29180-29210	8	0.742
NZ_CP032479_2	2.1|548346|31|NZ_CP032479|CRISPRCasFinder	548346-548376	31	AP013419	Uncultured Mediterranean phage uvMED DNA, complete genome, group G16, isolate: uvMED-CGR-C48B-MedDCM-OCT-S41-C26	19904-19934	9	0.71
NZ_CP032479_2	2.1|548346|31|NZ_CP032479|CRISPRCasFinder	548346-548376	31	AP013416	Uncultured Mediterranean phage uvMED DNA, complete genome, group G16, isolate: uvMED-CGR-C48B-MedDCM-OCT-S24-C17	31226-31256	9	0.71
NZ_CP032479_2	2.1|548346|31|NZ_CP032479|CRISPRCasFinder	548346-548376	31	AP013417	Uncultured Mediterranean phage uvMED DNA, complete genome, group G16, isolate: uvMED-CGR-C48B-MedDCM-OCT-S26-C25	37347-37377	9	0.71
NZ_CP032479_2	2.1|548346|31|NZ_CP032479|CRISPRCasFinder	548346-548376	31	MK765564	Tortoise microvirus 14 isolate 14_SP_70, complete genome	3229-3259	9	0.71
NZ_CP032479_2	2.1|548346|31|NZ_CP032479|CRISPRCasFinder	548346-548376	31	MT813197	Escherichia phage vB_EcoP_Kapi1, complete genome	6872-6902	10	0.677

1. spacer 1.3|351025|36|NZ_CP032479|CRISPRCasFinder matches to KX119177 (Helicobacter phage SwA626G, complete genome) position: , mismatch: 2, identity: 0.944

cgaaagccttgaagcgatcacagaagcgaaaacgaa	CRISPR spacer
cgaaagccttgaagcgatcacgcaagcgaaaacgaa	Protospacer
*********************. *************

2. spacer 1.3|351025|36|NZ_CP032479|CRISPRCasFinder matches to KX119177 (Helicobacter phage SwA626G, complete genome) position: , mismatch: 3, identity: 0.917

cgaaagccttgaagcgatcacagaagcgaaaacgaa	CRISPR spacer
cgaaagccttgaagcgatcacgcaagcgaaaacgag	Protospacer
*********************. ************.

3. spacer 1.1|350893|36|NZ_CP032479|CRISPRCasFinder matches to KX119206 (Helicobacter phage UKEN32U, complete genome) position: , mismatch: 4, identity: 0.889

ccaaagccttgaagcgattacacttgctaaaacaac	CRISPR spacer
cgaaagccttgaagcgataacacttgctaaaacgaa	Protospacer
* **************** **************.* 

4. spacer 1.1|350893|36|NZ_CP032479|CRISPRCasFinder matches to KX119174 (Helicobacter phage UKEN31U, complete genome) position: , mismatch: 4, identity: 0.889

ccaaagccttgaagcgattacacttgctaaaacaac	CRISPR spacer
cgaaagccttgaagcgataacacttgctaaaacgaa	Protospacer
* **************** **************.* 

5. spacer 1.1|350893|36|NZ_CP032479|CRISPRCasFinder matches to KX119199 (Helicobacter phage Pt5771G, complete genome) position: , mismatch: 5, identity: 0.861

ccaaagccttgaagcgattacacttgctaaaacaac	CRISPR spacer
cgcaagccttgaagcgattacgcttgctaaaacgaa	Protospacer
*  ******************.***********.* 

6. spacer 2.1|548346|31|NZ_CP032479|CRISPRCasFinder matches to NZ_LR214986 (Mycoplasma cynos strain NCTC10142 plasmid 13) position: , mismatch: 6, identity: 0.806

atttttgatctttaattttttcactgatttt---	CRISPR spacer
ttttttgatttttaattttttaac---ttttaga	Protospacer
 ********.*********** **   ****   

7. spacer 2.1|548346|31|NZ_CP032479|CRISPRCasFinder matches to NZ_LR214986 (Mycoplasma cynos strain NCTC10142 plasmid 13) position: , mismatch: 7, identity: 0.774

atttttgatctttaattttttcactgatttt	CRISPR spacer
cttcttgatatttaattttttcacctttttc	Protospacer
 **.***** **************.  ***.

8. spacer 2.1|548346|31|NZ_CP032479|CRISPRCasFinder matches to KP682381 (Escherichia phage PA28, complete genome) position: , mismatch: 8, identity: 0.742

atttttgatctttaattttttcactgatttt	CRISPR spacer
atttttcatctttaattttttcaacatctcg	Protospacer
****** **************** .. .*. 

9. spacer 2.1|548346|31|NZ_CP032479|CRISPRCasFinder matches to MH937459 (Streptococcus phage CHPC595, complete genome) position: , mismatch: 8, identity: 0.742

atttttgatctttaattttttcactgatttt	CRISPR spacer
tgtccccatcttcaattttgtcactgatttt	Protospacer
  *... *****.****** ***********

10. spacer 2.1|548346|31|NZ_CP032479|CRISPRCasFinder matches to MF580769 (Streptococcus phage L5A1, complete genome) position: , mismatch: 8, identity: 0.742

atttttgatctttaattttttcactgatttt	CRISPR spacer
tgtccccatcttcaaatttttcactgatttt	Protospacer
  *... *****.** ***************

11. spacer 2.1|548346|31|NZ_CP032479|CRISPRCasFinder matches to MH937458 (Streptococcus phage CHPC572, complete genome) position: , mismatch: 8, identity: 0.742

atttttgatctttaattttttcactgatttt	CRISPR spacer
tgtccccatcttcaattttgtcactgatttt	Protospacer
  *... *****.****** ***********

12. spacer 2.1|548346|31|NZ_CP032479|CRISPRCasFinder matches to MH937483 (Streptococcus phage CHPC1005, complete genome) position: , mismatch: 8, identity: 0.742

atttttgatctttaattttttcactgatttt	CRISPR spacer
tgtccccatcttcaattttgtcactgatttt	Protospacer
  *... *****.****** ***********

13. spacer 2.1|548346|31|NZ_CP032479|CRISPRCasFinder matches to MF580773 (Streptococcus phage STP1, complete genome) position: , mismatch: 8, identity: 0.742

atttttgatctttaattttttcactgatttt	CRISPR spacer
tgtccccatcttcaaatttttcactgatttt	Protospacer
  *... *****.** ***************

14. spacer 2.1|548346|31|NZ_CP032479|CRISPRCasFinder matches to MH892359 (Streptococcus phage SW10, complete genome) position: , mismatch: 8, identity: 0.742

atttttgatctttaattttttcactgatttt	CRISPR spacer
tgtccccatcttcaaatttttcactgatttt	Protospacer
  *... *****.** ***************

15. spacer 2.1|548346|31|NZ_CP032479|CRISPRCasFinder matches to KY421186 (Flavobacterium phage FL-1, complete genome) position: , mismatch: 8, identity: 0.742

atttttgatctttaattttttcactgatttt	CRISPR spacer
attttccatctttaattttttcacatctgcc	Protospacer
*****. *****************   * ..

16. spacer 2.1|548346|31|NZ_CP032479|CRISPRCasFinder matches to MF580766 (Streptococcus phage B0, complete genome) position: , mismatch: 8, identity: 0.742

atttttgatctttaattttttcactgatttt	CRISPR spacer
tgtccccatcttcaaatttttcactgatttt	Protospacer
  *... *****.** ***************

17. spacer 2.1|548346|31|NZ_CP032479|CRISPRCasFinder matches to NC_002072 (Streptococcus phage DT1, complete genome) position: , mismatch: 8, identity: 0.742

atttttgatctttaattttttcactgatttt	CRISPR spacer
tgtccccatcttcaattttgtcactgatttt	Protospacer
  *... *****.****** ***********

18. spacer 2.1|548346|31|NZ_CP032479|CRISPRCasFinder matches to AF085222 (Streptococcus thermophilus bacteriophage DT1, complete genome) position: , mismatch: 8, identity: 0.742

atttttgatctttaattttttcactgatttt	CRISPR spacer
tgtccccatcttcaattttgtcactgatttt	Protospacer
  *... *****.****** ***********

19. spacer 2.1|548346|31|NZ_CP032479|CRISPRCasFinder matches to NC_013645 (Streptococcus phage Abc2, complete genome) position: , mismatch: 8, identity: 0.742

atttttgatctttaattttttcactgatttt	CRISPR spacer
tgtccccatcttcaattttgtcactgatttt	Protospacer
  *... *****.****** ***********

20. spacer 2.1|548346|31|NZ_CP032479|CRISPRCasFinder matches to MF580774 (Streptococcus phage STP2, complete genome) position: , mismatch: 8, identity: 0.742

atttttgatctttaattttttcactgatttt	CRISPR spacer
tgtccccatcttcaaatttttcactgatttt	Protospacer
  *... *****.** ***************

21. spacer 2.1|548346|31|NZ_CP032479|CRISPRCasFinder matches to MF580767 (Streptococcus phage B5, complete genome) position: , mismatch: 8, identity: 0.742

atttttgatctttaattttttcactgatttt	CRISPR spacer
tgtccccatcttcaaatttttcactgatttt	Protospacer
  *... *****.** ***************

22. spacer 2.1|548346|31|NZ_CP032479|CRISPRCasFinder matches to NC_018989 (Borrelia burgdorferi 297 plasmid 297_lp28-5, complete sequence) position: , mismatch: 8, identity: 0.742

atttttgatctttaattttttcactgatttt	CRISPR spacer
attttttatgtttaattttttcatatgcttg	Protospacer
****** ** *************.  ..** 

23. spacer 2.1|548346|31|NZ_CP032479|CRISPRCasFinder matches to NC_015704 (Runella slithyformis DSM 19594 plasmid pRUNSL02, complete sequence) position: , mismatch: 8, identity: 0.742

atttttgatctttaattttttcactgatttt	CRISPR spacer
tttcggcatcttttagtttttcactgatttg	Protospacer
 **.   ****** * ************** 

24. spacer 2.1|548346|31|NZ_CP032479|CRISPRCasFinder matches to GQ866233 (Aggregatibacter phage S1249, complete sequence) position: , mismatch: 8, identity: 0.742

atttttgatctttaattttttcactgatttt	CRISPR spacer
atttttgatctgtcattttttcattcctaaa	Protospacer
*********** * *********.*  *   

25. spacer 2.1|548346|31|NZ_CP032479|CRISPRCasFinder matches to AP013419 (Uncultured Mediterranean phage uvMED DNA, complete genome, group G16, isolate: uvMED-CGR-C48B-MedDCM-OCT-S41-C26) position: , mismatch: 9, identity: 0.71

atttttgatctttaattttttcactgatttt	CRISPR spacer
ctaacccttcttttatttttttactgatttt	Protospacer
 *  ..  ***** *******.*********

26. spacer 2.1|548346|31|NZ_CP032479|CRISPRCasFinder matches to AP013416 (Uncultured Mediterranean phage uvMED DNA, complete genome, group G16, isolate: uvMED-CGR-C48B-MedDCM-OCT-S24-C17) position: , mismatch: 9, identity: 0.71

atttttgatctttaattttttcactgatttt	CRISPR spacer
ctaacccttcttttatttttttactgatttt	Protospacer
 *  ..  ***** *******.*********

27. spacer 2.1|548346|31|NZ_CP032479|CRISPRCasFinder matches to AP013417 (Uncultured Mediterranean phage uvMED DNA, complete genome, group G16, isolate: uvMED-CGR-C48B-MedDCM-OCT-S26-C25) position: , mismatch: 9, identity: 0.71

atttttgatctttaattttttcactgatttt	CRISPR spacer
ctaacccttcttttatttttttactgatttt	Protospacer
 *  ..  ***** *******.*********

28. spacer 2.1|548346|31|NZ_CP032479|CRISPRCasFinder matches to MK765564 (Tortoise microvirus 14 isolate 14_SP_70, complete genome) position: , mismatch: 9, identity: 0.71

atttttgatctttaattttttcactgatttt	CRISPR spacer
atttttgatttttaattgtttcaagtaaaga	Protospacer
*********.******* *****   *    

29. spacer 2.1|548346|31|NZ_CP032479|CRISPRCasFinder matches to MT813197 (Escherichia phage vB_EcoP_Kapi1, complete genome) position: , mismatch: 10, identity: 0.677

atttttgatctttaattttttcactgatttt	CRISPR spacer
atttttcatcttcaattttttcaacatcgcg	Protospacer
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