CRISPRimmunity

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Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Self targeting spacer number	Target MGE spacer number	Prophage number	Anti-CRISPR protein number
NZ_CP041357	Bacillus halotolerans strain F41-3 chromosome, complete genome	2 crisprs	csa3,cas3,DEDDh,WYL,DinG	0	2	7	0

Results visualization

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CRISPR-Cas detection and classification

Crispr_ID: NZ_CP041357_1

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

NZ_CP041357_1

455505-455609

Orphan

Consensus_repeat	Method
TGATGGGAATCGAACCCACGACAT	CRISPRCasFinder

1 spacers

The CRISPR arrays of NZ_CP041357_1

>merge|NZ_CP041357|1|455505-455609|CRISPRCasFinder
TGATGGGAATCGAACCCACGACATCAGCTTGGAAGGCTGAGGTTTTACCACTAAACTACACCCGCAATTTTTTGGGGCGATTGATGGGAATCGAACCCACGAATG

>NZ_CP041357|1|1|455505-455609|CRISPRCasFinder
TGATGGGAATCGAACCCACGACAT	CAGCTTGGAAGGCTGAGGTTTTACCACTAAACTACACCCGCAATTTTTTGGGGCGAT
TGATGGGAATCGAACCCACGAATG

Protein	Signature genes	Signature genes Name	Protein_function
NZ_CP041357.1\|WP_151174471.1\|468399_469872_-\|betaine-aldehyde-dehydrogenase	unknown	unknown	gnl\|CDD\|143437
NZ_CP041357.1\|WP_101860056.1\|465321_465846_-\|hypothetical-protein	unknown	unknown	gnl\|CDD\|376684
NZ_CP041357.1\|WP_095714592.1\|450825_451857_-\|glucose-1-phosphate-adenylyltransferase-subunit-GlgD	unknown	unknown	gnl\|CDD\|273966
NZ_CP041357.1\|WP_106019451.1\|470072_470615_+\|GbsR/MarR-family-transcriptional-regulator	unknown	unknown	gnl\|CDD\|224427
NZ_CP041357.1\|WP_151174464.1\|446493_446961_+\|tryptophan-rich-sensory-protein	unknown	unknown	gnl\|CDD\|226007
NZ_CP041357.1\|WP_024122606.1\|445621_446341_+\|yteA-family-sporulation-protein	unknown	unknown	gnl\|CDD\|274340
NZ_CP041357.1\|WP_101863550.1\|442175_443918_-\|2-succinyl-5-enolpyruvyl-6-hydroxy-3--cyclohexene-1-carboxylic-acid-synthase	unknown	unknown	gnl\|CDD\|224087
NZ_CP041357.1\|WP_059292679.1\|463773_465303_-\|flotillin-lipid-rafts-scaffold-protein-FloT	unknown	unknown	gnl\|CDD\|225177
NZ_CP041357.1\|WP_151174465.1\|446991_449388_-\|glycogen/starch/alpha-glucan-family-phosphorylase	unknown	unknown	gnl\|CDD\|340853
NZ_CP041357.1\|WP_127696596.1\|466506_467085_-\|MOSC-domain-containing-protein	unknown	unknown	gnl\|CDD\|377051
NZ_CP041357.1\|WP_151174470.1\|466012_466501_+\|DUF1569-domain-containing-protein	unknown	unknown	gnl\|CDD\|378981
NZ_CP041357.1\|WP_151174469.1\|463222_463747_-\|GNAT-family-N-acetyltransferase	unknown	unknown	gnl\|CDD\|224168
NZ_CP041357.1\|WP_151174467.1\|451881_453024_-\|glucose-1-phosphate-adenylyltransferase	unknown	unknown	gnl\|CDD\|179997
NZ_CP041357.1\|WP_101864219.1\|470822_471368_+\|biofilm-surface-layer-protein-BslA	unknown	unknown	gnl\|CDD\|379999
NZ_CP041357.1\|WP_151174466.1\|449374_450829_-\|glycogen-synthase-GlgA	unknown	unknown	gnl\|CDD\|234809
NZ_CP041357.1\|WP_024122603.1\|441363_442188_-\|2-succinyl-6-hydroxy-2,-4-cyclohexadiene-1-carboxylate-synthase	unknown	unknown	gnl\|CDD\|274729
NZ_CP041357.1\|WP_151174468.1\|453020_454904_-\|1,4-alpha-glucan-branching-enzyme	unknown	unknown	gnl\|CDD\|273667
NZ_CP041357.1\|WP_024122615.1\|467174_468383_-\|choline-dehydrogenase	unknown	unknown	gnl\|CDD\|341481
NZ_CP041357.1\|WP_024122619.1\|471747_472416_+\|TrkA-family-potassium-uptake-protein	unknown	unknown	gnl\|CDD\|223643
NZ_CP041357.1\|WP_106020318.1\|443914_445330_-\|isochorismate-synthase-MenF	unknown	unknown	gnl\|CDD\|224091

Protein	Function_ID	Function_description	E-value
NZ_CP041357.1\|WP_151174471.1\|468399_469872_-\|betaine-aldehyde-dehydrogenase	gnl\|CDD\|143437	cd07119, ALDH_BADH-GbsA, Bacillus subtilis NAD+-dependent betaine aldehyde dehydrogenase-like. Included in this CD is the NAD+-dependent, betaine aldehyde dehydrogenase (BADH, GbsA, EC=1.2.1.8) of Bacillus subtilis involved in the synthesis of the osmoprotectant glycine betaine from choline or glycine betaine aldehyde.	0
NZ_CP041357.1\|WP_101860056.1\|465321_465846_-\|hypothetical-protein	gnl\|CDD\|376684	pfam01957, NfeD, NfeD-like C-terminal, partner-binding. NfeD-like proteins are widely distributed throughout prokaryotes and are frequently associated with genes encoding stomatin-like proteins (slipins). There appear to be three major groups: an ancestral group with only an N-terminal serine protease domain and this C-terminal beta sheet-rich domain which is structurally very similar to the OB-fold domain, associated with its neighboring slipin cluster; a second major group with an additional middle, membrane-spanning domain, associated in some species with eoslipin and in others with yqfA; a final 'artificial' group which unites truncated forms lacking the protease region and associated with their ancestral gene partner, either yqfA or eoslipin. This NefD, C-terminal, domain appears to be the major one for relating to the associated protein. NfeD homologs are clearly reliant on their conserved gene neighbor which is assumed to be necessary for function, either through direct physical interaction or by functioning in the same pathway, possibly involve with lipid-rafts.	5.92482e-06
NZ_CP041357.1\|WP_095714592.1\|450825_451857_-\|glucose-1-phosphate-adenylyltransferase-subunit-GlgD	gnl\|CDD\|273966	TIGR02092, Glycogen_biosynthesis_protein_GlgD, glucose-1-phosphate adenylyltransferase, GlgD subunit. This family is GlgD, an apparent regulatory protein that appears in an alpha2/beta2 heterotetramer with GlgC (glucose-1-phosphate adenylyltransferase, TIGR02091) in a subset of bacteria that use GlgC for glycogen biosynthesis. [Energy metabolism, Biosynthesis and degradation of polysaccharides].	0
NZ_CP041357.1\|WP_106019451.1\|470072_470615_+\|GbsR/MarR-family-transcriptional-regulator	gnl\|CDD\|224427	COG1510, COG1510, Predicted transcriptional regulators [Transcription].	7.44578e-69
NZ_CP041357.1\|WP_151174464.1\|446493_446961_+\|tryptophan-rich-sensory-protein	gnl\|CDD\|226007	COG3476, COG3476, Tryptophan-rich sensory protein (mitochondrial benzodiazepine receptor homolog) [Signal transduction mechanisms].	4.00179e-35
NZ_CP041357.1\|WP_024122606.1\|445621_446341_+\|yteA-family-sporulation-protein	gnl\|CDD\|274340	TIGR02890, conserved_hypothetical_protein, regulatory protein, yteA family. Members of this predicted regulatory protein are found only in endospore-forming members of the Firmicutes group of bacteria, and in nearly every such species; Clostridium perfringens seems to be an exception. The member from Bacillus subtilis, the model system for the study of the sporulation program, has been designated both yteA and yzwB. Some (but not all) members of this family show a strong sequence match to Pfam family pfam01258 the C4-type zinc finger protein, DksA/TraR family, but only one of the four key Cys residues is conserved. All members of this protein family share an additional C-terminal domain. Smaller proteins from the proteobacteria with just the N-terminal domain, including DksA and DksA2 are RNA polymerase-binding regulatory proteins even if the Zn-binding site is not conserved. [Unknown function, General].	1.62617e-67
NZ_CP041357.1\|WP_101863550.1\|442175_443918_-\|2-succinyl-5-enolpyruvyl-6-hydroxy-3--cyclohexene-1-carboxylic-acid-synthase	gnl\|CDD\|224087	COG1165, MenD, 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase [Coenzyme metabolism].	0
NZ_CP041357.1\|WP_059292679.1\|463773_465303_-\|flotillin-lipid-rafts-scaffold-protein-FloT	gnl\|CDD\|225177	COG2268, COG2268, Uncharacterized protein conserved in bacteria [Function unknown].	1.01845e-119
NZ_CP041357.1\|WP_151174465.1\|446991_449388_-\|glycogen/starch/alpha-glucan-family-phosphorylase	gnl\|CDD\|340853	cd04300, GT35_Glycogen_Phosphorylase, glycogen phosphorylase and similar proteins. This is a family of oligosaccharide phosphorylases. It includes yeast and mammalian glycogen phosphorylases, plant starch/glucan phosphorylase, as well as the maltodextrin phosphorylases of bacteria. The members of this family catalyze the breakdown of oligosaccharides into glucose-1-phosphate units. They are important allosteric enzymes in carbohydrate metabolism. The allosteric control mechanisms of yeast and mammalian members of this family are different from that of bacterial members. The members of this family belong to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility.	0
NZ_CP041357.1\|WP_127696596.1\|466506_467085_-\|MOSC-domain-containing-protein	gnl\|CDD\|377051	pfam03473, MOSC, MOSC domain. The MOSC (MOCO sulfurase C-terminal) domain is a superfamily of beta-strand-rich domains identified in the molybdenum cofactor sulfurase and several other proteins from both prokaryotes and eukaryotes. These MOSC domains contain an absolutely conserved cysteine and occur either as stand-alone forms or fused to other domains such as NifS-like catalytic domain in Molybdenum cofactor sulfurase. The MOSC domain is predicted to be a sulfur-carrier domain that receives sulfur abstracted by the pyridoxal phosphate-dependent NifS-like enzymes, on its conserved cysteine, and delivers it for the formation of diverse sulfur-metal clusters.	2.3422e-06
NZ_CP041357.1\|WP_151174470.1\|466012_466501_+\|DUF1569-domain-containing-protein	gnl\|CDD\|378981	pfam12867, DinB_2, DinB superfamily. The DinB family are an uncharacterized family of potential enzymes. The structure of these proteins is composed of a four helix bundle.	5.31505e-18
NZ_CP041357.1\|WP_151174469.1\|463222_463747_-\|GNAT-family-N-acetyltransferase	gnl\|CDD\|224168	COG1247, COG1247, Sortase and related acyltransferases [Cell envelope biogenesis, outer membrane].	5.09242e-11
NZ_CP041357.1\|WP_151174467.1\|451881_453024_-\|glucose-1-phosphate-adenylyltransferase	gnl\|CDD\|179997	PRK05293, glgC, glucose-1-phosphate adenylyltransferase; Provisional.	0
NZ_CP041357.1\|WP_101864219.1\|470822_471368_+\|biofilm-surface-layer-protein-BslA	gnl\|CDD\|379999	pfam17735, BslA, Biofilm surface layer A. This family includes members such as BslA (previously called YuaB). Secreted BslA from Bacillus subtillis has been shown to form surface layers around the biofilm self-assembling at interfaces of B. subtilis biofilms, forming an elastic film. structural analysis revealed that BslA consists of an Ig-type fold with the addition of an unusual, extremely hydrophobic cap region. The hydrophobic cap exhibits physiochemical properties similar to the hydrophobic surface found in fungal hydrophobins; thus, BslA is defined as member of a class of bacterially produced hydrophobins.	8.65828e-48
NZ_CP041357.1\|WP_151174466.1\|449374_450829_-\|glycogen-synthase-GlgA	gnl\|CDD\|234809	PRK00654, glgA, glycogen synthase GlgA.	0
NZ_CP041357.1\|WP_024122603.1\|441363_442188_-\|2-succinyl-6-hydroxy-2,-4-cyclohexadiene-1-carboxylate-synthase	gnl\|CDD\|274729	TIGR03695, menH_SHCHC, 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase. This protein catalyzes the formation of SHCHC, or (1 R,6 R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate, by elmination of pyruvate from 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC). Note that SHCHC synthase activity previously was attributed to MenD, which in fact is SEPHCHC synthase. [Biosynthesis of cofactors, prosthetic groups, and carriers, Menaquinone and ubiquinone].	2.65666e-122
NZ_CP041357.1\|WP_151174468.1\|453020_454904_-\|1,4-alpha-glucan-branching-enzyme	gnl\|CDD\|273667	TIGR01515, branching_enzym, alpha-1,4-glucan:alpha-1,4-glucan 6-glycosyltransferase. This model describes the glycogen branching enzymes which are responsible for the transfer of chains of approx. 7 alpha(1--4)-linked glucosyl residues to other similar chains (in new alpha(1--6) linkages) in the biosynthesis of glycogen. This enzyme is a member of the broader amylase family of starch hydrolases which fold as (beta/alpha)8 barrels, the so-called TIM-barrel structure. All of the sequences comprising the seed of this model have been experimentally characterized. This model encompasses both bacterial and eukaryotic species. No archaea have this enzyme, although Aquifex aolicus does. Two species, Bacillus thuringiensis and Clostridium perfringens have two sequences each which are annotated as amylases. These annotations are aparrently in error. GP\|18143720 from C. perfringens, for instance, contains the note "674 aa, similar to gp:A14658_1 amylase (1,4-alpha-glucan branching enzyme (EC 2.4.1.18) ) from Bacillus thuringiensis (648 aa); 51.1% identity in 632 aa overlap." A branching enzyme from Porphyromonas gingivales, OMNI\|PG1793, appears to be more closely related to the eukaryotic species (across a deep phylogenetic split) and may represent an instance of lateral transfer from this species' host. A sequence from Arabidopsis thaliana, GP\|9294564, scores just above trusted, but appears either to contain corrupt sequence or, more likely, to be a pseudogene as some of the conserved catalytic residues common to the alpha amylase family are not conserved here. [Energy metabolism, Biosynthesis and degradation of polysaccharides].	0
NZ_CP041357.1\|WP_024122615.1\|467174_468383_-\|choline-dehydrogenase	gnl\|CDD\|341481	cd08551, Fe-ADH, iron-containing alcohol dehydrogenases (Fe-ADH)-like. This family contains large metal-containing alcohol dehydrogenases (ADH), known as iron-containing alcohol dehydrogenases. They contain a dehydroquinate synthase-like protein structural fold and mostly contain iron. They are distinct from other alcohol dehydrogenases which contains different protein domains. There are several distinct families of alcohol dehydrogenases: Zinc-containing long-chain alcohol dehydrogenases, insect-type, or short-chain alcohol dehydrogenases, iron-containing alcohol dehydrogenases, among others. The iron-containing family has a Rossmann fold-like topology that resembles the fold of the zinc-dependent alcohol dehydrogenases, but lacks sequence homology, and differs in strand arrangement. ADH catalyzes the reversible oxidation of alcohol to acetaldehyde with the simultaneous reduction of NAD(P)+ to NAD(P)H.	6.23242e-174
NZ_CP041357.1\|WP_024122619.1\|471747_472416_+\|TrkA-family-potassium-uptake-protein	gnl\|CDD\|223643	COG0569, TrkA, K+ transport systems, NAD-binding component [Inorganic ion transport and metabolism].	5.13946e-68
NZ_CP041357.1\|WP_106020318.1\|443914_445330_-\|isochorismate-synthase-MenF	gnl\|CDD\|224091	COG1169, MenF, Isochorismate synthase [Coenzyme metabolism / Secondary metabolites biosynthesis, transport, and catabolism].	9.12003e-163

>NZ_CP041357.1|WP_151174468.1|453020_454904_-|1,4-alpha-glucan-branching-enzyme
MAAASPTAHDVYLFHEGSLFKSYQLFGSHYRELNGQSGYEFCVWAPHASEVRVAGDFNSWSGELHVMQKVNDHGIWTLFVPGLKEKERYKYEIVTNSGETMLKADPYAFYSEVRPNTASLTYDLAGYSWNDQKWQKKQKAKTIYEKPVFIYELHLGSWKKHPDGTLYSYEELGQFLIPYIKEQGFTHIELLPVYEHPYDRSWGYQATGYYSPTSRFGTPHDLMRFVDECHQANIGVILDWVPGHFCKDSHGLYMFDGAPLYEYQKERDRENWLWGTANFDLGKPEVHSFLISNALYWAEFYHIDGFRVDAVANMLYWPNQDERHTNLYAVEFLKKLNQTMREAYPHVMMIAEDSTEWPQVTGSVEEGGLGFHFKWNMGWMNDVLKYMETPAEQRRHCHQLVSFSLLYAFSEHFVLPFSHDEVVYGKKSLLHKMSGDYWQKFAQYRLLLGYMTAHPGKKLIFMGSEFAQFDEWKDTEQLDWFLNSFPMHQKASVFTQDLLRLYQKSKILYENDHRAQSFEWIDVHNEEQSIFSFIRYGRRHGEALIILCNFTPSVYHQYDVGVPFLTQYIEVLNSDNEKYGGSGQLNKKPLAAKAGAVHHKPCYITMTIPPYGISIMRAVKKRGEINE
>NZ_CP041357.1|WP_151174467.1|451881_453024_-|glucose-1-phosphate-adenylyltransferase
MKKQCVAMLLAGGKGSRLSGLTKNMAKPAVSFGGKYRIIDFTLSNCSNSGIDTVGILTQYQPLELNSYIGIGSAWDLDRHNGGVTVLPPYAESSEVKWYKGTASAIYENLNYLNQYDPEYVLILSGDHIYKMDYGKMLDFHIEKKADVTISVIEVGWEEASRFGIMKTNEDGIITHFDEKPKFPKSNLASMGIYIFNWPLLKQYLEMDDRNPYSSHDFGKDIIPLLLEEKKKLSAYPFKGYWKDVGTVQSLWEANMDLLKDQSELRLFERNWKIYSVNPNQPPQFISPEAQVHDSLVNEGCVVYGNVSHSVLFQGVTVGKHATVKRSVIMPDVTIGEHVMIENAIVPKGLVLPDGAVIKSEKDIQEVLLVSEEFVEKELI
>NZ_CP041357.1|WP_095714592.1|450825_451857_-|glucose-1-phosphate-adenylyltransferase-subunit-GlgD
MINNQMLGVIDETTYKHSLQDLTAQRSLGAIPFAGRYRLIDFMLSNMVNADIRSVAIFPKYRYRSLMDHLGAGKEWDLHRKKDGLFFFPSPHLHDEYNEFGSFRQFSDHLDYFHRSTQQYAVITNSHTVCNIQFQYVLKRHQEAGCDITEVFQDGQSLQIYIMSSELLKDLIYGHIEKGYRTIQEVVEKEAPALTICPYEYSGYAAVIDSVEKYYTHSMELIQPRFWQQVFLPQQPIYTKVKDEPPTKYGKNSTVRNSLVANGCVLEGEVENCILFRAVHVGKGTKLKNCIIMQKTQIGEDCLLEQVIADKDVKIGNTTEAAGTEEQPLVLKKGLVQGELMNS
>NZ_CP041357.1|WP_151174466.1|449374_450829_-|glycogen-synthase-GlgA
MKVLFAVSECTPFVKSGGLADVAGALPKVLSRLGNDVAVIMPKYSQIPDPWKKRMKKTAECTVAVGWRQQYCGIEHLAEDGVNYYFIDNKYYFSRDSLYGHFDDGERFAYFSRAVLEAAEAVSFQADIIHTHDWHTAMVNYLLKEEYSKRPFYKQMKSVLTIHNLQFQGIFPPEVTHDLLGLDMSHFHYERLECNGFVNFMKAGILAADHVTTVSPTYRNEILTPYYGEQLEPVLQHREKDLTGILNGIDDSFYQTESDPYIEMHYDPERLDLKEENKTKLQRRMGLPERKDIPLISMVTRLTKQKGLDLVKRMMQELLGERDIQLIVLGTGEREFEDYFRYIEFAFHDKCRAYIGFDEPLAHQIYAGSDLFLMPSKFEPCGLGQMIALQYGAIPIVRETGGLYDTVRSYREEEGTGNGFTFSAFNAHDLKFTIERALSFYRQKDVWKSIMKTAMNADYSWKQSAKQYQRIFEHVTRSGRDVLE
>NZ_CP041357.1|WP_151174465.1|446991_449388_-|glycogen/starch/alpha-glucan-family-phosphorylase
MFSSKERFVALFLKRLEMTCGKSFKDSSRLDQYKTLGNMIREYISADWIQTNERSRSNSGKQTYYLSIEFLLGQLLEQNLMNLGIRDVVEAGLKEIGISLDEILYMESDAGLGNGGLGRLAACFLDSLASLNLPGHGMGIRYKHGLFEQKIVDGHQVELPEQWLKNGNVWEVRNADQAVDVPFWGEVHMTEKNGRLHFRHEQATIITAVPYDIPIIGYDTGTVNTLRLWNAEPYAHYHGGNILSYKRETEAVSEFLYPDDTHDEGKILRLKQQYFLVCASLKSIVNNYRKTNQSLYGLHKKVSIHINDTHPALAVPELMRILLDEENMSWEDAWHITVHTISYTNHTTLSEALEKWPIHLFKPLLPRMYMIIEEINERFCRAVWEKYPGDWKRIEDMAITAHGVVKMAHLAIVGSYSVNGVAKIHSDILKEREMRHFHLMFPNRFNNKTNGIAHRRWLLKANPGLSAMITDAIGDEWIKQPESLIRLEPYATDPAFIEQFQNNKSRKKQELADLIFCTAGVAVNPDSIFDVQVKRLHAYKRQLLNVLHIMYLYNRLKEDSGFSIYPQTFIFGAKASPSYHYAKKIIKLIHSVAEKVNYDPAVRQLLKVVFLENYRVSMAEQIFPASDVSEQISTASKEASGTGNMKFMMNGALTIGTHDGANIEMLQRVGADCVYTFGLKADDVLSYQENGGYRSREYYQHDRRIRQVADQLINGFFEGEADEFESIYDSLLPHNDEYFVLKDFSSYADAQERIQADYQDRRKWSKRSILNIAHSGYFSSDRTIHEYAKHIWRIKPMM
>NZ_CP041357.1|WP_151174464.1|446493_446961_+|tryptophan-rich-sensory-protein
MKKMFGAIVVFIITYALFSAADFLFPIDQSWYHSLKKPDWTPSGSTIGIIWAVLFALISLSAAIVFAKCSFKKAKSFWLAFLINYVLNQAFSYFQFTQKNLLASSIDCLLVAITALVVIIIAKVYSRAASWLLVPYFLWSAFATVLSFTIYSMNL
>NZ_CP041357.1|WP_024122606.1|445621_446341_+|yteA-family-sporulation-protein
MLTNEQLQHLKKELEQTKEDILNRFKDNDHFQLNSAFPYDSWGELSAYDNHPGDQATELYEREKDIALDLHEREHLRDIEHSLKAIENGTYGICEVSGKEIPYERLEALPTATTLAEYSSQDVVSKDRPIEEETPFGQFEFDDDEEIRAPYDSEDSYQDVEKYGNSETPQDMENPPLSYDDMTMNAEENIGNTESYENFIATDITGKEITVYPSRAHERYEEELDEEGIMTTFGDLHAD
>NZ_CP041357.1|WP_106020318.1|443914_445330_-|isochorismate-synthase-MenF
MVTTVQRTFRKEVLHALHKAKEVNHAVLISYSRQIESLDPLSFFNYGAKKYTGNRFFWSEPESELTIVGLGKEAVFQTNQKNDERYREVFEQWERFKKTAFHIYEEEKLQHSAVGPVLFGGFSFDPYEERGTQWDHFSEGDFFVPSLMLTMSADGSFLTVNRWVSGEDDAEAVLEGLKAFAAEFMVPDLNQEDQAVMTASQELDKSDWLKAIETATSQIKENQYDKVVLARELLLTFDGPVKMEPVLKTLLTDQQTSYVFAIEQEGKTFVGASPERLIKRDGNTVMSTCLAGSVKRGSDEEEDRRIGLELLNDEKNLLEHDIVVGMIHNAFVSSCSDVEKPDEPVLYKTKSVQHLFTPIVGQLHASASLFDLIEKLHPTPALGGAPQKKAVEVIREIEPMSRGWYAAPVGWIDSQDNGEFAVAIRSGLIEGSTARLFAGCGIVEDSEPQSEYEETQIKLKPMISALGGERR
>NZ_CP041357.1|WP_101863550.1|442175_443918_-|2-succinyl-5-enolpyruvyl-6-hydroxy-3--cyclohexene-1-carboxylic-acid-synthase
MTVNPITHYIGSFIDEFALSGITDAVVCPGSRSTPLAILAAAHPDINVHIQIDERSAGFFALGLAKAQQRPVLLICTSGTAAANFYPAVIEAHYSRVPIIVLTADRPHELREVGAPQAINQHFLFGNFVKFFTDSALPEESPHMLRYIRTLAGRAAGEAQKRPMGPVHVNVPLREPLMPDLSDQPFERMRTGRHVSVKTGTQSVDRDSLSDVAMILKEAEKGMIVCGELHSDADQQKIVELSKALHYPILADPLSNLRNGVHDKSTVIDAYDSFLKDDELKSALRPDVVIRFGPMPVSKPVFLWLKDDPAIQQIVIDEDGGWRDPTQASAHMIHCSPSSFADAIMAEHTGMDRTSEWMEKWQIVNQRFREYLQTVSSEEVSFEGNLYRILQHLVPENSSLFVGNSMPIRDVDTFFENQDRNFRIYSNRGANGIDGVVSSAMGVCEGTKAPVTLVIGDLSFYHDLNGLLAAKKLGIPLTVILVNNDGGGIFSFLPQASEKTHFEDLFGTPTGLDFKHAAELYGGTYSCPASWDEFKASYAPQADKPGLHIIEIKTDRQSRVQLHRDMLNEAVREVKKQWEL
>NZ_CP041357.1|WP_024122603.1|441363_442188_-|2-succinyl-6-hydroxy-2,-4-cyclohexadiene-1-carboxylate-synthase
MGTVSITVADGVRYAVTDDRPNASDAIVCLHGFTGSKQSWAFLDQLLPDSRVIKIDCLGHGESDAPLNGKRYSTSRQVSDLAEIFDHLKLHKVKLIGYSMGGRLAYSFAITYPERISALVLESTTPGLKTLGERKERILRDRKLADFILRDGIKAFVDYWEGIPLFSTQQRLAEDVRQRIRSGRLRNNNIGLANSLIGMGTGSQPSWWSRLKDIAAPVLLICGEWDEKFCAINQEVHKKLPSSRIEIVPEAGHTVHVEQPRFFGKIVSGFLTNL
>NZ_CP041357.1|WP_151174469.1|463222_463747_-|GNAT-family-N-acetyltransferase
MVTIREARLDDIKAIAKVHVDSWVTTYSGIVSAEYLNGLNYKDFESKWKSRSLKGVFVAEDEEGSVFGFASFGPIRSEQKGYDGELYAIYLLEEKQRQGAGRALLTKGTDFLLDHGFTSMFVWVVEENPSINFYHSFSPERAAEDSFEIAGESMKEIGFGWPDLASLKNLLNGR
>NZ_CP041357.1|WP_059292679.1|463773_465303_-|flotillin-lipid-rafts-scaffold-protein-FloT
MTMPIIIVIAVVFFLLIALIAVFITKYRTAGPDEALIVTGSYLGNKNVHVDEGGNRLKIVRGGGTFVLPVFQQAEPLSLLSSKLDVSTPEVYTEQGVPVMADGTAIIKIGGSIGEIATAAEQFLGKSKEDREQEAREVLEGHLRSILGSMTVEEIYKNREKFSQEVQRVASQDLAKMGLVIVSFTIKDVRDKNGYLESLGKPRIAQVKRDADIATAEADKETRIKRAEADKEAKKSELERATEIAEAEKINQLKMAEYRRDQDTAKANADQAYDLETAKARQQVTEQEMQVKIIERQKQIELEEKEILRRERQYDSEVKKKADADRYSVEQSAAAEKAKQLAQADAKKYSIEAMAKAEAERVRIDGLAKAEAEKAKGETEAEVIRLKGLAEAEAKEKIAAAFEQYGQAAIFDMIVKMLPEYAKQVSAPLSNIDKITVVDTGGNGDSSGANKVTSYATNLMSSLQESLKASSGIDVKEMIENFSGKGNIKQSINELTNELKETKPVQKSE
>NZ_CP041357.1|WP_101860056.1|465321_465846_-|hypothetical-protein
MELYGIPVQTIYLYTLIIAGSLTLLFLFFGDVFEGLSEAIPFLHPTLVLSFFTFFSASGYIGETVFPLSSLIIALISCLLSVLLVILLHLFVLVPLSSAEESLTYREDDLRGRLGKVITAVPVDGFGEVLIEGIGGTISKSAISFENEQISYGTVVLVVDVKNGVLSVTPHEPL
>NZ_CP041357.1|WP_151174470.1|466012_466501_+|DUF1569-domain-containing-protein
MSIFNEARLETWNEVKGLSDEKLNKKPSSNEWSIREVLDHLKKIDMTAQNMLKERVKEAPVKEIEEKPLTAAQDRSNKRKAPAHLEPDHDFISGSQMKRELDVVREQLTAVIASLKEEDFERVLPHPLFQELTVRQWIEFIGHHEKRHLSQIKEIKEKIERV
>NZ_CP041357.1|WP_127696596.1|466506_467085_-|MOSC-domain-containing-protein
MWKRMTAKTEGLYVADTESFITKQMDKLDFDYGGIPGDLHFGLTKKAGAREPMFSRGTEIFNRRQISIVSTEECDEIALKMGVPSIFPEWLGANVAISGIPDFTTLKEGSRIIFPSGAALLCEGENDPCIQPGEVIQSYYPDKPKLAPAFVRHALGIRGIVCIVERPGAVYAGDEIIVYSYQRNIKQKAERV
>NZ_CP041357.1|WP_024122615.1|467174_468383_-|choline-dehydrogenase
MTLNMKVESMHKFHTFEIPTVIKHGIGAINHVGKEVAGLGVSKALLVTDPGIYKAGVADPVIESLKGAGIEVVLFNKVEPNPPVRLVNEGSELYKKENCNGLVAVGGGSSMDTAKAIGVEATHEGSVLDYEAAEGKKPLENRIPPLTTIPTTAGTGSEVTQWAVITDEEREFKFNTGGPLIAAHLTVIDPELHVSMPPHVTAMTGIDALAHAIECYTMKFAQPITDAVALMAIEYAAHYIKRAFADGEDLEARYGMAQAAMLAGLSYGSESAGAAHAMSQTLGGIIPVAHGQCVAAMMGPVMEYNWKGYPEKFARIAKAFGIDTSNMTTEEAAKASVNWMYDLVKELEVPTLEEQGVSPDMIERLSKEAMKDPQTFGNPRDLNEKAYNWIYKRCFNLTPKTV
>NZ_CP041357.1|WP_151174471.1|468399_469872_-|betaine-aldehyde-dehydrogenase
MSQTLYIDGEWISAEKGQVRSIINPFNQEEIATVSEGGREDAVKAIAAARRAFDAGEWPSLSGLERGKTVLKIAELIRRDLDELAELESLDTGKTLEESKADMDDIANVFQYYAGLADKDGGEIISSPIPDSESKIIREPIGVCGQITPWNYPLLQASWKIAPALAAGNTIVMKPSEITPLTTIKVFKLMEEAGVPKGVANLVLGPGATVGDELAVNKDVDLISFTGGIETGKKIMRAASGNVKKIALELGGKNPNIVFKDADLEVAVDQALNAVFFHAGQVCSAGSRLLVEEDIHDRFLAELVKRAKRIKLGNGFHEETESGPLISAEHREKVEKYVEIGLEEGAKLETGGKRPEDPALQNGFFYEPTIFSNCKSDMRIVQEEVFGPVLTVETFSSEEEVISLANDTIYGLAGAVWSKDIEKCERVAAHLRMGTVWINDFHPYFAQAPWGGYKQSGFGRELGKIGLEEYTEVKHVYRNTKPAAVNWFNS
>NZ_CP041357.1|WP_106019451.1|470072_470615_+|GbsR/MarR-family-transcriptional-regulator
MDENPEFAAIEQARDLVIDSIAETMDLYGITRSVGILYGTMYMRDEMTLDEMREELQMSKPSMSTGVKKLQDLNVVKKTFHRGIRKHTFVAEKDFFKFFTNFFPPKWEREVQVNVSAIEEAQADLQEILHKEELNEETKNEAQRLYEQLESSKAYYDWLKRLAESVQSGEIFKFIPIETK
>NZ_CP041357.1|WP_101864219.1|470822_471368_+|biofilm-surface-layer-protein-BslA
MKRKLLSSLAISVLSLGLLVSAPSASFAAESKSSSAHTESTMRAQSTASLFATIKGASKTEWSFSDIELTYRPNTLLSLGVMEFTLPSGFTANTKDTMNGNALRETQILNNGKTVRVPLALDLLGAGEFKLKLNNKTLPAAGTYTFRAENKSLSIGNKFYAEASIEVAKRSTPPTQPCGCK
>NZ_CP041357.1|WP_024122619.1|471747_472416_+|TrkA-family-potassium-uptake-protein
MGRIKNKQFAVIGLGRFGGSICKELHRMGHEVLAIDINEEKVNAFASYATHAVIANATEENELLSLGIRNFEYVVVAIGANIQASTLTTLLLKELHIPNIWVKAQNYYHHKVLEKIGADRIIHPEKDMGVKIAQSLSDENVLNYIDLSDDYSIVELLATRKLNSKSIHDLNVRAKYGCTILAIKHNGDVRLSPAPEEVIYEQDCLVMMGHKKDIKRFENEGM

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Crispr_ID: NZ_CP041357_2

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

NZ_CP041357_2

2480112-2480241

Orphan

Consensus_repeat	Method
AAACAAACGCTCAACAAGTCAGAAAACAAAACCAACAATCAGCTGCTGG	CRISPRCasFinder

1 spacers

The CRISPR arrays of NZ_CP041357_2

>merge|NZ_CP041357|2|2480112-2480241|CRISPRCasFinder
AAACAAACGCTCAACAAGTCAGAAAACAAAACCAACAATCAGCTGCTGGTCAAGGTCAATTCGGCACTGAATTTGCTAGCGAAACAAACGCTCAGCAAGTCAGAAAACAAAACCAACAATCAGCTGCTGG

>NZ_CP041357|2|2|2480112-2480241|CRISPRCasFinder
AAACAAACGCTCAACAAGTCAGAAAACAAAACCAACAATCAGCTGCTGG	TCAAGGTCAATTCGGCACTGAATTTGCTAGCG
AAACAAACGCTCAGCAAGTCAGAAAACAAAACCAACAATCAGCTGCTGG

Protein	Signature genes	Signature genes Name	Protein_function
NZ_CP041357.1\|WP_024120665.1\|2486072_2486546_+\|thioredoxin-dependent-thiol-peroxidase	unknown	unknown	gnl\|CDD\|224146
NZ_CP041357.1\|WP_010333472.1\|2486680_2487118_+\|peroxide-responsive-transcriptional-repressor-PerR	unknown	unknown	gnl\|CDD\|223806
NZ_CP041357.1\|WP_044163733.1\|2470185_2470569_-\|hypothetical-protein	unknown	unknown	unknown
NZ_CP041357.1\|WP_024120657.1\|2476634_2477618_-\|metal-dependent-hydrolase	unknown	unknown	gnl\|CDD\|224899
NZ_CP041357.1\|WP_151174857.1\|2470975_2471494_+\|SH3-domain-containing-protein	unknown	unknown	gnl\|CDD\|377965
NZ_CP041357.1\|WP_151174860.1\|2487707_2488592_+\|hypothetical-protein	unknown	unknown	gnl\|CDD\|379641
NZ_CP041357.1\|WP_106021581.1\|2484631_2485921_-\|glutamate-1-semialdehyde-2,1-aminomutase	unknown	unknown	gnl\|CDD\|183493
NZ_CP041357.1\|WP_127695642.1\|2481536_2483279_+\|ABC-transporter-ATP-binding-protein	unknown	unknown	gnl\|CDD\|224055
NZ_CP041357.1\|WP_003239685.1\|2480921_2481452_+\|DUF402-domain-containing-protein	unknown	unknown	gnl\|CDD\|184219
NZ_CP041357.1\|WP_151174858.1\|2474052_2476638_+\|YfhO-family-protein	unknown	unknown	gnl\|CDD\|226888
NZ_CP041357.1\|WP_106022511.1\|2495555_2496332_-\|sporulation-protein	unknown	unknown	gnl\|CDD\|226776
NZ_CP041357.1\|WP_024120663.1\|2483351_2484413_-\|aromatic-acid-exporter-family-protein	unknown	unknown	gnl\|CDD\|226614
NZ_CP041357.1\|WP_010333455.1\|2470570_2470783_-\|helix-turn-helix-transcriptional-regulator	unknown	unknown	gnl\|CDD\|224393
NZ_CP041357.1\|WP_127695640.1\|2487148_2487502_-\|YgzB-family-protein	unknown	unknown	gnl\|CDD\|371346
NZ_CP041357.1\|WP_127695650.1\|2471579_2471912_+\|hypothetical-protein	unknown	unknown	gnl\|CDD\|227945
NZ_CP041357.1\|WP_044163745.1\|2479263_2480016_+\|enoyl-[acyl-carrier-protein]-reductase-FabL	unknown	unknown	gnl\|CDD\|236145
NZ_CP041357.1\|WP_024120659.1\|2478956_2479181_-\|hypothetical-protein	unknown	unknown	unknown
NZ_CP041357.1\|WP_010333459.1\|2472993_2473983_+\|glycosyltransferase-family-2-protein	unknown	unknown	gnl\|CDD\|133030
NZ_CP041357.1\|WP_101860263.1\|2471898_2472762_+\|alpha/beta-hydrolase	unknown	unknown	gnl\|CDD\|223669
NZ_CP041357.1\|WP_151174859.1\|2480517_2480778_+\|hypothetical-protein	unknown	unknown	gnl\|CDD\|372951

Protein	Function_ID	Function_description	E-value
NZ_CP041357.1\|WP_024120665.1\|2486072_2486546_+\|thioredoxin-dependent-thiol-peroxidase	gnl\|CDD\|224146	COG1225, Bcp, Peroxiredoxin [Posttranslational modification, protein turnover, chaperones].	3.77251e-85
NZ_CP041357.1\|WP_010333472.1\|2486680_2487118_+\|peroxide-responsive-transcriptional-repressor-PerR	gnl\|CDD\|223806	COG0735, Fur, Fe2+/Zn2+ uptake regulation proteins [Inorganic ion transport and metabolism].	1.43425e-56
NZ_CP041357.1\|WP_127695640.1\|2487148_2487502_-\|YgzB-family-protein	gnl\|CDD\|371346	pfam11023, DUF2614, Zinc-ribbon containing domain. This is a family of proteins conserved in the Bacillaceae family. Some members are annotated as being protein YgzB. The function is not known.	7.93089e-70
NZ_CP041357.1\|WP_151174857.1\|2470975_2471494_+\|SH3-domain-containing-protein	gnl\|CDD\|377965	pfam08239, SH3_3, Bacterial SH3 domain.	1.48485e-10
NZ_CP041357.1\|WP_151174860.1\|2487707_2488592_+\|hypothetical-protein	gnl\|CDD\|379641	pfam14540, NTF-like, Nucleotidyltransferase-like. Structural comparisons with Structure 1kny indicate that this N-terminal domain resembles a nucleotidyltransferase fold.	5.98351e-57
NZ_CP041357.1\|WP_106021581.1\|2484631_2485921_-\|glutamate-1-semialdehyde-2,1-aminomutase	gnl\|CDD\|183493	PRK12389, PRK12389, glutamate-1-semialdehyde aminotransferase; Provisional.	0
NZ_CP041357.1\|WP_127695642.1\|2481536_2483279_+\|ABC-transporter-ATP-binding-protein	gnl\|CDD\|224055	COG1132, MdlB, ABC-type multidrug transport system, ATPase and permease components [Defense mechanisms].	0
NZ_CP041357.1\|WP_003239685.1\|2480921_2481452_+\|DUF402-domain-containing-protein	gnl\|CDD\|184219	PRK13662, PRK13662, hypothetical protein; Provisional.	1.54037e-126
NZ_CP041357.1\|WP_151174858.1\|2474052_2476638_+\|YfhO-family-protein	gnl\|CDD\|226888	COG4485, COG4485, Predicted membrane protein [Function unknown].	0
NZ_CP041357.1\|WP_106022511.1\|2495555_2496332_-\|sporulation-protein	gnl\|CDD\|226776	COG4326, Spo0M, Sporulation control protein [General function prediction only].	1.11279e-147
NZ_CP041357.1\|WP_024120663.1\|2483351_2484413_-\|aromatic-acid-exporter-family-protein	gnl\|CDD\|226614	COG4129, COG4129, Predicted membrane protein [Function unknown].	6.30372e-88
NZ_CP041357.1\|WP_010333455.1\|2470570_2470783_-\|helix-turn-helix-transcriptional-regulator	gnl\|CDD\|224393	COG1476, COG1476, Predicted transcriptional regulators [Transcription].	8.44969e-20
NZ_CP041357.1\|WP_010333459.1\|2472993_2473983_+\|glycosyltransferase-family-2-protein	gnl\|CDD\|133030	cd04187, DPM1_like_bac, Bacterial DPM1_like enzymes are related to eukaryotic DPM1. A family of bacterial enzymes related to eukaryotic DPM1; Although the mechanism of eukaryotic enzyme is well studied, the mechanism of the bacterial enzymes is not well understood. The eukaryotic DPM1 is the catalytic subunit of eukaryotic Dolichol-phosphate mannose (DPM) synthase. DPM synthase is required for synthesis of the glycosylphosphatidylinositol (GPI) anchor, N-glycan precursor, protein O-mannose, and C-mannose. The enzyme has three subunits, DPM1, DPM2 and DPM3. DPM is synthesized from dolichol phosphate and GDP-Man on the cytosolic surface of the ER membrane by DPM synthase and then is flipped onto the luminal side and used as a donor substrate. This protein family belongs to Glycosyltransferase 2 superfamily.	2.17286e-84
NZ_CP041357.1\|WP_044163745.1\|2479263_2480016_+\|enoyl-[acyl-carrier-protein]-reductase-FabL	gnl\|CDD\|236145	PRK08063, PRK08063, enoyl-[acyl-carrier-protein] reductase FabL.	6.20601e-172
NZ_CP041357.1\|WP_127695650.1\|2471579_2471912_+\|hypothetical-protein	gnl\|CDD\|227945	COG5658, COG5658, Predicted integral membrane protein [Function unknown].	1.0817e-15
NZ_CP041357.1\|WP_024120657.1\|2476634_2477618_-\|metal-dependent-hydrolase	gnl\|CDD\|224899	COG1988, COG1988, Predicted membrane-bound metal-dependent hydrolases [General function prediction only].	7.26399e-44
NZ_CP041357.1\|WP_101860263.1\|2471898_2472762_+\|alpha/beta-hydrolase	gnl\|CDD\|223669	COG0596, MhpC, Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily) [General function prediction only].	1.08581e-38
NZ_CP041357.1\|WP_151174859.1\|2480517_2480778_+\|hypothetical-protein	gnl\|CDD\|372951	pfam14182, YgaB, YgaB-like protein. The YgaB-like protein family includes the B. subtilis YgaB protein, which is functionally uncharacterized. This family of proteins is found in bacteria. Proteins in this family are approximately 90 amino acids in length.	2.99792e-26

>NZ_CP041357.1|WP_044163745.1|2479263_2480016_+|enoyl-[acyl-carrier-protein]-reductase-FabL
MEQNKCALVTGSSRGVGKAAAIRLAENGYNIVINYARSKKAALETAEEIEKLGVKVLTVKANVGQPAKIKEMFQQIDETFGRLDVFVNNAASGVLRPVMELEETHWDWTMNINAKALLFCAQEAAKLMEKNGGGHIVSISSLGSIRYLENYTTVGVSKAALEALTRYLAVELSPKQIIVNAVSGGAIDTDALKHFPNREDLLEDARQNTPAGRMVEIKDMVDTVEFLVSSKADMIRGQTIIVDGGRSLLV
>NZ_CP041357.1|WP_024120659.1|2478956_2479181_-|hypothetical-protein
MYVGRDMSELTMISKKDWKNSELAFFHHAFQQIMPYLNEEGQSKYRELTQEIEARGGMKRNEADYSHGTRVSYD
>NZ_CP041357.1|WP_024120657.1|2476634_2477618_-|metal-dependent-hydrolase
MDTGTHVVMGIALGGIATLDPVVGTDPAMAHAVMIATLAGSQAPDIDTVLKLKNNAVYIRNHRGFTHSIPAVLFWSVIIPAILYLFYPQADFLHLWLWTLLAVVLHVFVDIFNAYGTQAIRPFSKKWVALGLINTFDPFIFISHLAAIAVWYVGGSPGITFLTLYVILAGYYILRLMMQLRIKRQLREMIHEEIESIIISPTMKFRQWRIAVTTAHAFYVGRSMDGHVVILDTFNRVPVPETDVMQAAKQDDNIAAFLSFSPVYRWEVDTYKDHYEVRFIDLRYRSKGHYPFVAIVHISHDLEIRSSYTGWIFSEEKLQKKLKLGSI
>NZ_CP041357.1|WP_151174858.1|2474052_2476638_+|YfhO-family-protein
MKKRKHVMIYAASLFIAVLAHAFFVKEWAEGRYMTGPGDGLAQMIVFKKLLYEQYTQGNFFYHYSFGLGGGTFSQLGYYFSASFLFLATSAAVWLLQSVHLIGEADTLFWAQSAVFISIIKLSLIIFTAASVFSYLLKRRAAAFTGAVLYGVSIIYFRHEAYWEFFTDTMIWLPLLVLGAEKIIRERRPAWFIIACSLSLINNFYFAYINLIFIGIYILFRWLIKLEDHEVKKWVQCRMFLVSGLISFGISAAVFVPVVYGYLNNLRPPYNEKIEWLNFDDNILFSSRIIVVPAIFLLFLFVISFYKHRVFRLFAGLSLLFILFHFSPYAASAFNGFSAPQNRFEYILAFTIAGAAAAGLSQLQELRRKELLPAAAIVLLLYWYHVQRYELDIWKPANESILLLLFLTIAALFTAVFVKKRAKLAVYGVVILSSLLVANTYQKYALSEGGGLDSVSKSYITSEEYKGDEQSQLIRRVQKEDNDPLVRIDWMNGVRNNTPIIYGFNGFSAYSSILNSHLLSFYWNDLRIDMGRESVSRYASLGDRANLYSLLYGRYYMTEKINEANVPYGFTKHIESEHYTVYENQYMLPFVKSADKVYSERDLEKLPALAKEQAMLKGIVLADPTEKTEQPPDLANLISKSDITAKQAQYQNGLLMVTGENGGELIITPKKASAGPGDYYVSFYLKSKAKDKGFTLKVNDYVTTRKSNQSIYKTGVNDLTIRVPKSDHISIRLPKGTYELSHIALYAENYQTLKNAVSQNKTERTDSLEWNKNKLTFTYHNSKPQYIMLPIPYEKGWELKINGKQQKIEKADYTFIGFKAQKGDNQIELTYYPPYFKISAVMSFISLLLAVFYTRRKKARL
>NZ_CP041357.1|WP_010333459.1|2472993_2473983_+|glycosyltransferase-family-2-protein
MKQGLISIIIPSYNEGYNVKLIHESLKKEFKNIHYDYEIFFINDGSIDDTLQQIKDLASKCSRVKYISFSRNFGKEAAILAGFEHVQGEAVVVMDADLQHPTYLLKDFIKGYEEGYDQVIAQRNRKGDNPVRSLLSSLYYKFINKAVEVDLRDGVGDFRLLSRQAVDALLKLSEGNRFSKGLFCWIGFEQKIVFYENVERKNGTSKWSFSSLFNYGMDGVVSFNHKPLRICFYTGIFVLLLSLIYIIATFVKILTNGISVPGYFTIISAVLFLGGVQLLSLGIIGEYIGRIYYETKKRPHYLIKEANIPSKDLPETNELKNMRRLTKLH
>NZ_CP041357.1|WP_101860263.1|2471898_2472762_+|alpha/beta-hydrolase
MEGVNSQFVKTNGITLHVASAGPEDGPLIVLLHGFPEFWYGWKNQIKPLADAGYRVIAPDQRGYNLSDKPEGIEAYRIDTLRDDIIGLISQFTDQKAIVIGHDWGGAVAWHLASTRPEYLEKLIAINIPHPHVMKTVTPVYPPQWLKSSYIAFFQLPDKPEAALTENDYEKLDKAIGLSDRPELFSSEDINKYKEAWKQPGALTSMLNWYRALRKGSLAENSRECHTAPYRMIWGMEDRFLSRKLAKETARRCPNGHLIFVDEASHWISHEKPAIVNQLILEYLENQ
>NZ_CP041357.1|WP_127695650.1|2471579_2471912_+|hypothetical-protein
MACLVGGGLMMIAGLFIKLFPPKSINSIYGFRTRRSMSDQKLWNEANRYSAALMILSGLIVLGAGVLLRSSFIILQLILLVAACIITFILTEKRLKHMTQSQGGDLSGRS
>NZ_CP041357.1|WP_151174857.1|2470975_2471494_+|SH3-domain-containing-protein
MKKKQVLLALTAAAGLSFTACSTAPAAKAATLQTINVSMPSSDSYMIKAGKLNVRAEPKQKSAILGTVSSEQKVTVKGFANADWAQIEFKGKKGYISTHFLMKTASQAKTAKQTAFYSPTPENGKAKQLSSGTEVTLLGWGFSENGGFDFSWAFVDHGGIKGYIPTKDLQIR
>NZ_CP041357.1|WP_010333455.1|2470570_2470783_-|helix-turn-helix-transcriptional-regulator
MKHGVRNCVKEYRKAKGWTQDQLAENIQVTRQTVIAIEKQRYEPSIGTAIKLSSVLECSLDQLFWIEGDK
>NZ_CP041357.1|WP_044163733.1|2470185_2470569_-|hypothetical-protein
MNMTYRTGLFLQTLLPILLYVLYWHSPSTLTLLLAVIGTARVLYSAAHIHRNKLWGNVHFQNDQRTRSNAHFAGYISHWVMIVILLIGVVLIHQSMLPLSYLQLIAYTAFLGFMLRMVMSDVLNTNV
>NZ_CP041357.1|WP_151174859.1|2480517_2480778_+|hypothetical-protein
MTEFEKLVIEQMKTMDKLLDLQSELDRCKEIEAKLRHLERDARLRGIQDEIAVKRKHLADIQDTFQKQTEQVIRSYRSSEKPSSYV
>NZ_CP041357.1|WP_003239685.1|2480921_2481452_+|DUF402-domain-containing-protein
MGYPKEGETIQIHSYKHNGLIHRIWNETTILKSTEMCVIGANDRTMVTESDGRTWITREPAICYFHARQWFNVIGMLREDGVHYYCNISSPFAYDGEAIKYIDYDLDVKVFPDMTYNILDEDEYDDHRKSMNYPKEIDSILRDYLNTLLHWIHQRQGPFAPEFVDMWYERYLRYTK
>NZ_CP041357.1|WP_127695642.1|2481536_2483279_+|ABC-transporter-ATP-binding-protein
MGVMKRYMQFVKPYKKQIFITVLIGIVKFSIPLALPLLLKYVVDDIIQGGGTASDKTTSLFTIMAIMFALFLILRPPVEYYRQYFAQWTASKVLYDIRAKLFDHIQKLSLRFYANTRTGEVISRVINDVEQTKDFVITGLMNIWLDMLTILIVISIMLTLDVKLTLISIILFPLYGISVKYFYGRLRKLTRERSQALAQVQGHLHERIQGMPVIRSFAIEDHEQANFNEKNSHFLDKAIRHTNWNAKTFAVVNTITDLAPLIVIAFAGYFVINGPLTVGTMVAFVGYIDRMYNPVRRLINSSTTLTQSIASMDRVFEFIDEPYELTDKPNAIKADHIRGEVEFRNVSFQYEKGKENILHDVSLKVTRGETVALVGMSGGGKSTLVSLIPRFYDVSSGKLLIDGTDIRDYEARSLRNQVGMVLQDTFLFSETIRENIAIGKPDATLEDIMEAAKAANAHDFIMSFPEGYETRVGERGVKLSGGQKQRISIARVFLKNPPLLILDEATSALDLESEHYIQEAMDKLAKDRTTFVVAHRLSTITHADKIVVMENGTIIETGTHDELMDYDSQYKHLFTIQNLN
>NZ_CP041357.1|WP_024120663.1|2483351_2484413_-|aromatic-acid-exporter-family-protein
MKLGARIFKTGIAITLALYLASWIGLPAPIFAGIAAIFAIQPSIYRSFLIIIDQVQANIIGAVIATVFGLIFGPSPIMIGLTAVIVITIMLKLKIEHTISIALVTVIAILESAGDDFLMFALIRTSTVILGVLSSFIVNLVFLPPKYETKLIHNTVENTEEIMKWIRLIMRQSTEHSILKEDIEKLKEKMIKLDQTYLLYKEERSYFKKTTYVKSRKLVLFRQAIITANRALDTLKKLHRLENEIYHMPEEFQETLTEELDYLLYWHERILMRFVGKIKPHDDTDEEGIQYKQLLTKSFLKNQRNSDDELLDYNMLNIMASAVEYREQLEHLETLITSFQTYHPKDCEMEIEE
>NZ_CP041357.1|WP_106021581.1|2484631_2485921_-|glutamate-1-semialdehyde-2,1-aminomutase
MYTKSVELHREALEHIVGGVNSPSRSYKAVGGGSPVAMEKANGAYFWDVDGNKYIDYLAAYGPIITGHAHPHITEAIKKAAENGVLYGTPTKHEVTFAKMLKEAIPAMDKVRFVNSGTEAVMTTIRVARAYTGRTKIIKFAGCYHGHSDLVLVAAGSGPSTLGTPDSAGVPKSIANEVITVPFNDIDSYKAALEKWGSEIAAVLVEPIVGNFGIVEPKEGFLEQVNELTHNAGALVIYDEVITAFRFMYGGAQDLLQVKPDLTALGKIIGGGLPIGAYGGKQEIMEQVAPLGPAYQAGTMAGNPASILSGIACLEVLKEEGVYEKLDHLGAMLEEGILAHAETYGITITVNRLKGALTVYFSDEKVENYDQAERSDGETFATFFKLMLERGINLAPSKYEAWFITTAHTEQDIKDTLAAVEDAFKHLKK
>NZ_CP041357.1|WP_024120665.1|2486072_2486546_+|thioredoxin-dependent-thiol-peroxidase
MTIEIGQKAPDIELKGDHGEIVKLSDYEGKYIVLYFYPKDMTPGCTTEACDFRDSHENFAELDAVIIGVSPDSEEKHGKFKEKHNLPFLLLVDDEHKLAEAFDVWKLKKNFGKEYMGIERSTFLIDKEGRLIKEWRKVKVKDHVAEALQTLKDMSGK
>NZ_CP041357.1|WP_010333472.1|2486680_2487118_+|peroxide-responsive-transcriptional-repressor-PerR
MAAHELKEALETLKETGVRITPQRHAILEYLVNSMAHPTADDIYKALEGKFPNMSVATVYNNLRVFRESGLVKELTYGDASSRFDFVTSDHYHAICESCGKIVDFHYPGLDEVEQLAAHVTGFKVSHHRLEIYGVCQECSKKENH
>NZ_CP041357.1|WP_127695640.1|2487148_2487502_-|YgzB-family-protein
MAKYSSKINKIRTFALSLVFVGFIIMYIGIFFKESVLLSSLFMILGLLSIGLSTVVYFWIGMLSTKAVRVICPGCKKETKVLGRVDMCMHCREPLTLDQSLEGKEFDESYNKKKMSK
>NZ_CP041357.1|WP_151174860.1|2487707_2488592_+|hypothetical-protein
MENLLRPIYQERASHPNTLAVIMIERRNKTSSITDNFDAALLVIVKQSDKPVFIKHYEFDHKTASLHVITDAQVQEWILLGTNRRIIDWIVNGRVMFDRNEYIAELIDRLNTFPFAERKLKIGFEYGKLIRRYVEGKAFFEANQFLDAYNAVVHALHHLARIEVIDRGFHPETTVWSQVRQMEPQVYKLYSELIESHESLEKRLELLFLANDFLIHSKAEIGSAHLFEVMKEKDIWQFSELLQHPDLKHFTQDLGVMLDYLTEKGLISVCRIETKGQAVFHRGYYFKKDVDSDS
>NZ_CP041357.1|WP_106022511.1|2495555_2496332_-|sporulation-protein
MSFFKKLAASAGIGAAKVDTILEKDAYCPGEEVRGTVHVKGGKIAQDIRYIDLQLNTRYVIVKDDEERRKHATIHSFRVTGSFTIQPGEEHQFPFSFILPLDTPITVGKVEVAVVTDLDIQGGIDKSDHDRIFVEAHPWVENVLEAIENLGFRLNEADCEQASYFQRRLPFVQEFEFVPTSGYYRQMLDELELIFLLEEDGLEIIFEVDRRARGLHGWLEEMYNDGEQLVRVRFRQSELDNIEQLEEVLEEIIDQYAE

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Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity
NZ_CP041357_1	1.1\|455529\|57\|NZ_CP041357\|CRISPRCasFinder	455529-455585	57	NC_020451	Vibrio coralliilyticus strain ATCC BAA-450 plasmid, complete sequence	1963-2019	7	0.877
NZ_CP041357_1	1.1\|455529\|57\|NZ_CP041357\|CRISPRCasFinder	455529-455585	57	NZ_CP015439	Anoxybacillus amylolyticus strain DSM 15939 plasmid pDSM15939_1, complete sequence	255887-255943	7	0.877
NZ_CP041357_1	1.1\|455529\|57\|NZ_CP041357\|CRISPRCasFinder	455529-455585	57	NZ_CP024037	Bacillus aryabhattai strain K13 plasmid unnamed2	10420-10476	8	0.86
NZ_CP041357_1	1.1\|455529\|57\|NZ_CP041357\|CRISPRCasFinder	455529-455585	57	NZ_CP026740	Bacillus megaterium strain YC4-R4 plasmid unnamed4	70767-70823	8	0.86
NZ_CP041357_1	1.1\|455529\|57\|NZ_CP041357\|CRISPRCasFinder	455529-455585	57	NZ_CP010587	Bacillus megaterium Q3 plasmid p1, complete sequence	838-894	8	0.86
NZ_CP041357_1	1.1\|455529\|57\|NZ_CP041357\|CRISPRCasFinder	455529-455585	57	NZ_CP023319	Bacillus megaterium strain A plasmid p2, complete sequence	75825-75881	8	0.86
NZ_CP041357_1	1.1\|455529\|57\|NZ_CP041357\|CRISPRCasFinder	455529-455585	57	NC_017139	Bacillus megaterium WSH-002 plasmid WSH-002_p1, complete sequence	1321-1377	8	0.86
NZ_CP041357_2	2.1\|2480161\|32\|NZ_CP041357\|CRISPRCasFinder	2480161-2480192	32	NZ_CP037418	Bacillus velezensis strain LB002 plasmid unnamed1	24866-24897	8	0.75
NZ_CP041357_1	1.1\|455529\|57\|NZ_CP041357\|CRISPRCasFinder	455529-455585	57	NZ_CP033045	Bacillus foraminis strain Bac44 plasmid unnamed, complete sequence	483791-483847	9	0.842
NZ_CP041357_1	1.1\|455529\|57\|NZ_CP041357\|CRISPRCasFinder	455529-455585	57	NZ_CP015440	Anoxybacillus amylolyticus strain DSM 15939 plasmid pDSM15939_2, complete sequence	64776-64832	10	0.825
NZ_CP041357_1	1.1\|455529\|57\|NZ_CP041357\|CRISPRCasFinder	455529-455585	57	NZ_LR134399	Listeria monocytogenes strain NCTC7974 plasmid 2, complete sequence	57361-57417	12	0.789
NZ_CP041357_1	1.1\|455529\|57\|NZ_CP041357\|CRISPRCasFinder	455529-455585	57	NC_048753	Lactobacillus phage 3-521, complete genome	13555-13611	13	0.772

1. spacer 1.1|455529|57|NZ_CP041357|CRISPRCasFinder matches to NC_020451 (Vibrio coralliilyticus strain ATCC BAA-450 plasmid, complete sequence) position: , mismatch: 7, identity: 0.877

cagcttggaaggctgaggttttaccactaaactacacccgcaatt---ttttggggcgat	CRISPR spacer
cagcttggaaggctgaggttttaccactaaactacacccgcattttagttatggccc---	Protospacer
****************************************** **   ** ***  *

2. spacer 1.1|455529|57|NZ_CP041357|CRISPRCasFinder matches to NZ_CP015439 (Anoxybacillus amylolyticus strain DSM 15939 plasmid pDSM15939_1, complete sequence) position: , mismatch: 7, identity: 0.877

cagcttggaaggctgaggttttaccactaaactacacccgca-attttttggggcgat	CRISPR spacer
cagcttggaaggctgaggttttaccactaaactacacccgcatattatatacgacgg-	Protospacer
****************************************** *** * *. *.**.

3. spacer 1.1|455529|57|NZ_CP041357|CRISPRCasFinder matches to NZ_CP024037 (Bacillus aryabhattai strain K13 plasmid unnamed2) position: , mismatch: 8, identity: 0.86

cagcttggaaggctgaggttttaccactaaactacacccgcaattttttggggcgat-	CRISPR spacer
cagcttggaaggctgaggttttaccactaaactacacccgc-atattttaaagtggcg	Protospacer
***************************************** ** ****...*.*..

4. spacer 1.1|455529|57|NZ_CP041357|CRISPRCasFinder matches to NZ_CP026740 (Bacillus megaterium strain YC4-R4 plasmid unnamed4) position: , mismatch: 8, identity: 0.86

cagcttggaaggctgaggttttaccactaaactacacccgcaattttttggggcgat-	CRISPR spacer
cagcttggaaggctgaggttttaccactaaactacacccgc-atattttaaagtggcg	Protospacer
***************************************** ** ****...*.*..

5. spacer 1.1|455529|57|NZ_CP041357|CRISPRCasFinder matches to NZ_CP010587 (Bacillus megaterium Q3 plasmid p1, complete sequence) position: , mismatch: 8, identity: 0.86

cagcttggaaggctgaggttttaccactaaactacacccgcaattttttggggcgat-	CRISPR spacer
cagcttggaaggctgaggttttaccactaaactacacccgc-atattttaaagtggcg	Protospacer
***************************************** ** ****...*.*..

6. spacer 1.1|455529|57|NZ_CP041357|CRISPRCasFinder matches to NZ_CP023319 (Bacillus megaterium strain A plasmid p2, complete sequence) position: , mismatch: 8, identity: 0.86

cagcttggaaggctgaggttttaccactaaactacacccgcaattttttggggcgat-	CRISPR spacer
cagcttggaaggctgaggttttaccactaaactacacccgc-atattttaaagtggcg	Protospacer
***************************************** ** ****...*.*..

7. spacer 1.1|455529|57|NZ_CP041357|CRISPRCasFinder matches to NC_017139 (Bacillus megaterium WSH-002 plasmid WSH-002_p1, complete sequence) position: , mismatch: 8, identity: 0.86

cagcttggaaggctgaggttttaccactaaactacacccgcaattttttggggcgat-	CRISPR spacer
cagcttggaaggctgaggttttaccactaaactacacccgc-atattttaaagtggcg	Protospacer
***************************************** ** ****...*.*..

8. spacer 2.1|2480161|32|NZ_CP041357|CRISPRCasFinder matches to NZ_CP037418 (Bacillus velezensis strain LB002 plasmid unnamed1) position: , mismatch: 8, identity: 0.75

tcaaggtcaattcggcactgaatttgctagcg--	CRISPR spacer
tcagggtcaatacggcactgaatc--gcagtgaa	Protospacer
***.******* ***********.   .**.*

9. spacer 1.1|455529|57|NZ_CP041357|CRISPRCasFinder matches to NZ_CP033045 (Bacillus foraminis strain Bac44 plasmid unnamed, complete sequence) position: , mismatch: 9, identity: 0.842

cagcttggaaggctgaggttttaccactaaactacacccgc-------aattttttgggg	CRISPR spacer
cagcttggaaggctgaggttttaccactaaactacacccgcatggttaaaagttttg---	Protospacer
*****************************************       **  *****

10. spacer 1.1|455529|57|NZ_CP041357|CRISPRCasFinder matches to NZ_CP015440 (Anoxybacillus amylolyticus strain DSM 15939 plasmid pDSM15939_2, complete sequence) position: , mismatch: 10, identity: 0.825

cagcttggaaggctgaggttttaccactaaactacacccgc--aattttttggggcgat	CRISPR spacer
cagcttggaaggctgaggttttaccactaaactacacccgcataataatatatacca--	Protospacer
*****************************************  ***  * *. . *.

11. spacer 1.1|455529|57|NZ_CP041357|CRISPRCasFinder matches to NZ_LR134399 (Listeria monocytogenes strain NCTC7974 plasmid 2, complete sequence) position: , mismatch: 12, identity: 0.789

cagcttggaaggctgaggttttaccactaaactacacccgca--------attttttggg	CRISPR spacer
cagcttggaaggctgtagttttaccactaaactacacccgcatagtaagtagttctt---	Protospacer
*************** .*************************        * **.**

12. spacer 1.1|455529|57|NZ_CP041357|CRISPRCasFinder matches to NC_048753 (Lactobacillus phage 3-521, complete genome) position: , mismatch: 13, identity: 0.772

cagcttggaaggctgaggttttaccactaaactacacccgc---aattttttggggcgat	CRISPR spacer
cagcttggaaggctgtggttttaccactaaactatacccgcgtgaactactcgtcat---	Protospacer
*************** ******************.******   **.* .*.*  ..

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

722380 : 755154

Staphylococcus_phage(20.0%)

holin,transposase

The bacterium proteins that are colored denote the protein is present at specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin' and 'tRNA')

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_151174509.1\|722380_723530_+\|transposase	IS3 family transposase	A0A0N9SIX5	Staphylococcus_phage	1.0e-38	60.4
WP_059292555.1\|723589_724006_-	disulfide bond formation protein B	NA	NA	NA	NA
WP_101859952.1\|724010_724679_-	DsbA family protein	NA	NA	NA	NA
WP_106019556.1\|724797_726909_-	cadmium-translocating P-type ATPase	E4ZFI9	Streptococcus_phage	9.7e-120	36.6
WP_127696475.1\|727077_729489_-	copper-translocating P-type ATPase	A0A218MNH6	uncultured_virus	1.5e-121	38.1
WP_024122844.1\|729571_729781_-	copper chaperone CopZ	NA	NA	NA	NA
WP_024122845.1\|729856_730159_-	copper-sensing transcriptional repressor CsoR	NA	NA	NA	NA
WP_127696474.1\|730316_731363_+	oxidoreductase	NA	NA	NA	NA
WP_101864801.1\|731397_732024_-	FMN-dependent NADH-azoreductase	NA	NA	NA	NA
WP_151174510.1\|732185_734084_-	FUSC family protein	NA	NA	NA	NA
WP_151174511.1\|734219_734876_-	FCD domain-containing protein	NA	NA	NA	NA
WP_095713019.1\|734882_735830_-	EamA family transporter	NA	NA	NA	NA
WP_095713018.1\|735924_736314_-	YvaD family protein	NA	NA	NA	NA
WP_106019560.1\|736315_736669_-	multidrug efflux SMR transporter	NA	NA	NA	NA
WP_044159119.1\|736684_737239_-	TetR/AcrR family transcriptional regulator	NA	NA	NA	NA
WP_106019561.1\|737352_738147_-	SDR family oxidoreductase	NA	NA	NA	NA
WP_151174512.1\|738864_739335_-	SsrA-binding protein	W5RAM5	Staphylococcus_phage	7.0e-47	62.8
WP_151174513.1\|739478_741815_-	ribonuclease R	Q0GXV6	Lactococcus_phage	5.5e-92	41.1
WP_044159124.1\|741833_742574_-	alpha/beta fold hydrolase	NA	NA	NA	NA
WP_003220028.1\|742705_742936_-	preprotein translocase subunit SecG	NA	NA	NA	NA
WP_151174514.1\|743089_743854_+	alpha/beta fold hydrolase	NA	NA	NA	NA
WP_024122859.1\|743897_744131_-	helix-turn-helix transcriptional regulator	NA	NA	NA	NA
WP_106019564.1\|744282_744690_+	transcriptional repressor RghR	S6C481	Thermus_phage	1.5e-16	64.8
WP_024122861.1\|744718_745138_+	helix-turn-helix transcriptional regulator	S6C481	Thermus_phage	7.0e-14	62.7
WP_010331897.1\|745229_745556_+	catDE operon transcriptional regulator CatR	NA	NA	NA	NA
WP_024122862.1\|745682_747383_+	methyl-accepting chemotaxis protein	A0A2H4J162	uncultured_Caudovirales_phage	1.4e-20	34.3
WP_024122863.1\|747428_748109_-\|holin	choline ABC transporter permease OpuBD	NA	NA	NA	NA
WP_101864780.1\|748124_749045_-	osmoprotectant ABC transporter substrate-binding protein	NA	NA	NA	NA
WP_095713009.1\|749056_749710_-	ABC transporter permease	NA	NA	NA	NA
WP_101864778.1\|749724_750870_-\|holin	betaine/proline/choline family ABC transporter ATP-binding protein	F2Y1V5	Organic_Lake_phycodnavirus	9.5e-13	30.0
WP_106019566.1\|751154_751688_+	GbsR/MarR family transcriptional regulator	NA	NA	NA	NA
WP_101864776.1\|751722_752391_-\|holin	glycine betaine/carnitine/choline/choline sulfate ABC transporter permease OpuCD	NA	NA	NA	NA
WP_106019934.1\|752408_753275_-	osmoprotectant ABC transporter substrate-binding protein	NA	NA	NA	NA
WP_024122875.1\|753339_753993_-\|holin	glycine betaine/carnitine/choline/choline sulfate ABC transporter permease OpuCB	NA	NA	NA	NA
WP_101859940.1\|754014_755154_-\|holin	betaine/proline/choline family ABC transporter ATP-binding protein	W6JKT0	Anomala_cuprea_entomopoxvirus	3.6e-12	29.8

DBSCAN-SWA_2

1129343 : 1182867

Bacillus_phage(20.0%)

protease,tRNA,bacteriocin,coat

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_106019730.1\|1129343_1131014_-\|tRNA	arginine--tRNA ligase	NA	NA	NA	NA
WP_024123211.1\|1131010_1131439_-	DUF1934 domain-containing protein	NA	NA	NA	NA
WP_003222002.1\|1131750_1131882_+\|bacteriocin	subtilosin A family bacteriocin	NA	NA	NA	NA
WP_044159802.1\|1132016_1133363_+	subtilosin maturase AlbA	NA	NA	NA	NA
WP_059292334.1\|1133375_1133537_+	hypothetical protein	NA	NA	NA	NA
WP_024123214.1\|1133533_1134253_+	ABC transporter ATP-binding protein	A0A2H4PQG7	Staphylococcus_phage	1.2e-18	27.6
WP_106019944.1\|1135546_1136710_+	insulinase family protein	NA	NA	NA	NA
WP_151174591.1\|1137988_1138690_+\|bacteriocin	bacteriocin biosynthesis protein AlbG	NA	NA	NA	NA
WP_106019735.1\|1139252_1140611_-	YncE family protein	NA	NA	NA	NA
WP_151174592.1\|1140839_1141961_+	tetratricopeptide repeat protein	A0A1P8CWN8	Bacillus_phage	8.0e-81	44.0
WP_024123223.1\|1141967_1142087_+	PhrC/PhrF family phosphatase-inhibitory pheromone	NA	NA	NA	NA
WP_095712788.1\|1142193_1143066_-	agmatinase	NA	NA	NA	NA
WP_064815948.1\|1143126_1143957_-	spermidine synthase	NA	NA	NA	NA
WP_106019737.1\|1144157_1146233_+	penicillin-binding protein	NA	NA	NA	NA
WP_024123227.1\|1146841_1147360_-	hypothetical protein	NA	NA	NA	NA
WP_151174593.1\|1147372_1148032_-\|protease	site-2 protease family protein	NA	NA	NA	NA
WP_003222038.1\|1148140_1148329_+	4-oxalocrotonate tautomerase	NA	NA	NA	NA
WP_024123229.1\|1148371_1148791_-	MarR family transcriptional regulator	NA	NA	NA	NA
WP_151174594.1\|1148909_1150826_-\|tRNA	threonine--tRNA ligase	A0A2K9L6B6	Tupanvirus	1.3e-144	41.4
WP_151174595.1\|1151671_1153069_-	MFS transporter	NA	NA	NA	NA
WP_106019741.1\|1153065_1153539_-	Rrf2 family transcriptional regulator	NA	NA	NA	NA
WP_024123233.1\|1153650_1154151_-	YwgA family protein	NA	NA	NA	NA
WP_059335166.1\|1154187_1155489_-	HD domain-containing protein	E3T4P8	Cafeteria_roenbergensis_virus	5.5e-25	29.3
WP_024123235.1\|1155650_1155875_-	DUF1450 domain-containing protein	NA	NA	NA	NA
WP_024123236.1\|1156089_1156866_+	RsfA family transcriptional regulator	A0A1D6X8E5	Bacillus_phage	6.5e-05	34.5
WP_095712780.1\|1157006_1157897_-	DMT family transporter	NA	NA	NA	NA
WP_024123239.1\|1158046_1158892_-	lipoate--protein ligase family protein	NA	NA	NA	NA
WP_106019742.1\|1158939_1159839_-	cysJI operon transcriptional regulator CysL	A0A2H4J8I9	uncultured_Caudovirales_phage	3.6e-07	42.5
WP_095712778.1\|1159968_1160940_-	phosphate acetyltransferase	NA	NA	NA	NA
WP_003235942.1\|1161209_1161974_+	heme-dependent peroxidase	NA	NA	NA	NA
WP_105954423.1\|1162106_1162886_+	NADPH-dependent reductase BacG	NA	NA	NA	NA
WP_024123243.1\|1162900_1164100_-	pyridoxal phosphate-dependent aminotransferase	NA	NA	NA	NA
WP_095712776.1\|1165288_1166707_-	ATP-grasp domain-containing protein	NA	NA	NA	NA
WP_024123246.1\|1166729_1167491_-	dihydroanticapsin 7-dehydrogenase	Q06VL0	Trichoplusia_ni_ascovirus	3.7e-21	32.0
WP_106019744.1\|1167490_1168201_-	cupin domain-containing protein	NA	NA	NA	NA
WP_095712765.1\|1168190_1168805_-	bacilysin biosynthesis protein BacA	NA	NA	NA	NA
WP_106021905.1\|1170869_1172282_-	amino acid permease	NA	NA	NA	NA
WP_106021904.1\|1172281_1173982_-	M20/M25/M40 family metallo-hydrolase	NA	NA	NA	NA
WP_127696368.1\|1174053_1175601_-	L-glutamate gamma-semialdehyde dehydrogenase	NA	NA	NA	NA
WP_024123255.1\|1175831_1177106_-	glutamate dehydrogenase	NA	NA	NA	NA
WP_024123256.1\|1177288_1177753_-	biofilm-surface layer protein BslB	NA	NA	NA	NA
WP_010332322.1\|1178080_1178536_-\|coat	spore coat protein	NA	NA	NA	NA
WP_095712760.1\|1178528_1179380_-	dTDP-4-dehydrorhamnose reductase	A0A291LA50	Escherichia_phage	1.5e-39	40.1
WP_024123258.1\|1179393_1180341_-	dTDP-glucose 4,6-dehydratase	I7HTA3	Enterobacteria_phage	2.2e-71	43.5
WP_106019749.1\|1180343_1181081_-	NTP transferase domain-containing protein	I7I009	Enterobacteria_phage	4.5e-48	41.9
WP_106019750.1\|1181105_1182125_-\|coat	spore coat protein	NA	NA	NA	NA
WP_106019751.1\|1182126_1182867_-\|coat	spore coat protein	NA	NA	NA	NA

DBSCAN-SWA_3

1833051 : 1841036

Caulobacter_phage(50.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_024120122.1\|1833051_1833747_+	zinc ABC transporter ATP-binding protein ZnuC	G3M9Y6	Bacillus_virus	6.0e-18	28.1
WP_024120123.1\|1833704_1834547_+	zinc ABC transporter permease ZnuB	NA	NA	NA	NA
WP_151174732.1\|1834587_1835583_-	MsnO8 family LLM class oxidoreductase	NA	NA	NA	NA
WP_010332925.1\|1835870_1836470_+	TerD family protein	K4JRX3	Caulobacter_phage	9.7e-25	31.5
WP_010332926.1\|1836492_1837074_+	TerD family protein	K4JRX3	Caulobacter_phage	3.3e-30	40.4
WP_010332927.1\|1837109_1837688_+	TerD family protein	K4JRX3	Caulobacter_phage	6.2e-29	38.0
WP_106022463.1\|1837739_1838513_+	TerC family protein	S5MAL1	Bacillus_phage	1.0e-82	64.8
WP_151174733.1\|1838575_1839736_+	HpcH/HpaI aldolase/citrate lyase family protein	NA	NA	NA	NA
WP_151175189.1\|1839725_1841036_+	hypothetical protein	A0A172Q0Y1	Acinetobacter_phage	2.1e-08	27.4

DBSCAN-SWA_4

2782012 : 2815904

Planktothrix_phage(20.0%)

coat,tRNA

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_010333741.1\|2782012_2783005_-\|tRNA	tryptophan--tRNA ligase	NA	NA	NA	NA
WP_024120913.1\|2783745_2785383_+	oligopeptide ABC transporter substrate-binding protein OppA	NA	NA	NA	NA
WP_024120914.1\|2785490_2786426_+	oligopeptide ABC transporter permease OppB	NA	NA	NA	NA
WP_024120915.1\|2786429_2787347_+	oligopeptide ABC transporter permease OppC	NA	NA	NA	NA
WP_081638274.1\|2787351_2788428_+	oligopeptide ABC transporter ATP-binding protein OppD	G9BWD6	Planktothrix_phage	6.0e-17	29.9
WP_106020545.1\|2788429_2789347_+	oligopeptide ABC transporter ATP-binding protein OppF	M1HP82	Acanthocystis_turfacea_Chlorella_virus	5.3e-06	25.1
WP_127695468.1\|2789456_2790674_+	MFS transporter	NA	NA	NA	NA
WP_010333747.1\|2790839_2791418_+	GNAT family N-acetyltransferase	NA	NA	NA	NA
WP_024120919.1\|2791597_2791993_+	transcriptional regulator Spx	NA	NA	NA	NA
WP_044156385.1\|2792035_2792692_-	TerC family protein	W8EBD0	Pseudomonas_phage	1.9e-29	40.0
WP_120698858.1\|2792878_2793004_+	hypothetical protein	NA	NA	NA	NA
WP_127695466.1\|2792970_2793627_+	adaptor protein MecA	NA	NA	NA	NA
WP_151174927.1\|2793784_2794936_+	competence protein CoiA	NA	NA	NA	NA
WP_151174928.1\|2794982_2796995_+	oligoendopeptidase F	NA	NA	NA	NA
WP_024120925.1\|2797030_2797198_-	hypothetical protein	NA	NA	NA	NA
WP_106020541.1\|2797293_2797497_-	hypothetical protein	NA	NA	NA	NA
WP_024120926.1\|2797511_2798411_-	adaptor protein SpxH	NA	NA	NA	NA
WP_010333756.1\|2798407_2798806_-	thiol management oxidoreductase	NA	NA	NA	NA
WP_127696909.1\|2799062_2799605_-	lytic transglycosylase domain-containing protein	A0A1P8CWQ1	Bacillus_phage	1.1e-38	60.4
WP_024120928.1\|2799808_2800381_-	CYTH domain-containing protein	NA	NA	NA	NA
WP_095713617.1\|2800504_2800873_+	hypothetical protein	NA	NA	NA	NA
WP_024120930.1\|2800900_2801536_+	GTP diphosphokinase	NA	NA	NA	NA
WP_151174929.1\|2801555_2802356_+	NAD kinase	NA	NA	NA	NA
WP_151175198.1\|2802418_2803270_+	RluA family pseudouridine synthase	NA	NA	NA	NA
WP_024120932.1\|2803287_2804025_-	bis(5'-nucleosyl)-tetraphosphatase PrpE	M4Q4T6	Vibrio_phage	7.7e-08	23.2
WP_151174930.1\|2806301_2807009_+	thiaminase II	NA	NA	NA	NA
WP_103672303.1\|2806986_2807604_+	thiazole tautomerase TenI	NA	NA	NA	NA
WP_151174931.1\|2807587_2808697_+	glycine oxidase ThiO	NA	NA	NA	NA
WP_081638285.1\|2808696_2808897_+	sulfur carrier protein ThiS	NA	NA	NA	NA
WP_151174932.1\|2808893_2809664_+	thiazole synthase	NA	NA	NA	NA
WP_151174933.1\|2809660_2810671_+	thiazole biosynthesis adenylyltransferase ThiF	NA	NA	NA	NA
WP_151174934.1\|2810689_2811505_+	bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase	NA	NA	NA	NA
WP_024120941.1\|2811641_2812418_+	enoyl-ACP reductase FabI	NA	NA	NA	NA
WP_081638279.1\|2812518_2813244_+\|coat	spore coat protein	NA	NA	NA	NA
WP_095713608.1\|2813330_2813780_-\|coat	spore coat protein CotZ	NA	NA	NA	NA
WP_003239243.1\|2813907_2814396_-\|coat	spore coat protein CotY	NA	NA	NA	NA
WP_127695460.1\|2814546_2815059_-\|coat	spore coat protein	NA	NA	NA	NA
WP_095713606.1\|2815147_2815477_-\|coat	spore coat protein	NA	NA	NA	NA
WP_105953710.1\|2815517_2815904_-\|coat	spore coat protein CotV	NA	NA	NA	NA

DBSCAN-SWA_5

2883219 : 2928227

Bacillus_phage(29.03%)

protease,plate,terminase,holin,tail,portal

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_081638280.1\|2883219_2884356_+	response regulator aspartate phosphatase RapA	A0A1P8CWN8	Bacillus_phage	1.7e-94	47.6
WP_024121047.1\|2884345_2884480_+	phosphatase RapA inhibitor PhrA	NA	NA	NA	NA
WP_044156268.1\|2884521_2884776_-	hypothetical protein	NA	NA	NA	NA
WP_106020465.1\|2884885_2885833_+	LysM peptidoglycan-binding domain-containing protein	A0A218KC88	Bacillus_phage	4.2e-67	73.5
WP_024121050.1\|2886082_2886310_-	hypothetical protein	NA	NA	NA	NA
WP_024121051.1\|2886719_2887343_+	poly-gamma-glutamate hydrolase family protein	F8WQ53	Bacillus_phage	2.2e-43	46.7
WP_095713506.1\|2888277_2888874_-	ImmA/IrrE family metallo-endopeptidase	A0A2H4JA43	uncultured_Caudovirales_phage	2.1e-40	52.3
WP_106022252.1\|2889034_2889376_-	helix-turn-helix transcriptional regulator	A0A2H4J6F9	uncultured_Caudovirales_phage	5.9e-19	46.6
WP_024121055.1\|2889554_2889734_+	hypothetical protein	NA	NA	NA	NA
WP_106022251.1\|2889720_2890557_+\|portal	phage portal protein	S6BFM4	Thermus_phage	1.3e-22	29.4
WP_082687928.1\|2890456_2891257_+	ATP-binding protein	A6XMI1	Bacillus_virus	5.5e-60	50.9
WP_024121058.1\|2891256_2891424_+	hypothetical protein	NA	NA	NA	NA
WP_106022250.1\|2891508_2891868_+\|portal	phage portal protein	NA	NA	NA	NA
WP_010333893.1\|2891854_2892061_+	hypothetical protein	A0A2H4J4M6	uncultured_Caudovirales_phage	9.3e-12	48.5
WP_151174954.1\|2892177_2892687_+	sigma-70 family RNA polymerase sigma factor	A0A1L2JY33	Aeribacillus_phage	8.5e-22	39.2
WP_106020461.1\|2892804_2893596_+\|terminase	terminase	A0A0S2MVB6	Bacillus_phage	1.4e-58	49.0
WP_151174955.1\|2893592_2894894_+\|terminase	PBSX family phage terminase large subunit	A0A2P1JTW5	Anoxybacillus_phage	9.1e-153	60.9
WP_024121064.1\|2894894_2896385_+\|portal	phage portal protein	A0A1B1P7C8	Bacillus_phage	1.6e-140	55.7
WP_024121065.1\|2896404_2897232_+\|portal	phage portal protein	A0A1B1P7E4	Bacillus_phage	1.4e-53	58.5
WP_024121066.1\|2897256_2898192_+	hypothetical protein	A0A1B1P7E3	Bacillus_phage	1.5e-104	63.8
WP_024121067.1\|2898215_2898599_+	DUF3199 family protein	A0A1B1P7D6	Bacillus_phage	5.8e-15	39.2
WP_095713499.1\|2898595_2898952_+	YqbH/XkdH family protein	NA	NA	NA	NA
WP_106022247.1\|2898948_2899437_+	HK97 gp10 family phage protein	A0A249XXA4	Clostridium_phage	4.4e-36	43.0
WP_106020456.1\|2899449_2899890_+\|portal	phage portal protein	NA	NA	NA	NA
WP_059336109.1\|2899892_2900105_+	hypothetical protein	NA	NA	NA	NA
WP_106022246.1\|2900104_2901505_+\|tail	phage tail sheath protein	A0A0A7RTT5	Clostridium_phage	1.9e-79	39.4
WP_003220592.1\|2901506_2901950_+\|tail	phage tail tube protein	A0A0K2CNG3	Brevibacillus_phage	1.1e-25	45.2
WP_003232676.1\|2902039_2902486_+	phage-like element PBSX protein XkdN	A0A249XXA9	Clostridium_phage	7.5e-14	33.6
WP_003239113.1\|2902515_2902665_+	hypothetical protein	NA	NA	NA	NA
WP_151175201.1\|2907357_2908335_+\|portal	phage portal protein	H7BV96	unidentified_phage	2.5e-38	32.3
WP_024121076.1\|2908334_2908601_+	DUF2577 domain-containing protein	S6C459	Thermus_phage	8.4e-05	36.4
WP_024121077.1\|2908659_2909085_+	DUF2634 domain-containing protein	A0A2H4J6K5	uncultured_Caudovirales_phage	6.6e-12	36.6
WP_151174956.1\|2909077_2910124_+\|plate	baseplate J/gp47 family protein	S6AVU3	Thermus_phage	2.7e-70	43.5
WP_151174957.1\|2910107_2910686_+	DUF2313 domain-containing protein	A0A2H4J717	uncultured_Caudovirales_phage	6.7e-15	32.4
WP_095713489.1\|2910682_2910955_+	hypothetical protein	NA	NA	NA	NA
WP_106020448.1\|2913077_2913407_+\|portal	phage portal protein	A0A2H4JCI0	uncultured_Caudovirales_phage	1.9e-14	40.4
WP_106020447.1\|2913403_2913568_+	XkdX family protein	A0A2H4JAA1	uncultured_Caudovirales_phage	1.2e-14	64.2
WP_106020446.1\|2913611_2914451_+	phage-like element PBSX protein XepA	NA	NA	NA	NA
WP_106020445.1\|2914503_2914773_+	protein xhlA	A0A2H4JD40	uncultured_Caudovirales_phage	3.3e-25	65.9
WP_024121086.1\|2914784_2915048_+\|holin	phage holin	A0A2H4J6M0	uncultured_Caudovirales_phage	4.7e-24	63.2
WP_151174958.1\|2915060_2915957_+	LysM peptidoglycan-binding domain-containing protein	Q9ZXD7	Bacillus_phage	2.3e-83	69.2
WP_151174959.1\|2916009_2917125_+	acyltransferase family protein	NA	NA	NA	NA
WP_151174960.1\|2917121_2917274_-	hypothetical protein	NA	NA	NA	NA
WP_024121090.1\|2917357_2917528_-	type II toxin-antitoxin system SpoIISB family antitoxin	NA	NA	NA	NA
WP_024121091.1\|2917527_2918274_-	toxin-antitoxin-antitoxin system toxin SpoIISA	NA	NA	NA	NA
WP_024121092.1\|2918382_2919384_-	inorganic phosphate transporter	NA	NA	NA	NA
WP_010333926.1\|2919396_2920014_-	DUF47 domain-containing protein	NA	NA	NA	NA
WP_099042852.1\|2920290_2921607_-	serine/threonine exchanger	NA	NA	NA	NA
WP_127695396.1\|2922001_2922952_+	ring-cleaving dioxygenase	NA	NA	NA	NA
WP_106020443.1\|2923199_2925374_+	glycosyltransferase family 39 protein	NA	NA	NA	NA
WP_105953633.1\|2925386_2926358_+	glycosyltransferase family 2 protein	A0A2H5BFL1	Salmonella_phage	3.2e-62	41.0
WP_151174961.1\|2926538_2926778_-	hypothetical protein	NA	NA	NA	NA
WP_151174962.1\|2926871_2928227_-\|protease	serine protease HtrA	A0A1B1IT49	uncultured_Mediterranean_phage	7.5e-25	33.7

DBSCAN-SWA_6

3448593 : 3455721

Bacillus_phage(50.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_024121498.1\|3448593_3448986_+	class Ib ribonucleoside-diphosphate reductase assembly flavoprotein NrdI	G3MBF1	Bacillus_virus	1.0e-30	60.0
WP_151175048.1\|3448945_3451048_+	class 1b ribonucleoside-diphosphate reductase subunit alpha	U5PY35	Bacillus_phage	0.0e+00	86.7
WP_044155791.1\|3451065_3452055_+	class 1b ribonucleoside-diphosphate reductase subunit beta	F8WQ21	Bacillus_phage	1.9e-155	83.2
WP_044155790.1\|3452103_3452724_+	hypothetical protein	A0A127AYS1	Bacillus_phage	5.8e-49	50.2
WP_101861257.1\|3452790_3453558_-	N-acetylmuramoyl-L-alanine amidase	E5DV68	Deep-sea_thermophilic_phage	9.7e-54	50.4
WP_049809927.1\|3454752_3455721_+	stage V sporulation protein K	G3MAX6	Bacillus_virus	6.1e-53	42.9

DBSCAN-SWA_7

3882240 : 3888446

Staphylococcus_phage(66.67%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_044154877.1\|3882240_3882834_-	SMC-Scp complex subunit ScpB	A0A1B1IVT7	uncultured_Mediterranean_phage	4.5e-14	34.8
WP_106020336.1\|3882823_3883579_-	segregation/condensation protein A	A0A1B1IVW1	uncultured_Mediterranean_phage	1.4e-07	25.7
WP_024121909.1\|3883793_3883883_+	YjcZ family sporulation protein	NA	NA	NA	NA
WP_127696808.1\|3883970_3884495_+	DUF309 domain-containing protein	NA	NA	NA	NA
WP_003223910.1\|3884508_3884883_-	GNAT family N-acetyltransferase RibT	NA	NA	NA	NA
WP_024121911.1\|3884994_3885459_-	6,7-dimethyl-8-ribityllumazine synthase	A0A2H4PQS3	Staphylococcus_phage	7.2e-44	59.3
WP_151175116.1\|3885491_3886688_-	bifunctional 3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP cyclohydrolase II	A0A2H4PQS2	Staphylococcus_phage	4.1e-115	54.9
WP_024121913.1\|3886702_3887350_-	riboflavin synthase subunit alpha	A0A2H4PQS5	Staphylococcus_phage	1.7e-43	46.4
WP_106022134.1\|3887360_3888446_-	bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD	A0A2H4PQS8	Staphylococcus_phage	2.1e-54	35.3

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

Overview of predicted results

Overview of the results

Cas Category Instructions

Results visualization

1. NZ_CP041357

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Click the colored protein region to show detailed information

Click the colored protein region to show detailed information

Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection