CRISPRimmunity

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Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Target MGE spacer number	Prophage number
NZ_CP043439	Cupriavidus campinensis strain MJ1 plasmid unnamed2, complete sequence	0 crisprs	DEDDh,csa3	0	2
NZ_CP043440	Cupriavidus campinensis strain MJ1 chromosome, complete genome	0 crisprs	DEDDh,csa3,DinG,cas3,WYL	0	1
NZ_CP043441	Cupriavidus campinensis strain MJ1 plasmid unnamed1, complete sequence	1 crisprs	DEDDh,csa3	1	0

Results visualization

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CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

41736 : 90064

Listeria_phage(25.0%)

transposase,protease,integrase

attL 39430:39443|attR 49868:49881

The bacterium proteins that are colored denote the protein is present at specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin' and 'tRNA')

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
39430:39443	attL	NA	NA	NA	NA
WP_011239934.1\|41736_42882_+\|integrase	tyrosine-type recombinase/integrase	A8ATJ6	Listeria_phage	9.5e-05	27.1
WP_011514892.1\|42856_44515_+\|integrase	tyrosine-type recombinase/integrase	NA	NA	NA	NA
WP_149135058.1\|44511_45762_+	hypothetical protein	NA	NA	NA	NA
WP_149135059.1\|45758_46424_+	hypothetical protein	NA	NA	NA	NA
WP_011514894.1\|46420_46921_+	hypothetical protein	NA	NA	NA	NA
WP_029309942.1\|47222_48665_-	hypothetical protein	NA	NA	NA	NA
WP_149135060.1\|54370_55606_-	PAS domain S-box protein	NA	NA	NA	NA
49868:49881	attR	NA	NA	NA	NA
WP_149135220.1\|55696_55960_-	hypothetical protein	NA	NA	NA	NA
WP_149135061.1\|57503_57716_-	hypothetical protein	NA	NA	NA	NA
WP_149135062.1\|57660_58590_-	FCD domain-containing protein	NA	NA	NA	NA
WP_149135063.1\|58570_59149_-	hypothetical protein	NA	NA	NA	NA
WP_149135064.1\|59726_60557_+	alpha/beta fold hydrolase	NA	NA	NA	NA
WP_149135065.1\|62150_62651_+	3-phenylpropionate/cinnamic acid dioxygenase subunit beta	NA	NA	NA	NA
WP_149135066.1\|62660_62984_+	Rieske 2Fe-2S domain-containing protein	NA	NA	NA	NA
WP_149135221.1\|62983_64216_+	FAD-dependent oxidoreductase	NA	NA	NA	NA
WP_149135067.1\|65051_65927_+	biphenyl-2,3-diol 1,2-dioxygenase	NA	NA	NA	NA
WP_149135068.1\|66048_66855_+	2-keto-4-pentenoate hydratase	NA	NA	NA	NA
WP_149135069.1\|66962_67913_+	acetaldehyde dehydrogenase (acetylating)	E5EQ71	Micromonas_sp._RCC1109_virus	4.8e-34	34.4
WP_149135070.1\|69380_69710_+	hypothetical protein	NA	NA	NA	NA
WP_149135071.1\|69622_70087_+	outer membrane beta-barrel protein	NA	NA	NA	NA
WP_149135072.1\|71155_72196_-	aldo/keto reductase	NA	NA	NA	NA
WP_149135073.1\|72293_73310_-	zinc-binding dehydrogenase	NA	NA	NA	NA
WP_149135074.1\|73335_74154_-	carboxymuconolactone decarboxylase	NA	NA	NA	NA
WP_149135075.1\|74110_74635_-	cupin domain-containing protein	NA	NA	NA	NA
WP_149135076.1\|74681_75092_-	cupin domain-containing protein	NA	NA	NA	NA
WP_149135077.1\|75197_76091_+	LysR family transcriptional regulator	NA	NA	NA	NA
WP_149135078.1\|76705_77101_+	hypothetical protein	NA	NA	NA	NA
WP_149135079.1\|77173_78265_+\|transposase	IS5 family transposase	NA	NA	NA	NA
WP_149135080.1\|79850_81065_+	DUF5131 family protein	Q8W6R4	Burkholderia_virus	3.3e-72	47.9
WP_029309168.1\|81241_81508_+	hypothetical protein	NA	NA	NA	NA
WP_149135081.1\|81590_81788_+	hypothetical protein	NA	NA	NA	NA
WP_149135082.1\|81835_82261_+	hypothetical protein	NA	NA	NA	NA
WP_149135083.1\|82581_83193_+	GNAT family N-acetyltransferase	NA	NA	NA	NA
WP_149135084.1\|83189_83498_+	hypothetical protein	NA	NA	NA	NA
WP_149135085.1\|83532_84267_+	hypothetical protein	NA	NA	NA	NA
WP_149135086.1\|84456_84999_+\|protease	M48 family metalloprotease	NA	NA	NA	NA
WP_149135087.1\|85114_86284_+\|protease	metalloprotease	NA	NA	NA	NA
WP_149135088.1\|86348_86528_-	hypothetical protein	NA	NA	NA	NA
WP_149135089.1\|86524_86797_-	hypothetical protein	NA	NA	NA	NA
WP_149135090.1\|87232_87658_+	hypothetical protein	NA	NA	NA	NA
WP_149135091.1\|87675_88032_+	hypothetical protein	NA	NA	NA	NA
WP_149135092.1\|88044_88662_+	hypothetical protein	NA	NA	NA	NA
WP_149135093.1\|88880_90064_-\|transposase	IS3 family transposase	A0A0P0I4A4	Acinetobacter_phage	3.3e-61	43.5

DBSCAN-SWA_2

159406 : 215372

Acinetobacter_phage(28.57%)

transposase,protease,integrase

attL 209906:209921|attR 222868:222883

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_149135079.1\|159406_160498_-\|transposase	IS5 family transposase	NA	NA	NA	NA
WP_149135155.1\|160695_161511_+	hypothetical protein	NA	NA	NA	NA
WP_149135156.1\|162052_162640_+	hypothetical protein	NA	NA	NA	NA
WP_149135157.1\|165112_165628_+	hypothetical protein	NA	NA	NA	NA
WP_149135158.1\|166419_169038_+	carboxypeptidase regulatory-like domain-containing protein	NA	NA	NA	NA
WP_149135159.1\|169355_170186_+	hypothetical protein	NA	NA	NA	NA
WP_149135160.1\|170514_171528_+	hypothetical protein	NA	NA	NA	NA
WP_149135161.1\|171739_172393_+	hypothetical protein	I6NP76	Burkholderia_phage	9.9e-07	37.6
WP_149135162.1\|172431_173262_+	hypothetical protein	NA	NA	NA	NA
WP_149135163.1\|173478_175272_+	phosphoadenosine phosphosulfate sulfotransferase	NA	NA	NA	NA
WP_149135164.1\|175551_175980_+	hypothetical protein	NA	NA	NA	NA
WP_149135165.1\|176172_176628_+	hypothetical protein	NA	NA	NA	NA
WP_149135166.1\|176812_177292_+	hypothetical protein	NA	NA	NA	NA
WP_149135167.1\|177354_177747_+	hypothetical protein	NA	NA	NA	NA
WP_149135168.1\|178019_178859_+\|protease	cysteine protease	NA	NA	NA	NA
WP_149135093.1\|179234_180417_+\|transposase	IS3 family transposase	A0A0P0I4A4	Acinetobacter_phage	3.3e-61	43.5
WP_149135169.1\|180661_181036_+	hypothetical protein	NA	NA	NA	NA
WP_149135170.1\|181105_181915_-	HNH endonuclease	NA	NA	NA	NA
WP_149135171.1\|182377_183511_+	hypothetical protein	NA	NA	NA	NA
WP_149135172.1\|183690_184527_-	hypothetical protein	NA	NA	NA	NA
WP_149135173.1\|184472_185624_-	DnaJ domain-containing protein	L7RC83	Acanthamoeba_polyphaga_moumouvirus	2.2e-09	28.6
WP_149135174.1\|185784_186087_-	hypothetical protein	NA	NA	NA	NA
WP_149135175.1\|186130_186661_+	hypothetical protein	NA	NA	NA	NA
WP_003049965.1\|186805_189721_-\|transposase	Tn3-like element IS1071 family transposase	A0A1B0V7H9	Salmonella_phage	1.4e-52	24.0
WP_149135176.1\|189835_190285_-	hypothetical protein	NA	NA	NA	NA
WP_077176980.1\|190281_190989_-	c-type cytochrome	NA	NA	NA	NA
WP_077176981.1\|190998_191463_-	hypothetical protein	NA	NA	NA	NA
WP_077176982.1\|191482_191935_-	hypothetical protein	NA	NA	NA	NA
WP_143274053.1\|191945_192749_-	DUF2243 domain-containing protein	NA	NA	NA	NA
WP_077176984.1\|192869_193469_-	YeeE/YedE family protein	NA	NA	NA	NA
WP_077176985.1\|193468_193996_-	YeeE/YedE family protein	NA	NA	NA	NA
WP_077176987.1\|195583_196018_-	hypothetical protein	NA	NA	NA	NA
WP_077176988.1\|196027_196462_-	YeeE/YedE family protein	NA	NA	NA	NA
WP_077176989.1\|196502_197978_-	multicopper oxidase domain-containing protein	NA	NA	NA	NA
WP_077176990.1\|197974_198307_-	helix-turn-helix transcriptional regulator	NA	NA	NA	NA
WP_077176991.1\|199432_199786_+	TIGR01244 family phosphatase	NA	NA	NA	NA
WP_077176992.1\|199782_201534_+	sulfate permease	A0A2H4J153	uncultured_Caudovirales_phage	1.7e-13	22.8
WP_077176993.1\|201530_202280_+	MBL fold metallo-hydrolase	NA	NA	NA	NA
WP_077176994.1\|202396_202804_+	DUF1232 domain-containing protein	NA	NA	NA	NA
WP_077198917.1\|202850_203201_+	hypothetical protein	NA	NA	NA	NA
WP_077176996.1\|203314_203761_+	hypothetical protein	NA	NA	NA	NA
WP_149135093.1\|204838_206021_+\|transposase	IS3 family transposase	A0A0P0I4A4	Acinetobacter_phage	3.3e-61	43.5
WP_005793020.1\|207333_208137_-	phosphodiesterase	NA	NA	NA	NA
WP_100084379.1\|208160_209432_-	ABC transporter substrate-binding protein	NA	NA	NA	NA
WP_005793022.1\|209428_210271_-	carbohydrate ABC transporter permease	NA	NA	NA	NA
209906:209921	attL	NA	NA	NA	NA
WP_005793023.1\|210267_211140_-	sugar ABC transporter permease	NA	NA	NA	NA
WP_005793024.1\|211142_212228_-	ABC transporter ATP-binding protein	Q6GZ03	Mycoplasma_phage	2.0e-20	50.5
WP_005793025.1\|212233_212890_-	HAD family hydrolase	NA	NA	NA	NA
WP_100084380.1\|212991_213792_+	DeoR/GlpR transcriptional regulator	NA	NA	NA	NA
WP_005793027.1\|214280_215372_+\|integrase	site-specific integrase	NA	NA	NA	NA
222868:222883	attR	NA	NA	NA	NA

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

2. NZ_CP043440

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CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

3058580 : 3064527

uncultured_Caudovirales_phage(33.33%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_149137125.1\|3058580_3059162_-	GNAT family N-acetyltransferase	A0A2H4J136	uncultured_Caudovirales_phage	2.2e-21	39.5
WP_149137126.1\|3059260_3059620_+	arsenate reductase (glutaredoxin)	A0A2H4J8T1	uncultured_Caudovirales_phage	4.4e-17	44.4
WP_149137127.1\|3059717_3059945_-	hypothetical protein	NA	NA	NA	NA
WP_149137128.1\|3060050_3060722_-	exonuclease	A0A2L0UZL4	Agrobacterium_phage	1.0e-35	39.5
WP_149137129.1\|3060743_3061394_-	MBL fold metallo-hydrolase	NA	NA	NA	NA
WP_149137628.1\|3061813_3062479_+	septal ring lytic transglycosylase RlpA family protein	H2BCY4	Synechococcus_phage	3.9e-19	55.3
WP_149137130.1\|3062556_3063492_-	16S rRNA (cytidine(1402)-2'-O)-methyltransferase	M1PLC5	Streptococcus_phage	1.9e-35	38.9
WP_149137131.1\|3063507_3063909_+	YraN family protein	NA	NA	NA	NA
WP_149137132.1\|3063939_3064527_+	phosphoheptose isomerase	A0A067XQR2	Caulobacter_phage	8.9e-15	32.0

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

3. NZ_CP043441

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Crispr_ID: NZ_CP043441_1

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

NZ_CP043441_1

1595147-1595225

Orphan

Consensus_repeat	Method
CAAAGCGTACCCGTTTCTGCGCG	CRISPRCasFinder

1 spacers

The CRISPR arrays of NZ_CP043441_1

>merge|NZ_CP043441|1|1595147-1595225|CRISPRCasFinder
CAAAGCGTACCCGTTTCTGCGCGGCTTTTTCCGCTGCCTGATGCCGCCCCCGCCGGCAAAGCGTACCCATTTCTGCGCG

>NZ_CP043441|1|1|1595147-1595225|CRISPRCasFinder
CAAAGCGTACCCGTTTCTGCGCG	GCTTTTTCCGCTGCCTGATGCCGCCCCCGCCGG
CAAAGCGTACCCATTTCTGCGCG

Protein	Signature genes	Signature genes Name	Protein_function
NZ_CP043441.1\|WP_149138523.1\|1582078_1583494_+\|heavy-metal-sensor-histidine-kinase	unknown	unknown	gnl\|CDD\|273593
NZ_CP043441.1\|WP_149138529.1\|1591855_1593271_+\|class-II-fumarate-hydratase	unknown	unknown	gnl\|CDD\|234779
NZ_CP043441.1\|WP_149138537.1\|1601976_1603815_+\|phosphogluconate-dehydratase	unknown	unknown	gnl\|CDD\|181628
NZ_CP043441.1\|WP_149138538.1\|1603811_1604756_-\|mechanosensitive-ion-channel	unknown	unknown	gnl\|CDD\|223740
NZ_CP043441.1\|WP_149138539.1\|1605059_1606340_-\|MFS-transporter	unknown	unknown	gnl\|CDD\|340863
NZ_CP043441.1\|WP_149138535.1\|1599485_1600166_-\|6-phosphogluconolactonase	unknown	unknown	gnl\|CDD\|238694
NZ_CP043441.1\|WP_149138525.1\|1584526_1584937_+\|hypothetical-protein	unknown	unknown	gnl\|CDD\|237865
NZ_CP043441.1\|WP_144201011.1\|1586417_1586744_+\|PadR-family-transcriptional-regulator	unknown	unknown	gnl\|CDD\|224609
NZ_CP043441.1\|WP_149138524.1\|1583954_1584410_+\|ferric-iron-uptake-transcriptional-regulator	unknown	unknown	gnl\|CDD\|236527
NZ_CP043441.1\|WP_149138533.1\|1597667_1598375_-\|response-regulator	unknown	unknown	gnl\|CDD\|223816
NZ_CP043441.1\|WP_149138527.1\|1586882_1588376_-\|EAL-domain-containing-protein	unknown	unknown	gnl\|CDD\|236645
NZ_CP043441.1\|WP_092294150.1\|1590779_1591151_-\|hypothetical-protein	unknown	unknown	unknown
NZ_CP043441.1\|WP_149138536.1\|1600199_1601648_-\|glucose-6-phosphate-dehydrogenase	unknown	unknown	gnl\|CDD\|235579
NZ_CP043441.1\|WP_149138526.1\|1584945_1585857_-\|EamA-family-transporter	unknown	unknown	gnl\|CDD\|273359
NZ_CP043441.1\|WP_149138532.1\|1596303_1597668_-\|HAMP-domain-containing-protein	unknown	unknown	gnl\|CDD\|182388
NZ_CP043441.1\|WP_149138534.1\|1598390_1599413_-\|glucokinase	unknown	unknown	gnl\|CDD\|234716
NZ_CP043441.1\|WP_149138528.1\|1591369_1591720_+\|four-helix-bundle-copper-binding-protein	unknown	unknown	gnl\|CDD\|153434
NZ_CP043441.1\|WP_149138531.1\|1595324_1596269_+\|2-hydroxyacid-dehydrogenase	unknown	unknown	gnl\|CDD\|240633
NZ_CP043441.1\|WP_149138530.1\|1593278_1595096_-\|peptide-transporter	unknown	unknown	gnl\|CDD\|367370
NZ_CP043441.1\|WP_149138540.1\|1606541_1608680_+\|TonB-dependent-siderophore-receptor	unknown	unknown	gnl\|CDD\|227113

Protein	Function_ID	Function_description	E-value
NZ_CP043441.1\|WP_149138523.1\|1582078_1583494_+\|heavy-metal-sensor-histidine-kinase	gnl\|CDD\|273593	TIGR01386, Probable_sensor_protein_PcoS, heavy metal sensor kinase. Members of this family contain a sensor histidine kinase domain (pfam00512) and a domain found in bacterial signal proteins (pfam00672). This group is separated phylogenetically from related proteins with similar architecture and contains a number of proteins associated with heavy metal resistance efflux systems for copper, silver, cadmium, and/or zinc.	1.31214e-119
NZ_CP043441.1\|WP_149138529.1\|1591855_1593271_+\|class-II-fumarate-hydratase	gnl\|CDD\|234779	PRK00485, fumC, fumarate hydratase; Reviewed.	0
NZ_CP043441.1\|WP_149138537.1\|1601976_1603815_+\|phosphogluconate-dehydratase	gnl\|CDD\|181628	PRK09054, PRK09054, phosphogluconate dehydratase; Validated.	0
NZ_CP043441.1\|WP_149138539.1\|1605059_1606340_-\|MFS-transporter	gnl\|CDD\|340863	cd06173, MFS_MefA_like, Macrolide efflux protein A and similar proteins of the Major Facilitator Superfamily of transporters. This family is composed of Streptococcus pyogenes macrolide efflux protein A (MefA) and similar transporters, many of which remain uncharacterized. Some members may be multidrug resistance (MDR) transporters, which are drug/H+ antiporters (DHAs) that mediate the efflux of a variety of drugs and toxic compounds, conferring resistance to these compounds. MefA confers resistance to 14-membered macrolides including erythromycin and to 15-membered macrolides. It functions as an efflux pump to regulate intracellular macrolide levels. The MefA-like family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.	1.92562e-81
NZ_CP043441.1\|WP_149138535.1\|1599485_1600166_-\|6-phosphogluconolactonase	gnl\|CDD\|238694	cd01400, 6PGL, 6PGL: 6-Phosphogluconolactonase (6PGL) subfamily; 6PGL catalyzes the second step of the oxidative phase of the pentose phosphate pathway, the hydrolyzation of 6-phosphoglucono-1,5-lactone (delta form) to 6-phosphogluconate. 6PGL is thought to guard against the accumulation of the delta form of the lactone, which may be toxic through its reaction with endogenous cellular nucleophiles.	4.17572e-61
NZ_CP043441.1\|WP_149138525.1\|1584526_1584937_+\|hypothetical-protein	gnl\|CDD\|237865	PRK14951, PRK14951, DNA polymerase III subunits gamma and tau; Provisional.	0.000499094
NZ_CP043441.1\|WP_144201011.1\|1586417_1586744_+\|PadR-family-transcriptional-regulator	gnl\|CDD\|224609	COG1695, COG1695, Predicted transcriptional regulators [Transcription].	1.27793e-06
NZ_CP043441.1\|WP_149138524.1\|1583954_1584410_+\|ferric-iron-uptake-transcriptional-regulator	gnl\|CDD\|236527	PRK09462, fur, ferric uptake regulator; Provisional.	2.62546e-48
NZ_CP043441.1\|WP_149138533.1\|1597667_1598375_-\|response-regulator	gnl\|CDD\|223816	COG0745, OmpR, Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain [Signal transduction mechanisms / Transcription].	9.72841e-79
NZ_CP043441.1\|WP_149138527.1\|1586882_1588376_-\|EAL-domain-containing-protein	gnl\|CDD\|236645	PRK10060, PRK10060, cyclic di-GMP phosphodiesterase.	1.60863e-100
NZ_CP043441.1\|WP_149138534.1\|1598390_1599413_-\|glucokinase	gnl\|CDD\|234716	PRK00292, glk, glucokinase; Provisional.	7.64161e-132
NZ_CP043441.1\|WP_149138536.1\|1600199_1601648_-\|glucose-6-phosphate-dehydrogenase	gnl\|CDD\|235579	PRK05722, PRK05722, glucose-6-phosphate 1-dehydrogenase; Validated.	0
NZ_CP043441.1\|WP_149138526.1\|1584945_1585857_-\|EamA-family-transporter	gnl\|CDD\|273359	TIGR00950, Uncharacterized_inner_membrane_transporter_YicL, Carboxylate/Amino Acid/Amine Transporter. [Transport and binding proteins, Amino acids, peptides and amines].	1.88791e-19
NZ_CP043441.1\|WP_149138532.1\|1596303_1597668_-\|HAMP-domain-containing-protein	gnl\|CDD\|182388	PRK10337, PRK10337, sensor protein QseC; Provisional.	8.74421e-64
NZ_CP043441.1\|WP_149138538.1\|1603811_1604756_-\|mechanosensitive-ion-channel	gnl\|CDD\|223740	COG0668, MscS, Small-conductance mechanosensitive channel [Cell envelope biogenesis, outer membrane].	1.84694e-53
NZ_CP043441.1\|WP_149138528.1\|1591369_1591720_+\|four-helix-bundle-copper-binding-protein	gnl\|CDD\|153434	cd08026, DUF326, Cysteine-rich 4 helical bundle widely conserved in bacteria. This functionally uncharacterized protein forms a 4-helical bundle with a bromodomain-like topology. It is present in major bacterial lineages and contains highly conserved cysteines in a repeated pattern, whose sidechains appear buried. Some family members have been (mis)annotated as putative ferredoxins.	1.82265e-28
NZ_CP043441.1\|WP_149138531.1\|1595324_1596269_+\|2-hydroxyacid-dehydrogenase	gnl\|CDD\|240633	cd12156, HPPR, Hydroxy(phenyl)pyruvate Reductase, D-isomer-specific 2-hydroxyacid-related dehydrogenase. Hydroxy(phenyl)pyruvate reductase (HPPR) catalyzes the NADP-dependent reduction of hydroxyphenylpyruvates, hydroxypyruvate, or pyruvate to its respective lactate. HPPR acts as a dimer and is related to D-isomer-specific 2-hydroxyacid dehydrogenases, a superfamily that includes groups such as formate dehydrogenase, glycerate dehydrogenase, L-alanine dehydrogenase, and S-Adenosylhomocysteine Hydrolase. Despite often low sequence identity, these proteins typically have a characteristic arrangement of 2 similar subdomains of the alpha/beta Rossmann fold NAD+ binding form. The NAD+ binding domain is inserted within the linear sequence of the mostly N-terminal catalytic domain, which has a similar domain structure to the internal NAD binding domain. Structurally, these domains are connected by extended alpha helices and create a cleft in which NAD is bound, primarily to the C-terminal portion of the 2nd (internal) domain. Some related proteins have similar structural subdomain but with a tandem arrangement of the catalytic and NAD-binding subdomains in the linear sequence. While many members of this family are dimeric, alanine DH is hexameric and phosphoglycerate DH is tetrameric.	1.99086e-138
NZ_CP043441.1\|WP_149138530.1\|1593278_1595096_-\|peptide-transporter	gnl\|CDD\|367370	pfam03169, OPT, OPT oligopeptide transporter protein. The OPT family of oligopeptide transporters is distinct from the ABC pfam00005 and PTR pfam00854 transporter families. OPT transporters were first recognized in fungi (Candida albicans and Schizosaccharomyces pombe), but this alignment also includes orthologues from Arabidopsis thaliana. OPT transporters are thought to have 12-14 transmembrane domains and contain the following motif: SPYxEVRxxVxxxDDP.	1.09427e-26
NZ_CP043441.1\|WP_149138540.1\|1606541_1608680_+\|TonB-dependent-siderophore-receptor	gnl\|CDD\|227113	COG4772, FecA, Outer membrane receptor for Fe3+-dicitrate [Inorganic ion transport and metabolism].	0

>NZ_CP043441.1|WP_149138530.1|1593278_1595096_-|peptide-transporter
MSTLLTPPRWRWLPAPDTWQYHALLGAAGMLVLGPLGCVAAAYMNFSLGFFIGGQVLAGILGSAVTAGYGAAGRHGANYIQTAAASVAGMGGMVVVIQAMGWMGMGAPPLWHLIGYMLCIGMYGVGVGMLYTPLLVDRMQLTFPSGLAVANILRALTDPVLLRRSVARLGGGIALGAVAGVGVTRVAWLGAIDLSASTFGAGMIVGARIGIAALVGGLAGWALTPYFVSIGWLAAGDPYRKITFLIALGMIMGAALVDVSLLLARAWRERLPATPLPAPAVPVPASPVPAAQRGLPMRWIWLWVVVWGAAVVATGAAWFDQPVGYQLLAVALVLVFALVNGISVGMVDANPISSAFVLTVILMAAVGLREPHIGLIAATVLLVSTAEACDMQQDRSTGWRLGTNRMTQFGYQVAGIVVGAVLAVLLARLFMQAYPVLRLDQTLLPADQQPARWTSAMTYKIVGALRSMTEDRPYQRLAVWIGVGIGLVTEVARKAIRASARYRRFAQAGGAGKTVDFLLDAVVLPSPYASSFGGFVNLPAAAWMAAGGVLASVVNSVAKKRGAPARDAALPEDMSTTSLVGGGLIAGDALAALALGVAGLVTVVG
>NZ_CP043441.1|WP_149138529.1|1591855_1593271_+|class-II-fumarate-hydratase
MSAKPANPSSATRIEKDSLGDVAVPADHLWGAQTERSRQNFRIGSEKMPPALIEAFAVLKLCAARANRDLGVLKPEIAEAIEQAAEEVIAGKWPAEFPLSVWQTGSGTQTNMNLNEVISNRAIQLLGGEVGSKKPVHPNDHVNASQSSNDSFPTAMHIAATRAIQQTLLPSLEKLQQTFAKKVEAFADIVKVGRTHLQDAVPLTLGQEFSGYMTQVADAQSRLQQAMLRAMPVAQGGTAVGTGLNAPPGFAAAFARSLAGYTGLPFEPAANRFALQAAHDALADLSGALNTTASSFLKIARDFMLLGSGPRAGFAELQLPANEPGSSIMPGKVNPTQAEALAMVCCRIIGNHTTVTLANSLGTLELNAYKPVIVYSLLQSVTLLADAASSFAEHMVEGVEPDRARIQELLERSLMSVTALNPHIGYDKAAEIAKLAVKKNLSLREAAIASGHVTDEQFGQWIDFKGMTKEV
>NZ_CP043441.1|WP_149138528.1|1591369_1591720_+|four-helix-bundle-copper-binding-protein
MIRPTVRENFDRYADCIAACNACAAACLKCAAACLEEQDARKMTRCIALDMDCAGICTLAASYMLRNSEFAPLVCEDCAEVCKWCKEECERHDHWHCQECAAACQACMEMCLKMTA
>NZ_CP043441.1|WP_092294150.1|1590779_1591151_-|hypothetical-protein
MGEAKRRGTPEERAQQAQARIDALRPAQLVCGACKTSFTNFDAIPAPGLRGIDAVFGGECPSCGNDVVVFKGEPQAVAEAMLAYERMMGESARLGYQSADGRHVPFDEAAPQASDASDDSTKH
>NZ_CP043441.1|WP_149138527.1|1586882_1588376_-|EAL-domain-containing-protein
MTLSTWLPVLSWLLVAAGGALVGWAARAWWSRRASGQVSAAQATQDQTERRRVDEYIRHLAQHDALTGLLNRAALHSRTEAVLDDARRQGGRVALLLLDLDHFKHINETLGHPVGDDVLRTIAQRLRGTVQPRDVVARMGGDEFAVVLGGLRHDSEAELTAAKILARVSEGLQLAGHLAGQPLRVTPSLGMAVYPDDGETLTDLLKSADSAMYAAKRGGRAQLRRFASEMAEASLTRFTIEALLRRALAEQEFRLRYQPIVDLATLNVVGVEALIAWETPERGVMQPSEFIPIAEQCGMIDALGEWTLATACREIQTLRTELGRDIDVAVNISPLQLRRADFPETVARCLQAASLPAPSLTIEVTEGILVDGGETTIESFRRVRQLGVSVSIDDFGTGYSGLGYLTRLPISKLKIDKSFVDDLAMPGHDRAVAAAIIALGHQLHLHVVAEGVESMAQLEFLRGEGCDAVQGFLFSQPLGLSALRDLLLAGNGFSRVA
>NZ_CP043441.1|WP_144201011.1|1586417_1586744_+|PadR-family-transcriptional-regulator
MKRQFLGLLARVYVLQFAMEVPATVAMLAEMLMEYGFQVDIGTVRPLLRALQMDGYLESVLRDGIGRVYQLTEQGREELASSRLRIDELYRRLHSPNGEDAAASTVNG
>NZ_CP043441.1|WP_149138526.1|1584945_1585857_-|EamA-family-transporter
MKPSDLIRLLSLSAIWGASFLFVRIGAPVLGPLPTAFFRVLIGAVTLAGCLMLMKVKWDMRGKWRPVIALGIINSGIPFVMYSVAALWLPAGYSAIFNAMTPLMGVLIGSLFFAEKLTRAKALGVLIGLAGVAVLTRTGPVVFSHDVLLGALACLVATSCYGLAGFLTRRWITARGGLDNRLVAAGSMVGATLFLLPFSLAALVRENTLPGAGLGVWAAMFGLGMVCTAIAYVLYFRLVADLGPVRSLTVTFLIPPFGIVWGWMFLGEALSWAHAAGMLLIGAAVWLVLKPAAPAVKPAAASS
>NZ_CP043441.1|WP_149138525.1|1584526_1584937_+|hypothetical-protein
MNKRPLSFLVFVAGAAALAASTAAMARVDIDVNLGAPAPVYVAPAPVYVEPRPAPVYVAPPPVAYRPAPVVVAPVVIGWHGDRYWDGGRWYDRRQYQAWHRHDNGHGHGYGRDRYDDHRGHDDHGDRGDRGDHHRH
>NZ_CP043441.1|WP_149138524.1|1583954_1584410_+|ferric-iron-uptake-transcriptional-regulator
MPLSSVKSSPTPLQRAGLKATSPRMHVLEAFRRSDRRHLSAEDVYRILLAQEVDAGLSTVYRVLNQLVQANVLLRRTFEADHAVFELNEGGHHDHLICVSCGRVEEFRDEGIEQRQRKVAAENDFELREHMLVLYGLCPACQAVPGSESSI
>NZ_CP043441.1|WP_149138523.1|1582078_1583494_+|heavy-metal-sensor-histidine-kinase
MKTRSLTARLALAFALIAGVSFGGVGIYLYRALATQIIERDNAELLRKATRAGHALAELSPSDQPGSPAWNEILGVVKGNDEYGMRVRAADGALLAANGANYGIRPEHPDLASETGPDMFPVAMWNTPGGTPVRLITLQVRAGVVAAPVTLEIFQLASSRVALLRAYRWKVFAAACLGALGAGILGFAALRAGMAPLRRIAAGTESITFTSGTLPIDPSTLPAELDDLAGALQKMIDRLRERYDRLSHFSADLAHDFRTPIGNLLGQTQVALGSDRSADEYQSLLASNVEEYERLSRMIENMLFLARADNARVALHLAELDLKDELLRQADYFELLAEARAVTIRVDAHGRVFADATLLRRALGNLLANAVRYAHQGSEILVRGRAGASHAAIEITNAGPGIDPAHLPLLFDRFFRADPSRANSVESSGIGLAIVKTIMDLHQGAVSVQSEPEALTSFRLEFPDRHSSPLA
>NZ_CP043441.1|WP_149138531.1|1595324_1596269_+|2-hydroxyacid-dehydrogenase
MKPALPALLVLIQLEPAHLELVSAQYDVRYAPTAEGRAAEVAAHGKAFRAVLTNGTVGLTATEIAAMPALEVVCALGAGYENIDLAACRARGIAVGNGAGTNDSCVADHAMALLLATVRRVPALDHATRDGVWRNALPLPPNFSGKRLGIVGLGTIGRRIAQRGQGFDLEIGYHNRRARPDVSFSYFADVGALAKWADYLVIATPGGPETRHLVDTPVLRALGPHGYLVNIARGSVVNTAALADALRAGELGGAGLDVYESEPLPPKELFDCPNVVLTPHMAGWSPEAVLASVSQFLENTRRHFAGEPLFAPVG
>NZ_CP043441.1|WP_149138532.1|1596303_1597668_-|HAMP-domain-containing-protein
MRSIQRTLLWWLAAGLAAGIAVATGLIYAQARQEANALFDYQMKQVAAALPSQFPSPIAPPFDGGNGSGASADLLHADEDVVIHIWDGTGRSLYLSHSHPVLPPRAELGFSSVRTDSGEWRIYSVQLGPAVVQIAQPMSARRTLAAHMALRTVAPLLLLLPLLAWLIWMAVGRGLRPLREIAAEVRARDANTLAPLALRQMPDEIAPLSDALNQLLARLAQAIDTQRAFVADAAHALRTPLAALQLQAQLVERADSAPARQEAVSQLRQGLERLTHLVAQLLTLARQEPGAAPPPHEPVDLRALAAGVIAEMSQAAIDRDIDLGLESDDAEAETGPATVRGDADALRILLTNLIDNALHYIPAGGRIDVRVASLPGVGSEGGVELQVSDNGPGIPAAERTRVFDRFYRVPEAPTGGSGLGLAIVAEIAQSHAARVALEDAAPGLRVRVTFPVAA
>NZ_CP043441.1|WP_149138533.1|1597667_1598375_-|response-regulator
MRVLLVEDDAMIGESVKVALRQEGFTVDWVRDGDAGLDAASDQHGPRADGGYDLVLLDLGLPRRSGLDVLKTLRARNVRTPVLILTARDAVADRVAGLNAGADDYLVKPFDLQELAARMHALARRAEGRAEPLLRYGDIVLNPATREATQAGTAVRLSAREFALLSALMARPGKVWSVAQLQERLYGWDDEVGSNTVEVYIHALRRKFGAALIRNIRGVGYVVPQRDGADEERPS
>NZ_CP043441.1|WP_149138534.1|1598390_1599413_-|glucokinase
MSPPDAQADSYPRLLADVGGTNVRMALETAPMRIGAVTALKVADFPSLEAAMRQYLRGLHDLPRPRHAAIGLANPVTGDHVKLTNHNWAFSIDATRRALDLDTLVAINDFTALALALPYLPAEDLALVRSGTAVPTAPRALLGPGTGLGVSGLVPAPGGGAVALAGEGGHIEIMPDTDDEWIAWRYAHQHLGRVSAERMLSGMGLSQIHAALAAETGTPLAQPLTPAEVTDGAIKTGDPLCQRAFAAFCGLLGSVAGDVALVLGARGGVYLGGGIVPRFVDAFRASPFAARFAAKGRMGAYLADIPVCVITAAYPALPGLARALADRLEGDMRHGAAAQP
>NZ_CP043441.1|WP_149138535.1|1599485_1600166_-|6-phosphogluconolactonase
MRQYEHASVMDQAESLALSIGHALELVIQAKGWAVLAVSGGRSPVPMFERLRYRPIRWDAVTVTLVDERAVSPDHPDSNGALVRHNLLREAAGVAAWVPLVEDAAEAAQPERAVARLNERFQQPDVVVLGMGEDGHTASLFPEAPELQTALSEPKPGFLVTHPTSANHARVTLNLAALLAAERTFLAISGEKKAEVLARALQAPTPALPVSLVLARHHHGVDVFRT
>NZ_CP043441.1|WP_149138536.1|1600199_1601648_-|glucose-6-phosphate-dehydrogenase
MPDFDMVLFGGTGDLARRKLLPALFDAHRCGVLHPSARILATGSHAQTTEQYIATLEDTVRPALEHAPPDAWASFLQRICYVQADAREPAHFDALAEKVLARKPEVVVCYLATAPHIFVPICEQLGRTGLNTPNVRVVLEKPLGHDLDSSEAINSSVAKFFGEDQIYRIDHYLGKESVQNLMAIRFGNALFEPLWRREWVQEVQITIAEELGVEQRGDFYDRTGALRDMVQNHLLQLLCMVAMEPPASLSEDAIRDEKIKILKALRPIQPQDVAEKTVRGQYRAGAVGGKPVPGYLEEPGIAPDSRTETFVAIKAEIANWRWEGVPFYLRTGKRMQSRVAEIVVHFRDVPHAIFPKPLTLSPQNRLVIQLQPEESIKLYFLVKQPGDTLELTPTSLDLDFANSFKVRRAGAYERLLLDVIRGRLGLFVRRDEQVQAWRWVEPIIDTWANSTVPPKPYTAGTWGPAASSALMSRDNALWHEEV
>NZ_CP043441.1|WP_149138537.1|1601976_1603815_+|phosphogluconate-dehydratase
MPRHPVLERVTARIVTRSRATRQAYLDRTRAMAGQKVERANLSCTNLAHGVAAMPDDAKIRLKAQERPNLAIVSAYNDMLSAHQPLEAFPRWIKEAALAAGGTAQFAGGTPAMCDGVTQGQDGMDLSLFSRDVIAMATAVALSHQMFDGVLYLGVCDKIVPGLVMGALSFGHLPAVFVPGGPMTTGIGNEEKAHIRQLYAEGKIGRAELLEAETRSYHGPGTCTFYGTANSNQMLMEIMGLHLPGTAFINPGTPLREALTRHSATHALTLVHGGERYTPVADVLDERAFVNGIVGLVATGGSTNHTLHLIAMARVAGIQLTWDDFDELSAVVPLLARVYPNGKADVNQFQAAGGLALVIRGLLALGLLHDDVTTIAGKGLADYTREPVLRDGKLTWIDGPTASLDETIVRLPEQAFAPDGGIKLLDGNLGRCVIKTSAVKPEHQVVEAPAIVFERQDDLLDAFKRGELERDFVAVLPWQGPASCGMPELHKLTPTLTLLQDRGFHVALVTDGRMSGASGKVPAAIHVCPEALLGGAIGKVRTGDIVRVDAPAGVLTVKLADADLAARQADRPDLSRNRHGVGRDLFGGFRAHVSTAETGACSLFAEEEAERG
>NZ_CP043441.1|WP_149138538.1|1603811_1604756_-|mechanosensitive-ion-channel
MDLPYIDADKVQATWAYLTQFAVSQGLNCLAALAILIAGWWLSGRAARAVRQGLHHTHVDDTLRPMLASVAQWVVRVLTVVLVLSQFGVQTASIIAMLGAAGLAIGLALQGTLQNIAAGIMLVLLRPFRVGQYIDAQGVAGTVRETGLFMTELTTFDGVCLRVPNGKLWGSAIMNYSENVTRRMDIEIVVTFDSPVQAGLDALKAMLAKELRLLAEPRPEVMVTKYTDRGIALNARYWTSNDDFWNVQFGFFSKLKPTLEAAGCRIAVPVQEVLVPEMDDERDEATLADGAEGADGGGKADRYDARRTPPRRPS
>NZ_CP043441.1|WP_149138539.1|1605059_1606340_-|MFS-transporter
MSTATPVPAAEPDTVFRDPAFRHFWFARLCTTIGYQIFTVAVGWQMYDLTRDPFMLGMVGLVQFLPSIVLLLMSGHVADRFDRRLIVRTCQALEAVIAVSMAVASFSGWITREHIFLFVAAIGACRAFETPTLQALLPSVVTPRQLPRAVALASSAGQTAIIIGPALGGFAYVAGPGVVYSISAVLFAIAAVLVATLKLRQAAQRLTTPVSIKTLFAGFAYIRSHQVLLGAVSLDLFAVLLGGATALLPIYARDILHTGPWGLGLLRSSPAIGALVTALWLARNPLNRRVGRVMFGAVALFGVATMVFGISNWLPLSMGALVVLGASDMISVVVRQTLVQLDTPDDMRGRVGAVNSVFIGASNQLGEFESGVTAALLGPVGAVLLGGAGTVLVVFAWMRLFPALADRDRLHDHSHDHHHDRPAPAR
>NZ_CP043441.1|WP_149138540.1|1606541_1608680_+|TonB-dependent-siderophore-receptor
MLSAASRLTLAATGVALYTLSVPAVAQSSPPTLHAVTVIGNWLEAPDEEKVLEHPGARTIVDREVINETGANNVRDVLRRIPGVQVQENNGTGGSDLSLNVGVRGLTSRLSPRSTILMDGVPLAVAPYGQPQLSMAPLSLGNLSAVDVVRGAGSVRYGPQNVGGIINFVSRPIPQAFTAEAAIQTDVYSHGGNAKANPTAFLGGTADNGLGGALLYSGVHGNGYRAGNDYVDIDDMMLKGAYRISPADSLAATFHYFEGRGGLPGGLTQAQYAADPFQSTRPYDNFAGRRSDFSLRYAHDDRDRKVEVLTYYTDSFRGSEIENQRGAGSGVQRRLTTAPRNYHVFAIEPRYAQIFRTGSVGHEIGIGYRYLREASAEKATRTAFYAPGSVDAPGLPAPVYQSRTGGTTATAVYLDDTIHVGDWTITPGLRYEFIRTHLEDHVTGNRPALSSNEPLPSLAVMYRIRHNWTMFANAGVSYGPLQYFQLAQTTNGLVPEKAKTYEVGTHFDSLGGWGGELTLFNIDFDDALQLQGGTVGAPDAWTNLGATTHRGLESGLRYDFGTLSKSLAGLSAYATYTFTEATYKQGAFAGRDLPFYSRHVATLGLRYARNRWSVNVDGFGQSKQRSPGVPRTASAVYQTRESADGRYGDIPGYALMNLRVGYDFGKAAQNLKLAAGIKNVFDRRYYTRSTDNNFGKYVGMPRTFYVQASLAY

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Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity
NZ_CP043441_1	1.1\|1595170\|33\|NZ_CP043441\|CRISPRCasFinder	1595170-1595202	33	NZ_CP043441	Cupriavidus campinensis strain MJ1 plasmid unnamed1, complete sequence	1595170-1595202	0	1.0
NZ_CP043441_1	1.1\|1595170\|33\|NZ_CP043441\|CRISPRCasFinder	1595170-1595202	33	NZ_CP026305	Streptomyces lunaelactis strain MM109 plasmid pSLUN1, complete sequence	96887-96919	10	0.697
NZ_CP043441_1	1.1\|1595170\|33\|NZ_CP043441\|CRISPRCasFinder	1595170-1595202	33	NZ_CP026517	Deinococcus sp. NW-56 plasmid unnamed1, complete sequence	80170-80202	10	0.697
NZ_CP043441_1	1.1\|1595170\|33\|NZ_CP043441\|CRISPRCasFinder	1595170-1595202	33	NZ_MF600313	Mycolicibacterium sp. CBMA 213 plasmid pCBMA213_1, complete sequence	177334-177366	11	0.667

1. spacer 1.1|1595170|33|NZ_CP043441|CRISPRCasFinder matches to NZ_CP043441 (Cupriavidus campinensis strain MJ1 plasmid unnamed1, complete sequence) position: , mismatch: 0, identity: 1.0

gctttttccgctgcctgatgccgcccccgccgg	CRISPR spacer
gctttttccgctgcctgatgccgcccccgccgg	Protospacer
*********************************

2. spacer 1.1|1595170|33|NZ_CP043441|CRISPRCasFinder matches to NZ_CP026305 (Streptomyces lunaelactis strain MM109 plasmid pSLUN1, complete sequence) position: , mismatch: 10, identity: 0.697

gctttttccgctgcctgatgccgcccccgccgg	CRISPR spacer
agcgaccccgctccccgatgccgcccccgcctg	Protospacer
. .  ..***** **.*************** *

3. spacer 1.1|1595170|33|NZ_CP043441|CRISPRCasFinder matches to NZ_CP026517 (Deinococcus sp. NW-56 plasmid unnamed1, complete sequence) position: , mismatch: 10, identity: 0.697

gctttttccgctgcctgatgccgcccccgccgg	CRISPR spacer
agtgaccatgctgcctgatgccccccacgccgg	Protospacer
. *  .. .************* *** ******

4. spacer 1.1|1595170|33|NZ_CP043441|CRISPRCasFinder matches to NZ_MF600313 (Mycolicibacterium sp. CBMA 213 plasmid pCBMA213_1, complete sequence) position: , mismatch: 11, identity: 0.667

gctttttccgctgcctgatgccgcccccgccgg	CRISPR spacer
agcagctccgctggctgatgccgcacccgcgtc	Protospacer
. .  .******* ********** *****

Prophage detection

Region	Region Position	Protein_number	Hit_taxonomy	Key_proteins	Att_site	Prophage annotation

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

Overview of predicted results

Overview of the results

Cas Category Instructions

Results visualization

1. NZ_CP043439

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CRISPR-Cas detection and classification

Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection

2. NZ_CP043440

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CRISPR-Cas detection and classification

Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection

3. NZ_CP043441

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CRISPR-Cas detection and classification

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Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection