CRISPRimmunity

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Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Target MGE spacer number	Prophage number
NZ_CP031066	Bacillus sp. SH8-8 plasmid pl193, complete sequence	0 crisprs	csa3	0	0
NZ_CP031065	Bacillus sp. SH8-8 chromosome, complete genome	3 crisprs	cas3,csa3,WYL,DinG,cas14k,cas14j,DEDDh	3	7
NZ_CP031067	Bacillus sp. SH8-8 plasmid pl5, complete sequence	0 crisprs	NA	0	0

Results visualization

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CRISPR-Cas detection and classification

Crispr_ID: NZ_CP031065_1

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

NZ_CP031065_1

3142831-3143471

Orphan

Consensus_repeat	Method
CCTTGNATTCCTTGNGGACC	CRT

9 spacers

The CRISPR arrays of NZ_CP031065_1

>merge|NZ_CP031065|1|3142831-3143471|CRT
CCTTGTATGCCTTGTATTCCGGTCGGTCCTGTTGCACCAGTCACTCCAGTCGCTCCGGTTGCTCCGGTTGCTCCCGTCACACCTTGTGGCCCCTGGATTCCTTGGATTCCTTGTGGACCTGTTGCTCCCGTAGGGCCGGTCGCTCCCGCCTGCCCCGTTACCCCTTGTGGACCTTGGATTCCTTGTGGACCTTGAGGACCCGTTGCTCCAGTAGGTCCGGTTGCTCCTGTTGGACCAGTTTCTCCTTGTGGTCCTTGAATTCCTTGAGGACCTTGGGGACCCGTTGCACCAGTAGGTCCGGTTGCTCCTGTTGCTCCTGTTGGACCCGTCGAACCGGTTATCCCTTGAATTCCTTGCGGTCCAGTTGGGCCGGTTGGACCGATTACACCTTGAATTCCTTGTATACCTTGTATTCCCTGTGGACCCATTACTCCTTGTGGTCCTTGAATTCCCTGTATTCCTTGAGGACCAGTTGCACCAGTCGGACCGGTTGGGCCTTGTGGACCTTGCACACCTTGTATTCCTTGAGGACCGGTTGGGCCTATACTTCCTTGTGGTCCCTGAATACCTTGTATTCCTTGAGGACCAGTTGGACCGGTTATGCCACTCGGACCTTGTACGCCTTGCACTCCTTGAGGACC

>NZ_CP031065|1|1|3142831-3143471|CRT
CCTTGTATGCCTTGTATTCC	GGTCGGTCCTGTTGCACCAGTCACTCCAGTCGCTCCGGTTGCTCCGGTTGCTCCCGTCACACCTTGTGGCCCCTGGATT
CCTTGGATTCCTTGTGGACC	TGTTGCTCCCGTAGGGCCGGTCGCTCCCGCCTGCCCCGTTACCCCTTGTGGACCTTGGATT
CCTTGTGGACCTTGAGGACC	CGTTGCTCCAGTAGGTCCGGTTGCTCCTGTTGGACCAGTTTCTCCTTGTGGTCCTTGAATT
CCTTGAGGACCTTGGGGACC	CGTTGCACCAGTAGGTCCGGTTGCTCCTGTTGCTCCTGTTGGACCCGTCGAACCGGTTATC
CCTTGAATTCCTTGCGGTCC	AGTTGGGCCGGTTGGACCGATTACACCTTGAATTCCTTGTATA
CCTTGTATTCCCTGTGGACC	CATTACTCCTTGTGGTCCTTGAATT
CCCTGTATTCCTTGAGGACC	AGTTGCACCAGTCGGACCGGTTGGGCCTTGTGGACCTTGCACA
CCTTGTATTCCTTGAGGACC	GGTTGGGCCTATACTTCCTTGTGGTCCCTGAATA
CCTTGTATTCCTTGAGGACC	AGTTGGACCGGTTATGCCACTCGGACCTTGTACG
CCTTGCACTCCTTGAGGACC

Protein	Signature genes	Signature genes Name	Protein_function
NZ_CP031065.1\|WP_016122880.1\|3138073_3139072_-\|tryptophan--tRNA-ligase	unknown	unknown	gnl\|CDD\|183592
NZ_CP031065.1\|WP_000823781.1\|3151403_3151781_+\|hypothetical-protein	unknown	unknown	unknown
NZ_CP031065.1\|WP_000402258.1\|3153415_3154744_-\|HlyC/CorC-family-transporter	unknown	unknown	gnl\|CDD\|224173
NZ_CP031065.1\|WP_000539727.1\|3140896_3141145_-\|GlsB/YeaQ/YmgE-family-stress-response-membrane-protein	unknown	unknown	gnl\|CDD\|225170
NZ_CP031065.1\|WP_000613869.1\|3152949_3153363_-\|ATP-synthase-subunit-I	unknown	unknown	gnl\|CDD\|377159
NZ_CP031065.1\|WP_016122885.1\|3150190_3151105_-\|tetratricopeptide-repeat-protein	unknown	unknown	gnl\|CDD\|276809
NZ_CP031065.1\|WP_025708778.1\|3147502_3148258_-\|class-I-SAM-dependent-methyltransferase	unknown	unknown	gnl\|CDD\|379312
NZ_CP031065.1\|WP_149149818.1\|3148299_3148842_-\|CGNR-zinc-finger-domain-containing-protein	unknown	unknown	gnl\|CDD\|378696
NZ_CP031065.1\|WP_149149815.1\|3135024_3135864_-\|hypothetical-protein	unknown	unknown	unknown
NZ_CP031065.1\|WP_006922528.1\|3148954_3150136_+\|MFS-transporter	unknown	unknown	gnl\|CDD\|340887
NZ_CP031065.1\|WP_149149814.1\|3133915_3134935_-\|tyrosine-protein-phosphatase	unknown	unknown	gnl\|CDD\|379135
NZ_CP031065.1\|WP_000601226.1\|3141354_3141816_+\|GNAT-family-N-acetyltransferase	unknown	unknown	gnl\|CDD\|379326
NZ_CP031065.1\|WP_061113583.1\|3144847_3147355_-\|collagen-like-protein	unknown	unknown	gnl\|CDD\|367362
NZ_CP031065.1\|WP_149149819.1\|3151857_3152097_+\|DUF3923-family-protein	unknown	unknown	gnl\|CDD\|289806
NZ_CP031065.1\|WP_006922536.1\|3137618_3138065_+\|NUDIX-hydrolase	unknown	unknown	gnl\|CDD\|240033
NZ_CP031065.1\|WP_000938438.1\|3152202_3152751_-\|DUF2179-domain-containing-protein	unknown	unknown	gnl\|CDD\|235234
NZ_CP031065.1\|WP_149149816.1\|3135915_3136638_-\|GNAT-family-N-acetyltransferase	unknown	unknown	gnl\|CDD\|366181
NZ_CP031065.1\|WP_006922537.1\|3136761_3137601_+\|DUF2785-domain-containing-protein	unknown	unknown	gnl\|CDD\|371322
NZ_CP031065.1\|WP_000411484.1\|3139711_3140833_-\|AI-2E-family-transporter	unknown	unknown	gnl\|CDD\|223701
NZ_CP031065.1\|WP_000582026.1\|3132890_3133805_-\|phosphotransferase	unknown	unknown	gnl\|CDD\|225714

Protein	Function_ID	Function_description	E-value
NZ_CP031065.1\|WP_016122880.1\|3138073_3139072_-\|tryptophan--tRNA-ligase	gnl\|CDD\|183592	PRK12556, PRK12556, tryptophanyl-tRNA synthetase; Provisional.	0
NZ_CP031065.1\|WP_149149819.1\|3151857_3152097_+\|DUF3923-family-protein	gnl\|CDD\|289806	pfam13061, DUF3923, Protein of unknown function (DUF3923). This family of proteins is functionally uncharacterized. This family of proteins is found in bacteria. Proteins in this family are approximately 80 amino acids in length.	1.54071e-11
NZ_CP031065.1\|WP_000539727.1\|3140896_3141145_-\|GlsB/YeaQ/YmgE-family-stress-response-membrane-protein	gnl\|CDD\|225170	COG2261, COG2261, Predicted membrane protein [Function unknown].	4.0876e-09
NZ_CP031065.1\|WP_000613869.1\|3152949_3153363_-\|ATP-synthase-subunit-I	gnl\|CDD\|377159	pfam03899, ATP-synt_I, ATP synthase I chain. The atp operon of alkaliphilic Bacillus pseudofirmus OF4, as in most prokaryotes, contains the eight structural genes for the F-ATPase (ATP synthase), which are preceded by an atpI gene that encodes a membrane protein with 2 TMSs. A tenth gene, atpZ, has been found in this operon, which is upstream of and overlapping with atpI. AtpI is a Ca2+/Mg2+ transporter.	1.24519e-07
NZ_CP031065.1\|WP_016122885.1\|3150190_3151105_-\|tetratricopeptide-repeat-protein	gnl\|CDD\|276809	sd00006, TPR, Tetratricopeptide repeat. The Tetratricopeptide repeat (TPR) typically contains 34 amino acids and is found in a variety of organisms including bacteria, cyanobacteria, yeast, fungi, plants, and humans. It is present in a variety of proteins including those involved in chaperone, cell-cycle, transcription, and protein transport complexes. The number of TPR motifs varies among proteins. Those containing 5-6 tandem repeats generate a right-handed helical structure with an amphipathic channel that is thought to accommodate an alpha-helix of a target protein. It has been proposed that TPR proteins preferentially interact with WD-40 repeat proteins, but in many instances several TPR-proteins seem to aggregate to multi-protein complexes.	3.97455e-14
NZ_CP031065.1\|WP_000411484.1\|3139711_3140833_-\|AI-2E-family-transporter	gnl\|CDD\|223701	COG0628, yhhT, Predicted permease, member of the PurR regulon [General function prediction only].	2.99865e-58
NZ_CP031065.1\|WP_000402258.1\|3153415_3154744_-\|HlyC/CorC-family-transporter	gnl\|CDD\|224173	COG1253, TlyC, Hemolysins and related proteins containing CBS domains [General function prediction only].	1.44362e-160
NZ_CP031065.1\|WP_149149818.1\|3148299_3148842_-\|CGNR-zinc-finger-domain-containing-protein	gnl\|CDD\|378696	pfam11706, zf-CGNR, CGNR zinc finger. This family consists of a C-terminal zinc finger domain. It seems likely to be DNA-binding given the conservation of many positively charged residues. The domain is named after a highly conserved motif found in many members of the family.	5.26368e-18
NZ_CP031065.1\|WP_006922528.1\|3148954_3150136_+\|MFS-transporter	gnl\|CDD\|340887	cd17329, MFS_MdtH_MDR_like, Multidrug resistance protein MdtH and similar multidrug resistance (MDR) transporters of the Major Facilitator Superfamily. This family is composed of Escherichia coli MdtH and similar multidrug resistance (MDR) transporters from bacteria and archaea, many of which remain uncharacterized. MDR transporters are drug/H+ antiporters (DHA) that mediate the efflux of a variety of drugs and toxic compounds, and confer resistance to these compounds. MdtH confers resistance to norfloxacin and enoxacin. MdtH-like MDR transporters belong to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.	7.23116e-69
NZ_CP031065.1\|WP_149149814.1\|3133915_3134935_-\|tyrosine-protein-phosphatase	gnl\|CDD\|379135	pfam13350, Y_phosphatase3, Tyrosine phosphatase family. This family is closely related to the pfam00102 and pfam00782 families.	2.72341e-87
NZ_CP031065.1\|WP_000601226.1\|3141354_3141816_+\|GNAT-family-N-acetyltransferase	gnl\|CDD\|379326	pfam13673, Acetyltransf_10, Acetyltransferase (GNAT) domain. This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.	1.17689e-12
NZ_CP031065.1\|WP_061113583.1\|3144847_3147355_-\|collagen-like-protein	gnl\|CDD\|367362	pfam03157, Glutenin_hmw, High molecular weight glutenin subunit. Members of this family include high molecular weight subunits of glutenin. This group of gluten proteins is thought to be largely responsible for the elastic properties of gluten, and hence, doughs. Indeed, glutenin high molecular weight subunits are classified as elastomeric proteins, because the glutenin network can withstand significant deformations without breaking, and return to the original conformation when the stress is removed. Elastomeric proteins differ considerably in amino acid sequence, but they are all polymers whose subunits consist of elastomeric domains, composed of repeated motifs, and non-elastic domains that mediate cross-linking between the subunits. The elastomeric domain motifs are all rich in glycine residues in addition to other hydrophobic residues. High molecular weight glutenin subunits have an extensive central elastomeric domain, flanked by two terminal non-elastic domains that form disulphide cross-links. The central elastomeric domain is characterized by the following three repeated motifs: PGQGQQ, GYYPTS[P/L]QQ, GQQ. It possesses overlapping beta-turns within and between the repeated motifs, and assumes a regular helical secondary structure with a diameter of approx. 1.9 nm and a pitch of approx. 1.5 nm.	7.88052e-08
NZ_CP031065.1\|WP_006922536.1\|3137618_3138065_+\|NUDIX-hydrolase	gnl\|CDD\|240033	cd04677, Nudix_Hydrolase_18, Members of the Nudix hydrolase superfamily catalyze the hydrolysis of NUcleoside DIphosphates linked to other moieties, X. Enzymes belonging to this superfamily require a divalent cation, such as Mg2+ or Mn2+, for their activity and contain a highly conserved 23-residue nudix motif (GX5EX7REUXEEXGU, where U = I, L or V), which functions as a metal binding and catalytic site. Substrates of nudix hydrolases include intact and oxidatively damaged nucleoside triphosphates, dinucleoside polyphosphates, nucleotide-sugars and dinucleotide enzymes. These substrates are metabolites or cell signaling molecules that require regulation during different stages of the cell cycle or during periods of stress. In general, the role of the nudix hydrolase is to sanitize the nucleotide pools and to maintain cell viability, thereby serving as surveillance & "house-cleaning" enzymes. Substrate specificity is used to define families within the superfamily. Differences in substrate specificity are determined by the N-terminal extension or by residues in variable loop regions. Mechanistically, substrate hydrolysis occurs by a nucleophilic substitution reaction, with variation in the numbers and roles of divalent cations required.	3.47915e-59
NZ_CP031065.1\|WP_000938438.1\|3152202_3152751_-\|DUF2179-domain-containing-protein	gnl\|CDD\|235234	PRK04164, PRK04164, hypothetical protein; Provisional.	6.88941e-83
NZ_CP031065.1\|WP_149149816.1\|3135915_3136638_-\|GNAT-family-N-acetyltransferase	gnl\|CDD\|366181	pfam00583, Acetyltransf_1, Acetyltransferase (GNAT) family. This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.	2.29145e-06
NZ_CP031065.1\|WP_006922537.1\|3136761_3137601_+\|DUF2785-domain-containing-protein	gnl\|CDD\|371322	pfam10978, DUF2785, Protein of unknown function (DUF2785). Some members in this family are annotated as hypothetical membrane spanning proteins however this cannot be confirmed. The family has no known function.	1.31588e-39
NZ_CP031065.1\|WP_025708778.1\|3147502_3148258_-\|class-I-SAM-dependent-methyltransferase	gnl\|CDD\|379312	pfam13649, Methyltransf_25, Methyltransferase domain. This family appears to be a methyltransferase domain.	2.93212e-23
NZ_CP031065.1\|WP_000582026.1\|3132890_3133805_-\|phosphotransferase	gnl\|CDD\|225714	COG3173, COG3173, Predicted aminoglycoside phosphotransferase [General function prediction only].	6.21634e-07

>NZ_CP031065.1|WP_000601226.1|3141354_3141816_+|GNAT-family-N-acetyltransferase
MILKLNSTSFEVATSILQVQIPAYKVEADYLNSTAIPRLYDTVNDIQNCVETFYGYFSENKLVGFISFVQEEKLIDIHRLVVSPDFFQKGIATKLLIYIFNMFPSPMTYIVQTGKANTPAITLYKKHGFITVSDTILPDGMILTQLKKTEYTK
>NZ_CP031065.1|WP_000539727.1|3140896_3141145_-|GlsB/YeaQ/YmgE-family-stress-response-membrane-protein
MGWIITLIVGAIIGAIASSITGKNFPGGMFGNIIAGLLGASLGGKLFGTFGPSFGGIHVVPALLGAIVLIFIVSFVVKAAKK
>NZ_CP031065.1|WP_000411484.1|3139711_3140833_-|AI-2E-family-transporter
MEKINIRKRDKLRKFFREQNYLAVLLGFALLFINILLLTKISFVFTPFIVFLKTIFFPVLLAGVLFYILHPFVSLLEKKGVSRIVSIASIYLIVLGLFVFLVVTVIPIIKDQIDALIDNLPYFGHEIEQAARRFGESNLLGKIQENLNINVANMVKEYTVDFTKSLSSVTGNVTGFLSTVTEVVLTFVMVPFILFYLLKDGEQLPNHFLKFISEQRQPAAMRILDDMHYAISSYIRGQIIVSLFIGIMLLIGYLIIGIKYAVLLAILAMIVNIVPYVGPIIAITPALIIAFIDSPAMVLKVIIVMMVVQLAEGKFISPQVMGKKLDIHPITIIFIILTAGNLFGIMGIILAIPGYAILKVLVTHGYRFVKLNT
>NZ_CP031065.1|WP_016122880.1|3138073_3139072_-|tryptophan--tRNA-ligase
MSEKIMLTGIKPTGYPHLGNYIGAIKPALQMSKNNEGKALYFIADYHALNAVHDSEKFSSYTKEVAATWLSLGLGEDVIFYRQTEVPEILELAWILACLTPKGLMNRAHAYKAKVEQNKEAGLEVDAGVNMGLYTYPILMAADILLFQATHVPVGKDQIQHIEIARDIATYFNHTFGTTFTLPEYVIQEEGAILPGLDGRKMSKSYGNVIPLFAEQEKLRKLIFKIKTDSSLPNEPKELETLFTIYKEFATEDEIQSLREKYETGIGWGDVKKELFRVVDRELAGPREKYAMYMNEPDLLYEALENGAEKARAIAKVNLAEIKKRIGFKRER
>NZ_CP031065.1|WP_006922536.1|3137618_3138065_+|NUDIX-hydrolase
MGYIEDMRNLVGIHPLILIGSHAIILNKKDEVLLQLRTDFNRWGIIGGALEYNETLEDALKREVFEETGLIIKNPELFRTYSGPDYFQIYPNGDQVHGVLVVYICREFHGELVCDQSESKELRFFSLDELPNNLPPVIERIITDYQKK
>NZ_CP031065.1|WP_006922537.1|3136761_3137601_+|DUF2785-domain-containing-protein
MDITALQQQLELIQQNDYTQLQHIDVNELTLNMLQYIGTTDSYVRYQLIYKCFAHFIHHEFLMDDQLKLLLQTCLSDEYLYCDIYSPHTDGVFTRSYTVSLIALILQFANSHYFCTEEDIEEIKNKLITYTNLETDFRGYIENKGWAHCLAHVSDAFTEIVHNTYTTFEWYEELIHCLLNKIFIPSDLFHNNEDERIVTPLLAMLYHDFPQNDLISIIQKKIKRLPQIRKRLSLNEYCILCANIKTFLRTLFFRTKDDHNLAFTARKTERMLKELPNYY
>NZ_CP031065.1|WP_149149816.1|3135915_3136638_-|GNAT-family-N-acetyltransferase
MTTIEQLEKHFKIRRNASSIMIKENNAEIFTEEQLEEMMQYCVAEAKKDGLEKLHFEISSKSPNYDVYKRCFETYLFEYVTENMIVFKDIYEVEDVESEIDFKLIEEVGEEAFYSLWNEATGEPVAHDQFVHMMLQEIGEQWKEHCLTASADEEPIGIVIPHIERGTLEEGKLMYFAVTPNMRNKGYEAALFAGAMFVLKEIGASYYIGETNVQNEWMKDVFEKNGCQLLSSTERYVRKF
>NZ_CP031065.1|WP_149149815.1|3135024_3135864_-|hypothetical-protein
MLNQSMHSFVLLYSNVLLEGWNVEKVSERYEGEKWGTFWFQVVTPTGMFRVKEFFLDIMEPVFPDSCISQAKGDCFQYKGLVYWKGINYKGKESYVTSIWKTQVEISVDHGYVNQDEMERFLFELQPVNMELGKTILHTSFHLLSFQAKRSEMGEIGRCGEWNAPDDVSYVNLLIHEKLNWKLESVGFGNDETQYVYWDEVNKLYALWICRHKNKAYYPISSWTMNYGPKQIINGLEFYSHPNRGTVVYEDLGNEQIAYVFRGNLCTNFEQVKELFACL
>NZ_CP031065.1|WP_149149814.1|3133915_3134935_-|tyrosine-protein-phosphatase
MEQQRNWLQATVERIEDNTLQIKWENNIEEVRIYWSTSPDHIEENGELLATVNGELSYTIENPSENERPYFRLVGSNGQAVTVAERRLPLQGAFNFRDMGGYETTDGRKVKWGKLYRSEELAGLTEWDIDYLQKSGLKLICDYRTDFEVKHKPNPEITGARQVCLPVMQDLAKDLNINEFFQVGDLSMLGKPGEYLVKMNQDFVSGNEAFVSFLNLAQNPENLPLVNHCTAGKDRTGFGSALLLLLLGVPEKTVMEDYLLSNGFREKLNEKMMAFLGAKLQNDESRAILGAMFEARAEYLQAAIGEIQKQYGSVEAYAERALGFTKESLEEMQELLLED
>NZ_CP031065.1|WP_000582026.1|3132890_3133805_-|phosphotransferase
MIHTRVKAEGLREKLATILSQEYKELAIQDLKVIGTGVQNIVFQGDSEKGSLAFRVPWEREVENINEDLFNSRISLQKEAELSKYCHSKGIPVPRIHGLHLSTELDFLISDCVYADHMPISACKIGELVSNLHSMPIDGLYYKQNIREPISKYIAERIVKRIEGFNTITNCKINLPDTKTIEYILSTADHVKCLLHMDIRPANLIGYNGEVKAIVDWDNALIGHPLLELMRIAETNEVSWDEFKDGYKNDSIFNVTPHIVSLFYRLDTAVMLANLFIAHLKIKDKGVFYKERVKALHHDIYKNL
>NZ_CP031065.1|WP_061113583.1|3144847_3147355_-|collagen-like-protein
MRHHRKCKKFGVALPLPFIGATGPTGNSGPIGPTGGHEGPVGPTGVTGPTGATGPQGPQGIRGIQGPQGTTGAQGIQGAIGDTGEQGITGPTGLQGAQGLIGNQGLIGDIGVQGLEGIQGATGPAGSQGIQGTQGIQGEIGERGQTGAQGMQGERGITGVSGVTGPQGPQGVQGIQGEQGAIGIQGEDGFQGIQGITGEEGPQGAQGIQGEVGPTGSTGMQGAQGISGIKGITGAIGLQGPQGVQGIQGITGATGFQGIKGIRGITGATGSIGIQGSEGPPGGPTGTTGPIGPSSGVTGPAGPPGPPGGPTGPTGATGSTGVSGGIGPIGAQGVQGITGPTGPQGVRGAQGSQGVVGAVGVQGAQGPQGNQGITGPTGAEGSRGIQGPLGVIGPTGAEGPKGIQGIQGVIGPTGAQGMVGIQGVAGPAGVTGAEGAQGAQGIRGVTGPAGSQGIQGVQGIQGETGAAGIQGPQGVQGIQGTTGLTGTQGAQGPQGVQGIIGPTGAIGLQGSQGLQGITGPTGAQGVQGIQGVQGIIGPTGAQGIRGPQGSAGEVGITGPQGVQGAQGSQGPQGPQGNVGITGATGETGATGATGATGPQGVQGVQGITGIQGITGTTGNTGATGPQGIQGIQGIQGLQGPIGASGVTGASGSIGPVGAQGSQGPSGVVGPTGATGSAGSIGFLGIAGATGATGLPSGGGYFFSTSTSTIAANALIPINSGSTVFGSDVSLTNATTVTLSTPGIYLVSYYFQGDPTGGNETISVRLTLNGTQVAGSFIFYVTGGTFILEPAVSNTMVIEVTSSNATLSLQNGPLAIGHVTTLSGVITASLNVLKIV
>NZ_CP031065.1|WP_025708778.1|3147502_3148258_-|class-I-SAM-dependent-methyltransferase
MKGTQMLALNKKCWDTVAPYFFQVDCLPKYGPYTASEDEIRLFDSIRKKKVLDIGCGSGHSLQYMAEQGAEELWGLDLSSTQIEIANKTLKEWNPKLVCGAMEEEGDIPKGYFDIVYSIYALGWTLDLRKTLELIYSYVKPGGSFIFSWEHPVYSNLQYGTEEVAIKSSYHKETPITFETFKGENVQATLYKRKISTYINELNRAGFKIERIEEPEPSSSFDGELAEPSTKYYSLYKARMVPTTFIIKARK
>NZ_CP031065.1|WP_149149818.1|3148299_3148842_-|CGNR-zinc-finger-domain-containing-protein
MNQNAPNELEHIREFLNTWRIPNDTREPIDLLQTEEDIKLFMKEYFDEEFPFHTIEELRGFREDIRMTIEGEGSLQKWLEKYPFYVHIKDDMKDITYEPVYEENVYTKVLSIVLMSIQENVWGRLKACPDCRWVFYDHSRNGSKRWCGMYAGEEGGRACGTIAKVKNYRAKRKGRSGYDM
>NZ_CP031065.1|WP_006922528.1|3148954_3150136_+|MFS-transporter
MKSFSTPIRFMLISSFFMSFGYFAVYAFLAIYLLTFLHFSAVQVGTVLTVMTITSRVIPLFSGLIADKIGYIIMMISGLFLRGIGFIALGICSDFYTISISSALIGFGTAFYEPAARAIFGSQPAHLRKNLFTYLNLSFNCGAIIGPIAGGFLLLLDPLYAFSLTGSLMLLFAFIFYLLKNHFQVTTENTSITLGIQAILQNKSFLLFSFIMIFFYIMFTQLTVALPLHMKNISSSNQLATLVITINAITGVLFMVLFRKLFLKYNTLSFIKYGVLLMSISFLLIPLFQHPYWLFICVILFTIGETLVLPNADIAIANYSNESYTATFFGFYQLSLAFGFIIGNYTGTSFTSNLSGVYTPWLIFGGIGLTGFISLHILNIKKVASKEDRYIPM
>NZ_CP031065.1|WP_016122885.1|3150190_3151105_-|tetratricopeptide-repeat-protein
MNINEKAIEMFEQNKYEEAMELFQQAVHESRDVQSLNNLAWMYFYEEENDDKALELIREVVKLNPSSYFPYNILGDIYMKQEKWTEAKEALQKSISIQPSDEAYHNVAVAHYNLGELEKASEFFLRVAGDSDYIMYSYVKCLIDLERTTEAKEKLDTFNRESDNFLGEIHVADLYVELNCYKEAIEWFEKGYKKCWKSPNWISRFVYALYKVNHFSRINEVIRESIEAKTAEIKDVENEEVEDNWTEKDKKELIEEYTEANNDYKTMIERIKSGYVPGLEFETDFIGGCYLFGCKRHNHLEYEE
>NZ_CP031065.1|WP_000823781.1|3151403_3151781_+|hypothetical-protein
MKRKLLVLLFTGIILSGCSTIAENSLESGKGSMELLTPTITAKDNELEIKTEGIDENKVTFIYVANKKVLEQKLKNGESYKLNIKDIEHAHRTDYKPKVQLLQTKDDNDDGEIVTFKQVRYTVKN
>NZ_CP031065.1|WP_149149819.1|3151857_3152097_+|DUF3923-family-protein
MKKRWISWWIGNLFWIIVFGIWAAIIWLREIDGAGVIQTPEIKSISLIVILIAFIIPVFFQVIWLIINLRMSRKNNYTI
>NZ_CP031065.1|WP_000938438.1|3152202_3152751_-|DUF2179-domain-containing-protein
MLQALLIFVLQIIYVPILTIRTILLVKNQTRSAAGVGLLEGAIYIVSLGIVFQDLSNWMNIVAYVIGFSAGLLLGGYIENKLAIGYITYQVSLLDRCNELVDELRHSGFGVTVFEGEGINSIRYRLDIVAKRSREKELLEIINEIAPKAFMSSYEIRSFKGGYLTKAMKKRALMKKKDEHAS
>NZ_CP031065.1|WP_000613869.1|3152949_3153363_-|ATP-synthase-subunit-I
MIQEALRVYRLQLYVIFSGLLLMWTITPFGKQVTGFGIGLAVSAYCLWLLARRVEKLGKSIVMKEKAPGLGVLNRFAAAILGAIIMYEIEHEMEMWAFGTGILGGHFLMIANLAHANMQLVKEEEKQREHASKNIEL
>NZ_CP031065.1|WP_000402258.1|3153415_3154744_-|HlyC/CorC-family-transporter
MEIFNLVMVAILIAFTGFFVAAEFAIVKIRSSRIDQLVAEGKRGALAAKKVTTNLDEYLSACQLGITVTAMGLGWLGEPTIEKLLHPLFEKWNLNPSISSVLTFGLAFMIMTYLHVVVGELAPKTMAIQKAERVTLLLAGPLMMFYKVMYPFIWVLNGSARVITGLFGLKPASEHEVAHTEEELRLILSDSYESGEINQAEYKYVNNIFEFDNRIAKEIMVPRTEIVGFYLEDSVEEHMKVIQNERYTRYPIFGEDKDDIIGMVNVKDFFIRYMTEDQKDLSSIRSYMRPIIEVMETTPIHDLLLQMQKKRIPMAVLYDEYGGTAGIVTLEDILEEIVGEIRDEYDEDEAPPIQHVNEQHIIVDGKVLISEVKDLFGLHIEEDDVDTIGGWIMMQNHEIEEGQHVEAEGYEFKVLEKDAYQIKRVEIRKMEQEQEEEKAATV

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Crispr_ID: NZ_CP031065_2

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

NZ_CP031065_2

4606199-4606306

Orphan

Consensus_repeat	Method
TATATCTATCATAACTTACAATATAT	CRISPRCasFinder

1 spacers

The CRISPR arrays of NZ_CP031065_2

>merge|NZ_CP031065|2|4606199-4606306|CRISPRCasFinder
TATATCTATCATAACTTACAATATATCAACGATTTTTCAATTATATCTATCATAACTTATAATATATTAACGATTTTTCAATTATATCTATCATAACTTATAATATAT

>NZ_CP031065|2|1|4606199-4606306|CRISPRCasFinder
TATATCTATCATAACTTACAATATAT	CAACGATTTTTCAATTATATCTATCATAACTTATAATATATTAACGATTTTTCAAT
TATATCTATCATAACTTATAATATAT

Protein	Signature genes	Signature genes Name	Protein_function
NZ_CP031065.1\|WP_086389581.1\|4601329_4602055_-\|GntR-family-transcriptional-regulator	unknown	unknown	gnl\|CDD\|225099
NZ_CP031065.1\|WP_149149983.1\|4603820_4604924_-\|DgaE-family-pyridoxal-phosphate-dependent-ammonia-lyase	unknown	unknown	gnl\|CDD\|273623
NZ_CP031065.1\|WP_006915665.1\|4607344_4608451_-\|HAMP-domain-containing-histidine-kinase	unknown	unknown	gnl\|CDD\|223715
NZ_CP031065.1\|WP_149149986.1\|4613188_4614001_-\|2-succinyl-6-hydroxy-2,-4-cyclohexadiene-1-carboxylate-synthase	unknown	unknown	gnl\|CDD\|274729
NZ_CP031065.1\|WP_149149985.1\|4606401_4607262_-\|M15-family-metallopeptidase	unknown	unknown	gnl\|CDD\|350624
NZ_CP031065.1\|WP_149149984.1\|4604901_4606002_-\|amidohydrolase/deacetylase-family-metallohydrolase	unknown	unknown	gnl\|CDD\|132622
NZ_CP031065.1\|WP_149149978.1\|4596031_4598008_+\|FtsX-like-permease-family-protein	unknown	unknown	gnl\|CDD\|376899
NZ_CP031065.1\|WP_025709982.1\|4609423_4610530_-\|o-succinylbenzoate-synthase	unknown	unknown	gnl\|CDD\|239433
NZ_CP031065.1\|WP_149149982.1\|4603063_4603819_-\|oxo-acid-lyase	unknown	unknown	gnl\|CDD\|188342
NZ_CP031065.1\|WP_149149980.1\|4599955_4601281_-\|gluconate-permease-GntP	unknown	unknown	gnl\|CDD\|308194
NZ_CP031065.1\|WP_001290392.1\|4598061_4598253_-\|YjcZ-family-sporulation-protein	unknown	unknown	gnl\|CDD\|312989
NZ_CP031065.1\|WP_000132248.1\|4598509_4599103_+\|TetR-family-transcriptional-regulator	unknown	unknown	gnl\|CDD\|132427
NZ_CP031065.1\|WP_149149987.1\|4613997_4615752_-\|2-succinyl-5-enolpyruvyl-6-hydroxy-3--cyclohexene-1-carboxylic-acid-synthase	unknown	unknown	gnl\|CDD\|236020
NZ_CP031065.1\|WP_000425769.1\|4617337_4618291_+\|1,4-dihydroxy-2-naphthoate-polyprenyltransferase	unknown	unknown	gnl\|CDD\|235695
NZ_CP031065.1\|WP_016123513.1\|4615748_4617143_-\|isochorismate-synthase	unknown	unknown	gnl\|CDD\|224091
NZ_CP031065.1\|WP_000822509.1\|4608443_4609142_-\|response-regulator-transcription-factor	unknown	unknown	gnl\|CDD\|223816
NZ_CP031065.1\|WP_149149981.1\|4602054_4603071_-\|sugar-kinase	unknown	unknown	gnl\|CDD\|238571
NZ_CP031065.1\|WP_000449568.1\|4610530_4611976_-\|o-succinylbenzoate--CoA-ligase	unknown	unknown	gnl\|CDD\|235146
NZ_CP031065.1\|WP_000963885.1\|4612300_4613119_-\|1,4-dihydroxy-2-naphthoyl-CoA-synthase	unknown	unknown	gnl\|CDD\|180958
NZ_CP031065.1\|WP_149149979.1\|4599148_4599820_+\|hypothetical-protein	unknown	unknown	unknown

Protein	Function_ID	Function_description	E-value
NZ_CP031065.1\|WP_086389581.1\|4601329_4602055_-\|GntR-family-transcriptional-regulator	gnl\|CDD\|225099	COG2188, PhnF, Transcriptional regulators [Transcription].	2.951e-45
NZ_CP031065.1\|WP_149149983.1\|4603820_4604924_-\|DgaE-family-pyridoxal-phosphate-dependent-ammonia-lyase	gnl\|CDD\|273623	TIGR01437, selA_rel, uncharacterized pyridoxal phosphate-dependent enzyme. This model describes a protein related to a number of pyridoxal phosphate-dependent enzymes, and in particular to selenocysteine synthase (SelA), which converts Ser to selenocysteine on its tRNA. While resembling SelA, this protein is found only in species that have a better candidate SelA or else lack the other genes (selB, selC, and selD) required for selenocysteine incorporation. [Unknown function, Enzymes of unknown specificity].	0
NZ_CP031065.1\|WP_006915665.1\|4607344_4608451_-\|HAMP-domain-containing-histidine-kinase	gnl\|CDD\|223715	COG0642, BaeS, Signal transduction histidine kinase [Signal transduction mechanisms].	9.71851e-52
NZ_CP031065.1\|WP_149149986.1\|4613188_4614001_-\|2-succinyl-6-hydroxy-2,-4-cyclohexadiene-1-carboxylate-synthase	gnl\|CDD\|274729	TIGR03695, menH_SHCHC, 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase. This protein catalyzes the formation of SHCHC, or (1 R,6 R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate, by elmination of pyruvate from 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC). Note that SHCHC synthase activity previously was attributed to MenD, which in fact is SEPHCHC synthase. [Biosynthesis of cofactors, prosthetic groups, and carriers, Menaquinone and ubiquinone].	6.97186e-129
NZ_CP031065.1\|WP_149149985.1\|4606401_4607262_-\|M15-family-metallopeptidase	gnl\|CDD\|350624	cd14852, LD-carboxypeptidase, L,D-carboxypeptidase DacB and LdcB, and related proteins. This L,D-carboxypeptidase family includes LdcB LD-Carboxypeptidase from Streptococcus pneumoniae, Bacillus anthracis, and Bacillus subtilis, and L,D-carboxypeptidase DacB from Streptococcus pneumonia and Lactococcus lactis. These enzymes are active against cell-wall-derived tetrapeptides and synthetic tetrapeptides lacking the sugar moiety but are inactive against tetrapeptides terminating in L-alanine. L,D-carboxypeptidase DacB plays a key role in the remodeling of S. pneumoniae peptidoglycan during cell division. It adopts a zinc-dependent carboxypeptidase fold and acts as an L,D-carboxypeptidase towards the tetrapeptide L-Ala-D-iGln-L-Lys-D-Ala of the peptidoglycan stem. This family also includes vanY D-Ala-D-Ala carboxypeptidase which is vancomycin-inducible and penicillin-resistant. VanY hydrolyzes depsipeptide- and D-alanyl-D-alanine-containing peptidoglycan precursors; it is insensitive to beta-lactams. All these enzymes belong to the MEROPS family M15 subfamily B.	1.77281e-52
NZ_CP031065.1\|WP_149149984.1\|4604901_4606002_-\|amidohydrolase/deacetylase-family-metallohydrolase	gnl\|CDD\|132622	TIGR03583, EF_0837, probable amidohydrolase EF_0837/AHA_3915. Members of this family of relatively uncommon proteins are found in both Gram-positive (e.g. Enterococcus faecalis) and Gram-negative (e.g. Aeromonas hydrophila) bacteria, as part of a cluster of conserved proteins. These proteins resemble aminohydrolases (see pfam01979), including dihydroorotases. The function is unknown. [Hypothetical proteins, Conserved].	0
NZ_CP031065.1\|WP_149149978.1\|4596031_4598008_+\|FtsX-like-permease-family-protein	gnl\|CDD\|376899	pfam02687, FtsX, FtsX-like permease family. This is a family of predicted permeases and hypothetical transmembrane proteins. Buchnera aphidicola LolC has been shown to transport lipids targeted to the outer membrane across the inner membrane. Both LolC and Streptococcus cristatus TptD have been shown to require ATP. This region contains three transmembrane helices.	0.000180794
NZ_CP031065.1\|WP_025709982.1\|4609423_4610530_-\|o-succinylbenzoate-synthase	gnl\|CDD\|239433	cd03317, NAAAR, N-acylamino acid racemase (NAAAR), an octameric enzyme that catalyzes the racemization of N-acylamino acids. NAAARs act on a broad range of N-acylamino acids rather than amino acids. Enantiopure amino acids are of industrial interest as chiral building blocks for antibiotics, herbicides, and drugs. NAAAR is a member of the enolase superfamily, characterized by the presence of an enolate anion intermediate which is generated by abstraction of the alpha-proton of the carboxylate substrate by an active site residue and is stabilized by coordination to the essential Mg2+ ion.	0
NZ_CP031065.1\|WP_149149982.1\|4603063_4603819_-\|oxo-acid-lyase	gnl\|CDD\|188342	TIGR03581, EF_0839, conserved hypothetical protein EF_0839/AHA_3917. Members of this family of relatively uncommon proteins are found in both Gram-positive (e.g. Enterococcus faecalis) and Gram-negative (e.g. Aeromonas hydrophila) bacteria, as part of a cluster of conserved proteins. The function is unknown. [Hypothetical proteins, Conserved].	6.44804e-136
NZ_CP031065.1\|WP_149149980.1\|4599955_4601281_-\|gluconate-permease-GntP	gnl\|CDD\|308194	pfam02447, GntP_permease, GntP family permease. This is a family of integral membrane permeases that are involved in gluconate uptake. E. coli contains several members of this family including GntU, a low affinity transporter, and GntT, a high affinity transporter.	1.1874e-136
NZ_CP031065.1\|WP_001290392.1\|4598061_4598253_-\|YjcZ-family-sporulation-protein	gnl\|CDD\|312989	pfam09680, Tiny_TM_bacill, Protein of unknown function (Tiny_TM_bacill). This entry represents a family of hypothetical proteins, half of which are 40 residues or less in length. Members are found only in spore-forming species. A Gly-rich variable region is followed by a strongly conserved, highly hydrophobic region, predicted to form a transmembrane helix, ending with an invariant Gly. The consensus for this stretch is FALLVVFILLIIV.	0.000128851
NZ_CP031065.1\|WP_000132248.1\|4598509_4599103_+\|TetR-family-transcriptional-regulator	gnl\|CDD\|132427	TIGR03384, betaine_BetI, transcriptional repressor BetI. BetI is a DNA-binding transcriptional repressor of the bet (betaine) regulon. In sequence, it is related to TetR (pfam00440). Choline, through BetI, induces the expression of the betaine biosynthesis genes betA and betB by derepression. The choline porter gene betT is also part of this regulon in Escherichia coli. Note that a different transcriptional regulator, ArcA, controls the expression of bet regulon genes in response to oxygen, as BetA is an oxygen-dependent enzyme. [Regulatory functions, DNA interactions].	2.36254e-12
NZ_CP031065.1\|WP_149149987.1\|4613997_4615752_-\|2-succinyl-5-enolpyruvyl-6-hydroxy-3--cyclohexene-1-carboxylic-acid-synthase	gnl\|CDD\|236020	PRK07449, PRK07449, 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase; Validated.	0
NZ_CP031065.1\|WP_000425769.1\|4617337_4618291_+\|1,4-dihydroxy-2-naphthoate-polyprenyltransferase	gnl\|CDD\|235695	PRK06080, PRK06080, 1,4-dihydroxy-2-naphthoate octaprenyltransferase; Validated.	5.86425e-131
NZ_CP031065.1\|WP_016123513.1\|4615748_4617143_-\|isochorismate-synthase	gnl\|CDD\|224091	COG1169, MenF, Isochorismate synthase [Coenzyme metabolism / Secondary metabolites biosynthesis, transport, and catabolism].	3.47815e-117
NZ_CP031065.1\|WP_000822509.1\|4608443_4609142_-\|response-regulator-transcription-factor	gnl\|CDD\|223816	COG0745, OmpR, Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain [Signal transduction mechanisms / Transcription].	3.63607e-91
NZ_CP031065.1\|WP_149149981.1\|4602054_4603071_-\|sugar-kinase	gnl\|CDD\|238571	cd01166, KdgK, 2-keto-3-deoxygluconate kinase (KdgK) phosphorylates 2-keto-3-deoxygluconate (KDG) to form 2-keto-3-deoxy-6-phosphogluconate (KDGP). KDG is the common intermediate product, that allows organisms to channel D-glucuronate and/or D-galacturinate into the glycolysis and therefore use polymers, like pectin and xylan as carbon sources.	1.88566e-82
NZ_CP031065.1\|WP_000449568.1\|4610530_4611976_-\|o-succinylbenzoate--CoA-ligase	gnl\|CDD\|235146	PRK03640, PRK03640, o-succinylbenzoate--CoA ligase.	0
NZ_CP031065.1\|WP_000963885.1\|4612300_4613119_-\|1,4-dihydroxy-2-naphthoyl-CoA-synthase	gnl\|CDD\|180958	PRK07396, PRK07396, dihydroxynaphthoic acid synthetase; Validated.	0

>NZ_CP031065.1|WP_149149984.1|4604901_4606002_-|amidohydrolase/deacetylase-family-metallohydrolase
MTERFVLRNVKRVNGEEIDIVIENNKIAQVTKAGAGEGGKVLDYSGTYVSSGWIDLHVHAFPEFDPYGDEVDEIGVKQGVTTIVDAGSCGADRIADLVQSREQAKTNLFAFLNISRIGLKRIDELSNMEWIDKEKVIEAVEKYKDVIVGLKARMSKSVVCDSGIEPLHVARSLSRETSLPIMVHIGSAPPRIEEVVPLLEKDDVITHYLNGKENNLFDKEGKPLPVLLDAVNRGVHLDVGHGNASFSFKVAEAAKRHGIAFHTISTDIYRKNRIHGPVYSMAHVLSKFLYLGYPLEEVIDAVTKNAAEWLKKPELGRIQEGDIANLTLFTVKDEKVTLIDSEGDQRIAERRIDTKGVVINGSFIEC
>NZ_CP031065.1|WP_149149983.1|4603820_4604924_-|DgaE-family-pyridoxal-phosphate-dependent-ammonia-lyase
MGHSLNAKYGLKRVINASGRMSILGVSAPTDTVMDAMKHGGQNYVEIADLVDKAGDHIARILDSEAAVVVNSASSGIALSIAGIVTEGNRRKSERLHQEVIAKNEVIMLKGHNVQYGAPVETMIYLGGGKLVEVGYANEGKAEHIEDAIGENTAAILYVKSHHAVQKNMISVEEAWEVAQRNKVPLIVDAAAEEDIQKYVKYSDLAIYSGSKAIEGPTSGIVGGKRKYIEWLKVQLHCIGRSMKVGKETTFGLLQALDEYGVKEDKSEQEKELLQVLMPLKELNGVNVTIVQDEAGRAIFRARIHINEKELNKSAKDVVTALREGEIAIYTRDYGVRQGFFDIDPRPLQGDDIHVIEEKLREIVGGN
>NZ_CP031065.1|WP_149149982.1|4603063_4603819_-|oxo-acid-lyase
MTNIQKRFYKGRVALNVLANNIENAKDIFEAAEGYVVVGVLSKDYATVEEAVTAMKAYGKEIDDAVSIGLGAGDNRQAAVVAEIAKHYPGSHINQVFPSVGATRANLGEKDSWINSLVSPTGKVGYVNISTGPISAAGEEKAIVPIKTAIALVRDMGGNSLKYFPMKGLAHEEEYRAVAKACAEEGFALEPTGGIDKENFETIVRIALEANVEQVIPHVYSSIIDKETGNTKVEDVRELLAVVKKLVDQYA
>NZ_CP031065.1|WP_149149981.1|4602054_4603071_-|sugar-kinase
MRKKIAAFGEVMMRLQVPGYELLSQANTLNYSFSGTGVNVAAALSHLGHEGLLISTLPENSVGDAAVSYIQKLGIQTSLVSRGGKYVGMYFLENGFGARASRVTYSNRIESSFNTASEEMYQFEEIAKEIDIVHFCGITLAMNDTVRHHMKSLARAVKENGGTVVFDCNYRPSLWGEDGYEQAKPHYEEMLGLADIVMMNEKDAMFVLGIKTEETEREAQLVDLIPRVAETYHIATIAGTHRSINSDNTHSLRGFICKDGTFTFAKTLTFSVYDRIGAGDAYTSGIIHGEIKEFAPEKAVSFASAAGMLAHTIVGDTPMSSEKDILRAMTASVGDVER
>NZ_CP031065.1|WP_086389581.1|4601329_4602055_-|GntR-family-transcriptional-regulator
MGVTVNVTRKKGPLYLQIKNIIRDRILHGVYAIHTNIPSEPQLEEEFKVSKITVRNAIKELAQEGYLEKKSGKGTKVIRNTSATKLSKGKKFTEVLVEEGYKVQKKLLKAEIVQNEEGTVPFRLFGKESFRIERLYTLNDAPYIHYTHYFSAGMASSDLSDFDLQSLYDLVEDRGIHLENFRDEFAVGLATSFVAEALGEKEGTALLKRMRYSYDEVGEVIEYSEGYYNTEMQHYVVNYDV
>NZ_CP031065.1|WP_149149980.1|4599955_4601281_-|gluconate-permease-GntP
MDIYLLIVTLLAIAIVILGVSWWKWHAFISLTVASLFLAIMSGLNVTKIVTAYETGVGSVLGHLVGILALGTILGKMMSDSGAGMQVADFFIRFFGVKKLPWAMLFAGFVIGIPVFFEVGIVILLPLVISIRKTTKQNILLIALPVIAGLSIVHGLVPPHPGAMTAIGIYNANLGKVLLYSLIIALPTAIIAGPIFAKWVHKRVIPENEPELIRVTTVSTDLPSRKVSFFIILLPVVLMILSVVAPYISLSKKLTEFFVFIGSPVIALLISCFAAFYLLGIKQGINKKMIKKLTDESLLPVGSIILIIGAGGGFKQILIESGVGTAIAQMAEHISLSPIVLAFMVAGLIRIATGSATVALTTAAGIVSPVIQHMSGVNLELLVIATGAGSLMFSHVNDAGFWLVKEYLGLTVKETFKTWTVLETLLSFIAFGFALVLNMFL
>NZ_CP031065.1|WP_149149979.1|4599148_4599820_+|hypothetical-protein
MKKASVIGLGFLEKLLAIVSPILLGVIGYFSPKLIEIIKRIPLFSDSKIIELLNFINPFWLPIILMLIGVIAGILFSLIIYSEALKMNITSESIHVNKDDQKKQITRSNVKAVFVDKRQVVIIDQEDREWLRETSDITPSKIKDTFLMHGFPWVEQDPYKHEYSLWKLEDERFNKSINTILYERRNALREGNSKKKKHLRKDLNELGVFVKDDGEDQYVRSIN
>NZ_CP031065.1|WP_000132248.1|4598509_4599103_+|TetR-family-transcriptional-regulator
MTDSFIAEARREQIILACIDTLEEVGYNNLSLTKVAKKAKISTGLISYHFNDKLYLMNRTLQFLVAKQHEFISNRVLLAQSEINKLEAFIEAHLAYQETHYKNNIALIEIVFNARNEENVPYYRIEDDEEEDALRTMLLDILKTGQQNGVFSDSFQTDTLASFILGAIEERMLKANSSISIENYSDELIKMVKKLTV
>NZ_CP031065.1|WP_001290392.1|4598061_4598253_-|YjcZ-family-sporulation-protein
MSEKCEHRHDDCNRRHGCGGGFALLIVLFILLIIIGASCFGGGGGCGYGGYGGYAGGYGGYCC
>NZ_CP031065.1|WP_149149978.1|4596031_4598008_+|FtsX-like-permease-family-protein
MTFWQFAFKNVSRNSKAYFAYFVSSAFSIMVFFSFTVYAYHPRLQSVQSFQDRDPLMNLASTAQLVIVMFSFFFLLYSIGTFLNVRKQQFGILTILGISQRQLKRLLFTENMIIGILSIFIGIQGGLVFSNFFLLVTSKLTSAKGLYLYWPTEAIIVTTVTFIILFLIVSTFTPMFIRTRKTTKLIKGVNKEKSEKKPSILISLFALTCLGLCYYIAGYPQGYITEKNAQNGSVFLIMLSILPLVVIGTYLFFSQTFLLFIYILKKRRKFYLKQINMLWISDLVSRTRSNINVLFIVSMLSALAFTIIIGLFAANNNTKAPILERYPIPFTYTSEGVNAFEQKHITTIETELTNANFQYDKYKFTVLKDTASKESIAIMKMSDYNAIAQQLNRPEITLDSSQVYNISRYSPVLLNLVSNPFAKQNTITLGSNKKEFQIKGFINRGIEPPFALPHLIVMQDEVIENMIPHIETITVYNYFVENWENAIVPTKNILKSLNHDAETLYEAHKNEEDFIKPFYIYTATDELVHSKGNAVAQFFIWAFLGFIFFIGAASVLYFRMYNDLTTERQKYITITKLGLTESEMFRSATIQLGILFFVPYIVAGVHTLFAVKFLQSMFSFSLLKETCIVLTFFGIIEIIFFFLIRSLYINKLSQHIKI
>NZ_CP031065.1|WP_149149985.1|4606401_4607262_-|M15-family-metallopeptidase
MKKWVFISFFIACTVCVGVYISPLFQKEIDVKIVGKDELVKAANTEGREITKEQIYKGDLLLVNRDYPVKKDSIRSDIINVNHNSELVRGYVIFDRNLRLSKDVVKKFLNVVDAAGKESVNHFLISSGYRDFQEQKQLYEKMGSDYALPAGYSEHNLGLSLDIGSTQKKMEKAPEGKWIEENVWKHGFVLRYPKNKSNITGIQYEPWHIRYVGLPHSAIMQKKNFTLEEYLEFLKEEKEISTEVEGKKYTVSYYKVSENMKVNVPANKQYEISGNNMDGVIVTVQE
>NZ_CP031065.1|WP_006915665.1|4607344_4608451_-|HAMP-domain-containing-histidine-kinase
MANMMKSFRFKMIALFALSMVLAATVTYIIYKGLQLYYKTMVRYEEPLAQFRSMVREFGDINFFLIFFIPLSIIFFFFLTKPYLKYFDEISNGIHHLANGNFTNKVQVSSNDEFGNIAREINVASEKLKEAVERGDFAESSKSQLVVNLAHDLRTPLTSVLGYLDLILKDENLTKAQIKHFSTIAYTKSQRLESLIDELFEITRMNYGMLKLDKKPIDISELLIQLEEELYPLLEKHHLEARLNVAPHLPMHGDGKLLARVFENLLTNAVRYGYDGQFVDMNGYVDNGEVVVQIMNYGDSIPEEDLPYLFDMFYTGDKARTENRGGTGLGLFIAKNIVEQHNGTISAESNVVRTLFEVRLPKDENVTI
>NZ_CP031065.1|WP_000822509.1|4608443_4609142_-|response-regulator-transcription-factor
MKRISILIADDEAEIADLIEIHLEKEGYHVVKAADGEEAVHIIETQPIDLVILDIMMPKMDGYEVTRQIRTKHHMPIIFLSAKTSDFDKVTGLVLGADDYMTKPFTPIELVARVNAQLRRFFTLNQPKVAESKSALEIGGVVINPERRTVDVYGEQIELTPKEFDILYLLASHPKKVYNVENIFQHVWADDYYEGGNTVMVHIRTLRKKLGEDKRKDKLIKTVWGVGYTFNG
>NZ_CP031065.1|WP_025709982.1|4609423_4610530_-|o-succinylbenzoate-synthase
MEIKKATLYITEMPLVIPFAASYGTYEKRESIVIELEDTDGYIGFGEVVAFSEPWYTEETVKTALHILQDFLIPDLLKAEISHPNEVPGLFQHIKRNRMAKAGIEGAVWDLYAKRQKKSLATLLGGTKSEIEVGVVIGLNTIPVMLKQIEKYAEEGYERFKVKIKPGHDYELLKEIRKEFPHIPLMADANSAYTLGDTERLKRLDEFQLMMIEQPLADYDFLDHAELQKKIETPICLDESIHSLEDARVAITLGSCQIVNIKPGRVGGLTESVQIHNYCMEHNVPVWCGGMVEMGISRAQNVALASLPNFTIPGDISASSRHWEQDIIWPEVTLEGGKVMVPQSVDAEYEVDRGRLEEITKQRIVFER
>NZ_CP031065.1|WP_000449568.1|4610530_4611976_-|o-succinylbenzoate--CoA-ligase
METMPNWLMQRAFLTPDRTAIEIEEEKVTFMELHEKVVSVCEHLTHVGVERGQKVAVLMKNGMEMITVIHALSYVGAVAVLLNTRLSREELLWQMDDAEVVCLVTDQDFDAKDVPVYSFAEVMNGPKEEASIQEEFSLEEAMTIIYTSGTTGKPKGVILTYGNHWASAVGSSLNLGLRDDDCWLACMPMFHVGGLSLLMKNIMYGMRILLVPKYDADFIHKALQTRGVTIISVVSKMLTDLLERLGEGTYPASLRCMLLGGGPAPKPLLETCVEKGIPVYQTYGMTETSSQICTLSADYMLTKVGSAGKPLFQCQLRIEKDGVVVPPFAEGEIVVKGPNVTGGYFNREDATRETIQNGWLHTGDLGYLDEEGFLYVLDRRSDLIISGGENIYPAQIEEVLLSHPAVAEAGVVGMADDKWGQVPAAFVVKSGAVTEEEILHFCEEKLAKYKVPKKACFLEELPRNASKKLLRRELRQLVEEM
>NZ_CP031065.1|WP_000963885.1|4612300_4613119_-|1,4-dihydroxy-2-naphthoyl-CoA-synthase
MAIEWVKEGNYEDIIYSTYNGIAKISINRPEVHNAFRPKTVMELIDAFAQARDDANVGVIILTGEGGRAFCSGGDQKVRGHGGYVGDDQIPRLNVLDLQRLIRAIPKPVIAMVAGYAIGGGHVLHIVCDLTIAADNAVFGQTGPKVGSFDGGYGAGYLARMVGHKKAREIWYLCRQYSAQEALDMGLVNTVVPLEELEAETVQWAQEILANSPMALRFLKAAFNADTDGLAGIQQLAGDATLLYYTTDEAKEGRDAFKEKRSPDFGQFPRFP
>NZ_CP031065.1|WP_149149986.1|4613188_4614001_-|2-succinyl-6-hydroxy-2,-4-cyclohexadiene-1-carboxylate-synthase
MKVTLQGVSYEYEVVGSGEPLLLLHGFTGSMETWRSFISSWSEQFQVIVVDLVGHGKTESPEDVAHYDIRNVALQMKELLDYLHIEKVHILGYSMGGRLAITMACLYPDYVHSLLLENCTAGLEDEAHRKERREKDDRLADKIEREGIESFVSMWENIPLFATQKRLAKNVQEAVRKERLANNPKGLANSLRGMGTGAQPSWWDELKNLKIPVLLMNGEYDEKFFRILKNIEKCISDAKFVKIDGAGHAIHVEQPEKFDTIVKGFLKTMQ
>NZ_CP031065.1|WP_149149987.1|4613997_4615752_-|2-succinyl-5-enolpyruvyl-6-hydroxy-3--cyclohexene-1-carboxylic-acid-synthase
MNNHIEALSYYLGAFVDELTRLDVCDVVISPGSRSTPIALLMEQHEGMNTYLHVDERSAGFFALGIAKAKKRPVALLCTSGTAAANYYPAVCEAFHSRVPLIVLTADRPHELRDVGAPQAMNQFNLYGTFVKQFTEMALPEASEAMYHYARMTTQRIVANACLAPQGPVHLNFPVREPLIPDFSLESLWDKGRSEYTGVVQQGNAVMPSEYVDSLVGRLSHMEKGLIICGDDSHAEIAIFATQLAEKTGYPILADPLSNIRSGHHDKTMVIDCYDTFLRNEKLKETWKPDVLIRFGGMPVSKSLTQFIKKQTKAVHIVVDESGQWRDPALVATEVVQASDIEFCKALIEKMPVMKKNDWSLMWQHINEKTKETLREMETYETAFEGKVITDIVRVLPEDATLFASNSMPIRDTDSFFFTSDKNIHVMANRGVNGIDGIISTALGASVICDPLVLVIGDLSFYHDLNGLLAAKLHELNITIVVVNNDGGGIFSFLPQYEKKEHFESLFGTPIGLDYEHVVKMYGGSFSRVNGWEQFREEVRKGTTTEGLHVVEICTNRDENLTLHRTLWAKTMDVITTSLQGESK
>NZ_CP031065.1|WP_016123513.1|4615748_4617143_-|isochorismate-synthase
MIQTKQKGLQEVLIAAIKRATNEKTLVSFVKQIDWVDPLLFYAAGKRISFENRCYFADPAQHVIFAGIGSVFTIANSSHKRFQTARDEWEKVKENAFVQRDEYEFGTGPLLFGGFSFDPEKEKTDLWKEFKDTTFSLPAFLLTVKNEKAWLTMNTFVSATDCAENLYNEMISLEEKILRESKCALNGSKLTVISKVEVDPKGWMKAIEKVQDEMKQGNVQKVVLARELKVEMNHSIDSALVLEALRIGQPDCYVFSFDYKGACFLGATPERLIRKEDEKFTSMCLAGSIGHGHSIEESKRNSNALLHDEKNLAEHGYVVNMIRSVLNEHCEYVNIPESPGLLTTKNLIHLYTPVEAKGDASLLTMVEELHPTPALGGTPRLEAMKLIRDVELLDRGLYGAPIGWIDDEGNGEFAVALRCGLLNGEKASLFAGCGIVIDSIPQLEYEETSLKFRPMLGALEELMK
>NZ_CP031065.1|WP_000425769.1|4617337_4618291_+|1,4-dihydroxy-2-naphthoate-polyprenyltransferase
MEMNVKTNSSPTAPKPSKQTGWRIWWSLLRPHTLTAAFVPVFIGTAYSMQVGGINQIHLPLFLMMLLACLLIQAATNMFNEYFDYKRGLDHEGSVGIGGAIVRDGIQPKTVLNLAFGFFGIAILLGVYICMNSSWWLAAIGLVCMAVAYLYTGGPLPIAYTPFGELTAGLFMGVIIIGISFFIQTGTVTSEVILLSIPSSILIGAILLANNIRDLDGDKENGRKTLAILVGRERAVGVLASMFIVSYIWTIALIIVGIVSPWMLIVFLSAPKAFKATKGFIGKSIPIEMVPAMIATAKTNTIFGFLMGIGLLLGYFL

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Crispr_ID: NZ_CP031065_3

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

NZ_CP031065_3

5026052-5026185

Orphan

Consensus_repeat	Method
GTTGATTTCTCTTCTTTTTGAGA	CRISPRCasFinder

2 spacers

The CRISPR arrays of NZ_CP031065_3

>merge|NZ_CP031065|3|5026052-5026185|CRISPRCasFinder
GTTGATTTTTCTTCTTTCTTCGGTTCTTCTTTTTTGTCACTAGTTGAACCGCTTGTTGATTTCTCTTCTTTTTGAGACTTGTCTTCTGTTTTACTTTCAGGTTTCGTGCTCGTTGACTTCTCTTCTTTTTGAGA

>NZ_CP031065|3|2|5026052-5026185|CRISPRCasFinder
GTTGATTTTTCTTCTTTCTTCGG	TTCTTCTTTTTTGTCACTAGTTGAACCGCTT
GTTGATTTCTCTTCTTTTTGAGA	CTTGTCTTCTGTTTTACTTTCAGGTTTCGTGCTC
GTTGACTTCTCTTCTTTTTGAGA

Protein	Signature genes	Signature genes Name	Protein_function
NZ_CP031065.1\|WP_000672659.1\|5029906_5030926_-\|stage-II-sporulation-protein-D	unknown	unknown	gnl\|CDD\|274332
NZ_CP031065.1\|WP_149150074.1\|5022101_5022779_-\|polysaccharide-biosynthesis-tyrosine-autokinase	unknown	unknown	gnl\|CDD\|349772
NZ_CP031065.1\|WP_149150130.1\|5018401_5019316_-\|UTP--glucose-1-phosphate-uridylyltransferase-GalU	unknown	unknown	gnl\|CDD\|224131
NZ_CP031065.1\|WP_149150072.1\|5019528_5020296_-\|tyrosine-protein-phosphatase	unknown	unknown	gnl\|CDD\|226870
NZ_CP031065.1\|WP_098364598.1\|5016529_5018341_-\|polysaccharide-biosynthesis-protein	unknown	unknown	gnl\|CDD\|224011
NZ_CP031065.1\|WP_000411274.1\|5031132_5032437_-\|UDP-N-acetylglucosamine-1-carboxyvinyltransferase	unknown	unknown	gnl\|CDD\|236486
NZ_CP031065.1\|WP_149150078.1\|5032476_5033187_-\|hypothetical-protein	unknown	unknown	gnl\|CDD\|370056
NZ_CP031065.1\|WP_149150075.1\|5023124_5023559_-\|3-hydroxyacyl-ACP-dehydratase-FabZ	unknown	unknown	gnl\|CDD\|234568
NZ_CP031065.1\|WP_000057889.1\|5033232_5033469_-\|DUF1146-domain-containing-protein	unknown	unknown	gnl\|CDD\|227173
NZ_CP031065.1\|WP_149150076.1\|5027776_5028619_-\|ATP-binding-cassette-domain-containing-protein	unknown	unknown	gnl\|CDD\|224054
NZ_CP031065.1\|WP_000008901.1\|5025729_5025864_-\|hypothetical-protein	unknown	unknown	unknown
NZ_CP031065.1\|WP_149150073.1\|5021098_5021842_-\|capsular-biosynthesis-protein	unknown	unknown	gnl\|CDD\|226453
NZ_CP031065.1\|WP_149150080.1\|5036692_5038555_-\|NADH-quinone-oxidoreductase-subunit-L	unknown	unknown	gnl\|CDD\|235836
NZ_CP031065.1\|WP_000544012.1\|5025149_5025422_-\|sporulation-transcriptional-regulator-SpoIIID	unknown	unknown	gnl\|CDD\|378813
NZ_CP031065.1\|WP_098364600.1\|5020407_5021109_-\|CpsD/CapB-family-tyrosine-protein-kinase	unknown	unknown	gnl\|CDD\|349772
NZ_CP031065.1\|WP_016123878.1\|5035193_5036696_-\|NADH-quinone-oxidoreductase-subunit-M	unknown	unknown	gnl\|CDD\|235622
NZ_CP031065.1\|WP_149150077.1\|5028800_5029808_-\|ATP-binding-cassette-domain-containing-protein	unknown	unknown	gnl\|CDD\|224054
NZ_CP031065.1\|WP_000457978.1\|5023987_5024989_-\|rod-shape-determining-protein	unknown	unknown	gnl\|CDD\|237563
NZ_CP031065.1\|WP_149150079.1\|5033671_5035192_-\|NADH-quinone-oxidoreductase-subunit-NuoN	unknown	unknown	gnl\|CDD\|235603
NZ_CP031065.1\|WP_000144450.1\|5027072_5027777_-\|ABC-transporter-permease	unknown	unknown	gnl\|CDD\|378925

Protein	Function_ID	Function_description	E-value
NZ_CP031065.1\|WP_000672659.1\|5029906_5030926_-\|stage-II-sporulation-protein-D	gnl\|CDD\|274332	TIGR02870, Stage_II_sporulation_protein_D, stage II sporulation protein D. Stage II sporulation protein D (SpoIID) is a protein of the endospore formation program in a number of lineages in the Firmicutes (low-GC Gram-positive bacteria). It is expressed in the mother cell compartment, under control of Sigma-E. SpoIID, along with SpoIIM and SpoIIP, is one of three major proteins involved in engulfment of the forespore by the mother cell. [Cellular processes, Sporulation and germination].	4.89572e-134
NZ_CP031065.1\|WP_149150074.1\|5022101_5022779_-\|polysaccharide-biosynthesis-tyrosine-autokinase	gnl\|CDD\|349772	cd05387, BY-kinase, bacterial tyrosine-kinase. Bacterial tyrosine (BY)-kinases catalyze the autophosphorylation on a C-terminal tyrosine cluster and also phosphorylate endogenous protein substrates by using ATP as phosphoryl donor. Besides their capacity to function as tyrosine kinase, most of these proteins are also involved in the production and transport of exopolysaccharides. BY-kinases are involved in a number of physiological processes ranging from stress resistance to pathogenicity.	4.56166e-82
NZ_CP031065.1\|WP_098364600.1\|5020407_5021109_-\|CpsD/CapB-family-tyrosine-protein-kinase	gnl\|CDD\|349772	cd05387, BY-kinase, bacterial tyrosine-kinase. Bacterial tyrosine (BY)-kinases catalyze the autophosphorylation on a C-terminal tyrosine cluster and also phosphorylate endogenous protein substrates by using ATP as phosphoryl donor. Besides their capacity to function as tyrosine kinase, most of these proteins are also involved in the production and transport of exopolysaccharides. BY-kinases are involved in a number of physiological processes ranging from stress resistance to pathogenicity.	3.46443e-83
NZ_CP031065.1\|WP_149150072.1\|5019528_5020296_-\|tyrosine-protein-phosphatase	gnl\|CDD\|226870	COG4464, CapC, Capsular polysaccharide biosynthesis protein [Carbohydrate transport and metabolism / Cell envelope biogenesis, outer membrane].	5.1659e-106
NZ_CP031065.1\|WP_098364598.1\|5016529_5018341_-\|polysaccharide-biosynthesis-protein	gnl\|CDD\|224011	COG1086, COG1086, Predicted nucleoside-diphosphate sugar epimerases [Cell envelope biogenesis, outer membrane / Carbohydrate transport and metabolism].	0
NZ_CP031065.1\|WP_000411274.1\|5031132_5032437_-\|UDP-N-acetylglucosamine-1-carboxyvinyltransferase	gnl\|CDD\|236486	PRK09369, PRK09369, UDP-N-acetylglucosamine 1-carboxyvinyltransferase; Validated.	0
NZ_CP031065.1\|WP_149150078.1\|5032476_5033187_-\|hypothetical-protein	gnl\|CDD\|370056	pfam08680, DUF1779, TATA-box binding. TATA-box_bdg is a family of bacterial proteins. YwmB from Bacillus subtilis contains a circularly permuted TATA-box binding protein-like fold. Jian-Xiang Liu, Qi Xie, Jun Lin. Protein Structural Data Mining and Evolutionary Bioinformatic Analysis on Domains of TATA-box Binding Protein-like Fold. Life Science Journal 2014; 11(2): 298-302 (not yet in PubMed 27-02-2014).	5.66666e-34
NZ_CP031065.1\|WP_149150075.1\|5023124_5023559_-\|3-hydroxyacyl-ACP-dehydratase-FabZ	gnl\|CDD\|234568	PRK00006, fabZ, 3-hydroxyacyl-ACP dehydratase FabZ.	5.32075e-95
NZ_CP031065.1\|WP_000057889.1\|5033232_5033469_-\|DUF1146-domain-containing-protein	gnl\|CDD\|227173	COG4836, COG4836, Predicted membrane protein [Function unknown].	1.23409e-23
NZ_CP031065.1\|WP_149150076.1\|5027776_5028619_-\|ATP-binding-cassette-domain-containing-protein	gnl\|CDD\|224054	COG1131, CcmA, ABC-type multidrug transport system, ATPase component [Defense mechanisms].	1.47499e-89
NZ_CP031065.1\|WP_149150073.1\|5021098_5021842_-\|capsular-biosynthesis-protein	gnl\|CDD\|226453	COG3944, COG3944, Capsular polysaccharide biosynthesis protein [Cell envelope biogenesis, outer membrane].	5.27493e-83
NZ_CP031065.1\|WP_149150080.1\|5036692_5038555_-\|NADH-quinone-oxidoreductase-subunit-L	gnl\|CDD\|235836	PRK06590, PRK06590, NADH:ubiquinone oxidoreductase subunit L; Reviewed.	0
NZ_CP031065.1\|WP_000544012.1\|5025149_5025422_-\|sporulation-transcriptional-regulator-SpoIIID	gnl\|CDD\|378813	pfam12116, SpoIIID, Stage III sporulation protein D. This stage III sporulation protein is a small DNA-binding family that is essential for gene expression of the mother-cell compartment during sporulation. The domain is found in bacteria and viruses, and is about 40 amino acids in length. It has a conserved RGG sequence motif.	1.17272e-38
NZ_CP031065.1\|WP_149150130.1\|5018401_5019316_-\|UTP--glucose-1-phosphate-uridylyltransferase-GalU	gnl\|CDD\|224131	COG1210, GalU, UDP-glucose pyrophosphorylase [Cell envelope biogenesis, outer membrane].	0
NZ_CP031065.1\|WP_016123878.1\|5035193_5036696_-\|NADH-quinone-oxidoreductase-subunit-M	gnl\|CDD\|235622	PRK05846, PRK05846, NADH:ubiquinone oxidoreductase subunit M; Reviewed.	6.19585e-178
NZ_CP031065.1\|WP_149150077.1\|5028800_5029808_-\|ATP-binding-cassette-domain-containing-protein	gnl\|CDD\|224054	COG1131, CcmA, ABC-type multidrug transport system, ATPase component [Defense mechanisms].	2.41893e-31
NZ_CP031065.1\|WP_000457978.1\|5023987_5024989_-\|rod-shape-determining-protein	gnl\|CDD\|237563	PRK13928, PRK13928, rod shape-determining protein Mbl; Provisional.	0
NZ_CP031065.1\|WP_149150079.1\|5033671_5035192_-\|NADH-quinone-oxidoreductase-subunit-NuoN	gnl\|CDD\|235603	PRK05777, PRK05777, NADH-quinone oxidoreductase subunit NuoN.	5.15108e-151
NZ_CP031065.1\|WP_000144450.1\|5027072_5027777_-\|ABC-transporter-permease	gnl\|CDD\|378925	pfam12698, ABC2_membrane_3, ABC-2 family transporter protein. This family is related to the ABC-2 membrane transporter family pfam01061.	5.76202e-05

>NZ_CP031065.1|WP_000008901.1|5025729_5025864_-|hypothetical-protein
MSFLFVLSTAIILLFVLFTLYYYIAGKKEVPHHQLLEEEFEREE
>NZ_CP031065.1|WP_000544012.1|5025149_5025422_-|sporulation-transcriptional-regulator-SpoIIID
MHDYIKERTIKIGKYIVETRKTVRVIAKEFGVSKSTVHKDLTERLPEINPELANEVKEILDYHKSIRHLRGGEATKQKYRKEDVEKPVRQ
>NZ_CP031065.1|WP_000457978.1|5023987_5024989_-|rod-shape-determining-protein
MFARDIGIDLGTANVLIHVKGKGIVLNEPSVVAIDRNSGKVLAVGEEARSMVGRTPGNIVAIRPLKDGVIADFEITEAMLKYFINKLDVKSFFSKPRILICCPTNITSVEQKAIREAAERSGGKTVFLEEEPKVAAVGAGMEIFQPSGNMVVDIGGGTTDIAVLSMGDIVTSSSIKMAGDKFDMEILNYIKRKYKLLIGERTSEDIKIKVGTVFPGARSEELEIRGRDMVTGLPRTITVCSEEITEALKENAAVIVQAAKGVLERTPPELSADIIDRGVILTGGGALLHGIDMLLAEELKVPVLIAENPMHCVAVGTGIMLENIDRLPKRALR
>NZ_CP031065.1|WP_149150075.1|5023124_5023559_-|3-hydroxyacyl-ACP-dehydratase-FabZ
MLNIEQIKEIIPHRYPFLLVDKILEVDEGKRAVGIKNVSANEEFFNGHFPDYAVMPGVLIVEALAQVGAVAVLKKEENRGRLAFFAGIDNCRFKKQVRPGDQLRLEVEMTRVRGQIGKGKAIATVDGEVACEAEITFAIGDKKE
>NZ_CP031065.1|WP_149150074.1|5022101_5022779_-|polysaccharide-biosynthesis-tyrosine-autokinase
MFGRKKRKPLRQLITHKEPKSRITEQYRNIRTNIEFTSVDNHVRSIIVTSADPGDGKTTTISNLAVVFGQQGKKVLLIGADLRKPTIQNLFAIHSSNGLTNLLSGQAKLMQCIQKTDIENVYLMAAGPIPPNPAELLGSRAMDETLLEAYNMFDIILIDTPPVLAVTDAQILANKCDGIVLVVRSEKTERDKIIKAKQILDKASGKILGVVLNDKREEKEQYGYY
>NZ_CP031065.1|WP_149150073.1|5021098_5021842_-|capsular-biosynthesis-protein
MEETISLKELFHILKKRLAMILVIAFGAAIVSAIISFFFMTPIYQSSTQILVNQKKQEGAAIQLGEIQSNIQLTNTYKVIIKSPVILEQVNEKLNLNMTAQPLAKKVNVTNEKDSQVISITVEDKDPKVARDIANVTADVFKGEVSKIMNVDNVTVLSKAEVMENQSPIKPAPMLNVAIAFVVGLMASVGLAFLLEYLDNTVKKEEDVENLLGLPVLGIVARMDEETMNVKSHAPSSRKVRGQTIGS
>NZ_CP031065.1|WP_098364600.1|5020407_5021109_-|CpsD/CapB-family-tyrosine-protein-kinase
MALNLFKKKKNHRQRRQLIAHQQPKSPISEQYRNIRTNIEFAAIDTNLHSLMVTSANPSEGKTTTTANMAVVFAQQGKKVLLIDADMRKPAMHQMFQVDNIFGLTNVLTHSERLEKCVQMTSVDNLHFLACGPIPPNPAELLGSKSMKELLGQAYSMYDLVIFDMPPILAVTDAQIMANVCDASILVVRSESTEKETAVKAKGLLESAKGKLLGVVLNDREREQGLYYYYGAN
>NZ_CP031065.1|WP_149150072.1|5019528_5020296_-|tyrosine-protein-phosphatase
MIDLHCHILPGIDDGAQTVTDSLAMAQQAVAEGIHTIVATPHHQNGKYVNERTSIIHHVKQLNDELQQRNIPLTILPGQEVRLYGDLLEDYEAGKIVTLNETNKYIFIEFPSNHVPRYAEQLLYELRVKGMIPIIVHPERNAELIERPDKLYNIVNKGALTQVTAGSLLGKFGKKIKKFSLQLVEYNLTHMVASDAHNITSRGFHLAESYELIEKEFGMNVMSDLKENPYLLISGKAIYKEDPEQIRRKKLFGIF
>NZ_CP031065.1|WP_149150130.1|5018401_5019316_-|UTP--glucose-1-phosphate-uridylyltransferase-GalU
MHIMIYLGVLDLKKVRKAIIPAAGLGTRFLPVTKAMPKEMLPIVDKPTIQYIVEEAIESGIEDIIIVTGKGKRAIEDHFDHSFELEQSLLEKGKHEMLEKVQASSKINIHYIRQKEPKGLGHAVWCARKFIGNEPFAVLLGDDIVQAETPCLRQLMDQYEYTQSSVIGVQTVPENETHRYGIIDPIEQSDRRYQVRQFVEKPAQGTAPSNLAIMGRYVLTPEIFMLLEDQQTGAGGEIQLTDAIQRLNEVQRVFAYDFEGTRYDVGEKFGFIKTTIEMALQNEELNAELMKYMQELVKKEEVHS
>NZ_CP031065.1|WP_098364598.1|5016529_5018341_-|polysaccharide-biosynthesis-protein
MSYRKRLSLLILLDSFIVLTAVYLSYWFIHPNVLSKIPTTVVISSITLLCSHHIFAAIYKLYNKAWEYASIGELKQIFKAVTLSIVVTAIVQQIINHDIYVRILAIAWMLHLLLIGGSRFIWRMFRDTYITKTTDKKRTLIIGAGSAGTMVVRQLQHNKEADLYPIAFVDDDKNKQKLEIYNVPVVGTTNHIQEIVEENDIEHIIIAIPSLNRGQINEIFEKCRKTKAKTQIVPMLEDLLDGKLSVNEFRDVQVEDLLGREPIKLDDAGIGEKITGKTILVTGAGGSIGSEICRQVMKYKPAKIVLLGHGENSIYNIEMEMRVTYKDSVEITTEIADVQDRHKIFEIMKRHQPYIVYHAAAHKHVPLMERNPEEAVKNNIFGTKNVAEAADTFKVNTFVMVSTDKAVNPTNVMGATKRFAEMIVQHMASVSTSTRFVAVRFGNVLGSRGSVIPLFKKQIQKGGPVTVTHPDMIRYFMTIPEASRLVIQAGTLARGGELFVLDMGDPVKIVDLAKNLITLSGFSIEEIGIEFTGLRPGEKMYEELLNEGEIHPEQIFPKIHIGKAVLIDQVILRQFMNDFGEMSKEEIRERLLGIANNKVSIEN
>NZ_CP031065.1|WP_000144450.1|5027072_5027777_-|ABC-transporter-permease
MTFSMRRVSAIFRKEVQDFKTNSQVLLMAFLPIILSFLFSRFGVGKEMLGITTITAFLFVAGFVQSMVIAEEKEKHTLRVLMLSPASSVEVLLGKSLLTSGLTMVICIANLFILDQLNINLPLVGLIFLCGTILFIVLGTMIGLLASSVPQTSIIGMPILMTMYLAVQFEPMVENKVIKTMIGYLPTSHIVKALKSLVGGAGFSSISGHVLNVAVWLIISLVVCLIVYKKKQLD
>NZ_CP031065.1|WP_149150076.1|5027776_5028619_-|ATP-binding-cassette-domain-containing-protein
MTLAIEMKDVMKSFDGKTALRNVNIEVKRGEIFGFLGPSGSGKTTTVKILTSQLLHSIGTVRVLGKDITGPSSIDYKRIGILTDNSGLYERLSIYDNLLLFCDLYDCKKERIDEVLAQVNLLDDKKTPVKKLSKGMKQRVTLARAILHKPDILFLDEPTSALDPVNVQNIHKILKDLNEEGTTIFLTTHNMDEAETLCNRIAFLCGGEIVALDTPENLRLKYAKDHIQVVLKDKQKEVVQKDELGAKRISEWMNKGELLSIHSHEPTLGDIFIEVTGRGL
>NZ_CP031065.1|WP_149150077.1|5028800_5029808_-|ATP-binding-cassette-domain-containing-protein
MALLELKQLGKANQLPEMELKVEKGQCIVLQCNNHTAKVLHRIIIGQEEASTGNVLFEGEAIGKKNYSRIGFCFLKDEAYDRLKVKEYFKFLIGLYESKISIEEVVQSVGLLDKLNVKIEKLSFSEKRRLHIGRVMIHNPDLIILEEPEQNVDIESTVIIRKAIMKMKEQGKAIFITSSFLSDALSLTEDVYILNNDGVKKVEIEQEEVEEVDEEKVVQIIPQMKLERIPAKVNDKIILLDPMEIHFIETQNGVTHIHVREGDFVCTLTLSELEARLTGFGFFRCHRSYLVNLQRVREVITWTRNSFSLILDDERKSSIPLSKGRMDELKGVIGL
>NZ_CP031065.1|WP_000672659.1|5029906_5030926_-|stage-II-sporulation-protein-D
MKFSKPLFITVALLIALVIIVPAALVIPFAKAKVGEETASKTPPAIESIPAPGKVDTAVQVAVYREKQKKVETLPVEEYVTGVVASEMNASFEIEALKAQALAARTFVVQRMLSGGKKNNADVTDTVKDQVYKGKDELKKQWGNNYENNLKKIEEAVSKTAGQVLTYDGKPISASFFSTSNGRTENAADYWGNDYPYLKSVDSPWDQASPKFTSEQTFTVADFQKRLGVKVLADGKVGNIKDLTEGKRVKDVAFQGKTLTGKEVREKLDLRSSDFTWKQQGDKIIVTTKGFGHGVGMSQYGANGMAAEGKKYTDIVAHYYKGVEIKTMNDYEGKLMVKK
>NZ_CP031065.1|WP_000411274.1|5031132_5032437_-|UDP-N-acetylglucosamine-1-carboxyvinyltransferase
MEKIIVRGGKRLNGTVRVEGAKNAVLPIIAAALLASDGKNVLSEVPVLSDVYTINEVLRHLNAEVVFENNQVTIDSSKELNIEAPFEYVRKMRASVQVMGPLLARNGRARIALPGGCAIGSRPIDQHLKGFEAMGAKVQVGNGFVEAYVEGELKGAKIYLDFPSVGATENIMSAATLAKGTTILENAAKEPEIVDLANFLNAMGAKVRGAGTGTIRIEGVDKLYGANHSIIPDRIEAGTFMVAAAITGGDILIENAVPEHLRSITAKMEEMGVKIIEENEGVRVIGPDKLKAVDIKTMPHPGFPTDMQSQMMALLLQADGTSMITETVFENRFMHVEEFRRMNADIKIEGRSVIMNGPNSLQGAEVAATDLRAAAALILAGLVSEGYTRVTELKHLDRGYVNFHKKLAALGATIERVNEKVEEVKEQEVSDLHA
>NZ_CP031065.1|WP_149150078.1|5032476_5033187_-|hypothetical-protein
MKLKLKAILIVALSVVLFLVGYREMKPISDEQKMESMIKALEKNDAKVKKWSWLARETKTISNIHTFQKLLNDVKEKANIQKWEVEQSPDGYKATSYKKLSSYEERVVVTWSKENTKENTFIIFEVSGAKWDPKYVQNMNKIFSEKPIIYTCVQGVLNDKIEGVLQNKTNQVLKDLSARAIEQVEERAFVSVSAYNKKWDDALSTNREKINVQIAIRSTDNKDTIVVGTPIITSEY
>NZ_CP031065.1|WP_000057889.1|5033232_5033469_-|DUF1146-domain-containing-protein
MAQLLGQQALIAIVSHLLFITITWWALQGIHIERLMKSGKVLQTRVLLILITIAIGTSVSNFFLDYLGYSKSLTYLVK
>NZ_CP031065.1|WP_149150079.1|5033671_5035192_-|NADH-quinone-oxidoreductase-subunit-NuoN
MDMNTLLSLSWHLMVPEFIILGAAILLSICDLFFKLNHRYVALGAIAAIVLAIVSLITLYSEPAGDILNGSFVLDGFSKGFKTLLLGASALILCTAMSDDKKNPIEDKGEYYYLFLMALLGAMFMASSVDFITLFVGLELLSLSSYILVGIRKKSRASNEAAMKYVISGGIGTAITLFGMSYLYGITGSTNIVDMQKVITGELASGIQLLLALAFLLLLVGLSFKIATVPFHMWAPDVYEGATTPVTAFLGTISKIAGFLLIIRLFLMIFASVLIQGDMQSLYGHMSIYIAVLASITMIVGNVVALKQYNIKRLFAYSGIAHAGYLLVPLVALSPFTMDSMWFYMLAYMLMNIGAFAIIHGLILQSNEENITIFTGLYKRSPFTAIMMTIFILSLAGIPGTAGFIGKINIFLGALHVEPAHYVLASIMMGTTVVSFVYYFRILQQMFFRTGETEERIRLPLNIKVVMSLCAISIVILGIMPMIGYNFFYEYFPLMKDFFFLGNVVQ
>NZ_CP031065.1|WP_016123878.1|5035193_5036696_-|NADH-quinone-oxidoreductase-subunit-M
MNDLLLTFFIFSPLLGILLLVLTPKKELRTVRALGLFGTALPFGIAIVLACTYASGKNLSLFDEKMKWIKFGDFTAVDKRWFSIYYELGIDGLSLVMMVLTALLATLAAIAAFSIKRNLKAFYMLLLMLEIGMLGVFAAQNLMLFFIFFEITLPPMFLLIGKWGKLSSEKAAYSYLIYNGIGSAILLIVFSILFAKTGTTNIVELKEILRSVNAEGALVIPSSLQLSVFIAIMIAFAIKLPVFPLHRWMVNVHIEAHPAVVMLHAGVLLKIGAYGIIRFGKGLFPEYFHDVATLIAILGVINLLYGAFLALIQTDFRKVLAYSSISHMGIVLMGLAALNAPGTQGALFQVVSHGLIAALLFFLLGVIEQRFGTSDITALGGLAKSVPVLSGFFLAGGMASLGLPGMSGFVSEFLAFLGLFQGEPVIAAVGVIGIILTAVYVLRATLQVTFGKKEWEAKSDIHGWEYVPVLLLIFCIIAIGVMPEILGDPLQNTLKTLGVK
>NZ_CP031065.1|WP_149150080.1|5036692_5038555_-|NADH-quinone-oxidoreductase-subunit-L
MIDYAWLIPLFPLVSFLLLIIFGKKIREGSSVLSIFFVFLSFILAVLVLIERFSEASVKHKWVWLRAGDVDISFGFEVTALGALMLFIVTLVSLLVHVYSKGYMKGDERLPTFYAYLGLFTFAMLGLVISTNLLQLYIFWELVGLGSFLLIGFYFFKEEAKAAAKKAFIMTRIGDVGLFIGMILIFWYAGSFEYDAIFRAIYTGDLPPYMITITAILIFIGAMGKSGQFPLHTWLPDAMEGPTPVSALIHAATMVAAGVYLVATMFPLFSASTVAMQTVAIVGAFTAIFAASIGLVQTDIKRVLAYSTVSQLGYMMLALGSAGYVAGIFHLTTHAFFKALLFLAAGSVIHAVHTQNINKMGGLQKKMKVTGTLFLIGTLAISGVPLLSGFFSKDEILAATWMNGNYVLFTLAVIAAFLTAFYMFRLYFLVFTGETKTKEEVHESPRVMTYPMIVLGVLAVVAGYINTPWFGTFLGDWLTKDVGFKVEETHGPVWIMIVATLVSFAGIALAYFIYRKKSISGNWAGGTETSLYNLLKEKYYVDELYNMTVIPITKGIAHVLRLFEVYVVEGIAVLIASVVKGTSNLGSKLQNGNVQVYGTVTAVSLAVLVIILLYTGGDLR

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Click the colored protein region to show detailed information

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity
NZ_CP031065_1	1.6\|3143256\|25\|NZ_CP031065\|CRT	3143256-3143280	25	MT774376	CrAssphage cr55_1, complete genome	95986-96010	4	0.84
NZ_CP031065_3	3.1\|5026075\|31\|NZ_CP031065\|CRISPRCasFinder	5026075-5026105	31	JX238501	Bacillus phage phiAGATE, complete genome	124559-124589	8	0.742
NZ_CP031065_1	1.8\|3143364\|34\|NZ_CP031065\|CRT	3143364-3143397	34	MT820023	Cryophage ML09, complete genome	23671-23704	12	0.647

1. spacer 1.6|3143256|25|NZ_CP031065|CRT matches to MT774376 (CrAssphage cr55_1, complete genome) position: , mismatch: 4, identity: 0.84

cattactccttgtggtccttgaatt	CRISPR spacer
aggttctccttgtggtccttgaatt	Protospacer
 . * ********************

2. spacer 3.1|5026075|31|NZ_CP031065|CRISPRCasFinder matches to JX238501 (Bacillus phage phiAGATE, complete genome) position: , mismatch: 8, identity: 0.742

ttcttcttttttgtcactagttgaaccgctt	CRISPR spacer
ctaaccttttttgccactagttaaaccgccc	Protospacer
.*  .********.********.******..

3. spacer 1.8|3143364|34|NZ_CP031065|CRT matches to MT820023 (Cryophage ML09, complete genome) position: , mismatch: 12, identity: 0.647

ggttgggcctatacttccttgtggtccctgaata	CRISPR spacer
ttgtgcgcctgtacttccttgtggtcctacgggg	Protospacer
   ** ****.****************.  .. .

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

256670 : 264619

uncultured_virus(33.33%)

The bacterium proteins that are colored denote the protein is present at specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin' and 'tRNA')

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_000917311.1\|256670_256955_+	co-chaperone GroES	A0A221S3C8	uncultured_virus	1.2e-20	54.8
WP_001029993.1\|256993_258628_+	chaperonin GroEL	A0A219YK78	uncultured_virus	1.5e-157	58.0
WP_000743905.1\|259035_260574_+	glutamine-hydrolyzing GMP synthase	A0A1V0SH76	Hokovirus	8.8e-22	31.7
WP_000833096.1\|260958_262284_+	NCS2 family permease	A0A0R6PHV4	Moraxella_phage	4.1e-44	29.4
WP_000929885.1\|262428_263130_+	response regulator transcription factor	W8CYM9	Bacillus_phage	7.3e-40	36.9
WP_000719205.1\|263113_264619_+	aminopeptidase AmpS	W8CYF6	Bacillus_phage	5.4e-32	30.3

DBSCAN-SWA_2

310195 : 318571

Synechococcus_phage(50.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_000625682.1\|310195_311503_+	adenylosuccinate lyase	A0A2H4UUU6	Bodo_saltans_virus	4.9e-21	25.3
WP_001170544.1\|311591_312311_+	phosphoribosylaminoimidazolesuccinocarboxamide synthase	G8EYA2	Synechococcus_phage	4.0e-49	44.3
WP_000278823.1\|312303_312558_+	phosphoribosylformylglycinamidine synthase subunit PurS	NA	NA	NA	NA
WP_000666786.1\|312554_313238_+	phosphoribosylformylglycinamidine synthase subunit PurQ	NA	NA	NA	NA
WP_000055564.1\|313221_315441_+	phosphoribosylformylglycinamidine synthase II	A6N228	Microbacterium_phage	2.0e-163	41.2
WP_000879025.1\|315425_316841_+	amidophosphoribosyltransferase	A0A0M3SGR2	Mollivirus	3.3e-55	35.4
WP_001262439.1\|316946_317987_+	phosphoribosylformylglycinamidine cyclo-ligase	A0A0E3F760	Synechococcus_phage	1.2e-67	45.9
WP_000088589.1\|317983_318571_+	phosphoribosylglycinamide formyltransferase	M4QRX9	Synechococcus_phage	9.1e-28	37.4

DBSCAN-SWA_3

649212 : 657209

Bacillus_phage(66.67%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_116269190.1\|649212_650181_-	YafY family transcriptional regulator	A0A1B0RXM1	Streptococcus_phage	1.4e-17	29.9
WP_000085783.1\|650260_650782_+	dihydrofolate reductase	NA	NA	NA	NA
WP_006918010.1\|650795_652187_-	HAMP domain-containing histidine kinase	W8CYF6	Bacillus_phage	1.1e-31	29.2
WP_006918011.1\|652198_652876_-	response regulator transcription factor	W8CYM9	Bacillus_phage	1.1e-32	35.6
WP_149149323.1\|653051_654299_+	cell wall-binding repeat-containing protein	NA	NA	NA	NA
WP_000277058.1\|654432_654963_+	DUF4352 domain-containing protein	A0A0S2MVG4	Bacillus_phage	3.6e-15	33.7
WP_000831286.1\|654975_655320_+	DUF4064 domain-containing protein	A0A0S2MVJ2	Bacillus_phage	8.5e-42	78.1
WP_000487920.1\|655757_657209_+	SulP family inorganic anion transporter	A0A2H4J153	uncultured_Caudovirales_phage	4.4e-140	54.2

DBSCAN-SWA_4

1798969 : 1807075

Bacillus_phage(66.67%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_000755496.1\|1798969_1800268_+	C40 family peptidase	A0A1V0DZX6	Clostridioides_phage	3.5e-11	31.5
WP_001194309.1\|1800367_1801132_-	alpha/beta hydrolase	NA	NA	NA	NA
WP_049107614.1\|1801372_1803133_+	ABC transporter ATP-binding protein	W8CYL7	Bacillus_phage	3.1e-273	98.2
WP_000612414.1\|1803218_1803896_+	response regulator transcription factor	W8CYM9	Bacillus_phage	1.1e-122	99.1
WP_049107615.1\|1803892_1804966_+	HAMP domain-containing sensor histidine kinase	W8CYF6	Bacillus_phage	1.6e-187	98.3
WP_000818985.1\|1805193_1805913_+	3-ketoacyl-ACP reductase	W8CYX9	Bacillus_phage	5.9e-61	100.0
WP_001258539.1\|1806202_1807075_+	aminoglycoside 6-adenylyltransferase	E4ZFP8	Streptococcus_phage	2.8e-65	44.4

DBSCAN-SWA_5

2429501 : 2472074

Bacillus_phage(60.53%)

portal,integrase,head,capsid,terminase,tail,bacteriocin,holin,protease

attL 2424790:2424805|attR 2458995:2459010

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
2424790:2424805	attL	NA	NA	NA	NA
WP_001071364.1\|2429501_2429822_+\|bacteriocin	heterocycloanthracin/sonorensin family bacteriocin	NA	NA	NA	NA
WP_002024190.1\|2430311_2430797_+	hypothetical protein	NA	NA	NA	NA
WP_000736205.1\|2431109_2431811_+	DUF3962 domain-containing protein	NA	NA	NA	NA
WP_000675852.1\|2431849_2432959_-\|integrase	site-specific integrase	A0A0U3U8Y8	Bacillus_phage	5.7e-148	79.7
WP_149149632.1\|2433622_2434831_+	hypothetical protein	D2XQ10	Bacillus_virus	4.7e-79	44.7
WP_102956497.1\|2435273_2435624_-	helix-turn-helix transcriptional regulator	A0A288WFW4	Bacillus_phage	3.3e-17	39.7
WP_149149633.1\|2435806_2436103_+	helix-turn-helix domain-containing protein	A0A288WG89	Bacillus_phage	1.8e-08	46.5
WP_102956499.1\|2436065_2436278_+	hypothetical protein	A0A288WFT2	Bacillus_phage	5.4e-07	50.0
WP_098520263.1\|2436318_2436585_+	helix-turn-helix domain-containing protein	A0A0U3ULL9	Bacillus_phage	1.2e-35	90.6
WP_149149634.1\|2436959_2437676_+	DnaD domain protein	A0A1B0T6C0	Bacillus_phage	2.1e-79	80.7
WP_149149635.1\|2437614_2438490_+	ATP-binding protein	Q2I8C8	Bacillus_phage	4.2e-61	44.5
WP_016124626.1\|2438505_2438700_+	hypothetical protein	A0A1B1P7U7	Bacillus_phage	4.3e-27	89.1
WP_000805174.1\|2438725_2438899_+	DUF3954 domain-containing protein	A0A1B1P7U5	Bacillus_phage	1.2e-25	100.0
WP_149149636.1\|2438913_2439168_+	hypothetical protein	A0A0U3SU43	Bacillus_phage	1.7e-34	82.1
WP_149149637.1\|2439176_2439641_+	nucleoside permease	A0A2H4J8I5	uncultured_Caudovirales_phage	4.4e-17	35.4
WP_149150116.1\|2439836_2440121_+	hypothetical protein	NA	NA	NA	NA
WP_098398431.1\|2440011_2440677_-	hypothetical protein	NA	NA	NA	NA
WP_149149638.1\|2440805_2441336_-	GNAT family N-acetyltransferase	NA	NA	NA	NA
WP_149149639.1\|2441579_2442206_-	exosporium leader peptide-containing protein	NA	NA	NA	NA
WP_086385656.1\|2442326_2442599_-	hypothetical protein	NA	NA	NA	NA
WP_149149640.1\|2442745_2443198_-	epimerase	NA	NA	NA	NA
WP_149150117.1\|2443363_2444068_+	site-specific DNA-methyltransferase	M4M8X6	Vibrio_phage	3.1e-75	61.3
WP_149149641.1\|2444584_2444881_-	hypothetical protein	NA	NA	NA	NA
WP_149149642.1\|2445309_2445792_+	ArpU family transcriptional regulator	H0USV3	Bacillus_phage	4.6e-70	81.9
WP_149149643.1\|2445791_2446334_+\|integrase	tyrosine-type recombinase/integrase	W8CYP1	Bacillus_phage	1.0e-86	88.9
WP_149149644.1\|2446539_2447379_+	DUF2935 domain-containing protein	A0A167RKB4	Powai_lake_megavirus	1.5e-07	25.7
WP_149149645.1\|2447749_2448172_+	hypothetical protein	NA	NA	NA	NA
WP_065845245.1\|2448625_2449177_+	hypothetical protein	A0A288WFY4	Bacillus_phage	2.0e-32	41.5
WP_080478490.1\|2449257_2449563_+	hypothetical protein	NA	NA	NA	NA
WP_065845247.1\|2449834_2450314_+	hypothetical protein	NA	NA	NA	NA
WP_149248986.1\|2450361_2450562_+	hypothetical protein	NA	NA	NA	NA
WP_065845249.1\|2450562_2450820_+	hydrolase	NA	NA	NA	NA
WP_065845250.1\|2450812_2451148_+	HNH endonuclease	D2XR61	Bacillus_phage	3.4e-51	88.3
WP_000763336.1\|2451302_2451659_+	hypothetical protein	A0A2H4JBV9	uncultured_Caudovirales_phage	3.6e-59	100.0
WP_149149646.1\|2451655_2453311_+\|terminase	terminase large subunit	A0A2H4JI41	uncultured_Caudovirales_phage	8.7e-310	95.1
WP_149150118.1\|2453337_2454483_+\|portal	phage portal protein	A0A2H4JAJ1	uncultured_Caudovirales_phage	7.9e-185	85.2
WP_149149647.1\|2454466_2455243_+\|protease	Clp protease ClpP	A0A0A7RWX3	Clostridium_phage	1.1e-55	55.8
WP_149149648.1\|2455263_2456418_+\|capsid	phage major capsid protein	A0A2H4JH29	uncultured_Caudovirales_phage	1.6e-185	85.1
WP_149149649.1\|2456423_2456717_+	hypothetical protein	D2XR19	Bacillus_phage	5.9e-44	91.8
WP_149149650.1\|2456718_2457072_+\|head	phage head closure protein	A0A2H4JGG7	uncultured_Caudovirales_phage	5.1e-58	95.7
WP_149149651.1\|2457073_2457418_+	HK97 gp10 family phage protein	D2XR21	Bacillus_phage	3.4e-51	92.0
WP_149149652.1\|2457414_2457744_+	hypothetical protein	D2XR22	Bacillus_phage	1.3e-52	94.5
WP_001004911.1\|2457744_2458332_+\|tail	phage tail protein	A0A2H4JDV4	uncultured_Caudovirales_phage	3.0e-87	82.6
WP_149149653.1\|2458338_2458701_+	hypothetical protein	A0A2H4JAG8	uncultured_Caudovirales_phage	4.7e-51	80.8
WP_140335780.1\|2458697_2458916_+	hypothetical protein	A0A2H4JHE4	uncultured_Caudovirales_phage	2.8e-22	92.5
WP_149149654.1\|2458931_2460143_+	hypothetical protein	A0A2H4JAI2	uncultured_Caudovirales_phage	7.3e-189	86.6
2458995:2459010	attR	NA	NA	NA	NA
WP_149149655.1\|2460396_2460657_+	hypothetical protein	A0A1B1P763	Bacillus_phage	1.3e-34	85.9
WP_149149656.1\|2460871_2463040_+\|tail	phage tail tape measure protein	A0A2H4JC82	uncultured_Caudovirales_phage	2.3e-84	83.5
WP_149149657.1\|2463081_2464539_+\|tail	phage tail family protein	A0A0A7AQV1	Bacillus_phage	1.3e-171	59.5
WP_149149658.1\|2464535_2468855_+	hypothetical protein	A0A0S2MVB4	Bacillus_phage	0.0e+00	59.1
WP_149149659.1\|2468866_2469247_+	hypothetical protein	A0A0S2MVE0	Bacillus_phage	5.3e-45	74.2
WP_149149660.1\|2469365_2470751_+	PQQ-binding-like beta-propeller repeat protein	NA	NA	NA	NA
WP_065703361.1\|2470839_2471265_+\|holin	phage holin family protein	A0A1B1P787	Bacillus_phage	1.4e-70	96.5
WP_149149661.1\|2471264_2472074_+	N-acetylmuramoyl-L-alanine amidase	D2XR33	Bacillus_phage	4.8e-152	92.2

DBSCAN-SWA_6

3514195 : 3524368

Bacillus_phage(54.55%)

bacteriocin

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_000413738.1\|3514195_3514816_+	transcriptional repressor LexA	A0A1B2APZ1	Phage_Wrath	8.2e-19	62.2
WP_048536031.1\|3514906_3515710_-	DUF4097 family beta strand repeat protein	NA	NA	NA	NA
WP_000031383.1\|3515710_3516253_-	DUF1700 domain-containing protein	NA	NA	NA	NA
WP_001102628.1\|3516245_3516569_-	PadR family transcriptional regulator	NA	NA	NA	NA
WP_000392444.1\|3516940_3517171_-\|bacteriocin	bacteriocin biosynthesis protein	A0A1B1P7Q9	Bacillus_phage	1.5e-23	73.7
WP_016123244.1\|3517232_3518129_-	hypothetical protein	A0A0S2MVR5	Bacillus_phage	4.6e-79	52.1
WP_006920050.1\|3518401_3519244_-	M23 family metallopeptidase	A0A218KCJ1	Bacillus_phage	6.1e-33	47.7
WP_033692154.1\|3519362_3520349_-	lysozyme family protein	A0A223LKM8	Bacillus_phage	1.7e-34	43.9
WP_000464425.1\|3520587_3520974_-	DUF1492 domain-containing protein	A0A288WG73	Bacillus_phage	2.6e-47	71.4
WP_149149878.1\|3521092_3521974_-	DnaD domain protein	A0A2H4J394	uncultured_Caudovirales_phage	5.3e-96	65.9
WP_001189065.1\|3522126_3522321_-	hypothetical protein	A0A1C8E9A1	Bacillus_phage	1.7e-15	71.0
WP_086412708.1\|3522332_3523091_-	phage regulatory protein	A0A2H4JBC7	uncultured_Caudovirales_phage	2.0e-96	69.0
WP_000283431.1\|3523293_3523506_-	hypothetical protein	A6M975	Geobacillus_virus	1.6e-11	63.0
WP_006920059.1\|3523696_3524368_+	helix-turn-helix domain-containing protein	A0A2H4J441	uncultured_Caudovirales_phage	6.8e-35	41.0

DBSCAN-SWA_7

4311663 : 4319350

Staphylococcus_phage(16.67%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_017674010.1\|4311663_4312587_-	ABC transporter ATP-binding protein	A0A2H4PQG7	Staphylococcus_phage	4.0e-46	44.0
WP_016125943.1\|4312713_4313649_-	HAMP domain-containing histidine kinase	Q8QNA2	Ectocarpus_siliculosus_virus	4.3e-11	25.9
WP_149149936.1\|4313650_4314343_-	response regulator	B5LWA6	Feldmannia_species_virus	1.4e-06	29.8
WP_149149937.1\|4314685_4314880_+	YwbE family protein	NA	NA	NA	NA
WP_006916399.1\|4314918_4316118_-	class I SAM-dependent rRNA methyltransferase	W6LLI2	Streptococcus_phage	4.7e-71	41.2
WP_000587818.1\|4316413_4316737_+	heme oxygenase	NA	NA	NA	NA
WP_001086120.1\|4316809_4317574_-	class B sortase	NA	NA	NA	NA
WP_000403758.1\|4317606_4318377_-	ABC transporter ATP-binding protein	W6JKT0	Anomala_cuprea_entomopoxvirus	3.7e-13	27.1
WP_001036848.1\|4318366_4319350_-	iron ABC transporter permease	A0A2H4IY97	uncultured_Caudovirales_phage	1.1e-17	27.2

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

Overview of predicted results

Overview of the results

Cas Category Instructions

Results visualization

1. NZ_CP031065

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Click the colored protein region to show detailed information

Click the colored protein region to show detailed information

Click the colored protein region to show detailed information

Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection